PMC:1208873 JSON TXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id	Subject	Object	Predicate	Lexical cue
T204	0-12	NN	denotes	Inactivation
T206	0-126	sentence	denotes	Inactivation of the Huntington's disease gene (Hdh) impairs anterior streak formation and early patterning of the mouse embryo
T207	13-15	IN	denotes	of
T208	16-19	DT	denotes	the
T210	20-30	NNP	denotes	Huntington
T212	30-32	POS	denotes	's
T211	33-40	NN	denotes	disease
T209	41-45	NN	denotes	gene
T213	46-47	-LRB-	denotes	(
T214	47-50	NN	denotes	Hdh
T215	50-51	-RRB-	denotes	)
T205	52-59	VBZ	denotes	impairs
T216	60-68	JJ	denotes	anterior
T218	69-75	NN	denotes	streak
T217	76-85	NN	denotes	formation
T219	86-89	CC	denotes	and
T220	90-95	JJ	denotes	early
T221	96-106	NN	denotes	patterning
T222	107-109	IN	denotes	of
T223	110-113	DT	denotes	the
T225	114-119	NN	denotes	mouse
T224	120-126	NN	denotes	embryo
T226	126-127	sentence	denotes
T227	137-147	sentence	denotes	Background
T228	137-147	NN	denotes	Background
T229	147-390	sentence	denotes	Huntingtin, the HD gene encoded protein mutated by polyglutamine expansion in Huntington's disease, is required in extraembryonic tissues for proper gastrulation, implicating its activities in nutrition or patterning of the developing embryo.
T230	148-158	NN	denotes	Huntingtin
T232	158-160	,	denotes	,
T233	160-163	DT	denotes	the
T235	164-166	NN	denotes	HD
T236	167-171	NN	denotes	gene
T237	172-179	VBN	denotes	encoded
T234	180-187	NN	denotes	protein
T238	188-195	VBN	denotes	mutated
T239	196-198	IN	denotes	by
T240	199-212	NN	denotes	polyglutamine
T241	213-222	NN	denotes	expansion
T242	223-225	IN	denotes	in
T243	226-236	NNP	denotes	Huntington
T245	236-238	POS	denotes	's
T244	239-246	NN	denotes	disease
T246	246-248	,	denotes	,
T247	248-250	VBZ	denotes	is
T231	251-259	VBN	denotes	required
T248	260-262	IN	denotes	in
T249	263-277	JJ	denotes	extraembryonic
T250	278-285	NNS	denotes	tissues
T251	286-289	IN	denotes	for
T252	290-296	JJ	denotes	proper
T253	297-309	NN	denotes	gastrulation
T254	309-311	,	denotes	,
T255	311-322	VBG	denotes	implicating
T256	323-326	PRP$	denotes	its
T257	327-337	NNS	denotes	activities
T258	338-340	IN	denotes	in
T259	341-350	NN	denotes	nutrition
T260	351-353	CC	denotes	or
T261	354-364	NN	denotes	patterning
T262	365-367	IN	denotes	of
T263	368-371	DT	denotes	the
T265	372-382	VBG	denotes	developing
T264	383-389	NN	denotes	embryo
T266	389-390	.	denotes	.
T267	390-569	sentence	denotes	To test these possibilities, we have used whole mount in situ hybridization to examine embryonic patterning and morphogenesis in homozygous Hdhex4/5 huntingtin deficient embryos.
T268	391-393	TO	denotes	To
T269	394-398	VB	denotes	test
T271	399-404	DT	denotes	these
T272	405-418	NNS	denotes	possibilities
T273	418-420	,	denotes	,
T274	420-422	PRP	denotes	we
T275	423-427	VBP	denotes	have
T270	428-432	VBN	denotes	used
T276	433-438	JJ	denotes	whole
T277	439-444	NN	denotes	mount
T279	445-447	FW	denotes	in
T280	448-452	FW	denotes	situ
T278	453-466	NN	denotes	hybridization
T281	467-469	TO	denotes	to
T282	470-477	VB	denotes	examine
T283	478-487	JJ	denotes	embryonic
T284	488-498	NN	denotes	patterning
T285	499-502	CC	denotes	and
T286	503-516	NN	denotes	morphogenesis
T287	517-519	IN	denotes	in
T288	520-530	JJ	denotes	homozygous
T290	531-537	NN	denotes	Hdhex4
T291	537-538	HYPH	denotes	/
T292	538-539	CD	denotes	5
T293	540-550	NN	denotes	huntingtin
T294	551-560	JJ	denotes	deficient
T289	561-568	NNS	denotes	embryos
T295	568-569	.	denotes	.
T296	569-578	sentence	denotes	Results
T297	571-578	NNS	denotes	Results
T298	578-726	sentence	denotes	In the absence of huntingtin, expression of nutritive genes appears normal but E7.0–7.5 embryos exhibit a unique combination of patterning defects.
T299	579-581	IN	denotes	In
T301	582-585	DT	denotes	the
T302	586-593	NN	denotes	absence
T303	594-596	IN	denotes	of
T304	597-607	NN	denotes	huntingtin
T305	607-609	,	denotes	,
T306	609-619	NN	denotes	expression
T307	620-622	IN	denotes	of
T308	623-632	JJ	denotes	nutritive
T309	633-638	NNS	denotes	genes
T300	639-646	VBZ	denotes	appears
T310	647-653	JJ	denotes	normal
T311	654-657	CC	denotes	but
T312	658-662	NN	denotes	E7.0
T314	662-663	SYM	denotes	–
T315	663-666	CD	denotes	7.5
T313	667-674	NNS	denotes	embryos
T316	675-682	VBP	denotes	exhibit
T317	683-684	DT	denotes	a
T319	685-691	JJ	denotes	unique
T318	692-703	NN	denotes	combination
T320	704-706	IN	denotes	of
T321	707-717	NN	denotes	patterning
T322	718-725	NNS	denotes	defects
T323	725-726	.	denotes	.
T324	726-851	sentence	denotes	Notable are a shortened primitive streak, absence of a proper node and diminished production of anterior streak derivatives.
T325	727-734	JJ	denotes	Notable
T326	735-738	VBP	denotes	are
T327	739-740	DT	denotes	a
T329	741-750	VBN	denotes	shortened
T330	751-760	JJ	denotes	primitive
T328	761-767	NN	denotes	streak
T331	767-769	,	denotes	,
T332	769-776	NN	denotes	absence
T333	777-779	IN	denotes	of
T334	780-781	DT	denotes	a
T336	782-788	JJ	denotes	proper
T335	789-793	NN	denotes	node
T337	794-797	CC	denotes	and
T338	798-808	VBN	denotes	diminished
T339	809-819	NN	denotes	production
T340	820-822	IN	denotes	of
T341	823-831	JJ	denotes	anterior
T342	832-838	NN	denotes	streak
T343	839-850	NNS	denotes	derivatives
T344	850-851	.	denotes	.
T345	851-1015	sentence	denotes	Reduced Wnt3a, Tbx6 and Dll1 expression signify decreased paraxial mesoderm and reduced Otx2 expression and lack of headfolds denote a failure of head development.
T346	852-859	VBN	denotes	Reduced
T348	860-865	NN	denotes	Wnt3a
T349	865-867	,	denotes	,
T350	867-871	NN	denotes	Tbx6
T351	872-875	CC	denotes	and
T352	876-880	NN	denotes	Dll1
T347	881-891	NN	denotes	expression
T353	892-899	VBP	denotes	signify
T354	900-909	VBN	denotes	decreased
T356	910-918	JJ	denotes	paraxial
T355	919-927	NN	denotes	mesoderm
T357	928-931	CC	denotes	and
T358	932-939	VBN	denotes	reduced
T360	940-944	NN	denotes	Otx2
T359	945-955	NN	denotes	expression
T361	956-959	CC	denotes	and
T362	960-964	NN	denotes	lack
T364	965-967	IN	denotes	of
T365	968-977	NNS	denotes	headfolds
T363	978-984	VBP	denotes	denote
T366	985-986	DT	denotes	a
T367	987-994	NN	denotes	failure
T368	995-997	IN	denotes	of
T369	998-1002	NN	denotes	head
T370	1003-1014	NN	denotes	development
T371	1014-1015	.	denotes	.
T372	1015-1247	sentence	denotes	In addition, genes initially broadly expressed are not properly restricted to the posterior, as evidenced by the ectopic expression of Nodal, Fgf8 and Gsc in the epiblast and T (Brachyury) and Evx1 in proximal mesoderm derivatives.
T373	1016-1018	IN	denotes	In
T375	1019-1027	NN	denotes	addition
T376	1027-1029	,	denotes	,
T377	1029-1034	NNS	denotes	genes
T378	1035-1044	RB	denotes	initially
T380	1045-1052	RB	denotes	broadly
T379	1053-1062	VBN	denotes	expressed
T381	1063-1066	VBP	denotes	are
T382	1067-1070	RB	denotes	not
T383	1071-1079	RB	denotes	properly
T374	1080-1090	VBN	denotes	restricted
T384	1091-1093	IN	denotes	to
T385	1094-1097	DT	denotes	the
T386	1098-1107	JJ	denotes	posterior
T387	1107-1109	,	denotes	,
T388	1109-1111	IN	denotes	as
T389	1112-1121	VBN	denotes	evidenced
T390	1122-1124	IN	denotes	by
T391	1125-1128	DT	denotes	the
T393	1129-1136	JJ	denotes	ectopic
T392	1137-1147	NN	denotes	expression
T394	1148-1150	IN	denotes	of
T395	1151-1156	NN	denotes	Nodal
T396	1156-1158	,	denotes	,
T397	1158-1162	NN	denotes	Fgf8
T398	1163-1166	CC	denotes	and
T399	1167-1170	NN	denotes	Gsc
T400	1171-1173	IN	denotes	in
T401	1174-1177	DT	denotes	the
T402	1178-1186	NN	denotes	epiblast
T403	1187-1190	CC	denotes	and
T404	1191-1192	NN	denotes	T
T406	1193-1194	-LRB-	denotes	(
T405	1194-1203	NNP	denotes	Brachyury
T407	1203-1204	-RRB-	denotes	)
T408	1205-1208	CC	denotes	and
T409	1209-1213	NN	denotes	Evx1
T410	1214-1216	IN	denotes	in
T411	1217-1225	JJ	denotes	proximal
T413	1226-1234	NN	denotes	mesoderm
T412	1235-1246	NNS	denotes	derivatives
T414	1246-1247	.	denotes	.
T415	1247-1368	sentence	denotes	Despite impaired posterior restriction and anterior streak deficits, overall anterior/posterior polarity is established.
T416	1248-1255	IN	denotes	Despite
T418	1256-1264	VBN	denotes	impaired
T420	1265-1274	JJ	denotes	posterior
T419	1275-1286	NN	denotes	restriction
T421	1287-1290	CC	denotes	and
T422	1291-1299	JJ	denotes	anterior
T423	1300-1306	NN	denotes	streak
T424	1307-1315	NNS	denotes	deficits
T425	1315-1317	,	denotes	,
T426	1317-1324	JJ	denotes	overall
T428	1325-1333	JJ	denotes	anterior
T430	1333-1334	HYPH	denotes	/
T429	1334-1343	JJ	denotes	posterior
T427	1344-1352	NN	denotes	polarity
T431	1353-1355	VBZ	denotes	is
T417	1356-1367	VBN	denotes	established
T432	1367-1368	.	denotes	.
T433	1368-1507	sentence	denotes	A single primitive streak forms and marker expression shows that the anterior epiblast and anterior visceral endoderm (AVE) are specified.
T434	1369-1370	DT	denotes	A
T436	1371-1377	JJ	denotes	single
T437	1378-1387	JJ	denotes	primitive
T435	1388-1394	NN	denotes	streak
T438	1395-1400	VBZ	denotes	forms
T439	1401-1404	CC	denotes	and
T440	1405-1411	NN	denotes	marker
T441	1412-1422	NN	denotes	expression
T442	1423-1428	VBZ	denotes	shows
T443	1429-1433	IN	denotes	that
T445	1434-1437	DT	denotes	the
T447	1438-1446	JJ	denotes	anterior
T446	1447-1455	NN	denotes	epiblast
T448	1456-1459	CC	denotes	and
T449	1460-1468	JJ	denotes	anterior
T451	1469-1477	JJ	denotes	visceral
T450	1478-1486	NN	denotes	endoderm
T452	1487-1488	-LRB-	denotes	(
T453	1488-1491	NN	denotes	AVE
T454	1491-1492	-RRB-	denotes	)
T455	1493-1496	VBP	denotes	are
T444	1497-1506	VBN	denotes	specified
T456	1506-1507	.	denotes	.
T457	1507-1519	sentence	denotes	Conclusion
T458	1509-1519	NN	denotes	Conclusion
T459	1519-1729	sentence	denotes	Huntingtin is essential in the early patterning of the embryo for formation of the anterior region of the primitive streak, and for down-regulation of a subset of dynamic growth and transcription factor genes.
T460	1520-1530	NN	denotes	Huntingtin
T461	1531-1533	VBZ	denotes	is
T462	1534-1543	JJ	denotes	essential
T463	1544-1546	IN	denotes	in
T464	1547-1550	DT	denotes	the
T466	1551-1556	JJ	denotes	early
T465	1557-1567	NN	denotes	patterning
T467	1568-1570	IN	denotes	of
T468	1571-1574	DT	denotes	the
T469	1575-1581	NN	denotes	embryo
T470	1582-1585	IN	denotes	for
T471	1586-1595	NN	denotes	formation
T472	1596-1598	IN	denotes	of
T473	1599-1602	DT	denotes	the
T475	1603-1611	JJ	denotes	anterior
T474	1612-1618	NN	denotes	region
T476	1619-1621	IN	denotes	of
T477	1622-1625	DT	denotes	the
T479	1626-1635	JJ	denotes	primitive
T478	1636-1642	NN	denotes	streak
T480	1642-1644	,	denotes	,
T481	1644-1647	CC	denotes	and
T482	1648-1651	IN	denotes	for
T483	1652-1656	RP	denotes	down
T485	1656-1657	HYPH	denotes	-
T484	1657-1667	NN	denotes	regulation
T486	1668-1670	IN	denotes	of
T487	1671-1672	DT	denotes	a
T488	1673-1679	NN	denotes	subset
T489	1680-1682	IN	denotes	of
T490	1683-1690	JJ	denotes	dynamic
T492	1691-1697	NN	denotes	growth
T494	1698-1701	CC	denotes	and
T495	1702-1715	NN	denotes	transcription
T493	1716-1722	NN	denotes	factor
T491	1723-1728	NNS	denotes	genes
T496	1728-1729	.	denotes	.
T497	1729-1932	sentence	denotes	These findings provide fundamental starting points for identifying the novel cellular and molecular activities of huntingtin in the extraembryonic tissues that govern normal anterior streak development.
T498	1730-1735	DT	denotes	These
T499	1736-1744	NNS	denotes	findings
T500	1745-1752	VBP	denotes	provide
T501	1753-1764	JJ	denotes	fundamental
T503	1765-1773	VBG	denotes	starting
T502	1774-1780	NNS	denotes	points
T504	1781-1784	IN	denotes	for
T505	1785-1796	VBG	denotes	identifying
T506	1797-1800	DT	denotes	the
T508	1801-1806	JJ	denotes	novel
T509	1807-1815	JJ	denotes	cellular
T510	1816-1819	CC	denotes	and
T511	1820-1829	JJ	denotes	molecular
T507	1830-1840	NNS	denotes	activities
T512	1841-1843	IN	denotes	of
T513	1844-1854	NN	denotes	huntingtin
T514	1855-1857	IN	denotes	in
T515	1858-1861	DT	denotes	the
T517	1862-1876	JJ	denotes	extraembryonic
T516	1877-1884	NNS	denotes	tissues
T518	1885-1889	WDT	denotes	that
T519	1890-1896	VBP	denotes	govern
T520	1897-1903	JJ	denotes	normal
T522	1904-1912	JJ	denotes	anterior
T523	1913-1919	NN	denotes	streak
T521	1920-1931	NN	denotes	development
T524	1931-1932	.	denotes	.
T525	1932-2121	sentence	denotes	This knowledge may prove to be important for understanding the mechanism by which the dominant polyglutamine expansion in huntingtin determines the loss of neurons in Huntington's disease.
T526	1933-1937	DT	denotes	This
T527	1938-1947	NN	denotes	knowledge
T529	1948-1951	MD	denotes	may
T528	1952-1957	VB	denotes	prove
T530	1958-1960	TO	denotes	to
T531	1961-1963	VB	denotes	be
T532	1964-1973	JJ	denotes	important
T533	1974-1977	IN	denotes	for
T534	1978-1991	VBG	denotes	understanding
T535	1992-1995	DT	denotes	the
T536	1996-2005	NN	denotes	mechanism
T537	2006-2008	IN	denotes	by
T539	2009-2014	WDT	denotes	which
T540	2015-2018	DT	denotes	the
T542	2019-2027	JJ	denotes	dominant
T543	2028-2041	NN	denotes	polyglutamine
T541	2042-2051	NN	denotes	expansion
T544	2052-2054	IN	denotes	in
T545	2055-2065	NN	denotes	huntingtin
T538	2066-2076	VBZ	denotes	determines
T546	2077-2080	DT	denotes	the
T547	2081-2085	NN	denotes	loss
T548	2086-2088	IN	denotes	of
T549	2089-2096	NNS	denotes	neurons
T550	2097-2099	IN	denotes	in
T551	2100-2110	NNP	denotes	Huntington
T553	2110-2112	POS	denotes	's
T552	2113-2120	NN	denotes	disease
T554	2120-2121	.	denotes	.
T927	2134-2144	NNP	denotes	Huntington
T929	2144-2146	POS	denotes	's
T928	2147-2154	NN	denotes	disease
T931	2155-2156	-LRB-	denotes	(
T932	2156-2158	NN	denotes	HD
T933	2158-2159	-RRB-	denotes	)
T930	2160-2162	VBZ	denotes	is
T934	2163-2164	DT	denotes	a
T936	2165-2175	RB	denotes	dominantly
T937	2176-2185	VBN	denotes	inherited
T938	2186-2203	JJ	denotes	neurodegenerative
T935	2204-2212	NN	denotes	disorder
T939	2213-2217	WDT	denotes	that
T941	2218-2220	VBZ	denotes	is
T940	2221-2227	VBN	denotes	caused
T942	2228-2230	IN	denotes	by
T943	2231-2234	NN	denotes	CAG
T944	2235-2242	NNS	denotes	repeats
T945	2243-2245	IN	denotes	in
T946	2246-2249	DT	denotes	the
T948	2250-2252	NN	denotes	HD
T947	2253-2258	NN	denotes	locus
T949	2259-2263	WDT	denotes	that
T950	2264-2270	VBP	denotes	extend
T951	2271-2272	DT	denotes	a
T953	2273-2286	NN	denotes	polyglutamine
T952	2287-2292	NN	denotes	tract
T954	2293-2295	IN	denotes	in
T955	2296-2297	DT	denotes	a
T957	2298-2308	JJ	denotes	ubiquitous
T958	2309-2313	NN	denotes	HEAT
T959	2314-2320	NN	denotes	domain
T956	2321-2328	NN	denotes	protein
T960	2329-2335	VBN	denotes	called
T961	2336-2346	NN	denotes	huntingtin
T962	2347-2348	-LRB-	denotes	[
T963	2348-2349	CD	denotes	1
T964	2349-2350	-RRB-	denotes	]
T965	2350-2351	.	denotes	.
T966	2351-2531	sentence	denotes	The molecular mechanism by which the new property that is conferred on huntingtin by the polyglutamine expansion leads to the hallmark loss of striatal neurons in HD is not known.
T967	2352-2355	DT	denotes	The
T969	2356-2365	JJ	denotes	molecular
T968	2366-2375	NN	denotes	mechanism
T971	2376-2378	IN	denotes	by
T973	2379-2384	WDT	denotes	which
T974	2385-2388	DT	denotes	the
T976	2389-2392	JJ	denotes	new
T975	2393-2401	NN	denotes	property
T977	2402-2406	WDT	denotes	that
T979	2407-2409	VBZ	denotes	is
T978	2410-2419	VBN	denotes	conferred
T980	2420-2422	IN	denotes	on
T981	2423-2433	NN	denotes	huntingtin
T982	2434-2436	IN	denotes	by
T983	2437-2440	DT	denotes	the
T985	2441-2454	NN	denotes	polyglutamine
T984	2455-2464	NN	denotes	expansion
T972	2465-2470	VBZ	denotes	leads
T986	2471-2473	IN	denotes	to
T987	2474-2477	DT	denotes	the
T989	2478-2486	NN	denotes	hallmark
T988	2487-2491	NN	denotes	loss
T990	2492-2494	IN	denotes	of
T991	2495-2503	JJ	denotes	striatal
T992	2504-2511	NNS	denotes	neurons
T993	2512-2514	IN	denotes	in
T994	2515-2517	NN	denotes	HD
T995	2518-2520	VBZ	denotes	is
T996	2521-2524	RB	denotes	not
T970	2525-2530	VBN	denotes	known
T997	2530-2531	.	denotes	.
T998	2531-2694	sentence	denotes	However, polyglutamine expansions in unrelated proteins that target distinct neuronal cell populations cause distinct 'polyglutamine' neurodegenerative disorders.
T999	2532-2539	RB	denotes	However
T1001	2539-2541	,	denotes	,
T1002	2541-2554	NN	denotes	polyglutamine
T1003	2555-2565	NNS	denotes	expansions
T1004	2566-2568	IN	denotes	in
T1005	2569-2578	JJ	denotes	unrelated
T1006	2579-2587	NN	denotes	proteins
T1007	2588-2592	WDT	denotes	that
T1008	2593-2599	VBP	denotes	target
T1009	2600-2608	JJ	denotes	distinct
T1011	2609-2617	JJ	denotes	neuronal
T1012	2618-2622	NN	denotes	cell
T1010	2623-2634	NNS	denotes	populations
T1000	2635-2640	VBP	denotes	cause
T1013	2641-2649	JJ	denotes	distinct
T1015	2650-2651	NN	denotes	'
T1016	2651-2664	NN	denotes	polyglutamine
T1017	2664-2665	''	denotes	'
T1018	2666-2683	JJ	denotes	neurodegenerative
T1014	2684-2693	NNS	denotes	disorders
T1019	2693-2694	.	denotes	.
T1020	2694-2925	sentence	denotes	This observation strongly suggests that the striatal cell specificity of the polyglutamine expansion in the context of huntingtin must be determined by some aspect of huntingtin's structure, subcellular location or activities [2].
T1021	2695-2699	DT	denotes	This
T1022	2700-2711	NN	denotes	observation
T1024	2712-2720	RB	denotes	strongly
T1023	2721-2729	VBZ	denotes	suggests
T1025	2730-2734	IN	denotes	that
T1027	2735-2738	DT	denotes	the
T1029	2739-2747	JJ	denotes	striatal
T1030	2748-2752	NN	denotes	cell
T1028	2753-2764	NN	denotes	specificity
T1031	2765-2767	IN	denotes	of
T1032	2768-2771	DT	denotes	the
T1034	2772-2785	NN	denotes	polyglutamine
T1033	2786-2795	NN	denotes	expansion
T1035	2796-2798	IN	denotes	in
T1036	2799-2802	DT	denotes	the
T1037	2803-2810	NN	denotes	context
T1038	2811-2813	IN	denotes	of
T1039	2814-2824	NN	denotes	huntingtin
T1040	2825-2829	MD	denotes	must
T1041	2830-2832	VB	denotes	be
T1026	2833-2843	VBN	denotes	determined
T1042	2844-2846	IN	denotes	by
T1043	2847-2851	DT	denotes	some
T1044	2852-2858	NN	denotes	aspect
T1045	2859-2861	IN	denotes	of
T1046	2862-2872	NN	denotes	huntingtin
T1048	2872-2874	POS	denotes	's
T1047	2875-2884	NN	denotes	structure
T1049	2884-2886	,	denotes	,
T1050	2886-2897	JJ	denotes	subcellular
T1051	2898-2906	NN	denotes	location
T1052	2907-2909	CC	denotes	or
T1053	2910-2920	NNS	denotes	activities
T1054	2921-2922	-LRB-	denotes	[
T1055	2922-2923	CD	denotes	2
T1056	2923-2924	-RRB-	denotes	]
T1057	2924-2925	.	denotes	.
T1058	2925-3111	sentence	denotes	Huntingtin is postulated to function as a flexible ~350 kDa HEAT domain scaffold that may facilitate the assembly and possibly the subcellular location of large protein complexes [3-7].
T1059	2926-2936	NN	denotes	Huntingtin
T1061	2937-2939	VBZ	denotes	is
T1060	2940-2950	VBN	denotes	postulated
T1062	2951-2953	TO	denotes	to
T1063	2954-2962	VB	denotes	function
T1064	2963-2965	IN	denotes	as
T1065	2966-2967	DT	denotes	a
T1067	2968-2976	JJ	denotes	flexible
T1068	2977-2978	SYM	denotes	~
T1069	2978-2981	CD	denotes	350
T1070	2982-2985	NN	denotes	kDa
T1071	2986-2990	NN	denotes	HEAT
T1072	2991-2997	NN	denotes	domain
T1066	2998-3006	NN	denotes	scaffold
T1073	3007-3011	WDT	denotes	that
T1075	3012-3015	MD	denotes	may
T1074	3016-3026	VB	denotes	facilitate
T1076	3027-3030	DT	denotes	the
T1077	3031-3039	NN	denotes	assembly
T1078	3040-3043	CC	denotes	and
T1079	3044-3052	RB	denotes	possibly
T1081	3053-3056	DT	denotes	the
T1082	3057-3068	JJ	denotes	subcellular
T1080	3069-3077	NN	denotes	location
T1083	3078-3080	IN	denotes	of
T1084	3081-3086	JJ	denotes	large
T1086	3087-3094	NN	denotes	protein
T1085	3095-3104	NNS	denotes	complexes
T1087	3105-3106	-LRB-	denotes	[
T1088	3106-3107	CD	denotes	3
T1089	3107-3108	SYM	denotes	-
T1090	3108-3109	CD	denotes	7
T1091	3109-3110	-RRB-	denotes	]
T1092	3110-3111	.	denotes	.
T1093	3111-3380	sentence	denotes	Huntingtin's large number of diverse cytoplasmic and nuclear protein binding partners strongly suggest that huntingtin may participate in a variety of cellular processes that range from trafficking of growth factor complexes to gene transcription (reviewed in [5,8,9].
T1094	3112-3122	NNP	denotes	Huntingtin
T1096	3122-3124	POS	denotes	's
T1097	3125-3130	JJ	denotes	large
T1095	3131-3137	NN	denotes	number
T1099	3138-3140	IN	denotes	of
T1100	3141-3148	JJ	denotes	diverse
T1102	3149-3160	JJ	denotes	cytoplasmic
T1103	3161-3164	CC	denotes	and
T1104	3165-3172	JJ	denotes	nuclear
T1105	3173-3180	NN	denotes	protein
T1106	3181-3188	NN	denotes	binding
T1101	3189-3197	NNS	denotes	partners
T1107	3198-3206	RB	denotes	strongly
T1098	3207-3214	VBP	denotes	suggest
T1108	3215-3219	IN	denotes	that
T1110	3220-3230	NN	denotes	huntingtin
T1111	3231-3234	MD	denotes	may
T1109	3235-3246	VB	denotes	participate
T1112	3247-3249	IN	denotes	in
T1113	3250-3251	DT	denotes	a
T1114	3252-3259	NN	denotes	variety
T1115	3260-3262	IN	denotes	of
T1116	3263-3271	JJ	denotes	cellular
T1117	3272-3281	NNS	denotes	processes
T1118	3282-3286	WDT	denotes	that
T1119	3287-3292	VBP	denotes	range
T1120	3293-3297	IN	denotes	from
T1121	3298-3309	NN	denotes	trafficking
T1122	3310-3312	IN	denotes	of
T1123	3313-3319	NN	denotes	growth
T1125	3320-3326	NN	denotes	factor
T1124	3327-3336	NNS	denotes	complexes
T1126	3337-3339	IN	denotes	to
T1127	3340-3344	NN	denotes	gene
T1128	3345-3358	NN	denotes	transcription
T1129	3359-3360	-LRB-	denotes	(
T1130	3360-3368	VBN	denotes	reviewed
T1131	3369-3371	IN	denotes	in
T1132	3372-3373	-LRB-	denotes	[
T1133	3373-3374	CD	denotes	5
T1135	3374-3375	,	denotes	,
T1136	3375-3376	CD	denotes	8
T1137	3376-3377	,	denotes	,
T1134	3377-3378	CD	denotes	9
T1138	3378-3379	-RRB-	denotes	]
T1139	3379-3380	.	denotes	.
T1140	3380-3617	sentence	denotes	However, despite the potential importance of huntingtin's normal function to our understanding of how the dominant polyglutamine mutation causes HD pathology, huntingtin's precise molecular and cellular activities have not been defined.
T1141	3381-3388	RB	denotes	However
T1143	3388-3390	,	denotes	,
T1144	3390-3397	IN	denotes	despite
T1145	3398-3401	DT	denotes	the
T1147	3402-3411	JJ	denotes	potential
T1146	3412-3422	NN	denotes	importance
T1148	3423-3425	IN	denotes	of
T1149	3426-3436	NN	denotes	huntingtin
T1151	3436-3438	POS	denotes	's
T1152	3439-3445	JJ	denotes	normal
T1150	3446-3454	NN	denotes	function
T1153	3455-3457	IN	denotes	to
T1154	3458-3461	PRP$	denotes	our
T1155	3462-3475	NN	denotes	understanding
T1156	3476-3478	IN	denotes	of
T1157	3479-3482	WRB	denotes	how
T1159	3483-3486	DT	denotes	the
T1161	3487-3495	JJ	denotes	dominant
T1162	3496-3509	NN	denotes	polyglutamine
T1160	3510-3518	NN	denotes	mutation
T1158	3519-3525	VBZ	denotes	causes
T1163	3526-3528	NN	denotes	HD
T1164	3529-3538	NN	denotes	pathology
T1165	3538-3540	,	denotes	,
T1166	3540-3550	NN	denotes	huntingtin
T1168	3550-3552	POS	denotes	's
T1169	3553-3560	JJ	denotes	precise
T1170	3561-3570	JJ	denotes	molecular
T1171	3571-3574	CC	denotes	and
T1172	3575-3583	JJ	denotes	cellular
T1167	3584-3594	NNS	denotes	activities
T1173	3595-3599	VBP	denotes	have
T1174	3600-3603	RB	denotes	not
T1175	3604-3608	VBN	denotes	been
T1142	3609-3616	VBN	denotes	defined
T1176	3616-3617	.	denotes	.
T1177	3617-3761	sentence	denotes	Therefore, we, and others, set out to discover huntingtin's essential activities by studying the effects of huntingtin deficiency in the mouse.
T1178	3618-3627	RB	denotes	Therefore
T1180	3627-3629	,	denotes	,
T1181	3629-3631	PRP	denotes	we
T1182	3631-3633	,	denotes	,
T1183	3633-3636	CC	denotes	and
T1184	3637-3643	NNS	denotes	others
T1185	3643-3645	,	denotes	,
T1179	3645-3648	VBN	denotes	set
T1186	3649-3652	RP	denotes	out
T1187	3653-3655	TO	denotes	to
T1188	3656-3664	VB	denotes	discover
T1189	3665-3675	NN	denotes	huntingtin
T1191	3675-3677	POS	denotes	's
T1192	3678-3687	JJ	denotes	essential
T1190	3688-3698	NNS	denotes	activities
T1193	3699-3701	IN	denotes	by
T1194	3702-3710	VBG	denotes	studying
T1195	3711-3714	DT	denotes	the
T1196	3715-3722	NNS	denotes	effects
T1197	3723-3725	IN	denotes	of
T1198	3726-3736	NN	denotes	huntingtin
T1199	3737-3747	NN	denotes	deficiency
T1200	3748-3750	IN	denotes	in
T1201	3751-3754	DT	denotes	the
T1202	3755-3760	NN	denotes	mouse
T1203	3760-3761	.	denotes	.
T1204	3761-3964	sentence	denotes	Inactivation of the mouse HD gene (Hdh) has shown that huntingtin is not required for cell viability, as evidenced by the survival of mouse embryonic stem cells and neurons that lack huntingtin [10-12].
T1205	3762-3774	NN	denotes	Inactivation
T1207	3775-3777	IN	denotes	of
T1208	3778-3781	DT	denotes	the
T1210	3782-3787	NN	denotes	mouse
T1211	3788-3790	NN	denotes	HD
T1209	3791-3795	NN	denotes	gene
T1212	3796-3797	-LRB-	denotes	(
T1213	3797-3800	NN	denotes	Hdh
T1214	3800-3801	-RRB-	denotes	)
T1215	3802-3805	VBZ	denotes	has
T1206	3806-3811	VBN	denotes	shown
T1216	3812-3816	IN	denotes	that
T1218	3817-3827	NN	denotes	huntingtin
T1219	3828-3830	VBZ	denotes	is
T1220	3831-3834	RB	denotes	not
T1217	3835-3843	VBN	denotes	required
T1221	3844-3847	IN	denotes	for
T1222	3848-3852	NN	denotes	cell
T1223	3853-3862	NN	denotes	viability
T1224	3862-3864	,	denotes	,
T1225	3864-3866	IN	denotes	as
T1226	3867-3876	VBN	denotes	evidenced
T1227	3877-3879	IN	denotes	by
T1228	3880-3883	DT	denotes	the
T1229	3884-3892	NN	denotes	survival
T1230	3893-3895	IN	denotes	of
T1231	3896-3901	NN	denotes	mouse
T1233	3902-3911	JJ	denotes	embryonic
T1234	3912-3916	NN	denotes	stem
T1232	3917-3922	NNS	denotes	cells
T1235	3923-3926	CC	denotes	and
T1236	3927-3934	NNS	denotes	neurons
T1237	3935-3939	WDT	denotes	that
T1238	3940-3944	VBP	denotes	lack
T1239	3945-3955	NN	denotes	huntingtin
T1240	3956-3957	-LRB-	denotes	[
T1241	3957-3959	CD	denotes	10
T1242	3959-3960	SYM	denotes	-
T1243	3960-3962	CD	denotes	12
T1244	3962-3963	-RRB-	denotes	]
T1245	3963-3964	.	denotes	.
T1246	3964-4078	sentence	denotes	However, huntingtin is needed at the level of the organism for proper mammalian embryonic development [10,13,14].
T1247	3965-3972	RB	denotes	However
T1249	3972-3974	,	denotes	,
T1250	3974-3984	NN	denotes	huntingtin
T1251	3985-3987	VBZ	denotes	is
T1248	3988-3994	VBN	denotes	needed
T1252	3995-3997	IN	denotes	at
T1253	3998-4001	DT	denotes	the
T1254	4002-4007	NN	denotes	level
T1255	4008-4010	IN	denotes	of
T1256	4011-4014	DT	denotes	the
T1257	4015-4023	NN	denotes	organism
T1258	4024-4027	IN	denotes	for
T1259	4028-4034	JJ	denotes	proper
T1261	4035-4044	JJ	denotes	mammalian
T1262	4045-4054	JJ	denotes	embryonic
T1260	4055-4066	NN	denotes	development
T1263	4067-4068	-LRB-	denotes	[
T1265	4068-4070	CD	denotes	10
T1266	4070-4071	,	denotes	,
T1267	4071-4073	CD	denotes	13
T1268	4073-4074	,	denotes	,
T1264	4074-4076	CD	denotes	14
T1269	4076-4077	-RRB-	denotes	]
T1270	4077-4078	.	denotes	.
T1271	4078-4266	sentence	denotes	Complete lack of huntingtin results in developmental arrest during gastrulation, while severe reduction of huntingtin levels results in abnormal neurogenesis and perinatal lethality [15].
T1272	4079-4087	JJ	denotes	Complete
T1273	4088-4092	NN	denotes	lack
T1275	4093-4095	IN	denotes	of
T1276	4096-4106	NN	denotes	huntingtin
T1274	4107-4114	VBZ	denotes	results
T1277	4115-4117	IN	denotes	in
T1278	4118-4131	JJ	denotes	developmental
T1279	4132-4138	NN	denotes	arrest
T1280	4139-4145	IN	denotes	during
T1281	4146-4158	NN	denotes	gastrulation
T1282	4158-4160	,	denotes	,
T1283	4160-4165	IN	denotes	while
T1285	4166-4172	JJ	denotes	severe
T1286	4173-4182	NN	denotes	reduction
T1287	4183-4185	IN	denotes	of
T1288	4186-4196	NN	denotes	huntingtin
T1289	4197-4203	NNS	denotes	levels
T1284	4204-4211	VBZ	denotes	results
T1290	4212-4214	IN	denotes	in
T1291	4215-4223	JJ	denotes	abnormal
T1292	4224-4236	NN	denotes	neurogenesis
T1293	4237-4240	CC	denotes	and
T1294	4241-4250	JJ	denotes	perinatal
T1295	4251-4260	NN	denotes	lethality
T1296	4261-4262	-LRB-	denotes	[
T1297	4262-4264	CD	denotes	15
T1298	4264-4265	-RRB-	denotes	]
T1299	4265-4266	.	denotes	.
T1300	4266-4431	sentence	denotes	Analysis of huntingtin deficient Hdhex4/5/Hdhex4/5 embryos reveals that homozygous inactivation of the mouse HD gene does not overtly affect development until E7.0.
T1301	4267-4275	NN	denotes	Analysis
T1303	4276-4278	IN	denotes	of
T1304	4279-4289	NN	denotes	huntingtin
T1305	4290-4299	JJ	denotes	deficient
T1307	4300-4306	NN	denotes	Hdhex4
T1308	4306-4307	HYPH	denotes	/
T1309	4307-4308	CD	denotes	5
T1310	4308-4309	HYPH	denotes	/
T1311	4309-4315	NN	denotes	Hdhex4
T1312	4315-4316	HYPH	denotes	/
T1313	4316-4317	CD	denotes	5
T1306	4318-4325	NNS	denotes	embryos
T1302	4326-4333	VBZ	denotes	reveals
T1314	4334-4338	IN	denotes	that
T1316	4339-4349	JJ	denotes	homozygous
T1317	4350-4362	NN	denotes	inactivation
T1318	4363-4365	IN	denotes	of
T1319	4366-4369	DT	denotes	the
T1321	4370-4375	NN	denotes	mouse
T1322	4376-4378	NN	denotes	HD
T1320	4379-4383	NN	denotes	gene
T1323	4384-4388	VBZ	denotes	does
T1324	4389-4392	RB	denotes	not
T1325	4393-4400	RB	denotes	overtly
T1315	4401-4407	VB	denotes	affect
T1326	4408-4419	NN	denotes	development
T1327	4420-4425	IN	denotes	until
T1328	4426-4430	NN	denotes	E7.0
T1329	4430-4431	.	denotes	.
T1330	4431-4554	sentence	denotes	By E7.5, mutant embryos exhibit a shortened primitive streak, reduced size and, by morphology, lack a node and head folds.
T1331	4432-4434	IN	denotes	By
T1333	4435-4439	NN	denotes	E7.5
T1334	4439-4441	,	denotes	,
T1335	4441-4447	NN	denotes	mutant
T1336	4448-4455	NNS	denotes	embryos
T1332	4456-4463	VBP	denotes	exhibit
T1337	4464-4465	DT	denotes	a
T1339	4466-4475	VBN	denotes	shortened
T1340	4476-4485	JJ	denotes	primitive
T1338	4486-4492	NN	denotes	streak
T1341	4492-4494	,	denotes	,
T1342	4494-4501	VBN	denotes	reduced
T1343	4502-4506	NN	denotes	size
T1344	4507-4510	CC	denotes	and
T1345	4510-4512	,	denotes	,
T1346	4512-4514	IN	denotes	by
T1348	4515-4525	NN	denotes	morphology
T1349	4525-4527	,	denotes	,
T1347	4527-4531	VBP	denotes	lack
T1350	4532-4533	DT	denotes	a
T1351	4534-4538	NN	denotes	node
T1352	4539-4542	CC	denotes	and
T1353	4543-4547	NN	denotes	head
T1354	4548-4553	NNS	denotes	folds
T1355	4553-4554	.	denotes	.
T1356	4554-4597	sentence	denotes	Mutants are rapidly resorbed by E8.0 [10].
T1357	4555-4562	NNS	denotes	Mutants
T1359	4563-4566	VBP	denotes	are
T1360	4567-4574	RB	denotes	rapidly
T1358	4575-4583	VBN	denotes	resorbed
T1361	4584-4586	IN	denotes	by
T1362	4587-4591	NN	denotes	E8.0
T1363	4592-4593	-LRB-	denotes	[
T1364	4593-4595	CD	denotes	10
T1365	4595-4596	-RRB-	denotes	]
T1366	4596-4597	.	denotes	.
T1367	4597-4863	sentence	denotes	Importantly, the expression of huntingtin only in extraembryonic tissues in chimeras rescues this gastrulation phenotype, suggesting that huntingtin is required only in cells of the extraembryonic lineage and acts in a cell non-autonomous manner at this stage [16].
T1368	4598-4609	RB	denotes	Importantly
T1370	4609-4611	,	denotes	,
T1371	4611-4614	DT	denotes	the
T1372	4615-4625	NN	denotes	expression
T1373	4626-4628	IN	denotes	of
T1374	4629-4639	NN	denotes	huntingtin
T1375	4640-4644	RB	denotes	only
T1376	4645-4647	IN	denotes	in
T1377	4648-4662	JJ	denotes	extraembryonic
T1378	4663-4670	NNS	denotes	tissues
T1379	4671-4673	IN	denotes	in
T1380	4674-4682	NNS	denotes	chimeras
T1369	4683-4690	VBZ	denotes	rescues
T1381	4691-4695	DT	denotes	this
T1383	4696-4708	NN	denotes	gastrulation
T1382	4709-4718	NN	denotes	phenotype
T1384	4718-4720	,	denotes	,
T1385	4720-4730	VBG	denotes	suggesting
T1386	4731-4735	IN	denotes	that
T1388	4736-4746	NN	denotes	huntingtin
T1389	4747-4749	VBZ	denotes	is
T1387	4750-4758	VBN	denotes	required
T1390	4759-4763	RB	denotes	only
T1391	4764-4766	IN	denotes	in
T1392	4767-4772	NNS	denotes	cells
T1393	4773-4775	IN	denotes	of
T1394	4776-4779	DT	denotes	the
T1396	4780-4794	JJ	denotes	extraembryonic
T1395	4795-4802	NN	denotes	lineage
T1397	4803-4806	CC	denotes	and
T1398	4807-4811	VBZ	denotes	acts
T1399	4812-4814	IN	denotes	in
T1400	4815-4816	DT	denotes	a
T1402	4817-4821	NN	denotes	cell
T1403	4822-4836	JJ	denotes	non-autonomous
T1401	4837-4843	NN	denotes	manner
T1404	4844-4846	IN	denotes	at
T1405	4847-4851	DT	denotes	this
T1406	4852-4857	NN	denotes	stage
T1407	4858-4859	-LRB-	denotes	[
T1408	4859-4861	CD	denotes	16
T1409	4861-4862	-RRB-	denotes	]
T1410	4862-4863	.	denotes	.
T1411	4863-5103	sentence	denotes	Extraembryonic tissues are essential for supplying nutrients and signals that direct anterior/posterior axis formation and patterning in the developing embryo (reviewed in [17]), implicating huntingtin in either or both of these processes.
T1412	4864-4878	JJ	denotes	Extraembryonic
T1413	4879-4886	NNS	denotes	tissues
T1414	4887-4890	VBP	denotes	are
T1415	4891-4900	JJ	denotes	essential
T1416	4901-4904	IN	denotes	for
T1417	4905-4914	VBG	denotes	supplying
T1418	4915-4924	NNS	denotes	nutrients
T1419	4925-4928	CC	denotes	and
T1420	4929-4936	NNS	denotes	signals
T1421	4937-4941	WDT	denotes	that
T1422	4942-4948	VBP	denotes	direct
T1423	4949-4957	JJ	denotes	anterior
T1425	4957-4958	HYPH	denotes	/
T1424	4958-4967	JJ	denotes	posterior
T1427	4968-4972	NN	denotes	axis
T1426	4973-4982	NN	denotes	formation
T1428	4983-4986	CC	denotes	and
T1429	4987-4997	NN	denotes	patterning
T1430	4998-5000	IN	denotes	in
T1431	5001-5004	DT	denotes	the
T1433	5005-5015	VBG	denotes	developing
T1432	5016-5022	NN	denotes	embryo
T1434	5023-5024	-LRB-	denotes	(
T1435	5024-5032	VBN	denotes	reviewed
T1436	5033-5035	IN	denotes	in
T1437	5036-5037	-LRB-	denotes	[
T1438	5037-5039	CD	denotes	17
T1439	5039-5040	-RRB-	denotes	]
T1440	5040-5041	-RRB-	denotes	)
T1441	5041-5043	,	denotes	,
T1442	5043-5054	VBG	denotes	implicating
T1443	5055-5065	NN	denotes	huntingtin
T1444	5066-5068	IN	denotes	in
T1445	5069-5075	DT	denotes	either
T1446	5076-5078	CC	denotes	or
T1447	5079-5083	DT	denotes	both
T1448	5084-5086	IN	denotes	of
T1449	5087-5092	DT	denotes	these
T1450	5093-5102	NNS	denotes	processes
T1451	5102-5103	.	denotes	.
T1452	5103-5186	sentence	denotes	Of these possibilities, the nutritive role has been more extensively investigated.
T1453	5104-5106	IN	denotes	Of
T1455	5107-5112	DT	denotes	these
T1456	5113-5126	NNS	denotes	possibilities
T1457	5126-5128	,	denotes	,
T1458	5128-5131	DT	denotes	the
T1460	5132-5141	JJ	denotes	nutritive
T1459	5142-5146	NN	denotes	role
T1461	5147-5150	VBZ	denotes	has
T1462	5151-5155	VBN	denotes	been
T1463	5156-5160	RBR	denotes	more
T1464	5161-5172	RB	denotes	extensively
T1454	5173-5185	VBN	denotes	investigated
T1465	5185-5186	.	denotes	.
T1466	5186-5493	sentence	denotes	However, huntingtin deficient embryos do not display obvious visceral endoderm defects, with the notable exception of compromised iron transport in later stage mutants, although iron uptake is undisturbed [16] and endocytosis is not impaired in huntingtin deficient embryos or embryonic stem cells [16,18].
T1467	5187-5194	RB	denotes	However
T1469	5194-5196	,	denotes	,
T1470	5196-5206	NN	denotes	huntingtin
T1471	5207-5216	JJ	denotes	deficient
T1472	5217-5224	NNS	denotes	embryos
T1473	5225-5227	VBP	denotes	do
T1474	5228-5231	RB	denotes	not
T1468	5232-5239	VB	denotes	display
T1475	5240-5247	JJ	denotes	obvious
T1477	5248-5256	JJ	denotes	visceral
T1478	5257-5265	NN	denotes	endoderm
T1476	5266-5273	NNS	denotes	defects
T1479	5273-5275	,	denotes	,
T1480	5275-5279	IN	denotes	with
T1481	5280-5283	DT	denotes	the
T1483	5284-5291	JJ	denotes	notable
T1482	5292-5301	NN	denotes	exception
T1484	5302-5304	IN	denotes	of
T1485	5305-5316	VBN	denotes	compromised
T1487	5317-5321	NN	denotes	iron
T1486	5322-5331	NN	denotes	transport
T1488	5332-5334	IN	denotes	in
T1489	5335-5340	JJ	denotes	later
T1491	5341-5346	NN	denotes	stage
T1490	5347-5354	NNS	denotes	mutants
T1492	5354-5356	,	denotes	,
T1493	5356-5364	IN	denotes	although
T1495	5365-5369	NN	denotes	iron
T1496	5370-5376	NN	denotes	uptake
T1494	5377-5379	VBZ	denotes	is
T1497	5380-5391	JJ	denotes	undisturbed
T1498	5392-5393	-LRB-	denotes	[
T1499	5393-5395	CD	denotes	16
T1500	5395-5396	-RRB-	denotes	]
T1501	5397-5400	CC	denotes	and
T1502	5401-5412	NN	denotes	endocytosis
T1504	5413-5415	VBZ	denotes	is
T1505	5416-5419	RB	denotes	not
T1503	5420-5428	VBN	denotes	impaired
T1506	5429-5431	IN	denotes	in
T1507	5432-5442	NN	denotes	huntingtin
T1508	5443-5452	JJ	denotes	deficient
T1509	5453-5460	NNS	denotes	embryos
T1510	5461-5463	CC	denotes	or
T1511	5464-5473	JJ	denotes	embryonic
T1513	5474-5478	NN	denotes	stem
T1512	5479-5484	NNS	denotes	cells
T1514	5485-5486	-LRB-	denotes	[
T1516	5486-5488	CD	denotes	16
T1517	5488-5489	,	denotes	,
T1515	5489-5491	CD	denotes	18
T1518	5491-5492	-RRB-	denotes	]
T1519	5492-5493	.	denotes	.
T1520	5493-5769	sentence	denotes	By the same token, huntingtin shuttles through the nucleus, where it is required for proper nuclear localization of its transcription factor partners, suggesting that huntingtin may play a role in transcription cascades in extraembryonic tissues that pattern the embryo [18].
T1521	5494-5496	IN	denotes	By
T1523	5497-5500	DT	denotes	the
T1525	5501-5505	JJ	denotes	same
T1524	5506-5511	NN	denotes	token
T1526	5511-5513	,	denotes	,
T1527	5513-5523	NN	denotes	huntingtin
T1522	5524-5532	VBZ	denotes	shuttles
T1528	5533-5540	IN	denotes	through
T1529	5541-5544	DT	denotes	the
T1530	5545-5552	NN	denotes	nucleus
T1531	5552-5554	,	denotes	,
T1532	5554-5559	WRB	denotes	where
T1534	5560-5562	PRP	denotes	it
T1535	5563-5565	VBZ	denotes	is
T1533	5566-5574	VBN	denotes	required
T1536	5575-5578	IN	denotes	for
T1537	5579-5585	JJ	denotes	proper
T1539	5586-5593	JJ	denotes	nuclear
T1538	5594-5606	NN	denotes	localization
T1540	5607-5609	IN	denotes	of
T1541	5610-5613	PRP$	denotes	its
T1543	5614-5627	NN	denotes	transcription
T1544	5628-5634	NN	denotes	factor
T1542	5635-5643	NNS	denotes	partners
T1545	5643-5645	,	denotes	,
T1546	5645-5655	VBG	denotes	suggesting
T1547	5656-5660	IN	denotes	that
T1549	5661-5671	NN	denotes	huntingtin
T1550	5672-5675	MD	denotes	may
T1548	5676-5680	VB	denotes	play
T1551	5681-5682	DT	denotes	a
T1552	5683-5687	NN	denotes	role
T1553	5688-5690	IN	denotes	in
T1554	5691-5704	NN	denotes	transcription
T1555	5705-5713	NNS	denotes	cascades
T1556	5714-5716	IN	denotes	in
T1557	5717-5731	JJ	denotes	extraembryonic
T1558	5732-5739	NNS	denotes	tissues
T1559	5740-5744	WDT	denotes	that
T1560	5745-5752	VBP	denotes	pattern
T1561	5753-5756	DT	denotes	the
T1562	5757-5763	NN	denotes	embryo
T1563	5764-5765	-LRB-	denotes	[
T1564	5765-5767	CD	denotes	18
T1565	5767-5768	-RRB-	denotes	]
T1566	5768-5769	.	denotes	.
T1567	5769-5964	sentence	denotes	Therefore, we have examined this hypothesis, by monitoring the expression of genes that determine normal embryonic patterning and morphogenesis in Hdhex4/5/Hdhex4/5 huntingtin deficient embryos.
T1568	5770-5779	RB	denotes	Therefore
T1570	5779-5781	,	denotes	,
T1571	5781-5783	PRP	denotes	we
T1572	5784-5788	VBP	denotes	have
T1569	5789-5797	VBN	denotes	examined
T1573	5798-5802	DT	denotes	this
T1574	5803-5813	NN	denotes	hypothesis
T1575	5813-5815	,	denotes	,
T1576	5815-5817	IN	denotes	by
T1577	5818-5828	VBG	denotes	monitoring
T1578	5829-5832	DT	denotes	the
T1579	5833-5843	NN	denotes	expression
T1580	5844-5846	IN	denotes	of
T1581	5847-5852	NNS	denotes	genes
T1582	5853-5857	WDT	denotes	that
T1583	5858-5867	VBP	denotes	determine
T1584	5868-5874	JJ	denotes	normal
T1586	5875-5884	JJ	denotes	embryonic
T1585	5885-5895	NN	denotes	patterning
T1587	5896-5899	CC	denotes	and
T1588	5900-5913	NN	denotes	morphogenesis
T1589	5914-5916	IN	denotes	in
T1590	5917-5923	NN	denotes	Hdhex4
T1592	5923-5924	HYPH	denotes	/
T1593	5924-5925	CD	denotes	5
T1594	5925-5926	HYPH	denotes	/
T1595	5926-5932	NN	denotes	Hdhex4
T1596	5932-5933	HYPH	denotes	/
T1597	5933-5934	CD	denotes	5
T1598	5935-5945	NN	denotes	huntingtin
T1599	5946-5955	JJ	denotes	deficient
T1591	5956-5963	NNS	denotes	embryos
T1600	5963-5964	.	denotes	.
T1601	5964-6184	sentence	denotes	Our results support and refine the hypothesis, indicating that huntingtin is required for proper mesoderm patterning and for normal regional restriction of the expression of a subset of growth and transcription factors.
T1602	5965-5968	PRP$	denotes	Our
T1603	5969-5976	NNS	denotes	results
T1604	5977-5984	VBP	denotes	support
T1605	5985-5988	CC	denotes	and
T1606	5989-5995	VBP	denotes	refine
T1607	5996-5999	DT	denotes	the
T1608	6000-6010	NN	denotes	hypothesis
T1609	6010-6012	,	denotes	,
T1610	6012-6022	VBG	denotes	indicating
T1611	6023-6027	IN	denotes	that
T1613	6028-6038	NN	denotes	huntingtin
T1614	6039-6041	VBZ	denotes	is
T1612	6042-6050	VBN	denotes	required
T1615	6051-6054	IN	denotes	for
T1616	6055-6061	JJ	denotes	proper
T1618	6062-6070	NN	denotes	mesoderm
T1617	6071-6081	NN	denotes	patterning
T1619	6082-6085	CC	denotes	and
T1620	6086-6089	IN	denotes	for
T1621	6090-6096	JJ	denotes	normal
T1623	6097-6105	JJ	denotes	regional
T1622	6106-6117	NN	denotes	restriction
T1624	6118-6120	IN	denotes	of
T1625	6121-6124	DT	denotes	the
T1626	6125-6135	NN	denotes	expression
T1627	6136-6138	IN	denotes	of
T1628	6139-6140	DT	denotes	a
T1629	6141-6147	NN	denotes	subset
T1630	6148-6150	IN	denotes	of
T1631	6151-6157	NN	denotes	growth
T1633	6158-6161	CC	denotes	and
T1634	6162-6175	NN	denotes	transcription
T1632	6176-6183	NNS	denotes	factors
T1635	6183-6184	.	denotes	.
T2201	6195-6205	NN	denotes	Huntingtin
T2203	6205-6206	HYPH	denotes	-
T2202	6206-6215	JJ	denotes	deficient
T2204	6216-6223	NNS	denotes	embryos
T2205	6224-6231	VBP	denotes	exhibit
T2206	6232-6240	JJ	denotes	abnormal
T2208	6241-6247	NN	denotes	streak
T2207	6248-6259	NN	denotes	progression
T2209	6260-6263	CC	denotes	and
T2210	6264-6272	JJ	denotes	paraxial
T2212	6273-6281	NN	denotes	mesoderm
T2211	6282-6292	NN	denotes	production
T2213	6292-6601	sentence	denotes	Since extraembryonic tissues supply nutrients to the developing embryo, we tested the possibility that huntingtin deficiency may perturb this function by performing RT-PCR analysis to examine the expression of a panel of 'nutritive' genes in E7.5 wild-type and Hdhex4/5/Hdhex4/5 huntingtin deficient embryos.
T2214	6293-6298	IN	denotes	Since
T2216	6299-6313	JJ	denotes	extraembryonic
T2217	6314-6321	NNS	denotes	tissues
T2215	6322-6328	VBP	denotes	supply
T2219	6329-6338	NNS	denotes	nutrients
T2220	6339-6341	IN	denotes	to
T2221	6342-6345	DT	denotes	the
T2223	6346-6356	VBG	denotes	developing
T2222	6357-6363	NN	denotes	embryo
T2224	6363-6365	,	denotes	,
T2225	6365-6367	PRP	denotes	we
T2218	6368-6374	VBD	denotes	tested
T2226	6375-6378	DT	denotes	the
T2227	6379-6390	NN	denotes	possibility
T2228	6391-6395	IN	denotes	that
T2230	6396-6406	NN	denotes	huntingtin
T2231	6407-6417	NN	denotes	deficiency
T2232	6418-6421	MD	denotes	may
T2229	6422-6429	VB	denotes	perturb
T2233	6430-6434	DT	denotes	this
T2234	6435-6443	NN	denotes	function
T2235	6444-6446	IN	denotes	by
T2236	6447-6457	VBG	denotes	performing
T2237	6458-6460	NN	denotes	RT
T2239	6460-6461	HYPH	denotes	-
T2238	6461-6464	NN	denotes	PCR
T2240	6465-6473	NN	denotes	analysis
T2241	6474-6476	TO	denotes	to
T2242	6477-6484	VB	denotes	examine
T2243	6485-6488	DT	denotes	the
T2244	6489-6499	NN	denotes	expression
T2245	6500-6502	IN	denotes	of
T2246	6503-6504	DT	denotes	a
T2247	6505-6510	NN	denotes	panel
T2248	6511-6513	IN	denotes	of
T2249	6514-6515	``	denotes	'
T2250	6515-6524	JJ	denotes	nutritive
T2252	6524-6525	''	denotes	'
T2251	6526-6531	NNS	denotes	genes
T2253	6532-6534	IN	denotes	in
T2254	6535-6539	NN	denotes	E7.5
T2256	6540-6544	JJ	denotes	wild
T2258	6544-6545	HYPH	denotes	-
T2257	6545-6549	NN	denotes	type
T2259	6550-6553	CC	denotes	and
T2260	6554-6560	NN	denotes	Hdhex4
T2261	6560-6561	HYPH	denotes	/
T2262	6561-6562	CD	denotes	5
T2263	6562-6563	HYPH	denotes	/
T2264	6563-6569	NN	denotes	Hdhex4
T2265	6569-6570	HYPH	denotes	/
T2266	6570-6571	CD	denotes	5
T2267	6572-6582	NN	denotes	huntingtin
T2268	6583-6592	JJ	denotes	deficient
T2255	6593-6600	NNS	denotes	embryos
T2269	6600-6601	.	denotes	.
T2270	6601-7026	sentence	denotes	Consistent with a previous report [16], no obvious differences were found in the expression of "nutritive" genes (Hnf4, Afp, Tfn, ApoAI, Apo-AIV, and ApoB) or genes involved in yolk sac hematopoiesis or vasculogenesis (Ttr, Rbp, Flt1, Flk1, Tal1, Rbtn2, GATA1) (data not shown), suggesting that huntingtin is not essential for the proper expression of genes required for the nutritive function of the extraembryonic tissues.
T2271	6602-6612	JJ	denotes	Consistent
T2273	6613-6617	IN	denotes	with
T2274	6618-6619	DT	denotes	a
T2276	6620-6628	JJ	denotes	previous
T2275	6629-6635	NN	denotes	report
T2277	6636-6637	-LRB-	denotes	[
T2278	6637-6639	CD	denotes	16
T2279	6639-6640	-RRB-	denotes	]
T2280	6640-6642	,	denotes	,
T2281	6642-6644	DT	denotes	no
T2283	6645-6652	JJ	denotes	obvious
T2282	6653-6664	NNS	denotes	differences
T2284	6665-6669	VBD	denotes	were
T2272	6670-6675	VBN	denotes	found
T2285	6676-6678	IN	denotes	in
T2286	6679-6682	DT	denotes	the
T2287	6683-6693	NN	denotes	expression
T2288	6694-6696	IN	denotes	of
T2289	6697-6698	``	denotes	"
T2290	6698-6707	JJ	denotes	nutritive
T2292	6707-6708	''	denotes	"
T2291	6709-6714	NNS	denotes	genes
T2293	6715-6716	-LRB-	denotes	(
T2294	6716-6720	NN	denotes	Hnf4
T2295	6720-6722	,	denotes	,
T2296	6722-6725	NN	denotes	Afp
T2297	6725-6727	,	denotes	,
T2298	6727-6730	NN	denotes	Tfn
T2299	6730-6732	,	denotes	,
T2300	6732-6737	NN	denotes	ApoAI
T2301	6737-6739	,	denotes	,
T2302	6739-6742	NN	denotes	Apo
T2304	6742-6743	HYPH	denotes	-
T2303	6743-6746	NN	denotes	AIV
T2305	6746-6748	,	denotes	,
T2306	6748-6751	CC	denotes	and
T2307	6752-6756	NN	denotes	ApoB
T2308	6756-6757	-RRB-	denotes	)
T2309	6758-6760	CC	denotes	or
T2310	6761-6766	NNS	denotes	genes
T2311	6767-6775	VBN	denotes	involved
T2312	6776-6778	IN	denotes	in
T2313	6779-6783	NN	denotes	yolk
T2314	6784-6787	NN	denotes	sac
T2315	6788-6801	NN	denotes	hematopoiesis
T2316	6802-6804	CC	denotes	or
T2317	6805-6819	NN	denotes	vasculogenesis
T2318	6820-6821	-LRB-	denotes	(
T2319	6821-6824	NN	denotes	Ttr
T2321	6824-6826	,	denotes	,
T2322	6826-6829	NN	denotes	Rbp
T2323	6829-6831	,	denotes	,
T2324	6831-6835	NN	denotes	Flt1
T2325	6835-6837	,	denotes	,
T2326	6837-6841	NN	denotes	Flk1
T2327	6841-6843	,	denotes	,
T2328	6843-6847	NN	denotes	Tal1
T2329	6847-6849	,	denotes	,
T2330	6849-6854	NN	denotes	Rbtn2
T2331	6854-6856	,	denotes	,
T2320	6856-6861	NN	denotes	GATA1
T2332	6861-6862	-RRB-	denotes	)
T2333	6863-6864	-LRB-	denotes	(
T2335	6864-6868	NNS	denotes	data
T2336	6869-6872	RB	denotes	not
T2334	6873-6878	VBN	denotes	shown
T2337	6878-6879	-RRB-	denotes	)
T2338	6879-6881	,	denotes	,
T2339	6881-6891	VBG	denotes	suggesting
T2340	6892-6896	IN	denotes	that
T2342	6897-6907	NN	denotes	huntingtin
T2341	6908-6910	VBZ	denotes	is
T2343	6911-6914	RB	denotes	not
T2344	6915-6924	JJ	denotes	essential
T2345	6925-6928	IN	denotes	for
T2346	6929-6932	DT	denotes	the
T2348	6933-6939	JJ	denotes	proper
T2347	6940-6950	NN	denotes	expression
T2349	6951-6953	IN	denotes	of
T2350	6954-6959	NNS	denotes	genes
T2351	6960-6968	VBN	denotes	required
T2352	6969-6972	IN	denotes	for
T2353	6973-6976	DT	denotes	the
T2355	6977-6986	JJ	denotes	nutritive
T2354	6987-6995	NN	denotes	function
T2356	6996-6998	IN	denotes	of
T2357	6999-7002	DT	denotes	the
T2359	7003-7017	JJ	denotes	extraembryonic
T2358	7018-7025	NNS	denotes	tissues
T2360	7025-7026	.	denotes	.
T2361	7026-7275	sentence	denotes	To investigate huntingtin's developmental activities, we then analyzed the expression of genes which pattern the early embryo or mark morphogenic landmarks in wild-type and Hdhex4/5/Hdhex4/5 embryos by whole mount and section in situ hybridization.
T2362	7027-7029	TO	denotes	To
T2363	7030-7041	VB	denotes	investigate
T2365	7042-7052	NN	denotes	huntingtin
T2367	7052-7054	POS	denotes	's
T2368	7055-7068	JJ	denotes	developmental
T2366	7069-7079	NNS	denotes	activities
T2369	7079-7081	,	denotes	,
T2370	7081-7083	PRP	denotes	we
T2371	7084-7088	RB	denotes	then
T2364	7089-7097	VBD	denotes	analyzed
T2372	7098-7101	DT	denotes	the
T2373	7102-7112	NN	denotes	expression
T2374	7113-7115	IN	denotes	of
T2375	7116-7121	NNS	denotes	genes
T2376	7122-7127	WDT	denotes	which
T2377	7128-7135	VBP	denotes	pattern
T2378	7136-7139	DT	denotes	the
T2380	7140-7145	JJ	denotes	early
T2379	7146-7152	NN	denotes	embryo
T2381	7153-7155	CC	denotes	or
T2382	7156-7160	VBP	denotes	mark
T2383	7161-7172	JJ	denotes	morphogenic
T2384	7173-7182	NNS	denotes	landmarks
T2385	7183-7185	IN	denotes	in
T2386	7186-7190	JJ	denotes	wild
T2388	7190-7191	HYPH	denotes	-
T2387	7191-7195	NN	denotes	type
T2390	7196-7199	CC	denotes	and
T2391	7200-7206	NN	denotes	Hdhex4
T2392	7206-7207	HYPH	denotes	/
T2393	7207-7208	CD	denotes	5
T2394	7208-7209	HYPH	denotes	/
T2395	7209-7215	NN	denotes	Hdhex4
T2396	7215-7216	HYPH	denotes	/
T2397	7216-7217	CD	denotes	5
T2389	7218-7225	NNS	denotes	embryos
T2398	7226-7228	IN	denotes	by
T2399	7229-7234	JJ	denotes	whole
T2400	7235-7240	NN	denotes	mount
T2402	7241-7244	CC	denotes	and
T2403	7245-7252	NN	denotes	section
T2404	7253-7255	FW	denotes	in
T2405	7256-7260	FW	denotes	situ
T2401	7261-7274	NN	denotes	hybridization
T2406	7274-7275	.	denotes	.
T2407	7275-7500	sentence	denotes	The dissections confirmed previous morphologic data at E7.0–7.5 that all Hdhex4/5/Hdhex4/5 homozygotes exhibit abnormal morphology, including shortened primitive streak and a lack of morphological head folds or node [10,13].
T2408	7276-7279	DT	denotes	The
T2409	7280-7291	NNS	denotes	dissections
T2410	7292-7301	VBD	denotes	confirmed
T2411	7302-7310	JJ	denotes	previous
T2413	7311-7322	JJ	denotes	morphologic
T2412	7323-7327	NNS	denotes	data
T2414	7328-7330	IN	denotes	at
T2415	7331-7335	NN	denotes	E7.0
T2416	7335-7336	SYM	denotes	–
T2417	7336-7339	NN	denotes	7.5
T2418	7340-7344	IN	denotes	that
T2420	7345-7348	DT	denotes	all
T2422	7349-7355	NN	denotes	Hdhex4
T2423	7355-7356	HYPH	denotes	/
T2424	7356-7357	CD	denotes	5
T2425	7357-7358	HYPH	denotes	/
T2426	7358-7364	NN	denotes	Hdhex4
T2427	7364-7365	HYPH	denotes	/
T2428	7365-7366	CD	denotes	5
T2421	7367-7378	NNS	denotes	homozygotes
T2419	7379-7386	VBP	denotes	exhibit
T2429	7387-7395	JJ	denotes	abnormal
T2430	7396-7406	NN	denotes	morphology
T2431	7406-7408	,	denotes	,
T2432	7408-7417	VBG	denotes	including
T2433	7418-7427	VBN	denotes	shortened
T2435	7428-7437	JJ	denotes	primitive
T2434	7438-7444	NN	denotes	streak
T2436	7445-7448	CC	denotes	and
T2437	7449-7450	DT	denotes	a
T2438	7451-7455	NN	denotes	lack
T2439	7456-7458	IN	denotes	of
T2440	7459-7472	JJ	denotes	morphological
T2442	7473-7477	NN	denotes	head
T2441	7478-7483	NNS	denotes	folds
T2443	7484-7486	CC	denotes	or
T2444	7487-7491	NN	denotes	node
T2445	7492-7493	-LRB-	denotes	[
T2447	7493-7495	CD	denotes	10
T2448	7495-7496	,	denotes	,
T2446	7496-7498	CD	denotes	13
T2449	7498-7499	-RRB-	denotes	]
T2450	7499-7500	.	denotes	.
T2451	7500-7658	sentence	denotes	The results of in situ hybridization analysis also confirmed that all three germ layers and extraembryonic tissue are formed in huntingtin deficient embryos.
T2452	7501-7504	DT	denotes	The
T2453	7505-7512	NNS	denotes	results
T2455	7513-7515	IN	denotes	of
T2456	7516-7518	FW	denotes	in
T2457	7519-7523	FW	denotes	situ
T2459	7524-7537	NN	denotes	hybridization
T2458	7538-7546	NN	denotes	analysis
T2460	7547-7551	RB	denotes	also
T2454	7552-7561	VBD	denotes	confirmed
T2461	7562-7566	IN	denotes	that
T2463	7567-7570	DT	denotes	all
T2465	7571-7576	CD	denotes	three
T2466	7577-7581	NN	denotes	germ
T2464	7582-7588	NNS	denotes	layers
T2467	7589-7592	CC	denotes	and
T2468	7593-7607	JJ	denotes	extraembryonic
T2469	7608-7614	NN	denotes	tissue
T2470	7615-7618	VBP	denotes	are
T2462	7619-7625	VBN	denotes	formed
T2471	7626-7628	IN	denotes	in
T2472	7629-7639	NN	denotes	huntingtin
T2473	7640-7649	JJ	denotes	deficient
T2474	7650-7657	NNS	denotes	embryos
T2475	7657-7658	.	denotes	.
T2476	7658-7849	sentence	denotes	Otx2, normally expressed in the anterior neuroectoderm and anterior visceral endoderm [19], is expressed in mutant embryos at E7.5 (Fig. 1A,B) although the expression domain appears reduced.
T2477	7659-7663	NN	denotes	Otx2
T2479	7663-7665	,	denotes	,
T2480	7665-7673	RB	denotes	normally
T2481	7674-7683	VBN	denotes	expressed
T2482	7684-7686	IN	denotes	in
T2483	7687-7690	DT	denotes	the
T2485	7691-7699	JJ	denotes	anterior
T2484	7700-7713	NN	denotes	neuroectoderm
T2486	7714-7717	CC	denotes	and
T2487	7718-7726	JJ	denotes	anterior
T2489	7727-7735	JJ	denotes	visceral
T2488	7736-7744	NN	denotes	endoderm
T2490	7745-7746	-LRB-	denotes	[
T2491	7746-7748	CD	denotes	19
T2492	7748-7749	-RRB-	denotes	]
T2493	7749-7751	,	denotes	,
T2494	7751-7753	VBZ	denotes	is
T2478	7754-7763	VBN	denotes	expressed
T2495	7764-7766	IN	denotes	in
T2496	7767-7773	NN	denotes	mutant
T2497	7774-7781	NNS	denotes	embryos
T2498	7782-7784	IN	denotes	at
T2499	7785-7789	NN	denotes	E7.5
T2500	7790-7791	-LRB-	denotes	(
T2502	7791-7795	NN	denotes	Fig.
T2503	7796-7798	NN	denotes	1A
T2504	7798-7799	,	denotes	,
T2501	7799-7800	NN	denotes	B
T2505	7800-7801	-RRB-	denotes	)
T2506	7802-7810	IN	denotes	although
T2508	7811-7814	DT	denotes	the
T2510	7815-7825	NN	denotes	expression
T2509	7826-7832	NN	denotes	domain
T2507	7833-7840	VBZ	denotes	appears
T2511	7841-7848	VBN	denotes	reduced
T2512	7848-7849	.	denotes	.
T2513	7849-8041	sentence	denotes	Similarly, Hesx1 expression is grossly normal in mutant embryos, with expression localized to the AVE and neuroectoderm (Fig. 1C–F, [20]), although the expression domain also appears reduced.
T2514	7850-7859	RB	denotes	Similarly
T2516	7859-7861	,	denotes	,
T2517	7861-7866	NN	denotes	Hesx1
T2518	7867-7877	NN	denotes	expression
T2515	7878-7880	VBZ	denotes	is
T2519	7881-7888	RB	denotes	grossly
T2520	7889-7895	JJ	denotes	normal
T2521	7896-7898	IN	denotes	in
T2522	7899-7905	NN	denotes	mutant
T2523	7906-7913	NNS	denotes	embryos
T2524	7913-7915	,	denotes	,
T2525	7915-7919	IN	denotes	with
T2527	7920-7930	NN	denotes	expression
T2526	7931-7940	VBN	denotes	localized
T2528	7941-7943	IN	denotes	to
T2529	7944-7947	DT	denotes	the
T2530	7948-7951	NN	denotes	AVE
T2531	7952-7955	CC	denotes	and
T2532	7956-7969	NN	denotes	neuroectoderm
T2533	7970-7971	-LRB-	denotes	(
T2535	7971-7975	NN	denotes	Fig.
T2536	7976-7978	CD	denotes	1C
T2537	7978-7979	SYM	denotes	–
T2538	7979-7980	NN	denotes	F
T2539	7980-7982	,	denotes	,
T2540	7982-7983	-LRB-	denotes	[
T2534	7983-7985	CD	denotes	20
T2541	7985-7986	-RRB-	denotes	]
T2542	7986-7987	-RRB-	denotes	)
T2543	7987-7989	,	denotes	,
T2544	7989-7997	IN	denotes	although
T2546	7998-8001	DT	denotes	the
T2548	8002-8012	NN	denotes	expression
T2547	8013-8019	NN	denotes	domain
T2549	8020-8024	RB	denotes	also
T2545	8025-8032	VBZ	denotes	appears
T2550	8033-8040	JJ	denotes	reduced
T2551	8040-8041	.	denotes	.
T2552	8041-8230	sentence	denotes	These results indicate appropriate specification and movement of anterior visceral endoderm (AVE) cells from the distal tip and suggest that neuroectoderm is induced in the mutant embryos.
T2553	8042-8047	DT	denotes	These
T2554	8048-8055	NNS	denotes	results
T2555	8056-8064	VBP	denotes	indicate
T2556	8065-8076	JJ	denotes	appropriate
T2557	8077-8090	NN	denotes	specification
T2558	8091-8094	CC	denotes	and
T2559	8095-8103	NN	denotes	movement
T2560	8104-8106	IN	denotes	of
T2561	8107-8115	JJ	denotes	anterior
T2563	8116-8124	JJ	denotes	visceral
T2562	8125-8133	NN	denotes	endoderm
T2565	8134-8135	-LRB-	denotes	(
T2566	8135-8138	NN	denotes	AVE
T2567	8138-8139	-RRB-	denotes	)
T2564	8140-8145	NNS	denotes	cells
T2568	8146-8150	IN	denotes	from
T2569	8151-8154	DT	denotes	the
T2571	8155-8161	JJ	denotes	distal
T2570	8162-8165	NN	denotes	tip
T2572	8166-8169	CC	denotes	and
T2573	8170-8177	VBP	denotes	suggest
T2574	8178-8182	IN	denotes	that
T2576	8183-8196	NN	denotes	neuroectoderm
T2577	8197-8199	VBZ	denotes	is
T2575	8200-8207	VBN	denotes	induced
T2578	8208-8210	IN	denotes	in
T2579	8211-8214	DT	denotes	the
T2581	8215-8221	NN	denotes	mutant
T2580	8222-8229	NNS	denotes	embryos
T2582	8229-8230	.	denotes	.
T2583	8230-8231	sentence	denotes
T8416	8241-8244	NN	denotes	AVE
T8417	8245-8257	NN	denotes	displacement
T8419	8258-8261	CC	denotes	and
T8420	8262-8270	JJ	denotes	anterior
T8422	8271-8283	NN	denotes	neurectoderm
T8421	8284-8293	NN	denotes	induction
T8418	8294-8299	VBP	denotes	occur
T8423	8300-8308	RB	denotes	normally
T8424	8309-8311	IN	denotes	in
T8425	8312-8315	DT	denotes	the
T8426	8316-8323	NN	denotes	absence
T8427	8324-8326	IN	denotes	of
T8428	8327-8337	NN	denotes	huntingtin
T8429	8337-8338	.	denotes	.
T8430	8338-8631	sentence	denotes	Whole mount in situ hybridization analysis of Otx2 (A,B) and Hesx (C-F) in E7.5 normal (A,C,E) and mutant (B,D,F) embryos reveals that neuroectoderm and anterior visceral endoderm (AVE) develop normally in huntingtin deficient embryos, although the neuroectoderm expression domain is reduced.
T8431	8339-8344	JJ	denotes	Whole
T8432	8345-8350	NN	denotes	mount
T8434	8351-8353	FW	denotes	in
T8435	8354-8358	FW	denotes	situ
T8436	8359-8372	NN	denotes	hybridization
T8433	8373-8381	NN	denotes	analysis
T8438	8382-8384	IN	denotes	of
T8439	8385-8389	NN	denotes	Otx2
T8440	8390-8391	-LRB-	denotes	(
T8442	8391-8392	NN	denotes	A
T8443	8392-8393	,	denotes	,
T8441	8393-8394	NN	denotes	B
T8444	8394-8395	-RRB-	denotes	)
T8445	8396-8399	CC	denotes	and
T8446	8400-8404	NN	denotes	Hesx
T8447	8405-8406	-LRB-	denotes	(
T8448	8406-8407	NN	denotes	C
T8449	8407-8408	SYM	denotes	-
T8450	8408-8409	NN	denotes	F
T8451	8409-8410	-RRB-	denotes	)
T8452	8411-8413	IN	denotes	in
T8453	8414-8418	NN	denotes	E7.5
T8455	8419-8425	JJ	denotes	normal
T8456	8426-8427	-LRB-	denotes	(
T8458	8427-8428	NN	denotes	A
T8459	8428-8429	,	denotes	,
T8460	8429-8430	NN	denotes	C
T8461	8430-8431	,	denotes	,
T8457	8431-8432	NN	denotes	E
T8462	8432-8433	-RRB-	denotes	)
T8463	8434-8437	CC	denotes	and
T8464	8438-8444	NN	denotes	mutant
T8465	8445-8446	-LRB-	denotes	(
T8467	8446-8447	NN	denotes	B
T8468	8447-8448	,	denotes	,
T8469	8448-8449	NN	denotes	D
T8470	8449-8450	,	denotes	,
T8466	8450-8451	NN	denotes	F
T8471	8451-8452	-RRB-	denotes	)
T8454	8453-8460	NNS	denotes	embryos
T8437	8461-8468	VBZ	denotes	reveals
T8472	8469-8473	IN	denotes	that
T8474	8474-8487	NN	denotes	neuroectoderm
T8475	8488-8491	CC	denotes	and
T8476	8492-8500	JJ	denotes	anterior
T8478	8501-8509	JJ	denotes	visceral
T8477	8510-8518	NN	denotes	endoderm
T8479	8519-8520	-LRB-	denotes	(
T8480	8520-8523	NN	denotes	AVE
T8481	8523-8524	-RRB-	denotes	)
T8473	8525-8532	VBP	denotes	develop
T8482	8533-8541	RB	denotes	normally
T8483	8542-8544	IN	denotes	in
T8484	8545-8555	NN	denotes	huntingtin
T8485	8556-8565	JJ	denotes	deficient
T8486	8566-8573	NNS	denotes	embryos
T8487	8573-8575	,	denotes	,
T8488	8575-8583	IN	denotes	although
T8490	8584-8587	DT	denotes	the
T8492	8588-8601	NN	denotes	neuroectoderm
T8493	8602-8612	NN	denotes	expression
T8491	8613-8619	NN	denotes	domain
T8494	8620-8622	VBZ	denotes	is
T8489	8623-8630	VBN	denotes	reduced
T8495	8630-8631	.	denotes	.
T8496	8631-8749	sentence	denotes	Asymmetrical expression of Hesx in mutant embryos (F) suggests that left-right transcriptional control is maintained.
T8497	8632-8644	JJ	denotes	Asymmetrical
T8498	8645-8655	NN	denotes	expression
T8500	8656-8658	IN	denotes	of
T8501	8659-8663	NN	denotes	Hesx
T8502	8664-8666	IN	denotes	in
T8503	8667-8673	NN	denotes	mutant
T8504	8674-8681	NNS	denotes	embryos
T8505	8682-8683	-LRB-	denotes	(
T8506	8683-8684	NN	denotes	F
T8507	8684-8685	-RRB-	denotes	)
T8499	8686-8694	VBZ	denotes	suggests
T8508	8695-8699	IN	denotes	that
T8510	8700-8704	JJ	denotes	left
T8512	8704-8705	SYM	denotes	-
T8511	8705-8710	JJ	denotes	right
T8514	8711-8726	JJ	denotes	transcriptional
T8513	8727-8734	NN	denotes	control
T8515	8735-8737	VBZ	denotes	is
T8509	8738-8748	VBN	denotes	maintained
T8516	8748-8749	.	denotes	.
T8517	8749-8900	sentence	denotes	Hnf3β expression in the definitive endoderm extends around the distal tip and is reduced in the AVE (*) in both normal (G,I) and mutant embryos (H,J).
T8518	8750-8755	NN	denotes	Hnf3β
T8519	8756-8766	NN	denotes	expression
T8521	8767-8769	IN	denotes	in
T8522	8770-8773	DT	denotes	the
T8524	8774-8784	JJ	denotes	definitive
T8523	8785-8793	NN	denotes	endoderm
T8520	8794-8801	VBZ	denotes	extends
T8525	8802-8808	IN	denotes	around
T8526	8809-8812	DT	denotes	the
T8528	8813-8819	JJ	denotes	distal
T8527	8820-8823	NN	denotes	tip
T8529	8824-8827	CC	denotes	and
T8530	8828-8830	VBZ	denotes	is
T8531	8831-8838	VBN	denotes	reduced
T8532	8839-8841	IN	denotes	in
T8533	8842-8845	DT	denotes	the
T8534	8846-8849	NN	denotes	AVE
T8535	8850-8851	-LRB-	denotes	(
T8536	8851-8852	SYM	denotes	*
T8537	8852-8853	-RRB-	denotes	)
T8538	8854-8856	IN	denotes	in
T8539	8857-8861	CC	denotes	both
T8541	8862-8868	JJ	denotes	normal
T8542	8869-8870	-LRB-	denotes	(
T8544	8870-8871	NN	denotes	G
T8545	8871-8872	,	denotes	,
T8543	8872-8873	NN	denotes	I
T8546	8873-8874	-RRB-	denotes	)
T8547	8875-8878	CC	denotes	and
T8548	8879-8885	NN	denotes	mutant
T8540	8886-8893	NNS	denotes	embryos
T8549	8894-8895	-LRB-	denotes	(
T8551	8895-8896	NN	denotes	H
T8552	8896-8897	,	denotes	,
T8550	8897-8898	NN	denotes	J
T8553	8898-8899	-RRB-	denotes	)
T8554	8899-8900	.	denotes	.
T8555	8900-9024	sentence	denotes	Taken together, these results suggest normal ectoderm and endoderm induction and localization in Hdhex4/5/Hdhex4/5 embryos.
T8556	8901-8906	VBN	denotes	Taken
T8558	8907-8915	RB	denotes	together
T8559	8915-8917	,	denotes	,
T8560	8917-8922	DT	denotes	these
T8561	8923-8930	NNS	denotes	results
T8557	8931-8938	VBP	denotes	suggest
T8562	8939-8945	JJ	denotes	normal
T8563	8946-8954	NN	denotes	ectoderm
T8564	8955-8958	CC	denotes	and
T8565	8959-8967	NN	denotes	endoderm
T8566	8968-8977	NN	denotes	induction
T8567	8978-8981	CC	denotes	and
T8568	8982-8994	NN	denotes	localization
T8569	8995-8997	IN	denotes	in
T8570	8998-9004	NN	denotes	Hdhex4
T8572	9004-9005	HYPH	denotes	/
T8573	9005-9006	CD	denotes	5
T8574	9006-9007	HYPH	denotes	/
T8575	9007-9013	NN	denotes	Hdhex4
T8576	9013-9014	HYPH	denotes	/
T8577	9014-9015	CD	denotes	5
T8571	9016-9023	NNS	denotes	embryos
T8578	9023-9024	.	denotes	.
T8579	9024-9133	sentence	denotes	Embryos are shown in lateral views, with anterior to the left in all pictures with the exception of E and F.
