PMC:1189074 JSON TXT 8 Projects

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Id	Subject	Object	Predicate	Lexical cue
T43	0-6	JJ	denotes	Medium
T45	0-68	sentence	denotes	Medium-Chain Acyl-CoA Dehydrogenase Deficiency in Gene-Targeted Mice
T46	6-7	HYPH	denotes	-
T44	7-12	NN	denotes	Chain
T48	13-17	NN	denotes	Acyl
T50	17-18	HYPH	denotes	-
T49	18-21	NN	denotes	CoA
T47	22-35	NN	denotes	Dehydrogenase
T51	36-46	NN	denotes	Deficiency
T52	47-49	IN	denotes	in
T53	50-54	NN	denotes	Gene
T55	54-55	HYPH	denotes	-
T54	55-63	VBN	denotes	Targeted
T56	64-68	NNS	denotes	Mice
T57	68-255	sentence	denotes	MCAD Gene Disruption in the Mouse Abstract Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency is the most common inherited disorder of mitochondrial fatty acid β-oxidation in humans.
T58	69-119	JJ	denotes	MCAD Gene Disruption in the Mouse Abstract Medium
T60	119-120	HYPH	denotes	-
T59	120-125	NN	denotes	chain
T62	126-130	NN	denotes	acyl
T64	130-131	HYPH	denotes	-
T63	131-134	NN	denotes	CoA
T61	135-148	NN	denotes	dehydrogenase
T66	149-150	-LRB-	denotes	(
T67	150-154	NN	denotes	MCAD
T68	154-155	-RRB-	denotes	)
T65	156-166	NN	denotes	deficiency
T69	167-169	VBZ	denotes	is
T70	170-173	DT	denotes	the
T72	174-178	RBS	denotes	most
T73	179-185	JJ	denotes	common
T74	186-195	VBN	denotes	inherited
T71	196-204	NN	denotes	disorder
T75	205-207	IN	denotes	of
T76	208-221	JJ	denotes	mitochondrial
T78	222-227	JJ	denotes	fatty
T79	228-232	NN	denotes	acid
T80	233-234	NN	denotes	β
T81	234-235	HYPH	denotes	-
T77	235-244	NN	denotes	oxidation
T82	245-247	IN	denotes	in
T83	248-254	NNS	denotes	humans
T84	254-255	.	denotes	.
T85	255-415	sentence	denotes	To better understand the pathogenesis of this disease, we developed a mouse model for MCAD deficiency (MCAD−/−) by gene targeting in embryonic stem (ES) cells.
T86	256-258	TO	denotes	To
T88	259-265	RBR	denotes	better
T87	266-276	VB	denotes	understand
T90	277-280	DT	denotes	the
T91	281-293	NN	denotes	pathogenesis
T92	294-296	IN	denotes	of
T93	297-301	DT	denotes	this
T94	302-309	NN	denotes	disease
T95	309-311	,	denotes	,
T96	311-313	PRP	denotes	we
T89	314-323	VBD	denotes	developed
T97	324-325	DT	denotes	a
T99	326-331	NN	denotes	mouse
T98	332-337	NN	denotes	model
T100	338-341	IN	denotes	for
T101	342-346	NN	denotes	MCAD
T102	347-357	NN	denotes	deficiency
T103	358-359	-LRB-	denotes	(
T104	359-363	NN	denotes	MCAD
T105	363-364	SYM	denotes	−
T106	364-365	HYPH	denotes	/
T107	365-366	SYM	denotes	−
T108	366-367	-RRB-	denotes	)
T109	368-370	IN	denotes	by
T110	371-375	NN	denotes	gene
T111	376-385	NN	denotes	targeting
T112	386-388	IN	denotes	in
T113	389-398	JJ	denotes	embryonic
T114	399-403	NN	denotes	stem
T116	404-405	-LRB-	denotes	(
T117	405-407	NN	denotes	ES
T118	407-408	-RRB-	denotes	)
T115	409-414	NNS	denotes	cells
T119	414-415	.	denotes	.
T120	415-544	sentence	denotes	The MCAD−/− mice developed an organic aciduria and fatty liver, and showed profound cold intolerance at 4 °C with prior fasting.
T121	416-419	DT	denotes	The
T123	420-424	NN	denotes	MCAD
T124	424-425	SYM	denotes	−
T125	425-426	HYPH	denotes	/
T126	426-427	SYM	denotes	−
T122	428-432	NNS	denotes	mice
T127	433-442	VBD	denotes	developed
T128	443-445	DT	denotes	an
T130	446-453	JJ	denotes	organic
T129	454-462	NN	denotes	aciduria
T131	463-466	CC	denotes	and
T132	467-472	JJ	denotes	fatty
T133	473-478	NN	denotes	liver
T134	478-480	,	denotes	,
T135	480-483	CC	denotes	and
T136	484-490	VBD	denotes	showed
T137	491-499	JJ	denotes	profound
T139	500-504	NN	denotes	cold
T138	505-516	NN	denotes	intolerance
T140	517-519	IN	denotes	at
T141	520-521	CD	denotes	4
T142	522-524	NN	denotes	°C
T143	525-529	IN	denotes	with
T144	530-535	JJ	denotes	prior
T145	536-543	NN	denotes	fasting
T146	543-544	.	denotes	.
T147	544-641	sentence	denotes	The sporadic cardiac lesions seen in MCAD−/− mice have not been reported in human MCAD patients.
T148	545-548	DT	denotes	The
T150	549-557	JJ	denotes	sporadic
T151	558-565	JJ	denotes	cardiac
T149	566-573	NNS	denotes	lesions
T153	574-578	VBN	denotes	seen
T154	579-581	IN	denotes	in
T155	582-586	NN	denotes	MCAD
T157	586-587	SYM	denotes	−
T158	587-588	HYPH	denotes	/
T159	588-589	SYM	denotes	−
T156	590-594	NNS	denotes	mice
T160	595-599	VBP	denotes	have
T161	600-603	RB	denotes	not
T162	604-608	VBN	denotes	been
T152	609-617	VBN	denotes	reported
T163	618-620	IN	denotes	in
T164	621-626	JJ	denotes	human
T166	627-631	NN	denotes	MCAD
T165	632-640	NNS	denotes	patients
T167	640-641	.	denotes	.
T168	641-800	sentence	denotes	There was significant neonatal mortality of MCAD−/− pups demonstrating similarities to patterns of clinical episodes and mortality in MCAD-deficient patients.
T169	642-647	EX	denotes	There
T170	648-651	VBD	denotes	was
T171	652-663	JJ	denotes	significant
T173	664-672	JJ	denotes	neonatal
T172	673-682	NN	denotes	mortality
T174	683-685	IN	denotes	of
T175	686-690	NN	denotes	MCAD
T177	690-691	SYM	denotes	−
T178	691-692	HYPH	denotes	/
T179	692-693	SYM	denotes	−
T176	694-698	NNS	denotes	pups
T180	699-712	VBG	denotes	demonstrating
T181	713-725	NNS	denotes	similarities
T182	726-728	IN	denotes	to
T183	729-737	NNS	denotes	patterns
T184	738-740	IN	denotes	of
T185	741-749	JJ	denotes	clinical
T186	750-758	NNS	denotes	episodes
T187	759-762	CC	denotes	and
T188	763-772	NN	denotes	mortality
T189	773-775	IN	denotes	in
T190	776-780	NN	denotes	MCAD
T192	780-781	HYPH	denotes	-
T191	781-790	JJ	denotes	deficient
T193	791-799	NNS	denotes	patients
T194	799-800	.	denotes	.
T195	800-1028	sentence	denotes	The MCAD-deficient mouse reproduced important aspects of human MCAD deficiency and is a valuable model for further analysis of the roles of fatty acid oxidation and pathogenesis of human diseases involving fatty acid oxidation.
T196	801-804	DT	denotes	The
T198	805-809	NN	denotes	MCAD
T200	809-810	HYPH	denotes	-
T199	810-819	JJ	denotes	deficient
T197	820-825	NN	denotes	mouse
T201	826-836	VBD	denotes	reproduced
T202	837-846	JJ	denotes	important
T203	847-854	NNS	denotes	aspects
T204	855-857	IN	denotes	of
T205	858-863	JJ	denotes	human
T207	864-868	NN	denotes	MCAD
T206	869-879	NN	denotes	deficiency
T208	880-883	CC	denotes	and
T209	884-886	VBZ	denotes	is
T210	887-888	DT	denotes	a
T212	889-897	JJ	denotes	valuable
T211	898-903	NN	denotes	model
T213	904-907	IN	denotes	for
T214	908-915	JJ	denotes	further
T215	916-924	NN	denotes	analysis
T216	925-927	IN	denotes	of
T217	928-931	DT	denotes	the
T218	932-937	NNS	denotes	roles
T219	938-940	IN	denotes	of
T220	941-946	JJ	denotes	fatty
T221	947-951	NN	denotes	acid
T222	952-961	NN	denotes	oxidation
T223	962-965	CC	denotes	and
T224	966-978	NN	denotes	pathogenesis
T225	979-981	IN	denotes	of
T226	982-987	JJ	denotes	human
T227	988-996	NNS	denotes	diseases
T228	997-1006	VBG	denotes	involving
T229	1007-1012	JJ	denotes	fatty
T230	1013-1017	NN	denotes	acid
T231	1018-1027	NN	denotes	oxidation
T232	1027-1028	.	denotes	.
T373	2143-2156	JJ	denotes	Mitochondrial
T375	2157-2158	NN	denotes	β
T376	2158-2159	HYPH	denotes	-
T374	2159-2168	NN	denotes	oxidation
T378	2169-2171	IN	denotes	of
T379	2172-2177	JJ	denotes	fatty
T380	2178-2183	NNS	denotes	acids
T377	2184-2192	VBZ	denotes	provides
T381	2193-2199	NN	denotes	energy
T382	2199-2201	,	denotes	,
T383	2201-2211	RB	denotes	especially
T384	2212-2218	IN	denotes	during
T385	2219-2226	NN	denotes	fasting
T386	2227-2237	NNS	denotes	conditions
T387	2237-2238	.	denotes	.
T388	2238-2344	sentence	denotes	Fatty acid oxidation occurs in mitochondria and consists of a repeating circuit of four sequential steps.
T389	2239-2244	JJ	denotes	Fatty
T390	2245-2249	NN	denotes	acid
T391	2250-2259	NN	denotes	oxidation
T392	2260-2266	VBZ	denotes	occurs
T393	2267-2269	IN	denotes	in
T394	2270-2282	NNS	denotes	mitochondria
T395	2283-2286	CC	denotes	and
T396	2287-2295	VBZ	denotes	consists
T397	2296-2298	IN	denotes	of
T398	2299-2300	DT	denotes	a
T400	2301-2310	VBG	denotes	repeating
T399	2311-2318	NN	denotes	circuit
T401	2319-2321	IN	denotes	of
T402	2322-2326	CD	denotes	four
T404	2327-2337	JJ	denotes	sequential
T403	2338-2343	NNS	denotes	steps
T405	2343-2344	.	denotes	.
T406	2344-2428	sentence	denotes	There are four straight-chain acyl-CoA dehydrogenases involved in the initial step.
T407	2345-2350	EX	denotes	There
T408	2351-2354	VBP	denotes	are
T409	2355-2359	CD	denotes	four
T411	2360-2368	JJ	denotes	straight
T413	2368-2369	HYPH	denotes	-
T412	2369-2374	NN	denotes	chain
T414	2375-2379	NN	denotes	acyl
T416	2379-2380	HYPH	denotes	-
T415	2380-2383	NN	denotes	CoA
T410	2384-2398	NNS	denotes	dehydrogenases
T417	2399-2407	VBN	denotes	involved
T418	2408-2410	IN	denotes	in
T419	2411-2414	DT	denotes	the
T421	2415-2422	JJ	denotes	initial
T420	2423-2427	NN	denotes	step
T422	2427-2428	.	denotes	.
T423	2428-2650	sentence	denotes	Medium-chain acyl-CoA dehydrogenase (MCAD) (the mouse gene is Acadm, whereas the protein is MCAD), specifically, is responsible for catalyzing the dehydrogenation of medium-chain length (C6–C12) fatty acid thioesters [1].
T424	2429-2435	JJ	denotes	Medium
T426	2435-2436	HYPH	denotes	-
T425	2436-2441	NN	denotes	chain
T428	2442-2446	NN	denotes	acyl
T430	2446-2447	HYPH	denotes	-
T429	2447-2450	NN	denotes	CoA
T427	2451-2464	NN	denotes	dehydrogenase
T432	2465-2466	-LRB-	denotes	(
T433	2466-2470	NN	denotes	MCAD
T434	2470-2471	-RRB-	denotes	)
T435	2472-2473	-LRB-	denotes	(
T437	2473-2476	DT	denotes	the
T439	2477-2482	NN	denotes	mouse
T438	2483-2487	NN	denotes	gene
T436	2488-2490	VBZ	denotes	is
T440	2491-2496	NN	denotes	Acadm
T441	2496-2498	,	denotes	,
T442	2498-2505	IN	denotes	whereas
T444	2506-2509	DT	denotes	the
T445	2510-2517	NN	denotes	protein
T443	2518-2520	VBZ	denotes	is
T446	2521-2525	NN	denotes	MCAD
T447	2525-2526	-RRB-	denotes	)
T448	2526-2528	,	denotes	,
T449	2528-2540	RB	denotes	specifically
T450	2540-2542	,	denotes	,
T431	2542-2544	VBZ	denotes	is
T451	2545-2556	JJ	denotes	responsible
T452	2557-2560	IN	denotes	for
T453	2561-2571	VBG	denotes	catalyzing
T454	2572-2575	DT	denotes	the
T455	2576-2591	NN	denotes	dehydrogenation
T456	2592-2594	IN	denotes	of
T457	2595-2601	JJ	denotes	medium
T459	2601-2602	HYPH	denotes	-
T458	2602-2607	NN	denotes	chain
T460	2608-2614	NN	denotes	length
T462	2615-2616	-LRB-	denotes	(
T464	2616-2618	NN	denotes	C6
T465	2618-2619	SYM	denotes	–
T463	2619-2622	NN	denotes	C12
T466	2622-2623	-RRB-	denotes	)
T467	2624-2629	JJ	denotes	fatty
T468	2630-2634	NN	denotes	acid
T461	2635-2645	NNS	denotes	thioesters
T469	2646-2647	-LRB-	denotes	[
T470	2647-2648	CD	denotes	1
T471	2648-2649	-RRB-	denotes	]
T472	2649-2650	.	denotes	.
T473	2650-2768	sentence	denotes	Acadm is transcribed in the nucleus, translated in the cytosol, and translocated into the mitochondrial matrix [2–4].
T474	2651-2656	NN	denotes	Acadm
T476	2657-2659	VBZ	denotes	is
T475	2660-2671	VBN	denotes	transcribed
T477	2672-2674	IN	denotes	in
T478	2675-2678	DT	denotes	the
T479	2679-2686	NN	denotes	nucleus
T480	2686-2688	,	denotes	,
T481	2688-2698	VBN	denotes	translated
T482	2699-2701	IN	denotes	in
T483	2702-2705	DT	denotes	the
T484	2706-2713	NN	denotes	cytosol
T485	2713-2715	,	denotes	,
T486	2715-2718	CC	denotes	and
T487	2719-2731	VBN	denotes	translocated
T488	2732-2736	IN	denotes	into
T489	2737-2740	DT	denotes	the
T491	2741-2754	JJ	denotes	mitochondrial
T490	2755-2761	NN	denotes	matrix
T492	2762-2763	-LRB-	denotes	[
T493	2763-2764	CD	denotes	2
T494	2764-2765	SYM	denotes	–
T495	2765-2766	CD	denotes	4
T496	2766-2767	-RRB-	denotes	]
T497	2767-2768	.	denotes	.
T498	2768-2889	sentence	denotes	Once inside the mitochondrial matrix, the MCAD monomers are assembled into homotetramers to gain enzymatic activity [4].
T499	2769-2773	IN	denotes	Once
T500	2774-2780	IN	denotes	inside
T502	2781-2784	DT	denotes	the
T504	2785-2798	JJ	denotes	mitochondrial
T503	2799-2805	NN	denotes	matrix
T505	2805-2807	,	denotes	,
T506	2807-2810	DT	denotes	the
T508	2811-2815	NN	denotes	MCAD
T507	2816-2824	NNS	denotes	monomers
T509	2825-2828	VBP	denotes	are
T501	2829-2838	VBN	denotes	assembled
T510	2839-2843	IN	denotes	into
T511	2844-2857	NNS	denotes	homotetramers
T512	2858-2860	TO	denotes	to
T513	2861-2865	VB	denotes	gain
T514	2866-2875	JJ	denotes	enzymatic
T515	2876-2884	NN	denotes	activity
T516	2885-2886	-LRB-	denotes	[
T517	2886-2887	CD	denotes	4
T518	2887-2888	-RRB-	denotes	]
T519	2888-2889	.	denotes	.
T520	2889-2951	sentence	denotes	MCAD activity is essential for complete fatty acid oxidation.
T521	2890-2894	NN	denotes	MCAD
T522	2895-2903	NN	denotes	activity
T523	2904-2906	VBZ	denotes	is
T524	2907-2916	JJ	denotes	essential
T525	2917-2920	IN	denotes	for
T526	2921-2929	JJ	denotes	complete
T528	2930-2935	JJ	denotes	fatty
T529	2936-2940	NN	denotes	acid
T527	2941-2950	NN	denotes	oxidation
T530	2950-2951	.	denotes	.
T531	2951-3030	sentence	denotes	Inherited MCAD deficiency exists in humans as an autosomal recessive disorder.
T532	2952-2961	VBN	denotes	Inherited
T534	2962-2966	NN	denotes	MCAD
T533	2967-2977	NN	denotes	deficiency
T535	2978-2984	VBZ	denotes	exists
T536	2985-2987	IN	denotes	in
T537	2988-2994	NNS	denotes	humans
T538	2995-2997	IN	denotes	as
T539	2998-3000	DT	denotes	an
T541	3001-3010	JJ	denotes	autosomal
T542	3011-3020	JJ	denotes	recessive
T540	3021-3029	NN	denotes	disorder
T543	3029-3030	.	denotes	.
T544	3030-3139	sentence	denotes	MCAD deficiency was first described in 1982—1983 [5–7] and has been described in numerous patients [1,8–11].
T545	3031-3035	NN	denotes	MCAD
T546	3036-3046	NN	denotes	deficiency
T548	3047-3050	VBD	denotes	was
T549	3051-3056	RB	denotes	first
T547	3057-3066	VBN	denotes	described
T550	3067-3069	IN	denotes	in
T551	3070-3074	CD	denotes	1982
T552	3074-3075	SYM	denotes	—
T553	3075-3079	CD	denotes	1983
T554	3080-3081	-LRB-	denotes	[
T555	3081-3082	CD	denotes	5
T556	3082-3083	SYM	denotes	–
T557	3083-3084	CD	denotes	7
T558	3084-3085	-RRB-	denotes	]
T559	3086-3089	CC	denotes	and
T560	3090-3093	VBZ	denotes	has
T562	3094-3098	VBN	denotes	been
T561	3099-3108	VBN	denotes	described
T563	3109-3111	IN	denotes	in
T564	3112-3120	JJ	denotes	numerous
T565	3121-3129	NNS	denotes	patients
T566	3130-3131	-LRB-	denotes	[
T567	3131-3132	CD	denotes	1
T568	3132-3133	,	denotes	,
T569	3133-3134	CD	denotes	8
T570	3134-3135	SYM	denotes	–
T571	3135-3137	CD	denotes	11
T572	3137-3138	-RRB-	denotes	]
T573	3138-3139	.	denotes	.
T574	3139-3315	sentence	denotes	The carrier frequency in the Caucasian population has been estimated to be between 1 in 50 to 80 with an incidence of clinical disease expected at around 1 in 15,000 [1,9,12].
T575	3140-3143	DT	denotes	The
T577	3144-3151	NN	denotes	carrier
T576	3152-3161	NN	denotes	frequency
T579	3162-3164	IN	denotes	in
T580	3165-3168	DT	denotes	the
T582	3169-3178	JJ	denotes	Caucasian
T581	3179-3189	NN	denotes	population
T583	3190-3193	VBZ	denotes	has
T584	3194-3198	VBN	denotes	been
T578	3199-3208	VBN	denotes	estimated
T585	3209-3211	TO	denotes	to
T586	3212-3214	VB	denotes	be
T587	3215-3222	IN	denotes	between
T589	3223-3224	CD	denotes	1
T590	3225-3227	IN	denotes	in
T591	3228-3230	CD	denotes	50
T592	3231-3233	IN	denotes	to
T588	3234-3236	CD	denotes	80
T593	3237-3241	IN	denotes	with
T594	3242-3244	DT	denotes	an
T595	3245-3254	NN	denotes	incidence
T596	3255-3257	IN	denotes	of
T597	3258-3266	JJ	denotes	clinical
T598	3267-3274	NN	denotes	disease
T599	3275-3283	VBN	denotes	expected
T600	3284-3286	IN	denotes	at
T601	3287-3293	IN	denotes	around
T602	3294-3295	CD	denotes	1
T604	3296-3298	IN	denotes	in
T603	3299-3305	CD	denotes	15,000
T605	3306-3307	-LRB-	denotes	[
T607	3307-3308	CD	denotes	1
T608	3308-3309	,	denotes	,
T609	3309-3310	CD	denotes	9
T610	3310-3311	,	denotes	,
T606	3311-3313	CD	denotes	12
T611	3313-3314	-RRB-	denotes	]
T612	3314-3315	.	denotes	.
T613	3315-3396	sentence	denotes	MCAD-deficient patients exhibit clinical episodes often associated with fasting.
T614	3316-3320	NN	denotes	MCAD
T616	3320-3321	HYPH	denotes	-
T615	3321-3330	JJ	denotes	deficient
T617	3331-3339	NNS	denotes	patients
T618	3340-3347	VBP	denotes	exhibit
T619	3348-3356	JJ	denotes	clinical
T620	3357-3365	NNS	denotes	episodes
T621	3366-3371	RB	denotes	often
T622	3372-3382	VBN	denotes	associated
T623	3383-3387	IN	denotes	with
T624	3388-3395	NN	denotes	fasting
T625	3395-3396	.	denotes	.
T626	3396-3466	sentence	denotes	Patients manifest disease usually during the first two years of life.
T627	3397-3405	NNS	denotes	Patients
T628	3406-3414	VBP	denotes	manifest
T629	3415-3422	NN	denotes	disease
T630	3423-3430	RB	denotes	usually
T631	3431-3437	IN	denotes	during
T632	3438-3441	DT	denotes	the
T634	3442-3447	JJ	denotes	first
T635	3448-3451	CD	denotes	two
T633	3452-3457	NNS	denotes	years
T636	3458-3460	IN	denotes	of
T637	3461-3465	NN	denotes	life
T638	3465-3466	.	denotes	.
T639	3466-3536	sentence	denotes	Symptoms include hypoketotic hypoglycemia and Reye-like episodes [1].
T640	3467-3475	NNS	denotes	Symptoms
T641	3476-3483	VBP	denotes	include
T642	3484-3495	JJ	denotes	hypoketotic
T643	3496-3508	NN	denotes	hypoglycemia
T644	3509-3512	CC	denotes	and
T645	3513-3517	NN	denotes	Reye
T647	3517-3518	HYPH	denotes	-
T646	3518-3522	JJ	denotes	like
T648	3523-3531	NNS	denotes	episodes
T649	3532-3533	-LRB-	denotes	[
T650	3533-3534	CD	denotes	1
T651	3534-3535	-RRB-	denotes	]
T652	3535-3536	.	denotes	.
T653	3536-3681	sentence	denotes	It is estimated that approximately 59% of patients presenting clinically between 15 to 26 mo of age die during their first clinical episode [1].
T654	3537-3539	PRP	denotes	It
T656	3540-3542	VBZ	denotes	is
T655	3543-3552	VBN	denotes	estimated
T657	3553-3557	IN	denotes	that
T659	3558-3571	RB	denotes	approximately
T660	3572-3574	CD	denotes	59
T661	3574-3575	NN	denotes	%
T662	3576-3578	IN	denotes	of
T663	3579-3587	NNS	denotes	patients
T664	3588-3598	VBG	denotes	presenting
T665	3599-3609	RB	denotes	clinically
T666	3610-3617	IN	denotes	between
T667	3618-3620	CD	denotes	15
T669	3621-3623	IN	denotes	to
T668	3624-3626	CD	denotes	26
T670	3627-3629	NN	denotes	mo
T671	3630-3632	IN	denotes	of
T672	3633-3636	NN	denotes	age
T658	3637-3640	VBP	denotes	die
T673	3641-3647	IN	denotes	during
T674	3648-3653	PRP$	denotes	their
T676	3654-3659	JJ	denotes	first
T677	3660-3668	JJ	denotes	clinical
T675	3669-3676	NN	denotes	episode
T678	3677-3678	-LRB-	denotes	[
T679	3678-3679	CD	denotes	1
T680	3679-3680	-RRB-	denotes	]
T681	3680-3681	.	denotes	.
T682	3681-3843	sentence	denotes	The pathogenesis of the wide range of metabolic disturbances in MCAD deficiency is poorly understood and certain aspects of patient management are controversial.
T683	3682-3685	DT	denotes	The
T684	3686-3698	NN	denotes	pathogenesis
T686	3699-3701	IN	denotes	of
T687	3702-3705	DT	denotes	the
T689	3706-3710	JJ	denotes	wide
T688	3711-3716	NN	denotes	range
T690	3717-3719	IN	denotes	of
T691	3720-3729	JJ	denotes	metabolic
T692	3730-3742	NNS	denotes	disturbances
T693	3743-3745	IN	denotes	in
T694	3746-3750	NN	denotes	MCAD
T695	3751-3761	NN	denotes	deficiency
T696	3762-3764	VBZ	denotes	is
T697	3765-3771	RB	denotes	poorly
T685	3772-3782	VBN	denotes	understood
T698	3783-3786	CC	denotes	and
T699	3787-3794	JJ	denotes	certain
T700	3795-3802	NNS	denotes	aspects
T702	3803-3805	IN	denotes	of
T703	3806-3813	NN	denotes	patient
T704	3814-3824	NN	denotes	management
T701	3825-3828	VBP	denotes	are
T705	3829-3842	JJ	denotes	controversial
T706	3842-3843	.	denotes	.
T707	3843-4011	sentence	denotes	An animal model for MCAD deficiency is essential to better understand the pathogenesis of MCAD deficiency and to develop better management regimens for human patients.
T708	3844-3846	DT	denotes	An
T710	3847-3853	NN	denotes	animal
T709	3854-3859	NN	denotes	model
T712	3860-3863	IN	denotes	for
T713	3864-3868	NN	denotes	MCAD
T714	3869-3879	NN	denotes	deficiency
T711	3880-3882	VBZ	denotes	is
T715	3883-3892	JJ	denotes	essential
T716	3893-3895	TO	denotes	to
T718	3896-3902	RBR	denotes	better
T717	3903-3913	VB	denotes	understand
T719	3914-3917	DT	denotes	the
T720	3918-3930	NN	denotes	pathogenesis
T721	3931-3933	IN	denotes	of
T722	3934-3938	NN	denotes	MCAD
T723	3939-3949	NN	denotes	deficiency
T724	3950-3953	CC	denotes	and
T725	3954-3956	TO	denotes	to
T726	3957-3964	VB	denotes	develop
T727	3965-3971	JJR	denotes	better
T729	3972-3982	NN	denotes	management
T728	3983-3991	NNS	denotes	regimens
T730	3992-3995	IN	denotes	for
T731	3996-4001	JJ	denotes	human
T732	4002-4010	NNS	denotes	patients
T733	4010-4011	.	denotes	.
T734	4011-4188	sentence	denotes	To gain further insight into the mechanisms of this disease, we developed a mouse model of MCAD deficiency by gene targeting in embryonic stem (ES) cells (for reviews [13,14]).
T735	4012-4014	TO	denotes	To
T736	4015-4019	VB	denotes	gain
T738	4020-4027	JJ	denotes	further
T739	4028-4035	NN	denotes	insight
T740	4036-4040	IN	denotes	into
T741	4041-4044	DT	denotes	the
T742	4045-4055	NNS	denotes	mechanisms
T743	4056-4058	IN	denotes	of
T744	4059-4063	DT	denotes	this
T745	4064-4071	NN	denotes	disease
T746	4071-4073	,	denotes	,
T747	4073-4075	PRP	denotes	we
T737	4076-4085	VBD	denotes	developed
T748	4086-4087	DT	denotes	a
T750	4088-4093	NN	denotes	mouse
T749	4094-4099	NN	denotes	model
T751	4100-4102	IN	denotes	of
T752	4103-4107	NN	denotes	MCAD
T753	4108-4118	NN	denotes	deficiency
T754	4119-4121	IN	denotes	by
T755	4122-4126	NN	denotes	gene
T756	4127-4136	NN	denotes	targeting
T757	4137-4139	IN	denotes	in
T758	4140-4149	JJ	denotes	embryonic
T759	4150-4154	NN	denotes	stem
T761	4155-4156	-LRB-	denotes	(
T762	4156-4158	NN	denotes	ES
T763	4158-4159	-RRB-	denotes	)
T760	4160-4165	NNS	denotes	cells
T764	4166-4167	-LRB-	denotes	(
T766	4167-4170	IN	denotes	for
T767	4171-4178	NNS	denotes	reviews
T768	4179-4180	-LRB-	denotes	[
T769	4180-4182	CD	denotes	13
T770	4182-4183	,	denotes	,
T765	4183-4185	CD	denotes	14
T771	4185-4186	-RRB-	denotes	]
T772	4186-4187	-RRB-	denotes	)
T773	4187-4188	.	denotes	.
T774	4188-4338	sentence	denotes	The mutant mice had many relevant features characteristic of the disease found in human MCAD-deficient patients, along with some unexpected findings.
T775	4189-4192	DT	denotes	The
T777	4193-4199	NN	denotes	mutant
T776	4200-4204	NNS	denotes	mice
T778	4205-4208	VBD	denotes	had
T779	4209-4213	JJ	denotes	many
T781	4214-4222	JJ	denotes	relevant
T780	4223-4231	NNS	denotes	features
T782	4232-4246	JJ	denotes	characteristic
T783	4247-4249	IN	denotes	of
T784	4250-4253	DT	denotes	the
T785	4254-4261	NN	denotes	disease
T786	4262-4267	VBN	denotes	found
T787	4268-4270	IN	denotes	in
T788	4271-4276	JJ	denotes	human
T790	4277-4281	NN	denotes	MCAD
T792	4281-4282	HYPH	denotes	-
T791	4282-4291	JJ	denotes	deficient
T789	4292-4300	NNS	denotes	patients
T793	4300-4302	,	denotes	,
T794	4302-4307	IN	denotes	along
T795	4308-4312	IN	denotes	with
T796	4313-4317	DT	denotes	some
T798	4318-4328	JJ	denotes	unexpected
T797	4329-4337	NNS	denotes	findings
T799	4337-4338	.	denotes	.
T964	4349-4353	NN	denotes	Gene
T965	4354-4363	NN	denotes	Targeting
T966	4364-4367	CC	denotes	and
T967	4368-4378	NN	denotes	Generation
T968	4379-4381	IN	denotes	of
T969	4382-4386	NN	denotes	MCAD
T971	4386-4387	HYPH	denotes	-
T970	4387-4396	JJ	denotes	Deficient
T972	4397-4401	NNS	denotes	Mice
T973	4401-4568	sentence	denotes	MCAD insertion vector (MCAD IV2) was designed to undergo gap repair of the 1.3-kb deleted region upon homologous recombination in 129P2 (129P2/OlaHsd) ES cells E14–1.
T974	4402-4406	NN	denotes	MCAD
T976	4407-4416	NN	denotes	insertion
T975	4417-4423	NN	denotes	vector
T978	4424-4425	-LRB-	denotes	(
T979	4425-4429	NN	denotes	MCAD
T980	4430-4433	NN	denotes	IV2
T981	4433-4434	-RRB-	denotes	)
T982	4435-4438	VBD	denotes	was
T977	4439-4447	VBN	denotes	designed
T983	4448-4450	TO	denotes	to
T984	4451-4458	VB	denotes	undergo
T985	4459-4462	NN	denotes	gap
T986	4463-4469	NN	denotes	repair
T987	4470-4472	IN	denotes	of
T988	4473-4476	DT	denotes	the
T990	4477-4480	CD	denotes	1.3
T992	4480-4481	HYPH	denotes	-
T991	4481-4483	NN	denotes	kb
T993	4484-4491	VBN	denotes	deleted
T989	4492-4498	NN	denotes	region
T994	4499-4503	IN	denotes	upon
T995	4504-4514	JJ	denotes	homologous
T996	4515-4528	NN	denotes	recombination
T997	4529-4531	IN	denotes	in
T998	4532-4537	NN	denotes	129P2
T1000	4538-4539	-LRB-	denotes	(
T1002	4539-4544	NN	denotes	129P2
T1003	4544-4545	HYPH	denotes	/
T1001	4545-4551	NN	denotes	OlaHsd
T1004	4551-4552	-RRB-	denotes	)
T1005	4553-4555	NN	denotes	ES
T999	4556-4561	NNS	denotes	cells
T1006	4562-4565	NN	denotes	E14
T1007	4565-4566	HYPH	denotes	–
T1008	4566-4567	CD	denotes	1
T1009	4567-4568	.	denotes	.
T1010	4568-4719	sentence	denotes	Correct targeting of the MCAD locus resulted in a duplication of exons 8, 9, and 10 and integration of flanking plasmid and Neo sequences (Figure 1A).
T1011	4569-4576	JJ	denotes	Correct
T1012	4577-4586	NN	denotes	targeting
T1014	4587-4589	IN	denotes	of
T1015	4590-4593	DT	denotes	the
T1017	4594-4598	NN	denotes	MCAD
T1016	4599-4604	NN	denotes	locus
T1013	4605-4613	VBD	denotes	resulted
T1018	4614-4616	IN	denotes	in
T1019	4617-4618	DT	denotes	a
T1020	4619-4630	NN	denotes	duplication
T1021	4631-4633	IN	denotes	of
T1022	4634-4639	NNS	denotes	exons
T1023	4640-4641	CD	denotes	8
T1024	4641-4643	,	denotes	,
T1025	4643-4644	CD	denotes	9
T1026	4644-4646	,	denotes	,
T1027	4646-4649	CC	denotes	and
T1028	4650-4652	CD	denotes	10
T1029	4653-4656	CC	denotes	and
T1030	4657-4668	NN	denotes	integration
T1031	4669-4671	IN	denotes	of
T1032	4672-4680	VBG	denotes	flanking
T1033	4681-4688	NN	denotes	plasmid
T1034	4689-4692	CC	denotes	and
T1035	4693-4696	NN	denotes	Neo
T1036	4697-4706	NNS	denotes	sequences
T1037	4707-4708	-LRB-	denotes	(
T1038	4708-4714	NN	denotes	Figure
T1039	4715-4717	CD	denotes	1A
T1040	4717-4718	-RRB-	denotes	)
T1041	4718-4719	.	denotes	.
T1042	4719-4803	sentence	denotes	The insertion vector was designed to duplicate exon 8, 9, and 10 at the MCAD locus.
T1043	4720-4723	DT	denotes	The
T1045	4724-4733	NN	denotes	insertion
T1044	4734-4740	NN	denotes	vector
T1047	4741-4744	VBD	denotes	was
T1046	4745-4753	VBN	denotes	designed
T1048	4754-4756	TO	denotes	to
T1049	4757-4766	VB	denotes	duplicate
T1050	4767-4771	NN	denotes	exon
T1051	4772-4773	CD	denotes	8
T1052	4773-4775	,	denotes	,
T1053	4775-4776	CD	denotes	9
T1054	4776-4778	,	denotes	,
T1055	4778-4781	CC	denotes	and
T1056	4782-4784	CD	denotes	10
T1057	4785-4787	IN	denotes	at
T1058	4788-4791	DT	denotes	the
T1060	4792-4796	NN	denotes	MCAD
T1059	4797-4802	NN	denotes	locus
T1061	4802-4803	.	denotes	.
T1062	4803-4942	sentence	denotes	Translation of the duplicated exon 8 region results in the formation of premature stop codons resulting in truncation of the MCAD monomer.
T1063	4804-4815	NN	denotes	Translation
T1065	4816-4818	IN	denotes	of
T1066	4819-4822	DT	denotes	the
T1068	4823-4833	VBN	denotes	duplicated
T1069	4834-4838	NN	denotes	exon
T1070	4839-4840	CD	denotes	8
T1067	4841-4847	NN	denotes	region
T1064	4848-4855	VBZ	denotes	results
T1071	4856-4858	IN	denotes	in
T1072	4859-4862	DT	denotes	the
T1073	4863-4872	NN	denotes	formation
T1074	4873-4875	IN	denotes	of
T1075	4876-4885	JJ	denotes	premature
T1077	4886-4890	NN	denotes	stop
T1076	4891-4897	NNS	denotes	codons
T1078	4898-4907	VBG	denotes	resulting
T1079	4908-4910	IN	denotes	in
T1080	4911-4921	NN	denotes	truncation
T1081	4922-4924	IN	denotes	of
T1082	4925-4928	DT	denotes	the
T1084	4929-4933	NN	denotes	MCAD
T1083	4934-4941	NN	denotes	monomer
T1085	4941-4942	.	denotes	.
T1086	4942-5066	sentence	denotes	Specifically, the first premature stop codon arises after translation of only seven amino acids from the duplicated exon 8.
T1087	4943-4955	RB	denotes	Specifically
T1089	4955-4957	,	denotes	,
T1090	4957-4960	DT	denotes	the
T1092	4961-4966	JJ	denotes	first
T1093	4967-4976	JJ	denotes	premature
T1094	4977-4981	NN	denotes	stop
T1091	4982-4987	NN	denotes	codon
T1088	4988-4994	VBZ	denotes	arises
T1095	4995-5000	IN	denotes	after
T1096	5001-5012	NN	denotes	translation
T1097	5013-5015	IN	denotes	of
T1098	5016-5020	RB	denotes	only
T1099	5021-5026	CD	denotes	seven
T1101	5027-5032	NN	denotes	amino
T1100	5033-5038	NNS	denotes	acids
T1102	5039-5043	IN	denotes	from
T1103	5044-5047	DT	denotes	the
T1105	5048-5058	VBN	denotes	duplicated
T1104	5059-5063	NN	denotes	exon
T1106	5064-5065	CD	denotes	8
T1107	5065-5066	.	denotes	.
T1108	5066-5236	sentence	denotes	The resulting MCAD monomer is missing the C-terminal domain α-helixes that are responsible for making intersubunit contacts to generate the functional MCAD homotetramer.
T1109	5067-5070	DT	denotes	The
T1111	5071-5080	VBG	denotes	resulting
T1112	5081-5085	NN	denotes	MCAD
T1110	5086-5093	NN	denotes	monomer
T1114	5094-5096	VBZ	denotes	is
T1113	5097-5104	VBG	denotes	missing
T1115	5105-5108	DT	denotes	the
T1117	5109-5110	NN	denotes	C
T1119	5110-5111	HYPH	denotes	-
T1118	5111-5119	JJ	denotes	terminal
T1120	5120-5126	NN	denotes	domain
T1121	5127-5128	NN	denotes	α
T1122	5128-5129	HYPH	denotes	-
T1116	5129-5136	NNS	denotes	helixes
T1123	5137-5141	WDT	denotes	that
T1124	5142-5145	VBP	denotes	are
T1125	5146-5157	JJ	denotes	responsible
T1126	5158-5161	IN	denotes	for
T1127	5162-5168	VBG	denotes	making
T1128	5169-5181	NN	denotes	intersubunit
T1129	5182-5190	NNS	denotes	contacts
T1130	5191-5193	TO	denotes	to
T1131	5194-5202	VB	denotes	generate
T1132	5203-5206	DT	denotes	the
T1134	5207-5217	JJ	denotes	functional
T1135	5218-5222	NN	denotes	MCAD
T1133	5223-5235	NN	denotes	homotetramer
T1136	5235-5236	.	denotes	.
T1137	5236-6060	sentence	denotes	Figure 1 Strategy for Disruption of the Mouse Acadm Gene (A) The MCAD IV2 insertion targeting vector with a deleted 1.3-kb region encompassing exon 10 and flanking sequences. MCAD IV2 undergoes gap repair upon homologous recombination at the endogenous Acadm locus resulting in a duplication of exons 8, 9, and 10 at the disrupted allele. (B) Southern blot analysis of EcoRI-digested genomic DNA from ES cells screened by PCR. Probe A, a DNA fragment consisting of a portion of exon 10 that is not present in the targeting vector, hybridizes to an endogenous 3.1-kb fragment and, upon homologous recombination, to a 13.2-kb fragment. Lane 1 represents a wild-type ES cell line, and Lane 2 and 3 represent targeted ES cell lines. ES cell clones were screened by PCR (data not shown) and confirmed by Southern blot analysis.
T7247	5247-5255	NN	denotes	Strategy
T7248	5256-5259	IN	denotes	for
T7249	5260-5270	NN	denotes	Disruption
T7250	5271-5273	IN	denotes	of
T7251	5274-5277	DT	denotes	the
T7253	5278-5283	NN	denotes	Mouse
T7254	5284-5289	NN	denotes	Acadm
T7252	5290-5294	NN	denotes	Gene
T7255	5294-5412	sentence	denotes	(A) The MCAD IV2 insertion targeting vector with a deleted 1.3-kb region encompassing exon 10 and flanking sequences.
T7256	5295-5296	-LRB-	denotes	(
T7257	5296-5297	LS	denotes	A
T7259	5297-5298	-RRB-	denotes	)
T7260	5299-5302	DT	denotes	The
T7261	5303-5307	NN	denotes	MCAD
T7263	5308-5311	NN	denotes	IV2
T7262	5312-5321	NN	denotes	insertion
T7264	5322-5331	NN	denotes	targeting
T7258	5332-5338	NN	denotes	vector
T7265	5339-5343	IN	denotes	with
T7266	5344-5345	DT	denotes	a
T7268	5346-5353	VBN	denotes	deleted
T7269	5354-5357	CD	denotes	1.3
T7271	5357-5358	HYPH	denotes	-
T7270	5358-5360	NN	denotes	kb
T7267	5361-5367	NN	denotes	region
T7272	5368-5380	VBG	denotes	encompassing
T7273	5381-5385	NN	denotes	exon
T7274	5386-5388	CD	denotes	10
T7275	5389-5392	CC	denotes	and
T7276	5393-5401	VBG	denotes	flanking
T7277	5402-5411	NNS	denotes	sequences
T7278	5411-5412	.	denotes	.
T7279	5412-5576	sentence	denotes	MCAD IV2 undergoes gap repair upon homologous recombination at the endogenous Acadm locus resulting in a duplication of exons 8, 9, and 10 at the disrupted allele.
