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PMC:1189074 / 4340-16229 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T964 9-13 NN denotes Gene
T965 14-23 NN denotes Targeting
T966 24-27 CC denotes and
T967 28-38 NN denotes Generation
T968 39-41 IN denotes of
T969 42-46 NN denotes MCAD
T971 46-47 HYPH denotes -
T970 47-56 JJ denotes Deficient
T972 57-61 NNS denotes Mice
T973 61-228 sentence denotes MCAD insertion vector (MCAD IV2) was designed to undergo gap repair of the 1.3-kb deleted region upon homologous recombination in 129P2 (129P2/OlaHsd) ES cells E14–1.
T974 62-66 NN denotes MCAD
T976 67-76 NN denotes insertion
T975 77-83 NN denotes vector
T978 84-85 -LRB- denotes (
T979 85-89 NN denotes MCAD
T980 90-93 NN denotes IV2
T981 93-94 -RRB- denotes )
T982 95-98 VBD denotes was
T977 99-107 VBN denotes designed
T983 108-110 TO denotes to
T984 111-118 VB denotes undergo
T985 119-122 NN denotes gap
T986 123-129 NN denotes repair
T987 130-132 IN denotes of
T988 133-136 DT denotes the
T990 137-140 CD denotes 1.3
T992 140-141 HYPH denotes -
T991 141-143 NN denotes kb
T993 144-151 VBN denotes deleted
T989 152-158 NN denotes region
T994 159-163 IN denotes upon
T995 164-174 JJ denotes homologous
T996 175-188 NN denotes recombination
T997 189-191 IN denotes in
T998 192-197 NN denotes 129P2
T1000 198-199 -LRB- denotes (
T1002 199-204 NN denotes 129P2
T1003 204-205 HYPH denotes /
T1001 205-211 NN denotes OlaHsd
T1004 211-212 -RRB- denotes )
T1005 213-215 NN denotes ES
T999 216-221 NNS denotes cells
T1006 222-225 NN denotes E14
T1007 225-226 HYPH denotes –
T1008 226-227 CD denotes 1
T1009 227-228 . denotes .
T1010 228-379 sentence denotes Correct targeting of the MCAD locus resulted in a duplication of exons 8, 9, and 10 and integration of flanking plasmid and Neo sequences (Figure 1A).
T1011 229-236 JJ denotes Correct
T1012 237-246 NN denotes targeting
T1014 247-249 IN denotes of
T1015 250-253 DT denotes the
T1017 254-258 NN denotes MCAD
T1016 259-264 NN denotes locus
T1013 265-273 VBD denotes resulted
T1018 274-276 IN denotes in
T1019 277-278 DT denotes a
T1020 279-290 NN denotes duplication
T1021 291-293 IN denotes of
T1022 294-299 NNS denotes exons
T1023 300-301 CD denotes 8
T1024 301-303 , denotes ,
T1025 303-304 CD denotes 9
T1026 304-306 , denotes ,
T1027 306-309 CC denotes and
T1028 310-312 CD denotes 10
T1029 313-316 CC denotes and
T1030 317-328 NN denotes integration
T1031 329-331 IN denotes of
T1032 332-340 VBG denotes flanking
T1033 341-348 NN denotes plasmid
T1034 349-352 CC denotes and
T1035 353-356 NN denotes Neo
T1036 357-366 NNS denotes sequences
T1037 367-368 -LRB- denotes (
T1038 368-374 NN denotes Figure
T1039 375-377 CD denotes 1A
T1040 377-378 -RRB- denotes )
T1041 378-379 . denotes .
T1042 379-463 sentence denotes The insertion vector was designed to duplicate exon 8, 9, and 10 at the MCAD locus.
T1043 380-383 DT denotes The
T1045 384-393 NN denotes insertion
T1044 394-400 NN denotes vector
T1047 401-404 VBD denotes was
T1046 405-413 VBN denotes designed
T1048 414-416 TO denotes to
T1049 417-426 VB denotes duplicate
T1050 427-431 NN denotes exon
T1051 432-433 CD denotes 8
T1052 433-435 , denotes ,
T1053 435-436 CD denotes 9
T1054 436-438 , denotes ,
T1055 438-441 CC denotes and
T1056 442-444 CD denotes 10
T1057 445-447 IN denotes at
T1058 448-451 DT denotes the
T1060 452-456 NN denotes MCAD
T1059 457-462 NN denotes locus
T1061 462-463 . denotes .
T1062 463-602 sentence denotes Translation of the duplicated exon 8 region results in the formation of premature stop codons resulting in truncation of the MCAD monomer.
T1063 464-475 NN denotes Translation
T1065 476-478 IN denotes of
T1066 479-482 DT denotes the
T1068 483-493 VBN denotes duplicated
T1069 494-498 NN denotes exon
T1070 499-500 CD denotes 8
T1067 501-507 NN denotes region
T1064 508-515 VBZ denotes results
T1071 516-518 IN denotes in
T1072 519-522 DT denotes the
T1073 523-532 NN denotes formation
T1074 533-535 IN denotes of
T1075 536-545 JJ denotes premature
T1077 546-550 NN denotes stop
T1076 551-557 NNS denotes codons
T1078 558-567 VBG denotes resulting
T1079 568-570 IN denotes in
T1080 571-581 NN denotes truncation
T1081 582-584 IN denotes of
T1082 585-588 DT denotes the
T1084 589-593 NN denotes MCAD
T1083 594-601 NN denotes monomer
T1085 601-602 . denotes .
T1086 602-726 sentence denotes Specifically, the first premature stop codon arises after translation of only seven amino acids from the duplicated exon 8.
T1087 603-615 RB denotes Specifically
T1089 615-617 , denotes ,
T1090 617-620 DT denotes the
T1092 621-626 JJ denotes first
T1093 627-636 JJ denotes premature
T1094 637-641 NN denotes stop
T1091 642-647 NN denotes codon
T1088 648-654 VBZ denotes arises
T1095 655-660 IN denotes after
T1096 661-672 NN denotes translation
T1097 673-675 IN denotes of
T1098 676-680 RB denotes only
T1099 681-686 CD denotes seven
T1101 687-692 NN denotes amino
T1100 693-698 NNS denotes acids
T1102 699-703 IN denotes from
T1103 704-707 DT denotes the
T1105 708-718 VBN denotes duplicated
T1104 719-723 NN denotes exon
T1106 724-725 CD denotes 8
T1107 725-726 . denotes .
T1108 726-896 sentence denotes The resulting MCAD monomer is missing the C-terminal domain α-helixes that are responsible for making intersubunit contacts to generate the functional MCAD homotetramer.
T1109 727-730 DT denotes The
T1111 731-740 VBG denotes resulting
T1112 741-745 NN denotes MCAD
T1110 746-753 NN denotes monomer
T1114 754-756 VBZ denotes is
T1113 757-764 VBG denotes missing
T1115 765-768 DT denotes the
T1117 769-770 NN denotes C
T1119 770-771 HYPH denotes -
T1118 771-779 JJ denotes terminal
T1120 780-786 NN denotes domain
T1121 787-788 NN denotes α
T1122 788-789 HYPH denotes -
T1116 789-796 NNS denotes helixes
T1123 797-801 WDT denotes that
T1124 802-805 VBP denotes are
T1125 806-817 JJ denotes responsible
T1126 818-821 IN denotes for
T1127 822-828 VBG denotes making
T1128 829-841 NN denotes intersubunit
T1129 842-850 NNS denotes contacts
T1130 851-853 TO denotes to
T1131 854-862 VB denotes generate
T1132 863-866 DT denotes the
T1134 867-877 JJ denotes functional
T1135 878-882 NN denotes MCAD
T1133 883-895 NN denotes homotetramer
T1136 895-896 . denotes .
T1137 896-1720 sentence denotes Figure 1 Strategy for Disruption of the Mouse Acadm Gene (A) The MCAD IV2 insertion targeting vector with a deleted 1.3-kb region encompassing exon 10 and flanking sequences. MCAD IV2 undergoes gap repair upon homologous recombination at the endogenous Acadm locus resulting in a duplication of exons 8, 9, and 10 at the disrupted allele. (B) Southern blot analysis of EcoRI-digested genomic DNA from ES cells screened by PCR. Probe A, a DNA fragment consisting of a portion of exon 10 that is not present in the targeting vector, hybridizes to an endogenous 3.1-kb fragment and, upon homologous recombination, to a 13.2-kb fragment. Lane 1 represents a wild-type ES cell line, and Lane 2 and 3 represent targeted ES cell lines. ES cell clones were screened by PCR (data not shown) and confirmed by Southern blot analysis.
T7247 907-915 NN denotes Strategy
T7248 916-919 IN denotes for
T7249 920-930 NN denotes Disruption
T7250 931-933 IN denotes of
T7251 934-937 DT denotes the
T7253 938-943 NN denotes Mouse
T7254 944-949 NN denotes Acadm
T7252 950-954 NN denotes Gene
T7255 954-1072 sentence denotes (A) The MCAD IV2 insertion targeting vector with a deleted 1.3-kb region encompassing exon 10 and flanking sequences.
T7256 955-956 -LRB- denotes (
T7257 956-957 LS denotes A
T7259 957-958 -RRB- denotes )
T7260 959-962 DT denotes The
T7261 963-967 NN denotes MCAD
T7263 968-971 NN denotes IV2
T7262 972-981 NN denotes insertion
T7264 982-991 NN denotes targeting
T7258 992-998 NN denotes vector
T7265 999-1003 IN denotes with
T7266 1004-1005 DT denotes a
T7268 1006-1013 VBN denotes deleted
T7269 1014-1017 CD denotes 1.3
T7271 1017-1018 HYPH denotes -
T7270 1018-1020 NN denotes kb
T7267 1021-1027 NN denotes region
T7272 1028-1040 VBG denotes encompassing
T7273 1041-1045 NN denotes exon
T7274 1046-1048 CD denotes 10
T7275 1049-1052 CC denotes and
T7276 1053-1061 VBG denotes flanking
T7277 1062-1071 NNS denotes sequences
T7278 1071-1072 . denotes .
T7279 1072-1236 sentence denotes MCAD IV2 undergoes gap repair upon homologous recombination at the endogenous Acadm locus resulting in a duplication of exons 8, 9, and 10 at the disrupted allele.
T7280 1073-1077 NN denotes MCAD
T7281 1078-1081 NN denotes IV2
T7282 1082-1091 VBZ denotes undergoes
T7283 1092-1095 NN denotes gap
T7284 1096-1102 NN denotes repair
T7285 1103-1107 IN denotes upon
T7286 1108-1118 JJ denotes homologous
T7287 1119-1132 NN denotes recombination
T7288 1133-1135 IN denotes at
T7289 1136-1139 DT denotes the
T7291 1140-1150 JJ denotes endogenous
T7292 1151-1156 NN denotes Acadm
T7290 1157-1162 NN denotes locus
T7293 1163-1172 VBG denotes resulting
T7294 1173-1175 IN denotes in
T7295 1176-1177 DT denotes a
T7296 1178-1189 NN denotes duplication
T7297 1190-1192 IN denotes of
T7298 1193-1198 NNS denotes exons
T7299 1199-1200 CD denotes 8
T7300 1200-1202 , denotes ,
T7301 1202-1203 CD denotes 9
T7302 1203-1205 , denotes ,
T7303 1205-1208 CC denotes and
T7304 1209-1211 CD denotes 10
T7305 1212-1214 IN denotes at
T7306 1215-1218 DT denotes the
T7308 1219-1228 VBN denotes disrupted
T7307 1229-1235 NN denotes allele
T7309 1235-1236 . denotes .
T7310 1236-1324 sentence denotes (B) Southern blot analysis of EcoRI-digested genomic DNA from ES cells screened by PCR.
T7311 1237-1238 -LRB- denotes (
T7312 1238-1239 LS denotes B
T7314 1239-1240 -RRB- denotes )
T7315 1241-1249 NNP denotes Southern
T7316 1250-1254 NN denotes blot
T7313 1255-1263 NN denotes analysis
T7317 1264-1266 IN denotes of
T7318 1267-1272 NN denotes EcoRI
T7320 1272-1273 HYPH denotes -
T7319 1273-1281 VBN denotes digested
T7322 1282-1289 JJ denotes genomic
T7321 1290-1293 NN denotes DNA
T7323 1294-1298 IN denotes from
T7324 1299-1301 NN denotes ES
T7325 1302-1307 NNS denotes cells
T7326 1308-1316 VBN denotes screened
T7327 1317-1319 IN denotes by
T7328 1320-1323 NN denotes PCR
T7329 1323-1324 . denotes .
T7330 1324-1531 sentence denotes Probe A, a DNA fragment consisting of a portion of exon 10 that is not present in the targeting vector, hybridizes to an endogenous 3.1-kb fragment and, upon homologous recombination, to a 13.2-kb fragment.
T7331 1325-1330 NN denotes Probe
T7332 1331-1332 NN denotes A
T7334 1332-1334 , denotes ,
T7335 1334-1335 DT denotes a
T7337 1336-1339 NN denotes DNA
T7336 1340-1348 NN denotes fragment
T7338 1349-1359 VBG denotes consisting
T7339 1360-1362 IN denotes of
T7340 1363-1364 DT denotes a
T7341 1365-1372 NN denotes portion
T7342 1373-1375 IN denotes of
T7343 1376-1380 NN denotes exon
T7344 1381-1383 CD denotes 10
T7345 1384-1388 WDT denotes that
T7346 1389-1391 VBZ denotes is
T7347 1392-1395 RB denotes not
T7348 1396-1403 JJ denotes present
T7349 1404-1406 IN denotes in
T7350 1407-1410 DT denotes the
T7352 1411-1420 NN denotes targeting
T7351 1421-1427 NN denotes vector
T7353 1427-1429 , denotes ,
T7333 1429-1439 VBZ denotes hybridizes
T7354 1440-1442 IN denotes to
T7355 1443-1445 DT denotes an
T7357 1446-1456 JJ denotes endogenous
T7358 1457-1460 CD denotes 3.1
T7360 1460-1461 HYPH denotes -
T7359 1461-1463 NN denotes kb
T7356 1464-1472 NN denotes fragment
T7361 1473-1476 CC denotes and
T7362 1476-1478 , denotes ,
T7363 1478-1482 IN denotes upon
T7365 1483-1493 JJ denotes homologous
T7366 1494-1507 NN denotes recombination
T7367 1507-1509 , denotes ,
T7364 1509-1511 IN denotes to
T7368 1512-1513 DT denotes a
T7370 1514-1518 CD denotes 13.2
T7372 1518-1519 HYPH denotes -
T7371 1519-1521 NN denotes kb
T7369 1522-1530 NN denotes fragment
T7373 1530-1531 . denotes .
T7374 1531-1626 sentence denotes Lane 1 represents a wild-type ES cell line, and Lane 2 and 3 represent targeted ES cell lines.
T7375 1532-1536 NN denotes Lane
T7377 1537-1538 CD denotes 1
T7376 1539-1549 VBZ denotes represents
T7378 1550-1551 DT denotes a
T7380 1552-1556 JJ denotes wild
T7382 1556-1557 HYPH denotes -
T7381 1557-1561 NN denotes type
T7383 1562-1564 NN denotes ES
T7384 1565-1569 NN denotes cell
T7379 1570-1574 NN denotes line
T7385 1574-1576 , denotes ,
T7386 1576-1579 CC denotes and
T7387 1580-1584 NN denotes Lane
T7388 1585-1586 CD denotes 2
T7390 1587-1590 CC denotes and
T7391 1591-1592 CD denotes 3
T7389 1593-1602 VBP denotes represent
T7392 1603-1611 VBN denotes targeted
T7394 1612-1614 NN denotes ES
T7395 1615-1619 NN denotes cell
T7393 1620-1625 NNS denotes lines
T7396 1625-1626 . denotes .
T1138 1627-1629 NN denotes ES
T1139 1630-1634 NN denotes cell
T1140 1635-1641 NNS denotes clones
T1142 1642-1646 VBD denotes were
T1141 1647-1655 VBN denotes screened
T1143 1656-1658 IN denotes by
T1144 1659-1662 NN denotes PCR
T1145 1663-1664 -LRB- denotes (
T1147 1664-1668 NNS denotes data
T1148 1669-1672 RB denotes not
T1146 1673-1678 VBN denotes shown
T1149 1678-1679 -RRB- denotes )
T1150 1680-1683 CC denotes and
T1151 1684-1693 VBN denotes confirmed
T1152 1694-1696 IN denotes by
T1153 1697-1705 NNP denotes Southern
T1154 1706-1710 NN denotes blot
T1155 1711-1719 NN denotes analysis
T1156 1719-1720 . denotes .
T1157 1720-1960 sentence denotes Southern blot analysis used an exon 10 probe (probe A), not present in the targeting vector, hybridized to a 13.2-kb band in addition to the 3.1-kb endogenous band indicating targeted insertion of the vector at the Acadm locus (Figure 1B).
T1158 1721-1729 NNP denotes Southern
T1159 1730-1734 NN denotes blot
T1160 1735-1743 NN denotes analysis
T1161 1744-1748 VBD denotes used
T1162 1749-1751 DT denotes an
T1164 1752-1756 NN denotes exon
T1165 1757-1759 CD denotes 10
T1163 1760-1765 NN denotes probe
T1166 1766-1767 -LRB- denotes (
T1168 1767-1772 NN denotes probe
T1167 1773-1774 NN denotes A
T1169 1774-1775 -RRB- denotes )
T1170 1775-1777 , denotes ,
T1171 1777-1780 RB denotes not
T1172 1781-1788 JJ denotes present
T1173 1789-1791 IN denotes in
T1174 1792-1795 DT denotes the
T1176 1796-1805 NN denotes targeting
T1175 1806-1812 NN denotes vector
T1177 1812-1814 , denotes ,
T1178 1814-1824 VBN denotes hybridized
T1179 1825-1827 IN denotes to
T1180 1828-1829 DT denotes a
T1182 1830-1834 CD denotes 13.2
T1184 1834-1835 HYPH denotes -
T1183 1835-1837 NN denotes kb
T1181 1838-1842 NN denotes band
T1185 1843-1845 IN denotes in
T1186 1846-1854 NN denotes addition
T1187 1855-1857 IN denotes to
T1188 1858-1861 DT denotes the
T1190 1862-1865 CD denotes 3.1
T1192 1865-1866 HYPH denotes -
T1191 1866-1868 NN denotes kb
T1193 1869-1879 JJ denotes endogenous
T1189 1880-1884 NN denotes band
T1194 1885-1895 VBG denotes indicating
T1195 1896-1904 VBN denotes targeted
T1196 1905-1914 NN denotes insertion
T1197 1915-1917 IN denotes of
T1198 1918-1921 DT denotes the
T1199 1922-1928 NN denotes vector
T1200 1929-1931 IN denotes at
T1201 1932-1935 DT denotes the
T1203 1936-1941 NN denotes Acadm
T1202 1942-1947 NN denotes locus
T1204 1948-1949 -LRB- denotes (
T1205 1949-1955 NN denotes Figure
T1206 1956-1958 CD denotes 1B
T1207 1958-1959 -RRB- denotes )
T1208 1959-1960 . denotes .
T1209 1960-2074 sentence denotes Correctly targeted ES cell clones were microinjected into B6 (C57BL/6NTac) blastocysts to generate chimeric mice.
T1210 1961-1970 RB denotes Correctly
T1211 1971-1979 VBN denotes targeted
T1213 1980-1982 NN denotes ES
T1214 1983-1987 NN denotes cell
T1212 1988-1994 NNS denotes clones
T1216 1995-1999 VBD denotes were
T1215 2000-2013 VBN denotes microinjected
T1217 2014-2018 IN denotes into
T1218 2019-2021 NN denotes B6
T1220 2022-2023 -LRB- denotes (
T1221 2023-2028 NN denotes C57BL
T1223 2028-2029 HYPH denotes /
T1222 2029-2034 NN denotes 6NTac
T1224 2034-2035 -RRB- denotes )
T1219 2036-2047 NNS denotes blastocysts
T1225 2048-2050 TO denotes to
T1226 2051-2059 VB denotes generate
T1227 2060-2068 JJ denotes chimeric
T1228 2069-2073 NNS denotes mice
T1229 2073-2074 . denotes .
T1230 2074-2215 sentence denotes Chimeric mice were backcrossed to both 129P2 and B6 inbred mice to produce MCAD+/− and eventually MCAD−/− mice on a B6/129 mixed background.
T1231 2075-2083 JJ denotes Chimeric
T1232 2084-2088 NNS denotes mice
T1234 2089-2093 VBD denotes were
T1233 2094-2105 VBN denotes backcrossed
T1235 2106-2108 IN denotes to
T1236 2109-2113 CC denotes both
T1237 2114-2119 NN denotes 129P2
T1239 2120-2123 CC denotes and
T1240 2124-2126 NN denotes B6
T1238 2127-2133 VBN denotes inbred
T1241 2134-2138 NNS denotes mice
T1242 2139-2141 TO denotes to
T1243 2142-2149 VB denotes produce
T1244 2150-2154 NN denotes MCAD
T1246 2154-2155 SYM denotes +
T1247 2155-2156 HYPH denotes /
T1248 2156-2157 SYM denotes −
T1249 2158-2161 CC denotes and
T1250 2162-2172 RB denotes eventually
T1251 2173-2177 NN denotes MCAD
T1252 2177-2178 SYM denotes −
T1253 2178-2179 HYPH denotes /
T1254 2179-2180 SYM denotes −
T1245 2181-2185 NNS denotes mice
T1255 2186-2188 IN denotes on
T1256 2189-2190 DT denotes a
T1258 2191-2193 NN denotes B6
T1259 2193-2194 HYPH denotes /
T1260 2194-2197 CD denotes 129
T1261 2198-2203 JJ denotes mixed
T1257 2204-2214 NN denotes background
T1262 2214-2215 . denotes .
T1263 2215-2407 sentence denotes The studies described here were conducted exclusively on the B6/129 mixed background compared with littermate controls or B6/129 control groups maintained by intercrosses as were the mutants.
T1264 2216-2219 DT denotes The
T1265 2220-2227 NNS denotes studies
T1267 2228-2237 VBN denotes described
T1268 2238-2242 RB denotes here
T1269 2243-2247 VBD denotes were
T1266 2248-2257 VBN denotes conducted
T1270 2258-2269 RB denotes exclusively
T1271 2270-2272 IN denotes on
T1272 2273-2276 DT denotes the
T1274 2277-2279 NN denotes B6
T1275 2279-2280 HYPH denotes /
T1276 2280-2283 CD denotes 129
T1277 2284-2289 JJ denotes mixed
T1273 2290-2300 NN denotes background
T1278 2301-2309 VBN denotes compared
T1279 2310-2314 IN denotes with
T1280 2315-2325 NN denotes littermate
T1281 2326-2334 NNS denotes controls
T1282 2335-2337 CC denotes or
T1283 2338-2340 NN denotes B6
T1285 2340-2341 HYPH denotes /
T1286 2341-2344 CD denotes 129
T1287 2345-2352 NN denotes control
T1284 2353-2359 NNS denotes groups
T1288 2360-2370 VBN denotes maintained
T1289 2371-2373 IN denotes by
T1290 2374-2386 NNS denotes intercrosses
T1291 2387-2389 IN denotes as
T1292 2390-2394 VBD denotes were
T1293 2395-2398 DT denotes the
T1294 2399-2406 NNS denotes mutants
T1295 2406-2407 . denotes .
T1296 2407-2504 sentence denotes Perpetuating this mutation as a congenic mutant line on the 129P2 background proved impractical.
T1297 2408-2420 VBG denotes Perpetuating
T1299 2421-2425 DT denotes this
T1300 2426-2434 NN denotes mutation
T1301 2435-2437 IN denotes as
T1302 2438-2439 DT denotes a
T1304 2440-2448 JJ denotes congenic
T1305 2449-2455 NN denotes mutant
T1303 2456-2460 NN denotes line
T1306 2461-2463 IN denotes on
T1307 2464-2467 DT denotes the
T1309 2468-2473 NN denotes 129P2
T1308 2474-2484 NN denotes background
T1298 2485-2491 VBD denotes proved
T1310 2492-2503 JJ denotes impractical
T1311 2503-2504 . denotes .
T1312 2504-2672 sentence denotes The 129P2 mice were poor breeders as wild-types, and when introduced, the Acadm mutation was nearly lost on this background because of the high rate of neonatal death.
T1313 2505-2508 DT denotes The
T1315 2509-2514 NN denotes 129P2
T1314 2515-2519 NNS denotes mice
T1316 2520-2524 VBD denotes were
T1317 2525-2529 JJ denotes poor
T1318 2530-2538 NNS denotes breeders
T1319 2539-2541 IN denotes as
T1320 2542-2546 JJ denotes wild
T1322 2546-2547 HYPH denotes -
T1321 2547-2552 NNS denotes types
T1323 2552-2554 , denotes ,
T1324 2554-2557 CC denotes and
T1325 2558-2562 WRB denotes when
T1326 2563-2573 VBN denotes introduced
T1328 2573-2575 , denotes ,
T1329 2575-2578 DT denotes the
T1331 2579-2584 NN denotes Acadm
T1330 2585-2593 NN denotes mutation
T1332 2594-2597 VBD denotes was
T1333 2598-2604 RB denotes nearly
T1327 2605-2609 VBN denotes lost
T1334 2610-2612 IN denotes on
T1335 2613-2617 DT denotes this
T1336 2618-2628 NN denotes background
T1337 2629-2636 IN denotes because
T1338 2637-2639 IN denotes of
T1339 2640-2643 DT denotes the
T1341 2644-2648 JJ denotes high
T1340 2649-2653 NN denotes rate
T1342 2654-2656 IN denotes of
T1343 2657-2665 JJ denotes neonatal
T1344 2666-2671 NN denotes death
T1345 2671-2672 . denotes .
T1346 2672-2808 sentence denotes Because of the molecular structure of the targeted allele, it proved virtually impossible to distinguish all three potential genotypes.
T1347 2673-2680 IN denotes Because
T1349 2681-2683 IN denotes of
T1350 2684-2687 DT denotes the
T1352 2688-2697 JJ denotes molecular
T1351 2698-2707 NN denotes structure
T1353 2708-2710 IN denotes of
T1354 2711-2714 DT denotes the
T1356 2715-2723 VBN denotes targeted
T1355 2724-2730 NN denotes allele
T1357 2730-2732 , denotes ,
T1358 2732-2734 PRP denotes it
T1348 2735-2741 VBD denotes proved
T1359 2742-2751 RB denotes virtually
T1360 2752-2762 JJ denotes impossible
T1361 2763-2765 TO denotes to
T1362 2766-2777 VB denotes distinguish
T1363 2778-2781 DT denotes all
T1365 2782-2787 CD denotes three
T1366 2788-2797 JJ denotes potential
T1364 2798-2807 NNS denotes genotypes
T1367 2807-2808 . denotes .
T1368 2808-3003 sentence denotes We could clearly detect the presence or absence of the targeted allele, however, whether a particular mouse was MCAD−/− or MCAD+/− could not be determined by Southern blot or PCR of genomic DNA.
T1369 2809-2811 PRP denotes We
T1371 2812-2817 MD denotes could
T1372 2818-2825 RB denotes clearly
T1370 2826-2832 VB denotes detect
T1374 2833-2836 DT denotes the
T1375 2837-2845 NN denotes presence
T1376 2846-2848 CC denotes or
T1377 2849-2856 NN denotes absence
T1378 2857-2859 IN denotes of
T1379 2860-2863 DT denotes the
T1381 2864-2872 VBN denotes targeted
T1380 2873-2879 NN denotes allele
T1382 2879-2881 , denotes ,
T1383 2881-2888 RB denotes however
T1384 2888-2890 , denotes ,
T1385 2890-2897 IN denotes whether
T1387 2898-2899 DT denotes a
T1389 2900-2910 JJ denotes particular
T1388 2911-2916 NN denotes mouse
T1386 2917-2920 VBD denotes was
T1390 2921-2925 NN denotes MCAD
T1391 2925-2926 SYM denotes −
T1392 2926-2927 HYPH denotes /
T1393 2927-2928 SYM denotes −
T1394 2929-2931 CC denotes or
T1395 2932-2936 NN denotes MCAD
T1396 2936-2937 SYM denotes +
T1397 2937-2938 HYPH denotes /
T1398 2938-2939 SYM denotes −
T1399 2940-2945 MD denotes could
T1400 2946-2949 RB denotes not
T1401 2950-2952 VB denotes be
T1373 2953-2963 VBN denotes determined
T1402 2964-2966 IN denotes by
T1403 2967-2975 NNP denotes Southern
T1404 2976-2980 NN denotes blot
T1405 2981-2983 CC denotes or
T1406 2984-2987 NN denotes PCR
T1407 2988-2990 IN denotes of
T1408 2991-2998 JJ denotes genomic
T1409 2999-3002 NN denotes DNA
T1410 3002-3003 . denotes .
T1411 3003-3157 sentence denotes Ultimately MCAD−/− mice were ascertained by immunoblot analysis of offspring with subsequent perpetuation of MCAD−/− and MCAD+/+ mice as separate groups.