T8580	9025-9032	NNS	denotes	Embryos
T8582	9033-9036	VBP	denotes	are
T8581	9037-9042	VBN	denotes	shown
T8583	9043-9045	IN	denotes	in
T8584	9046-9053	JJ	denotes	lateral
T8585	9054-9059	NNS	denotes	views
T8586	9059-9061	,	denotes	,
T8587	9061-9065	IN	denotes	with
T8589	9066-9074	JJ	denotes	anterior
T8588	9075-9077	IN	denotes	to
T8590	9078-9081	DT	denotes	the
T8591	9082-9086	NN	denotes	left
T8592	9087-9089	IN	denotes	in
T8593	9090-9093	DT	denotes	all
T8594	9094-9102	NNS	denotes	pictures
T8595	9103-9107	IN	denotes	with
T8596	9108-9111	DT	denotes	the
T8597	9112-9121	NN	denotes	exception
T8598	9122-9124	IN	denotes	of
T8599	9125-9126	NN	denotes	E
T8600	9127-9130	CC	denotes	and
T8601	9131-9132	NN	denotes	F
T8602	9132-9133	.	denotes	.
T8603	9133-9189	sentence	denotes	Embryos are viewed from the anterior aspect in E and F.
T8604	9134-9141	NNS	denotes	Embryos
T8606	9142-9145	VBP	denotes	are
T8605	9146-9152	VBN	denotes	viewed
T8607	9153-9157	IN	denotes	from
T8608	9158-9161	DT	denotes	the
T8610	9162-9170	JJ	denotes	anterior
T8609	9171-9177	NN	denotes	aspect
T8611	9178-9180	IN	denotes	in
T8612	9181-9182	NN	denotes	E
T8613	9183-9186	CC	denotes	and
T8614	9187-9188	NN	denotes	F
T8615	9188-9189	.	denotes	.
T2585	9190-9191	VBN	denotes	T
T2584	9190-9195	sentence	denotes	To ex
T2586	9191-9192	RB	denotes	o
T2587	9192-9193	,	denotes
T2588	9193-9194	DT	denotes	e
T2589	9194-9195	NN	denotes	x
T2590	9195-9196	sentence	denotes	a
T2591	9195-9196	VBP	denotes	a
T2593	9196-9197	NNS	denotes	m
T2592	9196-9202	sentence	denotes	mine d
T2594	9197-9198	VBN	denotes	i
T2595	9198-9200	JJ	denotes	ne
T2596	9201-9202	CC	denotes	d
T2598	9202-9203	VB	denotes	e
T2597	9202-9309	sentence	denotes	efinitive endoderm formation, the expression of Hnf3β (FoxA2) in mutant and wild-type embryos was analyzed.
T2600	9203-9211	JJ	denotes	finitive
T2602	9212-9220	NN	denotes	endoderm
T2601	9221-9230	NN	denotes	formation
T2603	9230-9232	,	denotes	,
T2604	9232-9235	DT	denotes	the
T2605	9236-9246	NN	denotes	expression
T2606	9247-9249	IN	denotes	of
T2607	9250-9255	NN	denotes	Hnf3β
T2608	9256-9257	-LRB-	denotes	(
T2609	9257-9262	NN	denotes	FoxA2
T2610	9262-9263	-RRB-	denotes	)
T2611	9264-9266	IN	denotes	in
T2612	9267-9273	NN	denotes	mutant
T2614	9274-9277	CC	denotes	and
T2615	9278-9282	JJ	denotes	wild
T2617	9282-9283	HYPH	denotes	-
T2616	9283-9287	NN	denotes	type
T2613	9288-9295	NNS	denotes	embryos
T2618	9296-9299	VBD	denotes	was
T2599	9300-9308	VBN	denotes	analyzed
T2619	9308-9309	.	denotes	.
T2620	9309-9422	sentence	denotes	In wild-type embryos, Hnf3β expression is confined to the node and anterior definitive endoderm (Fig. 1G,I[21]).
T2621	9310-9312	IN	denotes	In
T2623	9313-9317	JJ	denotes	wild
T2625	9317-9318	HYPH	denotes	-
T2624	9318-9322	NN	denotes	type
T2626	9323-9330	NNS	denotes	embryos
T2627	9330-9332	,	denotes	,
T2628	9332-9337	NN	denotes	Hnf3β
T2629	9338-9348	NN	denotes	expression
T2630	9349-9351	VBZ	denotes	is
T2622	9352-9360	VBN	denotes	confined
T2631	9361-9363	IN	denotes	to
T2632	9364-9367	DT	denotes	the
T2633	9368-9372	NN	denotes	node
T2634	9373-9376	CC	denotes	and
T2635	9377-9385	JJ	denotes	anterior
T2637	9386-9396	JJ	denotes	definitive
T2636	9397-9405	NN	denotes	endoderm
T2638	9406-9407	-LRB-	denotes	(
T2640	9407-9411	NN	denotes	Fig.
T2642	9412-9414	NN	denotes	1G
T2643	9414-9415	,	denotes	,
T2641	9415-9416	NN	denotes	I
T2644	9416-9417	-LRB-	denotes	[
T2639	9417-9419	CD	denotes	21
T2645	9419-9420	-RRB-	denotes	]
T2646	9420-9421	-RRB-	denotes	)
T2647	9421-9422	.	denotes	.
T2648	9422-9585	sentence	denotes	Mutant embryos exhibit Hnf3β-reactive definitive endoderm over the disorganized anterior streak region and proceeding rostrally around the distal tip (Fig. 1H,J).
T2649	9423-9429	NN	denotes	Mutant
T2650	9430-9437	NNS	denotes	embryos
T2651	9438-9445	VBP	denotes	exhibit
T2652	9446-9451	NN	denotes	Hnf3β
T2654	9451-9452	HYPH	denotes	-
T2653	9452-9460	JJ	denotes	reactive
T2656	9461-9471	JJ	denotes	definitive
T2655	9472-9480	NN	denotes	endoderm
T2657	9481-9485	IN	denotes	over
T2658	9486-9489	DT	denotes	the
T2660	9490-9502	JJ	denotes	disorganized
T2661	9503-9511	JJ	denotes	anterior
T2662	9512-9518	NN	denotes	streak
T2659	9519-9525	NN	denotes	region
T2663	9526-9529	CC	denotes	and
T2664	9530-9540	VBG	denotes	proceeding
T2665	9541-9550	RB	denotes	rostrally
T2666	9551-9557	IN	denotes	around
T2667	9558-9561	DT	denotes	the
T2669	9562-9568	JJ	denotes	distal
T2668	9569-9572	NN	denotes	tip
T2670	9573-9574	-LRB-	denotes	(
T2672	9574-9578	NN	denotes	Fig.
T2673	9579-9581	CD	denotes	1H
T2674	9581-9582	,	denotes	,
T2671	9582-9583	NN	denotes	J
T2675	9583-9584	-RRB-	denotes	)
T2676	9584-9585	.	denotes	.
T2677	9585-9662	sentence	denotes	In both normal and mutant embryos, the AVE exhibits little Hnf3β expression.
T2678	9586-9588	IN	denotes	In
T2680	9589-9593	CC	denotes	both
T2681	9594-9600	JJ	denotes	normal
T2683	9601-9604	CC	denotes	and
T2684	9605-9611	NN	denotes	mutant
T2682	9612-9619	NNS	denotes	embryos
T2685	9619-9621	,	denotes	,
T2686	9621-9624	DT	denotes	the
T2687	9625-9628	NN	denotes	AVE
T2679	9629-9637	VBZ	denotes	exhibits
T2688	9638-9644	JJ	denotes	little
T2690	9645-9650	NN	denotes	Hnf3β
T2689	9651-9661	NN	denotes	expression
T2691	9661-9662	.	denotes	.
T2692	9662-9784	sentence	denotes	Therefore, huntingtin deficiency does not greatly affect Hnf3β regulation or the reorganization of the visceral endoderm.
T2693	9663-9672	RB	denotes	Therefore
T2695	9672-9674	,	denotes	,
T2696	9674-9684	NN	denotes	huntingtin
T2697	9685-9695	NN	denotes	deficiency
T2698	9696-9700	VBZ	denotes	does
T2699	9701-9704	RB	denotes	not
T2700	9705-9712	RB	denotes	greatly
T2694	9713-9719	VB	denotes	affect
T2701	9720-9725	NN	denotes	Hnf3β
T2702	9726-9736	NN	denotes	regulation
T2703	9737-9739	CC	denotes	or
T2704	9740-9743	DT	denotes	the
T2705	9744-9758	NN	denotes	reorganization
T2706	9759-9761	IN	denotes	of
T2707	9762-9765	DT	denotes	the
T2709	9766-9774	JJ	denotes	visceral
T2708	9775-9783	NN	denotes	endoderm
T2710	9783-9784	.	denotes	.
T2711	9784-10000	sentence	denotes	The lack of a morphological node and presence of a shortened streak, together with reduced neuroectoderm and lack of headfolds, suggest that anterior streak formation may be impaired in huntingtin deficient embryos.
T2712	9785-9788	DT	denotes	The
T2713	9789-9793	NN	denotes	lack
T2715	9794-9796	IN	denotes	of
T2716	9797-9798	DT	denotes	a
T2718	9799-9812	JJ	denotes	morphological
T2717	9813-9817	NN	denotes	node
T2719	9818-9821	CC	denotes	and
T2720	9822-9830	NN	denotes	presence
T2721	9831-9833	IN	denotes	of
T2722	9834-9835	DT	denotes	a
T2724	9836-9845	JJ	denotes	shortened
T2723	9846-9852	NN	denotes	streak
T2725	9852-9854	,	denotes	,
T2726	9854-9862	RB	denotes	together
T2727	9863-9867	IN	denotes	with
T2728	9868-9875	VBN	denotes	reduced
T2729	9876-9889	NN	denotes	neuroectoderm
T2730	9890-9893	CC	denotes	and
T2731	9894-9898	NN	denotes	lack
T2732	9899-9901	IN	denotes	of
T2733	9902-9911	NNS	denotes	headfolds
T2734	9911-9913	,	denotes	,
T2714	9913-9920	VBP	denotes	suggest
T2735	9921-9925	IN	denotes	that
T2737	9926-9934	JJ	denotes	anterior
T2738	9935-9941	NN	denotes	streak
T2739	9942-9951	NN	denotes	formation
T2740	9952-9955	MD	denotes	may
T2741	9956-9958	VB	denotes	be
T2736	9959-9967	VBN	denotes	impaired
T2742	9968-9970	IN	denotes	in
T2743	9971-9981	NN	denotes	huntingtin
T2744	9982-9991	JJ	denotes	deficient
T2745	9992-9999	NNS	denotes	embryos
T2746	9999-10000	.	denotes	.
T2747	10000-10083	sentence	denotes	To investigate this possibility, we examined mesoderm formation in mutant embryos.
T2748	10001-10003	TO	denotes	To
T2749	10004-10015	VB	denotes	investigate
T2751	10016-10020	DT	denotes	this
T2752	10021-10032	NN	denotes	possibility
T2753	10032-10034	,	denotes	,
T2754	10034-10036	PRP	denotes	we
T2750	10037-10045	VBD	denotes	examined
T2755	10046-10054	NN	denotes	mesoderm
T2756	10055-10064	NN	denotes	formation
T2757	10065-10067	IN	denotes	in
T2758	10068-10074	NN	denotes	mutant
T2759	10075-10082	NNS	denotes	embryos
T2760	10082-10083	.	denotes	.
T2761	10083-10195	sentence	denotes	Mesoderm is specified in the mutant embryos, as marked by the expression of T (Brachyury) and Evx1 (Fig. 2A–F).
T2762	10084-10092	NN	denotes	Mesoderm
T2764	10093-10095	VBZ	denotes	is
T2763	10096-10105	VBN	denotes	specified
T2765	10106-10108	IN	denotes	in
T2766	10109-10112	DT	denotes	the
T2768	10113-10119	NN	denotes	mutant
T2767	10120-10127	NNS	denotes	embryos
T2769	10127-10129	,	denotes	,
T2770	10129-10131	IN	denotes	as
T2771	10132-10138	VBN	denotes	marked
T2772	10139-10141	IN	denotes	by
T2773	10142-10145	DT	denotes	the
T2774	10146-10156	NN	denotes	expression
T2775	10157-10159	IN	denotes	of
T2776	10160-10161	NN	denotes	T
T2777	10162-10163	-LRB-	denotes	(
T2778	10163-10172	NN	denotes	Brachyury
T2779	10172-10173	-RRB-	denotes	)
T2780	10174-10177	CC	denotes	and
T2781	10178-10182	NN	denotes	Evx1
T2782	10183-10184	-LRB-	denotes	(
T2783	10184-10188	NN	denotes	Fig.
T2784	10189-10191	CD	denotes	2A
T2785	10191-10192	SYM	denotes	–
T2786	10192-10193	NN	denotes	F
T2787	10193-10194	-RRB-	denotes	)
T2788	10194-10195	.	denotes	.
T2789	10195-10301	sentence	denotes	However, close inspection of the data reveals abnormal patterning within this tissue and its derivatives.
T2790	10196-10203	RB	denotes	However
T2792	10203-10205	,	denotes	,
T2793	10205-10210	JJ	denotes	close
T2794	10211-10221	NN	denotes	inspection
T2795	10222-10224	IN	denotes	of
T2796	10225-10228	DT	denotes	the
T2797	10229-10233	NNS	denotes	data
T2791	10234-10241	VBZ	denotes	reveals
T2798	10242-10250	JJ	denotes	abnormal
T2799	10251-10261	NN	denotes	patterning
T2800	10262-10268	IN	denotes	within
T2801	10269-10273	DT	denotes	this
T2802	10274-10280	NN	denotes	tissue
T2803	10281-10284	CC	denotes	and
T2804	10285-10288	PRP$	denotes	its
T2805	10289-10300	NNS	denotes	derivatives
T2806	10300-10301	.	denotes	.
T2807	10301-10302	NN	denotes	T
T2809	10301-10557	sentence	denotes	T, normally expressed in the primitive streak, node and axial head process/notochord mesoderm [22], is detected in the shortened streak and axial mesoderm in Hdhex4/5/Hdhex4/5 embryos, extending rostrally from a region of weakly positive cells (Fig. 2A,B).
T2810	10302-10304	,	denotes	,
T2811	10304-10312	RB	denotes	normally
T2812	10313-10322	VBN	denotes	expressed
T2813	10323-10325	IN	denotes	in
T2814	10326-10329	DT	denotes	the
T2816	10330-10339	JJ	denotes	primitive
T2815	10340-10346	NN	denotes	streak
T2817	10346-10348	,	denotes	,
T2818	10348-10352	NN	denotes	node
T2819	10353-10356	CC	denotes	and
T2820	10357-10362	JJ	denotes	axial
T2822	10363-10367	NN	denotes	head
T2821	10368-10375	NN	denotes	process
T2823	10375-10376	HYPH	denotes	/
T2824	10376-10385	NN	denotes	notochord
T2825	10386-10394	NN	denotes	mesoderm
T2826	10395-10396	-LRB-	denotes	[
T2827	10396-10398	CD	denotes	22
T2828	10398-10399	-RRB-	denotes	]
T2829	10399-10401	,	denotes	,
T2830	10401-10403	VBZ	denotes	is
T2808	10404-10412	VBN	denotes	detected
T2831	10413-10415	IN	denotes	in
T2832	10416-10419	DT	denotes	the
T2834	10420-10429	VBN	denotes	shortened
T2833	10430-10436	NN	denotes	streak
T2835	10437-10440	CC	denotes	and
T2836	10441-10446	JJ	denotes	axial
T2837	10447-10455	NN	denotes	mesoderm
T2838	10456-10458	IN	denotes	in
T2839	10459-10465	NN	denotes	Hdhex4
T2841	10465-10466	HYPH	denotes	/
T2842	10466-10467	CD	denotes	5
T2843	10467-10468	HYPH	denotes	/
T2844	10468-10474	NN	denotes	Hdhex4
T2845	10474-10475	HYPH	denotes	/
T2846	10475-10476	CD	denotes	5
T2840	10477-10484	NNS	denotes	embryos
T2847	10484-10486	,	denotes	,
T2848	10486-10495	VBG	denotes	extending
T2849	10496-10505	RB	denotes	rostrally
T2850	10506-10510	IN	denotes	from
T2851	10511-10512	DT	denotes	a
T2852	10513-10519	NN	denotes	region
T2853	10520-10522	IN	denotes	of
T2854	10523-10529	RB	denotes	weakly
T2855	10530-10538	JJ	denotes	positive
T2856	10539-10544	NNS	denotes	cells
T2857	10545-10546	-LRB-	denotes	(
T2859	10546-10550	NNP	denotes	Fig.
T2860	10551-10553	CD	denotes	2A
T2861	10553-10554	,	denotes	,
T2858	10554-10555	NN	denotes	B
T2862	10555-10556	-RRB-	denotes	)
T2863	10556-10557	.	denotes	.
T2864	10557-10691	sentence	denotes	T expression appears weaker, however, in the anterior streak, corresponding to cells that will give rise to axial mesoderm (Fig. 2D).
T2865	10558-10559	NN	denotes	T
T2866	10560-10570	NN	denotes	expression
T2867	10571-10578	VBZ	denotes	appears
T2868	10579-10585	JJR	denotes	weaker
T2869	10585-10587	,	denotes	,
T2870	10587-10594	RB	denotes	however
T2871	10594-10596	,	denotes	,
T2872	10596-10598	IN	denotes	in
T2873	10599-10602	DT	denotes	the
T2875	10603-10611	JJ	denotes	anterior
T2874	10612-10618	NN	denotes	streak
T2876	10618-10620	,	denotes	,
T2877	10620-10633	VBG	denotes	corresponding
T2878	10634-10636	IN	denotes	to
T2879	10637-10642	NNS	denotes	cells
T2880	10643-10647	WDT	denotes	that
T2882	10648-10652	MD	denotes	will
T2881	10653-10657	VB	denotes	give
T2883	10658-10662	NN	denotes	rise
T2884	10663-10665	IN	denotes	to
T2885	10666-10671	JJ	denotes	axial
T2886	10672-10680	NN	denotes	mesoderm
T2887	10681-10682	-LRB-	denotes	(
T2888	10682-10686	NN	denotes	Fig.
T2889	10687-10689	CD	denotes	2D
T2890	10689-10690	-RRB-	denotes	)
T2891	10690-10691	.	denotes	.
T2892	10691-10827	sentence	denotes	T is also ectopically expressed in mutant extraembryonic mesoderm at the anterior embryonic junction and along the chorion (Fig. 2B,D).
T2893	10692-10693	NN	denotes	T
T2895	10694-10696	VBZ	denotes	is
T2896	10697-10701	RB	denotes	also
T2897	10702-10713	RB	denotes	ectopically
T2894	10714-10723	VBN	denotes	expressed
T2898	10724-10726	IN	denotes	in
T2899	10727-10733	NN	denotes	mutant
T2901	10734-10748	JJ	denotes	extraembryonic
T2900	10749-10757	NN	denotes	mesoderm
T2902	10758-10760	IN	denotes	at
T2903	10761-10764	DT	denotes	the
T2905	10765-10773	JJ	denotes	anterior
T2906	10774-10783	JJ	denotes	embryonic
T2904	10784-10792	NN	denotes	junction
T2907	10793-10796	CC	denotes	and
T2908	10797-10802	IN	denotes	along
T2909	10803-10806	DT	denotes	the
T2910	10807-10814	NN	denotes	chorion
T2911	10815-10816	-LRB-	denotes	(
T2913	10816-10820	NN	denotes	Fig.
T2914	10821-10823	CD	denotes	2B
T2915	10823-10824	,	denotes	,
T2912	10824-10825	NN	denotes	D
T2916	10825-10826	-RRB-	denotes	)
T2917	10826-10827	.	denotes	.
T2918	10827-11098	sentence	denotes	Similarly, Evx1, normally expressed in primitive streak mesoderm at E7.5 with highest levels in proximal cells [23], is expressed in the proximal shortened streak but is also aberrantly expressed throughout the extraembryonic mesoderm, allantois and chorion (Fig. 2E,F).
T2919	10828-10837	RB	denotes	Similarly
T2921	10837-10839	,	denotes	,
T2922	10839-10843	NN	denotes	Evx1
T2923	10843-10845	,	denotes	,
T2924	10845-10853	RB	denotes	normally
T2925	10854-10863	VBN	denotes	expressed
T2926	10864-10866	IN	denotes	in
T2927	10867-10876	JJ	denotes	primitive
T2929	10877-10883	NN	denotes	streak
T2928	10884-10892	NN	denotes	mesoderm
T2930	10893-10895	IN	denotes	at
T2931	10896-10900	NN	denotes	E7.5
T2932	10901-10905	IN	denotes	with
T2934	10906-10913	JJS	denotes	highest
T2933	10914-10920	NNS	denotes	levels
T2935	10921-10923	IN	denotes	in
T2936	10924-10932	JJ	denotes	proximal
T2937	10933-10938	NNS	denotes	cells
T2938	10939-10940	-LRB-	denotes	[
T2939	10940-10942	CD	denotes	23
T2940	10942-10943	-RRB-	denotes	]
T2941	10943-10945	,	denotes	,
T2942	10945-10947	VBZ	denotes	is
T2920	10948-10957	VBN	denotes	expressed
T2943	10958-10960	IN	denotes	in
T2944	10961-10964	DT	denotes	the
T2946	10965-10973	JJ	denotes	proximal
T2947	10974-10983	VBN	denotes	shortened
T2945	10984-10990	NN	denotes	streak
T2948	10991-10994	CC	denotes	but
T2949	10995-10997	VBZ	denotes	is
T2951	10998-11002	RB	denotes	also
T2952	11003-11013	RB	denotes	aberrantly
T2950	11014-11023	VBN	denotes	expressed
T2953	11024-11034	IN	denotes	throughout
T2954	11035-11038	DT	denotes	the
T2956	11039-11053	JJ	denotes	extraembryonic
T2955	11054-11062	NN	denotes	mesoderm
T2957	11062-11064	,	denotes	,
T2958	11064-11073	NN	denotes	allantois
T2959	11074-11077	CC	denotes	and
T2960	11078-11085	NN	denotes	chorion
T2961	11086-11087	-LRB-	denotes	(
T2963	11087-11091	NN	denotes	Fig.
T2964	11092-11094	CD	denotes	2E
T2965	11094-11095	,	denotes	,
T2962	11095-11096	NN	denotes	F
T2966	11096-11097	-RRB-	denotes	)
T2967	11097-11098	.	denotes	.
T2968	11098-11220	sentence	denotes	Extraembryonic mesoderm, derived from the proximal streak, does not normally express T or Evx1 in wild-type embryos [22].
T2969	11099-11113	JJ	denotes	Extraembryonic
T2970	11114-11122	NN	denotes	mesoderm
T2972	11122-11124	,	denotes	,
T2973	11124-11131	VBN	denotes	derived
T2974	11132-11136	IN	denotes	from
T2975	11137-11140	DT	denotes	the
T2977	11141-11149	JJ	denotes	proximal
T2976	11150-11156	NN	denotes	streak
T2978	11156-11158	,	denotes	,
T2979	11158-11162	VBZ	denotes	does
T2980	11163-11166	RB	denotes	not
T2981	11167-11175	RB	denotes	normally
T2971	11176-11183	VB	denotes	express
T2982	11184-11185	NN	denotes	T
T2983	11186-11188	CC	denotes	or
T2984	11189-11193	NN	denotes	Evx1
T2985	11194-11196	IN	denotes	in
T2986	11197-11201	JJ	denotes	wild
T2988	11201-11202	HYPH	denotes	-
T2987	11202-11206	NN	denotes	type
T2989	11207-11214	NNS	denotes	embryos
T2990	11215-11216	-LRB-	denotes	[
T2991	11216-11218	CD	denotes	22
T2992	11218-11219	-RRB-	denotes	]
T2993	11219-11220	.	denotes	.
T2994	11220-11431	sentence	denotes	Therefore, the inappropriate expression of T and Evx1, the shortened primitive streak, and the absence of a morphological node, all suggest that the anterior primitive streak is deficient in the mutant embryos.
T2995	11221-11230	RB	denotes	Therefore
T2997	11230-11232	,	denotes	,
T2998	11232-11235	DT	denotes	the
T3000	11236-11249	JJ	denotes	inappropriate
T2999	11250-11260	NN	denotes	expression
T3001	11261-11263	IN	denotes	of
T3002	11264-11265	NN	denotes	T
T3003	11266-11269	CC	denotes	and
T3004	11270-11274	NN	denotes	Evx1
T3005	11274-11276	,	denotes	,
T3006	11276-11279	DT	denotes	the
T3008	11280-11289	VBN	denotes	shortened
T3009	11290-11299	JJ	denotes	primitive
T3007	11300-11306	NN	denotes	streak
T3010	11306-11308	,	denotes	,
T3011	11308-11311	CC	denotes	and
T3012	11312-11315	DT	denotes	the
T3013	11316-11323	NN	denotes	absence
T3014	11324-11326	IN	denotes	of
T3015	11327-11328	DT	denotes	a
T3017	11329-11342	JJ	denotes	morphological
T3016	11343-11347	NN	denotes	node
T3018	11347-11349	,	denotes	,
T3019	11349-11352	DT	denotes	all
T2996	11353-11360	VBP	denotes	suggest
T3020	11361-11365	IN	denotes	that
T3022	11366-11369	DT	denotes	the
T3024	11370-11378	JJ	denotes	anterior
T3025	11379-11388	JJ	denotes	primitive
T3023	11389-11395	NN	denotes	streak
T3021	11396-11398	VBZ	denotes	is
T3026	11399-11408	JJ	denotes	deficient
T3027	11409-11411	IN	denotes	in
T3028	11412-11415	DT	denotes	the
T3030	11416-11422	NN	denotes	mutant
T3029	11423-11430	NNS	denotes	embryos
T3031	11430-11431	.	denotes	.
T3032	11431-12780	sentence	denotes	Figure 2 Huntingtin is required for formation of anterior primitive streak and paraxial mesoderm. Whole mount and section insitu hybridizations of E7.5 embryos shows T (Brachyury) (A-D) is expressed in the primitive streak, node, axial mesoderm and Evx1 (E-F) is expressed in the primitive streak, most strongly in the proximal streak wild-type embryos. However, in mutant embryos, both T (B, D) and Evx1 (F) are ectopically expressed in the extraembryonic region. Wnt3A expression is reduced in mutant embryos (H), although the localization of its expression to the proximal streak is the same as in wild-type embryos (G). Analysis of paraxial mesoderm markers Tbx6 (I,J) and Dll1 (K,L), reveals that these markers are reduced in mutant embryos (J,L), suggesting impaired paraxial mesoderm production in the absence of huntingtin. Embryos in A-H are shown in a lateral view with anterior oriented to the left. Embryos in I-L are shown in a posterior view (I,K) or near posterior (J,L) view with proximal oriented toward the top. In (C,D), al = allantois, a = amnion, ch = chorion, ee = embryonic node(N), em = extraembryonic mesoderm, n = node, ps = primitive streak. Rather than a node, mutant embryos exhibit a region of disorganized cells (*) at the distal extent of the short primitive streak. The anterior streak generates paraxial mesoderm.
T8760	11442-11452	NN	denotes	Huntingtin
T8762	11453-11455	VBZ	denotes	is
T8761	11456-11464	VBN	denotes	required
T8763	11465-11468	IN	denotes	for
T8764	11469-11478	NN	denotes	formation
T8765	11479-11481	IN	denotes	of
T8766	11482-11490	JJ	denotes	anterior
T8768	11491-11500	JJ	denotes	primitive
T8767	11501-11507	NN	denotes	streak
T8769	11508-11511	CC	denotes	and
T8770	11512-11520	JJ	denotes	paraxial
T8771	11521-11529	NN	denotes	mesoderm
T8772	11529-11530	.	denotes	.
T8773	11530-11786	sentence	denotes	Whole mount and section insitu hybridizations of E7.5 embryos shows T (Brachyury) (A-D) is expressed in the primitive streak, node, axial mesoderm and Evx1 (E-F) is expressed in the primitive streak, most strongly in the proximal streak wild-type embryos.
T8774	11531-11536	JJ	denotes	Whole
T8775	11537-11542	NN	denotes	mount
T8777	11543-11546	CC	denotes	and
T8778	11547-11554	NN	denotes	section
T8779	11555-11561	FW	denotes	insitu
T8776	11562-11576	NNS	denotes	hybridizations
T8781	11577-11579	IN	denotes	of
T8782	11580-11584	NN	denotes	E7.5
T8783	11585-11592	NNS	denotes	embryos
T8780	11593-11598	VBZ	denotes	shows
T8784	11599-11600	NN	denotes	T
T8786	11601-11602	-LRB-	denotes	(
T8787	11602-11611	NNP	denotes	Brachyury
T8788	11611-11612	-RRB-	denotes	)
T8789	11613-11614	-LRB-	denotes	(
T8790	11614-11615	NN	denotes	A
T8791	11615-11616	SYM	denotes	-
T8792	11616-11617	NN	denotes	D
T8793	11617-11618	-RRB-	denotes	)
T8794	11619-11621	VBZ	denotes	is
T8785	11622-11631	VBN	denotes	expressed
T8795	11632-11634	IN	denotes	in
T8796	11635-11638	DT	denotes	the
T8798	11639-11648	JJ	denotes	primitive
T8797	11649-11655	NN	denotes	streak
T8799	11655-11657	,	denotes	,
T8800	11657-11661	NN	denotes	node
T8801	11661-11663	,	denotes	,
T8802	11663-11668	JJ	denotes	axial
T8803	11669-11677	NN	denotes	mesoderm
T8804	11678-11681	CC	denotes	and
T8805	11682-11686	NN	denotes	Evx1
T8807	11687-11688	-LRB-	denotes	(
T8808	11688-11689	NN	denotes	E
T8809	11689-11690	SYM	denotes	-
T8810	11690-11691	NN	denotes	F
T8811	11691-11692	-RRB-	denotes	)
T8812	11693-11695	VBZ	denotes	is
T8806	11696-11705	VBN	denotes	expressed
T8813	11706-11708	IN	denotes	in
T8814	11709-11712	DT	denotes	the
T8816	11713-11722	JJ	denotes	primitive
T8815	11723-11729	NN	denotes	streak
T8817	11729-11731	,	denotes	,
T8818	11731-11735	RBS	denotes	most
T8819	11736-11744	RB	denotes	strongly
T8820	11745-11747	IN	denotes	in
T8821	11748-11751	DT	denotes	the
T8823	11752-11760	JJ	denotes	proximal
T8824	11761-11767	NN	denotes	streak
T8825	11768-11772	JJ	denotes	wild
T8827	11772-11773	HYPH	denotes	-
T8826	11773-11777	NN	denotes	type
T8822	11778-11785	NNS	denotes	embryos
T8828	11785-11786	.	denotes	.
T8829	11786-11897	sentence	denotes	However, in mutant embryos, both T (B, D) and Evx1 (F) are ectopically expressed in the extraembryonic region.
T8830	11787-11794	RB	denotes	However
T8832	11794-11796	,	denotes	,
T8833	11796-11798	IN	denotes	in
T8834	11799-11805	NN	denotes	mutant
T8835	11806-11813	NNS	denotes	embryos
T8836	11813-11815	,	denotes	,
T8837	11815-11819	CC	denotes	both
T8838	11820-11821	NN	denotes	T
T8839	11822-11823	-LRB-	denotes	(
T8841	11823-11824	NN	denotes	B
T8842	11824-11826	,	denotes	,
T8840	11826-11827	NN	denotes	D
T8843	11827-11828	-RRB-	denotes	)
T8844	11829-11832	CC	denotes	and
T8845	11833-11837	NN	denotes	Evx1
T8846	11838-11839	-LRB-	denotes	(
T8847	11839-11840	NN	denotes	F
T8848	11840-11841	-RRB-	denotes	)
T8849	11842-11845	VBP	denotes	are
T8850	11846-11857	RB	denotes	ectopically
T8831	11858-11867	VBN	denotes	expressed
T8851	11868-11870	IN	denotes	in
T8852	11871-11874	DT	denotes	the
T8854	11875-11889	JJ	denotes	extraembryonic
T8853	11890-11896	NN	denotes	region
T8855	11896-11897	.	denotes	.
T8856	11897-12056	sentence	denotes	Wnt3A expression is reduced in mutant embryos (H), although the localization of its expression to the proximal streak is the same as in wild-type embryos (G).
T8857	11898-11903	NN	denotes	Wnt3A
T8858	11904-11914	NN	denotes	expression
T8860	11915-11917	VBZ	denotes	is
T8859	11918-11925	VBN	denotes	reduced
T8861	11926-11928	IN	denotes	in
T8862	11929-11935	NN	denotes	mutant
T8863	11936-11943	NNS	denotes	embryos
T8864	11944-11945	-LRB-	denotes	(
T8865	11945-11946	NN	denotes	H
T8866	11946-11947	-RRB-	denotes	)
T8867	11947-11949	,	denotes	,
T8868	11949-11957	IN	denotes	although
T8870	11958-11961	DT	denotes	the
T8871	11962-11974	NN	denotes	localization
T8872	11975-11977	IN	denotes	of
T8873	11978-11981	PRP$	denotes	its
T8874	11982-11992	NN	denotes	expression
T8875	11993-11995	IN	denotes	to
T8876	11996-11999	DT	denotes	the
T8878	12000-12008	JJ	denotes	proximal
T8877	12009-12015	NN	denotes	streak
T8869	12016-12018	VBZ	denotes	is
T8879	12019-12022	DT	denotes	the
T8880	12023-12027	JJ	denotes	same
T8881	12028-12030	IN	denotes	as
T8882	12031-12033	IN	denotes	in
T8883	12034-12038	JJ	denotes	wild
T8885	12038-12039	HYPH	denotes	-
T8884	12039-12043	NN	denotes	type
T8886	12044-12051	NNS	denotes	embryos
T8887	12052-12053	-LRB-	denotes	(
T8888	12053-12054	NN	denotes	G
T8889	12054-12055	-RRB-	denotes	)
T8890	12055-12056	.	denotes	.
T8891	12056-12264	sentence	denotes	Analysis of paraxial mesoderm markers Tbx6 (I,J) and Dll1 (K,L), reveals that these markers are reduced in mutant embryos (J,L), suggesting impaired paraxial mesoderm production in the absence of huntingtin.
T8892	12057-12065	NN	denotes	Analysis
T8894	12066-12068	IN	denotes	of
T8895	12069-12077	JJ	denotes	paraxial
T8897	12078-12086	NN	denotes	mesoderm
T8898	12087-12094	NNS	denotes	markers
T8896	12095-12099	NN	denotes	Tbx6
T8899	12100-12101	-LRB-	denotes	(
T8901	12101-12102	NN	denotes	I
T8902	12102-12103	,	denotes	,
T8900	12103-12104	NN	denotes	J
T8903	12104-12105	-RRB-	denotes	)
T8904	12106-12109	CC	denotes	and
T8905	12110-12114	NN	denotes	Dll1
T8906	12115-12116	-LRB-	denotes	(
T8908	12116-12117	NN	denotes	K
T8909	12117-12118	,	denotes	,
T8907	12118-12119	NN	denotes	L
T8910	12119-12120	-RRB-	denotes	)
T8911	12120-12122	,	denotes	,
T8893	12122-12129	VBZ	denotes	reveals
T8912	12130-12134	IN	denotes	that
T8914	12135-12140	DT	denotes	these
T8915	12141-12148	NNS	denotes	markers
T8916	12149-12152	VBP	denotes	are
T8913	12153-12160	VBN	denotes	reduced
T8917	12161-12163	IN	denotes	in
T8918	12164-12170	NN	denotes	mutant
T8919	12171-12178	NNS	denotes	embryos
T8920	12179-12180	-LRB-	denotes	(
T8922	12180-12181	NN	denotes	J
T8923	12181-12182	,	denotes	,
T8921	12182-12183	NN	denotes	L
T8924	12183-12184	-RRB-	denotes	)
T8925	12184-12186	,	denotes	,
T8926	12186-12196	VBG	denotes	suggesting
T8927	12197-12205	VBN	denotes	impaired
T8929	12206-12214	JJ	denotes	paraxial
T8930	12215-12223	NN	denotes	mesoderm
T8928	12224-12234	NN	denotes	production
T8931	12235-12237	IN	denotes	in
T8932	12238-12241	DT	denotes	the
T8933	12242-12249	NN	denotes	absence
T8934	12250-12252	IN	denotes	of
T8935	12253-12263	NN	denotes	huntingtin
T8936	12263-12264	.	denotes	.
T8937	12264-12343	sentence	denotes	Embryos in A-H are shown in a lateral view with anterior oriented to the left.
T8938	12265-12272	NNS	denotes	Embryos
T8940	12273-12275	IN	denotes	in
T8941	12276-12277	NN	denotes	A
T8942	12277-12278	SYM	denotes	-
T8943	12278-12279	NN	denotes	H
T8944	12280-12283	VBP	denotes	are
T8939	12284-12289	VBN	denotes	shown
T8945	12290-12292	IN	denotes	in
T8946	12293-12294	DT	denotes	a
T8948	12295-12302	JJ	denotes	lateral
T8947	12303-12307	NN	denotes	view
T8949	12308-12312	IN	denotes	with
T8951	12313-12321	NN	denotes	anterior
T8950	12322-12330	VBN	denotes	oriented
T8952	12331-12333	IN	denotes	to
T8953	12334-12337	DT	denotes	the
T8954	12338-12342	NN	denotes	left
T8955	12342-12343	.	denotes	.
T8956	12343-12462	sentence	denotes	Embryos in I-L are shown in a posterior view (I,K) or near posterior (J,L) view with proximal oriented toward the top.
T8957	12344-12351	NNS	denotes	Embryos
T8959	12352-12354	IN	denotes	in
T8960	12355-12356	NN	denotes	I
T8961	12356-12357	SYM	denotes	-
T8962	12357-12358	NN	denotes	L
T8963	12359-12362	VBP	denotes	are
T8958	12363-12368	VBN	denotes	shown
T8964	12369-12371	IN	denotes	in
T8965	12372-12373	DT	denotes	a
T8967	12374-12383	JJ	denotes	posterior
T8966	12384-12388	NN	denotes	view
T8968	12389-12390	-LRB-	denotes	(
T8970	12390-12391	NN	denotes	I
T8971	12391-12392	,	denotes	,
T8969	12392-12393	NN	denotes	K
T8972	12393-12394	-RRB-	denotes	)
T8973	12395-12397	CC	denotes	or
T8974	12398-12402	RB	denotes	near
T8975	12403-12412	JJ	denotes	posterior
T8977	12413-12414	-LRB-	denotes	(
T8979	12414-12415	NN	denotes	J
T8980	12415-12416	,	denotes	,
T8978	12416-12417	NN	denotes	L
T8981	12417-12418	-RRB-	denotes	)
T8976	12419-12423	NN	denotes	view
T8982	12424-12428	IN	denotes	with
T8984	12429-12437	JJ	denotes	proximal
T8983	12438-12446	VBN	denotes	oriented
T8985	12447-12453	IN	denotes	toward
T8986	12454-12457	DT	denotes	the
T8987	12458-12461	NN	denotes	top
T8988	12461-12462	.	denotes	.
T8989	12462-12601	sentence	denotes	In (C,D), al = allantois, a = amnion, ch = chorion, ee = embryonic node(N), em = extraembryonic mesoderm, n = node, ps = primitive streak.
T8990	12463-12465	IN	denotes	In
T8992	12466-12467	-LRB-	denotes	(
T8994	12467-12468	NN	denotes	C
T8995	12468-12469	,	denotes	,
T8993	12469-12470	NN	denotes	D
T8996	12470-12471	-RRB-	denotes	)
T8997	12471-12473	,	denotes	,
T8998	12473-12475	NN	denotes	al
T9000	12476-12477	SYM	denotes	=
T8999	12478-12487	NN	denotes	allantois
T9001	12487-12489	,	denotes	,
T9002	12489-12490	NN	denotes	a
T9004	12491-12492	SYM	denotes	=
T9003	12493-12499	NN	denotes	amnion
T9005	12499-12501	,	denotes	,
T9006	12501-12503	NN	denotes	ch
T9008	12504-12505	SYM	denotes	=
T9007	12506-12513	NN	denotes	chorion
T9009	12513-12515	,	denotes	,
T9010	12515-12517	NN	denotes	ee
T9012	12518-12519	SYM	denotes	=
T9013	12520-12529	JJ	denotes	embryonic
T9011	12530-12534	NN	denotes	node
T9014	12534-12535	-LRB-	denotes	(
T9015	12535-12536	NN	denotes	N
T9016	12536-12537	-RRB-	denotes	)
T9017	12537-12539	,	denotes	,
T9018	12539-12541	NN	denotes	em
T9020	12542-12543	SYM	denotes	=
T9021	12544-12558	JJ	denotes	extraembryonic
T9019	12559-12567	NN	denotes	mesoderm
T9022	12567-12569	,	denotes	,
T9023	12569-12570	NN	denotes	n
T9025	12571-12572	SYM	denotes	=
T9024	12573-12577	NN	denotes	node
T9026	12577-12579	,	denotes	,
T9027	12579-12581	NN	denotes	ps
T9028	12582-12583	SYM	denotes	=
T9029	12584-12593	JJ	denotes	primitive
T8991	12594-12600	NN	denotes	streak
T9030	12600-12601	.	denotes	.
T9031	12601-12731	sentence	denotes	Rather than a node, mutant embryos exhibit a region of disorganized cells (*) at the distal extent of the short primitive streak.
T9032	12602-12608	RB	denotes	Rather
T9033	12609-12613	IN	denotes	than
T9035	12614-12615	DT	denotes	a
T9036	12616-12620	NN	denotes	node
T9037	12620-12622	,	denotes	,
T9038	12622-12628	NN	denotes	mutant
T9039	12629-12636	NNS	denotes	embryos
T9034	12637-12644	VBP	denotes	exhibit
T9040	12645-12646	DT	denotes	a
T9041	12647-12653	NN	denotes	region
T9042	12654-12656	IN	denotes	of
T9043	12657-12669	VBN	denotes	disorganized
T9044	12670-12675	NNS	denotes	cells
T9045	12676-12677	-LRB-	denotes	(
T9046	12677-12678	SYM	denotes	*
T9047	12678-12679	-RRB-	denotes	)
T9048	12680-12682	IN	denotes	at
T9049	12683-12686	DT	denotes	the
T9051	12687-12693	JJ	denotes	distal
T9050	12694-12700	NN	denotes	extent
T9052	12701-12703	IN	denotes	of
T9053	12704-12707	DT	denotes	the
T9055	12708-12713	JJ	denotes	short
T9056	12714-12723	JJ	denotes	primitive
T9054	12724-12730	NN	denotes	streak
T9057	12730-12731	.	denotes	.
T3033	12732-12735	DT	denotes	The
T3035	12736-12744	JJ	denotes	anterior
T3034	12745-12751	NN	denotes	streak
T3036	12752-12761	VBZ	denotes	generates
T3037	12762-12770	JJ	denotes	paraxial
T3038	12771-12779	NN	denotes	mesoderm
T3039	12779-12780	.	denotes	.
T3040	12780-12906	sentence	denotes	Therefore we examined paraxial mesoderm formation in wild-type and mutant embryos, revealing deficits in mesoderm patterning.
T3041	12781-12790	RB	denotes	Therefore
T3043	12791-12793	PRP	denotes	we
T3042	12794-12802	VBD	denotes	examined
T3044	12803-12811	JJ	denotes	paraxial
T3045	12812-12820	NN	denotes	mesoderm
T3046	12821-12830	NN	denotes	formation
T3047	12831-12833	IN	denotes	in
T3048	12834-12838	JJ	denotes	wild
T3050	12838-12839	HYPH	denotes	-
T3049	12839-12843	NN	denotes	type
T3052	12844-12847	CC	denotes	and
T3053	12848-12854	NN	denotes	mutant
T3051	12855-12862	NNS	denotes	embryos
T3054	12862-12864	,	denotes	,
T3055	12864-12873	VBG	denotes	revealing
T3056	12874-12882	NNS	denotes	deficits
T3057	12883-12885	IN	denotes	in
T3058	12886-12894	NN	denotes	mesoderm
T3059	12895-12905	NN	denotes	patterning
T3060	12905-12906	.	denotes	.
T3061	12906-13012	sentence	denotes	Starting at E.7.5, Wnt3A is expressed in the primitive streak in cells fated to become paraxial mesoderm.
T3062	12907-12915	VBG	denotes	Starting
T3064	12916-12918	IN	denotes	at
T3065	12919-12924	NN	denotes	E.7.5
T3066	12924-12926	,	denotes	,
T3067	12926-12931	NN	denotes	Wnt3A
T3068	12932-12934	VBZ	denotes	is
T3063	12935-12944	VBN	denotes	expressed
T3069	12945-12947	IN	denotes	in
T3070	12948-12951	DT	denotes	the
T3072	12952-12961	JJ	denotes	primitive
T3071	12962-12968	NN	denotes	streak
T3073	12969-12971	IN	denotes	in
T3074	12972-12977	NNS	denotes	cells
T3075	12978-12983	VBN	denotes	fated
T3076	12984-12986	TO	denotes	to
T3077	12987-12993	VB	denotes	become
T3078	12994-13002	JJ	denotes	paraxial
T3079	13003-13011	NN	denotes	mesoderm
T3080	13011-13012	.	denotes	.
T3081	13012-13201	sentence	denotes	In huntingtin deficient mutants, Wnt3a is induced in the proximal streak (Fig. 2G,H), confirming stage appropriate posterior development, in contrast to the absence of anterior head folds.
T3082	13013-13015	IN	denotes	In
T3084	13016-13026	NN	denotes	huntingtin
T3085	13027-13036	JJ	denotes	deficient
T3086	13037-13044	NNS	denotes	mutants
T3087	13044-13046	,	denotes	,
T3088	13046-13051	NN	denotes	Wnt3a
T3089	13052-13054	VBZ	denotes	is
T3083	13055-13062	VBN	denotes	induced
T3090	13063-13065	IN	denotes	in
T3091	13066-13069	DT	denotes	the
T3093	13070-13078	JJ	denotes	proximal
T3092	13079-13085	NN	denotes	streak
T3094	13086-13087	-LRB-	denotes	(
T3096	13087-13091	NN	denotes	Fig.
T3097	13092-13094	CD	denotes	2G
T3098	13094-13095	,	denotes	,
T3095	13095-13096	NN	denotes	H
T3099	13096-13097	-RRB-	denotes	)
T3100	13097-13099	,	denotes	,
T3101	13099-13109	VBG	denotes	confirming
T3102	13110-13115	NN	denotes	stage
T3103	13116-13127	JJ	denotes	appropriate
T3105	13128-13137	JJ	denotes	posterior
T3104	13138-13149	NN	denotes	development
T3106	13149-13151	,	denotes	,
T3107	13151-13153	IN	denotes	in
T3108	13154-13162	NN	denotes	contrast
T3109	13163-13165	IN	denotes	to
T3110	13166-13169	DT	denotes	the
T3111	13170-13177	NN	denotes	absence
T3112	13178-13180	IN	denotes	of
T3113	13181-13189	JJ	denotes	anterior
T3115	13190-13194	NN	denotes	head
T3114	13195-13200	NNS	denotes	folds
T3116	13200-13201	.	denotes	.
T3117	13201-13346	sentence	denotes	However, expression of Wnt3a is noticeably reduced in Hdhex4/5/Hdhex4/5 embryos, suggesting a defect in paraxial mesoderm development (Fig. 2H).
T3118	13202-13209	RB	denotes	However
T3120	13209-13211	,	denotes	,
T3121	13211-13221	NN	denotes	expression
T3122	13222-13224	IN	denotes	of
T3123	13225-13230	NN	denotes	Wnt3a
T3124	13231-13233	VBZ	denotes	is
T3125	13234-13244	RB	denotes	noticeably
T3119	13245-13252	VBN	denotes	reduced
T3126	13253-13255	IN	denotes	in
T3127	13256-13262	NN	denotes	Hdhex4
T3129	13262-13263	HYPH	denotes	/
T3130	13263-13264	CD	denotes	5
T3131	13264-13265	HYPH	denotes	/
T3132	13265-13271	NN	denotes	Hdhex4
T3133	13271-13272	HYPH	denotes	/
T3134	13272-13273	CD	denotes	5
T3128	13274-13281	NNS	denotes	embryos
T3135	13281-13283	,	denotes	,
T3136	13283-13293	VBG	denotes	suggesting
T3137	13294-13295	DT	denotes	a
T3138	13296-13302	NN	denotes	defect
T3139	13303-13305	IN	denotes	in
T3140	13306-13314	JJ	denotes	paraxial
T3141	13315-13323	NN	denotes	mesoderm
T3142	13324-13335	NN	denotes	development
T3143	13336-13337	-LRB-	denotes	(
T3144	13337-13341	NN	denotes	Fig.
T3145	13342-13344	CD	denotes	2H
T3146	13344-13345	-RRB-	denotes	)
T3147	13345-13346	.	denotes	.
T3148	13346-13481	sentence	denotes	Reduced expression of Tbx6 in the mesoderm lateral to the primitive streak in mutant embryos confirms this interpretation (Fig. 2I,J).
T3149	13347-13354	VBN	denotes	Reduced
T3150	13355-13365	NN	denotes	expression
T3152	13366-13368	IN	denotes	of
T3153	13369-13373	NN	denotes	Tbx6
T3154	13374-13376	IN	denotes	in
T3155	13377-13380	DT	denotes	the
T3156	13381-13389	NN	denotes	mesoderm
T3157	13390-13397	JJ	denotes	lateral
T3158	13398-13400	IN	denotes	to
T3159	13401-13404	DT	denotes	the
T3161	13405-13414	JJ	denotes	primitive
T3160	13415-13421	NN	denotes	streak
T3162	13422-13424	IN	denotes	in
T3163	13425-13431	NN	denotes	mutant
T3164	13432-13439	NNS	denotes	embryos
T3151	13440-13448	VBZ	denotes	confirms
T3165	13449-13453	DT	denotes	this
T3166	13454-13468	NN	denotes	interpretation
T3167	13469-13470	-LRB-	denotes	(
T3169	13470-13474	NN	denotes	Fig.
T3170	13475-13477	CD	denotes	2I
T3171	13477-13478	,	denotes	,
T3168	13478-13479	NN	denotes	J
T3172	13479-13480	-RRB-	denotes	)
T3173	13480-13481	.	denotes	.
T3174	13481-13687	sentence	denotes	Furthermore, in mutant embryos at E7.5, the expression of Dll1 in the distal streak region and in only a narrow swath of cells located laterally confirms the paucity of paraxial mesoderm (Fig. 2K,L, [24]).
T3175	13482-13493	RB	denotes	Furthermore
T3177	13493-13495	,	denotes	,
T3178	13495-13497	IN	denotes	in
T3179	13498-13504	NN	denotes	mutant
T3180	13505-13512	NNS	denotes	embryos
T3181	13513-13515	IN	denotes	at
T3182	13516-13520	NN	denotes	E7.5
T3183	13520-13522	,	denotes	,
T3184	13522-13525	DT	denotes	the
T3185	13526-13536	NN	denotes	expression
T3186	13537-13539	IN	denotes	of
T3187	13540-13544	NN	denotes	Dll1
T3188	13545-13547	IN	denotes	in
T3189	13548-13551	DT	denotes	the
T3191	13552-13558	JJ	denotes	distal
T3192	13559-13565	NN	denotes	streak
T3190	13566-13572	NN	denotes	region
T3193	13573-13576	CC	denotes	and
T3194	13577-13579	IN	denotes	in
T3195	13580-13584	RB	denotes	only
T3197	13585-13586	DT	denotes	a
T3198	13587-13593	JJ	denotes	narrow
T3196	13594-13599	NN	denotes	swath
T3199	13600-13602	IN	denotes	of
T3200	13603-13608	NNS	denotes	cells
T3201	13609-13616	VBN	denotes	located
T3202	13617-13626	RB	denotes	laterally
T3176	13627-13635	VBZ	denotes	confirms
T3203	13636-13639	DT	denotes	the
T3204	13640-13647	NN	denotes	paucity
T3205	13648-13650	IN	denotes	of
T3206	13651-13659	JJ	denotes	paraxial
T3207	13660-13668	NN	denotes	mesoderm
T3208	13669-13670	-LRB-	denotes	(
T3210	13670-13674	NN	denotes	Fig.
T3212	13675-13677	CD	denotes	2K
T3213	13677-13678	,	denotes	,
T3211	13678-13679	NN	denotes	L
T3214	13679-13681	,	denotes	,
T3215	13681-13682	-LRB-	denotes	[
T3209	13682-13684	CD	denotes	24
T3216	13684-13685	-RRB-	denotes	]
T3217	13685-13686	-RRB-	denotes	)
T3218	13686-13687	.	denotes	.
T3219	13687-13864	sentence	denotes	These results strongly suggest that anterior primitive streak formation is impaired, resulting in reduced axial and paraxial mesoderm formation and impaired neural development.
T3220	13688-13693	DT	denotes	These
T3221	13694-13701	NNS	denotes	results
T3223	13702-13710	RB	denotes	strongly
T3222	13711-13718	VBP	denotes	suggest
T3224	13719-13723	IN	denotes	that
T3226	13724-13732	JJ	denotes	anterior
T3228	13733-13742	JJ	denotes	primitive
T3227	13743-13749	NN	denotes	streak
T3229	13750-13759	NN	denotes	formation
T3225	13760-13762	VBZ	denotes	is
T3230	13763-13771	JJ	denotes	impaired
T3231	13771-13773	,	denotes	,
T3232	13773-13782	VBG	denotes	resulting
T3233	13783-13785	IN	denotes	in
T3234	13786-13793	VBN	denotes	reduced
T3236	13794-13799	JJ	denotes	axial
T3238	13800-13803	CC	denotes	and
T3239	13804-13812	JJ	denotes	paraxial
T3237	13813-13821	NN	denotes	mesoderm
T3235	13822-13831	NN	denotes	formation
T3240	13832-13835	CC	denotes	and
T3241	13836-13844	VBN	denotes	impaired
T3243	13845-13851	JJ	denotes	neural
T3242	13852-13863	NN	denotes	development
T3244	13863-13864	.	denotes	.
T3609	13866-13874	VBN	denotes	Impaired
T3611	13875-13883	JJ	denotes	regional
T3610	13884-13895	NN	denotes	restriction
T3612	13896-13898	IN	denotes	of
T3613	13899-13905	NN	denotes	growth
T3614	13906-13912	NN	denotes	factor
T3615	13913-13923	NN	denotes	expression
T3616	13924-13926	IN	denotes	in
T3617	13927-13930	DT	denotes	the
T3618	13931-13938	NN	denotes	absence
T3619	13939-13941	IN	denotes	of
T3620	13942-13952	NN	denotes	huntingtin
T3621	13952-14076	sentence	denotes	To elucidate the apparent patterning deficits, we next analyzed signaling molecules that are required for early patterning.
T3622	13953-13955	TO	denotes	To
T3623	13956-13965	VB	denotes	elucidate
T3625	13966-13969	DT	denotes	the
T3627	13970-13978	JJ	denotes	apparent
T3628	13979-13989	NN	denotes	patterning
T3626	13990-13998	NNS	denotes	deficits
T3629	13998-14000	,	denotes	,
T3630	14000-14002	PRP	denotes	we
T3631	14003-14007	RB	denotes	next
T3624	14008-14016	VBD	denotes	analyzed
T3632	14017-14026	NN	denotes	signaling
T3633	14027-14036	NNS	denotes	molecules
T3634	14037-14041	WDT	denotes	that
T3636	14042-14045	VBP	denotes	are
T3635	14046-14054	VBN	denotes	required
T3637	14055-14058	IN	denotes	for
T3638	14059-14064	JJ	denotes	early
T3639	14065-14075	NN	denotes	patterning
T3640	14075-14076	.	denotes	.
T3641	14076-14237	sentence	denotes	Nodal, a member of the Tgfβ family of secreted molecules is required for the formation and maintenance of the primitive streak and induction of the AVE [25-27].
T3642	14077-14082	NN	denotes	Nodal
T3644	14082-14084	,	denotes	,
T3645	14084-14085	DT	denotes	a
T3646	14086-14092	NN	denotes	member
T3647	14093-14095	IN	denotes	of
T3648	14096-14099	DT	denotes	the
T3650	14100-14104	NN	denotes	Tgfβ
T3649	14105-14111	NN	denotes	family
T3651	14112-14114	IN	denotes	of
T3652	14115-14123	VBN	denotes	secreted
T3653	14124-14133	NNS	denotes	molecules
T3654	14134-14136	VBZ	denotes	is
T3643	14137-14145	VBN	denotes	required
T3655	14146-14149	IN	denotes	for
T3656	14150-14153	DT	denotes	the
T3657	14154-14163	NN	denotes	formation
T3658	14164-14167	CC	denotes	and
T3659	14168-14179	NN	denotes	maintenance
T3660	14180-14182	IN	denotes	of
T3661	14183-14186	DT	denotes	the
T3663	14187-14196	JJ	denotes	primitive
T3662	14197-14203	NN	denotes	streak
T3664	14204-14207	CC	denotes	and
T3665	14208-14217	NN	denotes	induction
T3666	14218-14220	IN	denotes	of
T3667	14221-14224	DT	denotes	the
T3668	14225-14228	NN	denotes	AVE
T3669	14229-14230	-LRB-	denotes	[
T3670	14230-14232	CD	denotes	25
T3671	14232-14233	SYM	denotes	-
T3672	14233-14235	CD	denotes	27
T3673	14235-14236	-RRB-	denotes	]
T3674	14236-14237	.	denotes	.
T3675	14237-14529	sentence	denotes	Nodal is normally expressed throughout the epiblast and overlying visceral endoderm at early post implantation stages [28], but later becomes restricted to the posterior of the embryo to the site of primitive streak with asymmetrical visceral endoderm expression marking the left-right axis.
T3676	14238-14243	NN	denotes	Nodal
T3678	14244-14246	VBZ	denotes	is
T3679	14247-14255	RB	denotes	normally
T3677	14256-14265	VBN	denotes	expressed
T3680	14266-14276	IN	denotes	throughout
T3681	14277-14280	DT	denotes	the
T3682	14281-14289	NN	denotes	epiblast
T3683	14290-14293	CC	denotes	and
T3684	14294-14303	VBG	denotes	overlying
T3686	14304-14312	JJ	denotes	visceral
T3685	14313-14321	NN	denotes	endoderm
T3687	14322-14324	IN	denotes	at
T3688	14325-14330	JJ	denotes	early
T3690	14331-14335	AFX	denotes	post
T3691	14336-14348	NN	denotes	implantation
T3689	14349-14355	NNS	denotes	stages
T3692	14356-14357	-LRB-	denotes	[
T3693	14357-14359	CD	denotes	28
T3694	14359-14360	-RRB-	denotes	]
T3695	14360-14362	,	denotes	,
T3696	14362-14365	CC	denotes	but
T3697	14366-14371	RB	denotes	later
T3698	14372-14379	VBZ	denotes	becomes
T3699	14380-14390	JJ	denotes	restricted
T3700	14391-14393	IN	denotes	to
T3701	14394-14397	DT	denotes	the
T3702	14398-14407	NN	denotes	posterior
T3703	14408-14410	IN	denotes	of
T3704	14411-14414	DT	denotes	the
T3705	14415-14421	NN	denotes	embryo
T3706	14422-14424	IN	denotes	to
T3707	14425-14428	DT	denotes	the
T3708	14429-14433	NN	denotes	site
T3709	14434-14436	IN	denotes	of
T3710	14437-14446	JJ	denotes	primitive
T3711	14447-14453	NN	denotes	streak
T3712	14454-14458	IN	denotes	with
T3713	14459-14471	JJ	denotes	asymmetrical
T3715	14472-14480	JJ	denotes	visceral
T3716	14481-14489	NN	denotes	endoderm
T3714	14490-14500	NN	denotes	expression
T3717	14501-14508	VBG	denotes	marking
T3718	14509-14512	DT	denotes	the
T3720	14513-14517	JJ	denotes	left
T3722	14517-14518	SYM	denotes	-
T3721	14518-14523	JJ	denotes	right
T3719	14524-14528	NN	denotes	axis
T3723	14528-14529	.	denotes	.
T3724	14529-14582	sentence	denotes	By E7.5, Nodal expression is restricted to the node.
T3725	14530-14532	IN	denotes	By
T3727	14533-14537	NN	denotes	E7.5
T3728	14537-14539	,	denotes	,
T3729	14539-14544	NN	denotes	Nodal
T3730	14545-14555	NN	denotes	expression
T3731	14556-14558	VBZ	denotes	is
T3726	14559-14569	VBN	denotes	restricted
T3732	14570-14572	IN	denotes	to
T3733	14573-14576	DT	denotes	the
T3734	14577-14581	NN	denotes	node
T3735	14581-14582	.	denotes	.
T3736	14582-14688	sentence	denotes	Nodal expression was assessed in Hdhex4/5/Hdhex4/5 embryos heterozygous for the Ndl lac Z allele [28,29].
T3737	14583-14588	NN	denotes	Nodal
T3738	14589-14599	NN	denotes	expression
T3740	14600-14603	VBD	denotes	was
T3739	14604-14612	VBN	denotes	assessed
T3741	14613-14615	IN	denotes	in
T3742	14616-14622	NN	denotes	Hdhex4
T3744	14622-14623	HYPH	denotes	/
T3745	14623-14624	CD	denotes	5
T3746	14624-14625	HYPH	denotes	/
T3747	14625-14631	NN	denotes	Hdhex4
T3748	14631-14632	HYPH	denotes	/
T3749	14632-14633	CD	denotes	5
T3743	14634-14641	NNS	denotes	embryos
T3750	14642-14654	JJ	denotes	heterozygous
T3751	14655-14658	IN	denotes	for
T3752	14659-14662	DT	denotes	the
T3754	14663-14666	NN	denotes	Ndl
T3756	14667-14670	NN	denotes	lac
T3755	14671-14672	NN	denotes	Z
T3753	14673-14679	NN	denotes	allele
T3757	14680-14681	-LRB-	denotes	[
T3759	14681-14683	CD	denotes	28
T3760	14683-14684	,	denotes	,
T3758	14684-14686	CD	denotes	29
T3761	14686-14687	-RRB-	denotes	]
T3762	14687-14688	.	denotes	.
T3763	14688-14900	sentence	denotes	Notably, heterozygous loss of nodal does not alter the Hdhex4/5/Hdhex4/5 phenotype, as determined by morphology of Hdhex4/5/Hdhex4/5:Ndllacz/Ndl+ embryos compared with Hdhex4/5/Hdhex4/5 embryos (data not shown).
T3764	14689-14696	RB	denotes	Notably
T3766	14696-14698	,	denotes	,
T3767	14698-14710	JJ	denotes	heterozygous
T3768	14711-14715	NN	denotes	loss
T3769	14716-14718	IN	denotes	of
T3770	14719-14724	NN	denotes	nodal
T3771	14725-14729	VBZ	denotes	does
T3772	14730-14733	RB	denotes	not
T3765	14734-14739	VB	denotes	alter
T3773	14740-14743	DT	denotes	the
T3775	14744-14750	NN	denotes	Hdhex4
T3776	14750-14751	HYPH	denotes	/
T3777	14751-14752	CD	denotes	5
T3778	14752-14753	HYPH	denotes	/
T3779	14753-14759	NN	denotes	Hdhex4
T3780	14759-14760	HYPH	denotes	/
T3781	14760-14761	CD	denotes	5
T3774	14762-14771	NN	denotes	phenotype
T3782	14771-14773	,	denotes	,
T3783	14773-14775	IN	denotes	as
T3784	14776-14786	VBN	denotes	determined
T3785	14787-14789	IN	denotes	by
T3786	14790-14800	NN	denotes	morphology
T3787	14801-14803	IN	denotes	of
T3788	14804-14810	NN	denotes	Hdhex4
T3790	14810-14811	HYPH	denotes	/
T3791	14811-14812	CD	denotes	5
T3792	14812-14813	HYPH	denotes	/
T3793	14813-14819	NN	denotes	Hdhex4
T3794	14819-14820	HYPH	denotes	/
T3795	14820-14821	CD	denotes	5
T3796	14821-14822	:	denotes	:
T3797	14822-14829	NN	denotes	Ndllacz
T3798	14829-14830	HYPH	denotes	/
T3799	14830-14833	NN	denotes	Ndl
T3800	14833-14834	SYM	denotes	+
T3789	14835-14842	NNS	denotes	embryos
T3801	14843-14851	VBN	denotes	compared
T3802	14852-14856	IN	denotes	with
T3803	14857-14863	NN	denotes	Hdhex4
T3805	14863-14864	HYPH	denotes	/
T3806	14864-14865	CD	denotes	5
T3807	14865-14866	HYPH	denotes	/
T3808	14866-14872	NN	denotes	Hdhex4
T3809	14872-14873	HYPH	denotes	/
T3810	14873-14874	CD	denotes	5
T3804	14875-14882	NNS	denotes	embryos
T3811	14883-14884	-LRB-	denotes	(
T3813	14884-14888	NNS	denotes	data
T3814	14889-14892	RB	denotes	not
T3812	14893-14898	VBN	denotes	shown
T3815	14898-14899	-RRB-	denotes	)
T3816	14899-14900	.	denotes	.
T3817	14900-15184	sentence	denotes	In contrast to wild-type embryos, which exhibit tight restriction of Nodal.LacZ expression to the node, Hdhex4/5/Hdhex4/5:Ndllacz/Ndl+ embryos express Nodal.LacZ throughout the endoderm overlying the epiblast, with higher levels in the posterior in an asymmetric pattern (Fig. 3A–D).
T3818	14901-14903	IN	denotes	In
T3820	14904-14912	NN	denotes	contrast
T3821	14913-14915	IN	denotes	to
T3822	14916-14920	JJ	denotes	wild
T3824	14920-14921	HYPH	denotes	-
T3823	14921-14925	NN	denotes	type
T3825	14926-14933	NNS	denotes	embryos
T3826	14933-14935	,	denotes	,
T3827	14935-14940	WDT	denotes	which
T3828	14941-14948	VBP	denotes	exhibit
T3829	14949-14954	JJ	denotes	tight
T3830	14955-14966	NN	denotes	restriction
T3831	14967-14969	IN	denotes	of
T3832	14970-14975	NN	denotes	Nodal
T3834	14975-14976	HYPH	denotes	.
T3833	14976-14980	NN	denotes	LacZ
T3835	14981-14991	NN	denotes	expression
T3836	14992-14994	IN	denotes	to
T3837	14995-14998	DT	denotes	the
T3838	14999-15003	NN	denotes	node
T3839	15003-15005	,	denotes	,
T3840	15005-15011	NN	denotes	Hdhex4
T3842	15011-15012	HYPH	denotes	/
T3843	15012-15013	CD	denotes	5
T3844	15013-15014	HYPH	denotes	/
T3845	15014-15020	NN	denotes	Hdhex4
T3846	15020-15021	HYPH	denotes	/
T3847	15021-15022	CD	denotes	5
T3848	15022-15023	:	denotes	:
T3849	15023-15030	NN	denotes	Ndllacz
T3850	15030-15031	HYPH	denotes	/
T3851	15031-15034	NN	denotes	Ndl
T3852	15034-15035	SYM	denotes	+
T3841	15036-15043	NNS	denotes	embryos
T3819	15044-15051	VBP	denotes	express
T3853	15052-15057	NN	denotes	Nodal
T3855	15057-15058	HYPH	denotes	.
T3854	15058-15062	NN	denotes	LacZ
T3856	15063-15073	IN	denotes	throughout
T3857	15074-15077	DT	denotes	the
T3858	15078-15086	NN	denotes	endoderm
T3859	15087-15096	VBG	denotes	overlying
T3860	15097-15100	DT	denotes	the
T3861	15101-15109	NN	denotes	epiblast
T3862	15109-15111	,	denotes	,
T3863	15111-15115	IN	denotes	with
T3865	15116-15122	JJR	denotes	higher
T3864	15123-15129	NNS	denotes	levels
T3866	15130-15132	IN	denotes	in
T3867	15133-15136	DT	denotes	the
T3868	15137-15146	NN	denotes	posterior
T3869	15147-15149	IN	denotes	in
T3870	15150-15152	DT	denotes	an
T3872	15153-15163	JJ	denotes	asymmetric
T3871	15164-15171	NN	denotes	pattern
T3873	15172-15173	-LRB-	denotes	(
T3875	15173-15177	NN	denotes	Fig.
T3874	15178-15180	NN	denotes	3A
T3876	15180-15181	SYM	denotes	–
T3877	15181-15182	NN	denotes	D
T3878	15182-15183	-RRB-	denotes	)
T3879	15183-15184	.	denotes	.
T3880	15184-15296	sentence	denotes	The lack of tight restriction of nodal signal is consistent with a failure to form an organized node structure.
T3881	15185-15188	DT	denotes	The
T3882	15189-15193	NN	denotes	lack
T3884	15194-15196	IN	denotes	of
T3885	15197-15202	JJ	denotes	tight
T3886	15203-15214	NN	denotes	restriction
T3887	15215-15217	IN	denotes	of
T3888	15218-15223	NN	denotes	nodal
T3889	15224-15230	NN	denotes	signal
T3883	15231-15233	VBZ	denotes	is
T3890	15234-15244	JJ	denotes	consistent
T3891	15245-15249	IN	denotes	with
T3892	15250-15251	DT	denotes	a
T3893	15252-15259	NN	denotes	failure
T3894	15260-15262	TO	denotes	to
T3895	15263-15267	VB	denotes	form
T3896	15268-15270	DT	denotes	an
T3898	15271-15280	JJ	denotes	organized
T3899	15281-15285	NN	denotes	node
T3897	15286-15295	NN	denotes	structure
T3900	15295-15296	.	denotes	.
T3901	15296-16496	sentence	denotes	Figure 3 Impaired regional restriction of gene expression in huntingtin deficient embryos. X-gal staining of Nodal-LacZ embryos shows staining in endoderm near the node of normal embryos (A,C) but broad staining in mutant embryos (B, D), although expression is higher in the posterior. The tight node expression of Nodal in normal embryos (C) is lost in mutant embryos (D), consistent with the loss of a morphological node in the absence of huntingtin. Whole mount and in situ hybridization of E7.5 day embryos reveals that Fgf8 is detected in the proximal streak and is downregulated in cells migrating out of the streak in normal embryos (E,G). In contrast, Fgf8 remains highly expressed in mutant embryos (F,H). Transient expression of Gsc in the definitive endoderm overlying the prospective head region in normal embryos (I,K) is distinguished in other cell layers in normal embryos but remains unrestricted in mutant embryos (J,L). Earlier posterior expression of Gsc is also maintained in mutant embryos (J) while it is down-regulated in normal embryos (I). Embryos (A,B,E,F,G,H,I,K,L) are shown in a lateral view with anterior oriented to the left. Embryos (C,D) are in a posterior view. Fg
T3902	15297-16496	NN	denotes	Figure 3 Impaired regional restriction of gene expression in huntingtin deficient embryos. X-gal staining of Nodal-LacZ embryos shows staining in endoderm near the node of normal embryos (A,C) but broad staining in mutant embryos (B, D), although expression is higher in the posterior. The tight node expression of Nodal in normal embryos (C) is lost in mutant embryos (D), consistent with the loss of a morphological node in the absence of huntingtin. Whole mount and in situ hybridization of E7.5 day embryos reveals that Fgf8 is detected in the proximal streak and is downregulated in cells migrating out of the streak in normal embryos (E,G). In contrast, Fgf8 remains highly expressed in mutant embryos (F,H). Transient expression of Gsc in the definitive endoderm overlying the prospective head region in normal embryos (I,K) is distinguished in other cell layers in normal embryos but remains unrestricted in mutant embryos (J,L). Earlier posterior expression of Gsc is also maintained in mutant embryos (J) while it is down-regulated in normal embryos (I). Embryos (A,B,E,F,G,H,I,K,L) are shown in a lateral view with anterior oriented to the left. Embryos (C,D) are in a posterior view. Fg
T9177	15307-15315	VBN	denotes	Impaired
T9179	15316-15324	JJ	denotes	regional
T9178	15325-15336	NN	denotes	restriction
T9180	15337-15339	IN	denotes	of
T9181	15340-15344	NN	denotes	gene
T9182	15345-15355	NN	denotes	expression
T9183	15356-15358	IN	denotes	in
T9184	15359-15369	NN	denotes	huntingtin
T9185	15370-15379	JJ	denotes	deficient
T9186	15380-15387	NNS	denotes	embryos
T9187	15387-15388	.	denotes	.
T9188	15388-15583	sentence	denotes	X-gal staining of Nodal-LacZ embryos shows staining in endoderm near the node of normal embryos (A,C) but broad staining in mutant embryos (B, D), although expression is higher in the posterior.
T9189	15389-15390	NN	denotes	X
T9191	15390-15391	HYPH	denotes	-
T9190	15391-15394	NN	denotes	gal
T9192	15395-15403	NN	denotes	staining
T9194	15404-15406	IN	denotes	of
T9195	15407-15412	NN	denotes	Nodal
T9197	15412-15413	HYPH	denotes	-
T9196	15413-15417	NN	denotes	LacZ
T9198	15418-15425	NNS	denotes	embryos
T9193	15426-15431	VBZ	denotes	shows
T9199	15432-15440	NN	denotes	staining
T9200	15441-15443	IN	denotes	in
T9201	15444-15452	NN	denotes	endoderm
T9202	15453-15457	IN	denotes	near
T9203	15458-15461	DT	denotes	the
T9204	15462-15466	NN	denotes	node
T9205	15467-15469	IN	denotes	of
T9206	15470-15476	JJ	denotes	normal
T9207	15477-15484	NNS	denotes	embryos
T9208	15485-15486	-LRB-	denotes	(
T9210	15486-15487	NN	denotes	A
T9211	15487-15488	,	denotes	,
T9209	15488-15489	NN	denotes	C
T9212	15489-15490	-RRB-	denotes	)
T9213	15491-15494	CC	denotes	but
T9214	15495-15500	JJ	denotes	broad
T9215	15501-15509	NN	denotes	staining
T9216	15510-15512	IN	denotes	in
T9217	15513-15519	NN	denotes	mutant
T9218	15520-15527	NNS	denotes	embryos
T9219	15528-15529	-LRB-	denotes	(
T9221	15529-15530	NN	denotes	B
T9222	15530-15532	,	denotes	,
T9220	15532-15533	NN	denotes	D
T9223	15533-15534	-RRB-	denotes	)
T9224	15534-15536	,	denotes	,
T9225	15536-15544	IN	denotes	although
T9227	15545-15555	NN	denotes	expression
T9226	15556-15558	VBZ	denotes	is
T9228	15559-15565	JJR	denotes	higher
T9229	15566-15568	IN	denotes	in
T9230	15569-15572	DT	denotes	the
T9231	15573-15582	NN	denotes	posterior
T9232	15582-15583	.	denotes	.
T9233	15583-15750	sentence	denotes	The tight node expression of Nodal in normal embryos (C) is lost in mutant embryos (D), consistent with the loss of a morphological node in the absence of huntingtin.
T9234	15584-15587	DT	denotes	The
T9236	15588-15593	JJ	denotes	tight
T9237	15594-15598	NN	denotes	node
T9235	15599-15609	NN	denotes	expression
T9239	15610-15612	IN	denotes	of
T9240	15613-15618	NN	denotes	Nodal
T9241	15619-15621	IN	denotes	in
T9242	15622-15628	JJ	denotes	normal
T9243	15629-15636	NNS	denotes	embryos
T9244	15637-15638	-LRB-	denotes	(
T9245	15638-15639	NN	denotes	C
T9246	15639-15640	-RRB-	denotes	)
T9247	15641-15643	VBZ	denotes	is
T9238	15644-15648	VBN	denotes	lost
T9248	15649-15651	IN	denotes	in
T9249	15652-15658	NN	denotes	mutant
T9250	15659-15666	NNS	denotes	embryos
T9251	15667-15668	-LRB-	denotes	(
T9252	15668-15669	NN	denotes	D
T9253	15669-15670	-RRB-	denotes	)
T9254	15670-15672	,	denotes	,
T9255	15672-15682	JJ	denotes	consistent
T9256	15683-15687	IN	denotes	with
T9257	15688-15691	DT	denotes	the
T9258	15692-15696	NN	denotes	loss
T9259	15697-15699	IN	denotes	of
T9260	15700-15701	DT	denotes	a
T9262	15702-15715	JJ	denotes	morphological
T9261	15716-15720	NN	denotes	node
T9263	15721-15723	IN	denotes	in
T9264	15724-15727	DT	denotes	the
T9265	15728-15735	NN	denotes	absence
T9266	15736-15738	IN	denotes	of
T9267	15739-15749	NN	denotes	huntingtin
T9268	15749-15750	.	denotes	.
T9269	15750-15944	sentence	denotes	Whole mount and in situ hybridization of E7.5 day embryos reveals that Fgf8 is detected in the proximal streak and is downregulated in cells migrating out of the streak in normal embryos (E,G).
T9270	15751-15756	JJ	denotes	Whole
T9271	15757-15762	NN	denotes	mount
T9273	15763-15766	CC	denotes	and
T9274	15767-15769	FW	denotes	in
T9275	15770-15774	FW	denotes	situ
T9272	15775-15788	NN	denotes	hybridization
T9277	15789-15791	IN	denotes	of
T9278	15792-15796	NN	denotes	E7.5
T9279	15797-15800	NN	denotes	day
T9280	15801-15808	NNS	denotes	embryos
T9276	15809-15816	VBZ	denotes	reveals
T9281	15817-15821	IN	denotes	that
T9283	15822-15826	NN	denotes	Fgf8
T9284	15827-15829	VBZ	denotes	is
T9282	15830-15838	VBN	denotes	detected
T9285	15839-15841	IN	denotes	in
T9286	15842-15845	DT	denotes	the
T9288	15846-15854	JJ	denotes	proximal
T9287	15855-15861	NN	denotes	streak
T9289	15862-15865	CC	denotes	and
T9290	15866-15868	VBZ	denotes	is
T9291	15869-15882	VBN	denotes	downregulated
T9292	15883-15885	IN	denotes	in
T9293	15886-15891	NNS	denotes	cells
T9294	15892-15901	VBG	denotes	migrating
T9295	15902-15905	IN	denotes	out
T9296	15906-15908	IN	denotes	of
T9297	15909-15912	DT	denotes	the
T9298	15913-15919	NN	denotes	streak
T9299	15920-15922	IN	denotes	in
T9300	15923-15929	JJ	denotes	normal
T9301	15930-15937	NNS	denotes	embryos
T9302	15938-15939	-LRB-	denotes	(
T9304	15939-15940	NN	denotes	E
T9305	15940-15941	,	denotes	,
T9303	15941-15942	NN	denotes	G
T9306	15942-15943	-RRB-	denotes	)
T9307	15943-15944	.	denotes	.
T9308	15944-16012	sentence	denotes	In contrast, Fgf8 remains highly expressed in mutant embryos (F,H).
T9309	15945-15947	IN	denotes	In
T9311	15948-15956	NN	denotes	contrast
T9312	15956-15958	,	denotes	,
T9313	15958-15962	NN	denotes	Fgf8
T9310	15963-15970	VBZ	denotes	remains
T9314	15971-15977	RB	denotes	highly
T9315	15978-15987	JJ	denotes	expressed
T9316	15988-15990	IN	denotes	in
T9317	15991-15997	NN	denotes	mutant
T9318	15998-16005	NNS	denotes	embryos
T9319	16006-16007	-LRB-	denotes	(
T9321	16007-16008	NN	denotes	F
T9322	16008-16009	,	denotes	,
T9320	16009-16010	NN	denotes	H
T9323	16010-16011	-RRB-	denotes	)
T9324	16011-16012	.	denotes	.
T9325	16012-16235	sentence	denotes	Transient expression of Gsc in the definitive endoderm overlying the prospective head region in normal embryos (I,K) is distinguished in other cell layers in normal embryos but remains unrestricted in mutant embryos (J,L).
T9326	16013-16022	JJ	denotes	Transient
T9327	16023-16033	NN	denotes	expression
T9329	16034-16036	IN	denotes	of
T9330	16037-16040	NN	denotes	Gsc
T9331	16041-16043	IN	denotes	in
T9332	16044-16047	DT	denotes	the
T9334	16048-16058	JJ	denotes	definitive
T9333	16059-16067	NN	denotes	endoderm
T9335	16068-16077	VBG	denotes	overlying
T9336	16078-16081	DT	denotes	the
T9338	16082-16093	JJ	denotes	prospective
T9339	16094-16098	NN	denotes	head
T9337	16099-16105	NN	denotes	region
T9340	16106-16108	IN	denotes	in
T9341	16109-16115	JJ	denotes	normal
T9342	16116-16123	NNS	denotes	embryos
T9343	16124-16125	-LRB-	denotes	(
T9345	16125-16126	NN	denotes	I
T9346	16126-16127	,	denotes	,
T9344	16127-16128	NN	denotes	K
T9347	16128-16129	-RRB-	denotes	)
T9348	16130-16132	VBZ	denotes	is
T9328	16133-16146	VBN	denotes	distinguished
T9349	16147-16149	IN	denotes	in
T9350	16150-16155	JJ	denotes	other
T9352	16156-16160	NN	denotes	cell
T9351	16161-16167	NNS	denotes	layers
T9353	16168-16170	IN	denotes	in
T9354	16171-16177	JJ	denotes	normal
T9355	16178-16185	NNS	denotes	embryos
T9356	16186-16189	CC	denotes	but
T9357	16190-16197	VBZ	denotes	remains
T9358	16198-16210	JJ	denotes	unrestricted
T9359	16211-16213	IN	denotes	in
T9360	16214-16220	NN	denotes	mutant
T9361	16221-16228	NNS	denotes	embryos
T9362	16229-16230	-LRB-	denotes	(
T9364	16230-16231	NN	denotes	J
T9365	16231-16232	,	denotes	,
T9363	16232-16233	NN	denotes	L
T9366	16233-16234	-RRB-	denotes	)
T9367	16234-16235	.	denotes	.
T9368	16235-16362	sentence	denotes	Earlier posterior expression of Gsc is also maintained in mutant embryos (J) while it is down-regulated in normal embryos (I).
T9369	16236-16243	RBR	denotes	Earlier
T9371	16244-16253	JJ	denotes	posterior
T9370	16254-16264	NN	denotes	expression
T9373	16265-16267	IN	denotes	of
T9374	16268-16271	NN	denotes	Gsc
T9375	16272-16274	VBZ	denotes	is
T9376	16275-16279	RB	denotes	also
T9372	16280-16290	VBN	denotes	maintained
T9377	16291-16293	IN	denotes	in
T9378	16294-16300	NN	denotes	mutant
T9379	16301-16308	NNS	denotes	embryos
T9380	16309-16310	-LRB-	denotes	(
T9381	16310-16311	NN	denotes	J
T9382	16311-16312	-RRB-	denotes	)
T9383	16313-16318	IN	denotes	while
T9385	16319-16321	PRP	denotes	it
T9386	16322-16324	VBZ	denotes	is
T9387	16325-16329	RB	denotes	down
T9388	16329-16330	HYPH	denotes	-
T9384	16330-16339	VBN	denotes	regulated
T9389	16340-16342	IN	denotes	in
T9390	16343-16349	JJ	denotes	normal
T9391	16350-16357	NNS	denotes	embryos
T9392	16358-16359	-LRB-	denotes	(
T9393	16359-16360	NN	denotes	I
T9394	16360-16361	-RRB-	denotes	)
T9395	16361-16362	.	denotes	.
T9396	16362-16454	sentence	denotes	Embryos (A,B,E,F,G,H,I,K,L) are shown in a lateral view with anterior oriented to the left.
T9397	16363-16370	NNS	denotes	Embryos
T9399	16371-16372	-LRB-	denotes	(
T9401	16372-16373	NN	denotes	A
T9402	16373-16374	,	denotes	,
T9403	16374-16375	NN	denotes	B
T9404	16375-16376	,	denotes	,
T9405	16376-16377	NN	denotes	E
T9406	16377-16378	,	denotes	,
T9407	16378-16379	NN	denotes	F
T9408	16379-16380	,	denotes	,
T9409	16380-16381	NN	denotes	G
T9410	16381-16382	,	denotes	,
T9411	16382-16383	NN	denotes	H
T9412	16383-16384	,	denotes	,
T9413	16384-16385	NN	denotes	I
T9414	16385-16386	,	denotes	,
T9415	16386-16387	NN	denotes	K
T9416	16387-16388	,	denotes	,
T9400	16388-16389	NN	denotes	L
T9417	16389-16390	-RRB-	denotes	)
T9418	16391-16394	VBP	denotes	are
T9398	16395-16400	VBN	denotes	shown
T9419	16401-16403	IN	denotes	in
T9420	16404-16405	DT	denotes	a
T9422	16406-16413	JJ	denotes	lateral
T9421	16414-16418	NN	denotes	view
T9423	16419-16423	IN	denotes	with
T9425	16424-16432	NN	denotes	anterior
T9424	16433-16441	VBN	denotes	oriented
T9426	16442-16444	IN	denotes	to
T9427	16445-16448	DT	denotes	the
T9428	16449-16453	NN	denotes	left
T9429	16453-16454	.	denotes	.