T7280	5413-5417	NN	denotes	MCAD
T7281	5418-5421	NN	denotes	IV2
T7282	5422-5431	VBZ	denotes	undergoes
T7283	5432-5435	NN	denotes	gap
T7284	5436-5442	NN	denotes	repair
T7285	5443-5447	IN	denotes	upon
T7286	5448-5458	JJ	denotes	homologous
T7287	5459-5472	NN	denotes	recombination
T7288	5473-5475	IN	denotes	at
T7289	5476-5479	DT	denotes	the
T7291	5480-5490	JJ	denotes	endogenous
T7292	5491-5496	NN	denotes	Acadm
T7290	5497-5502	NN	denotes	locus
T7293	5503-5512	VBG	denotes	resulting
T7294	5513-5515	IN	denotes	in
T7295	5516-5517	DT	denotes	a
T7296	5518-5529	NN	denotes	duplication
T7297	5530-5532	IN	denotes	of
T7298	5533-5538	NNS	denotes	exons
T7299	5539-5540	CD	denotes	8
T7300	5540-5542	,	denotes	,
T7301	5542-5543	CD	denotes	9
T7302	5543-5545	,	denotes	,
T7303	5545-5548	CC	denotes	and
T7304	5549-5551	CD	denotes	10
T7305	5552-5554	IN	denotes	at
T7306	5555-5558	DT	denotes	the
T7308	5559-5568	VBN	denotes	disrupted
T7307	5569-5575	NN	denotes	allele
T7309	5575-5576	.	denotes	.
T7310	5576-5664	sentence	denotes	(B) Southern blot analysis of EcoRI-digested genomic DNA from ES cells screened by PCR.
T7311	5577-5578	-LRB-	denotes	(
T7312	5578-5579	LS	denotes	B
T7314	5579-5580	-RRB-	denotes	)
T7315	5581-5589	NNP	denotes	Southern
T7316	5590-5594	NN	denotes	blot
T7313	5595-5603	NN	denotes	analysis
T7317	5604-5606	IN	denotes	of
T7318	5607-5612	NN	denotes	EcoRI
T7320	5612-5613	HYPH	denotes	-
T7319	5613-5621	VBN	denotes	digested
T7322	5622-5629	JJ	denotes	genomic
T7321	5630-5633	NN	denotes	DNA
T7323	5634-5638	IN	denotes	from
T7324	5639-5641	NN	denotes	ES
T7325	5642-5647	NNS	denotes	cells
T7326	5648-5656	VBN	denotes	screened
T7327	5657-5659	IN	denotes	by
T7328	5660-5663	NN	denotes	PCR
T7329	5663-5664	.	denotes	.
T7330	5664-5871	sentence	denotes	Probe A, a DNA fragment consisting of a portion of exon 10 that is not present in the targeting vector, hybridizes to an endogenous 3.1-kb fragment and, upon homologous recombination, to a 13.2-kb fragment.
T7331	5665-5670	NN	denotes	Probe
T7332	5671-5672	NN	denotes	A
T7334	5672-5674	,	denotes	,
T7335	5674-5675	DT	denotes	a
T7337	5676-5679	NN	denotes	DNA
T7336	5680-5688	NN	denotes	fragment
T7338	5689-5699	VBG	denotes	consisting
T7339	5700-5702	IN	denotes	of
T7340	5703-5704	DT	denotes	a
T7341	5705-5712	NN	denotes	portion
T7342	5713-5715	IN	denotes	of
T7343	5716-5720	NN	denotes	exon
T7344	5721-5723	CD	denotes	10
T7345	5724-5728	WDT	denotes	that
T7346	5729-5731	VBZ	denotes	is
T7347	5732-5735	RB	denotes	not
T7348	5736-5743	JJ	denotes	present
T7349	5744-5746	IN	denotes	in
T7350	5747-5750	DT	denotes	the
T7352	5751-5760	NN	denotes	targeting
T7351	5761-5767	NN	denotes	vector
T7353	5767-5769	,	denotes	,
T7333	5769-5779	VBZ	denotes	hybridizes
T7354	5780-5782	IN	denotes	to
T7355	5783-5785	DT	denotes	an
T7357	5786-5796	JJ	denotes	endogenous
T7358	5797-5800	CD	denotes	3.1
T7360	5800-5801	HYPH	denotes	-
T7359	5801-5803	NN	denotes	kb
T7356	5804-5812	NN	denotes	fragment
T7361	5813-5816	CC	denotes	and
T7362	5816-5818	,	denotes	,
T7363	5818-5822	IN	denotes	upon
T7365	5823-5833	JJ	denotes	homologous
T7366	5834-5847	NN	denotes	recombination
T7367	5847-5849	,	denotes	,
T7364	5849-5851	IN	denotes	to
T7368	5852-5853	DT	denotes	a
T7370	5854-5858	CD	denotes	13.2
T7372	5858-5859	HYPH	denotes	-
T7371	5859-5861	NN	denotes	kb
T7369	5862-5870	NN	denotes	fragment
T7373	5870-5871	.	denotes	.
T7374	5871-5966	sentence	denotes	Lane 1 represents a wild-type ES cell line, and Lane 2 and 3 represent targeted ES cell lines.
T7375	5872-5876	NN	denotes	Lane
T7377	5877-5878	CD	denotes	1
T7376	5879-5889	VBZ	denotes	represents
T7378	5890-5891	DT	denotes	a
T7380	5892-5896	JJ	denotes	wild
T7382	5896-5897	HYPH	denotes	-
T7381	5897-5901	NN	denotes	type
T7383	5902-5904	NN	denotes	ES
T7384	5905-5909	NN	denotes	cell
T7379	5910-5914	NN	denotes	line
T7385	5914-5916	,	denotes	,
T7386	5916-5919	CC	denotes	and
T7387	5920-5924	NN	denotes	Lane
T7388	5925-5926	CD	denotes	2
T7390	5927-5930	CC	denotes	and
T7391	5931-5932	CD	denotes	3
T7389	5933-5942	VBP	denotes	represent
T7392	5943-5951	VBN	denotes	targeted
T7394	5952-5954	NN	denotes	ES
T7395	5955-5959	NN	denotes	cell
T7393	5960-5965	NNS	denotes	lines
T7396	5965-5966	.	denotes	.
T1138	5967-5969	NN	denotes	ES
T1139	5970-5974	NN	denotes	cell
T1140	5975-5981	NNS	denotes	clones
T1142	5982-5986	VBD	denotes	were
T1141	5987-5995	VBN	denotes	screened
T1143	5996-5998	IN	denotes	by
T1144	5999-6002	NN	denotes	PCR
T1145	6003-6004	-LRB-	denotes	(
T1147	6004-6008	NNS	denotes	data
T1148	6009-6012	RB	denotes	not
T1146	6013-6018	VBN	denotes	shown
T1149	6018-6019	-RRB-	denotes	)
T1150	6020-6023	CC	denotes	and
T1151	6024-6033	VBN	denotes	confirmed
T1152	6034-6036	IN	denotes	by
T1153	6037-6045	NNP	denotes	Southern
T1154	6046-6050	NN	denotes	blot
T1155	6051-6059	NN	denotes	analysis
T1156	6059-6060	.	denotes	.
T1157	6060-6300	sentence	denotes	Southern blot analysis used an exon 10 probe (probe A), not present in the targeting vector, hybridized to a 13.2-kb band in addition to the 3.1-kb endogenous band indicating targeted insertion of the vector at the Acadm locus (Figure 1B).
T1158	6061-6069	NNP	denotes	Southern
T1159	6070-6074	NN	denotes	blot
T1160	6075-6083	NN	denotes	analysis
T1161	6084-6088	VBD	denotes	used
T1162	6089-6091	DT	denotes	an
T1164	6092-6096	NN	denotes	exon
T1165	6097-6099	CD	denotes	10
T1163	6100-6105	NN	denotes	probe
T1166	6106-6107	-LRB-	denotes	(
T1168	6107-6112	NN	denotes	probe
T1167	6113-6114	NN	denotes	A
T1169	6114-6115	-RRB-	denotes	)
T1170	6115-6117	,	denotes	,
T1171	6117-6120	RB	denotes	not
T1172	6121-6128	JJ	denotes	present
T1173	6129-6131	IN	denotes	in
T1174	6132-6135	DT	denotes	the
T1176	6136-6145	NN	denotes	targeting
T1175	6146-6152	NN	denotes	vector
T1177	6152-6154	,	denotes	,
T1178	6154-6164	VBN	denotes	hybridized
T1179	6165-6167	IN	denotes	to
T1180	6168-6169	DT	denotes	a
T1182	6170-6174	CD	denotes	13.2
T1184	6174-6175	HYPH	denotes	-
T1183	6175-6177	NN	denotes	kb
T1181	6178-6182	NN	denotes	band
T1185	6183-6185	IN	denotes	in
T1186	6186-6194	NN	denotes	addition
T1187	6195-6197	IN	denotes	to
T1188	6198-6201	DT	denotes	the
T1190	6202-6205	CD	denotes	3.1
T1192	6205-6206	HYPH	denotes	-
T1191	6206-6208	NN	denotes	kb
T1193	6209-6219	JJ	denotes	endogenous
T1189	6220-6224	NN	denotes	band
T1194	6225-6235	VBG	denotes	indicating
T1195	6236-6244	VBN	denotes	targeted
T1196	6245-6254	NN	denotes	insertion
T1197	6255-6257	IN	denotes	of
T1198	6258-6261	DT	denotes	the
T1199	6262-6268	NN	denotes	vector
T1200	6269-6271	IN	denotes	at
T1201	6272-6275	DT	denotes	the
T1203	6276-6281	NN	denotes	Acadm
T1202	6282-6287	NN	denotes	locus
T1204	6288-6289	-LRB-	denotes	(
T1205	6289-6295	NN	denotes	Figure
T1206	6296-6298	CD	denotes	1B
T1207	6298-6299	-RRB-	denotes	)
T1208	6299-6300	.	denotes	.
T1209	6300-6414	sentence	denotes	Correctly targeted ES cell clones were microinjected into B6 (C57BL/6NTac) blastocysts to generate chimeric mice.
T1210	6301-6310	RB	denotes	Correctly
T1211	6311-6319	VBN	denotes	targeted
T1213	6320-6322	NN	denotes	ES
T1214	6323-6327	NN	denotes	cell
T1212	6328-6334	NNS	denotes	clones
T1216	6335-6339	VBD	denotes	were
T1215	6340-6353	VBN	denotes	microinjected
T1217	6354-6358	IN	denotes	into
T1218	6359-6361	NN	denotes	B6
T1220	6362-6363	-LRB-	denotes	(
T1221	6363-6368	NN	denotes	C57BL
T1223	6368-6369	HYPH	denotes	/
T1222	6369-6374	NN	denotes	6NTac
T1224	6374-6375	-RRB-	denotes	)
T1219	6376-6387	NNS	denotes	blastocysts
T1225	6388-6390	TO	denotes	to
T1226	6391-6399	VB	denotes	generate
T1227	6400-6408	JJ	denotes	chimeric
T1228	6409-6413	NNS	denotes	mice
T1229	6413-6414	.	denotes	.
T1230	6414-6555	sentence	denotes	Chimeric mice were backcrossed to both 129P2 and B6 inbred mice to produce MCAD+/− and eventually MCAD−/− mice on a B6/129 mixed background.
T1231	6415-6423	JJ	denotes	Chimeric
T1232	6424-6428	NNS	denotes	mice
T1234	6429-6433	VBD	denotes	were
T1233	6434-6445	VBN	denotes	backcrossed
T1235	6446-6448	IN	denotes	to
T1236	6449-6453	CC	denotes	both
T1237	6454-6459	NN	denotes	129P2
T1239	6460-6463	CC	denotes	and
T1240	6464-6466	NN	denotes	B6
T1238	6467-6473	VBN	denotes	inbred
T1241	6474-6478	NNS	denotes	mice
T1242	6479-6481	TO	denotes	to
T1243	6482-6489	VB	denotes	produce
T1244	6490-6494	NN	denotes	MCAD
T1246	6494-6495	SYM	denotes	+
T1247	6495-6496	HYPH	denotes	/
T1248	6496-6497	SYM	denotes	−
T1249	6498-6501	CC	denotes	and
T1250	6502-6512	RB	denotes	eventually
T1251	6513-6517	NN	denotes	MCAD
T1252	6517-6518	SYM	denotes	−
T1253	6518-6519	HYPH	denotes	/
T1254	6519-6520	SYM	denotes	−
T1245	6521-6525	NNS	denotes	mice
T1255	6526-6528	IN	denotes	on
T1256	6529-6530	DT	denotes	a
T1258	6531-6533	NN	denotes	B6
T1259	6533-6534	HYPH	denotes	/
T1260	6534-6537	CD	denotes	129
T1261	6538-6543	JJ	denotes	mixed
T1257	6544-6554	NN	denotes	background
T1262	6554-6555	.	denotes	.
T1263	6555-6747	sentence	denotes	The studies described here were conducted exclusively on the B6/129 mixed background compared with littermate controls or B6/129 control groups maintained by intercrosses as were the mutants.
T1264	6556-6559	DT	denotes	The
T1265	6560-6567	NNS	denotes	studies
T1267	6568-6577	VBN	denotes	described
T1268	6578-6582	RB	denotes	here
T1269	6583-6587	VBD	denotes	were
T1266	6588-6597	VBN	denotes	conducted
T1270	6598-6609	RB	denotes	exclusively
T1271	6610-6612	IN	denotes	on
T1272	6613-6616	DT	denotes	the
T1274	6617-6619	NN	denotes	B6
T1275	6619-6620	HYPH	denotes	/
T1276	6620-6623	CD	denotes	129
T1277	6624-6629	JJ	denotes	mixed
T1273	6630-6640	NN	denotes	background
T1278	6641-6649	VBN	denotes	compared
T1279	6650-6654	IN	denotes	with
T1280	6655-6665	NN	denotes	littermate
T1281	6666-6674	NNS	denotes	controls
T1282	6675-6677	CC	denotes	or
T1283	6678-6680	NN	denotes	B6
T1285	6680-6681	HYPH	denotes	/
T1286	6681-6684	CD	denotes	129
T1287	6685-6692	NN	denotes	control
T1284	6693-6699	NNS	denotes	groups
T1288	6700-6710	VBN	denotes	maintained
T1289	6711-6713	IN	denotes	by
T1290	6714-6726	NNS	denotes	intercrosses
T1291	6727-6729	IN	denotes	as
T1292	6730-6734	VBD	denotes	were
T1293	6735-6738	DT	denotes	the
T1294	6739-6746	NNS	denotes	mutants
T1295	6746-6747	.	denotes	.
T1296	6747-6844	sentence	denotes	Perpetuating this mutation as a congenic mutant line on the 129P2 background proved impractical.
T1297	6748-6760	VBG	denotes	Perpetuating
T1299	6761-6765	DT	denotes	this
T1300	6766-6774	NN	denotes	mutation
T1301	6775-6777	IN	denotes	as
T1302	6778-6779	DT	denotes	a
T1304	6780-6788	JJ	denotes	congenic
T1305	6789-6795	NN	denotes	mutant
T1303	6796-6800	NN	denotes	line
T1306	6801-6803	IN	denotes	on
T1307	6804-6807	DT	denotes	the
T1309	6808-6813	NN	denotes	129P2
T1308	6814-6824	NN	denotes	background
T1298	6825-6831	VBD	denotes	proved
T1310	6832-6843	JJ	denotes	impractical
T1311	6843-6844	.	denotes	.
T1312	6844-7012	sentence	denotes	The 129P2 mice were poor breeders as wild-types, and when introduced, the Acadm mutation was nearly lost on this background because of the high rate of neonatal death.
T1313	6845-6848	DT	denotes	The
T1315	6849-6854	NN	denotes	129P2
T1314	6855-6859	NNS	denotes	mice
T1316	6860-6864	VBD	denotes	were
T1317	6865-6869	JJ	denotes	poor
T1318	6870-6878	NNS	denotes	breeders
T1319	6879-6881	IN	denotes	as
T1320	6882-6886	JJ	denotes	wild
T1322	6886-6887	HYPH	denotes	-
T1321	6887-6892	NNS	denotes	types
T1323	6892-6894	,	denotes	,
T1324	6894-6897	CC	denotes	and
T1325	6898-6902	WRB	denotes	when
T1326	6903-6913	VBN	denotes	introduced
T1328	6913-6915	,	denotes	,
T1329	6915-6918	DT	denotes	the
T1331	6919-6924	NN	denotes	Acadm
T1330	6925-6933	NN	denotes	mutation
T1332	6934-6937	VBD	denotes	was
T1333	6938-6944	RB	denotes	nearly
T1327	6945-6949	VBN	denotes	lost
T1334	6950-6952	IN	denotes	on
T1335	6953-6957	DT	denotes	this
T1336	6958-6968	NN	denotes	background
T1337	6969-6976	IN	denotes	because
T1338	6977-6979	IN	denotes	of
T1339	6980-6983	DT	denotes	the
T1341	6984-6988	JJ	denotes	high
T1340	6989-6993	NN	denotes	rate
T1342	6994-6996	IN	denotes	of
T1343	6997-7005	JJ	denotes	neonatal
T1344	7006-7011	NN	denotes	death
T1345	7011-7012	.	denotes	.
T1346	7012-7148	sentence	denotes	Because of the molecular structure of the targeted allele, it proved virtually impossible to distinguish all three potential genotypes.
T1347	7013-7020	IN	denotes	Because
T1349	7021-7023	IN	denotes	of
T1350	7024-7027	DT	denotes	the
T1352	7028-7037	JJ	denotes	molecular
T1351	7038-7047	NN	denotes	structure
T1353	7048-7050	IN	denotes	of
T1354	7051-7054	DT	denotes	the
T1356	7055-7063	VBN	denotes	targeted
T1355	7064-7070	NN	denotes	allele
T1357	7070-7072	,	denotes	,
T1358	7072-7074	PRP	denotes	it
T1348	7075-7081	VBD	denotes	proved
T1359	7082-7091	RB	denotes	virtually
T1360	7092-7102	JJ	denotes	impossible
T1361	7103-7105	TO	denotes	to
T1362	7106-7117	VB	denotes	distinguish
T1363	7118-7121	DT	denotes	all
T1365	7122-7127	CD	denotes	three
T1366	7128-7137	JJ	denotes	potential
T1364	7138-7147	NNS	denotes	genotypes
T1367	7147-7148	.	denotes	.
T1368	7148-7343	sentence	denotes	We could clearly detect the presence or absence of the targeted allele, however, whether a particular mouse was MCAD−/− or MCAD+/− could not be determined by Southern blot or PCR of genomic DNA.
T1369	7149-7151	PRP	denotes	We
T1371	7152-7157	MD	denotes	could
T1372	7158-7165	RB	denotes	clearly
T1370	7166-7172	VB	denotes	detect
T1374	7173-7176	DT	denotes	the
T1375	7177-7185	NN	denotes	presence
T1376	7186-7188	CC	denotes	or
T1377	7189-7196	NN	denotes	absence
T1378	7197-7199	IN	denotes	of
T1379	7200-7203	DT	denotes	the
T1381	7204-7212	VBN	denotes	targeted
T1380	7213-7219	NN	denotes	allele
T1382	7219-7221	,	denotes	,
T1383	7221-7228	RB	denotes	however
T1384	7228-7230	,	denotes	,
T1385	7230-7237	IN	denotes	whether
T1387	7238-7239	DT	denotes	a
T1389	7240-7250	JJ	denotes	particular
T1388	7251-7256	NN	denotes	mouse
T1386	7257-7260	VBD	denotes	was
T1390	7261-7265	NN	denotes	MCAD
T1391	7265-7266	SYM	denotes	−
T1392	7266-7267	HYPH	denotes	/
T1393	7267-7268	SYM	denotes	−
T1394	7269-7271	CC	denotes	or
T1395	7272-7276	NN	denotes	MCAD
T1396	7276-7277	SYM	denotes	+
T1397	7277-7278	HYPH	denotes	/
T1398	7278-7279	SYM	denotes	−
T1399	7280-7285	MD	denotes	could
T1400	7286-7289	RB	denotes	not
T1401	7290-7292	VB	denotes	be
T1373	7293-7303	VBN	denotes	determined
T1402	7304-7306	IN	denotes	by
T1403	7307-7315	NNP	denotes	Southern
T1404	7316-7320	NN	denotes	blot
T1405	7321-7323	CC	denotes	or
T1406	7324-7327	NN	denotes	PCR
T1407	7328-7330	IN	denotes	of
T1408	7331-7338	JJ	denotes	genomic
T1409	7339-7342	NN	denotes	DNA
T1410	7342-7343	.	denotes	.
T1411	7343-7497	sentence	denotes	Ultimately MCAD−/− mice were ascertained by immunoblot analysis of offspring with subsequent perpetuation of MCAD−/− and MCAD+/+ mice as separate groups.
T1412	7344-7354	RB	denotes	Ultimately
T1414	7355-7359	NN	denotes	MCAD
T1416	7359-7360	SYM	denotes	−
T1417	7360-7361	HYPH	denotes	/
T1418	7361-7362	SYM	denotes	−
T1415	7363-7367	NNS	denotes	mice
T1419	7368-7372	VBD	denotes	were
T1413	7373-7384	VBN	denotes	ascertained
T1420	7385-7387	IN	denotes	by
T1421	7388-7398	NN	denotes	immunoblot
T1422	7399-7407	NN	denotes	analysis
T1423	7408-7410	IN	denotes	of
T1424	7411-7420	NN	denotes	offspring
T1425	7421-7425	IN	denotes	with
T1426	7426-7436	JJ	denotes	subsequent
T1427	7437-7449	NN	denotes	perpetuation
T1428	7450-7452	IN	denotes	of
T1429	7453-7457	NN	denotes	MCAD
T1431	7457-7458	SYM	denotes	−
T1432	7458-7459	HYPH	denotes	/
T1433	7459-7460	SYM	denotes	−
T1434	7461-7464	CC	denotes	and
T1435	7465-7469	NN	denotes	MCAD
T1436	7469-7470	SYM	denotes	+
T1437	7470-7471	HYPH	denotes	/
T1438	7471-7472	SYM	denotes	+
T1430	7473-7477	NNS	denotes	mice
T1439	7478-7480	IN	denotes	as
T1440	7481-7489	JJ	denotes	separate
T1441	7490-7496	NNS	denotes	groups
T1442	7496-7497	.	denotes	.
T1606	7499-7502	NN	denotes	RNA
T1607	7503-7511	NN	denotes	Analysis
T1608	7511-7707	sentence	denotes	RT-PCR amplification from exon 7 to 11 from total heart RNA amplified the expected 600-base pair (bp) fragment in MCAD+/+ and MCAD+/− mice, and a 1.5-kb fragment in MCAD−/− mice (data not shown).
T1609	7512-7514	NN	denotes	RT
T1611	7514-7515	HYPH	denotes	-
T1610	7515-7518	NN	denotes	PCR
T1612	7519-7532	NN	denotes	amplification
T1614	7533-7537	IN	denotes	from
T1615	7538-7542	NN	denotes	exon
T1616	7543-7544	CD	denotes	7
T1617	7545-7547	IN	denotes	to
T1618	7548-7550	CD	denotes	11
T1619	7551-7555	IN	denotes	from
T1620	7556-7561	JJ	denotes	total
T1622	7562-7567	NN	denotes	heart
T1621	7568-7571	NN	denotes	RNA
T1613	7572-7581	VBD	denotes	amplified
T1623	7582-7585	DT	denotes	the
T1625	7586-7594	VBN	denotes	expected
T1626	7595-7598	CD	denotes	600
T1628	7598-7599	HYPH	denotes	-
T1629	7599-7603	NN	denotes	base
T1627	7604-7608	NN	denotes	pair
T1630	7609-7610	-LRB-	denotes	(
T1631	7610-7612	NN	denotes	bp
T1632	7612-7613	-RRB-	denotes	)
T1624	7614-7622	NN	denotes	fragment
T1633	7623-7625	IN	denotes	in
T1634	7626-7630	NN	denotes	MCAD
T1636	7630-7631	SYM	denotes	+
T1637	7631-7632	HYPH	denotes	/
T1638	7632-7633	SYM	denotes	+
T1639	7634-7637	CC	denotes	and
T1640	7638-7642	NN	denotes	MCAD
T1641	7642-7643	SYM	denotes	+
T1642	7643-7644	HYPH	denotes	/
T1643	7644-7645	SYM	denotes	−
T1635	7646-7650	NNS	denotes	mice
T1644	7650-7652	,	denotes	,
T1645	7652-7655	CC	denotes	and
T1646	7656-7657	DT	denotes	a
T1648	7658-7661	CD	denotes	1.5
T1650	7661-7662	HYPH	denotes	-
T1649	7662-7664	NN	denotes	kb
T1647	7665-7673	NN	denotes	fragment
T1651	7674-7676	IN	denotes	in
T1652	7677-7681	NN	denotes	MCAD
T1654	7681-7682	SYM	denotes	−
T1655	7682-7683	HYPH	denotes	/
T1656	7683-7684	SYM	denotes	−
T1653	7685-7689	NNS	denotes	mice
T1657	7690-7691	-LRB-	denotes	(
T1659	7691-7695	NNS	denotes	data
T1660	7696-7699	RB	denotes	not
T1658	7700-7705	VBN	denotes	shown
T1661	7705-7706	-RRB-	denotes	)
T1662	7706-7707	.	denotes	.
T1663	7707-7877	sentence	denotes	Sequence analysis of the 1.5-kb fragment revealed that the amplified fragment consisted of exon 7 to exon 10 with 280 bp of pGEM plasmid sequence followed by exons 8–11.
T1664	7708-7716	NN	denotes	Sequence
T1665	7717-7725	NN	denotes	analysis
T1667	7726-7728	IN	denotes	of
T1668	7729-7732	DT	denotes	the
T1670	7733-7736	CD	denotes	1.5
T1672	7736-7737	HYPH	denotes	-
T1671	7737-7739	NN	denotes	kb
T1669	7740-7748	NN	denotes	fragment
T1666	7749-7757	VBD	denotes	revealed
T1673	7758-7762	IN	denotes	that
T1675	7763-7766	DT	denotes	the
T1677	7767-7776	VBN	denotes	amplified
T1676	7777-7785	NN	denotes	fragment
T1674	7786-7795	VBD	denotes	consisted
T1678	7796-7798	IN	denotes	of
T1679	7799-7803	NN	denotes	exon
T1680	7804-7805	CD	denotes	7
T1681	7806-7808	IN	denotes	to
T1682	7809-7813	NN	denotes	exon
T1683	7814-7816	CD	denotes	10
T1684	7817-7821	IN	denotes	with
T1685	7822-7825	CD	denotes	280
T1686	7826-7828	NN	denotes	bp
T1687	7829-7831	IN	denotes	of
T1688	7832-7836	NN	denotes	pGEM
T1690	7837-7844	NN	denotes	plasmid
T1689	7845-7853	NN	denotes	sequence
T1691	7854-7862	VBN	denotes	followed
T1692	7863-7865	IN	denotes	by
T1693	7866-7871	NNS	denotes	exons
T1694	7872-7873	CD	denotes	8
T1695	7873-7874	SYM	denotes	–
T1696	7874-7876	CD	denotes	11
T1697	7876-7877	.	denotes	.
T1698	7877-7980	sentence	denotes	Some of the plasmid sequences, along with the pPGKNEOpA sequence, were deleted from this spliced mRNA.
T1699	7878-7882	DT	denotes	Some
T1701	7883-7885	IN	denotes	of
T1702	7886-7889	DT	denotes	the
T1704	7890-7897	NN	denotes	plasmid
T1703	7898-7907	NNS	denotes	sequences
T1705	7907-7909	,	denotes	,
T1706	7909-7914	IN	denotes	along
T1707	7915-7919	IN	denotes	with
T1708	7920-7923	DT	denotes	the
T1710	7924-7933	NN	denotes	pPGKNEOpA
T1709	7934-7942	NN	denotes	sequence
T1711	7942-7944	,	denotes	,
T1712	7944-7948	VBD	denotes	were
T1700	7949-7956	VBN	denotes	deleted
T1713	7957-7961	IN	denotes	from
T1714	7962-7966	DT	denotes	this
T1716	7967-7974	VBN	denotes	spliced
T1715	7975-7979	NN	denotes	mRNA
T1717	7979-7980	.	denotes	.
T1718	7980-8253	sentence	denotes	Northern blot analysis revealed Acadm was normally expressed in all tissues analyzed from MCAD+/+ mice with the most robust expression occurring in brown fat, kidney, heart, skeletal muscle, and liver with minimal expression in the brain, white fat, and testes (Figure 2).
T1719	7981-7989	NNP	denotes	Northern
T1720	7990-7994	NN	denotes	blot
T1721	7995-8003	NN	denotes	analysis
T1722	8004-8012	VBD	denotes	revealed
T1723	8013-8018	NN	denotes	Acadm
T1725	8019-8022	VBD	denotes	was
T1726	8023-8031	RB	denotes	normally
T1724	8032-8041	VBN	denotes	expressed
T1727	8042-8044	IN	denotes	in
T1728	8045-8048	DT	denotes	all
T1729	8049-8056	NNS	denotes	tissues
T1730	8057-8065	VBN	denotes	analyzed
T1731	8066-8070	IN	denotes	from
T1732	8071-8075	NN	denotes	MCAD
T1734	8075-8076	SYM	denotes	+
T1735	8076-8077	HYPH	denotes	/
T1736	8077-8078	SYM	denotes	+
T1733	8079-8083	NNS	denotes	mice
T1737	8084-8088	IN	denotes	with
T1738	8089-8092	DT	denotes	the
T1740	8093-8097	RBS	denotes	most
T1741	8098-8104	JJ	denotes	robust
T1739	8105-8115	NN	denotes	expression
T1742	8116-8125	VBG	denotes	occurring
T1743	8126-8128	IN	denotes	in
T1744	8129-8134	JJ	denotes	brown
T1745	8135-8138	NN	denotes	fat
T1746	8138-8140	,	denotes	,
T1747	8140-8146	NN	denotes	kidney
T1748	8146-8148	,	denotes	,
T1749	8148-8153	NN	denotes	heart
T1750	8153-8155	,	denotes	,
T1751	8155-8163	JJ	denotes	skeletal
T1752	8164-8170	NN	denotes	muscle
T1753	8170-8172	,	denotes	,
T1754	8172-8175	CC	denotes	and
T1755	8176-8181	NN	denotes	liver
T1756	8182-8186	IN	denotes	with
T1757	8187-8194	JJ	denotes	minimal
T1758	8195-8205	NN	denotes	expression
T1759	8206-8208	IN	denotes	in
T1760	8209-8212	DT	denotes	the
T1761	8213-8218	NN	denotes	brain
T1762	8218-8220	,	denotes	,
T1763	8220-8225	JJ	denotes	white
T1764	8226-8229	NN	denotes	fat
T1765	8229-8231	,	denotes	,
T1766	8231-8234	CC	denotes	and
T1767	8235-8241	NNS	denotes	testes
T1768	8242-8243	-LRB-	denotes	(
T1769	8243-8249	NN	denotes	Figure
T1770	8250-8251	CD	denotes	2
T1771	8251-8252	-RRB-	denotes	)
T1772	8252-8253	.	denotes	.
T1773	8253-8415	sentence	denotes	Interestingly, although RT-PCR amplified an incorrectly spliced Acadm transcript, no Acadm transcripts were detected by northern blot analysis from MCAD−/− mice.
T1774	8254-8267	RB	denotes	Interestingly
T1776	8267-8269	,	denotes	,
T1777	8269-8277	IN	denotes	although
T1779	8278-8280	NN	denotes	RT
T1781	8280-8281	HYPH	denotes	-
T1780	8281-8284	NN	denotes	PCR
T1778	8285-8294	VBD	denotes	amplified
T1782	8295-8297	DT	denotes	an
T1784	8298-8309	RB	denotes	incorrectly
T1785	8310-8317	VBN	denotes	spliced
T1786	8318-8323	NN	denotes	Acadm
T1783	8324-8334	NN	denotes	transcript
T1787	8334-8336	,	denotes	,
T1788	8336-8338	DT	denotes	no
T1790	8339-8344	NN	denotes	Acadm
T1789	8345-8356	NNS	denotes	transcripts
T1791	8357-8361	VBD	denotes	were
T1775	8362-8370	VBN	denotes	detected
T1792	8371-8373	IN	denotes	by
T1793	8374-8382	NNP	denotes	northern
T1794	8383-8387	NN	denotes	blot
T1795	8388-8396	NN	denotes	analysis
T1796	8397-8401	IN	denotes	from
T1797	8402-8406	NN	denotes	MCAD
T1799	8406-8407	SYM	denotes	−
T1800	8407-8408	HYPH	denotes	/
T1801	8408-8409	SYM	denotes	−
T1798	8410-8414	NNS	denotes	mice
T1802	8414-8415	.	denotes	.
T1803	8415-8557	sentence	denotes	These results strongly suggest that the mutant RNA is unstable and degraded rapidly or, alternatively, undergoes nonsense mediated RNA decay.
T1804	8416-8421	DT	denotes	These
T1805	8422-8429	NNS	denotes	results
T1807	8430-8438	RB	denotes	strongly
T1806	8439-8446	VBP	denotes	suggest
T1808	8447-8451	IN	denotes	that
T1810	8452-8455	DT	denotes	the
T1812	8456-8462	NN	denotes	mutant
T1811	8463-8466	NN	denotes	RNA
T1809	8467-8469	VBZ	denotes	is
T1813	8470-8478	JJ	denotes	unstable
T1814	8479-8482	CC	denotes	and
T1815	8483-8491	VBN	denotes	degraded
T1816	8492-8499	RB	denotes	rapidly
T1817	8500-8502	CC	denotes	or
T1818	8502-8504	,	denotes	,
T1819	8504-8517	RB	denotes	alternatively
T1821	8517-8519	,	denotes	,
T1820	8519-8528	VBZ	denotes	undergoes
T1822	8529-8537	NN	denotes	nonsense
T1823	8538-8546	VBN	denotes	mediated
T1825	8547-8550	NN	denotes	RNA
T1824	8551-8556	NN	denotes	decay
T1826	8556-8557	.	denotes	.
T7455	8568-8576	NNP	denotes	Northern
T7456	8577-8581	NN	denotes	Blot
T7457	8582-8590	NN	denotes	Analysis
T7458	8591-8595	IN	denotes	from
T7459	8596-8600	NN	denotes	MCAD
T7461	8600-8601	SYM	denotes	−
T7462	8601-8602	HYPH	denotes	/
T7463	8602-8603	SYM	denotes	−
T7464	8604-8605	-LRB-	denotes	(
T7466	8605-8606	NN	denotes	n
T7467	8607-8608	SYM	denotes	=
T7465	8609-8610	CD	denotes	2
T7468	8610-8611	-RRB-	denotes	)
T7469	8612-8615	CC	denotes	and
T7470	8616-8620	NN	denotes	MCAD
T7471	8620-8621	SYM	denotes	+
T7472	8621-8622	HYPH	denotes	/
T7473	8622-8623	SYM	denotes	+
T7474	8624-8625	-LRB-	denotes	(
T7476	8625-8626	NN	denotes	n
T7477	8627-8628	SYM	denotes	=
T7475	8629-8630	CD	denotes	2
T7478	8630-8631	-RRB-	denotes	)
T7460	8632-8636	NNS	denotes	Mice
T7479	8636-8788	sentence	denotes	Acadm message was detected from the heart, liver, brown fat, brain, kidney, and muscle (and white fat and testes, data not shown) of only MCAD+/+ mice.
T7480	8637-8642	NN	denotes	Acadm
T7481	8643-8650	NN	denotes	message
T7483	8651-8654	VBD	denotes	was
T7482	8655-8663	VBN	denotes	detected
T7484	8664-8668	IN	denotes	from
T7485	8669-8672	DT	denotes	the
T7486	8673-8678	NN	denotes	heart
T7487	8678-8680	,	denotes	,
T7488	8680-8685	NN	denotes	liver
T7489	8685-8687	,	denotes	,
T7490	8687-8692	JJ	denotes	brown
T7491	8693-8696	NN	denotes	fat
T7492	8696-8698	,	denotes	,
T7493	8698-8703	NN	denotes	brain
T7494	8703-8705	,	denotes	,
T7495	8705-8711	NN	denotes	kidney
T7496	8711-8713	,	denotes	,
T7497	8713-8716	CC	denotes	and
T7498	8717-8723	NN	denotes	muscle
T7499	8724-8725	-LRB-	denotes	(
T7500	8725-8728	CC	denotes	and
T7501	8729-8734	JJ	denotes	white
T7502	8735-8738	NN	denotes	fat
T7503	8739-8742	CC	denotes	and
T7504	8743-8749	NNS	denotes	testes
T7505	8749-8751	,	denotes	,
T7507	8751-8755	NNS	denotes	data
T7508	8756-8759	RB	denotes	not
T7506	8760-8765	VBN	denotes	shown
T7509	8765-8766	-RRB-	denotes	)
T7510	8767-8769	IN	denotes	of
T7511	8770-8774	JJ	denotes	only
T7513	8775-8779	NN	denotes	MCAD
T7514	8779-8780	SYM	denotes	+
T7515	8780-8781	HYPH	denotes	/
T7516	8781-8782	SYM	denotes	+
T7512	8783-8787	NNS	denotes	mice
T7517	8787-8788	.	denotes	.
T7518	8788-8870	sentence	denotes	Most robust expression occurred in brown fat, kidney, heart, and skeletal muscle.
T7519	8789-8793	RBS	denotes	Most
T7521	8794-8800	JJ	denotes	robust
T7520	8801-8811	NN	denotes	expression
T7522	8812-8820	VBD	denotes	occurred
T7523	8821-8823	IN	denotes	in
T7524	8824-8829	JJ	denotes	brown
T7525	8830-8833	NN	denotes	fat
T7526	8833-8835	,	denotes	,
T7527	8835-8841	NN	denotes	kidney
T7528	8841-8843	,	denotes	,
T7529	8843-8848	NN	denotes	heart
T7530	8848-8850	,	denotes	,
T7531	8850-8853	CC	denotes	and
T7532	8854-8862	JJ	denotes	skeletal
T7533	8863-8869	NN	denotes	muscle
T7534	8869-8870	.	denotes	.
T7535	8870-8934	sentence	denotes	MCAD−/− mice had no detectable message in all tissues examined.
T7536	8871-8875	NN	denotes	MCAD
T7538	8875-8876	SYM	denotes	−
T7539	8876-8877	HYPH	denotes	/
T7540	8877-8878	SYM	denotes	−
T7537	8879-8883	NNS	denotes	mice
T7541	8884-8887	VBD	denotes	had
T7542	8888-8890	DT	denotes	no
T7544	8891-8901	JJ	denotes	detectable
T7543	8902-8909	NN	denotes	message
T7545	8910-8912	IN	denotes	in
T7546	8913-8916	DT	denotes	all
T7547	8917-8924	NNS	denotes	tissues
T7548	8925-8933	VBN	denotes	examined
T7549	8933-8934	.	denotes	.
T1979	8936-8941	NN	denotes	Liver
T1980	8942-8948	NN	denotes	Enzyme
T1981	8949-8957	NNS	denotes	Analyses
T1982	8957-9129	sentence	denotes	Immunoblot analyses of liver homogenates with anti-MCAD antisera demonstrated that the 42 kDa MCAD monomer was present in MCAD+/+ mice, but not in MCAD−/− mice (Figure 3).
T1983	8958-8968	NN	denotes	Immunoblot
T1984	8969-8977	NNS	denotes	analyses
T1986	8978-8980	IN	denotes	of
T1987	8981-8986	NN	denotes	liver
T1988	8987-8998	NNS	denotes	homogenates
T1989	8999-9003	IN	denotes	with
T1990	9004-9013	JJ	denotes	anti-MCAD
T1991	9014-9022	NNS	denotes	antisera
T1985	9023-9035	VBD	denotes	demonstrated
T1992	9036-9040	IN	denotes	that
T1994	9041-9044	DT	denotes	the
T1996	9045-9047	CD	denotes	42
T1997	9048-9051	NN	denotes	kDa
T1998	9052-9056	NN	denotes	MCAD
T1995	9057-9064	NN	denotes	monomer
T1993	9065-9068	VBD	denotes	was
T1999	9069-9076	JJ	denotes	present
T2000	9077-9079	IN	denotes	in
T2001	9080-9084	NN	denotes	MCAD
T2003	9084-9085	SYM	denotes	+
T2004	9085-9086	HYPH	denotes	/
T2005	9086-9087	SYM	denotes	+
T2002	9088-9092	NNS	denotes	mice
T2006	9092-9094	,	denotes	,
T2007	9094-9097	CC	denotes	but
T2008	9098-9101	RB	denotes	not
T2009	9102-9104	IN	denotes	in
T2010	9105-9109	NN	denotes	MCAD
T2012	9109-9110	SYM	denotes	−
T2013	9110-9111	HYPH	denotes	/
T2014	9111-9112	SYM	denotes	−
T2011	9113-9117	NNS	denotes	mice
T2015	9118-9119	-LRB-	denotes	(
T2016	9119-9125	NN	denotes	Figure
T2017	9126-9127	CD	denotes	3
T2018	9127-9128	-RRB-	denotes	)
T2019	9128-9129	.	denotes	.
T2020	9129-9317	sentence	denotes	As a control analysis, anti–short-chain acyl-CoA dehydrogenase (SCAD) antisera revealed no differences in levels of expression of SCAD protein between MCAD+/+ and MCAD−/− mice (Figure 3).
T2021	9130-9132	IN	denotes	As
T2023	9133-9134	DT	denotes	a
T2025	9135-9142	NN	denotes	control
T2024	9143-9151	NN	denotes	analysis
T2026	9151-9153	,	denotes	,
T2027	9153-9163	JJ	denotes	anti–short
T2029	9163-9164	HYPH	denotes	-
T2028	9164-9169	NN	denotes	chain
T2031	9170-9174	NN	denotes	acyl
T2033	9174-9175	HYPH	denotes	-
T2032	9175-9178	NN	denotes	CoA
T2030	9179-9192	NN	denotes	dehydrogenase
T2035	9193-9194	-LRB-	denotes	(
T2034	9194-9198	NN	denotes	SCAD
T2037	9198-9199	-RRB-	denotes	)
T2036	9200-9208	NNS	denotes	antisera
T2022	9209-9217	VBD	denotes	revealed
T2038	9218-9220	DT	denotes	no
T2039	9221-9232	NNS	denotes	differences
T2040	9233-9235	IN	denotes	in
T2041	9236-9242	NNS	denotes	levels
T2042	9243-9245	IN	denotes	of
T2043	9246-9256	NN	denotes	expression
T2044	9257-9259	IN	denotes	of
T2045	9260-9264	NN	denotes	SCAD
T2046	9265-9272	NN	denotes	protein
T2047	9273-9280	IN	denotes	between
T2048	9281-9285	NN	denotes	MCAD
T2050	9285-9286	SYM	denotes	+
T2051	9286-9287	HYPH	denotes	/
T2052	9287-9288	SYM	denotes	+
T2053	9289-9292	CC	denotes	and
T2054	9293-9297	NN	denotes	MCAD
T2055	9297-9298	SYM	denotes	−
T2056	9298-9299	HYPH	denotes	/
T2057	9299-9300	SYM	denotes	−
T2049	9301-9305	NNS	denotes	mice
T2058	9306-9307	-LRB-	denotes	(
T2059	9307-9313	NN	denotes	Figure
T2060	9314-9315	CD	denotes	3
T2061	9315-9316	-RRB-	denotes	)
T2062	9316-9317	.	denotes	.