T1412 3004-3014 RB denotes Ultimately
T1414 3015-3019 NN denotes MCAD
T1416 3019-3020 SYM denotes −
T1417 3020-3021 HYPH denotes /
T1418 3021-3022 SYM denotes −
T1415 3023-3027 NNS denotes mice
T1419 3028-3032 VBD denotes were
T1413 3033-3044 VBN denotes ascertained
T1420 3045-3047 IN denotes by
T1421 3048-3058 NN denotes immunoblot
T1422 3059-3067 NN denotes analysis
T1423 3068-3070 IN denotes of
T1424 3071-3080 NN denotes offspring
T1425 3081-3085 IN denotes with
T1426 3086-3096 JJ denotes subsequent
T1427 3097-3109 NN denotes perpetuation
T1428 3110-3112 IN denotes of
T1429 3113-3117 NN denotes MCAD
T1431 3117-3118 SYM denotes −
T1432 3118-3119 HYPH denotes /
T1433 3119-3120 SYM denotes −
T1434 3121-3124 CC denotes and
T1435 3125-3129 NN denotes MCAD
T1436 3129-3130 SYM denotes +
T1437 3130-3131 HYPH denotes /
T1438 3131-3132 SYM denotes +
T1430 3133-3137 NNS denotes mice
T1439 3138-3140 IN denotes as
T1440 3141-3149 JJ denotes separate
T1441 3150-3156 NNS denotes groups
T1442 3156-3157 . denotes .
T1606 3159-3162 NN denotes RNA
T1607 3163-3171 NN denotes Analysis
T1608 3171-3367 sentence denotes RT-PCR amplification from exon 7 to 11 from total heart RNA amplified the expected 600-base pair (bp) fragment in MCAD+/+ and MCAD+/− mice, and a 1.5-kb fragment in MCAD−/− mice (data not shown).
T1609 3172-3174 NN denotes RT
T1611 3174-3175 HYPH denotes -
T1610 3175-3178 NN denotes PCR
T1612 3179-3192 NN denotes amplification
T1614 3193-3197 IN denotes from
T1615 3198-3202 NN denotes exon
T1616 3203-3204 CD denotes 7
T1617 3205-3207 IN denotes to
T1618 3208-3210 CD denotes 11
T1619 3211-3215 IN denotes from
T1620 3216-3221 JJ denotes total
T1622 3222-3227 NN denotes heart
T1621 3228-3231 NN denotes RNA
T1613 3232-3241 VBD denotes amplified
T1623 3242-3245 DT denotes the
T1625 3246-3254 VBN denotes expected
T1626 3255-3258 CD denotes 600
T1628 3258-3259 HYPH denotes -
T1629 3259-3263 NN denotes base
T1627 3264-3268 NN denotes pair
T1630 3269-3270 -LRB- denotes (
T1631 3270-3272 NN denotes bp
T1632 3272-3273 -RRB- denotes )
T1624 3274-3282 NN denotes fragment
T1633 3283-3285 IN denotes in
T1634 3286-3290 NN denotes MCAD
T1636 3290-3291 SYM denotes +
T1637 3291-3292 HYPH denotes /
T1638 3292-3293 SYM denotes +
T1639 3294-3297 CC denotes and
T1640 3298-3302 NN denotes MCAD
T1641 3302-3303 SYM denotes +
T1642 3303-3304 HYPH denotes /
T1643 3304-3305 SYM denotes −
T1635 3306-3310 NNS denotes mice
T1644 3310-3312 , denotes ,
T1645 3312-3315 CC denotes and
T1646 3316-3317 DT denotes a
T1648 3318-3321 CD denotes 1.5
T1650 3321-3322 HYPH denotes -
T1649 3322-3324 NN denotes kb
T1647 3325-3333 NN denotes fragment
T1651 3334-3336 IN denotes in
T1652 3337-3341 NN denotes MCAD
T1654 3341-3342 SYM denotes −
T1655 3342-3343 HYPH denotes /
T1656 3343-3344 SYM denotes −
T1653 3345-3349 NNS denotes mice
T1657 3350-3351 -LRB- denotes (
T1659 3351-3355 NNS denotes data
T1660 3356-3359 RB denotes not
T1658 3360-3365 VBN denotes shown
T1661 3365-3366 -RRB- denotes )
T1662 3366-3367 . denotes .
T1663 3367-3537 sentence denotes Sequence analysis of the 1.5-kb fragment revealed that the amplified fragment consisted of exon 7 to exon 10 with 280 bp of pGEM plasmid sequence followed by exons 8–11.
T1664 3368-3376 NN denotes Sequence
T1665 3377-3385 NN denotes analysis
T1667 3386-3388 IN denotes of
T1668 3389-3392 DT denotes the
T1670 3393-3396 CD denotes 1.5
T1672 3396-3397 HYPH denotes -
T1671 3397-3399 NN denotes kb
T1669 3400-3408 NN denotes fragment
T1666 3409-3417 VBD denotes revealed
T1673 3418-3422 IN denotes that
T1675 3423-3426 DT denotes the
T1677 3427-3436 VBN denotes amplified
T1676 3437-3445 NN denotes fragment
T1674 3446-3455 VBD denotes consisted
T1678 3456-3458 IN denotes of
T1679 3459-3463 NN denotes exon
T1680 3464-3465 CD denotes 7
T1681 3466-3468 IN denotes to
T1682 3469-3473 NN denotes exon
T1683 3474-3476 CD denotes 10
T1684 3477-3481 IN denotes with
T1685 3482-3485 CD denotes 280
T1686 3486-3488 NN denotes bp
T1687 3489-3491 IN denotes of
T1688 3492-3496 NN denotes pGEM
T1690 3497-3504 NN denotes plasmid
T1689 3505-3513 NN denotes sequence
T1691 3514-3522 VBN denotes followed
T1692 3523-3525 IN denotes by
T1693 3526-3531 NNS denotes exons
T1694 3532-3533 CD denotes 8
T1695 3533-3534 SYM denotes –
T1696 3534-3536 CD denotes 11
T1697 3536-3537 . denotes .
T1698 3537-3640 sentence denotes Some of the plasmid sequences, along with the pPGKNEOpA sequence, were deleted from this spliced mRNA.
T1699 3538-3542 DT denotes Some
T1701 3543-3545 IN denotes of
T1702 3546-3549 DT denotes the
T1704 3550-3557 NN denotes plasmid
T1703 3558-3567 NNS denotes sequences
T1705 3567-3569 , denotes ,
T1706 3569-3574 IN denotes along
T1707 3575-3579 IN denotes with
T1708 3580-3583 DT denotes the
T1710 3584-3593 NN denotes pPGKNEOpA
T1709 3594-3602 NN denotes sequence
T1711 3602-3604 , denotes ,
T1712 3604-3608 VBD denotes were
T1700 3609-3616 VBN denotes deleted
T1713 3617-3621 IN denotes from
T1714 3622-3626 DT denotes this
T1716 3627-3634 VBN denotes spliced
T1715 3635-3639 NN denotes mRNA
T1717 3639-3640 . denotes .
T1718 3640-3913 sentence denotes Northern blot analysis revealed Acadm was normally expressed in all tissues analyzed from MCAD+/+ mice with the most robust expression occurring in brown fat, kidney, heart, skeletal muscle, and liver with minimal expression in the brain, white fat, and testes (Figure 2).
T1719 3641-3649 NNP denotes Northern
T1720 3650-3654 NN denotes blot
T1721 3655-3663 NN denotes analysis
T1722 3664-3672 VBD denotes revealed
T1723 3673-3678 NN denotes Acadm
T1725 3679-3682 VBD denotes was
T1726 3683-3691 RB denotes normally
T1724 3692-3701 VBN denotes expressed
T1727 3702-3704 IN denotes in
T1728 3705-3708 DT denotes all
T1729 3709-3716 NNS denotes tissues
T1730 3717-3725 VBN denotes analyzed
T1731 3726-3730 IN denotes from
T1732 3731-3735 NN denotes MCAD
T1734 3735-3736 SYM denotes +
T1735 3736-3737 HYPH denotes /
T1736 3737-3738 SYM denotes +
T1733 3739-3743 NNS denotes mice
T1737 3744-3748 IN denotes with
T1738 3749-3752 DT denotes the
T1740 3753-3757 RBS denotes most
T1741 3758-3764 JJ denotes robust
T1739 3765-3775 NN denotes expression
T1742 3776-3785 VBG denotes occurring
T1743 3786-3788 IN denotes in
T1744 3789-3794 JJ denotes brown
T1745 3795-3798 NN denotes fat
T1746 3798-3800 , denotes ,
T1747 3800-3806 NN denotes kidney
T1748 3806-3808 , denotes ,
T1749 3808-3813 NN denotes heart
T1750 3813-3815 , denotes ,
T1751 3815-3823 JJ denotes skeletal
T1752 3824-3830 NN denotes muscle
T1753 3830-3832 , denotes ,
T1754 3832-3835 CC denotes and
T1755 3836-3841 NN denotes liver
T1756 3842-3846 IN denotes with
T1757 3847-3854 JJ denotes minimal
T1758 3855-3865 NN denotes expression
T1759 3866-3868 IN denotes in
T1760 3869-3872 DT denotes the
T1761 3873-3878 NN denotes brain
T1762 3878-3880 , denotes ,
T1763 3880-3885 JJ denotes white
T1764 3886-3889 NN denotes fat
T1765 3889-3891 , denotes ,
T1766 3891-3894 CC denotes and
T1767 3895-3901 NNS denotes testes
T1768 3902-3903 -LRB- denotes (
T1769 3903-3909 NN denotes Figure
T1770 3910-3911 CD denotes 2
T1771 3911-3912 -RRB- denotes )
T1772 3912-3913 . denotes .
T1773 3913-4075 sentence denotes Interestingly, although RT-PCR amplified an incorrectly spliced Acadm transcript, no Acadm transcripts were detected by northern blot analysis from MCAD−/− mice.
T1774 3914-3927 RB denotes Interestingly
T1776 3927-3929 , denotes ,
T1777 3929-3937 IN denotes although
T1779 3938-3940 NN denotes RT
T1781 3940-3941 HYPH denotes -
T1780 3941-3944 NN denotes PCR
T1778 3945-3954 VBD denotes amplified
T1782 3955-3957 DT denotes an
T1784 3958-3969 RB denotes incorrectly
T1785 3970-3977 VBN denotes spliced
T1786 3978-3983 NN denotes Acadm
T1783 3984-3994 NN denotes transcript
T1787 3994-3996 , denotes ,
T1788 3996-3998 DT denotes no
T1790 3999-4004 NN denotes Acadm
T1789 4005-4016 NNS denotes transcripts
T1791 4017-4021 VBD denotes were
T1775 4022-4030 VBN denotes detected
T1792 4031-4033 IN denotes by
T1793 4034-4042 NNP denotes northern
T1794 4043-4047 NN denotes blot
T1795 4048-4056 NN denotes analysis
T1796 4057-4061 IN denotes from
T1797 4062-4066 NN denotes MCAD
T1799 4066-4067 SYM denotes −
T1800 4067-4068 HYPH denotes /
T1801 4068-4069 SYM denotes −
T1798 4070-4074 NNS denotes mice
T1802 4074-4075 . denotes .
T1803 4075-4217 sentence denotes These results strongly suggest that the mutant RNA is unstable and degraded rapidly or, alternatively, undergoes nonsense mediated RNA decay.
T1804 4076-4081 DT denotes These
T1805 4082-4089 NNS denotes results
T1807 4090-4098 RB denotes strongly
T1806 4099-4106 VBP denotes suggest
T1808 4107-4111 IN denotes that
T1810 4112-4115 DT denotes the
T1812 4116-4122 NN denotes mutant
T1811 4123-4126 NN denotes RNA
T1809 4127-4129 VBZ denotes is
T1813 4130-4138 JJ denotes unstable
T1814 4139-4142 CC denotes and
T1815 4143-4151 VBN denotes degraded
T1816 4152-4159 RB denotes rapidly
T1817 4160-4162 CC denotes or
T1818 4162-4164 , denotes ,
T1819 4164-4177 RB denotes alternatively
T1821 4177-4179 , denotes ,
T1820 4179-4188 VBZ denotes undergoes
T1822 4189-4197 NN denotes nonsense
T1823 4198-4206 VBN denotes mediated
T1825 4207-4210 NN denotes RNA
T1824 4211-4216 NN denotes decay
T1826 4216-4217 . denotes .
T7455 4228-4236 NNP denotes Northern
T7456 4237-4241 NN denotes Blot
T7457 4242-4250 NN denotes Analysis
T7458 4251-4255 IN denotes from
T7459 4256-4260 NN denotes MCAD
T7461 4260-4261 SYM denotes −
T7462 4261-4262 HYPH denotes /
T7463 4262-4263 SYM denotes −
T7464 4264-4265 -LRB- denotes (
T7466 4265-4266 NN denotes n
T7467 4267-4268 SYM denotes =
T7465 4269-4270 CD denotes 2
T7468 4270-4271 -RRB- denotes )
T7469 4272-4275 CC denotes and
T7470 4276-4280 NN denotes MCAD
T7471 4280-4281 SYM denotes +
T7472 4281-4282 HYPH denotes /
T7473 4282-4283 SYM denotes +
T7474 4284-4285 -LRB- denotes (
T7476 4285-4286 NN denotes n
T7477 4287-4288 SYM denotes =
T7475 4289-4290 CD denotes 2
T7478 4290-4291 -RRB- denotes )
T7460 4292-4296 NNS denotes Mice
T7479 4296-4448 sentence denotes Acadm message was detected from the heart, liver, brown fat, brain, kidney, and muscle (and white fat and testes, data not shown) of only MCAD+/+ mice.
T7480 4297-4302 NN denotes Acadm
T7481 4303-4310 NN denotes message
T7483 4311-4314 VBD denotes was
T7482 4315-4323 VBN denotes detected
T7484 4324-4328 IN denotes from
T7485 4329-4332 DT denotes the
T7486 4333-4338 NN denotes heart
T7487 4338-4340 , denotes ,
T7488 4340-4345 NN denotes liver
T7489 4345-4347 , denotes ,
T7490 4347-4352 JJ denotes brown
T7491 4353-4356 NN denotes fat
T7492 4356-4358 , denotes ,
T7493 4358-4363 NN denotes brain
T7494 4363-4365 , denotes ,
T7495 4365-4371 NN denotes kidney
T7496 4371-4373 , denotes ,
T7497 4373-4376 CC denotes and
T7498 4377-4383 NN denotes muscle
T7499 4384-4385 -LRB- denotes (
T7500 4385-4388 CC denotes and
T7501 4389-4394 JJ denotes white
T7502 4395-4398 NN denotes fat
T7503 4399-4402 CC denotes and
T7504 4403-4409 NNS denotes testes
T7505 4409-4411 , denotes ,
T7507 4411-4415 NNS denotes data
T7508 4416-4419 RB denotes not
T7506 4420-4425 VBN denotes shown
T7509 4425-4426 -RRB- denotes )
T7510 4427-4429 IN denotes of
T7511 4430-4434 JJ denotes only
T7513 4435-4439 NN denotes MCAD
T7514 4439-4440 SYM denotes +
T7515 4440-4441 HYPH denotes /
T7516 4441-4442 SYM denotes +
T7512 4443-4447 NNS denotes mice
T7517 4447-4448 . denotes .
T7518 4448-4530 sentence denotes Most robust expression occurred in brown fat, kidney, heart, and skeletal muscle.
T7519 4449-4453 RBS denotes Most
T7521 4454-4460 JJ denotes robust
T7520 4461-4471 NN denotes expression
T7522 4472-4480 VBD denotes occurred
T7523 4481-4483 IN denotes in
T7524 4484-4489 JJ denotes brown
T7525 4490-4493 NN denotes fat
T7526 4493-4495 , denotes ,
T7527 4495-4501 NN denotes kidney
T7528 4501-4503 , denotes ,
T7529 4503-4508 NN denotes heart
T7530 4508-4510 , denotes ,
T7531 4510-4513 CC denotes and
T7532 4514-4522 JJ denotes skeletal
T7533 4523-4529 NN denotes muscle
T7534 4529-4530 . denotes .
T7535 4530-4594 sentence denotes MCAD−/− mice had no detectable message in all tissues examined.
T7536 4531-4535 NN denotes MCAD
T7538 4535-4536 SYM denotes −
T7539 4536-4537 HYPH denotes /
T7540 4537-4538 SYM denotes −
T7537 4539-4543 NNS denotes mice
T7541 4544-4547 VBD denotes had
T7542 4548-4550 DT denotes no
T7544 4551-4561 JJ denotes detectable
T7543 4562-4569 NN denotes message
T7545 4570-4572 IN denotes in
T7546 4573-4576 DT denotes all
T7547 4577-4584 NNS denotes tissues
T7548 4585-4593 VBN denotes examined
T7549 4593-4594 . denotes .
T1979 4596-4601 NN denotes Liver
T1980 4602-4608 NN denotes Enzyme
T1981 4609-4617 NNS denotes Analyses
T1982 4617-4789 sentence denotes Immunoblot analyses of liver homogenates with anti-MCAD antisera demonstrated that the 42 kDa MCAD monomer was present in MCAD+/+ mice, but not in MCAD−/− mice (Figure 3).
T1983 4618-4628 NN denotes Immunoblot
T1984 4629-4637 NNS denotes analyses
T1986 4638-4640 IN denotes of
T1987 4641-4646 NN denotes liver
T1988 4647-4658 NNS denotes homogenates
T1989 4659-4663 IN denotes with
T1990 4664-4673 JJ denotes anti-MCAD
T1991 4674-4682 NNS denotes antisera
T1985 4683-4695 VBD denotes demonstrated
T1992 4696-4700 IN denotes that
T1994 4701-4704 DT denotes the
T1996 4705-4707 CD denotes 42
T1997 4708-4711 NN denotes kDa
T1998 4712-4716 NN denotes MCAD
T1995 4717-4724 NN denotes monomer
T1993 4725-4728 VBD denotes was
T1999 4729-4736 JJ denotes present
T2000 4737-4739 IN denotes in
T2001 4740-4744 NN denotes MCAD
T2003 4744-4745 SYM denotes +
T2004 4745-4746 HYPH denotes /
T2005 4746-4747 SYM denotes +
T2002 4748-4752 NNS denotes mice
T2006 4752-4754 , denotes ,
T2007 4754-4757 CC denotes but
T2008 4758-4761 RB denotes not
T2009 4762-4764 IN denotes in
T2010 4765-4769 NN denotes MCAD
T2012 4769-4770 SYM denotes −
T2013 4770-4771 HYPH denotes /
T2014 4771-4772 SYM denotes −
T2011 4773-4777 NNS denotes mice
T2015 4778-4779 -LRB- denotes (
T2016 4779-4785 NN denotes Figure
T2017 4786-4787 CD denotes 3
T2018 4787-4788 -RRB- denotes )
T2019 4788-4789 . denotes .
T2020 4789-4977 sentence denotes As a control analysis, anti–short-chain acyl-CoA dehydrogenase (SCAD) antisera revealed no differences in levels of expression of SCAD protein between MCAD+/+ and MCAD−/− mice (Figure 3).
T2021 4790-4792 IN denotes As
T2023 4793-4794 DT denotes a
T2025 4795-4802 NN denotes control
T2024 4803-4811 NN denotes analysis
T2026 4811-4813 , denotes ,
T2027 4813-4823 JJ denotes anti–short
T2029 4823-4824 HYPH denotes -
T2028 4824-4829 NN denotes chain
T2031 4830-4834 NN denotes acyl
T2033 4834-4835 HYPH denotes -
T2032 4835-4838 NN denotes CoA
T2030 4839-4852 NN denotes dehydrogenase
T2035 4853-4854 -LRB- denotes (
T2034 4854-4858 NN denotes SCAD
T2037 4858-4859 -RRB- denotes )
T2036 4860-4868 NNS denotes antisera
T2022 4869-4877 VBD denotes revealed
T2038 4878-4880 DT denotes no
T2039 4881-4892 NNS denotes differences
T2040 4893-4895 IN denotes in
T2041 4896-4902 NNS denotes levels
T2042 4903-4905 IN denotes of
T2043 4906-4916 NN denotes expression
T2044 4917-4919 IN denotes of
T2045 4920-4924 NN denotes SCAD
T2046 4925-4932 NN denotes protein
T2047 4933-4940 IN denotes between
T2048 4941-4945 NN denotes MCAD
T2050 4945-4946 SYM denotes +
T2051 4946-4947 HYPH denotes /
T2052 4947-4948 SYM denotes +
T2053 4949-4952 CC denotes and
T2054 4953-4957 NN denotes MCAD
T2055 4957-4958 SYM denotes −
T2056 4958-4959 HYPH denotes /
T2057 4959-4960 SYM denotes −
T2049 4961-4965 NNS denotes mice
T2058 4966-4967 -LRB- denotes (
T2059 4967-4973 NN denotes Figure
T2060 4974-4975 CD denotes 3
T2061 4975-4976 -RRB- denotes )
T2062 4976-4977 . denotes .
T2063 4977-5703 sentence denotes Figure 3 Immunoblots of Liver Homogenates from MCAD+/+ and MCAD−/− Mice These were probed with anti-MCAD antibody or anti-SCAD antibody. Homozygous MCAD−/− mice had no detectable MCAD protein. MCAD protein is only detectable under the MCAD protein–spiked (positive control) lane. As a control analysis, liver homogenates probed with anti-SCAD antibody revealed that SCAD protein was present in both MCAD+/+ and MCAD−/− mice. No MCAD positive-control protein is detected by anti-SCAD antibodies (MCAD lane). mw, molecular weight standards. Enzyme activity was analyzed in mouse liver homogenates using the electron transport flavoprotein (ETF) reduction assay with octanoyl-CoA (C8:0) and palmitoyl-CoA (C16:0) as substrates.
T7638 4988-4999 NNS denotes Immunoblots
T7639 5000-5002 IN denotes of
T7640 5003-5008 NN denotes Liver
T7641 5009-5020 NNS denotes Homogenates
T7642 5021-5025 IN denotes from
T7643 5026-5030 NN denotes MCAD
T7645 5030-5031 SYM denotes +
T7646 5031-5032 HYPH denotes /
T7647 5032-5033 SYM denotes +
T7648 5034-5037 CC denotes and
T7649 5038-5042 NN denotes MCAD
T7650 5042-5043 SYM denotes −
T7651 5043-5044 HYPH denotes /
T7652 5044-5045 SYM denotes −
T7644 5046-5050 NNS denotes Mice
T7653 5050-5115 sentence denotes These were probed with anti-MCAD antibody or anti-SCAD antibody.
T7654 5051-5056 DT denotes These
T7656 5057-5061 VBD denotes were
T7655 5062-5068 VBN denotes probed
T7657 5069-5073 IN denotes with
T7658 5074-5083 JJ denotes anti-MCAD
T7659 5084-5092 NN denotes antibody
T7660 5093-5095 CC denotes or
T7661 5096-5105 JJ denotes anti-SCAD
T7662 5106-5114 NN denotes antibody
T7663 5114-5115 . denotes .
T7664 5115-5171 sentence denotes Homozygous MCAD−/− mice had no detectable MCAD protein.
T7665 5116-5126 JJ denotes Homozygous
T7667 5127-5131 NN denotes MCAD
T7668 5131-5132 SYM denotes −
T7669 5132-5133 HYPH denotes /
T7670 5133-5134 SYM denotes −
T7666 5135-5139 NNS denotes mice
T7671 5140-5143 VBD denotes had
T7672 5144-5146 DT denotes no
T7674 5147-5157 JJ denotes detectable
T7675 5158-5162 NN denotes MCAD
T7673 5163-5170 NN denotes protein
T7676 5170-5171 . denotes .
T7677 5171-5258 sentence denotes MCAD protein is only detectable under the MCAD protein–spiked (positive control) lane.
T7678 5172-5176 NN denotes MCAD
T7679 5177-5184 NN denotes protein
T7680 5185-5187 VBZ denotes is
T7681 5188-5192 RB denotes only
T7682 5193-5203 JJ denotes detectable
T7683 5204-5209 IN denotes under
T7684 5210-5213 DT denotes the
T7686 5214-5218 NN denotes MCAD
T7687 5219-5226 NN denotes protein
T7689 5226-5227 HYPH denotes –
T7688 5227-5233 VBN denotes spiked
T7690 5234-5235 -LRB- denotes (
T7692 5235-5243 JJ denotes positive
T7691 5244-5251 NN denotes control
T7693 5251-5252 -RRB- denotes )
T7685 5253-5257 NN denotes lane
T7694 5257-5258 . denotes .
T7695 5258-5403 sentence denotes As a control analysis, liver homogenates probed with anti-SCAD antibody revealed that SCAD protein was present in both MCAD+/+ and MCAD−/− mice.
T7696 5259-5261 IN denotes As
T7698 5262-5263 DT denotes a
T7700 5264-5271 NN denotes control
T7699 5272-5280 NN denotes analysis
T7701 5280-5282 , denotes ,
T7702 5282-5287 NN denotes liver
T7703 5288-5299 NNS denotes homogenates
T7704 5300-5306 VBN denotes probed
T7705 5307-5311 IN denotes with
T7706 5312-5321 JJ denotes anti-SCAD
T7707 5322-5330 NN denotes antibody
T7697 5331-5339 VBD denotes revealed
T7708 5340-5344 IN denotes that
T7710 5345-5349 NN denotes SCAD
T7711 5350-5357 NN denotes protein
T7709 5358-5361 VBD denotes was
T7712 5362-5369 JJ denotes present
T7713 5370-5372 IN denotes in
T7714 5373-5377 CC denotes both
T7715 5378-5382 NN denotes MCAD
T7717 5382-5383 SYM denotes +
T7718 5383-5384 HYPH denotes /
T7719 5384-5385 SYM denotes +
T7720 5386-5389 CC denotes and
T7721 5390-5394 NN denotes MCAD
T7722 5394-5395 SYM denotes −
T7723 5395-5396 HYPH denotes /
T7724 5396-5397 SYM denotes −
T7716 5398-5402 NNS denotes mice
T7725 5402-5403 . denotes .
T7726 5403-5485 sentence denotes No MCAD positive-control protein is detected by anti-SCAD antibodies (MCAD lane).
T7727 5404-5406 DT denotes No
T7729 5407-5411 NN denotes MCAD
T7730 5412-5420 JJ denotes positive
T7732 5420-5421 HYPH denotes -
T7731 5421-5428 NN denotes control
T7728 5429-5436 NN denotes protein
T7734 5437-5439 VBZ denotes is
T7733 5440-5448 VBN denotes detected
T7735 5449-5451 IN denotes by
T7736 5452-5461 JJ denotes anti-SCAD
T7737 5462-5472 NNS denotes antibodies
T7738 5473-5474 -LRB- denotes (
T7740 5474-5478 NN denotes MCAD
T7739 5479-5483 NN denotes lane
T7741 5483-5484 -RRB- denotes )
T7742 5484-5485 . denotes .
T7743 5485-5517 sentence denotes mw, molecular weight standards.
T7744 5486-5488 NN denotes mw
T7745 5488-5490 , denotes ,
T7746 5490-5499 JJ denotes molecular
T7748 5500-5506 NN denotes weight
T7747 5507-5516 NNS denotes standards
T7749 5516-5517 . denotes .
T2064 5518-5524 NN denotes Enzyme
T2065 5525-5533 NN denotes activity
T2067 5534-5537 VBD denotes was
T2066 5538-5546 VBN denotes analyzed
T2068 5547-5549 IN denotes in
T2069 5550-5555 NN denotes mouse
T2071 5556-5561 NN denotes liver
T2070 5562-5573 NNS denotes homogenates
T2072 5574-5579 VBG denotes using
T2073 5580-5583 DT denotes the
T2075 5584-5592 NN denotes electron
T2077 5593-5602 NN denotes transport
T2076 5603-5615 NN denotes flavoprotein
T2078 5616-5617 -LRB- denotes (
T2079 5617-5620 NN denotes ETF
T2080 5620-5621 -RRB- denotes )
T2081 5622-5631 NN denotes reduction
T2074 5632-5637 NN denotes assay
T2082 5638-5642 IN denotes with
T2083 5643-5651 NN denotes octanoyl
T2085 5651-5652 HYPH denotes -
T2084 5652-5655 NN denotes CoA
T2086 5656-5657 -LRB- denotes (
T2087 5657-5659 NN denotes C8
T2088 5659-5660 : denotes :
T2089 5660-5661 CD denotes 0
T2090 5661-5662 -RRB- denotes )
T2091 5663-5666 CC denotes and
T2092 5667-5676 NN denotes palmitoyl
T2094 5676-5677 HYPH denotes -
T2093 5677-5680 NN denotes CoA
T2095 5681-5682 -LRB- denotes (
T2096 5682-5685 NN denotes C16
T2097 5685-5686 : denotes :
T2098 5686-5687 CD denotes 0
T2099 5687-5688 -RRB- denotes )
T2100 5689-5691 IN denotes as
T2101 5692-5702 NNS denotes substrates
T2102 5702-5703 . denotes .
T2103 5703-5877 sentence denotes MCAD−/− mice had a significant reduction in ability to dehydrogenate octanoyl-CoA and a modest reduction in activity toward palmitoyl-CoA compared to MCAD+/+ mice (Table 1).