T9430	16454-16493	sentence	denotes	Embryos (C,D) are in a posterior view.
T9431	16455-16462	NNS	denotes	Embryos
T9433	16463-16464	-LRB-	denotes	(
T9435	16464-16465	NN	denotes	C
T9436	16465-16466	,	denotes	,
T9434	16466-16467	NN	denotes	D
T9437	16467-16468	-RRB-	denotes	)
T9432	16469-16472	VBP	denotes	are
T9438	16473-16475	IN	denotes	in
T9439	16476-16477	DT	denotes	a
T9441	16478-16487	JJ	denotes	posterior
T9440	16488-16492	NN	denotes	view
T9442	16492-16493	.	denotes	.
T3903	16496-16497	sentence	denotes	f
T3904	16496-16497	IN	denotes	f
T3906	16497-16498	NN	denotes	8
T3905	16497-16505	sentence	denotes	8 signal
T3907	16499-16500	VBZ	denotes	s
T3908	16500-16501	IN	denotes	i
T3909	16501-16502	VBN	denotes	g
T3910	16502-16503	IN	denotes	n
T3911	16503-16504	VBG	denotes	a
T3912	16504-16505	JJ	denotes	l
T3914	16505-16507	VBZ	denotes	in
T3913	16505-16512	sentence	denotes	ing is
T3915	16507-16508	RB	denotes	g
T3916	16509-16510	IN	denotes	i
T3917	16510-16511	NNS	denotes	s
T3919	16512-16513	JJ	denotes	a
T3918	16512-16527	sentence	denotes	also essential
T3921	16513-16514	VBG	denotes	l
T3923	16514-16515	JJ	denotes	s
T3922	16515-16516	NN	denotes	o
T3924	16517-16519	NNS	denotes	es
T3925	16519-16520	VBZ	denotes	s
T3920	16520-16521	VBN	denotes	e
T3926	16521-16522	IN	denotes	n
T3927	16522-16523	JJ	denotes	t
T3928	16523-16524	IN	denotes	i
T3929	16524-16526	JJ	denotes	al
T3931	16527-16528	IN	denotes	f
T3930	16527-16538	sentence	denotes	for normal
T3932	16528-16529	RB	denotes	o
T3933	16529-16530	NNS	denotes	r
T3934	16531-16532	RB	denotes	n
T3935	16532-16537	JJ	denotes	ormal
T3937	16538-16539	NN	denotes	g
T3936	16538-16571	sentence	denotes	gastrulation in the mouse embryo.
T3938	16539-16540	NN	denotes	a
T3939	16540-16541	VBZ	denotes	s
T3940	16541-16542	JJ	denotes	t
T3941	16542-16543	IN	denotes	r
T3942	16543-16550	NN	denotes	ulation
T3943	16551-16553	IN	denotes	in
T3944	16554-16557	DT	denotes	the
T3946	16558-16563	NN	denotes	mouse
T3945	16564-16570	NN	denotes	embryo
T3947	16570-16571	.	denotes	.
T3948	16571-16644	sentence	denotes	Fgf8 is required for cell migration away from the primitive streak [30].
T3949	16572-16576	NN	denotes	Fgf8
T3951	16577-16579	VBZ	denotes	is
T3950	16580-16588	VBN	denotes	required
T3952	16589-16592	IN	denotes	for
T3953	16593-16597	NN	denotes	cell
T3954	16598-16607	NN	denotes	migration
T3955	16608-16612	RB	denotes	away
T3956	16613-16617	IN	denotes	from
T3957	16618-16621	DT	denotes	the
T3959	16622-16631	JJ	denotes	primitive
T3958	16632-16638	NN	denotes	streak
T3960	16639-16640	-LRB-	denotes	[
T3961	16640-16642	CD	denotes	30
T3962	16642-16643	-RRB-	denotes	]
T3963	16643-16644	.	denotes	.
T3964	16644-16903	sentence	denotes	Expressed just prior to streak formation in the posterior epiblast and visceral endoderm, Fgf8 is restricted to the streak mesoderm at E7.5 in a decreasing proximal-distal gradient and is downregulated in cells shortly after they exit the streak (Fig. 3E,G).
T3965	16645-16654	VBN	denotes	Expressed
T3967	16655-16659	RB	denotes	just
T3969	16660-16665	JJ	denotes	prior
T3968	16666-16668	IN	denotes	to
T3970	16669-16675	NN	denotes	streak
T3971	16676-16685	NN	denotes	formation
T3972	16686-16688	IN	denotes	in
T3973	16689-16692	DT	denotes	the
T3975	16693-16702	JJ	denotes	posterior
T3974	16703-16711	NN	denotes	epiblast
T3976	16712-16715	CC	denotes	and
T3977	16716-16724	JJ	denotes	visceral
T3978	16725-16733	NN	denotes	endoderm
T3979	16733-16735	,	denotes	,
T3980	16735-16739	NN	denotes	Fgf8
T3981	16740-16742	VBZ	denotes	is
T3966	16743-16753	VBN	denotes	restricted
T3982	16754-16756	IN	denotes	to
T3983	16757-16760	DT	denotes	the
T3985	16761-16767	NN	denotes	streak
T3984	16768-16776	NN	denotes	mesoderm
T3986	16777-16779	IN	denotes	at
T3987	16780-16784	NN	denotes	E7.5
T3988	16785-16787	IN	denotes	in
T3989	16788-16789	DT	denotes	a
T3991	16790-16800	VBG	denotes	decreasing
T3992	16801-16809	JJ	denotes	proximal
T3994	16809-16810	SYM	denotes	-
T3993	16810-16816	JJ	denotes	distal
T3990	16817-16825	NN	denotes	gradient
T3995	16826-16829	CC	denotes	and
T3996	16830-16832	VBZ	denotes	is
T3997	16833-16846	VBN	denotes	downregulated
T3998	16847-16849	IN	denotes	in
T3999	16850-16855	NNS	denotes	cells
T4000	16856-16863	RB	denotes	shortly
T4002	16864-16869	IN	denotes	after
T4003	16870-16874	PRP	denotes	they
T4001	16875-16879	VBP	denotes	exit
T4004	16880-16883	DT	denotes	the
T4005	16884-16890	NN	denotes	streak
T4006	16891-16892	-LRB-	denotes	(
T4008	16892-16896	NN	denotes	Fig.
T4009	16897-16899	CD	denotes	3E
T4010	16899-16900	,	denotes	,
T4007	16900-16901	NN	denotes	G
T4011	16901-16902	-RRB-	denotes	)
T4012	16902-16903	.	denotes	.
T4013	16903-17094	sentence	denotes	In Hdhex4/5/Hdhex4/5 embryos, Fgf8 expression is strongly expressed in the posterior region in the primitive streak and ectopically in the endoderm overlying the entire epiblast (Fig. 3F,H).
T4014	16904-16906	IN	denotes	In
T4016	16907-16913	NN	denotes	Hdhex4
T4018	16913-16914	HYPH	denotes	/
T4019	16914-16915	CD	denotes	5
T4020	16915-16916	HYPH	denotes	/
T4021	16916-16922	NN	denotes	Hdhex4
T4022	16922-16923	HYPH	denotes	/
T4023	16923-16924	CD	denotes	5
T4017	16925-16932	NNS	denotes	embryos
T4024	16932-16934	,	denotes	,
T4025	16934-16938	NN	denotes	Fgf8
T4026	16939-16949	NN	denotes	expression
T4027	16950-16952	VBZ	denotes	is
T4028	16953-16961	RB	denotes	strongly
T4015	16962-16971	VBN	denotes	expressed
T4029	16972-16974	IN	denotes	in
T4030	16975-16978	DT	denotes	the
T4032	16979-16988	JJ	denotes	posterior
T4031	16989-16995	NN	denotes	region
T4033	16996-16998	IN	denotes	in
T4034	16999-17002	DT	denotes	the
T4036	17003-17012	JJ	denotes	primitive
T4035	17013-17019	NN	denotes	streak
T4037	17020-17023	CC	denotes	and
T4038	17024-17035	RB	denotes	ectopically
T4039	17036-17038	IN	denotes	in
T4040	17039-17042	DT	denotes	the
T4041	17043-17051	NN	denotes	endoderm
T4042	17052-17061	VBG	denotes	overlying
T4043	17062-17065	DT	denotes	the
T4045	17066-17072	JJ	denotes	entire
T4044	17073-17081	NN	denotes	epiblast
T4046	17082-17083	-LRB-	denotes	(
T4048	17083-17087	NN	denotes	Fig.
T4049	17088-17090	CD	denotes	3F
T4050	17090-17091	,	denotes	,
T4047	17091-17092	NN	denotes	H
T4051	17092-17093	-RRB-	denotes	)
T4052	17093-17094	.	denotes	.
T4053	17094-17258	sentence	denotes	However, streak derivatives appear to migrate normally as evidenced by the proper anterior expression of markers such as Otx2, Hnf3β and Hesx1 anteriorly (Fig. 1).
T4054	17095-17102	RB	denotes	However
T4056	17102-17104	,	denotes	,
T4057	17104-17110	NN	denotes	streak
T4058	17111-17122	NNS	denotes	derivatives
T4055	17123-17129	VBP	denotes	appear
T4059	17130-17132	TO	denotes	to
T4060	17133-17140	VB	denotes	migrate
T4061	17141-17149	RB	denotes	normally
T4062	17150-17152	IN	denotes	as
T4063	17153-17162	VBN	denotes	evidenced
T4064	17163-17165	IN	denotes	by
T4065	17166-17169	DT	denotes	the
T4067	17170-17176	JJ	denotes	proper
T4068	17177-17185	JJ	denotes	anterior
T4066	17186-17196	NN	denotes	expression
T4069	17197-17199	IN	denotes	of
T4070	17200-17207	NNS	denotes	markers
T4071	17208-17212	JJ	denotes	such
T4072	17213-17215	IN	denotes	as
T4073	17216-17220	NN	denotes	Otx2
T4074	17220-17222	,	denotes	,
T4075	17222-17227	NN	denotes	Hnf3β
T4076	17228-17231	CC	denotes	and
T4077	17232-17237	NN	denotes	Hesx1
T4078	17238-17248	RB	denotes	anteriorly
T4079	17249-17250	-LRB-	denotes	(
T4080	17250-17254	NN	denotes	Fig.
T4081	17255-17256	CD	denotes	1
T4082	17256-17257	-RRB-	denotes	)
T4083	17257-17258	.	denotes	.
T4084	17258-17388	sentence	denotes	Therefore, mutant embryos exhibit normal migration of streak derivatives but display impaired Fgf8 repression in mutant endoderm.
T4085	17259-17268	RB	denotes	Therefore
T4087	17268-17270	,	denotes	,
T4088	17270-17276	NN	denotes	mutant
T4089	17277-17284	NNS	denotes	embryos
T4086	17285-17292	VBP	denotes	exhibit
T4090	17293-17299	JJ	denotes	normal
T4091	17300-17309	NN	denotes	migration
T4092	17310-17312	IN	denotes	of
T4093	17313-17319	NN	denotes	streak
T4094	17320-17331	NNS	denotes	derivatives
T4095	17332-17335	CC	denotes	but
T4096	17336-17343	VBP	denotes	display
T4097	17344-17352	VBN	denotes	impaired
T4099	17353-17357	NN	denotes	Fgf8
T4098	17358-17368	NN	denotes	repression
T4100	17369-17371	IN	denotes	in
T4101	17372-17378	NN	denotes	mutant
T4102	17379-17387	NN	denotes	endoderm
T4103	17387-17388	.	denotes	.
T4104	17388-17471	sentence	denotes	Hdhex4/5/Hdhex4/5 embryos also fail to restrict the expression of goosecoid (Gsc).
T4105	17389-17395	NN	denotes	Hdhex4
T4107	17395-17396	HYPH	denotes	/
T4108	17396-17397	CD	denotes	5
T4109	17397-17398	HYPH	denotes	/
T4110	17398-17404	NN	denotes	Hdhex4
T4111	17404-17405	HYPH	denotes	/
T4112	17405-17406	CD	denotes	5
T4106	17407-17414	NNS	denotes	embryos
T4114	17415-17419	RB	denotes	also
T4113	17420-17424	VBP	denotes	fail
T4115	17425-17427	TO	denotes	to
T4116	17428-17436	VB	denotes	restrict
T4117	17437-17440	DT	denotes	the
T4118	17441-17451	NN	denotes	expression
T4119	17452-17454	IN	denotes	of
T4120	17455-17464	NN	denotes	goosecoid
T4121	17465-17466	-LRB-	denotes	(
T4122	17466-17469	NN	denotes	Gsc
T4123	17469-17470	-RRB-	denotes	)
T4124	17470-17471	.	denotes	.
T4125	17471-17624	sentence	denotes	Normally, Gsc is initially expressed in the visceral endoderm and proximal, posterior streak where the primitive streak will form prior to gastrulation.
T4126	17472-17480	RB	denotes	Normally
T4128	17480-17482	,	denotes	,
T4129	17482-17485	NN	denotes	Gsc
T4130	17486-17488	VBZ	denotes	is
T4131	17489-17498	RB	denotes	initially
T4127	17499-17508	VBN	denotes	expressed
T4132	17509-17511	IN	denotes	in
T4133	17512-17515	DT	denotes	the
T4135	17516-17524	JJ	denotes	visceral
T4134	17525-17533	NN	denotes	endoderm
T4136	17534-17537	CC	denotes	and
T4137	17538-17546	JJ	denotes	proximal
T4139	17546-17548	,	denotes	,
T4140	17548-17557	JJ	denotes	posterior
T4138	17558-17564	NN	denotes	streak
T4141	17565-17570	WRB	denotes	where
T4143	17571-17574	DT	denotes	the
T4145	17575-17584	JJ	denotes	primitive
T4144	17585-17591	NN	denotes	streak
T4146	17592-17596	MD	denotes	will
T4142	17597-17601	VB	denotes	form
T4147	17602-17607	RB	denotes	prior
T4148	17608-17610	IN	denotes	to
T4149	17611-17623	NN	denotes	gastrulation
T4150	17623-17624	.	denotes	.
T4151	17624-17796	sentence	denotes	As the primitive streak forms and extends, Gsc is expressed in the distal streak, the node, and the axial mesoderm extending anteriorly from the node (Fig. 3I,K, [31,32]).
T4152	17625-17627	IN	denotes	As
T4154	17628-17631	DT	denotes	the
T4156	17632-17641	JJ	denotes	primitive
T4155	17642-17648	NN	denotes	streak
T4153	17649-17654	VBZ	denotes	forms
T4158	17655-17658	CC	denotes	and
T4159	17659-17666	VBZ	denotes	extends
T4160	17666-17668	,	denotes	,
T4161	17668-17671	NN	denotes	Gsc
T4162	17672-17674	VBZ	denotes	is
T4157	17675-17684	VBN	denotes	expressed
T4163	17685-17687	IN	denotes	in
T4164	17688-17691	DT	denotes	the
T4166	17692-17698	JJ	denotes	distal
T4165	17699-17705	NN	denotes	streak
T4167	17705-17707	,	denotes	,
T4168	17707-17710	DT	denotes	the
T4169	17711-17715	NN	denotes	node
T4170	17715-17717	,	denotes	,
T4171	17717-17720	CC	denotes	and
T4172	17721-17724	DT	denotes	the
T4174	17725-17730	JJ	denotes	axial
T4173	17731-17739	NN	denotes	mesoderm
T4175	17740-17749	VBG	denotes	extending
T4176	17750-17760	RB	denotes	anteriorly
T4177	17761-17765	IN	denotes	from
T4178	17766-17769	DT	denotes	the
T4179	17770-17774	NN	denotes	node
T4180	17775-17776	-LRB-	denotes	(
T4182	17776-17780	NN	denotes	Fig.
T4184	17781-17783	CD	denotes	3I
T4185	17783-17784	,	denotes	,
T4183	17784-17785	NN	denotes	K
T4186	17785-17787	,	denotes	,
T4187	17787-17788	-LRB-	denotes	[
T4188	17788-17790	CD	denotes	31
T4189	17790-17791	,	denotes	,
T4181	17791-17793	CD	denotes	32
T4190	17793-17794	-RRB-	denotes	]
T4191	17794-17795	-RRB-	denotes	)
T4192	17795-17796	.	denotes	.
T4193	17796-18013	sentence	denotes	However, in the mutant Hdhex4/5/Hdhex4/5 embryos, high levels of Gsc expression remain unrestricted in the endoderm overlying the entire embryo and ectopically in cells adjacent to the ectoplacental cone (Fig. 3J,L).
T4194	17797-17804	RB	denotes	However
T4196	17804-17806	,	denotes	,
T4197	17806-17808	IN	denotes	in
T4198	17809-17812	DT	denotes	the
T4200	17813-17819	NN	denotes	mutant
T4201	17820-17826	NN	denotes	Hdhex4
T4202	17826-17827	HYPH	denotes	/
T4203	17827-17828	CD	denotes	5
T4204	17828-17829	HYPH	denotes	/
T4205	17829-17835	NN	denotes	Hdhex4
T4206	17835-17836	HYPH	denotes	/
T4207	17836-17837	CD	denotes	5
T4199	17838-17845	NNS	denotes	embryos
T4208	17845-17847	,	denotes	,
T4209	17847-17851	JJ	denotes	high
T4210	17852-17858	NNS	denotes	levels
T4211	17859-17861	IN	denotes	of
T4212	17862-17865	NN	denotes	Gsc
T4213	17866-17876	NN	denotes	expression
T4195	17877-17883	VBP	denotes	remain
T4214	17884-17896	JJ	denotes	unrestricted
T4215	17897-17899	IN	denotes	in
T4216	17900-17903	DT	denotes	the
T4217	17904-17912	NN	denotes	endoderm
T4218	17913-17922	VBG	denotes	overlying
T4219	17923-17926	DT	denotes	the
T4221	17927-17933	JJ	denotes	entire
T4220	17934-17940	NN	denotes	embryo
T4222	17941-17944	CC	denotes	and
T4223	17945-17956	RB	denotes	ectopically
T4224	17957-17959	IN	denotes	in
T4225	17960-17965	NNS	denotes	cells
T4226	17966-17974	JJ	denotes	adjacent
T4227	17975-17977	IN	denotes	to
T4228	17978-17981	DT	denotes	the
T4230	17982-17995	JJ	denotes	ectoplacental
T4229	17996-18000	NN	denotes	cone
T4231	18001-18002	-LRB-	denotes	(
T4233	18002-18006	NN	denotes	Fig.
T4234	18007-18009	CD	denotes	3J
T4235	18009-18010	,	denotes	,
T4232	18010-18011	NN	denotes	L
T4236	18011-18012	-RRB-	denotes	)
T4237	18012-18013	.	denotes	.
T4238	18013-18254	sentence	denotes	These results suggest that, in contrast to proper Hnf3β regulation, Gsc remains inappropriately activated in mutant visceral and definitive endoderm, implicating huntingtin in the proper restriction of this homeodomain transcription factor.
T4239	18014-18019	DT	denotes	These
T4240	18020-18027	NNS	denotes	results
T4241	18028-18035	VBP	denotes	suggest
T4242	18036-18040	IN	denotes	that
T4244	18040-18042	,	denotes	,
T4245	18042-18044	IN	denotes	in
T4246	18045-18053	NN	denotes	contrast
T4247	18054-18056	IN	denotes	to
T4248	18057-18063	JJ	denotes	proper
T4250	18064-18069	NN	denotes	Hnf3β
T4249	18070-18080	NN	denotes	regulation
T4251	18080-18082	,	denotes	,
T4252	18082-18085	NN	denotes	Gsc
T4243	18086-18093	VBZ	denotes	remains
T4253	18094-18109	RB	denotes	inappropriately
T4254	18110-18119	JJ	denotes	activated
T4255	18120-18122	IN	denotes	in
T4256	18123-18129	NN	denotes	mutant
T4258	18130-18138	JJ	denotes	visceral
T4259	18139-18142	CC	denotes	and
T4260	18143-18153	JJ	denotes	definitive
T4257	18154-18162	NN	denotes	endoderm
T4261	18162-18164	,	denotes	,
T4262	18164-18175	VBG	denotes	implicating
T4263	18176-18186	NN	denotes	huntingtin
T4264	18187-18189	IN	denotes	in
T4265	18190-18193	DT	denotes	the
T4267	18194-18200	JJ	denotes	proper
T4266	18201-18212	NN	denotes	restriction
T4268	18213-18215	IN	denotes	of
T4269	18216-18220	DT	denotes	this
T4271	18221-18232	NN	denotes	homeodomain
T4272	18233-18246	NN	denotes	transcription
T4270	18247-18253	NN	denotes	factor
T4273	18253-18254	.	denotes	.
T4743	18256-18266	NN	denotes	Huntingtin
T4745	18267-18269	VBZ	denotes	is
T4746	18270-18273	RB	denotes	not
T4744	18274-18282	VBN	denotes	required
T4747	18283-18286	IN	denotes	for
T4748	18287-18297	NN	denotes	expression
T4749	18298-18300	IN	denotes	of
T4750	18301-18315	JJ	denotes	extraembryonic
T4752	18316-18325	NN	denotes	signaling
T4751	18326-18335	NNS	denotes	molecules
T4753	18335-18471	sentence	denotes	Previous studies of chimeric embryos suggest that huntingtin is required only in the extraembryonic tissue for proper development [16].
T4754	18336-18344	JJ	denotes	Previous
T4755	18345-18352	NNS	denotes	studies
T4757	18353-18355	IN	denotes	of
T4758	18356-18364	JJ	denotes	chimeric
T4759	18365-18372	NNS	denotes	embryos
T4756	18373-18380	VBP	denotes	suggest
T4760	18381-18385	IN	denotes	that
T4762	18386-18396	NN	denotes	huntingtin
T4763	18397-18399	VBZ	denotes	is
T4761	18400-18408	VBN	denotes	required
T4764	18409-18413	RB	denotes	only
T4765	18414-18416	IN	denotes	in
T4766	18417-18420	DT	denotes	the
T4768	18421-18435	JJ	denotes	extraembryonic
T4767	18436-18442	NN	denotes	tissue
T4769	18443-18446	IN	denotes	for
T4770	18447-18453	JJ	denotes	proper
T4771	18454-18465	NN	denotes	development
T4772	18466-18467	-LRB-	denotes	[
T4773	18467-18469	CD	denotes	16
T4774	18469-18470	-RRB-	denotes	]
T4775	18470-18471	.	denotes	.
T4776	18471-18595	sentence	denotes	Signals from the extraembryonic tissue are critical for the induction of embryonic signals and for patterning the epiblast.
T4777	18472-18479	NNS	denotes	Signals
T4779	18480-18484	IN	denotes	from
T4780	18485-18488	DT	denotes	the
T4782	18489-18503	JJ	denotes	extraembryonic
T4781	18504-18510	NN	denotes	tissue
T4778	18511-18514	VBP	denotes	are
T4783	18515-18523	JJ	denotes	critical
T4784	18524-18527	IN	denotes	for
T4785	18528-18531	DT	denotes	the
T4786	18532-18541	NN	denotes	induction
T4787	18542-18544	IN	denotes	of
T4788	18545-18554	JJ	denotes	embryonic
T4789	18555-18562	NNS	denotes	signals
T4790	18563-18566	CC	denotes	and
T4791	18567-18570	IN	denotes	for
T4792	18571-18581	VBG	denotes	patterning
T4793	18582-18585	DT	denotes	the
T4794	18586-18594	NN	denotes	epiblast
T4795	18594-18595	.	denotes	.
T4796	18595-18681	sentence	denotes	Consequently, we examined extraembryonic development in huntingtin deficient embryos.
T4797	18596-18608	RB	denotes	Consequently
T4799	18608-18610	,	denotes	,
T4800	18610-18612	PRP	denotes	we
T4798	18613-18621	VBD	denotes	examined
T4801	18622-18636	JJ	denotes	extraembryonic
T4802	18637-18648	NN	denotes	development
T4803	18649-18651	IN	denotes	in
T4804	18652-18662	NN	denotes	huntingtin
T4805	18663-18672	JJ	denotes	deficient
T4806	18673-18680	NNS	denotes	embryos
T4807	18680-18681	.	denotes	.
T4808	18681-18909	sentence	denotes	Hnf4 is a transcription factor expressed in the primitive endoderm as soon as this tissue becomes distinct and is a key regulator of visceral endoderm secreted factors such as alphafetoprotein, apolipoproteins, and transferrin.
T4809	18682-18686	NN	denotes	Hnf4
T4810	18687-18689	VBZ	denotes	is
T4811	18690-18691	DT	denotes	a
T4813	18692-18705	NN	denotes	transcription
T4812	18706-18712	NN	denotes	factor
T4814	18713-18722	VBN	denotes	expressed
T4815	18723-18725	IN	denotes	in
T4816	18726-18729	DT	denotes	the
T4818	18730-18739	JJ	denotes	primitive
T4817	18740-18748	NN	denotes	endoderm
T4819	18749-18751	RB	denotes	as
T4820	18752-18756	RB	denotes	soon
T4821	18757-18759	IN	denotes	as
T4823	18760-18764	DT	denotes	this
T4824	18765-18771	NN	denotes	tissue
T4822	18772-18779	VBZ	denotes	becomes
T4825	18780-18788	JJ	denotes	distinct
T4826	18789-18792	CC	denotes	and
T4827	18793-18795	VBZ	denotes	is
T4828	18796-18797	DT	denotes	a
T4830	18798-18801	JJ	denotes	key
T4829	18802-18811	NN	denotes	regulator
T4831	18812-18814	IN	denotes	of
T4832	18815-18823	JJ	denotes	visceral
T4833	18824-18832	NN	denotes	endoderm
T4834	18833-18841	VBN	denotes	secreted
T4835	18842-18849	NNS	denotes	factors
T4836	18850-18854	JJ	denotes	such
T4837	18855-18857	IN	denotes	as
T4838	18858-18874	NN	denotes	alphafetoprotein
T4839	18874-18876	,	denotes	,
T4840	18876-18891	NNS	denotes	apolipoproteins
T4841	18891-18893	,	denotes	,
T4842	18893-18896	CC	denotes	and
T4843	18897-18908	NN	denotes	transferrin
T4844	18908-18909	.	denotes	.
T4845	18909-18972	sentence	denotes	Inactivation of Hnf4 results in impaired gastrulation [33,34].
T4846	18910-18922	NN	denotes	Inactivation
T4848	18923-18925	IN	denotes	of
T4849	18926-18930	NN	denotes	Hnf4
T4847	18931-18938	VBZ	denotes	results
T4850	18939-18941	IN	denotes	in
T4851	18942-18950	VBN	denotes	impaired
T4852	18951-18963	NN	denotes	gastrulation
T4853	18964-18965	-LRB-	denotes	[
T4855	18965-18967	CD	denotes	33
T4856	18967-18968	,	denotes	,
T4854	18968-18970	CD	denotes	34
T4857	18970-18971	-RRB-	denotes	]
T4858	18971-18972	.	denotes	.
T4859	18972-19096	sentence	denotes	At E7.5, Hnf4 is expressed in the columnar visceral endoderm cells at the extraembryonic-ectoderm junction (Fig. 4A, [33]).
T4860	18973-18975	IN	denotes	At
T4862	18976-18980	NN	denotes	E7.5
T4863	18980-18982	,	denotes	,
T4864	18982-18986	NN	denotes	Hnf4
T4865	18987-18989	VBZ	denotes	is
T4861	18990-18999	VBN	denotes	expressed
T4866	19000-19002	IN	denotes	in
T4867	19003-19006	DT	denotes	the
T4869	19007-19015	JJ	denotes	columnar
T4871	19016-19024	JJ	denotes	visceral
T4870	19025-19033	NN	denotes	endoderm
T4868	19034-19039	NNS	denotes	cells
T4872	19040-19042	IN	denotes	at
T4873	19043-19046	DT	denotes	the
T4875	19047-19061	JJ	denotes	extraembryonic
T4877	19061-19062	HYPH	denotes	-
T4876	19062-19070	NN	denotes	ectoderm
T4874	19071-19079	NN	denotes	junction
T4878	19080-19081	-LRB-	denotes	(
T4880	19081-19085	NN	denotes	Fig.
T4881	19086-19088	CD	denotes	4A
T4882	19088-19090	,	denotes	,
T4883	19090-19091	-LRB-	denotes	[
T4879	19091-19093	CD	denotes	33
T4884	19093-19094	-RRB-	denotes	]
T4885	19094-19095	-RRB-	denotes	)
T4886	19095-19096	.	denotes	.
T4887	19096-19321	sentence	denotes	In Hdhex4/5/Hdhex4/5 embryos, consistent with normal primitive and visceral endoderm differentiation, Hnf4 expression appears normal, although the signal is stronger in mutant embryos compared to wild-type embryos (Fig. 4B).
T4888	19097-19099	IN	denotes	In
T4890	19100-19106	NN	denotes	Hdhex4
T4892	19106-19107	HYPH	denotes	/
T4893	19107-19108	CD	denotes	5
T4894	19108-19109	HYPH	denotes	/
T4895	19109-19115	NN	denotes	Hdhex4
T4896	19115-19116	HYPH	denotes	/
T4897	19116-19117	CD	denotes	5
T4891	19118-19125	NNS	denotes	embryos
T4898	19125-19127	,	denotes	,
T4899	19127-19137	JJ	denotes	consistent
T4900	19138-19142	IN	denotes	with
T4901	19143-19149	JJ	denotes	normal
T4903	19150-19159	JJ	denotes	primitive
T4904	19160-19163	CC	denotes	and
T4905	19164-19172	JJ	denotes	visceral
T4906	19173-19181	NN	denotes	endoderm
T4902	19182-19197	NN	denotes	differentiation
T4907	19197-19199	,	denotes	,
T4908	19199-19203	NN	denotes	Hnf4
T4909	19204-19214	NN	denotes	expression
T4889	19215-19222	VBZ	denotes	appears
T4910	19223-19229	JJ	denotes	normal
T4911	19229-19231	,	denotes	,
T4912	19231-19239	IN	denotes	although
T4914	19240-19243	DT	denotes	the
T4915	19244-19250	NN	denotes	signal
T4913	19251-19253	VBZ	denotes	is
T4916	19254-19262	JJR	denotes	stronger
T4917	19263-19265	IN	denotes	in
T4918	19266-19272	NN	denotes	mutant
T4919	19273-19280	NNS	denotes	embryos
T4920	19281-19289	VBN	denotes	compared
T4921	19290-19292	IN	denotes	to
T4922	19293-19297	JJ	denotes	wild
T4924	19297-19298	HYPH	denotes	-
T4923	19298-19302	NN	denotes	type
T4925	19303-19310	NNS	denotes	embryos
T4926	19311-19312	-LRB-	denotes	(
T4927	19312-19316	NN	denotes	Fig.
T4928	19317-19319	CD	denotes	4B
T4929	19319-19320	-RRB-	denotes	)
T4930	19320-19321	.	denotes	.
T4931	19321-19527	sentence	denotes	Similarly, Pem, a transcription factor expressed in proximal visceral endoderm and ectoplacental cone in wild-type embryos at E7.5, also is expressed in these tissues in the mutant embryos (Fig. 4C,D[35]).
T4932	19322-19331	RB	denotes	Similarly
T4934	19331-19333	,	denotes	,
T4935	19333-19336	NN	denotes	Pem
T4936	19336-19338	,	denotes	,
T4937	19338-19339	DT	denotes	a
T4939	19340-19353	NN	denotes	transcription
T4938	19354-19360	NN	denotes	factor
T4940	19361-19370	VBN	denotes	expressed
T4941	19371-19373	IN	denotes	in
T4942	19374-19382	JJ	denotes	proximal
T4944	19383-19391	JJ	denotes	visceral
T4943	19392-19400	NN	denotes	endoderm
T4945	19401-19404	CC	denotes	and
T4946	19405-19418	JJ	denotes	ectoplacental
T4947	19419-19423	NN	denotes	cone
T4948	19424-19426	IN	denotes	in
T4949	19427-19431	JJ	denotes	wild
T4951	19431-19432	HYPH	denotes	-
T4950	19432-19436	NN	denotes	type
T4952	19437-19444	NNS	denotes	embryos
T4953	19445-19447	IN	denotes	at
T4954	19448-19452	NN	denotes	E7.5
T4955	19452-19454	,	denotes	,
T4956	19454-19458	RB	denotes	also
T4957	19459-19461	VBZ	denotes	is
T4933	19462-19471	VBN	denotes	expressed
T4958	19472-19474	IN	denotes	in
T4959	19475-19480	DT	denotes	these
T4960	19481-19488	NNS	denotes	tissues
T4961	19489-19491	IN	denotes	in
T4962	19492-19495	DT	denotes	the
T4964	19496-19502	NN	denotes	mutant
T4963	19503-19510	NNS	denotes	embryos
T4965	19511-19512	-LRB-	denotes	(
T4967	19512-19516	NNP	denotes	Fig.
T4969	19517-19519	CD	denotes	4C
T4970	19519-19520	,	denotes	,
T4968	19520-19521	NN	denotes	D
T4971	19521-19522	-LRB-	denotes	[
T4966	19522-19524	CD	denotes	35
T4972	19524-19525	-RRB-	denotes	]
T4973	19525-19526	-RRB-	denotes	)
T4974	19526-19527	.	denotes	.
T4975	19527-19684	sentence	denotes	However, Pem expressing visceral endoderm hangs over the anterior of the mutant embryos, revealing abnormal location despite grossly normal differentiation.
T4976	19528-19535	RB	denotes	However
T4978	19535-19537	,	denotes	,
T4979	19537-19540	NN	denotes	Pem
T4980	19541-19551	VBG	denotes	expressing
T4981	19552-19560	JJ	denotes	visceral
T4982	19561-19569	NN	denotes	endoderm
T4977	19570-19575	VBZ	denotes	hangs
T4983	19576-19580	IN	denotes	over
T4984	19581-19584	DT	denotes	the
T4985	19585-19593	NN	denotes	anterior
T4986	19594-19596	IN	denotes	of
T4987	19597-19600	DT	denotes	the
T4989	19601-19607	NN	denotes	mutant
T4988	19608-19615	NNS	denotes	embryos
T4990	19615-19617	,	denotes	,
T4991	19617-19626	VBG	denotes	revealing
T4992	19627-19635	JJ	denotes	abnormal
T4993	19636-19644	NN	denotes	location
T4994	19645-19652	IN	denotes	despite
T4995	19653-19660	RB	denotes	grossly
T4996	19661-19667	JJ	denotes	normal
T4997	19668-19683	NN	denotes	differentiation
T4998	19683-19684	.	denotes	.
T4999	19684-19685	sentence	denotes
T9641	19695-19701	JJ	denotes	Normal
T9642	19702-19712	NN	denotes	expression
T9643	19713-19715	IN	denotes	of
T9644	19716-19730	JJ	denotes	extraembryonic
T9645	19731-19738	NNS	denotes	markers
T9646	19739-19741	IN	denotes	in
T9647	19742-19752	NN	denotes	huntingtin
T9648	19753-19762	JJ	denotes	deficient
T9649	19763-19770	NNS	denotes	embryos
T9650	19770-19771	.	denotes	.
T9651	19771-19927	sentence	denotes	Whole mount in situ hybridization analysis at E7.5 of markers of the extraembryonic tissues reveals grossly normal expression in the absence of huntingtin.
T9652	19772-19777	JJ	denotes	Whole
T9653	19778-19783	NN	denotes	mount
T9655	19784-19786	FW	denotes	in
T9656	19787-19791	FW	denotes	situ
T9657	19792-19805	NN	denotes	hybridization
T9654	19806-19814	NN	denotes	analysis
T9659	19815-19817	IN	denotes	at
T9660	19818-19822	NN	denotes	E7.5
T9661	19823-19825	IN	denotes	of
T9662	19826-19833	NNS	denotes	markers
T9663	19834-19836	IN	denotes	of
T9664	19837-19840	DT	denotes	the
T9666	19841-19855	JJ	denotes	extraembryonic
T9665	19856-19863	NNS	denotes	tissues
T9658	19864-19871	VBZ	denotes	reveals
T9667	19872-19879	RB	denotes	grossly
T9668	19880-19886	JJ	denotes	normal
T9669	19887-19897	NN	denotes	expression
T9670	19898-19900	IN	denotes	in
T9671	19901-19904	DT	denotes	the
T9672	19905-19912	NN	denotes	absence
T9673	19913-19915	IN	denotes	of
T9674	19916-19926	NN	denotes	huntingtin
T9675	19926-19927	.	denotes	.
T9676	19927-20093	sentence	denotes	Hnf4, expressed in the visceral endoderm at the junction of embryonic-ectoderm junction (A), is normal in mutant embryos, although the signal is slightly higher (B).
T9677	19928-19932	NN	denotes	Hnf4
T9679	19932-19934	,	denotes	,
T9680	19934-19943	VBN	denotes	expressed
T9681	19944-19946	IN	denotes	in
T9682	19947-19950	DT	denotes	the
T9684	19951-19959	JJ	denotes	visceral
T9683	19960-19968	NN	denotes	endoderm
T9685	19969-19971	IN	denotes	at
T9686	19972-19975	DT	denotes	the
T9687	19976-19984	NN	denotes	junction
T9688	19985-19987	IN	denotes	of
T9689	19988-19997	JJ	denotes	embryonic
T9691	19997-19998	HYPH	denotes	-
T9690	19998-20006	NN	denotes	ectoderm
T9692	20007-20015	NN	denotes	junction
T9693	20016-20017	-LRB-	denotes	(
T9694	20017-20018	NN	denotes	A
T9695	20018-20019	-RRB-	denotes	)
T9696	20019-20021	,	denotes	,
T9678	20021-20023	VBZ	denotes	is
T9697	20024-20030	JJ	denotes	normal
T9698	20031-20033	IN	denotes	in
T9699	20034-20040	NN	denotes	mutant
T9700	20041-20048	NNS	denotes	embryos
T9701	20048-20050	,	denotes	,
T9702	20050-20058	IN	denotes	although
T9704	20059-20062	DT	denotes	the
T9705	20063-20069	NN	denotes	signal
T9703	20070-20072	VBZ	denotes	is
T9706	20073-20081	RB	denotes	slightly
T9707	20082-20088	JJR	denotes	higher
T9708	20089-20090	-LRB-	denotes	(
T9709	20090-20091	NN	denotes	B
T9710	20091-20092	-RRB-	denotes	)
T9711	20092-20093	.	denotes	.
T9712	20093-20327	sentence	denotes	Similarly, the expression of Pem transcripts is maintained in mutant embryos (D) similar to normal embryos (C), although Pem is expressed in the abnormal lopsided overhang of visceral endoderm over the anterior of the mutant embryos.
T9713	20094-20103	RB	denotes	Similarly
T9715	20103-20105	,	denotes	,
T9716	20105-20108	DT	denotes	the
T9717	20109-20119	NN	denotes	expression
T9718	20120-20122	IN	denotes	of
T9719	20123-20126	NN	denotes	Pem
T9720	20127-20138	NNS	denotes	transcripts
T9721	20139-20141	VBZ	denotes	is
T9714	20142-20152	VBN	denotes	maintained
T9722	20153-20155	IN	denotes	in
T9723	20156-20162	NN	denotes	mutant
T9724	20163-20170	NNS	denotes	embryos
T9725	20171-20172	-LRB-	denotes	(
T9726	20172-20173	NN	denotes	D
T9727	20173-20174	-RRB-	denotes	)
T9728	20175-20182	JJ	denotes	similar
T9729	20183-20185	IN	denotes	to
T9730	20186-20192	JJ	denotes	normal
T9731	20193-20200	NNS	denotes	embryos
T9732	20201-20202	-LRB-	denotes	(
T9733	20202-20203	NN	denotes	C
T9734	20203-20204	-RRB-	denotes	)
T9735	20204-20206	,	denotes	,
T9736	20206-20214	IN	denotes	although
T9738	20215-20218	NN	denotes	Pem
T9739	20219-20221	VBZ	denotes	is
T9737	20222-20231	VBN	denotes	expressed
T9740	20232-20234	IN	denotes	in
T9741	20235-20238	DT	denotes	the
T9743	20239-20247	JJ	denotes	abnormal
T9744	20248-20256	JJ	denotes	lopsided
T9742	20257-20265	NN	denotes	overhang
T9745	20266-20268	IN	denotes	of
T9746	20269-20277	JJ	denotes	visceral
T9747	20278-20286	NN	denotes	endoderm
T9748	20287-20291	IN	denotes	over
T9749	20292-20295	DT	denotes	the
T9750	20296-20304	NN	denotes	anterior
T9751	20305-20307	IN	denotes	of
T9752	20308-20311	DT	denotes	the
T9754	20312-20318	NN	denotes	mutant
T9753	20319-20326	NNS	denotes	embryos
T9755	20326-20327	.	denotes	.
T9756	20327-20550	sentence	denotes	Expression of extraembryonic signaling molecules is unaffected by the loss of huntingtin, as evidenced by the expression of Bmp4 (E,F) in the extraembryonic ectoderm, and Lefty1 and Dkk1 (I-L) in the AVE in mutant embryos.
T9757	20328-20338	NN	denotes	Expression
T9759	20339-20341	IN	denotes	of
T9760	20342-20356	JJ	denotes	extraembryonic
T9762	20357-20366	VBG	denotes	signaling
T9761	20367-20376	NNS	denotes	molecules
T9758	20377-20379	VBZ	denotes	is
T9763	20380-20390	JJ	denotes	unaffected
T9764	20391-20393	IN	denotes	by
T9765	20394-20397	DT	denotes	the
T9766	20398-20402	NN	denotes	loss
T9767	20403-20405	IN	denotes	of
T9768	20406-20416	NN	denotes	huntingtin
T9769	20416-20418	,	denotes	,
T9770	20418-20420	IN	denotes	as
T9771	20421-20430	VBN	denotes	evidenced
T9772	20431-20433	IN	denotes	by
T9773	20434-20437	DT	denotes	the
T9774	20438-20448	NN	denotes	expression
T9775	20449-20451	IN	denotes	of
T9776	20452-20456	NN	denotes	Bmp4
T9777	20457-20458	-LRB-	denotes	(
T9779	20458-20459	NN	denotes	E
T9780	20459-20460	,	denotes	,
T9778	20460-20461	NN	denotes	F
T9781	20461-20462	-RRB-	denotes	)
T9782	20463-20465	IN	denotes	in
T9783	20466-20469	DT	denotes	the
T9785	20470-20484	JJ	denotes	extraembryonic
T9784	20485-20493	NN	denotes	ectoderm
T9786	20493-20495	,	denotes	,
T9787	20495-20498	CC	denotes	and
T9788	20499-20505	NN	denotes	Lefty1
T9789	20506-20509	CC	denotes	and
T9790	20510-20514	NN	denotes	Dkk1
T9791	20515-20516	-LRB-	denotes	(
T9792	20516-20517	NN	denotes	I
T9793	20517-20518	SYM	denotes	-
T9794	20518-20519	NN	denotes	L
T9795	20519-20520	-RRB-	denotes	)
T9796	20521-20523	IN	denotes	in
T9797	20524-20527	DT	denotes	the
T9798	20528-20531	NN	denotes	AVE
T9799	20532-20534	IN	denotes	in
T9800	20535-20541	NN	denotes	mutant
T9801	20542-20549	NNS	denotes	embryos
T9802	20549-20550	.	denotes	.
T9803	20550-20667	sentence	denotes	Bmp4 is not localized, however, to a ring of extraembryonic ectoderm in mutant embryos (F) as in normal embryos (E).
T9804	20551-20555	NN	denotes	Bmp4
T9806	20556-20558	VBZ	denotes	is
T9807	20559-20562	RB	denotes	not
T9805	20563-20572	VBN	denotes	localized
T9808	20572-20574	,	denotes	,
T9809	20574-20581	RB	denotes	however
T9810	20581-20583	,	denotes	,
T9811	20583-20585	IN	denotes	to
T9812	20586-20587	DT	denotes	a
T9813	20588-20592	NN	denotes	ring
T9814	20593-20595	IN	denotes	of
T9815	20596-20610	JJ	denotes	extraembryonic
T9816	20611-20619	NN	denotes	ectoderm
T9817	20620-20622	IN	denotes	in
T9818	20623-20629	NN	denotes	mutant
T9819	20630-20637	NNS	denotes	embryos
T9820	20638-20639	-LRB-	denotes	(
T9821	20639-20640	NN	denotes	F
T9822	20640-20641	-RRB-	denotes	)
T9823	20642-20644	IN	denotes	as
T9824	20645-20647	IN	denotes	in
T9825	20648-20654	JJ	denotes	normal
T9826	20655-20662	NNS	denotes	embryos
T9827	20663-20664	-LRB-	denotes	(
T9828	20664-20665	NN	denotes	E
T9829	20665-20666	-RRB-	denotes	)
T9830	20666-20667	.	denotes	.
T9831	20667-20852	sentence	denotes	Primitive germ cells (PCGs) are induced normally in both wild-type (G) and mutant embryos (H), suggesting the Bmp4 signaling from the extraembryonic ectoderm to the epiblast is normal.
T9832	20668-20677	JJ	denotes	Primitive
T9834	20678-20682	NN	denotes	germ
T9833	20683-20688	NNS	denotes	cells
T9836	20689-20690	-LRB-	denotes	(
T9837	20690-20694	NNS	denotes	PCGs
T9838	20694-20695	-RRB-	denotes	)
T9839	20696-20699	VBP	denotes	are
T9835	20700-20707	VBN	denotes	induced
T9840	20708-20716	RB	denotes	normally
T9841	20717-20719	IN	denotes	in
T9842	20720-20724	CC	denotes	both
T9844	20725-20729	JJ	denotes	wild
T9845	20729-20730	HYPH	denotes	-
T9843	20730-20734	NN	denotes	type
T9847	20735-20736	-LRB-	denotes	(
T9848	20736-20737	NN	denotes	G
T9849	20737-20738	-RRB-	denotes	)
T9850	20739-20742	CC	denotes	and
T9851	20743-20749	NN	denotes	mutant
T9846	20750-20757	NNS	denotes	embryos
T9852	20758-20759	-LRB-	denotes	(
T9853	20759-20760	NN	denotes	H
T9854	20760-20761	-RRB-	denotes	)
T9855	20761-20763	,	denotes	,
T9856	20763-20773	VBG	denotes	suggesting
T9857	20774-20777	DT	denotes	the
T9859	20778-20782	NN	denotes	Bmp4
T9858	20783-20792	NN	denotes	signaling
T9861	20793-20797	IN	denotes	from
T9862	20798-20801	DT	denotes	the
T9864	20802-20816	JJ	denotes	extraembryonic
T9863	20817-20825	NN	denotes	ectoderm
T9865	20826-20828	IN	denotes	to
T9866	20829-20832	DT	denotes	the
T9867	20833-20841	NN	denotes	epiblast
T9860	20842-20844	VBZ	denotes	is
T9868	20845-20851	JJ	denotes	normal
T9869	20851-20852	.	denotes	.
T9870	20852-20948	sentence	denotes	Lefty1 expression appears disorganized in mutant embryos (I) compared to wild-type embryos (J).
T9871	20853-20859	NN	denotes	Lefty1
T9872	20860-20870	NN	denotes	expression
T9873	20871-20878	VBZ	denotes	appears
T9874	20879-20891	JJ	denotes	disorganized
T9875	20892-20894	IN	denotes	in
T9876	20895-20901	NN	denotes	mutant
T9877	20902-20909	NNS	denotes	embryos
T9878	20910-20911	-LRB-	denotes	(
T9879	20911-20912	NN	denotes	I
T9880	20912-20913	-RRB-	denotes	)
T9881	20914-20922	VBN	denotes	compared
T9882	20923-20925	IN	denotes	to
T9883	20926-20930	JJ	denotes	wild
T9885	20930-20931	HYPH	denotes	-
T9884	20931-20935	NN	denotes	type
T9886	20936-20943	NNS	denotes	embryos
T9887	20944-20945	-LRB-	denotes	(
T9888	20945-20946	NN	denotes	J
T9889	20946-20947	-RRB-	denotes	)
T9890	20947-20948	.	denotes	.
T9891	20948-21072	sentence	denotes	In contrast, the anterior expression of Dkk1 in the AVE in mutant embryos (L) matches the wild-type expression pattern (K).
T9892	20949-20951	IN	denotes	In
T9894	20952-20960	NN	denotes	contrast
T9895	20960-20962	,	denotes	,
T9896	20962-20965	DT	denotes	the
T9898	20966-20974	JJ	denotes	anterior
T9897	20975-20985	NN	denotes	expression
T9899	20986-20988	IN	denotes	of
T9900	20989-20993	NN	denotes	Dkk1
T9901	20994-20996	IN	denotes	in
T9902	20997-21000	DT	denotes	the
T9903	21001-21004	NN	denotes	AVE
T9904	21005-21007	IN	denotes	in
T9905	21008-21014	NN	denotes	mutant
T9906	21015-21022	NNS	denotes	embryos
T9907	21023-21024	-LRB-	denotes	(
T9908	21024-21025	NN	denotes	L
T9909	21025-21026	-RRB-	denotes	)
T9893	21027-21034	VBZ	denotes	matches
T9910	21035-21038	DT	denotes	the
T9912	21039-21043	JJ	denotes	wild
T9914	21043-21044	HYPH	denotes	-
T9913	21044-21048	NN	denotes	type
T9915	21049-21059	NN	denotes	expression
T9911	21060-21067	NN	denotes	pattern
T9916	21068-21069	-LRB-	denotes	(
T9917	21069-21070	NN	denotes	K
T9918	21070-21071	-RRB-	denotes	)
T9919	21071-21072	.	denotes	.
T9920	21072-21201	sentence	denotes	Despite normal AVE formation, head folds fail to form in mutant embryos, even when cultured in nutrient rich media for 24 hours.
T9921	21073-21080	IN	denotes	Despite
T9923	21081-21087	JJ	denotes	normal
T9925	21088-21091	NN	denotes	AVE
T9924	21092-21101	NN	denotes	formation
T9926	21101-21103	,	denotes	,
T9927	21103-21107	NN	denotes	head
T9928	21108-21113	NNS	denotes	folds
T9922	21114-21118	VBP	denotes	fail
T9929	21119-21121	TO	denotes	to
T9930	21122-21126	VB	denotes	form
T9931	21127-21129	IN	denotes	in
T9932	21130-21136	NN	denotes	mutant
T9933	21137-21144	NNS	denotes	embryos
T9934	21144-21146	,	denotes	,
T9935	21146-21150	RB	denotes	even
T9937	21151-21155	WRB	denotes	when
T9936	21156-21164	VBN	denotes	cultured
T9938	21165-21167	IN	denotes	in
T9939	21168-21176	NN	denotes	nutrient
T9940	21177-21181	JJ	denotes	rich
T9941	21182-21187	NNS	denotes	media
T9942	21188-21191	IN	denotes	for
T9943	21192-21194	CD	denotes	24
T9944	21195-21200	NNS	denotes	hours
T9945	21200-21201	.	denotes	.
T9946	21201-21349	sentence	denotes	Wild-type E7.5 embryos, when cultured in 75% rat serum, develop somites (M), heart (white arrow, N) and head folds (blue arrow head, N) in culture.
T9947	21202-21206	JJ	denotes	Wild
T9949	21206-21207	HYPH	denotes	-
T9948	21207-21211	NN	denotes	type
T9951	21212-21216	NN	denotes	E7.5
T9950	21217-21224	NNS	denotes	embryos
T9953	21224-21226	,	denotes	,
T9954	21226-21230	WRB	denotes	when
T9955	21231-21239	VBN	denotes	cultured
T9956	21240-21242	IN	denotes	in
T9957	21243-21245	CD	denotes	75
T9958	21245-21246	NN	denotes	%
T9960	21247-21250	NN	denotes	rat
T9959	21251-21256	NN	denotes	serum
T9961	21256-21258	,	denotes	,
T9952	21258-21265	VBP	denotes	develop
T9962	21266-21273	NNS	denotes	somites
T9963	21274-21275	-LRB-	denotes	(
T9964	21275-21276	NN	denotes	M
T9965	21276-21277	-RRB-	denotes	)
T9966	21277-21279	,	denotes	,
T9967	21279-21284	NN	denotes	heart
T9968	21285-21286	-LRB-	denotes	(
T9970	21286-21291	JJ	denotes	white
T9971	21292-21297	NN	denotes	arrow
T9972	21297-21299	,	denotes	,
T9969	21299-21300	NN	denotes	N
T9973	21300-21301	-RRB-	denotes	)
T9974	21302-21305	CC	denotes	and
T9975	21306-21310	NN	denotes	head
T9976	21311-21316	NNS	denotes	folds
T9977	21317-21318	-LRB-	denotes	(
T9979	21318-21322	JJ	denotes	blue
T9981	21323-21328	NN	denotes	arrow
T9980	21329-21333	NN	denotes	head
T9982	21333-21335	,	denotes	,
T9978	21335-21336	NN	denotes	N
T9983	21336-21337	-RRB-	denotes	)
T9984	21338-21340	IN	denotes	in
T9985	21341-21348	NN	denotes	culture
T9986	21348-21349	.	denotes	.
T9987	21349-21468	sentence	denotes	In contrast, huntingtin deficient embryos continue to live in culture but do not form headfolds, heart or somites (O).
T9988	21350-21352	IN	denotes	In
T9990	21353-21361	NN	denotes	contrast
T9991	21361-21363	,	denotes	,
T9992	21363-21373	NN	denotes	huntingtin
T9993	21374-21383	JJ	denotes	deficient
T9994	21384-21391	NNS	denotes	embryos
T9989	21392-21400	VBP	denotes	continue
T9995	21401-21403	TO	denotes	to
T9996	21404-21408	VB	denotes	live
T9997	21409-21411	IN	denotes	in
T9998	21412-21419	NN	denotes	culture
T9999	21420-21423	CC	denotes	but
T10000	21424-21426	VBP	denotes	do
T10002	21427-21430	RB	denotes	not
T10001	21431-21435	VB	denotes	form
T10003	21436-21445	NNS	denotes	headfolds
T10004	21445-21447	,	denotes	,
T10005	21447-21452	NN	denotes	heart
T10006	21453-21455	CC	denotes	or
T10007	21456-21463	NNS	denotes	somites
T10008	21464-21465	-LRB-	denotes	(
T10009	21465-21466	NN	denotes	O
T10010	21466-21467	-RRB-	denotes	)
T10011	21467-21468	.	denotes	.
T10012	21468-21551	sentence	denotes	Embryos are shown in a lateral view (A-F, I-J) with anterior oriented to the left.
T10013	21469-21476	NNS	denotes	Embryos
T10015	21477-21480	VBP	denotes	are
T10014	21481-21486	VBN	denotes	shown
T10016	21487-21489	IN	denotes	in
T10017	21490-21491	DT	denotes	a
T10019	21492-21499	JJ	denotes	lateral
T10018	21500-21504	NN	denotes	view
T10020	21505-21506	-LRB-	denotes	(
T10022	21506-21507	NN	denotes	A
T10023	21507-21508	SYM	denotes	-
T10024	21508-21509	NN	denotes	F
T10025	21509-21511	,	denotes	,
T10021	21511-21512	NN	denotes	I
T10026	21512-21513	SYM	denotes	-
T10027	21513-21514	NN	denotes	J
T10028	21514-21515	-RRB-	denotes	)
T10029	21516-21520	IN	denotes	with
T10031	21521-21529	NN	denotes	anterior
T10030	21530-21538	VBN	denotes	oriented
T10032	21539-21541	IN	denotes	to
T10033	21542-21545	DT	denotes	the
T10034	21546-21550	NN	denotes	left
T10035	21550-21551	.	denotes	.
T10036	21551-21629	sentence	denotes	Embryos in (G,H,K,L) are shown in an anterior view with proximal oriented up.
T10037	21552-21559	NNS	denotes	Embryos
T10039	21560-21562	IN	denotes	in
T10040	21563-21564	-LRB-	denotes	(
T10041	21564-21565	NN	denotes	G
T10043	21565-21566	,	denotes	,
T10044	21566-21567	NN	denotes	H
T10045	21567-21568	,	denotes	,
T10046	21568-21569	NN	denotes	K
T10047	21569-21570	,	denotes	,
T10042	21570-21571	NN	denotes	L
T10048	21571-21572	-RRB-	denotes	)
T10049	21573-21576	VBP	denotes	are
T10038	21577-21582	VBN	denotes	shown
T10050	21583-21585	IN	denotes	in
T10051	21586-21588	DT	denotes	an
T10053	21589-21597	JJ	denotes	anterior
T10052	21598-21602	NN	denotes	view
T10054	21603-21607	IN	denotes	with
T10056	21608-21616	JJ	denotes	proximal
T10055	21617-21625	VBN	denotes	oriented
T10057	21626-21628	RB	denotes	up
T10058	21628-21629	.	denotes	.
T5001	21630-21632	RB	denotes	Si
T5000	21630-21648	sentence	denotes	Signals from the e
T5002	21632-21633	IN	denotes	g
T5003	21633-21634	IN	denotes	n
T5004	21634-21636	JJ	denotes	al
T5005	21636-21637	JJ	denotes	s
T5006	21638-21639	IN	denotes	f
T5007	21639-21640	JJ	denotes	r
T5008	21640-21642	NN	denotes	om
T5009	21643-21646	DT	denotes	the
T5010	21647-21648	NN	denotes	e
T5012	21648-21649	NN	denotes	x
T5011	21648-21669	sentence	denotes	xtraembryonic tissues
T5013	21649-21661	JJ	denotes	traembryonic
T5014	21662-21663	JJ	denotes	t
T5015	21663-21664	NN	denotes	i
T5016	21664-21665	IN	denotes	s
T5017	21665-21666	NN	denotes	s
T5018	21666-21667	NN	denotes	u
T5019	21667-21669	NNS	denotes	es
T5021	21669-21671	,	denotes	,
T5020	21669-21675	sentence	denotes	, incl
T5022	21671-21673	NN	denotes	in
T5023	21673-21674	JJ	denotes	c
T5024	21674-21675	JJ	denotes	l
T5026	21675-21677	VBN	denotes	ud
T5025	21675-21694	sentence	denotes	uding the anterior
T5027	21677-21679	IN	denotes	in
T5028	21679-21680	VBG	denotes	g
T5029	21681-21684	DT	denotes	the
T5030	21685-21687	NN	denotes	an
T5031	21687-21689	DT	denotes	te
T5033	21689-21691	JJ	denotes	ri
T5032	21691-21693	NN	denotes	or
T5035	21694-21695	RB	denotes	v
T5034	21694-21697	sentence	denotes	vis
T5036	21695-21696	IN	denotes	i
T5037	21696-21697	NNS	denotes	s
T5040	21697-21699	VBN	denotes	ce
T5038	21697-21710	sentence	denotes	ceral endoder
T5041	21699-21701	NN	denotes	ra
T5039	21701-21702	VBP	denotes	l
T5042	21703-21704	NNS	denotes	e
T5043	21704-21706	CC	denotes	nd
T5044	21706-21708	NNS	denotes	od
T5045	21708-21709	JJ	denotes	e
T5046	21709-21710	NN	denotes	r
T5048	21710-21711	NNS	denotes	m
T5047	21710-21713	sentence	denotes	m a
T5049	21712-21713	NNS	denotes	a
T5051	21713-21714	VBN	denotes	n
T5050	21713-21717	sentence	denotes	nd e
T5052	21714-21715	VBN	denotes	d
T5053	21716-21717	DT	denotes	e
T5056	21717-21718	JJ	denotes	x
T5054	21717-21719	sentence	denotes	xt
T5055	21718-21719	VBN	denotes	t
T5059	21719-21720	IN	denotes	r
T5057	21719-21810	sentence	denotes	raembryonic ectoderm are required for proper formation and patterning of the epiblast [17].
T5061	21720-21730	JJ	denotes	aembryonic
T5060	21731-21732	NNS	denotes	e
T5062	21732-21733	VBG	denotes	c
T5063	21733-21734	DT	denotes	t
T5065	21734-21735	JJ	denotes	o
T5064	21735-21739	NN	denotes	derm
T5066	21740-21741	CC	denotes	a
T5067	21741-21743	JJ	denotes	re
T5068	21744-21745	NN	denotes	r
T5069	21745-21746	VBP	denotes	e
T5058	21746-21752	VBN	denotes	quired
T5070	21753-21756	IN	denotes	for
T5071	21757-21763	JJ	denotes	proper
T5072	21764-21773	NN	denotes	formation
T5073	21774-21777	CC	denotes	and
T5074	21778-21788	NN	denotes	patterning
T5075	21789-21791	IN	denotes	of
T5076	21792-21795	DT	denotes	the
T5077	21796-21804	NN	denotes	epiblast
T5078	21805-21806	-LRB-	denotes	[
T5079	21806-21808	CD	denotes	17
T5080	21808-21809	-RRB-	denotes	]
T5081	21809-21810	.	denotes	.
T5082	21810-22021	sentence	denotes	Bmp4 is a signaling molecule that is first expressed uniformly throughout the extraembryonic ectoderm and subsequently is localized to a ring of extraembryonic ectoderm adjacent to the epiblast (Fig. 4E, [36]).
T5083	21811-21815	NN	denotes	Bmp4
T5084	21816-21818	VBZ	denotes	is
T5085	21819-21820	DT	denotes	a
T5087	21821-21830	NN	denotes	signaling
T5086	21831-21839	NN	denotes	molecule
T5088	21840-21844	WDT	denotes	that
T5090	21845-21847	VBZ	denotes	is
T5091	21848-21853	RB	denotes	first
T5089	21854-21863	VBN	denotes	expressed
T5092	21864-21873	RB	denotes	uniformly
T5093	21874-21884	IN	denotes	throughout
T5094	21885-21888	DT	denotes	the
T5096	21889-21903	JJ	denotes	extraembryonic
T5095	21904-21912	NN	denotes	ectoderm
T5097	21913-21916	CC	denotes	and
T5098	21917-21929	RB	denotes	subsequently
T5100	21930-21932	VBZ	denotes	is
T5099	21933-21942	VBN	denotes	localized
T5101	21943-21945	IN	denotes	to
T5102	21946-21947	DT	denotes	a
T5103	21948-21952	NN	denotes	ring
T5104	21953-21955	IN	denotes	of
T5105	21956-21970	JJ	denotes	extraembryonic
T5106	21971-21979	NN	denotes	ectoderm
T5107	21980-21988	JJ	denotes	adjacent
T5108	21989-21991	IN	denotes	to
T5109	21992-21995	DT	denotes	the
T5110	21996-22004	NN	denotes	epiblast
T5111	22005-22006	-LRB-	denotes	(
T5113	22006-22010	NN	denotes	Fig.
T5114	22011-22013	CD	denotes	4E
T5115	22013-22015	,	denotes	,
T5116	22015-22016	-LRB-	denotes	[
T5112	22016-22018	CD	denotes	36
T5117	22018-22019	-RRB-	denotes	]
T5118	22019-22020	-RRB-	denotes	)
T5119	22020-22021	.	denotes	.
T5120	22021-22177	sentence	denotes	A key factor in regulating the formation of the node and primitive streak, Bmp4 is required for patterning the embryo along the proximodistal axis [37-40].
T5121	22022-22023	DT	denotes	A
T5123	22024-22027	JJ	denotes	key
T5122	22028-22034	NN	denotes	factor
T5125	22035-22037	IN	denotes	in
T5126	22038-22048	VBG	denotes	regulating
T5127	22049-22052	DT	denotes	the
T5128	22053-22062	NN	denotes	formation
T5129	22063-22065	IN	denotes	of
T5130	22066-22069	DT	denotes	the
T5131	22070-22074	NN	denotes	node
T5132	22075-22078	CC	denotes	and
T5133	22079-22088	JJ	denotes	primitive
T5134	22089-22095	NN	denotes	streak
T5135	22095-22097	,	denotes	,
T5136	22097-22101	NN	denotes	Bmp4
T5137	22102-22104	VBZ	denotes	is
T5124	22105-22113	VBN	denotes	required
T5138	22114-22117	IN	denotes	for
T5139	22118-22128	VBG	denotes	patterning
T5140	22129-22132	DT	denotes	the
T5141	22133-22139	NN	denotes	embryo
T5142	22140-22145	IN	denotes	along
T5143	22146-22149	DT	denotes	the
T5145	22150-22163	JJ	denotes	proximodistal
T5144	22164-22168	NN	denotes	axis
T5146	22169-22170	-LRB-	denotes	[
T5147	22170-22172	CD	denotes	37
T5148	22172-22173	SYM	denotes	-
T5149	22173-22175	CD	denotes	40
T5150	22175-22176	-RRB-	denotes	]
T5151	22176-22177	.	denotes	.
T5152	22177-22519	sentence	denotes	In the absence of huntingtin, Bmp4 expression is properly maintained in the Hdhex4/5/Hdhex4/5 extraembryonic ectoderm but is also expressed throughout the extraembryonic ectoderm (Fig. 4F) in a pattern that is similar to early Bmp4 expression rather than being restricted to a ring of extraembryonic ectoderm as seen in the wild-type embryos
T5153	22178-22180	IN	denotes	In
T5155	22181-22184	DT	denotes	the
T5156	22185-22192	NN	denotes	absence
T5157	22193-22195	IN	denotes	of
T5158	22196-22206	NN	denotes	huntingtin
T5159	22206-22208	,	denotes	,
T5160	22208-22212	NN	denotes	Bmp4
T5161	22213-22223	NN	denotes	expression
T5162	22224-22226	VBZ	denotes	is
T5163	22227-22235	RB	denotes	properly
T5154	22236-22246	VBN	denotes	maintained
T5164	22247-22249	IN	denotes	in
T5165	22250-22253	DT	denotes	the
T5167	22254-22260	NN	denotes	Hdhex4
T5168	22260-22261	HYPH	denotes	/
T5169	22261-22262	CD	denotes	5
T5170	22262-22263	HYPH	denotes	/
T5171	22263-22269	NN	denotes	Hdhex4
T5172	22269-22270	HYPH	denotes	/
T5173	22270-22271	CD	denotes	5
T5174	22272-22286	JJ	denotes	extraembryonic
T5166	22287-22295	NN	denotes	ectoderm
T5175	22296-22299	CC	denotes	but
T5176	22300-22302	VBZ	denotes	is
T5178	22303-22307	RB	denotes	also
T5177	22308-22317	VBN	denotes	expressed
T5179	22318-22328	IN	denotes	throughout
T5180	22329-22332	DT	denotes	the
T5182	22333-22347	JJ	denotes	extraembryonic
T5181	22348-22356	NN	denotes	ectoderm
T5183	22357-22358	-LRB-	denotes	(
T5184	22358-22362	NN	denotes	Fig.
T5185	22363-22365	CD	denotes	4F
T5186	22365-22366	-RRB-	denotes	)
T5187	22367-22369	IN	denotes	in
T5188	22370-22371	DT	denotes	a
T5189	22372-22379	NN	denotes	pattern
T5190	22380-22384	WDT	denotes	that
T5191	22385-22387	VBZ	denotes	is
T5192	22388-22395	JJ	denotes	similar
T5193	22396-22398	IN	denotes	to
T5194	22399-22404	JJ	denotes	early
T5196	22405-22409	NN	denotes	Bmp4
T5195	22410-22420	NN	denotes	expression
T5197	22421-22427	RB	denotes	rather
T5198	22428-22432	IN	denotes	than
T5199	22433-22438	VBG	denotes	being
T5200	22439-22449	VBN	denotes	restricted
T5201	22450-22452	IN	denotes	to
T5202	22453-22454	DT	denotes	a
T5203	22455-22459	NN	denotes	ring
T5204	22460-22462	IN	denotes	of
T5205	22463-22477	JJ	denotes	extraembryonic
T5206	22478-22486	NN	denotes	ectoderm
T5207	22487-22489	IN	denotes	as
T5208	22490-22494	VBN	denotes	seen
T5209	22495-22497	IN	denotes	in
T5210	22498-22501	DT	denotes	the
T5212	22502-22506	JJ	denotes	wild
T5214	22506-22507	HYPH	denotes	-
T5213	22507-22511	NN	denotes	type
T5211	22512-22519	NNS	denotes	embryos
T5215	22519-22666	sentence	denotes	To assess Bmp4 signaling from the extraembryonic ectoderm, we evaluated primordial germ cells (PGCs), which require Bmp4 for their induction [37].
T5216	22520-22522	TO	denotes	To
T5217	22523-22529	VB	denotes	assess
T5219	22530-22534	NN	denotes	Bmp4
T5220	22535-22544	NN	denotes	signaling
T5221	22545-22549	IN	denotes	from
T5222	22550-22553	DT	denotes	the
T5224	22554-22568	JJ	denotes	extraembryonic
T5223	22569-22577	NN	denotes	ectoderm
T5225	22577-22579	,	denotes	,
T5226	22579-22581	PRP	denotes	we
T5218	22582-22591	VBD	denotes	evaluated
T5227	22592-22602	JJ	denotes	primordial
T5229	22603-22607	NN	denotes	germ
T5228	22608-22613	NNS	denotes	cells
T5230	22614-22615	-LRB-	denotes	(
T5231	22615-22619	NNS	denotes	PGCs
T5232	22619-22620	-RRB-	denotes	)
T5233	22620-22622	,	denotes	,
T5234	22622-22627	WDT	denotes	which
T5235	22628-22635	VBP	denotes	require
T5236	22636-22640	NN	denotes	Bmp4
T5237	22641-22644	IN	denotes	for
T5238	22645-22650	PRP$	denotes	their
T5239	22651-22660	NN	denotes	induction
T5240	22661-22662	-LRB-	denotes	[
T5241	22662-22664	CD	denotes	37
T5242	22664-22665	-RRB-	denotes	]
T5243	22665-22666	.	denotes	.
T5244	22666-22774	sentence	denotes	PGCs can first be detected at E7.0 and subsequently underlie the posterior portion of the primitive streak.
T5245	22667-22671	NNS	denotes	PGCs
T5247	22672-22675	MD	denotes	can
T5248	22676-22681	RB	denotes	first
T5249	22682-22684	VB	denotes	be
T5246	22685-22693	VBN	denotes	detected
T5250	22694-22696	IN	denotes	at
T5251	22697-22701	NN	denotes	E7.0
T5252	22702-22705	CC	denotes	and
T5253	22706-22718	RB	denotes	subsequently
T5254	22719-22727	VBP	denotes	underlie
T5255	22728-22731	DT	denotes	the
T5257	22732-22741	JJ	denotes	posterior
T5256	22742-22749	NN	denotes	portion
T5258	22750-22752	IN	denotes	of
T5259	22753-22756	DT	denotes	the
T5261	22757-22766	JJ	denotes	primitive
T5260	22767-22773	NN	denotes	streak
T5262	22773-22774	.	denotes	.
T5263	22774-23006	sentence	denotes	Whole mount staining of E7.5 mutant and wild-type embryos for alkaline phosphatase activity reveals that PCGs form in Hdhex4/5/Hdhex4/5 embryos, suggesting that Bmp4 signaling is functional in the absence of huntingtin (Fig. 4G,H).
T5264	22775-22780	JJ	denotes	Whole
T5265	22781-22786	NN	denotes	mount
T5266	22787-22795	NN	denotes	staining
T5268	22796-22798	IN	denotes	of
T5269	22799-22803	NN	denotes	E7.5
T5271	22804-22810	NN	denotes	mutant
T5272	22811-22814	CC	denotes	and
T5273	22815-22819	JJ	denotes	wild
T5275	22819-22820	HYPH	denotes	-
T5274	22820-22824	NN	denotes	type
T5270	22825-22832	NNS	denotes	embryos
T5276	22833-22836	IN	denotes	for
T5277	22837-22845	NN	denotes	alkaline
T5278	22846-22857	NN	denotes	phosphatase
T5279	22858-22866	NN	denotes	activity
T5267	22867-22874	VBZ	denotes	reveals
T5280	22875-22879	IN	denotes	that
T5282	22880-22884	NNS	denotes	PCGs
T5281	22885-22889	VBP	denotes	form
T5283	22890-22892	IN	denotes	in
T5284	22893-22899	NN	denotes	Hdhex4
T5286	22899-22900	HYPH	denotes	/
T5287	22900-22901	CD	denotes	5
T5288	22901-22902	HYPH	denotes	/
T5289	22902-22908	NN	denotes	Hdhex4
T5290	22908-22909	HYPH	denotes	/
T5291	22909-22910	CD	denotes	5
T5285	22911-22918	NNS	denotes	embryos
T5292	22918-22920	,	denotes	,
T5293	22920-22930	VBG	denotes	suggesting
T5294	22931-22935	IN	denotes	that
T5296	22936-22940	NN	denotes	Bmp4
T5297	22941-22950	NN	denotes	signaling
T5295	22951-22953	VBZ	denotes	is
T5298	22954-22964	JJ	denotes	functional
T5299	22965-22967	IN	denotes	in
T5300	22968-22971	DT	denotes	the
T5301	22972-22979	NN	denotes	absence
T5302	22980-22982	IN	denotes	of
T5303	22983-22993	NN	denotes	huntingtin
T5304	22994-22995	-LRB-	denotes	(
T5306	22995-22999	NN	denotes	Fig.
T5307	23000-23002	CD	denotes	4G
T5308	23002-23003	,	denotes	,
T5305	23003-23004	NN	denotes	H
T5309	23004-23005	-RRB-	denotes	)
T5310	23005-23006	.	denotes	.
T5311	23006-23127	sentence	denotes	The anterior visceral endoderm (AVE) is also an extraembryonic source of signals that are critical for early patterning.
T5312	23007-23010	DT	denotes	The
T5314	23011-23019	JJ	denotes	anterior
T5315	23020-23028	JJ	denotes	visceral
T5313	23029-23037	NN	denotes	endoderm
T5317	23038-23039	-LRB-	denotes	(
T5318	23039-23042	NN	denotes	AVE
T5319	23042-23043	-RRB-	denotes	)
T5316	23044-23046	VBZ	denotes	is
T5320	23047-23051	RB	denotes	also
T5321	23052-23054	DT	denotes	an
T5323	23055-23069	JJ	denotes	extraembryonic
T5322	23070-23076	NN	denotes	source
T5324	23077-23079	IN	denotes	of
T5325	23080-23087	NNS	denotes	signals
T5326	23088-23092	WDT	denotes	that
T5327	23093-23096	VBP	denotes	are
T5328	23097-23105	JJ	denotes	critical
T5329	23106-23109	IN	denotes	for
T5330	23110-23115	JJ	denotes	early
T5331	23116-23126	NN	denotes	patterning
T5332	23126-23127	.	denotes	.
T5333	23127-23340	sentence	denotes	Wnt and nodal antagonists, Dkk1 (mdkk-1) and Lefty1 respectively, are expressed in the AVE and are important in limiting the posteriorization of the anterior embryo by restricting Nodal and Wnt signaling [41-43].
T5334	23128-23131	NN	denotes	Wnt
T5336	23132-23135	CC	denotes	and
T5337	23136-23141	NN	denotes	nodal
T5338	23142-23153	NNS	denotes	antagonists
T5339	23153-23155	,	denotes	,
T5340	23155-23159	NN	denotes	Dkk1
T5341	23160-23161	-LRB-	denotes	(
T5342	23161-23165	NN	denotes	mdkk
T5343	23165-23166	HYPH	denotes	-
T5344	23166-23167	CD	denotes	1
T5345	23167-23168	-RRB-	denotes	)
T5346	23169-23172	CC	denotes	and
T5347	23173-23179	NN	denotes	Lefty1
T5348	23180-23192	RB	denotes	respectively
T5349	23192-23194	,	denotes	,
T5350	23194-23197	VBP	denotes	are
T5335	23198-23207	VBN	denotes	expressed
T5351	23208-23210	IN	denotes	in
T5352	23211-23214	DT	denotes	the
T5353	23215-23218	NN	denotes	AVE
T5354	23219-23222	CC	denotes	and
T5355	23223-23226	VBP	denotes	are
T5356	23227-23236	JJ	denotes	important
T5357	23237-23239	IN	denotes	in
T5358	23240-23248	VBG	denotes	limiting
T5359	23249-23252	DT	denotes	the
T5360	23253-23269	NN	denotes	posteriorization
T5361	23270-23272	IN	denotes	of
T5362	23273-23276	DT	denotes	the
T5364	23277-23285	JJ	denotes	anterior
T5363	23286-23292	NN	denotes	embryo
T5365	23293-23295	IN	denotes	by
T5366	23296-23307	VBG	denotes	restricting
T5367	23308-23313	NN	denotes	Nodal
T5369	23314-23317	CC	denotes	and
T5370	23318-23321	NN	denotes	Wnt
T5368	23322-23331	NN	denotes	signaling
T5371	23332-23333	-LRB-	denotes	[
T5372	23333-23335	CD	denotes	41
T5373	23335-23336	SYM	denotes	-
T5374	23336-23338	CD	denotes	43
T5375	23338-23339	-RRB-	denotes	]
T5376	23339-23340	.	denotes	.
T5377	23340-23485	sentence	denotes	In Hdhex4/5/Hdhex4/5 embryos, both Dkk1 (Fig. 4I,J) and Lefty1 (Fig. 4K,L) are expressed normally in the AVE as compared with wild-type embryos.
T5378	23341-23343	IN	denotes	In
T5380	23344-23350	NN	denotes	Hdhex4
T5382	23350-23351	HYPH	denotes	/
T5383	23351-23352	CD	denotes	5
T5384	23352-23353	HYPH	denotes	/
T5385	23353-23359	NN	denotes	Hdhex4
T5386	23359-23360	HYPH	denotes	/
T5387	23360-23361	CD	denotes	5
T5381	23362-23369	NNS	denotes	embryos
T5388	23369-23371	,	denotes	,
T5389	23371-23375	CC	denotes	both
T5390	23376-23380	NN	denotes	Dkk1
T5391	23381-23382	-LRB-	denotes	(
T5393	23382-23386	NN	denotes	Fig.
T5394	23387-23389	CD	denotes	4I
T5395	23389-23390	,	denotes	,
T5392	23390-23391	NN	denotes	J
T5396	23391-23392	-RRB-	denotes	)
T5397	23393-23396	CC	denotes	and
T5398	23397-23403	NN	denotes	Lefty1
T5399	23404-23405	-LRB-	denotes	(
T5401	23405-23409	NN	denotes	Fig.
T5402	23410-23412	CD	denotes	4K
T5403	23412-23413	,	denotes	,
T5400	23413-23414	NN	denotes	L
T5404	23414-23415	-RRB-	denotes	)
T5405	23416-23419	VBP	denotes	are
T5379	23420-23429	VBN	denotes	expressed
T5406	23430-23438	RB	denotes	normally
T5407	23439-23441	IN	denotes	in
T5408	23442-23445	DT	denotes	the
T5409	23446-23449	NN	denotes	AVE
T5410	23450-23452	IN	denotes	as
T5411	23453-23461	VBN	denotes	compared
T5412	23462-23466	IN	denotes	with
T5413	23467-23471	JJ	denotes	wild
T5415	23471-23472	HYPH	denotes	-
T5414	23472-23476	NN	denotes	type
T5416	23477-23484	NNS	denotes	embryos
T5417	23484-23485	.	denotes	.
T5418	23485-23712	sentence	denotes	However, Dkk-1 levels appear to be slightly increased in Hdhex4/5/Hdhex4/5 embryos, although the pattern of Dkk-1 expression remains unchanged and this increase may just reflect the same amount of expression in a smaller area.
T5419	23486-23493	RB	denotes	However
T5421	23493-23495	,	denotes	,
T5422	23495-23498	NN	denotes	Dkk
T5424	23498-23499	HYPH	denotes	-
T5425	23499-23500	CD	denotes	1
T5423	23501-23507	NNS	denotes	levels
T5420	23508-23514	VBP	denotes	appear
T5426	23515-23517	TO	denotes	to
T5428	23518-23520	VB	denotes	be
T5429	23521-23529	RB	denotes	slightly
T5427	23530-23539	VBN	denotes	increased
T5430	23540-23542	IN	denotes	in
T5431	23543-23549	NN	denotes	Hdhex4
T5433	23549-23550	HYPH	denotes	/
T5434	23550-23551	CD	denotes	5
T5435	23551-23552	HYPH	denotes	/
T5436	23552-23558	NN	denotes	Hdhex4
T5437	23558-23559	HYPH	denotes	/
T5438	23559-23560	CD	denotes	5
T5432	23561-23568	NNS	denotes	embryos
T5439	23568-23570	,	denotes	,
T5440	23570-23578	IN	denotes	although
T5442	23579-23582	DT	denotes	the
T5443	23583-23590	NN	denotes	pattern
T5444	23591-23593	IN	denotes	of
T5445	23594-23597	NN	denotes	Dkk
T5447	23597-23598	HYPH	denotes	-
T5448	23598-23599	CD	denotes	1
T5446	23600-23610	NN	denotes	expression
T5441	23611-23618	VBZ	denotes	remains
T5449	23619-23628	JJ	denotes	unchanged
T5450	23629-23632	CC	denotes	and
T5451	23633-23637	DT	denotes	this
T5452	23638-23646	NN	denotes	increase
T5454	23647-23650	MD	denotes	may
T5455	23651-23655	RB	denotes	just
T5453	23656-23663	VB	denotes	reflect
T5456	23664-23667	DT	denotes	the
T5458	23668-23672	JJ	denotes	same
T5457	23673-23679	NN	denotes	amount
T5459	23680-23682	IN	denotes	of
T5460	23683-23693	NN	denotes	expression
T5461	23694-23696	IN	denotes	in
T5462	23697-23698	DT	denotes	a
T5464	23699-23706	JJR	denotes	smaller
T5463	23707-23711	NN	denotes	area
T5465	23711-23712	.	denotes	.
T5466	23712-23913	sentence	denotes	Therefore, the ectopic expression of Nodal (Fig. 3A–D) and the decreased Wnt3a expression (Fig. 2H) in mutant embryos do not appear to be result of changes in the expression pattern of Lefty1 or Dkk1.
T5467	23713-23722	RB	denotes	Therefore
T5469	23722-23724	,	denotes	,
T5470	23724-23727	DT	denotes	the
T5472	23728-23735	JJ	denotes	ectopic
T5471	23736-23746	NN	denotes	expression
T5473	23747-23749	IN	denotes	of
T5474	23750-23755	NN	denotes	Nodal
T5475	23756-23757	-LRB-	denotes	(
T5476	23757-23761	NN	denotes	Fig.
T5477	23762-23764	CD	denotes	3A
T5478	23764-23765	SYM	denotes	–
T5479	23765-23766	NN	denotes	D
T5480	23766-23767	-RRB-	denotes	)
T5481	23768-23771	CC	denotes	and
T5482	23772-23775	DT	denotes	the
T5484	23776-23785	VBN	denotes	decreased
T5485	23786-23791	NN	denotes	Wnt3a
T5483	23792-23802	NN	denotes	expression
T5486	23803-23804	-LRB-	denotes	(
T5487	23804-23808	NN	denotes	Fig.
T5488	23809-23811	CD	denotes	2H
T5489	23811-23812	-RRB-	denotes	)
T5490	23813-23815	IN	denotes	in
T5491	23816-23822	NN	denotes	mutant
T5492	23823-23830	NNS	denotes	embryos
T5493	23831-23833	VBP	denotes	do
T5494	23834-23837	RB	denotes	not
T5468	23838-23844	VB	denotes	appear
T5495	23845-23847	TO	denotes	to
T5496	23848-23850	VB	denotes	be
T5497	23851-23857	NN	denotes	result
T5498	23858-23860	IN	denotes	of
T5499	23861-23868	NNS	denotes	changes
T5500	23869-23871	IN	denotes	in
T5501	23872-23875	DT	denotes	the
T5503	23876-23886	NN	denotes	expression
T5502	23887-23894	NN	denotes	pattern
T5504	23895-23897	IN	denotes	of
T5505	23898-23904	NN	denotes	Lefty1
T5506	23905-23907	CC	denotes	or
T5507	23908-23912	NN	denotes	Dkk1
T5508	23912-23913	.	denotes	.
T5509	23913-24024	sentence	denotes	Despite normal AVE formation and neuroectoderm induction, head folds do not form in Hdhex4/5/Hdhex4/5 embryos.