T2063	9317-10043	sentence	denotes	Figure 3 Immunoblots of Liver Homogenates from MCAD+/+ and MCAD−/− Mice These were probed with anti-MCAD antibody or anti-SCAD antibody. Homozygous MCAD−/− mice had no detectable MCAD protein. MCAD protein is only detectable under the MCAD protein–spiked (positive control) lane. As a control analysis, liver homogenates probed with anti-SCAD antibody revealed that SCAD protein was present in both MCAD+/+ and MCAD−/− mice. No MCAD positive-control protein is detected by anti-SCAD antibodies (MCAD lane). mw, molecular weight standards. Enzyme activity was analyzed in mouse liver homogenates using the electron transport flavoprotein (ETF) reduction assay with octanoyl-CoA (C8:0) and palmitoyl-CoA (C16:0) as substrates.
T7638	9328-9339	NNS	denotes	Immunoblots
T7639	9340-9342	IN	denotes	of
T7640	9343-9348	NN	denotes	Liver
T7641	9349-9360	NNS	denotes	Homogenates
T7642	9361-9365	IN	denotes	from
T7643	9366-9370	NN	denotes	MCAD
T7645	9370-9371	SYM	denotes	+
T7646	9371-9372	HYPH	denotes	/
T7647	9372-9373	SYM	denotes	+
T7648	9374-9377	CC	denotes	and
T7649	9378-9382	NN	denotes	MCAD
T7650	9382-9383	SYM	denotes	−
T7651	9383-9384	HYPH	denotes	/
T7652	9384-9385	SYM	denotes	−
T7644	9386-9390	NNS	denotes	Mice
T7653	9390-9455	sentence	denotes	These were probed with anti-MCAD antibody or anti-SCAD antibody.
T7654	9391-9396	DT	denotes	These
T7656	9397-9401	VBD	denotes	were
T7655	9402-9408	VBN	denotes	probed
T7657	9409-9413	IN	denotes	with
T7658	9414-9423	JJ	denotes	anti-MCAD
T7659	9424-9432	NN	denotes	antibody
T7660	9433-9435	CC	denotes	or
T7661	9436-9445	JJ	denotes	anti-SCAD
T7662	9446-9454	NN	denotes	antibody
T7663	9454-9455	.	denotes	.
T7664	9455-9511	sentence	denotes	Homozygous MCAD−/− mice had no detectable MCAD protein.
T7665	9456-9466	JJ	denotes	Homozygous
T7667	9467-9471	NN	denotes	MCAD
T7668	9471-9472	SYM	denotes	−
T7669	9472-9473	HYPH	denotes	/
T7670	9473-9474	SYM	denotes	−
T7666	9475-9479	NNS	denotes	mice
T7671	9480-9483	VBD	denotes	had
T7672	9484-9486	DT	denotes	no
T7674	9487-9497	JJ	denotes	detectable
T7675	9498-9502	NN	denotes	MCAD
T7673	9503-9510	NN	denotes	protein
T7676	9510-9511	.	denotes	.
T7677	9511-9598	sentence	denotes	MCAD protein is only detectable under the MCAD protein–spiked (positive control) lane.
T7678	9512-9516	NN	denotes	MCAD
T7679	9517-9524	NN	denotes	protein
T7680	9525-9527	VBZ	denotes	is
T7681	9528-9532	RB	denotes	only
T7682	9533-9543	JJ	denotes	detectable
T7683	9544-9549	IN	denotes	under
T7684	9550-9553	DT	denotes	the
T7686	9554-9558	NN	denotes	MCAD
T7687	9559-9566	NN	denotes	protein
T7689	9566-9567	HYPH	denotes	–
T7688	9567-9573	VBN	denotes	spiked
T7690	9574-9575	-LRB-	denotes	(
T7692	9575-9583	JJ	denotes	positive
T7691	9584-9591	NN	denotes	control
T7693	9591-9592	-RRB-	denotes	)
T7685	9593-9597	NN	denotes	lane
T7694	9597-9598	.	denotes	.
T7695	9598-9743	sentence	denotes	As a control analysis, liver homogenates probed with anti-SCAD antibody revealed that SCAD protein was present in both MCAD+/+ and MCAD−/− mice.
T7696	9599-9601	IN	denotes	As
T7698	9602-9603	DT	denotes	a
T7700	9604-9611	NN	denotes	control
T7699	9612-9620	NN	denotes	analysis
T7701	9620-9622	,	denotes	,
T7702	9622-9627	NN	denotes	liver
T7703	9628-9639	NNS	denotes	homogenates
T7704	9640-9646	VBN	denotes	probed
T7705	9647-9651	IN	denotes	with
T7706	9652-9661	JJ	denotes	anti-SCAD
T7707	9662-9670	NN	denotes	antibody
T7697	9671-9679	VBD	denotes	revealed
T7708	9680-9684	IN	denotes	that
T7710	9685-9689	NN	denotes	SCAD
T7711	9690-9697	NN	denotes	protein
T7709	9698-9701	VBD	denotes	was
T7712	9702-9709	JJ	denotes	present
T7713	9710-9712	IN	denotes	in
T7714	9713-9717	CC	denotes	both
T7715	9718-9722	NN	denotes	MCAD
T7717	9722-9723	SYM	denotes	+
T7718	9723-9724	HYPH	denotes	/
T7719	9724-9725	SYM	denotes	+
T7720	9726-9729	CC	denotes	and
T7721	9730-9734	NN	denotes	MCAD
T7722	9734-9735	SYM	denotes	−
T7723	9735-9736	HYPH	denotes	/
T7724	9736-9737	SYM	denotes	−
T7716	9738-9742	NNS	denotes	mice
T7725	9742-9743	.	denotes	.
T7726	9743-9825	sentence	denotes	No MCAD positive-control protein is detected by anti-SCAD antibodies (MCAD lane).
T7727	9744-9746	DT	denotes	No
T7729	9747-9751	NN	denotes	MCAD
T7730	9752-9760	JJ	denotes	positive
T7732	9760-9761	HYPH	denotes	-
T7731	9761-9768	NN	denotes	control
T7728	9769-9776	NN	denotes	protein
T7734	9777-9779	VBZ	denotes	is
T7733	9780-9788	VBN	denotes	detected
T7735	9789-9791	IN	denotes	by
T7736	9792-9801	JJ	denotes	anti-SCAD
T7737	9802-9812	NNS	denotes	antibodies
T7738	9813-9814	-LRB-	denotes	(
T7740	9814-9818	NN	denotes	MCAD
T7739	9819-9823	NN	denotes	lane
T7741	9823-9824	-RRB-	denotes	)
T7742	9824-9825	.	denotes	.
T7743	9825-9857	sentence	denotes	mw, molecular weight standards.
T7744	9826-9828	NN	denotes	mw
T7745	9828-9830	,	denotes	,
T7746	9830-9839	JJ	denotes	molecular
T7748	9840-9846	NN	denotes	weight
T7747	9847-9856	NNS	denotes	standards
T7749	9856-9857	.	denotes	.
T2064	9858-9864	NN	denotes	Enzyme
T2065	9865-9873	NN	denotes	activity
T2067	9874-9877	VBD	denotes	was
T2066	9878-9886	VBN	denotes	analyzed
T2068	9887-9889	IN	denotes	in
T2069	9890-9895	NN	denotes	mouse
T2071	9896-9901	NN	denotes	liver
T2070	9902-9913	NNS	denotes	homogenates
T2072	9914-9919	VBG	denotes	using
T2073	9920-9923	DT	denotes	the
T2075	9924-9932	NN	denotes	electron
T2077	9933-9942	NN	denotes	transport
T2076	9943-9955	NN	denotes	flavoprotein
T2078	9956-9957	-LRB-	denotes	(
T2079	9957-9960	NN	denotes	ETF
T2080	9960-9961	-RRB-	denotes	)
T2081	9962-9971	NN	denotes	reduction
T2074	9972-9977	NN	denotes	assay
T2082	9978-9982	IN	denotes	with
T2083	9983-9991	NN	denotes	octanoyl
T2085	9991-9992	HYPH	denotes	-
T2084	9992-9995	NN	denotes	CoA
T2086	9996-9997	-LRB-	denotes	(
T2087	9997-9999	NN	denotes	C8
T2088	9999-10000	:	denotes	:
T2089	10000-10001	CD	denotes	0
T2090	10001-10002	-RRB-	denotes	)
T2091	10003-10006	CC	denotes	and
T2092	10007-10016	NN	denotes	palmitoyl
T2094	10016-10017	HYPH	denotes	-
T2093	10017-10020	NN	denotes	CoA
T2095	10021-10022	-LRB-	denotes	(
T2096	10022-10025	NN	denotes	C16
T2097	10025-10026	:	denotes	:
T2098	10026-10027	CD	denotes	0
T2099	10027-10028	-RRB-	denotes	)
T2100	10029-10031	IN	denotes	as
T2101	10032-10042	NNS	denotes	substrates
T2102	10042-10043	.	denotes	.
T2103	10043-10217	sentence	denotes	MCAD−/− mice had a significant reduction in ability to dehydrogenate octanoyl-CoA and a modest reduction in activity toward palmitoyl-CoA compared to MCAD+/+ mice (Table 1).
T2104	10044-10048	NN	denotes	MCAD
T2106	10048-10049	SYM	denotes	−
T2107	10049-10050	HYPH	denotes	/
T2108	10050-10051	SYM	denotes	−
T2105	10052-10056	NNS	denotes	mice
T2109	10057-10060	VBD	denotes	had
T2110	10061-10062	DT	denotes	a
T2112	10063-10074	JJ	denotes	significant
T2111	10075-10084	NN	denotes	reduction
T2113	10085-10087	IN	denotes	in
T2114	10088-10095	NN	denotes	ability
T2115	10096-10098	TO	denotes	to
T2116	10099-10112	VB	denotes	dehydrogenate
T2117	10113-10121	NN	denotes	octanoyl
T2119	10121-10122	HYPH	denotes	-
T2118	10122-10125	NN	denotes	CoA
T2120	10126-10129	CC	denotes	and
T2121	10130-10131	DT	denotes	a
T2123	10132-10138	JJ	denotes	modest
T2122	10139-10148	NN	denotes	reduction
T2124	10149-10151	IN	denotes	in
T2125	10152-10160	NN	denotes	activity
T2126	10161-10167	IN	denotes	toward
T2127	10168-10177	NN	denotes	palmitoyl
T2129	10177-10178	HYPH	denotes	-
T2128	10178-10181	NN	denotes	CoA
T2130	10182-10190	VBN	denotes	compared
T2131	10191-10193	IN	denotes	to
T2132	10194-10198	NN	denotes	MCAD
T2134	10198-10199	SYM	denotes	+
T2135	10199-10200	HYPH	denotes	/
T2136	10200-10201	SYM	denotes	+
T2133	10202-10206	NNS	denotes	mice
T2137	10207-10208	-LRB-	denotes	(
T2138	10208-10213	NN	denotes	Table
T2139	10214-10215	CD	denotes	1
T2140	10215-10216	-RRB-	denotes	)
T2141	10216-10217	.	denotes	.
T2142	10217-10391	sentence	denotes	Specifically, the dehydrogenation of octanoyl-CoA and palmitoyl-CoA substrates were reduced by 75% and by 30%, respectively, in MCAD−/− mice as compared to MCAD+/+ controls.
T2143	10218-10230	RB	denotes	Specifically
T2145	10230-10232	,	denotes	,
T2146	10232-10235	DT	denotes	the
T2147	10236-10251	NN	denotes	dehydrogenation
T2148	10252-10254	IN	denotes	of
T2149	10255-10263	NN	denotes	octanoyl
T2151	10263-10264	HYPH	denotes	-
T2150	10264-10267	NN	denotes	CoA
T2153	10268-10271	CC	denotes	and
T2154	10272-10281	NN	denotes	palmitoyl
T2156	10281-10282	HYPH	denotes	-
T2155	10282-10285	NN	denotes	CoA
T2152	10286-10296	NNS	denotes	substrates
T2157	10297-10301	VBD	denotes	were
T2144	10302-10309	VBN	denotes	reduced
T2158	10310-10312	IN	denotes	by
T2159	10313-10315	CD	denotes	75
T2160	10315-10316	NN	denotes	%
T2161	10317-10320	CC	denotes	and
T2162	10321-10323	IN	denotes	by
T2163	10324-10326	CD	denotes	30
T2164	10326-10327	NN	denotes	%
T2165	10327-10329	,	denotes	,
T2166	10329-10341	RB	denotes	respectively
T2167	10341-10343	,	denotes	,
T2168	10343-10345	IN	denotes	in
T2169	10346-10350	NN	denotes	MCAD
T2171	10350-10351	SYM	denotes	−
T2172	10351-10352	HYPH	denotes	/
T2173	10352-10353	SYM	denotes	−
T2170	10354-10358	NNS	denotes	mice
T2174	10359-10361	IN	denotes	as
T2175	10362-10370	VBN	denotes	compared
T2176	10371-10373	IN	denotes	to
T2177	10374-10378	NN	denotes	MCAD
T2179	10378-10379	SYM	denotes	+
T2180	10379-10380	HYPH	denotes	/
T2181	10380-10381	SYM	denotes	+
T2178	10382-10390	NNS	denotes	controls
T2182	10390-10391	.	denotes	.
T8161	10401-10416	NNS	denotes	Characteristics
T8162	10417-10419	IN	denotes	of
T8163	10420-10424	NN	denotes	MCAD
T8165	10424-10425	HYPH	denotes	-
T8164	10425-10434	JJ	denotes	Deficient
T8166	10435-10439	NNS	denotes	Mice
T2294	10679-10687	JJ	denotes	Neonatal
T2295	10688-10694	NNS	denotes	Deaths
T2296	10694-10752	sentence	denotes	Significant neonatal mortality was noted in MCAD−/− pups.
T2297	10695-10706	JJ	denotes	Significant
T2299	10707-10715	JJ	denotes	neonatal
T2298	10716-10725	NN	denotes	mortality
T2301	10726-10729	VBD	denotes	was
T2300	10730-10735	VBN	denotes	noted
T2302	10736-10738	IN	denotes	in
T2303	10739-10743	NN	denotes	MCAD
T2305	10743-10744	SYM	denotes	−
T2306	10744-10745	HYPH	denotes	/
T2307	10745-10746	SYM	denotes	−
T2304	10747-10751	NNS	denotes	pups
T2308	10751-10752	.	denotes	.
T2309	10752-10915	sentence	denotes	Although equal numbers of pups were born from MCAD+/+ and MCAD−/− mice, only 41% of MCAD−/− mice survived to weaning as compared to 98% of MCAD+/+ mice (Table 1).
T2310	10753-10761	IN	denotes	Although
T2312	10762-10767	JJ	denotes	equal
T2313	10768-10775	NNS	denotes	numbers
T2314	10776-10778	IN	denotes	of
T2315	10779-10783	NNS	denotes	pups
T2316	10784-10788	VBD	denotes	were
T2311	10789-10793	VBN	denotes	born
T2318	10794-10798	IN	denotes	from
T2319	10799-10803	NN	denotes	MCAD
T2321	10803-10804	SYM	denotes	+
T2322	10804-10805	HYPH	denotes	/
T2323	10805-10806	SYM	denotes	+
T2324	10807-10810	CC	denotes	and
T2325	10811-10815	NN	denotes	MCAD
T2326	10815-10816	SYM	denotes	−
T2327	10816-10817	HYPH	denotes	/
T2328	10817-10818	SYM	denotes	−
T2320	10819-10823	NNS	denotes	mice
T2329	10823-10825	,	denotes	,
T2330	10825-10829	RB	denotes	only
T2332	10830-10832	CD	denotes	41
T2331	10832-10833	NN	denotes	%
T2333	10834-10836	IN	denotes	of
T2334	10837-10841	NN	denotes	MCAD
T2336	10841-10842	SYM	denotes	−
T2337	10842-10843	HYPH	denotes	/
T2338	10843-10844	SYM	denotes	−
T2335	10845-10849	NNS	denotes	mice
T2317	10850-10858	VBD	denotes	survived
T2339	10859-10861	IN	denotes	to
T2340	10862-10869	NN	denotes	weaning
T2341	10870-10872	IN	denotes	as
T2342	10873-10881	VBN	denotes	compared
T2343	10882-10884	IN	denotes	to
T2344	10885-10887	CD	denotes	98
T2345	10887-10888	NN	denotes	%
T2346	10889-10891	IN	denotes	of
T2347	10892-10896	NN	denotes	MCAD
T2349	10896-10897	SYM	denotes	+
T2350	10897-10898	HYPH	denotes	/
T2351	10898-10899	SYM	denotes	+
T2348	10900-10904	NNS	denotes	mice
T2352	10905-10906	-LRB-	denotes	(
T2353	10906-10911	NN	denotes	Table
T2354	10912-10913	CD	denotes	1
T2355	10913-10914	-RRB-	denotes	)
T2356	10914-10915	.	denotes	.
T2357	10915-10968	sentence	denotes	The mechanism of neonatal loss remains undetermined.
T2358	10916-10919	DT	denotes	The
T2359	10920-10929	NN	denotes	mechanism
T2361	10930-10932	IN	denotes	of
T2362	10933-10941	JJ	denotes	neonatal
T2363	10942-10946	NN	denotes	loss
T2360	10947-10954	VBZ	denotes	remains
T2364	10955-10967	JJ	denotes	undetermined
T2365	10967-10968	.	denotes	.
T2366	10968-11054	sentence	denotes	The MCAD−/− pups are abandoned more frequently than MCAD+/+ pups for unknown reasons.
T2367	10969-10972	DT	denotes	The
T2369	10973-10977	NN	denotes	MCAD
T2370	10977-10978	SYM	denotes	−
T2371	10978-10979	HYPH	denotes	/
T2372	10979-10980	SYM	denotes	−
T2368	10981-10985	NNS	denotes	pups
T2374	10986-10989	VBP	denotes	are
T2373	10990-10999	VBN	denotes	abandoned
T2375	11000-11004	RBR	denotes	more
T2376	11005-11015	RB	denotes	frequently
T2377	11016-11020	IN	denotes	than
T2378	11021-11025	NN	denotes	MCAD
T2380	11025-11026	SYM	denotes	+
T2381	11026-11027	HYPH	denotes	/
T2382	11027-11028	SYM	denotes	+
T2379	11029-11033	NNS	denotes	pups
T2383	11034-11037	IN	denotes	for
T2384	11038-11045	JJ	denotes	unknown
T2385	11046-11053	NNS	denotes	reasons
T2386	11053-11054	.	denotes	.
T2387	11054-11110	sentence	denotes	They are likely more prone to hypothermia than MCAD+/+.
T2388	11055-11059	PRP	denotes	They
T2389	11060-11063	VBP	denotes	are
T2390	11064-11070	RB	denotes	likely
T2391	11071-11075	RBR	denotes	more
T2392	11076-11081	JJ	denotes	prone
T2393	11082-11084	IN	denotes	to
T2394	11085-11096	NN	denotes	hypothermia
T2395	11097-11101	IN	denotes	than
T2396	11102-11106	NN	denotes	MCAD
T2397	11106-11107	SYM	denotes	+
T2398	11107-11108	HYPH	denotes	/
T2399	11108-11109	SYM	denotes	+
T2400	11109-11110	.	denotes	.
T2401	11110-11342	sentence	denotes	Because of the difficulty of distinguishing the MCAD−/− mutants from the MCAD+/− heterozygous pups by molecular analysis due to the insertion mutation, we could only compare MCAD+/+ × MCAD+/+ matings with MCAD−/− × MCAD−/− matings.
T2402	11111-11118	IN	denotes	Because
T2404	11119-11121	IN	denotes	of
T2405	11122-11125	DT	denotes	the
T2406	11126-11136	NN	denotes	difficulty
T2407	11137-11139	IN	denotes	of
T2408	11140-11154	VBG	denotes	distinguishing
T2409	11155-11158	DT	denotes	the
T2411	11159-11163	NN	denotes	MCAD
T2412	11163-11164	SYM	denotes	−
T2413	11164-11165	HYPH	denotes	/
T2414	11165-11166	SYM	denotes	−
T2410	11167-11174	NNS	denotes	mutants
T2415	11175-11179	IN	denotes	from
T2416	11180-11183	DT	denotes	the
T2418	11184-11188	NN	denotes	MCAD
T2419	11188-11189	SYM	denotes	+
T2420	11189-11190	HYPH	denotes	/
T2421	11190-11191	SYM	denotes	−
T2422	11192-11204	JJ	denotes	heterozygous
T2417	11205-11209	NNS	denotes	pups
T2423	11210-11212	IN	denotes	by
T2424	11213-11222	JJ	denotes	molecular
T2425	11223-11231	NN	denotes	analysis
T2426	11232-11235	IN	denotes	due
T2427	11236-11238	IN	denotes	to
T2428	11239-11242	DT	denotes	the
T2430	11243-11252	NN	denotes	insertion
T2429	11253-11261	NN	denotes	mutation
T2431	11261-11263	,	denotes	,
T2432	11263-11265	PRP	denotes	we
T2433	11266-11271	MD	denotes	could
T2434	11272-11276	RB	denotes	only
T2403	11277-11284	VB	denotes	compare
T2435	11285-11289	NN	denotes	MCAD
T2437	11289-11290	SYM	denotes	+
T2438	11290-11291	HYPH	denotes	/
T2439	11291-11292	SYM	denotes	+
T2440	11293-11294	SYM	denotes	×
T2441	11295-11299	NN	denotes	MCAD
T2442	11299-11300	SYM	denotes	+
T2443	11300-11301	HYPH	denotes	/
T2444	11301-11302	SYM	denotes	+
T2436	11303-11310	NNS	denotes	matings
T2445	11311-11315	IN	denotes	with
T2446	11316-11320	NN	denotes	MCAD
T2448	11320-11321	SYM	denotes	−
T2449	11321-11322	HYPH	denotes	/
T2450	11322-11323	SYM	denotes	−
T2451	11324-11325	SYM	denotes	×
T2452	11326-11330	NN	denotes	MCAD
T2453	11330-11331	SYM	denotes	−
T2454	11331-11332	HYPH	denotes	/
T2455	11332-11333	SYM	denotes	−
T2447	11334-11341	NNS	denotes	matings
T2456	11341-11342	.	denotes	.
T2457	11342-11416	sentence	denotes	Thus, we were unable to evaluate the pedigrees from heterozygous matings.
T2458	11343-11347	RB	denotes	Thus
T2460	11347-11349	,	denotes	,
T2461	11349-11351	PRP	denotes	we
T2459	11352-11356	VBD	denotes	were
T2462	11357-11363	JJ	denotes	unable
T2463	11364-11366	TO	denotes	to
T2464	11367-11375	VB	denotes	evaluate
T2465	11376-11379	DT	denotes	the
T2466	11380-11389	NNS	denotes	pedigrees
T2467	11390-11394	IN	denotes	from
T2468	11395-11407	JJ	denotes	heterozygous
T2469	11408-11415	NNS	denotes	matings
T2470	11415-11416	.	denotes	.
T2565	11418-11425	NN	denotes	Fasting
T2566	11426-11429	CC	denotes	and
T2567	11430-11434	NN	denotes	Cold
T2568	11435-11446	NN	denotes	Intolerance
T2569	11446-11565	sentence	denotes	In order to examine the stress effects of fasting on MCAD-deficient mice, they were fasted for 24 h prior to analysis.
T2570	11447-11449	IN	denotes	In
T2572	11450-11455	NN	denotes	order
T2573	11456-11458	TO	denotes	to
T2574	11459-11466	VB	denotes	examine
T2575	11467-11470	DT	denotes	the
T2577	11471-11477	NN	denotes	stress
T2576	11478-11485	NNS	denotes	effects
T2578	11486-11488	IN	denotes	of
T2579	11489-11496	NN	denotes	fasting
T2580	11497-11499	IN	denotes	on
T2581	11500-11504	NN	denotes	MCAD
T2583	11504-11505	HYPH	denotes	-
T2582	11505-11514	JJ	denotes	deficient
T2584	11515-11519	NNS	denotes	mice
T2585	11519-11521	,	denotes	,
T2586	11521-11525	PRP	denotes	they
T2587	11526-11530	VBD	denotes	were
T2571	11531-11537	VBN	denotes	fasted
T2588	11538-11541	IN	denotes	for
T2589	11542-11544	CD	denotes	24
T2590	11545-11546	NN	denotes	h
T2591	11547-11552	JJ	denotes	prior
T2592	11553-11555	IN	denotes	to
T2593	11556-11564	NN	denotes	analysis
T2594	11564-11565	.	denotes	.
T2595	11565-11730	sentence	denotes	MCAD−/− mice displayed lower serum glucose and elevated serum free fatty acid levels although neither result was significant, as compared to MCAD+/+ mice (Table 1).
T2596	11566-11570	NN	denotes	MCAD
T2598	11570-11571	SYM	denotes	−
T2599	11571-11572	HYPH	denotes	/
T2600	11572-11573	SYM	denotes	−
T2597	11574-11578	NNS	denotes	mice
T2601	11579-11588	VBD	denotes	displayed
T2602	11589-11594	JJR	denotes	lower
T2604	11595-11600	NN	denotes	serum
T2603	11601-11608	NN	denotes	glucose
T2605	11609-11612	CC	denotes	and
T2606	11613-11621	VBN	denotes	elevated
T2608	11622-11627	NN	denotes	serum
T2609	11628-11632	JJ	denotes	free
T2611	11633-11638	JJ	denotes	fatty
T2610	11639-11643	NN	denotes	acid
T2607	11644-11650	NNS	denotes	levels
T2612	11651-11659	IN	denotes	although
T2614	11660-11667	DT	denotes	neither
T2615	11668-11674	NN	denotes	result
T2613	11675-11678	VBD	denotes	was
T2616	11679-11690	JJ	denotes	significant
T2617	11690-11692	,	denotes	,
T2618	11692-11694	IN	denotes	as
T2619	11695-11703	VBN	denotes	compared
T2620	11704-11706	IN	denotes	to
T2621	11707-11711	NN	denotes	MCAD
T2623	11711-11712	SYM	denotes	+
T2624	11712-11713	HYPH	denotes	/
T2625	11713-11714	SYM	denotes	+
T2622	11715-11719	NNS	denotes	mice
T2626	11720-11721	-LRB-	denotes	(
T2627	11721-11726	NN	denotes	Table
T2628	11727-11728	CD	denotes	1
T2629	11728-11729	-RRB-	denotes	)
T2630	11729-11730	.	denotes	.
T2631	11730-11846	sentence	denotes	To determine the effects of cold stress, mice were fasted for 18 h and placed in 4 °C environment for a 3-h period.
T2632	11731-11733	TO	denotes	To
T2633	11734-11743	VB	denotes	determine
T2635	11744-11747	DT	denotes	the
T2636	11748-11755	NNS	denotes	effects
T2637	11756-11758	IN	denotes	of
T2638	11759-11763	NN	denotes	cold
T2639	11764-11770	NN	denotes	stress
T2640	11770-11772	,	denotes	,
T2641	11772-11776	NNS	denotes	mice
T2642	11777-11781	VBD	denotes	were
T2634	11782-11788	VBN	denotes	fasted
T2643	11789-11792	IN	denotes	for
T2644	11793-11795	CD	denotes	18
T2645	11796-11797	NN	denotes	h
T2646	11798-11801	CC	denotes	and
T2647	11802-11808	VBN	denotes	placed
T2648	11809-11811	IN	denotes	in
T2649	11812-11813	CD	denotes	4
T2650	11814-11816	NN	denotes	°C
T2651	11817-11828	NN	denotes	environment
T2652	11829-11832	IN	denotes	for
T2653	11833-11834	DT	denotes	a
T2655	11835-11836	CD	denotes	3
T2657	11836-11837	HYPH	denotes	-
T2656	11837-11838	NN	denotes	h
T2654	11839-11845	NN	denotes	period
T2658	11845-11846	.	denotes	.
T2659	11846-11979	sentence	denotes	The MCAD−/− mice were significantly compromised within this short period of cold stress, some severe enough to result in fatalities.
T2660	11847-11850	DT	denotes	The
T2662	11851-11855	NN	denotes	MCAD
T2663	11855-11856	SYM	denotes	−
T2664	11856-11857	HYPH	denotes	/
T2665	11857-11858	SYM	denotes	−
T2661	11859-11863	NNS	denotes	mice
T2667	11864-11868	VBD	denotes	were
T2668	11869-11882	RB	denotes	significantly
T2666	11883-11894	VBN	denotes	compromised
T2669	11895-11901	IN	denotes	within
T2670	11902-11906	DT	denotes	this
T2672	11907-11912	JJ	denotes	short
T2671	11913-11919	NN	denotes	period
T2673	11920-11922	IN	denotes	of
T2674	11923-11927	NN	denotes	cold
T2675	11928-11934	NN	denotes	stress
T2676	11934-11936	,	denotes	,
T2677	11936-11940	DT	denotes	some
T2678	11941-11947	JJ	denotes	severe
T2679	11948-11954	RB	denotes	enough
T2680	11955-11957	TO	denotes	to
T2681	11958-11964	VB	denotes	result
T2682	11965-11967	IN	denotes	in
T2683	11968-11978	NNS	denotes	fatalities
T2684	11978-11979	.	denotes	.
T2685	11979-12128	sentence	denotes	After 1 h of the cold challenge, the average rectal temperature of MCAD−/− mice (n = 5) was 23.4 °C as compared with 35 °C for MCAD+/+ mice (n = 4).
T2686	11980-11985	IN	denotes	After
T2688	11986-11987	CD	denotes	1
T2689	11988-11989	NN	denotes	h
T2690	11990-11992	IN	denotes	of
T2691	11993-11996	DT	denotes	the
T2693	11997-12001	NN	denotes	cold
T2692	12002-12011	NN	denotes	challenge
T2694	12011-12013	,	denotes	,
T2695	12013-12016	DT	denotes	the
T2697	12017-12024	JJ	denotes	average
T2698	12025-12031	JJ	denotes	rectal
T2696	12032-12043	NN	denotes	temperature
T2699	12044-12046	IN	denotes	of
T2700	12047-12051	NN	denotes	MCAD
T2702	12051-12052	SYM	denotes	−
T2703	12052-12053	HYPH	denotes	/
T2704	12053-12054	SYM	denotes	−
T2701	12055-12059	NNS	denotes	mice
T2705	12060-12061	-LRB-	denotes	(
T2707	12061-12062	NN	denotes	n
T2708	12063-12064	SYM	denotes	=
T2706	12065-12066	CD	denotes	5
T2709	12066-12067	-RRB-	denotes	)
T2687	12068-12071	VBD	denotes	was
T2710	12072-12076	CD	denotes	23.4
T2711	12077-12079	NN	denotes	°C
T2712	12080-12082	IN	denotes	as
T2713	12083-12091	VBN	denotes	compared
T2714	12092-12096	IN	denotes	with
T2715	12097-12099	CD	denotes	35
T2716	12100-12102	NN	denotes	°C
T2717	12103-12106	IN	denotes	for
T2718	12107-12111	NN	denotes	MCAD
T2720	12111-12112	SYM	denotes	+
T2721	12112-12113	HYPH	denotes	/
T2722	12113-12114	SYM	denotes	+
T2719	12115-12119	NNS	denotes	mice
T2723	12120-12121	-LRB-	denotes	(
T2725	12121-12122	NN	denotes	n
T2726	12123-12124	SYM	denotes	=
T2724	12125-12126	CD	denotes	4
T2727	12126-12127	-RRB-	denotes	)
T2728	12127-12128	.	denotes	.
T2729	12128-12238	sentence	denotes	Rectal temperatures declined to unrecoverable temperatures of 16.7–19.2 °C in three of the five MCAD−/− mice.
T2730	12129-12135	JJ	denotes	Rectal
T2731	12136-12148	NNS	denotes	temperatures
T2732	12149-12157	VBD	denotes	declined
T2733	12158-12160	IN	denotes	to
T2734	12161-12174	JJ	denotes	unrecoverable
T2735	12175-12187	NNS	denotes	temperatures
T2736	12188-12190	IN	denotes	of
T2737	12191-12195	CD	denotes	16.7
T2739	12195-12196	SYM	denotes	–
T2738	12196-12200	CD	denotes	19.2
T2740	12201-12203	NN	denotes	°C
T2741	12204-12206	IN	denotes	in
T2742	12207-12212	CD	denotes	three
T2744	12213-12215	IN	denotes	of
T2745	12216-12219	DT	denotes	the
T2743	12220-12224	CD	denotes	five
T2747	12225-12229	NN	denotes	MCAD
T2748	12229-12230	SYM	denotes	−
T2749	12230-12231	HYPH	denotes	/
T2750	12231-12232	SYM	denotes	−
T2746	12233-12237	NNS	denotes	mice
T2751	12237-12238	.	denotes	.
T2752	12238-12317	sentence	denotes	By the end of the 1.5-h mark, the two surviving MCAD−/− mice averaged 22.7 °C.
T2753	12239-12241	IN	denotes	By
T2755	12242-12245	DT	denotes	the
T2756	12246-12249	NN	denotes	end
T2757	12250-12252	IN	denotes	of
T2758	12253-12256	DT	denotes	the
T2760	12257-12260	CD	denotes	1.5
T2762	12260-12261	HYPH	denotes	-
T2761	12261-12262	NN	denotes	h
T2759	12263-12267	NN	denotes	mark
T2763	12267-12269	,	denotes	,
T2764	12269-12272	DT	denotes	the
T2766	12273-12276	CD	denotes	two
T2767	12277-12286	VBG	denotes	surviving
T2768	12287-12291	NN	denotes	MCAD
T2769	12291-12292	SYM	denotes	−
T2770	12292-12293	HYPH	denotes	/
T2771	12293-12294	SYM	denotes	−
T2765	12295-12299	NNS	denotes	mice
T2754	12300-12308	VBD	denotes	averaged
T2772	12309-12313	CD	denotes	22.7
T2773	12314-12316	NN	denotes	°C
T2774	12316-12317	.	denotes	.
T2775	12317-12436	sentence	denotes	In contrast, all four MCAD+/+ mice survived the 3-h cold stress, ending with an average rectal temperature of 33.6 °C.
T2776	12318-12320	IN	denotes	In
T2778	12321-12329	NN	denotes	contrast
T2779	12329-12331	,	denotes	,
T2780	12331-12334	DT	denotes	all
T2782	12335-12339	CD	denotes	four
T2783	12340-12344	NN	denotes	MCAD
T2784	12344-12345	SYM	denotes	+
T2785	12345-12346	HYPH	denotes	/
T2786	12346-12347	SYM	denotes	+
T2781	12348-12352	NNS	denotes	mice
T2777	12353-12361	VBD	denotes	survived
T2787	12362-12365	DT	denotes	the
T2789	12366-12367	CD	denotes	3
T2791	12367-12368	HYPH	denotes	-
T2790	12368-12369	NN	denotes	h
T2792	12370-12374	NN	denotes	cold
T2788	12375-12381	NN	denotes	stress
T2793	12381-12383	,	denotes	,
T2794	12383-12389	VBG	denotes	ending
T2795	12390-12394	IN	denotes	with
T2796	12395-12397	DT	denotes	an
T2798	12398-12405	JJ	denotes	average
T2799	12406-12412	JJ	denotes	rectal
T2797	12413-12424	NN	denotes	temperature
T2800	12425-12427	IN	denotes	of
T2801	12428-12432	CD	denotes	33.6
T2802	12433-12435	NN	denotes	°C
T2803	12435-12436	.	denotes	.
T2908	12438-12445	JJ	denotes	Organic
T2909	12446-12450	NN	denotes	Acid
T2910	12451-12454	CC	denotes	and
T2911	12455-12468	NN	denotes	Acylcarnitine
T2912	12469-12477	NN	denotes	Analysis
T2913	12477-12608	sentence	denotes	Urine organic acid analysis revealed that MCAD−/− mice developed an organic acid profile similar to MCAD-deficient human patients.
T2914	12478-12483	NN	denotes	Urine
T2916	12484-12491	JJ	denotes	organic
T2915	12492-12496	NN	denotes	acid
T2917	12497-12505	NN	denotes	analysis
T2918	12506-12514	VBD	denotes	revealed
T2919	12515-12519	IN	denotes	that
T2921	12520-12524	NN	denotes	MCAD
T2923	12524-12525	SYM	denotes	−
T2924	12525-12526	HYPH	denotes	/
T2925	12526-12527	SYM	denotes	−
T2922	12528-12532	NNS	denotes	mice
T2920	12533-12542	VBD	denotes	developed
T2926	12543-12545	DT	denotes	an
T2928	12546-12553	JJ	denotes	organic
T2929	12554-12558	NN	denotes	acid
T2927	12559-12566	NN	denotes	profile
T2930	12567-12574	JJ	denotes	similar
T2931	12575-12577	IN	denotes	to
T2932	12578-12582	NN	denotes	MCAD
T2934	12582-12583	HYPH	denotes	-
T2933	12583-12592	JJ	denotes	deficient
T2936	12593-12598	JJ	denotes	human
T2935	12599-12607	NNS	denotes	patients
T2937	12607-12608	.	denotes	.
T2938	12608-12877	sentence	denotes	Specifically, when fasted for 18 h, MCAD−/− mice developed significantly elevated concentrations of adipic, suberic, and sebacic acids and hexanoylglycine as compared to MCAD+/+ controls, which showed trace to no detectable amounts of the same organic acids (Table 1).
T2939	12609-12621	RB	denotes	Specifically
T2941	12621-12623	,	denotes	,
T2942	12623-12627	WRB	denotes	when
T2943	12628-12634	VBN	denotes	fasted
T2944	12635-12638	IN	denotes	for
T2945	12639-12641	CD	denotes	18
T2946	12642-12643	NN	denotes	h
T2947	12643-12645	,	denotes	,
T2948	12645-12649	NN	denotes	MCAD
T2950	12649-12650	SYM	denotes	−
T2951	12650-12651	HYPH	denotes	/
T2952	12651-12652	SYM	denotes	−
T2949	12653-12657	NNS	denotes	mice
T2940	12658-12667	VBD	denotes	developed
T2953	12668-12681	RB	denotes	significantly
T2954	12682-12690	VBN	denotes	elevated
T2955	12691-12705	NNS	denotes	concentrations
T2956	12706-12708	IN	denotes	of
T2957	12709-12715	JJ	denotes	adipic
T2959	12715-12717	,	denotes	,
T2960	12717-12724	JJ	denotes	suberic
T2961	12724-12726	,	denotes	,
T2962	12726-12729	CC	denotes	and
T2963	12730-12737	JJ	denotes	sebacic
T2958	12738-12743	NNS	denotes	acids
T2964	12744-12747	CC	denotes	and
T2965	12748-12763	NN	denotes	hexanoylglycine
T2966	12764-12766	IN	denotes	as
T2967	12767-12775	VBN	denotes	compared
T2968	12776-12778	IN	denotes	to
T2969	12779-12783	NN	denotes	MCAD
T2971	12783-12784	SYM	denotes	+
T2972	12784-12785	HYPH	denotes	/
T2973	12785-12786	SYM	denotes	+
T2970	12787-12795	NNS	denotes	controls
T2974	12795-12797	,	denotes	,
T2975	12797-12802	WDT	denotes	which
T2976	12803-12809	VBD	denotes	showed
T2977	12810-12815	NN	denotes	trace
T2978	12816-12818	IN	denotes	to
T2979	12819-12821	DT	denotes	no
T2981	12822-12832	JJ	denotes	detectable
T2980	12833-12840	NNS	denotes	amounts
T2982	12841-12843	IN	denotes	of
T2983	12844-12847	DT	denotes	the
T2985	12848-12852	JJ	denotes	same
T2986	12853-12860	JJ	denotes	organic
T2984	12861-12866	NNS	denotes	acids
T2987	12867-12868	-LRB-	denotes	(
T2988	12868-12873	NN	denotes	Table
T2989	12874-12875	CD	denotes	1
T2990	12875-12876	-RRB-	denotes	)
T2991	12876-12877	.	denotes	.
T2992	12877-12925	sentence	denotes	Adipic acid is not specific to MCAD deficiency.
T2993	12878-12884	JJ	denotes	Adipic
T2994	12885-12889	NN	denotes	acid
T2995	12890-12892	VBZ	denotes	is
T2996	12893-12896	RB	denotes	not
T2997	12897-12905	JJ	denotes	specific
T2998	12906-12908	IN	denotes	to
T2999	12909-12913	NN	denotes	MCAD
T3000	12914-12924	NN	denotes	deficiency
T3001	12924-12925	.	denotes	.
T3002	12925-13069	sentence	denotes	We also evaluated β-hydroxybutyric and acetoacetic concentrations and found no significant differences between MCAD genotypes (data not shown).
T3003	12926-12928	PRP	denotes	We
T3005	12929-12933	RB	denotes	also
T3004	12934-12943	VBD	denotes	evaluated
T3006	12944-12945	NN	denotes	β
T3008	12945-12946	HYPH	denotes	-
T3007	12946-12960	JJ	denotes	hydroxybutyric
T3010	12961-12964	CC	denotes	and
T3011	12965-12976	JJ	denotes	acetoacetic
T3009	12977-12991	NNS	denotes	concentrations
T3012	12992-12995	CC	denotes	and
T3013	12996-13001	VBD	denotes	found
T3014	13002-13004	DT	denotes	no
T3016	13005-13016	JJ	denotes	significant
T3015	13017-13028	NNS	denotes	differences
T3017	13029-13036	IN	denotes	between
T3018	13037-13041	NN	denotes	MCAD
T3019	13042-13051	NNS	denotes	genotypes
T3020	13052-13053	-LRB-	denotes	(
T3022	13053-13057	NNS	denotes	data
T3023	13058-13061	RB	denotes	not
T3021	13062-13067	VBN	denotes	shown
T3024	13067-13068	-RRB-	denotes	)
T3025	13068-13069	.	denotes	.
T3026	13069-13341	sentence	denotes	Comparison of MCAD+/+ and MCAD−/− mice revealed no significant differences in total serum carnitine concentrations between MCAD+/+ and MCAD−/− mice, but MCAD−/− mice had a 5- to 6-fold elevation of serum decenoylcarnitine evident in the acylcarnitine profile (Figure 4A).