T2104 5704-5708 NN denotes MCAD
T2106 5708-5709 SYM denotes −
T2107 5709-5710 HYPH denotes /
T2108 5710-5711 SYM denotes −
T2105 5712-5716 NNS denotes mice
T2109 5717-5720 VBD denotes had
T2110 5721-5722 DT denotes a
T2112 5723-5734 JJ denotes significant
T2111 5735-5744 NN denotes reduction
T2113 5745-5747 IN denotes in
T2114 5748-5755 NN denotes ability
T2115 5756-5758 TO denotes to
T2116 5759-5772 VB denotes dehydrogenate
T2117 5773-5781 NN denotes octanoyl
T2119 5781-5782 HYPH denotes -
T2118 5782-5785 NN denotes CoA
T2120 5786-5789 CC denotes and
T2121 5790-5791 DT denotes a
T2123 5792-5798 JJ denotes modest
T2122 5799-5808 NN denotes reduction
T2124 5809-5811 IN denotes in
T2125 5812-5820 NN denotes activity
T2126 5821-5827 IN denotes toward
T2127 5828-5837 NN denotes palmitoyl
T2129 5837-5838 HYPH denotes -
T2128 5838-5841 NN denotes CoA
T2130 5842-5850 VBN denotes compared
T2131 5851-5853 IN denotes to
T2132 5854-5858 NN denotes MCAD
T2134 5858-5859 SYM denotes +
T2135 5859-5860 HYPH denotes /
T2136 5860-5861 SYM denotes +
T2133 5862-5866 NNS denotes mice
T2137 5867-5868 -LRB- denotes (
T2138 5868-5873 NN denotes Table
T2139 5874-5875 CD denotes 1
T2140 5875-5876 -RRB- denotes )
T2141 5876-5877 . denotes .
T2142 5877-6051 sentence denotes Specifically, the dehydrogenation of octanoyl-CoA and palmitoyl-CoA substrates were reduced by 75% and by 30%, respectively, in MCAD−/− mice as compared to MCAD+/+ controls.
T2143 5878-5890 RB denotes Specifically
T2145 5890-5892 , denotes ,
T2146 5892-5895 DT denotes the
T2147 5896-5911 NN denotes dehydrogenation
T2148 5912-5914 IN denotes of
T2149 5915-5923 NN denotes octanoyl
T2151 5923-5924 HYPH denotes -
T2150 5924-5927 NN denotes CoA
T2153 5928-5931 CC denotes and
T2154 5932-5941 NN denotes palmitoyl
T2156 5941-5942 HYPH denotes -
T2155 5942-5945 NN denotes CoA
T2152 5946-5956 NNS denotes substrates
T2157 5957-5961 VBD denotes were
T2144 5962-5969 VBN denotes reduced
T2158 5970-5972 IN denotes by
T2159 5973-5975 CD denotes 75
T2160 5975-5976 NN denotes %
T2161 5977-5980 CC denotes and
T2162 5981-5983 IN denotes by
T2163 5984-5986 CD denotes 30
T2164 5986-5987 NN denotes %
T2165 5987-5989 , denotes ,
T2166 5989-6001 RB denotes respectively
T2167 6001-6003 , denotes ,
T2168 6003-6005 IN denotes in
T2169 6006-6010 NN denotes MCAD
T2171 6010-6011 SYM denotes −
T2172 6011-6012 HYPH denotes /
T2173 6012-6013 SYM denotes −
T2170 6014-6018 NNS denotes mice
T2174 6019-6021 IN denotes as
T2175 6022-6030 VBN denotes compared
T2176 6031-6033 IN denotes to
T2177 6034-6038 NN denotes MCAD
T2179 6038-6039 SYM denotes +
T2180 6039-6040 HYPH denotes /
T2181 6040-6041 SYM denotes +
T2178 6042-6050 NNS denotes controls
T2182 6050-6051 . denotes .
T8161 6061-6076 NNS denotes Characteristics
T8162 6077-6079 IN denotes of
T8163 6080-6084 NN denotes MCAD
T8165 6084-6085 HYPH denotes -
T8164 6085-6094 JJ denotes Deficient
T8166 6095-6099 NNS denotes Mice
T2294 6339-6347 JJ denotes Neonatal
T2295 6348-6354 NNS denotes Deaths
T2296 6354-6412 sentence denotes Significant neonatal mortality was noted in MCAD−/− pups.
T2297 6355-6366 JJ denotes Significant
T2299 6367-6375 JJ denotes neonatal
T2298 6376-6385 NN denotes mortality
T2301 6386-6389 VBD denotes was
T2300 6390-6395 VBN denotes noted
T2302 6396-6398 IN denotes in
T2303 6399-6403 NN denotes MCAD
T2305 6403-6404 SYM denotes −
T2306 6404-6405 HYPH denotes /
T2307 6405-6406 SYM denotes −
T2304 6407-6411 NNS denotes pups
T2308 6411-6412 . denotes .
T2309 6412-6575 sentence denotes Although equal numbers of pups were born from MCAD+/+ and MCAD−/− mice, only 41% of MCAD−/− mice survived to weaning as compared to 98% of MCAD+/+ mice (Table 1).
T2310 6413-6421 IN denotes Although
T2312 6422-6427 JJ denotes equal
T2313 6428-6435 NNS denotes numbers
T2314 6436-6438 IN denotes of
T2315 6439-6443 NNS denotes pups
T2316 6444-6448 VBD denotes were
T2311 6449-6453 VBN denotes born
T2318 6454-6458 IN denotes from
T2319 6459-6463 NN denotes MCAD
T2321 6463-6464 SYM denotes +
T2322 6464-6465 HYPH denotes /
T2323 6465-6466 SYM denotes +
T2324 6467-6470 CC denotes and
T2325 6471-6475 NN denotes MCAD
T2326 6475-6476 SYM denotes −
T2327 6476-6477 HYPH denotes /
T2328 6477-6478 SYM denotes −
T2320 6479-6483 NNS denotes mice
T2329 6483-6485 , denotes ,
T2330 6485-6489 RB denotes only
T2332 6490-6492 CD denotes 41
T2331 6492-6493 NN denotes %
T2333 6494-6496 IN denotes of
T2334 6497-6501 NN denotes MCAD
T2336 6501-6502 SYM denotes −
T2337 6502-6503 HYPH denotes /
T2338 6503-6504 SYM denotes −
T2335 6505-6509 NNS denotes mice
T2317 6510-6518 VBD denotes survived
T2339 6519-6521 IN denotes to
T2340 6522-6529 NN denotes weaning
T2341 6530-6532 IN denotes as
T2342 6533-6541 VBN denotes compared
T2343 6542-6544 IN denotes to
T2344 6545-6547 CD denotes 98
T2345 6547-6548 NN denotes %
T2346 6549-6551 IN denotes of
T2347 6552-6556 NN denotes MCAD
T2349 6556-6557 SYM denotes +
T2350 6557-6558 HYPH denotes /
T2351 6558-6559 SYM denotes +
T2348 6560-6564 NNS denotes mice
T2352 6565-6566 -LRB- denotes (
T2353 6566-6571 NN denotes Table
T2354 6572-6573 CD denotes 1
T2355 6573-6574 -RRB- denotes )
T2356 6574-6575 . denotes .
T2357 6575-6628 sentence denotes The mechanism of neonatal loss remains undetermined.
T2358 6576-6579 DT denotes The
T2359 6580-6589 NN denotes mechanism
T2361 6590-6592 IN denotes of
T2362 6593-6601 JJ denotes neonatal
T2363 6602-6606 NN denotes loss
T2360 6607-6614 VBZ denotes remains
T2364 6615-6627 JJ denotes undetermined
T2365 6627-6628 . denotes .
T2366 6628-6714 sentence denotes The MCAD−/− pups are abandoned more frequently than MCAD+/+ pups for unknown reasons.
T2367 6629-6632 DT denotes The
T2369 6633-6637 NN denotes MCAD
T2370 6637-6638 SYM denotes −
T2371 6638-6639 HYPH denotes /
T2372 6639-6640 SYM denotes −
T2368 6641-6645 NNS denotes pups
T2374 6646-6649 VBP denotes are
T2373 6650-6659 VBN denotes abandoned
T2375 6660-6664 RBR denotes more
T2376 6665-6675 RB denotes frequently
T2377 6676-6680 IN denotes than
T2378 6681-6685 NN denotes MCAD
T2380 6685-6686 SYM denotes +
T2381 6686-6687 HYPH denotes /
T2382 6687-6688 SYM denotes +
T2379 6689-6693 NNS denotes pups
T2383 6694-6697 IN denotes for
T2384 6698-6705 JJ denotes unknown
T2385 6706-6713 NNS denotes reasons
T2386 6713-6714 . denotes .
T2387 6714-6770 sentence denotes They are likely more prone to hypothermia than MCAD+/+.
T2388 6715-6719 PRP denotes They
T2389 6720-6723 VBP denotes are
T2390 6724-6730 RB denotes likely
T2391 6731-6735 RBR denotes more
T2392 6736-6741 JJ denotes prone
T2393 6742-6744 IN denotes to
T2394 6745-6756 NN denotes hypothermia
T2395 6757-6761 IN denotes than
T2396 6762-6766 NN denotes MCAD
T2397 6766-6767 SYM denotes +
T2398 6767-6768 HYPH denotes /
T2399 6768-6769 SYM denotes +
T2400 6769-6770 . denotes .
T2401 6770-7002 sentence denotes Because of the difficulty of distinguishing the MCAD−/− mutants from the MCAD+/− heterozygous pups by molecular analysis due to the insertion mutation, we could only compare MCAD+/+ × MCAD+/+ matings with MCAD−/− × MCAD−/− matings.
T2402 6771-6778 IN denotes Because
T2404 6779-6781 IN denotes of
T2405 6782-6785 DT denotes the
T2406 6786-6796 NN denotes difficulty
T2407 6797-6799 IN denotes of
T2408 6800-6814 VBG denotes distinguishing
T2409 6815-6818 DT denotes the
T2411 6819-6823 NN denotes MCAD
T2412 6823-6824 SYM denotes −
T2413 6824-6825 HYPH denotes /
T2414 6825-6826 SYM denotes −
T2410 6827-6834 NNS denotes mutants
T2415 6835-6839 IN denotes from
T2416 6840-6843 DT denotes the
T2418 6844-6848 NN denotes MCAD
T2419 6848-6849 SYM denotes +
T2420 6849-6850 HYPH denotes /
T2421 6850-6851 SYM denotes −
T2422 6852-6864 JJ denotes heterozygous
T2417 6865-6869 NNS denotes pups
T2423 6870-6872 IN denotes by
T2424 6873-6882 JJ denotes molecular
T2425 6883-6891 NN denotes analysis
T2426 6892-6895 IN denotes due
T2427 6896-6898 IN denotes to
T2428 6899-6902 DT denotes the
T2430 6903-6912 NN denotes insertion
T2429 6913-6921 NN denotes mutation
T2431 6921-6923 , denotes ,
T2432 6923-6925 PRP denotes we
T2433 6926-6931 MD denotes could
T2434 6932-6936 RB denotes only
T2403 6937-6944 VB denotes compare
T2435 6945-6949 NN denotes MCAD
T2437 6949-6950 SYM denotes +
T2438 6950-6951 HYPH denotes /
T2439 6951-6952 SYM denotes +
T2440 6953-6954 SYM denotes ×
T2441 6955-6959 NN denotes MCAD
T2442 6959-6960 SYM denotes +
T2443 6960-6961 HYPH denotes /
T2444 6961-6962 SYM denotes +
T2436 6963-6970 NNS denotes matings
T2445 6971-6975 IN denotes with
T2446 6976-6980 NN denotes MCAD
T2448 6980-6981 SYM denotes −
T2449 6981-6982 HYPH denotes /
T2450 6982-6983 SYM denotes −
T2451 6984-6985 SYM denotes ×
T2452 6986-6990 NN denotes MCAD
T2453 6990-6991 SYM denotes −
T2454 6991-6992 HYPH denotes /
T2455 6992-6993 SYM denotes −
T2447 6994-7001 NNS denotes matings
T2456 7001-7002 . denotes .
T2457 7002-7076 sentence denotes Thus, we were unable to evaluate the pedigrees from heterozygous matings.
T2458 7003-7007 RB denotes Thus
T2460 7007-7009 , denotes ,
T2461 7009-7011 PRP denotes we
T2459 7012-7016 VBD denotes were
T2462 7017-7023 JJ denotes unable
T2463 7024-7026 TO denotes to
T2464 7027-7035 VB denotes evaluate
T2465 7036-7039 DT denotes the
T2466 7040-7049 NNS denotes pedigrees
T2467 7050-7054 IN denotes from
T2468 7055-7067 JJ denotes heterozygous
T2469 7068-7075 NNS denotes matings
T2470 7075-7076 . denotes .
T2565 7078-7085 NN denotes Fasting
T2566 7086-7089 CC denotes and
T2567 7090-7094 NN denotes Cold
T2568 7095-7106 NN denotes Intolerance
T2569 7106-7225 sentence denotes In order to examine the stress effects of fasting on MCAD-deficient mice, they were fasted for 24 h prior to analysis.
T2570 7107-7109 IN denotes In
T2572 7110-7115 NN denotes order
T2573 7116-7118 TO denotes to
T2574 7119-7126 VB denotes examine
T2575 7127-7130 DT denotes the
T2577 7131-7137 NN denotes stress
T2576 7138-7145 NNS denotes effects
T2578 7146-7148 IN denotes of
T2579 7149-7156 NN denotes fasting
T2580 7157-7159 IN denotes on
T2581 7160-7164 NN denotes MCAD
T2583 7164-7165 HYPH denotes -
T2582 7165-7174 JJ denotes deficient
T2584 7175-7179 NNS denotes mice
T2585 7179-7181 , denotes ,
T2586 7181-7185 PRP denotes they
T2587 7186-7190 VBD denotes were
T2571 7191-7197 VBN denotes fasted
T2588 7198-7201 IN denotes for
T2589 7202-7204 CD denotes 24
T2590 7205-7206 NN denotes h
T2591 7207-7212 JJ denotes prior
T2592 7213-7215 IN denotes to
T2593 7216-7224 NN denotes analysis
T2594 7224-7225 . denotes .
T2595 7225-7390 sentence denotes MCAD−/− mice displayed lower serum glucose and elevated serum free fatty acid levels although neither result was significant, as compared to MCAD+/+ mice (Table 1).
T2596 7226-7230 NN denotes MCAD
T2598 7230-7231 SYM denotes −
T2599 7231-7232 HYPH denotes /
T2600 7232-7233 SYM denotes −
T2597 7234-7238 NNS denotes mice
T2601 7239-7248 VBD denotes displayed
T2602 7249-7254 JJR denotes lower
T2604 7255-7260 NN denotes serum
T2603 7261-7268 NN denotes glucose
T2605 7269-7272 CC denotes and
T2606 7273-7281 VBN denotes elevated
T2608 7282-7287 NN denotes serum
T2609 7288-7292 JJ denotes free
T2611 7293-7298 JJ denotes fatty
T2610 7299-7303 NN denotes acid
T2607 7304-7310 NNS denotes levels
T2612 7311-7319 IN denotes although
T2614 7320-7327 DT denotes neither
T2615 7328-7334 NN denotes result
T2613 7335-7338 VBD denotes was
T2616 7339-7350 JJ denotes significant
T2617 7350-7352 , denotes ,
T2618 7352-7354 IN denotes as
T2619 7355-7363 VBN denotes compared
T2620 7364-7366 IN denotes to
T2621 7367-7371 NN denotes MCAD
T2623 7371-7372 SYM denotes +
T2624 7372-7373 HYPH denotes /
T2625 7373-7374 SYM denotes +
T2622 7375-7379 NNS denotes mice
T2626 7380-7381 -LRB- denotes (
T2627 7381-7386 NN denotes Table
T2628 7387-7388 CD denotes 1
T2629 7388-7389 -RRB- denotes )
T2630 7389-7390 . denotes .
T2631 7390-7506 sentence denotes To determine the effects of cold stress, mice were fasted for 18 h and placed in 4 °C environment for a 3-h period.
T2632 7391-7393 TO denotes To
T2633 7394-7403 VB denotes determine
T2635 7404-7407 DT denotes the
T2636 7408-7415 NNS denotes effects
T2637 7416-7418 IN denotes of
T2638 7419-7423 NN denotes cold
T2639 7424-7430 NN denotes stress
T2640 7430-7432 , denotes ,
T2641 7432-7436 NNS denotes mice
T2642 7437-7441 VBD denotes were
T2634 7442-7448 VBN denotes fasted
T2643 7449-7452 IN denotes for
T2644 7453-7455 CD denotes 18
T2645 7456-7457 NN denotes h
T2646 7458-7461 CC denotes and
T2647 7462-7468 VBN denotes placed
T2648 7469-7471 IN denotes in
T2649 7472-7473 CD denotes 4
T2650 7474-7476 NN denotes °C
T2651 7477-7488 NN denotes environment
T2652 7489-7492 IN denotes for
T2653 7493-7494 DT denotes a
T2655 7495-7496 CD denotes 3
T2657 7496-7497 HYPH denotes -
T2656 7497-7498 NN denotes h
T2654 7499-7505 NN denotes period
T2658 7505-7506 . denotes .
T2659 7506-7639 sentence denotes The MCAD−/− mice were significantly compromised within this short period of cold stress, some severe enough to result in fatalities.
T2660 7507-7510 DT denotes The
T2662 7511-7515 NN denotes MCAD
T2663 7515-7516 SYM denotes −
T2664 7516-7517 HYPH denotes /
T2665 7517-7518 SYM denotes −
T2661 7519-7523 NNS denotes mice
T2667 7524-7528 VBD denotes were
T2668 7529-7542 RB denotes significantly
T2666 7543-7554 VBN denotes compromised
T2669 7555-7561 IN denotes within
T2670 7562-7566 DT denotes this
T2672 7567-7572 JJ denotes short
T2671 7573-7579 NN denotes period
T2673 7580-7582 IN denotes of
T2674 7583-7587 NN denotes cold
T2675 7588-7594 NN denotes stress
T2676 7594-7596 , denotes ,
T2677 7596-7600 DT denotes some
T2678 7601-7607 JJ denotes severe
T2679 7608-7614 RB denotes enough
T2680 7615-7617 TO denotes to
T2681 7618-7624 VB denotes result
T2682 7625-7627 IN denotes in
T2683 7628-7638 NNS denotes fatalities
T2684 7638-7639 . denotes .
T2685 7639-7788 sentence denotes After 1 h of the cold challenge, the average rectal temperature of MCAD−/− mice (n = 5) was 23.4 °C as compared with 35 °C for MCAD+/+ mice (n = 4).
T2686 7640-7645 IN denotes After
T2688 7646-7647 CD denotes 1
T2689 7648-7649 NN denotes h
T2690 7650-7652 IN denotes of
T2691 7653-7656 DT denotes the
T2693 7657-7661 NN denotes cold
T2692 7662-7671 NN denotes challenge
T2694 7671-7673 , denotes ,
T2695 7673-7676 DT denotes the
T2697 7677-7684 JJ denotes average
T2698 7685-7691 JJ denotes rectal
T2696 7692-7703 NN denotes temperature
T2699 7704-7706 IN denotes of
T2700 7707-7711 NN denotes MCAD
T2702 7711-7712 SYM denotes −
T2703 7712-7713 HYPH denotes /
T2704 7713-7714 SYM denotes −
T2701 7715-7719 NNS denotes mice
T2705 7720-7721 -LRB- denotes (
T2707 7721-7722 NN denotes n
T2708 7723-7724 SYM denotes =
T2706 7725-7726 CD denotes 5
T2709 7726-7727 -RRB- denotes )
T2687 7728-7731 VBD denotes was
T2710 7732-7736 CD denotes 23.4
T2711 7737-7739 NN denotes °C
T2712 7740-7742 IN denotes as
T2713 7743-7751 VBN denotes compared
T2714 7752-7756 IN denotes with
T2715 7757-7759 CD denotes 35
T2716 7760-7762 NN denotes °C
T2717 7763-7766 IN denotes for
T2718 7767-7771 NN denotes MCAD
T2720 7771-7772 SYM denotes +
T2721 7772-7773 HYPH denotes /
T2722 7773-7774 SYM denotes +
T2719 7775-7779 NNS denotes mice
T2723 7780-7781 -LRB- denotes (
T2725 7781-7782 NN denotes n
T2726 7783-7784 SYM denotes =
T2724 7785-7786 CD denotes 4
T2727 7786-7787 -RRB- denotes )
T2728 7787-7788 . denotes .
T2729 7788-7898 sentence denotes Rectal temperatures declined to unrecoverable temperatures of 16.7–19.2 °C in three of the five MCAD−/− mice.
T2730 7789-7795 JJ denotes Rectal
T2731 7796-7808 NNS denotes temperatures
T2732 7809-7817 VBD denotes declined
T2733 7818-7820 IN denotes to
T2734 7821-7834 JJ denotes unrecoverable
T2735 7835-7847 NNS denotes temperatures
T2736 7848-7850 IN denotes of
T2737 7851-7855 CD denotes 16.7
T2739 7855-7856 SYM denotes –
T2738 7856-7860 CD denotes 19.2
T2740 7861-7863 NN denotes °C
T2741 7864-7866 IN denotes in
T2742 7867-7872 CD denotes three
T2744 7873-7875 IN denotes of
T2745 7876-7879 DT denotes the
T2743 7880-7884 CD denotes five
T2747 7885-7889 NN denotes MCAD
T2748 7889-7890 SYM denotes −
T2749 7890-7891 HYPH denotes /
T2750 7891-7892 SYM denotes −
T2746 7893-7897 NNS denotes mice
T2751 7897-7898 . denotes .
T2752 7898-7977 sentence denotes By the end of the 1.5-h mark, the two surviving MCAD−/− mice averaged 22.7 °C.
T2753 7899-7901 IN denotes By
T2755 7902-7905 DT denotes the
T2756 7906-7909 NN denotes end
T2757 7910-7912 IN denotes of
T2758 7913-7916 DT denotes the
T2760 7917-7920 CD denotes 1.5
T2762 7920-7921 HYPH denotes -
T2761 7921-7922 NN denotes h
T2759 7923-7927 NN denotes mark
T2763 7927-7929 , denotes ,
T2764 7929-7932 DT denotes the
T2766 7933-7936 CD denotes two
T2767 7937-7946 VBG denotes surviving
T2768 7947-7951 NN denotes MCAD
T2769 7951-7952 SYM denotes −
T2770 7952-7953 HYPH denotes /
T2771 7953-7954 SYM denotes −
T2765 7955-7959 NNS denotes mice
T2754 7960-7968 VBD denotes averaged
T2772 7969-7973 CD denotes 22.7
T2773 7974-7976 NN denotes °C
T2774 7976-7977 . denotes .
T2775 7977-8096 sentence denotes In contrast, all four MCAD+/+ mice survived the 3-h cold stress, ending with an average rectal temperature of 33.6 °C.
T2776 7978-7980 IN denotes In
T2778 7981-7989 NN denotes contrast
T2779 7989-7991 , denotes ,
T2780 7991-7994 DT denotes all
T2782 7995-7999 CD denotes four
T2783 8000-8004 NN denotes MCAD
T2784 8004-8005 SYM denotes +
T2785 8005-8006 HYPH denotes /
T2786 8006-8007 SYM denotes +
T2781 8008-8012 NNS denotes mice
T2777 8013-8021 VBD denotes survived
T2787 8022-8025 DT denotes the
T2789 8026-8027 CD denotes 3
T2791 8027-8028 HYPH denotes -
T2790 8028-8029 NN denotes h
T2792 8030-8034 NN denotes cold
T2788 8035-8041 NN denotes stress
T2793 8041-8043 , denotes ,
T2794 8043-8049 VBG denotes ending
T2795 8050-8054 IN denotes with
T2796 8055-8057 DT denotes an
T2798 8058-8065 JJ denotes average
T2799 8066-8072 JJ denotes rectal
T2797 8073-8084 NN denotes temperature
T2800 8085-8087 IN denotes of
T2801 8088-8092 CD denotes 33.6
T2802 8093-8095 NN denotes °C
T2803 8095-8096 . denotes .
T2908 8098-8105 JJ denotes Organic
T2909 8106-8110 NN denotes Acid
T2910 8111-8114 CC denotes and
T2911 8115-8128 NN denotes Acylcarnitine
T2912 8129-8137 NN denotes Analysis
T2913 8137-8268 sentence denotes Urine organic acid analysis revealed that MCAD−/− mice developed an organic acid profile similar to MCAD-deficient human patients.
T2914 8138-8143 NN denotes Urine
T2916 8144-8151 JJ denotes organic
T2915 8152-8156 NN denotes acid
T2917 8157-8165 NN denotes analysis
T2918 8166-8174 VBD denotes revealed
T2919 8175-8179 IN denotes that
T2921 8180-8184 NN denotes MCAD
T2923 8184-8185 SYM denotes −
T2924 8185-8186 HYPH denotes /
T2925 8186-8187 SYM denotes −
T2922 8188-8192 NNS denotes mice
T2920 8193-8202 VBD denotes developed
T2926 8203-8205 DT denotes an
T2928 8206-8213 JJ denotes organic
T2929 8214-8218 NN denotes acid
T2927 8219-8226 NN denotes profile
T2930 8227-8234 JJ denotes similar
T2931 8235-8237 IN denotes to
T2932 8238-8242 NN denotes MCAD
T2934 8242-8243 HYPH denotes -
T2933 8243-8252 JJ denotes deficient
T2936 8253-8258 JJ denotes human
T2935 8259-8267 NNS denotes patients
T2937 8267-8268 . denotes .
T2938 8268-8537 sentence denotes Specifically, when fasted for 18 h, MCAD−/− mice developed significantly elevated concentrations of adipic, suberic, and sebacic acids and hexanoylglycine as compared to MCAD+/+ controls, which showed trace to no detectable amounts of the same organic acids (Table 1).
T2939 8269-8281 RB denotes Specifically
T2941 8281-8283 , denotes ,
T2942 8283-8287 WRB denotes when
T2943 8288-8294 VBN denotes fasted
T2944 8295-8298 IN denotes for
T2945 8299-8301 CD denotes 18
T2946 8302-8303 NN denotes h
T2947 8303-8305 , denotes ,
T2948 8305-8309 NN denotes MCAD
T2950 8309-8310 SYM denotes −
T2951 8310-8311 HYPH denotes /
T2952 8311-8312 SYM denotes −
T2949 8313-8317 NNS denotes mice
T2940 8318-8327 VBD denotes developed
T2953 8328-8341 RB denotes significantly
T2954 8342-8350 VBN denotes elevated
T2955 8351-8365 NNS denotes concentrations
T2956 8366-8368 IN denotes of
T2957 8369-8375 JJ denotes adipic
T2959 8375-8377 , denotes ,
T2960 8377-8384 JJ denotes suberic
T2961 8384-8386 , denotes ,
T2962 8386-8389 CC denotes and
T2963 8390-8397 JJ denotes sebacic
T2958 8398-8403 NNS denotes acids
T2964 8404-8407 CC denotes and
T2965 8408-8423 NN denotes hexanoylglycine
T2966 8424-8426 IN denotes as
T2967 8427-8435 VBN denotes compared
T2968 8436-8438 IN denotes to
T2969 8439-8443 NN denotes MCAD
T2971 8443-8444 SYM denotes +
T2972 8444-8445 HYPH denotes /
T2973 8445-8446 SYM denotes +
T2970 8447-8455 NNS denotes controls
T2974 8455-8457 , denotes ,
T2975 8457-8462 WDT denotes which
T2976 8463-8469 VBD denotes showed
T2977 8470-8475 NN denotes trace
T2978 8476-8478 IN denotes to
T2979 8479-8481 DT denotes no
T2981 8482-8492 JJ denotes detectable
T2980 8493-8500 NNS denotes amounts
T2982 8501-8503 IN denotes of
T2983 8504-8507 DT denotes the
T2985 8508-8512 JJ denotes same
T2986 8513-8520 JJ denotes organic
T2984 8521-8526 NNS denotes acids
T2987 8527-8528 -LRB- denotes (
T2988 8528-8533 NN denotes Table
T2989 8534-8535 CD denotes 1
T2990 8535-8536 -RRB- denotes )
T2991 8536-8537 . denotes .
T2992 8537-8585 sentence denotes Adipic acid is not specific to MCAD deficiency.
T2993 8538-8544 JJ denotes Adipic
T2994 8545-8549 NN denotes acid
T2995 8550-8552 VBZ denotes is
T2996 8553-8556 RB denotes not
T2997 8557-8565 JJ denotes specific
T2998 8566-8568 IN denotes to
T2999 8569-8573 NN denotes MCAD
T3000 8574-8584 NN denotes deficiency
T3001 8584-8585 . denotes .
T3002 8585-8729 sentence denotes We also evaluated β-hydroxybutyric and acetoacetic concentrations and found no significant differences between MCAD genotypes (data not shown).
T3003 8586-8588 PRP denotes We
T3005 8589-8593 RB denotes also
T3004 8594-8603 VBD denotes evaluated
T3006 8604-8605 NN denotes β
T3008 8605-8606 HYPH denotes -
T3007 8606-8620 JJ denotes hydroxybutyric
T3010 8621-8624 CC denotes and
T3011 8625-8636 JJ denotes acetoacetic
T3009 8637-8651 NNS denotes concentrations
T3012 8652-8655 CC denotes and
T3013 8656-8661 VBD denotes found
T3014 8662-8664 DT denotes no
T3016 8665-8676 JJ denotes significant
T3015 8677-8688 NNS denotes differences
T3017 8689-8696 IN denotes between
T3018 8697-8701 NN denotes MCAD
T3019 8702-8711 NNS denotes genotypes
T3020 8712-8713 -LRB- denotes (
T3022 8713-8717 NNS denotes data
T3023 8718-8721 RB denotes not
T3021 8722-8727 VBN denotes shown
T3024 8727-8728 -RRB- denotes )
T3025 8728-8729 . denotes .
T3026 8729-9001 sentence denotes Comparison of MCAD+/+ and MCAD−/− mice revealed no significant differences in total serum carnitine concentrations between MCAD+/+ and MCAD−/− mice, but MCAD−/− mice had a 5- to 6-fold elevation of serum decenoylcarnitine evident in the acylcarnitine profile (Figure 4A).