T5510	23914-23921	IN	denotes	Despite
T5512	23922-23928	JJ	denotes	normal
T5514	23929-23932	NN	denotes	AVE
T5513	23933-23942	NN	denotes	formation
T5515	23943-23946	CC	denotes	and
T5516	23947-23960	NN	denotes	neuroectoderm
T5517	23961-23970	NN	denotes	induction
T5518	23970-23972	,	denotes	,
T5519	23972-23976	NN	denotes	head
T5520	23977-23982	NNS	denotes	folds
T5521	23983-23985	VBP	denotes	do
T5522	23986-23989	RB	denotes	not
T5511	23990-23994	VB	denotes	form
T5523	23995-23997	IN	denotes	in
T5524	23998-24004	NN	denotes	Hdhex4
T5526	24004-24005	HYPH	denotes	/
T5527	24005-24006	CD	denotes	5
T5528	24006-24007	HYPH	denotes	/
T5529	24007-24013	NN	denotes	Hdhex4
T5530	24013-24014	HYPH	denotes	/
T5531	24014-24015	CD	denotes	5
T5525	24016-24023	NNS	denotes	embryos
T5532	24023-24024	.	denotes	.
T5533	24024-24220	sentence	denotes	Therefore, to determine whether mutant embryos are inherently capable of forming head folds, embryos harvested at stage E7.5 were allowed to progress in rich culture medium in vitro for 24 hours.
T5534	24025-24034	RB	denotes	Therefore
T5536	24034-24036	,	denotes	,
T5537	24036-24038	TO	denotes	to
T5538	24039-24048	VB	denotes	determine
T5539	24049-24056	IN	denotes	whether
T5541	24057-24063	NN	denotes	mutant
T5542	24064-24071	NNS	denotes	embryos
T5540	24072-24075	VBP	denotes	are
T5543	24076-24086	RB	denotes	inherently
T5544	24087-24094	JJ	denotes	capable
T5545	24095-24097	IN	denotes	of
T5546	24098-24105	VBG	denotes	forming
T5547	24106-24110	NN	denotes	head
T5548	24111-24116	NNS	denotes	folds
T5549	24116-24118	,	denotes	,
T5550	24118-24125	NNS	denotes	embryos
T5551	24126-24135	VBN	denotes	harvested
T5552	24136-24138	IN	denotes	at
T5553	24139-24144	NN	denotes	stage
T5554	24145-24149	NN	denotes	E7.5
T5555	24150-24154	VBD	denotes	were
T5535	24155-24162	VBN	denotes	allowed
T5556	24163-24165	TO	denotes	to
T5557	24166-24174	VB	denotes	progress
T5558	24175-24177	IN	denotes	in
T5559	24178-24182	JJ	denotes	rich
T5561	24183-24190	NN	denotes	culture
T5560	24191-24197	NN	denotes	medium
T5562	24198-24200	FW	denotes	in
T5563	24201-24206	FW	denotes	vitro
T5564	24207-24210	IN	denotes	for
T5565	24211-24213	CD	denotes	24
T5566	24214-24219	NNS	denotes	hours
T5567	24219-24220	.	denotes	.
T5568	24220-24303	sentence	denotes	Wild-type embryos continued to develop head folds, somites and hearts (Fig. 4M,N).
T5569	24221-24225	JJ	denotes	Wild
T5571	24225-24226	HYPH	denotes	-
T5570	24226-24230	NN	denotes	type
T5572	24231-24238	NNS	denotes	embryos
T5573	24239-24248	VBD	denotes	continued
T5574	24249-24251	TO	denotes	to
T5575	24252-24259	VB	denotes	develop
T5576	24260-24264	NN	denotes	head
T5577	24265-24270	NNS	denotes	folds
T5578	24270-24272	,	denotes	,
T5579	24272-24279	NNS	denotes	somites
T5580	24280-24283	CC	denotes	and
T5581	24284-24290	NNS	denotes	hearts
T5582	24291-24292	-LRB-	denotes	(
T5584	24292-24296	NN	denotes	Fig.
T5585	24297-24299	CD	denotes	4M
T5586	24299-24300	,	denotes	,
T5583	24300-24301	NN	denotes	N
T5587	24301-24302	-RRB-	denotes	)
T5588	24302-24303	.	denotes	.
T5589	24303-24439	sentence	denotes	In contrast, mutant stage 7.5 embryos did not develop headfolds, hearts or somites, although these embryos continued to live (Fig. 4O).
T5590	24304-24306	IN	denotes	In
T5592	24307-24315	NN	denotes	contrast
T5593	24315-24317	,	denotes	,
T5594	24317-24323	NN	denotes	mutant
T5596	24324-24329	NN	denotes	stage
T5597	24330-24333	CD	denotes	7.5
T5595	24334-24341	NNS	denotes	embryos
T5598	24342-24345	VBD	denotes	did
T5599	24346-24349	RB	denotes	not
T5591	24350-24357	VB	denotes	develop
T5600	24358-24367	NNS	denotes	headfolds
T5601	24367-24369	,	denotes	,
T5602	24369-24375	NNS	denotes	hearts
T5603	24376-24378	CC	denotes	or
T5604	24379-24386	NNS	denotes	somites
T5605	24386-24388	,	denotes	,
T5606	24388-24396	IN	denotes	although
T5608	24397-24402	DT	denotes	these
T5609	24403-24410	NNS	denotes	embryos
T5607	24411-24420	VBD	denotes	continued
T5610	24421-24423	TO	denotes	to
T5611	24424-24428	VB	denotes	live
T5612	24429-24430	-LRB-	denotes	(
T5613	24430-24434	NN	denotes	Fig.
T5614	24435-24437	CD	denotes	4O
T5615	24437-24438	-RRB-	denotes	)
T5616	24438-24439	.	denotes	.
T5617	24439-24622	sentence	denotes	These results strongly suggest that in the absence of huntingtin, embryos are unable to undergo organogenesis, even if they continue to live past E7.5 in a nutrient rich environment.
T5618	24440-24445	DT	denotes	These
T5619	24446-24453	NNS	denotes	results
T5621	24454-24462	RB	denotes	strongly
T5620	24463-24470	VBP	denotes	suggest
T5622	24471-24475	IN	denotes	that
T5624	24476-24478	IN	denotes	in
T5625	24479-24482	DT	denotes	the
T5626	24483-24490	NN	denotes	absence
T5627	24491-24493	IN	denotes	of
T5628	24494-24504	NN	denotes	huntingtin
T5629	24504-24506	,	denotes	,
T5630	24506-24513	NNS	denotes	embryos
T5623	24514-24517	VBP	denotes	are
T5631	24518-24524	JJ	denotes	unable
T5632	24525-24527	TO	denotes	to
T5633	24528-24535	VB	denotes	undergo
T5634	24536-24549	NN	denotes	organogenesis
T5635	24549-24551	,	denotes	,
T5636	24551-24555	RB	denotes	even
T5638	24556-24558	IN	denotes	if
T5639	24559-24563	PRP	denotes	they
T5637	24564-24572	VBP	denotes	continue
T5640	24573-24575	TO	denotes	to
T5641	24576-24580	VB	denotes	live
T5642	24581-24585	IN	denotes	past
T5643	24586-24590	NN	denotes	E7.5
T5644	24591-24593	IN	denotes	in
T5645	24594-24595	DT	denotes	a
T5647	24596-24604	NN	denotes	nutrient
T5648	24605-24609	JJ	denotes	rich
T5646	24610-24621	NN	denotes	environment
T5649	24621-24622	.	denotes	.
T6261	24635-24637	PRP	denotes	We
T6263	24638-24642	VBP	denotes	have
T6262	24643-24655	VBN	denotes	investigated
T6264	24656-24659	DT	denotes	the
T6266	24660-24669	JJ	denotes	embryonic
T6265	24670-24679	NNS	denotes	processes
T6267	24680-24684	WDT	denotes	that
T6268	24685-24692	VBP	denotes	require
T6269	24693-24703	NN	denotes	huntingtin
T6270	24704-24706	IN	denotes	in
T6271	24707-24712	NN	denotes	order
T6272	24713-24715	TO	denotes	to
T6274	24716-24720	RBR	denotes	more
T6275	24721-24730	RB	denotes	precisely
T6273	24731-24740	VB	denotes	delineate
T6276	24741-24751	NN	denotes	huntingtin
T6278	24751-24753	POS	denotes	's
T6279	24754-24763	JJ	denotes	essential
T6280	24764-24773	JJ	denotes	molecular
T6281	24774-24777	CC	denotes	and
T6282	24778-24786	JJ	denotes	cellular
T6277	24787-24797	NNS	denotes	activities
T6283	24798-24801	CC	denotes	and
T6284	24802-24804	TO	denotes	to
T6285	24805-24812	VB	denotes	provide
T6286	24813-24818	NNS	denotes	clues
T6287	24819-24821	IN	denotes	to
T6288	24822-24825	DT	denotes	the
T6289	24826-24835	NN	denotes	mechanism
T6290	24836-24838	IN	denotes	by
T6292	24839-24844	WDT	denotes	which
T6293	24845-24848	DT	denotes	the
T6295	24849-24857	JJ	denotes	dominant
T6296	24858-24871	NN	denotes	polyglutamine
T6297	24872-24881	NN	denotes	expansion
T6294	24882-24890	NN	denotes	mutation
T6298	24891-24893	IN	denotes	in
T6299	24894-24904	NN	denotes	huntingtin
T6291	24905-24910	VBZ	denotes	leads
T6300	24911-24913	IN	denotes	to
T6301	24914-24916	NN	denotes	HD
T6302	24917-24929	NN	denotes	pathogenesis
T6303	24929-24930	.	denotes	.
T6304	24930-25138	sentence	denotes	In pursuing the finding that huntingtin is needed only in extraembryonic tissues for normal gastrulation, our data fail to provide evidence of abnormal nutritive gene expression in Hdhex4/5/Hdhex4/5 embryos.
T6305	24931-24933	IN	denotes	In
T6307	24934-24942	VBG	denotes	pursuing
T6308	24943-24946	DT	denotes	the
T6309	24947-24954	NN	denotes	finding
T6310	24955-24959	IN	denotes	that
T6312	24960-24970	NN	denotes	huntingtin
T6313	24971-24973	VBZ	denotes	is
T6311	24974-24980	VBN	denotes	needed
T6314	24981-24985	RB	denotes	only
T6315	24986-24988	IN	denotes	in
T6316	24989-25003	JJ	denotes	extraembryonic
T6317	25004-25011	NNS	denotes	tissues
T6318	25012-25015	IN	denotes	for
T6319	25016-25022	JJ	denotes	normal
T6320	25023-25035	NN	denotes	gastrulation
T6321	25035-25037	,	denotes	,
T6322	25037-25040	PRP$	denotes	our
T6323	25041-25045	NNS	denotes	data
T6306	25046-25050	VBP	denotes	fail
T6324	25051-25053	TO	denotes	to
T6325	25054-25061	VB	denotes	provide
T6326	25062-25070	NN	denotes	evidence
T6327	25071-25073	IN	denotes	of
T6328	25074-25082	JJ	denotes	abnormal
T6330	25083-25092	NN	denotes	nutritive
T6331	25093-25097	NN	denotes	gene
T6329	25098-25108	NN	denotes	expression
T6332	25109-25111	IN	denotes	in
T6333	25112-25118	NN	denotes	Hdhex4
T6335	25118-25119	HYPH	denotes	/
T6336	25119-25120	CD	denotes	5
T6337	25120-25121	HYPH	denotes	/
T6338	25121-25127	NN	denotes	Hdhex4
T6339	25127-25128	HYPH	denotes	/
T6340	25128-25129	CD	denotes	5
T6334	25130-25137	NNS	denotes	embryos
T6341	25137-25138	.	denotes	.
T6342	25138-25410	sentence	denotes	Instead, our results reveal that huntingtin is required for normal anterior streak formation and the consequent production of paraxial mesoderm, with a previously unrecognized role for huntingtin in the proper extinction of transiently and/or dynamically expressed genes.
T6343	25139-25146	RB	denotes	Instead
T6345	25146-25148	,	denotes	,
T6346	25148-25151	PRP$	denotes	our
T6347	25152-25159	NNS	denotes	results
T6344	25160-25166	VBP	denotes	reveal
T6348	25167-25171	IN	denotes	that
T6350	25172-25182	NN	denotes	huntingtin
T6351	25183-25185	VBZ	denotes	is
T6349	25186-25194	VBN	denotes	required
T6352	25195-25198	IN	denotes	for
T6353	25199-25205	JJ	denotes	normal
T6355	25206-25214	JJ	denotes	anterior
T6356	25215-25221	NN	denotes	streak
T6354	25222-25231	NN	denotes	formation
T6357	25232-25235	CC	denotes	and
T6358	25236-25239	DT	denotes	the
T6360	25240-25250	JJ	denotes	consequent
T6359	25251-25261	NN	denotes	production
T6361	25262-25264	IN	denotes	of
T6362	25265-25273	JJ	denotes	paraxial
T6363	25274-25282	NN	denotes	mesoderm
T6364	25282-25284	,	denotes	,
T6365	25284-25288	IN	denotes	with
T6366	25289-25290	DT	denotes	a
T6368	25291-25301	RB	denotes	previously
T6369	25302-25314	JJ	denotes	unrecognized
T6367	25315-25319	NN	denotes	role
T6370	25320-25323	IN	denotes	for
T6371	25324-25334	NN	denotes	huntingtin
T6372	25335-25337	IN	denotes	in
T6373	25338-25341	DT	denotes	the
T6375	25342-25348	JJ	denotes	proper
T6374	25349-25359	NN	denotes	extinction
T6376	25360-25362	IN	denotes	of
T6377	25363-25374	RB	denotes	transiently
T6379	25375-25378	CC	denotes	and
T6380	25378-25379	HYPH	denotes	/
T6381	25379-25381	CC	denotes	or
T6382	25382-25393	RB	denotes	dynamically
T6378	25394-25403	VBN	denotes	expressed
T6383	25404-25409	NNS	denotes	genes
T6384	25409-25410	.	denotes	.
T6385	25410-25593	sentence	denotes	Indeed, the hallmark of the huntingtin deficient molecular phenotype is the impaired down-regulation of a subset of dynamically expressed genes, after the proper onset of expression.
T6386	25411-25417	RB	denotes	Indeed
T6388	25417-25419	,	denotes	,
T6389	25419-25422	DT	denotes	the
T6390	25423-25431	NN	denotes	hallmark
T6391	25432-25434	IN	denotes	of
T6392	25435-25438	DT	denotes	the
T6394	25439-25449	NN	denotes	huntingtin
T6395	25450-25459	JJ	denotes	deficient
T6396	25460-25469	JJ	denotes	molecular
T6393	25470-25479	NN	denotes	phenotype
T6387	25480-25482	VBZ	denotes	is
T6397	25483-25486	DT	denotes	the
T6399	25487-25495	VBN	denotes	impaired
T6400	25496-25500	NN	denotes	down
T6401	25500-25501	HYPH	denotes	-
T6398	25501-25511	NN	denotes	regulation
T6402	25512-25514	IN	denotes	of
T6403	25515-25516	DT	denotes	a
T6404	25517-25523	NN	denotes	subset
T6405	25524-25526	IN	denotes	of
T6406	25527-25538	RB	denotes	dynamically
T6407	25539-25548	VBN	denotes	expressed
T6408	25549-25554	NNS	denotes	genes
T6409	25554-25556	,	denotes	,
T6410	25556-25561	IN	denotes	after
T6411	25562-25565	DT	denotes	the
T6413	25566-25572	JJ	denotes	proper
T6412	25573-25578	NN	denotes	onset
T6414	25579-25581	IN	denotes	of
T6415	25582-25592	NN	denotes	expression
T6416	25592-25593	.	denotes	.
T6417	25593-25761	sentence	denotes	This phenomenon does not reflect a lack of anterior/posterior axis formation, as evidenced by the formation of the AVE anteriorly and the primitive streak posteriorly.
T6418	25594-25598	DT	denotes	This
T6419	25599-25609	NN	denotes	phenomenon
T6421	25610-25614	VBZ	denotes	does
T6422	25615-25618	RB	denotes	not
T6420	25619-25626	VB	denotes	reflect
T6423	25627-25628	DT	denotes	a
T6424	25629-25633	NN	denotes	lack
T6425	25634-25636	IN	denotes	of
T6426	25637-25645	JJ	denotes	anterior
T6428	25645-25646	HYPH	denotes	/
T6427	25646-25655	JJ	denotes	posterior
T6430	25656-25660	NN	denotes	axis
T6429	25661-25670	NN	denotes	formation
T6431	25670-25672	,	denotes	,
T6432	25672-25674	IN	denotes	as
T6433	25675-25684	VBN	denotes	evidenced
T6434	25685-25687	IN	denotes	by
T6435	25688-25691	DT	denotes	the
T6436	25692-25701	NN	denotes	formation
T6437	25702-25704	IN	denotes	of
T6438	25705-25708	DT	denotes	the
T6439	25709-25712	NN	denotes	AVE
T6440	25713-25723	RB	denotes	anteriorly
T6441	25724-25727	CC	denotes	and
T6442	25728-25731	DT	denotes	the
T6444	25732-25741	JJ	denotes	primitive
T6443	25742-25748	NN	denotes	streak
T6445	25749-25760	RB	denotes	posteriorly
T6446	25760-25761	.	denotes	.
T6447	25761-25962	sentence	denotes	Nor can it be simply explained by delayed development, as stage-specific markers, such as Wnt3a and primordial germ cells, which are detectable at E7.0 in wild-type embryos, are induced appropriately.
T6448	25762-25765	CC	denotes	Nor
T6450	25766-25769	MD	denotes	can
T6451	25770-25772	PRP	denotes	it
T6452	25773-25775	VB	denotes	be
T6453	25776-25782	RB	denotes	simply
T6449	25783-25792	VBN	denotes	explained
T6454	25793-25795	IN	denotes	by
T6455	25796-25803	VBN	denotes	delayed
T6456	25804-25815	NN	denotes	development
T6457	25815-25817	,	denotes	,
T6458	25817-25819	IN	denotes	as
T6460	25820-25825	NN	denotes	stage
T6462	25825-25826	HYPH	denotes	-
T6461	25826-25834	JJ	denotes	specific
T6463	25835-25842	NNS	denotes	markers
T6464	25842-25844	,	denotes	,
T6465	25844-25848	JJ	denotes	such
T6466	25849-25851	IN	denotes	as
T6467	25852-25857	NN	denotes	Wnt3a
T6468	25858-25861	CC	denotes	and
T6469	25862-25872	JJ	denotes	primordial
T6471	25873-25877	NN	denotes	germ
T6470	25878-25883	NNS	denotes	cells
T6472	25883-25885	,	denotes	,
T6473	25885-25890	WDT	denotes	which
T6474	25891-25894	VBP	denotes	are
T6475	25895-25905	JJ	denotes	detectable
T6476	25906-25908	IN	denotes	at
T6477	25909-25913	NN	denotes	E7.0
T6478	25914-25916	IN	denotes	in
T6479	25917-25921	JJ	denotes	wild
T6481	25921-25922	HYPH	denotes	-
T6480	25922-25926	NN	denotes	type
T6482	25927-25934	NNS	denotes	embryos
T6483	25934-25936	,	denotes	,
T6484	25936-25939	VBP	denotes	are
T6459	25940-25947	VBN	denotes	induced
T6485	25948-25961	RB	denotes	appropriately
T6486	25961-25962	.	denotes	.
T6487	25962-26116	sentence	denotes	Furthermore, the expression of T and Evx1 in the extraembryonic mesoderm of mutant embryos is not a feature of wild-type embryos, even at earlier stages.
T6488	25963-25974	RB	denotes	Furthermore
T6490	25974-25976	,	denotes	,
T6491	25976-25979	DT	denotes	the
T6492	25980-25990	NN	denotes	expression
T6493	25991-25993	IN	denotes	of
T6494	25994-25995	NN	denotes	T
T6495	25996-25999	CC	denotes	and
T6496	26000-26004	NN	denotes	Evx1
T6497	26005-26007	IN	denotes	in
T6498	26008-26011	DT	denotes	the
T6500	26012-26026	JJ	denotes	extraembryonic
T6499	26027-26035	NN	denotes	mesoderm
T6501	26036-26038	IN	denotes	of
T6502	26039-26045	NN	denotes	mutant
T6503	26046-26053	NNS	denotes	embryos
T6489	26054-26056	VBZ	denotes	is
T6504	26057-26060	RB	denotes	not
T6505	26061-26062	DT	denotes	a
T6506	26063-26070	NN	denotes	feature
T6507	26071-26073	IN	denotes	of
T6508	26074-26078	JJ	denotes	wild
T6510	26078-26079	HYPH	denotes	-
T6509	26079-26083	NN	denotes	type
T6511	26084-26091	NNS	denotes	embryos
T6512	26091-26093	,	denotes	,
T6513	26093-26097	RB	denotes	even
T6514	26098-26100	IN	denotes	at
T6515	26101-26108	JJR	denotes	earlier
T6516	26109-26115	NNS	denotes	stages
T6517	26115-26116	.	denotes	.
T6518	26116-26328	sentence	denotes	This strongly suggests that in huntingtin deficient embryos, the migration of the distal streak derivatives to the extraembryonic mesoderm occurs normally but that the down-regulation of these genes is impaired.
T6519	26117-26121	DT	denotes	This
T6521	26122-26130	RB	denotes	strongly
T6520	26131-26139	VBZ	denotes	suggests
T6522	26140-26144	IN	denotes	that
T6524	26145-26147	IN	denotes	in
T6525	26148-26158	NN	denotes	huntingtin
T6526	26159-26168	JJ	denotes	deficient
T6527	26169-26176	NNS	denotes	embryos
T6528	26176-26178	,	denotes	,
T6529	26178-26181	DT	denotes	the
T6530	26182-26191	NN	denotes	migration
T6531	26192-26194	IN	denotes	of
T6532	26195-26198	DT	denotes	the
T6534	26199-26205	JJ	denotes	distal
T6535	26206-26212	NN	denotes	streak
T6533	26213-26224	NNS	denotes	derivatives
T6536	26225-26227	IN	denotes	to
T6537	26228-26231	DT	denotes	the
T6539	26232-26246	JJ	denotes	extraembryonic
T6538	26247-26255	NN	denotes	mesoderm
T6523	26256-26262	VBZ	denotes	occurs
T6540	26263-26271	RB	denotes	normally
T6541	26272-26275	CC	denotes	but
T6542	26276-26280	IN	denotes	that
T6544	26281-26284	DT	denotes	the
T6546	26285-26289	NN	denotes	down
T6547	26289-26290	HYPH	denotes	-
T6545	26290-26300	NN	denotes	regulation
T6548	26301-26303	IN	denotes	of
T6549	26304-26309	DT	denotes	these
T6550	26310-26315	NNS	denotes	genes
T6551	26316-26318	VBZ	denotes	is
T6543	26319-26327	VBN	denotes	impaired
T6552	26327-26328	.	denotes	.
T6553	26328-26465	sentence	denotes	This impairment may also explain the failure of huntingtin deficient embryos to properly restrict the expression of Fgf8, Nodal and Gsc.
T6554	26329-26333	DT	denotes	This
T6555	26334-26344	NN	denotes	impairment
T6557	26345-26348	MD	denotes	may
T6558	26349-26353	RB	denotes	also
T6556	26354-26361	VB	denotes	explain
T6559	26362-26365	DT	denotes	the
T6560	26366-26373	NN	denotes	failure
T6561	26374-26376	IN	denotes	of
T6562	26377-26387	NN	denotes	huntingtin
T6563	26388-26397	JJ	denotes	deficient
T6564	26398-26405	NNS	denotes	embryos
T6565	26406-26408	TO	denotes	to
T6567	26409-26417	RB	denotes	properly
T6566	26418-26426	VB	denotes	restrict
T6568	26427-26430	DT	denotes	the
T6569	26431-26441	NN	denotes	expression
T6570	26442-26444	IN	denotes	of
T6571	26445-26449	NN	denotes	Fgf8
T6572	26449-26451	,	denotes	,
T6573	26451-26456	NN	denotes	Nodal
T6574	26457-26460	CC	denotes	and
T6575	26461-26464	NN	denotes	Gsc
T6576	26464-26465	.	denotes	.
T6577	26465-26679	sentence	denotes	Thus, huntingtin may play a direct role in the transcriptional regulation, or mRNA stability of these genes or it may act indirectly by intersecting with other pathways that regulate the expression of these genes.
T6578	26466-26470	RB	denotes	Thus
T6580	26470-26472	,	denotes	,
T6581	26472-26482	NN	denotes	huntingtin
T6582	26483-26486	MD	denotes	may
T6579	26487-26491	VB	denotes	play
T6583	26492-26493	DT	denotes	a
T6585	26494-26500	JJ	denotes	direct
T6584	26501-26505	NN	denotes	role
T6586	26506-26508	IN	denotes	in
T6587	26509-26512	DT	denotes	the
T6589	26513-26528	JJ	denotes	transcriptional
T6588	26529-26539	NN	denotes	regulation
T6590	26539-26541	,	denotes	,
T6591	26541-26543	CC	denotes	or
T6592	26544-26548	NN	denotes	mRNA
T6593	26549-26558	NN	denotes	stability
T6594	26559-26561	IN	denotes	of
T6595	26562-26567	DT	denotes	these
T6596	26568-26573	NNS	denotes	genes
T6597	26574-26576	CC	denotes	or
T6598	26577-26579	PRP	denotes	it
T6600	26580-26583	MD	denotes	may
T6599	26584-26587	VB	denotes	act
T6601	26588-26598	RB	denotes	indirectly
T6602	26599-26601	IN	denotes	by
T6603	26602-26614	VBG	denotes	intersecting
T6604	26615-26619	IN	denotes	with
T6605	26620-26625	JJ	denotes	other
T6606	26626-26634	NNS	denotes	pathways
T6607	26635-26639	WDT	denotes	that
T6608	26640-26648	VBP	denotes	regulate
T6609	26649-26652	DT	denotes	the
T6610	26653-26663	NN	denotes	expression
T6611	26664-26666	IN	denotes	of
T6612	26667-26672	DT	denotes	these
T6613	26673-26678	NNS	denotes	genes
T6614	26678-26679	.	denotes	.
T6615	26679-26933	sentence	denotes	The requirement for huntingtin in the extraembryonic tissues had prompted us to test whether impaired extraembryonic signals might be responsible for the dysregulation of gene expression within the epiblast that is observed in Hdhex4/5/Hdhex4/5 embryos.
T6616	26680-26683	DT	denotes	The
T6617	26684-26695	NN	denotes	requirement
T6619	26696-26699	IN	denotes	for
T6620	26700-26710	NN	denotes	huntingtin
T6621	26711-26713	IN	denotes	in
T6622	26714-26717	DT	denotes	the
T6624	26718-26732	JJ	denotes	extraembryonic
T6623	26733-26740	NNS	denotes	tissues
T6625	26741-26744	VBD	denotes	had
T6618	26745-26753	VBN	denotes	prompted
T6626	26754-26756	PRP	denotes	us
T6627	26757-26759	TO	denotes	to
T6628	26760-26764	VB	denotes	test
T6629	26765-26772	IN	denotes	whether
T6631	26773-26781	VBN	denotes	impaired
T6633	26782-26796	JJ	denotes	extraembryonic
T6632	26797-26804	NNS	denotes	signals
T6634	26805-26810	MD	denotes	might
T6630	26811-26813	VB	denotes	be
T6635	26814-26825	JJ	denotes	responsible
T6636	26826-26829	IN	denotes	for
T6637	26830-26833	DT	denotes	the
T6638	26834-26847	NN	denotes	dysregulation
T6639	26848-26850	IN	denotes	of
T6640	26851-26855	NN	denotes	gene
T6641	26856-26866	NN	denotes	expression
T6642	26867-26873	IN	denotes	within
T6643	26874-26877	DT	denotes	the
T6644	26878-26886	NN	denotes	epiblast
T6645	26887-26891	WDT	denotes	that
T6647	26892-26894	VBZ	denotes	is
T6646	26895-26903	VBN	denotes	observed
T6648	26904-26906	IN	denotes	in
T6649	26907-26913	NN	denotes	Hdhex4
T6651	26913-26914	HYPH	denotes	/
T6652	26914-26915	CD	denotes	5
T6653	26915-26916	HYPH	denotes	/
T6654	26916-26922	NN	denotes	Hdhex4
T6655	26922-26923	HYPH	denotes	/
T6656	26923-26924	CD	denotes	5
T6650	26925-26932	NNS	denotes	embryos
T6657	26932-26933	.	denotes	.
T6658	26933-27409	sentence	denotes	Extraembryonic development in Hdhex4/5/Hdhex4/5 embryos is associated with mildly elevated levels expression of Hnf4 in the primitive endoderm and Pem in the lopsided anterior chorion but the expression of other known signals, such as Bmp4 from the extraembryonic ectoderm, and Dkk1 and Lefty1 from the AVE, appear to be normal, although the slight increase in Dkk-1 expression in Hdhex4/5/Hdhex4/5 embryos suggests that further investigation into Wnt signaling is warranted.
T6659	26934-26948	JJ	denotes	Extraembryonic
T6660	26949-26960	NN	denotes	development
T6662	26961-26963	IN	denotes	in
T6663	26964-26970	NN	denotes	Hdhex4
T6665	26970-26971	HYPH	denotes	/
T6666	26971-26972	CD	denotes	5
T6667	26972-26973	HYPH	denotes	/
T6668	26973-26979	NN	denotes	Hdhex4
T6669	26979-26980	HYPH	denotes	/
T6670	26980-26981	CD	denotes	5
T6664	26982-26989	NNS	denotes	embryos
T6671	26990-26992	VBZ	denotes	is
T6661	26993-27003	VBN	denotes	associated
T6672	27004-27008	IN	denotes	with
T6673	27009-27015	RB	denotes	mildly
T6674	27016-27024	VBN	denotes	elevated
T6676	27025-27031	NNS	denotes	levels
T6675	27032-27042	NN	denotes	expression
T6677	27043-27045	IN	denotes	of
T6678	27046-27050	NN	denotes	Hnf4
T6679	27051-27053	IN	denotes	in
T6680	27054-27057	DT	denotes	the
T6682	27058-27067	JJ	denotes	primitive
T6681	27068-27076	NN	denotes	endoderm
T6683	27077-27080	CC	denotes	and
T6684	27081-27084	NN	denotes	Pem
T6685	27085-27087	IN	denotes	in
T6686	27088-27091	DT	denotes	the
T6688	27092-27100	JJ	denotes	lopsided
T6689	27101-27109	JJ	denotes	anterior
T6687	27110-27117	NN	denotes	chorion
T6690	27118-27121	CC	denotes	but
T6691	27122-27125	DT	denotes	the
T6692	27126-27136	NN	denotes	expression
T6694	27137-27139	IN	denotes	of
T6695	27140-27145	JJ	denotes	other
T6697	27146-27151	JJ	denotes	known
T6696	27152-27159	NNS	denotes	signals
T6698	27159-27161	,	denotes	,
T6699	27161-27165	JJ	denotes	such
T6700	27166-27168	IN	denotes	as
T6701	27169-27173	NN	denotes	Bmp4
T6702	27174-27178	IN	denotes	from
T6703	27179-27182	DT	denotes	the
T6705	27183-27197	JJ	denotes	extraembryonic
T6704	27198-27206	NN	denotes	ectoderm
T6706	27206-27208	,	denotes	,
T6707	27208-27211	CC	denotes	and
T6708	27212-27216	NN	denotes	Dkk1
T6709	27217-27220	CC	denotes	and
T6710	27221-27227	NN	denotes	Lefty1
T6711	27228-27232	IN	denotes	from
T6712	27233-27236	DT	denotes	the
T6713	27237-27240	NN	denotes	AVE
T6714	27240-27242	,	denotes	,
T6693	27242-27248	VBP	denotes	appear
T6715	27249-27251	TO	denotes	to
T6716	27252-27254	VB	denotes	be
T6717	27255-27261	JJ	denotes	normal
T6718	27261-27263	,	denotes	,
T6719	27263-27271	IN	denotes	although
T6721	27272-27275	DT	denotes	the
T6723	27276-27282	JJ	denotes	slight
T6722	27283-27291	NN	denotes	increase
T6724	27292-27294	IN	denotes	in
T6725	27295-27298	NN	denotes	Dkk
T6727	27298-27299	HYPH	denotes	-
T6728	27299-27300	CD	denotes	1
T6726	27301-27311	NN	denotes	expression
T6729	27312-27314	IN	denotes	in
T6730	27315-27321	NN	denotes	Hdhex4
T6732	27321-27322	HYPH	denotes	/
T6733	27322-27323	CD	denotes	5
T6734	27323-27324	HYPH	denotes	/
T6735	27324-27330	NN	denotes	Hdhex4
T6736	27330-27331	HYPH	denotes	/
T6737	27331-27332	CD	denotes	5
T6731	27333-27340	NNS	denotes	embryos
T6720	27341-27349	VBZ	denotes	suggests
T6738	27350-27354	IN	denotes	that
T6740	27355-27362	JJ	denotes	further
T6741	27363-27376	NN	denotes	investigation
T6742	27377-27381	IN	denotes	into
T6743	27382-27385	NN	denotes	Wnt
T6744	27386-27395	NN	denotes	signaling
T6739	27396-27398	VBZ	denotes	is
T6745	27399-27408	JJ	denotes	warranted
T6746	27408-27409	.	denotes	.
T6747	27409-27632	sentence	denotes	Moreover, extraembryonic Bmp4 signaling is not impaired in the absence of huntingtin, as the induction of PCGs in mutant embryos is normal, implying proper transport and secretion of the appropriate extraembryonic signals.
T6748	27410-27418	RB	denotes	Moreover
T6750	27418-27420	,	denotes	,
T6751	27420-27434	JJ	denotes	extraembryonic
T6753	27435-27439	NN	denotes	Bmp4
T6752	27440-27449	NN	denotes	signaling
T6754	27450-27452	VBZ	denotes	is
T6755	27453-27456	RB	denotes	not
T6749	27457-27465	VBN	denotes	impaired
T6756	27466-27468	IN	denotes	in
T6757	27469-27472	DT	denotes	the
T6758	27473-27480	NN	denotes	absence
T6759	27481-27483	IN	denotes	of
T6760	27484-27494	NN	denotes	huntingtin
T6761	27494-27496	,	denotes	,
T6762	27496-27498	IN	denotes	as
T6764	27499-27502	DT	denotes	the
T6765	27503-27512	NN	denotes	induction
T6766	27513-27515	IN	denotes	of
T6767	27516-27520	NNS	denotes	PCGs
T6768	27521-27523	IN	denotes	in
T6769	27524-27530	NN	denotes	mutant
T6770	27531-27538	NNS	denotes	embryos
T6763	27539-27541	VBZ	denotes	is
T6771	27542-27548	JJ	denotes	normal
T6772	27548-27550	,	denotes	,
T6773	27550-27558	VBG	denotes	implying
T6774	27559-27565	JJ	denotes	proper
T6775	27566-27575	NN	denotes	transport
T6776	27576-27579	CC	denotes	and
T6777	27580-27589	NN	denotes	secretion
T6778	27590-27592	IN	denotes	of
T6779	27593-27596	DT	denotes	the
T6781	27597-27608	JJ	denotes	appropriate
T6782	27609-27623	JJ	denotes	extraembryonic
T6780	27624-27631	NNS	denotes	signals
T6783	27631-27632	.	denotes	.
T6784	27632-27742	sentence	denotes	However, Nodal, Fgf8 and Gsc are expressed ectopically in the visceral endoderm of Hdhex4/5/Hdhex4/5 embryos.
T6785	27633-27640	RB	denotes	However
T6787	27640-27642	,	denotes	,
T6788	27642-27647	NN	denotes	Nodal
T6789	27647-27649	,	denotes	,
T6790	27649-27653	NN	denotes	Fgf8
T6791	27654-27657	CC	denotes	and
T6792	27658-27661	NN	denotes	Gsc
T6793	27662-27665	VBP	denotes	are
T6786	27666-27675	VBN	denotes	expressed
T6794	27676-27687	RB	denotes	ectopically
T6795	27688-27690	IN	denotes	in
T6796	27691-27694	DT	denotes	the
T6798	27695-27703	JJ	denotes	visceral
T6797	27704-27712	NN	denotes	endoderm
T6799	27713-27715	IN	denotes	of
T6800	27716-27722	NN	denotes	Hdhex4
T6802	27722-27723	HYPH	denotes	/
T6803	27723-27724	CD	denotes	5
T6804	27724-27725	HYPH	denotes	/
T6805	27725-27731	NN	denotes	Hdhex4
T6806	27731-27732	HYPH	denotes	/
T6807	27732-27733	CD	denotes	5
T6801	27734-27741	NNS	denotes	embryos
T6808	27741-27742	.	denotes	.
T6809	27742-27880	sentence	denotes	Both Nodal and Fgf8, important growth factors required for normal development of the epiblast, are tightly regulated during gastrulation.
T6810	27743-27747	CC	denotes	Both
T6811	27748-27753	NN	denotes	Nodal
T6813	27754-27757	CC	denotes	and
T6814	27758-27762	NN	denotes	Fgf8
T6815	27762-27764	,	denotes	,
T6816	27764-27773	JJ	denotes	important
T6818	27774-27780	NN	denotes	growth
T6817	27781-27788	NNS	denotes	factors
T6819	27789-27797	VBN	denotes	required
T6820	27798-27801	IN	denotes	for
T6821	27802-27808	JJ	denotes	normal
T6822	27809-27820	NN	denotes	development
T6823	27821-27823	IN	denotes	of
T6824	27824-27827	DT	denotes	the
T6825	27828-27836	NN	denotes	epiblast
T6826	27836-27838	,	denotes	,
T6827	27838-27841	VBP	denotes	are
T6828	27842-27849	RB	denotes	tightly
T6812	27850-27859	VBN	denotes	regulated
T6829	27860-27866	IN	denotes	during
T6830	27867-27879	NN	denotes	gastrulation
T6831	27879-27880	.	denotes	.
T6832	27880-28043	sentence	denotes	Therefore, misexpression of either or both of these factors, or of goosecoid, in the visceral endoderm could contribute to the Hdhex4/5/Hdhex4/5 mutant phenotype.
T6833	27881-27890	RB	denotes	Therefore
T6835	27890-27892	,	denotes	,
T6836	27892-27905	NN	denotes	misexpression
T6837	27906-27908	IN	denotes	of
T6838	27909-27915	DT	denotes	either
T6839	27916-27918	CC	denotes	or
T6840	27919-27923	DT	denotes	both
T6841	27924-27926	IN	denotes	of
T6842	27927-27932	DT	denotes	these
T6843	27933-27940	NNS	denotes	factors
T6844	27940-27942	,	denotes	,
T6845	27942-27944	CC	denotes	or
T6846	27945-27947	IN	denotes	of
T6847	27948-27957	NN	denotes	goosecoid
T6848	27957-27959	,	denotes	,
T6849	27959-27961	IN	denotes	in
T6850	27962-27965	DT	denotes	the
T6852	27966-27974	JJ	denotes	visceral
T6851	27975-27983	NN	denotes	endoderm
T6853	27984-27989	MD	denotes	could
T6834	27990-28000	VB	denotes	contribute
T6854	28001-28003	IN	denotes	to
T6855	28004-28007	DT	denotes	the
T6857	28008-28014	NN	denotes	Hdhex4
T6858	28014-28015	HYPH	denotes	/
T6859	28015-28016	CD	denotes	5
T6860	28016-28017	HYPH	denotes	/
T6861	28017-28023	NN	denotes	Hdhex4
T6862	28023-28024	HYPH	denotes	/
T6863	28024-28025	CD	denotes	5
T6864	28026-28032	NN	denotes	mutant
T6856	28033-28042	NN	denotes	phenotype
T6865	28042-28043	.	denotes	.
T6866	28043-28232	sentence	denotes	In addition, it is possible that other extraembryonic signal(s) that we have not analyzed may also be affected by the lack of huntingtin activity in extraembryonic cells in mutant embryos.
T6867	28044-28046	IN	denotes	In
T6869	28047-28055	NN	denotes	addition
T6870	28055-28057	,	denotes	,
T6871	28057-28059	PRP	denotes	it
T6868	28060-28062	VBZ	denotes	is
T6872	28063-28071	JJ	denotes	possible
T6873	28072-28076	IN	denotes	that
T6875	28077-28082	JJ	denotes	other
T6877	28083-28097	JJ	denotes	extraembryonic
T6876	28098-28104	NN	denotes	signal
T6878	28104-28105	-LRB-	denotes	(
T6879	28105-28106	AFX	denotes	s
T6880	28106-28107	-RRB-	denotes	)
T6881	28108-28112	WDT	denotes	that
T6883	28113-28115	PRP	denotes	we
T6884	28116-28120	VBP	denotes	have
T6885	28121-28124	RB	denotes	not
T6882	28125-28133	VBN	denotes	analyzed
T6886	28134-28137	MD	denotes	may
T6887	28138-28142	RB	denotes	also
T6888	28143-28145	VB	denotes	be
T6874	28146-28154	VBN	denotes	affected
T6889	28155-28157	IN	denotes	by
T6890	28158-28161	DT	denotes	the
T6891	28162-28166	NN	denotes	lack
T6892	28167-28169	IN	denotes	of
T6893	28170-28180	NN	denotes	huntingtin
T6894	28181-28189	NN	denotes	activity
T6895	28190-28192	IN	denotes	in
T6896	28193-28207	JJ	denotes	extraembryonic
T6897	28208-28213	NNS	denotes	cells
T6898	28214-28216	IN	denotes	in
T6899	28217-28223	NN	denotes	mutant
T6900	28224-28231	NNS	denotes	embryos
T6901	28231-28232	.	denotes	.
T6902	28232-28365	sentence	denotes	Huntingtin deficient embryos also fail to form headfolds, and to undergo organogenesis, even after culturing in nutrient rich media.
T6903	28233-28243	NN	denotes	Huntingtin
T6904	28244-28253	JJ	denotes	deficient
T6905	28254-28261	NNS	denotes	embryos
T6907	28262-28266	RB	denotes	also
T6906	28267-28271	VBP	denotes	fail
T6908	28272-28274	TO	denotes	to
T6909	28275-28279	VB	denotes	form
T6910	28280-28289	NNS	denotes	headfolds
T6911	28289-28291	,	denotes	,
T6912	28291-28294	CC	denotes	and
T6913	28295-28297	TO	denotes	to
T6914	28298-28305	VB	denotes	undergo
T6915	28306-28319	NN	denotes	organogenesis
T6916	28319-28321	,	denotes	,
T6917	28321-28325	RB	denotes	even
T6918	28326-28331	IN	denotes	after
T6919	28332-28341	VBG	denotes	culturing
T6920	28342-28344	IN	denotes	in
T6921	28345-28353	NN	denotes	nutrient
T6922	28354-28358	JJ	denotes	rich
T6923	28359-28364	NNS	denotes	media
T6924	28364-28365	.	denotes	.
T6925	28365-28588	sentence	denotes	The absence of headfold formation in these embryos does not appear to be a result of a failure to induce neurectoderm or a failure to form the AVE, since mutant embryos express markers such as Otx2, Ddk1, Lefty1 and Hesx1.
T6926	28366-28369	DT	denotes	The
T6927	28370-28377	NN	denotes	absence
T6929	28378-28380	IN	denotes	of
T6930	28381-28389	NN	denotes	headfold
T6931	28390-28399	NN	denotes	formation
T6932	28400-28402	IN	denotes	in
T6933	28403-28408	DT	denotes	these
T6934	28409-28416	NNS	denotes	embryos
T6935	28417-28421	VBZ	denotes	does
T6936	28422-28425	RB	denotes	not
T6928	28426-28432	VB	denotes	appear
T6937	28433-28435	TO	denotes	to
T6938	28436-28438	VB	denotes	be
T6939	28439-28440	DT	denotes	a
T6940	28441-28447	NN	denotes	result
T6941	28448-28450	IN	denotes	of
T6942	28451-28452	DT	denotes	a
T6943	28453-28460	NN	denotes	failure
T6944	28461-28463	TO	denotes	to
T6945	28464-28470	VB	denotes	induce
T6946	28471-28483	NN	denotes	neurectoderm
T6947	28484-28486	CC	denotes	or
T6948	28487-28488	DT	denotes	a
T6949	28489-28496	NN	denotes	failure
T6950	28497-28499	TO	denotes	to
T6951	28500-28504	VB	denotes	form
T6952	28505-28508	DT	denotes	the
T6953	28509-28512	NN	denotes	AVE
T6954	28512-28514	,	denotes	,
T6955	28514-28519	IN	denotes	since
T6957	28520-28526	NN	denotes	mutant
T6958	28527-28534	NNS	denotes	embryos
T6956	28535-28542	VBP	denotes	express
T6959	28543-28550	NNS	denotes	markers
T6960	28551-28555	JJ	denotes	such
T6961	28556-28558	IN	denotes	as
T6962	28559-28563	NN	denotes	Otx2
T6963	28563-28565	,	denotes	,
T6964	28565-28569	NN	denotes	Ddk1
T6965	28569-28571	,	denotes	,
T6966	28571-28577	NN	denotes	Lefty1
T6967	28578-28581	CC	denotes	and
T6968	28582-28587	NN	denotes	Hesx1
T6969	28587-28588	.	denotes	.
T6970	28588-28778	sentence	denotes	In addition, since node formation is not required for neural induction [44-46], the failure to form a node in huntingtin deficient embryos is also unlikely to explain the lack of headfolds.
T6971	28589-28591	IN	denotes	In
T6973	28592-28600	NN	denotes	addition
T6974	28600-28602	,	denotes	,
T6975	28602-28607	IN	denotes	since
T6977	28608-28612	NN	denotes	node
T6978	28613-28622	NN	denotes	formation
T6979	28623-28625	VBZ	denotes	is
T6980	28626-28629	RB	denotes	not
T6976	28630-28638	VBN	denotes	required
T6981	28639-28642	IN	denotes	for
T6982	28643-28649	JJ	denotes	neural
T6983	28650-28659	NN	denotes	induction
T6984	28660-28661	-LRB-	denotes	[
T6985	28661-28663	CD	denotes	44
T6986	28663-28664	SYM	denotes	-
T6987	28664-28666	CD	denotes	46
T6988	28666-28667	-RRB-	denotes	]
T6989	28667-28669	,	denotes	,
T6990	28669-28672	DT	denotes	the
T6991	28673-28680	NN	denotes	failure
T6992	28681-28683	TO	denotes	to
T6993	28684-28688	VB	denotes	form
T6994	28689-28690	DT	denotes	a
T6995	28691-28695	NN	denotes	node
T6996	28696-28698	IN	denotes	in
T6997	28699-28709	NN	denotes	huntingtin
T6998	28710-28719	JJ	denotes	deficient
T6999	28720-28727	NNS	denotes	embryos
T6972	28728-28730	VBZ	denotes	is
T7000	28731-28735	RB	denotes	also
T7001	28736-28744	JJ	denotes	unlikely
T7002	28745-28747	TO	denotes	to
T7003	28748-28755	VB	denotes	explain
T7004	28756-28759	DT	denotes	the
T7005	28760-28764	NN	denotes	lack
T7006	28765-28767	IN	denotes	of
T7007	28768-28777	NNS	denotes	headfolds
T7008	28777-28778	.	denotes	.
T7009	28778-28996	sentence	denotes	The apparent reduction of paraxial mesoderm in Hdhex4/5/Hdhex4/5 embryos could explain the lack of headfolds since paraxial mesoderm is important for the full development of neuroectoderm, and consequently, headfolds.
T7010	28779-28782	DT	denotes	The
T7012	28783-28791	JJ	denotes	apparent
T7011	28792-28801	NN	denotes	reduction
T7014	28802-28804	IN	denotes	of
T7015	28805-28813	JJ	denotes	paraxial
T7016	28814-28822	NN	denotes	mesoderm
T7017	28823-28825	IN	denotes	in
T7018	28826-28832	NN	denotes	Hdhex4
T7020	28832-28833	HYPH	denotes	/
T7021	28833-28834	CD	denotes	5
T7022	28834-28835	HYPH	denotes	/
T7023	28835-28841	NN	denotes	Hdhex4
T7024	28841-28842	HYPH	denotes	/
T7025	28842-28843	CD	denotes	5
T7019	28844-28851	NNS	denotes	embryos
T7026	28852-28857	MD	denotes	could
T7013	28858-28865	VB	denotes	explain
T7027	28866-28869	DT	denotes	the
T7028	28870-28874	NN	denotes	lack
T7029	28875-28877	IN	denotes	of
T7030	28878-28887	NNS	denotes	headfolds
T7031	28888-28893	IN	denotes	since
T7033	28894-28902	JJ	denotes	paraxial
T7034	28903-28911	NN	denotes	mesoderm
T7032	28912-28914	VBZ	denotes	is
T7035	28915-28924	JJ	denotes	important
T7036	28925-28928	IN	denotes	for
T7037	28929-28932	DT	denotes	the
T7039	28933-28937	JJ	denotes	full
T7038	28938-28949	NN	denotes	development
T7040	28950-28952	IN	denotes	of
T7041	28953-28966	NN	denotes	neuroectoderm
T7042	28966-28968	,	denotes	,
T7043	28968-28971	CC	denotes	and
T7044	28972-28984	RB	denotes	consequently
T7046	28984-28986	,	denotes	,
T7045	28986-28995	NNS	denotes	headfolds
T7047	28995-28996	.	denotes	.
T7048	28996-29139	sentence	denotes	Alternatively, the inability to manifest headfolds could suggest that huntingtin is required at a very early stage for normal CNS development.
T7049	28997-29010	RB	denotes	Alternatively
T7051	29010-29012	,	denotes	,
T7052	29012-29015	DT	denotes	the
T7053	29016-29025	NN	denotes	inability
T7054	29026-29028	TO	denotes	to
T7055	29029-29037	VB	denotes	manifest
T7056	29038-29047	NNS	denotes	headfolds
T7057	29048-29053	MD	denotes	could
T7050	29054-29061	VB	denotes	suggest
T7058	29062-29066	IN	denotes	that
T7060	29067-29077	NN	denotes	huntingtin
T7061	29078-29080	VBZ	denotes	is
T7059	29081-29089	VBN	denotes	required
T7062	29090-29092	IN	denotes	at
T7063	29093-29094	DT	denotes	a
T7065	29095-29099	RB	denotes	very
T7066	29100-29105	JJ	denotes	early
T7064	29106-29111	NN	denotes	stage
T7067	29112-29115	IN	denotes	for
T7068	29116-29122	JJ	denotes	normal
T7070	29123-29126	NN	denotes	CNS
T7069	29127-29138	NN	denotes	development
T7071	29138-29139	.	denotes	.
T7072	29139-29322	sentence	denotes	This conclusion is consistent with the finding that severely reduced levels of huntingtin, from a hypomorphic Hdh allele, lead to abnormal brains later in embryonic development [15].
T7073	29140-29144	DT	denotes	This
T7074	29145-29155	NN	denotes	conclusion
T7075	29156-29158	VBZ	denotes	is
T7076	29159-29169	JJ	denotes	consistent
T7077	29170-29174	IN	denotes	with
T7078	29175-29178	DT	denotes	the
T7079	29179-29186	NN	denotes	finding
T7080	29187-29191	IN	denotes	that
T7082	29192-29200	RB	denotes	severely
T7083	29201-29208	VBN	denotes	reduced
T7084	29209-29215	NNS	denotes	levels
T7085	29216-29218	IN	denotes	of
T7086	29219-29229	NN	denotes	huntingtin
T7087	29229-29231	,	denotes	,
T7088	29231-29235	IN	denotes	from
T7089	29236-29237	DT	denotes	a
T7091	29238-29249	JJ	denotes	hypomorphic
T7092	29250-29253	NN	denotes	Hdh
T7090	29254-29260	NN	denotes	allele
T7093	29260-29262	,	denotes	,
T7081	29262-29266	VBP	denotes	lead
T7094	29267-29269	IN	denotes	to
T7095	29270-29278	JJ	denotes	abnormal
T7096	29279-29285	NNS	denotes	brains
T7097	29286-29291	RBR	denotes	later
T7098	29292-29294	IN	denotes	in
T7099	29295-29304	JJ	denotes	embryonic
T7100	29305-29316	NN	denotes	development
T7101	29317-29318	-LRB-	denotes	[
T7102	29318-29320	CD	denotes	15
T7103	29320-29321	-RRB-	denotes	]
T7104	29321-29322	.	denotes	.
T7105	29322-29532	sentence	denotes	The cardinal features of complete Hdh inactivation that we observe are similar to the phenotypes that stem from the complete inactivation of the Polycomb group gene (Pc-g) Eed (embryonic ectoderm development).
T7106	29323-29326	DT	denotes	The
T7108	29327-29335	JJ	denotes	cardinal
T7107	29336-29344	NNS	denotes	features
T7110	29345-29347	IN	denotes	of
T7111	29348-29356	JJ	denotes	complete
T7113	29357-29360	NN	denotes	Hdh
T7112	29361-29373	NN	denotes	inactivation
T7114	29374-29378	WDT	denotes	that
T7116	29379-29381	PRP	denotes	we
T7115	29382-29389	VBP	denotes	observe
T7109	29390-29393	VBP	denotes	are
T7117	29394-29401	JJ	denotes	similar
T7118	29402-29404	IN	denotes	to
T7119	29405-29408	DT	denotes	the
T7120	29409-29419	NNS	denotes	phenotypes
T7121	29420-29424	WDT	denotes	that
T7122	29425-29429	VBP	denotes	stem
T7123	29430-29434	IN	denotes	from
T7124	29435-29438	DT	denotes	the
T7126	29439-29447	JJ	denotes	complete
T7125	29448-29460	NN	denotes	inactivation
T7127	29461-29463	IN	denotes	of
T7128	29464-29467	DT	denotes	the
T7130	29468-29476	NN	denotes	Polycomb
T7131	29477-29482	NN	denotes	group
T7129	29483-29487	NN	denotes	gene
T7132	29488-29489	-LRB-	denotes	(
T7133	29489-29491	NN	denotes	Pc
T7135	29491-29492	HYPH	denotes	-
T7134	29492-29493	NN	denotes	g
T7136	29493-29494	-RRB-	denotes	)
T7137	29495-29498	NN	denotes	Eed
T7138	29499-29500	-LRB-	denotes	(
T7139	29500-29509	JJ	denotes	embryonic
T7141	29510-29518	NN	denotes	ectoderm
T7140	29519-29530	NN	denotes	development
T7142	29530-29531	-RRB-	denotes	)
T7143	29531-29532	.	denotes	.
T7144	29532-29774	sentence	denotes	Indeed, complete deficiency for either huntingtin or the eed protein leads to abnormal streak development, lack of headfold formation, ectopic T, Evx1 and Nodal expression and disruption of anterior primitive streak mesoderm production [47].
T7145	29533-29539	RB	denotes	Indeed
T7147	29539-29541	,	denotes	,
T7148	29541-29549	JJ	denotes	complete
T7149	29550-29560	NN	denotes	deficiency
T7150	29561-29564	IN	denotes	for
T7151	29565-29571	CC	denotes	either
T7152	29572-29582	NN	denotes	huntingtin
T7153	29583-29585	CC	denotes	or
T7154	29586-29589	DT	denotes	the
T7156	29590-29593	NN	denotes	eed
T7155	29594-29601	NN	denotes	protein
T7146	29602-29607	VBZ	denotes	leads
T7157	29608-29610	IN	denotes	to
T7158	29611-29619	JJ	denotes	abnormal
T7160	29620-29626	NN	denotes	streak
T7159	29627-29638	NN	denotes	development
T7161	29638-29640	,	denotes	,
T7162	29640-29644	NN	denotes	lack
T7163	29645-29647	IN	denotes	of
T7164	29648-29656	NN	denotes	headfold
T7165	29657-29666	NN	denotes	formation
T7166	29666-29668	,	denotes	,
T7167	29668-29675	JJ	denotes	ectopic
T7168	29676-29677	NN	denotes	T
T7169	29677-29679	,	denotes	,
T7170	29679-29683	NN	denotes	Evx1
T7171	29684-29687	CC	denotes	and
T7172	29688-29693	NN	denotes	Nodal
T7173	29694-29704	NN	denotes	expression
T7174	29705-29708	CC	denotes	and
T7175	29709-29719	NN	denotes	disruption
T7176	29720-29722	IN	denotes	of
T7177	29723-29731	JJ	denotes	anterior
T7179	29732-29741	JJ	denotes	primitive
T7180	29742-29748	NN	denotes	streak
T7178	29749-29757	NN	denotes	mesoderm
T7181	29758-29768	NN	denotes	production
T7182	29769-29770	-LRB-	denotes	[
T7183	29770-29772	CD	denotes	47
T7184	29772-29773	-RRB-	denotes	]
T7185	29773-29774	.	denotes	.
T7186	29774-30024	sentence	denotes	Interestingly, Eed protein is also required for proper trophoblast development and normal maintenance of imprinted X-inactivation and genomic imprinting [47-49], suggesting that these activities warrant investigation in huntingtin deficient embryos.
T7187	29775-29788	RB	denotes	Interestingly
T7189	29788-29790	,	denotes	,
T7190	29790-29793	NN	denotes	Eed
T7191	29794-29801	NN	denotes	protein
T7192	29802-29804	VBZ	denotes	is
T7193	29805-29809	RB	denotes	also
T7188	29810-29818	VBN	denotes	required
T7194	29819-29822	IN	denotes	for
T7195	29823-29829	JJ	denotes	proper
T7197	29830-29841	NN	denotes	trophoblast
T7196	29842-29853	NN	denotes	development
T7198	29854-29857	CC	denotes	and
T7199	29858-29864	JJ	denotes	normal
T7200	29865-29876	NN	denotes	maintenance
T7201	29877-29879	IN	denotes	of
T7202	29880-29889	VBN	denotes	imprinted
T7204	29890-29891	NN	denotes	X
T7205	29891-29892	HYPH	denotes	-
T7203	29892-29904	NN	denotes	inactivation
T7206	29905-29908	CC	denotes	and
T7207	29909-29916	JJ	denotes	genomic
T7208	29917-29927	NN	denotes	imprinting
T7209	29928-29929	-LRB-	denotes	[
T7210	29929-29931	CD	denotes	47
T7211	29931-29932	SYM	denotes	-
T7212	29932-29934	CD	denotes	49
T7213	29934-29935	-RRB-	denotes	]
T7214	29935-29937	,	denotes	,
T7215	29937-29947	VBG	denotes	suggesting
T7216	29948-29952	IN	denotes	that
T7218	29953-29958	DT	denotes	these
T7219	29959-29969	NNS	denotes	activities
T7217	29970-29977	VBP	denotes	warrant
T7220	29978-29991	NN	denotes	investigation
T7221	29992-29994	IN	denotes	in
T7222	29995-30005	NN	denotes	huntingtin
T7223	30006-30015	JJ	denotes	deficient
T7224	30016-30023	NNS	denotes	embryos
T7225	30023-30024	.	denotes	.
T7226	30024-30296	sentence	denotes	Thus, our observations provide unexpected starting-points in the search for huntingtin's precise molecular activities, which began with the discovery that this HEAT domain protein hosts the dominant polyglutamine property that is the fundamental basis of HD pathogenesis.
T7227	30025-30029	RB	denotes	Thus
T7229	30029-30031	,	denotes	,
T7230	30031-30034	PRP$	denotes	our
T7231	30035-30047	NNS	denotes	observations
T7228	30048-30055	VBP	denotes	provide
T7232	30056-30066	JJ	denotes	unexpected
T7234	30067-30075	VBG	denotes	starting
T7235	30075-30076	HYPH	denotes	-
T7233	30076-30082	NNS	denotes	points
T7236	30083-30085	IN	denotes	in
T7237	30086-30089	DT	denotes	the
T7238	30090-30096	NN	denotes	search
T7239	30097-30100	IN	denotes	for
T7240	30101-30111	NN	denotes	huntingtin
T7242	30111-30113	POS	denotes	's
T7243	30114-30121	JJ	denotes	precise
T7244	30122-30131	JJ	denotes	molecular
T7241	30132-30142	NNS	denotes	activities
T7245	30142-30144	,	denotes	,
T7246	30144-30149	WDT	denotes	which
T7247	30150-30155	VBD	denotes	began
T7248	30156-30160	IN	denotes	with
T7249	30161-30164	DT	denotes	the
T7250	30165-30174	NN	denotes	discovery
T7251	30175-30179	IN	denotes	that
T7253	30180-30184	DT	denotes	this
T7255	30185-30189	NN	denotes	HEAT
T7256	30190-30196	NN	denotes	domain
T7254	30197-30204	NN	denotes	protein
T7252	30205-30210	VBZ	denotes	hosts
T7257	30211-30214	DT	denotes	the
T7259	30215-30223	JJ	denotes	dominant
T7260	30224-30237	NN	denotes	polyglutamine
T7258	30238-30246	NN	denotes	property
T7261	30247-30251	WDT	denotes	that
T7262	30252-30254	VBZ	denotes	is
T7263	30255-30258	DT	denotes	the
T7265	30259-30270	JJ	denotes	fundamental
T7264	30271-30276	NN	denotes	basis
T7266	30277-30279	IN	denotes	of
T7267	30280-30282	NN	denotes	HD
T7268	30283-30295	NN	denotes	pathogenesis
T7269	30295-30296	.	denotes	.
T7270	30296-30545	sentence	denotes	In HD patients and in accurate genetic replicas, HD CAG knock-in mice, the dominant mutation specifically affects the major population of neurons in the striatum, without impairing huntingtin's essential activities in embryonic development [50-53].
T7271	30297-30299	IN	denotes	In
T7273	30300-30302	NN	denotes	HD
T7274	30303-30311	NNS	denotes	patients
T7275	30312-30315	CC	denotes	and
T7276	30316-30318	IN	denotes	in
T7277	30319-30327	JJ	denotes	accurate
T7279	30328-30335	JJ	denotes	genetic
T7278	30336-30344	NNS	denotes	replicas
T7280	30344-30346	,	denotes	,
T7281	30346-30348	NN	denotes	HD
T7283	30349-30352	NN	denotes	CAG
T7284	30353-30358	VB	denotes	knock
T7285	30358-30359	HYPH	denotes	-
T7286	30359-30361	RP	denotes	in
T7282	30362-30366	NNS	denotes	mice
T7287	30366-30368	,	denotes	,
T7288	30368-30371	DT	denotes	the
T7290	30372-30380	JJ	denotes	dominant
T7289	30381-30389	NN	denotes	mutation
T7291	30390-30402	RB	denotes	specifically
T7272	30403-30410	VBZ	denotes	affects
T7292	30411-30414	DT	denotes	the
T7294	30415-30420	JJ	denotes	major
T7293	30421-30431	NN	denotes	population
T7295	30432-30434	IN	denotes	of
T7296	30435-30442	NNS	denotes	neurons
T7297	30443-30445	IN	denotes	in
T7298	30446-30449	DT	denotes	the
T7299	30450-30458	NN	denotes	striatum
T7300	30458-30460	,	denotes	,
T7301	30460-30467	IN	denotes	without
T7302	30468-30477	VBG	denotes	impairing
T7303	30478-30488	NN	denotes	huntingtin
T7305	30488-30490	POS	denotes	's
T7306	30491-30500	JJ	denotes	essential
T7304	30501-30511	NNS	denotes	activities
T7307	30512-30514	IN	denotes	in
T7308	30515-30524	JJ	denotes	embryonic
T7309	30525-30536	NN	denotes	development
T7310	30537-30538	-LRB-	denotes	[
T7311	30538-30540	CD	denotes	50
T7312	30540-30541	SYM	denotes	-
T7313	30541-30543	CD	denotes	53
T7314	30543-30544	-RRB-	denotes	]
T7315	30544-30545	.	denotes	.
T7316	30545-30750	sentence	denotes	Indeed, homozygous HD patients make no wild-type huntingtin, and, in the mouse, a single mutant Hdh allele's worth of mutant huntingtin can fully rescue huntingtin deficiency embryonic phenotypes [15,51].
T7317	30546-30552	RB	denotes	Indeed
T7319	30552-30554	,	denotes	,
T7320	30554-30564	JJ	denotes	homozygous
T7322	30565-30567	NN	denotes	HD
T7321	30568-30576	NNS	denotes	patients
T7318	30577-30581	VBP	denotes	make
T7323	30582-30584	DT	denotes	no
T7325	30585-30589	JJ	denotes	wild
T7327	30589-30590	HYPH	denotes	-
T7326	30590-30594	NN	denotes	type
T7324	30595-30605	NN	denotes	huntingtin
T7328	30605-30607	,	denotes	,
T7329	30607-30610	CC	denotes	and
T7330	30610-30612	,	denotes	,
T7331	30612-30614	IN	denotes	in
T7333	30615-30618	DT	denotes	the
T7334	30619-30624	NN	denotes	mouse
T7335	30624-30626	,	denotes	,
T7336	30626-30627	DT	denotes	a
T7338	30628-30634	JJ	denotes	single
T7339	30635-30641	NN	denotes	mutant
T7340	30642-30645	NN	denotes	Hdh
T7337	30646-30652	NN	denotes	allele
T7342	30652-30654	POS	denotes	's
T7341	30655-30660	NN	denotes	worth
T7343	30661-30663	IN	denotes	of
T7344	30664-30670	NN	denotes	mutant
T7345	30671-30681	NN	denotes	huntingtin
T7346	30682-30685	MD	denotes	can
T7347	30686-30691	RB	denotes	fully
T7332	30692-30698	VB	denotes	rescue
T7348	30699-30709	NN	denotes	huntingtin
T7349	30710-30720	NN	denotes	deficiency
T7351	30721-30730	JJ	denotes	embryonic
T7350	30731-30741	NNS	denotes	phenotypes
T7352	30742-30743	-LRB-	denotes	[
T7354	30743-30745	CD	denotes	15
T7355	30745-30746	,	denotes	,
T7353	30746-30748	CD	denotes	51
T7356	30748-30749	-RRB-	denotes	]
T7357	30749-30750	.	denotes	.
T7358	30750-30949	sentence	denotes	The quest to understand the HD mechanism, therefore, is aimed at delineating the huntingtin activity that may explain the striatal cell specificity of the polyglutamine mutant version of huntingtin.
T7359	30751-30754	DT	denotes	The
T7360	30755-30760	NN	denotes	quest
T7362	30761-30763	TO	denotes	to
T7363	30764-30774	VB	denotes	understand
T7364	30775-30778	DT	denotes	the
T7366	30779-30781	NN	denotes	HD
T7365	30782-30791	NN	denotes	mechanism
T7367	30791-30793	,	denotes	,
T7368	30793-30802	RB	denotes	therefore
T7369	30802-30804	,	denotes	,
T7370	30804-30806	VBZ	denotes	is
T7361	30807-30812	VBN	denotes	aimed
T7371	30813-30815	IN	denotes	at
T7372	30816-30827	VBG	denotes	delineating
T7373	30828-30831	DT	denotes	the
T7375	30832-30842	NN	denotes	huntingtin
T7374	30843-30851	NN	denotes	activity
T7376	30852-30856	WDT	denotes	that
T7378	30857-30860	MD	denotes	may
T7377	30861-30868	VB	denotes	explain
T7379	30869-30872	DT	denotes	the
T7381	30873-30881	JJ	denotes	striatal
T7382	30882-30886	NN	denotes	cell
T7380	30887-30898	NN	denotes	specificity
T7383	30899-30901	IN	denotes	of
T7384	30902-30905	DT	denotes	the
T7386	30906-30919	NN	denotes	polyglutamine
T7387	30920-30926	NN	denotes	mutant
T7385	30927-30934	NN	denotes	version
T7388	30935-30937	IN	denotes	of
T7389	30938-30948	NN	denotes	huntingtin
T7390	30948-30949	.	denotes	.
T7391	30949-31084	sentence	denotes	One hypothesis is that huntingtin is normally involved in gene transcription, as proposed for NRSF/REST mediated BDNF expression [54].
T7392	30950-30953	CD	denotes	One
T7393	30954-30964	NN	denotes	hypothesis
T7394	30965-30967	VBZ	denotes	is
T7395	30968-30972	IN	denotes	that
T7397	30973-30983	NN	denotes	huntingtin
T7398	30984-30986	VBZ	denotes	is
T7399	30987-30995	RB	denotes	normally
T7396	30996-31004	VBN	denotes	involved
T7400	31005-31007	IN	denotes	in
T7401	31008-31012	NN	denotes	gene
T7402	31013-31026	NN	denotes	transcription
T7403	31026-31028	,	denotes	,
T7404	31028-31030	IN	denotes	as
T7405	31031-31039	VBN	denotes	proposed
T7406	31040-31043	IN	denotes	for
T7407	31044-31048	NN	denotes	NRSF
T7409	31048-31049	HYPH	denotes	/
T7408	31049-31053	NN	denotes	REST
T7410	31054-31062	VBN	denotes	mediated
T7412	31063-31067	NN	denotes	BDNF
T7411	31068-31078	NN	denotes	expression
T7413	31079-31080	-LRB-	denotes	[
T7414	31080-31082	CD	denotes	54
T7415	31082-31083	-RRB-	denotes	]
T7416	31083-31084	.	denotes	.
T7417	31084-31351	sentence	denotes	Now, our finding that huntingtin can be absolutely necessary for the appropriate regulation of genes with dynamic expression patterns in vivo, provides a compelling reason to elucidate the cellular machinery that is necessary for huntingtin mediated gene regulation.
T7418	31085-31088	RB	denotes	Now
T7420	31088-31090	,	denotes	,
T7421	31090-31093	PRP$	denotes	our
T7422	31094-31101	NN	denotes	finding
T7423	31102-31106	IN	denotes	that
T7425	31107-31117	NN	denotes	huntingtin
T7426	31118-31121	MD	denotes	can
T7424	31122-31124	VB	denotes	be
T7427	31125-31135	RB	denotes	absolutely
T7428	31136-31145	JJ	denotes	necessary
T7429	31146-31149	IN	denotes	for
T7430	31150-31153	DT	denotes	the
T7432	31154-31165	JJ	denotes	appropriate
T7431	31166-31176	NN	denotes	regulation
T7433	31177-31179	IN	denotes	of
T7434	31180-31185	NNS	denotes	genes
T7435	31186-31190	IN	denotes	with
T7436	31191-31198	JJ	denotes	dynamic
T7438	31199-31209	NN	denotes	expression
T7437	31210-31218	NNS	denotes	patterns
T7439	31219-31221	FW	denotes	in
T7440	31222-31226	FW	denotes	vivo
T7441	31226-31228	,	denotes	,
T7419	31228-31236	VBZ	denotes	provides
T7442	31237-31238	DT	denotes	a
T7444	31239-31249	JJ	denotes	compelling
T7443	31250-31256	NN	denotes	reason
T7445	31257-31259	TO	denotes	to
T7446	31260-31269	VB	denotes	elucidate
T7447	31270-31273	DT	denotes	the
T7449	31274-31282	JJ	denotes	cellular
T7448	31283-31292	NN	denotes	machinery
T7450	31293-31297	WDT	denotes	that
T7451	31298-31300	VBZ	denotes	is
T7452	31301-31310	JJ	denotes	necessary
T7453	31311-31314	IN	denotes	for
T7454	31315-31325	NN	denotes	huntingtin
T7455	31326-31334	VBN	denotes	mediated
T7457	31335-31339	NN	denotes	gene
T7456	31340-31350	NN	denotes	regulation
T7458	31350-31351	.	denotes	.
T7532	31364-31367	PRP$	denotes	Our
T7533	31368-31376	NNS	denotes	findings
T7534	31377-31385	VBP	denotes	indicate
T7535	31386-31390	IN	denotes	that
T7537	31391-31401	NN	denotes	huntingtin
T7538	31402-31404	VBZ	denotes	is
T7536	31405-31413	VBN	denotes	required
T7539	31414-31417	IN	denotes	for
T7540	31418-31424	JJ	denotes	proper
T7541	31425-31435	NN	denotes	patterning
T7542	31436-31438	IN	denotes	of
T7543	31439-31442	DT	denotes	the
T7544	31443-31451	NN	denotes	epiblast
T7545	31452-31458	IN	denotes	during
T7546	31459-31464	JJ	denotes	early
T7547	31465-31478	NN	denotes	embryogenesis
T7548	31478-31480	,	denotes	,
T7549	31480-31483	IN	denotes	for
T7550	31484-31490	JJ	denotes	proper
T7552	31491-31499	JJ	denotes	anterior
T7553	31500-31506	NN	denotes	streak
T7554	31507-31510	CC	denotes	and
T7555	31511-31515	NN	denotes	node
T7551	31516-31525	NN	denotes	formation
T7556	31525-31527	,	denotes	,
T7557	31527-31536	JJ	denotes	primitive
T7558	31537-31543	NN	denotes	streak
T7559	31544-31555	NN	denotes	progression
T7560	31555-31557	,	denotes	,
T7561	31557-31565	JJ	denotes	paraxial
T7562	31566-31574	NN	denotes	mesoderm
T7564	31575-31578	CC	denotes	and
T7565	31579-31583	NN	denotes	head
T7566	31584-31588	NN	denotes	fold
T7563	31589-31598	NN	denotes	formation
T7567	31598-31600	,	denotes	,
T7568	31600-31602	RB	denotes	as
T7570	31603-31607	RB	denotes	well
T7569	31608-31610	IN	denotes	as
T7571	31611-31614	IN	denotes	for
T7572	31615-31618	DT	denotes	the
T7574	31619-31625	JJ	denotes	proper
T7573	31626-31637	NN	denotes	restriction
T7575	31638-31640	IN	denotes	of
T7576	31641-31652	RB	denotes	transiently
T7577	31653-31662	VBN	denotes	expressed
T7579	31663-31669	NN	denotes	growth
T7581	31670-31673	CC	denotes	and
T7582	31674-31687	NN	denotes	transcription
T7580	31688-31694	NN	denotes	factor
T7578	31695-31700	NNS	denotes	genes
T7583	31700-31701	.	denotes	.
T7584	31701-31890	sentence	denotes	Knowledge of the molecular basis of these changes in huntingtin deficient embryos should facilitate the identification of the cellular pathways that are dependent on huntingtin activities.
T7585	31702-31711	NN	denotes	Knowledge
T7587	31712-31714	IN	denotes	of
T7588	31715-31718	DT	denotes	the
T7590	31719-31728	JJ	denotes	molecular
T7589	31729-31734	NN	denotes	basis
T7591	31735-31737	IN	denotes	of
T7592	31738-31743	DT	denotes	these
T7593	31744-31751	NNS	denotes	changes
T7594	31752-31754	IN	denotes	in
T7595	31755-31765	NN	denotes	huntingtin
T7596	31766-31775	JJ	denotes	deficient
T7597	31776-31783	NNS	denotes	embryos
T7598	31784-31790	MD	denotes	should
T7586	31791-31801	VB	denotes	facilitate
T7599	31802-31805	DT	denotes	the
T7600	31806-31820	NN	denotes	identification
T7601	31821-31823	IN	denotes	of
T7602	31824-31827	DT	denotes	the
T7604	31828-31836	JJ	denotes	cellular
T7603	31837-31845	NNS	denotes	pathways
T7605	31846-31850	WDT	denotes	that
T7606	31851-31854	VBP	denotes	are
T7607	31855-31864	JJ	denotes	dependent
T7608	31865-31867	IN	denotes	on
T7609	31868-31878	NN	denotes	huntingtin
T7610	31879-31889	NNS	denotes	activities
T7611	31889-31890	.	denotes	.
T7612	31890-32184	sentence	denotes	These will be important for implicating candidates to be assessed in the extraembryonic signals that determine anterior streak progression in the developing embryo and in delineating the dominant activity of the polyglutamine tract in huntingtin that determines the striatal specificity of HD.
T7613	31891-31896	DT	denotes	These
T7615	31897-31901	MD	denotes	will
T7614	31902-31904	VB	denotes	be
T7616	31905-31914	JJ	denotes	important
T7617	31915-31918	IN	denotes	for
T7618	31919-31930	VBG	denotes	implicating
T7619	31931-31941	NNS	denotes	candidates
T7620	31942-31944	TO	denotes	to
T7622	31945-31947	VB	denotes	be
T7621	31948-31956	VBN	denotes	assessed
T7623	31957-31959	IN	denotes	in
T7624	31960-31963	DT	denotes	the
T7626	31964-31978	JJ	denotes	extraembryonic
T7625	31979-31986	NNS	denotes	signals
T7627	31987-31991	WDT	denotes	that
T7628	31992-32001	VBP	denotes	determine
T7629	32002-32010	JJ	denotes	anterior
T7630	32011-32017	NN	denotes	streak
T7631	32018-32029	NN	denotes	progression
T7632	32030-32032	IN	denotes	in
T7633	32033-32036	DT	denotes	the
T7635	32037-32047	VBG	denotes	developing
T7634	32048-32054	NN	denotes	embryo
T7636	32055-32058	CC	denotes	and
T7637	32059-32061	IN	denotes	in
T7638	32062-32073	VBG	denotes	delineating
T7639	32074-32077	DT	denotes	the
T7641	32078-32086	JJ	denotes	dominant
T7640	32087-32095	NN	denotes	activity
T7642	32096-32098	IN	denotes	of
T7643	32099-32102	DT	denotes	the
T7645	32103-32116	NN	denotes	polyglutamine
T7644	32117-32122	NN	denotes	tract
T7646	32123-32125	IN	denotes	in
T7647	32126-32136	NN	denotes	huntingtin
T7648	32137-32141	WDT	denotes	that
T7649	32142-32152	VBZ	denotes	determines
T7650	32153-32156	DT	denotes	the
T7652	32157-32165	JJ	denotes	striatal
T7651	32166-32177	NN	denotes	specificity
T7653	32178-32180	IN	denotes	of
T7654	32181-32183	NN	denotes	HD
T7655	32183-32184	.	denotes	.
T7725	32195-32199	NNS	denotes	Mice
T7726	32200-32203	CC	denotes	and
T7727	32204-32214	NN	denotes	genotyping
T7728	32214-32362	sentence	denotes	The Hdhex4/5 mice carrying a pGKneo insertion/replacement inactivating mutation in the mouse HD gene homologue have been described previously [10].
T7729	32215-32218	DT	denotes	The
T7731	32219-32225	NN	denotes	Hdhex4
T7732	32225-32226	HYPH	denotes	/
T7733	32226-32227	CD	denotes	5
T7730	32228-32232	NNS	denotes	mice
T7735	32233-32241	VBG	denotes	carrying
T7736	32242-32243	DT	denotes	a
T7738	32244-32250	NN	denotes	pGKneo
T7740	32251-32260	NN	denotes	insertion
T7741	32260-32261	HYPH	denotes	/
T7739	32261-32272	NN	denotes	replacement
T7742	32273-32285	VBG	denotes	inactivating
T7737	32286-32294	NN	denotes	mutation
T7743	32295-32297	IN	denotes	in
T7744	32298-32301	DT	denotes	the
T7746	32302-32307	NN	denotes	mouse
T7747	32308-32310	NN	denotes	HD
T7748	32311-32315	NN	denotes	gene
T7745	32316-32325	NN	denotes	homologue
T7749	32326-32330	VBP	denotes	have
T7750	32331-32335	VBN	denotes	been
T7734	32336-32345	VBN	denotes	described
T7751	32346-32356	RB	denotes	previously
T7752	32357-32358	-LRB-	denotes	[
T7753	32358-32360	CD	denotes	10
T7754	32360-32361	-RRB-	denotes	]
T7755	32361-32362	.	denotes	.
T7756	32362-32489	sentence	denotes	The experiments were conducted in accordance with an IACUC approved protocol, through the MGH Subcommittee on Animal Research.
T7757	32363-32366	DT	denotes	The
T7758	32367-32378	NNS	denotes	experiments
T7760	32379-32383	VBD	denotes	were
T7759	32384-32393	VBN	denotes	conducted
T7761	32394-32396	IN	denotes	in
T7762	32397-32407	NN	denotes	accordance
T7763	32408-32412	IN	denotes	with
T7764	32413-32415	DT	denotes	an
T7766	32416-32421	NNP	denotes	IACUC
T7767	32422-32430	VBN	denotes	approved
T7765	32431-32439	NN	denotes	protocol
T7768	32439-32441	,	denotes	,
T7769	32441-32448	IN	denotes	through
T7770	32449-32452	DT	denotes	the
T7772	32453-32456	NNP	denotes	MGH
T7771	32457-32469	NNP	denotes	Subcommittee
T7773	32470-32472	IN	denotes	on
T7774	32473-32479	NNP	denotes	Animal
T7775	32480-32488	NNP	denotes	Research
T7776	32488-32489	.	denotes	.
T7777	32489-32659	sentence	denotes	Mutant Hdhex4/5/Hdhex4/5 and normal littermates were obtained in timed pregnancies from mating of Hdhex4/5/Hdh+ heterozygotes, genotyped by PCR assay, as described [10].
T7778	32490-32496	NN	denotes	Mutant
T7779	32497-32503	NN	denotes	Hdhex4
T7781	32503-32504	HYPH	denotes	/
T7782	32504-32505	CD	denotes	5
T7783	32505-32506	HYPH	denotes	/
T7784	32506-32512	NN	denotes	Hdhex4
T7785	32512-32513	HYPH	denotes	/
T7786	32513-32514	CD	denotes	5
T7787	32515-32518	CC	denotes	and
T7788	32519-32525	JJ	denotes	normal
T7780	32526-32537	NNS	denotes	littermates
T7790	32538-32542	VBD	denotes	were
T7789	32543-32551	VBN	denotes	obtained
T7791	32552-32554	IN	denotes	in
T7792	32555-32560	VBN	denotes	timed
T7793	32561-32572	NNS	denotes	pregnancies
T7794	32573-32577	IN	denotes	from
T7795	32578-32584	NN	denotes	mating
T7796	32585-32587	IN	denotes	of
T7797	32588-32594	NN	denotes	Hdhex4
T7799	32594-32595	HYPH	denotes	/
T7800	32595-32596	CD	denotes	5
T7801	32596-32597	HYPH	denotes	/
T7802	32597-32600	NN	denotes	Hdh
T7803	32600-32601	SYM	denotes	+
T7798	32602-32615	NNS	denotes	heterozygotes
T7804	32615-32617	,	denotes	,
T7805	32617-32626	VBN	denotes	genotyped
T7806	32627-32629	IN	denotes	by
T7807	32630-32633	NN	denotes	PCR
T7808	32634-32639	NN	denotes	assay
T7809	32639-32641	,	denotes	,
T7810	32641-32643	IN	denotes	as
T7811	32644-32653	VBN	denotes	described
T7812	32654-32655	-LRB-	denotes	[
T7813	32655-32657	CD	denotes	10
T7814	32657-32658	-RRB-	denotes	]
T7815	32658-32659	.	denotes	.
T7816	32659-32697	sentence	denotes	The day of plug was taken to be E0.5.
T7817	32660-32663	DT	denotes	The
T7818	32664-32667	NN	denotes	day
T7820	32668-32670	IN	denotes	of
T7821	32671-32675	NN	denotes	plug
T7822	32676-32679	VBD	denotes	was
T7819	32680-32685	VBN	denotes	taken
T7823	32686-32688	TO	denotes	to
T7824	32689-32691	VB	denotes	be
T7825	32692-32696	NN	denotes	E0.5
T7826	32696-32697	.	denotes	.
T7827	32697-32811	sentence	denotes	Embryos that were morphologically normal were pooled separately from morphologically mutant embryos for analysis.
T7828	32698-32705	NNS	denotes	Embryos
T7830	32706-32710	WDT	denotes	that
T7831	32711-32715	VBD	denotes	were
T7832	32716-32731	RB	denotes	morphologically
T7833	32732-32738	JJ	denotes	normal
T7834	32739-32743	VBD	denotes	were
T7829	32744-32750	VBN	denotes	pooled
T7835	32751-32761	RB	denotes	separately
T7836	32762-32766	IN	denotes	from
T7837	32767-32782	RB	denotes	morphologically
T7838	32783-32789	NN	denotes	mutant
T7839	32790-32797	NNS	denotes	embryos
T7840	32798-32801	IN	denotes	for
T7841	32802-32810	NN	denotes	analysis
T7842	32810-32811	.	denotes	.
T7843	32811-32966	sentence	denotes	Nodal expression was determined in embryos from matings of Hdhex4/5/Hdh+; NdllacZ/Ndl+ compound heterozygotes genotyped by PCR assay as described in [29].