T3027	13070-13080	NN	denotes	Comparison
T3029	13081-13083	IN	denotes	of
T3030	13084-13088	NN	denotes	MCAD
T3032	13088-13089	SYM	denotes	+
T3033	13089-13090	HYPH	denotes	/
T3034	13090-13091	SYM	denotes	+
T3035	13092-13095	CC	denotes	and
T3036	13096-13100	NN	denotes	MCAD
T3037	13100-13101	SYM	denotes	−
T3038	13101-13102	HYPH	denotes	/
T3039	13102-13103	SYM	denotes	−
T3031	13104-13108	NNS	denotes	mice
T3028	13109-13117	VBD	denotes	revealed
T3040	13118-13120	DT	denotes	no
T3042	13121-13132	JJ	denotes	significant
T3041	13133-13144	NNS	denotes	differences
T3043	13145-13147	IN	denotes	in
T3044	13148-13153	JJ	denotes	total
T3046	13154-13159	NN	denotes	serum
T3047	13160-13169	NN	denotes	carnitine
T3045	13170-13184	NNS	denotes	concentrations
T3048	13185-13192	IN	denotes	between
T3049	13193-13197	NN	denotes	MCAD
T3051	13197-13198	SYM	denotes	+
T3052	13198-13199	HYPH	denotes	/
T3053	13199-13200	SYM	denotes	+
T3054	13201-13204	CC	denotes	and
T3055	13205-13209	NN	denotes	MCAD
T3056	13209-13210	SYM	denotes	−
T3057	13210-13211	HYPH	denotes	/
T3058	13211-13212	SYM	denotes	−
T3050	13213-13217	NNS	denotes	mice
T3059	13217-13219	,	denotes	,
T3060	13219-13222	CC	denotes	but
T3061	13223-13227	NN	denotes	MCAD
T3063	13227-13228	SYM	denotes	−
T3064	13228-13229	HYPH	denotes	/
T3065	13229-13230	SYM	denotes	−
T3062	13231-13235	NNS	denotes	mice
T3066	13236-13239	VBD	denotes	had
T3067	13240-13241	DT	denotes	a
T3069	13242-13243	CD	denotes	5
T3071	13243-13244	HYPH	denotes	-
T3072	13245-13247	IN	denotes	to
T3070	13248-13249	CD	denotes	6
T3074	13249-13250	HYPH	denotes	-
T3073	13250-13254	JJ	denotes	fold
T3068	13255-13264	NN	denotes	elevation
T3076	13265-13267	IN	denotes	of
T3077	13268-13273	NN	denotes	serum
T3078	13274-13291	NN	denotes	decenoylcarnitine
T3075	13292-13299	JJ	denotes	evident
T3079	13300-13302	IN	denotes	in
T3080	13303-13306	DT	denotes	the
T3082	13307-13320	NN	denotes	acylcarnitine
T3081	13321-13328	NN	denotes	profile
T3083	13329-13330	-LRB-	denotes	(
T3084	13330-13336	NN	denotes	Figure
T3085	13337-13339	CD	denotes	4A
T3086	13339-13340	-RRB-	denotes	)
T3087	13340-13341	.	denotes	.
T3088	13341-13435	sentence	denotes	Bile acylcarnitine analysis revealed a similar acylcarnitine pattern as in serum (Figure 4B).
T3089	13342-13346	NN	denotes	Bile
T3090	13347-13360	NN	denotes	acylcarnitine
T3091	13361-13369	NN	denotes	analysis
T3092	13370-13378	VBD	denotes	revealed
T3093	13379-13380	DT	denotes	a
T3095	13381-13388	JJ	denotes	similar
T3096	13389-13402	NN	denotes	acylcarnitine
T3094	13403-13410	NN	denotes	pattern
T3097	13411-13413	IN	denotes	as
T3098	13414-13416	IN	denotes	in
T3099	13417-13422	NN	denotes	serum
T3100	13423-13424	-LRB-	denotes	(
T3101	13424-13430	NN	denotes	Figure
T3102	13431-13433	CD	denotes	4B
T3103	13433-13434	-RRB-	denotes	)
T3104	13434-13435	.	denotes	.
T3105	13435-13562	sentence	denotes	However, the acylcarnitine profiles of the MCAD−/− mice are different from those of human MCAD-deficient patients (Figure 4C).
T3106	13436-13443	RB	denotes	However
T3108	13443-13445	,	denotes	,
T3109	13445-13448	DT	denotes	the
T3111	13449-13462	NN	denotes	acylcarnitine
T3110	13463-13471	NNS	denotes	profiles
T3112	13472-13474	IN	denotes	of
T3113	13475-13478	DT	denotes	the
T3115	13479-13483	NN	denotes	MCAD
T3116	13483-13484	SYM	denotes	−
T3117	13484-13485	HYPH	denotes	/
T3118	13485-13486	SYM	denotes	−
T3114	13487-13491	NNS	denotes	mice
T3107	13492-13495	VBP	denotes	are
T3119	13496-13505	JJ	denotes	different
T3120	13506-13510	IN	denotes	from
T3121	13511-13516	DT	denotes	those
T3122	13517-13519	IN	denotes	of
T3123	13520-13525	JJ	denotes	human
T3125	13526-13530	NN	denotes	MCAD
T3127	13530-13531	HYPH	denotes	-
T3126	13531-13540	JJ	denotes	deficient
T3124	13541-13549	NNS	denotes	patients
T3128	13550-13551	-LRB-	denotes	(
T3129	13551-13557	NN	denotes	Figure
T3130	13558-13560	CD	denotes	4C
T3131	13560-13561	-RRB-	denotes	)
T3132	13561-13562	.	denotes	.
T3133	13562-13778	sentence	denotes	Human patients present with elevated levels of C6, C8, and C10:1 acylcarnitines, as did the mutant mice; however, the predominant peak was C8 acylcarnitine in humans, whereas in the mouse it was C10:1 acylcarnitine.
T3134	13563-13568	JJ	denotes	Human
T3135	13569-13577	NNS	denotes	patients
T3136	13578-13585	VBP	denotes	present
T3138	13586-13590	IN	denotes	with
T3139	13591-13599	VBN	denotes	elevated
T3140	13600-13606	NNS	denotes	levels
T3141	13607-13609	IN	denotes	of
T3142	13610-13612	NN	denotes	C6
T3143	13612-13614	,	denotes	,
T3144	13614-13616	NN	denotes	C8
T3145	13616-13618	,	denotes	,
T3146	13618-13621	CC	denotes	and
T3147	13622-13625	NN	denotes	C10
T3148	13625-13626	:	denotes	:
T3149	13626-13627	CD	denotes	1
T3150	13628-13642	NNS	denotes	acylcarnitines
T3151	13642-13644	,	denotes	,
T3152	13644-13646	IN	denotes	as
T3153	13647-13650	VBD	denotes	did
T3154	13651-13654	DT	denotes	the
T3156	13655-13661	NN	denotes	mutant
T3155	13662-13666	NNS	denotes	mice
T3157	13666-13667	:	denotes	;
T3158	13668-13675	RB	denotes	however
T3159	13675-13677	,	denotes	,
T3160	13677-13680	DT	denotes	the
T3162	13681-13692	JJ	denotes	predominant
T3161	13693-13697	NN	denotes	peak
T3137	13698-13701	VBD	denotes	was
T3163	13702-13704	NN	denotes	C8
T3164	13705-13718	NN	denotes	acylcarnitine
T3165	13719-13721	IN	denotes	in
T3166	13722-13728	NNS	denotes	humans
T3167	13728-13730	,	denotes	,
T3168	13730-13737	IN	denotes	whereas
T3170	13738-13740	IN	denotes	in
T3171	13741-13744	DT	denotes	the
T3172	13745-13750	NN	denotes	mouse
T3173	13751-13753	PRP	denotes	it
T3169	13754-13757	VBD	denotes	was
T3174	13758-13761	NN	denotes	C10
T3176	13761-13762	:	denotes	:
T3177	13762-13763	CD	denotes	1
T3175	13764-13777	NN	denotes	acylcarnitine
T3178	13777-13778	.	denotes	.
T7814	13789-13802	NN	denotes	Acylcarnitine
T7815	13803-13811	NNS	denotes	Analyses
T7816	13811-13888	sentence	denotes	(A) Serum acylcarnitine analysis of MCAD+/+ (n = 4) and MCAD−/− mice (n = 4)
T7817	13812-13813	-LRB-	denotes	(
T7818	13813-13814	LS	denotes	A
T7820	13814-13815	-RRB-	denotes	)
T7821	13816-13821	NN	denotes	Serum
T7822	13822-13835	NN	denotes	acylcarnitine
T7819	13836-13844	NN	denotes	analysis
T7823	13845-13847	IN	denotes	of
T7824	13848-13852	NN	denotes	MCAD
T7826	13852-13853	SYM	denotes	+
T7827	13853-13854	HYPH	denotes	/
T7828	13854-13855	SYM	denotes	+
T7829	13856-13857	-LRB-	denotes	(
T7831	13857-13858	NN	denotes	n
T7832	13859-13860	SYM	denotes	=
T7830	13861-13862	CD	denotes	4
T7833	13862-13863	-RRB-	denotes	)
T7834	13864-13867	CC	denotes	and
T7835	13868-13872	NN	denotes	MCAD
T7836	13872-13873	SYM	denotes	−
T7837	13873-13874	HYPH	denotes	/
T7838	13874-13875	SYM	denotes	−
T7825	13876-13880	NNS	denotes	mice
T7839	13881-13882	-LRB-	denotes	(
T7841	13882-13883	NN	denotes	n
T7842	13884-13885	SYM	denotes	=
T7840	13886-13887	CD	denotes	4
T7843	13887-13888	-RRB-	denotes	)
T7844	13888-13976	sentence	denotes	There are significant elevations in acylcarnitine species as indicated in MCAD−/− mice.
T7845	13889-13894	EX	denotes	There
T7846	13895-13898	VBP	denotes	are
T7847	13899-13910	JJ	denotes	significant
T7848	13911-13921	NNS	denotes	elevations
T7849	13922-13924	IN	denotes	in
T7850	13925-13938	NN	denotes	acylcarnitine
T7851	13939-13946	NNS	denotes	species
T7852	13947-13949	IN	denotes	as
T7853	13950-13959	VBN	denotes	indicated
T7854	13960-13962	IN	denotes	in
T7855	13963-13967	NN	denotes	MCAD
T7857	13967-13968	SYM	denotes	−
T7858	13968-13969	HYPH	denotes	/
T7859	13969-13970	SYM	denotes	−
T7856	13971-13975	NNS	denotes	mice
T7860	13975-13976	.	denotes	.
T7861	13976-14032	sentence	denotes	Values shown are mean values ± standard deviation (SD).
T7862	13977-13983	NNS	denotes	Values
T7864	13984-13989	VBN	denotes	shown
T7863	13990-13993	VBP	denotes	are
T7865	13994-13998	NN	denotes	mean
T7866	13999-14005	NNS	denotes	values
T7867	14006-14007	SYM	denotes	±
T7868	14008-14016	JJ	denotes	standard
T7869	14017-14026	NN	denotes	deviation
T7870	14027-14028	-LRB-	denotes	(
T7871	14028-14030	NN	denotes	SD
T7872	14030-14031	-RRB-	denotes	)
T7873	14031-14032	.	denotes	.
T7874	14032-14087	sentence	denotes	Asterisk indicates p < 0.002 and ‡ indicates p < 0.01.
T7875	14033-14041	NN	denotes	Asterisk
T7876	14042-14051	VBZ	denotes	indicates
T7877	14052-14053	NN	denotes	p
T7878	14054-14055	SYM	denotes	<
T7879	14056-14061	CD	denotes	0.002
T7880	14062-14065	CC	denotes	and
T7881	14066-14067	SYM	denotes	‡
T7882	14068-14077	VBZ	denotes	indicates
T7883	14078-14079	NN	denotes	p
T7884	14080-14081	SYM	denotes	<
T7885	14082-14086	CD	denotes	0.01
T7886	14086-14087	.	denotes	.
T7887	14087-14191	sentence	denotes	(B) There are significant elevations in bile acylcarnitines of the same mice shown in (A) as indicated.
T7888	14088-14089	-LRB-	denotes	(
T7889	14089-14090	LS	denotes	B
T7891	14090-14091	-RRB-	denotes	)
T7892	14092-14097	EX	denotes	There
T7890	14098-14101	VBP	denotes	are
T7893	14102-14113	JJ	denotes	significant
T7894	14114-14124	NNS	denotes	elevations
T7895	14125-14127	IN	denotes	in
T7896	14128-14132	NN	denotes	bile
T7897	14133-14147	NNS	denotes	acylcarnitines
T7898	14148-14150	IN	denotes	of
T7899	14151-14154	DT	denotes	the
T7901	14155-14159	JJ	denotes	same
T7900	14160-14164	NNS	denotes	mice
T7902	14165-14170	VBN	denotes	shown
T7903	14171-14173	IN	denotes	in
T7904	14174-14175	-LRB-	denotes	(
T7905	14175-14176	NN	denotes	A
T7906	14176-14177	-RRB-	denotes	)
T7907	14178-14180	IN	denotes	as
T7908	14181-14190	VBN	denotes	indicated
T7909	14190-14191	.	denotes	.
T7910	14191-14226	sentence	denotes	Values shown are mean values ± SD.
T7911	14192-14198	NNS	denotes	Values
T7913	14199-14204	VBN	denotes	shown
T7912	14205-14208	VBP	denotes	are
T7914	14209-14213	NN	denotes	mean
T7915	14214-14220	NNS	denotes	values
T7916	14221-14222	SYM	denotes	±
T7917	14223-14225	NN	denotes	SD
T7918	14225-14226	.	denotes	.
T7919	14226-14256	sentence	denotes	Asterisk indicates p < 0.001.
T7920	14227-14235	NN	denotes	Asterisk
T7921	14236-14245	VBZ	denotes	indicates
T7922	14246-14247	NN	denotes	p
T7923	14248-14249	SYM	denotes	<
T7924	14250-14255	CD	denotes	0.001
T7925	14255-14256	.	denotes	.
T7926	14256-14357	sentence	denotes	(C) Bile acylcarnitine profile of an MCAD−/− mouse compared to a human patient with MCAD deficiency.
T7927	14257-14258	-LRB-	denotes	(
T7928	14258-14259	LS	denotes	C
T7930	14259-14260	-RRB-	denotes	)
T7931	14261-14265	NN	denotes	Bile
T7932	14266-14279	NN	denotes	acylcarnitine
T7929	14280-14287	NN	denotes	profile
T7933	14288-14290	IN	denotes	of
T7934	14291-14293	DT	denotes	an
T7936	14294-14298	NN	denotes	MCAD
T7937	14298-14299	SYM	denotes	−
T7938	14299-14300	HYPH	denotes	/
T7939	14300-14301	SYM	denotes	−
T7935	14302-14307	NN	denotes	mouse
T7940	14308-14316	VBN	denotes	compared
T7941	14317-14319	IN	denotes	to
T7942	14320-14321	DT	denotes	a
T7944	14322-14327	JJ	denotes	human
T7943	14328-14335	NN	denotes	patient
T7945	14336-14340	IN	denotes	with
T7946	14341-14345	NN	denotes	MCAD
T7947	14346-14356	NN	denotes	deficiency
T7948	14356-14357	.	denotes	.
T7949	14357-14406	sentence	denotes	Internal standards are indicated by an asterisk.
T7950	14358-14366	JJ	denotes	Internal
T7951	14367-14376	NNS	denotes	standards
T7953	14377-14380	VBP	denotes	are
T7952	14381-14390	VBN	denotes	indicated
T7954	14391-14393	IN	denotes	by
T7955	14394-14396	DT	denotes	an
T7956	14397-14405	NN	denotes	asterisk
T7957	14405-14406	.	denotes	.
T3317	14408-14422	NN	denotes	Histopathology
T3318	14422-14692	sentence	denotes	Complete histopathologic examination of one group of mutant and MCAD+/+ control mice after a 24-h fast demonstrated diffuse microvesicular and macrovesicular hepatic steatosis in 6–8-wk-old MCAD−/− mice whereas MCAD+/+ mice had no histologic changes (Figure 5A and 5B).
T3319	14423-14431	JJ	denotes	Complete
T3321	14432-14447	JJ	denotes	histopathologic
T3320	14448-14459	NN	denotes	examination
T3323	14460-14462	IN	denotes	of
T3324	14463-14466	CD	denotes	one
T3325	14467-14472	NN	denotes	group
T3326	14473-14475	IN	denotes	of
T3327	14476-14482	NN	denotes	mutant
T3329	14483-14486	CC	denotes	and
T3330	14487-14491	NN	denotes	MCAD
T3331	14491-14492	SYM	denotes	+
T3332	14492-14493	HYPH	denotes	/
T3333	14493-14494	SYM	denotes	+
T3334	14495-14502	NN	denotes	control
T3328	14503-14507	NNS	denotes	mice
T3335	14508-14513	IN	denotes	after
T3336	14514-14515	DT	denotes	a
T3338	14516-14518	CD	denotes	24
T3340	14518-14519	HYPH	denotes	-
T3339	14519-14520	NN	denotes	h
T3337	14521-14525	NN	denotes	fast
T3322	14526-14538	VBD	denotes	demonstrated
T3341	14539-14546	JJ	denotes	diffuse
T3343	14547-14561	JJ	denotes	microvesicular
T3344	14562-14565	CC	denotes	and
T3345	14566-14580	JJ	denotes	macrovesicular
T3346	14581-14588	JJ	denotes	hepatic
T3342	14589-14598	NN	denotes	steatosis
T3347	14599-14601	IN	denotes	in
T3348	14602-14603	CD	denotes	6
T3350	14603-14604	SYM	denotes	–
T3349	14604-14605	CD	denotes	8
T3352	14605-14606	HYPH	denotes	-
T3351	14606-14608	NN	denotes	wk
T3354	14608-14609	HYPH	denotes	-
T3353	14609-14612	JJ	denotes	old
T3356	14613-14617	NN	denotes	MCAD
T3357	14617-14618	SYM	denotes	−
T3358	14618-14619	HYPH	denotes	/
T3359	14619-14620	SYM	denotes	−
T3355	14621-14625	NNS	denotes	mice
T3360	14626-14633	IN	denotes	whereas
T3362	14634-14638	NN	denotes	MCAD
T3364	14638-14639	SYM	denotes	+
T3365	14639-14640	HYPH	denotes	/
T3366	14640-14641	SYM	denotes	+
T3363	14642-14646	NNS	denotes	mice
T3361	14647-14650	VBD	denotes	had
T3367	14651-14653	DT	denotes	no
T3369	14654-14664	JJ	denotes	histologic
T3368	14665-14672	NNS	denotes	changes
T3370	14673-14674	-LRB-	denotes	(
T3372	14674-14680	NN	denotes	Figure
T3371	14681-14683	CD	denotes	5A
T3373	14684-14687	CC	denotes	and
T3374	14688-14690	CD	denotes	5B
T3375	14690-14691	-RRB-	denotes	)
T3376	14691-14692	.	denotes	.
T3377	14692-14827	sentence	denotes	In another group of 4-wk-old MCAD+/+ and MCAD−/− mice fasted for 24-h, there were minimal to no abnormalities in all organs evaluated.
T3378	14693-14695	IN	denotes	In
T3380	14696-14703	DT	denotes	another
T3381	14704-14709	NN	denotes	group
T3382	14710-14712	IN	denotes	of
T3383	14713-14714	CD	denotes	4
T3385	14714-14715	HYPH	denotes	-
T3384	14715-14717	NN	denotes	wk
T3387	14717-14718	HYPH	denotes	-
T3386	14718-14721	JJ	denotes	old
T3389	14722-14726	NN	denotes	MCAD
T3390	14726-14727	SYM	denotes	+
T3391	14727-14728	HYPH	denotes	/
T3392	14728-14729	SYM	denotes	+
T3393	14730-14733	CC	denotes	and
T3394	14734-14738	NN	denotes	MCAD
T3395	14738-14739	SYM	denotes	−
T3396	14739-14740	HYPH	denotes	/
T3397	14740-14741	SYM	denotes	−
T3388	14742-14746	NNS	denotes	mice
T3398	14747-14753	VBN	denotes	fasted
T3399	14754-14757	IN	denotes	for
T3400	14758-14760	CD	denotes	24
T3402	14760-14761	HYPH	denotes	-
T3401	14761-14762	NN	denotes	h
T3403	14762-14764	,	denotes	,
T3404	14764-14769	EX	denotes	there
T3379	14770-14774	VBD	denotes	were
T3405	14775-14782	JJ	denotes	minimal
T3406	14783-14785	IN	denotes	to
T3407	14786-14788	DT	denotes	no
T3408	14789-14802	NNS	denotes	abnormalities
T3409	14803-14805	IN	denotes	in
T3410	14806-14809	DT	denotes	all
T3411	14810-14816	NNS	denotes	organs
T3412	14817-14826	VBN	denotes	evaluated
T3413	14826-14827	.	denotes	.
T3414	14827-14899	sentence	denotes	Only the older MCAD−/− mice, therefore, demonstrated hepatic steatosis.
T3415	14828-14832	RB	denotes	Only
T3417	14833-14836	DT	denotes	the
T3418	14837-14842	JJR	denotes	older
T3419	14843-14847	NN	denotes	MCAD
T3420	14847-14848	SYM	denotes	−
T3421	14848-14849	HYPH	denotes	/
T3422	14849-14850	SYM	denotes	−
T3416	14851-14855	NNS	denotes	mice
T3424	14855-14857	,	denotes	,
T3425	14857-14866	RB	denotes	therefore
T3426	14866-14868	,	denotes	,
T3423	14868-14880	VBD	denotes	demonstrated
T3427	14881-14888	JJ	denotes	hepatic
T3428	14889-14898	NN	denotes	steatosis
T3429	14898-14899	.	denotes	.
T3430	14899-14972	sentence	denotes	In addition, we found sporadic cardiac lesions in multiple MCAD−/− mice.
T3431	14900-14902	IN	denotes	In
T3433	14903-14911	NN	denotes	addition
T3434	14911-14913	,	denotes	,
T3435	14913-14915	PRP	denotes	we
T3432	14916-14921	VBD	denotes	found
T3436	14922-14930	JJ	denotes	sporadic
T3438	14931-14938	JJ	denotes	cardiac
T3437	14939-14946	NNS	denotes	lesions
T3439	14947-14949	IN	denotes	in
T3440	14950-14958	JJ	denotes	multiple
T3442	14959-14963	NN	denotes	MCAD
T3443	14963-14964	SYM	denotes	−
T3444	14964-14965	HYPH	denotes	/
T3445	14965-14966	SYM	denotes	−
T3441	14967-14971	NNS	denotes	mice
T3446	14971-14972	.	denotes	.
T3447	14972-15588	sentence	denotes	Figure 5 Histopathology of MCAD+/+ and MCAD−/− Mice (A) MCAD+/+ mice had no evidence of hepatic steatosis following a 24-h fast. Liver section with Oil-Red O stain. (B) Hepatosteatosis in MCAD−/− mouse following a 24-h fast. Oil-Red O stained liver sections revealed severe and diffuse microvesicular and macrovesicular hepatic steatosis in MCAD−/− mice. (C and D) Diffuse cardiomyopathy with chronic active multifocal myocyte degeneration and necrosis in MCAD−/− mice. In one example, an MCAD−/− mouse had diffuse cardiomyopathy with chronic active multifocal myocyte degeneration and necrosis (Figure 5C and 5D).
T8009	14983-14997	NN	denotes	Histopathology
T8010	14998-15000	IN	denotes	of
T8011	15001-15005	NN	denotes	MCAD
T8013	15005-15006	SYM	denotes	+
T8014	15006-15007	HYPH	denotes	/
T8015	15007-15008	SYM	denotes	+
T8016	15009-15012	CC	denotes	and
T8017	15013-15017	NN	denotes	MCAD
T8018	15017-15018	SYM	denotes	−
T8019	15018-15019	HYPH	denotes	/
T8020	15019-15020	SYM	denotes	−
T8012	15021-15025	NNS	denotes	Mice
T8021	15025-15102	sentence	denotes	(A) MCAD+/+ mice had no evidence of hepatic steatosis following a 24-h fast.
T8022	15026-15027	-LRB-	denotes	(
T8023	15027-15028	LS	denotes	A
T8025	15028-15029	-RRB-	denotes	)
T8026	15030-15034	NN	denotes	MCAD
T8028	15034-15035	SYM	denotes	+
T8029	15035-15036	HYPH	denotes	/
T8030	15036-15037	SYM	denotes	+
T8027	15038-15042	NNS	denotes	mice
T8024	15043-15046	VBD	denotes	had
T8031	15047-15049	DT	denotes	no
T8032	15050-15058	NN	denotes	evidence
T8033	15059-15061	IN	denotes	of
T8034	15062-15069	JJ	denotes	hepatic
T8035	15070-15079	NN	denotes	steatosis
T8036	15080-15089	VBG	denotes	following
T8037	15090-15091	DT	denotes	a
T8039	15092-15094	CD	denotes	24
T8041	15094-15095	HYPH	denotes	-
T8040	15095-15096	NN	denotes	h
T8038	15097-15101	NN	denotes	fast
T8042	15101-15102	.	denotes	.
T8043	15102-15138	sentence	denotes	Liver section with Oil-Red O stain.
T8044	15103-15108	NN	denotes	Liver
T8045	15109-15116	NN	denotes	section
T8046	15117-15121	IN	denotes	with
T8047	15122-15125	NN	denotes	Oil
T8049	15125-15126	HYPH	denotes	-
T8050	15126-15129	JJ	denotes	Red
T8048	15130-15131	NN	denotes	O
T8051	15132-15137	NN	denotes	stain
T8052	15137-15138	.	denotes	.
T8053	15138-15198	sentence	denotes	(B) Hepatosteatosis in MCAD−/− mouse following a 24-h fast.
T8054	15139-15140	-LRB-	denotes	(
T8055	15140-15141	LS	denotes	B
T8057	15141-15142	-RRB-	denotes	)
T8056	15143-15158	NN	denotes	Hepatosteatosis
T8058	15159-15161	IN	denotes	in
T8059	15162-15166	NN	denotes	MCAD
T8061	15166-15167	NN	denotes	−
T8062	15167-15168	HYPH	denotes	/
T8060	15168-15169	NN	denotes	−
T8063	15170-15175	NN	denotes	mouse
T8064	15176-15185	VBG	denotes	following
T8065	15186-15187	DT	denotes	a
T8067	15188-15190	CD	denotes	24
T8069	15190-15191	HYPH	denotes	-
T8068	15191-15192	NN	denotes	h
T8066	15193-15197	NN	denotes	fast
T8070	15197-15198	.	denotes	.
T8071	15198-15328	sentence	denotes	Oil-Red O stained liver sections revealed severe and diffuse microvesicular and macrovesicular hepatic steatosis in MCAD−/− mice.
T8072	15199-15202	NN	denotes	Oil
T8074	15202-15203	HYPH	denotes	-
T8075	15203-15206	JJ	denotes	Red
T8073	15207-15208	NN	denotes	O
T8076	15209-15216	VBN	denotes	stained
T8078	15217-15222	NN	denotes	liver
T8077	15223-15231	NNS	denotes	sections
T8079	15232-15240	VBD	denotes	revealed
T8080	15241-15247	JJ	denotes	severe
T8082	15248-15251	CC	denotes	and
T8083	15252-15259	JJ	denotes	diffuse
T8084	15260-15274	JJ	denotes	microvesicular
T8085	15275-15278	CC	denotes	and
T8086	15279-15293	JJ	denotes	macrovesicular
T8087	15294-15301	JJ	denotes	hepatic
T8081	15302-15311	NN	denotes	steatosis
T8088	15312-15314	IN	denotes	in
T8089	15315-15319	NN	denotes	MCAD
T8091	15319-15320	SYM	denotes	−
T8092	15320-15321	HYPH	denotes	/
T8093	15321-15322	SYM	denotes	−
T8090	15323-15327	NNS	denotes	mice
T8094	15327-15328	.	denotes	.
T8095	15328-15443	sentence	denotes	(C and D) Diffuse cardiomyopathy with chronic active multifocal myocyte degeneration and necrosis in MCAD−/− mice.
T8096	15329-15330	-LRB-	denotes	(
T8097	15330-15331	LS	denotes	C
T8099	15332-15335	CC	denotes	and
T8100	15336-15337	LS	denotes	D
T8101	15337-15338	-RRB-	denotes	)
T8102	15339-15346	JJ	denotes	Diffuse
T8098	15347-15361	NN	denotes	cardiomyopathy
T8103	15362-15366	IN	denotes	with
T8104	15367-15374	JJ	denotes	chronic
T8106	15375-15381	JJ	denotes	active
T8107	15382-15392	JJ	denotes	multifocal
T8108	15393-15400	NN	denotes	myocyte
T8105	15401-15413	NN	denotes	degeneration
T8109	15414-15417	CC	denotes	and
T8110	15418-15426	NN	denotes	necrosis
T8111	15427-15429	IN	denotes	in
T8112	15430-15434	NN	denotes	MCAD
T8114	15434-15435	SYM	denotes	−
T8115	15435-15436	HYPH	denotes	/
T8116	15436-15437	SYM	denotes	−
T8113	15438-15442	NNS	denotes	mice
T8117	15442-15443	.	denotes	.
T3448	15444-15446	IN	denotes	In
T3450	15447-15450	CD	denotes	one
T3451	15451-15458	NN	denotes	example
T3452	15458-15460	,	denotes	,
T3453	15460-15462	DT	denotes	an
T3455	15463-15467	NN	denotes	MCAD
T3456	15467-15468	SYM	denotes	−
T3457	15468-15469	HYPH	denotes	/
T3458	15469-15470	SYM	denotes	−
T3454	15471-15476	NN	denotes	mouse
T3449	15477-15480	VBD	denotes	had
T3459	15481-15488	JJ	denotes	diffuse
T3460	15489-15503	NN	denotes	cardiomyopathy
T3461	15504-15508	IN	denotes	with
T3462	15509-15516	JJ	denotes	chronic
T3464	15517-15523	JJ	denotes	active
T3465	15524-15534	JJ	denotes	multifocal
T3466	15535-15542	NN	denotes	myocyte
T3463	15543-15555	NN	denotes	degeneration
T3467	15556-15559	CC	denotes	and
T3468	15560-15568	NN	denotes	necrosis
T3469	15569-15570	-LRB-	denotes	(
T3471	15570-15576	NN	denotes	Figure
T3470	15577-15579	CD	denotes	5C
T3472	15580-15583	CC	denotes	and
T3473	15584-15586	CD	denotes	5D
T3474	15586-15587	-RRB-	denotes	)
T3475	15587-15588	.	denotes	.
T3476	15588-15761	sentence	denotes	Changes in degenerating myocytes included swelling, pale staining, and, in portions of the sarcoplasm, replacement of myofibrils with finely granular eosinophilic material.
T3477	15589-15596	NNS	denotes	Changes
T3479	15597-15599	IN	denotes	in
T3480	15600-15612	VBG	denotes	degenerating
T3481	15613-15621	NNS	denotes	myocytes
T3478	15622-15630	VBD	denotes	included
T3482	15631-15639	VBG	denotes	swelling
T3483	15639-15641	,	denotes	,
T3484	15641-15645	JJ	denotes	pale
T3485	15646-15654	NN	denotes	staining
T3486	15654-15656	,	denotes	,
T3487	15656-15659	CC	denotes	and
T3488	15659-15661	,	denotes	,
T3489	15661-15663	IN	denotes	in
T3491	15664-15672	NNS	denotes	portions
T3492	15673-15675	IN	denotes	of
T3493	15676-15679	DT	denotes	the
T3494	15680-15690	NN	denotes	sarcoplasm
T3495	15690-15692	,	denotes	,
T3490	15692-15703	NN	denotes	replacement
T3496	15704-15706	IN	denotes	of
T3497	15707-15717	NNS	denotes	myofibrils
T3498	15718-15722	IN	denotes	with
T3499	15723-15729	RB	denotes	finely
T3500	15730-15738	JJ	denotes	granular
T3502	15739-15751	JJ	denotes	eosinophilic
T3501	15752-15760	NN	denotes	material
T3503	15760-15761	.	denotes	.
T3504	15761-15849	sentence	denotes	Nuclei of affected myocytes were large, pale, and vesicular and had prominent nucleoli.
T3505	15762-15768	NNS	denotes	Nuclei
T3507	15769-15771	IN	denotes	of
T3508	15772-15780	VBN	denotes	affected
T3509	15781-15789	NNS	denotes	myocytes
T3506	15790-15794	VBD	denotes	were
T3510	15795-15800	JJ	denotes	large
T3511	15800-15802	,	denotes	,
T3512	15802-15806	JJ	denotes	pale
T3513	15806-15808	,	denotes	,
T3514	15808-15811	CC	denotes	and
T3515	15812-15821	JJ	denotes	vesicular
T3516	15822-15825	CC	denotes	and
T3517	15826-15829	VBD	denotes	had
T3518	15830-15839	JJ	denotes	prominent
T3519	15840-15848	NNS	denotes	nucleoli
T3520	15848-15849	.	denotes	.
T3521	15849-15938	sentence	denotes	In the most severely affected areas, there was loss of myocytes accompanied by fibrosis.
T3522	15850-15852	IN	denotes	In
T3524	15853-15856	DT	denotes	the
T3526	15857-15861	RBS	denotes	most
T3527	15862-15870	RB	denotes	severely
T3528	15871-15879	VBN	denotes	affected
T3525	15880-15885	NNS	denotes	areas
T3529	15885-15887	,	denotes	,
T3530	15887-15892	EX	denotes	there
T3523	15893-15896	VBD	denotes	was
T3531	15897-15901	NN	denotes	loss
T3532	15902-15904	IN	denotes	of
T3533	15905-15913	NNS	denotes	myocytes
T3534	15914-15925	VBN	denotes	accompanied
T3535	15926-15928	IN	denotes	by
T3536	15929-15937	NN	denotes	fibrosis
T3537	15937-15938	.	denotes	.
T3538	15938-16165	sentence	denotes	In the wall of the aorta at the base of the heart there was multifocal degeneration of the elastic tissue, accompanied by multifocal collections of globular translucent yellow-brown pigment interpreted to be ceroid/lipofuscin.
T3539	15939-15941	IN	denotes	In
T3541	15942-15945	DT	denotes	the
T3542	15946-15950	NN	denotes	wall
T3543	15951-15953	IN	denotes	of
T3544	15954-15957	DT	denotes	the
T3545	15958-15963	NN	denotes	aorta
T3546	15964-15966	IN	denotes	at
T3547	15967-15970	DT	denotes	the
T3548	15971-15975	NN	denotes	base
T3549	15976-15978	IN	denotes	of
T3550	15979-15982	DT	denotes	the
T3551	15983-15988	NN	denotes	heart
T3552	15989-15994	EX	denotes	there
T3540	15995-15998	VBD	denotes	was
T3553	15999-16009	JJ	denotes	multifocal
T3554	16010-16022	NN	denotes	degeneration
T3555	16023-16025	IN	denotes	of
T3556	16026-16029	DT	denotes	the
T3558	16030-16037	JJ	denotes	elastic
T3557	16038-16044	NN	denotes	tissue
T3559	16044-16046	,	denotes	,
T3560	16046-16057	VBN	denotes	accompanied
T3561	16058-16060	IN	denotes	by
T3562	16061-16071	JJ	denotes	multifocal
T3563	16072-16083	NNS	denotes	collections
T3564	16084-16086	IN	denotes	of
T3565	16087-16095	JJ	denotes	globular
T3567	16096-16107	JJ	denotes	translucent
T3568	16108-16114	JJ	denotes	yellow
T3570	16114-16115	HYPH	denotes	-
T3569	16115-16120	JJ	denotes	brown
T3566	16121-16128	NN	denotes	pigment
T3571	16129-16140	VBN	denotes	interpreted
T3572	16141-16143	TO	denotes	to
T3573	16144-16146	VB	denotes	be
T3574	16147-16153	NN	denotes	ceroid
T3576	16153-16154	HYPH	denotes	/
T3575	16154-16164	NN	denotes	lipofuscin
T3577	16164-16165	.	denotes	.
T3578	16165-16229	sentence	denotes	Similar deposits were scattered within adjacent adipose tissue.
T3579	16166-16173	JJ	denotes	Similar
T3580	16174-16182	NNS	denotes	deposits
T3582	16183-16187	VBD	denotes	were
T3581	16188-16197	VBN	denotes	scattered
T3583	16198-16204	IN	denotes	within
T3584	16205-16213	JJ	denotes	adjacent
T3586	16214-16221	NN	denotes	adipose
T3585	16222-16228	NN	denotes	tissue
T3587	16228-16229	.	denotes	.
T3809	16242-16254	RB	denotes	Successfully
T3810	16255-16264	VBG	denotes	targeting
T3812	16265-16270	NN	denotes	Acadm
T3811	16271-16279	VBD	denotes	produced
T3813	16280-16281	DT	denotes	a
T3815	16282-16287	NN	denotes	mouse
T3814	16288-16293	NN	denotes	model
T3816	16294-16297	IN	denotes	for
T3817	16298-16302	NN	denotes	MCAD
T3818	16303-16313	NN	denotes	deficiency
T3819	16314-16318	IN	denotes	with
T3820	16319-16327	NNS	denotes	features
T3821	16328-16332	WDT	denotes	that
T3822	16333-16338	VBP	denotes	mimic
T3823	16339-16342	DT	denotes	the
T3825	16343-16351	JJ	denotes	clinical
T3826	16351-16353	,	denotes	,
T3827	16353-16364	JJ	denotes	biochemical
T3828	16364-16366	,	denotes	,
T3829	16366-16369	CC	denotes	and
T3830	16370-16380	JJ	denotes	pathologic
T3824	16381-16390	NN	denotes	phenotype
T3831	16391-16396	VBN	denotes	found
T3832	16397-16399	IN	denotes	in
T3833	16400-16405	JJ	denotes	human
T3834	16406-16414	NNS	denotes	patients
T3835	16414-16415	.	denotes	.
T3836	16415-16542	sentence	denotes	MCAD-deficient patients have abnormal plasma and urine metabolites associated with the medium chain–length enzyme specificity.
T3837	16416-16420	NN	denotes	MCAD
T3839	16420-16421	HYPH	denotes	-
T3838	16421-16430	JJ	denotes	deficient
T3840	16431-16439	NNS	denotes	patients
T3841	16440-16444	VBP	denotes	have
T3842	16445-16453	JJ	denotes	abnormal
T3844	16454-16460	NN	denotes	plasma
T3845	16461-16464	CC	denotes	and
T3846	16465-16470	NN	denotes	urine
T3843	16471-16482	NNS	denotes	metabolites
T3847	16483-16493	VBN	denotes	associated
T3848	16494-16498	IN	denotes	with
T3849	16499-16502	DT	denotes	the
T3851	16503-16509	JJ	denotes	medium
T3852	16510-16515	NN	denotes	chain
T3854	16515-16516	HYPH	denotes	–
T3853	16516-16522	NN	denotes	length
T3855	16523-16529	NN	denotes	enzyme
T3850	16530-16541	NN	denotes	specificity
T3856	16541-16542	.	denotes	.
T3857	16542-16661	sentence	denotes	MCAD-deficient patients [15] often display a characteristic urinary hexanoylglycine peak, as was seen in MCAD−/− mice.
T3858	16543-16547	NN	denotes	MCAD
T3860	16547-16548	HYPH	denotes	-
T3859	16548-16557	JJ	denotes	deficient
T3861	16558-16566	NNS	denotes	patients
T3863	16567-16568	-LRB-	denotes	[
T3864	16568-16570	CD	denotes	15
T3865	16570-16571	-RRB-	denotes	]
T3866	16572-16577	RB	denotes	often
T3862	16578-16585	VBP	denotes	display
T3867	16586-16587	DT	denotes	a
T3869	16588-16602	JJ	denotes	characteristic
T3870	16603-16610	JJ	denotes	urinary
T3871	16611-16626	NN	denotes	hexanoylglycine
T3868	16627-16631	NN	denotes	peak
T3872	16631-16633	,	denotes	,
T3873	16633-16635	IN	denotes	as
T3875	16636-16639	VBD	denotes	was
T3874	16640-16644	VBN	denotes	seen
T3876	16645-16647	IN	denotes	in
T3877	16648-16652	NN	denotes	MCAD
T3879	16652-16653	SYM	denotes	−
T3880	16653-16654	HYPH	denotes	/
T3881	16654-16655	SYM	denotes	−
T3878	16656-16660	NNS	denotes	mice
T3882	16660-16661	.	denotes	.
T3883	16661-16789	sentence	denotes	Acylcarnitine analysis indicated, however, mouse MCAD is more active toward longer chain substrates than the human MCAD enzyme.
T3884	16662-16675	NN	denotes	Acylcarnitine
T3885	16676-16684	NN	denotes	analysis
T3886	16685-16694	VBD	denotes	indicated
T3887	16694-16696	,	denotes	,
T3888	16696-16703	RB	denotes	however
T3889	16703-16705	,	denotes	,
T3890	16705-16710	NN	denotes	mouse
T3891	16711-16715	NN	denotes	MCAD
T3892	16716-16718	VBZ	denotes	is
T3893	16719-16723	RBR	denotes	more
T3894	16724-16730	JJ	denotes	active
T3895	16731-16737	IN	denotes	toward
T3896	16738-16744	JJR	denotes	longer
T3898	16745-16750	NN	denotes	chain
T3897	16751-16761	NNS	denotes	substrates
T3899	16762-16766	IN	denotes	than
T3900	16767-16770	DT	denotes	the
T3902	16771-16776	JJ	denotes	human
T3903	16777-16781	NN	denotes	MCAD
T3901	16782-16788	NN	denotes	enzyme
T3904	16788-16789	.	denotes	.
T3905	16789-17031	sentence	denotes	This finding is similar to that seen with very long-chain acyl-CoA dehydrogenase (VLCAD) where mouse VLCAD is most active toward C16 acyl-substrates as compared to human VLCAD with the most enzymatic activity toward C14 acyl-substrates [16].