T3027 8730-8740 NN denotes Comparison
T3029 8741-8743 IN denotes of
T3030 8744-8748 NN denotes MCAD
T3032 8748-8749 SYM denotes +
T3033 8749-8750 HYPH denotes /
T3034 8750-8751 SYM denotes +
T3035 8752-8755 CC denotes and
T3036 8756-8760 NN denotes MCAD
T3037 8760-8761 SYM denotes −
T3038 8761-8762 HYPH denotes /
T3039 8762-8763 SYM denotes −
T3031 8764-8768 NNS denotes mice
T3028 8769-8777 VBD denotes revealed
T3040 8778-8780 DT denotes no
T3042 8781-8792 JJ denotes significant
T3041 8793-8804 NNS denotes differences
T3043 8805-8807 IN denotes in
T3044 8808-8813 JJ denotes total
T3046 8814-8819 NN denotes serum
T3047 8820-8829 NN denotes carnitine
T3045 8830-8844 NNS denotes concentrations
T3048 8845-8852 IN denotes between
T3049 8853-8857 NN denotes MCAD
T3051 8857-8858 SYM denotes +
T3052 8858-8859 HYPH denotes /
T3053 8859-8860 SYM denotes +
T3054 8861-8864 CC denotes and
T3055 8865-8869 NN denotes MCAD
T3056 8869-8870 SYM denotes −
T3057 8870-8871 HYPH denotes /
T3058 8871-8872 SYM denotes −
T3050 8873-8877 NNS denotes mice
T3059 8877-8879 , denotes ,
T3060 8879-8882 CC denotes but
T3061 8883-8887 NN denotes MCAD
T3063 8887-8888 SYM denotes −
T3064 8888-8889 HYPH denotes /
T3065 8889-8890 SYM denotes −
T3062 8891-8895 NNS denotes mice
T3066 8896-8899 VBD denotes had
T3067 8900-8901 DT denotes a
T3069 8902-8903 CD denotes 5
T3071 8903-8904 HYPH denotes -
T3072 8905-8907 IN denotes to
T3070 8908-8909 CD denotes 6
T3074 8909-8910 HYPH denotes -
T3073 8910-8914 JJ denotes fold
T3068 8915-8924 NN denotes elevation
T3076 8925-8927 IN denotes of
T3077 8928-8933 NN denotes serum
T3078 8934-8951 NN denotes decenoylcarnitine
T3075 8952-8959 JJ denotes evident
T3079 8960-8962 IN denotes in
T3080 8963-8966 DT denotes the
T3082 8967-8980 NN denotes acylcarnitine
T3081 8981-8988 NN denotes profile
T3083 8989-8990 -LRB- denotes (
T3084 8990-8996 NN denotes Figure
T3085 8997-8999 CD denotes 4A
T3086 8999-9000 -RRB- denotes )
T3087 9000-9001 . denotes .
T3088 9001-9095 sentence denotes Bile acylcarnitine analysis revealed a similar acylcarnitine pattern as in serum (Figure 4B).
T3089 9002-9006 NN denotes Bile
T3090 9007-9020 NN denotes acylcarnitine
T3091 9021-9029 NN denotes analysis
T3092 9030-9038 VBD denotes revealed
T3093 9039-9040 DT denotes a
T3095 9041-9048 JJ denotes similar
T3096 9049-9062 NN denotes acylcarnitine
T3094 9063-9070 NN denotes pattern
T3097 9071-9073 IN denotes as
T3098 9074-9076 IN denotes in
T3099 9077-9082 NN denotes serum
T3100 9083-9084 -LRB- denotes (
T3101 9084-9090 NN denotes Figure
T3102 9091-9093 CD denotes 4B
T3103 9093-9094 -RRB- denotes )
T3104 9094-9095 . denotes .
T3105 9095-9222 sentence denotes However, the acylcarnitine profiles of the MCAD−/− mice are different from those of human MCAD-deficient patients (Figure 4C).
T3106 9096-9103 RB denotes However
T3108 9103-9105 , denotes ,
T3109 9105-9108 DT denotes the
T3111 9109-9122 NN denotes acylcarnitine
T3110 9123-9131 NNS denotes profiles
T3112 9132-9134 IN denotes of
T3113 9135-9138 DT denotes the
T3115 9139-9143 NN denotes MCAD
T3116 9143-9144 SYM denotes −
T3117 9144-9145 HYPH denotes /
T3118 9145-9146 SYM denotes −
T3114 9147-9151 NNS denotes mice
T3107 9152-9155 VBP denotes are
T3119 9156-9165 JJ denotes different
T3120 9166-9170 IN denotes from
T3121 9171-9176 DT denotes those
T3122 9177-9179 IN denotes of
T3123 9180-9185 JJ denotes human
T3125 9186-9190 NN denotes MCAD
T3127 9190-9191 HYPH denotes -
T3126 9191-9200 JJ denotes deficient
T3124 9201-9209 NNS denotes patients
T3128 9210-9211 -LRB- denotes (
T3129 9211-9217 NN denotes Figure
T3130 9218-9220 CD denotes 4C
T3131 9220-9221 -RRB- denotes )
T3132 9221-9222 . denotes .
T3133 9222-9438 sentence denotes Human patients present with elevated levels of C6, C8, and C10:1 acylcarnitines, as did the mutant mice; however, the predominant peak was C8 acylcarnitine in humans, whereas in the mouse it was C10:1 acylcarnitine.
T3134 9223-9228 JJ denotes Human
T3135 9229-9237 NNS denotes patients
T3136 9238-9245 VBP denotes present
T3138 9246-9250 IN denotes with
T3139 9251-9259 VBN denotes elevated
T3140 9260-9266 NNS denotes levels
T3141 9267-9269 IN denotes of
T3142 9270-9272 NN denotes C6
T3143 9272-9274 , denotes ,
T3144 9274-9276 NN denotes C8
T3145 9276-9278 , denotes ,
T3146 9278-9281 CC denotes and
T3147 9282-9285 NN denotes C10
T3148 9285-9286 : denotes :
T3149 9286-9287 CD denotes 1
T3150 9288-9302 NNS denotes acylcarnitines
T3151 9302-9304 , denotes ,
T3152 9304-9306 IN denotes as
T3153 9307-9310 VBD denotes did
T3154 9311-9314 DT denotes the
T3156 9315-9321 NN denotes mutant
T3155 9322-9326 NNS denotes mice
T3157 9326-9327 : denotes ;
T3158 9328-9335 RB denotes however
T3159 9335-9337 , denotes ,
T3160 9337-9340 DT denotes the
T3162 9341-9352 JJ denotes predominant
T3161 9353-9357 NN denotes peak
T3137 9358-9361 VBD denotes was
T3163 9362-9364 NN denotes C8
T3164 9365-9378 NN denotes acylcarnitine
T3165 9379-9381 IN denotes in
T3166 9382-9388 NNS denotes humans
T3167 9388-9390 , denotes ,
T3168 9390-9397 IN denotes whereas
T3170 9398-9400 IN denotes in
T3171 9401-9404 DT denotes the
T3172 9405-9410 NN denotes mouse
T3173 9411-9413 PRP denotes it
T3169 9414-9417 VBD denotes was
T3174 9418-9421 NN denotes C10
T3176 9421-9422 : denotes :
T3177 9422-9423 CD denotes 1
T3175 9424-9437 NN denotes acylcarnitine
T3178 9437-9438 . denotes .
T7814 9449-9462 NN denotes Acylcarnitine
T7815 9463-9471 NNS denotes Analyses
T7816 9471-9548 sentence denotes (A) Serum acylcarnitine analysis of MCAD+/+ (n = 4) and MCAD−/− mice (n = 4)
T7817 9472-9473 -LRB- denotes (
T7818 9473-9474 LS denotes A
T7820 9474-9475 -RRB- denotes )
T7821 9476-9481 NN denotes Serum
T7822 9482-9495 NN denotes acylcarnitine
T7819 9496-9504 NN denotes analysis
T7823 9505-9507 IN denotes of
T7824 9508-9512 NN denotes MCAD
T7826 9512-9513 SYM denotes +
T7827 9513-9514 HYPH denotes /
T7828 9514-9515 SYM denotes +
T7829 9516-9517 -LRB- denotes (
T7831 9517-9518 NN denotes n
T7832 9519-9520 SYM denotes =
T7830 9521-9522 CD denotes 4
T7833 9522-9523 -RRB- denotes )
T7834 9524-9527 CC denotes and
T7835 9528-9532 NN denotes MCAD
T7836 9532-9533 SYM denotes −
T7837 9533-9534 HYPH denotes /
T7838 9534-9535 SYM denotes −
T7825 9536-9540 NNS denotes mice
T7839 9541-9542 -LRB- denotes (
T7841 9542-9543 NN denotes n
T7842 9544-9545 SYM denotes =
T7840 9546-9547 CD denotes 4
T7843 9547-9548 -RRB- denotes )
T7844 9548-9636 sentence denotes There are significant elevations in acylcarnitine species as indicated in MCAD−/− mice.
T7845 9549-9554 EX denotes There
T7846 9555-9558 VBP denotes are
T7847 9559-9570 JJ denotes significant
T7848 9571-9581 NNS denotes elevations
T7849 9582-9584 IN denotes in
T7850 9585-9598 NN denotes acylcarnitine
T7851 9599-9606 NNS denotes species
T7852 9607-9609 IN denotes as
T7853 9610-9619 VBN denotes indicated
T7854 9620-9622 IN denotes in
T7855 9623-9627 NN denotes MCAD
T7857 9627-9628 SYM denotes −
T7858 9628-9629 HYPH denotes /
T7859 9629-9630 SYM denotes −
T7856 9631-9635 NNS denotes mice
T7860 9635-9636 . denotes .
T7861 9636-9692 sentence denotes Values shown are mean values ± standard deviation (SD).
T7862 9637-9643 NNS denotes Values
T7864 9644-9649 VBN denotes shown
T7863 9650-9653 VBP denotes are
T7865 9654-9658 NN denotes mean
T7866 9659-9665 NNS denotes values
T7867 9666-9667 SYM denotes ±
T7868 9668-9676 JJ denotes standard
T7869 9677-9686 NN denotes deviation
T7870 9687-9688 -LRB- denotes (
T7871 9688-9690 NN denotes SD
T7872 9690-9691 -RRB- denotes )
T7873 9691-9692 . denotes .
T7874 9692-9747 sentence denotes Asterisk indicates p < 0.002 and ‡ indicates p < 0.01.
T7875 9693-9701 NN denotes Asterisk
T7876 9702-9711 VBZ denotes indicates
T7877 9712-9713 NN denotes p
T7878 9714-9715 SYM denotes <
T7879 9716-9721 CD denotes 0.002
T7880 9722-9725 CC denotes and
T7881 9726-9727 SYM denotes ‡
T7882 9728-9737 VBZ denotes indicates
T7883 9738-9739 NN denotes p
T7884 9740-9741 SYM denotes <
T7885 9742-9746 CD denotes 0.01
T7886 9746-9747 . denotes .
T7887 9747-9851 sentence denotes (B) There are significant elevations in bile acylcarnitines of the same mice shown in (A) as indicated.
T7888 9748-9749 -LRB- denotes (
T7889 9749-9750 LS denotes B
T7891 9750-9751 -RRB- denotes )
T7892 9752-9757 EX denotes There
T7890 9758-9761 VBP denotes are
T7893 9762-9773 JJ denotes significant
T7894 9774-9784 NNS denotes elevations
T7895 9785-9787 IN denotes in
T7896 9788-9792 NN denotes bile
T7897 9793-9807 NNS denotes acylcarnitines
T7898 9808-9810 IN denotes of
T7899 9811-9814 DT denotes the
T7901 9815-9819 JJ denotes same
T7900 9820-9824 NNS denotes mice
T7902 9825-9830 VBN denotes shown
T7903 9831-9833 IN denotes in
T7904 9834-9835 -LRB- denotes (
T7905 9835-9836 NN denotes A
T7906 9836-9837 -RRB- denotes )
T7907 9838-9840 IN denotes as
T7908 9841-9850 VBN denotes indicated
T7909 9850-9851 . denotes .
T7910 9851-9886 sentence denotes Values shown are mean values ± SD.
T7911 9852-9858 NNS denotes Values
T7913 9859-9864 VBN denotes shown
T7912 9865-9868 VBP denotes are
T7914 9869-9873 NN denotes mean
T7915 9874-9880 NNS denotes values
T7916 9881-9882 SYM denotes ±
T7917 9883-9885 NN denotes SD
T7918 9885-9886 . denotes .
T7919 9886-9916 sentence denotes Asterisk indicates p < 0.001.
T7920 9887-9895 NN denotes Asterisk
T7921 9896-9905 VBZ denotes indicates
T7922 9906-9907 NN denotes p
T7923 9908-9909 SYM denotes <
T7924 9910-9915 CD denotes 0.001
T7925 9915-9916 . denotes .
T7926 9916-10017 sentence denotes (C) Bile acylcarnitine profile of an MCAD−/− mouse compared to a human patient with MCAD deficiency.
T7927 9917-9918 -LRB- denotes (
T7928 9918-9919 LS denotes C
T7930 9919-9920 -RRB- denotes )
T7931 9921-9925 NN denotes Bile
T7932 9926-9939 NN denotes acylcarnitine
T7929 9940-9947 NN denotes profile
T7933 9948-9950 IN denotes of
T7934 9951-9953 DT denotes an
T7936 9954-9958 NN denotes MCAD
T7937 9958-9959 SYM denotes −
T7938 9959-9960 HYPH denotes /
T7939 9960-9961 SYM denotes −
T7935 9962-9967 NN denotes mouse
T7940 9968-9976 VBN denotes compared
T7941 9977-9979 IN denotes to
T7942 9980-9981 DT denotes a
T7944 9982-9987 JJ denotes human
T7943 9988-9995 NN denotes patient
T7945 9996-10000 IN denotes with
T7946 10001-10005 NN denotes MCAD
T7947 10006-10016 NN denotes deficiency
T7948 10016-10017 . denotes .
T7949 10017-10066 sentence denotes Internal standards are indicated by an asterisk.
T7950 10018-10026 JJ denotes Internal
T7951 10027-10036 NNS denotes standards
T7953 10037-10040 VBP denotes are
T7952 10041-10050 VBN denotes indicated
T7954 10051-10053 IN denotes by
T7955 10054-10056 DT denotes an
T7956 10057-10065 NN denotes asterisk
T7957 10065-10066 . denotes .
T3317 10068-10082 NN denotes Histopathology
T3318 10082-10352 sentence denotes Complete histopathologic examination of one group of mutant and MCAD+/+ control mice after a 24-h fast demonstrated diffuse microvesicular and macrovesicular hepatic steatosis in 6–8-wk-old MCAD−/− mice whereas MCAD+/+ mice had no histologic changes (Figure 5A and 5B).
T3319 10083-10091 JJ denotes Complete
T3321 10092-10107 JJ denotes histopathologic
T3320 10108-10119 NN denotes examination
T3323 10120-10122 IN denotes of
T3324 10123-10126 CD denotes one
T3325 10127-10132 NN denotes group
T3326 10133-10135 IN denotes of
T3327 10136-10142 NN denotes mutant
T3329 10143-10146 CC denotes and
T3330 10147-10151 NN denotes MCAD
T3331 10151-10152 SYM denotes +
T3332 10152-10153 HYPH denotes /
T3333 10153-10154 SYM denotes +
T3334 10155-10162 NN denotes control
T3328 10163-10167 NNS denotes mice
T3335 10168-10173 IN denotes after
T3336 10174-10175 DT denotes a
T3338 10176-10178 CD denotes 24
T3340 10178-10179 HYPH denotes -
T3339 10179-10180 NN denotes h
T3337 10181-10185 NN denotes fast
T3322 10186-10198 VBD denotes demonstrated
T3341 10199-10206 JJ denotes diffuse
T3343 10207-10221 JJ denotes microvesicular
T3344 10222-10225 CC denotes and
T3345 10226-10240 JJ denotes macrovesicular
T3346 10241-10248 JJ denotes hepatic
T3342 10249-10258 NN denotes steatosis
T3347 10259-10261 IN denotes in
T3348 10262-10263 CD denotes 6
T3350 10263-10264 SYM denotes –
T3349 10264-10265 CD denotes 8
T3352 10265-10266 HYPH denotes -
T3351 10266-10268 NN denotes wk
T3354 10268-10269 HYPH denotes -
T3353 10269-10272 JJ denotes old
T3356 10273-10277 NN denotes MCAD
T3357 10277-10278 SYM denotes −
T3358 10278-10279 HYPH denotes /
T3359 10279-10280 SYM denotes −
T3355 10281-10285 NNS denotes mice
T3360 10286-10293 IN denotes whereas
T3362 10294-10298 NN denotes MCAD
T3364 10298-10299 SYM denotes +
T3365 10299-10300 HYPH denotes /
T3366 10300-10301 SYM denotes +
T3363 10302-10306 NNS denotes mice
T3361 10307-10310 VBD denotes had
T3367 10311-10313 DT denotes no
T3369 10314-10324 JJ denotes histologic
T3368 10325-10332 NNS denotes changes
T3370 10333-10334 -LRB- denotes (
T3372 10334-10340 NN denotes Figure
T3371 10341-10343 CD denotes 5A
T3373 10344-10347 CC denotes and
T3374 10348-10350 CD denotes 5B
T3375 10350-10351 -RRB- denotes )
T3376 10351-10352 . denotes .
T3377 10352-10487 sentence denotes In another group of 4-wk-old MCAD+/+ and MCAD−/− mice fasted for 24-h, there were minimal to no abnormalities in all organs evaluated.
T3378 10353-10355 IN denotes In
T3380 10356-10363 DT denotes another
T3381 10364-10369 NN denotes group
T3382 10370-10372 IN denotes of
T3383 10373-10374 CD denotes 4
T3385 10374-10375 HYPH denotes -
T3384 10375-10377 NN denotes wk
T3387 10377-10378 HYPH denotes -
T3386 10378-10381 JJ denotes old
T3389 10382-10386 NN denotes MCAD
T3390 10386-10387 SYM denotes +
T3391 10387-10388 HYPH denotes /
T3392 10388-10389 SYM denotes +
T3393 10390-10393 CC denotes and
T3394 10394-10398 NN denotes MCAD
T3395 10398-10399 SYM denotes −
T3396 10399-10400 HYPH denotes /
T3397 10400-10401 SYM denotes −
T3388 10402-10406 NNS denotes mice
T3398 10407-10413 VBN denotes fasted
T3399 10414-10417 IN denotes for
T3400 10418-10420 CD denotes 24
T3402 10420-10421 HYPH denotes -
T3401 10421-10422 NN denotes h
T3403 10422-10424 , denotes ,
T3404 10424-10429 EX denotes there
T3379 10430-10434 VBD denotes were
T3405 10435-10442 JJ denotes minimal
T3406 10443-10445 IN denotes to
T3407 10446-10448 DT denotes no
T3408 10449-10462 NNS denotes abnormalities
T3409 10463-10465 IN denotes in
T3410 10466-10469 DT denotes all
T3411 10470-10476 NNS denotes organs
T3412 10477-10486 VBN denotes evaluated
T3413 10486-10487 . denotes .
T3414 10487-10559 sentence denotes Only the older MCAD−/− mice, therefore, demonstrated hepatic steatosis.
T3415 10488-10492 RB denotes Only
T3417 10493-10496 DT denotes the
T3418 10497-10502 JJR denotes older
T3419 10503-10507 NN denotes MCAD
T3420 10507-10508 SYM denotes −
T3421 10508-10509 HYPH denotes /
T3422 10509-10510 SYM denotes −
T3416 10511-10515 NNS denotes mice
T3424 10515-10517 , denotes ,
T3425 10517-10526 RB denotes therefore
T3426 10526-10528 , denotes ,
T3423 10528-10540 VBD denotes demonstrated
T3427 10541-10548 JJ denotes hepatic
T3428 10549-10558 NN denotes steatosis
T3429 10558-10559 . denotes .
T3430 10559-10632 sentence denotes In addition, we found sporadic cardiac lesions in multiple MCAD−/− mice.
T3431 10560-10562 IN denotes In
T3433 10563-10571 NN denotes addition
T3434 10571-10573 , denotes ,
T3435 10573-10575 PRP denotes we
T3432 10576-10581 VBD denotes found
T3436 10582-10590 JJ denotes sporadic
T3438 10591-10598 JJ denotes cardiac
T3437 10599-10606 NNS denotes lesions
T3439 10607-10609 IN denotes in
T3440 10610-10618 JJ denotes multiple
T3442 10619-10623 NN denotes MCAD
T3443 10623-10624 SYM denotes −
T3444 10624-10625 HYPH denotes /
T3445 10625-10626 SYM denotes −
T3441 10627-10631 NNS denotes mice
T3446 10631-10632 . denotes .
T3447 10632-11248 sentence denotes Figure 5 Histopathology of MCAD+/+ and MCAD−/− Mice (A) MCAD+/+ mice had no evidence of hepatic steatosis following a 24-h fast. Liver section with Oil-Red O stain. (B) Hepatosteatosis in MCAD−/− mouse following a 24-h fast. Oil-Red O stained liver sections revealed severe and diffuse microvesicular and macrovesicular hepatic steatosis in MCAD−/− mice. (C and D) Diffuse cardiomyopathy with chronic active multifocal myocyte degeneration and necrosis in MCAD−/− mice. In one example, an MCAD−/− mouse had diffuse cardiomyopathy with chronic active multifocal myocyte degeneration and necrosis (Figure 5C and 5D).
T8009 10643-10657 NN denotes Histopathology
T8010 10658-10660 IN denotes of
T8011 10661-10665 NN denotes MCAD
T8013 10665-10666 SYM denotes +
T8014 10666-10667 HYPH denotes /
T8015 10667-10668 SYM denotes +
T8016 10669-10672 CC denotes and
T8017 10673-10677 NN denotes MCAD
T8018 10677-10678 SYM denotes −
T8019 10678-10679 HYPH denotes /
T8020 10679-10680 SYM denotes −
T8012 10681-10685 NNS denotes Mice
T8021 10685-10762 sentence denotes (A) MCAD+/+ mice had no evidence of hepatic steatosis following a 24-h fast.
T8022 10686-10687 -LRB- denotes (
T8023 10687-10688 LS denotes A
T8025 10688-10689 -RRB- denotes )
T8026 10690-10694 NN denotes MCAD
T8028 10694-10695 SYM denotes +
T8029 10695-10696 HYPH denotes /
T8030 10696-10697 SYM denotes +
T8027 10698-10702 NNS denotes mice
T8024 10703-10706 VBD denotes had
T8031 10707-10709 DT denotes no
T8032 10710-10718 NN denotes evidence
T8033 10719-10721 IN denotes of
T8034 10722-10729 JJ denotes hepatic
T8035 10730-10739 NN denotes steatosis
T8036 10740-10749 VBG denotes following
T8037 10750-10751 DT denotes a
T8039 10752-10754 CD denotes 24
T8041 10754-10755 HYPH denotes -
T8040 10755-10756 NN denotes h
T8038 10757-10761 NN denotes fast
T8042 10761-10762 . denotes .
T8043 10762-10798 sentence denotes Liver section with Oil-Red O stain.
T8044 10763-10768 NN denotes Liver
T8045 10769-10776 NN denotes section
T8046 10777-10781 IN denotes with
T8047 10782-10785 NN denotes Oil
T8049 10785-10786 HYPH denotes -
T8050 10786-10789 JJ denotes Red
T8048 10790-10791 NN denotes O
T8051 10792-10797 NN denotes stain
T8052 10797-10798 . denotes .
T8053 10798-10858 sentence denotes (B) Hepatosteatosis in MCAD−/− mouse following a 24-h fast.
T8054 10799-10800 -LRB- denotes (
T8055 10800-10801 LS denotes B
T8057 10801-10802 -RRB- denotes )
T8056 10803-10818 NN denotes Hepatosteatosis
T8058 10819-10821 IN denotes in
T8059 10822-10826 NN denotes MCAD
T8061 10826-10827 NN denotes −
T8062 10827-10828 HYPH denotes /
T8060 10828-10829 NN denotes −
T8063 10830-10835 NN denotes mouse
T8064 10836-10845 VBG denotes following
T8065 10846-10847 DT denotes a
T8067 10848-10850 CD denotes 24
T8069 10850-10851 HYPH denotes -
T8068 10851-10852 NN denotes h
T8066 10853-10857 NN denotes fast
T8070 10857-10858 . denotes .
T8071 10858-10988 sentence denotes Oil-Red O stained liver sections revealed severe and diffuse microvesicular and macrovesicular hepatic steatosis in MCAD−/− mice.
T8072 10859-10862 NN denotes Oil
T8074 10862-10863 HYPH denotes -
T8075 10863-10866 JJ denotes Red
T8073 10867-10868 NN denotes O
T8076 10869-10876 VBN denotes stained
T8078 10877-10882 NN denotes liver
T8077 10883-10891 NNS denotes sections
T8079 10892-10900 VBD denotes revealed
T8080 10901-10907 JJ denotes severe
T8082 10908-10911 CC denotes and
T8083 10912-10919 JJ denotes diffuse
T8084 10920-10934 JJ denotes microvesicular
T8085 10935-10938 CC denotes and
T8086 10939-10953 JJ denotes macrovesicular
T8087 10954-10961 JJ denotes hepatic
T8081 10962-10971 NN denotes steatosis
T8088 10972-10974 IN denotes in
T8089 10975-10979 NN denotes MCAD
T8091 10979-10980 SYM denotes −
T8092 10980-10981 HYPH denotes /
T8093 10981-10982 SYM denotes −
T8090 10983-10987 NNS denotes mice
T8094 10987-10988 . denotes .
T8095 10988-11103 sentence denotes (C and D) Diffuse cardiomyopathy with chronic active multifocal myocyte degeneration and necrosis in MCAD−/− mice.
T8096 10989-10990 -LRB- denotes (
T8097 10990-10991 LS denotes C
T8099 10992-10995 CC denotes and
T8100 10996-10997 LS denotes D
T8101 10997-10998 -RRB- denotes )
T8102 10999-11006 JJ denotes Diffuse
T8098 11007-11021 NN denotes cardiomyopathy
T8103 11022-11026 IN denotes with
T8104 11027-11034 JJ denotes chronic
T8106 11035-11041 JJ denotes active
T8107 11042-11052 JJ denotes multifocal
T8108 11053-11060 NN denotes myocyte
T8105 11061-11073 NN denotes degeneration
T8109 11074-11077 CC denotes and
T8110 11078-11086 NN denotes necrosis
T8111 11087-11089 IN denotes in
T8112 11090-11094 NN denotes MCAD
T8114 11094-11095 SYM denotes −
T8115 11095-11096 HYPH denotes /
T8116 11096-11097 SYM denotes −
T8113 11098-11102 NNS denotes mice
T8117 11102-11103 . denotes .
T3448 11104-11106 IN denotes In
T3450 11107-11110 CD denotes one
T3451 11111-11118 NN denotes example
T3452 11118-11120 , denotes ,
T3453 11120-11122 DT denotes an
T3455 11123-11127 NN denotes MCAD
T3456 11127-11128 SYM denotes −
T3457 11128-11129 HYPH denotes /
T3458 11129-11130 SYM denotes −
T3454 11131-11136 NN denotes mouse
T3449 11137-11140 VBD denotes had
T3459 11141-11148 JJ denotes diffuse
T3460 11149-11163 NN denotes cardiomyopathy
T3461 11164-11168 IN denotes with
T3462 11169-11176 JJ denotes chronic
T3464 11177-11183 JJ denotes active
T3465 11184-11194 JJ denotes multifocal
T3466 11195-11202 NN denotes myocyte
T3463 11203-11215 NN denotes degeneration
T3467 11216-11219 CC denotes and
T3468 11220-11228 NN denotes necrosis
T3469 11229-11230 -LRB- denotes (
T3471 11230-11236 NN denotes Figure
T3470 11237-11239 CD denotes 5C
T3472 11240-11243 CC denotes and
T3473 11244-11246 CD denotes 5D
T3474 11246-11247 -RRB- denotes )
T3475 11247-11248 . denotes .
T3476 11248-11421 sentence denotes Changes in degenerating myocytes included swelling, pale staining, and, in portions of the sarcoplasm, replacement of myofibrils with finely granular eosinophilic material.
T3477 11249-11256 NNS denotes Changes
T3479 11257-11259 IN denotes in
T3480 11260-11272 VBG denotes degenerating
T3481 11273-11281 NNS denotes myocytes
T3478 11282-11290 VBD denotes included
T3482 11291-11299 VBG denotes swelling
T3483 11299-11301 , denotes ,
T3484 11301-11305 JJ denotes pale
T3485 11306-11314 NN denotes staining
T3486 11314-11316 , denotes ,
T3487 11316-11319 CC denotes and
T3488 11319-11321 , denotes ,
T3489 11321-11323 IN denotes in
T3491 11324-11332 NNS denotes portions
T3492 11333-11335 IN denotes of
T3493 11336-11339 DT denotes the
T3494 11340-11350 NN denotes sarcoplasm
T3495 11350-11352 , denotes ,
T3490 11352-11363 NN denotes replacement
T3496 11364-11366 IN denotes of
T3497 11367-11377 NNS denotes myofibrils
T3498 11378-11382 IN denotes with
T3499 11383-11389 RB denotes finely
T3500 11390-11398 JJ denotes granular
T3502 11399-11411 JJ denotes eosinophilic
T3501 11412-11420 NN denotes material
T3503 11420-11421 . denotes .
T3504 11421-11509 sentence denotes Nuclei of affected myocytes were large, pale, and vesicular and had prominent nucleoli.