T7844	32812-32817	NN	denotes	Nodal
T7845	32818-32828	NN	denotes	expression
T7847	32829-32832	VBD	denotes	was
T7846	32833-32843	VBN	denotes	determined
T7848	32844-32846	IN	denotes	in
T7849	32847-32854	NNS	denotes	embryos
T7850	32855-32859	IN	denotes	from
T7851	32860-32867	NNS	denotes	matings
T7852	32868-32870	IN	denotes	of
T7853	32871-32877	NN	denotes	Hdhex4
T7855	32877-32878	HYPH	denotes	/
T7856	32878-32879	CD	denotes	5
T7857	32879-32880	HYPH	denotes	/
T7858	32880-32883	NN	denotes	Hdh
T7859	32883-32884	SYM	denotes	+
T7860	32884-32885	,	denotes	;
T7861	32886-32893	NN	denotes	NdllacZ
T7863	32893-32894	HYPH	denotes	/
T7862	32894-32897	NN	denotes	Ndl
T7864	32897-32898	SYM	denotes	+
T7865	32899-32907	NN	denotes	compound
T7854	32908-32921	NNS	denotes	heterozygotes
T7866	32922-32931	VBN	denotes	genotyped
T7867	32932-32934	IN	denotes	by
T7868	32935-32938	NN	denotes	PCR
T7869	32939-32944	NN	denotes	assay
T7870	32945-32947	IN	denotes	as
T7871	32948-32957	VBN	denotes	described
T7872	32958-32960	IN	denotes	in
T7873	32961-32962	-LRB-	denotes	[
T7874	32962-32964	CD	denotes	29
T7875	32964-32965	-RRB-	denotes	]
T7876	32965-32966	.	denotes	.
T7923	32968-32973	JJ	denotes	Whole
T7924	32974-32979	NN	denotes	mount
T7926	32980-32983	CC	denotes	and
T7927	32984-32991	NN	denotes	section
T7928	32992-32994	FW	denotes	in
T7929	32995-32999	FW	denotes	situ
T7925	33000-33013	NN	denotes	hybridization
T7930	33014-33017	CC	denotes	and
T7931	33018-33019	NN	denotes	β
T7933	33019-33020	HYPH	denotes	-
T7932	33020-33023	NN	denotes	gal
T7934	33024-33032	NN	denotes	staining
T7935	33032-33117	sentence	denotes	After dissection in PBS, embryos were fixed overnight in 4% paraformaldehyde at 4°C.
T7936	33033-33038	IN	denotes	After
T7938	33039-33049	NN	denotes	dissection
T7939	33050-33052	IN	denotes	in
T7940	33053-33056	NN	denotes	PBS
T7941	33056-33058	,	denotes	,
T7942	33058-33065	NNS	denotes	embryos
T7943	33066-33070	VBD	denotes	were
T7937	33071-33076	VBN	denotes	fixed
T7944	33077-33086	RB	denotes	overnight
T7945	33087-33089	IN	denotes	in
T7946	33090-33091	CD	denotes	4
T7947	33091-33092	NN	denotes	%
T7948	33093-33109	NN	denotes	paraformaldehyde
T7949	33110-33112	IN	denotes	at
T7950	33113-33114	CD	denotes	4
T7951	33114-33116	NN	denotes	°C
T7952	33116-33117	.	denotes	.
T7953	33117-33223	sentence	denotes	For sections, decidua fixed in 4% paraformaldehyde, were embedded in paraffin and sectioned at 7 microns.
T7954	33118-33121	IN	denotes	For
T7956	33122-33130	NNS	denotes	sections
T7957	33130-33132	,	denotes	,
T7958	33132-33139	NNS	denotes	decidua
T7959	33140-33145	VBN	denotes	fixed
T7960	33146-33148	IN	denotes	in
T7961	33149-33150	CD	denotes	4
T7962	33150-33151	NN	denotes	%
T7963	33152-33168	NN	denotes	paraformaldehyde
T7964	33168-33170	,	denotes	,
T7965	33170-33174	VBD	denotes	were
T7955	33175-33183	VBN	denotes	embedded
T7966	33184-33186	IN	denotes	in
T7967	33187-33195	NN	denotes	paraffin
T7968	33196-33199	CC	denotes	and
T7969	33200-33209	VBN	denotes	sectioned
T7970	33210-33212	IN	denotes	at
T7971	33213-33214	CD	denotes	7
T7972	33215-33222	NNS	denotes	microns
T7973	33222-33223	.	denotes	.
T7974	33223-33295	sentence	denotes	RNA in situ hybridizations were performed as described previously [55].
T7975	33224-33227	NN	denotes	RNA
T7977	33228-33230	FW	denotes	in
T7978	33231-33235	FW	denotes	situ
T7976	33236-33250	NNS	denotes	hybridizations
T7980	33251-33255	VBD	denotes	were
T7979	33256-33265	VBN	denotes	performed
T7981	33266-33268	IN	denotes	as
T7982	33269-33278	VBN	denotes	described
T7983	33279-33289	RB	denotes	previously
T7984	33290-33291	-LRB-	denotes	[
T7985	33291-33293	CD	denotes	55
T7986	33293-33294	-RRB-	denotes	]
T7987	33294-33295	.	denotes	.
T7988	33295-33422	sentence	denotes	Nodal.lacZ expression was assessed by β-galactosidase staining as reported [29], on embryos post fixed in 4% paraformaldehyde.
T7989	33296-33301	NN	denotes	Nodal
T7991	33301-33302	HYPH	denotes	.
T7990	33302-33306	NN	denotes	lacZ
T7992	33307-33317	NN	denotes	expression
T7994	33318-33321	VBD	denotes	was
T7993	33322-33330	VBN	denotes	assessed
T7995	33331-33333	IN	denotes	by
T7996	33334-33335	NN	denotes	β
T7998	33335-33336	HYPH	denotes	-
T7997	33336-33349	NN	denotes	galactosidase
T7999	33350-33358	NN	denotes	staining
T8000	33359-33361	IN	denotes	as
T8001	33362-33370	VBN	denotes	reported
T8002	33371-33372	-LRB-	denotes	[
T8003	33372-33374	CD	denotes	29
T8004	33374-33375	-RRB-	denotes	]
T8005	33375-33377	,	denotes	,
T8006	33377-33379	IN	denotes	on
T8007	33380-33387	NNS	denotes	embryos
T8008	33388-33392	AFX	denotes	post
T8009	33393-33398	VBN	denotes	fixed
T8010	33399-33401	IN	denotes	in
T8011	33402-33403	CD	denotes	4
T8012	33403-33404	NN	denotes	%
T8013	33405-33421	NN	denotes	paraformaldehyde
T8014	33421-33422	.	denotes	.
T8015	33422-33486	sentence	denotes	Embryos were mounted in 80% glycerol before being photographed.
T8016	33423-33430	NNS	denotes	Embryos
T8018	33431-33435	VBD	denotes	were
T8017	33436-33443	VBN	denotes	mounted
T8019	33444-33446	IN	denotes	in
T8020	33447-33449	CD	denotes	80
T8021	33449-33450	NN	denotes	%
T8022	33451-33459	NN	denotes	glycerol
T8023	33460-33466	IN	denotes	before
T8024	33467-33472	VBG	denotes	being
T8025	33473-33485	VBN	denotes	photographed
T8026	33485-33486	.	denotes	.
T8027	33486-33587	sentence	denotes	The huntingtin deficient phenotype is fully penetrant at each of the stages that were assessed [10].
T8028	33487-33490	DT	denotes	The
T8030	33491-33501	NN	denotes	huntingtin
T8031	33502-33511	JJ	denotes	deficient
T8029	33512-33521	NN	denotes	phenotype
T8032	33522-33524	VBZ	denotes	is
T8033	33525-33530	RB	denotes	fully
T8034	33531-33540	JJ	denotes	penetrant
T8035	33541-33543	IN	denotes	at
T8036	33544-33548	DT	denotes	each
T8037	33549-33551	IN	denotes	of
T8038	33552-33555	DT	denotes	the
T8039	33556-33562	NNS	denotes	stages
T8040	33563-33567	WDT	denotes	that
T8042	33568-33572	VBD	denotes	were
T8041	33573-33581	VBN	denotes	assessed
T8043	33582-33583	-LRB-	denotes	[
T8044	33583-33585	CD	denotes	10
T8045	33585-33586	-RRB-	denotes	]
T8046	33586-33587	.	denotes	.
T8047	33587-33709	sentence	denotes	Three to six embryos were evaluated for each marker, with every embryo exhibiting the same mutant phenotype in each case.
T8048	33588-33593	CD	denotes	Three
T8050	33594-33596	IN	denotes	to
T8049	33597-33600	CD	denotes	six
T8051	33601-33608	NNS	denotes	embryos
T8053	33609-33613	VBD	denotes	were
T8052	33614-33623	VBN	denotes	evaluated
T8054	33624-33627	IN	denotes	for
T8055	33628-33632	DT	denotes	each
T8056	33633-33639	NN	denotes	marker
T8057	33639-33641	,	denotes	,
T8058	33641-33645	IN	denotes	with
T8059	33646-33651	DT	denotes	every
T8060	33652-33658	NN	denotes	embryo
T8061	33659-33669	VBG	denotes	exhibiting
T8062	33670-33673	DT	denotes	the
T8064	33674-33678	JJ	denotes	same
T8065	33679-33685	NN	denotes	mutant
T8063	33686-33695	NN	denotes	phenotype
T8066	33696-33698	IN	denotes	in
T8067	33699-33703	DT	denotes	each
T8068	33704-33708	NN	denotes	case
T8069	33708-33709	.	denotes	.
T8126	33711-33719	NN	denotes	Alkaline
T8127	33720-33731	NN	denotes	phosphatase
T8128	33732-33740	NN	denotes	staining
T8129	33741-33743	IN	denotes	of
T8130	33744-33754	JJ	denotes	Primordial
T8132	33755-33759	NN	denotes	Germ
T8131	33760-33765	NNS	denotes	Cells
T8133	33766-33767	-LRB-	denotes	(
T8134	33767-33771	NNS	denotes	PCGs
T8135	33771-33772	-RRB-	denotes	)
T8136	33772-33894	sentence	denotes	After dissections, embryos were fixed in 4% paraformaldehyde briefly and washed and stored in 1 × PBS/0.1% TX-100 at 4°C.
T8137	33773-33778	IN	denotes	After
T8139	33779-33790	NNS	denotes	dissections
T8140	33790-33792	,	denotes	,
T8141	33792-33799	NNS	denotes	embryos
T8142	33800-33804	VBD	denotes	were
T8138	33805-33810	VBN	denotes	fixed
T8143	33811-33813	IN	denotes	in
T8144	33814-33815	CD	denotes	4
T8145	33815-33816	NN	denotes	%
T8146	33817-33833	NN	denotes	paraformaldehyde
T8147	33834-33841	RB	denotes	briefly
T8148	33842-33845	CC	denotes	and
T8149	33846-33852	VBN	denotes	washed
T8150	33853-33856	CC	denotes	and
T8151	33857-33863	VBN	denotes	stored
T8152	33864-33866	IN	denotes	in
T8153	33867-33868	CD	denotes	1
T8155	33869-33870	SYM	denotes	×
T8154	33871-33874	NN	denotes	PBS
T8156	33874-33875	HYPH	denotes	/
T8157	33875-33878	NN	denotes	0.1
T8158	33878-33879	NN	denotes	%
T8159	33880-33882	NN	denotes	TX
T8160	33882-33883	HYPH	denotes	-
T8161	33883-33886	CD	denotes	100
T8162	33887-33889	IN	denotes	at
T8163	33890-33891	CD	denotes	4
T8164	33891-33893	NN	denotes	°C
T8165	33893-33894	.	denotes	.
T8166	33894-34163	sentence	denotes	Embryos were washed once with Tris-Maleate Buffer (25 mM Tris-Maleate, pH = 9.0, 0.8 mM MgCl2) and were subsequently incubated in alkaline phosphatase staining solution (25 mM Tris-Maleate, pH = 9.0, 0.8 mM MgCl2, 0.4 mg/ml alpha-naphthyl phosphate, 1 mg/ml Fast-Red).
T8167	33895-33902	NNS	denotes	Embryos
T8169	33903-33907	VBD	denotes	were
T8168	33908-33914	VBN	denotes	washed
T8170	33915-33919	RB	denotes	once
T8171	33920-33924	IN	denotes	with
T8172	33925-33929	NN	denotes	Tris
T8174	33929-33930	HYPH	denotes	-
T8173	33930-33937	NN	denotes	Maleate
T8175	33938-33944	NN	denotes	Buffer
T8176	33945-33946	-LRB-	denotes	(
T8178	33946-33948	CD	denotes	25
T8179	33949-33951	NN	denotes	mM
T8181	33952-33956	NN	denotes	Tris
T8182	33956-33957	HYPH	denotes	-
T8180	33957-33964	NN	denotes	Maleate
T8183	33964-33966	,	denotes	,
T8184	33966-33968	NN	denotes	pH
T8186	33969-33970	SYM	denotes	=
T8185	33971-33974	CD	denotes	9.0
T8187	33974-33976	,	denotes	,
T8188	33976-33979	CD	denotes	0.8
T8189	33980-33982	NN	denotes	mM
T8177	33983-33988	NN	denotes	MgCl2
T8190	33988-33989	-RRB-	denotes	)
T8191	33990-33993	CC	denotes	and
T8192	33994-33998	VBD	denotes	were
T8194	33999-34011	RB	denotes	subsequently
T8193	34012-34021	VBN	denotes	incubated
T8195	34022-34024	IN	denotes	in
T8196	34025-34033	NN	denotes	alkaline
T8197	34034-34045	NN	denotes	phosphatase
T8199	34046-34054	NN	denotes	staining
T8198	34055-34063	NN	denotes	solution
T8200	34064-34065	-LRB-	denotes	(
T8202	34065-34067	CD	denotes	25
T8203	34068-34070	NN	denotes	mM
T8205	34071-34075	NN	denotes	Tris
T8206	34075-34076	HYPH	denotes	-
T8204	34076-34083	NN	denotes	Maleate
T8207	34083-34085	,	denotes	,
T8208	34085-34087	NN	denotes	pH
T8210	34088-34089	SYM	denotes	=
T8209	34090-34093	CD	denotes	9.0
T8211	34093-34095	,	denotes	,
T8212	34095-34098	CD	denotes	0.8
T8213	34099-34101	NN	denotes	mM
T8214	34102-34107	NN	denotes	MgCl2
T8215	34107-34109	,	denotes	,
T8216	34109-34112	CD	denotes	0.4
T8217	34113-34115	NN	denotes	mg
T8219	34115-34116	SYM	denotes	/
T8220	34116-34118	NN	denotes	ml
T8221	34119-34124	NN	denotes	alpha
T8223	34124-34125	HYPH	denotes	-
T8222	34125-34133	NN	denotes	naphthyl
T8218	34134-34143	NN	denotes	phosphate
T8224	34143-34145	,	denotes	,
T8225	34145-34146	CD	denotes	1
T8201	34147-34149	NN	denotes	mg
T8226	34149-34150	SYM	denotes	/
T8227	34150-34152	NN	denotes	ml
T8228	34153-34157	JJ	denotes	Fast
T8229	34157-34158	HYPH	denotes	-
T8230	34158-34161	JJ	denotes	Red
T8231	34161-34162	-RRB-	denotes	)
T8232	34162-34163	.	denotes	.
T8233	34163-34215	sentence	denotes	Stained embryos were washed in 1 × PBS/0.1% TX-100.
T8234	34164-34171	VBN	denotes	Stained
T8235	34172-34179	NNS	denotes	embryos
T8237	34180-34184	VBD	denotes	were
T8236	34185-34191	VBN	denotes	washed
T8238	34192-34194	IN	denotes	in
T8239	34195-34196	CD	denotes	1
T8241	34197-34198	SYM	denotes	×
T8240	34199-34202	NN	denotes	PBS
T8242	34202-34203	SYM	denotes	/
T8244	34203-34206	CD	denotes	0.1
T8245	34206-34207	NN	denotes	%
T8243	34208-34210	NN	denotes	TX
T8246	34210-34211	HYPH	denotes	-
T8247	34211-34214	CD	denotes	100
T8248	34214-34215	.	denotes	.
T8266	34217-34222	JJ	denotes	Whole
T8267	34223-34229	NN	denotes	embryo
T8268	34230-34237	NN	denotes	culture
T8269	34237-34288	sentence	denotes	Embryos were dissected at E7.5 and washed in DMEM.
T8270	34238-34245	NNS	denotes	Embryos
T8272	34246-34250	VBD	denotes	were
T8271	34251-34260	VBN	denotes	dissected
T8273	34261-34263	IN	denotes	at
T8274	34264-34268	NN	denotes	E7.5
T8275	34269-34272	CC	denotes	and
T8276	34273-34279	VBN	denotes	washed
T8277	34280-34282	IN	denotes	in
T8278	34283-34287	NN	denotes	DMEM
T8279	34287-34288	.	denotes	.
T8280	34288-34470	sentence	denotes	Embryos were then cultured individually in 1 ml of culture media (75% immediately centrifuged rat serum and 25% DMEM [56]) for 24 hours while rotating in a 37°C incubator in 5% CO2.
T8281	34289-34296	NNS	denotes	Embryos
T8283	34297-34301	VBD	denotes	were
T8284	34302-34306	RB	denotes	then
T8282	34307-34315	VBN	denotes	cultured
T8285	34316-34328	RB	denotes	individually
T8286	34329-34331	IN	denotes	in
T8287	34332-34333	CD	denotes	1
T8288	34334-34336	NN	denotes	ml
T8289	34337-34339	IN	denotes	of
T8290	34340-34347	NN	denotes	culture
T8291	34348-34353	NNS	denotes	media
T8292	34354-34355	-LRB-	denotes	(
T8293	34355-34357	CD	denotes	75
T8294	34357-34358	NN	denotes	%
T8296	34359-34370	RB	denotes	immediately
T8297	34371-34382	VBN	denotes	centrifuged
T8298	34383-34386	NN	denotes	rat
T8295	34387-34392	NN	denotes	serum
T8299	34393-34396	CC	denotes	and
T8300	34397-34399	CD	denotes	25
T8301	34399-34400	NN	denotes	%
T8302	34401-34405	NN	denotes	DMEM
T8303	34406-34407	-LRB-	denotes	[
T8304	34407-34409	CD	denotes	56
T8305	34409-34410	-RRB-	denotes	]
T8306	34410-34411	-RRB-	denotes	)
T8307	34412-34415	IN	denotes	for
T8308	34416-34418	CD	denotes	24
T8309	34419-34424	NNS	denotes	hours
T8310	34425-34430	IN	denotes	while
T8311	34431-34439	VBG	denotes	rotating
T8312	34440-34442	IN	denotes	in
T8313	34443-34444	DT	denotes	a
T8315	34445-34447	CD	denotes	37
T8316	34447-34449	NN	denotes	°C
T8314	34450-34459	NN	denotes	incubator
T8317	34460-34462	IN	denotes	in
T8318	34463-34464	CD	denotes	5
T8319	34464-34465	NN	denotes	%
T8320	34466-34469	NN	denotes	CO2
T8321	34469-34470	.	denotes	.
T8322	34470-34531	sentence	denotes	Embryos were then fixed in 4% paraformaldehyde for analysis.
T8323	34471-34478	NNS	denotes	Embryos
T8325	34479-34483	VBD	denotes	were
T8326	34484-34488	RB	denotes	then
T8324	34489-34494	VBN	denotes	fixed
T8327	34495-34497	IN	denotes	in
T8328	34498-34499	CD	denotes	4
T8329	34499-34500	NN	denotes	%
T8330	34501-34517	NN	denotes	paraformaldehyde
T8331	34518-34521	IN	denotes	for
T8332	34522-34530	NN	denotes	analysis
T8333	34530-34531	.	denotes	.
R10	T213	T209	punct	(,gene
R100	T314	T315	punct	–,7.5
R1000	T1631	T1632	nmod	growth,factors
R1001	T1632	T1630	pobj	factors,of
R1002	T1633	T1631	cc	and,growth
R1003	T1634	T1631	conj	transcription,growth
R1004	T1635	T1604	punct	.,support
R101	T315	T312	prep	7.5,E7.0
R1019	T2201	T2202	npadvmod	Huntingtin,deficient
R102	T316	T300	conj	exhibit,appears
R1020	T2202	T2204	amod	deficient,embryos
R1021	T2203	T2202	punct	-,deficient
R1022	T2204	T2205	nsubj	embryos,exhibit
R1023	T2206	T2207	amod	abnormal,progression
R1024	T2207	T2205	dobj	progression,exhibit
R1025	T2208	T2207	compound	streak,progression
R1026	T2209	T2207	cc	and,progression
R1027	T2210	T2211	amod	paraxial,production
R1028	T2211	T2207	conj	production,progression
R1029	T2212	T2211	compound	mesoderm,production
R103	T317	T318	det	a,combination
R1030	T2214	T2215	mark	Since,supply
R1031	T2215	T2218	advcl	supply,tested
R1032	T2216	T2217	amod	extraembryonic,tissues
R1033	T2217	T2215	nsubj	tissues,supply
R1034	T2219	T2215	dobj	nutrients,supply
R1035	T2220	T2215	prep	to,supply
R1036	T2221	T2222	det	the,embryo
R1037	T2222	T2220	pobj	embryo,to
R1038	T2223	T2222	amod	developing,embryo
R1039	T2224	T2218	punct	", ",tested
R104	T318	T316	dobj	combination,exhibit
R1040	T2225	T2218	nsubj	we,tested
R1041	T2226	T2227	det	the,possibility
R1042	T2227	T2218	dobj	possibility,tested
R1043	T2228	T2229	mark	that,perturb
R1044	T2229	T2227	acl	perturb,possibility
R1045	T2230	T2231	compound	huntingtin,deficiency
R1046	T2231	T2229	nsubj	deficiency,perturb
R1047	T2232	T2229	aux	may,perturb
R1048	T2233	T2234	det	this,function
R1049	T2234	T2229	dobj	function,perturb
R105	T319	T318	amod	unique,combination
R1050	T2235	T2229	prep	by,perturb
R1051	T2236	T2235	pcomp	performing,by
R1052	T2237	T2238	compound	RT,PCR
R1053	T2238	T2240	compound	PCR,analysis
R1054	T2239	T2238	punct	-,PCR
R1055	T2240	T2236	dobj	analysis,performing
R1056	T2241	T2242	aux	to,examine
R1057	T2242	T2236	advcl	examine,performing
R1058	T2243	T2244	det	the,expression
R1059	T2244	T2242	dobj	expression,examine
R106	T320	T318	prep	of,combination
R1060	T2245	T2244	prep	of,expression
R1061	T2246	T2247	det	a,panel
R1062	T2247	T2245	pobj	panel,of
R1063	T2248	T2247	prep	of,panel
R1064	T2249	T2248	punct	',of
R1065	T2250	T2251	amod	nutritive,genes
R1066	T2251	T2248	pobj	genes,of
R1067	T2252	T2251	punct	',genes
R1068	T2253	T2244	prep	in,expression
R1069	T2254	T2255	nmod	E7.5,embryos
R107	T321	T322	compound	patterning,defects
R1070	T2255	T2253	pobj	embryos,in
R1071	T2256	T2257	amod	wild,type
R1072	T2257	T2255	nmod	type,embryos
R1073	T2258	T2257	punct	-,type
R1074	T2259	T2257	cc	and,type
R1075	T2260	T2257	conj	Hdhex4,type
R1076	T2261	T2260	punct	/,Hdhex4
R1077	T2262	T2260	nummod	5,Hdhex4
R1078	T2263	T2260	punct	/,Hdhex4
R1079	T2264	T2260	appos	Hdhex4,Hdhex4
R108	T322	T320	pobj	defects,of
R1080	T2265	T2264	punct	/,Hdhex4
R1081	T2266	T2264	nummod	5,Hdhex4
R1082	T2267	T2268	npadvmod	huntingtin,deficient
R1083	T2268	T2255	amod	deficient,embryos
R1084	T2269	T2218	punct	.,tested
R1085	T2271	T2272	advcl	Consistent,found
R1086	T2273	T2271	prep	with,Consistent
R1087	T2274	T2275	det	a,report
R1088	T2275	T2273	pobj	report,with
R1089	T2276	T2275	amod	previous,report
R109	T323	T316	punct	.,exhibit
R1090	T2277	T2278	punct	[,16
R1091	T2278	T2271	parataxis	16,Consistent
R1092	T2279	T2278	punct	],16
R1093	T2280	T2272	punct	", ",found
R1094	T2281	T2282	det	no,differences
R1095	T2282	T2272	nsubjpass	differences,found
R1096	T2283	T2282	amod	obvious,differences
R1097	T2284	T2272	auxpass	were,found
R1098	T2285	T2272	prep	in,found
R1099	T2286	T2287	det	the,expression
R11	T214	T209	appos	Hdh,gene
R110	T325	T326	acomp	Notable,are
R1100	T2287	T2285	pobj	expression,in
R1101	T2288	T2287	prep	of,expression
R1102	T2289	T2288	punct	"""",of
R1103	T2290	T2291	amod	nutritive,genes
R1104	T2291	T2288	pobj	genes,of
R1105	T2292	T2291	punct	"""",genes
R1106	T2293	T2291	punct	(,genes
R1107	T2294	T2291	appos	Hnf4,genes
R1108	T2295	T2294	punct	", ",Hnf4
R1109	T2296	T2294	conj	Afp,Hnf4
R111	T327	T328	det	a,streak
R1110	T2297	T2296	punct	", ",Afp
R1111	T2298	T2296	conj	Tfn,Afp
R1112	T2299	T2298	punct	", ",Tfn
R1113	T2300	T2298	conj	ApoAI,Tfn
R1114	T2301	T2300	punct	", ",ApoAI
R1115	T2302	T2303	compound	Apo,AIV
R1116	T2303	T2300	conj	AIV,ApoAI
R1117	T2304	T2303	punct	-,AIV
R1118	T2305	T2303	punct	", ",AIV
R1119	T2306	T2303	cc	and,AIV
R112	T328	T326	nsubj	streak,are
R1120	T2307	T2303	conj	ApoB,AIV
R1121	T2308	T2291	punct	),genes
R1122	T2309	T2291	cc	or,genes
R1123	T2310	T2291	conj	genes,genes
R1124	T2311	T2310	acl	involved,genes
R1125	T2312	T2311	prep	in,involved
R1126	T2313	T2314	compound	yolk,sac
R1127	T2314	T2315	compound	sac,hematopoiesis
R1128	T2315	T2312	pobj	hematopoiesis,in
R1129	T2316	T2315	cc	or,hematopoiesis
R113	T329	T328	amod	shortened,streak
R1130	T2317	T2315	conj	vasculogenesis,hematopoiesis
R1131	T2318	T2310	punct	(,genes
R1132	T2319	T2320	nmod	Ttr,GATA1
R1133	T2320	T2310	appos	GATA1,genes
R1134	T2321	T2320	punct	", ",GATA1
R1135	T2322	T2320	nmod	Rbp,GATA1
R1136	T2323	T2320	punct	", ",GATA1
R1137	T2324	T2320	nmod	Flt1,GATA1
R1138	T2325	T2320	punct	", ",GATA1
R1139	T2326	T2320	nmod	Flk1,GATA1
R114	T330	T328	amod	primitive,streak
R1140	T2327	T2320	punct	", ",GATA1
R1141	T2328	T2320	nmod	Tal1,GATA1
R1142	T2329	T2320	punct	", ",GATA1
R1143	T2330	T2320	nmod	Rbtn2,GATA1
R1144	T2331	T2320	punct	", ",GATA1
R1145	T2332	T2310	punct	),genes
R1146	T2333	T2334	punct	(,shown
R1147	T2334	T2310	parataxis	shown,genes
R1148	T2335	T2334	nsubj	data,shown
R1149	T2336	T2334	neg	not,shown
R115	T331	T328	punct	", ",streak
R1150	T2337	T2334	punct	),shown
R1151	T2338	T2272	punct	", ",found
R1152	T2339	T2272	advcl	suggesting,found
R1153	T2340	T2341	mark	that,is
R1154	T2341	T2339	ccomp	is,suggesting
R1155	T2342	T2341	nsubj	huntingtin,is
R1156	T2343	T2341	neg	not,is
R1157	T2344	T2341	acomp	essential,is
R1158	T2345	T2344	prep	for,essential
R1159	T2346	T2347	det	the,expression
R116	T332	T328	conj	absence,streak
R1160	T2347	T2345	pobj	expression,for
R1161	T2348	T2347	amod	proper,expression
R1162	T2349	T2347	prep	of,expression
R1163	T2350	T2349	pobj	genes,of
R1164	T2351	T2350	acl	required,genes
R1165	T2352	T2351	prep	for,required
R1166	T2353	T2354	det	the,function
R1167	T2354	T2352	pobj	function,for
R1168	T2355	T2354	amod	nutritive,function
R1169	T2356	T2354	prep	of,function
R117	T333	T332	prep	of,absence
R1170	T2357	T2358	det	the,tissues
R1171	T2358	T2356	pobj	tissues,of
R1172	T2359	T2358	amod	extraembryonic,tissues
R1173	T2360	T2272	punct	.,found
R1174	T2362	T2363	aux	To,investigate
R1175	T2363	T2364	advcl	investigate,analyzed
R1176	T2365	T2366	poss	huntingtin,activities
R1177	T2366	T2363	dobj	activities,investigate
R1178	T2367	T2365	case	's,huntingtin
R1179	T2368	T2366	amod	developmental,activities
R118	T334	T335	det	a,node
R1180	T2369	T2364	punct	", ",analyzed
R1181	T2370	T2364	nsubj	we,analyzed
R1182	T2371	T2364	advmod	then,analyzed
R1183	T2372	T2373	det	the,expression
R1184	T2373	T2364	dobj	expression,analyzed
R1185	T2374	T2373	prep	of,expression
R1186	T2375	T2374	pobj	genes,of
R1187	T2376	T2377	dep	which,pattern
R1188	T2377	T2375	relcl	pattern,genes
R1189	T2378	T2379	det	the,embryo
R119	T335	T333	pobj	node,of
R1190	T2379	T2377	dobj	embryo,pattern
R1191	T2380	T2379	amod	early,embryo
R1192	T2381	T2377	cc	or,pattern
R1193	T2382	T2377	conj	mark,pattern
R1194	T2383	T2384	amod	morphogenic,landmarks
R1195	T2384	T2382	dobj	landmarks,mark
R1196	T2385	T2382	prep	in,mark
R1197	T2386	T2387	amod	wild,type
R1198	T2387	T2389	nmod	type,embryos
R1199	T2388	T2387	punct	-,type
R12	T215	T205	punct	),impairs
R120	T336	T335	amod	proper,node
R1200	T2389	T2385	pobj	embryos,in
R1201	T2390	T2387	cc	and,type
R1202	T2391	T2387	conj	Hdhex4,type
R1203	T2392	T2391	punct	/,Hdhex4
R1204	T2393	T2391	nummod	5,Hdhex4
R1205	T2394	T2391	punct	/,Hdhex4
R1206	T2395	T2391	appos	Hdhex4,Hdhex4
R1207	T2396	T2395	punct	/,Hdhex4
R1208	T2397	T2395	nummod	5,Hdhex4
R1209	T2398	T2382	prep	by,mark
R121	T337	T332	cc	and,absence
R1210	T2399	T2400	amod	whole,mount
R1211	T2400	T2401	nmod	mount,hybridization
R1212	T2401	T2398	pobj	hybridization,by
R1213	T2402	T2400	cc	and,mount
R1214	T2403	T2400	conj	section,mount
R1215	T2404	T2405	advmod	in,situ
R1216	T2405	T2401	amod	situ,hybridization
R1217	T2406	T2364	punct	.,analyzed
R1218	T2408	T2409	det	The,dissections
R1219	T2409	T2410	nsubj	dissections,confirmed
R122	T338	T339	amod	diminished,production
R1220	T2411	T2412	amod	previous,data
R1221	T2412	T2410	dobj	data,confirmed
R1222	T2413	T2412	amod	morphologic,data
R1223	T2414	T2412	prep	at,data
R1224	T2415	T2414	pobj	E7.0,at
R1225	T2416	T2417	punct	–,7.5
R1226	T2417	T2415	prep	7.5,E7.0
R1227	T2418	T2419	mark	that,exhibit
R1228	T2419	T2412	acl	exhibit,data
R1229	T2420	T2421	det	all,homozygotes
R123	T339	T332	conj	production,absence
R1230	T2421	T2419	nsubj	homozygotes,exhibit
R1231	T2422	T2421	nmod	Hdhex4,homozygotes
R1232	T2423	T2422	punct	/,Hdhex4
R1233	T2424	T2422	nummod	5,Hdhex4
R1234	T2425	T2422	punct	/,Hdhex4
R1235	T2426	T2422	appos	Hdhex4,Hdhex4
R1236	T2427	T2426	punct	/,Hdhex4
R1237	T2428	T2426	nummod	5,Hdhex4
R1238	T2429	T2430	amod	abnormal,morphology
R1239	T2430	T2419	dobj	morphology,exhibit
R124	T340	T339	prep	of,production
R1240	T2431	T2430	punct	", ",morphology
R1241	T2432	T2430	prep	including,morphology
R1242	T2433	T2434	amod	shortened,streak
R1243	T2434	T2432	pobj	streak,including
R1244	T2435	T2434	amod	primitive,streak
R1245	T2436	T2434	cc	and,streak
R1246	T2437	T2438	det	a,lack
R1247	T2438	T2434	conj	lack,streak
R1248	T2439	T2438	prep	of,lack
R1249	T2440	T2441	amod	morphological,folds
R125	T341	T342	amod	anterior,streak
R1250	T2441	T2439	pobj	folds,of
R1251	T2442	T2441	compound	head,folds
R1252	T2443	T2441	cc	or,folds
R1253	T2444	T2441	conj	node,folds
R1254	T2445	T2446	punct	[,13
R1255	T2446	T2419	parataxis	13,exhibit
R1256	T2447	T2446	nummod	10,13
R1257	T2448	T2446	punct	",",13
R1258	T2449	T2446	punct	],13
R1259	T2450	T2410	punct	.,confirmed
R126	T342	T343	compound	streak,derivatives
R1260	T2452	T2453	det	The,results
R1261	T2453	T2454	nsubj	results,confirmed
R1262	T2455	T2453	prep	of,results
R1263	T2456	T2457	advmod	in,situ
R1264	T2457	T2458	amod	situ,analysis
R1265	T2458	T2455	pobj	analysis,of
R1266	T2459	T2458	compound	hybridization,analysis
R1267	T2460	T2454	advmod	also,confirmed
R1268	T2461	T2462	mark	that,formed
R1269	T2462	T2454	ccomp	formed,confirmed
R127	T343	T340	pobj	derivatives,of
R1270	T2463	T2464	det	all,layers
R1271	T2464	T2462	nsubjpass	layers,formed
R1272	T2465	T2464	nummod	three,layers
R1273	T2466	T2464	compound	germ,layers
R1274	T2467	T2464	cc	and,layers
R1275	T2468	T2469	amod	extraembryonic,tissue
R1276	T2469	T2464	conj	tissue,layers
R1277	T2470	T2462	auxpass	are,formed
R1278	T2471	T2462	prep	in,formed
R1279	T2472	T2473	npadvmod	huntingtin,deficient
R128	T344	T326	punct	.,are
R1280	T2473	T2474	amod	deficient,embryos
R1281	T2474	T2471	pobj	embryos,in
R1282	T2475	T2454	punct	.,confirmed
R1283	T2477	T2478	nsubjpass	Otx2,expressed
R1284	T2479	T2477	punct	", ",Otx2
R1285	T2480	T2481	advmod	normally,expressed
R1286	T2481	T2477	acl	expressed,Otx2
R1287	T2482	T2481	prep	in,expressed
R1288	T2483	T2484	det	the,neuroectoderm
R1289	T2484	T2482	pobj	neuroectoderm,in
R129	T346	T347	amod	Reduced,expression
R1290	T2485	T2484	amod	anterior,neuroectoderm
R1291	T2486	T2484	cc	and,neuroectoderm
R1292	T2487	T2488	amod	anterior,endoderm
R1293	T2488	T2484	conj	endoderm,neuroectoderm
R1294	T2489	T2488	amod	visceral,endoderm
R1295	T2490	T2491	punct	[,19
R1296	T2491	T2481	parataxis	19,expressed
R1297	T2492	T2491	punct	],19
R1298	T2493	T2478	punct	", ",expressed
R1299	T2494	T2478	auxpass	is,expressed
R13	T216	T217	amod	anterior,formation
R130	T347	T353	nsubj	expression,signify
R1300	T2495	T2478	prep	in,expressed
R1301	T2496	T2497	compound	mutant,embryos
R1302	T2497	T2495	pobj	embryos,in
R1303	T2498	T2478	prep	at,expressed
R1304	T2499	T2498	pobj	E7.5,at
R1305	T2500	T2501	punct	(,B
R1306	T2501	T2499	parataxis	B,E7.5
R1307	T2502	T2501	nmod	Fig.,B
R1308	T2503	T2501	nmod	1A,B
R1309	T2504	T2501	punct	",",B
R131	T348	T347	nmod	Wnt3a,expression
R1310	T2505	T2501	punct	),B
R1311	T2506	T2507	mark	although,appears
R1312	T2507	T2478	advcl	appears,expressed
R1313	T2508	T2509	det	the,domain
R1314	T2509	T2507	nsubj	domain,appears
R1315	T2510	T2509	compound	expression,domain
R1316	T2511	T2507	oprd	reduced,appears
R1317	T2512	T2478	punct	.,expressed
R1318	T2514	T2515	advmod	Similarly,is
R1319	T2516	T2515	punct	", ",is
R132	T349	T348	punct	", ",Wnt3a
R1320	T2517	T2518	compound	Hesx1,expression
R1321	T2518	T2515	nsubj	expression,is
R1322	T2519	T2520	advmod	grossly,normal
R1323	T2520	T2515	acomp	normal,is
R1324	T2521	T2515	prep	in,is
R1325	T2522	T2523	compound	mutant,embryos
R1326	T2523	T2521	pobj	embryos,in
R1327	T2524	T2515	punct	", ",is
R1328	T2525	T2526	mark	with,localized
R1329	T2526	T2515	advcl	localized,is
R133	T350	T348	conj	Tbx6,Wnt3a
R1330	T2527	T2526	nsubj	expression,localized
R1331	T2528	T2526	prep	to,localized
R1332	T2529	T2530	det	the,AVE
R1333	T2530	T2528	pobj	AVE,to
R1334	T2531	T2530	cc	and,AVE
R1335	T2532	T2530	conj	neuroectoderm,AVE
R1336	T2533	T2534	punct	(,20
R1337	T2534	T2526	parataxis	20,localized
R1338	T2535	T2534	dep	Fig.,20
R1339	T2536	T2535	nummod	1C,Fig.
R134	T351	T350	cc	and,Tbx6
R1340	T2537	T2538	punct	–,F
R1341	T2538	T2535	prep	F,Fig.
R1342	T2539	T2534	punct	", ",20
R1343	T2540	T2534	punct	[,20
R1344	T2541	T2534	punct	],20
R1345	T2542	T2534	punct	),20
R1346	T2543	T2515	punct	", ",is
R1347	T2544	T2545	mark	although,appears
R1348	T2545	T2515	advcl	appears,is
R1349	T2546	T2547	det	the,domain
R135	T352	T350	conj	Dll1,Tbx6
R1350	T2547	T2545	nsubj	domain,appears
R1351	T2548	T2547	compound	expression,domain
R1352	T2549	T2545	advmod	also,appears
R1353	T2550	T2545	oprd	reduced,appears
R1354	T2551	T2515	punct	.,is
R1355	T2553	T2554	det	These,results
R1356	T2554	T2555	nsubj	results,indicate
R1357	T2556	T2557	amod	appropriate,specification
R1358	T2557	T2555	dobj	specification,indicate
R1359	T2558	T2557	cc	and,specification
R136	T354	T355	amod	decreased,mesoderm
R1360	T2559	T2557	conj	movement,specification
R1361	T2560	T2557	prep	of,specification
R1362	T2561	T2562	amod	anterior,endoderm
R1363	T2562	T2564	nmod	endoderm,cells
R1364	T2563	T2562	amod	visceral,endoderm
R1365	T2564	T2560	pobj	cells,of
R1366	T2565	T2562	punct	(,endoderm
R1367	T2566	T2562	appos	AVE,endoderm
R1368	T2567	T2564	punct	),cells
R1369	T2568	T2557	prep	from,specification
R137	T355	T353	dobj	mesoderm,signify
R1370	T2569	T2570	det	the,tip
R1371	T2570	T2568	pobj	tip,from
R1372	T2571	T2570	amod	distal,tip
R1373	T2572	T2555	cc	and,indicate
R1374	T2573	T2555	conj	suggest,indicate
R1375	T2574	T2575	mark	that,induced
R1376	T2575	T2573	ccomp	induced,suggest
R1377	T2576	T2575	nsubjpass	neuroectoderm,induced
R1378	T2577	T2575	auxpass	is,induced
R1379	T2578	T2575	prep	in,induced
R138	T356	T355	amod	paraxial,mesoderm
R1380	T2579	T2580	det	the,embryos
R1381	T2580	T2578	pobj	embryos,in
R1382	T2581	T2580	compound	mutant,embryos
R1383	T2582	T2555	punct	.,indicate
R1384	T2586	T2585	advmod	o,T
R1385	T2593	T2594	nsubjpass	m,i
R1386	T2598	T2599	advcl	e,analyzed
R1387	T2600	T2601	amod	finitive,formation
R1388	T2601	T2598	dobj	formation,e
R1389	T2602	T2601	compound	endoderm,formation
R139	T357	T355	cc	and,mesoderm
R1390	T2603	T2599	punct	", ",analyzed
R1391	T2604	T2605	det	the,expression
R1392	T2605	T2599	nsubjpass	expression,analyzed
R1393	T2606	T2605	prep	of,expression
R1394	T2607	T2606	pobj	Hnf3β,of
R1395	T2608	T2609	punct	(,FoxA2
R1396	T2609	T2607	parataxis	FoxA2,Hnf3β
R1397	T2610	T2609	punct	),FoxA2
R1398	T2611	T2605	prep	in,expression
R1399	T2612	T2613	nmod	mutant,embryos
R14	T217	T205	dobj	formation,impairs
R140	T358	T359	amod	reduced,expression
R1400	T2613	T2611	pobj	embryos,in
R1401	T2614	T2612	cc	and,mutant
R1402	T2615	T2616	amod	wild,type
R1403	T2616	T2612	conj	type,mutant
R1404	T2617	T2616	punct	-,type
R1405	T2618	T2599	auxpass	was,analyzed
R1406	T2619	T2599	punct	.,analyzed
R1407	T2621	T2622	prep	In,confined
R1408	T2623	T2624	amod	wild,type
R1409	T2624	T2626	compound	type,embryos
R141	T359	T355	conj	expression,mesoderm
R1410	T2625	T2624	punct	-,type
R1411	T2626	T2621	pobj	embryos,In
R1412	T2627	T2622	punct	", ",confined
R1413	T2628	T2629	compound	Hnf3β,expression
R1414	T2629	T2622	nsubjpass	expression,confined
R1415	T2630	T2622	auxpass	is,confined
R1416	T2631	T2622	prep	to,confined
R1417	T2632	T2633	det	the,node
R1418	T2633	T2631	pobj	node,to
R1419	T2634	T2633	cc	and,node
R142	T360	T359	compound	Otx2,expression
R1420	T2635	T2636	amod	anterior,endoderm
R1421	T2636	T2633	conj	endoderm,node
R1422	T2637	T2636	amod	definitive,endoderm
R1423	T2638	T2639	punct	(,21
R1424	T2639	T2633	parataxis	21,node
R1425	T2640	T2641	nmod	Fig.,I
R1426	T2641	T2639	dep	I,21
R1427	T2642	T2641	nmod	1G,I
R1428	T2643	T2641	punct	",",I
R1429	T2644	T2639	punct	[,21
R143	T361	T353	cc	and,signify
R1430	T2645	T2639	punct	],21
R1431	T2646	T2639	punct	),21
R1432	T2647	T2622	punct	.,confined
R1433	T2649	T2650	compound	Mutant,embryos
R1434	T2650	T2651	nsubj	embryos,exhibit
R1435	T2652	T2653	npadvmod	Hnf3β,reactive
R1436	T2653	T2655	amod	reactive,endoderm
R1437	T2654	T2653	punct	-,reactive
R1438	T2655	T2651	dobj	endoderm,exhibit
R1439	T2656	T2655	amod	definitive,endoderm
R144	T362	T363	nsubj	lack,denote
R1440	T2657	T2651	prep	over,exhibit
R1441	T2658	T2659	det	the,region
R1442	T2659	T2657	pobj	region,over
R1443	T2660	T2659	amod	disorganized,region
R1444	T2661	T2662	amod	anterior,streak
R1445	T2662	T2659	compound	streak,region
R1446	T2663	T2657	cc	and,over
R1447	T2664	T2657	conj	proceeding,over
R1448	T2665	T2664	advmod	rostrally,proceeding
R1449	T2666	T2664	prep	around,proceeding
R145	T363	T353	conj	denote,signify
R1450	T2667	T2668	det	the,tip
R1451	T2668	T2666	pobj	tip,around
R1452	T2669	T2668	amod	distal,tip
R1453	T2670	T2671	punct	(,J
R1454	T2671	T2651	dep	J,exhibit
R1455	T2672	T2671	nmod	Fig.,J
R1456	T2673	T2671	nummod	1H,J
R1457	T2674	T2671	punct	",",J
R1458	T2675	T2671	punct	),J
R1459	T2676	T2651	punct	.,exhibit
R146	T364	T362	prep	of,lack
R1460	T2678	T2679	prep	In,exhibits
R1461	T2680	T2681	preconj	both,normal
R1462	T2681	T2682	amod	normal,embryos
R1463	T2682	T2678	pobj	embryos,In
R1464	T2683	T2681	cc	and,normal
R1465	T2684	T2681	conj	mutant,normal
R1466	T2685	T2679	punct	", ",exhibits
R1467	T2686	T2687	det	the,AVE
R1468	T2687	T2679	nsubj	AVE,exhibits
R1469	T2688	T2689	amod	little,expression
R147	T365	T364	pobj	headfolds,of
R1470	T2689	T2679	dobj	expression,exhibits
R1471	T2690	T2689	compound	Hnf3β,expression
R1472	T2691	T2679	punct	.,exhibits
R1473	T2693	T2694	advmod	Therefore,affect
R1474	T2695	T2694	punct	", ",affect
R1475	T2696	T2697	compound	huntingtin,deficiency
R1476	T2697	T2694	nsubj	deficiency,affect
R1477	T2698	T2694	aux	does,affect
R1478	T2699	T2694	neg	not,affect
R1479	T2700	T2694	advmod	greatly,affect
R148	T366	T367	det	a,failure
R1480	T2701	T2702	compound	Hnf3β,regulation
R1481	T2702	T2694	dobj	regulation,affect
R1482	T2703	T2702	cc	or,regulation
R1483	T2704	T2705	det	the,reorganization
R1484	T2705	T2702	conj	reorganization,regulation
R1485	T2706	T2705	prep	of,reorganization
R1486	T2707	T2708	det	the,endoderm
R1487	T2708	T2706	pobj	endoderm,of
R1488	T2709	T2708	amod	visceral,endoderm
R1489	T2710	T2694	punct	.,affect
R149	T367	T363	dobj	failure,denote
R1490	T2712	T2713	det	The,lack
R1491	T2713	T2714	nsubj	lack,suggest
R1492	T2715	T2713	prep	of,lack
R1493	T2716	T2717	det	a,node
R1494	T2717	T2715	pobj	node,of
R1495	T2718	T2717	amod	morphological,node
R1496	T2719	T2713	cc	and,lack
R1497	T2720	T2713	conj	presence,lack
R1498	T2721	T2720	prep	of,presence
R1499	T2722	T2723	det	a,streak
R15	T218	T217	compound	streak,formation
R150	T368	T367	prep	of,failure
R1500	T2723	T2721	pobj	streak,of
R1501	T2724	T2723	amod	shortened,streak
R1502	T2725	T2713	punct	", ",lack
R1503	T2726	T2727	advmod	together,with
R1504	T2727	T2713	prep	with,lack
R1505	T2728	T2729	amod	reduced,neuroectoderm
R1506	T2729	T2727	pobj	neuroectoderm,with
R1507	T2730	T2729	cc	and,neuroectoderm
R1508	T2731	T2729	conj	lack,neuroectoderm
R1509	T2732	T2731	prep	of,lack
R151	T369	T370	compound	head,development
R1510	T2733	T2732	pobj	headfolds,of
R1511	T2734	T2714	punct	", ",suggest
R1512	T2735	T2736	mark	that,impaired
R1513	T2736	T2714	ccomp	impaired,suggest
R1514	T2737	T2738	amod	anterior,streak
R1515	T2738	T2739	compound	streak,formation
R1516	T2739	T2736	nsubjpass	formation,impaired
R1517	T2740	T2736	aux	may,impaired
R1518	T2741	T2736	auxpass	be,impaired
R1519	T2742	T2736	prep	in,impaired
R152	T370	T368	pobj	development,of
R1520	T2743	T2744	npadvmod	huntingtin,deficient
R1521	T2744	T2745	amod	deficient,embryos
R1522	T2745	T2742	pobj	embryos,in
R1523	T2746	T2714	punct	.,suggest
R1524	T2748	T2749	aux	To,investigate
R1525	T2749	T2750	advcl	investigate,examined
R1526	T2751	T2752	det	this,possibility
R1527	T2752	T2749	dobj	possibility,investigate
R1528	T2753	T2750	punct	", ",examined
R1529	T2754	T2750	nsubj	we,examined
R153	T371	T363	punct	.,denote
R1530	T2755	T2756	compound	mesoderm,formation
R1531	T2756	T2750	dobj	formation,examined
R1532	T2757	T2750	prep	in,examined
R1533	T2758	T2759	compound	mutant,embryos
R1534	T2759	T2757	pobj	embryos,in
R1535	T2760	T2750	punct	.,examined
R1536	T2762	T2763	nsubjpass	Mesoderm,specified
R1537	T2764	T2763	auxpass	is,specified
R1538	T2765	T2763	prep	in,specified
R1539	T2766	T2767	det	the,embryos
R154	T373	T374	prep	In,restricted
R1540	T2767	T2765	pobj	embryos,in
R1541	T2768	T2767	compound	mutant,embryos
R1542	T2769	T2763	punct	", ",specified
R1543	T2770	T2771	mark	as,marked
R1544	T2771	T2763	advcl	marked,specified
R1545	T2772	T2771	agent	by,marked
R1546	T2773	T2774	det	the,expression
R1547	T2774	T2772	pobj	expression,by
R1548	T2775	T2774	prep	of,expression
R1549	T2776	T2775	pobj	T,of
R155	T375	T373	pobj	addition,In
R1550	T2777	T2776	punct	(,T
R1551	T2778	T2776	appos	Brachyury,T
R1552	T2779	T2776	punct	),T
R1553	T2780	T2776	cc	and,T
R1554	T2781	T2776	conj	Evx1,T
R1555	T2782	T2783	punct	(,Fig.
R1556	T2783	T2771	parataxis	Fig.,marked
R1557	T2784	T2783	nummod	2A,Fig.
R1558	T2785	T2786	punct	–,F
R1559	T2786	T2783	prep	F,Fig.
R156	T376	T374	punct	", ",restricted
R1560	T2787	T2783	punct	),Fig.
R1561	T2788	T2763	punct	.,specified
R1562	T2790	T2791	advmod	However,reveals
R1563	T2792	T2791	punct	", ",reveals
R1564	T2793	T2794	amod	close,inspection
R1565	T2794	T2791	nsubj	inspection,reveals
R1566	T2795	T2794	prep	of,inspection
R1567	T2796	T2797	det	the,data
R1568	T2797	T2795	pobj	data,of
R1569	T2798	T2799	amod	abnormal,patterning
R157	T377	T374	nsubjpass	genes,restricted
R1570	T2799	T2791	dobj	patterning,reveals
R1571	T2800	T2791	prep	within,reveals
R1572	T2801	T2802	det	this,tissue
R1573	T2802	T2800	pobj	tissue,within
R1574	T2803	T2802	cc	and,tissue
R1575	T2804	T2805	poss	its,derivatives
R1576	T2805	T2802	conj	derivatives,tissue
R1577	T2806	T2791	punct	.,reveals
R1578	T2807	T2808	nsubjpass	T,detected
R1579	T2810	T2807	punct	", ",T
R158	T378	T379	advmod	initially,expressed
R1580	T2811	T2812	advmod	normally,expressed
R1581	T2812	T2807	acl	expressed,T
R1582	T2813	T2812	prep	in,expressed
R1583	T2814	T2815	det	the,streak
R1584	T2815	T2813	pobj	streak,in
R1585	T2816	T2815	amod	primitive,streak
R1586	T2817	T2815	punct	", ",streak
R1587	T2818	T2815	conj	node,streak
R1588	T2819	T2818	cc	and,node
R1589	T2820	T2821	amod	axial,process
R159	T379	T377	acl	expressed,genes
R1590	T2821	T2818	conj	process,node
R1591	T2822	T2821	compound	head,process
R1592	T2823	T2821	punct	/,process
R1593	T2824	T2825	compound	notochord,mesoderm
R1594	T2825	T2821	appos	mesoderm,process
R1595	T2826	T2827	punct	[,22
R1596	T2827	T2815	parataxis	22,streak
R1597	T2828	T2827	punct	],22
R1598	T2829	T2808	punct	", ",detected
R1599	T2830	T2808	auxpass	is,detected
R16	T219	T217	cc	and,formation
R160	T380	T379	advmod	broadly,expressed
R1600	T2831	T2808	prep	in,detected
R1601	T2832	T2833	det	the,streak
R1602	T2833	T2831	pobj	streak,in
R1603	T2834	T2833	amod	shortened,streak
R1604	T2835	T2833	cc	and,streak
R1605	T2836	T2837	amod	axial,mesoderm
R1606	T2837	T2833	conj	mesoderm,streak
R1607	T2838	T2808	prep	in,detected
R1608	T2839	T2840	nmod	Hdhex4,embryos
R1609	T2840	T2838	pobj	embryos,in
R161	T381	T374	auxpass	are,restricted
R1610	T2841	T2839	punct	/,Hdhex4
R1611	T2842	T2839	nummod	5,Hdhex4
R1612	T2843	T2839	punct	/,Hdhex4
R1613	T2844	T2839	appos	Hdhex4,Hdhex4
R1614	T2845	T2844	punct	/,Hdhex4
R1615	T2846	T2844	nummod	5,Hdhex4
R1616	T2847	T2808	punct	", ",detected
R1617	T2848	T2808	advcl	extending,detected
R1618	T2849	T2848	advmod	rostrally,extending
R1619	T2850	T2848	prep	from,extending
R162	T382	T374	neg	not,restricted
R1620	T2851	T2852	det	a,region
R1621	T2852	T2850	pobj	region,from
R1622	T2853	T2852	prep	of,region
R1623	T2854	T2855	advmod	weakly,positive
R1624	T2855	T2856	amod	positive,cells
R1625	T2856	T2853	pobj	cells,of
R1626	T2857	T2858	punct	(,B
R1627	T2964	T2962	nummod	2E,F
R1628	T2965	T2962	punct	",",F
R1629	T2966	T2962	punct	),F
R163	T383	T374	advmod	properly,restricted
R1630	T2967	T2920	punct	.,expressed
R1631	T2969	T2970	amod	Extraembryonic,mesoderm
R1632	T2970	T2971	nsubj	mesoderm,express
R1633	T2972	T2970	punct	", ",mesoderm
R1634	T2973	T2970	acl	derived,mesoderm
R1635	T2858	T2848	parataxis	B,extending
R1636	T2974	T2973	prep	from,derived
R1637	T2859	T2858	dep	Fig.,B
R1638	T2860	T2859	nummod	2A,Fig.
R1639	T2975	T2976	det	the,streak
R164	T384	T374	prep	to,restricted
R1640	T2861	T2858	punct	",",B
R1641	T2862	T2858	punct	),B
R1642	T2976	T2974	pobj	streak,from
R1643	T2863	T2808	punct	.,detected
R1644	T2977	T2976	amod	proximal,streak
R1645	T2865	T2866	compound	T,expression
R1646	T2866	T2867	nsubj	expression,appears
R1647	T2978	T2971	punct	", ",express
R1648	T2868	T2867	oprd	weaker,appears
R1649	T2869	T2867	punct	", ",appears
R165	T385	T386	det	the,posterior
R1650	T2979	T2971	aux	does,express
R1651	T2870	T2867	advmod	however,appears
R1652	T2871	T2867	punct	", ",appears
R1653	T2872	T2867	prep	in,appears
R1654	T2980	T2971	neg	not,express
R1655	T2873	T2874	det	the,streak
R1656	T2874	T2872	pobj	streak,in
R1657	T2875	T2874	amod	anterior,streak
R1658	T2981	T2971	advmod	normally,express
R1659	T2876	T2867	punct	", ",appears
R166	T386	T384	pobj	posterior,to
R1660	T2877	T2867	advcl	corresponding,appears
R1661	T2878	T2877	prep	to,corresponding
R1662	T2982	T2971	dobj	T,express
R1663	T2879	T2878	pobj	cells,to
R1664	T2880	T2881	dep	that,give
R1665	T2881	T2879	relcl	give,cells
R1666	T2983	T2982	cc	or,T
R1667	T2882	T2881	aux	will,give
R1668	T2883	T2881	dobj	rise,give
R1669	T2884	T2881	prep	to,give
R167	T387	T374	punct	", ",restricted
R1670	T2984	T2982	conj	Evx1,T
R1671	T2885	T2886	amod	axial,mesoderm
R1672	T2886	T2884	pobj	mesoderm,to
R1673	T2985	T2971	prep	in,express
R1674	T2887	T2888	punct	(,Fig.
R1675	T2888	T2881	parataxis	Fig.,give
R1676	T2889	T2888	nummod	2D,Fig.
R1677	T2986	T2987	amod	wild,type
R1678	T2890	T2888	punct	),Fig.
R1679	T2891	T2867	punct	.,appears
R168	T388	T389	mark	as,evidenced
R1680	T2893	T2894	nsubjpass	T,expressed
R1681	T2895	T2894	auxpass	is,expressed
R1682	T2987	T2989	compound	type,embryos
R1683	T2896	T2894	advmod	also,expressed
R1684	T2897	T2894	advmod	ectopically,expressed
R1685	T2898	T2894	prep	in,expressed
R1686	T2899	T2900	nmod	mutant,mesoderm
R1687	T2988	T2987	punct	-,type
R1688	T2900	T2898	pobj	mesoderm,in
R1689	T2901	T2900	amod	extraembryonic,mesoderm
R169	T389	T374	advcl	evidenced,restricted
R1690	T2902	T2894	prep	at,expressed
R1691	T2989	T2985	pobj	embryos,in
R1692	T2903	T2904	det	the,junction
R1693	T2904	T2902	pobj	junction,at
R1694	T2905	T2904	amod	anterior,junction
R1695	T2990	T2991	punct	[,22
R1696	T2906	T2904	amod	embryonic,junction
R1697	T2907	T2902	cc	and,at
R1698	T2908	T2902	conj	along,at
R1699	T2909	T2910	det	the,chorion
R17	T220	T221	amod	early,patterning
R170	T390	T389	agent	by,evidenced
R1700	T2991	T2971	parataxis	22,express
R1701	T2910	T2908	pobj	chorion,along
R1702	T2911	T2912	punct	(,D
R1703	T2992	T2991	punct	],22
R1704	T2912	T2894	parataxis	D,expressed
R1705	T2913	T2912	nmod	Fig.,D
R1706	T2914	T2912	nummod	2B,D
R1707	T2993	T2971	punct	.,express
R1708	T2915	T2912	punct	",",D
R1709	T2916	T2912	punct	),D
R171	T391	T392	det	the,expression
R1710	T2917	T2894	punct	.,expressed
R1711	T2995	T2996	advmod	Therefore,suggest
R1712	T2919	T2920	advmod	Similarly,expressed
R1713	T2921	T2920	punct	", ",expressed
R1714	T2997	T2996	punct	", ",suggest
R1715	T2922	T2920	nsubjpass	Evx1,expressed
R1716	T2923	T2922	punct	", ",Evx1
R1717	T2924	T2925	advmod	normally,expressed
R1718	T2925	T2922	acl	expressed,Evx1
R1719	T2926	T2925	prep	in,expressed
R172	T392	T390	pobj	expression,by
R1720	T2998	T2999	det	the,expression
R1721	T2927	T2928	amod	primitive,mesoderm
R1722	T2928	T2926	pobj	mesoderm,in
R1723	T2929	T2928	compound	streak,mesoderm
R1724	T2999	T2996	nsubj	expression,suggest
R1725	T2930	T2925	prep	at,expressed
R1726	T2931	T2930	pobj	E7.5,at
R1727	T2932	T2933	mark	with,levels
R1728	T3000	T2999	amod	inappropriate,expression
R1729	T2933	T2925	advcl	levels,expressed
R173	T393	T392	amod	ectopic,expression
R1730	T2934	T2933	amod	highest,levels
R1731	T2935	T2933	prep	in,levels
R1732	T2936	T2937	amod	proximal,cells
R1733	T2937	T2935	pobj	cells,in
R1734	T3001	T2999	prep	of,expression
R1735	T2938	T2939	punct	[,23
R1736	T2939	T2933	parataxis	23,levels
R1737	T3002	T3001	pobj	T,of
R1738	T2940	T2939	punct	],23
R1739	T3003	T3002	cc	and,T
R174	T394	T392	prep	of,expression
R1740	T2941	T2920	punct	", ",expressed
R1741	T3004	T3002	conj	Evx1,T
R1742	T2942	T2920	auxpass	is,expressed
R1743	T3005	T2999	punct	", ",expression
R1744	T2943	T2920	prep	in,expressed
R1745	T2944	T2945	det	the,streak
R1746	T2945	T2943	pobj	streak,in
R1747	T3006	T3007	det	the,streak
R1748	T2946	T2945	amod	proximal,streak
R1749	T2947	T2945	amod	shortened,streak
R175	T395	T394	pobj	Nodal,of
R1750	T3007	T2999	conj	streak,expression
R1751	T2948	T2920	cc	but,expressed
R1752	T2949	T2950	auxpass	is,expressed
R1753	T2950	T2920	conj	expressed,expressed
R1754	T3008	T3007	amod	shortened,streak
R1755	T2951	T2950	advmod	also,expressed
R1756	T2952	T2950	advmod	aberrantly,expressed
R1757	T2953	T2950	prep	throughout,expressed
R1758	T3009	T3007	amod	primitive,streak
R1759	T2954	T2955	det	the,mesoderm
R176	T396	T395	punct	", ",Nodal
R1760	T2955	T2953	pobj	mesoderm,throughout
R1761	T2956	T2955	amod	extraembryonic,mesoderm
R1762	T2957	T2955	punct	", ",mesoderm
R1763	T3010	T3007	punct	", ",streak
R1764	T2958	T2955	conj	allantois,mesoderm
R1765	T2959	T2958	cc	and,allantois
R1766	T2960	T2958	conj	chorion,allantois
R1767	T3011	T3007	cc	and,streak
R1768	T2961	T2962	punct	(,F
R1769	T2962	T2950	parataxis	F,expressed
R177	T397	T395	conj	Fgf8,Nodal
R1770	T3012	T3013	det	the,absence
R1771	T2963	T2962	nmod	Fig.,F
R1772	T3013	T3007	conj	absence,streak
R1773	T3014	T3013	prep	of,absence
R1774	T3015	T3016	det	a,node
R1775	T3016	T3014	pobj	node,of
R1776	T3017	T3016	amod	morphological,node
R1777	T3018	T2999	punct	", ",expression
R1778	T3019	T2999	appos	all,expression
R1779	T3020	T3021	mark	that,is
R178	T398	T397	cc	and,Fgf8
R1780	T3021	T2996	ccomp	is,suggest
R1781	T3022	T3023	det	the,streak
R1782	T3033	T3034	det	The,streak
R1783	T3034	T3036	nsubj	streak,generates
R1784	T3035	T3034	amod	anterior,streak
R1785	T3037	T3038	amod	paraxial,mesoderm
R1786	T3023	T3021	nsubj	streak,is
R1787	T3038	T3036	dobj	mesoderm,generates
R1788	T3024	T3023	amod	anterior,streak
R1789	T3039	T3036	punct	.,generates
R179	T399	T397	conj	Gsc,Fgf8
R1790	T3025	T3023	amod	primitive,streak
R1791	T3041	T3042	advmod	Therefore,examined
R1792	T3043	T3042	nsubj	we,examined
R1793	T3026	T3021	acomp	deficient,is
R1794	T3044	T3045	amod	paraxial,mesoderm
R1795	T3027	T3021	prep	in,is
R1796	T3045	T3046	compound	mesoderm,formation
R1797	T3046	T3042	dobj	formation,examined
R1798	T3047	T3046	prep	in,formation
R1799	T3048	T3049	amod	wild,type
R18	T221	T217	conj	patterning,formation
R180	T400	T392	prep	in,expression
R1800	T3028	T3029	det	the,embryos
R1801	T3049	T3051	nmod	type,embryos
R1802	T3050	T3049	punct	-,type
R1803	T3029	T3027	pobj	embryos,in
R1804	T3051	T3047	pobj	embryos,in
R1805	T3052	T3049	cc	and,type
R1806	T3053	T3049	conj	mutant,type
R1807	T3054	T3042	punct	", ",examined
R1808	T3030	T3029	compound	mutant,embryos
R1809	T3055	T3042	advcl	revealing,examined
R181	T401	T402	det	the,epiblast
R1810	T3056	T3055	dobj	deficits,revealing
R1811	T3057	T3056	prep	in,deficits
R1812	T3031	T2996	punct	.,suggest
R1813	T3058	T3059	compound	mesoderm,patterning
R1814	T3059	T3057	pobj	patterning,in
R1815	T3060	T3042	punct	.,examined
R1816	T3084	T3085	npadvmod	huntingtin,deficient
R1817	T3062	T3063	advcl	Starting,expressed
R1818	T3064	T3062	prep	at,Starting
R1819	T3065	T3064	pobj	E.7.5,at
R182	T402	T400	pobj	epiblast,in
R1820	T3066	T3063	punct	", ",expressed
R1821	T3067	T3063	nsubjpass	Wnt3A,expressed
R1822	T3068	T3063	auxpass	is,expressed
R1823	T3069	T3063	prep	in,expressed
R1824	T3085	T3086	amod	deficient,mutants
R1825	T3070	T3071	det	the,streak
R1826	T3071	T3069	pobj	streak,in
R1827	T3072	T3071	amod	primitive,streak
R1828	T3086	T3082	pobj	mutants,In
R1829	T3073	T3063	prep	in,expressed
R183	T403	T402	cc	and,epiblast
R1830	T3087	T3083	punct	", ",induced
R1831	T3074	T3073	pobj	cells,in
R1832	T3075	T3074	acl	fated,cells
R1833	T3088	T3083	nsubjpass	Wnt3a,induced
R1834	T3076	T3077	aux	to,become
R1835	T3077	T3075	xcomp	become,fated
R1836	T3078	T3079	amod	paraxial,mesoderm
R1837	T3079	T3077	attr	mesoderm,become
R1838	T3080	T3063	punct	.,expressed
R1839	T3082	T3083	prep	In,induced
R184	T404	T405	nmod	T,Brachyury
R1840	T3089	T3083	auxpass	is,induced
R1841	T3090	T3083	prep	in,induced
R1842	T3091	T3092	det	the,streak
R1843	T3092	T3090	pobj	streak,in
R1844	T3093	T3092	amod	proximal,streak
R1845	T3190	T3188	pobj	region,in
R1846	T3094	T3095	punct	(,H
R1847	T3191	T3190	amod	distal,region
R1848	T3192	T3190	compound	streak,region
R1849	T3095	T3092	parataxis	H,streak
R185	T405	T402	conj	Brachyury,epiblast
R1850	T3193	T3188	cc	and,in
R1851	T3194	T3188	conj	in,in
R1852	T3096	T3095	nmod	Fig.,H
R1853	T3195	T3196	advmod	only,swath
R1854	T3196	T3194	pobj	swath,in
R1855	T3097	T3095	nummod	2G,H
R1856	T3197	T3196	det	a,swath
R1857	T3198	T3196	amod	narrow,swath
R1858	T3199	T3196	prep	of,swath
R1859	T3098	T3095	punct	",",H
R186	T406	T405	punct	(,Brachyury
R1860	T3200	T3199	pobj	cells,of
R1861	T3201	T3196	acl	located,swath
R1862	T3099	T3095	punct	),H
R1863	T3202	T3201	advmod	laterally,located
R1864	T3203	T3204	det	the,paucity
R1865	T3204	T3176	dobj	paucity,confirms
R1866	T3100	T3083	punct	", ",induced
R1867	T3205	T3204	prep	of,paucity
R1868	T3206	T3207	amod	paraxial,mesoderm
R1869	T3101	T3083	advcl	confirming,induced
R187	T407	T405	punct	),Brachyury
R1870	T3207	T3205	pobj	mesoderm,of
R1871	T3208	T3209	punct	(,24
R1872	T3209	T3176	parataxis	24,confirms
R1873	T3102	T3103	npadvmod	stage,appropriate
R1874	T3210	T3211	nmod	Fig.,L
R1875	T3211	T3209	dep	L,24
R1876	T3212	T3211	nummod	2K,L
R1877	T3103	T3104	amod	appropriate,development
R1878	T3213	T3211	punct	",",L
R1879	T3214	T3209	punct	", ",24
R188	T408	T405	cc	and,Brachyury
R1880	T3215	T3209	punct	[,24
R1881	T3104	T3101	dobj	development,confirming
R1882	T3216	T3209	punct	],24
R1883	T3217	T3209	punct	),24
R1884	T3218	T3176	punct	.,confirms
R1885	T3105	T3104	amod	posterior,development
R1886	T3220	T3221	det	These,results
R1887	T3221	T3222	nsubj	results,suggest
R1888	T3223	T3222	advmod	strongly,suggest
R1889	T3224	T3225	mark	that,is
R189	T409	T405	conj	Evx1,Brachyury
R1890	T3225	T3222	ccomp	is,suggest
R1891	T3226	T3227	amod	anterior,streak
R1892	T3106	T3083	punct	", ",induced
R1893	T3227	T3229	compound	streak,formation
R1894	T3228	T3227	amod	primitive,streak
R1895	T3229	T3225	nsubj	formation,is
R1896	T3107	T3083	prep	in,induced
R1897	T3230	T3225	acomp	impaired,is
R1898	T3231	T3225	punct	", ",is
R1899	T3108	T3107	pobj	contrast,in
R19	T222	T221	prep	of,patterning
R190	T410	T392	prep	in,expression
R1900	T3232	T3225	advcl	resulting,is
R1901	T3109	T3108	prep	to,contrast
R1902	T3233	T3232	prep	in,resulting
R1903	T3234	T3235	amod	reduced,formation
R1904	T3110	T3111	det	the,absence
R1905	T3235	T3233	pobj	formation,in
R1906	T3236	T3237	amod	axial,mesoderm
R1907	T3237	T3235	compound	mesoderm,formation
R1908	T3238	T3236	cc	and,axial
R1909	T3111	T3109	pobj	absence,to
R191	T411	T412	amod	proximal,derivatives
R1910	T3239	T3236	conj	paraxial,axial
R1911	T3240	T3235	cc	and,formation
R1912	T3112	T3111	prep	of,absence
R1913	T3241	T3242	amod	impaired,development
R1914	T3242	T3235	conj	development,formation
R1915	T3243	T3242	amod	neural,development
R1916	T3113	T3114	amod	anterior,folds
R1917	T3244	T3222	punct	.,suggest
R1918	T3114	T3112	pobj	folds,of
R1919	T3115	T3114	compound	head,folds
R192	T412	T410	pobj	derivatives,in
R1920	T3116	T3083	punct	.,induced
R1921	T3118	T3119	advmod	However,reduced
R1922	T3120	T3119	punct	", ",reduced
R1923	T3121	T3119	nsubjpass	expression,reduced
R1924	T3122	T3121	prep	of,expression
R1925	T3123	T3122	pobj	Wnt3a,of
R1926	T3124	T3119	auxpass	is,reduced
R1927	T3125	T3119	advmod	noticeably,reduced
R1928	T3126	T3119	prep	in,reduced
R1929	T3127	T3128	nmod	Hdhex4,embryos
R193	T413	T412	compound	mesoderm,derivatives
R1930	T3128	T3126	pobj	embryos,in
R1931	T3129	T3127	punct	/,Hdhex4
R1932	T3130	T3127	nummod	5,Hdhex4
R1933	T3131	T3127	punct	/,Hdhex4
R1934	T3132	T3127	appos	Hdhex4,Hdhex4
R1935	T3133	T3132	punct	/,Hdhex4
R1936	T3134	T3132	nummod	5,Hdhex4
R1937	T3135	T3119	punct	", ",reduced
R1938	T3136	T3119	advcl	suggesting,reduced
R1939	T3137	T3138	det	a,defect
R194	T414	T374	punct	.,restricted
R1940	T3138	T3136	dobj	defect,suggesting
R1941	T3139	T3138	prep	in,defect
R1942	T3140	T3141	amod	paraxial,mesoderm
R1943	T3141	T3142	compound	mesoderm,development
R1944	T3142	T3139	pobj	development,in
R1945	T3143	T3144	punct	(,Fig.
R1946	T3144	T3136	parataxis	Fig.,suggesting
R1947	T3145	T3144	nummod	2H,Fig.
R1948	T3146	T3144	punct	),Fig.