T3906	16790-16794	DT	denotes	This
T3907	16795-16802	NN	denotes	finding
T3908	16803-16805	VBZ	denotes	is
T3909	16806-16813	JJ	denotes	similar
T3910	16814-16816	IN	denotes	to
T3911	16817-16821	DT	denotes	that
T3912	16822-16826	VBN	denotes	seen
T3913	16827-16831	IN	denotes	with
T3914	16832-16836	RB	denotes	very
T3915	16837-16841	JJ	denotes	long
T3917	16841-16842	HYPH	denotes	-
T3916	16842-16847	NN	denotes	chain
T3919	16848-16852	NN	denotes	acyl
T3921	16852-16853	HYPH	denotes	-
T3920	16853-16856	NN	denotes	CoA
T3918	16857-16870	NN	denotes	dehydrogenase
T3922	16871-16872	-LRB-	denotes	(
T3923	16872-16877	NN	denotes	VLCAD
T3924	16877-16878	-RRB-	denotes	)
T3925	16879-16884	WRB	denotes	where
T3927	16885-16890	NN	denotes	mouse
T3928	16891-16896	NN	denotes	VLCAD
T3926	16897-16899	VBZ	denotes	is
T3929	16900-16904	RBS	denotes	most
T3930	16905-16911	JJ	denotes	active
T3931	16912-16918	IN	denotes	toward
T3932	16919-16922	NN	denotes	C16
T3934	16923-16927	NN	denotes	acyl
T3935	16927-16928	HYPH	denotes	-
T3933	16928-16938	NNS	denotes	substrates
T3936	16939-16941	IN	denotes	as
T3937	16942-16950	VBN	denotes	compared
T3938	16951-16953	IN	denotes	to
T3939	16954-16959	JJ	denotes	human
T3940	16960-16965	NN	denotes	VLCAD
T3941	16966-16970	IN	denotes	with
T3942	16971-16974	DT	denotes	the
T3944	16975-16979	RBS	denotes	most
T3945	16980-16989	JJ	denotes	enzymatic
T3943	16990-16998	NN	denotes	activity
T3946	16999-17005	IN	denotes	toward
T3947	17006-17009	NN	denotes	C14
T3949	17010-17014	NN	denotes	acyl
T3950	17014-17015	HYPH	denotes	-
T3948	17015-17025	NNS	denotes	substrates
T3951	17026-17027	-LRB-	denotes	[
T3952	17027-17029	CD	denotes	16
T3953	17029-17030	-RRB-	denotes	]
T3954	17030-17031	.	denotes	.
T3955	17031-17145	sentence	denotes	ETF reduction assays of liver homogenates were performed to characterize the MCAD−/− mice at the enzymatic level.
T3956	17032-17035	NN	denotes	ETF
T3958	17036-17045	NN	denotes	reduction
T3957	17046-17052	NNS	denotes	assays
T3960	17053-17055	IN	denotes	of
T3961	17056-17061	NN	denotes	liver
T3962	17062-17073	NNS	denotes	homogenates
T3963	17074-17078	VBD	denotes	were
T3959	17079-17088	VBN	denotes	performed
T3964	17089-17091	TO	denotes	to
T3965	17092-17104	VB	denotes	characterize
T3966	17105-17108	DT	denotes	the
T3968	17109-17113	NN	denotes	MCAD
T3969	17113-17114	SYM	denotes	−
T3970	17114-17115	HYPH	denotes	/
T3971	17115-17116	SYM	denotes	−
T3967	17117-17121	NNS	denotes	mice
T3972	17122-17124	IN	denotes	at
T3973	17125-17128	DT	denotes	the
T3975	17129-17138	JJ	denotes	enzymatic
T3974	17139-17144	NN	denotes	level
T3976	17144-17145	.	denotes	.
T3977	17145-17310	sentence	denotes	MCAD−/− mice had a significantly reduced ability to dehydrogenate C8-CoA, as is the case in human patients where MCAD activity is reduced to near zero with C8-CoA .
T3978	17146-17150	NN	denotes	MCAD
T3980	17150-17151	SYM	denotes	−
T3981	17151-17152	HYPH	denotes	/
T3982	17152-17153	SYM	denotes	−
T3979	17154-17158	NNS	denotes	mice
T3983	17159-17162	VBD	denotes	had
T3984	17163-17164	DT	denotes	a
T3986	17165-17178	RB	denotes	significantly
T3987	17179-17186	VBN	denotes	reduced
T3985	17187-17194	NN	denotes	ability
T3988	17195-17197	TO	denotes	to
T3989	17198-17211	VB	denotes	dehydrogenate
T3990	17212-17214	NN	denotes	C8
T3992	17214-17215	HYPH	denotes	-
T3991	17215-17218	NN	denotes	CoA
T3993	17218-17220	,	denotes	,
T3994	17220-17222	IN	denotes	as
T3995	17223-17225	VBZ	denotes	is
T3996	17226-17229	DT	denotes	the
T3997	17230-17234	NN	denotes	case
T3998	17235-17237	IN	denotes	in
T3999	17238-17243	JJ	denotes	human
T4000	17244-17252	NNS	denotes	patients
T4001	17253-17258	WRB	denotes	where
T4003	17259-17263	NN	denotes	MCAD
T4004	17264-17272	NN	denotes	activity
T4005	17273-17275	VBZ	denotes	is
T4002	17276-17283	VBN	denotes	reduced
T4006	17284-17286	IN	denotes	to
T4007	17287-17291	IN	denotes	near
T4008	17292-17296	CD	denotes	zero
T4009	17297-17301	IN	denotes	with
T4010	17302-17304	NN	denotes	C8
T4012	17304-17305	HYPH	denotes	-
T4011	17305-17308	NN	denotes	CoA
T4013	17309-17310	.	denotes	.
T4014	17310-17355	sentence	denotes	This was less so with palmitoyl-CoA (C16:0).
T4015	17311-17315	DT	denotes	This
T4016	17316-17319	VBD	denotes	was
T4017	17320-17324	RBR	denotes	less
T4018	17325-17327	RB	denotes	so
T4019	17328-17332	IN	denotes	with
T4020	17333-17342	NN	denotes	palmitoyl
T4022	17342-17343	HYPH	denotes	-
T4021	17343-17346	NN	denotes	CoA
T4023	17347-17348	-LRB-	denotes	(
T4024	17348-17351	NN	denotes	C16
T4025	17351-17352	:	denotes	:
T4026	17352-17353	CD	denotes	0
T4027	17353-17354	-RRB-	denotes	)
T4028	17354-17355	.	denotes	.
T4029	17355-17578	sentence	denotes	Because there was clearly no MCAD antigen detected in MCAD−/− mice, the residual dehydrogenase activity measured with these two substrates must represent the activity of other chain length–specific acyl-CoA dehydrogenases.
T4030	17356-17363	IN	denotes	Because
T4032	17364-17369	EX	denotes	there
T4031	17370-17373	VBD	denotes	was
T4034	17374-17381	RB	denotes	clearly
T4035	17382-17384	DT	denotes	no
T4037	17385-17389	NN	denotes	MCAD
T4036	17390-17397	NN	denotes	antigen
T4038	17398-17406	VBN	denotes	detected
T4039	17407-17409	IN	denotes	in
T4040	17410-17414	NN	denotes	MCAD
T4042	17414-17415	SYM	denotes	−
T4043	17415-17416	HYPH	denotes	/
T4044	17416-17417	SYM	denotes	−
T4041	17418-17422	NNS	denotes	mice
T4045	17422-17424	,	denotes	,
T4046	17424-17427	DT	denotes	the
T4048	17428-17436	JJ	denotes	residual
T4049	17437-17450	NN	denotes	dehydrogenase
T4047	17451-17459	NN	denotes	activity
T4050	17460-17468	VBN	denotes	measured
T4051	17469-17473	IN	denotes	with
T4052	17474-17479	DT	denotes	these
T4054	17480-17483	CD	denotes	two
T4053	17484-17494	NNS	denotes	substrates
T4055	17495-17499	MD	denotes	must
T4033	17500-17509	VB	denotes	represent
T4056	17510-17513	DT	denotes	the
T4057	17514-17522	NN	denotes	activity
T4058	17523-17525	IN	denotes	of
T4059	17526-17531	JJ	denotes	other
T4061	17532-17537	NN	denotes	chain
T4062	17538-17544	NN	denotes	length
T4064	17544-17545	HYPH	denotes	–
T4063	17545-17553	JJ	denotes	specific
T4065	17554-17558	NN	denotes	acyl
T4067	17558-17559	HYPH	denotes	-
T4066	17559-17562	NN	denotes	CoA
T4060	17563-17577	NNS	denotes	dehydrogenases
T4068	17577-17578	.	denotes	.
T4069	17578-17764	sentence	denotes	A high degree of neonatal mortality in MCAD−/− mice was a striking observation and appears to be analogous to the patterns of clinical episodes and mortality in MCAD-deficient patients.
T4070	17579-17580	DT	denotes	A
T4072	17581-17585	JJ	denotes	high
T4071	17586-17592	NN	denotes	degree
T4074	17593-17595	IN	denotes	of
T4075	17596-17604	JJ	denotes	neonatal
T4076	17605-17614	NN	denotes	mortality
T4077	17615-17617	IN	denotes	in
T4078	17618-17622	NN	denotes	MCAD
T4080	17622-17623	SYM	denotes	−
T4081	17623-17624	HYPH	denotes	/
T4082	17624-17625	SYM	denotes	−
T4079	17626-17630	NNS	denotes	mice
T4073	17631-17634	VBD	denotes	was
T4083	17635-17636	DT	denotes	a
T4085	17637-17645	JJ	denotes	striking
T4084	17646-17657	NN	denotes	observation
T4086	17658-17661	CC	denotes	and
T4087	17662-17669	VBZ	denotes	appears
T4088	17670-17672	TO	denotes	to
T4089	17673-17675	VB	denotes	be
T4090	17676-17685	JJ	denotes	analogous
T4091	17686-17688	IN	denotes	to
T4092	17689-17692	DT	denotes	the
T4093	17693-17701	NNS	denotes	patterns
T4094	17702-17704	IN	denotes	of
T4095	17705-17713	JJ	denotes	clinical
T4096	17714-17722	NNS	denotes	episodes
T4097	17723-17726	CC	denotes	and
T4098	17727-17736	NN	denotes	mortality
T4099	17737-17739	IN	denotes	in
T4100	17740-17744	NN	denotes	MCAD
T4102	17744-17745	HYPH	denotes	-
T4101	17745-17754	JJ	denotes	deficient
T4103	17755-17763	NNS	denotes	patients
T4104	17763-17764	.	denotes	.
T4105	17764-17900	sentence	denotes	MCAD−/− mice exhibited significant neonatal mortality with approximately 60% of the MCAD−/− pups dying prior to weaning at 3 wk of age.
T4106	17765-17769	NN	denotes	MCAD
T4108	17769-17770	SYM	denotes	−
T4109	17770-17771	HYPH	denotes	/
T4110	17771-17772	SYM	denotes	−
T4107	17773-17777	NNS	denotes	mice
T4111	17778-17787	VBD	denotes	exhibited
T4112	17788-17799	JJ	denotes	significant
T4114	17800-17808	JJ	denotes	neonatal
T4113	17809-17818	NN	denotes	mortality
T4115	17819-17823	IN	denotes	with
T4116	17824-17837	RB	denotes	approximately
T4117	17838-17840	CD	denotes	60
T4118	17840-17841	NN	denotes	%
T4120	17842-17844	IN	denotes	of
T4121	17845-17848	DT	denotes	the
T4123	17849-17853	NN	denotes	MCAD
T4124	17853-17854	SYM	denotes	−
T4125	17854-17855	HYPH	denotes	/
T4126	17855-17856	SYM	denotes	−
T4122	17857-17861	NNS	denotes	pups
T4119	17862-17867	VBG	denotes	dying
T4127	17868-17873	JJ	denotes	prior
T4128	17874-17876	IN	denotes	to
T4129	17877-17884	NN	denotes	weaning
T4130	17885-17887	IN	denotes	at
T4131	17888-17889	CD	denotes	3
T4132	17890-17892	NN	denotes	wk
T4133	17893-17895	IN	denotes	of
T4134	17896-17899	NN	denotes	age
T4135	17899-17900	.	denotes	.
T4136	17900-18066	sentence	denotes	Human patients present with hypoglycemia, hypoketonemia, and nonketotic organic aciduria precipitated by fasting, most frequently during the first 24 mo in life [1].
T4137	17901-17906	JJ	denotes	Human
T4138	17907-17915	NNS	denotes	patients
T4139	17916-17923	VBP	denotes	present
T4140	17924-17928	IN	denotes	with
T4141	17929-17941	NN	denotes	hypoglycemia
T4142	17941-17943	,	denotes	,
T4143	17943-17956	NN	denotes	hypoketonemia
T4144	17956-17958	,	denotes	,
T4145	17958-17961	CC	denotes	and
T4146	17962-17972	JJ	denotes	nonketotic
T4148	17973-17980	JJ	denotes	organic
T4147	17981-17989	NN	denotes	aciduria
T4149	17990-18002	VBN	denotes	precipitated
T4150	18003-18005	IN	denotes	by
T4151	18006-18013	NN	denotes	fasting
T4152	18013-18015	,	denotes	,
T4153	18015-18019	RBS	denotes	most
T4154	18020-18030	RB	denotes	frequently
T4155	18031-18037	IN	denotes	during
T4156	18038-18041	DT	denotes	the
T4158	18042-18047	JJ	denotes	first
T4159	18048-18050	CD	denotes	24
T4157	18051-18053	NN	denotes	mo
T4160	18054-18056	IN	denotes	in
T4161	18057-18061	NN	denotes	life
T4162	18062-18063	-LRB-	denotes	[
T4163	18063-18064	CD	denotes	1
T4164	18064-18065	-RRB-	denotes	]
T4165	18065-18066	.	denotes	.
T4166	18066-18225	sentence	denotes	It is likely that neonatal MCAD−/− pups are manifesting sensitivity to fasting with decompensation in a short period of time if maternal milk is not ingested.
T4167	18067-18069	PRP	denotes	It
T4168	18070-18072	VBZ	denotes	is
T4169	18073-18079	JJ	denotes	likely
T4170	18080-18084	IN	denotes	that
T4172	18085-18093	JJ	denotes	neonatal
T4174	18094-18098	NN	denotes	MCAD
T4175	18098-18099	SYM	denotes	−
T4176	18099-18100	HYPH	denotes	/
T4177	18100-18101	SYM	denotes	−
T4173	18102-18106	NNS	denotes	pups
T4178	18107-18110	VBP	denotes	are
T4171	18111-18122	VBG	denotes	manifesting
T4179	18123-18134	NN	denotes	sensitivity
T4180	18135-18137	IN	denotes	to
T4181	18138-18145	NN	denotes	fasting
T4182	18146-18150	IN	denotes	with
T4183	18151-18165	NN	denotes	decompensation
T4184	18166-18168	IN	denotes	in
T4185	18169-18170	DT	denotes	a
T4187	18171-18176	JJ	denotes	short
T4186	18177-18183	NN	denotes	period
T4188	18184-18186	IN	denotes	of
T4189	18187-18191	NN	denotes	time
T4190	18192-18194	IN	denotes	if
T4192	18195-18203	JJ	denotes	maternal
T4193	18204-18208	NN	denotes	milk
T4194	18209-18211	VBZ	denotes	is
T4195	18212-18215	RB	denotes	not
T4191	18216-18224	VBN	denotes	ingested
T4196	18224-18225	.	denotes	.
T4197	18225-18331	sentence	denotes	In contrast, no mortality was noted in adult MCAD−/− mice unless challenged with cold stress and fasting.
T4198	18226-18228	IN	denotes	In
T4200	18229-18237	NN	denotes	contrast
T4201	18237-18239	,	denotes	,
T4202	18239-18241	DT	denotes	no
T4203	18242-18251	NN	denotes	mortality
T4204	18252-18255	VBD	denotes	was
T4199	18256-18261	VBN	denotes	noted
T4205	18262-18264	IN	denotes	in
T4206	18265-18270	JJ	denotes	adult
T4208	18271-18275	NN	denotes	MCAD
T4209	18275-18276	SYM	denotes	−
T4210	18276-18277	HYPH	denotes	/
T4211	18277-18278	SYM	denotes	−
T4207	18279-18283	NNS	denotes	mice
T4212	18284-18290	IN	denotes	unless
T4213	18291-18301	VBN	denotes	challenged
T4214	18302-18306	IN	denotes	with
T4215	18307-18311	NN	denotes	cold
T4216	18312-18318	NN	denotes	stress
T4217	18319-18322	CC	denotes	and
T4218	18323-18330	NN	denotes	fasting
T4219	18330-18331	.	denotes	.
T4220	18331-18428	sentence	denotes	Under cold challenge conditions, however, MCAD−/− mice were unable to maintain body temperature.
T4221	18332-18337	IN	denotes	Under
T4223	18338-18342	NN	denotes	cold
T4224	18343-18352	NN	denotes	challenge
T4225	18353-18363	NNS	denotes	conditions
T4226	18363-18365	,	denotes	,
T4227	18365-18372	RB	denotes	however
T4228	18372-18374	,	denotes	,
T4229	18374-18378	NN	denotes	MCAD
T4231	18378-18379	SYM	denotes	−
T4232	18379-18380	HYPH	denotes	/
T4233	18380-18381	SYM	denotes	−
T4230	18382-18386	NNS	denotes	mice
T4222	18387-18391	VBD	denotes	were
T4234	18392-18398	JJ	denotes	unable
T4235	18399-18401	TO	denotes	to
T4236	18402-18410	VB	denotes	maintain
T4237	18411-18415	NN	denotes	body
T4238	18416-18427	NN	denotes	temperature
T4239	18427-18428	.	denotes	.
T4240	18428-18535	sentence	denotes	Brown fat is predominantly responsible for thermogenesis and normally expresses high levels of Acadm mRNA.
T4241	18429-18434	JJ	denotes	Brown
T4242	18435-18438	NN	denotes	fat
T4243	18439-18441	VBZ	denotes	is
T4244	18442-18455	RB	denotes	predominantly
T4245	18456-18467	JJ	denotes	responsible
T4246	18468-18471	IN	denotes	for
T4247	18472-18485	NN	denotes	thermogenesis
T4248	18486-18489	CC	denotes	and
T4249	18490-18498	RB	denotes	normally
T4250	18499-18508	VBZ	denotes	expresses
T4251	18509-18513	JJ	denotes	high
T4252	18514-18520	NNS	denotes	levels
T4253	18521-18523	IN	denotes	of
T4254	18524-18529	NN	denotes	Acadm
T4255	18530-18534	NN	denotes	mRNA
T4256	18534-18535	.	denotes	.
T4257	18535-18720	sentence	denotes	The microvesicular and macrovesicular hepatic steatosis seen in fasted MCAD−/− mice is consistent with the primary pathological finding seen in human MCAD patients with fasting stress.
T4258	18536-18539	DT	denotes	The
T4260	18540-18554	JJ	denotes	microvesicular
T4261	18555-18558	CC	denotes	and
T4262	18559-18573	JJ	denotes	macrovesicular
T4263	18574-18581	JJ	denotes	hepatic
T4259	18582-18591	NN	denotes	steatosis
T4265	18592-18596	VBN	denotes	seen
T4266	18597-18599	IN	denotes	in
T4267	18600-18606	VBN	denotes	fasted
T4269	18607-18611	NN	denotes	MCAD
T4270	18611-18612	SYM	denotes	−
T4271	18612-18613	HYPH	denotes	/
T4272	18613-18614	SYM	denotes	−
T4268	18615-18619	NNS	denotes	mice
T4264	18620-18622	VBZ	denotes	is
T4273	18623-18633	JJ	denotes	consistent
T4274	18634-18638	IN	denotes	with
T4275	18639-18642	DT	denotes	the
T4277	18643-18650	JJ	denotes	primary
T4278	18651-18663	JJ	denotes	pathological
T4276	18664-18671	NN	denotes	finding
T4279	18672-18676	VBN	denotes	seen
T4280	18677-18679	IN	denotes	in
T4281	18680-18685	JJ	denotes	human
T4283	18686-18690	NN	denotes	MCAD
T4282	18691-18699	NNS	denotes	patients
T4284	18700-18704	IN	denotes	with
T4285	18705-18712	NN	denotes	fasting
T4286	18713-18719	NN	denotes	stress
T4287	18719-18720	.	denotes	.
T4288	18720-18815	sentence	denotes	Sporadic cardiac lesions in MCAD−/− mice, however, were an interesting and unexpected finding.
T4289	18721-18729	JJ	denotes	Sporadic
T4291	18730-18737	JJ	denotes	cardiac
T4290	18738-18745	NNS	denotes	lesions
T4293	18746-18748	IN	denotes	in
T4294	18749-18753	NN	denotes	MCAD
T4296	18753-18754	SYM	denotes	−
T4297	18754-18755	HYPH	denotes	/
T4298	18755-18756	SYM	denotes	−
T4295	18757-18761	NNS	denotes	mice
T4299	18761-18763	,	denotes	,
T4300	18763-18770	RB	denotes	however
T4301	18770-18772	,	denotes	,
T4292	18772-18776	VBD	denotes	were
T4302	18777-18779	DT	denotes	an
T4304	18780-18791	JJ	denotes	interesting
T4305	18792-18795	CC	denotes	and
T4306	18796-18806	JJ	denotes	unexpected
T4303	18807-18814	NN	denotes	finding
T4307	18814-18815	.	denotes	.
T4308	18815-19064	sentence	denotes	The diffuse cardiomyopathy with multifocal myocyte degeneration and necrosis observed in MCAD−/− mice has not been reported in human MCAD patients, however, cardiac arrhythmias and dysfunction have been reported in MCAD-deficient patients [17, 18].
T4309	18816-18819	DT	denotes	The
T4311	18820-18827	JJ	denotes	diffuse
T4310	18828-18842	NN	denotes	cardiomyopathy
T4313	18843-18847	IN	denotes	with
T4314	18848-18858	JJ	denotes	multifocal
T4316	18859-18866	NN	denotes	myocyte
T4315	18867-18879	NN	denotes	degeneration
T4317	18880-18883	CC	denotes	and
T4318	18884-18892	NN	denotes	necrosis
T4319	18893-18901	VBN	denotes	observed
T4320	18902-18904	IN	denotes	in
T4321	18905-18909	NN	denotes	MCAD
T4323	18909-18910	SYM	denotes	−
T4324	18910-18911	HYPH	denotes	/
T4325	18911-18912	SYM	denotes	−
T4322	18913-18917	NNS	denotes	mice
T4326	18918-18921	VBZ	denotes	has
T4327	18922-18925	RB	denotes	not
T4328	18926-18930	VBN	denotes	been
T4312	18931-18939	VBN	denotes	reported
T4330	18940-18942	IN	denotes	in
T4331	18943-18948	JJ	denotes	human
T4333	18949-18953	NN	denotes	MCAD
T4332	18954-18962	NNS	denotes	patients
T4334	18962-18964	,	denotes	,
T4335	18964-18971	RB	denotes	however
T4336	18971-18973	,	denotes	,
T4337	18973-18980	JJ	denotes	cardiac
T4338	18981-18992	NNS	denotes	arrhythmias
T4339	18993-18996	CC	denotes	and
T4340	18997-19008	NN	denotes	dysfunction
T4341	19009-19013	VBP	denotes	have
T4342	19014-19018	VBN	denotes	been
T4329	19019-19027	VBN	denotes	reported
T4343	19028-19030	IN	denotes	in
T4344	19031-19035	NN	denotes	MCAD
T4346	19035-19036	HYPH	denotes	-
T4345	19036-19045	JJ	denotes	deficient
T4347	19046-19054	NNS	denotes	patients
T4348	19055-19056	-LRB-	denotes	[
T4350	19056-19058	CD	denotes	17
T4351	19058-19060	,	denotes	,
T4349	19060-19062	CD	denotes	18
T4352	19062-19063	-RRB-	denotes	]
T4353	19063-19064	.	denotes	.
T4354	19064-19232	sentence	denotes	Interestingly, cardiomyopathy has been observed in VLCAD deficiency [19] and other disorders of long chain fat metabolism such as severe CPT-1 and -2 deficiencies [1].
T4355	19065-19078	RB	denotes	Interestingly
T4357	19078-19080	,	denotes	,
T4358	19080-19094	NN	denotes	cardiomyopathy
T4359	19095-19098	VBZ	denotes	has
T4360	19099-19103	VBN	denotes	been
T4356	19104-19112	VBN	denotes	observed
T4361	19113-19115	IN	denotes	in
T4362	19116-19121	NN	denotes	VLCAD
T4363	19122-19132	NN	denotes	deficiency
T4364	19133-19134	-LRB-	denotes	[
T4365	19134-19136	CD	denotes	19
T4366	19136-19137	-RRB-	denotes	]
T4367	19138-19141	CC	denotes	and
T4368	19142-19147	JJ	denotes	other
T4369	19148-19157	NNS	denotes	disorders
T4370	19158-19160	IN	denotes	of
T4371	19161-19165	JJ	denotes	long
T4372	19166-19171	NN	denotes	chain
T4373	19172-19175	NN	denotes	fat
T4374	19176-19186	NN	denotes	metabolism
T4375	19187-19191	JJ	denotes	such
T4376	19192-19194	IN	denotes	as
T4377	19195-19201	JJ	denotes	severe
T4379	19202-19205	NN	denotes	CPT
T4381	19205-19206	HYPH	denotes	-
T4380	19206-19207	CD	denotes	1
T4382	19208-19211	CC	denotes	and
T4383	19212-19213	HYPH	denotes	-
T4384	19213-19214	CD	denotes	2
T4378	19215-19227	NNS	denotes	deficiencies
T4385	19228-19229	-LRB-	denotes	[
T4386	19229-19230	CD	denotes	1
T4387	19230-19231	-RRB-	denotes	]
T4388	19231-19232	.	denotes	.
T4389	19232-19366	sentence	denotes	Thus it is tempting to relate the cardiac problems in the mouse to the apparent broader range of substrate utilization of mouse MCAD.
T4390	19233-19237	RB	denotes	Thus
T4392	19238-19240	PRP	denotes	it
T4391	19241-19243	VBZ	denotes	is
T4393	19244-19252	JJ	denotes	tempting
T4394	19253-19255	TO	denotes	to
T4395	19256-19262	VB	denotes	relate
T4396	19263-19266	DT	denotes	the
T4398	19267-19274	JJ	denotes	cardiac
T4397	19275-19283	NNS	denotes	problems
T4399	19284-19286	IN	denotes	in
T4400	19287-19290	DT	denotes	the
T4401	19291-19296	NN	denotes	mouse
T4402	19297-19299	IN	denotes	to
T4403	19300-19303	DT	denotes	the
T4405	19304-19312	JJ	denotes	apparent
T4406	19313-19320	JJR	denotes	broader
T4404	19321-19326	NN	denotes	range
T4407	19327-19329	IN	denotes	of
T4408	19330-19339	NN	denotes	substrate
T4409	19340-19351	NN	denotes	utilization
T4410	19352-19354	IN	denotes	of
T4411	19355-19360	NN	denotes	mouse
T4412	19361-19365	NN	denotes	MCAD
T4413	19365-19366	.	denotes	.
T4414	19366-19549	sentence	denotes	The inconsistent liver and cardiac lesions in these mice is analogous with the significant inter- and intrafamilial phenotypic heterogeneity seen in MCAD deficiency in humans [1,20].
T4415	19367-19370	DT	denotes	The
T4417	19371-19383	JJ	denotes	inconsistent
T4418	19384-19389	NN	denotes	liver
T4419	19390-19393	CC	denotes	and
T4420	19394-19401	JJ	denotes	cardiac
T4416	19402-19409	NNS	denotes	lesions
T4422	19410-19412	IN	denotes	in
T4423	19413-19418	DT	denotes	these
T4424	19419-19423	NNS	denotes	mice
T4421	19424-19426	VBZ	denotes	is
T4425	19427-19436	JJ	denotes	analogous
T4426	19437-19441	IN	denotes	with
T4427	19442-19445	DT	denotes	the
T4429	19446-19457	JJ	denotes	significant
T4430	19458-19463	AFX	denotes	inter
T4432	19463-19464	HYPH	denotes	-
T4433	19465-19468	CC	denotes	and
T4431	19469-19482	JJ	denotes	intrafamilial
T4434	19483-19493	JJ	denotes	phenotypic
T4428	19494-19507	NN	denotes	heterogeneity
T4435	19508-19512	VBN	denotes	seen
T4436	19513-19515	IN	denotes	in
T4437	19516-19520	NN	denotes	MCAD
T4438	19521-19531	NN	denotes	deficiency
T4439	19532-19534	IN	denotes	in
T4440	19535-19541	NNS	denotes	humans
T4441	19542-19543	-LRB-	denotes	[
T4443	19543-19544	CD	denotes	1
T4444	19544-19545	,	denotes	,
T4442	19545-19547	CD	denotes	20
T4445	19547-19548	-RRB-	denotes	]
T4446	19548-19549	.	denotes	.
T4447	19549-19814	sentence	denotes	In comparisons with our experiences with the other mouse models for acyl-CoA dehydrogenase deficiencies, the overall phenotype of MCAD−/− mice is less severe than that found in LCAD−/− mice, yet more pronounced than the VLCAD−/− or SCAD−/− mouse models [16,21,22].
T4448	19550-19552	IN	denotes	In
T4450	19553-19564	NNS	denotes	comparisons
T4451	19565-19569	IN	denotes	with
T4452	19570-19573	PRP$	denotes	our
T4453	19574-19585	NNS	denotes	experiences
T4454	19586-19590	IN	denotes	with
T4455	19591-19594	DT	denotes	the
T4457	19595-19600	JJ	denotes	other
T4458	19601-19606	NN	denotes	mouse
T4456	19607-19613	NNS	denotes	models
T4459	19614-19617	IN	denotes	for
T4460	19618-19622	NN	denotes	acyl
T4462	19622-19623	HYPH	denotes	-
T4461	19623-19626	NN	denotes	CoA
T4463	19627-19640	NN	denotes	dehydrogenase
T4464	19641-19653	NNS	denotes	deficiencies
T4465	19653-19655	,	denotes	,
T4466	19655-19658	DT	denotes	the
T4468	19659-19666	JJ	denotes	overall
T4467	19667-19676	NN	denotes	phenotype
T4469	19677-19679	IN	denotes	of
T4470	19680-19684	NN	denotes	MCAD
T4472	19684-19685	SYM	denotes	−
T4473	19685-19686	HYPH	denotes	/
T4474	19686-19687	SYM	denotes	−
T4471	19688-19692	NNS	denotes	mice
T4449	19693-19695	VBZ	denotes	is
T4475	19696-19700	RBR	denotes	less
T4476	19701-19707	JJ	denotes	severe
T4477	19708-19712	IN	denotes	than
T4478	19713-19717	DT	denotes	that
T4479	19718-19723	VBN	denotes	found
T4480	19724-19726	IN	denotes	in
T4481	19727-19731	NN	denotes	LCAD
T4483	19731-19732	SYM	denotes	−
T4484	19732-19733	HYPH	denotes	/
T4485	19733-19734	SYM	denotes	−
T4482	19735-19739	NNS	denotes	mice
T4486	19739-19741	,	denotes	,
T4487	19741-19744	CC	denotes	yet
T4488	19745-19749	RBR	denotes	more
T4489	19750-19760	JJ	denotes	pronounced
T4490	19761-19765	IN	denotes	than
T4491	19766-19769	DT	denotes	the
T4493	19770-19775	NN	denotes	VLCAD
T4494	19775-19776	SYM	denotes	−
T4495	19776-19777	HYPH	denotes	/
T4496	19777-19778	SYM	denotes	−
T4497	19779-19781	CC	denotes	or
T4498	19782-19786	NN	denotes	SCAD
T4499	19786-19787	SYM	denotes	−
T4500	19787-19788	HYPH	denotes	/
T4501	19788-19789	SYM	denotes	−
T4502	19790-19795	NN	denotes	mouse
T4492	19796-19802	NNS	denotes	models
T4503	19803-19804	-LRB-	denotes	[
T4505	19804-19806	CD	denotes	16
T4506	19806-19807	,	denotes	,
T4507	19807-19809	CD	denotes	21
T4508	19809-19810	,	denotes	,
T4504	19810-19812	CD	denotes	22
T4509	19812-19813	-RRB-	denotes	]
T4510	19813-19814	.	denotes	.
T4511	19814-19929	sentence	denotes	All of these mutants are cold intolerant and display varying degrees of fatty changes in liver, heart, and kidney.
T4512	19815-19818	DT	denotes	All
T4514	19819-19821	IN	denotes	of
T4515	19822-19827	DT	denotes	these
T4516	19828-19835	NNS	denotes	mutants
T4513	19836-19839	VBP	denotes	are
T4517	19840-19844	NN	denotes	cold
T4518	19845-19855	JJ	denotes	intolerant
T4519	19856-19859	CC	denotes	and
T4520	19860-19867	VBP	denotes	display
T4521	19868-19875	VBG	denotes	varying
T4522	19876-19883	NNS	denotes	degrees
T4523	19884-19886	IN	denotes	of
T4524	19887-19892	JJ	denotes	fatty
T4525	19893-19900	NNS	denotes	changes
T4526	19901-19903	IN	denotes	in
T4527	19904-19909	NN	denotes	liver
T4528	19909-19911	,	denotes	,
T4529	19911-19916	NN	denotes	heart
T4530	19916-19918	,	denotes	,
T4531	19918-19921	CC	denotes	and
T4532	19922-19928	NN	denotes	kidney
T4533	19928-19929	.	denotes	.
T4534	19929-20028	sentence	denotes	LCAD−/− mice show more spontaneous deaths and gestational losses than the other deficiencies [21].
T4535	19930-19934	NN	denotes	LCAD
T4537	19934-19935	SYM	denotes	−
T4538	19935-19936	HYPH	denotes	/
T4539	19936-19937	SYM	denotes	−
T4536	19938-19942	NNS	denotes	mice
T4540	19943-19947	VBP	denotes	show
T4541	19948-19952	JJR	denotes	more
T4543	19953-19964	JJ	denotes	spontaneous
T4542	19965-19971	NNS	denotes	deaths
T4544	19972-19975	CC	denotes	and
T4545	19976-19987	JJ	denotes	gestational
T4546	19988-19994	NNS	denotes	losses
T4547	19995-19999	IN	denotes	than
T4548	20000-20003	DT	denotes	the
T4550	20004-20009	JJ	denotes	other
T4549	20010-20022	NNS	denotes	deficiencies
T4551	20023-20024	-LRB-	denotes	[
T4552	20024-20026	CD	denotes	21
T4553	20026-20027	-RRB-	denotes	]
T4554	20027-20028	.	denotes	.
T4555	20028-20191	sentence	denotes	The significant neonatal mortality in MCAD−/− mice is distinctive from these other mouse models suggesting a greater degree of sensitivity to fasting intolerance.
T4556	20029-20032	DT	denotes	The
T4558	20033-20044	JJ	denotes	significant
T4559	20045-20053	JJ	denotes	neonatal
T4557	20054-20063	NN	denotes	mortality
T4561	20064-20066	IN	denotes	in
T4562	20067-20071	NN	denotes	MCAD
T4564	20071-20072	SYM	denotes	−
T4565	20072-20073	HYPH	denotes	/
T4566	20073-20074	SYM	denotes	−
T4563	20075-20079	NNS	denotes	mice
T4560	20080-20082	VBZ	denotes	is
T4567	20083-20094	JJ	denotes	distinctive
T4568	20095-20099	IN	denotes	from
T4569	20100-20105	DT	denotes	these
T4571	20106-20111	JJ	denotes	other
T4572	20112-20117	NN	denotes	mouse
T4570	20118-20124	NNS	denotes	models
T4573	20125-20135	VBG	denotes	suggesting
T4574	20136-20137	DT	denotes	a
T4576	20138-20145	JJR	denotes	greater
T4575	20146-20152	NN	denotes	degree
T4577	20153-20155	IN	denotes	of
T4578	20156-20167	NN	denotes	sensitivity
T4579	20168-20170	IN	denotes	to
T4580	20171-20178	NN	denotes	fasting
T4581	20179-20190	NN	denotes	intolerance
T4582	20190-20191	.	denotes	.
T4583	20191-20310	sentence	denotes	The phenotypes of both the VLCAD−/− and SCAD−/− mice are relatively mild if the animals are not cold stressed [16,22].
T4584	20192-20195	DT	denotes	The
T4585	20196-20206	NNS	denotes	phenotypes
T4587	20207-20209	IN	denotes	of
T4588	20210-20214	CC	denotes	both
T4590	20215-20218	DT	denotes	the
T4591	20219-20224	NN	denotes	VLCAD
T4592	20224-20225	SYM	denotes	−
T4593	20225-20226	HYPH	denotes	/
T4594	20226-20227	SYM	denotes	−
T4595	20228-20231	CC	denotes	and
T4596	20232-20236	NN	denotes	SCAD
T4597	20236-20237	SYM	denotes	−
T4598	20237-20238	HYPH	denotes	/
T4599	20238-20239	SYM	denotes	−
T4589	20240-20244	NNS	denotes	mice
T4586	20245-20248	VBP	denotes	are
T4600	20249-20259	RB	denotes	relatively
T4601	20260-20264	JJ	denotes	mild
T4602	20265-20267	IN	denotes	if
T4604	20268-20271	DT	denotes	the
T4605	20272-20279	NNS	denotes	animals
T4606	20280-20283	VBP	denotes	are
T4607	20284-20287	RB	denotes	not
T4608	20288-20292	JJ	denotes	cold
T4603	20293-20301	VBN	denotes	stressed
T4609	20302-20303	-LRB-	denotes	[
T4611	20303-20305	CD	denotes	16
T4612	20305-20306	,	denotes	,
T4610	20306-20308	CD	denotes	22
T4613	20308-20309	-RRB-	denotes	]
T4614	20309-20310	.	denotes	.
T4615	20310-20523	sentence	denotes	The MCAD-deficient mouse offers new insights into the pathogenesis of mitochondrial β-oxidation deficiencies and will provide a robust tool to better understand the role of fatty acids in other relevant diseases.
T4616	20311-20314	DT	denotes	The
T4618	20315-20319	JJ	denotes	MCAD
T4620	20319-20320	HYPH	denotes	-
T4619	20320-20329	JJ	denotes	deficient
T4617	20330-20335	NN	denotes	mouse
T4621	20336-20342	VBZ	denotes	offers
T4622	20343-20346	JJ	denotes	new
T4623	20347-20355	NNS	denotes	insights
T4624	20356-20360	IN	denotes	into
T4625	20361-20364	DT	denotes	the
T4626	20365-20377	NN	denotes	pathogenesis
T4627	20378-20380	IN	denotes	of
T4628	20381-20394	JJ	denotes	mitochondrial
T4630	20395-20396	NN	denotes	β
T4632	20396-20397	HYPH	denotes	-
T4631	20397-20406	NN	denotes	oxidation
T4629	20407-20419	NNS	denotes	deficiencies
T4633	20420-20423	CC	denotes	and
T4634	20424-20428	MD	denotes	will
T4635	20429-20436	VB	denotes	provide
T4636	20437-20438	DT	denotes	a
T4638	20439-20445	JJ	denotes	robust
T4637	20446-20450	NN	denotes	tool
T4639	20451-20453	TO	denotes	to
T4641	20454-20460	RBR	denotes	better
T4640	20461-20471	VB	denotes	understand
T4642	20472-20475	DT	denotes	the
T4643	20476-20480	NN	denotes	role
T4644	20481-20483	IN	denotes	of
T4645	20484-20489	JJ	denotes	fatty
T4646	20490-20495	NNS	denotes	acids
T4647	20496-20498	IN	denotes	in
T4648	20499-20504	JJ	denotes	other
T4650	20505-20513	JJ	denotes	relevant
T4649	20514-20522	NNS	denotes	diseases
T4651	20522-20523	.	denotes	.
T4937	20548-20560	NN	denotes	Construction
T4938	20561-20563	IN	denotes	of
T4939	20564-20568	NN	denotes	MCAD
T4941	20569-20578	NN	denotes	targeting
T4940	20579-20585	NN	denotes	vector
T4942	20585-20586	.	denotes	.
T4943	20586-20676	sentence	denotes	A neomycin resistance gene cassette [23] was subcloned into the SalI site of pGEM11Zf(+).
T4944	20587-20588	DT	denotes	A
T4946	20589-20597	NN	denotes	neomycin
T4947	20598-20608	NN	denotes	resistance
T4948	20609-20613	NN	denotes	gene
T4945	20614-20622	NN	denotes	cassette
T4950	20623-20624	-LRB-	denotes	[
T4951	20624-20626	CD	denotes	23
T4952	20626-20627	-RRB-	denotes	]
T4953	20628-20631	VBD	denotes	was
T4949	20632-20641	VBN	denotes	subcloned
T4954	20642-20646	IN	denotes	into
T4955	20647-20650	DT	denotes	the
T4957	20651-20655	NN	denotes	SalI
T4956	20656-20660	NN	denotes	site
T4958	20661-20663	IN	denotes	of
T4959	20664-20672	NN	denotes	pGEM11Zf
T4960	20672-20673	-LRB-	denotes	(
T4961	20673-20674	SYM	denotes	+
T4962	20674-20675	-RRB-	denotes	)
T4963	20675-20676	.	denotes	.
T4964	20676-20762	sentence	denotes	The plasmid was digested with EcoRI and the overhangs were filled with Klenow enzyme.
T4965	20677-20680	DT	denotes	The
T4966	20681-20688	NN	denotes	plasmid
T4968	20689-20692	VBD	denotes	was
T4967	20693-20701	VBN	denotes	digested
T4969	20702-20706	IN	denotes	with
T4970	20707-20712	NN	denotes	EcoRI
T4971	20713-20716	CC	denotes	and
T4972	20717-20720	DT	denotes	the
T4973	20721-20730	NNS	denotes	overhangs
T4975	20731-20735	VBD	denotes	were
T4974	20736-20742	VBN	denotes	filled
T4976	20743-20747	IN	denotes	with
T4977	20748-20754	NN	denotes	Klenow
T4978	20755-20761	NN	denotes	enzyme
T4979	20761-20762	.	denotes	.
T4980	20762-20905	sentence	denotes	Subsequent ligation of the blunt ends recircularized the vector and destroyed the EcoRI site within the polylinker of the pGEMl1Zf(+) plasmid.
T4981	20763-20773	JJ	denotes	Subsequent
T4982	20774-20782	NN	denotes	ligation
T4984	20783-20785	IN	denotes	of
T4985	20786-20789	DT	denotes	the
T4987	20790-20795	NN	denotes	blunt
T4986	20796-20800	NNS	denotes	ends
T4983	20801-20815	VBD	denotes	recircularized
T4988	20816-20819	DT	denotes	the
T4989	20820-20826	NN	denotes	vector
T4990	20827-20830	CC	denotes	and
T4991	20831-20840	VBD	denotes	destroyed
T4992	20841-20844	DT	denotes	the
T4994	20845-20850	NN	denotes	EcoRI
T4993	20851-20855	NN	denotes	site
T4995	20856-20862	IN	denotes	within
T4996	20863-20866	DT	denotes	the
T4997	20867-20877	NN	denotes	polylinker
T4998	20878-20880	IN	denotes	of
T4999	20881-20884	DT	denotes	the
T5001	20885-20893	NN	denotes	pGEMl1Zf
T5002	20893-20894	-LRB-	denotes	(
T5003	20894-20895	SYM	denotes	+
T5004	20895-20896	-RRB-	denotes	)
T5000	20897-20904	NN	denotes	plasmid
T5005	20904-20905	.	denotes	.