T3505 11422-11428 NNS denotes Nuclei
T3507 11429-11431 IN denotes of
T3508 11432-11440 VBN denotes affected
T3509 11441-11449 NNS denotes myocytes
T3506 11450-11454 VBD denotes were
T3510 11455-11460 JJ denotes large
T3511 11460-11462 , denotes ,
T3512 11462-11466 JJ denotes pale
T3513 11466-11468 , denotes ,
T3514 11468-11471 CC denotes and
T3515 11472-11481 JJ denotes vesicular
T3516 11482-11485 CC denotes and
T3517 11486-11489 VBD denotes had
T3518 11490-11499 JJ denotes prominent
T3519 11500-11508 NNS denotes nucleoli
T3520 11508-11509 . denotes .
T3521 11509-11598 sentence denotes In the most severely affected areas, there was loss of myocytes accompanied by fibrosis.
T3522 11510-11512 IN denotes In
T3524 11513-11516 DT denotes the
T3526 11517-11521 RBS denotes most
T3527 11522-11530 RB denotes severely
T3528 11531-11539 VBN denotes affected
T3525 11540-11545 NNS denotes areas
T3529 11545-11547 , denotes ,
T3530 11547-11552 EX denotes there
T3523 11553-11556 VBD denotes was
T3531 11557-11561 NN denotes loss
T3532 11562-11564 IN denotes of
T3533 11565-11573 NNS denotes myocytes
T3534 11574-11585 VBN denotes accompanied
T3535 11586-11588 IN denotes by
T3536 11589-11597 NN denotes fibrosis
T3537 11597-11598 . denotes .
T3538 11598-11825 sentence denotes In the wall of the aorta at the base of the heart there was multifocal degeneration of the elastic tissue, accompanied by multifocal collections of globular translucent yellow-brown pigment interpreted to be ceroid/lipofuscin.
T3539 11599-11601 IN denotes In
T3541 11602-11605 DT denotes the
T3542 11606-11610 NN denotes wall
T3543 11611-11613 IN denotes of
T3544 11614-11617 DT denotes the
T3545 11618-11623 NN denotes aorta
T3546 11624-11626 IN denotes at
T3547 11627-11630 DT denotes the
T3548 11631-11635 NN denotes base
T3549 11636-11638 IN denotes of
T3550 11639-11642 DT denotes the
T3551 11643-11648 NN denotes heart
T3552 11649-11654 EX denotes there
T3540 11655-11658 VBD denotes was
T3553 11659-11669 JJ denotes multifocal
T3554 11670-11682 NN denotes degeneration
T3555 11683-11685 IN denotes of
T3556 11686-11689 DT denotes the
T3558 11690-11697 JJ denotes elastic
T3557 11698-11704 NN denotes tissue
T3559 11704-11706 , denotes ,
T3560 11706-11717 VBN denotes accompanied
T3561 11718-11720 IN denotes by
T3562 11721-11731 JJ denotes multifocal
T3563 11732-11743 NNS denotes collections
T3564 11744-11746 IN denotes of
T3565 11747-11755 JJ denotes globular
T3567 11756-11767 JJ denotes translucent
T3568 11768-11774 JJ denotes yellow
T3570 11774-11775 HYPH denotes -
T3569 11775-11780 JJ denotes brown
T3566 11781-11788 NN denotes pigment
T3571 11789-11800 VBN denotes interpreted
T3572 11801-11803 TO denotes to
T3573 11804-11806 VB denotes be
T3574 11807-11813 NN denotes ceroid
T3576 11813-11814 HYPH denotes /
T3575 11814-11824 NN denotes lipofuscin
T3577 11824-11825 . denotes .
T3578 11825-11889 sentence denotes Similar deposits were scattered within adjacent adipose tissue.
T3579 11826-11833 JJ denotes Similar
T3580 11834-11842 NNS denotes deposits
T3582 11843-11847 VBD denotes were
T3581 11848-11857 VBN denotes scattered
T3583 11858-11864 IN denotes within
T3584 11865-11873 JJ denotes adjacent
T3586 11874-11881 NN denotes adipose
T3585 11882-11888 NN denotes tissue
T3587 11888-11889 . denotes .
R1000 T1418 T1414 punct −,MCAD
R1001 T1419 T1413 auxpass were,ascertained
R1002 T1420 T1413 prep by,ascertained
R1003 T1421 T1422 compound immunoblot,analysis
R1004 T1422 T1420 pobj analysis,by
R1005 T1423 T1422 prep of,analysis
R1006 T1424 T1423 pobj offspring,of
R1007 T1425 T1422 prep with,analysis
R1008 T1426 T1427 amod subsequent,perpetuation
R1009 T1427 T1425 pobj perpetuation,with
R1010 T1428 T1427 prep of,perpetuation
R1011 T1429 T1430 nmod MCAD,mice
R1012 T1430 T1428 pobj mice,of
R1013 T1431 T1429 punct −,MCAD
R1014 T1432 T1429 punct /,MCAD
R1015 T1433 T1429 punct −,MCAD
R1016 T1434 T1429 cc and,MCAD
R1017 T1435 T1429 conj MCAD,MCAD
R1018 T1436 T1435 punct +,MCAD
R1019 T1437 T1435 punct /,MCAD
R1020 T1438 T1435 punct +,MCAD
R1021 T1439 T1427 prep as,perpetuation
R1022 T1440 T1441 amod separate,groups
R1023 T1441 T1439 pobj groups,as
R1024 T1442 T1413 punct .,ascertained
R1025 T1606 T1607 compound RNA,Analysis
R1026 T1609 T1610 compound RT,PCR
R1027 T1610 T1612 compound PCR,amplification
R1028 T1611 T1610 punct -,PCR
R1029 T1612 T1613 nsubj amplification,amplified
R1030 T1614 T1612 prep from,amplification
R1031 T1615 T1616 nmod exon,7
R1032 T1616 T1614 pobj 7,from
R1033 T1617 T1616 prep to,7
R1034 T1618 T1617 pobj 11,to
R1035 T1619 T1612 prep from,amplification
R1036 T1620 T1621 amod total,RNA
R1037 T1621 T1619 pobj RNA,from
R1038 T1622 T1621 compound heart,RNA
R1039 T1623 T1624 det the,fragment
R1040 T1624 T1613 dobj fragment,amplified
R1041 T1625 T1624 amod expected,fragment
R1042 T1626 T1627 nummod 600,pair
R1043 T1627 T1624 nmod pair,fragment
R1044 T1628 T1627 punct -,pair
R1045 T1629 T1627 nmod base,pair
R1046 T1630 T1627 punct (,pair
R1047 T1631 T1627 appos bp,pair
R1048 T1632 T1624 punct ),fragment
R1049 T1633 T1624 prep in,fragment
R1050 T1634 T1635 nmod MCAD,mice
R1051 T1635 T1633 pobj mice,in
R1052 T1636 T1634 punct +,MCAD
R1053 T1637 T1634 punct /,MCAD
R1054 T1638 T1634 punct +,MCAD
R1055 T1639 T1634 cc and,MCAD
R1056 T1640 T1634 conj MCAD,MCAD
R1057 T1641 T1640 punct +,MCAD
R1058 T1642 T1640 punct /,MCAD
R1059 T1643 T1640 punct −,MCAD
R1060 T1644 T1624 punct ", ",fragment
R1061 T1645 T1624 cc and,fragment
R1062 T1646 T1647 det a,fragment
R1063 T1647 T1624 conj fragment,fragment
R1064 T1648 T1649 nummod 1.5,kb
R1065 T1649 T1647 compound kb,fragment
R1066 T1650 T1649 punct -,kb
R1067 T1651 T1647 prep in,fragment
R1068 T1652 T1653 nmod MCAD,mice
R1069 T1653 T1651 pobj mice,in
R1070 T1654 T1652 punct −,MCAD
R1071 T1655 T1652 punct /,MCAD
R1072 T1656 T1652 punct −,MCAD
R1073 T1657 T1658 punct (,shown
R1074 T1658 T1613 parataxis shown,amplified
R1075 T1659 T1658 nsubj data,shown
R1076 T1660 T1658 neg not,shown
R1077 T1661 T1658 punct ),shown
R1078 T1662 T1613 punct .,amplified
R1079 T1664 T1665 compound Sequence,analysis
R1080 T1665 T1666 nsubj analysis,revealed
R1081 T1667 T1665 prep of,analysis
R1082 T1668 T1669 det the,fragment
R1083 T1669 T1667 pobj fragment,of
R1084 T1670 T1671 nummod 1.5,kb
R1085 T1671 T1669 compound kb,fragment
R1086 T1672 T1671 punct -,kb
R1087 T1673 T1674 mark that,consisted
R1088 T1674 T1666 ccomp consisted,revealed
R1089 T1675 T1676 det the,fragment
R1090 T1676 T1674 nsubj fragment,consisted
R1091 T1677 T1676 amod amplified,fragment
R1092 T1678 T1674 prep of,consisted
R1093 T1679 T1678 pobj exon,of
R1094 T1680 T1679 nummod 7,exon
R1095 T1681 T1679 prep to,exon
R1096 T1682 T1681 pobj exon,to
R1097 T1683 T1682 nummod 10,exon
R1098 T1684 T1674 prep with,consisted
R1099 T1685 T1686 nummod 280,bp
R1100 T1686 T1684 pobj bp,with
R1101 T1687 T1686 prep of,bp
R1102 T1688 T1689 compound pGEM,sequence
R1103 T1689 T1687 pobj sequence,of
R1104 T1690 T1689 compound plasmid,sequence
R1105 T1691 T1689 acl followed,sequence
R1106 T1692 T1691 agent by,followed
R1107 T1693 T1694 nmod exons,8
R1108 T1694 T1692 pobj 8,by
R1109 T1695 T1696 punct –,11
R1110 T1696 T1694 prep 11,8
R1111 T1697 T1666 punct .,revealed
R1112 T1699 T1700 nsubjpass Some,deleted
R1113 T1701 T1699 prep of,Some
R1114 T1702 T1703 det the,sequences
R1115 T1703 T1701 pobj sequences,of
R1116 T1704 T1703 compound plasmid,sequences
R1117 T1705 T1699 punct ", ",Some
R1118 T1706 T1699 cc along,Some
R1119 T1707 T1706 dep with,along
R1120 T1708 T1709 det the,sequence
R1121 T1709 T1699 conj sequence,Some
R1122 T1710 T1709 compound pPGKNEOpA,sequence
R1123 T1711 T1700 punct ", ",deleted
R1124 T1712 T1700 auxpass were,deleted
R1125 T1713 T1700 prep from,deleted
R1126 T1714 T1715 det this,mRNA
R1127 T1715 T1713 pobj mRNA,from
R1128 T1716 T1715 amod spliced,mRNA
R1129 T1717 T1700 punct .,deleted
R1130 T1719 T1720 compound Northern,blot
R1131 T1720 T1721 compound blot,analysis
R1132 T1721 T1722 nsubj analysis,revealed
R1133 T1723 T1724 nsubjpass Acadm,expressed
R1134 T1724 T1722 advcl expressed,revealed
R1135 T1725 T1724 auxpass was,expressed
R1136 T1726 T1724 advmod normally,expressed
R1137 T1727 T1724 prep in,expressed
R1138 T1728 T1729 det all,tissues
R1139 T1729 T1727 pobj tissues,in
R1140 T1730 T1729 acl analyzed,tissues
R1141 T1731 T1729 prep from,tissues
R1142 T1732 T1733 nmod MCAD,mice
R1143 T1733 T1731 pobj mice,from
R1144 T1734 T1732 punct +,MCAD
R1145 T1735 T1732 punct /,MCAD
R1146 T1736 T1732 punct +,MCAD
R1147 T1737 T1724 prep with,expressed
R1148 T1738 T1739 det the,expression
R1149 T1739 T1742 nsubj expression,occurring
R1150 T1740 T1741 advmod most,robust
R1151 T1741 T1739 amod robust,expression
R1152 T1742 T1737 pcomp occurring,with
R1153 T1743 T1742 prep in,occurring
R1154 T1744 T1745 amod brown,fat
R1155 T1745 T1743 pobj fat,in
R1156 T1746 T1745 punct ", ",fat
R1157 T1747 T1745 conj kidney,fat
R1158 T1748 T1747 punct ", ",kidney
R1159 T1749 T1747 conj heart,kidney
R1160 T1750 T1749 punct ", ",heart
R1161 T1751 T1752 amod skeletal,muscle
R1162 T1752 T1749 conj muscle,heart
R1163 T1753 T1752 punct ", ",muscle
R1164 T1754 T1752 cc and,muscle
R1165 T1755 T1752 conj liver,muscle
R1166 T1756 T1724 prep with,expressed
R1167 T1757 T1758 amod minimal,expression
R1168 T1758 T1756 pobj expression,with
R1169 T1759 T1758 prep in,expression
R1170 T1760 T1761 det the,brain
R1171 T1761 T1759 pobj brain,in
R1172 T1762 T1761 punct ", ",brain
R1173 T1763 T1764 amod white,fat
R1174 T1764 T1761 conj fat,brain
R1175 T1765 T1764 punct ", ",fat
R1176 T1766 T1764 cc and,fat
R1177 T1767 T1764 conj testes,fat
R1178 T1768 T1769 punct (,Figure
R1179 T1769 T1722 parataxis Figure,revealed
R1180 T1770 T1769 nummod 2,Figure
R1181 T1771 T1769 punct ),Figure
R1182 T1772 T1722 punct .,revealed
R1183 T1774 T1775 advmod Interestingly,detected
R1184 T1776 T1775 punct ", ",detected
R1185 T1777 T1778 mark although,amplified
R1186 T1778 T1775 advcl amplified,detected
R1187 T1779 T1780 compound RT,PCR
R1188 T1780 T1778 nsubj PCR,amplified
R1189 T1781 T1780 punct -,PCR
R1190 T1782 T1783 det an,transcript
R1191 T1783 T1778 dobj transcript,amplified
R1192 T1784 T1785 advmod incorrectly,spliced
R1193 T1785 T1783 amod spliced,transcript
R1194 T1786 T1783 compound Acadm,transcript
R1195 T1787 T1775 punct ", ",detected
R1196 T1788 T1789 det no,transcripts
R1197 T1789 T1775 nsubjpass transcripts,detected
R1198 T1790 T1789 compound Acadm,transcripts
R1199 T1791 T1775 auxpass were,detected
R1200 T1792 T1775 prep by,detected
R1201 T1793 T1794 compound northern,blot
R1202 T1794 T1795 compound blot,analysis
R1203 T1795 T1792 pobj analysis,by
R1204 T1796 T1775 prep from,detected
R1205 T1797 T1798 nmod MCAD,mice
R1206 T1798 T1796 pobj mice,from
R1207 T1799 T1797 punct −,MCAD
R1208 T1800 T1797 punct /,MCAD
R1209 T1801 T1797 punct −,MCAD
R1210 T1802 T1775 punct .,detected
R1211 T1804 T1805 det These,results
R1212 T1805 T1806 nsubj results,suggest
R1213 T1807 T1806 advmod strongly,suggest
R1214 T1808 T1809 mark that,is
R1215 T1809 T1806 ccomp is,suggest
R1216 T1810 T1811 det the,RNA
R1217 T1811 T1809 nsubj RNA,is
R1218 T1812 T1811 compound mutant,RNA
R1219 T1813 T1809 acomp unstable,is
R1220 T1814 T1809 cc and,is
R1221 T1815 T1809 conj degraded,is
R1222 T1816 T1815 advmod rapidly,degraded
R1223 T1817 T1809 cc or,is
R1224 T1818 T1809 punct ", ",is
R1225 T1819 T1820 advmod alternatively,undergoes
R1226 T1820 T1809 conj undergoes,is
R1227 T1821 T1820 punct ", ",undergoes
R1228 T1822 T1823 npadvmod nonsense,mediated
R1229 T1823 T1824 amod mediated,decay
R1230 T1824 T1820 dobj decay,undergoes
R1231 T1825 T1824 compound RNA,decay
R1232 T1826 T1806 punct .,suggest
R1235 T1979 T1980 compound Liver,Enzyme
R1236 T1980 T1981 compound Enzyme,Analyses
R1237 T1983 T1984 compound Immunoblot,analyses
R1238 T1984 T1985 nsubj analyses,demonstrated
R1239 T1986 T1984 prep of,analyses
R1240 T1987 T1988 compound liver,homogenates
R1241 T1988 T1986 pobj homogenates,of
R1242 T1989 T1984 prep with,analyses
R1243 T1990 T1991 amod anti-MCAD,antisera
R1244 T1991 T1989 pobj antisera,with
R1245 T1992 T1993 mark that,was
R1246 T1993 T1985 ccomp was,demonstrated
R1247 T1994 T1995 det the,monomer
R1248 T1995 T1993 nsubj monomer,was
R1249 T1996 T1997 nummod 42,kDa
R1250 T1997 T1995 compound kDa,monomer
R1251 T1998 T1995 compound MCAD,monomer
R1252 T1999 T1993 acomp present,was
R1253 T2000 T1993 prep in,was
R1254 T2001 T2002 nmod MCAD,mice
R1255 T2002 T2000 pobj mice,in
R1256 T2003 T2001 punct +,MCAD
R1257 T2004 T2001 punct /,MCAD
R1258 T2005 T2001 punct +,MCAD
R1259 T2006 T2000 punct ", ",in
R1260 T2007 T2000 cc but,in
R1261 T2008 T2007 neg not,but
R1262 T2009 T2000 conj in,in
R1263 T2010 T2011 nmod MCAD,mice
R1264 T2011 T2009 pobj mice,in
R1265 T2012 T2010 punct −,MCAD
R1266 T2013 T2010 punct /,MCAD
R1267 T2014 T2010 punct −,MCAD
R1268 T2015 T2016 punct (,Figure
R1269 T2016 T1985 parataxis Figure,demonstrated
R1270 T2017 T2016 nummod 3,Figure
R1271 T2018 T2016 punct ),Figure
R1272 T2019 T1985 punct .,demonstrated
R1273 T2021 T2022 prep As,revealed
R1274 T2023 T2024 det a,analysis
R1275 T2024 T2021 pobj analysis,As
R1276 T2025 T2024 compound control,analysis
R1277 T2026 T2022 punct ", ",revealed
R1278 T2027 T2028 amod anti–short,chain
R1279 T2028 T2030 nmod chain,dehydrogenase
R1280 T2029 T2028 punct -,chain
R1281 T2030 T2034 nmod dehydrogenase,SCAD
R1282 T2031 T2032 nmod acyl,CoA
R1283 T2032 T2030 nmod CoA,dehydrogenase
R1284 T2033 T2032 punct -,CoA
R1285 T2034 T2036 nmod SCAD,antisera
R1286 T2035 T2034 punct (,SCAD
R1287 T2036 T2022 nsubj antisera,revealed
R1288 T2037 T2034 punct ),SCAD
R1289 T2038 T2039 det no,differences
R1290 T2039 T2022 dobj differences,revealed
R1291 T2040 T2039 prep in,differences
R1292 T2041 T2040 pobj levels,in
R1293 T2042 T2041 prep of,levels
R1294 T2043 T2042 pobj expression,of
R1295 T2044 T2043 prep of,expression
R1296 T2045 T2046 compound SCAD,protein
R1297 T2046 T2044 pobj protein,of
R1298 T2047 T2039 prep between,differences
R1299 T2048 T2049 nmod MCAD,mice
R1300 T2049 T2047 pobj mice,between
R1301 T2050 T2048 punct +,MCAD
R1302 T2051 T2048 punct /,MCAD
R1303 T2052 T2048 punct +,MCAD
R1304 T2053 T2048 cc and,MCAD
R1305 T2054 T2048 conj MCAD,MCAD
R1306 T2055 T2054 punct −,MCAD
R1307 T2056 T2054 punct /,MCAD
R1308 T2057 T2054 punct −,MCAD
R1309 T2058 T2059 punct (,Figure
R1310 T2059 T2022 parataxis Figure,revealed
R1311 T2060 T2059 nummod 3,Figure
R1312 T2061 T2059 punct ),Figure
R1313 T2062 T2022 punct .,revealed
R1314 T2064 T2065 compound Enzyme,activity
R1315 T2065 T2066 nsubjpass activity,analyzed
R1316 T2067 T2066 auxpass was,analyzed
R1317 T2068 T2066 prep in,analyzed
R1318 T2069 T2070 compound mouse,homogenates
R1319 T2070 T2068 pobj homogenates,in
R1320 T2071 T2070 compound liver,homogenates
R1321 T2072 T2066 advcl using,analyzed
R1322 T2073 T2074 det the,assay
R1323 T2074 T2072 dobj assay,using
R1324 T2075 T2076 nmod electron,flavoprotein
R1325 T2076 T2074 nmod flavoprotein,assay
R1326 T2077 T2076 nmod transport,flavoprotein
R1327 T2078 T2076 punct (,flavoprotein
R1328 T2079 T2076 appos ETF,flavoprotein
R1329 T2080 T2074 punct ),assay
R1330 T2081 T2074 compound reduction,assay
R1331 T2082 T2072 prep with,using
R1332 T2083 T2084 compound octanoyl,CoA
R1333 T2084 T2082 pobj CoA,with
R1334 T2085 T2084 punct -,CoA
R1335 T2086 T2087 punct (,C8
R1336 T2087 T2084 parataxis C8,CoA
R1337 T2088 T2087 punct :,C8
R1338 T2089 T2087 nummod 0,C8
R1339 T2090 T2087 punct ),C8
R1340 T2091 T2084 cc and,CoA
R1341 T2092 T2093 compound palmitoyl,CoA
R1342 T2093 T2084 conj CoA,CoA
R1343 T2094 T2093 punct -,CoA
R1344 T2095 T2096 punct (,C16
R1345 T2096 T2093 parataxis C16,CoA
R1346 T2097 T2096 punct :,C16
R1347 T2098 T2096 nummod 0,C16
R1348 T2099 T2096 punct ),C16
R1349 T2100 T2084 prep as,CoA
R1350 T2101 T2100 pobj substrates,as
R1351 T2102 T2066 punct .,analyzed
R1352 T2104 T2105 nmod MCAD,mice
R1353 T2105 T2109 nsubj mice,had
R1354 T2106 T2104 punct −,MCAD
R1355 T2107 T2104 punct /,MCAD
R1356 T2108 T2104 punct −,MCAD
R1357 T2110 T2111 det a,reduction
R1358 T2111 T2109 dobj reduction,had
R1359 T2112 T2111 amod significant,reduction
R1360 T2113 T2111 prep in,reduction
R1361 T2114 T2113 pobj ability,in
R1362 T2115 T2116 aux to,dehydrogenate
R1363 T2116 T2114 acl dehydrogenate,ability
R1364 T2117 T2118 compound octanoyl,CoA
R1365 T2118 T2116 dobj CoA,dehydrogenate
R1366 T2119 T2118 punct -,CoA
R1367 T2120 T2111 cc and,reduction
R1368 T2121 T2122 det a,reduction
R1369 T2122 T2111 conj reduction,reduction
R1370 T2123 T2122 amod modest,reduction
R1371 T2124 T2122 prep in,reduction
R1372 T2125 T2124 pobj activity,in
R1373 T2126 T2125 prep toward,activity
R1374 T2127 T2128 compound palmitoyl,CoA
R1375 T2128 T2126 pobj CoA,toward
R1376 T2129 T2128 punct -,CoA
R1377 T2130 T2109 prep compared,had
R1378 T2131 T2130 prep to,compared
R1379 T2132 T2133 nmod MCAD,mice
R1380 T2133 T2131 pobj mice,to
R1381 T2134 T2132 punct +,MCAD
R1382 T2135 T2132 punct /,MCAD
R1383 T2136 T2132 punct +,MCAD
R1384 T2137 T2138 punct (,Table
R1385 T2138 T2109 parataxis Table,had
R1386 T2139 T2138 nummod 1,Table
R1387 T2140 T2138 punct ),Table
R1388 T2141 T2109 punct .,had
R1389 T2143 T2144 advmod Specifically,reduced
R1390 T2145 T2144 punct ", ",reduced
R1391 T2146 T2147 det the,dehydrogenation
R1392 T2147 T2144 nsubjpass dehydrogenation,reduced
R1393 T2148 T2147 prep of,dehydrogenation
R1394 T2149 T2150 nmod octanoyl,CoA
R1395 T2150 T2152 nmod CoA,substrates
R1396 T2151 T2150 punct -,CoA
R1397 T2152 T2148 pobj substrates,of
R1398 T2153 T2150 cc and,CoA
R1399 T2154 T2155 compound palmitoyl,CoA
R1400 T2155 T2150 conj CoA,CoA
R1401 T2156 T2155 punct -,CoA
R1402 T2157 T2144 auxpass were,reduced
R1403 T2158 T2144 prep by,reduced
R1404 T2159 T2160 nummod 75,%
R1405 T2160 T2158 pobj %,by
R1406 T2161 T2158 cc and,by
R1407 T2162 T2158 conj by,by
R1408 T2163 T2164 nummod 30,%
R1409 T2164 T2162 pobj %,by
R1410 T2165 T2144 punct ", ",reduced
R1411 T2166 T2144 advmod respectively,reduced
R1412 T2167 T2144 punct ", ",reduced
R1413 T2168 T2144 prep in,reduced
R1414 T2169 T2170 nmod MCAD,mice
R1415 T2170 T2168 pobj mice,in
R1416 T2171 T2169 punct −,MCAD
R1417 T2172 T2169 punct /,MCAD
R1418 T2173 T2169 punct −,MCAD
R1419 T2174 T2175 mark as,compared
R1420 T2175 T2144 advcl compared,reduced
R1421 T2176 T2175 prep to,compared
R1422 T2177 T2178 nmod MCAD,controls
R1423 T2178 T2176 pobj controls,to
R1424 T2179 T2177 punct +,MCAD
R1425 T2180 T2177 punct /,MCAD
R1426 T2181 T2177 punct +,MCAD
R1427 T2182 T2144 punct .,reduced
R1428 T2294 T2295 amod Neonatal,Deaths
R1429 T2297 T2298 amod Significant,mortality
R1430 T2298 T2300 nsubjpass mortality,noted
R1431 T2299 T2298 amod neonatal,mortality
R1432 T2301 T2300 auxpass was,noted
R1433 T2302 T2300 prep in,noted
R1434 T2303 T2304 nmod MCAD,pups
R1435 T2304 T2302 pobj pups,in
R1436 T2305 T2303 punct −,MCAD
R1437 T2306 T2303 punct /,MCAD
R1438 T2307 T2303 punct −,MCAD
R1439 T2308 T2300 punct .,noted
R1440 T2310 T2311 mark Although,born
R1441 T2311 T2317 advcl born,survived
R1442 T2312 T2313 amod equal,numbers
R1443 T2313 T2311 nsubjpass numbers,born
R1444 T2314 T2313 prep of,numbers
R1445 T2315 T2314 pobj pups,of
R1446 T2316 T2311 auxpass were,born
R1447 T2318 T2311 prep from,born
R1448 T2319 T2320 nmod MCAD,mice
R1449 T2320 T2318 pobj mice,from
R1450 T2321 T2319 punct +,MCAD
R1451 T2322 T2319 punct /,MCAD
R1452 T2323 T2319 punct +,MCAD
R1453 T2324 T2319 cc and,MCAD
R1454 T2325 T2319 conj MCAD,MCAD
R1455 T2326 T2325 punct −,MCAD
R1456 T2327 T2325 punct /,MCAD
R1457 T2328 T2325 punct −,MCAD
R1458 T2329 T2317 punct ", ",survived
R1459 T2330 T2331 advmod only,%
R1460 T2331 T2317 nsubj %,survived
R1461 T2332 T2331 nummod 41,%
R1462 T2333 T2331 prep of,%
R1463 T2334 T2335 nmod MCAD,mice
R1464 T2335 T2333 pobj mice,of
R1465 T2336 T2334 punct −,MCAD
R1466 T2337 T2334 punct /,MCAD
R1467 T2338 T2334 punct −,MCAD
R1468 T2339 T2317 prep to,survived
R1469 T2340 T2339 pobj weaning,to
R1470 T2341 T2342 mark as,compared
R1471 T2342 T2317 advcl compared,survived
R1472 T2343 T2342 prep to,compared
R1473 T2344 T2345 nummod 98,%
R1474 T2345 T2343 pobj %,to
R1475 T2346 T2345 prep of,%
R1476 T2347 T2348 nmod MCAD,mice
R1477 T2348 T2346 pobj mice,of
R1478 T2349 T2347 punct +,MCAD
R1479 T2350 T2347 punct /,MCAD
R1480 T2351 T2347 punct +,MCAD
R1481 T2352 T2353 punct (,Table
R1482 T2353 T2317 parataxis Table,survived
R1483 T2354 T2353 nummod 1,Table
R1484 T2355 T2353 punct ),Table
R1485 T2356 T2317 punct .,survived
R1486 T2358 T2359 det The,mechanism
R1487 T2359 T2360 nsubj mechanism,remains
R1488 T2361 T2359 prep of,mechanism
R1489 T2362 T2363 amod neonatal,loss
R1490 T2363 T2361 pobj loss,of
R1491 T2364 T2360 acomp undetermined,remains
R1492 T2365 T2360 punct .,remains
R1493 T2367 T2368 det The,pups
R1494 T2368 T2373 nsubjpass pups,abandoned
R1495 T2369 T2368 nmod MCAD,pups
R1496 T2370 T2369 punct −,MCAD
R1497 T2371 T2369 punct /,MCAD
R1498 T2372 T2369 punct −,MCAD
R1499 T2374 T2373 auxpass are,abandoned
R1500 T2375 T2376 advmod more,frequently