R1949	T3147	T3119	punct	.,reduced
R195	T416	T417	prep	Despite,established
R1950	T3149	T3150	amod	Reduced,expression
R1951	T3150	T3151	nsubj	expression,confirms
R1952	T3152	T3150	prep	of,expression
R1953	T3153	T3152	pobj	Tbx6,of
R1954	T3154	T3150	prep	in,expression
R1955	T3155	T3156	det	the,mesoderm
R1956	T3156	T3154	pobj	mesoderm,in
R1957	T3157	T3156	amod	lateral,mesoderm
R1958	T3158	T3157	prep	to,lateral
R1959	T3159	T3160	det	the,streak
R196	T418	T419	amod	impaired,restriction
R1960	T3160	T3158	pobj	streak,to
R1961	T3161	T3160	amod	primitive,streak
R1962	T3162	T3156	prep	in,mesoderm
R1963	T3163	T3164	compound	mutant,embryos
R1964	T3164	T3162	pobj	embryos,in
R1965	T3165	T3166	det	this,interpretation
R1966	T3166	T3151	dobj	interpretation,confirms
R1967	T3167	T3168	punct	(,J
R1968	T3168	T3166	parataxis	J,interpretation
R1969	T3169	T3168	nmod	Fig.,J
R197	T419	T416	pobj	restriction,Despite
R1970	T3170	T3168	nummod	2I,J
R1971	T3171	T3168	punct	",",J
R1972	T3172	T3168	punct	),J
R1973	T3173	T3151	punct	.,confirms
R1974	T3175	T3176	advmod	Furthermore,confirms
R1975	T3177	T3176	punct	", ",confirms
R1976	T3178	T3176	prep	in,confirms
R1977	T3179	T3180	compound	mutant,embryos
R1978	T3180	T3178	pobj	embryos,in
R1979	T3181	T3176	prep	at,confirms
R198	T420	T419	amod	posterior,restriction
R1980	T3182	T3181	pobj	E7.5,at
R1981	T3183	T3176	punct	", ",confirms
R1982	T3184	T3185	det	the,expression
R1983	T3185	T3176	nsubj	expression,confirms
R1984	T3186	T3185	prep	of,expression
R1985	T3187	T3186	pobj	Dll1,of
R1986	T3188	T3185	prep	in,expression
R1987	T3189	T3190	det	the,region
R199	T421	T419	cc	and,restriction
R1994	T3609	T3610	amod	Impaired,restriction
R1995	T3611	T3610	amod	regional,restriction
R1996	T3612	T3610	prep	of,restriction
R1997	T3613	T3614	compound	growth,factor
R1998	T3614	T3615	compound	factor,expression
R1999	T3615	T3612	pobj	expression,of
R20	T223	T224	det	the,embryo
R200	T422	T423	amod	anterior,streak
R2000	T3616	T3610	prep	in,restriction
R2001	T3617	T3618	det	the,absence
R2002	T3618	T3616	pobj	absence,in
R2003	T3619	T3618	prep	of,absence
R2004	T3620	T3619	pobj	huntingtin,of
R2005	T3622	T3623	aux	To,elucidate
R2006	T3623	T3624	advcl	elucidate,analyzed
R2007	T3625	T3626	det	the,deficits
R2008	T3626	T3623	dobj	deficits,elucidate
R2009	T3627	T3626	amod	apparent,deficits
R201	T423	T424	compound	streak,deficits
R2010	T3628	T3626	compound	patterning,deficits
R2011	T3629	T3624	punct	", ",analyzed
R2012	T3630	T3624	nsubj	we,analyzed
R2013	T3631	T3624	advmod	next,analyzed
R2014	T3632	T3633	compound	signaling,molecules
R2015	T3633	T3624	dobj	molecules,analyzed
R2016	T3634	T3635	dep	that,required
R2017	T3635	T3633	relcl	required,molecules
R2018	T3636	T3635	auxpass	are,required
R2019	T3637	T3635	prep	for,required
R202	T424	T419	conj	deficits,restriction
R2020	T3638	T3639	amod	early,patterning
R2021	T3639	T3637	pobj	patterning,for
R2022	T3640	T3624	punct	.,analyzed
R2023	T3642	T3643	nsubjpass	Nodal,required
R2024	T3644	T3642	punct	", ",Nodal
R2025	T3645	T3646	det	a,member
R2026	T3646	T3642	appos	member,Nodal
R2027	T3647	T3646	prep	of,member
R2028	T3648	T3649	det	the,family
R2029	T3649	T3647	pobj	family,of
R203	T425	T417	punct	", ",established
R2030	T3650	T3649	compound	Tgfβ,family
R2031	T3651	T3649	prep	of,family
R2032	T3652	T3653	amod	secreted,molecules
R2033	T3653	T3651	pobj	molecules,of
R2034	T3654	T3643	auxpass	is,required
R2035	T3655	T3643	prep	for,required
R2036	T3656	T3657	det	the,formation
R2037	T3657	T3655	pobj	formation,for
R2038	T3658	T3657	cc	and,formation
R2039	T3659	T3657	conj	maintenance,formation
R204	T426	T427	amod	overall,polarity
R2040	T3660	T3657	prep	of,formation
R2041	T3661	T3662	det	the,streak
R2042	T3662	T3660	pobj	streak,of
R2043	T3663	T3662	amod	primitive,streak
R2044	T3664	T3657	cc	and,formation
R2045	T3665	T3657	conj	induction,formation
R2046	T3666	T3665	prep	of,induction
R2047	T3667	T3668	det	the,AVE
R2048	T3668	T3666	pobj	AVE,of
R2049	T3669	T3670	punct	[,25
R205	T427	T417	nsubjpass	polarity,established
R2050	T3670	T3643	parataxis	25,required
R2051	T3671	T3672	punct	-,27
R2052	T3672	T3670	prep	27,25
R2053	T3673	T3670	punct	],25
R2054	T3674	T3643	punct	.,required
R2055	T3676	T3677	nsubjpass	Nodal,expressed
R2056	T3678	T3677	auxpass	is,expressed
R2057	T3679	T3677	advmod	normally,expressed
R2058	T3680	T3677	prep	throughout,expressed
R2059	T3681	T3682	det	the,epiblast
R206	T428	T429	amod	anterior,posterior
R2060	T3682	T3680	pobj	epiblast,throughout
R2061	T3683	T3682	cc	and,epiblast
R2062	T3684	T3685	amod	overlying,endoderm
R2063	T3685	T3682	conj	endoderm,epiblast
R2064	T3686	T3685	amod	visceral,endoderm
R2065	T3687	T3677	prep	at,expressed
R2066	T3688	T3689	amod	early,stages
R2067	T3689	T3687	pobj	stages,at
R2068	T3690	T3691	nmod	post,implantation
R2069	T3691	T3689	compound	implantation,stages
R207	T429	T427	amod	posterior,polarity
R2070	T3692	T3693	punct	[,28
R2071	T3693	T3677	parataxis	28,expressed
R2072	T3694	T3693	punct	],28
R2073	T3695	T3677	punct	", ",expressed
R2074	T3696	T3677	cc	but,expressed
R2075	T3697	T3698	advmod	later,becomes
R2076	T3698	T3677	conj	becomes,expressed
R2077	T3699	T3698	acomp	restricted,becomes
R2078	T3700	T3699	prep	to,restricted
R2079	T3701	T3702	det	the,posterior
R208	T430	T429	punct	/,posterior
R2080	T3702	T3700	pobj	posterior,to
R2081	T3703	T3702	prep	of,posterior
R2082	T3704	T3705	det	the,embryo
R2083	T3705	T3703	pobj	embryo,of
R2084	T3706	T3702	prep	to,posterior
R2085	T3707	T3708	det	the,site
R2086	T3708	T3706	pobj	site,to
R2087	T3709	T3708	prep	of,site
R2088	T3710	T3711	amod	primitive,streak
R2089	T3711	T3709	pobj	streak,of
R209	T431	T417	auxpass	is,established
R2090	T3712	T3698	prep	with,becomes
R2091	T3713	T3714	amod	asymmetrical,expression
R2092	T3714	T3717	nsubj	expression,marking
R2093	T3715	T3714	amod	visceral,expression
R2094	T3716	T3714	compound	endoderm,expression
R2095	T3717	T3712	pcomp	marking,with
R2096	T3718	T3719	det	the,axis
R2097	T3719	T3717	dobj	axis,marking
R2098	T3720	T3721	amod	left,right
R2099	T3721	T3719	amod	right,axis
R21	T224	T222	pobj	embryo,of
R210	T432	T417	punct	.,established
R2100	T3722	T3721	punct	-,right
R2101	T3723	T3677	punct	.,expressed
R2102	T3725	T3726	prep	By,restricted
R2103	T3727	T3725	pobj	E7.5,By
R2104	T3728	T3726	punct	", ",restricted
R2105	T3729	T3730	compound	Nodal,expression
R2106	T3730	T3726	nsubjpass	expression,restricted
R2107	T3731	T3726	auxpass	is,restricted
R2108	T3732	T3726	prep	to,restricted
R2109	T3733	T3734	det	the,node
R211	T434	T435	det	A,streak
R2110	T3734	T3732	pobj	node,to
R2111	T3735	T3726	punct	.,restricted
R2112	T3737	T3738	compound	Nodal,expression
R2113	T3738	T3739	nsubjpass	expression,assessed
R2114	T3740	T3739	auxpass	was,assessed
R2115	T3741	T3739	prep	in,assessed
R2116	T3742	T3743	nmod	Hdhex4,embryos
R2117	T3743	T3741	pobj	embryos,in
R2118	T3744	T3742	punct	/,Hdhex4
R2119	T3745	T3742	nummod	5,Hdhex4
R212	T435	T438	nsubj	streak,forms
R2120	T3746	T3742	punct	/,Hdhex4
R2121	T3747	T3742	appos	Hdhex4,Hdhex4
R2122	T3748	T3747	punct	/,Hdhex4
R2123	T3749	T3747	nummod	5,Hdhex4
R2124	T3750	T3743	amod	heterozygous,embryos
R2125	T3751	T3750	prep	for,heterozygous
R2126	T3752	T3753	det	the,allele
R2127	T3753	T3751	pobj	allele,for
R2128	T3754	T3755	compound	Ndl,Z
R2129	T3755	T3753	compound	Z,allele
R213	T436	T435	amod	single,streak
R2130	T3756	T3755	compound	lac,Z
R2131	T3757	T3758	punct	[,29
R2132	T3758	T3739	parataxis	29,assessed
R2133	T3759	T3758	nummod	28,29
R2134	T3760	T3758	punct	",",29
R2135	T3761	T3758	punct	],29
R2136	T3762	T3739	punct	.,assessed
R2137	T3764	T3765	advmod	Notably,alter
R2138	T3766	T3765	punct	", ",alter
R2139	T3767	T3768	amod	heterozygous,loss
R214	T437	T435	amod	primitive,streak
R2140	T3768	T3765	nsubj	loss,alter
R2141	T3769	T3768	prep	of,loss
R2142	T3770	T3769	pobj	nodal,of
R2143	T3771	T3765	aux	does,alter
R2144	T3772	T3765	neg	not,alter
R2145	T3773	T3774	det	the,phenotype
R2146	T3774	T3765	dobj	phenotype,alter
R2147	T3775	T3774	nmod	Hdhex4,phenotype
R2148	T3776	T3775	punct	/,Hdhex4
R2149	T3777	T3775	nummod	5,Hdhex4
R215	T439	T438	cc	and,forms
R2150	T3778	T3775	punct	/,Hdhex4
R2151	T3779	T3775	appos	Hdhex4,Hdhex4
R2152	T3780	T3779	punct	/,Hdhex4
R2153	T3781	T3779	nummod	5,Hdhex4
R2154	T3782	T3765	punct	", ",alter
R2155	T3783	T3784	mark	as,determined
R2156	T3784	T3765	advcl	determined,alter
R2157	T3785	T3784	prep	by,determined
R2158	T3786	T3785	pobj	morphology,by
R2159	T3787	T3786	prep	of,morphology
R216	T440	T441	compound	marker,expression
R2160	T3788	T3789	nmod	Hdhex4,embryos
R2161	T3789	T3787	pobj	embryos,of
R2162	T3790	T3788	punct	/,Hdhex4
R2163	T3791	T3788	nummod	5,Hdhex4
R2164	T3792	T3788	punct	/,Hdhex4
R2165	T3793	T3788	appos	Hdhex4,Hdhex4
R2166	T3794	T3793	punct	/,Hdhex4
R2167	T3795	T3793	nummod	5,Hdhex4
R2168	T3796	T3788	punct	:,Hdhex4
R2169	T3797	T3788	appos	Ndllacz,Hdhex4
R217	T441	T442	nsubj	expression,shows
R2170	T3798	T3797	punct	/,Ndllacz
R2171	T3799	T3797	appos	Ndl,Ndllacz
R2172	T3800	T3799	punct	+,Ndl
R2173	T3801	T3786	prep	compared,morphology
R2174	T3802	T3801	prep	with,compared
R2175	T3803	T3804	nmod	Hdhex4,embryos
R2176	T3804	T3802	pobj	embryos,with
R2177	T3805	T3803	punct	/,Hdhex4
R2178	T3806	T3803	nummod	5,Hdhex4
R2179	T3807	T3803	punct	/,Hdhex4
R218	T442	T438	conj	shows,forms
R2180	T3808	T3803	appos	Hdhex4,Hdhex4
R2181	T3809	T3808	punct	/,Hdhex4
R2182	T3810	T3808	nummod	5,Hdhex4
R2183	T3811	T3812	punct	(,shown
R2184	T3812	T3784	parataxis	shown,determined
R2185	T3813	T3812	nsubj	data,shown
R2186	T3814	T3812	neg	not,shown
R2187	T3815	T3812	punct	),shown
R2188	T3816	T3765	punct	.,alter
R2189	T3818	T3819	prep	In,express
R219	T443	T444	mark	that,specified
R2190	T3820	T3818	pobj	contrast,In
R2191	T3821	T3820	prep	to,contrast
R2192	T3822	T3823	amod	wild,type
R2193	T3823	T3825	compound	type,embryos
R2194	T3824	T3823	punct	-,type
R2195	T3825	T3821	pobj	embryos,to
R2196	T3826	T3825	punct	", ",embryos
R2197	T3827	T3828	dep	which,exhibit
R2198	T3828	T3825	relcl	exhibit,embryos
R2199	T3829	T3830	amod	tight,restriction
R22	T225	T224	compound	mouse,embryo
R220	T444	T442	ccomp	specified,shows
R2200	T3830	T3828	dobj	restriction,exhibit
R2201	T3831	T3830	prep	of,restriction
R2202	T3832	T3833	compound	Nodal,LacZ
R2203	T3833	T3835	compound	LacZ,expression
R2204	T3834	T3833	punct	.,LacZ
R2205	T3835	T3831	pobj	expression,of
R2206	T3836	T3835	prep	to,expression
R2207	T3837	T3838	det	the,node
R2208	T3838	T3836	pobj	node,to
R2209	T3839	T3819	punct	", ",express
R221	T445	T446	det	the,epiblast
R2210	T3840	T3841	nmod	Hdhex4,embryos
R2211	T3841	T3819	nsubj	embryos,express
R2212	T3842	T3840	punct	/,Hdhex4
R2213	T3843	T3840	nummod	5,Hdhex4
R2214	T3844	T3840	punct	/,Hdhex4
R2215	T3845	T3840	appos	Hdhex4,Hdhex4
R2216	T3846	T3845	punct	/,Hdhex4
R2217	T3847	T3845	nummod	5,Hdhex4
R2218	T3848	T3840	punct	:,Hdhex4
R2219	T3849	T3840	appos	Ndllacz,Hdhex4
R222	T446	T444	nsubjpass	epiblast,specified
R2220	T3850	T3849	punct	/,Ndllacz
R2221	T3851	T3849	appos	Ndl,Ndllacz
R2222	T3852	T3851	punct	+,Ndl
R2223	T3853	T3854	compound	Nodal,LacZ
R2224	T3854	T3819	dobj	LacZ,express
R2225	T3855	T3854	punct	.,LacZ
R2226	T3856	T3819	prep	throughout,express
R2227	T3857	T3858	det	the,endoderm
R2228	T3858	T3856	pobj	endoderm,throughout
R2229	T3859	T3858	acl	overlying,endoderm
R223	T447	T446	amod	anterior,epiblast
R2230	T3860	T3861	det	the,epiblast
R2231	T3861	T3859	dobj	epiblast,overlying
R2232	T3862	T3819	punct	", ",express
R2233	T3863	T3864	mark	with,levels
R2234	T3864	T3819	advcl	levels,express
R2235	T3865	T3864	amod	higher,levels
R2236	T3866	T3864	prep	in,levels
R2237	T3867	T3868	det	the,posterior
R2238	T3868	T3866	pobj	posterior,in
R2239	T3869	T3864	prep	in,levels
R224	T448	T446	cc	and,epiblast
R2240	T3870	T3871	det	an,pattern
R2241	T3871	T3869	pobj	pattern,in
R2242	T3872	T3871	amod	asymmetric,pattern
R2243	T3873	T3874	punct	(,3A
R2244	T3874	T3819	parataxis	3A,express
R2245	T3875	T3874	compound	Fig.,3A
R2246	T3876	T3877	punct	–,D
R2247	T3877	T3874	prep	D,3A
R2248	T3878	T3874	punct	),3A
R2249	T3879	T3819	punct	.,express
R225	T449	T450	amod	anterior,endoderm
R2250	T3881	T3882	det	The,lack
R2251	T3882	T3883	nsubj	lack,is
R2252	T3884	T3882	prep	of,lack
R2253	T3885	T3886	amod	tight,restriction
R2254	T3886	T3884	pobj	restriction,of
R2255	T3887	T3886	prep	of,restriction
R2256	T3888	T3889	compound	nodal,signal
R2257	T3889	T3887	pobj	signal,of
R2258	T3890	T3883	acomp	consistent,is
R2259	T3891	T3890	prep	with,consistent
R226	T450	T446	conj	endoderm,epiblast
R2260	T3892	T3893	det	a,failure
R2261	T3893	T3891	pobj	failure,with
R2262	T3894	T3895	aux	to,form
R2263	T3895	T3893	acl	form,failure
R2264	T3896	T3897	det	an,structure
R2265	T3897	T3895	dobj	structure,form
R2266	T3898	T3897	amod	organized,structure
R2267	T3899	T3897	compound	node,structure
R2268	T3900	T3883	punct	.,is
R2269	T3910	T3909	prep	n,g
R227	T451	T450	amod	visceral,endoderm
R2270	T3916	T3914	prep	i,in
R2271	T3917	T3916	pobj	s,i
R2272	T3922	T3921	dobj	o,l
R2273	T3923	T3922	amod	s,o
R2274	T3925	T3920	auxpass	s,e
R2275	T3926	T3920	prep	n,e
R2276	T3937	T3938	compound	g,a
R2277	T3938	T3939	nsubj	a,s
R2278	T3940	T3939	acomp	t,s
R2279	T3941	T3940	prep	r,t
R228	T452	T450	punct	(,endoderm
R2280	T3942	T3941	pobj	ulation,r
R2281	T3943	T3939	prep	in,s
R2282	T3944	T3945	det	the,embryo
R2283	T3945	T3943	pobj	embryo,in
R2284	T3946	T3945	compound	mouse,embryo
R2285	T3947	T3939	punct	.,s
R2286	T3949	T3950	nsubjpass	Fgf8,required
R2287	T3951	T3950	auxpass	is,required
R2288	T3952	T3950	prep	for,required
R2289	T3953	T3954	compound	cell,migration
R229	T453	T450	appos	AVE,endoderm
R2290	T3954	T3952	pobj	migration,for
R2291	T3955	T3954	advmod	away,migration
R2292	T3956	T3955	prep	from,away
R2293	T3957	T3958	det	the,streak
R2294	T3958	T3956	pobj	streak,from
R2295	T3959	T3958	amod	primitive,streak
R2296	T3960	T3961	punct	[,30
R2297	T3961	T3950	parataxis	30,required
R2298	T3962	T3961	punct	],30
R2299	T3963	T3950	punct	.,required
R23	T230	T231	nsubjpass	Huntingtin,required
R230	T454	T444	punct	),specified
R2300	T3965	T3966	advcl	Expressed,restricted
R2301	T3967	T3968	advmod	just,to
R2302	T3968	T3965	prep	to,Expressed
R2303	T3969	T3968	amod	prior,to
R2304	T3970	T3971	compound	streak,formation
R2305	T3971	T3968	pobj	formation,to
R2306	T3972	T3965	prep	in,Expressed
R2307	T3973	T3974	det	the,epiblast
R2308	T3974	T3972	pobj	epiblast,in
R2309	T3975	T3974	amod	posterior,epiblast
R231	T455	T444	auxpass	are,specified
R2310	T3976	T3974	cc	and,epiblast
R2311	T3977	T3978	amod	visceral,endoderm
R2312	T3978	T3974	conj	endoderm,epiblast
R2313	T3979	T3966	punct	", ",restricted
R2314	T3980	T3966	nsubjpass	Fgf8,restricted
R2315	T3981	T3966	auxpass	is,restricted
R2316	T3982	T3966	prep	to,restricted
R2317	T3983	T3984	det	the,mesoderm
R2318	T3984	T3982	pobj	mesoderm,to
R2319	T3985	T3984	compound	streak,mesoderm
R232	T456	T442	punct	.,shows
R2320	T3986	T3966	prep	at,restricted
R2321	T3987	T3986	pobj	E7.5,at
R2322	T3988	T3966	prep	in,restricted
R2323	T3989	T3990	det	a,gradient
R2324	T3990	T3988	pobj	gradient,in
R2325	T3991	T3990	amod	decreasing,gradient
R2326	T3992	T3993	amod	proximal,distal
R2327	T3993	T3990	amod	distal,gradient
R2328	T3994	T3993	punct	-,distal
R2329	T3995	T3966	cc	and,restricted
R233	T460	T461	nsubj	Huntingtin,is
R2330	T3996	T3997	auxpass	is,downregulated
R2331	T3997	T3966	conj	downregulated,restricted
R2332	T3998	T3997	prep	in,downregulated
R2333	T3999	T3998	pobj	cells,in
R2334	T4000	T4001	advmod	shortly,exit
R2335	T4001	T3997	advcl	exit,downregulated
R2336	T4002	T4001	mark	after,exit
R2337	T4003	T4001	nsubj	they,exit
R2338	T4004	T4005	det	the,streak
R2339	T4005	T4001	dobj	streak,exit
R234	T462	T461	acomp	essential,is
R2340	T4006	T4007	punct	(,G
R2341	T4007	T3997	parataxis	G,downregulated
R2342	T4008	T4007	nmod	Fig.,G
R2343	T4009	T4007	nummod	3E,G
R2344	T4010	T4007	punct	",",G
R2345	T4011	T4007	punct	),G
R2346	T4012	T3966	punct	.,restricted
R2347	T4014	T4015	prep	In,expressed
R2348	T4016	T4017	nmod	Hdhex4,embryos
R2349	T4017	T4014	pobj	embryos,In
R235	T463	T461	prep	in,is
R2350	T4018	T4016	punct	/,Hdhex4
R2351	T4019	T4016	nummod	5,Hdhex4
R2352	T4020	T4016	punct	/,Hdhex4
R2353	T4021	T4016	appos	Hdhex4,Hdhex4
R2354	T4022	T4021	punct	/,Hdhex4
R2355	T4023	T4021	nummod	5,Hdhex4
R2356	T4024	T4015	punct	", ",expressed
R2357	T4025	T4026	compound	Fgf8,expression
R2358	T4026	T4015	nsubjpass	expression,expressed
R2359	T4027	T4015	auxpass	is,expressed
R236	T464	T465	det	the,patterning
R2360	T4028	T4015	advmod	strongly,expressed
R2361	T4029	T4015	prep	in,expressed
R2362	T4030	T4031	det	the,region
R2363	T4031	T4029	pobj	region,in
R2364	T4032	T4031	amod	posterior,region
R2365	T4033	T4031	prep	in,region
R2366	T4034	T4035	det	the,streak
R2367	T4035	T4033	pobj	streak,in
R2368	T4036	T4035	amod	primitive,streak
R2369	T4037	T4029	cc	and,in
R237	T465	T463	pobj	patterning,in
R2370	T4038	T4039	advmod	ectopically,in
R2371	T4039	T4029	conj	in,in
R2372	T4040	T4041	det	the,endoderm
R2373	T4041	T4039	pobj	endoderm,in
R2374	T4042	T4041	acl	overlying,endoderm
R2375	T4043	T4044	det	the,epiblast
R2376	T4044	T4042	dobj	epiblast,overlying
R2377	T4045	T4044	amod	entire,epiblast
R2378	T4046	T4047	punct	(,H
R2379	T4047	T4015	parataxis	H,expressed
R238	T466	T465	amod	early,patterning
R2380	T4048	T4047	nmod	Fig.,H
R2381	T4049	T4047	nummod	3F,H
R2382	T4050	T4047	punct	",",H
R2383	T4051	T4047	punct	),H
R2384	T4052	T4015	punct	.,expressed
R2385	T4054	T4055	advmod	However,appear
R2386	T4056	T4055	punct	", ",appear
R2387	T4057	T4058	compound	streak,derivatives
R2388	T4058	T4055	nsubj	derivatives,appear
R2389	T4059	T4060	aux	to,migrate
R239	T467	T465	prep	of,patterning
R2390	T4060	T4055	xcomp	migrate,appear
R2391	T4061	T4060	advmod	normally,migrate
R2392	T4062	T4063	mark	as,evidenced
R2393	T4063	T4060	advcl	evidenced,migrate
R2394	T4064	T4063	agent	by,evidenced
R2395	T4065	T4066	det	the,expression
R2396	T4066	T4064	pobj	expression,by
R2397	T4067	T4066	amod	proper,expression
R2398	T4068	T4066	amod	anterior,expression
R2399	T4069	T4066	prep	of,expression
R24	T232	T230	punct	", ",Huntingtin
R240	T468	T469	det	the,embryo
R2400	T4070	T4069	pobj	markers,of
R2401	T4071	T4072	amod	such,as
R2402	T4072	T4070	prep	as,markers
R2403	T4073	T4072	pobj	Otx2,as
R2404	T4074	T4073	punct	", ",Otx2
R2405	T4075	T4073	conj	Hnf3β,Otx2
R2406	T4076	T4075	cc	and,Hnf3β
R2407	T4077	T4075	conj	Hesx1,Hnf3β
R2408	T4078	T4066	advmod	anteriorly,expression
R2409	T4079	T4080	punct	(,Fig.
R241	T469	T467	pobj	embryo,of
R2410	T4080	T4063	parataxis	Fig.,evidenced
R2411	T4081	T4080	nummod	1,Fig.
R2412	T4082	T4080	punct	),Fig.
R2413	T4083	T4055	punct	.,appear
R2414	T4085	T4086	advmod	Therefore,exhibit
R2415	T4087	T4086	punct	", ",exhibit
R2416	T4088	T4089	compound	mutant,embryos
R2417	T4089	T4086	nsubj	embryos,exhibit
R2418	T4090	T4091	amod	normal,migration
R2419	T4091	T4086	dobj	migration,exhibit
R242	T470	T461	prep	for,is
R2420	T4092	T4091	prep	of,migration
R2421	T4093	T4094	compound	streak,derivatives
R2422	T4094	T4092	pobj	derivatives,of
R2423	T4095	T4086	cc	but,exhibit
R2424	T4096	T4086	conj	display,exhibit
R2425	T4097	T4098	amod	impaired,repression
R2426	T4098	T4096	dobj	repression,display
R2427	T4099	T4098	compound	Fgf8,repression
R2428	T4100	T4096	prep	in,display
R2429	T4101	T4102	compound	mutant,endoderm
R243	T471	T470	pobj	formation,for
R2430	T4102	T4100	pobj	endoderm,in
R2431	T4103	T4086	punct	.,exhibit
R2432	T4105	T4106	nmod	Hdhex4,embryos
R2433	T4106	T4113	nsubj	embryos,fail
R2434	T4107	T4105	punct	/,Hdhex4
R2435	T4108	T4105	nummod	5,Hdhex4
R2436	T4109	T4105	punct	/,Hdhex4
R2437	T4110	T4105	appos	Hdhex4,Hdhex4
R2438	T4111	T4110	punct	/,Hdhex4
R2439	T4112	T4110	nummod	5,Hdhex4
R244	T472	T471	prep	of,formation
R2440	T4114	T4113	advmod	also,fail
R2441	T4115	T4116	aux	to,restrict
R2442	T4116	T4113	xcomp	restrict,fail
R2443	T4117	T4118	det	the,expression
R2444	T4118	T4116	dobj	expression,restrict
R2445	T4119	T4118	prep	of,expression
R2446	T4120	T4119	pobj	goosecoid,of
R2447	T4121	T4120	punct	(,goosecoid
R2448	T4122	T4120	appos	Gsc,goosecoid
R2449	T4123	T4113	punct	),fail
R245	T473	T474	det	the,region
R2450	T4124	T4113	punct	.,fail
R2451	T4126	T4127	advmod	Normally,expressed
R2452	T4128	T4127	punct	", ",expressed
R2453	T4129	T4127	nsubjpass	Gsc,expressed
R2454	T4130	T4127	auxpass	is,expressed
R2455	T4131	T4127	advmod	initially,expressed
R2456	T4132	T4127	prep	in,expressed
R2457	T4133	T4134	det	the,endoderm
R2458	T4134	T4132	pobj	endoderm,in
R2459	T4135	T4134	amod	visceral,endoderm
R246	T474	T472	pobj	region,of
R2460	T4136	T4134	cc	and,endoderm
R2461	T4137	T4138	amod	proximal,streak
R2462	T4138	T4134	conj	streak,endoderm
R2463	T4139	T4138	punct	", ",streak
R2464	T4140	T4138	amod	posterior,streak
R2465	T4141	T4142	advmod	where,form
R2466	T4142	T4134	relcl	form,endoderm
R2467	T4143	T4144	det	the,streak
R2468	T4144	T4142	nsubj	streak,form
R2469	T4145	T4144	amod	primitive,streak
R247	T475	T474	amod	anterior,region
R2470	T4146	T4142	aux	will,form
R2471	T4147	T4148	advmod	prior,to
R2472	T4148	T4142	prep	to,form
R2473	T4149	T4148	pobj	gastrulation,to
R2474	T4150	T4127	punct	.,expressed
R2475	T4152	T4153	mark	As,forms
R2476	T4153	T4157	advcl	forms,expressed
R2477	T4154	T4155	det	the,streak
R2478	T4155	T4153	nsubj	streak,forms
R2479	T4156	T4155	amod	primitive,streak
R248	T476	T474	prep	of,region
R2480	T4158	T4153	cc	and,forms
R2481	T4159	T4153	conj	extends,forms
R2482	T4160	T4157	punct	", ",expressed
R2483	T4161	T4157	nsubjpass	Gsc,expressed
R2484	T4162	T4157	auxpass	is,expressed
R2485	T4163	T4157	prep	in,expressed
R2486	T4164	T4165	det	the,streak
R2487	T4165	T4163	pobj	streak,in
R2488	T4166	T4165	amod	distal,streak
R2489	T4167	T4165	punct	", ",streak
R249	T477	T478	det	the,streak
R2490	T4168	T4169	det	the,node
R2491	T4169	T4165	conj	node,streak
R2492	T4170	T4169	punct	", ",node
R2493	T4171	T4169	cc	and,node
R2494	T4172	T4173	det	the,mesoderm
R2495	T4173	T4169	conj	mesoderm,node
R2496	T4174	T4173	amod	axial,mesoderm
R2497	T4175	T4173	acl	extending,mesoderm
R2498	T4176	T4177	advmod	anteriorly,from
R2499	T4177	T4175	prep	from,extending
R25	T233	T234	det	the,protein
R250	T478	T476	pobj	streak,of
R2500	T4178	T4179	det	the,node
R2501	T4179	T4177	pobj	node,from
R2502	T4180	T4181	punct	(,32
R2503	T4181	T4157	parataxis	32,expressed
R2504	T4182	T4183	nmod	Fig.,K
R2505	T4183	T4181	dep	K,32
R2506	T4184	T4183	nummod	3I,K
R2507	T4185	T4183	punct	",",K
R2508	T4186	T4181	punct	", ",32
R2509	T4187	T4181	punct	[,32
R251	T479	T478	amod	primitive,streak
R2510	T4188	T4181	nummod	31,32
R2511	T4189	T4181	punct	",",32
R2512	T4190	T4181	punct	],32
R2513	T4191	T4181	punct	),32
R2514	T4192	T4157	punct	.,expressed
R2515	T4194	T4195	advmod	However,remain
R2516	T4196	T4195	punct	", ",remain
R2517	T4197	T4195	prep	in,remain
R2518	T4198	T4199	det	the,embryos
R2519	T4199	T4197	pobj	embryos,in
R252	T480	T470	punct	", ",for
R2520	T4200	T4199	nmod	mutant,embryos
R2521	T4201	T4199	nmod	Hdhex4,embryos
R2522	T4202	T4201	punct	/,Hdhex4
R2523	T4203	T4201	nummod	5,Hdhex4
R2524	T4204	T4201	punct	/,Hdhex4
R2525	T4205	T4201	appos	Hdhex4,Hdhex4
R2526	T4206	T4205	punct	/,Hdhex4
R2527	T4207	T4205	nummod	5,Hdhex4
R2528	T4208	T4195	punct	", ",remain
R2529	T4209	T4210	amod	high,levels
R253	T481	T470	cc	and,for
R2530	T4210	T4195	nsubj	levels,remain
R2531	T4211	T4210	prep	of,levels
R2532	T4212	T4213	compound	Gsc,expression
R2533	T4213	T4211	pobj	expression,of
R2534	T4214	T4195	acomp	unrestricted,remain
R2535	T4215	T4195	prep	in,remain
R2536	T4216	T4217	det	the,endoderm
R2537	T4217	T4215	pobj	endoderm,in
R2538	T4218	T4217	acl	overlying,endoderm
R2539	T4219	T4220	det	the,embryo
R254	T482	T470	conj	for,for
R2540	T4220	T4218	dobj	embryo,overlying
R2541	T4221	T4220	amod	entire,embryo
R2542	T4222	T4215	cc	and,in
R2543	T4223	T4224	advmod	ectopically,in
R2544	T4224	T4215	conj	in,in
R2545	T4225	T4224	pobj	cells,in
R2546	T4226	T4225	amod	adjacent,cells
R2547	T4227	T4226	prep	to,adjacent
R2548	T4228	T4229	det	the,cone
R2549	T4229	T4227	pobj	cone,to
R255	T483	T484	prt	down,regulation
R2550	T4230	T4229	amod	ectoplacental,cone
R2551	T4231	T4232	punct	(,L
R2552	T4232	T4195	parataxis	L,remain
R2553	T4233	T4232	nmod	Fig.,L
R2554	T4234	T4232	nummod	3J,L
R2555	T4235	T4232	punct	",",L
R2556	T4236	T4232	punct	),L
R2557	T4237	T4195	punct	.,remain
R2558	T4239	T4240	det	These,results
R2559	T4240	T4241	nsubj	results,suggest
R256	T484	T482	pobj	regulation,for
R2560	T4242	T4243	mark	that,remains
R2561	T4243	T4241	ccomp	remains,suggest
R2562	T4244	T4243	punct	", ",remains
R2563	T4245	T4243	prep	in,remains
R2564	T4246	T4245	pobj	contrast,in
R2565	T4247	T4246	prep	to,contrast
R2566	T4248	T4249	amod	proper,regulation
R2567	T4249	T4247	pobj	regulation,to
R2568	T4250	T4249	compound	Hnf3β,regulation
R2569	T4251	T4243	punct	", ",remains
R257	T485	T484	punct	-,regulation
R2570	T4252	T4243	nsubj	Gsc,remains
R2571	T4253	T4254	advmod	inappropriately,activated
R2572	T4254	T4243	acomp	activated,remains
R2573	T4255	T4243	prep	in,remains
R2574	T4256	T4257	nmod	mutant,endoderm
R2575	T4257	T4255	pobj	endoderm,in
R2576	T4258	T4257	amod	visceral,endoderm
R2577	T4259	T4258	cc	and,visceral
R2578	T4260	T4258	conj	definitive,visceral
R2579	T4261	T4243	punct	", ",remains
R258	T486	T484	prep	of,regulation
R2580	T4262	T4243	advcl	implicating,remains
R2581	T4263	T4262	dobj	huntingtin,implicating
R2582	T4264	T4262	prep	in,implicating
R2583	T4265	T4266	det	the,restriction
R2584	T4266	T4264	pobj	restriction,in
R2585	T4267	T4266	amod	proper,restriction
R2586	T4268	T4266	prep	of,restriction
R2587	T4269	T4270	det	this,factor
R2588	T4270	T4268	pobj	factor,of
R2589	T4271	T4270	compound	homeodomain,factor
R259	T487	T488	det	a,subset
R2590	T4272	T4270	compound	transcription,factor
R2591	T4273	T4241	punct	.,suggest
R2598	T4743	T4744	nsubjpass	Huntingtin,required
R2599	T4745	T4744	auxpass	is,required
R26	T234	T230	appos	protein,Huntingtin
R260	T488	T486	pobj	subset,of
R2600	T4746	T4744	neg	not,required
R2601	T4747	T4744	prep	for,required
R2602	T4748	T4747	pobj	expression,for
R2603	T4749	T4748	prep	of,expression
R2604	T4750	T4751	amod	extraembryonic,molecules
R2605	T4751	T4749	pobj	molecules,of
R2606	T4752	T4751	compound	signaling,molecules
R2607	T4754	T4755	amod	Previous,studies
R2608	T4755	T4756	nsubj	studies,suggest
R2609	T4757	T4755	prep	of,studies
R261	T489	T488	prep	of,subset
R2610	T4758	T4759	amod	chimeric,embryos
R2611	T4759	T4757	pobj	embryos,of
R2612	T4760	T4761	mark	that,required
R2613	T4761	T4756	ccomp	required,suggest
R2614	T4762	T4761	nsubjpass	huntingtin,required
R2615	T4763	T4761	auxpass	is,required
R2616	T4764	T4765	advmod	only,in
R2617	T4765	T4761	prep	in,required
R2618	T4766	T4767	det	the,tissue
R2619	T4767	T4765	pobj	tissue,in
R262	T490	T491	amod	dynamic,genes
R2620	T4768	T4767	amod	extraembryonic,tissue
R2621	T4769	T4761	prep	for,required
R2622	T4770	T4771	amod	proper,development
R2623	T4771	T4769	pobj	development,for
R2624	T4772	T4773	punct	[,16
R2625	T4773	T4761	parataxis	16,required
R2626	T4774	T4773	punct	],16
R2627	T4775	T4756	punct	.,suggest
R2628	T4777	T4778	nsubj	Signals,are
R2629	T4779	T4777	prep	from,Signals
R263	T491	T489	pobj	genes,of
R2630	T4780	T4781	det	the,tissue
R2631	T4781	T4779	pobj	tissue,from
R2632	T4782	T4781	amod	extraembryonic,tissue
R2633	T4783	T4778	acomp	critical,are
R2634	T4784	T4783	prep	for,critical
R2635	T4785	T4786	det	the,induction
R2636	T4786	T4784	pobj	induction,for
R2637	T4787	T4786	prep	of,induction
R2638	T4788	T4789	amod	embryonic,signals
R2639	T4789	T4787	pobj	signals,of
R264	T492	T493	nmod	growth,factor
R2640	T4790	T4784	cc	and,for
R2641	T4791	T4784	conj	for,for
R2642	T4792	T4791	pcomp	patterning,for
R2643	T4793	T4794	det	the,epiblast
R2644	T4794	T4792	dobj	epiblast,patterning
R2645	T4795	T4778	punct	.,are
R2646	T4797	T4798	advmod	Consequently,examined
R2647	T4799	T4798	punct	", ",examined
R2648	T4800	T4798	nsubj	we,examined
R2649	T4801	T4802	amod	extraembryonic,development
R265	T493	T491	compound	factor,genes
R2650	T4802	T4798	dobj	development,examined
R2651	T4803	T4798	prep	in,examined
R2652	T4804	T4805	npadvmod	huntingtin,deficient
R2653	T4805	T4806	amod	deficient,embryos
R2654	T4806	T4803	pobj	embryos,in
R2655	T4807	T4798	punct	.,examined
R2656	T4809	T4810	nsubj	Hnf4,is
R2657	T4811	T4812	det	a,factor
R2658	T4812	T4810	attr	factor,is
R2659	T4813	T4812	compound	transcription,factor
R266	T494	T492	cc	and,growth
R2660	T4814	T4812	acl	expressed,factor
R2661	T4815	T4814	prep	in,expressed
R2662	T4816	T4817	det	the,endoderm
R2663	T4817	T4815	pobj	endoderm,in
R2664	T4818	T4817	amod	primitive,endoderm
R2665	T4819	T4820	advmod	as,soon
R2666	T4820	T4814	advmod	soon,expressed
R2667	T4821	T4822	mark	as,becomes
R2668	T4822	T4820	advcl	becomes,soon
R2669	T4823	T4824	det	this,tissue
R267	T495	T492	conj	transcription,growth
R2670	T4824	T4822	nsubj	tissue,becomes
R2671	T4825	T4822	acomp	distinct,becomes
R2672	T4826	T4822	cc	and,becomes
R2673	T4827	T4822	conj	is,becomes
R2674	T4828	T4829	det	a,regulator
R2675	T4829	T4827	attr	regulator,is
R2676	T4830	T4829	amod	key,regulator
R2677	T4831	T4829	prep	of,regulator
R2678	T4832	T4833	amod	visceral,endoderm
R2679	T4833	T4834	npadvmod	endoderm,secreted
R268	T496	T461	punct	.,is
R2680	T4834	T4835	amod	secreted,factors
R2681	T4835	T4831	pobj	factors,of
R2682	T4836	T4837	amod	such,as
R2683	T4837	T4835	prep	as,factors
R2684	T4838	T4837	pobj	alphafetoprotein,as
R2685	T4839	T4838	punct	", ",alphafetoprotein
R2686	T4840	T4838	conj	apolipoproteins,alphafetoprotein
R2687	T4841	T4840	punct	", ",apolipoproteins
R2688	T4842	T4840	cc	and,apolipoproteins
R2689	T4843	T4840	conj	transferrin,apolipoproteins
R269	T498	T499	det	These,findings
R2690	T4844	T4810	punct	.,is
R2691	T4846	T4847	nsubj	Inactivation,results
R2692	T4848	T4846	prep	of,Inactivation
R2693	T4849	T4848	pobj	Hnf4,of
R2694	T4850	T4847	prep	in,results
R2695	T4851	T4852	amod	impaired,gastrulation
R2696	T4852	T4850	pobj	gastrulation,in
R2697	T4853	T4854	punct	[,34
R2698	T4854	T4847	parataxis	34,results
R2699	T4855	T4854	nummod	33,34
R27	T235	T234	nmod	HD,protein
R270	T499	T500	nsubj	findings,provide
R2700	T4856	T4854	punct	",",34
R2701	T4857	T4854	punct	],34
R2702	T4858	T4847	punct	.,results
R2703	T4860	T4861	prep	At,expressed
R2704	T4862	T4860	pobj	E7.5,At
R2705	T4863	T4861	punct	", ",expressed
R2706	T4864	T4861	nsubjpass	Hnf4,expressed
R2707	T4865	T4861	auxpass	is,expressed
R2708	T4866	T4861	prep	in,expressed
R2709	T4867	T4868	det	the,cells
R271	T501	T502	amod	fundamental,points
R2710	T4868	T4866	pobj	cells,in
R2711	T4869	T4870	amod	columnar,endoderm
R2712	T4870	T4868	compound	endoderm,cells
R2713	T4871	T4870	amod	visceral,endoderm
R2714	T4872	T4861	prep	at,expressed
R2715	T4873	T4874	det	the,junction
R2716	T4874	T4872	pobj	junction,at
R2717	T4875	T4876	amod	extraembryonic,ectoderm
R2718	T4876	T4874	compound	ectoderm,junction
R2719	T4877	T4876	punct	-,ectoderm
R272	T502	T500	dobj	points,provide
R2720	T4878	T4879	punct	(,33
R2721	T4879	T4861	parataxis	33,expressed
R2722	T4880	T4879	dep	Fig.,33
R2723	T4881	T4880	nummod	4A,Fig.
R2724	T4882	T4879	punct	", ",33
R2725	T4883	T4879	punct	[,33
R2726	T4884	T4879	punct	],33
R2727	T4885	T4879	punct	),33
R2728	T4886	T4861	punct	.,expressed
R2729	T4888	T4889	prep	In,appears
R273	T503	T502	amod	starting,points
R2730	T4890	T4891	nmod	Hdhex4,embryos
R2731	T4891	T4888	pobj	embryos,In
R2732	T4892	T4890	punct	/,Hdhex4
R2733	T4893	T4890	nummod	5,Hdhex4
R2734	T4894	T4890	punct	/,Hdhex4
R2735	T4895	T4890	appos	Hdhex4,Hdhex4
R2736	T4896	T4895	punct	/,Hdhex4
R2737	T4897	T4895	nummod	5,Hdhex4
R2738	T4898	T4889	punct	", ",appears
R2739	T4899	T4889	advcl	consistent,appears
R274	T504	T502	prep	for,points
R2740	T4900	T4899	prep	with,consistent
R2741	T4901	T4902	amod	normal,differentiation
R2742	T4902	T4900	pobj	differentiation,with
R2743	T4903	T4902	amod	primitive,differentiation
R2744	T4904	T4903	cc	and,primitive
R2745	T4905	T4903	conj	visceral,primitive
R2746	T4906	T4902	compound	endoderm,differentiation
R2747	T4907	T4889	punct	", ",appears
R2748	T4908	T4909	compound	Hnf4,expression
R2749	T4909	T4889	nsubj	expression,appears
R275	T505	T504	pcomp	identifying,for
R2750	T4910	T4889	oprd	normal,appears
R2751	T4911	T4889	punct	", ",appears
R2752	T4912	T4913	mark	although,is
R2753	T4913	T4889	advcl	is,appears
R2754	T4914	T4915	det	the,signal
R2755	T4915	T4913	nsubj	signal,is
R2756	T4916	T4913	acomp	stronger,is
R2757	T4917	T4913	prep	in,is
R2758	T4918	T4919	compound	mutant,embryos
R2759	T4919	T4917	pobj	embryos,in
R276	T506	T507	det	the,activities
R2760	T4920	T4913	prep	compared,is
R2761	T4921	T4920	prep	to,compared
R2762	T4922	T4923	amod	wild,type
R2763	T4923	T4925	compound	type,embryos
R2764	T4924	T4923	punct	-,type
R2765	T4925	T4921	pobj	embryos,to
R2766	T4926	T4927	punct	(,Fig.
R2767	T4927	T4889	parataxis	Fig.,appears
R2768	T4928	T4927	nummod	4B,Fig.
R2769	T4929	T4927	punct	),Fig.
R277	T507	T505	dobj	activities,identifying
R2770	T4930	T4889	punct	.,appears
R2771	T4932	T4933	advmod	Similarly,expressed
R2772	T4934	T4933	punct	", ",expressed
R2773	T4935	T4933	nsubjpass	Pem,expressed
R2774	T4936	T4935	punct	", ",Pem
R2775	T4937	T4938	det	a,factor
R2776	T4938	T4935	appos	factor,Pem
R2777	T4939	T4938	compound	transcription,factor
R2778	T4940	T4938	acl	expressed,factor
R2779	T4941	T4940	prep	in,expressed
R278	T508	T507	amod	novel,activities
R2780	T4942	T4943	amod	proximal,endoderm
R2781	T4943	T4941	pobj	endoderm,in
R2782	T4944	T4943	amod	visceral,endoderm
R2783	T4945	T4943	cc	and,endoderm
R2784	T4946	T4947	amod	ectoplacental,cone
R2785	T4947	T4943	conj	cone,endoderm
R2786	T4948	T4940	prep	in,expressed
R2787	T4949	T4950	amod	wild,type
R2788	T4950	T4952	compound	type,embryos
R2789	T4951	T4950	punct	-,type
R279	T509	T507	amod	cellular,activities
R2790	T4952	T4948	pobj	embryos,in
R2791	T4953	T4940	prep	at,expressed
R2792	T4954	T4953	pobj	E7.5,at
R2793	T4955	T4933	punct	", ",expressed
R2794	T4956	T4933	advmod	also,expressed
R2795	T4957	T4933	auxpass	is,expressed
R2796	T4958	T4933	prep	in,expressed
R2797	T4959	T4960	det	these,tissues
R2798	T4960	T4958	pobj	tissues,in
R2799	T4961	T4933	prep	in,expressed
R28	T236	T237	npadvmod	gene,encoded
R280	T510	T509	cc	and,cellular
R2800	T4962	T4963	det	the,embryos
R2801	T4963	T4961	pobj	embryos,in
R2802	T4964	T4963	compound	mutant,embryos
R2803	T4965	T4966	punct	(,35
R2804	T4966	T4933	parataxis	35,expressed
R2805	T4967	T4968	nmod	Fig.,D
R2806	T4968	T4966	dep	D,35
R2807	T4969	T4968	nummod	4C,D
R2808	T4970	T4968	punct	",",D
R2809	T4971	T4966	punct	[,35
R281	T511	T509	conj	molecular,cellular
R2810	T4972	T4966	punct	],35
R2811	T4973	T4966	punct	),35
R2812	T4974	T4933	punct	.,expressed
R2813	T4976	T4977	advmod	However,hangs
R2814	T4978	T4977	punct	", ",hangs
R2815	T4979	T4977	nsubj	Pem,hangs
R2816	T4980	T4979	acl	expressing,Pem
R2817	T4981	T4982	amod	visceral,endoderm
R2818	T4982	T4980	dobj	endoderm,expressing
R2819	T4983	T4977	prep	over,hangs
R282	T512	T507	prep	of,activities
R2820	T4984	T4985	det	the,anterior
R2821	T4985	T4983	pobj	anterior,over
R2822	T4986	T4985	prep	of,anterior
R2823	T4987	T4988	det	the,embryos
R2824	T4988	T4986	pobj	embryos,of
R2825	T4989	T4988	compound	mutant,embryos
R2826	T4990	T4977	punct	", ",hangs
R2827	T4991	T4977	advcl	revealing,hangs
R2828	T4992	T4993	amod	abnormal,location
R2829	T4993	T4991	dobj	location,revealing
R283	T513	T512	pobj	huntingtin,of
R2830	T4994	T4991	prep	despite,revealing
R2831	T4995	T4996	advmod	grossly,normal
R2832	T4996	T4997	amod	normal,differentiation
R2833	T4997	T4994	pobj	differentiation,despite
R2834	T4998	T4977	punct	.,hangs
R2835	T5007	T5008	amod	r,om
R2836	T5008	T5006	pobj	om,f
R2837	T5009	T5010	det	the,e
R2838	T5018	T5019	compound	u,es
R2839	T5027	T5026	prep	in,ud
R284	T514	T505	prep	in,identifying
R2840	T5028	T5026	advcl	g,ud
R2841	T5029	T5030	det	the,an
R2842	T5031	T5032	det	te,or
R2843	T5033	T5032	amod	ri,or
R2844	T5040	T5039	advcl	ce,l
R2845	T5042	T5039	dobj	e,l
R2846	T5052	T5051	advcl	d,n
R2847	T5056	T5055	nsubj	x,t
R2848	T5060	T5059	pobj	e,r
R2849	T5061	T5060	amod	aembryonic,e
R285	T515	T516	det	the,tissues
R2850	T5062	T5060	prep	c,e
R2851	T5063	T5064	det	t,derm
R2852	T5064	T5062	pobj	derm,c
R2853	T5065	T5064	amod	o,derm
R2854	T5066	T5064	cc	a,derm
R2855	T5067	T5068	amod	re,r
R2856	T5068	T5064	conj	r,derm
R2857	T5069	T5058	auxpass	e,quired
R2858	T5070	T5058	prep	for,quired
R2859	T5071	T5072	amod	proper,formation
R286	T516	T514	pobj	tissues,in
R2860	T5072	T5070	pobj	formation,for
R2861	T5073	T5072	cc	and,formation
R2862	T5074	T5072	conj	patterning,formation
R2863	T5075	T5072	prep	of,formation
R2864	T5076	T5077	det	the,epiblast
R2865	T5077	T5075	pobj	epiblast,of
R2866	T5078	T5079	punct	[,17
R2867	T5079	T5058	parataxis	17,quired
R2868	T5080	T5079	punct	],17
R2869	T5081	T5058	punct	.,quired
R287	T517	T516	amod	extraembryonic,tissues
R2870	T5083	T5084	nsubj	Bmp4,is
R2871	T5085	T5086	det	a,molecule
R2872	T5086	T5084	attr	molecule,is
R2873	T5087	T5086	compound	signaling,molecule
R2874	T5088	T5089	dep	that,expressed
R2875	T5089	T5086	relcl	expressed,molecule
R2876	T5090	T5089	auxpass	is,expressed
R2877	T5091	T5089	advmod	first,expressed
R2878	T5092	T5089	advmod	uniformly,expressed
R2879	T5093	T5089	prep	throughout,expressed
R288	T518	T519	dep	that,govern
R2880	T5094	T5095	det	the,ectoderm
R2881	T5095	T5093	pobj	ectoderm,throughout
R2882	T5096	T5095	amod	extraembryonic,ectoderm
R2883	T5097	T5089	cc	and,expressed
R2884	T5098	T5099	advmod	subsequently,localized
R2885	T5099	T5089	conj	localized,expressed
R2886	T5100	T5099	auxpass	is,localized
R2887	T5101	T5099	prep	to,localized
R2888	T5102	T5103	det	a,ring
R2889	T5103	T5101	pobj	ring,to
R289	T519	T516	relcl	govern,tissues
R2890	T5104	T5103	prep	of,ring
R2891	T5105	T5106	amod	extraembryonic,ectoderm
R2892	T5106	T5104	pobj	ectoderm,of
R2893	T5107	T5103	amod	adjacent,ring
R2894	T5108	T5107	prep	to,adjacent
R2895	T5109	T5110	det	the,epiblast
R2896	T5110	T5108	pobj	epiblast,to
R2897	T5111	T5112	punct	(,36
R2898	T5112	T5099	parataxis	36,localized
R2899	T5113	T5112	dep	Fig.,36
R29	T237	T234	amod	encoded,protein
R290	T520	T521	amod	normal,development
R2900	T5114	T5113	nummod	4E,Fig.
R2901	T5115	T5112	punct	", ",36
R2902	T5116	T5112	punct	[,36
R2903	T5117	T5112	punct	],36
R2904	T5118	T5112	punct	),36
R2905	T5119	T5084	punct	.,is
R2906	T5121	T5122	det	A,factor
R2907	T5122	T5124	dep	factor,required
R2908	T5123	T5122	amod	key,factor
R2909	T5125	T5122	prep	in,factor
R291	T521	T519	dobj	development,govern
R2910	T5126	T5125	pcomp	regulating,in
R2911	T5127	T5128	det	the,formation
R2912	T5128	T5126	dobj	formation,regulating
R2913	T5129	T5128	prep	of,formation
R2914	T5130	T5131	det	the,node
R2915	T5131	T5129	pobj	node,of
R2916	T5132	T5131	cc	and,node
R2917	T5133	T5134	amod	primitive,streak
R2918	T5134	T5131	conj	streak,node
R2919	T5135	T5124	punct	", ",required
R292	T522	T523	amod	anterior,streak
R2920	T5136	T5124	nsubjpass	Bmp4,required
R2921	T5137	T5124	auxpass	is,required
R2922	T5138	T5124	prep	for,required
R2923	T5139	T5138	pcomp	patterning,for
R2924	T5140	T5141	det	the,embryo
R2925	T5141	T5139	dobj	embryo,patterning
R2926	T5142	T5139	prep	along,patterning
R2927	T5143	T5144	det	the,axis
R2928	T5144	T5142	pobj	axis,along
R2929	T5145	T5144	amod	proximodistal,axis
R293	T523	T521	compound	streak,development
R2930	T5146	T5147	punct	[,37
R2931	T5147	T5124	parataxis	37,required
R2932	T5148	T5149	punct	-,40
R2933	T5149	T5147	prep	40,37
R2934	T5150	T5147	punct	],37
R2935	T5151	T5124	punct	.,required
R2936	T5153	T5154	prep	In,maintained
R2937	T5155	T5156	det	the,absence
R2938	T5156	T5153	pobj	absence,In
R2939	T5157	T5156	prep	of,absence
R294	T524	T500	punct	.,provide
R2940	T5158	T5157	pobj	huntingtin,of
R2941	T5159	T5154	punct	", ",maintained
R2942	T5160	T5161	compound	Bmp4,expression
R2943	T5161	T5154	nsubjpass	expression,maintained
R2944	T5162	T5154	auxpass	is,maintained
R2945	T5163	T5154	advmod	properly,maintained
R2946	T5164	T5154	prep	in,maintained
R2947	T5165	T5166	det	the,ectoderm
R2948	T5166	T5164	pobj	ectoderm,in
R2949	T5167	T5166	nmod	Hdhex4,ectoderm
R295	T526	T527	det	This,knowledge
R2950	T5168	T5167	punct	/,Hdhex4
R2951	T5169	T5167	nummod	5,Hdhex4
R2952	T5170	T5167	punct	/,Hdhex4
R2953	T5171	T5167	appos	Hdhex4,Hdhex4
R2954	T5172	T5171	punct	/,Hdhex4
R2955	T5173	T5171	nummod	5,Hdhex4
R2956	T5174	T5166	amod	extraembryonic,ectoderm
R2957	T5175	T5154	cc	but,maintained
R2958	T5176	T5177	auxpass	is,expressed
R2959	T5177	T5154	conj	expressed,maintained
R296	T527	T528	nsubj	knowledge,prove
R2960	T5178	T5177	advmod	also,expressed
R2961	T5179	T5177	prep	throughout,expressed
R2962	T5180	T5181	det	the,ectoderm
R2963	T5181	T5179	pobj	ectoderm,throughout
R2964	T5182	T5181	amod	extraembryonic,ectoderm
R2965	T5183	T5184	punct	(,Fig.
R2966	T5184	T5181	parataxis	Fig.,ectoderm
R2967	T5185	T5184	nummod	4F,Fig.
R2968	T5186	T5184	punct	),Fig.
R2969	T5187	T5177	prep	in,expressed
R297	T529	T528	aux	may,prove
R2970	T5188	T5189	det	a,pattern
R2971	T5189	T5187	pobj	pattern,in
R2972	T5190	T5191	dep	that,is
R2973	T5191	T5189	relcl	is,pattern
R2974	T5192	T5191	acomp	similar,is
R2975	T5193	T5192	prep	to,similar
R2976	T5194	T5195	amod	early,expression
R2977	T5195	T5193	pobj	expression,to
R2978	T5196	T5195	compound	Bmp4,expression
R2979	T5197	T5198	advmod	rather,than
R298	T530	T531	aux	to,be
R2980	T5198	T5177	prep	than,expressed
R2981	T5199	T5200	auxpass	being,restricted
R2982	T5200	T5198	pcomp	restricted,than
R2983	T5201	T5200	prep	to,restricted
R2984	T5202	T5203	det	a,ring
R2985	T5203	T5201	pobj	ring,to
R2986	T5204	T5203	prep	of,ring
R2987	T5205	T5206	amod	extraembryonic,ectoderm
R2988	T5206	T5204	pobj	ectoderm,of
R2989	T5207	T5208	mark	as,seen
R299	T531	T528	xcomp	be,prove
R2990	T5208	T5200	advcl	seen,restricted
R2991	T5209	T5208	prep	in,seen
R2992	T5210	T5211	det	the,embryos
R2993	T5211	T5209	pobj	embryos,in
R2994	T5212	T5213	amod	wild,type
R2995	T5213	T5211	compound	type,embryos
R2996	T5214	T5213	punct	-,type
R2997	T5216	T5217	aux	To,assess
R2998	T5217	T5218	advcl	assess,evaluated
R2999	T5219	T5220	compound	Bmp4,signaling
R30	T238	T234	acl	mutated,protein
R300	T532	T531	acomp	important,be
R3000	T5220	T5217	dobj	signaling,assess
R3001	T5221	T5220	prep	from,signaling
R3002	T5222	T5223	det	the,ectoderm
R3003	T5223	T5221	pobj	ectoderm,from
R3004	T5224	T5223	amod	extraembryonic,ectoderm
R3005	T5225	T5218	punct	", ",evaluated
R3006	T5226	T5218	nsubj	we,evaluated
R3007	T5227	T5228	amod	primordial,cells
R3008	T5228	T5218	dobj	cells,evaluated
R3009	T5229	T5228	compound	germ,cells
R301	T533	T532	prep	for,important
R3010	T5230	T5228	punct	(,cells
R3011	T5231	T5228	appos	PGCs,cells
R3012	T5232	T5228	punct	),cells
R3013	T5233	T5228	punct	", ",cells
R3014	T5234	T5235	dep	which,require
R3015	T5235	T5228	relcl	require,cells
R3016	T5236	T5235	dobj	Bmp4,require
R3017	T5237	T5235	prep	for,require
R3018	T5238	T5239	poss	their,induction
R3019	T5239	T5237	pobj	induction,for
R302	T534	T533	pcomp	understanding,for
R3020	T5240	T5241	punct	[,37
R3021	T5241	T5218	parataxis	37,evaluated
R3022	T5242	T5241	punct	],37
R3023	T5243	T5218	punct	.,evaluated
R3024	T5245	T5246	nsubjpass	PGCs,detected
R3025	T5247	T5246	aux	can,detected
R3026	T5248	T5246	advmod	first,detected
R3027	T5249	T5246	auxpass	be,detected
R3028	T5250	T5246	prep	at,detected
R3029	T5251	T5250	pobj	E7.0,at
R303	T535	T536	det	the,mechanism
R3030	T5252	T5246	cc	and,detected
R3031	T5253	T5254	advmod	subsequently,underlie
R3032	T5254	T5246	conj	underlie,detected
R3033	T5255	T5256	det	the,portion
R3034	T5256	T5254	dobj	portion,underlie
R3035	T5257	T5256	amod	posterior,portion
R3036	T5258	T5256	prep	of,portion
R3037	T5259	T5260	det	the,streak
R3038	T5260	T5258	pobj	streak,of
R3039	T5261	T5260	amod	primitive,streak
R304	T536	T534	dobj	mechanism,understanding
R3040	T5262	T5246	punct	.,detected
R3041	T5264	T5265	amod	Whole,mount
R3042	T5265	T5266	compound	mount,staining
R3043	T5266	T5267	nsubj	staining,reveals
R3044	T5268	T5266	prep	of,staining
R3045	T5269	T5270	nmod	E7.5,embryos
R3046	T5270	T5268	pobj	embryos,of
R3047	T5271	T5270	nmod	mutant,embryos
R3048	T5272	T5271	cc	and,mutant
R3049	T5273	T5274	amod	wild,type
R305	T537	T538	prep	by,determines
R3050	T5274	T5271	conj	type,mutant
R3051	T5275	T5274	punct	-,type
R3052	T5276	T5266	prep	for,staining
R3053	T5277	T5278	compound	alkaline,phosphatase
R3054	T5278	T5279	compound	phosphatase,activity
R3055	T5279	T5276	pobj	activity,for
R3056	T5280	T5281	mark	that,form
R3057	T5281	T5267	ccomp	form,reveals
R3058	T5282	T5281	nsubj	PCGs,form
R3059	T5283	T5281	prep	in,form
R306	T538	T536	relcl	determines,mechanism
R3060	T5284	T5285	nmod	Hdhex4,embryos
R3061	T5285	T5283	pobj	embryos,in
R3062	T5286	T5284	punct	/,Hdhex4
R3063	T5287	T5284	nummod	5,Hdhex4
R3064	T5288	T5284	punct	/,Hdhex4
R3065	T5289	T5284	appos	Hdhex4,Hdhex4
R3066	T5290	T5289	punct	/,Hdhex4
R3067	T5291	T5289	nummod	5,Hdhex4
R3068	T5292	T5281	punct	", ",form
R3069	T5293	T5281	advcl	suggesting,form
R307	T539	T537	pobj	which,by
R3070	T5294	T5295	mark	that,is
R3071	T5295	T5293	ccomp	is,suggesting
R3072	T5296	T5297	compound	Bmp4,signaling
R3073	T5297	T5295	nsubj	signaling,is
R3074	T5298	T5295	acomp	functional,is
R3075	T5299	T5295	prep	in,is
R3076	T5300	T5301	det	the,absence
R3077	T5301	T5299	pobj	absence,in
R3078	T5302	T5301	prep	of,absence
R3079	T5303	T5302	pobj	huntingtin,of
R308	T540	T541	det	the,expansion
R3080	T5304	T5305	punct	(,H
R3081	T5305	T5281	parataxis	H,form
R3082	T5306	T5305	nmod	Fig.,H
R3083	T5307	T5305	nummod	4G,H
R3084	T5308	T5305	punct	",",H
R3085	T5309	T5305	punct	),H
R3086	T5310	T5267	punct	.,reveals
R3087	T5312	T5313	det	The,endoderm
R3088	T5313	T5316	nsubj	endoderm,is
R3089	T5314	T5313	amod	anterior,endoderm
R309	T541	T538	nsubj	expansion,determines
R3090	T5315	T5313	amod	visceral,endoderm
R3091	T5317	T5313	punct	(,endoderm
R3092	T5318	T5313	appos	AVE,endoderm
R3093	T5319	T5316	punct	),is
R3094	T5320	T5316	advmod	also,is
R3095	T5321	T5322	det	an,source
R3096	T5322	T5316	attr	source,is
R3097	T5323	T5322	amod	extraembryonic,source
R3098	T5324	T5322	prep	of,source
R3099	T5325	T5324	pobj	signals,of
R31	T239	T238	agent	by,mutated
R310	T542	T541	amod	dominant,expansion
R3100	T5326	T5327	dep	that,are
R3101	T5327	T5325	relcl	are,signals
R3102	T5328	T5327	acomp	critical,are
R3103	T5329	T5328	prep	for,critical
R3104	T5330	T5331	amod	early,patterning
R3105	T5331	T5329	pobj	patterning,for
R3106	T5332	T5316	punct	.,is
R3107	T5334	T5335	nsubjpass	Wnt,expressed
R3108	T5336	T5334	cc	and,Wnt
R3109	T5337	T5338	compound	nodal,antagonists
R311	T543	T541	compound	polyglutamine,expansion
R3110	T5338	T5334	conj	antagonists,Wnt
R3111	T5339	T5338	punct	", ",antagonists
R3112	T5340	T5338	appos	Dkk1,antagonists
R3113	T5341	T5342	punct	(,mdkk
R3114	T5342	T5340	parataxis	mdkk,Dkk1
R3115	T5343	T5342	punct	-,mdkk
R3116	T5344	T5342	nummod	1,mdkk
R3117	T5345	T5342	punct	),mdkk
R3118	T5346	T5340	cc	and,Dkk1
R3119	T5347	T5340	conj	Lefty1,Dkk1
R312	T544	T541	prep	in,expansion
R3120	T5348	T5340	advmod	respectively,Dkk1
R3121	T5349	T5335	punct	", ",expressed
R3122	T5350	T5335	auxpass	are,expressed
R3123	T5351	T5335	prep	in,expressed
R3124	T5352	T5353	det	the,AVE
R3125	T5353	T5351	pobj	AVE,in
R3126	T5354	T5335	cc	and,expressed
R3127	T5355	T5335	conj	are,expressed
R3128	T5356	T5355	acomp	important,are
R3129	T5357	T5356	prep	in,important
R313	T545	T544	pobj	huntingtin,in
R3130	T5358	T5357	pcomp	limiting,in
R3131	T5359	T5360	det	the,posteriorization
R3132	T5360	T5358	dobj	posteriorization,limiting
R3133	T5361	T5360	prep	of,posteriorization
R3134	T5362	T5363	det	the,embryo
R3135	T5363	T5361	pobj	embryo,of
R3136	T5364	T5363	amod	anterior,embryo
R3137	T5365	T5358	prep	by,limiting
R3138	T5366	T5365	pcomp	restricting,by
R3139	T5367	T5368	nmod	Nodal,signaling
R314	T546	T547	det	the,loss
R3140	T5368	T5366	dobj	signaling,restricting
R3141	T5369	T5367	cc	and,Nodal
R3142	T5370	T5367	conj	Wnt,Nodal
R3143	T5371	T5372	punct	[,41
R3144	T5372	T5355	parataxis	41,are
R3145	T5373	T5374	punct	-,43
R3146	T5374	T5372	prep	43,41
R3147	T5375	T5372	punct	],41
R3148	T5376	T5335	punct	.,expressed
R3149	T5378	T5379	prep	In,expressed
R315	T547	T538	dobj	loss,determines
R3150	T5380	T5381	nmod	Hdhex4,embryos
R3151	T5381	T5378	pobj	embryos,In
R3152	T5382	T5380	punct	/,Hdhex4
R3153	T5383	T5380	nummod	5,Hdhex4
R3154	T5384	T5380	punct	/,Hdhex4
R3155	T5385	T5380	appos	Hdhex4,Hdhex4
R3156	T5386	T5385	punct	/,Hdhex4
R3157	T5387	T5385	nummod	5,Hdhex4
R3158	T5388	T5379	punct	", ",expressed
R3159	T5389	T5390	preconj	both,Dkk1
R316	T548	T547	prep	of,loss
R3160	T5390	T5379	nsubjpass	Dkk1,expressed
R3161	T5391	T5392	punct	(,J
R3162	T5392	T5390	parataxis	J,Dkk1
R3163	T5393	T5392	nmod	Fig.,J
R3164	T5394	T5392	nummod	4I,J
R3165	T5395	T5392	punct	",",J
R3166	T5396	T5392	punct	),J
R3167	T5397	T5390	cc	and,Dkk1
R3168	T5398	T5390	conj	Lefty1,Dkk1
R3169	T5399	T5400	punct	(,L
R317	T549	T548	pobj	neurons,of
R3170	T5400	T5398	parataxis	L,Lefty1
R3171	T5401	T5400	nmod	Fig.,L
R3172	T5402	T5400	nummod	4K,L
R3173	T5403	T5400	punct	",",L
R3174	T5404	T5400	punct	),L
R3175	T5405	T5379	auxpass	are,expressed
R3176	T5406	T5379	advmod	normally,expressed
R3177	T5407	T5379	prep	in,expressed
R3178	T5408	T5409	det	the,AVE
R3179	T5409	T5407	pobj	AVE,in
R318	T550	T547	prep	in,loss
R3180	T5410	T5411	mark	as,compared
R3181	T5411	T5379	advcl	compared,expressed
R3182	T5412	T5411	prep	with,compared
R3183	T5413	T5414	amod	wild,type
R3184	T5414	T5416	compound	type,embryos
R3185	T5415	T5414	punct	-,type
R3186	T5416	T5412	pobj	embryos,with
R3187	T5417	T5379	punct	.,expressed
R3188	T5419	T5420	advmod	However,appear
R3189	T5421	T5420	punct	", ",appear
R319	T551	T552	poss	Huntington,disease
R3190	T5422	T5423	nmod	Dkk,levels
R3191	T5423	T5420	nsubj	levels,appear
R3192	T5424	T5422	punct	-,Dkk
R3193	T5425	T5422	nummod	1,Dkk
R3194	T5426	T5427	aux	to,increased
R3195	T5427	T5420	xcomp	increased,appear
R3196	T5428	T5427	auxpass	be,increased
R3197	T5429	T5427	advmod	slightly,increased
R3198	T5430	T5427	prep	in,increased
R3199	T5431	T5432	nmod	Hdhex4,embryos
R32	T240	T241	compound	polyglutamine,expansion
R320	T552	T550	pobj	disease,in
R3200	T5432	T5430	pobj	embryos,in
R3201	T5433	T5431	punct	/,Hdhex4
R3202	T5434	T5431	nummod	5,Hdhex4
R3203	T5435	T5431	punct	/,Hdhex4
R3204	T5436	T5431	appos	Hdhex4,Hdhex4
R3205	T5437	T5436	punct	/,Hdhex4
R3206	T5438	T5436	nummod	5,Hdhex4
R3207	T5439	T5427	punct	", ",increased
R3208	T5440	T5441	mark	although,remains
R3209	T5441	T5427	advcl	remains,increased
R321	T553	T551	case	's,Huntington
R3210	T5442	T5443	det	the,pattern
R3211	T5443	T5441	nsubj	pattern,remains
R3212	T5444	T5443	prep	of,pattern
R3213	T5445	T5446	nmod	Dkk,expression
R3214	T5446	T5444	pobj	expression,of
R3215	T5447	T5445	punct	-,Dkk
R3216	T5448	T5445	nummod	1,Dkk
R3217	T5449	T5441	acomp	unchanged,remains
R3218	T5450	T5441	cc	and,remains
R3219	T5451	T5452	det	this,increase
R322	T554	T528	punct	.,prove
R3220	T5452	T5453	nsubj	increase,reflect
R3221	T5453	T5441	conj	reflect,remains
R3222	T5454	T5453	aux	may,reflect
R3223	T5455	T5453	advmod	just,reflect
R3224	T5456	T5457	det	the,amount
R3225	T5457	T5453	dobj	amount,reflect
R3226	T5458	T5457	amod	same,amount
R3227	T5459	T5457	prep	of,amount
R3228	T5460	T5459	pobj	expression,of
R3229	T5461	T5457	prep	in,amount
R323	T204	T205	nsubj	Inactivation,impairs
R3230	T5462	T5463	det	a,area
R3231	T5463	T5461	pobj	area,in
R3232	T5464	T5463	amod	smaller,area
R3233	T5465	T5420	punct	.,appear
R3234	T5467	T5468	advmod	Therefore,appear
R3235	T5469	T5468	punct	", ",appear
R3236	T5470	T5471	det	the,expression
R3237	T5471	T5468	nsubj	expression,appear
R3238	T5472	T5471	amod	ectopic,expression
R3239	T5473	T5471	prep	of,expression
R3240	T5474	T5473	pobj	Nodal,of
R3241	T5475	T5476	punct	(,Fig.
R3242	T5476	T5474	parataxis	Fig.,Nodal
R3243	T5477	T5476	nummod	3A,Fig.
R3244	T5478	T5479	punct	–,D
R3245	T5479	T5476	prep	D,Fig.
R3246	T5480	T5476	punct	),Fig.
R3247	T5481	T5471	cc	and,expression
R3248	T5482	T5483	det	the,expression
R3249	T5483	T5471	conj	expression,expression
R3250	T5484	T5483	amod	decreased,expression
R3251	T5485	T5483	compound	Wnt3a,expression
R3252	T5486	T5487	punct	(,Fig.
R3253	T5487	T5483	parataxis	Fig.,expression
R3254	T5488	T5487	nummod	2H,Fig.
R3255	T5489	T5487	punct	),Fig.
R3256	T5490	T5471	prep	in,expression
R3257	T5491	T5492	compound	mutant,embryos
R3258	T5492	T5490	pobj	embryos,in
R3259	T5493	T5468	aux	do,appear
R3260	T5494	T5468	neg	not,appear
R3261	T5495	T5496	aux	to,be
R3262	T5496	T5468	xcomp	be,appear
R3263	T5497	T5496	attr	result,be
R3264	T5498	T5497	prep	of,result
R3265	T5499	T5498	pobj	changes,of
R3266	T5500	T5499	prep	in,changes
R3267	T5501	T5502	det	the,pattern
R3268	T5502	T5500	pobj	pattern,in
R3269	T5503	T5502	compound	expression,pattern
R3270	T5504	T5502	prep	of,pattern
R3271	T5505	T5504	pobj	Lefty1,of
R3272	T5506	T5505	cc	or,Lefty1
R3273	T5507	T5505	conj	Dkk1,Lefty1
R3274	T5508	T5468	punct	.,appear
R3275	T5510	T5511	prep	Despite,form
R3276	T5512	T5513	amod	normal,formation
R3277	T5513	T5510	pobj	formation,Despite
R3278	T5514	T5513	compound	AVE,formation
R3279	T5515	T5513	cc	and,formation
R3280	T5516	T5517	compound	neuroectoderm,induction
R3281	T5517	T5513	conj	induction,formation
R3282	T5518	T5511	punct	", ",form
R3283	T5519	T5520	compound	head,folds
R3284	T5520	T5511	nsubj	folds,form
R3285	T5521	T5511	aux	do,form
R3286	T5522	T5511	neg	not,form
R3287	T5523	T5511	prep	in,form
R3288	T5524	T5525	nmod	Hdhex4,embryos
R3289	T5525	T5523	pobj	embryos,in
R3290	T5526	T5524	punct	/,Hdhex4
R3291	T5527	T5524	nummod	5,Hdhex4
R3292	T5528	T5524	punct	/,Hdhex4
R3293	T5529	T5524	appos	Hdhex4,Hdhex4
R3294	T5530	T5529	punct	/,Hdhex4
R3295	T5531	T5529	nummod	5,Hdhex4
R3296	T5532	T5511	punct	.,form
R3297	T5534	T5535	advmod	Therefore,allowed
R3298	T5536	T5535	punct	", ",allowed
R3299	T5537	T5538	aux	to,determine
R33	T241	T239	pobj	expansion,by
R3300	T5538	T5535	advcl	determine,allowed
R3301	T5539	T5540	mark	whether,are
R3302	T5540	T5538	ccomp	are,determine
R3303	T5541	T5542	compound	mutant,embryos
R3304	T5542	T5540	nsubj	embryos,are
R3305	T5543	T5544	advmod	inherently,capable
R3306	T5544	T5540	acomp	capable,are
R3307	T5545	T5544	prep	of,capable
R3308	T5546	T5545	pcomp	forming,of
R3309	T5547	T5548	compound	head,folds
R3310	T5548	T5546	dobj	folds,forming
R3311	T5549	T5535	punct	", ",allowed
R3312	T5550	T5535	nsubjpass	embryos,allowed
R3313	T5551	T5550	acl	harvested,embryos
R3314	T5552	T5551	prep	at,harvested
R3315	T5553	T5554	compound	stage,E7.5
R3316	T5554	T5552	pobj	E7.5,at
R3317	T5555	T5535	auxpass	were,allowed
R3318	T5556	T5557	aux	to,progress
R3319	T5557	T5535	xcomp	progress,allowed
R3320	T5558	T5557	prep	in,progress
R3321	T5559	T5560	amod	rich,medium
R3322	T5560	T5558	pobj	medium,in
R3323	T5561	T5560	compound	culture,medium
R3324	T5562	T5563	advmod	in,vitro
R3325	T5563	T5557	advmod	vitro,progress
R3326	T5564	T5557	prep	for,progress
R3327	T5565	T5566	nummod	24,hours
R3328	T5566	T5564	pobj	hours,for
R3329	T5567	T5535	punct	.,allowed
R3330	T5569	T5570	amod	Wild,type
R3331	T5570	T5572	compound	type,embryos
R3332	T5571	T5570	punct	-,type
R3333	T5572	T5573	nsubj	embryos,continued
R3334	T5574	T5575	aux	to,develop
R3335	T5575	T5573	xcomp	develop,continued
R3336	T5576	T5577	compound	head,folds
R3337	T5577	T5575	dobj	folds,develop
R3338	T5578	T5577	punct	", ",folds
R3339	T5579	T5577	conj	somites,folds
R3340	T5580	T5579	cc	and,somites
R3341	T5581	T5579	conj	hearts,somites
R3342	T5582	T5583	punct	(,N
R3343	T5583	T5575	parataxis	N,develop
R3344	T5584	T5583	nmod	Fig.,N
R3345	T5585	T5583	nummod	4M,N
R3346	T5586	T5583	punct	",",N
R3347	T5587	T5583	punct	),N
R3348	T5588	T5573	punct	.,continued
R3349	T5590	T5591	prep	In,develop
R3350	T5592	T5590	pobj	contrast,In
R3351	T5593	T5591	punct	", ",develop
R3352	T5594	T5595	nmod	mutant,embryos
R3353	T5595	T5591	nsubj	embryos,develop
R3354	T5596	T5595	nmod	stage,embryos
R3355	T5597	T5596	nummod	7.5,stage
R3356	T5598	T5591	aux	did,develop
R3357	T5599	T5591	neg	not,develop
R3358	T5600	T5591	dobj	headfolds,develop
R3359	T5601	T5600	punct	", ",headfolds
R3360	T5602	T5600	conj	hearts,headfolds
R3361	T5603	T5602	cc	or,hearts
R3362	T5604	T5602	conj	somites,hearts
R3363	T5605	T5591	punct	", ",develop
R3364	T5606	T5607	mark	although,continued
R3365	T5607	T5591	advcl	continued,develop
R3366	T5608	T5609	det	these,embryos
R3367	T5609	T5607	nsubj	embryos,continued
R3368	T5610	T5611	aux	to,live
R3369	T5611	T5607	xcomp	live,continued
R337	T927	T928	poss	Huntington,disease
R3370	T5612	T5613	punct	(,Fig.
R3371	T5613	T5591	parataxis	Fig.,develop
R3372	T5614	T5613	nummod	4O,Fig.
R3373	T5615	T5613	punct	),Fig.