T5006	20905-21148	sentence	denotes	Next, an 8-kb Acadm genomic fragment spanning exons 8, 9, and 10 and flanking intron sequences, originally obtained from a Lambda FIXII 129Sv mouse genomic library [24,25], was directionally cloned into the NotI and XhoI sites of pGEM11Zf(+).
T5007	20906-20910	RB	denotes	Next
T5009	20910-20912	,	denotes	,
T5010	20912-20914	DT	denotes	an
T5012	20915-20916	CD	denotes	8
T5014	20916-20917	HYPH	denotes	-
T5013	20917-20919	NN	denotes	kb
T5015	20920-20925	NN	denotes	Acadm
T5016	20926-20933	JJ	denotes	genomic
T5011	20934-20942	NN	denotes	fragment
T5017	20943-20951	VBG	denotes	spanning
T5018	20952-20957	NNS	denotes	exons
T5019	20958-20959	CD	denotes	8
T5020	20959-20961	,	denotes	,
T5021	20961-20962	CD	denotes	9
T5022	20962-20964	,	denotes	,
T5023	20964-20967	CC	denotes	and
T5024	20968-20970	CD	denotes	10
T5025	20971-20974	CC	denotes	and
T5026	20975-20983	VBG	denotes	flanking
T5027	20984-20990	NN	denotes	intron
T5028	20991-21000	NNS	denotes	sequences
T5029	21000-21002	,	denotes	,
T5030	21002-21012	RB	denotes	originally
T5031	21013-21021	VBN	denotes	obtained
T5032	21022-21026	IN	denotes	from
T5033	21027-21028	DT	denotes	a
T5035	21029-21035	NNP	denotes	Lambda
T5036	21036-21041	NN	denotes	FIXII
T5037	21042-21047	NN	denotes	129Sv
T5038	21048-21053	NN	denotes	mouse
T5039	21054-21061	JJ	denotes	genomic
T5034	21062-21069	NN	denotes	library
T5040	21070-21071	-LRB-	denotes	[
T5042	21071-21073	CD	denotes	24
T5043	21073-21074	,	denotes	,
T5041	21074-21076	CD	denotes	25
T5044	21076-21077	-RRB-	denotes	]
T5045	21077-21079	,	denotes	,
T5046	21079-21082	VBD	denotes	was
T5047	21083-21096	RB	denotes	directionally
T5008	21097-21103	VBN	denotes	cloned
T5048	21104-21108	IN	denotes	into
T5049	21109-21112	DT	denotes	the
T5051	21113-21117	NN	denotes	NotI
T5052	21118-21121	CC	denotes	and
T5053	21122-21126	NN	denotes	XhoI
T5050	21127-21132	NNS	denotes	sites
T5054	21133-21135	IN	denotes	of
T5055	21136-21144	NN	denotes	pGEM11Zf
T5056	21144-21145	-LRB-	denotes	(
T5057	21145-21146	SYM	denotes	+
T5058	21146-21147	-RRB-	denotes	)
T5059	21147-21148	.	denotes	.
T5060	21148-21291	sentence	denotes	The vector was digested with BamHI and EcoRI to remove a 1.3-kb BamHI/EcoRI genomic fragment containing exon 10 and flanking intron sequences.
T5061	21149-21152	DT	denotes	The
T5062	21153-21159	NN	denotes	vector
T5064	21160-21163	VBD	denotes	was
T5063	21164-21172	VBN	denotes	digested
T5065	21173-21177	IN	denotes	with
T5066	21178-21183	NN	denotes	BamHI
T5067	21184-21187	CC	denotes	and
T5068	21188-21193	NN	denotes	EcoRI
T5069	21194-21196	TO	denotes	to
T5070	21197-21203	VB	denotes	remove
T5071	21204-21205	DT	denotes	a
T5073	21206-21209	CD	denotes	1.3
T5075	21209-21210	HYPH	denotes	-
T5074	21210-21212	NN	denotes	kb
T5076	21213-21218	NN	denotes	BamHI
T5078	21218-21219	HYPH	denotes	/
T5077	21219-21224	NN	denotes	EcoRI
T5079	21225-21232	JJ	denotes	genomic
T5072	21233-21241	NN	denotes	fragment
T5080	21242-21252	VBG	denotes	containing
T5081	21253-21257	NN	denotes	exon
T5082	21258-21260	CD	denotes	10
T5083	21261-21264	CC	denotes	and
T5084	21265-21273	VBG	denotes	flanking
T5085	21274-21280	NN	denotes	intron
T5086	21281-21290	NNS	denotes	sequences
T5087	21290-21291	.	denotes	.
T5088	21291-21515	sentence	denotes	The digested vector, without the 1.3-kb BamHI/EcoRI genomic fragment, was purified by gel purification and recircularized by ligation using three oligonucleotides: 5′-AATTGTCGACA-3′; 5′GATCGTCGACA-3′; and 5′-TCGATGTCGAC-3′.
T5089	21292-21295	DT	denotes	The
T5091	21296-21304	VBN	denotes	digested
T5090	21305-21311	NN	denotes	vector
T5093	21311-21313	,	denotes	,
T5094	21313-21320	IN	denotes	without
T5095	21321-21324	DT	denotes	the
T5097	21325-21328	CD	denotes	1.3
T5099	21328-21329	HYPH	denotes	-
T5098	21329-21331	NN	denotes	kb
T5100	21332-21337	NN	denotes	BamHI
T5102	21337-21338	HYPH	denotes	/
T5101	21338-21343	NN	denotes	EcoRI
T5103	21344-21351	JJ	denotes	genomic
T5096	21352-21360	NN	denotes	fragment
T5104	21360-21362	,	denotes	,
T5105	21362-21365	VBD	denotes	was
T5092	21366-21374	VBN	denotes	purified
T5106	21375-21377	IN	denotes	by
T5107	21378-21381	NN	denotes	gel
T5108	21382-21394	NN	denotes	purification
T5109	21395-21398	CC	denotes	and
T5110	21399-21413	VBN	denotes	recircularized
T5111	21414-21416	IN	denotes	by
T5112	21417-21425	NN	denotes	ligation
T5113	21426-21431	VBG	denotes	using
T5114	21432-21437	CD	denotes	three
T5115	21438-21454	NNS	denotes	oligonucleotides
T5116	21454-21456	:	denotes	:
T5117	21456-21457	CD	denotes	5
T5119	21457-21458	SYM	denotes	′
T5120	21458-21459	HYPH	denotes	-
T5118	21459-21470	NN	denotes	AATTGTCGACA
T5121	21470-21471	HYPH	denotes	-
T5122	21471-21472	CD	denotes	3
T5123	21472-21473	SYM	denotes	′
T5124	21473-21474	:	denotes	;
T5125	21475-21476	CD	denotes	5
T5127	21476-21477	SYM	denotes	′
T5126	21477-21488	NN	denotes	GATCGTCGACA
T5128	21488-21489	HYPH	denotes	-
T5129	21489-21490	CD	denotes	3
T5130	21490-21491	SYM	denotes	′
T5131	21491-21492	:	denotes	;
T5132	21493-21496	CC	denotes	and
T5133	21497-21498	CD	denotes	5
T5135	21498-21499	SYM	denotes	′
T5136	21499-21500	HYPH	denotes	-
T5134	21500-21511	NN	denotes	TCGATGTCGAC
T5137	21511-21512	HYPH	denotes	-
T5138	21512-21513	CD	denotes	3
T5139	21513-21514	SYM	denotes	′
T5140	21514-21515	.	denotes	.
T5141	21515-21690	sentence	denotes	The recircularized vector, resulting from the ligation of the long arm to the short arm of homology, contained a unique SalI site where the 1.3-kb exon 10 region was deleted.
T5142	21516-21519	DT	denotes	The
T5144	21520-21534	VBN	denotes	recircularized
T5143	21535-21541	NN	denotes	vector
T5146	21541-21543	,	denotes	,
T5147	21543-21552	VBG	denotes	resulting
T5148	21553-21557	IN	denotes	from
T5149	21558-21561	DT	denotes	the
T5150	21562-21570	NN	denotes	ligation
T5151	21571-21573	IN	denotes	of
T5152	21574-21577	DT	denotes	the
T5154	21578-21582	JJ	denotes	long
T5153	21583-21586	NN	denotes	arm
T5155	21587-21589	IN	denotes	to
T5156	21590-21593	DT	denotes	the
T5158	21594-21599	JJ	denotes	short
T5157	21600-21603	NN	denotes	arm
T5159	21604-21606	IN	denotes	of
T5160	21607-21615	NN	denotes	homology
T5161	21615-21617	,	denotes	,
T5145	21617-21626	VBD	denotes	contained
T5162	21627-21628	DT	denotes	a
T5164	21629-21635	JJ	denotes	unique
T5165	21636-21640	NN	denotes	SalI
T5163	21641-21645	NN	denotes	site
T5166	21646-21651	WRB	denotes	where
T5168	21652-21655	DT	denotes	the
T5170	21656-21659	CD	denotes	1.3
T5172	21659-21660	HYPH	denotes	-
T5171	21660-21662	NN	denotes	kb
T5173	21663-21667	NN	denotes	exon
T5174	21668-21670	CD	denotes	10
T5169	21671-21677	NN	denotes	region
T5175	21678-21681	VBD	denotes	was
T5167	21682-21689	VBN	denotes	deleted
T5176	21689-21690	.	denotes	.
T5280	21692-21702	NN	denotes	Generation
T5281	21703-21705	IN	denotes	of
T5282	21706-21710	NN	denotes	MCAD
T5284	21710-21711	HYPH	denotes	-
T5283	21711-21720	JJ	denotes	deficient
T5285	21721-21725	NNS	denotes	mice
T5286	21725-21726	.	denotes	.
T5287	21726-21929	sentence	denotes	The Acadm insertion vector was linearized by SalI digestion, the site of the 1.3-kb genomic fragment deletion, and electroporated into E14–1 ES cells (a kind gift from R. Kuhn), derived from 129P2 mice.
T5288	21727-21730	DT	denotes	The
T5290	21731-21736	NN	denotes	Acadm
T5291	21737-21746	NN	denotes	insertion
T5289	21747-21753	NN	denotes	vector
T5293	21754-21757	VBD	denotes	was
T5292	21758-21768	VBN	denotes	linearized
T5294	21769-21771	IN	denotes	by
T5295	21772-21776	NN	denotes	SalI
T5296	21777-21786	NN	denotes	digestion
T5297	21786-21788	,	denotes	,
T5298	21788-21791	DT	denotes	the
T5299	21792-21796	NN	denotes	site
T5300	21797-21799	IN	denotes	of
T5301	21800-21803	DT	denotes	the
T5303	21804-21807	CD	denotes	1.3
T5305	21807-21808	HYPH	denotes	-
T5304	21808-21810	NN	denotes	kb
T5306	21811-21818	JJ	denotes	genomic
T5307	21819-21827	NN	denotes	fragment
T5302	21828-21836	NN	denotes	deletion
T5308	21836-21838	,	denotes	,
T5309	21838-21841	CC	denotes	and
T5310	21842-21856	VBN	denotes	electroporated
T5311	21857-21861	IN	denotes	into
T5312	21862-21865	NN	denotes	E14
T5314	21865-21866	HYPH	denotes	–
T5315	21866-21867	CD	denotes	1
T5316	21868-21870	NN	denotes	ES
T5313	21871-21876	NNS	denotes	cells
T5317	21877-21878	-LRB-	denotes	(
T5319	21878-21879	DT	denotes	a
T5320	21880-21884	JJ	denotes	kind
T5318	21885-21889	NN	denotes	gift
T5321	21890-21894	IN	denotes	from
T5322	21895-21897	NNP	denotes	R.
T5323	21898-21902	NNP	denotes	Kuhn
T5324	21902-21903	-RRB-	denotes	)
T5325	21903-21905	,	denotes	,
T5326	21905-21912	VBN	denotes	derived
T5327	21913-21917	IN	denotes	from
T5328	21918-21923	NN	denotes	129P2
T5329	21924-21928	NNS	denotes	mice
T5330	21928-21929	.	denotes	.
T5331	21929-22101	sentence	denotes	Correctly targeted Acadm insertion vector was designed to undergo gap repair of the 1.3-kb deletion upon double stranded–break repair [26] during homologous recombination.
T5332	21930-21939	RB	denotes	Correctly
T5333	21940-21948	VBN	denotes	targeted
T5335	21949-21954	NN	denotes	Acadm
T5336	21955-21964	NN	denotes	insertion
T5334	21965-21971	NN	denotes	vector
T5338	21972-21975	VBD	denotes	was
T5337	21976-21984	VBN	denotes	designed
T5339	21985-21987	TO	denotes	to
T5340	21988-21995	VB	denotes	undergo
T5341	21996-21999	NN	denotes	gap
T5342	22000-22006	NN	denotes	repair
T5343	22007-22009	IN	denotes	of
T5344	22010-22013	DT	denotes	the
T5346	22014-22017	CD	denotes	1.3
T5348	22017-22018	HYPH	denotes	-
T5347	22018-22020	NN	denotes	kb
T5345	22021-22029	NN	denotes	deletion
T5349	22030-22034	IN	denotes	upon
T5350	22035-22041	JJ	denotes	double
T5351	22042-22050	VBN	denotes	stranded
T5353	22050-22051	HYPH	denotes	–
T5352	22051-22056	NN	denotes	break
T5354	22057-22063	NN	denotes	repair
T5355	22064-22065	-LRB-	denotes	[
T5356	22065-22067	CD	denotes	26
T5357	22067-22068	-RRB-	denotes	]
T5358	22069-22075	IN	denotes	during
T5359	22076-22086	JJ	denotes	homologous
T5360	22087-22100	NN	denotes	recombination
T5361	22100-22101	.	denotes	.
T5362	22101-22175	sentence	denotes	Southern blot analysis was conducted to confirm homologous recombination.
T5363	22102-22110	NNP	denotes	Southern
T5364	22111-22115	NN	denotes	blot
T5365	22116-22124	NN	denotes	analysis
T5367	22125-22128	VBD	denotes	was
T5366	22129-22138	VBN	denotes	conducted
T5368	22139-22141	TO	denotes	to
T5369	22142-22149	VB	denotes	confirm
T5370	22150-22160	JJ	denotes	homologous
T5371	22161-22174	NN	denotes	recombination
T5372	22174-22175	.	denotes	.
T5373	22175-22362	sentence	denotes	Genomic DNA from G418 resistant ES colonies was digested with EcoRI, electrophoresed, blotted, and probed with an 850-bp probe (probe A) generated by PCR from Acadm exon 10 to intron 10.
T5374	22176-22183	JJ	denotes	Genomic
T5375	22184-22187	NN	denotes	DNA
T5377	22188-22192	IN	denotes	from
T5378	22193-22197	NN	denotes	G418
T5379	22198-22207	JJ	denotes	resistant
T5381	22208-22210	NN	denotes	ES
T5380	22211-22219	NNS	denotes	colonies
T5382	22220-22223	VBD	denotes	was
T5376	22224-22232	VBN	denotes	digested
T5383	22233-22237	IN	denotes	with
T5384	22238-22243	NN	denotes	EcoRI
T5385	22243-22245	,	denotes	,
T5386	22245-22260	VBN	denotes	electrophoresed
T5387	22260-22262	,	denotes	,
T5388	22262-22269	VBN	denotes	blotted
T5389	22269-22271	,	denotes	,
T5390	22271-22274	CC	denotes	and
T5391	22275-22281	VBN	denotes	probed
T5392	22282-22286	IN	denotes	with
T5393	22287-22289	DT	denotes	an
T5395	22290-22293	CD	denotes	850
T5397	22293-22294	HYPH	denotes	-
T5396	22294-22296	NN	denotes	bp
T5394	22297-22302	NN	denotes	probe
T5398	22303-22304	-LRB-	denotes	(
T5400	22304-22309	NN	denotes	probe
T5399	22310-22311	NN	denotes	A
T5401	22311-22312	-RRB-	denotes	)
T5402	22313-22322	VBN	denotes	generated
T5403	22323-22325	IN	denotes	by
T5404	22326-22329	NN	denotes	PCR
T5405	22330-22334	IN	denotes	from
T5406	22335-22340	NN	denotes	Acadm
T5407	22341-22345	NN	denotes	exon
T5408	22346-22348	CD	denotes	10
T5409	22349-22351	IN	denotes	to
T5410	22352-22358	NN	denotes	intron
T5411	22359-22361	CD	denotes	10
T5412	22361-22362	.	denotes	.
T5413	22362-22520	sentence	denotes	This DNA fragment is not present within Acadm insertion vector and was expected to hybridize to a 13.2-kb genomic DNA fragment upon homologous recombination.
T5414	22363-22367	DT	denotes	This
T5416	22368-22371	NN	denotes	DNA
T5415	22372-22380	NN	denotes	fragment
T5417	22381-22383	VBZ	denotes	is
T5418	22384-22387	RB	denotes	not
T5419	22388-22395	JJ	denotes	present
T5420	22396-22402	IN	denotes	within
T5421	22403-22408	NN	denotes	Acadm
T5423	22409-22418	NN	denotes	insertion
T5422	22419-22425	NN	denotes	vector
T5424	22426-22429	CC	denotes	and
T5425	22430-22433	VBD	denotes	was
T5426	22434-22442	VBN	denotes	expected
T5427	22443-22445	TO	denotes	to
T5428	22446-22455	VB	denotes	hybridize
T5429	22456-22458	IN	denotes	to
T5430	22459-22460	DT	denotes	a
T5432	22461-22465	CD	denotes	13.2
T5434	22465-22466	HYPH	denotes	-
T5433	22466-22468	NN	denotes	kb
T5435	22469-22476	JJ	denotes	genomic
T5436	22477-22480	NN	denotes	DNA
T5431	22481-22489	NN	denotes	fragment
T5437	22490-22494	IN	denotes	upon
T5438	22495-22505	JJ	denotes	homologous
T5439	22506-22519	NN	denotes	recombination
T5440	22519-22520	.	denotes	.
T5441	22520-22620	sentence	denotes	Correctly targeted ES cell clones were microinjected into B6 blastocysts to generate chimeric mice.
T5442	22521-22530	RB	denotes	Correctly
T5443	22531-22539	VBN	denotes	targeted
T5445	22540-22542	NN	denotes	ES
T5446	22543-22547	NN	denotes	cell
T5444	22548-22554	NNS	denotes	clones
T5448	22555-22559	VBD	denotes	were
T5447	22560-22573	VBN	denotes	microinjected
T5449	22574-22578	IN	denotes	into
T5450	22579-22581	NN	denotes	B6
T5451	22582-22593	NNS	denotes	blastocysts
T5452	22594-22596	TO	denotes	to
T5453	22597-22605	VB	denotes	generate
T5454	22606-22614	JJ	denotes	chimeric
T5455	22615-22619	NNS	denotes	mice
T5456	22619-22620	.	denotes	.
T5457	22620-22796	sentence	denotes	Chimeric mice were subsequently backcrossed to B6 and 129P2 mice to produce gene-targeted mice AcadmtmUab1/+ (MCAD+/−) and eventually AcadmtmUab1/tm1Uab MCAD−/− (B6;129) mice.
T5458	22621-22629	JJ	denotes	Chimeric
T5459	22630-22634	NNS	denotes	mice
T5461	22635-22639	VBD	denotes	were
T5462	22640-22652	RB	denotes	subsequently
T5460	22653-22664	VBN	denotes	backcrossed
T5463	22665-22667	IN	denotes	to
T5464	22668-22670	NN	denotes	B6
T5466	22671-22674	CC	denotes	and
T5467	22675-22680	NN	denotes	129P2
T5465	22681-22685	NNS	denotes	mice
T5468	22686-22688	TO	denotes	to
T5469	22689-22696	VB	denotes	produce
T5470	22697-22701	NN	denotes	gene
T5472	22701-22702	HYPH	denotes	-
T5471	22702-22710	VBN	denotes	targeted
T5473	22711-22715	NNS	denotes	mice
T5474	22716-22727	NN	denotes	AcadmtmUab1
T5475	22727-22728	HYPH	denotes	/
T5476	22728-22729	SYM	denotes	+
T5477	22730-22731	-LRB-	denotes	(
T5478	22731-22735	NN	denotes	MCAD
T5479	22735-22736	SYM	denotes	+
T5480	22736-22737	HYPH	denotes	/
T5481	22737-22738	SYM	denotes	−
T5482	22738-22739	-RRB-	denotes	)
T5483	22740-22743	CC	denotes	and
T5484	22744-22754	RB	denotes	eventually
T5486	22755-22766	NN	denotes	AcadmtmUab1
T5488	22766-22767	HYPH	denotes	/
T5487	22767-22773	NN	denotes	tm1Uab
T5489	22774-22778	NN	denotes	MCAD
T5490	22778-22779	SYM	denotes	−
T5491	22779-22780	HYPH	denotes	/
T5492	22780-22781	SYM	denotes	−
T5493	22782-22783	-LRB-	denotes	(
T5494	22783-22785	NN	denotes	B6
T5495	22785-22786	:	denotes	;
T5496	22786-22789	CD	denotes	129
T5497	22789-22790	-RRB-	denotes	)
T5485	22791-22795	NNS	denotes	mice
T5498	22795-22796	.	denotes	.
T5499	22796-23025	sentence	denotes	Mice were negative for mouse pathogens based on serological assays for ten different viruses, aerobic bacterial cultures of nasopharynx and cecum, examinations for endo- and ectoparasites, and histopathology of all major organs.
T5500	22797-22801	NNS	denotes	Mice
T5501	22802-22806	VBD	denotes	were
T5502	22807-22815	JJ	denotes	negative
T5503	22816-22819	IN	denotes	for
T5504	22820-22825	NN	denotes	mouse
T5505	22826-22835	NNS	denotes	pathogens
T5506	22836-22841	VBN	denotes	based
T5507	22842-22844	IN	denotes	on
T5508	22845-22856	JJ	denotes	serological
T5509	22857-22863	NNS	denotes	assays
T5510	22864-22867	IN	denotes	for
T5511	22868-22871	CD	denotes	ten
T5513	22872-22881	JJ	denotes	different
T5512	22882-22889	NNS	denotes	viruses
T5514	22889-22891	,	denotes	,
T5515	22891-22898	JJ	denotes	aerobic
T5517	22899-22908	JJ	denotes	bacterial
T5516	22909-22917	NNS	denotes	cultures
T5518	22918-22920	IN	denotes	of
T5519	22921-22932	NN	denotes	nasopharynx
T5520	22933-22936	CC	denotes	and
T5521	22937-22942	NN	denotes	cecum
T5522	22942-22944	,	denotes	,
T5523	22944-22956	NNS	denotes	examinations
T5524	22957-22960	IN	denotes	for
T5525	22961-22965	AFX	denotes	endo
T5527	22965-22966	HYPH	denotes	-
T5528	22967-22970	CC	denotes	and
T5526	22971-22984	NNS	denotes	ectoparasites
T5529	22984-22986	,	denotes	,
T5530	22986-22989	CC	denotes	and
T5531	22990-23004	NN	denotes	histopathology
T5532	23005-23007	IN	denotes	of
T5533	23008-23011	DT	denotes	all
T5535	23012-23017	JJ	denotes	major
T5534	23018-23024	NNS	denotes	organs
T5536	23024-23025	.	denotes	.
T5679	23027-23030	NN	denotes	RNA
T5680	23031-23039	NN	denotes	analysis
T5681	23039-23040	.	denotes	.
T5682	23040-23193	sentence	denotes	Total RNA was isolated from the heart of 30 day old MCAD+/+, MCAD+/−, and MCAD−/− mice by standard techniques using guanidinium thiocyanate method [27].
T5683	23041-23046	JJ	denotes	Total
T5684	23047-23050	NN	denotes	RNA
T5686	23051-23054	VBD	denotes	was
T5685	23055-23063	VBN	denotes	isolated
T5687	23064-23068	IN	denotes	from
T5688	23069-23072	DT	denotes	the
T5689	23073-23078	NN	denotes	heart
T5690	23079-23081	IN	denotes	of
T5691	23082-23084	CD	denotes	30
T5692	23085-23088	NN	denotes	day
T5693	23089-23092	JJ	denotes	old
T5695	23093-23097	NN	denotes	MCAD
T5696	23097-23098	SYM	denotes	+
T5697	23098-23099	HYPH	denotes	/
T5698	23099-23100	SYM	denotes	+
T5699	23100-23102	,	denotes	,
T5700	23102-23106	NN	denotes	MCAD
T5701	23106-23107	SYM	denotes	+
T5702	23107-23108	HYPH	denotes	/
T5703	23108-23109	SYM	denotes	−
T5704	23109-23111	,	denotes	,
T5705	23111-23114	CC	denotes	and
T5706	23115-23119	NN	denotes	MCAD
T5707	23119-23120	SYM	denotes	−
T5708	23120-23121	HYPH	denotes	/
T5709	23121-23122	SYM	denotes	−
T5694	23123-23127	NNS	denotes	mice
T5710	23128-23130	IN	denotes	by
T5711	23131-23139	JJ	denotes	standard
T5712	23140-23150	NNS	denotes	techniques
T5713	23151-23156	VBG	denotes	using
T5714	23157-23168	NN	denotes	guanidinium
T5716	23169-23180	NN	denotes	thiocyanate
T5715	23181-23187	NN	denotes	method
T5717	23188-23189	-LRB-	denotes	[
T5718	23189-23191	CD	denotes	27
T5719	23191-23192	-RRB-	denotes	]
T5720	23192-23193	.	denotes	.
T5721	23193-23348	sentence	denotes	Reverse transcription was performed using random oligonucleotides as recommended by the manufacturer (Clontech, Mountain View, California, United States).
T5722	23194-23201	JJ	denotes	Reverse
T5723	23202-23215	NN	denotes	transcription
T5725	23216-23219	VBD	denotes	was
T5724	23220-23229	VBN	denotes	performed
T5726	23230-23235	VBG	denotes	using
T5727	23236-23242	JJ	denotes	random
T5728	23243-23259	NNS	denotes	oligonucleotides
T5729	23260-23262	IN	denotes	as
T5730	23263-23274	VBN	denotes	recommended
T5731	23275-23277	IN	denotes	by
T5732	23278-23281	DT	denotes	the
T5733	23282-23294	NN	denotes	manufacturer
T5734	23295-23296	-LRB-	denotes	(
T5735	23296-23304	NNP	denotes	Clontech
T5736	23304-23306	,	denotes	,
T5737	23306-23314	NNP	denotes	Mountain
T5738	23315-23319	NNP	denotes	View
T5739	23319-23321	,	denotes	,
T5740	23321-23331	NNP	denotes	California
T5741	23331-23333	,	denotes	,
T5742	23333-23339	NNP	denotes	United
T5743	23340-23346	NNP	denotes	States
T5744	23346-23347	-RRB-	denotes	)
T5745	23347-23348	.	denotes	.
T5746	23348-23443	sentence	denotes	PCR was subsequently performed using oligonucleotides specific to exon 7 and exon 11 of Acadm.
T5747	23349-23352	NN	denotes	PCR
T5749	23353-23356	VBD	denotes	was
T5750	23357-23369	RB	denotes	subsequently
T5748	23370-23379	VBN	denotes	performed
T5751	23380-23385	VBG	denotes	using
T5752	23386-23402	NNS	denotes	oligonucleotides
T5753	23403-23411	JJ	denotes	specific
T5754	23412-23414	IN	denotes	to
T5755	23415-23419	NN	denotes	exon
T5756	23420-23421	CD	denotes	7
T5757	23422-23425	CC	denotes	and
T5758	23426-23430	NN	denotes	exon
T5759	23431-23433	CD	denotes	11
T5760	23434-23436	IN	denotes	of
T5761	23437-23442	NN	denotes	Acadm
T5762	23442-23443	.	denotes	.
T5763	23443-23497	sentence	denotes	PCR amplifications were performed as described above.
T5764	23444-23447	NN	denotes	PCR
T5765	23448-23462	NNS	denotes	amplifications
T5767	23463-23467	VBD	denotes	were
T5766	23468-23477	VBN	denotes	performed
T5768	23478-23480	IN	denotes	as
T5769	23481-23490	VBN	denotes	described
T5770	23491-23496	RB	denotes	above
T5771	23496-23497	.	denotes	.
T5772	23497-23626	sentence	denotes	PCR fragments were subsequently sequenced after subcloning into pGEM-T Easy vector (Promega, Madison, Wisconsin, United States).
T5773	23498-23501	NN	denotes	PCR
T5774	23502-23511	NNS	denotes	fragments
T5776	23512-23516	VBD	denotes	were
T5777	23517-23529	RB	denotes	subsequently
T5775	23530-23539	VBN	denotes	sequenced
T5778	23540-23545	IN	denotes	after
T5779	23546-23556	VBG	denotes	subcloning
T5780	23557-23561	IN	denotes	into
T5781	23562-23566	NN	denotes	pGEM
T5783	23566-23567	HYPH	denotes	-
T5782	23567-23568	NN	denotes	T
T5785	23569-23573	NNP	denotes	Easy
T5784	23574-23580	NNP	denotes	vector
T5786	23581-23582	-LRB-	denotes	(
T5787	23582-23589	NNP	denotes	Promega
T5788	23589-23591	,	denotes	,
T5789	23591-23598	NNP	denotes	Madison
T5790	23598-23600	,	denotes	,
T5791	23600-23609	NNP	denotes	Wisconsin
T5792	23609-23611	,	denotes	,
T5793	23611-23617	NNP	denotes	United
T5794	23618-23624	NNP	denotes	States
T5795	23624-23625	-RRB-	denotes	)
T5796	23625-23626	.	denotes	.
T5797	23626-23744	sentence	denotes	In order to determine the extent of Acadm mRNA expressed from the MCAD−/− mice, northern blot analysis was performed.
T5798	23627-23629	IN	denotes	In
T5800	23630-23635	NN	denotes	order
T5801	23636-23638	TO	denotes	to
T5802	23639-23648	VB	denotes	determine
T5803	23649-23652	DT	denotes	the
T5804	23653-23659	NN	denotes	extent
T5805	23660-23662	IN	denotes	of
T5806	23663-23668	NN	denotes	Acadm
T5807	23669-23673	NN	denotes	mRNA
T5808	23674-23683	VBN	denotes	expressed
T5809	23684-23688	IN	denotes	from
T5810	23689-23692	DT	denotes	the
T5812	23693-23697	NN	denotes	MCAD
T5813	23697-23698	SYM	denotes	−
T5814	23698-23699	HYPH	denotes	/
T5815	23699-23700	SYM	denotes	−
T5811	23701-23705	NNS	denotes	mice
T5816	23705-23707	,	denotes	,
T5817	23707-23715	NNP	denotes	northern
T5818	23716-23720	NN	denotes	blot
T5819	23721-23729	NN	denotes	analysis
T5820	23730-23733	VBD	denotes	was
T5799	23734-23743	VBN	denotes	performed
T5821	23743-23744	.	denotes	.
T5822	23744-24017	sentence	denotes	Total RNA was isolated from heart, liver, brown fat, brain, kidney, skeletal muscle, white fat, and testes of 3-mo-old MCAD+/+ and MCAD−/− mice using the Ultraspec RNA Isolation Kit (BIOTEX Laboratories, Inc., Houston, Texas, United States) as per manufacturer's protocol.
T5823	23745-23750	JJ	denotes	Total
T5824	23751-23754	NN	denotes	RNA
T5826	23755-23758	VBD	denotes	was
T5825	23759-23767	VBN	denotes	isolated
T5827	23768-23772	IN	denotes	from
T5828	23773-23778	NN	denotes	heart
T5829	23778-23780	,	denotes	,
T5830	23780-23785	NN	denotes	liver
T5831	23785-23787	,	denotes	,
T5832	23787-23792	JJ	denotes	brown
T5833	23793-23796	NN	denotes	fat
T5834	23796-23798	,	denotes	,
T5835	23798-23803	NN	denotes	brain
T5836	23803-23805	,	denotes	,
T5837	23805-23811	NN	denotes	kidney
T5838	23811-23813	,	denotes	,
T5839	23813-23821	JJ	denotes	skeletal
T5840	23822-23828	NN	denotes	muscle
T5841	23828-23830	,	denotes	,
T5842	23830-23835	JJ	denotes	white
T5843	23836-23839	NN	denotes	fat
T5844	23839-23841	,	denotes	,
T5845	23841-23844	CC	denotes	and
T5846	23845-23851	NNS	denotes	testes
T5847	23852-23854	IN	denotes	of
T5848	23855-23856	CD	denotes	3
T5850	23856-23857	HYPH	denotes	-
T5849	23857-23859	NN	denotes	mo
T5852	23859-23860	HYPH	denotes	-
T5851	23860-23863	JJ	denotes	old
T5854	23864-23868	NN	denotes	MCAD
T5855	23868-23869	SYM	denotes	+
T5856	23869-23870	HYPH	denotes	/
T5857	23870-23871	SYM	denotes	+
T5858	23872-23875	CC	denotes	and
T5859	23876-23880	NN	denotes	MCAD
T5860	23880-23881	SYM	denotes	−
T5861	23881-23882	HYPH	denotes	/
T5862	23882-23883	SYM	denotes	−
T5853	23884-23888	NNS	denotes	mice
T5863	23889-23894	VBG	denotes	using
T5864	23895-23898	DT	denotes	the
T5866	23899-23908	NNP	denotes	Ultraspec
T5867	23909-23912	NN	denotes	RNA
T5868	23913-23922	NN	denotes	Isolation
T5865	23923-23926	NN	denotes	Kit
T5869	23927-23928	-LRB-	denotes	(
T5871	23928-23934	NNP	denotes	BIOTEX
T5870	23935-23947	NNP	denotes	Laboratories
T5872	23947-23949	,	denotes	,
T5873	23949-23953	NNP	denotes	Inc.
T5874	23953-23955	,	denotes	,
T5875	23955-23962	NNP	denotes	Houston
T5876	23962-23964	,	denotes	,
T5877	23964-23969	NNP	denotes	Texas
T5878	23969-23971	,	denotes	,
T5879	23971-23977	NNP	denotes	United
T5880	23978-23984	NNP	denotes	States
T5881	23984-23985	-RRB-	denotes	)
T5882	23986-23988	IN	denotes	as
T5883	23989-23992	IN	denotes	per
T5884	23993-24005	NN	denotes	manufacturer
T5886	24005-24007	POS	denotes	's
T5885	24008-24016	NN	denotes	protocol
T5887	24016-24017	.	denotes	.
T5888	24017-24337	sentence	denotes	Ten μg of total RNA from each sample was loaded onto a 0.8% agarose-formaldehyde gel, transferred to nitrocellulose filter (Hybond N; GE Healthcare Amersham Biosciences Corp., Piscataway, New Jersey, United States), and hybridized with 32P-radiolabeled full-length mouse Acadm cDNA probe using standard procedures [28].
T5889	24018-24021	CD	denotes	Ten
T5890	24022-24024	NN	denotes	μg
T5892	24025-24027	IN	denotes	of
T5893	24028-24033	JJ	denotes	total
T5894	24034-24037	NN	denotes	RNA
T5895	24038-24042	IN	denotes	from
T5896	24043-24047	DT	denotes	each
T5897	24048-24054	NN	denotes	sample
T5898	24055-24058	VBD	denotes	was
T5891	24059-24065	VBN	denotes	loaded
T5899	24066-24070	IN	denotes	onto
T5900	24071-24072	DT	denotes	a
T5902	24073-24076	CD	denotes	0.8
T5903	24076-24077	NN	denotes	%
T5905	24078-24085	NN	denotes	agarose
T5906	24085-24086	HYPH	denotes	-
T5904	24086-24098	NN	denotes	formaldehyde
T5901	24099-24102	NN	denotes	gel
T5907	24102-24104	,	denotes	,
T5908	24104-24115	VBN	denotes	transferred
T5909	24116-24118	IN	denotes	to
T5910	24119-24133	NN	denotes	nitrocellulose
T5911	24134-24140	NN	denotes	filter
T5912	24141-24142	-LRB-	denotes	(
T5914	24142-24148	NNP	denotes	Hybond
T5915	24149-24150	NNP	denotes	N
T5916	24150-24151	:	denotes	;
T5917	24152-24154	NNP	denotes	GE
T5918	24155-24165	NNP	denotes	Healthcare
T5919	24166-24174	NNP	denotes	Amersham
T5920	24175-24186	NNP	denotes	Biosciences
T5913	24187-24192	NNP	denotes	Corp.
T5921	24192-24194	,	denotes	,
T5922	24194-24204	NNP	denotes	Piscataway
T5923	24204-24206	,	denotes	,
T5924	24206-24209	NNP	denotes	New
T5925	24210-24216	NNP	denotes	Jersey
T5926	24216-24218	,	denotes	,
T5927	24218-24224	NNP	denotes	United
T5928	24225-24231	NNP	denotes	States
T5929	24231-24232	-RRB-	denotes	)
T5930	24232-24234	,	denotes	,
T5931	24234-24237	CC	denotes	and
T5932	24238-24248	VBN	denotes	hybridized
T5933	24249-24253	IN	denotes	with
T5934	24254-24257	NN	denotes	32P
T5936	24257-24258	HYPH	denotes	-
T5935	24258-24270	VBN	denotes	radiolabeled
T5938	24271-24275	JJ	denotes	full
T5940	24275-24276	HYPH	denotes	-
T5939	24276-24282	NN	denotes	length
T5941	24283-24288	NN	denotes	mouse
T5942	24289-24294	NN	denotes	Acadm
T5943	24295-24299	NN	denotes	cDNA
T5937	24300-24305	NN	denotes	probe
T5944	24306-24311	VBG	denotes	using
T5945	24312-24320	JJ	denotes	standard
T5946	24321-24331	NNS	denotes	procedures
T5947	24332-24333	-LRB-	denotes	[
T5948	24333-24335	CD	denotes	28
T5949	24335-24336	-RRB-	denotes	]
T5950	24336-24337	.	denotes	.
T5951	24337-24518	sentence	denotes	Hybridizations were performed under highly stringent conditions (42 °C in 2× SSC, 50% formamide, 10% dextran sulfate, 5× Denhardt's reagent, 1% SDS, and salmon sperm DNA) for 18 h.
T5952	24338-24352	NNS	denotes	Hybridizations
T5954	24353-24357	VBD	denotes	were
T5953	24358-24367	VBN	denotes	performed
T5955	24368-24373	IN	denotes	under
T5956	24374-24380	RB	denotes	highly
T5957	24381-24390	JJ	denotes	stringent
T5958	24391-24401	NNS	denotes	conditions
T5959	24402-24403	-LRB-	denotes	(
T5961	24403-24405	CD	denotes	42
T5960	24406-24408	NN	denotes	°C
T5962	24409-24411	IN	denotes	in
T5963	24412-24413	CD	denotes	2
T5965	24413-24414	SYM	denotes	×
T5964	24415-24418	NN	denotes	SSC
T5966	24418-24420	,	denotes	,
T5967	24420-24422	CD	denotes	50
T5968	24422-24423	NN	denotes	%
T5969	24424-24433	NN	denotes	formamide
T5970	24433-24435	,	denotes	,
T5971	24435-24437	CD	denotes	10
T5972	24437-24438	NN	denotes	%
T5974	24439-24446	NN	denotes	dextran
T5973	24447-24454	NN	denotes	sulfate
T5975	24454-24456	,	denotes	,
T5976	24456-24457	CD	denotes	5
T5978	24457-24458	SYM	denotes	×
T5979	24459-24467	NNP	denotes	Denhardt
T5980	24467-24469	POS	denotes	's
T5977	24470-24477	NN	denotes	reagent
T5981	24477-24479	,	denotes	,
T5982	24479-24480	CD	denotes	1
T5983	24480-24481	NN	denotes	%
T5984	24482-24485	NN	denotes	SDS
T5985	24485-24487	,	denotes	,
T5986	24487-24490	CC	denotes	and
T5987	24491-24497	NN	denotes	salmon
T5988	24498-24503	NN	denotes	sperm
T5989	24504-24507	NN	denotes	DNA
T5990	24507-24508	-RRB-	denotes	)
T5991	24509-24512	IN	denotes	for
T5992	24513-24515	CD	denotes	18
T5993	24516-24517	NN	denotes	h
T5994	24517-24518	.	denotes	.
T5995	24518-24633	sentence	denotes	The hybridized filter was washed two times in 4× SSC, 0.1% SDS and two times in 2× SSC; 0.1% SDS at 55 °C for 1 h.
T5996	24519-24522	DT	denotes	The
T5998	24523-24533	JJ	denotes	hybridized
T5997	24534-24540	NN	denotes	filter
T6000	24541-24544	VBD	denotes	was
T5999	24545-24551	VBN	denotes	washed
T6001	24552-24555	CD	denotes	two
T6002	24556-24561	NNS	denotes	times
T6003	24562-24564	IN	denotes	in
T6004	24565-24566	CD	denotes	4
T6006	24566-24567	SYM	denotes	×
T6005	24568-24571	NN	denotes	SSC
T6007	24571-24573	,	denotes	,
T6008	24573-24576	CD	denotes	0.1
T6009	24576-24577	NN	denotes	%
T6010	24578-24581	NN	denotes	SDS
T6011	24582-24585	CC	denotes	and
T6012	24586-24589	CD	denotes	two
T6013	24590-24595	NNS	denotes	times
T6014	24596-24598	IN	denotes	in
T6015	24599-24600	CD	denotes	2
T6017	24600-24601	SYM	denotes	×
T6016	24602-24605	NN	denotes	SSC
T6018	24605-24606	:	denotes	;
T6019	24607-24610	CD	denotes	0.1
T6020	24610-24611	NN	denotes	%
T6021	24612-24615	NN	denotes	SDS
T6022	24616-24618	IN	denotes	at
T6023	24619-24621	CD	denotes	55
T6024	24622-24624	NNS	denotes	°C
T6025	24625-24628	IN	denotes	for
T6026	24629-24630	CD	denotes	1
T6027	24631-24632	NN	denotes	h
T6028	24632-24633	.	denotes	.
T6029	24633-24772	sentence	denotes	The filter was exposed to autoradiographic film (Hyperfilm MP; GE Healthcare Amersham Biosciences, Piscataway, New Jersey, United States).