R1501 T2376 T2373 advmod frequently,abandoned
R1502 T2377 T2376 prep than,frequently
R1503 T2378 T2379 nmod MCAD,pups
R1504 T2379 T2377 pobj pups,than
R1505 T2380 T2378 punct +,MCAD
R1506 T2381 T2378 punct /,MCAD
R1507 T2382 T2378 punct +,MCAD
R1508 T2383 T2373 prep for,abandoned
R1509 T2384 T2385 amod unknown,reasons
R1510 T2385 T2383 pobj reasons,for
R1511 T2386 T2373 punct .,abandoned
R1512 T2388 T2389 nsubj They,are
R1513 T2390 T2389 advmod likely,are
R1514 T2391 T2392 advmod more,prone
R1515 T2392 T2389 acomp prone,are
R1516 T2393 T2392 prep to,prone
R1517 T2394 T2393 pobj hypothermia,to
R1518 T2395 T2392 prep than,prone
R1519 T2396 T2395 pobj MCAD,than
R1520 T2397 T2396 punct +,MCAD
R1521 T2398 T2396 punct /,MCAD
R1522 T2399 T2396 punct +,MCAD
R1523 T2400 T2389 punct .,are
R1524 T2402 T2403 prep Because,compare
R1525 T2404 T2402 pcomp of,Because
R1526 T2405 T2406 det the,difficulty
R1527 T2406 T2402 pobj difficulty,Because
R1528 T2407 T2406 prep of,difficulty
R1529 T2408 T2407 pcomp distinguishing,of
R1530 T2409 T2410 det the,mutants
R1531 T2410 T2408 dobj mutants,distinguishing
R1532 T2411 T2410 nmod MCAD,mutants
R1533 T2412 T2411 punct −,MCAD
R1534 T2413 T2411 punct /,MCAD
R1535 T2414 T2411 punct −,MCAD
R1536 T2415 T2408 prep from,distinguishing
R1537 T2416 T2417 det the,pups
R1538 T2417 T2415 pobj pups,from
R1539 T2418 T2417 nmod MCAD,pups
R1540 T2419 T2418 punct +,MCAD
R1541 T2420 T2418 punct /,MCAD
R1542 T2421 T2418 punct −,MCAD
R1543 T2422 T2417 amod heterozygous,pups
R1544 T2423 T2408 prep by,distinguishing
R1545 T2424 T2425 amod molecular,analysis
R1546 T2425 T2423 pobj analysis,by
R1547 T2426 T2406 prep due,difficulty
R1548 T2427 T2426 pcomp to,due
R1549 T2428 T2429 det the,mutation
R1550 T2429 T2426 pobj mutation,due
R1551 T2430 T2429 compound insertion,mutation
R1552 T2431 T2403 punct ", ",compare
R1553 T2432 T2403 nsubj we,compare
R1554 T2433 T2403 aux could,compare
R1555 T2434 T2403 advmod only,compare
R1556 T2435 T2436 nmod MCAD,matings
R1557 T2436 T2403 dobj matings,compare
R1558 T2437 T2435 punct +,MCAD
R1559 T2438 T2435 punct /,MCAD
R1560 T2439 T2435 punct +,MCAD
R1561 T2440 T2435 punct ×,MCAD
R1562 T2441 T2435 appos MCAD,MCAD
R1563 T2442 T2441 punct +,MCAD
R1564 T2443 T2441 punct /,MCAD
R1565 T2444 T2441 punct +,MCAD
R1566 T2445 T2403 prep with,compare
R1567 T2446 T2447 nmod MCAD,matings
R1568 T2447 T2445 pobj matings,with
R1569 T2448 T2446 punct −,MCAD
R1570 T2449 T2446 punct /,MCAD
R1571 T2450 T2446 punct −,MCAD
R1572 T2451 T2446 punct ×,MCAD
R1573 T2452 T2446 appos MCAD,MCAD
R1574 T2453 T2452 punct −,MCAD
R1575 T2454 T2452 punct /,MCAD
R1576 T2455 T2452 punct −,MCAD
R1577 T2456 T2403 punct .,compare
R1578 T2458 T2459 advmod Thus,were
R1579 T2460 T2459 punct ", ",were
R1580 T2461 T2459 nsubj we,were
R1581 T2462 T2459 acomp unable,were
R1582 T2463 T2464 aux to,evaluate
R1583 T2464 T2462 xcomp evaluate,unable
R1584 T2465 T2466 det the,pedigrees
R1585 T2466 T2464 dobj pedigrees,evaluate
R1586 T2467 T2464 prep from,evaluate
R1587 T2468 T2469 amod heterozygous,matings
R1588 T2469 T2467 pobj matings,from
R1589 T2470 T2459 punct .,were
R1590 T2566 T2565 cc and,Fasting
R1591 T2567 T2568 compound Cold,Intolerance
R1592 T2568 T2565 conj Intolerance,Fasting
R1593 T2570 T2571 prep In,fasted
R1594 T2572 T2570 pobj order,In
R1595 T2573 T2574 aux to,examine
R1596 T2574 T2572 acl examine,order
R1597 T2575 T2576 det the,effects
R1598 T2576 T2574 dobj effects,examine
R1599 T2577 T2576 compound stress,effects
R1600 T2578 T2576 prep of,effects
R1601 T2579 T2578 pobj fasting,of
R1602 T2580 T2576 prep on,effects
R1603 T2581 T2582 npadvmod MCAD,deficient
R1604 T2582 T2584 amod deficient,mice
R1605 T2583 T2582 punct -,deficient
R1606 T2584 T2580 pobj mice,on
R1607 T2585 T2571 punct ", ",fasted
R1608 T2586 T2571 nsubjpass they,fasted
R1609 T2587 T2571 auxpass were,fasted
R1610 T2588 T2571 prep for,fasted
R1611 T2589 T2590 nummod 24,h
R1612 T2590 T2588 pobj h,for
R1613 T2591 T2592 amod prior,to
R1614 T2592 T2571 prep to,fasted
R1615 T2593 T2592 pobj analysis,to
R1616 T2594 T2571 punct .,fasted
R1617 T2596 T2597 nmod MCAD,mice
R1618 T2597 T2601 nsubj mice,displayed
R1619 T2598 T2596 punct −,MCAD
R1620 T2599 T2596 punct /,MCAD
R1621 T2600 T2596 punct −,MCAD
R1622 T2602 T2603 amod lower,glucose
R1623 T2603 T2601 dobj glucose,displayed
R1624 T2604 T2603 compound serum,glucose
R1625 T2605 T2603 cc and,glucose
R1626 T2606 T2607 amod elevated,levels
R1627 T2607 T2603 conj levels,glucose
R1628 T2608 T2609 npadvmod serum,free
R1629 T2609 T2610 amod free,acid
R1630 T2610 T2607 compound acid,levels
R1631 T2611 T2610 amod fatty,acid
R1632 T2612 T2613 mark although,was
R1633 T2613 T2601 advcl was,displayed
R1634 T2614 T2615 det neither,result
R1635 T2615 T2613 nsubj result,was
R1636 T2616 T2613 acomp significant,was
R1637 T2617 T2613 punct ", ",was
R1638 T2618 T2619 mark as,compared
R1639 T2619 T2613 advcl compared,was
R1640 T2620 T2619 prep to,compared
R1641 T2621 T2622 nmod MCAD,mice
R1642 T2622 T2620 pobj mice,to
R1643 T2623 T2621 punct +,MCAD
R1644 T2624 T2621 punct /,MCAD
R1645 T2625 T2621 punct +,MCAD
R1646 T2626 T2627 punct (,Table
R1647 T2627 T2601 parataxis Table,displayed
R1648 T2628 T2627 nummod 1,Table
R1649 T2629 T2627 punct ),Table
R1650 T2630 T2601 punct .,displayed
R1651 T2632 T2633 aux To,determine
R1652 T2633 T2634 advcl determine,fasted
R1653 T2635 T2636 det the,effects
R1654 T2636 T2633 dobj effects,determine
R1655 T2637 T2636 prep of,effects
R1656 T2638 T2639 compound cold,stress
R1657 T2639 T2637 pobj stress,of
R1658 T2640 T2634 punct ", ",fasted
R1659 T2641 T2634 nsubjpass mice,fasted
R1660 T2642 T2634 auxpass were,fasted
R1661 T2643 T2634 prep for,fasted
R1662 T2644 T2645 nummod 18,h
R1663 T2645 T2643 pobj h,for
R1664 T2646 T2634 cc and,fasted
R1665 T2647 T2634 conj placed,fasted
R1666 T2648 T2647 prep in,placed
R1667 T2649 T2650 nummod 4,°C
R1668 T2650 T2651 compound °C,environment
R1669 T2651 T2648 pobj environment,in
R1670 T2652 T2647 prep for,placed
R1671 T2653 T2654 det a,period
R1672 T2654 T2652 pobj period,for
R1673 T2655 T2656 nummod 3,h
R1674 T2656 T2654 compound h,period
R1675 T2657 T2656 punct -,h
R1676 T2658 T2634 punct .,fasted
R1677 T2660 T2661 det The,mice
R1678 T2661 T2666 nsubjpass mice,compromised
R1679 T2662 T2661 nmod MCAD,mice
R1680 T2663 T2662 punct −,MCAD
R1681 T2664 T2662 punct /,MCAD
R1682 T2665 T2662 punct −,MCAD
R1683 T2667 T2666 auxpass were,compromised
R1684 T2668 T2666 advmod significantly,compromised
R1685 T2669 T2666 prep within,compromised
R1686 T2670 T2671 det this,period
R1687 T2671 T2669 pobj period,within
R1688 T2672 T2671 amod short,period
R1689 T2673 T2671 prep of,period
R1690 T2674 T2675 compound cold,stress
R1691 T2675 T2673 pobj stress,of
R1692 T2676 T2666 punct ", ",compromised
R1693 T2677 T2678 nsubj some,severe
R1694 T2678 T2666 amod severe,compromised
R1695 T2679 T2678 advmod enough,severe
R1696 T2680 T2681 aux to,result
R1697 T2681 T2678 xcomp result,severe
R1698 T2682 T2681 prep in,result
R1699 T2683 T2682 pobj fatalities,in
R1700 T2684 T2666 punct .,compromised
R1701 T2686 T2687 prep After,was
R1702 T2688 T2689 nummod 1,h
R1703 T2689 T2686 pobj h,After
R1704 T2690 T2689 prep of,h
R1705 T2691 T2692 det the,challenge
R1706 T2692 T2690 pobj challenge,of
R1707 T2693 T2692 compound cold,challenge
R1708 T2694 T2687 punct ", ",was
R1709 T2695 T2696 det the,temperature
R1710 T2696 T2687 nsubj temperature,was
R1711 T2697 T2696 amod average,temperature
R1712 T2698 T2696 amod rectal,temperature
R1713 T2699 T2696 prep of,temperature
R1714 T2700 T2701 nmod MCAD,mice
R1715 T2701 T2699 pobj mice,of
R1716 T2702 T2700 punct −,MCAD
R1717 T2703 T2700 punct /,MCAD
R1718 T2704 T2700 punct −,MCAD
R1719 T2705 T2706 punct (,5
R1720 T2706 T2701 parataxis 5,mice
R1721 T2707 T2706 nsubj n,5
R1722 T2708 T2706 punct =,5
R1723 T2709 T2706 punct ),5
R1724 T2710 T2711 nummod 23.4,°C
R1725 T2711 T2687 attr °C,was
R1726 T2712 T2713 mark as,compared
R1727 T2713 T2687 advcl compared,was
R1728 T2714 T2713 prep with,compared
R1729 T2715 T2716 nummod 35,°C
R1730 T2716 T2714 pobj °C,with
R1731 T2717 T2716 prep for,°C
R1732 T2718 T2719 nmod MCAD,mice
R1733 T2719 T2717 pobj mice,for
R1734 T2720 T2718 punct +,MCAD
R1735 T2721 T2718 punct /,MCAD
R1736 T2722 T2718 punct +,MCAD
R1737 T2723 T2724 punct (,4
R1738 T2724 T2719 parataxis 4,mice
R1739 T2725 T2724 nsubj n,4
R1740 T2726 T2724 punct =,4
R1741 T2727 T2724 punct ),4
R1742 T2728 T2687 punct .,was
R1743 T2730 T2731 amod Rectal,temperatures
R1744 T2731 T2732 nsubj temperatures,declined
R1745 T2733 T2732 prep to,declined
R1746 T2734 T2735 amod unrecoverable,temperatures
R1747 T2735 T2733 pobj temperatures,to
R1748 T2736 T2735 prep of,temperatures
R1749 T2737 T2738 quantmod 16.7,19.2
R1750 T2738 T2740 nummod 19.2,°C
R1751 T2739 T2738 punct –,19.2
R1752 T2740 T2736 pobj °C,of
R1753 T2741 T2732 prep in,declined
R1754 T2742 T2743 quantmod three,five
R1755 T2743 T2746 nummod five,mice
R1756 T2744 T2743 quantmod of,five
R1757 T2745 T2743 quantmod the,five
R1758 T2746 T2741 pobj mice,in
R1759 T2747 T2746 nmod MCAD,mice
R1760 T2748 T2747 punct −,MCAD
R1761 T2749 T2747 punct /,MCAD
R1762 T2750 T2747 punct −,MCAD
R1763 T2751 T2732 punct .,declined
R1764 T2753 T2754 prep By,averaged
R1765 T2755 T2756 det the,end
R1766 T2756 T2753 pobj end,By
R1767 T2757 T2756 prep of,end
R1768 T2758 T2759 det the,mark
R1769 T2759 T2757 pobj mark,of
R1770 T2760 T2761 nummod 1.5,h
R1771 T2761 T2759 compound h,mark
R1772 T2762 T2761 punct -,h
R1773 T2763 T2754 punct ", ",averaged
R1774 T2764 T2765 det the,mice
R1775 T2765 T2754 nsubj mice,averaged
R1776 T2766 T2765 nummod two,mice
R1777 T2767 T2765 amod surviving,mice
R1778 T2768 T2765 nmod MCAD,mice
R1779 T2769 T2768 punct −,MCAD
R1780 T2770 T2768 punct /,MCAD
R1781 T2771 T2768 punct −,MCAD
R1782 T2772 T2773 nummod 22.7,°C
R1783 T2773 T2754 dobj °C,averaged
R1784 T2774 T2754 punct .,averaged
R1785 T2776 T2777 prep In,survived
R1786 T2778 T2776 pobj contrast,In
R1787 T2779 T2777 punct ", ",survived
R1788 T2780 T2781 det all,mice
R1789 T2781 T2777 nsubj mice,survived
R1790 T2782 T2781 nummod four,mice
R1791 T2783 T2781 nmod MCAD,mice
R1792 T2784 T2783 punct +,MCAD
R1793 T2785 T2783 punct /,MCAD
R1794 T2786 T2783 punct +,MCAD
R1795 T2787 T2788 det the,stress
R1796 T2788 T2777 dobj stress,survived
R1797 T2789 T2790 nummod 3,h
R1798 T2790 T2788 compound h,stress
R1799 T2791 T2790 punct -,h
R1800 T2792 T2788 compound cold,stress
R1801 T2793 T2777 punct ", ",survived
R1802 T2794 T2777 advcl ending,survived
R1803 T2795 T2794 prep with,ending
R1804 T2796 T2797 det an,temperature
R1805 T2797 T2795 pobj temperature,with
R1806 T2798 T2797 amod average,temperature
R1807 T2799 T2797 amod rectal,temperature
R1808 T2800 T2797 prep of,temperature
R1809 T2801 T2802 nummod 33.6,°C
R1810 T2802 T2800 pobj °C,of
R1811 T2803 T2777 punct .,survived
R1812 T2908 T2909 amod Organic,Acid
R1813 T2910 T2909 cc and,Acid
R1814 T2911 T2912 compound Acylcarnitine,Analysis
R1815 T2912 T2909 conj Analysis,Acid
R1816 T2914 T2915 nmod Urine,acid
R1817 T2915 T2917 compound acid,analysis
R1818 T2916 T2915 amod organic,acid
R1819 T2917 T2918 nsubj analysis,revealed
R1820 T2919 T2920 mark that,developed
R1821 T2920 T2918 ccomp developed,revealed
R1822 T2921 T2922 nmod MCAD,mice
R1823 T2922 T2920 nsubj mice,developed
R1824 T2923 T2921 punct −,MCAD
R1825 T2924 T2921 punct /,MCAD
R1826 T2925 T2921 punct −,MCAD
R1827 T2926 T2927 det an,profile
R1828 T2927 T2920 dobj profile,developed
R1829 T2928 T2929 amod organic,acid
R1830 T2929 T2927 compound acid,profile
R1831 T2930 T2927 amod similar,profile
R1832 T2931 T2930 prep to,similar
R1833 T2932 T2933 npadvmod MCAD,deficient
R1834 T2933 T2935 amod deficient,patients
R1835 T2934 T2933 punct -,deficient
R1836 T2935 T2931 pobj patients,to
R1837 T2936 T2935 amod human,patients
R1838 T2937 T2918 punct .,revealed
R1839 T2939 T2940 advmod Specifically,developed
R1840 T2941 T2940 punct ", ",developed
R1841 T2942 T2943 advmod when,fasted
R1842 T2943 T2940 advcl fasted,developed
R1843 T2944 T2943 prep for,fasted
R1844 T2945 T2946 nummod 18,h
R1845 T2946 T2944 pobj h,for
R1846 T2947 T2940 punct ", ",developed
R1847 T2948 T2949 nmod MCAD,mice
R1848 T2949 T2940 nsubj mice,developed
R1849 T2950 T2948 punct −,MCAD
R1850 T2951 T2948 punct /,MCAD
R1851 T2952 T2948 punct −,MCAD
R1852 T2953 T2954 advmod significantly,elevated
R1853 T2954 T2955 amod elevated,concentrations
R1854 T2955 T2940 dobj concentrations,developed
R1855 T2956 T2955 prep of,concentrations
R1856 T2957 T2958 amod adipic,acids
R1857 T2958 T2956 pobj acids,of
R1858 T2959 T2957 punct ", ",adipic
R1859 T2960 T2957 conj suberic,adipic
R1860 T2961 T2960 punct ", ",suberic
R1861 T2962 T2960 cc and,suberic
R1862 T2963 T2960 conj sebacic,suberic
R1863 T2964 T2958 cc and,acids
R1864 T2965 T2958 conj hexanoylglycine,acids
R1865 T2966 T2967 mark as,compared
R1866 T2967 T2940 advcl compared,developed
R1867 T2968 T2967 prep to,compared
R1868 T2969 T2970 nmod MCAD,controls
R1869 T2970 T2968 pobj controls,to
R1870 T2971 T2969 punct +,MCAD
R1871 T2972 T2969 punct /,MCAD
R1872 T2973 T2969 punct +,MCAD
R1873 T2974 T2970 punct ", ",controls
R1874 T2975 T2976 dep which,showed
R1875 T2976 T2970 relcl showed,controls
R1876 T2977 T2976 dobj trace,showed
R1877 T2978 T2977 prep to,trace
R1878 T2979 T2980 det no,amounts
R1879 T2980 T2978 pobj amounts,to
R1880 T2981 T2980 amod detectable,amounts
R1881 T2982 T2977 prep of,trace
R1882 T2983 T2984 det the,acids
R1883 T2984 T2982 pobj acids,of
R1884 T2985 T2984 amod same,acids
R1885 T2986 T2984 amod organic,acids
R1886 T2987 T2988 punct (,Table
R1887 T2988 T2940 parataxis Table,developed
R1888 T2989 T2988 nummod 1,Table
R1889 T2990 T2988 punct ),Table
R1890 T2991 T2940 punct .,developed
R1891 T2993 T2994 amod Adipic,acid
R1892 T2994 T2995 nsubj acid,is
R1893 T2996 T2995 neg not,is
R1894 T2997 T2995 acomp specific,is
R1895 T2998 T2997 prep to,specific
R1896 T2999 T3000 compound MCAD,deficiency
R1897 T3000 T2998 pobj deficiency,to
R1898 T3001 T2995 punct .,is
R1899 T3003 T3004 nsubj We,evaluated
R1900 T3005 T3004 advmod also,evaluated
R1901 T3006 T3007 npadvmod β,hydroxybutyric
R1902 T3007 T3009 amod hydroxybutyric,concentrations
R1903 T3008 T3007 punct -,hydroxybutyric
R1904 T3009 T3004 dobj concentrations,evaluated
R1905 T3010 T3007 cc and,hydroxybutyric
R1906 T3011 T3007 conj acetoacetic,hydroxybutyric
R1907 T3012 T3004 cc and,evaluated
R1908 T3013 T3004 conj found,evaluated
R1909 T3014 T3015 det no,differences
R1910 T3015 T3013 dobj differences,found
R1911 T3016 T3015 amod significant,differences
R1912 T3017 T3015 prep between,differences
R1913 T3018 T3019 compound MCAD,genotypes
R1914 T3019 T3017 pobj genotypes,between
R1915 T3020 T3021 punct (,shown
R1916 T3021 T3013 parataxis shown,found
R1917 T3022 T3021 nsubj data,shown
R1918 T3023 T3021 neg not,shown
R1919 T3024 T3021 punct ),shown
R1920 T3025 T3004 punct .,evaluated
R1921 T3027 T3028 nsubj Comparison,revealed
R1922 T3029 T3027 prep of,Comparison
R1923 T3030 T3031 nmod MCAD,mice
R1924 T3031 T3029 pobj mice,of
R1925 T3032 T3030 punct +,MCAD
R1926 T3033 T3030 punct /,MCAD
R1927 T3034 T3030 punct +,MCAD
R1928 T3035 T3030 cc and,MCAD
R1929 T3036 T3030 conj MCAD,MCAD
R1930 T3037 T3036 punct −,MCAD
R1931 T3038 T3036 punct /,MCAD
R1932 T3039 T3036 punct −,MCAD
R1933 T3040 T3041 det no,differences
R1934 T3041 T3028 dobj differences,revealed
R1935 T3042 T3041 amod significant,differences
R1936 T3043 T3041 prep in,differences
R1937 T3044 T3045 amod total,concentrations
R1938 T3045 T3043 pobj concentrations,in
R1939 T3046 T3045 compound serum,concentrations
R1940 T3047 T3045 compound carnitine,concentrations
R1941 T3048 T3041 prep between,differences
R1942 T3049 T3050 nmod MCAD,mice
R1943 T3050 T3048 pobj mice,between
R1944 T3051 T3049 punct +,MCAD
R1945 T3052 T3049 punct /,MCAD
R1946 T3053 T3049 punct +,MCAD
R1947 T3054 T3049 cc and,MCAD
R1948 T3055 T3049 conj MCAD,MCAD
R1949 T3056 T3055 punct −,MCAD
R1950 T3057 T3055 punct /,MCAD
R1951 T3058 T3055 punct −,MCAD
R1952 T3059 T3028 punct ", ",revealed
R1953 T3060 T3028 cc but,revealed
R1954 T3061 T3062 nmod MCAD,mice
R1955 T3062 T3066 nsubj mice,had
R1956 T3063 T3061 punct −,MCAD
R1957 T3064 T3061 punct /,MCAD
R1958 T3065 T3061 punct −,MCAD
R1959 T3066 T3028 conj had,revealed
R1960 T3067 T3068 det a,elevation
R1961 T3068 T3075 nsubj elevation,evident
R1962 T3069 T3070 quantmod 5,6
R1963 T3070 T3073 quantmod 6,fold
R1964 T3071 T3070 punct -,6
R1965 T3072 T3070 quantmod to,6
R1966 T3073 T3068 nummod fold,elevation
R1967 T3074 T3073 punct -,fold
R1968 T3075 T3066 ccomp evident,had
R1969 T3076 T3068 prep of,elevation
R1970 T3077 T3078 compound serum,decenoylcarnitine
R1971 T3078 T3076 pobj decenoylcarnitine,of
R1972 T3079 T3075 prep in,evident
R1973 T3080 T3081 det the,profile
R1974 T3081 T3079 pobj profile,in
R1975 T3082 T3081 compound acylcarnitine,profile
R1976 T3083 T3084 punct (,Figure
R1977 T3084 T3066 parataxis Figure,had
R1978 T3085 T3084 nummod 4A,Figure
R1979 T3086 T3084 punct ),Figure
R1980 T3087 T3066 punct .,had
R1981 T3089 T3090 compound Bile,acylcarnitine
R1982 T3090 T3091 compound acylcarnitine,analysis
R1983 T3091 T3092 nsubj analysis,revealed
R1984 T3093 T3094 det a,pattern
R1985 T3094 T3092 dobj pattern,revealed
R1986 T3095 T3094 amod similar,pattern
R1987 T3096 T3094 compound acylcarnitine,pattern
R1988 T3097 T3094 prep as,pattern
R1989 T3098 T3097 prep in,as
R1990 T3099 T3098 pobj serum,in
R1991 T3100 T3101 punct (,Figure
R1992 T3101 T3092 parataxis Figure,revealed
R1993 T3102 T3101 nummod 4B,Figure
R1994 T3103 T3101 punct ),Figure
R1995 T3104 T3092 punct .,revealed
R1996 T3106 T3107 advmod However,are
R1997 T3108 T3107 punct ", ",are
R1998 T3109 T3110 det the,profiles
R1999 T3110 T3107 nsubj profiles,are
R2000 T3111 T3110 compound acylcarnitine,profiles
R2001 T3112 T3110 prep of,profiles
R2002 T3113 T3114 det the,mice
R2003 T3114 T3112 pobj mice,of
R2004 T3115 T3114 nmod MCAD,mice
R2005 T3116 T3115 punct −,MCAD
R2006 T3117 T3115 punct /,MCAD
R2007 T3118 T3115 punct −,MCAD
R2008 T3119 T3107 acomp different,are
R2009 T3120 T3119 prep from,different
R2010 T3121 T3120 pobj those,from
R2011 T3122 T3121 prep of,those
R2012 T3123 T3124 amod human,patients
R2013 T3124 T3122 pobj patients,of
R2014 T3125 T3126 npadvmod MCAD,deficient
R2015 T3126 T3124 amod deficient,patients
R2016 T3127 T3126 punct -,deficient
R2017 T3128 T3129 punct (,Figure
R2018 T3129 T3107 parataxis Figure,are
R2019 T3130 T3129 nummod 4C,Figure
R2020 T3131 T3129 punct ),Figure
R2021 T3132 T3107 punct .,are
R2022 T3134 T3135 amod Human,patients
R2023 T3135 T3136 nsubj patients,present
R2024 T3136 T3137 ccomp present,was
R2025 T3138 T3136 prep with,present
R2026 T3139 T3140 amod elevated,levels
R2027 T3140 T3138 pobj levels,with
R2028 T3141 T3140 prep of,levels
R2029 T3142 T3141 pobj C6,of
R2030 T3143 T3142 punct ", ",C6
R2031 T3144 T3142 conj C8,C6
R2032 T3145 T3144 punct ", ",C8
R2033 T3146 T3144 cc and,C8
R2034 T3147 T3144 conj C10,C8
R2035 T3148 T3147 punct :,C10
R2036 T3149 T3147 nummod 1,C10
R2037 T3150 T3142 appos acylcarnitines,C6
R2038 T3151 T3136 punct ", ",present
R2039 T3152 T3153 mark as,did
R2040 T3153 T3136 advcl did,present
R2041 T3154 T3155 det the,mice
R2042 T3155 T3153 nsubj mice,did
R2043 T3156 T3155 compound mutant,mice
R2044 T3157 T3137 punct ;,was
R2045 T3158 T3137 advmod however,was
R2046 T3159 T3137 punct ", ",was
R2047 T3160 T3161 det the,peak
R2048 T3161 T3137 nsubj peak,was
R2049 T3162 T3161 amod predominant,peak
R2050 T3163 T3164 compound C8,acylcarnitine
R2051 T3164 T3137 attr acylcarnitine,was
R2052 T3165 T3137 prep in,was
R2053 T3166 T3165 pobj humans,in
R2054 T3167 T3137 punct ", ",was
R2055 T3168 T3169 mark whereas,was
R2056 T3169 T3137 advcl was,was
R2057 T3170 T3169 prep in,was
R2058 T3171 T3172 det the,mouse
R2059 T3172 T3170 pobj mouse,in
R2060 T3173 T3169 nsubj it,was
R2061 T3174 T3175 nmod C10,acylcarnitine
R2062 T3175 T3169 attr acylcarnitine,was
R2063 T3176 T3174 punct :,C10
R2064 T3177 T3174 nummod 1,C10
R2065 T3178 T3137 punct .,was
R2070 T3319 T3320 amod Complete,examination
R2071 T3320 T3322 nsubj examination,demonstrated
R2072 T3321 T3320 amod histopathologic,examination
R2073 T3323 T3320 prep of,examination
R2074 T3324 T3325 nummod one,group
R2075 T3325 T3323 pobj group,of
R2076 T3326 T3325 prep of,group
R2077 T3327 T3328 nmod mutant,mice
R2078 T3328 T3326 pobj mice,of
R2079 T3329 T3327 cc and,mutant
R2080 T3330 T3327 conj MCAD,mutant
R2081 T3331 T3330 punct +,MCAD
R2082 T3332 T3330 punct /,MCAD
R2083 T3333 T3330 punct +,MCAD
R2084 T3334 T3328 compound control,mice
R2085 T3335 T3320 prep after,examination
R2086 T3336 T3337 det a,fast
R2087 T3337 T3335 pobj fast,after
R2088 T3338 T3339 nummod 24,h
R2089 T3339 T3337 compound h,fast
R2090 T3340 T3339 punct -,h
R2091 T3341 T3342 amod diffuse,steatosis
R2092 T3342 T3322 dobj steatosis,demonstrated
R2093 T3343 T3342 amod microvesicular,steatosis
R2094 T3344 T3343 cc and,microvesicular
R2095 T3345 T3343 conj macrovesicular,microvesicular
R2096 T3346 T3342 amod hepatic,steatosis