R3374	T5616	T5591	punct	.,develop
R3375	T5618	T5619	det	These,results
R3376	T5619	T5620	nsubj	results,suggest
R3377	T5621	T5620	advmod	strongly,suggest
R3378	T5622	T5623	mark	that,are
R3379	T5623	T5620	ccomp	are,suggest
R338	T928	T930	nsubj	disease,is
R3380	T5624	T5623	prep	in,are
R3381	T5625	T5626	det	the,absence
R3382	T5626	T5624	pobj	absence,in
R3383	T5627	T5626	prep	of,absence
R3384	T5628	T5627	pobj	huntingtin,of
R3385	T5629	T5623	punct	", ",are
R3386	T5630	T5623	nsubj	embryos,are
R3387	T5631	T5623	acomp	unable,are
R3388	T5632	T5633	aux	to,undergo
R3389	T5633	T5631	xcomp	undergo,unable
R339	T929	T927	case	's,Huntington
R3390	T5634	T5633	dobj	organogenesis,undergo
R3391	T5635	T5633	punct	", ",undergo
R3392	T5636	T5637	advmod	even,continue
R3393	T5637	T5633	advcl	continue,undergo
R3394	T5638	T5637	mark	if,continue
R3395	T5639	T5637	nsubj	they,continue
R3396	T5640	T5641	aux	to,live
R3397	T5641	T5637	xcomp	live,continue
R3398	T5642	T5641	prep	past,live
R3399	T5643	T5642	pobj	E7.5,past
R34	T242	T238	prep	in,mutated
R340	T931	T928	punct	(,disease
R3400	T5644	T5641	prep	in,live
R3401	T5645	T5646	det	a,environment
R3402	T5646	T5644	pobj	environment,in
R3403	T5647	T5648	npadvmod	nutrient,rich
R3404	T5648	T5646	amod	rich,environment
R3405	T5649	T5620	punct	.,suggest
R3408	T6261	T6262	nsubj	We,investigated
R3409	T6263	T6262	aux	have,investigated
R341	T932	T928	appos	HD,disease
R3410	T6264	T6265	det	the,processes
R3411	T6265	T6262	dobj	processes,investigated
R3412	T6266	T6265	amod	embryonic,processes
R3413	T6267	T6268	dep	that,require
R3414	T6268	T6265	relcl	require,processes
R3415	T6269	T6268	dobj	huntingtin,require
R3416	T6270	T6268	prep	in,require
R3417	T6271	T6270	pobj	order,in
R3418	T6272	T6273	aux	to,delineate
R3419	T6273	T6271	acl	delineate,order
R342	T933	T930	punct	),is
R3420	T6274	T6275	advmod	more,precisely
R3421	T6275	T6273	advmod	precisely,delineate
R3422	T6276	T6277	poss	huntingtin,activities
R3423	T6277	T6273	dobj	activities,delineate
R3424	T6278	T6276	case	's,huntingtin
R3425	T6279	T6277	amod	essential,activities
R3426	T6280	T6277	amod	molecular,activities
R3427	T6281	T6280	cc	and,molecular
R3428	T6282	T6280	conj	cellular,molecular
R3429	T6283	T6273	cc	and,delineate
R343	T934	T935	det	a,disorder
R3430	T6284	T6285	aux	to,provide
R3431	T6285	T6273	conj	provide,delineate
R3432	T6286	T6285	dobj	clues,provide
R3433	T6287	T6286	prep	to,clues
R3434	T6288	T6289	det	the,mechanism
R3435	T6289	T6287	pobj	mechanism,to
R3436	T6290	T6291	prep	by,leads
R3437	T6291	T6289	relcl	leads,mechanism
R3438	T6292	T6290	pobj	which,by
R3439	T6293	T6294	det	the,mutation
R344	T935	T930	attr	disorder,is
R3440	T6294	T6291	nsubj	mutation,leads
R3441	T6295	T6294	amod	dominant,mutation
R3442	T6296	T6297	compound	polyglutamine,expansion
R3443	T6297	T6294	compound	expansion,mutation
R3444	T6298	T6294	prep	in,mutation
R3445	T6299	T6298	pobj	huntingtin,in
R3446	T6300	T6291	prep	to,leads
R3447	T6301	T6302	compound	HD,pathogenesis
R3448	T6302	T6300	pobj	pathogenesis,to
R3449	T6303	T6262	punct	.,investigated
R345	T936	T937	advmod	dominantly,inherited
R3450	T6305	T6306	prep	In,fail
R3451	T6307	T6305	pcomp	pursuing,In
R3452	T6308	T6309	det	the,finding
R3453	T6309	T6307	dobj	finding,pursuing
R3454	T6310	T6311	mark	that,needed
R3455	T6311	T6309	acl	needed,finding
R3456	T6312	T6311	nsubjpass	huntingtin,needed
R3457	T6313	T6311	auxpass	is,needed
R3458	T6314	T6315	advmod	only,in
R3459	T6315	T6311	prep	in,needed
R346	T937	T935	amod	inherited,disorder
R3460	T6316	T6317	amod	extraembryonic,tissues
R3461	T6317	T6315	pobj	tissues,in
R3462	T6318	T6311	prep	for,needed
R3463	T6319	T6320	amod	normal,gastrulation
R3464	T6320	T6318	pobj	gastrulation,for
R3465	T6321	T6306	punct	", ",fail
R3466	T6322	T6323	poss	our,data
R3467	T6323	T6306	nsubj	data,fail
R3468	T6324	T6325	aux	to,provide
R3469	T6325	T6306	xcomp	provide,fail
R347	T938	T935	amod	neurodegenerative,disorder
R3470	T6326	T6325	dobj	evidence,provide
R3471	T6327	T6326	prep	of,evidence
R3472	T6328	T6329	amod	abnormal,expression
R3473	T6329	T6327	pobj	expression,of
R3474	T6330	T6331	compound	nutritive,gene
R3475	T6331	T6329	compound	gene,expression
R3476	T6332	T6329	prep	in,expression
R3477	T6333	T6334	nmod	Hdhex4,embryos
R3478	T6334	T6332	pobj	embryos,in
R3479	T6335	T6333	punct	/,Hdhex4
R348	T939	T940	dep	that,caused
R3480	T6336	T6333	nummod	5,Hdhex4
R3481	T6337	T6333	punct	/,Hdhex4
R3482	T6338	T6333	appos	Hdhex4,Hdhex4
R3483	T6339	T6338	punct	/,Hdhex4
R3484	T6340	T6338	nummod	5,Hdhex4
R3485	T6341	T6306	punct	.,fail
R3486	T6343	T6344	advmod	Instead,reveal
R3487	T6345	T6344	punct	", ",reveal
R3488	T6346	T6347	poss	our,results
R3489	T6347	T6344	nsubj	results,reveal
R349	T940	T935	relcl	caused,disorder
R3490	T6348	T6349	mark	that,required
R3491	T6349	T6344	ccomp	required,reveal
R3492	T6350	T6349	nsubjpass	huntingtin,required
R3493	T6351	T6349	auxpass	is,required
R3494	T6352	T6349	prep	for,required
R3495	T6353	T6354	amod	normal,formation
R3496	T6354	T6352	pobj	formation,for
R3497	T6355	T6356	amod	anterior,streak
R3498	T6356	T6354	compound	streak,formation
R3499	T6357	T6354	cc	and,formation
R35	T243	T244	poss	Huntington,disease
R350	T941	T940	auxpass	is,caused
R3500	T6358	T6359	det	the,production
R3501	T6359	T6354	conj	production,formation
R3502	T6360	T6359	amod	consequent,production
R3503	T6361	T6359	prep	of,production
R3504	T6362	T6363	amod	paraxial,mesoderm
R3505	T6363	T6361	pobj	mesoderm,of
R3506	T6364	T6349	punct	", ",required
R3507	T6365	T6349	prep	with,required
R3508	T6366	T6367	det	a,role
R3509	T6367	T6365	pobj	role,with
R351	T942	T940	agent	by,caused
R3510	T6368	T6369	advmod	previously,unrecognized
R3511	T6369	T6367	amod	unrecognized,role
R3512	T6370	T6367	prep	for,role
R3513	T6371	T6370	pobj	huntingtin,for
R3514	T6372	T6367	prep	in,role
R3515	T6373	T6374	det	the,extinction
R3516	T6374	T6372	pobj	extinction,in
R3517	T6375	T6374	amod	proper,extinction
R3518	T6376	T6374	prep	of,extinction
R3519	T6377	T6378	advmod	transiently,expressed
R352	T943	T944	compound	CAG,repeats
R3520	T6378	T6383	amod	expressed,genes
R3521	T6379	T6377	cc	and,transiently
R3522	T6380	T6379	punct	/,and
R3523	T6381	T6379	cc	or,and
R3524	T6382	T6377	conj	dynamically,transiently
R3525	T6383	T6376	pobj	genes,of
R3526	T6384	T6344	punct	.,reveal
R3527	T6386	T6387	advmod	Indeed,is
R3528	T6388	T6387	punct	", ",is
R3529	T6389	T6390	det	the,hallmark
R353	T944	T942	pobj	repeats,by
R3530	T6390	T6387	nsubj	hallmark,is
R3531	T6391	T6390	prep	of,hallmark
R3532	T6392	T6393	det	the,phenotype
R3533	T6393	T6391	pobj	phenotype,of
R3534	T6394	T6395	npadvmod	huntingtin,deficient
R3535	T6395	T6393	amod	deficient,phenotype
R3536	T6396	T6393	amod	molecular,phenotype
R3537	T6397	T6398	det	the,regulation
R3538	T6398	T6387	attr	regulation,is
R3539	T6399	T6398	amod	impaired,regulation
R354	T945	T944	prep	in,repeats
R3540	T6400	T6398	compound	down,regulation
R3541	T6401	T6398	punct	-,regulation
R3542	T6402	T6398	prep	of,regulation
R3543	T6403	T6404	det	a,subset
R3544	T6404	T6402	pobj	subset,of
R3545	T6405	T6404	prep	of,subset
R3546	T6406	T6407	advmod	dynamically,expressed
R3547	T6407	T6408	amod	expressed,genes
R3548	T6408	T6405	pobj	genes,of
R3549	T6409	T6398	punct	", ",regulation
R355	T946	T947	det	the,locus
R3550	T6410	T6398	prep	after,regulation
R3551	T6411	T6412	det	the,onset
R3552	T6412	T6410	pobj	onset,after
R3553	T6413	T6412	amod	proper,onset
R3554	T6414	T6412	prep	of,onset
R3555	T6415	T6414	pobj	expression,of
R3556	T6416	T6387	punct	.,is
R3557	T6418	T6419	det	This,phenomenon
R3558	T6419	T6420	nsubj	phenomenon,reflect
R3559	T6421	T6420	aux	does,reflect
R356	T947	T945	pobj	locus,in
R3560	T6422	T6420	neg	not,reflect
R3561	T6423	T6424	det	a,lack
R3562	T6424	T6420	dobj	lack,reflect
R3563	T6425	T6424	prep	of,lack
R3564	T6426	T6427	amod	anterior,posterior
R3565	T6427	T6429	amod	posterior,formation
R3566	T6428	T6427	punct	/,posterior
R3567	T6429	T6425	pobj	formation,of
R3568	T6430	T6429	compound	axis,formation
R3569	T6431	T6420	punct	", ",reflect
R357	T948	T947	compound	HD,locus
R3570	T6432	T6433	mark	as,evidenced
R3571	T6433	T6420	advcl	evidenced,reflect
R3572	T6434	T6433	agent	by,evidenced
R3573	T6435	T6436	det	the,formation
R3574	T6436	T6434	pobj	formation,by
R3575	T6437	T6436	prep	of,formation
R3576	T6438	T6439	det	the,AVE
R3577	T6439	T6437	pobj	AVE,of
R3578	T6440	T6436	advmod	anteriorly,formation
R3579	T6441	T6436	cc	and,formation
R358	T949	T950	dep	that,extend
R3580	T6442	T6443	det	the,streak
R3581	T6443	T6436	conj	streak,formation
R3582	T6444	T6443	amod	primitive,streak
R3583	T6445	T6443	advmod	posteriorly,streak
R3584	T6446	T6420	punct	.,reflect
R3585	T6448	T6449	cc	Nor,explained
R3586	T6450	T6449	aux	can,explained
R3587	T6451	T6449	nsubjpass	it,explained
R3588	T6452	T6449	auxpass	be,explained
R3589	T6453	T6449	advmod	simply,explained
R359	T950	T944	relcl	extend,repeats
R3590	T6454	T6449	agent	by,explained
R3591	T6455	T6456	amod	delayed,development
R3592	T6456	T6454	pobj	development,by
R3593	T6457	T6449	punct	", ",explained
R3594	T6458	T6459	mark	as,induced
R3595	T6459	T6449	advcl	induced,explained
R3596	T6460	T6461	npadvmod	stage,specific
R3597	T6461	T6463	amod	specific,markers
R3598	T6462	T6461	punct	-,specific
R3599	T6463	T6459	nsubjpass	markers,induced
R36	T244	T242	pobj	disease,in
R360	T951	T952	det	a,tract
R3600	T6464	T6463	punct	", ",markers
R3601	T6465	T6466	amod	such,as
R3602	T6466	T6463	prep	as,markers
R3603	T6467	T6466	pobj	Wnt3a,as
R3604	T6468	T6467	cc	and,Wnt3a
R3605	T6469	T6470	amod	primordial,cells
R3606	T6470	T6467	conj	cells,Wnt3a
R3607	T6471	T6470	compound	germ,cells
R3608	T6472	T6467	punct	", ",Wnt3a
R3609	T6473	T6474	dep	which,are
R361	T952	T950	dobj	tract,extend
R3610	T6474	T6467	relcl	are,Wnt3a
R3611	T6475	T6474	acomp	detectable,are
R3612	T6476	T6474	prep	at,are
R3613	T6477	T6476	pobj	E7.0,at
R3614	T6478	T6474	prep	in,are
R3615	T6479	T6480	amod	wild,type
R3616	T6480	T6482	compound	type,embryos
R3617	T6481	T6480	punct	-,type
R3618	T6482	T6478	pobj	embryos,in
R3619	T6483	T6459	punct	", ",induced
R362	T953	T952	compound	polyglutamine,tract
R3620	T6484	T6459	auxpass	are,induced
R3621	T6485	T6459	advmod	appropriately,induced
R3622	T6486	T6449	punct	.,explained
R3623	T6488	T6489	advmod	Furthermore,is
R3624	T6490	T6489	punct	", ",is
R3625	T6491	T6492	det	the,expression
R3626	T6492	T6489	nsubj	expression,is
R3627	T6493	T6492	prep	of,expression
R3628	T6494	T6493	pobj	T,of
R3629	T6495	T6494	cc	and,T
R363	T954	T950	prep	in,extend
R3630	T6496	T6494	conj	Evx1,T
R3631	T6497	T6492	prep	in,expression
R3632	T6498	T6499	det	the,mesoderm
R3633	T6499	T6497	pobj	mesoderm,in
R3634	T6500	T6499	amod	extraembryonic,mesoderm
R3635	T6501	T6499	prep	of,mesoderm
R3636	T6502	T6503	compound	mutant,embryos
R3637	T6503	T6501	pobj	embryos,of
R3638	T6504	T6489	neg	not,is
R3639	T6505	T6506	det	a,feature
R364	T955	T956	det	a,protein
R3640	T6506	T6489	attr	feature,is
R3641	T6507	T6506	prep	of,feature
R3642	T6508	T6509	amod	wild,type
R3643	T6509	T6511	compound	type,embryos
R3644	T6510	T6509	punct	-,type
R3645	T6511	T6507	pobj	embryos,of
R3646	T6512	T6489	punct	", ",is
R3647	T6513	T6514	advmod	even,at
R3648	T6514	T6489	prep	at,is
R3649	T6515	T6516	amod	earlier,stages
R365	T956	T954	pobj	protein,in
R3650	T6516	T6514	pobj	stages,at
R3651	T6517	T6489	punct	.,is
R3652	T6519	T6520	nsubj	This,suggests
R3653	T6521	T6520	advmod	strongly,suggests
R3654	T6522	T6523	mark	that,occurs
R3655	T6523	T6520	advcl	occurs,suggests
R3656	T6524	T6523	prep	in,occurs
R3657	T6525	T6526	npadvmod	huntingtin,deficient
R3658	T6526	T6527	amod	deficient,embryos
R3659	T6527	T6524	pobj	embryos,in
R366	T957	T956	amod	ubiquitous,protein
R3660	T6528	T6523	punct	", ",occurs
R3661	T6529	T6530	det	the,migration
R3662	T6530	T6523	nsubj	migration,occurs
R3663	T6531	T6530	prep	of,migration
R3664	T6532	T6533	det	the,derivatives
R3665	T6533	T6531	pobj	derivatives,of
R3666	T6534	T6535	amod	distal,streak
R3667	T6535	T6533	compound	streak,derivatives
R3668	T6536	T6530	prep	to,migration
R3669	T6537	T6538	det	the,mesoderm
R367	T958	T959	compound	HEAT,domain
R3670	T6538	T6536	pobj	mesoderm,to
R3671	T6539	T6538	amod	extraembryonic,mesoderm
R3672	T6540	T6523	advmod	normally,occurs
R3673	T6541	T6523	cc	but,occurs
R3674	T6542	T6543	mark	that,impaired
R3675	T6543	T6523	conj	impaired,occurs
R3676	T6544	T6545	det	the,regulation
R3677	T6545	T6543	nsubjpass	regulation,impaired
R3678	T6546	T6545	compound	down,regulation
R3679	T6547	T6545	punct	-,regulation
R368	T959	T956	compound	domain,protein
R3680	T6548	T6545	prep	of,regulation
R3681	T6549	T6550	det	these,genes
R3682	T6550	T6548	pobj	genes,of
R3683	T6551	T6543	auxpass	is,impaired
R3684	T6552	T6520	punct	.,suggests
R3685	T6554	T6555	det	This,impairment
R3686	T6555	T6556	nsubj	impairment,explain
R3687	T6557	T6556	aux	may,explain
R3688	T6558	T6556	advmod	also,explain
R3689	T6559	T6560	det	the,failure
R369	T960	T956	acl	called,protein
R3690	T6560	T6556	dobj	failure,explain
R3691	T6561	T6560	prep	of,failure
R3692	T6562	T6563	npadvmod	huntingtin,deficient
R3693	T6563	T6564	amod	deficient,embryos
R3694	T6564	T6561	pobj	embryos,of
R3695	T6565	T6566	aux	to,restrict
R3696	T6566	T6560	acl	restrict,failure
R3697	T6567	T6566	advmod	properly,restrict
R3698	T6568	T6569	det	the,expression
R3699	T6569	T6566	dobj	expression,restrict
R37	T245	T243	case	's,Huntington
R370	T961	T960	oprd	huntingtin,called
R3700	T6570	T6569	prep	of,expression
R3701	T6571	T6570	pobj	Fgf8,of
R3702	T6572	T6571	punct	", ",Fgf8
R3703	T6573	T6571	conj	Nodal,Fgf8
R3704	T6574	T6573	cc	and,Nodal
R3705	T6575	T6573	conj	Gsc,Nodal
R3706	T6576	T6556	punct	.,explain
R3707	T6578	T6579	advmod	Thus,play
R3708	T6580	T6579	punct	", ",play
R3709	T6581	T6579	nsubj	huntingtin,play
R371	T962	T963	punct	[,1
R3710	T6582	T6579	aux	may,play
R3711	T6583	T6584	det	a,role
R3712	T6584	T6579	dobj	role,play
R3713	T6585	T6584	amod	direct,role
R3714	T6586	T6579	prep	in,play
R3715	T6587	T6588	det	the,regulation
R3716	T6588	T6586	pobj	regulation,in
R3717	T6589	T6588	amod	transcriptional,regulation
R3718	T6590	T6588	punct	", ",regulation
R3719	T6591	T6588	cc	or,regulation
R372	T963	T950	parataxis	1,extend
R3720	T6592	T6593	compound	mRNA,stability
R3721	T6593	T6588	conj	stability,regulation
R3722	T6594	T6588	prep	of,regulation
R3723	T6595	T6596	det	these,genes
R3724	T6596	T6594	pobj	genes,of
R3725	T6597	T6579	cc	or,play
R3726	T6598	T6599	nsubj	it,act
R3727	T6599	T6579	conj	act,play
R3728	T6600	T6599	aux	may,act
R3729	T6601	T6599	advmod	indirectly,act
R373	T964	T963	punct	],1
R3730	T6602	T6599	prep	by,act
R3731	T6603	T6602	pcomp	intersecting,by
R3732	T6604	T6603	prep	with,intersecting
R3733	T6605	T6606	amod	other,pathways
R3734	T6606	T6604	pobj	pathways,with
R3735	T6607	T6608	dep	that,regulate
R3736	T6608	T6606	relcl	regulate,pathways
R3737	T6609	T6610	det	the,expression
R3738	T6610	T6608	dobj	expression,regulate
R3739	T6611	T6610	prep	of,expression
R374	T965	T930	punct	.,is
R3740	T6612	T6613	det	these,genes
R3741	T6613	T6611	pobj	genes,of
R3742	T6614	T6599	punct	.,act
R3743	T6616	T6617	det	The,requirement
R3744	T6617	T6618	nsubj	requirement,prompted
R3745	T6619	T6617	prep	for,requirement
R3746	T6620	T6619	pobj	huntingtin,for
R3747	T6621	T6617	prep	in,requirement
R3748	T6622	T6623	det	the,tissues
R3749	T6623	T6621	pobj	tissues,in
R375	T967	T968	det	The,mechanism
R3750	T6624	T6623	amod	extraembryonic,tissues
R3751	T6625	T6618	aux	had,prompted
R3752	T6626	T6618	dobj	us,prompted
R3753	T6627	T6628	aux	to,test
R3754	T6628	T6618	xcomp	test,prompted
R3755	T6629	T6630	mark	whether,be
R3756	T6630	T6628	ccomp	be,test
R3757	T6631	T6632	amod	impaired,signals
R3758	T6632	T6630	nsubj	signals,be
R3759	T6633	T6632	amod	extraembryonic,signals
R376	T968	T970	nsubjpass	mechanism,known
R3760	T6634	T6630	aux	might,be
R3761	T6635	T6630	acomp	responsible,be
R3762	T6636	T6635	prep	for,responsible
R3763	T6637	T6638	det	the,dysregulation
R3764	T6638	T6636	pobj	dysregulation,for
R3765	T6639	T6638	prep	of,dysregulation
R3766	T6640	T6641	compound	gene,expression
R3767	T6641	T6639	pobj	expression,of
R3768	T6642	T6638	prep	within,dysregulation
R3769	T6643	T6644	det	the,epiblast
R377	T969	T968	amod	molecular,mechanism
R3770	T6644	T6642	pobj	epiblast,within
R3771	T6645	T6646	dep	that,observed
R3772	T6646	T6644	relcl	observed,epiblast
R3773	T6647	T6646	auxpass	is,observed
R3774	T6648	T6646	prep	in,observed
R3775	T6649	T6650	nmod	Hdhex4,embryos
R3776	T6650	T6648	pobj	embryos,in
R3777	T6651	T6649	punct	/,Hdhex4
R3778	T6652	T6649	nummod	5,Hdhex4
R3779	T6653	T6649	punct	/,Hdhex4
R378	T971	T972	prep	by,leads
R3780	T6654	T6649	appos	Hdhex4,Hdhex4
R3781	T6655	T6654	punct	/,Hdhex4
R3782	T6656	T6654	nummod	5,Hdhex4
R3783	T6657	T6618	punct	.,prompted
R3784	T6659	T6660	amod	Extraembryonic,development
R3785	T6660	T6661	nsubjpass	development,associated
R3786	T6662	T6660	prep	in,development
R3787	T6663	T6664	nmod	Hdhex4,embryos
R3788	T6664	T6662	pobj	embryos,in
R3789	T6665	T6663	punct	/,Hdhex4
R379	T972	T968	relcl	leads,mechanism
R3790	T6666	T6663	nummod	5,Hdhex4
R3791	T6667	T6663	punct	/,Hdhex4
R3792	T6668	T6663	appos	Hdhex4,Hdhex4
R3793	T6669	T6668	punct	/,Hdhex4
R3794	T6670	T6668	nummod	5,Hdhex4
R3795	T6671	T6661	auxpass	is,associated
R3796	T6672	T6661	prep	with,associated
R3797	T6673	T6674	advmod	mildly,elevated
R3798	T6674	T6675	amod	elevated,expression
R3799	T6675	T6672	pobj	expression,with
R38	T246	T231	punct	", ",required
R380	T973	T971	pobj	which,by
R3800	T6676	T6675	compound	levels,expression
R3801	T6677	T6675	prep	of,expression
R3802	T6678	T6677	pobj	Hnf4,of
R3803	T6679	T6678	prep	in,Hnf4
R3804	T6680	T6681	det	the,endoderm
R3805	T6681	T6679	pobj	endoderm,in
R3806	T6682	T6681	amod	primitive,endoderm
R3807	T6683	T6678	cc	and,Hnf4
R3808	T6684	T6678	conj	Pem,Hnf4
R3809	T6685	T6684	prep	in,Pem
R381	T974	T975	det	the,property
R3810	T6686	T6687	det	the,chorion
R3811	T6687	T6685	pobj	chorion,in
R3812	T6688	T6687	amod	lopsided,chorion
R3813	T6689	T6687	amod	anterior,chorion
R3814	T6690	T6661	cc	but,associated
R3815	T6691	T6692	det	the,expression
R3816	T6692	T6693	nsubj	expression,appear
R3817	T6693	T6661	conj	appear,associated
R3818	T6694	T6692	prep	of,expression
R3819	T6695	T6696	amod	other,signals
R382	T975	T972	nsubj	property,leads
R3820	T6696	T6694	pobj	signals,of
R3821	T6697	T6696	amod	known,signals
R3822	T6698	T6696	punct	", ",signals
R3823	T6699	T6700	amod	such,as
R3824	T6700	T6696	prep	as,signals
R3825	T6701	T6700	pobj	Bmp4,as
R3826	T6702	T6701	prep	from,Bmp4
R3827	T6703	T6704	det	the,ectoderm
R3828	T6704	T6702	pobj	ectoderm,from
R3829	T6705	T6704	amod	extraembryonic,ectoderm
R383	T976	T975	amod	new,property
R3830	T6706	T6701	punct	", ",Bmp4
R3831	T6707	T6701	cc	and,Bmp4
R3832	T6708	T6701	conj	Dkk1,Bmp4
R3833	T6709	T6708	cc	and,Dkk1
R3834	T6710	T6708	conj	Lefty1,Dkk1
R3835	T6711	T6708	prep	from,Dkk1
R3836	T6712	T6713	det	the,AVE
R3837	T6713	T6711	pobj	AVE,from
R3838	T6714	T6693	punct	", ",appear
R3839	T6715	T6716	aux	to,be
R384	T977	T978	dep	that,conferred
R3840	T6716	T6693	xcomp	be,appear
R3841	T6717	T6716	acomp	normal,be
R3842	T6718	T6693	punct	", ",appear
R3843	T6719	T6720	mark	although,suggests
R3844	T6720	T6693	advcl	suggests,appear
R3845	T6721	T6722	det	the,increase
R3846	T6722	T6720	nsubj	increase,suggests
R3847	T6723	T6722	amod	slight,increase
R3848	T6724	T6722	prep	in,increase
R3849	T6725	T6726	nmod	Dkk,expression
R385	T978	T975	relcl	conferred,property
R3850	T6726	T6724	pobj	expression,in
R3851	T6727	T6725	punct	-,Dkk
R3852	T6728	T6725	nummod	1,Dkk
R3853	T6729	T6722	prep	in,increase
R3854	T6730	T6731	nmod	Hdhex4,embryos
R3855	T6731	T6729	pobj	embryos,in
R3856	T6732	T6730	punct	/,Hdhex4
R3857	T6733	T6730	nummod	5,Hdhex4
R3858	T6734	T6730	punct	/,Hdhex4
R3859	T6735	T6730	appos	Hdhex4,Hdhex4
R386	T979	T978	auxpass	is,conferred
R3860	T6736	T6735	punct	/,Hdhex4
R3861	T6737	T6735	nummod	5,Hdhex4
R3862	T6738	T6739	mark	that,is
R3863	T6739	T6720	ccomp	is,suggests
R3864	T6740	T6741	amod	further,investigation
R3865	T6741	T6739	nsubj	investigation,is
R3866	T6742	T6741	prep	into,investigation
R3867	T6743	T6744	compound	Wnt,signaling
R3868	T6744	T6742	pobj	signaling,into
R3869	T6745	T6739	acomp	warranted,is
R387	T980	T978	prep	on,conferred
R3870	T6746	T6693	punct	.,appear
R3871	T6748	T6749	advmod	Moreover,impaired
R3872	T6750	T6749	punct	", ",impaired
R3873	T6751	T6752	amod	extraembryonic,signaling
R3874	T6752	T6749	nsubjpass	signaling,impaired
R3875	T6753	T6752	compound	Bmp4,signaling
R3876	T6754	T6749	auxpass	is,impaired
R3877	T6755	T6749	neg	not,impaired
R3878	T6756	T6749	prep	in,impaired
R3879	T6757	T6758	det	the,absence
R388	T981	T980	pobj	huntingtin,on
R3880	T6758	T6756	pobj	absence,in
R3881	T6759	T6758	prep	of,absence
R3882	T6760	T6759	pobj	huntingtin,of
R3883	T6761	T6749	punct	", ",impaired
R3884	T6762	T6763	mark	as,is
R3885	T6763	T6749	advcl	is,impaired
R3886	T6764	T6765	det	the,induction
R3887	T6765	T6763	nsubj	induction,is
R3888	T6766	T6765	prep	of,induction
R3889	T6767	T6766	pobj	PCGs,of
R389	T982	T978	agent	by,conferred
R3890	T6768	T6765	prep	in,induction
R3891	T6769	T6770	compound	mutant,embryos
R3892	T6770	T6768	pobj	embryos,in
R3893	T6771	T6763	acomp	normal,is
R3894	T6772	T6763	punct	", ",is
R3895	T6773	T6763	advcl	implying,is
R3896	T6774	T6775	amod	proper,transport
R3897	T6775	T6773	dobj	transport,implying
R3898	T6776	T6775	cc	and,transport
R3899	T6777	T6775	conj	secretion,transport
R39	T247	T231	auxpass	is,required
R390	T983	T984	det	the,expansion
R3900	T6778	T6775	prep	of,transport
R3901	T6779	T6780	det	the,signals
R3902	T6780	T6778	pobj	signals,of
R3903	T6781	T6780	amod	appropriate,signals
R3904	T6782	T6780	amod	extraembryonic,signals
R3905	T6783	T6749	punct	.,impaired
R3906	T6785	T6786	advmod	However,expressed
R3907	T6787	T6786	punct	", ",expressed
R3908	T6788	T6786	nsubjpass	Nodal,expressed
R3909	T6789	T6788	punct	", ",Nodal
R391	T984	T982	pobj	expansion,by
R3910	T6790	T6788	conj	Fgf8,Nodal
R3911	T6791	T6790	cc	and,Fgf8
R3912	T6792	T6790	conj	Gsc,Fgf8
R3913	T6793	T6786	auxpass	are,expressed
R3914	T6794	T6786	advmod	ectopically,expressed
R3915	T6795	T6786	prep	in,expressed
R3916	T6796	T6797	det	the,endoderm
R3917	T6797	T6795	pobj	endoderm,in
R3918	T6798	T6797	amod	visceral,endoderm
R3919	T6799	T6797	prep	of,endoderm
R392	T985	T984	compound	polyglutamine,expansion
R3920	T6800	T6801	nmod	Hdhex4,embryos
R3921	T6801	T6799	pobj	embryos,of
R3922	T6802	T6800	punct	/,Hdhex4
R3923	T6803	T6800	nummod	5,Hdhex4
R3924	T6804	T6800	punct	/,Hdhex4
R3925	T6805	T6800	appos	Hdhex4,Hdhex4
R3926	T6806	T6805	punct	/,Hdhex4
R3927	T6807	T6805	nummod	5,Hdhex4
R3928	T6808	T6786	punct	.,expressed
R3929	T6810	T6811	preconj	Both,Nodal
R393	T986	T972	prep	to,leads
R3930	T6811	T6812	nsubjpass	Nodal,regulated
R3931	T6813	T6811	cc	and,Nodal
R3932	T6814	T6811	conj	Fgf8,Nodal
R3933	T6815	T6811	punct	", ",Nodal
R3934	T6816	T6817	amod	important,factors
R3935	T6817	T6811	appos	factors,Nodal
R3936	T6818	T6817	compound	growth,factors
R3937	T6819	T6817	acl	required,factors
R3938	T6820	T6819	prep	for,required
R3939	T6821	T6822	amod	normal,development
R394	T987	T988	det	the,loss
R3940	T6822	T6820	pobj	development,for
R3941	T6823	T6822	prep	of,development
R3942	T6824	T6825	det	the,epiblast
R3943	T6825	T6823	pobj	epiblast,of
R3944	T6826	T6812	punct	", ",regulated
R3945	T6827	T6812	auxpass	are,regulated
R3946	T6828	T6812	advmod	tightly,regulated
R3947	T6829	T6812	prep	during,regulated
R3948	T6830	T6829	pobj	gastrulation,during
R3949	T6831	T6812	punct	.,regulated
R395	T988	T986	pobj	loss,to
R3950	T6833	T6834	advmod	Therefore,contribute
R3951	T6835	T6834	punct	", ",contribute
R3952	T6836	T6834	nsubj	misexpression,contribute
R3953	T6837	T6836	prep	of,misexpression
R3954	T6838	T6837	pobj	either,of
R3955	T6839	T6838	cc	or,either
R3956	T6840	T6838	conj	both,either
R3957	T6841	T6838	prep	of,either
R3958	T6842	T6843	det	these,factors
R3959	T6843	T6841	pobj	factors,of
R396	T989	T988	compound	hallmark,loss
R3960	T6844	T6841	punct	", ",of
R3961	T6845	T6841	cc	or,of
R3962	T6846	T6841	conj	of,of
R3963	T6847	T6846	pobj	goosecoid,of
R3964	T6848	T6836	punct	", ",misexpression
R3965	T6849	T6836	prep	in,misexpression
R3966	T6850	T6851	det	the,endoderm
R3967	T6851	T6849	pobj	endoderm,in
R3968	T6852	T6851	amod	visceral,endoderm
R3969	T6853	T6834	aux	could,contribute
R397	T990	T988	prep	of,loss
R3970	T6854	T6834	prep	to,contribute
R3971	T6855	T6856	det	the,phenotype
R3972	T6856	T6854	pobj	phenotype,to
R3973	T6857	T6856	nmod	Hdhex4,phenotype
R3974	T6858	T6857	punct	/,Hdhex4
R3975	T6859	T6857	nummod	5,Hdhex4
R3976	T6860	T6857	punct	/,Hdhex4
R3977	T6861	T6857	appos	Hdhex4,Hdhex4
R3978	T6862	T6861	punct	/,Hdhex4
R3979	T6863	T6861	nummod	5,Hdhex4
R398	T991	T992	amod	striatal,neurons
R3980	T6864	T6856	compound	mutant,phenotype
R3981	T6865	T6834	punct	.,contribute
R3982	T6867	T6868	prep	In,is
R3983	T6869	T6867	pobj	addition,In
R3984	T6870	T6868	punct	", ",is
R3985	T6871	T6868	nsubj	it,is
R3986	T6872	T6868	acomp	possible,is
R3987	T6873	T6874	mark	that,affected
R3988	T6874	T6868	ccomp	affected,is
R3989	T6875	T6876	amod	other,signal
R399	T992	T990	pobj	neurons,of
R3990	T6876	T6874	nsubjpass	signal,affected
R3991	T6877	T6876	amod	extraembryonic,signal
R3992	T6878	T6876	punct	(,signal
R3993	T6879	T6876	nmod	s,signal
R3994	T6880	T6876	punct	),signal
R3995	T6881	T6882	dep	that,analyzed
R3996	T6882	T6876	relcl	analyzed,signal
R3997	T6883	T6882	nsubj	we,analyzed
R3998	T6884	T6882	aux	have,analyzed
R3999	T6885	T6882	neg	not,analyzed
R4	T207	T204	prep	of,Inactivation
R40	T248	T231	prep	in,required
R400	T993	T972	prep	in,leads
R4000	T6886	T6874	aux	may,affected
R4001	T6887	T6874	advmod	also,affected
R4002	T6888	T6874	auxpass	be,affected
R4003	T6889	T6874	agent	by,affected
R4004	T6890	T6891	det	the,lack
R4005	T6891	T6889	pobj	lack,by
R4006	T6892	T6891	prep	of,lack
R4007	T6893	T6894	compound	huntingtin,activity
R4008	T6894	T6892	pobj	activity,of
R4009	T6895	T6891	prep	in,lack
R401	T994	T993	pobj	HD,in
R4010	T6896	T6897	amod	extraembryonic,cells
R4011	T6897	T6895	pobj	cells,in
R4012	T6898	T6897	prep	in,cells
R4013	T6899	T6900	compound	mutant,embryos
R4014	T6900	T6898	pobj	embryos,in
R4015	T6901	T6868	punct	.,is
R4016	T6903	T6904	npadvmod	Huntingtin,deficient
R4017	T6904	T6905	amod	deficient,embryos
R4018	T6905	T6906	nsubj	embryos,fail
R4019	T6907	T6906	advmod	also,fail
R402	T995	T970	auxpass	is,known
R4020	T6908	T6909	aux	to,form
R4021	T6909	T6906	xcomp	form,fail
R4022	T6910	T6909	dobj	headfolds,form
R4023	T6911	T6909	punct	", ",form
R4024	T6912	T6909	cc	and,form
R4025	T6913	T6914	aux	to,undergo
R4026	T6914	T6909	conj	undergo,form
R4027	T6915	T6914	dobj	organogenesis,undergo
R4028	T6916	T6914	punct	", ",undergo
R4029	T6917	T6918	advmod	even,after
R403	T996	T970	neg	not,known
R4030	T6918	T6914	prep	after,undergo
R4031	T6919	T6918	pcomp	culturing,after
R4032	T6920	T6919	prep	in,culturing
R4033	T6921	T6922	npadvmod	nutrient,rich
R4034	T6922	T6923	amod	rich,media
R4035	T6923	T6920	pobj	media,in
R4036	T6924	T6906	punct	.,fail
R4037	T6926	T6927	det	The,absence
R4038	T6927	T6928	nsubj	absence,appear
R4039	T6929	T6927	prep	of,absence
R404	T997	T970	punct	.,known
R4040	T6930	T6931	compound	headfold,formation
R4041	T6931	T6929	pobj	formation,of
R4042	T6932	T6927	prep	in,absence
R4043	T6933	T6934	det	these,embryos
R4044	T6934	T6932	pobj	embryos,in
R4045	T6935	T6928	aux	does,appear
R4046	T6936	T6928	neg	not,appear
R4047	T6937	T6938	aux	to,be
R4048	T6938	T6928	xcomp	be,appear
R4049	T6939	T6940	det	a,result
R405	T999	T1000	advmod	However,cause
R4050	T6940	T6938	attr	result,be
R4051	T6941	T6940	prep	of,result
R4052	T6942	T6943	det	a,failure
R4053	T6943	T6941	pobj	failure,of
R4054	T6944	T6945	aux	to,induce
R4055	T6945	T6943	acl	induce,failure
R4056	T6946	T6945	dobj	neurectoderm,induce
R4057	T6947	T6943	cc	or,failure
R4058	T6948	T6949	det	a,failure
R4059	T6949	T6943	conj	failure,failure
R406	T1001	T1000	punct	", ",cause
R4060	T6950	T6951	aux	to,form
R4061	T6951	T6949	acl	form,failure
R4062	T6952	T6953	det	the,AVE
R4063	T6953	T6951	dobj	AVE,form
R4064	T6954	T6928	punct	", ",appear
R4065	T6955	T6956	mark	since,express
R4066	T6956	T6928	advcl	express,appear
R4067	T6957	T6958	compound	mutant,embryos
R4068	T6958	T6956	nsubj	embryos,express
R4069	T6959	T6956	dobj	markers,express
R407	T1002	T1003	compound	polyglutamine,expansions
R4070	T6960	T6961	amod	such,as
R4071	T6961	T6959	prep	as,markers
R4072	T6962	T6961	pobj	Otx2,as
R4073	T6963	T6962	punct	", ",Otx2
R4074	T6964	T6962	conj	Ddk1,Otx2
R4075	T6965	T6964	punct	", ",Ddk1
R4076	T6966	T6964	conj	Lefty1,Ddk1
R4077	T6967	T6966	cc	and,Lefty1
R4078	T6968	T6966	conj	Hesx1,Lefty1
R4079	T6969	T6928	punct	.,appear
R408	T1003	T1000	nsubj	expansions,cause
R4080	T6971	T6972	prep	In,is
R4081	T6973	T6971	pobj	addition,In
R4082	T6974	T6972	punct	", ",is
R4083	T6975	T6976	mark	since,required
R4084	T6976	T6972	advcl	required,is
R4085	T6977	T6978	compound	node,formation
R4086	T6978	T6976	nsubjpass	formation,required
R4087	T6979	T6976	auxpass	is,required
R4088	T6980	T6976	neg	not,required
R4089	T6981	T6976	prep	for,required
R409	T1004	T1003	prep	in,expansions
R4090	T6982	T6983	amod	neural,induction
R4091	T6983	T6981	pobj	induction,for
R4092	T6984	T6985	punct	[,44
R4093	T6985	T6976	parataxis	44,required
R4094	T6986	T6987	punct	-,46
R4095	T6987	T6985	prep	46,44
R4096	T6988	T6985	punct	],44
R4097	T6989	T6972	punct	", ",is
R4098	T6990	T6991	det	the,failure
R4099	T6991	T6972	nsubj	failure,is
R41	T249	T250	amod	extraembryonic,tissues
R410	T1005	T1006	amod	unrelated,proteins
R4100	T6992	T6993	aux	to,form
R4101	T6993	T6991	acl	form,failure
R4102	T6994	T6995	det	a,node
R4103	T6995	T6993	dobj	node,form
R4104	T6996	T6993	prep	in,form
R4105	T6997	T6998	npadvmod	huntingtin,deficient
R4106	T6998	T6999	amod	deficient,embryos
R4107	T6999	T6996	pobj	embryos,in
R4108	T7000	T6972	advmod	also,is
R4109	T7001	T6972	acomp	unlikely,is
R411	T1006	T1004	pobj	proteins,in
R4110	T7002	T7003	aux	to,explain
R4111	T7003	T7001	xcomp	explain,unlikely
R4112	T7004	T7005	det	the,lack
R4113	T7005	T7003	dobj	lack,explain
R4114	T7006	T7005	prep	of,lack
R4115	T7007	T7006	pobj	headfolds,of
R4116	T7008	T6972	punct	.,is
R4117	T7010	T7011	det	The,reduction
R4118	T7011	T7013	nsubj	reduction,explain
R4119	T7012	T7011	amod	apparent,reduction
R412	T1007	T1008	dep	that,target
R4120	T7014	T7011	prep	of,reduction
R4121	T7015	T7016	amod	paraxial,mesoderm
R4122	T7016	T7014	pobj	mesoderm,of
R4123	T7017	T7011	prep	in,reduction
R4124	T7018	T7019	nmod	Hdhex4,embryos
R4125	T7019	T7017	pobj	embryos,in
R4126	T7020	T7018	punct	/,Hdhex4
R4127	T7021	T7018	nummod	5,Hdhex4
R4128	T7022	T7018	punct	/,Hdhex4
R4129	T7023	T7018	appos	Hdhex4,Hdhex4
R413	T1008	T1006	relcl	target,proteins
R4130	T7024	T7023	punct	/,Hdhex4
R4131	T7025	T7023	nummod	5,Hdhex4
R4132	T7026	T7013	aux	could,explain
R4133	T7027	T7028	det	the,lack
R4134	T7028	T7013	dobj	lack,explain
R4135	T7029	T7028	prep	of,lack
R4136	T7030	T7029	pobj	headfolds,of
R4137	T7031	T7032	mark	since,is
R4138	T7032	T7013	advcl	is,explain
R4139	T7033	T7034	amod	paraxial,mesoderm
R414	T1009	T1010	amod	distinct,populations
R4140	T7034	T7032	nsubj	mesoderm,is
R4141	T7035	T7032	acomp	important,is
R4142	T7036	T7035	prep	for,important
R4143	T7037	T7038	det	the,development
R4144	T7038	T7036	pobj	development,for
R4145	T7039	T7038	amod	full,development
R4146	T7040	T7038	prep	of,development
R4147	T7041	T7040	pobj	neuroectoderm,of
R4148	T7042	T7041	punct	", ",neuroectoderm
R4149	T7043	T7041	cc	and,neuroectoderm
R415	T1010	T1008	dobj	populations,target
R4150	T7044	T7045	advmod	consequently,headfolds
R4151	T7045	T7041	conj	headfolds,neuroectoderm
R4152	T7046	T7045	punct	", ",headfolds
R4153	T7047	T7013	punct	.,explain
R4154	T7049	T7050	advmod	Alternatively,suggest
R4155	T7051	T7050	punct	", ",suggest
R4156	T7052	T7053	det	the,inability
R4157	T7053	T7050	nsubj	inability,suggest
R4158	T7054	T7055	aux	to,manifest
R4159	T7055	T7053	acl	manifest,inability
R416	T1011	T1010	amod	neuronal,populations
R4160	T7056	T7055	dobj	headfolds,manifest
R4161	T7057	T7050	aux	could,suggest
R4162	T7058	T7059	mark	that,required
R4163	T7059	T7050	ccomp	required,suggest
R4164	T7060	T7059	nsubjpass	huntingtin,required
R4165	T7061	T7059	auxpass	is,required
R4166	T7062	T7059	prep	at,required
R4167	T7063	T7064	det	a,stage
R4168	T7064	T7062	pobj	stage,at
R4169	T7065	T7066	advmod	very,early
R417	T1012	T1010	compound	cell,populations
R4170	T7066	T7064	amod	early,stage
R4171	T7067	T7059	prep	for,required
R4172	T7068	T7069	amod	normal,development
R4173	T7069	T7067	pobj	development,for
R4174	T7070	T7069	compound	CNS,development
R4175	T7071	T7050	punct	.,suggest
R4176	T7073	T7074	det	This,conclusion
R4177	T7074	T7075	nsubj	conclusion,is
R4178	T7076	T7075	acomp	consistent,is
R4179	T7077	T7076	prep	with,consistent
R418	T1013	T1014	amod	distinct,disorders
R4180	T7078	T7079	det	the,finding
R4181	T7079	T7077	pobj	finding,with
R4182	T7080	T7081	mark	that,lead
R4183	T7081	T7079	acl	lead,finding
R4184	T7082	T7083	advmod	severely,reduced
R4185	T7083	T7084	amod	reduced,levels
R4186	T7084	T7081	nsubj	levels,lead
R4187	T7085	T7084	prep	of,levels
R4188	T7086	T7085	pobj	huntingtin,of
R4189	T7087	T7084	punct	", ",levels
R419	T1014	T1000	dobj	disorders,cause
R4190	T7088	T7084	prep	from,levels
R4191	T7089	T7090	det	a,allele
R4192	T7090	T7088	pobj	allele,from
R4193	T7091	T7090	amod	hypomorphic,allele
R4194	T7092	T7090	compound	Hdh,allele
R4195	T7093	T7081	punct	", ",lead
R4196	T7094	T7081	prep	to,lead
R4197	T7095	T7096	amod	abnormal,brains
R4198	T7096	T7094	pobj	brains,to
R4199	T7097	T7081	advmod	later,lead
R42	T250	T248	pobj	tissues,in
R420	T1015	T1014	nmod	',disorders
R4200	T7098	T7097	prep	in,later
R4201	T7099	T7100	amod	embryonic,development
R4202	T7100	T7098	pobj	development,in
R4203	T7101	T7102	punct	[,15
R4204	T7102	T7081	parataxis	15,lead
R4205	T7103	T7102	punct	],15
R4206	T7104	T7075	punct	.,is
R4207	T7106	T7107	det	The,features
R4208	T7107	T7109	nsubj	features,are
R4209	T7108	T7107	amod	cardinal,features
R421	T1016	T1014	nmod	polyglutamine,disorders
R4210	T7110	T7107	prep	of,features
R4211	T7111	T7112	amod	complete,inactivation
R4212	T7112	T7110	pobj	inactivation,of
R4213	T7113	T7112	compound	Hdh,inactivation
R4214	T7114	T7115	dep	that,observe
R4215	T7115	T7107	relcl	observe,features
R4216	T7116	T7115	nsubj	we,observe
R4217	T7117	T7109	acomp	similar,are
R4218	T7118	T7117	prep	to,similar
R4219	T7119	T7120	det	the,phenotypes
R422	T1017	T1014	punct	',disorders
R4220	T7120	T7118	pobj	phenotypes,to
R4221	T7121	T7122	dep	that,stem
R4222	T7122	T7120	relcl	stem,phenotypes
R4223	T7123	T7122	prep	from,stem
R4224	T7124	T7125	det	the,inactivation
R4225	T7125	T7123	pobj	inactivation,from
R4226	T7126	T7125	amod	complete,inactivation
R4227	T7127	T7125	prep	of,inactivation
R4228	T7128	T7129	det	the,gene
R4229	T7129	T7127	pobj	gene,of
R423	T1018	T1014	amod	neurodegenerative,disorders
R4230	T7130	T7131	compound	Polycomb,group
R4231	T7131	T7129	compound	group,gene
R4232	T7132	T7129	punct	(,gene
R4233	T7133	T7134	compound	Pc,g
R4234	T7134	T7129	appos	g,gene
R4235	T7135	T7134	punct	-,g
R4236	T7136	T7129	punct	),gene
R4237	T7137	T7129	appos	Eed,gene
R4238	T7138	T7137	punct	(,Eed
R4239	T7139	T7140	amod	embryonic,development
R424	T1019	T1000	punct	.,cause
R4240	T7140	T7137	appos	development,Eed
R4241	T7141	T7140	compound	ectoderm,development
R4242	T7142	T7109	punct	),are
R4243	T7143	T7109	punct	.,are
R4244	T7145	T7146	advmod	Indeed,leads
R4245	T7147	T7146	punct	", ",leads
R4246	T7148	T7149	amod	complete,deficiency
R4247	T7149	T7146	nsubj	deficiency,leads
R4248	T7150	T7149	prep	for,deficiency
R4249	T7151	T7152	preconj	either,huntingtin
R425	T1021	T1022	det	This,observation
R4250	T7152	T7150	pobj	huntingtin,for
R4251	T7153	T7152	cc	or,huntingtin
R4252	T7154	T7155	det	the,protein
R4253	T7155	T7152	conj	protein,huntingtin
R4254	T7156	T7155	compound	eed,protein
R4255	T7157	T7146	prep	to,leads
R4256	T7158	T7159	amod	abnormal,development
R4257	T7159	T7157	pobj	development,to
R4258	T7160	T7159	compound	streak,development
R4259	T7161	T7159	punct	", ",development
R426	T1022	T1023	nsubj	observation,suggests
R4260	T7162	T7159	appos	lack,development
R4261	T7163	T7162	prep	of,lack
R4262	T7164	T7165	compound	headfold,formation
R4263	T7165	T7163	pobj	formation,of
R4264	T7166	T7159	punct	", ",development
R4265	T7167	T7168	amod	ectopic,T
R4266	T7168	T7159	appos	T,development
R4267	T7169	T7159	punct	", ",development
R4268	T7170	T7159	appos	Evx1,development
R4269	T7171	T7170	cc	and,Evx1
R427	T1024	T1023	advmod	strongly,suggests
R4270	T7172	T7170	conj	Nodal,Evx1
R4271	T7173	T7170	appos	expression,Evx1
R4272	T7174	T7173	cc	and,expression
R4273	T7175	T7173	conj	disruption,expression
R4274	T7176	T7170	prep	of,Evx1
R4275	T7177	T7178	amod	anterior,mesoderm
R4276	T7178	T7181	compound	mesoderm,production
R4277	T7179	T7178	amod	primitive,mesoderm
R4278	T7180	T7178	compound	streak,mesoderm
R4279	T7181	T7176	pobj	production,of
R428	T1025	T1026	mark	that,determined
R4280	T7182	T7183	punct	[,47
R4281	T7183	T7159	parataxis	47,development
R4282	T7184	T7183	punct	],47
R4283	T7185	T7146	punct	.,leads
R4284	T7187	T7188	advmod	Interestingly,required
R4285	T7189	T7188	punct	", ",required
R4286	T7190	T7191	compound	Eed,protein
R4287	T7191	T7188	nsubjpass	protein,required
R4288	T7192	T7188	auxpass	is,required
R4289	T7193	T7188	advmod	also,required
R429	T1026	T1023	ccomp	determined,suggests
R4290	T7194	T7188	prep	for,required
R4291	T7195	T7196	amod	proper,development
R4292	T7196	T7194	pobj	development,for
R4293	T7197	T7196	compound	trophoblast,development
R4294	T7198	T7196	cc	and,development
R4295	T7199	T7200	amod	normal,maintenance
R4296	T7200	T7196	conj	maintenance,development
R4297	T7201	T7200	prep	of,maintenance
R4298	T7202	T7203	amod	imprinted,inactivation
R4299	T7203	T7201	pobj	inactivation,of
R43	T251	T231	prep	for,required
R430	T1027	T1028	det	the,specificity
R4300	T7204	T7203	compound	X,inactivation
R4301	T7205	T7203	punct	-,inactivation
R4302	T7206	T7203	cc	and,inactivation
R4303	T7207	T7208	amod	genomic,imprinting
R4304	T7208	T7203	conj	imprinting,inactivation
R4305	T7209	T7210	punct	[,47
R4306	T7210	T7188	parataxis	47,required
R4307	T7211	T7212	punct	-,49
R4308	T7212	T7210	prep	49,47
R4309	T7213	T7210	punct	],47
R431	T1028	T1026	nsubjpass	specificity,determined
R4310	T7214	T7188	punct	", ",required
R4311	T7215	T7188	advcl	suggesting,required
R4312	T7216	T7217	mark	that,warrant
R4313	T7217	T7215	ccomp	warrant,suggesting
R4314	T7218	T7219	det	these,activities
R4315	T7219	T7217	nsubj	activities,warrant
R4316	T7220	T7217	dobj	investigation,warrant
R4317	T7221	T7217	prep	in,warrant
R4318	T7222	T7223	npadvmod	huntingtin,deficient
R4319	T7223	T7224	amod	deficient,embryos
R432	T1029	T1030	amod	striatal,cell
R4320	T7224	T7221	pobj	embryos,in
R4321	T7225	T7188	punct	.,required
R4322	T7227	T7228	advmod	Thus,provide
R4323	T7229	T7228	punct	", ",provide
R4324	T7230	T7231	poss	our,observations
R4325	T7231	T7228	nsubj	observations,provide
R4326	T7232	T7233	amod	unexpected,points
R4327	T7233	T7228	dobj	points,provide
R4328	T7234	T7233	amod	starting,points
R4329	T7235	T7233	punct	-,points
R433	T1030	T1028	compound	cell,specificity
R4330	T7236	T7228	prep	in,provide
R4331	T7237	T7238	det	the,search
R4332	T7238	T7236	pobj	search,in
R4333	T7239	T7238	prep	for,search
R4334	T7240	T7241	poss	huntingtin,activities
R4335	T7241	T7239	pobj	activities,for
R4336	T7242	T7240	case	's,huntingtin
R4337	T7243	T7241	amod	precise,activities
R4338	T7244	T7241	amod	molecular,activities
R4339	T7245	T7238	punct	", ",search
R434	T1031	T1028	prep	of,specificity
R4340	T7246	T7247	dep	which,began
R4341	T7247	T7238	relcl	began,search
R4342	T7248	T7247	prep	with,began
R4343	T7249	T7250	det	the,discovery
R4344	T7250	T7248	pobj	discovery,with
R4345	T7251	T7252	mark	that,hosts
R4346	T7252	T7250	acl	hosts,discovery
R4347	T7253	T7254	det	this,protein
R4348	T7254	T7252	nsubj	protein,hosts
R4349	T7255	T7256	compound	HEAT,domain
R435	T1032	T1033	det	the,expansion
R4350	T7256	T7254	compound	domain,protein
R4351	T7257	T7258	det	the,property
R4352	T7258	T7252	dobj	property,hosts
R4353	T7259	T7258	amod	dominant,property
R4354	T7260	T7258	compound	polyglutamine,property
R4355	T7261	T7262	dep	that,is
R4356	T7262	T7258	relcl	is,property
R4357	T7263	T7264	det	the,basis
R4358	T7264	T7262	attr	basis,is
R4359	T7265	T7264	amod	fundamental,basis
R436	T1033	T1031	pobj	expansion,of
R4360	T7266	T7264	prep	of,basis
R4361	T7267	T7268	compound	HD,pathogenesis
R4362	T7268	T7266	pobj	pathogenesis,of
R4363	T7269	T7228	punct	.,provide
R4364	T7271	T7272	prep	In,affects
R4365	T7273	T7274	compound	HD,patients
R4366	T7274	T7271	pobj	patients,In
R4367	T7275	T7271	cc	and,In
R4368	T7276	T7271	conj	in,In
R4369	T7277	T7278	amod	accurate,replicas
R437	T1034	T1033	compound	polyglutamine,expansion
R4370	T7278	T7276	pobj	replicas,in
R4371	T7279	T7278	amod	genetic,replicas
R4372	T7280	T7278	punct	", ",replicas
R4373	T7281	T7282	nmod	HD,mice
R4374	T7282	T7278	appos	mice,replicas
R4375	T7283	T7282	nmod	CAG,mice
R4376	T7284	T7282	amod	knock,mice
R4377	T7285	T7284	punct	-,knock
R4378	T7286	T7284	prt	in,knock
R4379	T7287	T7272	punct	", ",affects
R438	T1035	T1028	prep	in,specificity
R4380	T7288	T7289	det	the,mutation
R4381	T7289	T7272	nsubj	mutation,affects
R4382	T7290	T7289	amod	dominant,mutation
R4383	T7291	T7272	advmod	specifically,affects
R4384	T7292	T7293	det	the,population
R4385	T7293	T7272	dobj	population,affects
R4386	T7294	T7293	amod	major,population
R4387	T7295	T7293	prep	of,population
R4388	T7296	T7295	pobj	neurons,of
R4389	T7297	T7296	prep	in,neurons
R439	T1036	T1037	det	the,context
R4390	T7298	T7299	det	the,striatum
R4391	T7299	T7297	pobj	striatum,in
R4392	T7300	T7272	punct	", ",affects
R4393	T7301	T7272	prep	without,affects
R4394	T7302	T7301	pcomp	impairing,without
R4395	T7303	T7304	poss	huntingtin,activities
R4396	T7304	T7302	dobj	activities,impairing
R4397	T7305	T7303	case	's,huntingtin
R4398	T7306	T7304	amod	essential,activities
R4399	T7307	T7304	prep	in,activities
R44	T252	T253	amod	proper,gastrulation
R440	T1037	T1035	pobj	context,in
R4400	T7308	T7309	amod	embryonic,development
R4401	T7309	T7307	pobj	development,in
R4402	T7310	T7311	punct	[,50
R4403	T7311	T7272	parataxis	50,affects
R4404	T7312	T7313	punct	-,53
R4405	T7313	T7311	prep	53,50
R4406	T7314	T7311	punct	],50
R4407	T7315	T7272	punct	.,affects
R4408	T7317	T7318	advmod	Indeed,make
R4409	T7319	T7318	punct	", ",make
R441	T1038	T1037	prep	of,context
R4410	T7320	T7321	amod	homozygous,patients
R4411	T7321	T7318	nsubj	patients,make
R4412	T7322	T7321	compound	HD,patients
R4413	T7323	T7324	det	no,huntingtin
R4414	T7324	T7318	dobj	huntingtin,make
R4415	T7325	T7326	amod	wild,type
R4416	T7326	T7324	compound	type,huntingtin
R4417	T7327	T7326	punct	-,type
R4418	T7328	T7318	punct	", ",make
R4419	T7329	T7318	cc	and,make
R442	T1039	T1038	pobj	huntingtin,of
R4420	T7330	T7318	punct	", ",make
R4421	T7331	T7332	prep	in,rescue
R4422	T7332	T7318	conj	rescue,make
R4423	T7333	T7334	det	the,mouse
R4424	T7334	T7331	pobj	mouse,in
R4425	T7335	T7332	punct	", ",rescue
R4426	T7336	T7337	det	a,allele
R4427	T7337	T7341	poss	allele,worth
R4428	T7338	T7337	amod	single,allele
R4429	T7339	T7337	compound	mutant,allele
R443	T1040	T1026	aux	must,determined
R4430	T7340	T7337	compound	Hdh,allele
R4431	T7341	T7332	nsubj	worth,rescue
R4432	T7342	T7337	case	's,allele
R4433	T7343	T7341	prep	of,worth
R4434	T7344	T7345	compound	mutant,huntingtin
R4435	T7345	T7343	pobj	huntingtin,of
R4436	T7346	T7332	aux	can,rescue
R4437	T7347	T7332	advmod	fully,rescue
R4438	T7348	T7349	nmod	huntingtin,deficiency
R4439	T7349	T7350	nmod	deficiency,phenotypes
R444	T1041	T1026	auxpass	be,determined
R4440	T7350	T7332	dobj	phenotypes,rescue
R4441	T7351	T7350	amod	embryonic,phenotypes
R4442	T7352	T7353	punct	[,51
R4443	T7353	T7332	parataxis	51,rescue
R4444	T7373	T7374	det	the,activity
R4445	T7374	T7372	dobj	activity,delineating
R4446	T7354	T7353	nummod	15,51
R4447	T7375	T7374	compound	huntingtin,activity
R4448	T7376	T7377	dep	that,explain
R4449	T7355	T7353	punct	",",51
R445	T1042	T1026	agent	by,determined
R4450	T7377	T7374	relcl	explain,activity
R4451	T7378	T7377	aux	may,explain
R4452	T7379	T7380	det	the,specificity
R4453	T7356	T7353	punct	],51
R4454	T7380	T7377	dobj	specificity,explain
R4455	T7357	T7332	punct	.,rescue
R4456	T7381	T7380	amod	striatal,specificity
R4457	T7382	T7380	compound	cell,specificity
R4458	T7383	T7380	prep	of,specificity
R4459	T7359	T7360	det	The,quest
R446	T1043	T1044	det	some,aspect
R4460	T7384	T7385	det	the,version
R4461	T7385	T7383	pobj	version,of
R4462	T7386	T7385	compound	polyglutamine,version
R4463	T7360	T7361	nsubjpass	quest,aimed
R4464	T7387	T7385	compound	mutant,version
R4465	T7388	T7385	prep	of,version
R4466	T7389	T7388	pobj	huntingtin,of
R4467	T7362	T7363	aux	to,understand
R4468	T7390	T7361	punct	.,aimed
R4469	T7392	T7393	nummod	One,hypothesis
R447	T1044	T1042	pobj	aspect,by
R4470	T7393	T7394	nsubj	hypothesis,is
R4471	T7363	T7360	acl	understand,quest
R4472	T7395	T7396	mark	that,involved
R4473	T7364	T7365	det	the,mechanism
R4474	T7396	T7394	ccomp	involved,is
R4475	T7397	T7396	nsubjpass	huntingtin,involved
R4476	T7398	T7396	auxpass	is,involved
R4477	T7365	T7363	dobj	mechanism,understand
R4478	T7399	T7396	advmod	normally,involved
R4479	T7400	T7396	prep	in,involved
R448	T1045	T1044	prep	of,aspect
R4480	T7401	T7402	compound	gene,transcription
R4481	T7366	T7365	compound	HD,mechanism
R4482	T7402	T7400	pobj	transcription,in
R4483	T7403	T7396	punct	", ",involved
R4484	T7367	T7361	punct	", ",aimed
R4485	T7404	T7405	mark	as,proposed
R4486	T7368	T7361	advmod	therefore,aimed
R4487	T7405	T7396	advcl	proposed,involved
R4488	T7406	T7405	prep	for,proposed
R4489	T7407	T7408	compound	NRSF,REST
R449	T1046	T1047	poss	huntingtin,structure
R4490	T7408	T7410	npadvmod	REST,mediated
R4491	T7409	T7408	punct	/,REST
R4492	T7369	T7361	punct	", ",aimed
R4493	T7410	T7411	amod	mediated,expression
R4494	T7411	T7406	pobj	expression,for
R4495	T7412	T7411	compound	BDNF,expression
R4496	T7370	T7361	auxpass	is,aimed
R4497	T7413	T7414	punct	[,54
R4498	T7414	T7405	parataxis	54,proposed
R4499	T7415	T7414	punct	],54
R45	T253	T251	pobj	gastrulation,for
R450	T1047	T1045	pobj	structure,of
R4500	T7416	T7394	punct	.,is
R4501	T7371	T7361	prep	at,aimed
R4502	T7418	T7419	advmod	Now,provides
R4503	T7372	T7371	pcomp	delineating,at
R4504	T7420	T7419	punct	", ",provides
R4505	T7421	T7422	poss	our,finding
R4506	T7422	T7419	nsubj	finding,provides
R4507	T7423	T7424	mark	that,be
R4508	T7424	T7422	acl	be,finding
R4509	T7425	T7424	nsubj	huntingtin,be
R451	T1048	T1046	case	's,huntingtin
R4510	T7426	T7424	aux	can,be
R4511	T7427	T7428	advmod	absolutely,necessary
R4512	T7428	T7424	acomp	necessary,be
R4513	T7429	T7428	prep	for,necessary
R4514	T7430	T7431	det	the,regulation
R4515	T7431	T7429	pobj	regulation,for
R4516	T7432	T7431	amod	appropriate,regulation
R4517	T7433	T7431	prep	of,regulation
R4518	T7434	T7433	pobj	genes,of
R4519	T7435	T7434	prep	with,genes
R452	T1049	T1047	punct	", ",structure
R4520	T7436	T7437	amod	dynamic,patterns
R4521	T7437	T7435	pobj	patterns,with
R4522	T7438	T7437	compound	expression,patterns
R4523	T7439	T7440	advmod	in,vivo
R4524	T7440	T7437	advmod	vivo,patterns
R4525	T7441	T7419	punct	", ",provides
R4526	T7442	T7443	det	a,reason
R4527	T7443	T7419	dobj	reason,provides
R4528	T7444	T7443	amod	compelling,reason
R4529	T7445	T7446	aux	to,elucidate
R453	T1050	T1051	amod	subcellular,location
R4530	T7446	T7443	acl	elucidate,reason
R4531	T7447	T7448	det	the,machinery
R4532	T7448	T7446	dobj	machinery,elucidate
R4533	T7449	T7448	amod	cellular,machinery
R4534	T7450	T7451	dep	that,is
R4535	T7451	T7448	relcl	is,machinery
R4536	T7452	T7451	acomp	necessary,is
R4537	T7453	T7452	prep	for,necessary
R4538	T7454	T7455	npadvmod	huntingtin,mediated
R4539	T7455	T7456	amod	mediated,regulation
R454	T1051	T1047	conj	location,structure
R4540	T7456	T7453	pobj	regulation,for
R4541	T7457	T7456	compound	gene,regulation
R4542	T7458	T7419	punct	.,provides
R4548	T7532	T7533	poss	Our,findings
R4549	T7533	T7534	nsubj	findings,indicate
R455	T1052	T1051	cc	or,location
R4550	T7535	T7536	mark	that,required
R4551	T7536	T7534	ccomp	required,indicate
R4552	T7537	T7536	nsubjpass	huntingtin,required
R4553	T7538	T7536	auxpass	is,required
R4554	T7539	T7536	prep	for,required
R4555	T7540	T7541	amod	proper,patterning
R4556	T7541	T7539	pobj	patterning,for
R4557	T7542	T7541	prep	of,patterning
R4558	T7543	T7544	det	the,epiblast
R4559	T7544	T7542	pobj	epiblast,of
R456	T1053	T1051	conj	activities,location
R4560	T7545	T7541	prep	during,patterning
R4561	T7546	T7547	amod	early,embryogenesis
R4562	T7547	T7545	pobj	embryogenesis,during
R4563	T7548	T7539	punct	", ",for
R4564	T7549	T7539	conj	for,for
R4565	T7550	T7551	amod	proper,formation
R4566	T7551	T7549	pobj	formation,for
R4567	T7552	T7553	amod	anterior,streak
R4568	T7553	T7551	nmod	streak,formation
R4569	T7554	T7553	cc	and,streak
R457	T1054	T1055	punct	[,2
R4570	T7555	T7553	conj	node,streak
R4571	T7556	T7551	punct	", ",formation
R4572	T7557	T7558	amod	primitive,streak
R4573	T7558	T7559	compound	streak,progression
R4574	T7559	T7551	appos	progression,formation
R4575	T7560	T7551	punct	", ",formation
R4576	T7561	T7562	amod	paraxial,mesoderm
R4577	T7562	T7563	nmod	mesoderm,formation
R4578	T7563	T7551	appos	formation,formation
R4579	T7564	T7562	cc	and,mesoderm
R458	T1055	T1026	parataxis	2,determined
R4580	T7565	T7566	compound	head,fold
R4581	T7566	T7562	conj	fold,mesoderm
R4582	T7567	T7549	punct	", ",for
R4583	T7568	T7569	advmod	as,as
R4584	T7569	T7549	cc	as,for
R4585	T7570	T7569	advmod	well,as
R4586	T7571	T7549	conj	for,for
R4587	T7572	T7573	det	the,restriction
R4588	T7573	T7571	pobj	restriction,for
R4589	T7574	T7573	amod	proper,restriction
R459	T1056	T1055	punct	],2
R4590	T7575	T7573	prep	of,restriction
R4591	T7576	T7577	advmod	transiently,expressed
R4592	T7577	T7578	amod	expressed,genes
R4593	T7578	T7575	pobj	genes,of
R4594	T7579	T7580	nmod	growth,factor
R4595	T7580	T7578	compound	factor,genes
R4596	T7581	T7579	cc	and,growth
R4597	T7582	T7579	conj	transcription,growth
R4598	T7583	T7534	punct	.,indicate
R4599	T7585	T7586	nsubj	Knowledge,facilitate
R46	T254	T231	punct	", ",required
R460	T1057	T1023	punct	.,suggests
R4600	T7587	T7585	prep	of,Knowledge
R4601	T7588	T7589	det	the,basis
R4602	T7589	T7587	pobj	basis,of
R4603	T7590	T7589	amod	molecular,basis
R4604	T7591	T7589	prep	of,basis
R4605	T7592	T7593	det	these,changes
R4606	T7593	T7591	pobj	changes,of
R4607	T7594	T7589	prep	in,basis
R4608	T7595	T7596	npadvmod	huntingtin,deficient
R4609	T7596	T7597	amod	deficient,embryos
R461	T1059	T1060	nsubjpass	Huntingtin,postulated
R4610	T7597	T7594	pobj	embryos,in
R4611	T7598	T7586	aux	should,facilitate
R4612	T7599	T7600	det	the,identification
R4613	T7600	T7586	dobj	identification,facilitate
R4614	T7601	T7600	prep	of,identification
R4615	T7602	T7603	det	the,pathways
R4616	T7603	T7601	pobj	pathways,of
R4617	T7604	T7603	amod	cellular,pathways
R4618	T7605	T7606	dep	that,are
R4619	T7606	T7603	relcl	are,pathways
R462	T1061	T1060	auxpass	is,postulated
R4620	T7607	T7606	acomp	dependent,are
R4621	T7608	T7607	prep	on,dependent
R4622	T7609	T7610	compound	huntingtin,activities
R4623	T7610	T7608	pobj	activities,on
R4624	T7611	T7586	punct	.,facilitate
R4625	T7613	T7614	nsubj	These,be
R4626	T7615	T7614	aux	will,be
R4627	T7616	T7614	acomp	important,be
R4628	T7617	T7616	prep	for,important
R4629	T7618	T7617	pcomp	implicating,for
R463	T1062	T1063	aux	to,function
R4630	T7619	T7618	dobj	candidates,implicating
R4631	T7620	T7621	aux	to,assessed
R4632	T7621	T7619	advcl	assessed,candidates
R4633	T7622	T7621	auxpass	be,assessed
R4634	T7623	T7618	prep	in,implicating
R4635	T7624	T7625	det	the,signals
R4636	T7625	T7623	pobj	signals,in
R4637	T7626	T7625	amod	extraembryonic,signals
R4638	T7627	T7628	dep	that,determine
R4639	T7628	T7625	relcl	determine,signals
R464	T1063	T1060	xcomp	function,postulated
R4640	T7629	T7630	amod	anterior,streak
R4641	T7630	T7631	compound	streak,progression
R4642	T7631	T7628	dobj	progression,determine
R4643	T7632	T7628	prep	in,determine
R4644	T7633	T7634	det	the,embryo
R4645	T7634	T7632	pobj	embryo,in
R4646	T7635	T7634	amod	developing,embryo
R4647	T7636	T7632	cc	and,in
R4648	T7637	T7632	conj	in,in
R4649	T7638	T7637	pcomp	delineating,in
R465	T1064	T1063	prep	as,function
R4650	T7639	T7640	det	the,activity
R4651	T7640	T7638	dobj	activity,delineating
R4652	T7641	T7640	amod	dominant,activity
R4653	T7642	T7640	prep	of,activity
R4654	T7643	T7644	det	the,tract
R4655	T7644	T7642	pobj	tract,of
R4656	T7645	T7644	compound	polyglutamine,tract
R4657	T7646	T7640	prep	in,activity
R4658	T7647	T7646	pobj	huntingtin,in
R4659	T7648	T7649	dep	that,determines
R466	T1065	T1066	det	a,scaffold
R4660	T7649	T7640	relcl	determines,activity
R4661	T7650	T7651	det	the,specificity
R4662	T7651	T7649	dobj	specificity,determines
R4663	T7652	T7651	amod	striatal,specificity
R4664	T7653	T7651	prep	of,specificity
R4665	T7654	T7653	pobj	HD,of
R4666	T7655	T7614	punct	.,be
R4667	T7726	T7725	cc	and,Mice
R4668	T7727	T7725	conj	genotyping,Mice
R4669	T7729	T7730	det	The,mice
R467	T1066	T1064	pobj	scaffold,as
R4670	T7730	T7734	nsubjpass	mice,described
R4671	T7731	T7730	nmod	Hdhex4,mice
R4672	T7732	T7731	punct	/,Hdhex4
R4673	T7733	T7731	nummod	5,Hdhex4
R4674	T7735	T7730	acl	carrying,mice
R4675	T7736	T7737	det	a,mutation
R4676	T7737	T7735	dobj	mutation,carrying
R4677	T7738	T7739	compound	pGKneo,replacement
R4678	T7739	T7742	npadvmod	replacement,inactivating
R4679	T7740	T7739	compound	insertion,replacement
R468	T1067	T1066	amod	flexible,scaffold
R4680	T7741	T7739	punct	/,replacement
R4681	T7742	T7737	amod	inactivating,mutation
R4682	T7743	T7735	prep	in,carrying
R4683	T7744	T7745	det	the,homologue
R4684	T7745	T7743	pobj	homologue,in
R4685	T7746	T7745	compound	mouse,homologue
R4686	T7747	T7745	compound	HD,homologue
R4687	T7748	T7745	compound	gene,homologue
R4688	T7749	T7734	aux	have,described
R4689	T7750	T7734	auxpass	been,described
R469	T1068	T1069	punct	~,350
R4690	T7751	T7734	advmod	previously,described
R4691	T7752	T7753	punct	[,10
R4692	T7753	T7734	parataxis	10,described
R4693	T7754	T7753	punct	],10
R4694	T7755	T7734	punct	.,described
R4695	T7757	T7758	det	The,experiments
R4696	T7758	T7759	nsubjpass	experiments,conducted
R4697	T7760	T7759	auxpass	were,conducted
R4698	T7761	T7759	prep	in,conducted
R4699	T7762	T7761	pobj	accordance,in
R47	T255	T231	advcl	implicating,required
R470	T1069	T1070	nummod	350,kDa
R4700	T7763	T7762	prep	with,accordance
R4701	T7764	T7765	det	an,protocol
R4702	T7765	T7763	pobj	protocol,with
R4703	T7766	T7767	npadvmod	IACUC,approved
R4704	T7767	T7765	amod	approved,protocol
R4705	T7768	T7759	punct	", ",conducted
R4706	T7769	T7759	prep	through,conducted
R4707	T7770	T7771	det	the,Subcommittee
R4708	T7771	T7769	pobj	Subcommittee,through
R4709	T7772	T7771	compound	MGH,Subcommittee
R471	T1070	T1066	compound	kDa,scaffold
R4710	T7773	T7771	prep	on,Subcommittee
R4711	T7774	T7775	compound	Animal,Research
R4712	T7775	T7773	pobj	Research,on
R4713	T7776	T7759	punct	.,conducted
R4714	T7778	T7779	nmod	Mutant,Hdhex4
R4715	T7779	T7780	nmod	Hdhex4,littermates
R4716	T7780	T7789	nsubjpass	littermates,obtained
R4717	T7781	T7779	punct	/,Hdhex4
R4718	T7782	T7779	nummod	5,Hdhex4
R4719	T7783	T7779	punct	/,Hdhex4
R472	T1071	T1072	compound	HEAT,domain
R4720	T7784	T7779	appos	Hdhex4,Hdhex4
R4721	T7785	T7784	punct	/,Hdhex4
R4722	T7786	T7784	nummod	5,Hdhex4
R4723	T7787	T7779	cc	and,Hdhex4
R4724	T7788	T7779	conj	normal,Hdhex4
R4725	T7790	T7789	auxpass	were,obtained
R4726	T7791	T7789	prep	in,obtained
R4727	T7792	T7793	amod	timed,pregnancies
R4728	T7793	T7791	pobj	pregnancies,in
R4729	T7794	T7789	prep	from,obtained
R473	T1072	T1066	compound	domain,scaffold
R4730	T7795	T7794	pobj	mating,from
R4731	T7796	T7795	prep	of,mating
R4732	T7797	T7798	nmod	Hdhex4,heterozygotes
R4733	T7798	T7796	pobj	heterozygotes,of
R4734	T7799	T7797	punct	/,Hdhex4
R4735	T7800	T7797	nummod	5,Hdhex4
R4736	T7801	T7797	punct	/,Hdhex4
R4737	T7802	T7797	appos	Hdh,Hdhex4
R4738	T7803	T7802	punct	+,Hdh
R4739	T7804	T7798	punct	", ",heterozygotes
R474	T1073	T1074	dep	that,facilitate
R4740	T7805	T7798	acl	genotyped,heterozygotes
R4741	T7806	T7805	prep	by,genotyped
R4742	T7807	T7808	compound	PCR,assay
R4743	T7808	T7806	pobj	assay,by
R4744	T7809	T7789	punct	", ",obtained
R4745	T7810	T7811	mark	as,described
R4746	T7811	T7789	advcl	described,obtained
R4747	T7812	T7813	punct	[,10
R4748	T7813	T7811	parataxis	10,described
R4749	T7814	T7813	punct	],10
R475	T1074	T1066	relcl	facilitate,scaffold
R4750	T7815	T7789	punct	.,obtained
R4751	T7817	T7818	det	The,day
R4752	T7818	T7819	nsubjpass	day,taken
R4753	T7820	T7818	prep	of,day
R4754	T7821	T7820	pobj	plug,of
R4755	T7822	T7819	auxpass	was,taken
R4756	T7823	T7824	aux	to,be
R4757	T7824	T7819	xcomp	be,taken
R4758	T7825	T7824	attr	E0.5,be
R4759	T7826	T7819	punct	.,taken
R476	T1075	T1074	aux	may,facilitate
R4760	T7828	T7829	nsubjpass	Embryos,pooled
R4761	T7830	T7831	dep	that,were
R4762	T7831	T7828	relcl	were,Embryos
R4763	T7832	T7833	advmod	morphologically,normal
R4764	T7833	T7831	acomp	normal,were
R4765	T7834	T7829	auxpass	were,pooled
R4766	T7835	T7836	advmod	separately,from
R4767	T7836	T7829	prep	from,pooled
R4768	T7837	T7838	advmod	morphologically,mutant
R4769	T7838	T7839	compound	mutant,embryos
R477	T1076	T1077	det	the,assembly
R4770	T7839	T7836	pobj	embryos,from
R4771	T7840	T7829	prep	for,pooled
R4772	T7841	T7840	pobj	analysis,for
R4773	T7842	T7829	punct	.,pooled
R4774	T7844	T7845	compound	Nodal,expression
R4775	T7845	T7846	nsubjpass	expression,determined
R4776	T7847	T7846	auxpass	was,determined
R4777	T7848	T7846	prep	in,determined
R4778	T7849	T7848	pobj	embryos,in
R4779	T7850	T7849	prep	from,embryos
R478	T1077	T1074	dobj	assembly,facilitate
R4780	T7851	T7850	pobj	matings,from
R4781	T7852	T7851	prep	of,matings
R4782	T7853	T7854	nmod	Hdhex4,heterozygotes
R4783	T7854	T7852	pobj	heterozygotes,of
R4784	T7855	T7853	punct	/,Hdhex4
R4785	T7856	T7853	nummod	5,Hdhex4
R4786	T7857	T7853	punct	/,Hdhex4
R4787	T7858	T7853	appos	Hdh,Hdhex4
R4788	T7859	T7858	punct	+,Hdh
R4789	T7860	T7853	punct	;,Hdhex4
R479	T1078	T1077	cc	and,assembly
R4790	T7861	T7862	compound	NdllacZ,Ndl
R4791	T7862	T7853	appos	Ndl,Hdhex4
R4792	T7863	T7862	punct	/,Ndl
R4793	T7864	T7862	punct	+,Ndl
R4794	T7865	T7854	compound	compound,heterozygotes
R4795	T7866	T7854	acl	genotyped,heterozygotes
R4796	T7867	T7866	prep	by,genotyped
R4797	T7868	T7869	compound	PCR,assay
R4798	T7869	T7867	pobj	assay,by
R4799	T7870	T7871	mark	as,described
R48	T256	T257	poss	its,activities
R480	T1079	T1080	advmod	possibly,location
R4800	T7871	T7866	advcl	described,genotyped
R4801	T7872	T7871	prep	in,described
R4802	T7873	T7874	punct	[,29
R4803	T7874	T7872	pobj	29,in
R4804	T7875	T7874	punct	],29
R4805	T7876	T7846	punct	.,determined
R4806	T7923	T7924	amod	Whole,mount
R4807	T7924	T7925	nmod	mount,hybridization
R4808	T7926	T7924	cc	and,mount
R4809	T7927	T7924	conj	section,mount
R481	T1080	T1077	conj	location,assembly
R4810	T7928	T7929	advmod	in,situ
R4811	T7929	T7925	amod	situ,hybridization
R4812	T7930	T7925	cc	and,hybridization
R4813	T7931	T7932	compound	β,gal
R4814	T7932	T7934	compound	gal,staining
R4815	T7933	T7932	punct	-,gal
R4816	T7934	T7925	conj	staining,hybridization
R4817	T7936	T7937	prep	After,fixed
R4818	T7938	T7936	pobj	dissection,After
R4819	T7939	T7938	prep	in,dissection
R482	T1081	T1080	det	the,location
R4820	T7940	T7939	pobj	PBS,in
R4821	T7941	T7937	punct	", ",fixed
R4822	T7942	T7937	nsubjpass	embryos,fixed
R4823	T7943	T7937	auxpass	were,fixed
R4824	T7944	T7937	advmod	overnight,fixed
R4825	T7945	T7937	prep	in,fixed
R4826	T7946	T7947	nummod	4,%
R4827	T7947	T7948	compound	%,paraformaldehyde
R4828	T7948	T7945	pobj	paraformaldehyde,in
R4829	T7949	T7937	prep	at,fixed
R483	T1082	T1080	amod	subcellular,location
R4830	T7950	T7951	nummod	4,°C
R4831	T7951	T7949	pobj	°C,at
R4832	T7952	T7937	punct	.,fixed
R4833	T7954	T7955	prep	For,embedded
R4834	T7956	T7954	pobj	sections,For
R4835	T7957	T7955	punct	", ",embedded
R4836	T7958	T7955	nsubjpass	decidua,embedded
R4837	T7959	T7958	acl	fixed,decidua
R4838	T7960	T7959	prep	in,fixed
R4839	T7961	T7962	nummod	4,%
R484	T1083	T1077	prep	of,assembly
R4840	T7962	T7963	compound	%,paraformaldehyde
R4841	T7963	T7960	pobj	paraformaldehyde,in
R4842	T7964	T7955	punct	", ",embedded
R4843	T7965	T7955	auxpass	were,embedded
R4844	T7966	T7955	prep	in,embedded
R4845	T7967	T7966	pobj	paraffin,in
R4846	T7968	T7955	cc	and,embedded
R4847	T7969	T7955	conj	sectioned,embedded
R4848	T7970	T7969	prep	at,sectioned
R4849	T7971	T7972	nummod	7,microns
R485	T1084	T1085	amod	large,complexes
R4850	T7972	T7970	pobj	microns,at
R4851	T7973	T7955	punct	.,embedded
R4852	T7975	T7976	nmod	RNA,hybridizations
R4853	T7976	T7979	nsubjpass	hybridizations,performed
R4854	T7977	T7978	advmod	in,situ
R4855	T7978	T7976	amod	situ,hybridizations
R4856	T7980	T7979	auxpass	were,performed
R4857	T7981	T7982	mark	as,described
R4858	T7982	T7979	advcl	described,performed
R4859	T7983	T7982	advmod	previously,described
R486	T1085	T1083	pobj	complexes,of
R4860	T7984	T7985	punct	[,55
R4861	T7985	T7982	parataxis	55,described
R4862	T7986	T7985	punct	],55
R4863	T7987	T7979	punct	.,performed
R4864	T7989	T7990	compound	Nodal,lacZ
R4865	T7990	T7992	compound	lacZ,expression
R4866	T7991	T7990	punct	.,lacZ
R4867	T7992	T7993	nsubjpass	expression,assessed
R4868	T7994	T7993	auxpass	was,assessed
R4869	T7995	T7993	prep	by,assessed
R487	T1086	T1085	compound	protein,complexes
R4870	T7996	T7997	compound	β,galactosidase
R4871	T7997	T7999	compound	galactosidase,staining
R4872	T7998	T7997	punct	-,galactosidase
R4873	T7999	T7995	pobj	staining,by
R4874	T8000	T8001	mark	as,reported
R4875	T8001	T7993	advcl	reported,assessed
R4876	T8002	T8003	punct	[,29
R4877	T8003	T8001	parataxis	29,reported
R4878	T8004	T8003	punct	],29
R4879	T8005	T7993	punct	", ",assessed
R488	T1087	T1088	punct	[,3
R4880	T8006	T7993	prep	on,assessed
R4881	T8007	T8006	pobj	embryos,on
R4882	T8008	T8009	dep	post,fixed
R4883	T8009	T8007	acl	fixed,embryos
R4884	T8010	T8009	prep	in,fixed
R4885	T8011	T8012	nummod	4,%
R4886	T8012	T8013	compound	%,paraformaldehyde
R4887	T8013	T8010	pobj	paraformaldehyde,in
R4888	T8014	T7993	punct	.,assessed
R4889	T8016	T8017	nsubjpass	Embryos,mounted
R489	T1088	T1060	parataxis	3,postulated
R4890	T8018	T8017	auxpass	were,mounted
R4891	T8019	T8017	prep	in,mounted
R4892	T8020	T8021	nummod	80,%
R4893	T8021	T8022	compound	%,glycerol
R4894	T8022	T8019	pobj	glycerol,in
R4895	T8023	T8017	prep	before,mounted
R4896	T8024	T8025	auxpass	being,photographed
R4897	T8025	T8023	pcomp	photographed,before
R4898	T8026	T8017	punct	.,mounted
R4899	T8028	T8029	det	The,phenotype
R49	T257	T255	dobj	activities,implicating
R490	T1089	T1090	punct	-,7
R4900	T8029	T8032	nsubj	phenotype,is
R4901	T8030	T8031	npadvmod	huntingtin,deficient
R4902	T8031	T8029	amod	deficient,phenotype
R4903	T8033	T8034	advmod	fully,penetrant
R4904	T8034	T8032	acomp	penetrant,is
R4905	T8035	T8032	prep	at,is
R4906	T8036	T8035	pobj	each,at
R4907	T8037	T8036	prep	of,each
R4908	T8038	T8039	det	the,stages
R4909	T8039	T8037	pobj	stages,of
R491	T1090	T1088	prep	7,3
R4910	T8040	T8041	dep	that,assessed
R4911	T8041	T8039	relcl	assessed,stages
R4912	T8042	T8041	auxpass	were,assessed
R4913	T8043	T8044	punct	[,10
R4914	T8044	T8041	parataxis	10,assessed
R4915	T8045	T8044	punct	],10
R4916	T8046	T8032	punct	.,is
R4917	T8048	T8049	quantmod	Three,six
R4918	T8049	T8051	nummod	six,embryos
R4919	T8050	T8049	quantmod	to,six
R492	T1091	T1088	punct	],3
R4920	T8051	T8052	nsubjpass	embryos,evaluated
R4921	T8053	T8052	auxpass	were,evaluated
R4922	T8054	T8052	prep	for,evaluated
R4923	T8055	T8056	det	each,marker
R4924	T8056	T8054	pobj	marker,for
R4925	T8057	T8052	punct	", ",evaluated
R4926	T8058	T8052	prep	with,evaluated
R4927	T8059	T8060	det	every,embryo
R4928	T8060	T8061	nsubj	embryo,exhibiting
R4929	T8061	T8058	pcomp	exhibiting,with
R493	T1092	T1060	punct	.,postulated
R4930	T8062	T8063	det	the,phenotype
R4931	T8063	T8061	dobj	phenotype,exhibiting
R4932	T8064	T8063	amod	same,phenotype
R4933	T8065	T8063	compound	mutant,phenotype
R4934	T8066	T8061	prep	in,exhibiting
R4935	T8067	T8068	det	each,case
R4936	T8068	T8066	pobj	case,in
R4937	T8069	T8052	punct	.,evaluated
R4938	T8126	T8127	compound	Alkaline,phosphatase
R4939	T8127	T8128	compound	phosphatase,staining
R494	T1094	T1095	poss	Huntingtin,number
R4940	T8129	T8128	prep	of,staining
R4941	T8130	T8131	amod	Primordial,Cells
R4942	T8131	T8129	pobj	Cells,of
R4943	T8132	T8131	compound	Germ,Cells
R4944	T8133	T8131	punct	(,Cells
R4945	T8134	T8131	appos	PCGs,Cells
R4946	T8135	T8131	punct	),Cells
R4947	T8137	T8138	prep	After,fixed
R4948	T8139	T8137	pobj	dissections,After
R4949	T8140	T8138	punct	", ",fixed
R495	T1095	T1098	nsubj	number,suggest
R4950	T8141	T8138	nsubjpass	embryos,fixed
R4951	T8142	T8138	auxpass	were,fixed
R4952	T8143	T8138	prep	in,fixed
R4953	T8144	T8145	nummod	4,%
R4954	T8145	T8146	compound	%,paraformaldehyde
R4955	T8146	T8143	pobj	paraformaldehyde,in
R4956	T8147	T8138	advmod	briefly,fixed