T6030	24634-24637	DT	denotes	The
T6031	24638-24644	NN	denotes	filter
T6033	24645-24648	VBD	denotes	was
T6032	24649-24656	VBN	denotes	exposed
T6034	24657-24659	IN	denotes	to
T6035	24660-24676	JJ	denotes	autoradiographic
T6036	24677-24681	NN	denotes	film
T6037	24682-24683	-LRB-	denotes	(
T6039	24683-24692	NNP	denotes	Hyperfilm
T6040	24693-24695	NNP	denotes	MP
T6041	24695-24696	:	denotes	;
T6042	24697-24699	NNP	denotes	GE
T6043	24700-24710	NNP	denotes	Healthcare
T6044	24711-24719	NNP	denotes	Amersham
T6038	24720-24731	NNP	denotes	Biosciences
T6045	24731-24733	,	denotes	,
T6046	24733-24743	NNP	denotes	Piscataway
T6047	24743-24745	,	denotes	,
T6048	24745-24748	NNP	denotes	New
T6049	24749-24755	NNP	denotes	Jersey
T6050	24755-24757	,	denotes	,
T6051	24757-24763	NNP	denotes	United
T6052	24764-24770	NNP	denotes	States
T6053	24770-24771	-RRB-	denotes	)
T6054	24771-24772	.	denotes	.
T6055	24772-24870	sentence	denotes	Replicate agarose-formaldehyde gels were stained by ethidium bromide to verify equal RNA loading.
T6056	24773-24782	JJ	denotes	Replicate
T6058	24783-24790	NN	denotes	agarose
T6060	24790-24791	HYPH	denotes	-
T6059	24791-24803	NN	denotes	formaldehyde
T6057	24804-24808	NNS	denotes	gels
T6062	24809-24813	VBD	denotes	were
T6061	24814-24821	VBN	denotes	stained
T6063	24822-24824	IN	denotes	by
T6064	24825-24833	NN	denotes	ethidium
T6065	24834-24841	NN	denotes	bromide
T6066	24842-24844	TO	denotes	to
T6067	24845-24851	VB	denotes	verify
T6068	24852-24857	JJ	denotes	equal
T6070	24858-24861	NN	denotes	RNA
T6069	24862-24869	NN	denotes	loading
T6071	24869-24870	.	denotes	.
T6221	24872-24882	NN	denotes	Immunoblot
T6222	24883-24891	NN	denotes	analysis
T6223	24892-24894	IN	denotes	of
T6224	24895-24899	NN	denotes	MCAD
T6225	24900-24907	NN	denotes	protein
T6226	24907-24908	.	denotes	.
T6227	24908-25102	sentence	denotes	To evaluate the quantity of MCAD protein in mouse tissue, liver samples from 6–8-wk-old MCAD+/+ (n = 1) and MCAD−/− (n = 3) mice were immediately frozen in liquid nitrogen and stored at −80 °C.
T6228	24909-24911	TO	denotes	To
T6229	24912-24920	VB	denotes	evaluate
T6231	24921-24924	DT	denotes	the
T6232	24925-24933	NN	denotes	quantity
T6233	24934-24936	IN	denotes	of
T6234	24937-24941	NN	denotes	MCAD
T6235	24942-24949	NN	denotes	protein
T6236	24950-24952	IN	denotes	in
T6237	24953-24958	NN	denotes	mouse
T6238	24959-24965	NN	denotes	tissue
T6239	24965-24967	,	denotes	,
T6240	24967-24972	NN	denotes	liver
T6241	24973-24980	NNS	denotes	samples
T6242	24981-24985	IN	denotes	from
T6243	24986-24987	CD	denotes	6
T6245	24987-24988	SYM	denotes	–
T6244	24988-24989	CD	denotes	8
T6247	24989-24990	HYPH	denotes	-
T6246	24990-24992	NN	denotes	wk
T6249	24992-24993	HYPH	denotes	-
T6248	24993-24996	JJ	denotes	old
T6251	24997-25001	NN	denotes	MCAD
T6252	25001-25002	SYM	denotes	+
T6253	25002-25003	HYPH	denotes	/
T6254	25003-25004	SYM	denotes	+
T6255	25005-25006	-LRB-	denotes	(
T6257	25006-25007	NN	denotes	n
T6258	25008-25009	SYM	denotes	=
T6256	25010-25011	CD	denotes	1
T6259	25011-25012	-RRB-	denotes	)
T6260	25013-25016	CC	denotes	and
T6261	25017-25021	NN	denotes	MCAD
T6262	25021-25022	SYM	denotes	−
T6263	25022-25023	HYPH	denotes	/
T6264	25023-25024	SYM	denotes	−
T6265	25025-25026	-LRB-	denotes	(
T6267	25026-25027	NN	denotes	n
T6268	25028-25029	SYM	denotes	=
T6266	25030-25031	CD	denotes	3
T6269	25031-25032	-RRB-	denotes	)
T6250	25033-25037	NNS	denotes	mice
T6270	25038-25042	VBD	denotes	were
T6271	25043-25054	RB	denotes	immediately
T6230	25055-25061	VBN	denotes	frozen
T6272	25062-25064	IN	denotes	in
T6273	25065-25071	JJ	denotes	liquid
T6274	25072-25080	NN	denotes	nitrogen
T6275	25081-25084	CC	denotes	and
T6276	25085-25091	VBN	denotes	stored
T6277	25092-25094	IN	denotes	at
T6278	25095-25096	SYM	denotes	−
T6279	25096-25098	CD	denotes	80
T6280	25099-25101	NN	denotes	°C
T6281	25101-25102	.	denotes	.
T6282	25102-25422	sentence	denotes	For analysis, tissue was homogenized and lysed in RIPA buffer (1× PBS, 1% Nonidet P-40, 0.5% sodium deoxycholate, and 1% SDS) with 10% glycerol and Complete Protease Inhibitor, (Roche Diagnostics Corporation, Indianapolis, Indiana, United States), 1 mM of phenylmethylsulfonylfluoride, and 1 mM of sodium orthovanadate.
T6283	25103-25106	IN	denotes	For
T6285	25107-25115	NN	denotes	analysis
T6286	25115-25117	,	denotes	,
T6287	25117-25123	NN	denotes	tissue
T6288	25124-25127	VBD	denotes	was
T6284	25128-25139	VBN	denotes	homogenized
T6289	25140-25143	CC	denotes	and
T6290	25144-25149	VBN	denotes	lysed
T6291	25150-25152	IN	denotes	in
T6292	25153-25157	NN	denotes	RIPA
T6293	25158-25164	NN	denotes	buffer
T6294	25165-25166	-LRB-	denotes	(
T6296	25166-25167	CD	denotes	1
T6297	25167-25168	SYM	denotes	×
T6295	25169-25172	NN	denotes	PBS
T6298	25172-25174	,	denotes	,
T6299	25174-25175	CD	denotes	1
T6300	25175-25176	NN	denotes	%
T6302	25177-25184	NN	denotes	Nonidet
T6301	25185-25186	NN	denotes	P
T6303	25186-25187	HYPH	denotes	-
T6304	25187-25189	CD	denotes	40
T6305	25189-25191	,	denotes	,
T6306	25191-25194	CD	denotes	0.5
T6307	25194-25195	NN	denotes	%
T6309	25196-25202	NN	denotes	sodium
T6308	25203-25215	NN	denotes	deoxycholate
T6310	25215-25217	,	denotes	,
T6311	25217-25220	CC	denotes	and
T6312	25221-25222	CD	denotes	1
T6313	25222-25223	NN	denotes	%
T6314	25224-25227	NN	denotes	SDS
T6315	25227-25228	-RRB-	denotes	)
T6316	25229-25233	IN	denotes	with
T6317	25234-25236	CD	denotes	10
T6318	25236-25237	NN	denotes	%
T6319	25238-25246	NN	denotes	glycerol
T6320	25247-25250	CC	denotes	and
T6321	25251-25259	JJ	denotes	Complete
T6323	25260-25268	NN	denotes	Protease
T6322	25269-25278	NN	denotes	Inhibitor
T6324	25278-25280	,	denotes	,
T6325	25280-25281	-LRB-	denotes	(
T6327	25281-25286	NNP	denotes	Roche
T6328	25287-25298	NNP	denotes	Diagnostics
T6326	25299-25310	NNP	denotes	Corporation
T6329	25310-25312	,	denotes	,
T6330	25312-25324	NNP	denotes	Indianapolis
T6331	25324-25326	,	denotes	,
T6332	25326-25333	NNP	denotes	Indiana
T6333	25333-25335	,	denotes	,
T6334	25335-25341	NNP	denotes	United
T6335	25342-25348	NNP	denotes	States
T6336	25348-25349	-RRB-	denotes	)
T6337	25349-25351	,	denotes	,
T6338	25351-25352	CD	denotes	1
T6339	25353-25355	NN	denotes	mM
T6340	25356-25358	IN	denotes	of
T6341	25359-25387	NN	denotes	phenylmethylsulfonylfluoride
T6342	25387-25389	,	denotes	,
T6343	25389-25392	CC	denotes	and
T6344	25393-25394	CD	denotes	1
T6345	25395-25397	NN	denotes	mM
T6346	25398-25400	IN	denotes	of
T6347	25401-25407	NN	denotes	sodium
T6348	25408-25421	NN	denotes	orthovanadate
T6349	25421-25422	.	denotes	.
T6350	25422-25524	sentence	denotes	Lysates were quantified by Bradford BCA protein assay (Bio-Rad, Hercules, California, United States).
T6351	25423-25430	NNS	denotes	Lysates
T6353	25431-25435	VBD	denotes	were
T6352	25436-25446	VBN	denotes	quantified
T6354	25447-25449	IN	denotes	by
T6355	25450-25458	NNP	denotes	Bradford
T6357	25459-25462	NN	denotes	BCA
T6358	25463-25470	NN	denotes	protein
T6356	25471-25476	NN	denotes	assay
T6359	25477-25478	-LRB-	denotes	(
T6361	25478-25481	NNP	denotes	Bio
T6362	25481-25482	HYPH	denotes	-
T6360	25482-25485	NNP	denotes	Rad
T6363	25485-25487	,	denotes	,
T6364	25487-25495	NNP	denotes	Hercules
T6365	25495-25497	,	denotes	,
T6366	25497-25507	NNP	denotes	California
T6367	25507-25509	,	denotes	,
T6368	25509-25515	NNP	denotes	United
T6369	25516-25522	NNP	denotes	States
T6370	25522-25523	-RRB-	denotes	)
T6371	25523-25524	.	denotes	.
T6372	25524-25710	sentence	denotes	Protein lysates were denatured, separated in 8% SDS-PAGE, and transferred overnight onto a 0.45 μm nitrocellulose membrane (Schleicher and Schuell, Keene, New Hampshire, United States).
T6373	25525-25532	NN	denotes	Protein
T6374	25533-25540	NNS	denotes	lysates
T6376	25541-25545	VBD	denotes	were
T6375	25546-25555	VBN	denotes	denatured
T6377	25555-25557	,	denotes	,
T6378	25557-25566	VBN	denotes	separated
T6379	25567-25569	IN	denotes	in
T6380	25570-25571	CD	denotes	8
T6381	25571-25572	NN	denotes	%
T6383	25573-25576	NN	denotes	SDS
T6384	25576-25577	HYPH	denotes	-
T6382	25577-25581	NN	denotes	PAGE
T6385	25581-25583	,	denotes	,
T6386	25583-25586	CC	denotes	and
T6387	25587-25598	VBN	denotes	transferred
T6388	25599-25608	RB	denotes	overnight
T6389	25609-25613	IN	denotes	onto
T6390	25614-25615	DT	denotes	a
T6392	25616-25620	CD	denotes	0.45
T6393	25621-25623	NN	denotes	μm
T6394	25624-25638	NN	denotes	nitrocellulose
T6391	25639-25647	NN	denotes	membrane
T6395	25648-25649	-LRB-	denotes	(
T6396	25649-25659	NNP	denotes	Schleicher
T6397	25660-25663	CC	denotes	and
T6398	25664-25671	NNP	denotes	Schuell
T6399	25671-25673	,	denotes	,
T6400	25673-25678	NNP	denotes	Keene
T6401	25678-25680	,	denotes	,
T6402	25680-25683	NNP	denotes	New
T6403	25684-25693	NNP	denotes	Hampshire
T6404	25693-25695	,	denotes	,
T6405	25695-25701	NNP	denotes	United
T6406	25702-25708	NNP	denotes	States
T6407	25708-25709	-RRB-	denotes	)
T6408	25709-25710	.	denotes	.
T6409	25710-25889	sentence	denotes	After blocking with 5% nonfat milk in phosphate-buffered saline with 0.1% Tween-20, the membrane was immunoblotted overnight at 4 °C with 1:500 dilution of an anti-MCAD antibody.
T6410	25711-25716	IN	denotes	After
T6412	25717-25725	VBG	denotes	blocking
T6413	25726-25730	IN	denotes	with
T6414	25731-25732	CD	denotes	5
T6415	25732-25733	NN	denotes	%
T6417	25734-25740	JJ	denotes	nonfat
T6416	25741-25745	NN	denotes	milk
T6418	25746-25748	IN	denotes	in
T6419	25749-25758	NN	denotes	phosphate
T6421	25758-25759	HYPH	denotes	-
T6420	25759-25767	VBN	denotes	buffered
T6422	25768-25774	NN	denotes	saline
T6423	25775-25779	IN	denotes	with
T6424	25780-25783	CD	denotes	0.1
T6425	25783-25784	NN	denotes	%
T6426	25785-25790	NN	denotes	Tween
T6427	25790-25791	HYPH	denotes	-
T6428	25791-25793	CD	denotes	20
T6429	25793-25795	,	denotes	,
T6430	25795-25798	DT	denotes	the
T6431	25799-25807	NN	denotes	membrane
T6432	25808-25811	VBD	denotes	was
T6411	25812-25825	VBN	denotes	immunoblotted
T6433	25826-25835	RB	denotes	overnight
T6434	25836-25838	IN	denotes	at
T6435	25839-25840	CD	denotes	4
T6436	25841-25843	NN	denotes	°C
T6437	25844-25848	IN	denotes	with
T6438	25849-25850	CD	denotes	1
T6440	25850-25851	SYM	denotes	:
T6439	25851-25854	CD	denotes	500
T6441	25855-25863	NN	denotes	dilution
T6442	25864-25866	IN	denotes	of
T6443	25867-25869	DT	denotes	an
T6445	25870-25879	JJ	denotes	anti-MCAD
T6444	25880-25888	NN	denotes	antibody
T6446	25888-25889	.	denotes	.
T6447	25889-26133	sentence	denotes	Blots were incubated in anti-mouse IgG HRP-conjugated secondary antibody for 2–4 h at room temperature using standard procedures and developed by chemiluminiscence (Renaissance, NEN Lifesciences Products, Boston, Massachusetts, United States).
T6448	25890-25895	NNS	denotes	Blots
T6450	25896-25900	VBD	denotes	were
T6449	25901-25910	VBN	denotes	incubated
T6451	25911-25913	IN	denotes	in
T6452	25914-25924	JJ	denotes	anti-mouse
T6454	25925-25928	NN	denotes	IgG
T6455	25929-25932	NN	denotes	HRP
T6457	25932-25933	HYPH	denotes	-
T6456	25933-25943	VBN	denotes	conjugated
T6458	25944-25953	JJ	denotes	secondary
T6453	25954-25962	NN	denotes	antibody
T6459	25963-25966	IN	denotes	for
T6460	25967-25968	CD	denotes	2
T6462	25968-25969	SYM	denotes	–
T6461	25969-25970	CD	denotes	4
T6463	25971-25972	NN	denotes	h
T6464	25973-25975	IN	denotes	at
T6465	25976-25980	NN	denotes	room
T6466	25981-25992	NN	denotes	temperature
T6467	25993-25998	VBG	denotes	using
T6468	25999-26007	JJ	denotes	standard
T6469	26008-26018	NNS	denotes	procedures
T6470	26019-26022	CC	denotes	and
T6471	26023-26032	VBN	denotes	developed
T6472	26033-26035	IN	denotes	by
T6473	26036-26053	NN	denotes	chemiluminiscence
T6474	26054-26055	-LRB-	denotes	(
T6475	26055-26066	NNP	denotes	Renaissance
T6476	26066-26068	,	denotes	,
T6477	26068-26071	NNP	denotes	NEN
T6479	26072-26084	NNP	denotes	Lifesciences
T6478	26085-26093	NNP	denotes	Products
T6480	26093-26095	,	denotes	,
T6481	26095-26101	NNP	denotes	Boston
T6482	26101-26103	,	denotes	,
T6483	26103-26116	NNP	denotes	Massachusetts
T6484	26116-26118	,	denotes	,
T6485	26118-26124	NNP	denotes	United
T6486	26125-26131	NNP	denotes	States
T6487	26131-26132	-RRB-	denotes	)
T6488	26132-26133	.	denotes	.
T6565	26135-26140	NN	denotes	Liver
T6566	26141-26147	NN	denotes	enzyme
T6567	26148-26156	NN	denotes	activity
T6568	26156-26157	.	denotes	.
T6569	26157-26280	sentence	denotes	In order to evaluate MCAD activity in mice, liver homogenates were prepared from MCAD+/+ (n = 5) and MCAD−/− mice (n = 5).
T6570	26158-26160	IN	denotes	In
T6572	26161-26166	NN	denotes	order
T6573	26167-26169	TO	denotes	to
T6574	26170-26178	VB	denotes	evaluate
T6575	26179-26183	NN	denotes	MCAD
T6576	26184-26192	NN	denotes	activity
T6577	26193-26195	IN	denotes	in
T6578	26196-26200	NNS	denotes	mice
T6579	26200-26202	,	denotes	,
T6580	26202-26207	NN	denotes	liver
T6581	26208-26219	NNS	denotes	homogenates
T6582	26220-26224	VBD	denotes	were
T6571	26225-26233	VBN	denotes	prepared
T6583	26234-26238	IN	denotes	from
T6584	26239-26243	NN	denotes	MCAD
T6586	26243-26244	SYM	denotes	+
T6587	26244-26245	HYPH	denotes	/
T6588	26245-26246	SYM	denotes	+
T6589	26247-26248	-LRB-	denotes	(
T6591	26248-26249	NN	denotes	n
T6592	26250-26251	SYM	denotes	=
T6590	26252-26253	CD	denotes	5
T6593	26253-26254	-RRB-	denotes	)
T6594	26255-26258	CC	denotes	and
T6595	26259-26263	NN	denotes	MCAD
T6596	26263-26264	SYM	denotes	−
T6597	26264-26265	HYPH	denotes	/
T6598	26265-26266	SYM	denotes	−
T6585	26267-26271	NNS	denotes	mice
T6599	26272-26273	-LRB-	denotes	(
T6601	26273-26274	NN	denotes	n
T6602	26275-26276	SYM	denotes	=
T6600	26277-26278	CD	denotes	5
T6603	26278-26279	-RRB-	denotes	)
T6604	26279-26280	.	denotes	.
T6605	26280-26462	sentence	denotes	The sensitive and highly specific anaerobic ETF reduction assay was used on tissue extracts with octanoyl-CoA (C8) and palmitoyl-CoA (C16) as substrate as previously described [29].
T6606	26281-26284	DT	denotes	The
T6608	26285-26294	JJ	denotes	sensitive
T6609	26295-26298	CC	denotes	and
T6610	26299-26305	RB	denotes	highly
T6611	26306-26314	JJ	denotes	specific
T6612	26315-26324	JJ	denotes	anaerobic
T6613	26325-26328	NN	denotes	ETF
T6614	26329-26338	NN	denotes	reduction
T6607	26339-26344	NN	denotes	assay
T6616	26345-26348	VBD	denotes	was
T6615	26349-26353	VBN	denotes	used
T6617	26354-26356	IN	denotes	on
T6618	26357-26363	NN	denotes	tissue
T6619	26364-26372	NNS	denotes	extracts
T6620	26373-26377	IN	denotes	with
T6621	26378-26386	NN	denotes	octanoyl
T6623	26386-26387	HYPH	denotes	-
T6622	26387-26390	NN	denotes	CoA
T6624	26391-26392	-LRB-	denotes	(
T6625	26392-26394	NN	denotes	C8
T6626	26394-26395	-RRB-	denotes	)
T6627	26396-26399	CC	denotes	and
T6628	26400-26409	NN	denotes	palmitoyl
T6630	26409-26410	HYPH	denotes	-
T6629	26410-26413	NN	denotes	CoA
T6631	26414-26415	-LRB-	denotes	(
T6632	26415-26418	NN	denotes	C16
T6633	26418-26419	-RRB-	denotes	)
T6634	26420-26422	IN	denotes	as
T6635	26423-26432	NN	denotes	substrate
T6636	26433-26435	IN	denotes	as
T6638	26436-26446	RB	denotes	previously
T6637	26447-26456	VBN	denotes	described
T6639	26457-26458	-LRB-	denotes	[
T6640	26458-26460	CD	denotes	29
T6641	26460-26461	-RRB-	denotes	]
T6642	26461-26462	.	denotes	.
T6682	26464-26471	NN	denotes	Fasting
T6683	26472-26475	CC	denotes	and
T6684	26476-26480	NN	denotes	cold
T6685	26481-26490	NN	denotes	challenge
T6686	26490-26491	.	denotes	.
T6687	26491-26680	sentence	denotes	Eight-wk-old MCAD+/+ and MCAD−/− mice were fasted for 18 h (cold tolerance experiments) or 24 h (serum glucose, free fatty acid, organic acid, and carnitine experiments) prior to analysis.
T6688	26492-26497	CD	denotes	Eight
T6690	26497-26498	HYPH	denotes	-
T6689	26498-26500	NN	denotes	wk
T6692	26500-26501	HYPH	denotes	-
T6691	26501-26504	JJ	denotes	old
T6694	26505-26509	NN	denotes	MCAD
T6695	26509-26510	SYM	denotes	+
T6696	26510-26511	HYPH	denotes	/
T6697	26511-26512	SYM	denotes	+
T6698	26513-26516	CC	denotes	and
T6699	26517-26521	NN	denotes	MCAD
T6700	26521-26522	SYM	denotes	−
T6701	26522-26523	HYPH	denotes	/
T6702	26523-26524	SYM	denotes	−
T6693	26525-26529	NNS	denotes	mice
T6704	26530-26534	VBD	denotes	were
T6703	26535-26541	VBN	denotes	fasted
T6705	26542-26545	IN	denotes	for
T6706	26546-26548	CD	denotes	18
T6707	26549-26550	NN	denotes	h
T6708	26551-26552	-LRB-	denotes	(
T6710	26552-26556	NN	denotes	cold
T6711	26557-26566	NN	denotes	tolerance
T6709	26567-26578	NNS	denotes	experiments
T6712	26578-26579	-RRB-	denotes	)
T6713	26580-26582	CC	denotes	or
T6714	26583-26585	CD	denotes	24
T6715	26586-26587	NN	denotes	h
T6716	26588-26589	-LRB-	denotes	(
T6718	26589-26594	NN	denotes	serum
T6719	26595-26602	NN	denotes	glucose
T6720	26602-26604	,	denotes	,
T6721	26604-26608	JJ	denotes	free
T6723	26609-26614	JJ	denotes	fatty
T6722	26615-26619	NN	denotes	acid
T6724	26619-26621	,	denotes	,
T6725	26621-26628	JJ	denotes	organic
T6726	26629-26633	NN	denotes	acid
T6727	26633-26635	,	denotes	,
T6728	26635-26638	CC	denotes	and
T6729	26639-26648	NN	denotes	carnitine
T6717	26649-26660	NNS	denotes	experiments
T6730	26660-26661	-RRB-	denotes	)
T6731	26662-26667	JJ	denotes	prior
T6732	26668-26670	IN	denotes	to
T6733	26671-26679	NN	denotes	analysis
T6734	26679-26680	.	denotes	.
T6735	26680-26840	sentence	denotes	Glucose concentration was measured in 10 μl sera using an Ektachem DT II system (Johnson and Johnson Clinical Diagnostics, Rochester, New York, United States).
T6736	26681-26688	NN	denotes	Glucose
T6737	26689-26702	NN	denotes	concentration
T6739	26703-26706	VBD	denotes	was
T6738	26707-26715	VBN	denotes	measured
T6740	26716-26718	IN	denotes	in
T6741	26719-26721	CD	denotes	10
T6742	26722-26724	NN	denotes	μl
T6743	26725-26729	NNS	denotes	sera
T6744	26730-26735	VBG	denotes	using
T6745	26736-26738	DT	denotes	an
T6747	26739-26747	NN	denotes	Ektachem
T6748	26748-26750	NN	denotes	DT
T6749	26751-26753	CD	denotes	II
T6746	26754-26760	NN	denotes	system
T6750	26761-26762	-LRB-	denotes	(
T6752	26762-26769	NNP	denotes	Johnson
T6753	26770-26773	CC	denotes	and
T6754	26774-26781	NNP	denotes	Johnson
T6755	26782-26790	NNP	denotes	Clinical
T6751	26791-26802	NNP	denotes	Diagnostics
T6756	26802-26804	,	denotes	,
T6757	26804-26813	NNP	denotes	Rochester
T6758	26813-26815	,	denotes	,
T6759	26815-26818	NNP	denotes	New
T6760	26819-26823	NNP	denotes	York
T6761	26823-26825	,	denotes	,
T6762	26825-26831	NNP	denotes	United
T6763	26832-26838	NNP	denotes	States
T6764	26838-26839	-RRB-	denotes	)
T6765	26839-26840	.	denotes	.
T6766	26840-27005	sentence	denotes	Total non-esterified fatty acids (NEFA) were measured by an enzymatic, colorimetric method (“NEFA-C” reagents, Wako Diagnostics, Richmond, Virginia, United States).
T6767	26841-26846	JJ	denotes	Total
T6769	26847-26861	JJ	denotes	non-esterified
T6770	26862-26867	JJ	denotes	fatty
T6768	26868-26873	NNS	denotes	acids
T6772	26874-26875	-LRB-	denotes	(
T6773	26875-26879	NN	denotes	NEFA
T6774	26879-26880	-RRB-	denotes	)
T6775	26881-26885	VBD	denotes	were
T6771	26886-26894	VBN	denotes	measured
T6776	26895-26897	IN	denotes	by
T6777	26898-26900	DT	denotes	an
T6779	26901-26910	JJ	denotes	enzymatic
T6780	26910-26912	,	denotes	,
T6781	26912-26924	JJ	denotes	colorimetric
T6778	26925-26931	NN	denotes	method
T6782	26932-26933	-LRB-	denotes	(
T6784	26933-26934	``	denotes	“
T6785	26934-26938	NN	denotes	NEFA
T6787	26938-26939	HYPH	denotes	-
T6786	26939-26940	NN	denotes	C
T6789	26940-26941	''	denotes	”
T6788	26942-26950	NNS	denotes	reagents
T6790	26950-26952	,	denotes	,
T6791	26952-26956	NNP	denotes	Wako
T6783	26957-26968	NNP	denotes	Diagnostics
T6792	26968-26970	,	denotes	,
T6793	26970-26978	NNP	denotes	Richmond
T6794	26978-26980	,	denotes	,
T6795	26980-26988	NNP	denotes	Virginia
T6796	26988-26990	,	denotes	,
T6797	26990-26996	NNP	denotes	United
T6798	26997-27003	NNP	denotes	States
T6799	27003-27004	-RRB-	denotes	)
T6800	27004-27005	.	denotes	.
T6801	27005-27150	sentence	denotes	The assay was modified to accommodate a reduced sample size (10 μl) and use of a microplate reader for measurement of optical density at 550 nm.
T6802	27006-27009	DT	denotes	The
T6803	27010-27015	NN	denotes	assay
T6805	27016-27019	VBD	denotes	was
T6804	27020-27028	VBN	denotes	modified
T6806	27029-27031	TO	denotes	to
T6807	27032-27043	VB	denotes	accommodate
T6808	27044-27045	DT	denotes	a
T6810	27046-27053	VBN	denotes	reduced
T6811	27054-27060	NN	denotes	sample
T6809	27061-27065	NN	denotes	size
T6812	27066-27067	-LRB-	denotes	(
T6814	27067-27069	CD	denotes	10
T6813	27070-27072	NN	denotes	μl
T6815	27072-27073	-RRB-	denotes	)
T6816	27074-27077	CC	denotes	and
T6817	27078-27081	NN	denotes	use
T6818	27082-27084	IN	denotes	of
T6819	27085-27086	DT	denotes	a
T6821	27087-27097	NN	denotes	microplate
T6820	27098-27104	NN	denotes	reader
T6822	27105-27108	IN	denotes	for
T6823	27109-27120	NN	denotes	measurement
T6824	27121-27123	IN	denotes	of
T6825	27124-27131	JJ	denotes	optical
T6826	27132-27139	NN	denotes	density
T6827	27140-27142	IN	denotes	at
T6828	27143-27146	CD	denotes	550
T6829	27147-27149	NN	denotes	nm
T6830	27149-27150	.	denotes	.
T6831	27150-27472	sentence	denotes	Urine organic acid analyses were performed using gas chromatography-mass spectroscopy as previously described [22,30], except tetracosane (C24; Sigma, St. Louis, Missouri, United States) was used as the internal standard, and quantitative determinations were made based on comparison with synthetic calibration standards.
T6832	27151-27156	NN	denotes	Urine
T6834	27157-27164	JJ	denotes	organic
T6833	27165-27169	NN	denotes	acid
T6835	27170-27178	NNS	denotes	analyses
T6837	27179-27183	VBD	denotes	were
T6836	27184-27193	VBN	denotes	performed
T6838	27194-27199	VBG	denotes	using
T6839	27200-27203	NN	denotes	gas
T6840	27204-27218	NN	denotes	chromatography
T6841	27218-27219	HYPH	denotes	-
T6842	27219-27223	NN	denotes	mass
T6843	27224-27236	NN	denotes	spectroscopy
T6844	27237-27239	IN	denotes	as
T6846	27240-27250	RB	denotes	previously
T6845	27251-27260	VBN	denotes	described
T6847	27261-27262	-LRB-	denotes	[
T6849	27262-27264	CD	denotes	22
T6850	27264-27265	,	denotes	,
T6848	27265-27267	CD	denotes	30
T6851	27267-27268	-RRB-	denotes	]
T6852	27268-27270	,	denotes	,
T6853	27270-27276	IN	denotes	except
T6854	27277-27288	NN	denotes	tetracosane
T6856	27289-27290	-LRB-	denotes	(
T6858	27290-27293	NN	denotes	C24
T6859	27293-27294	:	denotes	;
T6857	27295-27300	NNP	denotes	Sigma
T6860	27300-27302	,	denotes	,
T6861	27302-27305	NNP	denotes	St.
T6862	27306-27311	NNP	denotes	Louis
T6863	27311-27313	,	denotes	,
T6864	27313-27321	NNP	denotes	Missouri
T6865	27321-27323	,	denotes	,
T6866	27323-27329	NNP	denotes	United
T6867	27330-27336	NNP	denotes	States
T6868	27336-27337	-RRB-	denotes	)
T6869	27338-27341	VBD	denotes	was
T6855	27342-27346	VBN	denotes	used
T6870	27347-27349	IN	denotes	as
T6871	27350-27353	DT	denotes	the
T6873	27354-27362	JJ	denotes	internal
T6872	27363-27371	NN	denotes	standard
T6874	27371-27373	,	denotes	,
T6875	27373-27376	CC	denotes	and
T6876	27377-27389	JJ	denotes	quantitative
T6877	27390-27404	NNS	denotes	determinations
T6879	27405-27409	VBD	denotes	were
T6878	27410-27414	VBN	denotes	made
T6880	27415-27420	VBN	denotes	based
T6881	27421-27423	IN	denotes	on
T6882	27424-27434	NN	denotes	comparison
T6883	27435-27439	IN	denotes	with
T6884	27440-27449	JJ	denotes	synthetic
T6886	27450-27461	NN	denotes	calibration
T6885	27462-27471	NNS	denotes	standards
T6887	27471-27472	.	denotes	.
T6888	27472-27581	sentence	denotes	Acylcarnitine analyses in serum and bile were conducted using electrospray tandem mass spectrometry [31,32].
T6889	27473-27486	NN	denotes	Acylcarnitine
T6890	27487-27495	NNS	denotes	analyses
T6892	27496-27498	IN	denotes	in
T6893	27499-27504	NN	denotes	serum
T6894	27505-27508	CC	denotes	and
T6895	27509-27513	NN	denotes	bile
T6896	27514-27518	VBD	denotes	were
T6891	27519-27528	VBN	denotes	conducted
T6897	27529-27534	VBG	denotes	using
T6898	27535-27547	NN	denotes	electrospray
T6900	27548-27554	JJ	denotes	tandem
T6901	27555-27559	NN	denotes	mass
T6899	27560-27572	NN	denotes	spectrometry
T6902	27573-27574	-LRB-	denotes	[
T6904	27574-27576	CD	denotes	31
T6905	27576-27577	,	denotes	,
T6903	27577-27579	CD	denotes	32
T6906	27579-27580	-RRB-	denotes	]
T6907	27580-27581	.	denotes	.
T6957	27583-27597	NN	denotes	Histopathology
T6958	27597-27598	.	denotes	.
T6959	27598-27869	sentence	denotes	Twelve mice were examined for gross and histologic abnormalities, including one male and one female MCAD−/− mouse 18-mo-old, one male and one female MCAD+/+ mouse 6-mo-old, two male and two female MCAD−/− mice 4-wk-old, and two male and two female MCAD+/+ mice 4-wk-old.
T6960	27599-27605	CD	denotes	Twelve
T6961	27606-27610	NNS	denotes	mice
T6963	27611-27615	VBD	denotes	were
T6962	27616-27624	VBN	denotes	examined
T6964	27625-27628	IN	denotes	for
T6965	27629-27634	JJ	denotes	gross
T6967	27635-27638	CC	denotes	and
T6968	27639-27649	JJ	denotes	histologic
T6966	27650-27663	NNS	denotes	abnormalities
T6969	27663-27665	,	denotes	,
T6970	27665-27674	VBG	denotes	including
T6971	27675-27678	CD	denotes	one
T6972	27679-27683	JJ	denotes	male
T6973	27684-27687	CC	denotes	and
T6974	27688-27691	CD	denotes	one
T6976	27692-27698	JJ	denotes	female
T6977	27699-27703	NN	denotes	MCAD
T6978	27703-27704	SYM	denotes	−
T6979	27704-27705	HYPH	denotes	/
T6980	27705-27706	SYM	denotes	−
T6975	27707-27712	NN	denotes	mouse
T6981	27713-27715	CD	denotes	18
T6983	27715-27716	HYPH	denotes	-
T6982	27716-27718	NN	denotes	mo
T6985	27718-27719	HYPH	denotes	-
T6984	27719-27722	JJ	denotes	old
T6986	27722-27724	,	denotes	,
T6987	27724-27727	CD	denotes	one
T6988	27728-27732	JJ	denotes	male
T6989	27733-27736	CC	denotes	and
T6990	27737-27740	CD	denotes	one
T6992	27741-27747	JJ	denotes	female
T6993	27748-27752	NN	denotes	MCAD
T6994	27752-27753	SYM	denotes	+
T6995	27753-27754	HYPH	denotes	/
T6996	27754-27755	SYM	denotes	+
T6991	27756-27761	NN	denotes	mouse
T6997	27762-27763	CD	denotes	6
T6999	27763-27764	HYPH	denotes	-
T6998	27764-27766	NN	denotes	mo
T7001	27766-27767	HYPH	denotes	-
T7000	27767-27770	JJ	denotes	old
T7002	27770-27772	,	denotes	,
T7003	27772-27775	CD	denotes	two
T7004	27776-27780	JJ	denotes	male
T7005	27781-27784	CC	denotes	and
T7006	27785-27788	CD	denotes	two
T7008	27789-27795	JJ	denotes	female
T7009	27796-27800	NN	denotes	MCAD
T7010	27800-27801	SYM	denotes	−
T7011	27801-27802	HYPH	denotes	/
T7012	27802-27803	SYM	denotes	−
T7007	27804-27808	NNS	denotes	mice
T7013	27809-27810	CD	denotes	4
T7015	27810-27811	HYPH	denotes	-
T7014	27811-27813	NN	denotes	wk
T7017	27813-27814	HYPH	denotes	-
T7016	27814-27817	JJ	denotes	old
T7018	27817-27819	,	denotes	,
T7019	27819-27822	CC	denotes	and
T7020	27823-27826	CD	denotes	two
T7021	27827-27831	JJ	denotes	male
T7022	27832-27835	CC	denotes	and
T7023	27836-27839	CD	denotes	two
T7025	27840-27846	JJ	denotes	female
T7026	27847-27851	NN	denotes	MCAD
T7027	27851-27852	SYM	denotes	+
T7028	27852-27853	HYPH	denotes	/
T7029	27853-27854	SYM	denotes	+
T7024	27855-27859	NNS	denotes	mice
T7030	27860-27861	CD	denotes	4
T7032	27861-27862	HYPH	denotes	-
T7031	27862-27864	NN	denotes	wk
T7034	27864-27865	HYPH	denotes	-
T7033	27865-27868	JJ	denotes	old
T7035	27868-27869	.	denotes	.
T7036	27869-28110	sentence	denotes	Kidney, spleen, pancreas, liver, brain, heart, testicles, ovaries, and skeletal muscle were fixed by immersion in buffered 10% formalin, processed routinely for paraffin sectioning, sectioned at 5 μm, and stained with hematoxylin and eosin.
T7037	27870-27876	NN	denotes	Kidney
T7039	27876-27878	,	denotes	,
T7040	27878-27884	NN	denotes	spleen
T7041	27884-27886	,	denotes	,
T7042	27886-27894	NN	denotes	pancreas
T7043	27894-27896	,	denotes	,
T7044	27896-27901	NN	denotes	liver
T7045	27901-27903	,	denotes	,
T7046	27903-27908	NN	denotes	brain
T7047	27908-27910	,	denotes	,
T7048	27910-27915	NN	denotes	heart
T7049	27915-27917	,	denotes	,
T7050	27917-27926	NNS	denotes	testicles
T7051	27926-27928	,	denotes	,
T7052	27928-27935	NNS	denotes	ovaries
T7053	27935-27937	,	denotes	,
T7054	27937-27940	CC	denotes	and
T7055	27941-27949	JJ	denotes	skeletal
T7056	27950-27956	NN	denotes	muscle
T7057	27957-27961	VBD	denotes	were
T7038	27962-27967	VBN	denotes	fixed
T7058	27968-27970	IN	denotes	by
T7059	27971-27980	NN	denotes	immersion
T7060	27981-27983	IN	denotes	in
T7061	27984-27992	VBN	denotes	buffered
T7063	27993-27995	CD	denotes	10
T7064	27995-27996	NN	denotes	%
T7062	27997-28005	NN	denotes	formalin
T7065	28005-28007	,	denotes	,
T7066	28007-28016	VBN	denotes	processed
T7067	28017-28026	RB	denotes	routinely
T7068	28027-28030	IN	denotes	for
T7069	28031-28039	NN	denotes	paraffin
T7070	28040-28050	NN	denotes	sectioning
T7071	28050-28052	,	denotes	,
T7072	28052-28061	VBN	denotes	sectioned
T7073	28062-28064	IN	denotes	at
T7074	28065-28066	CD	denotes	5
T7075	28067-28069	NN	denotes	μm
T7076	28069-28071	,	denotes	,
T7077	28071-28074	CC	denotes	and
T7078	28075-28082	VBN	denotes	stained
T7079	28083-28087	IN	denotes	with
T7080	28088-28099	NN	denotes	hematoxylin
T7081	28100-28103	CC	denotes	and
T7082	28104-28109	NN	denotes	eosin
T7083	28109-28110	.	denotes	.
T7084	28110-28211	sentence	denotes	Frozen liver sections were prepared using standard methods and sections were stained with Oil-red-O.
T7085	28111-28117	VBN	denotes	Frozen
T7087	28118-28123	NN	denotes	liver
T7086	28124-28132	NNS	denotes	sections
T7089	28133-28137	VBD	denotes	were
T7088	28138-28146	VBN	denotes	prepared
T7090	28147-28152	VBG	denotes	using
T7091	28153-28161	JJ	denotes	standard
T7092	28162-28169	NNS	denotes	methods
T7093	28170-28173	CC	denotes	and
T7094	28174-28182	NNS	denotes	sections
T7096	28183-28187	VBD	denotes	were
T7095	28188-28195	VBN	denotes	stained
T7097	28196-28200	IN	denotes	with
T7098	28201-28204	NN	denotes	Oil
T7100	28204-28205	HYPH	denotes	-
T7101	28205-28208	JJ	denotes	red
T7102	28208-28209	HYPH	denotes	-
T7099	28209-28210	NN	denotes	O
T7103	28210-28211	.	denotes	.
T7104	28211-28270	sentence	denotes	Slides were examined without knowledge of genotype or age.
T7105	28212-28218	NNS	denotes	Slides
T7107	28219-28223	VBD	denotes	were
T7106	28224-28232	VBN	denotes	examined
T7108	28233-28240	IN	denotes	without
T7109	28241-28250	NN	denotes	knowledge
T7110	28251-28253	IN	denotes	of
T7111	28254-28262	NN	denotes	genotype
T7112	28263-28265	CC	denotes	or
T7113	28266-28269	NN	denotes	age
T7114	28269-28270	.	denotes	.
T7115	28270-28333	sentence	denotes	Other mice were examined because of sporadic clinical disease.
T7116	28271-28276	JJ	denotes	Other
T7117	28277-28281	NNS	denotes	mice
T7119	28282-28286	VBD	denotes	were
T7118	28287-28295	VBN	denotes	examined
T7120	28296-28303	IN	denotes	because
T7121	28304-28306	IN	denotes	of
T7122	28307-28315	JJ	denotes	sporadic
T7124	28316-28324	JJ	denotes	clinical
T7123	28325-28332	NN	denotes	disease
T7125	28332-28333	.	denotes	.
T7126	28333-28408	sentence	denotes	Those with visible cardiac enlargement were evaluated for cardiac lesions.
T7127	28334-28339	DT	denotes	Those
T7129	28340-28344	IN	denotes	with
T7130	28345-28352	JJ	denotes	visible
T7132	28353-28360	JJ	denotes	cardiac
T7131	28361-28372	NN	denotes	enlargement
T7133	28373-28377	VBD	denotes	were
T7128	28378-28387	VBN	denotes	evaluated
T7134	28388-28391	IN	denotes	for
T7135	28392-28399	JJ	denotes	cardiac
T7136	28400-28407	NNS	denotes	lesions
T7137	28407-28408	.	denotes	.
T7201	28410-28421	JJ	denotes	Statistical
T7202	28422-28430	NNS	denotes	analyses
T7203	28430-28431	.	denotes	.
T7204	28431-28519	sentence	denotes	Results between groups were tested for statistical significance using Student's t-test.
T7205	28432-28439	NNS	denotes	Results
T7207	28440-28447	IN	denotes	between
T7208	28448-28454	NNS	denotes	groups
T7209	28455-28459	VBD	denotes	were
T7206	28460-28466	VBN	denotes	tested
T7210	28467-28470	IN	denotes	for
T7211	28471-28482	JJ	denotes	statistical
T7212	28483-28495	NN	denotes	significance
T7213	28496-28501	VBG	denotes	using
T7214	28502-28509	NNP	denotes	Student
T7216	28509-28511	POS	denotes	's
T7217	28512-28513	NN	denotes	t
T7218	28513-28514	HYPH	denotes	-
T7215	28514-28518	NN	denotes	test
T7219	28518-28519	.	denotes	.