R2097 T3347 T3322 prep in,demonstrated
R2098 T3348 T3349 quantmod 6,8
R2099 T3349 T3351 nummod 8,wk
R2100 T3350 T3349 punct –,8
R2101 T3351 T3353 npadvmod wk,old
R2102 T3352 T3351 punct -,wk
R2103 T3353 T3355 amod old,mice
R2104 T3354 T3353 punct -,old
R2105 T3355 T3347 pobj mice,in
R2106 T3356 T3355 nmod MCAD,mice
R2107 T3357 T3356 punct −,MCAD
R2108 T3358 T3356 punct /,MCAD
R2109 T3359 T3356 punct −,MCAD
R2110 T3360 T3361 mark whereas,had
R2111 T3361 T3322 advcl had,demonstrated
R2112 T3362 T3363 nmod MCAD,mice
R2113 T3363 T3361 nsubj mice,had
R2114 T3364 T3362 punct +,MCAD
R2115 T3365 T3362 punct /,MCAD
R2116 T3366 T3362 punct +,MCAD
R2117 T3367 T3368 det no,changes
R2118 T3368 T3361 dobj changes,had
R2119 T3369 T3368 amod histologic,changes
R2120 T3370 T3371 punct (,5A
R2121 T3371 T3322 parataxis 5A,demonstrated
R2122 T3372 T3371 nmod Figure,5A
R2123 T3373 T3371 cc and,5A
R2124 T3374 T3371 conj 5B,5A
R2125 T3375 T3371 punct ),5A
R2126 T3376 T3322 punct .,demonstrated
R2127 T3378 T3379 prep In,were
R2128 T3380 T3381 det another,group
R2129 T3381 T3378 pobj group,In
R2130 T3382 T3381 prep of,group
R2131 T3383 T3384 nummod 4,wk
R2132 T3384 T3386 npadvmod wk,old
R2133 T3385 T3384 punct -,wk
R2134 T3386 T3388 amod old,mice
R2135 T3387 T3386 punct -,old
R2136 T3388 T3382 pobj mice,of
R2137 T3389 T3388 nmod MCAD,mice
R2138 T3390 T3389 punct +,MCAD
R2139 T3391 T3389 punct /,MCAD
R2140 T3392 T3389 punct +,MCAD
R2141 T3393 T3389 cc and,MCAD
R2142 T3394 T3389 conj MCAD,MCAD
R2143 T3395 T3394 punct −,MCAD
R2144 T3396 T3394 punct /,MCAD
R2145 T3397 T3394 punct −,MCAD
R2146 T3398 T3388 acl fasted,mice
R2147 T3399 T3398 prep for,fasted
R2148 T3400 T3401 nummod 24,h
R2149 T3401 T3399 pobj h,for
R2150 T3402 T3401 punct -,h
R2151 T3403 T3379 punct ", ",were
R2152 T3404 T3379 expl there,were
R2153 T3405 T3379 attr minimal,were
R2154 T3406 T3405 prep to,minimal
R2155 T3407 T3406 pobj no,to
R2156 T3408 T3405 appos abnormalities,minimal
R2157 T3409 T3379 prep in,were
R2158 T3410 T3411 det all,organs
R2159 T3411 T3409 pobj organs,in
R2160 T3412 T3411 acl evaluated,organs
R2161 T3413 T3379 punct .,were
R2162 T3415 T3416 advmod Only,mice
R2163 T3416 T3423 nsubj mice,demonstrated
R2164 T3417 T3416 det the,mice
R2165 T3418 T3416 amod older,mice
R2166 T3419 T3416 nmod MCAD,mice
R2167 T3420 T3419 punct −,MCAD
R2168 T3421 T3419 punct /,MCAD
R2169 T3422 T3419 punct −,MCAD
R2170 T3424 T3423 punct ", ",demonstrated
R2171 T3425 T3423 advmod therefore,demonstrated
R2172 T3426 T3423 punct ", ",demonstrated
R2173 T3427 T3428 amod hepatic,steatosis
R2174 T3428 T3423 dobj steatosis,demonstrated
R2175 T3429 T3423 punct .,demonstrated
R2176 T3431 T3432 prep In,found
R2177 T3433 T3431 pobj addition,In
R2178 T3434 T3432 punct ", ",found
R2179 T3435 T3432 nsubj we,found
R2180 T3436 T3437 amod sporadic,lesions
R2181 T3437 T3432 dobj lesions,found
R2182 T3438 T3437 amod cardiac,lesions
R2183 T3439 T3432 prep in,found
R2184 T3440 T3441 amod multiple,mice
R2185 T3441 T3439 pobj mice,in
R2186 T3442 T3441 nmod MCAD,mice
R2187 T3443 T3442 punct −,MCAD
R2188 T3444 T3442 punct /,MCAD
R2189 T3445 T3442 punct −,MCAD
R2190 T3446 T3432 punct .,found
R2191 T3448 T3449 prep In,had
R2192 T3450 T3451 nummod one,example
R2193 T3451 T3448 pobj example,In
R2194 T3452 T3449 punct ", ",had
R2195 T3453 T3454 det an,mouse
R2196 T3454 T3449 nsubj mouse,had
R2197 T3455 T3454 nmod MCAD,mouse
R2198 T3456 T3455 punct −,MCAD
R2199 T3457 T3455 punct /,MCAD
R2200 T3458 T3455 punct −,MCAD
R2201 T3459 T3460 amod diffuse,cardiomyopathy
R2202 T3460 T3449 dobj cardiomyopathy,had
R2203 T3461 T3460 prep with,cardiomyopathy
R2204 T3462 T3463 amod chronic,degeneration
R2205 T3463 T3461 pobj degeneration,with
R2206 T3464 T3463 amod active,degeneration
R2207 T3465 T3463 amod multifocal,degeneration
R2208 T3466 T3463 compound myocyte,degeneration
R2209 T3467 T3463 cc and,degeneration
R2210 T3468 T3463 conj necrosis,degeneration
R2211 T3469 T3470 punct (,5C
R2212 T3470 T3449 parataxis 5C,had
R2213 T3471 T3470 nmod Figure,5C
R2214 T3472 T3470 cc and,5C
R2215 T3473 T3470 conj 5D,5C
R2216 T3474 T3470 punct ),5C
R2217 T3475 T3449 punct .,had
R2218 T3477 T3478 nsubj Changes,included
R2219 T3479 T3477 prep in,Changes
R2220 T3480 T3481 amod degenerating,myocytes
R2221 T3481 T3479 pobj myocytes,in
R2222 T3482 T3478 dobj swelling,included
R2223 T3483 T3482 punct ", ",swelling
R2224 T3484 T3485 amod pale,staining
R2225 T3485 T3482 conj staining,swelling
R2226 T3486 T3485 punct ", ",staining
R2227 T3487 T3485 cc and,staining
R2228 T3488 T3485 punct ", ",staining
R2229 T3489 T3490 prep in,replacement
R2230 T3490 T3485 conj replacement,staining
R2231 T3491 T3489 pobj portions,in
R2232 T3492 T3491 prep of,portions
R2233 T3493 T3494 det the,sarcoplasm
R2234 T3494 T3492 pobj sarcoplasm,of
R2235 T3495 T3490 punct ", ",replacement
R2236 T3496 T3490 prep of,replacement
R2237 T3497 T3496 pobj myofibrils,of
R2238 T3498 T3490 prep with,replacement
R2239 T3499 T3500 advmod finely,granular
R2240 T3500 T3501 amod granular,material
R2241 T3501 T3498 pobj material,with
R2242 T3502 T3501 amod eosinophilic,material
R2243 T3503 T3478 punct .,included
R2244 T3505 T3506 nsubj Nuclei,were
R2245 T3507 T3505 prep of,Nuclei
R2246 T3508 T3509 amod affected,myocytes
R2247 T3509 T3507 pobj myocytes,of
R2248 T3510 T3506 acomp large,were
R2249 T3511 T3510 punct ", ",large
R2250 T3512 T3510 conj pale,large
R2251 T3513 T3512 punct ", ",pale
R2252 T3514 T3512 cc and,pale
R2253 T3515 T3512 conj vesicular,pale
R2254 T3516 T3506 cc and,were
R2255 T3517 T3506 conj had,were
R2256 T3518 T3519 amod prominent,nucleoli
R2257 T3519 T3517 dobj nucleoli,had
R2258 T3520 T3506 punct .,were
R2259 T3522 T3523 prep In,was
R2260 T3524 T3525 det the,areas
R2261 T3525 T3522 pobj areas,In
R2262 T3526 T3527 advmod most,severely
R2263 T3527 T3528 advmod severely,affected
R2264 T3528 T3525 amod affected,areas
R2265 T3529 T3523 punct ", ",was
R2266 T3530 T3523 expl there,was
R2267 T3531 T3523 attr loss,was
R2268 T3532 T3531 prep of,loss
R2269 T3533 T3532 pobj myocytes,of
R2270 T3534 T3531 acl accompanied,loss
R2271 T3535 T3534 agent by,accompanied
R2272 T3536 T3535 pobj fibrosis,by
R2273 T3537 T3523 punct .,was
R2274 T3539 T3540 prep In,was
R2275 T3541 T3542 det the,wall
R2276 T3542 T3539 pobj wall,In
R2277 T3543 T3542 prep of,wall
R2278 T3544 T3545 det the,aorta
R2279 T3545 T3543 pobj aorta,of
R2280 T3546 T3545 prep at,aorta
R2281 T3547 T3548 det the,base
R2282 T3548 T3546 pobj base,at
R2283 T3549 T3548 prep of,base
R2284 T3550 T3551 det the,heart
R2285 T3551 T3549 pobj heart,of
R2286 T3552 T3540 expl there,was
R2287 T3553 T3554 amod multifocal,degeneration
R2288 T3554 T3540 attr degeneration,was
R2289 T3555 T3554 prep of,degeneration
R2290 T3556 T3557 det the,tissue
R2291 T3557 T3555 pobj tissue,of
R2292 T3558 T3557 amod elastic,tissue
R2293 T3559 T3554 punct ", ",degeneration
R2294 T3560 T3554 acl accompanied,degeneration
R2295 T3561 T3560 agent by,accompanied
R2296 T3562 T3563 amod multifocal,collections
R2297 T3563 T3561 pobj collections,by
R2298 T3564 T3563 prep of,collections
R2299 T3565 T3566 amod globular,pigment
R2300 T3566 T3564 pobj pigment,of
R2301 T3567 T3566 amod translucent,pigment
R2302 T3568 T3569 amod yellow,brown
R2303 T3569 T3566 amod brown,pigment
R2304 T3570 T3569 punct -,brown
R2305 T3571 T3563 acl interpreted,collections
R2306 T3572 T3573 aux to,be
R2307 T3573 T3571 xcomp be,interpreted
R2308 T3574 T3575 compound ceroid,lipofuscin
R2309 T3575 T3573 attr lipofuscin,be
R2310 T3576 T3575 punct /,lipofuscin
R2311 T3577 T3540 punct .,was
R2312 T3579 T3580 amod Similar,deposits
R2313 T3580 T3581 nsubjpass deposits,scattered
R2314 T3582 T3581 auxpass were,scattered
R2315 T3583 T3581 prep within,scattered
R2316 T3584 T3585 amod adjacent,tissue
R2317 T3585 T3583 pobj tissue,within
R2318 T3586 T3585 compound adipose,tissue
R2319 T3587 T3581 punct .,scattered
R4678 T7248 T7247 prep for,Strategy
R4679 T7249 T7248 pobj Disruption,for
R4680 T7250 T7249 prep of,Disruption
R4681 T7251 T7252 det the,Gene
R4682 T7252 T7250 pobj Gene,of
R4683 T7253 T7252 compound Mouse,Gene
R4684 T7254 T7252 compound Acadm,Gene
R4685 T7256 T7257 punct (,A
R4686 T7257 T7258 meta A,vector
R4687 T7259 T7257 punct ),A
R4688 T7260 T7258 det The,vector
R4689 T7261 T7262 compound MCAD,insertion
R4690 T7262 T7258 compound insertion,vector
R4691 T7263 T7262 compound IV2,insertion
R4692 T7264 T7258 compound targeting,vector
R4693 T7265 T7258 prep with,vector
R4694 T7266 T7267 det a,region
R4695 T7267 T7265 pobj region,with
R4696 T7268 T7267 amod deleted,region
R4697 T7269 T7270 nummod 1.3,kb
R4698 T7270 T7267 compound kb,region
R4699 T7271 T7270 punct -,kb
R4700 T7272 T7267 acl encompassing,region
R4701 T7273 T7272 dobj exon,encompassing
R4702 T7274 T7273 nummod 10,exon
R4703 T7275 T7273 cc and,exon
R4704 T7276 T7277 amod flanking,sequences
R4705 T7277 T7273 conj sequences,exon
R4706 T7278 T7258 punct .,vector
R4707 T7280 T7281 compound MCAD,IV2
R4708 T7281 T7282 nsubj IV2,undergoes
R4709 T7283 T7284 compound gap,repair
R4710 T7284 T7282 dobj repair,undergoes
R4711 T7285 T7282 prep upon,undergoes
R4712 T7286 T7287 amod homologous,recombination
R4713 T7287 T7285 pobj recombination,upon
R4714 T7288 T7282 prep at,undergoes
R4715 T7289 T7290 det the,locus
R4716 T7290 T7288 pobj locus,at
R4717 T7291 T7290 amod endogenous,locus
R4718 T7292 T7290 compound Acadm,locus
R4719 T7293 T7282 advcl resulting,undergoes
R4720 T7294 T7293 prep in,resulting
R4721 T7295 T7296 det a,duplication
R4722 T7296 T7294 pobj duplication,in
R4723 T7297 T7296 prep of,duplication
R4724 T7298 T7299 nmod exons,8
R4725 T7299 T7297 pobj 8,of
R4726 T7300 T7299 punct ", ",8
R4727 T7301 T7299 conj 9,8
R4728 T7302 T7301 punct ", ",9
R4729 T7303 T7301 cc and,9
R4730 T7304 T7301 conj 10,9
R4731 T7305 T7296 prep at,duplication
R4732 T7306 T7307 det the,allele
R4733 T7307 T7305 pobj allele,at
R4734 T7308 T7307 amod disrupted,allele
R4735 T7309 T7282 punct .,undergoes
R4736 T7311 T7312 punct (,B
R4737 T7312 T7313 meta B,analysis
R4738 T7314 T7312 punct ),B
R4739 T7315 T7316 compound Southern,blot
R4740 T7316 T7313 compound blot,analysis
R4741 T7317 T7313 prep of,analysis
R4742 T7318 T7319 npadvmod EcoRI,digested
R4743 T7319 T7321 amod digested,DNA
R4744 T7320 T7319 punct -,digested
R4745 T7321 T7317 pobj DNA,of
R4746 T7322 T7321 amod genomic,DNA
R4747 T7323 T7321 prep from,DNA
R4748 T7324 T7325 compound ES,cells
R4749 T7325 T7323 pobj cells,from
R4750 T7326 T7325 acl screened,cells
R4751 T7327 T7326 prep by,screened
R4752 T7328 T7327 pobj PCR,by
R4753 T7329 T7313 punct .,analysis
R4754 T7331 T7332 compound Probe,A
R4755 T7332 T7333 nsubj A,hybridizes
R4756 T7334 T7332 punct ", ",A
R4757 T7335 T7336 det a,fragment
R4758 T7336 T7332 appos fragment,A
R4759 T7337 T7336 compound DNA,fragment
R4760 T7338 T7336 acl consisting,fragment
R4761 T7339 T7338 prep of,consisting
R4762 T7340 T7341 det a,portion
R4763 T7341 T7339 pobj portion,of
R4764 T7342 T7341 prep of,portion
R4765 T7343 T7342 pobj exon,of
R4766 T7344 T7343 nummod 10,exon
R4767 T7345 T7346 dep that,is
R4768 T7346 T7341 relcl is,portion
R4769 T7347 T7346 neg not,is
R4770 T7348 T7346 acomp present,is
R4771 T7349 T7346 prep in,is
R4772 T7350 T7351 det the,vector
R4773 T7351 T7349 pobj vector,in
R4774 T7352 T7351 compound targeting,vector
R4775 T7353 T7333 punct ", ",hybridizes
R4776 T7354 T7333 prep to,hybridizes
R4777 T7355 T7356 det an,fragment
R4778 T7356 T7354 pobj fragment,to
R4779 T7357 T7356 amod endogenous,fragment
R4780 T7358 T7359 nummod 3.1,kb
R4781 T7359 T7356 compound kb,fragment
R4782 T7360 T7359 punct -,kb
R4783 T7361 T7333 cc and,hybridizes
R4784 T7362 T7333 punct ", ",hybridizes
R4785 T7363 T7364 prep upon,to
R4786 T7364 T7333 conj to,hybridizes
R4787 T7365 T7366 amod homologous,recombination
R4788 T7366 T7363 pobj recombination,upon
R4789 T7367 T7364 punct ", ",to
R4790 T7368 T7369 det a,fragment
R4791 T7369 T7364 pobj fragment,to
R4792 T7370 T7371 nummod 13.2,kb
R4793 T7371 T7369 compound kb,fragment
R4794 T7372 T7371 punct -,kb
R4795 T7373 T7333 punct .,hybridizes
R4796 T7375 T7376 nsubj Lane,represents
R4797 T7377 T7375 nummod 1,Lane
R4798 T7378 T7379 det a,line
R4799 T7379 T7376 dobj line,represents
R4800 T7380 T7381 amod wild,type
R4801 T7381 T7379 compound type,line
R4802 T7382 T7381 punct -,type
R4803 T7383 T7379 compound ES,line
R4804 T7384 T7379 compound cell,line
R4805 T7385 T7376 punct ", ",represents
R4806 T7386 T7376 cc and,represents
R4807 T7387 T7388 nmod Lane,2
R4808 T7388 T7389 nsubj 2,represent
R4809 T7389 T7376 conj represent,represents
R4810 T7390 T7388 cc and,2
R4811 T7391 T7388 conj 3,2
R4812 T7392 T7393 amod targeted,lines
R4813 T7393 T7389 dobj lines,represent
R4814 T7394 T7393 compound ES,lines
R4815 T7395 T7393 compound cell,lines
R4816 T7396 T7389 punct .,represent
R4817 T7455 T7456 compound Northern,Blot
R4818 T7456 T7457 compound Blot,Analysis
R4819 T7458 T7457 prep from,Analysis
R4820 T7459 T7460 nmod MCAD,Mice
R4821 T7460 T7458 pobj Mice,from
R4822 T7461 T7459 punct −,MCAD
R4823 T7462 T7459 punct /,MCAD
R4824 T7463 T7459 punct −,MCAD
R4825 T7464 T7465 punct (,2
R4826 T7465 T7459 parataxis 2,MCAD
R4827 T7466 T7465 nsubj n,2
R4828 T7467 T7465 punct =,2
R4829 T7468 T7465 punct ),2
R4830 T7469 T7459 cc and,MCAD
R4831 T7470 T7459 conj MCAD,MCAD
R4832 T7471 T7470 punct +,MCAD
R4833 T7472 T7470 punct /,MCAD
R4834 T7473 T7470 punct +,MCAD
R4835 T7474 T7475 punct (,2
R4836 T7475 T7470 parataxis 2,MCAD
R4837 T7476 T7475 nsubj n,2
R4838 T7477 T7475 punct =,2
R4839 T7478 T7475 punct ),2
R4840 T7480 T7481 compound Acadm,message
R4841 T7481 T7482 nsubjpass message,detected
R4842 T7483 T7482 auxpass was,detected
R4843 T7484 T7482 prep from,detected
R4844 T7485 T7486 det the,heart
R4845 T7486 T7484 pobj heart,from
R4846 T7487 T7486 punct ", ",heart
R4847 T7488 T7486 conj liver,heart
R4848 T7489 T7488 punct ", ",liver
R4849 T7490 T7491 amod brown,fat
R4850 T7491 T7488 conj fat,liver
R4851 T7492 T7491 punct ", ",fat
R4852 T7493 T7491 conj brain,fat
R4853 T7494 T7493 punct ", ",brain
R4854 T7495 T7493 conj kidney,brain
R4855 T7496 T7495 punct ", ",kidney
R4856 T7497 T7495 cc and,kidney
R4857 T7498 T7495 conj muscle,kidney
R4858 T7499 T7486 punct (,heart
R4859 T7500 T7486 cc and,heart
R4860 T7501 T7502 amod white,fat
R4861 T7502 T7486 conj fat,heart
R4862 T7503 T7502 cc and,fat
R4863 T7504 T7502 conj testes,fat
R4864 T7505 T7506 punct ", ",shown
R4865 T7506 T7486 parataxis shown,heart
R4866 T7507 T7506 nsubj data,shown
R4867 T7508 T7506 neg not,shown
R4868 T7509 T7506 punct ),shown
R4869 T7510 T7486 prep of,heart
R4870 T7511 T7512 amod only,mice
R4871 T7512 T7510 pobj mice,of
R4872 T7513 T7512 nmod MCAD,mice
R4873 T7514 T7513 punct +,MCAD
R4874 T7515 T7513 punct /,MCAD
R4875 T7516 T7513 punct +,MCAD
R4876 T7517 T7482 punct .,detected
R4877 T7519 T7520 advmod Most,expression
R4878 T7520 T7522 nsubj expression,occurred
R4879 T7521 T7520 amod robust,expression
R4880 T7523 T7522 prep in,occurred
R4881 T7524 T7525 amod brown,fat
R4882 T7525 T7523 pobj fat,in
R4883 T7526 T7525 punct ", ",fat
R4884 T7527 T7525 conj kidney,fat
R4885 T7528 T7527 punct ", ",kidney
R4886 T7529 T7527 conj heart,kidney
R4887 T7530 T7529 punct ", ",heart
R4888 T7531 T7529 cc and,heart
R4889 T7532 T7533 amod skeletal,muscle
R4890 T7533 T7529 conj muscle,heart
R4891 T7534 T7522 punct .,occurred
R4892 T7536 T7537 nmod MCAD,mice
R4893 T7537 T7541 nsubj mice,had
R4894 T7538 T7536 punct −,MCAD
R4895 T7539 T7536 punct /,MCAD
R4896 T7540 T7536 punct −,MCAD
R4897 T7542 T7543 det no,message
R4898 T7543 T7541 dobj message,had
R4899 T7544 T7543 amod detectable,message
R4900 T7545 T7541 prep in,had
R4901 T7546 T7547 det all,tissues
R4902 T7547 T7545 pobj tissues,in
R4903 T7548 T7547 acl examined,tissues
R4904 T7549 T7541 punct .,had
R4905 T7639 T7638 prep of,Immunoblots
R4906 T7640 T7641 compound Liver,Homogenates
R4907 T7641 T7639 pobj Homogenates,of
R4908 T7642 T7638 prep from,Immunoblots
R4909 T7643 T7644 nmod MCAD,Mice
R4910 T7644 T7642 pobj Mice,from
R4911 T7645 T7643 punct +,MCAD
R4912 T7646 T7643 punct /,MCAD
R4913 T7647 T7643 punct +,MCAD
R4914 T7648 T7643 cc and,MCAD
R4915 T7649 T7643 conj MCAD,MCAD
R4916 T7650 T7649 punct −,MCAD
R4917 T7651 T7649 punct /,MCAD
R4918 T7652 T7649 punct −,MCAD
R4919 T7654 T7655 nsubjpass These,probed
R4920 T7656 T7655 auxpass were,probed
R4921 T7657 T7655 prep with,probed
R4922 T7658 T7659 amod anti-MCAD,antibody
R4923 T7659 T7657 pobj antibody,with
R4924 T7660 T7659 cc or,antibody
R4925 T7661 T7662 amod anti-SCAD,antibody
R4926 T7662 T7659 conj antibody,antibody
R4927 T7663 T7655 punct .,probed
R4928 T7665 T7666 amod Homozygous,mice
R4929 T7666 T7671 nsubj mice,had
R4930 T7667 T7666 nmod MCAD,mice
R4931 T7668 T7667 punct −,MCAD
R4932 T7669 T7667 punct /,MCAD
R4933 T7670 T7667 punct −,MCAD
R4934 T7672 T7673 det no,protein
R4935 T7673 T7671 dobj protein,had
R4936 T7674 T7673 amod detectable,protein
R4937 T7675 T7673 compound MCAD,protein
R4938 T7676 T7671 punct .,had
R4939 T7678 T7679 compound MCAD,protein
R4940 T7679 T7680 nsubj protein,is
R4941 T7681 T7682 advmod only,detectable
R4942 T7682 T7680 acomp detectable,is
R4943 T7683 T7680 prep under,is
R4944 T7684 T7685 det the,lane
R4945 T7685 T7683 pobj lane,under
R4946 T7686 T7685 nmod MCAD,lane
R4947 T7687 T7688 npadvmod protein,spiked
R4948 T7688 T7685 amod spiked,lane
R4949 T7689 T7688 punct –,spiked
R4950 T7690 T7691 punct (,control
R4951 T7691 T7685 parataxis control,lane
R4952 T7692 T7691 amod positive,control
R4953 T7693 T7691 punct ),control
R4954 T7694 T7680 punct .,is
R4955 T7696 T7697 prep As,revealed
R4956 T7698 T7699 det a,analysis
R4957 T7699 T7696 pobj analysis,As
R4958 T7700 T7699 compound control,analysis
R4959 T7701 T7697 punct ", ",revealed
R4960 T7702 T7703 compound liver,homogenates
R4961 T7703 T7697 nsubj homogenates,revealed
R4962 T7704 T7703 acl probed,homogenates
R4963 T7705 T7704 prep with,probed
R4964 T7706 T7707 amod anti-SCAD,antibody
R4965 T7707 T7705 pobj antibody,with
R4966 T7708 T7709 mark that,was
R4967 T7709 T7697 ccomp was,revealed
R4968 T7710 T7711 compound SCAD,protein
R4969 T7711 T7709 nsubj protein,was
R4970 T7712 T7709 acomp present,was
R4971 T7713 T7709 prep in,was
R4972 T7714 T7715 preconj both,MCAD
R4973 T7715 T7716 nmod MCAD,mice
R4974 T7716 T7713 pobj mice,in
R4975 T7717 T7715 punct +,MCAD
R4976 T7718 T7715 punct /,MCAD
R4977 T7719 T7715 punct +,MCAD
R4978 T7720 T7715 cc and,MCAD
R4979 T7721 T7715 conj MCAD,MCAD
R4980 T7722 T7721 punct −,MCAD
R4981 T7723 T7721 punct /,MCAD
R4982 T7724 T7721 punct −,MCAD
R4983 T7725 T7697 punct .,revealed
R4984 T7727 T7728 det No,protein
R4985 T7728 T7733 nsubjpass protein,detected
R4986 T7729 T7728 nmod MCAD,protein
R4987 T7730 T7731 amod positive,control
R4988 T7731 T7728 compound control,protein
R4989 T7732 T7731 punct -,control
R4990 T7734 T7733 auxpass is,detected
R4991 T7735 T7733 prep by,detected
R4992 T7736 T7737 amod anti-SCAD,antibodies
R4993 T7737 T7735 pobj antibodies,by
R4994 T7738 T7739 punct (,lane
R4995 T7739 T7733 parataxis lane,detected
R4996 T7740 T7739 compound MCAD,lane
R4997 T7741 T7739 punct ),lane
R4998 T7742 T7733 punct .,detected
R4999 T7745 T7744 punct ", ",mw
R5000 T7746 T7747 amod molecular,standards
R5001 T7747 T7744 appos standards,mw
R5002 T7748 T7747 compound weight,standards
R5003 T7749 T7744 punct .,mw
R5004 T7814 T7815 compound Acylcarnitine,Analyses
R5005 T7817 T7818 punct (,A
R5006 T7818 T7819 meta A,analysis
R5007 T7820 T7818 punct ),A
R5008 T7821 T7822 compound Serum,acylcarnitine
R5009 T7822 T7819 compound acylcarnitine,analysis
R5010 T7823 T7819 prep of,analysis
R5011 T7824 T7825 nmod MCAD,mice
R5012 T7825 T7823 pobj mice,of
R5013 T7826 T7824 punct +,MCAD
R5014 T7827 T7824 punct /,MCAD
R5015 T7828 T7824 punct +,MCAD
R5016 T7829 T7830 punct (,4
R5017 T7830 T7824 parataxis 4,MCAD
R5018 T7831 T7830 nsubj n,4
R5019 T7832 T7830 punct =,4
R5020 T7833 T7830 punct ),4
R5021 T7834 T7824 cc and,MCAD
R5022 T7835 T7824 conj MCAD,MCAD
R5023 T7836 T7835 punct −,MCAD
R5024 T7837 T7835 punct /,MCAD
R5025 T7838 T7835 punct −,MCAD
R5026 T7839 T7840 punct (,4
R5027 T7840 T7825 parataxis 4,mice
R5028 T7841 T7840 nsubj n,4
R5029 T7842 T7840 punct =,4
R5030 T7843 T7840 punct ),4
R5031 T7845 T7846 expl There,are
R5032 T7847 T7848 amod significant,elevations
R5033 T7848 T7846 attr elevations,are
R5034 T7849 T7846 prep in,are
R5035 T7850 T7851 compound acylcarnitine,species
R5036 T7851 T7849 pobj species,in
R5037 T7852 T7853 mark as,indicated
R5038 T7853 T7846 advcl indicated,are
R5039 T7854 T7853 prep in,indicated
R5040 T7855 T7856 nmod MCAD,mice
R5041 T7856 T7854 pobj mice,in
R5042 T7857 T7855 punct −,MCAD
R5043 T7858 T7855 punct /,MCAD
R5044 T7859 T7855 punct −,MCAD
R5045 T7860 T7846 punct .,are
R5046 T7862 T7863 nsubj Values,are
R5047 T7864 T7862 acl shown,Values
R5048 T7865 T7866 compound mean,values
R5049 T7866 T7863 attr values,are
R5050 T7867 T7866 punct ±,values
R5051 T7868 T7869 amod standard,deviation
R5052 T7869 T7866 appos deviation,values
R5053 T7870 T7869 punct (,deviation