R4957	T8148	T8138	cc	and,fixed
R4958	T8149	T8138	conj	washed,fixed
R4959	T8150	T8149	cc	and,washed
R496	T1096	T1094	case	's,Huntingtin
R4960	T8151	T8149	conj	stored,washed
R4961	T8152	T8151	prep	in,stored
R4962	T8153	T8154	nummod	1,PBS
R4963	T8154	T8152	pobj	PBS,in
R4964	T8155	T8153	punct	×,1
R4965	T8156	T8154	punct	/,PBS
R4966	T8157	T8158	compound	0.1,%
R4967	T8158	T8159	compound	%,TX
R4968	T8159	T8154	appos	TX,PBS
R4969	T8160	T8159	punct	-,TX
R497	T1097	T1095	amod	large,number
R4970	T8161	T8159	nummod	100,TX
R4971	T8162	T8151	prep	at,stored
R4972	T8163	T8164	nummod	4,°C
R4973	T8164	T8162	pobj	°C,at
R4974	T8165	T8138	punct	.,fixed
R4975	T8167	T8168	nsubjpass	Embryos,washed
R4976	T8169	T8168	auxpass	were,washed
R4977	T8170	T8168	advmod	once,washed
R4978	T8171	T8168	prep	with,washed
R4979	T8172	T8173	compound	Tris,Maleate
R498	T1099	T1095	prep	of,number
R4980	T8173	T8175	compound	Maleate,Buffer
R4981	T8174	T8173	punct	-,Maleate
R4982	T8175	T8171	pobj	Buffer,with
R4983	T8176	T8177	punct	(,MgCl2
R4984	T8177	T8175	parataxis	MgCl2,Buffer
R4985	T8178	T8179	nummod	25,mM
R4986	T8179	T8180	compound	mM,Maleate
R4987	T8180	T8177	dep	Maleate,MgCl2
R4988	T8181	T8180	compound	Tris,Maleate
R4989	T8182	T8180	punct	-,Maleate
R499	T1100	T1101	amod	diverse,partners
R4990	T8183	T8177	punct	", ",MgCl2
R4991	T8184	T8185	nsubj	pH,9.0
R4992	T8185	T8177	ccomp	9.0,MgCl2
R4993	T8186	T8185	punct	=,9.0
R4994	T8187	T8177	punct	", ",MgCl2
R4995	T8188	T8189	nummod	0.8,mM
R4996	T8189	T8177	compound	mM,MgCl2
R4997	T8190	T8177	punct	),MgCl2
R4998	T8191	T8168	cc	and,washed
R4999	T8192	T8193	auxpass	were,incubated
R5	T208	T209	det	the,gene
R50	T258	T255	prep	in,implicating
R500	T1101	T1099	pobj	partners,of
R5000	T8193	T8168	conj	incubated,washed
R5001	T8194	T8193	advmod	subsequently,incubated
R5002	T8195	T8193	prep	in,incubated
R5003	T8196	T8197	compound	alkaline,phosphatase
R5004	T8197	T8198	compound	phosphatase,solution
R5005	T8198	T8195	pobj	solution,in
R5006	T8199	T8198	compound	staining,solution
R5007	T8200	T8201	punct	(,mg
R5008	T8201	T8193	parataxis	mg,incubated
R5009	T8202	T8203	nummod	25,mM
R501	T1102	T1101	amod	cytoplasmic,partners
R5010	T8203	T8204	compound	mM,Maleate
R5011	T8204	T8201	dep	Maleate,mg
R5012	T8205	T8204	compound	Tris,Maleate
R5013	T8206	T8204	punct	-,Maleate
R5014	T8207	T8201	punct	", ",mg
R5015	T8208	T8209	nsubj	pH,9.0
R5016	T8209	T8201	ccomp	9.0,mg
R5017	T8210	T8209	punct	=,9.0
R5018	T8211	T8201	punct	", ",mg
R5019	T8212	T8213	nummod	0.8,mM
R502	T1103	T1102	cc	and,cytoplasmic
R5020	T8213	T8214	compound	mM,MgCl2
R5021	T8214	T8201	dep	MgCl2,mg
R5022	T8215	T8201	punct	", ",mg
R5023	T8216	T8217	nummod	0.4,mg
R5024	T8217	T8218	nmod	mg,phosphate
R5025	T8218	T8201	dep	phosphate,mg
R5026	T8219	T8220	punct	/,ml
R5027	T8220	T8217	prep	ml,mg
R5028	T8221	T8222	compound	alpha,naphthyl
R5029	T8222	T8218	compound	naphthyl,phosphate
R503	T1104	T1102	conj	nuclear,cytoplasmic
R5030	T8223	T8222	punct	-,naphthyl
R5031	T8224	T8201	punct	", ",mg
R5032	T8225	T8201	nummod	1,mg
R5033	T8226	T8227	punct	/,ml
R5034	T8227	T8201	prep	ml,mg
R5035	T8228	T8201	amod	Fast,mg
R5036	T8229	T8201	punct	-,mg
R5037	T8230	T8201	amod	Red,mg
R5038	T8231	T8201	punct	),mg
R5039	T8232	T8168	punct	.,washed
R504	T1105	T1101	compound	protein,partners
R5040	T8234	T8235	amod	Stained,embryos
R5041	T8235	T8236	nsubjpass	embryos,washed
R5042	T8237	T8236	auxpass	were,washed
R5043	T8238	T8236	prep	in,washed
R5044	T8239	T8240	nummod	1,PBS
R5045	T8240	T8238	pobj	PBS,in
R5046	T8241	T8239	punct	×,1
R5047	T8242	T8243	punct	/,TX
R5048	T8243	T8240	prep	TX,PBS
R5049	T8244	T8245	nummod	0.1,%
R505	T1106	T1101	compound	binding,partners
R5050	T8245	T8243	compound	%,TX
R5051	T8246	T8243	punct	-,TX
R5052	T8247	T8243	nummod	100,TX
R5053	T8248	T8236	punct	.,washed
R5054	T8266	T8267	amod	Whole,embryo
R5055	T8267	T8268	compound	embryo,culture
R5056	T8270	T8271	nsubjpass	Embryos,dissected
R5057	T8272	T8271	auxpass	were,dissected
R5058	T8273	T8271	prep	at,dissected
R5059	T8274	T8273	pobj	E7.5,at
R506	T1107	T1098	advmod	strongly,suggest
R5060	T8275	T8271	cc	and,dissected
R5061	T8276	T8271	conj	washed,dissected
R5062	T8277	T8276	prep	in,washed
R5063	T8278	T8277	pobj	DMEM,in
R5064	T8279	T8271	punct	.,dissected
R5065	T8281	T8282	nsubjpass	Embryos,cultured
R5066	T8283	T8282	auxpass	were,cultured
R5067	T8284	T8282	advmod	then,cultured
R5068	T8285	T8282	advmod	individually,cultured
R5069	T8286	T8282	prep	in,cultured
R507	T1108	T1109	mark	that,participate
R5070	T8287	T8288	nummod	1,ml
R5071	T8288	T8286	pobj	ml,in
R5072	T8289	T8288	prep	of,ml
R5073	T8290	T8291	compound	culture,media
R5074	T8291	T8289	pobj	media,of
R5075	T8292	T8291	punct	(,media
R5076	T8293	T8294	nummod	75,%
R5077	T8294	T8295	nmod	%,serum
R5078	T8295	T8291	appos	serum,media
R5079	T8296	T8297	advmod	immediately,centrifuged
R508	T1109	T1098	ccomp	participate,suggest
R5080	T8297	T8295	amod	centrifuged,serum
R5081	T8298	T8295	compound	rat,serum
R5082	T8299	T8295	cc	and,serum
R5083	T8300	T8301	nummod	25,%
R5084	T8301	T8302	compound	%,DMEM
R5085	T8302	T8295	conj	DMEM,serum
R5086	T8303	T8304	punct	[,56
R5087	T8304	T8291	parataxis	56,media
R5088	T8305	T8304	punct	],56
R5089	T8306	T8291	punct	),media
R509	T1110	T1109	nsubj	huntingtin,participate
R5090	T8307	T8282	prep	for,cultured
R5091	T8308	T8309	nummod	24,hours
R5092	T8309	T8307	pobj	hours,for
R5093	T8310	T8311	prep	while,rotating
R5094	T8311	T8282	advcl	rotating,cultured
R5095	T8312	T8311	prep	in,rotating
R5096	T8313	T8314	det	a,incubator
R5097	T8314	T8312	pobj	incubator,in
R5098	T8315	T8316	nummod	37,°C
R5099	T8316	T8314	compound	°C,incubator
R51	T259	T258	pobj	nutrition,in
R510	T1111	T1109	aux	may,participate
R5100	T8317	T8311	prep	in,rotating
R5101	T8318	T8319	nummod	5,%
R5102	T8319	T8320	compound	%,CO2
R5103	T8320	T8317	pobj	CO2,in
R5104	T8321	T8282	punct	.,cultured
R5105	T8323	T8324	nsubjpass	Embryos,fixed
R5106	T8325	T8324	auxpass	were,fixed
R5107	T8326	T8324	advmod	then,fixed
R5108	T8327	T8324	prep	in,fixed
R5109	T8328	T8329	nummod	4,%
R511	T1112	T1109	prep	in,participate
R5110	T8329	T8330	compound	%,paraformaldehyde
R5111	T8330	T8327	pobj	paraformaldehyde,in
R5112	T8331	T8324	prep	for,fixed
R5113	T8332	T8331	pobj	analysis,for
R5114	T8333	T8324	punct	.,fixed
R5115	T8416	T8417	compound	AVE,displacement
R5116	T8417	T8418	nsubj	displacement,occur
R5117	T8419	T8417	cc	and,displacement
R5118	T8420	T8421	amod	anterior,induction
R5119	T8421	T8417	conj	induction,displacement
R512	T1113	T1114	det	a,variety
R5120	T8422	T8421	compound	neurectoderm,induction
R5121	T8423	T8418	advmod	normally,occur
R5122	T8424	T8418	prep	in,occur
R5123	T8425	T8426	det	the,absence
R5124	T8426	T8424	pobj	absence,in
R5125	T8427	T8426	prep	of,absence
R5126	T8428	T8427	pobj	huntingtin,of
R5127	T8429	T8418	punct	.,occur
R5128	T8431	T8432	amod	Whole,mount
R5129	T8432	T8433	nmod	mount,analysis
R513	T1114	T1112	pobj	variety,in
R5130	T8433	T8437	nsubj	analysis,reveals
R5131	T8434	T8435	advmod	in,situ
R5132	T8435	T8433	amod	situ,analysis
R5133	T8436	T8433	compound	hybridization,analysis
R5134	T8438	T8433	prep	of,analysis
R5135	T8439	T8438	pobj	Otx2,of
R5136	T8440	T8441	punct	(,B
R5137	T8441	T8439	parataxis	B,Otx2
R5138	T8442	T8441	nmod	A,B
R5139	T8443	T8441	punct	",",B
R514	T1115	T1114	prep	of,variety
R5140	T8444	T8441	punct	),B
R5141	T8445	T8439	cc	and,Otx2
R5142	T8446	T8439	conj	Hesx,Otx2
R5143	T8447	T8448	punct	(,C
R5144	T8448	T8446	parataxis	C,Hesx
R5145	T8449	T8450	punct	-,F
R5146	T8450	T8448	prep	F,C
R5147	T8451	T8448	punct	),C
R5148	T8452	T8433	prep	in,analysis
R5149	T8453	T8454	nmod	E7.5,embryos
R515	T1116	T1117	amod	cellular,processes
R5150	T8454	T8452	pobj	embryos,in
R5151	T8455	T8454	amod	normal,embryos
R5152	T8456	T8457	punct	(,E
R5153	T8457	T8455	parataxis	E,normal
R5154	T8458	T8457	nmod	A,E
R5155	T8459	T8457	punct	",",E
R5156	T8460	T8457	nmod	C,E
R5157	T8461	T8457	punct	",",E
R5158	T8462	T8457	punct	),E
R5159	T8463	T8455	cc	and,normal
R516	T1117	T1115	pobj	processes,of
R5160	T8464	T8455	conj	mutant,normal
R5161	T8465	T8466	punct	(,F
R5162	T8466	T8464	parataxis	F,mutant
R5163	T8467	T8466	nmod	B,F
R5164	T8468	T8466	punct	",",F
R5165	T8469	T8466	nmod	D,F
R5166	T8470	T8466	punct	",",F
R5167	T8471	T8466	punct	),F
R5168	T8472	T8473	mark	that,develop
R5169	T8473	T8437	ccomp	develop,reveals
R517	T1118	T1119	dep	that,range
R5170	T8474	T8473	nsubj	neuroectoderm,develop
R5171	T8475	T8474	cc	and,neuroectoderm
R5172	T8476	T8477	amod	anterior,endoderm
R5173	T8477	T8474	conj	endoderm,neuroectoderm
R5174	T8478	T8477	amod	visceral,endoderm
R5175	T8479	T8477	punct	(,endoderm
R5176	T8480	T8477	appos	AVE,endoderm
R5177	T8481	T8473	punct	),develop
R5178	T8482	T8473	advmod	normally,develop
R5179	T8483	T8473	prep	in,develop
R518	T1119	T1117	relcl	range,processes
R5180	T8484	T8485	npadvmod	huntingtin,deficient
R5181	T8485	T8486	amod	deficient,embryos
R5182	T8486	T8483	pobj	embryos,in
R5183	T8487	T8473	punct	", ",develop
R5184	T8488	T8489	mark	although,reduced
R5185	T8489	T8473	advcl	reduced,develop
R5186	T8490	T8491	det	the,domain
R5187	T8491	T8489	nsubjpass	domain,reduced
R5188	T8492	T8493	compound	neuroectoderm,expression
R5189	T8493	T8491	compound	expression,domain
R519	T1120	T1119	prep	from,range
R5190	T8494	T8489	auxpass	is,reduced
R5191	T8495	T8437	punct	.,reveals
R5192	T8497	T8498	amod	Asymmetrical,expression
R5193	T8498	T8499	nsubj	expression,suggests
R5194	T8500	T8498	prep	of,expression
R5195	T8501	T8500	pobj	Hesx,of
R5196	T8502	T8498	prep	in,expression
R5197	T8503	T8504	compound	mutant,embryos
R5198	T8504	T8502	pobj	embryos,in
R5199	T8505	T8506	punct	(,F
R52	T260	T259	cc	or,nutrition
R520	T1121	T1120	pobj	trafficking,from
R5200	T8506	T8498	parataxis	F,expression
R5201	T8507	T8506	punct	),F
R5202	T8508	T8509	mark	that,maintained
R5203	T8509	T8499	ccomp	maintained,suggests
R5204	T8510	T8511	amod	left,right
R5205	T8511	T8513	amod	right,control
R5206	T8512	T8511	punct	-,right
R5207	T8513	T8509	nsubjpass	control,maintained
R5208	T8514	T8513	amod	transcriptional,control
R5209	T8515	T8509	auxpass	is,maintained
R521	T1122	T1121	prep	of,trafficking
R5210	T8516	T8499	punct	.,suggests
R5211	T8518	T8519	compound	Hnf3β,expression
R5212	T8519	T8520	nsubj	expression,extends
R5213	T8521	T8519	prep	in,expression
R5214	T8522	T8523	det	the,endoderm
R5215	T8523	T8521	pobj	endoderm,in
R5216	T8524	T8523	amod	definitive,endoderm
R5217	T8525	T8520	prep	around,extends
R5218	T8526	T8527	det	the,tip
R5219	T8527	T8525	pobj	tip,around
R522	T1123	T1124	compound	growth,complexes
R5220	T8528	T8527	amod	distal,tip
R5221	T8529	T8520	cc	and,extends
R5222	T8530	T8531	auxpass	is,reduced
R5223	T8531	T8520	conj	reduced,extends
R5224	T8532	T8531	prep	in,reduced
R5225	T8533	T8534	det	the,AVE
R5226	T8534	T8532	pobj	AVE,in
R5227	T8535	T8536	punct	(,*
R5228	T8536	T8534	punct	*,AVE
R5229	T8537	T8536	punct	),*
R523	T1124	T1122	pobj	complexes,of
R5230	T8538	T8531	prep	in,reduced
R5231	T8539	T8540	preconj	both,embryos
R5232	T8540	T8538	pobj	embryos,in
R5233	T8541	T8540	amod	normal,embryos
R5234	T8542	T8543	punct	(,I
R5235	T8543	T8541	parataxis	I,normal
R5236	T8544	T8543	nmod	G,I
R5237	T8545	T8543	punct	",",I
R5238	T8546	T8543	punct	),I
R5239	T8547	T8541	cc	and,normal
R524	T1125	T1124	compound	factor,complexes
R5240	T8548	T8541	conj	mutant,normal
R5241	T8549	T8550	punct	(,J
R5242	T8550	T8540	parataxis	J,embryos
R5243	T8551	T8550	nmod	H,J
R5244	T8552	T8550	punct	",",J
R5245	T8553	T8550	punct	),J
R5246	T8554	T8520	punct	.,extends
R5247	T8556	T8557	advcl	Taken,suggest
R5248	T8558	T8556	advmod	together,Taken
R5249	T8559	T8557	punct	", ",suggest
R525	T1126	T1120	prep	to,from
R5250	T8560	T8561	det	these,results
R5251	T8561	T8557	nsubj	results,suggest
R5252	T8562	T8563	amod	normal,ectoderm
R5253	T8563	T8557	dobj	ectoderm,suggest
R5254	T8564	T8563	cc	and,ectoderm
R5255	T8565	T8563	conj	endoderm,ectoderm
R5256	T8566	T8563	appos	induction,ectoderm
R5257	T8567	T8566	cc	and,induction
R5258	T8568	T8566	conj	localization,induction
R5259	T8569	T8557	prep	in,suggest
R526	T1127	T1128	compound	gene,transcription
R5260	T8570	T8571	nmod	Hdhex4,embryos
R5261	T8571	T8569	pobj	embryos,in
R5262	T8572	T8570	punct	/,Hdhex4
R5263	T8573	T8570	nummod	5,Hdhex4
R5264	T8574	T8570	punct	/,Hdhex4
R5265	T8575	T8570	appos	Hdhex4,Hdhex4
R5266	T8576	T8575	punct	/,Hdhex4
R5267	T8577	T8575	nummod	5,Hdhex4
R5268	T8578	T8557	punct	.,suggest
R5269	T8580	T8581	nsubjpass	Embryos,shown
R527	T1128	T1126	pobj	transcription,to
R5270	T8582	T8581	auxpass	are,shown
R5271	T8583	T8581	prep	in,shown
R5272	T8584	T8585	amod	lateral,views
R5273	T8585	T8583	pobj	views,in
R5274	T8586	T8581	punct	", ",shown
R5275	T8587	T8588	mark	with,to
R5276	T8588	T8581	advcl	to,shown
R5277	T8589	T8588	nsubj	anterior,to
R5278	T8590	T8591	det	the,left
R5279	T8591	T8588	pobj	left,to
R528	T1129	T1130	punct	(,reviewed
R5280	T8592	T8588	prep	in,to
R5281	T8593	T8594	det	all,pictures
R5282	T8594	T8592	pobj	pictures,in
R5283	T8595	T8594	prep	with,pictures
R5284	T8596	T8597	det	the,exception
R5285	T8597	T8595	pobj	exception,with
R5286	T8598	T8597	prep	of,exception
R5287	T8599	T8598	pobj	E,of
R5288	T8600	T8599	cc	and,E
R5289	T8601	T8599	conj	F,E
R529	T1130	T1109	parataxis	reviewed,participate
R5290	T8602	T8581	punct	.,shown
R5291	T8604	T8605	nsubjpass	Embryos,viewed
R5292	T8606	T8605	auxpass	are,viewed
R5293	T8607	T8605	prep	from,viewed
R5294	T8608	T8609	det	the,aspect
R5295	T8609	T8607	pobj	aspect,from
R5296	T8610	T8609	amod	anterior,aspect
R5297	T8611	T8605	prep	in,viewed
R5298	T8612	T8611	pobj	E,in
R5299	T8613	T8612	cc	and,E
R53	T261	T259	conj	patterning,nutrition
R530	T1131	T1130	prep	in,reviewed
R5300	T8614	T8612	conj	F,E
R5301	T8615	T8605	punct	.,viewed
R5306	T8760	T8761	nsubjpass	Huntingtin,required
R5307	T8762	T8761	auxpass	is,required
R5308	T8763	T8761	prep	for,required
R5309	T8764	T8763	pobj	formation,for
R531	T1132	T1131	punct	[,in
R5310	T8765	T8764	prep	of,formation
R5311	T8766	T8767	amod	anterior,streak
R5312	T8767	T8765	pobj	streak,of
R5313	T8768	T8767	amod	primitive,streak
R5314	T8769	T8767	cc	and,streak
R5315	T8770	T8771	amod	paraxial,mesoderm
R5316	T8771	T8767	conj	mesoderm,streak
R5317	T8772	T8761	punct	.,required
R5318	T8774	T8775	amod	Whole,mount
R5319	T8775	T8776	nmod	mount,hybridizations
R532	T1133	T1134	nummod	5,9
R5320	T8776	T8780	nsubj	hybridizations,shows
R5321	T8777	T8775	cc	and,mount
R5322	T8778	T8775	conj	section,mount
R5323	T8779	T8776	nmod	insitu,hybridizations
R5324	T8781	T8776	prep	of,hybridizations
R5325	T8782	T8783	compound	E7.5,embryos
R5326	T8783	T8781	pobj	embryos,of
R5327	T8784	T8785	nsubjpass	T,expressed
R5328	T8785	T8780	advcl	expressed,shows
R5329	T8786	T8784	punct	(,T
R533	T1134	T1131	pobj	9,in
R5330	T8787	T8784	appos	Brachyury,T
R5331	T8788	T8784	punct	),T
R5332	T8789	T8790	punct	(,A
R5333	T8790	T8784	parataxis	A,T
R5334	T8791	T8792	punct	-,D
R5335	T8792	T8790	prep	D,A
R5336	T8793	T8790	punct	),A
R5337	T8794	T8785	auxpass	is,expressed
R5338	T8795	T8785	prep	in,expressed
R5339	T8796	T8797	det	the,streak
R534	T1135	T1134	punct	",",9
R5340	T8797	T8795	pobj	streak,in
R5341	T8798	T8797	amod	primitive,streak
R5342	T8799	T8797	punct	", ",streak
R5343	T8800	T8797	appos	node,streak
R5344	T8801	T8797	punct	", ",streak
R5345	T8802	T8803	amod	axial,mesoderm
R5346	T8803	T8797	appos	mesoderm,streak
R5347	T8804	T8785	cc	and,expressed
R5348	T8805	T8806	nsubjpass	Evx1,expressed
R5349	T8806	T8785	conj	expressed,expressed
R535	T1136	T1134	nummod	8,9
R5350	T8807	T8808	punct	(,E
R5351	T8808	T8805	parataxis	E,Evx1
R5352	T8809	T8810	punct	-,F
R5353	T8810	T8808	prep	F,E
R5354	T8811	T8808	punct	),E
R5355	T8812	T8806	auxpass	is,expressed
R5356	T8813	T8806	prep	in,expressed
R5357	T8814	T8815	det	the,streak
R5358	T8815	T8813	pobj	streak,in
R5359	T8816	T8815	amod	primitive,streak
R536	T1137	T1134	punct	",",9
R5360	T8817	T8806	punct	", ",expressed
R5361	T8818	T8819	advmod	most,strongly
R5362	T8819	T8806	advmod	strongly,expressed
R5363	T8820	T8819	prep	in,strongly
R5364	T8821	T8822	det	the,embryos
R5365	T8822	T8820	pobj	embryos,in
R5366	T8823	T8824	amod	proximal,streak
R5367	T8824	T8822	nmod	streak,embryos
R5368	T8825	T8826	amod	wild,type
R5369	T8826	T8822	compound	type,embryos
R537	T1138	T1130	punct	],reviewed
R5370	T8827	T8826	punct	-,type
R5371	T8828	T8780	punct	.,shows
R5372	T8830	T8831	advmod	However,expressed
R5373	T8832	T8831	punct	", ",expressed
R5374	T8833	T8831	prep	in,expressed
R5375	T8834	T8835	compound	mutant,embryos
R5376	T8835	T8833	pobj	embryos,in
R5377	T8836	T8831	punct	", ",expressed
R5378	T8837	T8838	preconj	both,T
R5379	T8838	T8831	nsubjpass	T,expressed
R538	T1139	T1098	punct	.,suggest
R5380	T8839	T8840	punct	(,D
R5381	T8840	T8838	parataxis	D,T
R5382	T8841	T8840	nmod	B,D
R5383	T8842	T8840	punct	", ",D
R5384	T8843	T8840	punct	),D
R5385	T8844	T8838	cc	and,T
R5386	T8845	T8838	conj	Evx1,T
R5387	T8846	T8847	punct	(,F
R5388	T8847	T8845	parataxis	F,Evx1
R5389	T8848	T8847	punct	),F
R539	T1141	T1142	advmod	However,defined
R5390	T8849	T8831	auxpass	are,expressed
R5391	T8850	T8831	advmod	ectopically,expressed
R5392	T8851	T8831	prep	in,expressed
R5393	T8852	T8853	det	the,region
R5394	T8853	T8851	pobj	region,in
R5395	T8854	T8853	amod	extraembryonic,region
R5396	T8855	T8831	punct	.,expressed
R5397	T8857	T8858	compound	Wnt3A,expression
R5398	T8858	T8859	nsubjpass	expression,reduced
R5399	T8860	T8859	auxpass	is,reduced
R54	T262	T259	prep	of,nutrition
R540	T1143	T1142	punct	", ",defined
R5400	T8861	T8859	prep	in,reduced
R5401	T8862	T8863	compound	mutant,embryos
R5402	T8863	T8861	pobj	embryos,in
R5403	T8864	T8865	punct	(,H
R5404	T8865	T8863	parataxis	H,embryos
R5405	T8866	T8865	punct	),H
R5406	T8867	T8859	punct	", ",reduced
R5407	T8868	T8869	mark	although,is
R5408	T8869	T8859	advcl	is,reduced
R5409	T8870	T8871	det	the,localization
R541	T1144	T1142	prep	despite,defined
R5410	T8871	T8869	nsubj	localization,is
R5411	T8872	T8871	prep	of,localization
R5412	T8873	T8874	poss	its,expression
R5413	T8874	T8872	pobj	expression,of
R5414	T8875	T8871	prep	to,localization
R5415	T8876	T8877	det	the,streak
R5416	T8877	T8875	pobj	streak,to
R5417	T8878	T8877	amod	proximal,streak
R5418	T8879	T8880	det	the,same
R5419	T8880	T8869	attr	same,is
R542	T1145	T1146	det	the,importance
R5420	T8881	T8880	prep	as,same
R5421	T8882	T8881	prep	in,as
R5422	T8883	T8884	amod	wild,type
R5423	T8884	T8886	compound	type,embryos
R5424	T8885	T8884	punct	-,type
R5425	T8886	T8882	pobj	embryos,in
R5426	T8887	T8888	punct	(,G
R5427	T8888	T8869	parataxis	G,is
R5428	T8889	T8888	punct	),G
R5429	T8890	T8859	punct	.,reduced
R543	T1146	T1144	pobj	importance,despite
R5430	T8892	T8893	nsubj	Analysis,reveals
R5431	T8894	T8892	prep	of,Analysis
R5432	T8895	T8896	amod	paraxial,Tbx6
R5433	T8896	T8894	pobj	Tbx6,of
R5434	T8897	T8896	compound	mesoderm,Tbx6
R5435	T8898	T8896	compound	markers,Tbx6
R5436	T8899	T8900	punct	(,J
R5437	T8900	T8896	parataxis	J,Tbx6
R5438	T8901	T8900	nmod	I,J
R5439	T8902	T8900	punct	",",J
R544	T1147	T1146	amod	potential,importance
R5440	T8903	T8900	punct	),J
R5441	T8904	T8896	cc	and,Tbx6
R5442	T8905	T8896	conj	Dll1,Tbx6
R5443	T8906	T8907	punct	(,L
R5444	T8907	T8905	parataxis	L,Dll1
R5445	T8908	T8907	nmod	K,L
R5446	T8909	T8907	punct	",",L
R5447	T8910	T8907	punct	),L
R5448	T8911	T8893	punct	", ",reveals
R5449	T8912	T8913	mark	that,reduced
R545	T1148	T1146	prep	of,importance
R5450	T8913	T8893	ccomp	reduced,reveals
R5451	T8914	T8915	det	these,markers
R5452	T8915	T8913	nsubjpass	markers,reduced
R5453	T8916	T8913	auxpass	are,reduced
R5454	T8917	T8913	prep	in,reduced
R5455	T8918	T8919	compound	mutant,embryos
R5456	T8919	T8917	pobj	embryos,in
R5457	T8920	T8921	punct	(,L
R5458	T8921	T8913	parataxis	L,reduced
R5459	T8922	T8921	nmod	J,L
R546	T1149	T1150	poss	huntingtin,function
R5460	T8923	T8921	punct	",",L
R5461	T8924	T8921	punct	),L
R5462	T8925	T8913	punct	", ",reduced
R5463	T8926	T8913	advcl	suggesting,reduced
R5464	T8927	T8928	amod	impaired,production
R5465	T8928	T8926	dobj	production,suggesting
R5466	T8929	T8930	amod	paraxial,mesoderm
R5467	T8930	T8928	compound	mesoderm,production
R5468	T8931	T8928	prep	in,production
R5469	T8932	T8933	det	the,absence
R547	T1150	T1148	pobj	function,of
R5470	T8933	T8931	pobj	absence,in
R5471	T8934	T8933	prep	of,absence
R5472	T8935	T8934	pobj	huntingtin,of
R5473	T8936	T8893	punct	.,reveals
R5474	T8938	T8939	nsubjpass	Embryos,shown
R5475	T8940	T8938	prep	in,Embryos
R5476	T8941	T8940	pobj	A,in
R5477	T8942	T8943	punct	-,H
R5478	T8943	T8941	prep	H,A
R5479	T8944	T8939	auxpass	are,shown
R548	T1151	T1149	case	's,huntingtin
R5480	T8945	T8939	prep	in,shown
R5481	T8946	T8947	det	a,view
R5482	T8947	T8945	pobj	view,in
R5483	T8948	T8947	amod	lateral,view
R5484	T8949	T8950	mark	with,oriented
R5485	T8950	T8939	advcl	oriented,shown
R5486	T8951	T8950	nsubj	anterior,oriented
R5487	T8952	T8950	prep	to,oriented
R5488	T8953	T8954	det	the,left
R5489	T8954	T8952	pobj	left,to
R549	T1152	T1150	amod	normal,function
R5490	T8955	T8939	punct	.,shown
R5491	T8957	T8958	nsubjpass	Embryos,shown
R5492	T8959	T8957	prep	in,Embryos
R5493	T8960	T8959	pobj	I,in
R5494	T8961	T8962	punct	-,L
R5495	T8962	T8960	prep	L,I
R5496	T8963	T8958	auxpass	are,shown
R5497	T8964	T8958	prep	in,shown
R5498	T8965	T8966	det	a,view
R5499	T8966	T8964	pobj	view,in
R55	T263	T264	det	the,embryo
R550	T1153	T1146	prep	to,importance
R5500	T8967	T8966	amod	posterior,view
R5501	T8968	T8969	punct	(,K
R5502	T8969	T8966	parataxis	K,view
R5503	T8970	T8969	nmod	I,K
R5504	T8971	T8969	punct	",",K
R5505	T8972	T8969	punct	),K
R5506	T8973	T8966	cc	or,view
R5507	T8974	T8975	advmod	near,posterior
R5508	T8975	T8976	amod	posterior,view
R5509	T8976	T8966	conj	view,view
R551	T1154	T1155	poss	our,understanding
R5510	T8977	T8978	punct	(,L
R5511	T8978	T8976	parataxis	L,view
R5512	T8979	T8978	nmod	J,L
R5513	T8980	T8978	punct	",",L
R5514	T8981	T8978	punct	),L
R5515	T8982	T8983	mark	with,oriented
R5516	T8983	T8958	advcl	oriented,shown
R5517	T8984	T8983	nsubj	proximal,oriented
R5518	T8985	T8983	prep	toward,oriented
R5519	T8986	T8987	det	the,top
R552	T1155	T1153	pobj	understanding,to
R5520	T8987	T8985	pobj	top,toward
R5521	T8988	T8958	punct	.,shown
R5522	T8990	T8991	prep	In,streak
R5523	T8992	T8993	punct	(,D
R5524	T8993	T8990	pobj	D,In
R5525	T8994	T8993	nmod	C,D
R5526	T8995	T8993	punct	",",D
R5527	T8996	T8991	punct	),streak
R5528	T8997	T8991	punct	", ",streak
R5529	T8998	T8999	nsubj	al,allantois
R553	T1156	T1155	prep	of,understanding
R5530	T8999	T8991	ccomp	allantois,streak
R5531	T9000	T8999	punct	=,allantois
R5532	T9001	T8991	punct	", ",streak
R5533	T9002	T9003	nsubj	a,amnion
R5534	T9003	T8991	ccomp	amnion,streak
R5535	T9004	T9003	punct	=,amnion
R5536	T9005	T8991	punct	", ",streak
R5537	T9006	T9007	nsubj	ch,chorion
R5538	T9007	T8991	ccomp	chorion,streak
R5539	T9008	T9007	punct	=,chorion
R554	T1157	T1158	advmod	how,causes
R5540	T9009	T8991	punct	", ",streak
R5541	T9010	T9011	nsubj	ee,node
R5542	T9011	T8991	ccomp	node,streak
R5543	T9012	T9011	punct	=,node
R5544	T9013	T9011	amod	embryonic,node
R5545	T9014	T9015	punct	(,N
R5546	T9015	T9011	parataxis	N,node
R5547	T9016	T9015	punct	),N
R5548	T9017	T8991	punct	", ",streak
R5549	T9018	T9019	nsubj	em,mesoderm
R555	T1158	T1156	pcomp	causes,of
R5550	T9019	T8991	ccomp	mesoderm,streak
R5551	T9020	T9019	punct	=,mesoderm
R5552	T9021	T9019	amod	extraembryonic,mesoderm
R5553	T9022	T8991	punct	", ",streak
R5554	T9023	T9024	nsubj	n,node
R5555	T9024	T8991	ccomp	node,streak
R5556	T9025	T9024	punct	=,node
R5557	T9026	T8991	punct	", ",streak
R5558	T9027	T8991	nsubj	ps,streak
R5559	T9028	T8991	punct	=,streak
R556	T1159	T1160	det	the,mutation
R5560	T9029	T8991	amod	primitive,streak
R5561	T9030	T8991	punct	.,streak
R5562	T9032	T9033	advmod	Rather,than
R5563	T9033	T9034	prep	than,exhibit
R5564	T9035	T9036	det	a,node
R5565	T9036	T9033	pobj	node,than
R5566	T9037	T9034	punct	", ",exhibit
R5567	T9038	T9039	compound	mutant,embryos
R5568	T9039	T9034	nsubj	embryos,exhibit
R5569	T9040	T9041	det	a,region
R557	T1160	T1158	nsubj	mutation,causes
R5570	T9041	T9034	dobj	region,exhibit
R5571	T9042	T9041	prep	of,region
R5572	T9043	T9044	amod	disorganized,cells
R5573	T9044	T9042	pobj	cells,of
R5574	T9045	T9046	punct	(,*
R5575	T9046	T9041	punct	*,region
R5576	T9047	T9046	punct	),*
R5577	T9048	T9034	prep	at,exhibit
R5578	T9049	T9050	det	the,extent
R5579	T9050	T9048	pobj	extent,at
R558	T1161	T1160	amod	dominant,mutation
R5580	T9051	T9050	amod	distal,extent
R5581	T9052	T9050	prep	of,extent
R5582	T9053	T9054	det	the,streak
R5583	T9054	T9052	pobj	streak,of
R5584	T9055	T9054	amod	short,streak
R5585	T9056	T9054	amod	primitive,streak
R5586	T9057	T9034	punct	.,exhibit
R5587	T9177	T9178	amod	Impaired,restriction
R5588	T9179	T9178	amod	regional,restriction
R5589	T9180	T9178	prep	of,restriction
R559	T1162	T1160	compound	polyglutamine,mutation
R5590	T9181	T9182	compound	gene,expression
R5591	T9182	T9180	pobj	expression,of
R5592	T9183	T9178	prep	in,restriction
R5593	T9184	T9185	npadvmod	huntingtin,deficient
R5594	T9185	T9186	amod	deficient,embryos
R5595	T9186	T9183	pobj	embryos,in
R5596	T9187	T9178	punct	.,restriction
R5597	T9189	T9190	compound	X,gal
R5598	T9190	T9192	compound	gal,staining
R5599	T9191	T9190	punct	-,gal
R56	T264	T262	pobj	embryo,of
R560	T1163	T1164	compound	HD,pathology
R5600	T9192	T9193	nsubj	staining,shows
R5601	T9194	T9192	prep	of,staining
R5602	T9195	T9196	compound	Nodal,LacZ
R5603	T9196	T9198	compound	LacZ,embryos
R5604	T9197	T9196	punct	-,LacZ
R5605	T9198	T9194	pobj	embryos,of
R5606	T9199	T9193	dobj	staining,shows
R5607	T9200	T9199	prep	in,staining
R5608	T9201	T9200	pobj	endoderm,in
R5609	T9202	T9199	prep	near,staining
R561	T1164	T1158	dobj	pathology,causes
R5610	T9203	T9204	det	the,node
R5611	T9204	T9202	pobj	node,near
R5612	T9205	T9204	prep	of,node
R5613	T9206	T9207	amod	normal,embryos
R5614	T9207	T9205	pobj	embryos,of
R5615	T9208	T9209	punct	(,C
R5616	T9209	T9199	parataxis	C,staining
R5617	T9210	T9209	nmod	A,C
R5618	T9211	T9209	punct	",",C
R5619	T9212	T9209	punct	),C
R562	T1165	T1142	punct	", ",defined
R5620	T9213	T9199	cc	but,staining
R5621	T9214	T9215	amod	broad,staining
R5622	T9215	T9199	conj	staining,staining
R5623	T9216	T9215	prep	in,staining
R5624	T9217	T9218	compound	mutant,embryos
R5625	T9218	T9216	pobj	embryos,in
R5626	T9219	T9220	punct	(,D
R5627	T9220	T9215	parataxis	D,staining
R5628	T9221	T9220	nmod	B,D
R5629	T9222	T9220	punct	", ",D
R563	T1166	T1167	poss	huntingtin,activities
R5630	T9223	T9220	punct	),D
R5631	T9224	T9193	punct	", ",shows
R5632	T9225	T9226	mark	although,is
R5633	T9226	T9193	advcl	is,shows
R5634	T9227	T9226	nsubj	expression,is
R5635	T9228	T9226	acomp	higher,is
R5636	T9229	T9226	prep	in,is
R5637	T9230	T9231	det	the,posterior
R5638	T9231	T9229	pobj	posterior,in
R5639	T9232	T9193	punct	.,shows
R564	T1167	T1142	nsubjpass	activities,defined
R5640	T9234	T9235	det	The,expression
R5641	T9235	T9238	nsubjpass	expression,lost
R5642	T9236	T9235	amod	tight,expression
R5643	T9237	T9235	compound	node,expression
R5644	T9239	T9235	prep	of,expression
R5645	T9240	T9239	pobj	Nodal,of
R5646	T9241	T9235	prep	in,expression
R5647	T9242	T9243	amod	normal,embryos
R5648	T9243	T9241	pobj	embryos,in
R5649	T9244	T9245	punct	(,C
R565	T1168	T1166	case	's,huntingtin
R5650	T9245	T9235	parataxis	C,expression
R5651	T9246	T9245	punct	),C
R5652	T9247	T9238	auxpass	is,lost
R5653	T9248	T9238	prep	in,lost
R5654	T9249	T9250	compound	mutant,embryos
R5655	T9250	T9248	pobj	embryos,in
R5656	T9251	T9252	punct	(,D
R5657	T9252	T9238	parataxis	D,lost
R5658	T9253	T9252	punct	),D
R5659	T9254	T9238	punct	", ",lost
R566	T1169	T1167	amod	precise,activities
R5660	T9255	T9238	advcl	consistent,lost
R5661	T9256	T9255	prep	with,consistent
R5662	T9257	T9258	det	the,loss
R5663	T9258	T9256	pobj	loss,with
R5664	T9259	T9258	prep	of,loss
R5665	T9260	T9261	det	a,node
R5666	T9261	T9259	pobj	node,of
R5667	T9262	T9261	amod	morphological,node
R5668	T9263	T9258	prep	in,loss
R5669	T9264	T9265	det	the,absence
R567	T1170	T1167	amod	molecular,activities
R5670	T9265	T9263	pobj	absence,in
R5671	T9266	T9265	prep	of,absence
R5672	T9267	T9266	pobj	huntingtin,of
R5673	T9268	T9238	punct	.,lost
R5674	T9270	T9271	amod	Whole,mount
R5675	T9271	T9272	nmod	mount,hybridization
R5676	T9272	T9276	nsubj	hybridization,reveals
R5677	T9273	T9271	cc	and,mount
R5678	T9274	T9275	advmod	in,situ
R5679	T9275	T9271	conj	situ,mount
R568	T1171	T1170	cc	and,molecular
R5680	T9277	T9272	prep	of,hybridization
R5681	T9278	T9279	compound	E7.5,day
R5682	T9279	T9280	compound	day,embryos
R5683	T9280	T9277	pobj	embryos,of
R5684	T9281	T9282	mark	that,detected
R5685	T9282	T9276	ccomp	detected,reveals
R5686	T9283	T9282	nsubjpass	Fgf8,detected
R5687	T9284	T9282	auxpass	is,detected
R5688	T9285	T9282	prep	in,detected
R5689	T9286	T9287	det	the,streak
R569	T1172	T1170	conj	cellular,molecular
R5690	T9287	T9285	pobj	streak,in
R5691	T9288	T9287	amod	proximal,streak
R5692	T9289	T9282	cc	and,detected
R5693	T9290	T9291	auxpass	is,downregulated
R5694	T9291	T9282	conj	downregulated,detected
R5695	T9292	T9291	prep	in,downregulated
R5696	T9293	T9292	pobj	cells,in
R5697	T9294	T9293	acl	migrating,cells
R5698	T9295	T9294	prep	out,migrating
R5699	T9296	T9295	prep	of,out
R57	T265	T264	amod	developing,embryo
R570	T1173	T1142	aux	have,defined
R5700	T9297	T9298	det	the,streak
R5701	T9298	T9296	pobj	streak,of
R5702	T9299	T9294	prep	in,migrating
R5703	T9300	T9301	amod	normal,embryos
R5704	T9301	T9299	pobj	embryos,in
R5705	T9302	T9303	punct	(,G
R5706	T9303	T9291	parataxis	G,downregulated
R5707	T9304	T9303	nmod	E,G
R5708	T9305	T9303	punct	",",G
R5709	T9306	T9303	punct	),G
R571	T1174	T1142	neg	not,defined
R5710	T9307	T9276	punct	.,reveals
R5711	T9309	T9310	prep	In,remains
R5712	T9311	T9309	pobj	contrast,In
R5713	T9312	T9310	punct	", ",remains
R5714	T9313	T9310	nsubj	Fgf8,remains
R5715	T9314	T9315	advmod	highly,expressed
R5716	T9315	T9310	oprd	expressed,remains
R5717	T9316	T9310	prep	in,remains
R5718	T9317	T9318	compound	mutant,embryos
R5719	T9318	T9316	pobj	embryos,in
R572	T1175	T1142	auxpass	been,defined
R5720	T9319	T9320	punct	(,H
R5721	T9320	T9310	parataxis	H,remains
R5722	T9321	T9320	nmod	F,H
R5723	T9322	T9320	punct	",",H
R5724	T9323	T9320	punct	),H
R5725	T9324	T9310	punct	.,remains
R5726	T9326	T9327	amod	Transient,expression
R5727	T9327	T9328	nsubjpass	expression,distinguished
R5728	T9329	T9327	prep	of,expression
R5729	T9330	T9329	pobj	Gsc,of
R573	T1176	T1142	punct	.,defined
R5730	T9331	T9327	prep	in,expression
R5731	T9332	T9333	det	the,endoderm
R5732	T9333	T9331	pobj	endoderm,in
R5733	T9334	T9333	amod	definitive,endoderm
R5734	T9335	T9333	acl	overlying,endoderm
R5735	T9336	T9337	det	the,region
R5736	T9337	T9335	dobj	region,overlying
R5737	T9338	T9337	amod	prospective,region
R5738	T9339	T9337	compound	head,region
R5739	T9340	T9335	prep	in,overlying
R574	T1178	T1179	advmod	Therefore,set
R5740	T9341	T9342	amod	normal,embryos
R5741	T9342	T9340	pobj	embryos,in
R5742	T9343	T9344	punct	(,K
R5743	T9344	T9335	parataxis	K,overlying
R5744	T9345	T9344	nmod	I,K
R5745	T9346	T9344	punct	",",K
R5746	T9347	T9344	punct	),K
R5747	T9348	T9328	auxpass	is,distinguished
R5748	T9349	T9328	prep	in,distinguished
R5749	T9350	T9351	amod	other,layers
R575	T1180	T1179	punct	", ",set
R5750	T9351	T9349	pobj	layers,in
R5751	T9352	T9351	compound	cell,layers
R5752	T9353	T9351	prep	in,layers
R5753	T9354	T9355	amod	normal,embryos
R5754	T9355	T9353	pobj	embryos,in
R5755	T9356	T9328	cc	but,distinguished
R5756	T9357	T9328	conj	remains,distinguished
R5757	T9358	T9357	oprd	unrestricted,remains
R5758	T9359	T9357	prep	in,remains
R5759	T9360	T9361	compound	mutant,embryos
R576	T1181	T1179	nsubj	we,set
R5760	T9361	T9359	pobj	embryos,in
R5761	T9362	T9363	punct	(,L
R5762	T9363	T9357	parataxis	L,remains
R5763	T9364	T9363	nmod	J,L
R5764	T9365	T9363	punct	",",L
R5765	T9366	T9363	punct	),L
R5766	T9367	T9328	punct	.,distinguished
R5767	T9369	T9370	advmod	Earlier,expression
R5768	T9370	T9372	nsubjpass	expression,maintained
R5769	T9371	T9370	amod	posterior,expression
R577	T1182	T1181	punct	", ",we
R5770	T9373	T9370	prep	of,expression
R5771	T9374	T9373	pobj	Gsc,of
R5772	T9375	T9372	auxpass	is,maintained
R5773	T9376	T9372	advmod	also,maintained
R5774	T9377	T9372	prep	in,maintained
R5775	T9378	T9379	compound	mutant,embryos
R5776	T9379	T9377	pobj	embryos,in
R5777	T9380	T9381	punct	(,J
R5778	T9381	T9379	parataxis	J,embryos
R5779	T9382	T9381	punct	),J
R578	T1183	T1181	cc	and,we
R5780	T9383	T9384	mark	while,regulated
R5781	T9384	T9372	advcl	regulated,maintained
R5782	T9385	T9384	nsubjpass	it,regulated
R5783	T9386	T9384	auxpass	is,regulated
R5784	T9387	T9384	advmod	down,regulated
R5785	T9388	T9384	punct	-,regulated
R5786	T9389	T9384	prep	in,regulated
R5787	T9390	T9391	amod	normal,embryos
R5788	T9391	T9389	pobj	embryos,in
R5789	T9392	T9393	punct	(,I
R579	T1184	T1181	conj	others,we
R5790	T9393	T9384	parataxis	I,regulated
R5791	T9394	T9393	punct	),I
R5792	T9395	T9372	punct	.,maintained
R5793	T9397	T9398	nsubjpass	Embryos,shown
R5794	T9399	T9400	punct	(,L
R5795	T9400	T9397	parataxis	L,Embryos
R5796	T9401	T9400	nmod	A,L
R5797	T9402	T9400	punct	",",L
R5798	T9403	T9400	nmod	B,L
R5799	T9404	T9400	punct	",",L
R58	T266	T231	punct	.,required
R580	T1185	T1179	punct	", ",set
R5800	T9405	T9400	nmod	E,L
R5801	T9406	T9400	punct	",",L
R5802	T9407	T9400	nmod	F,L
R5803	T9408	T9400	punct	",",L
R5804	T9409	T9400	nmod	G,L
R5805	T9410	T9400	punct	",",L
R5806	T9411	T9400	nmod	H,L
R5807	T9412	T9400	punct	",",L
R5808	T9413	T9400	nmod	I,L
R5809	T9414	T9400	punct	",",L
R581	T1186	T1179	prt	out,set
R5810	T9415	T9400	nmod	K,L
R5811	T9416	T9400	punct	",",L
R5812	T9417	T9400	punct	),L
R5813	T9418	T9398	auxpass	are,shown
R5814	T9419	T9398	prep	in,shown
R5815	T9420	T9421	det	a,view
R5816	T9421	T9419	pobj	view,in
R5817	T9422	T9421	amod	lateral,view
R5818	T9423	T9424	mark	with,oriented
R5819	T9424	T9398	advcl	oriented,shown
R582	T1187	T1188	aux	to,discover
R5820	T9425	T9424	nsubj	anterior,oriented
R5821	T9426	T9424	prep	to,oriented
R5822	T9427	T9428	det	the,left
R5823	T9428	T9426	pobj	left,to
R5824	T9429	T9398	punct	.,shown
R5825	T9431	T9432	nsubj	Embryos,are
R5826	T9433	T9434	punct	(,D
R5827	T9434	T9431	parataxis	D,Embryos
R5828	T9435	T9434	nmod	C,D
R5829	T9436	T9434	punct	",",D
R583	T1188	T1179	xcomp	discover,set
R5830	T9437	T9434	punct	),D
R5831	T9438	T9432	prep	in,are
R5832	T9439	T9440	det	a,view
R5833	T9440	T9438	pobj	view,in
R5834	T9441	T9440	amod	posterior,view
R5835	T9442	T9432	punct	.,are
R5836	T9641	T9642	amod	Normal,expression
R5837	T9643	T9642	prep	of,expression
R5838	T9644	T9645	amod	extraembryonic,markers
R5839	T9645	T9643	pobj	markers,of
R584	T1189	T1190	poss	huntingtin,activities
R5840	T9646	T9642	prep	in,expression
R5841	T9647	T9648	npadvmod	huntingtin,deficient
R5842	T9648	T9649	amod	deficient,embryos
R5843	T9649	T9646	pobj	embryos,in
R5844	T9650	T9642	punct	.,expression
R5845	T9652	T9653	amod	Whole,mount
R5846	T9653	T9654	nmod	mount,analysis
R5847	T9654	T9658	nsubj	analysis,reveals
R5848	T9655	T9656	advmod	in,situ
R5849	T9656	T9654	amod	situ,analysis
R585	T1190	T1188	dobj	activities,discover
R5850	T9657	T9654	compound	hybridization,analysis
R5851	T9659	T9654	prep	at,analysis
R5852	T9660	T9659	pobj	E7.5,at
R5853	T9661	T9654	prep	of,analysis
R5854	T9662	T9661	pobj	markers,of
R5855	T9663	T9662	prep	of,markers
R5856	T9664	T9665	det	the,tissues
R5857	T9665	T9663	pobj	tissues,of
R5858	T9666	T9665	amod	extraembryonic,tissues
R5859	T9667	T9668	advmod	grossly,normal
R586	T1191	T1189	case	's,huntingtin
R5860	T9668	T9669	amod	normal,expression
R5861	T9669	T9658	dobj	expression,reveals
R5862	T9670	T9658	prep	in,reveals
R5863	T9671	T9672	det	the,absence
R5864	T9672	T9670	pobj	absence,in
R5865	T9673	T9672	prep	of,absence
R5866	T9674	T9673	pobj	huntingtin,of
R5867	T9675	T9658	punct	.,reveals
R5868	T9677	T9678	nsubj	Hnf4,is
R5869	T9679	T9677	punct	", ",Hnf4
R587	T1192	T1190	amod	essential,activities
R5870	T9680	T9677	acl	expressed,Hnf4
R5871	T9681	T9680	prep	in,expressed
R5872	T9682	T9683	det	the,endoderm
R5873	T9683	T9681	pobj	endoderm,in
R5874	T9684	T9683	amod	visceral,endoderm
R5875	T9685	T9680	prep	at,expressed
R5876	T9686	T9687	det	the,junction
R5877	T9687	T9685	pobj	junction,at
R5878	T9688	T9687	prep	of,junction
R5879	T9689	T9690	amod	embryonic,ectoderm
R588	T1193	T1188	prep	by,discover
R5880	T9690	T9692	compound	ectoderm,junction
R5881	T9691	T9690	punct	-,ectoderm
R5882	T9692	T9688	pobj	junction,of
R5883	T9693	T9694	punct	(,A
R5884	T9694	T9680	parataxis	A,expressed
R5885	T9695	T9694	punct	),A
R5886	T9696	T9678	punct	", ",is
R5887	T9697	T9678	acomp	normal,is
R5888	T9698	T9678	prep	in,is
R5889	T9699	T9700	compound	mutant,embryos
R589	T1194	T1193	pcomp	studying,by
R5890	T9700	T9698	pobj	embryos,in
R5891	T9701	T9678	punct	", ",is
R5892	T9702	T9703	mark	although,is
R5893	T9703	T9678	advcl	is,is
R5894	T9704	T9705	det	the,signal
R5895	T9705	T9703	nsubj	signal,is
R5896	T9706	T9707	advmod	slightly,higher
R5897	T9707	T9703	acomp	higher,is
R5898	T9708	T9709	punct	(,B
R5899	T9709	T9678	parataxis	B,is
R59	T268	T269	aux	To,test
R590	T1195	T1196	det	the,effects
R5900	T9710	T9709	punct	),B
R5901	T9711	T9678	punct	.,is
R5902	T9713	T9714	advmod	Similarly,maintained
R5903	T9715	T9714	punct	", ",maintained
R5904	T9716	T9717	det	the,expression
R5905	T9717	T9714	nsubjpass	expression,maintained
R5906	T9718	T9717	prep	of,expression
R5907	T9719	T9720	compound	Pem,transcripts
R5908	T9720	T9718	pobj	transcripts,of
R5909	T9721	T9714	auxpass	is,maintained
R591	T1196	T1194	dobj	effects,studying
R5910	T9722	T9714	prep	in,maintained
R5911	T9723	T9724	compound	mutant,embryos
R5912	T9724	T9722	pobj	embryos,in
R5913	T9725	T9726	punct	(,D
R5914	T9726	T9724	parataxis	D,embryos
R5915	T9727	T9726	punct	),D
R5916	T9728	T9724	amod	similar,embryos
R5917	T9729	T9728	prep	to,similar
R5918	T9730	T9731	amod	normal,embryos
R5919	T9731	T9729	pobj	embryos,to
R592	T1197	T1196	prep	of,effects
R5920	T9732	T9733	punct	(,C
R5921	T9733	T9731	parataxis	C,embryos
R5922	T9734	T9733	punct	),C
R5923	T9735	T9714	punct	", ",maintained
R5924	T9736	T9737	mark	although,expressed
R5925	T9737	T9714	advcl	expressed,maintained
R5926	T9738	T9737	nsubjpass	Pem,expressed
R5927	T9739	T9737	auxpass	is,expressed
R5928	T9740	T9737	prep	in,expressed
R5929	T9741	T9742	det	the,overhang
R593	T1198	T1199	compound	huntingtin,deficiency
R5930	T9742	T9740	pobj	overhang,in
R5931	T9743	T9742	amod	abnormal,overhang
R5932	T9744	T9742	amod	lopsided,overhang
R5933	T9745	T9742	prep	of,overhang
R5934	T9746	T9747	amod	visceral,endoderm
R5935	T9747	T9745	pobj	endoderm,of
R5936	T9748	T9742	prep	over,overhang
R5937	T9749	T9750	det	the,anterior
R5938	T9750	T9748	pobj	anterior,over
R5939	T9751	T9750	prep	of,anterior
R594	T1199	T1197	pobj	deficiency,of
R5940	T9752	T9753	det	the,embryos
R5941	T9753	T9751	pobj	embryos,of
R5942	T9754	T9753	compound	mutant,embryos
R5943	T9755	T9714	punct	.,maintained
R5944	T9757	T9758	nsubj	Expression,is
R5945	T9759	T9757	prep	of,Expression
R5946	T9760	T9761	amod	extraembryonic,molecules
R5947	T9761	T9759	pobj	molecules,of
R5948	T9762	T9761	amod	signaling,molecules
R5949	T9763	T9758	acomp	unaffected,is
R595	T1200	T1194	prep	in,studying
R5950	T9764	T9763	prep	by,unaffected
R5951	T9765	T9766	det	the,loss
R5952	T9766	T9764	pobj	loss,by
R5953	T9767	T9766	prep	of,loss
R5954	T9768	T9767	pobj	huntingtin,of
R5955	T9769	T9758	punct	", ",is
R5956	T9770	T9771	mark	as,evidenced
R5957	T9771	T9758	advcl	evidenced,is
R5958	T9772	T9771	agent	by,evidenced
R5959	T9773	T9774	det	the,expression
R596	T1201	T1202	det	the,mouse
R5960	T9774	T9772	pobj	expression,by
R5961	T9775	T9774	prep	of,expression
R5962	T9776	T9775	pobj	Bmp4,of
R5963	T9777	T9778	punct	(,F
R5964	T9778	T9776	parataxis	F,Bmp4
R5965	T9779	T9778	nmod	E,F
R5966	T9780	T9778	punct	",",F
R5967	T9781	T9778	punct	),F
R5968	T9782	T9774	prep	in,expression
R5969	T9783	T9784	det	the,ectoderm
R597	T1202	T1200	pobj	mouse,in
R5970	T9784	T9782	pobj	ectoderm,in
R5971	T9785	T9784	amod	extraembryonic,ectoderm
R5972	T9786	T9774	punct	", ",expression
R5973	T9787	T9774	cc	and,expression
R5974	T9788	T9774	conj	Lefty1,expression
R5975	T9789	T9788	cc	and,Lefty1
R5976	T9790	T9788	conj	Dkk1,Lefty1
R5977	T9791	T9792	punct	(,I
R5978	T9792	T9790	parataxis	I,Dkk1
R5979	T9793	T9794	punct	-,L
R598	T1203	T1179	punct	.,set
R5980	T9794	T9792	prep	L,I
R5981	T9795	T9792	punct	),I
R5982	T9796	T9788	prep	in,Lefty1
R5983	T9797	T9798	det	the,AVE
R5984	T9798	T9796	pobj	AVE,in
R5985	T9799	T9774	prep	in,expression
R5986	T9800	T9801	compound	mutant,embryos
R5987	T9801	T9799	pobj	embryos,in
R5988	T9802	T9758	punct	.,is
R5989	T9804	T9805	nsubjpass	Bmp4,localized
R599	T1205	T1206	nsubj	Inactivation,shown
R5990	T9806	T9805	auxpass	is,localized
R5991	T9807	T9805	neg	not,localized
R5992	T9808	T9805	punct	", ",localized
R5993	T9809	T9805	advmod	however,localized
R5994	T9810	T9805	punct	", ",localized
R5995	T9811	T9805	prep	to,localized
R5996	T9812	T9813	det	a,ring
R5997	T9813	T9811	pobj	ring,to
R5998	T9814	T9813	prep	of,ring
R5999	T9815	T9816	amod	extraembryonic,ectoderm
R6	T209	T207	pobj	gene,of
R60	T269	T270	advcl	test,used
R600	T1207	T1205	prep	of,Inactivation
R6000	T9816	T9814	pobj	ectoderm,of
R6001	T9817	T9805	prep	in,localized
R6002	T9818	T9819	compound	mutant,embryos
R6003	T9819	T9817	pobj	embryos,in
R6004	T9820	T9821	punct	(,F
R6005	T9821	T9819	parataxis	F,embryos
R6006	T9822	T9821	punct	),F
R6007	T9823	T9805	prep	as,localized
R6008	T9824	T9823	prep	in,as
R6009	T9825	T9826	amod	normal,embryos
R601	T1208	T1209	det	the,gene
R6010	T9826	T9824	pobj	embryos,in
R6011	T9827	T9828	punct	(,E
R6012	T9828	T9826	parataxis	E,embryos
R6013	T9829	T9828	punct	),E
R6014	T9830	T9805	punct	.,localized
R6015	T9832	T9833	amod	Primitive,cells
R6016	T9833	T9835	nsubjpass	cells,induced
R6017	T9834	T9833	compound	germ,cells
R6018	T9836	T9833	punct	(,cells
R6019	T9837	T9833	appos	PCGs,cells
R602	T1209	T1207	pobj	gene,of
R6020	T9838	T9835	punct	),induced
R6021	T9839	T9835	auxpass	are,induced
R6022	T9840	T9835	advmod	normally,induced
R6023	T9841	T9835	prep	in,induced
R6024	T9842	T9843	preconj	both,type
R6025	T9843	T9846	nmod	type,embryos
R6026	T9844	T9843	amod	wild,type
R6027	T9845	T9843	punct	-,type
R6028	T9846	T9841	pobj	embryos,in
R6029	T9847	T9848	punct	(,G
R603	T1210	T1209	compound	mouse,gene
R6030	T9848	T9843	parataxis	G,type
R6031	T9849	T9848	punct	),G
R6032	T9850	T9843	cc	and,type
R6033	T9851	T9843	conj	mutant,type
R6034	T9852	T9853	punct	(,H
R6035	T9853	T9846	parataxis	H,embryos
R6036	T9854	T9853	punct	),H
R6037	T9855	T9835	punct	", ",induced
R6038	T9856	T9835	advcl	suggesting,induced
R6039	T9857	T9858	det	the,signaling
R604	T1211	T1209	compound	HD,gene
R6040	T9858	T9860	nsubj	signaling,is
R6041	T9859	T9858	compound	Bmp4,signaling
R6042	T9860	T9856	ccomp	is,suggesting
R6043	T9861	T9858	prep	from,signaling
R6044	T9862	T9863	det	the,ectoderm
R6045	T9863	T9861	pobj	ectoderm,from
R6046	T9864	T9863	amod	extraembryonic,ectoderm
R6047	T9865	T9861	prep	to,from
R6048	T9866	T9867	det	the,epiblast
R6049	T9867	T9865	pobj	epiblast,to
R605	T1212	T1209	punct	(,gene
R6050	T9868	T9860	acomp	normal,is
R6051	T9869	T9835	punct	.,induced
R6052	T9871	T9872	compound	Lefty1,expression
R6053	T9872	T9873	nsubj	expression,appears
R6054	T9874	T9873	oprd	disorganized,appears
R6055	T9875	T9873	prep	in,appears
R6056	T9876	T9877	compound	mutant,embryos
R6057	T9877	T9875	pobj	embryos,in
R6058	T9878	T9879	punct	(,I
R6059	T9879	T9877	parataxis	I,embryos
R606	T1213	T1209	appos	Hdh,gene
R6060	T9880	T9879	punct	),I
R6061	T9881	T9873	prep	compared,appears
R6062	T9882	T9881	prep	to,compared
R6063	T9883	T9884	amod	wild,type
R6064	T9884	T9886	compound	type,embryos
R6065	T9885	T9884	punct	-,type
R6066	T9886	T9882	pobj	embryos,to
R6067	T9887	T9888	punct	(,J
R6068	T9888	T9886	parataxis	J,embryos
R6069	T9889	T9888	punct	),J
R607	T1214	T1206	punct	),shown
R6070	T9890	T9873	punct	.,appears
R6071	T9892	T9893	prep	In,matches
R6072	T9894	T9892	pobj	contrast,In
R6073	T9895	T9893	punct	", ",matches
R6074	T9896	T9897	det	the,expression
R6075	T9897	T9893	nsubj	expression,matches
R6076	T9898	T9897	amod	anterior,expression
R6077	T9899	T9897	prep	of,expression
R6078	T9900	T9899	pobj	Dkk1,of
R6079	T9901	T9897	prep	in,expression
R608	T1215	T1206	aux	has,shown
R6080	T9902	T9903	det	the,AVE
R6081	T9903	T9901	pobj	AVE,in
R6082	T9904	T9897	prep	in,expression
R6083	T9905	T9906	compound	mutant,embryos
R6084	T9906	T9904	pobj	embryos,in
R6085	T9907	T9908	punct	(,L
R6086	T9908	T9897	parataxis	L,expression
R6087	T9909	T9908	punct	),L
R6088	T9910	T9911	det	the,pattern
R6089	T9911	T9893	dobj	pattern,matches
R609	T1216	T1217	mark	that,required
R6090	T9912	T9913	amod	wild,type
R6091	T9913	T9911	compound	type,pattern
R6092	T9914	T9913	punct	-,type
R6093	T9915	T9911	compound	expression,pattern
R6094	T9916	T9917	punct	(,K
R6095	T9917	T9911	parataxis	K,pattern
R6096	T9918	T9917	punct	),K
R6097	T9919	T9893	punct	.,matches
R6098	T9921	T9922	prep	Despite,fail
R6099	T9923	T9924	amod	normal,formation
R61	T271	T272	det	these,possibilities
R610	T1217	T1206	ccomp	required,shown
R6100	T9924	T9921	pobj	formation,Despite
R6101	T9925	T9924	compound	AVE,formation
R6102	T9926	T9922	punct	", ",fail
R6103	T9927	T9928	compound	head,folds
R6104	T9928	T9922	nsubj	folds,fail
R6105	T9929	T9930	aux	to,form
R6106	T9930	T9922	xcomp	form,fail
R6107	T9931	T9930	prep	in,form
R6108	T9932	T9933	compound	mutant,embryos
R6109	T9933	T9931	pobj	embryos,in
R611	T1218	T1217	nsubjpass	huntingtin,required
R6110	T9934	T9930	punct	", ",form
R6111	T9935	T9936	advmod	even,cultured
R6112	T9936	T9930	advcl	cultured,form
R6113	T9937	T9936	advmod	when,cultured
R6114	T9938	T9936	prep	in,cultured
R6115	T9939	T9940	npadvmod	nutrient,rich
R6116	T9940	T9941	amod	rich,media
R6117	T9941	T9938	pobj	media,in
R6118	T9942	T9936	prep	for,cultured
R6119	T9943	T9944	nummod	24,hours
R612	T1219	T1217	auxpass	is,required
R6120	T9944	T9942	pobj	hours,for
R6121	T9945	T9922	punct	.,fail
R6122	T9947	T9948	amod	Wild,type
R6123	T9948	T9950	compound	type,embryos
R6124	T9949	T9948	punct	-,type
R6125	T9950	T9952	nsubj	embryos,develop
R6126	T9951	T9950	compound	E7.5,embryos
R6127	T9953	T9952	punct	", ",develop
R6128	T9954	T9955	advmod	when,cultured
R6129	T9955	T9952	advcl	cultured,develop
R613	T1220	T1217	neg	not,required
R6130	T9956	T9955	prep	in,cultured
R6131	T9957	T9958	nummod	75,%
R6132	T9958	T9959	compound	%,serum
R6133	T9959	T9956	pobj	serum,in
R6134	T9960	T9959	compound	rat,serum
R6135	T9961	T9952	punct	", ",develop
R6136	T9962	T9952	dobj	somites,develop
R6137	T9963	T9964	punct	(,M
R6138	T9964	T9962	parataxis	M,somites
R6139	T9965	T9964	punct	),M
R614	T1221	T1217	prep	for,required
R6140	T9966	T9962	punct	", ",somites
R6141	T9967	T9962	conj	heart,somites
R6142	T9968	T9969	punct	(,N
R6143	T9969	T9967	parataxis	N,heart
R6144	T9970	T9971	amod	white,arrow
R6145	T9971	T9969	dep	arrow,N
R6146	T9972	T9969	punct	", ",N
R6147	T9973	T9969	punct	),N
R6148	T9974	T9967	cc	and,heart
R6149	T9975	T9976	compound	head,folds
R615	T1222	T1223	compound	cell,viability
R6150	T9976	T9967	conj	folds,heart
R6151	T9977	T9978	punct	(,N
R6152	T9978	T9976	parataxis	N,folds
R6153	T9979	T9980	amod	blue,head
R6154	T9980	T9978	dep	head,N
R6155	T9981	T9980	compound	arrow,head
R6156	T9982	T9978	punct	", ",N
R6157	T9983	T9978	punct	),N
R6158	T9984	T9952	prep	in,develop
R6159	T9985	T9984	pobj	culture,in
R616	T1223	T1221	pobj	viability,for
R6160	T9986	T9952	punct	.,develop
R6161	T9988	T9989	prep	In,continue
R6162	T9990	T9988	pobj	contrast,In
R6163	T9991	T9989	punct	", ",continue
R6164	T9992	T9993	npadvmod	huntingtin,deficient
R6165	T9993	T9994	amod	deficient,embryos
R6166	T9994	T9989	nsubj	embryos,continue
R6167	T9995	T9996	aux	to,live
R6168	T9996	T9989	xcomp	live,continue
R6169	T9997	T9996	prep	in,live
R617	T1224	T1217	punct	", ",required
R6170	T9998	T9997	pobj	culture,in
R6171	T9999	T9989	cc	but,continue
R6172	T10000	T10001	aux	do,form
R6173	T10001	T9989	conj	form,continue
R6174	T10002	T10001	neg	not,form
R6175	T10003	T10001	dobj	headfolds,form
R6176	T10004	T10003	punct	", ",headfolds
R6177	T10005	T10003	conj	heart,headfolds
R6178	T10006	T10005	cc	or,heart
R6179	T10007	T10005	conj	somites,heart
R618	T1225	T1226	mark	as,evidenced
R6180	T10008	T10009	punct	(,O
R6181	T10009	T10007	parataxis	O,somites
R6182	T10010	T10009	punct	),O
R6183	T10011	T9989	punct	.,continue
R6184	T10013	T10014	nsubjpass	Embryos,shown
R6185	T10015	T10014	auxpass	are,shown
R6186	T10016	T10014	prep	in,shown
R6187	T10017	T10018	det	a,view
R6188	T10018	T10016	pobj	view,in
R6189	T10019	T10018	amod	lateral,view
R619	T1226	T1217	advcl	evidenced,required
R6190	T10020	T10021	punct	(,I
R6191	T10021	T10014	parataxis	I,shown
R6192	T10022	T10021	dep	A,I
R6193	T10023	T10024	punct	-,F
R6194	T10024	T10022	prep	F,A
R6195	T10025	T10021	punct	", ",I
R6196	T10026	T10027	punct	-,J
R6197	T10027	T10021	prep	J,I
R6198	T10028	T10021	punct	),I
R6199	T10029	T10030	mark	with,oriented
R62	T272	T269	dobj	possibilities,test
R620	T1227	T1226	agent	by,evidenced
R6200	T10030	T10014	advcl	oriented,shown
R6201	T10031	T10030	nsubj	anterior,oriented
R6202	T10032	T10030	prep	to,oriented
R6203	T10033	T10034	det	the,left
R6204	T10034	T10032	pobj	left,to
R6205	T10035	T10014	punct	.,shown
R6206	T10037	T10038	nsubjpass	Embryos,shown
R6207	T10039	T10037	prep	in,Embryos
R6208	T10040	T10039	punct	(,in
R6209	T10041	T10042	nmod	G,L
R621	T1228	T1229	det	the,survival
R6210	T10042	T10039	pobj	L,in
R6211	T10043	T10042	punct	",",L
R6212	T10044	T10042	nmod	H,L
R6213	T10045	T10042	punct	",",L
R6214	T10046	T10042	nmod	K,L
R6215	T10047	T10042	punct	",",L
R6216	T10048	T10038	punct	),shown
R6217	T10049	T10038	auxpass	are,shown
R6218	T10050	T10038	prep	in,shown
R6219	T10051	T10052	det	an,view
R622	T1229	T1227	pobj	survival,by
R6220	T10052	T10050	pobj	view,in
R6221	T10053	T10052	amod	anterior,view
R6222	T10054	T10055	mark	with,oriented
R6223	T10055	T10038	advcl	oriented,shown
R6224	T10056	T10055	nsubj	proximal,oriented
R6225	T10057	T10055	advmod	up,oriented
R6226	T10058	T10038	punct	.,shown
R623	T1230	T1229	prep	of,survival
R624	T1231	T1232	nmod	mouse,cells
R625	T1232	T1230	pobj	cells,of
R626	T1233	T1232	amod	embryonic,cells
R627	T1234	T1232	compound	stem,cells
R628	T1235	T1232	cc	and,cells
R629	T1236	T1232	conj	neurons,cells
R63	T273	T270	punct	", ",used
R630	T1237	T1238	dep	that,lack
R631	T1238	T1236	relcl	lack,neurons
R632	T1239	T1238	dobj	huntingtin,lack
R633	T1240	T1241	punct	[,10
R634	T1241	T1226	parataxis	10,evidenced
R635	T1242	T1243	punct	-,12
R636	T1243	T1241	prep	12,10
R637	T1244	T1241	punct	],10
R638	T1245	T1206	punct	.,shown
R639	T1247	T1248	advmod	However,needed
R64	T274	T270	nsubj	we,used
R640	T1249	T1248	punct	", ",needed
R641	T1250	T1248	nsubjpass	huntingtin,needed
R642	T1251	T1248	auxpass	is,needed
R643	T1252	T1248	prep	at,needed
R644	T1253	T1254	det	the,level
R645	T1254	T1252	pobj	level,at
R646	T1255	T1254	prep	of,level
R647	T1256	T1257	det	the,organism
R648	T1257	T1255	pobj	organism,of
R649	T1258	T1248	prep	for,needed
R65	T275	T270	aux	have,used
R650	T1259	T1260	amod	proper,development
R651	T1260	T1258	pobj	development,for
R652	T1261	T1260	amod	mammalian,development
R653	T1262	T1260	amod	embryonic,development
R654	T1263	T1264	punct	[,14
R655	T1264	T1248	parataxis	14,needed
R656	T1265	T1264	nummod	10,14
R657	T1266	T1264	punct	",",14
R658	T1267	T1264	nummod	13,14
R659	T1268	T1264	punct	",",14
R66	T276	T277	amod	whole,mount
R660	T1269	T1264	punct	],14
R661	T1270	T1248	punct	.,needed
R662	T1272	T1273	amod	Complete,lack
R663	T1273	T1274	nsubj	lack,results
R664	T1275	T1273	prep	of,lack
R665	T1276	T1275	pobj	huntingtin,of
R666	T1277	T1274	prep	in,results
R667	T1278	T1279	amod	developmental,arrest
R668	T1279	T1277	pobj	arrest,in
R669	T1280	T1274	prep	during,results
R67	T277	T278	nmod	mount,hybridization
R670	T1281	T1280	pobj	gastrulation,during
R671	T1282	T1274	punct	", ",results
R672	T1283	T1284	mark	while,results
R673	T1284	T1274	advcl	results,results
R674	T1285	T1286	amod	severe,reduction
R675	T1286	T1284	nsubj	reduction,results
R676	T1287	T1286	prep	of,reduction
R677	T1288	T1289	compound	huntingtin,levels
R678	T1289	T1287	pobj	levels,of
R679	T1290	T1284	prep	in,results
R68	T278	T270	dobj	hybridization,used
R680	T1291	T1292	amod	abnormal,neurogenesis
R681	T1292	T1290	pobj	neurogenesis,in
R682	T1293	T1292	cc	and,neurogenesis
R683	T1294	T1295	amod	perinatal,lethality
R684	T1295	T1292	conj	lethality,neurogenesis
R685	T1296	T1297	punct	[,15
R686	T1297	T1284	parataxis	15,results
R687	T1298	T1297	punct	],15
R688	T1299	T1274	punct	.,results
R689	T1301	T1302	nsubj	Analysis,reveals
R69	T279	T280	advmod	in,situ
R690	T1303	T1301	prep	of,Analysis
R691	T1304	T1305	npadvmod	huntingtin,deficient
R692	T1305	T1306	amod	deficient,embryos
R693	T1306	T1303	pobj	embryos,of
R694	T1307	T1306	nmod	Hdhex4,embryos
R695	T1308	T1307	punct	/,Hdhex4
R696	T1309	T1307	nummod	5,Hdhex4
R697	T1310	T1307	punct	/,Hdhex4
R698	T1311	T1307	appos	Hdhex4,Hdhex4
R699	T1312	T1311	punct	/,Hdhex4
R7	T210	T211	poss	Huntington,disease
R70	T280	T278	amod	situ,hybridization
R700	T1313	T1311	nummod	5,Hdhex4
R701	T1314	T1315	mark	that,affect
R702	T1315	T1302	ccomp	affect,reveals
R703	T1316	T1317	amod	homozygous,inactivation
R704	T1317	T1315	nsubj	inactivation,affect
R705	T1318	T1317	prep	of,inactivation
R706	T1319	T1320	det	the,gene
R707	T1320	T1318	pobj	gene,of
R708	T1321	T1320	compound	mouse,gene
R709	T1322	T1320	compound	HD,gene
R71	T281	T282	aux	to,examine
R710	T1323	T1315	aux	does,affect
R711	T1324	T1315	neg	not,affect
R712	T1325	T1315	advmod	overtly,affect
R713	T1326	T1315	dobj	development,affect
R714	T1327	T1315	prep	until,affect
R715	T1328	T1327	pobj	E7.0,until
R716	T1329	T1302	punct	.,reveals
R717	T1331	T1332	prep	By,exhibit
R718	T1333	T1331	pobj	E7.5,By
R719	T1334	T1332	punct	", ",exhibit
R72	T282	T270	advcl	examine,used
R720	T1335	T1336	compound	mutant,embryos
R721	T1336	T1332	nsubj	embryos,exhibit
R722	T1337	T1338	det	a,streak
R723	T1338	T1332	dobj	streak,exhibit
R724	T1339	T1338	amod	shortened,streak
R725	T1340	T1338	amod	primitive,streak
R726	T1341	T1338	punct	", ",streak
R727	T1342	T1343	amod	reduced,size
R728	T1343	T1338	appos	size,streak
R729	T1344	T1332	cc	and,exhibit
R73	T283	T284	amod	embryonic,patterning
R730	T1345	T1332	punct	", ",exhibit
R731	T1346	T1347	prep	by,lack
R732	T1347	T1332	conj	lack,exhibit
R733	T1348	T1346	pobj	morphology,by
R734	T1349	T1347	punct	", ",lack
R735	T1350	T1351	det	a,node
R736	T1351	T1347	dobj	node,lack
R737	T1352	T1351	cc	and,node
R738	T1353	T1354	compound	head,folds
R739	T1354	T1351	conj	folds,node
R74	T284	T282	dobj	patterning,examine
R740	T1355	T1332	punct	.,exhibit
R741	T1357	T1358	nsubjpass	Mutants,resorbed
R742	T1359	T1358	auxpass	are,resorbed
R743	T1360	T1358	advmod	rapidly,resorbed
R744	T1361	T1358	prep	by,resorbed
R745	T1362	T1361	pobj	E8.0,by
R746	T1363	T1364	punct	[,10
R747	T1364	T1358	parataxis	10,resorbed
R748	T1365	T1364	punct	],10
R749	T1366	T1358	punct	.,resorbed
R75	T285	T284	cc	and,patterning
R750	T1368	T1369	advmod	Importantly,rescues
R751	T1370	T1369	punct	", ",rescues
R752	T1371	T1372	det	the,expression
R753	T1372	T1369	nsubj	expression,rescues
R754	T1373	T1372	prep	of,expression
R755	T1374	T1373	pobj	huntingtin,of
R756	T1375	T1376	advmod	only,in
R757	T1376	T1372	prep	in,expression
R758	T1377	T1378	amod	extraembryonic,tissues
R759	T1378	T1376	pobj	tissues,in
R76	T286	T284	conj	morphogenesis,patterning
R760	T1379	T1372	prep	in,expression
R761	T1380	T1379	pobj	chimeras,in
R762	T1381	T1382	det	this,phenotype
R763	T1382	T1369	dobj	phenotype,rescues
R764	T1383	T1382	compound	gastrulation,phenotype
R765	T1384	T1369	punct	", ",rescues
R766	T1385	T1369	advcl	suggesting,rescues
R767	T1386	T1387	mark	that,required
R768	T1387	T1385	ccomp	required,suggesting
R769	T1388	T1387	nsubjpass	huntingtin,required
R77	T287	T282	prep	in,examine
R770	T1389	T1387	auxpass	is,required
R771	T1390	T1391	advmod	only,in
R772	T1391	T1387	prep	in,required
R773	T1392	T1391	pobj	cells,in
R774	T1393	T1392	prep	of,cells
R775	T1394	T1395	det	the,lineage
R776	T1395	T1393	pobj	lineage,of
R777	T1396	T1395	amod	extraembryonic,lineage
R778	T1397	T1387	cc	and,required
R779	T1398	T1387	conj	acts,required
R78	T288	T289	amod	homozygous,embryos
R780	T1399	T1398	prep	in,acts
R781	T1400	T1401	det	a,manner
R782	T1401	T1399	pobj	manner,in
R783	T1402	T1403	npadvmod	cell,non-autonomous
R784	T1403	T1401	amod	non-autonomous,manner
R785	T1404	T1398	prep	at,acts
R786	T1405	T1406	det	this,stage
R787	T1406	T1404	pobj	stage,at
R788	T1407	T1408	punct	[,16
R789	T1408	T1398	parataxis	16,acts
R79	T289	T287	pobj	embryos,in
R790	T1409	T1408	punct	],16
R791	T1410	T1369	punct	.,rescues
R792	T1412	T1413	amod	Extraembryonic,tissues
R793	T1413	T1414	nsubj	tissues,are
R794	T1415	T1414	acomp	essential,are
R795	T1416	T1415	prep	for,essential
R796	T1417	T1416	pcomp	supplying,for
R797	T1418	T1417	dobj	nutrients,supplying
R798	T1419	T1418	cc	and,nutrients
R799	T1420	T1418	conj	signals,nutrients
R8	T211	T209	compound	disease,gene
R80	T290	T289	nmod	Hdhex4,embryos
R800	T1421	T1422	dep	that,direct
R801	T1422	T1418	relcl	direct,nutrients
R802	T1423	T1424	amod	anterior,posterior
R803	T1424	T1426	amod	posterior,formation
R804	T1425	T1424	punct	/,posterior
R805	T1426	T1422	dobj	formation,direct
R806	T1427	T1426	compound	axis,formation
R807	T1428	T1426	cc	and,formation
R808	T1429	T1426	conj	patterning,formation
R809	T1430	T1422	prep	in,direct
R81	T291	T290	punct	/,Hdhex4
R810	T1431	T1432	det	the,embryo
R811	T1432	T1430	pobj	embryo,in
R812	T1433	T1432	amod	developing,embryo
R813	T1434	T1435	punct	(,reviewed
R814	T1435	T1422	parataxis	reviewed,direct
R815	T1436	T1435	prep	in,reviewed
R816	T1437	T1436	punct	[,in
R817	T1438	T1436	pobj	17,in
R818	T1439	T1435	punct	],reviewed
R819	T1440	T1435	punct	),reviewed
R82	T292	T290	nummod	5,Hdhex4
R820	T1441	T1422	punct	", ",direct
R821	T1442	T1422	advcl	implicating,direct
R822	T1443	T1442	dobj	huntingtin,implicating
R823	T1444	T1442	prep	in,implicating
R824	T1445	T1444	pobj	either,in
R825	T1446	T1445	cc	or,either
R826	T1447	T1445	conj	both,either
R827	T1448	T1445	prep	of,either
R828	T1449	T1450	det	these,processes
R829	T1450	T1448	pobj	processes,of
R83	T293	T294	npadvmod	huntingtin,deficient
R830	T1451	T1414	punct	.,are
R831	T1453	T1454	prep	Of,investigated
R832	T1455	T1456	det	these,possibilities
R833	T1456	T1453	pobj	possibilities,Of
R834	T1457	T1454	punct	", ",investigated
R835	T1458	T1459	det	the,role
R836	T1459	T1454	nsubjpass	role,investigated
R837	T1460	T1459	amod	nutritive,role
R838	T1461	T1454	aux	has,investigated
R839	T1462	T1454	auxpass	been,investigated
R84	T294	T289	amod	deficient,embryos
R840	T1463	T1464	advmod	more,extensively
R841	T1464	T1454	advmod	extensively,investigated
R842	T1465	T1454	punct	.,investigated
R843	T1467	T1468	advmod	However,display
R844	T1469	T1468	punct	", ",display
R845	T1470	T1471	npadvmod	huntingtin,deficient
R846	T1471	T1472	amod	deficient,embryos
R847	T1472	T1468	nsubj	embryos,display
R848	T1473	T1468	aux	do,display
R849	T1474	T1468	neg	not,display
R85	T295	T270	punct	.,used
R850	T1475	T1476	amod	obvious,defects
R851	T1476	T1468	dobj	defects,display
R852	T1477	T1476	amod	visceral,defects
R853	T1478	T1476	compound	endoderm,defects
R854	T1479	T1468	punct	", ",display
R855	T1480	T1468	prep	with,display
R856	T1481	T1482	det	the,exception
R857	T1482	T1480	pobj	exception,with
R858	T1483	T1482	amod	notable,exception
R859	T1484	T1482	prep	of,exception
R86	T299	T300	prep	In,appears
R860	T1485	T1486	amod	compromised,transport
R861	T1486	T1484	pobj	transport,of
R862	T1487	T1486	compound	iron,transport
R863	T1488	T1486	prep	in,transport
R864	T1489	T1490	amod	later,mutants
R865	T1490	T1488	pobj	mutants,in
R866	T1491	T1490	compound	stage,mutants
R867	T1492	T1468	punct	", ",display
R868	T1493	T1494	mark	although,is
R869	T1494	T1468	advcl	is,display
R87	T301	T302	det	the,absence
R870	T1495	T1496	compound	iron,uptake
R871	T1496	T1494	nsubj	uptake,is
R872	T1497	T1494	acomp	undisturbed,is
R873	T1498	T1499	punct	[,16
R874	T1499	T1494	parataxis	16,is
R875	T1500	T1499	punct	],16
R876	T1501	T1494	cc	and,is
R877	T1502	T1503	nsubjpass	endocytosis,impaired
R878	T1503	T1494	conj	impaired,is
R879	T1504	T1503	auxpass	is,impaired
R88	T302	T299	pobj	absence,In
R880	T1505	T1503	neg	not,impaired
R881	T1506	T1503	prep	in,impaired
R882	T1507	T1508	npadvmod	huntingtin,deficient
R883	T1508	T1509	amod	deficient,embryos
R884	T1509	T1506	pobj	embryos,in
R885	T1510	T1509	cc	or,embryos
R886	T1511	T1512	amod	embryonic,cells
R887	T1512	T1509	conj	cells,embryos
R888	T1513	T1512	compound	stem,cells
R889	T1514	T1515	punct	[,18
R89	T303	T302	prep	of,absence
R890	T1515	T1503	parataxis	18,impaired
R891	T1516	T1515	nummod	16,18
R892	T1517	T1515	punct	",",18
R893	T1518	T1515	punct	],18
R894	T1519	T1468	punct	.,display
R895	T1521	T1522	prep	By,shuttles
R896	T1523	T1524	det	the,token
R897	T1524	T1521	pobj	token,By
R898	T1525	T1524	amod	same,token
R899	T1526	T1522	punct	", ",shuttles
R9	T212	T210	case	's,Huntington
R90	T304	T303	pobj	huntingtin,of
R900	T1527	T1522	nsubj	huntingtin,shuttles
R901	T1528	T1522	prep	through,shuttles
R902	T1529	T1530	det	the,nucleus
R903	T1530	T1528	pobj	nucleus,through
R904	T1531	T1530	punct	", ",nucleus
R905	T1532	T1533	advmod	where,required
R906	T1533	T1530	relcl	required,nucleus
R907	T1534	T1533	nsubjpass	it,required
R908	T1535	T1533	auxpass	is,required
R909	T1536	T1533	prep	for,required
R91	T305	T300	punct	", ",appears
R910	T1537	T1538	amod	proper,localization
R911	T1538	T1536	pobj	localization,for
R912	T1539	T1538	amod	nuclear,localization
R913	T1540	T1538	prep	of,localization
R914	T1541	T1542	poss	its,partners
R915	T1542	T1540	pobj	partners,of
R916	T1543	T1544	compound	transcription,factor
R917	T1544	T1542	compound	factor,partners
R918	T1545	T1533	punct	", ",required
R919	T1546	T1533	advcl	suggesting,required
R92	T306	T300	nsubj	expression,appears
R920	T1547	T1548	mark	that,play
R921	T1548	T1546	ccomp	play,suggesting
R922	T1549	T1548	nsubj	huntingtin,play
R923	T1550	T1548	aux	may,play
R924	T1551	T1552	det	a,role
R925	T1552	T1548	dobj	role,play
R926	T1553	T1548	prep	in,play
R927	T1554	T1555	compound	transcription,cascades
R928	T1555	T1553	pobj	cascades,in
R929	T1556	T1548	prep	in,play
R93	T307	T306	prep	of,expression
R930	T1557	T1558	amod	extraembryonic,tissues
R931	T1558	T1556	pobj	tissues,in
R932	T1559	T1560	dep	that,pattern
R933	T1560	T1558	relcl	pattern,tissues
R934	T1561	T1562	det	the,embryo
R935	T1562	T1560	dobj	embryo,pattern
R936	T1563	T1564	punct	[,18
R937	T1564	T1546	parataxis	18,suggesting
R938	T1565	T1564	punct	],18
R939	T1566	T1522	punct	.,shuttles
R94	T308	T309	amod	nutritive,genes
R940	T1568	T1569	advmod	Therefore,examined
R941	T1570	T1569	punct	", ",examined
R942	T1571	T1569	nsubj	we,examined
R943	T1572	T1569	aux	have,examined
R944	T1573	T1574	det	this,hypothesis
R945	T1574	T1569	dobj	hypothesis,examined
R946	T1575	T1569	punct	", ",examined
R947	T1576	T1569	prep	by,examined
R948	T1577	T1576	pcomp	monitoring,by
R949	T1578	T1579	det	the,expression
R95	T309	T307	pobj	genes,of
R950	T1579	T1577	dobj	expression,monitoring
R951	T1580	T1579	prep	of,expression
R952	T1581	T1580	pobj	genes,of
R953	T1582	T1583	dep	that,determine
R954	T1583	T1581	relcl	determine,genes
R955	T1584	T1585	amod	normal,patterning
R956	T1585	T1583	dobj	patterning,determine
R957	T1586	T1585	amod	embryonic,patterning
R958	T1587	T1585	cc	and,patterning
R959	T1588	T1585	conj	morphogenesis,patterning
R96	T310	T300	oprd	normal,appears
R960	T1589	T1583	prep	in,determine
R961	T1590	T1591	nmod	Hdhex4,embryos
R962	T1591	T1589	pobj	embryos,in
R963	T1592	T1590	punct	/,Hdhex4
R964	T1593	T1590	nummod	5,Hdhex4
R965	T1594	T1590	punct	/,Hdhex4
R966	T1595	T1590	appos	Hdhex4,Hdhex4
R967	T1596	T1595	punct	/,Hdhex4
R968	T1597	T1595	nummod	5,Hdhex4
R969	T1598	T1599	npadvmod	huntingtin,deficient
R97	T311	T300	cc	but,appears
R970	T1599	T1591	amod	deficient,embryos
R971	T1600	T1569	punct	.,examined
R972	T1602	T1603	poss	Our,results
R973	T1603	T1604	nsubj	results,support
R974	T1605	T1604	cc	and,support
R975	T1606	T1604	conj	refine,support
R976	T1607	T1608	det	the,hypothesis
R977	T1608	T1606	dobj	hypothesis,refine
R978	T1609	T1606	punct	", ",refine
R979	T1610	T1606	conj	indicating,refine
R98	T312	T313	nmod	E7.0,embryos
R980	T1611	T1612	mark	that,required
R981	T1612	T1610	ccomp	required,indicating
R982	T1613	T1612	nsubjpass	huntingtin,required
R983	T1614	T1612	auxpass	is,required
R984	T1615	T1612	prep	for,required
R985	T1616	T1617	amod	proper,patterning
R986	T1617	T1615	pobj	patterning,for
R987	T1618	T1617	compound	mesoderm,patterning
R988	T1619	T1615	cc	and,for
R989	T1620	T1615	conj	for,for
R99	T313	T316	nsubj	embryos,exhibit
R990	T1621	T1622	amod	normal,restriction
R991	T1622	T1620	pobj	restriction,for
R992	T1623	T1622	amod	regional,restriction
R993	T1624	T1622	prep	of,restriction
R994	T1625	T1626	det	the,expression
R995	T1626	T1624	pobj	expression,of
R996	T1627	T1626	prep	of,expression
R997	T1628	T1629	det	a,subset
R998	T1629	T1627	pobj	subset,of
R999	T1630	T1629	prep	of,subset

PMC:1208873 JSONTXT 7 Projects

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PMC:1208873 JSON TXT 7 Projects