R5054 T7871 T7869 appos SD,deviation
R5055 T7872 T7863 punct ),are
R5056 T7873 T7863 punct .,are
R5057 T7875 T7876 nsubj Asterisk,indicates
R5058 T7877 T7876 dobj p,indicates
R5059 T7878 T7879 punct <,0.002
R5060 T7879 T7877 amod 0.002,p
R5061 T7880 T7876 cc and,indicates
R5062 T7881 T7882 nsubj ‡,indicates
R5063 T7882 T7876 conj indicates,indicates
R5064 T7883 T7882 dobj p,indicates
R5065 T7884 T7885 punct <,0.01
R5066 T7885 T7883 amod 0.01,p
R5067 T7886 T7882 punct .,indicates
R5068 T7888 T7889 punct (,B
R5069 T7889 T7890 meta B,are
R5070 T7891 T7889 punct ),B
R5071 T7892 T7890 expl There,are
R5072 T7893 T7894 amod significant,elevations
R5073 T7894 T7890 attr elevations,are
R5074 T7895 T7894 prep in,elevations
R5075 T7896 T7897 compound bile,acylcarnitines
R5076 T7897 T7895 pobj acylcarnitines,in
R5077 T7898 T7897 prep of,acylcarnitines
R5078 T7899 T7900 det the,mice
R5079 T7900 T7898 pobj mice,of
R5080 T7901 T7900 amod same,mice
R5081 T7902 T7900 acl shown,mice
R5082 T7903 T7902 prep in,shown
R5083 T7904 T7903 punct (,in
R5084 T7905 T7903 pobj A,in
R5085 T7906 T7902 punct ),shown
R5086 T7907 T7908 mark as,indicated
R5087 T7908 T7902 advcl indicated,shown
R5088 T7909 T7890 punct .,are
R5089 T7911 T7912 nsubj Values,are
R5090 T7913 T7911 acl shown,Values
R5091 T7914 T7915 compound mean,values
R5092 T7915 T7912 attr values,are
R5093 T7916 T7915 punct ±,values
R5094 T7917 T7915 appos SD,values
R5095 T7918 T7912 punct .,are
R5096 T7920 T7921 nsubj Asterisk,indicates
R5097 T7922 T7921 dobj p,indicates
R5098 T7923 T7924 punct <,0.001
R5099 T7924 T7922 amod 0.001,p
R5100 T7925 T7921 punct .,indicates
R5101 T7927 T7928 punct (,C
R5102 T7928 T7929 meta C,profile
R5103 T7930 T7928 punct ),C
R5104 T7931 T7932 compound Bile,acylcarnitine
R5105 T7932 T7929 compound acylcarnitine,profile
R5106 T7933 T7929 prep of,profile
R5107 T7934 T7935 det an,mouse
R5108 T7935 T7933 pobj mouse,of
R5109 T7936 T7935 nmod MCAD,mouse
R5110 T7937 T7936 punct −,MCAD
R5111 T7938 T7936 punct /,MCAD
R5112 T7939 T7936 punct −,MCAD
R5113 T7940 T7929 prep compared,profile
R5114 T7941 T7940 prep to,compared
R5115 T7942 T7943 det a,patient
R5116 T7943 T7941 pobj patient,to
R5117 T7944 T7943 amod human,patient
R5118 T7945 T7943 prep with,patient
R5119 T7946 T7947 compound MCAD,deficiency
R5120 T7947 T7945 pobj deficiency,with
R5121 T7948 T7929 punct .,profile
R5122 T7950 T7951 amod Internal,standards
R5123 T7951 T7952 nsubjpass standards,indicated
R5124 T7953 T7952 auxpass are,indicated
R5125 T7954 T7952 prep by,indicated
R5126 T7955 T7956 det an,asterisk
R5127 T7956 T7954 pobj asterisk,by
R5128 T7957 T7952 punct .,indicated
R5129 T8010 T8009 prep of,Histopathology
R5130 T8011 T8012 nmod MCAD,Mice
R5131 T8012 T8010 pobj Mice,of
R5132 T8013 T8011 punct +,MCAD
R5133 T8014 T8011 punct /,MCAD
R5134 T8015 T8011 punct +,MCAD
R5135 T8016 T8011 cc and,MCAD
R5136 T8017 T8011 conj MCAD,MCAD
R5137 T8018 T8017 punct −,MCAD
R5138 T8019 T8017 punct /,MCAD
R5139 T8020 T8017 punct −,MCAD
R5140 T8022 T8023 punct (,A
R5141 T8023 T8024 meta A,had
R5142 T8025 T8023 punct ),A
R5143 T8026 T8027 nmod MCAD,mice
R5144 T8027 T8024 nsubj mice,had
R5145 T8028 T8026 punct +,MCAD
R5146 T8029 T8026 punct /,MCAD
R5147 T8030 T8026 punct +,MCAD
R5148 T8031 T8032 det no,evidence
R5149 T8032 T8024 dobj evidence,had
R5150 T8033 T8032 prep of,evidence
R5151 T8034 T8035 amod hepatic,steatosis
R5152 T8035 T8033 pobj steatosis,of
R5153 T8036 T8024 prep following,had
R5154 T8037 T8038 det a,fast
R5155 T8038 T8036 pobj fast,following
R5156 T8039 T8040 nummod 24,h
R5157 T8040 T8038 compound h,fast
R5158 T8041 T8040 punct -,h
R5159 T8042 T8024 punct .,had
R5160 T8044 T8045 compound Liver,section
R5161 T8046 T8045 prep with,section
R5162 T8047 T8048 nmod Oil,O
R5163 T8048 T8051 compound O,stain
R5164 T8049 T8048 punct -,O
R5165 T8050 T8048 amod Red,O
R5166 T8051 T8046 pobj stain,with
R5167 T8052 T8045 punct .,section
R5168 T8054 T8055 punct (,B
R5169 T8055 T8056 meta B,Hepatosteatosis
R5170 T8057 T8055 punct ),B
R5171 T8058 T8056 prep in,Hepatosteatosis
R5172 T8059 T8060 compound MCAD,−
R5173 T8060 T8063 compound −,mouse
R5174 T8061 T8060 compound −,−
R5175 T8062 T8060 punct /,−
R5176 T8063 T8058 pobj mouse,in
R5177 T8064 T8056 prep following,Hepatosteatosis
R5178 T8065 T8066 det a,fast
R5179 T8066 T8064 pobj fast,following
R5180 T8067 T8068 nummod 24,h
R5181 T8068 T8066 compound h,fast
R5182 T8069 T8068 punct -,h
R5183 T8070 T8056 punct .,Hepatosteatosis
R5184 T8072 T8073 nmod Oil,O
R5185 T8073 T8076 npadvmod O,stained
R5186 T8074 T8073 punct -,O
R5187 T8075 T8073 amod Red,O
R5188 T8076 T8077 amod stained,sections
R5189 T8077 T8079 nsubj sections,revealed
R5190 T8078 T8077 compound liver,sections
R5191 T8080 T8081 amod severe,steatosis
R5192 T8081 T8079 dobj steatosis,revealed
R5193 T8082 T8080 cc and,severe
R5194 T8083 T8080 conj diffuse,severe
R5195 T8084 T8081 amod microvesicular,steatosis
R5196 T8085 T8084 cc and,microvesicular
R5197 T8086 T8084 conj macrovesicular,microvesicular
R5198 T8087 T8081 amod hepatic,steatosis
R5199 T8088 T8079 prep in,revealed
R5200 T8089 T8090 nmod MCAD,mice
R5201 T8090 T8088 pobj mice,in
R5202 T8091 T8089 punct −,MCAD
R5203 T8092 T8089 punct /,MCAD
R5204 T8093 T8089 punct −,MCAD
R5205 T8094 T8079 punct .,revealed
R5206 T8096 T8097 punct (,C
R5207 T8097 T8098 meta C,cardiomyopathy
R5208 T8099 T8097 cc and,C
R5209 T8100 T8097 conj D,C
R5210 T8101 T8100 punct ),D
R5211 T8102 T8098 amod Diffuse,cardiomyopathy
R5212 T8103 T8098 prep with,cardiomyopathy
R5213 T8104 T8105 amod chronic,degeneration
R5214 T8105 T8103 pobj degeneration,with
R5215 T8106 T8105 amod active,degeneration
R5216 T8107 T8105 amod multifocal,degeneration
R5217 T8108 T8105 compound myocyte,degeneration
R5218 T8109 T8105 cc and,degeneration
R5219 T8110 T8105 conj necrosis,degeneration
R5220 T8111 T8098 prep in,cardiomyopathy
R5221 T8112 T8113 nmod MCAD,mice
R5222 T8113 T8111 pobj mice,in
R5223 T8114 T8112 punct −,MCAD
R5224 T8115 T8112 punct /,MCAD
R5225 T8116 T8112 punct −,MCAD
R5226 T8117 T8098 punct .,cardiomyopathy
R5227 T8162 T8161 prep of,Characteristics
R5228 T8163 T8164 npadvmod MCAD,Deficient
R5229 T8164 T8166 amod Deficient,Mice
R5230 T8165 T8164 punct -,Deficient
R5231 T8166 T8162 pobj Mice,of
R579 T964 T965 compound Gene,Targeting
R580 T966 T965 cc and,Targeting
R581 T967 T965 conj Generation,Targeting
R582 T968 T967 prep of,Generation
R583 T969 T970 npadvmod MCAD,Deficient
R584 T970 T972 amod Deficient,Mice
R585 T971 T970 punct -,Deficient
R586 T972 T968 pobj Mice,of
R587 T974 T975 compound MCAD,vector
R588 T975 T977 nsubjpass vector,designed
R589 T976 T975 compound insertion,vector
R590 T978 T975 punct (,vector
R591 T979 T980 compound MCAD,IV2
R592 T980 T975 appos IV2,vector
R593 T981 T977 punct ),designed
R594 T982 T977 auxpass was,designed
R595 T983 T984 aux to,undergo
R596 T984 T977 advcl undergo,designed
R597 T985 T986 compound gap,repair
R598 T986 T984 dobj repair,undergo
R599 T987 T986 prep of,repair
R600 T988 T989 det the,region
R601 T989 T987 pobj region,of
R602 T990 T991 nummod 1.3,kb
R603 T991 T989 nmod kb,region
R604 T992 T991 punct -,kb
R605 T993 T989 amod deleted,region
R606 T994 T984 prep upon,undergo
R607 T995 T996 amod homologous,recombination
R608 T996 T994 pobj recombination,upon
R609 T997 T984 prep in,undergo
R610 T998 T999 nmod 129P2,cells
R611 T999 T997 pobj cells,in
R612 T1000 T1001 punct (,OlaHsd
R613 T1001 T999 parataxis OlaHsd,cells
R614 T1002 T1001 compound 129P2,OlaHsd
R615 T1003 T1001 punct /,OlaHsd
R616 T1004 T1001 punct ),OlaHsd
R617 T1005 T999 compound ES,cells
R618 T1006 T999 appos E14,cells
R619 T1007 T1006 punct –,E14
R620 T1008 T1006 nummod 1,E14
R621 T1009 T977 punct .,designed
R622 T1011 T1012 amod Correct,targeting
R623 T1012 T1013 nsubj targeting,resulted
R624 T1014 T1012 prep of,targeting
R625 T1015 T1016 det the,locus
R626 T1016 T1014 pobj locus,of
R627 T1017 T1016 compound MCAD,locus
R628 T1018 T1013 prep in,resulted
R629 T1019 T1020 det a,duplication
R630 T1020 T1018 pobj duplication,in
R631 T1021 T1020 prep of,duplication
R632 T1022 T1023 nmod exons,8
R633 T1023 T1021 pobj 8,of
R634 T1024 T1023 punct ", ",8
R635 T1025 T1023 conj 9,8
R636 T1026 T1025 punct ", ",9
R637 T1027 T1025 cc and,9
R638 T1028 T1025 conj 10,9
R639 T1029 T1020 cc and,duplication
R640 T1030 T1020 conj integration,duplication
R641 T1031 T1030 prep of,integration
R642 T1032 T1033 amod flanking,plasmid
R643 T1033 T1031 pobj plasmid,of
R644 T1034 T1033 cc and,plasmid
R645 T1035 T1036 compound Neo,sequences
R646 T1036 T1033 conj sequences,plasmid
R647 T1037 T1038 punct (,Figure
R648 T1038 T1013 parataxis Figure,resulted
R649 T1039 T1038 nummod 1A,Figure
R650 T1040 T1038 punct ),Figure
R651 T1041 T1013 punct .,resulted
R652 T1043 T1044 det The,vector
R653 T1044 T1046 nsubjpass vector,designed
R654 T1045 T1044 compound insertion,vector
R655 T1047 T1046 auxpass was,designed
R656 T1048 T1049 aux to,duplicate
R657 T1049 T1046 advcl duplicate,designed
R658 T1050 T1051 nmod exon,8
R659 T1051 T1049 dobj 8,duplicate
R660 T1052 T1051 punct ", ",8
R661 T1053 T1051 conj 9,8
R662 T1054 T1053 punct ", ",9
R663 T1055 T1053 cc and,9
R664 T1056 T1053 conj 10,9
R665 T1057 T1049 prep at,duplicate
R666 T1058 T1059 det the,locus
R667 T1059 T1057 pobj locus,at
R668 T1060 T1059 compound MCAD,locus
R669 T1061 T1046 punct .,designed
R670 T1063 T1064 nsubj Translation,results
R671 T1065 T1063 prep of,Translation
R672 T1066 T1067 det the,region
R673 T1067 T1065 pobj region,of
R674 T1068 T1067 amod duplicated,region
R675 T1069 T1067 nmod exon,region
R676 T1070 T1069 nummod 8,exon
R677 T1071 T1064 prep in,results
R678 T1072 T1073 det the,formation
R679 T1073 T1071 pobj formation,in
R680 T1074 T1073 prep of,formation
R681 T1075 T1076 amod premature,codons
R682 T1076 T1074 pobj codons,of
R683 T1077 T1076 compound stop,codons
R684 T1078 T1073 acl resulting,formation
R685 T1079 T1078 prep in,resulting
R686 T1080 T1079 pobj truncation,in
R687 T1081 T1080 prep of,truncation
R688 T1082 T1083 det the,monomer
R689 T1083 T1081 pobj monomer,of
R690 T1084 T1083 compound MCAD,monomer
R691 T1085 T1064 punct .,results
R692 T1087 T1088 advmod Specifically,arises
R693 T1089 T1088 punct ", ",arises
R694 T1090 T1091 det the,codon
R695 T1091 T1088 nsubj codon,arises
R696 T1092 T1091 amod first,codon
R697 T1093 T1091 amod premature,codon
R698 T1094 T1091 compound stop,codon
R699 T1095 T1088 prep after,arises
R700 T1096 T1095 pobj translation,after
R701 T1097 T1096 prep of,translation
R702 T1098 T1099 advmod only,seven
R703 T1099 T1100 nummod seven,acids
R704 T1100 T1097 pobj acids,of
R705 T1101 T1100 compound amino,acids
R706 T1102 T1100 prep from,acids
R707 T1103 T1104 det the,exon
R708 T1104 T1102 pobj exon,from
R709 T1105 T1104 amod duplicated,exon
R710 T1106 T1104 nummod 8,exon
R711 T1107 T1088 punct .,arises
R712 T1109 T1110 det The,monomer
R713 T1110 T1113 nsubj monomer,missing
R714 T1111 T1110 amod resulting,monomer
R715 T1112 T1110 compound MCAD,monomer
R716 T1114 T1113 aux is,missing
R717 T1115 T1116 det the,helixes
R718 T1116 T1113 dobj helixes,missing
R719 T1117 T1118 npadvmod C,terminal
R720 T1118 T1120 amod terminal,domain
R721 T1119 T1118 punct -,terminal
R722 T1120 T1116 compound domain,helixes
R723 T1121 T1116 compound α,helixes
R724 T1122 T1116 punct -,helixes
R725 T1123 T1124 dep that,are
R726 T1124 T1116 relcl are,helixes
R727 T1125 T1124 acomp responsible,are
R728 T1126 T1125 prep for,responsible
R729 T1127 T1126 pcomp making,for
R730 T1128 T1129 compound intersubunit,contacts
R731 T1129 T1127 dobj contacts,making
R732 T1130 T1131 aux to,generate
R733 T1131 T1127 advcl generate,making
R734 T1132 T1133 det the,homotetramer
R735 T1133 T1131 dobj homotetramer,generate
R736 T1134 T1133 amod functional,homotetramer
R737 T1135 T1133 compound MCAD,homotetramer
R738 T1136 T1113 punct .,missing
R739 T1138 T1139 compound ES,cell
R740 T1139 T1140 compound cell,clones
R741 T1140 T1141 nsubjpass clones,screened
R742 T1142 T1141 auxpass were,screened
R743 T1143 T1141 prep by,screened
R744 T1144 T1143 pobj PCR,by
R745 T1145 T1146 punct (,shown
R746 T1146 T1141 parataxis shown,screened
R747 T1147 T1146 nsubj data,shown
R748 T1148 T1146 neg not,shown
R749 T1149 T1146 punct ),shown
R750 T1150 T1141 cc and,screened
R751 T1151 T1141 conj confirmed,screened
R752 T1152 T1151 prep by,confirmed
R753 T1153 T1154 compound Southern,blot
R754 T1154 T1155 compound blot,analysis
R755 T1155 T1152 pobj analysis,by
R756 T1156 T1141 punct .,screened
R757 T1158 T1159 compound Southern,blot
R758 T1159 T1160 compound blot,analysis
R759 T1160 T1161 nsubj analysis,used
R760 T1162 T1163 det an,probe
R761 T1163 T1161 dobj probe,used
R762 T1164 T1163 nmod exon,probe
R763 T1165 T1164 nummod 10,exon
R764 T1166 T1167 punct (,A
R765 T1167 T1163 parataxis A,probe
R766 T1168 T1167 compound probe,A
R767 T1169 T1167 punct ),A
R768 T1170 T1163 punct ", ",probe
R769 T1171 T1172 neg not,present
R770 T1172 T1163 relcl present,probe
R771 T1173 T1172 prep in,present
R772 T1174 T1175 det the,vector
R773 T1175 T1173 pobj vector,in
R774 T1176 T1175 compound targeting,vector
R775 T1177 T1163 punct ", ",probe
R776 T1178 T1163 acl hybridized,probe
R777 T1179 T1178 prep to,hybridized
R778 T1180 T1181 det a,band
R779 T1181 T1179 pobj band,to
R780 T1182 T1183 nummod 13.2,kb
R781 T1183 T1181 compound kb,band
R782 T1184 T1183 punct -,kb
R783 T1185 T1178 prep in,hybridized
R784 T1186 T1185 pobj addition,in
R785 T1187 T1186 prep to,addition
R786 T1188 T1189 det the,band
R787 T1189 T1187 pobj band,to
R788 T1190 T1191 nummod 3.1,kb
R789 T1191 T1189 nmod kb,band
R790 T1192 T1191 punct -,kb
R791 T1193 T1189 amod endogenous,band
R792 T1194 T1189 acl indicating,band
R793 T1195 T1196 amod targeted,insertion
R794 T1196 T1194 dobj insertion,indicating
R795 T1197 T1196 prep of,insertion
R796 T1198 T1199 det the,vector
R797 T1199 T1197 pobj vector,of
R798 T1200 T1196 prep at,insertion
R799 T1201 T1202 det the,locus
R800 T1202 T1200 pobj locus,at
R801 T1203 T1202 compound Acadm,locus
R802 T1204 T1205 punct (,Figure
R803 T1205 T1161 parataxis Figure,used
R804 T1206 T1205 nummod 1B,Figure
R805 T1207 T1205 punct ),Figure
R806 T1208 T1161 punct .,used
R807 T1210 T1211 advmod Correctly,targeted
R808 T1211 T1212 amod targeted,clones
R809 T1212 T1215 nsubjpass clones,microinjected
R810 T1213 T1214 compound ES,cell
R811 T1214 T1212 compound cell,clones
R812 T1216 T1215 auxpass were,microinjected
R813 T1217 T1215 prep into,microinjected
R814 T1218 T1219 nmod B6,blastocysts
R815 T1219 T1217 pobj blastocysts,into
R816 T1220 T1219 punct (,blastocysts
R817 T1221 T1222 nmod C57BL,6NTac
R818 T1222 T1219 nmod 6NTac,blastocysts
R819 T1223 T1222 punct /,6NTac
R820 T1224 T1219 punct ),blastocysts
R821 T1225 T1226 aux to,generate
R822 T1226 T1215 advcl generate,microinjected
R823 T1227 T1228 amod chimeric,mice
R824 T1228 T1226 dobj mice,generate
R825 T1229 T1215 punct .,microinjected
R826 T1231 T1232 amod Chimeric,mice
R827 T1232 T1233 nsubjpass mice,backcrossed
R828 T1234 T1233 auxpass were,backcrossed
R829 T1235 T1233 prep to,backcrossed
R830 T1236 T1237 preconj both,129P2
R831 T1237 T1238 npadvmod 129P2,inbred
R832 T1238 T1241 amod inbred,mice
R833 T1239 T1237 cc and,129P2
R834 T1240 T1237 conj B6,129P2
R835 T1241 T1235 pobj mice,to
R836 T1242 T1243 aux to,produce
R837 T1243 T1233 advcl produce,backcrossed
R838 T1244 T1245 nmod MCAD,mice
R839 T1245 T1243 dobj mice,produce
R840 T1246 T1244 punct +,MCAD
R841 T1247 T1244 punct /,MCAD
R842 T1248 T1244 punct −,MCAD
R843 T1249 T1244 cc and,MCAD
R844 T1250 T1251 advmod eventually,MCAD
R845 T1251 T1244 conj MCAD,MCAD
R846 T1252 T1251 punct −,MCAD
R847 T1253 T1251 punct /,MCAD
R848 T1254 T1251 punct −,MCAD
R849 T1255 T1243 prep on,produce
R850 T1256 T1257 det a,background
R851 T1257 T1255 pobj background,on
R852 T1258 T1257 nmod B6,background
R853 T1259 T1258 punct /,B6
R854 T1260 T1258 nummod 129,B6
R855 T1261 T1257 amod mixed,background
R856 T1262 T1233 punct .,backcrossed
R857 T1264 T1265 det The,studies
R858 T1265 T1266 nsubjpass studies,conducted
R859 T1267 T1265 acl described,studies
R860 T1268 T1267 advmod here,described
R861 T1269 T1266 auxpass were,conducted
R862 T1270 T1266 advmod exclusively,conducted
R863 T1271 T1266 prep on,conducted
R864 T1272 T1273 det the,background
R865 T1273 T1271 pobj background,on
R866 T1274 T1273 nmod B6,background
R867 T1275 T1274 punct /,B6
R868 T1276 T1274 nummod 129,B6
R869 T1277 T1273 amod mixed,background
R870 T1278 T1273 prep compared,background
R871 T1279 T1278 prep with,compared
R872 T1280 T1281 compound littermate,controls
R873 T1281 T1279 pobj controls,with
R874 T1282 T1281 cc or,controls
R875 T1283 T1284 nmod B6,groups
R876 T1284 T1281 conj groups,controls
R877 T1285 T1283 punct /,B6
R878 T1286 T1283 nummod 129,B6
R879 T1287 T1284 compound control,groups
R880 T1288 T1281 acl maintained,controls
R881 T1289 T1288 prep by,maintained
R882 T1290 T1289 pobj intercrosses,by
R883 T1291 T1292 mark as,were
R884 T1292 T1288 advcl were,maintained
R885 T1293 T1294 det the,mutants
R886 T1294 T1292 nsubj mutants,were
R887 T1295 T1266 punct .,conducted
R888 T1297 T1298 csubj Perpetuating,proved
R889 T1299 T1300 det this,mutation
R890 T1300 T1297 dobj mutation,Perpetuating
R891 T1301 T1297 prep as,Perpetuating
R892 T1302 T1303 det a,line
R893 T1303 T1301 pobj line,as
R894 T1304 T1303 amod congenic,line
R895 T1305 T1303 compound mutant,line
R896 T1306 T1303 prep on,line
R897 T1307 T1308 det the,background
R898 T1308 T1306 pobj background,on
R899 T1309 T1308 compound 129P2,background
R900 T1310 T1298 oprd impractical,proved
R901 T1311 T1298 punct .,proved
R902 T1313 T1314 det The,mice
R903 T1314 T1316 nsubj mice,were
R904 T1315 T1314 compound 129P2,mice
R905 T1317 T1318 amod poor,breeders
R906 T1318 T1316 attr breeders,were
R907 T1319 T1316 prep as,were
R908 T1320 T1321 amod wild,types
R909 T1321 T1319 pobj types,as
R910 T1322 T1321 punct -,types
R911 T1323 T1316 punct ", ",were
R912 T1324 T1316 cc and,were
R913 T1325 T1326 advmod when,introduced
R914 T1326 T1327 advcl introduced,lost
R915 T1327 T1316 conj lost,were
R916 T1328 T1327 punct ", ",lost
R917 T1329 T1330 det the,mutation
R918 T1330 T1327 nsubjpass mutation,lost
R919 T1331 T1330 compound Acadm,mutation
R920 T1332 T1327 auxpass was,lost
R921 T1333 T1327 advmod nearly,lost
R922 T1334 T1327 prep on,lost
R923 T1335 T1336 det this,background
R924 T1336 T1334 pobj background,on
R925 T1337 T1327 prep because,lost
R926 T1338 T1337 pcomp of,because
R927 T1339 T1340 det the,rate
R928 T1340 T1337 pobj rate,because
R929 T1341 T1340 amod high,rate
R930 T1342 T1340 prep of,rate
R931 T1343 T1344 amod neonatal,death
R932 T1344 T1342 pobj death,of
R933 T1345 T1327 punct .,lost
R934 T1347 T1348 prep Because,proved
R935 T1349 T1347 pcomp of,Because
R936 T1350 T1351 det the,structure
R937 T1351 T1347 pobj structure,Because
R938 T1352 T1351 amod molecular,structure
R939 T1353 T1351 prep of,structure
R940 T1354 T1355 det the,allele
R941 T1355 T1353 pobj allele,of
R942 T1356 T1355 amod targeted,allele
R943 T1357 T1348 punct ", ",proved
R944 T1358 T1348 nsubj it,proved
R945 T1359 T1360 advmod virtually,impossible
R946 T1360 T1348 oprd impossible,proved
R947 T1361 T1362 aux to,distinguish
R948 T1362 T1348 xcomp distinguish,proved
R949 T1363 T1364 det all,genotypes
R950 T1364 T1362 dobj genotypes,distinguish
R951 T1365 T1364 nummod three,genotypes
R952 T1366 T1364 amod potential,genotypes
R953 T1367 T1348 punct .,proved
R954 T1369 T1370 nsubj We,detect
R955 T1370 T1373 ccomp detect,determined
R956 T1371 T1370 aux could,detect
R957 T1372 T1370 advmod clearly,detect
R958 T1374 T1375 det the,presence
R959 T1375 T1370 dobj presence,detect
R960 T1376 T1375 cc or,presence
R961 T1377 T1375 conj absence,presence
R962 T1378 T1375 prep of,presence
R963 T1379 T1380 det the,allele
R964 T1380 T1378 pobj allele,of
R965 T1381 T1380 amod targeted,allele
R966 T1382 T1373 punct ", ",determined
R967 T1383 T1373 advmod however,determined
R968 T1384 T1373 punct ", ",determined
R969 T1385 T1386 mark whether,was
R970 T1386 T1373 csubjpass was,determined
R971 T1387 T1388 det a,mouse
R972 T1388 T1386 nsubj mouse,was
R973 T1389 T1388 amod particular,mouse
R974 T1390 T1386 attr MCAD,was
R975 T1391 T1390 punct −,MCAD
R976 T1392 T1390 punct /,MCAD
R977 T1393 T1390 punct −,MCAD
R978 T1394 T1390 cc or,MCAD
R979 T1395 T1390 conj MCAD,MCAD
R980 T1396 T1395 punct +,MCAD
R981 T1397 T1395 punct /,MCAD
R982 T1398 T1395 punct −,MCAD
R983 T1399 T1373 aux could,determined
R984 T1400 T1373 neg not,determined
R985 T1401 T1373 auxpass be,determined
R986 T1402 T1373 prep by,determined
R987 T1403 T1404 compound Southern,blot
R988 T1404 T1402 pobj blot,by
R989 T1405 T1404 cc or,blot
R990 T1406 T1404 conj PCR,blot
R991 T1407 T1404 prep of,blot
R992 T1408 T1409 amod genomic,DNA
R993 T1409 T1407 pobj DNA,of
R994 T1410 T1373 punct .,determined
R995 T1412 T1413 advmod Ultimately,ascertained
R996 T1414 T1415 nmod MCAD,mice
R997 T1415 T1413 nsubjpass mice,ascertained
R998 T1416 T1414 punct −,MCAD
R999 T1417 T1414 punct /,MCAD