T7220	28519-28554	sentence	denotes	A p < 0.05 was set as significant.
T7221	28520-28521	DT	denotes	A
T7222	28522-28523	NN	denotes	p
T7224	28524-28525	SYM	denotes	<
T7225	28526-28530	CD	denotes	0.05
T7226	28531-28534	VBD	denotes	was
T7223	28535-28538	VBN	denotes	set
T7227	28539-28541	IN	denotes	as
T7228	28542-28553	JJ	denotes	significant
T7229	28553-28554	.	denotes	.
R1	T78	T79	amod	fatty,acid
R10	T88	T87	advmod	better,understand
R100	T215	T213	pobj	analysis,for
R1000	T1418	T1414	punct	−,MCAD
R1001	T1419	T1413	auxpass	were,ascertained
R1002	T1420	T1413	prep	by,ascertained
R1003	T1421	T1422	compound	immunoblot,analysis
R1004	T1422	T1420	pobj	analysis,by
R1005	T1423	T1422	prep	of,analysis
R1006	T1424	T1423	pobj	offspring,of
R1007	T1425	T1422	prep	with,analysis
R1008	T1426	T1427	amod	subsequent,perpetuation
R1009	T1427	T1425	pobj	perpetuation,with
R101	T216	T215	prep	of,analysis
R1010	T1428	T1427	prep	of,perpetuation
R1011	T1429	T1430	nmod	MCAD,mice
R1012	T1430	T1428	pobj	mice,of
R1013	T1431	T1429	punct	−,MCAD
R1014	T1432	T1429	punct	/,MCAD
R1015	T1433	T1429	punct	−,MCAD
R1016	T1434	T1429	cc	and,MCAD
R1017	T1435	T1429	conj	MCAD,MCAD
R1018	T1436	T1435	punct	+,MCAD
R1019	T1437	T1435	punct	/,MCAD
R102	T217	T218	det	the,roles
R1020	T1438	T1435	punct	+,MCAD
R1021	T1439	T1427	prep	as,perpetuation
R1022	T1440	T1441	amod	separate,groups
R1023	T1441	T1439	pobj	groups,as
R1024	T1442	T1413	punct	.,ascertained
R1025	T1606	T1607	compound	RNA,Analysis
R1026	T1609	T1610	compound	RT,PCR
R1027	T1610	T1612	compound	PCR,amplification
R1028	T1611	T1610	punct	-,PCR
R1029	T1612	T1613	nsubj	amplification,amplified
R103	T218	T216	pobj	roles,of
R1030	T1614	T1612	prep	from,amplification
R1031	T1615	T1616	nmod	exon,7
R1032	T1616	T1614	pobj	7,from
R1033	T1617	T1616	prep	to,7
R1034	T1618	T1617	pobj	11,to
R1035	T1619	T1612	prep	from,amplification
R1036	T1620	T1621	amod	total,RNA
R1037	T1621	T1619	pobj	RNA,from
R1038	T1622	T1621	compound	heart,RNA
R1039	T1623	T1624	det	the,fragment
R104	T219	T218	prep	of,roles
R1040	T1624	T1613	dobj	fragment,amplified
R1041	T1625	T1624	amod	expected,fragment
R1042	T1626	T1627	nummod	600,pair
R1043	T1627	T1624	nmod	pair,fragment
R1044	T1628	T1627	punct	-,pair
R1045	T1629	T1627	nmod	base,pair
R1046	T1630	T1627	punct	(,pair
R1047	T1631	T1627	appos	bp,pair
R1048	T1632	T1624	punct	),fragment
R1049	T1633	T1624	prep	in,fragment
R105	T220	T221	amod	fatty,acid
R1050	T1634	T1635	nmod	MCAD,mice
R1051	T1635	T1633	pobj	mice,in
R1052	T1636	T1634	punct	+,MCAD
R1053	T1637	T1634	punct	/,MCAD
R1054	T1638	T1634	punct	+,MCAD
R1055	T1639	T1634	cc	and,MCAD
R1056	T1640	T1634	conj	MCAD,MCAD
R1057	T1641	T1640	punct	+,MCAD
R1058	T1642	T1640	punct	/,MCAD
R1059	T1643	T1640	punct	−,MCAD
R106	T221	T222	compound	acid,oxidation
R1060	T1644	T1624	punct	", ",fragment
R1061	T1645	T1624	cc	and,fragment
R1062	T1646	T1647	det	a,fragment
R1063	T1647	T1624	conj	fragment,fragment
R1064	T1648	T1649	nummod	1.5,kb
R1065	T1649	T1647	compound	kb,fragment
R1066	T1650	T1649	punct	-,kb
R1067	T1651	T1647	prep	in,fragment
R1068	T1652	T1653	nmod	MCAD,mice
R1069	T1653	T1651	pobj	mice,in
R107	T222	T219	pobj	oxidation,of
R1070	T1654	T1652	punct	−,MCAD
R1071	T1655	T1652	punct	/,MCAD
R1072	T1656	T1652	punct	−,MCAD
R1073	T1657	T1658	punct	(,shown
R1074	T1658	T1613	parataxis	shown,amplified
R1075	T1659	T1658	nsubj	data,shown
R1076	T1660	T1658	neg	not,shown
R1077	T1661	T1658	punct	),shown
R1078	T1662	T1613	punct	.,amplified
R1079	T1664	T1665	compound	Sequence,analysis
R108	T223	T218	cc	and,roles
R1080	T1665	T1666	nsubj	analysis,revealed
R1081	T1667	T1665	prep	of,analysis
R1082	T1668	T1669	det	the,fragment
R1083	T1669	T1667	pobj	fragment,of
R1084	T1670	T1671	nummod	1.5,kb
R1085	T1671	T1669	compound	kb,fragment
R1086	T1672	T1671	punct	-,kb
R1087	T1673	T1674	mark	that,consisted
R1088	T1674	T1666	ccomp	consisted,revealed
R1089	T1675	T1676	det	the,fragment
R109	T224	T218	conj	pathogenesis,roles
R1090	T1676	T1674	nsubj	fragment,consisted
R1091	T1677	T1676	amod	amplified,fragment
R1092	T1678	T1674	prep	of,consisted
R1093	T1679	T1678	pobj	exon,of
R1094	T1680	T1679	nummod	7,exon
R1095	T1681	T1679	prep	to,exon
R1096	T1682	T1681	pobj	exon,to
R1097	T1683	T1682	nummod	10,exon
R1098	T1684	T1674	prep	with,consisted
R1099	T1685	T1686	nummod	280,bp
R11	T90	T91	det	the,pathogenesis
R110	T225	T224	prep	of,pathogenesis
R1100	T1686	T1684	pobj	bp,with
R1101	T1687	T1686	prep	of,bp
R1102	T1688	T1689	compound	pGEM,sequence
R1103	T1689	T1687	pobj	sequence,of
R1104	T1690	T1689	compound	plasmid,sequence
R1105	T1691	T1689	acl	followed,sequence
R1106	T1692	T1691	agent	by,followed
R1107	T1693	T1694	nmod	exons,8
R1108	T1694	T1692	pobj	8,by
R1109	T1695	T1696	punct	–,11
R111	T226	T227	amod	human,diseases
R1110	T1696	T1694	prep	11,8
R1111	T1697	T1666	punct	.,revealed
R1112	T1699	T1700	nsubjpass	Some,deleted
R1113	T1701	T1699	prep	of,Some
R1114	T1702	T1703	det	the,sequences
R1115	T1703	T1701	pobj	sequences,of
R1116	T1704	T1703	compound	plasmid,sequences
R1117	T1705	T1699	punct	", ",Some
R1118	T1706	T1699	cc	along,Some
R1119	T1707	T1706	dep	with,along
R112	T227	T225	pobj	diseases,of
R1120	T1708	T1709	det	the,sequence
R1121	T1709	T1699	conj	sequence,Some
R1122	T1710	T1709	compound	pPGKNEOpA,sequence
R1123	T1711	T1700	punct	", ",deleted
R1124	T1712	T1700	auxpass	were,deleted
R1125	T1713	T1700	prep	from,deleted
R1126	T1714	T1715	det	this,mRNA
R1127	T1715	T1713	pobj	mRNA,from
R1128	T1716	T1715	amod	spliced,mRNA
R1129	T1717	T1700	punct	.,deleted
R113	T228	T227	acl	involving,diseases
R1130	T1719	T1720	compound	Northern,blot
R1131	T1720	T1721	compound	blot,analysis
R1132	T1721	T1722	nsubj	analysis,revealed
R1133	T1723	T1724	nsubjpass	Acadm,expressed
R1134	T1724	T1722	advcl	expressed,revealed
R1135	T1725	T1724	auxpass	was,expressed
R1136	T1726	T1724	advmod	normally,expressed
R1137	T1727	T1724	prep	in,expressed
R1138	T1728	T1729	det	all,tissues
R1139	T1729	T1727	pobj	tissues,in
R114	T229	T230	amod	fatty,acid
R1140	T1730	T1729	acl	analyzed,tissues
R1141	T1731	T1729	prep	from,tissues
R1142	T1732	T1733	nmod	MCAD,mice
R1143	T1733	T1731	pobj	mice,from
R1144	T1734	T1732	punct	+,MCAD
R1145	T1735	T1732	punct	/,MCAD
R1146	T1736	T1732	punct	+,MCAD
R1147	T1737	T1724	prep	with,expressed
R1148	T1738	T1739	det	the,expression
R1149	T1739	T1742	nsubj	expression,occurring
R115	T230	T231	compound	acid,oxidation
R1150	T1740	T1741	advmod	most,robust
R1151	T1741	T1739	amod	robust,expression
R1152	T1742	T1737	pcomp	occurring,with
R1153	T1743	T1742	prep	in,occurring
R1154	T1744	T1745	amod	brown,fat
R1155	T1745	T1743	pobj	fat,in
R1156	T1746	T1745	punct	", ",fat
R1157	T1747	T1745	conj	kidney,fat
R1158	T1748	T1747	punct	", ",kidney
R1159	T1749	T1747	conj	heart,kidney
R116	T231	T228	dobj	oxidation,involving
R1160	T1750	T1749	punct	", ",heart
R1161	T1751	T1752	amod	skeletal,muscle
R1162	T1752	T1749	conj	muscle,heart
R1163	T1753	T1752	punct	", ",muscle
R1164	T1754	T1752	cc	and,muscle
R1165	T1755	T1752	conj	liver,muscle
R1166	T1756	T1724	prep	with,expressed
R1167	T1757	T1758	amod	minimal,expression
R1168	T1758	T1756	pobj	expression,with
R1169	T1759	T1758	prep	in,expression
R117	T232	T201	punct	.,reproduced
R1170	T1760	T1761	det	the,brain
R1171	T1761	T1759	pobj	brain,in
R1172	T1762	T1761	punct	", ",brain
R1173	T1763	T1764	amod	white,fat
R1174	T1764	T1761	conj	fat,brain
R1175	T1765	T1764	punct	", ",fat
R1176	T1766	T1764	cc	and,fat
R1177	T1767	T1764	conj	testes,fat
R1178	T1768	T1769	punct	(,Figure
R1179	T1769	T1722	parataxis	Figure,revealed
R1180	T1770	T1769	nummod	2,Figure
R1181	T1771	T1769	punct	),Figure
R1182	T1772	T1722	punct	.,revealed
R1183	T1774	T1775	advmod	Interestingly,detected
R1184	T1776	T1775	punct	", ",detected
R1185	T1777	T1778	mark	although,amplified
R1186	T1778	T1775	advcl	amplified,detected
R1187	T1779	T1780	compound	RT,PCR
R1188	T1780	T1778	nsubj	PCR,amplified
R1189	T1781	T1780	punct	-,PCR
R1190	T1782	T1783	det	an,transcript
R1191	T1783	T1778	dobj	transcript,amplified
R1192	T1784	T1785	advmod	incorrectly,spliced
R1193	T1785	T1783	amod	spliced,transcript
R1194	T1786	T1783	compound	Acadm,transcript
R1195	T1787	T1775	punct	", ",detected
R1196	T1788	T1789	det	no,transcripts
R1197	T1789	T1775	nsubjpass	transcripts,detected
R1198	T1790	T1789	compound	Acadm,transcripts
R1199	T1791	T1775	auxpass	were,detected
R12	T91	T87	dobj	pathogenesis,understand
R1200	T1792	T1775	prep	by,detected
R1201	T1793	T1794	compound	northern,blot
R1202	T1794	T1795	compound	blot,analysis
R1203	T1795	T1792	pobj	analysis,by
R1204	T1796	T1775	prep	from,detected
R1205	T1797	T1798	nmod	MCAD,mice
R1206	T1798	T1796	pobj	mice,from
R1207	T1799	T1797	punct	−,MCAD
R1208	T1800	T1797	punct	/,MCAD
R1209	T1801	T1797	punct	−,MCAD
R1210	T1802	T1775	punct	.,detected
R1211	T1804	T1805	det	These,results
R1212	T1805	T1806	nsubj	results,suggest
R1213	T1807	T1806	advmod	strongly,suggest
R1214	T1808	T1809	mark	that,is
R1215	T1809	T1806	ccomp	is,suggest
R1216	T1810	T1811	det	the,RNA
R1217	T1811	T1809	nsubj	RNA,is
R1218	T1812	T1811	compound	mutant,RNA
R1219	T1813	T1809	acomp	unstable,is
R122	T43	T44	amod	Medium,Chain
R1220	T1814	T1809	cc	and,is
R1221	T1815	T1809	conj	degraded,is
R1222	T1816	T1815	advmod	rapidly,degraded
R1223	T1817	T1809	cc	or,is
R1224	T1818	T1809	punct	", ",is
R1225	T1819	T1820	advmod	alternatively,undergoes
R1226	T1820	T1809	conj	undergoes,is
R1227	T1821	T1820	punct	", ",undergoes
R1228	T1822	T1823	npadvmod	nonsense,mediated
R1229	T1823	T1824	amod	mediated,decay
R123	T44	T47	compound	Chain,Dehydrogenase
R1230	T1824	T1820	dobj	decay,undergoes
R1231	T1825	T1824	compound	RNA,decay
R1232	T1826	T1806	punct	.,suggest
R1235	T1979	T1980	compound	Liver,Enzyme
R1236	T1980	T1981	compound	Enzyme,Analyses
R1237	T1983	T1984	compound	Immunoblot,analyses
R1238	T1984	T1985	nsubj	analyses,demonstrated
R1239	T1986	T1984	prep	of,analyses
R124	T46	T44	punct	-,Chain
R1240	T1987	T1988	compound	liver,homogenates
R1241	T1988	T1986	pobj	homogenates,of
R1242	T1989	T1984	prep	with,analyses
R1243	T1990	T1991	amod	anti-MCAD,antisera
R1244	T1991	T1989	pobj	antisera,with
R1245	T1992	T1993	mark	that,was
R1246	T1993	T1985	ccomp	was,demonstrated
R1247	T1994	T1995	det	the,monomer
R1248	T1995	T1993	nsubj	monomer,was
R1249	T1996	T1997	nummod	42,kDa
R125	T47	T51	compound	Dehydrogenase,Deficiency
R1250	T1997	T1995	compound	kDa,monomer
R1251	T1998	T1995	compound	MCAD,monomer
R1252	T1999	T1993	acomp	present,was
R1253	T2000	T1993	prep	in,was
R1254	T2001	T2002	nmod	MCAD,mice
R1255	T2002	T2000	pobj	mice,in
R1256	T2003	T2001	punct	+,MCAD
R1257	T2004	T2001	punct	/,MCAD
R1258	T2005	T2001	punct	+,MCAD
R1259	T2006	T2000	punct	", ",in
R126	T48	T49	compound	Acyl,CoA
R1260	T2007	T2000	cc	but,in
R1261	T2008	T2007	neg	not,but
R1262	T2009	T2000	conj	in,in
R1263	T2010	T2011	nmod	MCAD,mice
R1264	T2011	T2009	pobj	mice,in
R1265	T2012	T2010	punct	−,MCAD
R1266	T2013	T2010	punct	/,MCAD
R1267	T2014	T2010	punct	−,MCAD
R1268	T2015	T2016	punct	(,Figure
R1269	T2016	T1985	parataxis	Figure,demonstrated
R127	T49	T47	compound	CoA,Dehydrogenase
R1270	T2017	T2016	nummod	3,Figure
R1271	T2018	T2016	punct	),Figure
R1272	T2019	T1985	punct	.,demonstrated
R1273	T2021	T2022	prep	As,revealed
R1274	T2023	T2024	det	a,analysis
R1275	T2024	T2021	pobj	analysis,As
R1276	T2025	T2024	compound	control,analysis
R1277	T2026	T2022	punct	", ",revealed
R1278	T2027	T2028	amod	anti–short,chain
R1279	T2028	T2030	nmod	chain,dehydrogenase
R128	T50	T49	punct	-,CoA
R1280	T2029	T2028	punct	-,chain
R1281	T2030	T2034	nmod	dehydrogenase,SCAD
R1282	T2031	T2032	nmod	acyl,CoA
R1283	T2032	T2030	nmod	CoA,dehydrogenase
R1284	T2033	T2032	punct	-,CoA
R1285	T2034	T2036	nmod	SCAD,antisera
R1286	T2035	T2034	punct	(,SCAD
R1287	T2036	T2022	nsubj	antisera,revealed
R1288	T2037	T2034	punct	),SCAD
R1289	T2038	T2039	det	no,differences
R129	T52	T51	prep	in,Deficiency
R1290	T2039	T2022	dobj	differences,revealed
R1291	T2040	T2039	prep	in,differences
R1292	T2041	T2040	pobj	levels,in
R1293	T2042	T2041	prep	of,levels
R1294	T2043	T2042	pobj	expression,of
R1295	T2044	T2043	prep	of,expression
R1296	T2045	T2046	compound	SCAD,protein
R1297	T2046	T2044	pobj	protein,of
R1298	T2047	T2039	prep	between,differences
R1299	T2048	T2049	nmod	MCAD,mice
R13	T92	T91	prep	of,pathogenesis
R130	T53	T54	npadvmod	Gene,Targeted
R1300	T2049	T2047	pobj	mice,between
R1301	T2050	T2048	punct	+,MCAD
R1302	T2051	T2048	punct	/,MCAD
R1303	T2052	T2048	punct	+,MCAD
R1304	T2053	T2048	cc	and,MCAD
R1305	T2054	T2048	conj	MCAD,MCAD
R1306	T2055	T2054	punct	−,MCAD
R1307	T2056	T2054	punct	/,MCAD
R1308	T2057	T2054	punct	−,MCAD
R1309	T2058	T2059	punct	(,Figure
R131	T54	T56	amod	Targeted,Mice
R1310	T2059	T2022	parataxis	Figure,revealed
R1311	T2060	T2059	nummod	3,Figure
R1312	T2061	T2059	punct	),Figure
R1313	T2062	T2022	punct	.,revealed
R1314	T2064	T2065	compound	Enzyme,activity
R1315	T2065	T2066	nsubjpass	activity,analyzed
R1316	T2067	T2066	auxpass	was,analyzed
R1317	T2068	T2066	prep	in,analyzed
R1318	T2069	T2070	compound	mouse,homogenates
R1319	T2070	T2068	pobj	homogenates,in
R132	T55	T54	punct	-,Targeted
R1320	T2071	T2070	compound	liver,homogenates
R1321	T2072	T2066	advcl	using,analyzed
R1322	T2073	T2074	det	the,assay
R1323	T2074	T2072	dobj	assay,using
R1324	T2075	T2076	nmod	electron,flavoprotein
R1325	T2076	T2074	nmod	flavoprotein,assay
R1326	T2077	T2076	nmod	transport,flavoprotein
R1327	T2078	T2076	punct	(,flavoprotein
R1328	T2079	T2076	appos	ETF,flavoprotein
R1329	T2080	T2074	punct	),assay
R133	T56	T52	pobj	Mice,in
R1330	T2081	T2074	compound	reduction,assay
R1331	T2082	T2072	prep	with,using
R1332	T2083	T2084	compound	octanoyl,CoA
R1333	T2084	T2082	pobj	CoA,with
R1334	T2085	T2084	punct	-,CoA
R1335	T2086	T2087	punct	(,C8
R1336	T2087	T2084	parataxis	C8,CoA
R1337	T2088	T2087	punct	:,C8
R1338	T2089	T2087	nummod	0,C8
R1339	T2090	T2087	punct	),C8
R134	T58	T59	amod	"MCAD Gene Disruption in the Mouse Abstract Medium",chain
R1340	T2091	T2084	cc	and,CoA
R1341	T2092	T2093	compound	palmitoyl,CoA
R1342	T2093	T2084	conj	CoA,CoA
R1343	T2094	T2093	punct	-,CoA
R1344	T2095	T2096	punct	(,C16
R1345	T2096	T2093	parataxis	C16,CoA
R1346	T2097	T2096	punct	:,C16
R1347	T2098	T2096	nummod	0,C16
R1348	T2099	T2096	punct	),C16
R1349	T2100	T2084	prep	as,CoA
R135	T59	T61	nmod	chain,dehydrogenase
R1350	T2101	T2100	pobj	substrates,as
R1351	T2102	T2066	punct	.,analyzed
R1352	T2104	T2105	nmod	MCAD,mice
R1353	T2105	T2109	nsubj	mice,had
R1354	T2106	T2104	punct	−,MCAD
R1355	T2107	T2104	punct	/,MCAD
R1356	T2108	T2104	punct	−,MCAD
R1357	T2110	T2111	det	a,reduction
R1358	T2111	T2109	dobj	reduction,had
R1359	T2112	T2111	amod	significant,reduction
R136	T60	T59	punct	-,chain
R1360	T2113	T2111	prep	in,reduction
R1361	T2114	T2113	pobj	ability,in
R1362	T2115	T2116	aux	to,dehydrogenate
R1363	T2116	T2114	acl	dehydrogenate,ability
R1364	T2117	T2118	compound	octanoyl,CoA
R1365	T2118	T2116	dobj	CoA,dehydrogenate
R1366	T2119	T2118	punct	-,CoA
R1367	T2120	T2111	cc	and,reduction
R1368	T2121	T2122	det	a,reduction
R1369	T2122	T2111	conj	reduction,reduction
R137	T61	T65	nmod	dehydrogenase,deficiency
R1370	T2123	T2122	amod	modest,reduction
R1371	T2124	T2122	prep	in,reduction
R1372	T2125	T2124	pobj	activity,in
R1373	T2126	T2125	prep	toward,activity
R1374	T2127	T2128	compound	palmitoyl,CoA
R1375	T2128	T2126	pobj	CoA,toward
R1376	T2129	T2128	punct	-,CoA
R1377	T2130	T2109	prep	compared,had
R1378	T2131	T2130	prep	to,compared
R1379	T2132	T2133	nmod	MCAD,mice
R138	T62	T63	nmod	acyl,CoA
R1380	T2133	T2131	pobj	mice,to
R1381	T2134	T2132	punct	+,MCAD
R1382	T2135	T2132	punct	/,MCAD
R1383	T2136	T2132	punct	+,MCAD
R1384	T2137	T2138	punct	(,Table
R1385	T2138	T2109	parataxis	Table,had
R1386	T2139	T2138	nummod	1,Table
R1387	T2140	T2138	punct	),Table
R1388	T2141	T2109	punct	.,had
R1389	T2143	T2144	advmod	Specifically,reduced
R139	T63	T61	nmod	CoA,dehydrogenase
R1390	T2145	T2144	punct	", ",reduced
R1391	T2146	T2147	det	the,dehydrogenation
R1392	T2147	T2144	nsubjpass	dehydrogenation,reduced
R1393	T2148	T2147	prep	of,dehydrogenation
R1394	T2149	T2150	nmod	octanoyl,CoA
R1395	T2150	T2152	nmod	CoA,substrates
R1396	T2151	T2150	punct	-,CoA
R1397	T2152	T2148	pobj	substrates,of
R1398	T2153	T2150	cc	and,CoA
R1399	T2154	T2155	compound	palmitoyl,CoA
R14	T93	T94	det	this,disease
R140	T64	T63	punct	-,CoA
R1400	T2155	T2150	conj	CoA,CoA
R1401	T2156	T2155	punct	-,CoA
R1402	T2157	T2144	auxpass	were,reduced
R1403	T2158	T2144	prep	by,reduced
R1404	T2159	T2160	nummod	75,%
R1405	T2160	T2158	pobj	%,by
R1406	T2161	T2158	cc	and,by
R1407	T2162	T2158	conj	by,by
R1408	T2163	T2164	nummod	30,%
R1409	T2164	T2162	pobj	%,by
R141	T65	T69	nsubj	deficiency,is
R1410	T2165	T2144	punct	", ",reduced
R1411	T2166	T2144	advmod	respectively,reduced
R1412	T2167	T2144	punct	", ",reduced
R1413	T2168	T2144	prep	in,reduced
R1414	T2169	T2170	nmod	MCAD,mice
R1415	T2170	T2168	pobj	mice,in
R1416	T2171	T2169	punct	−,MCAD
R1417	T2172	T2169	punct	/,MCAD
R1418	T2173	T2169	punct	−,MCAD
R1419	T2174	T2175	mark	as,compared
R142	T66	T61	punct	(,dehydrogenase
R1420	T2175	T2144	advcl	compared,reduced
R1421	T2176	T2175	prep	to,compared
R1422	T2177	T2178	nmod	MCAD,controls
R1423	T2178	T2176	pobj	controls,to
R1424	T2179	T2177	punct	+,MCAD
R1425	T2180	T2177	punct	/,MCAD
R1426	T2181	T2177	punct	+,MCAD
R1427	T2182	T2144	punct	.,reduced
R1428	T2294	T2295	amod	Neonatal,Deaths
R1429	T2297	T2298	amod	Significant,mortality
R143	T67	T61	appos	MCAD,dehydrogenase
R1430	T2298	T2300	nsubjpass	mortality,noted
R1431	T2299	T2298	amod	neonatal,mortality
R1432	T2301	T2300	auxpass	was,noted
R1433	T2302	T2300	prep	in,noted
R1434	T2303	T2304	nmod	MCAD,pups
R1435	T2304	T2302	pobj	pups,in
R1436	T2305	T2303	punct	−,MCAD
R1437	T2306	T2303	punct	/,MCAD
R1438	T2307	T2303	punct	−,MCAD
R1439	T2308	T2300	punct	.,noted
R144	T68	T65	punct	),deficiency
R1440	T2310	T2311	mark	Although,born
R1441	T2311	T2317	advcl	born,survived
R1442	T2312	T2313	amod	equal,numbers
R1443	T2313	T2311	nsubjpass	numbers,born
R1444	T2314	T2313	prep	of,numbers
R1445	T2315	T2314	pobj	pups,of
R1446	T2316	T2311	auxpass	were,born
R1447	T2318	T2311	prep	from,born
R1448	T2319	T2320	nmod	MCAD,mice
R1449	T2320	T2318	pobj	mice,from
R145	T70	T71	det	the,disorder
R1450	T2321	T2319	punct	+,MCAD
R1451	T2322	T2319	punct	/,MCAD
R1452	T2323	T2319	punct	+,MCAD
R1453	T2324	T2319	cc	and,MCAD
R1454	T2325	T2319	conj	MCAD,MCAD
R1455	T2326	T2325	punct	−,MCAD
R1456	T2327	T2325	punct	/,MCAD
R1457	T2328	T2325	punct	−,MCAD
R1458	T2329	T2317	punct	", ",survived
R1459	T2330	T2331	advmod	only,%
R146	T71	T69	attr	disorder,is
R1460	T2331	T2317	nsubj	%,survived
R1461	T2332	T2331	nummod	41,%
R1462	T2333	T2331	prep	of,%
R1463	T2334	T2335	nmod	MCAD,mice
R1464	T2335	T2333	pobj	mice,of
R1465	T2336	T2334	punct	−,MCAD
R1466	T2337	T2334	punct	/,MCAD
R1467	T2338	T2334	punct	−,MCAD
R1468	T2339	T2317	prep	to,survived
R1469	T2340	T2339	pobj	weaning,to
R147	T72	T73	advmod	most,common
R1470	T2341	T2342	mark	as,compared
R1471	T2342	T2317	advcl	compared,survived
R1472	T2343	T2342	prep	to,compared
R1473	T2344	T2345	nummod	98,%
R1474	T2345	T2343	pobj	%,to
R1475	T2346	T2345	prep	of,%
R1476	T2347	T2348	nmod	MCAD,mice
R1477	T2348	T2346	pobj	mice,of
R1478	T2349	T2347	punct	+,MCAD
R1479	T2350	T2347	punct	/,MCAD
R148	T73	T71	amod	common,disorder
R1480	T2351	T2347	punct	+,MCAD
R1481	T2352	T2353	punct	(,Table
R1482	T2353	T2317	parataxis	Table,survived
R1483	T2354	T2353	nummod	1,Table
R1484	T2355	T2353	punct	),Table
R1485	T2356	T2317	punct	.,survived
R1486	T2358	T2359	det	The,mechanism
R1487	T2359	T2360	nsubj	mechanism,remains
R1488	T2361	T2359	prep	of,mechanism
R1489	T2362	T2363	amod	neonatal,loss
R149	T74	T71	amod	inherited,disorder
R1490	T2363	T2361	pobj	loss,of
R1491	T2364	T2360	acomp	undetermined,remains
R1492	T2365	T2360	punct	.,remains
R1493	T2367	T2368	det	The,pups
R1494	T2368	T2373	nsubjpass	pups,abandoned
R1495	T2369	T2368	nmod	MCAD,pups
R1496	T2370	T2369	punct	−,MCAD
R1497	T2371	T2369	punct	/,MCAD
R1498	T2372	T2369	punct	−,MCAD
R1499	T2374	T2373	auxpass	are,abandoned
R15	T94	T92	pobj	disease,of
R150	T75	T71	prep	of,disorder
R1500	T2375	T2376	advmod	more,frequently
R1501	T2376	T2373	advmod	frequently,abandoned
R1502	T2377	T2376	prep	than,frequently
R1503	T2378	T2379	nmod	MCAD,pups
R1504	T2379	T2377	pobj	pups,than
R1505	T2380	T2378	punct	+,MCAD
R1506	T2381	T2378	punct	/,MCAD
R1507	T2382	T2378	punct	+,MCAD
R1508	T2383	T2373	prep	for,abandoned
R1509	T2384	T2385	amod	unknown,reasons
R151	T76	T77	amod	mitochondrial,oxidation
R1510	T2385	T2383	pobj	reasons,for
R1511	T2386	T2373	punct	.,abandoned
R1512	T2388	T2389	nsubj	They,are
R1513	T2390	T2389	advmod	likely,are
R1514	T2391	T2392	advmod	more,prone
R1515	T2392	T2389	acomp	prone,are
R1516	T2393	T2392	prep	to,prone
R1517	T2394	T2393	pobj	hypothermia,to
R1518	T2395	T2392	prep	than,prone
R1519	T2396	T2395	pobj	MCAD,than
R152	T77	T75	pobj	oxidation,of
R1520	T2397	T2396	punct	+,MCAD
R1521	T2398	T2396	punct	/,MCAD
R1522	T2399	T2396	punct	+,MCAD
R1523	T2400	T2389	punct	.,are
R1524	T2402	T2403	prep	Because,compare
R1525	T2404	T2402	pcomp	of,Because
R1526	T2405	T2406	det	the,difficulty
R1527	T2406	T2402	pobj	difficulty,Because
R1528	T2407	T2406	prep	of,difficulty
R1529	T2408	T2407	pcomp	distinguishing,of
R153	T122	T127	nsubj	mice,developed
R1530	T2409	T2410	det	the,mutants
R1531	T2410	T2408	dobj	mutants,distinguishing
R1532	T2411	T2410	nmod	MCAD,mutants
R1533	T2412	T2411	punct	−,MCAD
R1534	T2413	T2411	punct	/,MCAD
R1535	T2414	T2411	punct	−,MCAD
R1536	T2415	T2408	prep	from,distinguishing
R1537	T2416	T2417	det	the,pups
R1538	T2417	T2415	pobj	pups,from
R1539	T2418	T2417	nmod	MCAD,pups
R154	T123	T122	nmod	MCAD,mice
R1540	T2419	T2418	punct	+,MCAD
R1541	T2420	T2418	punct	/,MCAD
R1542	T2421	T2418	punct	−,MCAD
R1543	T2422	T2417	amod	heterozygous,pups
R1544	T2423	T2408	prep	by,distinguishing
R1545	T2424	T2425	amod	molecular,analysis
R1546	T2425	T2423	pobj	analysis,by
R1547	T2426	T2406	prep	due,difficulty
R1548	T2427	T2426	pcomp	to,due
R1549	T2428	T2429	det	the,mutation
R155	T124	T123	punct	−,MCAD
R1550	T2429	T2426	pobj	mutation,due
R1551	T2430	T2429	compound	insertion,mutation
R1552	T2431	T2403	punct	", ",compare
R1553	T2432	T2403	nsubj	we,compare
R1554	T2433	T2403	aux	could,compare
R1555	T2434	T2403	advmod	only,compare
R1556	T2435	T2436	nmod	MCAD,matings
R1557	T2436	T2403	dobj	matings,compare
R1558	T2437	T2435	punct	+,MCAD
R1559	T2438	T2435	punct	/,MCAD
R156	T125	T123	punct	/,MCAD
R1560	T2439	T2435	punct	+,MCAD
R1561	T2440	T2435	punct	×,MCAD
R1562	T2441	T2435	appos	MCAD,MCAD
R1563	T2442	T2441	punct	+,MCAD
R1564	T2443	T2441	punct	/,MCAD
R1565	T2444	T2441	punct	+,MCAD
R1566	T2445	T2403	prep	with,compare
R1567	T2446	T2447	nmod	MCAD,matings
R1568	T2447	T2445	pobj	matings,with
R1569	T2448	T2446	punct	−,MCAD
R157	T126	T123	punct	−,MCAD
R1570	T2449	T2446	punct	/,MCAD
R1571	T2450	T2446	punct	−,MCAD
R1572	T2451	T2446	punct	×,MCAD
R1573	T2452	T2446	appos	MCAD,MCAD
R1574	T2453	T2452	punct	−,MCAD
R1575	T2454	T2452	punct	/,MCAD
R1576	T2455	T2452	punct	−,MCAD
R1577	T2456	T2403	punct	.,compare
R1578	T2458	T2459	advmod	Thus,were
R1579	T2460	T2459	punct	", ",were
R158	T128	T129	det	an,aciduria
R1580	T2461	T2459	nsubj	we,were
R1581	T2462	T2459	acomp	unable,were
R1582	T2463	T2464	aux	to,evaluate
R1583	T2464	T2462	xcomp	evaluate,unable
R1584	T2465	T2466	det	the,pedigrees
R1585	T2466	T2464	dobj	pedigrees,evaluate
R1586	T2467	T2464	prep	from,evaluate
R1587	T2468	T2469	amod	heterozygous,matings
R1588	T2469	T2467	pobj	matings,from
R1589	T2470	T2459	punct	.,were
R159	T129	T127	dobj	aciduria,developed
R1590	T2566	T2565	cc	and,Fasting
R1591	T2567	T2568	compound	Cold,Intolerance
R1592	T2568	T2565	conj	Intolerance,Fasting
R1593	T2570	T2571	prep	In,fasted
R1594	T2572	T2570	pobj	order,In
R1595	T2573	T2574	aux	to,examine
R1596	T2574	T2572	acl	examine,order
R1597	T2575	T2576	det	the,effects
R1598	T2576	T2574	dobj	effects,examine
R1599	T2577	T2576	compound	stress,effects
R16	T95	T89	punct	", ",developed
R160	T130	T129	amod	organic,aciduria
R1600	T2578	T2576	prep	of,effects
R1601	T2579	T2578	pobj	fasting,of
R1602	T2580	T2576	prep	on,effects
R1603	T2581	T2582	npadvmod	MCAD,deficient
R1604	T2582	T2584	amod	deficient,mice
R1605	T2583	T2582	punct	-,deficient
R1606	T2584	T2580	pobj	mice,on
R1607	T2585	T2571	punct	", ",fasted
R1608	T2586	T2571	nsubjpass	they,fasted
R1609	T2587	T2571	auxpass	were,fasted
R161	T131	T129	cc	and,aciduria
R1610	T2588	T2571	prep	for,fasted
R1611	T2589	T2590	nummod	24,h
R1612	T2590	T2588	pobj	h,for
R1613	T2591	T2592	amod	prior,to
R1614	T2592	T2571	prep	to,fasted
R1615	T2593	T2592	pobj	analysis,to
R1616	T2594	T2571	punct	.,fasted
R1617	T2596	T2597	nmod	MCAD,mice
R1618	T2597	T2601	nsubj	mice,displayed
R1619	T2598	T2596	punct	−,MCAD
R162	T132	T133	amod	fatty,liver
R1620	T2599	T2596	punct	/,MCAD
R1621	T2600	T2596	punct	−,MCAD
R1622	T2602	T2603	amod	lower,glucose
R1623	T2603	T2601	dobj	glucose,displayed
R1624	T2604	T2603	compound	serum,glucose
R1625	T2605	T2603	cc	and,glucose
R1626	T2606	T2607	amod	elevated,levels
R1627	T2607	T2603	conj	levels,glucose
R1628	T2608	T2609	npadvmod	serum,free
R1629	T2609	T2610	amod	free,acid
R163	T133	T129	conj	liver,aciduria
R1630	T2610	T2607	compound	acid,levels
R1631	T2611	T2610	amod	fatty,acid
R1632	T2612	T2613	mark	although,was
R1633	T2613	T2601	advcl	was,displayed
R1634	T2614	T2615	det	neither,result
R1635	T2615	T2613	nsubj	result,was
R1636	T2616	T2613	acomp	significant,was
R1637	T2617	T2613	punct	", ",was
R1638	T2618	T2619	mark	as,compared
R1639	T2619	T2613	advcl	compared,was
R164	T134	T127	punct	", ",developed
R1640	T2620	T2619	prep	to,compared
R1641	T2621	T2622	nmod	MCAD,mice
R1642	T2622	T2620	pobj	mice,to
R1643	T2623	T2621	punct	+,MCAD
R1644	T2624	T2621	punct	/,MCAD
R1645	T2625	T2621	punct	+,MCAD
R1646	T2626	T2627	punct	(,Table
R1647	T2627	T2601	parataxis	Table,displayed
R1648	T2628	T2627	nummod	1,Table
R1649	T2629	T2627	punct	),Table
R165	T135	T127	cc	and,developed
R1650	T2630	T2601	punct	.,displayed
R1651	T2632	T2633	aux	To,determine
R1652	T2633	T2634	advcl	determine,fasted
R1653	T2635	T2636	det	the,effects
R1654	T2636	T2633	dobj	effects,determine
R1655	T2637	T2636	prep	of,effects
R1656	T2638	T2639	compound	cold,stress
R1657	T2639	T2637	pobj	stress,of
R1658	T2640	T2634	punct	", ",fasted
R1659	T2641	T2634	nsubjpass	mice,fasted
R166	T136	T127	conj	showed,developed
R1660	T2642	T2634	auxpass	were,fasted
R1661	T2643	T2634	prep	for,fasted
R1662	T2644	T2645	nummod	18,h
R1663	T2645	T2643	pobj	h,for
R1664	T2646	T2634	cc	and,fasted
R1665	T2647	T2634	conj	placed,fasted
R1666	T2648	T2647	prep	in,placed
R1667	T2649	T2650	nummod	4,°C
R1668	T2650	T2651	compound	°C,environment
R1669	T2651	T2648	pobj	environment,in
R167	T137	T138	amod	profound,intolerance
R1670	T2652	T2647	prep	for,placed
R1671	T2653	T2654	det	a,period
R1672	T2654	T2652	pobj	period,for
R1673	T2655	T2656	nummod	3,h
R1674	T2656	T2654	compound	h,period
R1675	T2657	T2656	punct	-,h
R1676	T2658	T2634	punct	.,fasted
R1677	T2660	T2661	det	The,mice
R1678	T2661	T2666	nsubjpass	mice,compromised
R1679	T2662	T2661	nmod	MCAD,mice
R168	T138	T136	dobj	intolerance,showed
R1680	T2663	T2662	punct	−,MCAD
R1681	T2664	T2662	punct	/,MCAD
R1682	T2665	T2662	punct	−,MCAD
R1683	T2667	T2666	auxpass	were,compromised
R1684	T2668	T2666	advmod	significantly,compromised
R1685	T2669	T2666	prep	within,compromised
R1686	T2670	T2671	det	this,period
R1687	T2671	T2669	pobj	period,within
R1688	T2672	T2671	amod	short,period
R1689	T2673	T2671	prep	of,period
R169	T139	T138	compound	cold,intolerance
R1690	T2674	T2675	compound	cold,stress
R1691	T2675	T2673	pobj	stress,of
R1692	T2676	T2666	punct	", ",compromised
R1693	T2677	T2678	nsubj	some,severe
R1694	T2678	T2666	amod	severe,compromised
R1695	T2679	T2678	advmod	enough,severe
R1696	T2680	T2681	aux	to,result
R1697	T2681	T2678	xcomp	result,severe
R1698	T2682	T2681	prep	in,result
R1699	T2683	T2682	pobj	fatalities,in
R17	T96	T89	nsubj	we,developed
R170	T140	T136	prep	at,showed
R1700	T2684	T2666	punct	.,compromised
R1701	T2686	T2687	prep	After,was
R1702	T2688	T2689	nummod	1,h
R1703	T2689	T2686	pobj	h,After
R1704	T2690	T2689	prep	of,h
R1705	T2691	T2692	det	the,challenge
R1706	T2692	T2690	pobj	challenge,of
R1707	T2693	T2692	compound	cold,challenge
R1708	T2694	T2687	punct	", ",was
R1709	T2695	T2696	det	the,temperature
R171	T141	T142	nummod	4,°C
R1710	T2696	T2687	nsubj	temperature,was
R1711	T2697	T2696	amod	average,temperature
R1712	T2698	T2696	amod	rectal,temperature
R1713	T2699	T2696	prep	of,temperature
R1714	T2700	T2701	nmod	MCAD,mice
R1715	T2701	T2699	pobj	mice,of
R1716	T2702	T2700	punct	−,MCAD
R1717	T2703	T2700	punct	/,MCAD
R1718	T2704	T2700	punct	−,MCAD
R1719	T2705	T2706	punct	(,5
R172	T142	T140	pobj	°C,at
R1720	T2706	T2701	parataxis	5,mice
R1721	T2707	T2706	nsubj	n,5
R1722	T2708	T2706	punct	=,5
R1723	T2709	T2706	punct	),5
R1724	T2710	T2711	nummod	23.4,°C
R1725	T2711	T2687	attr	°C,was
R1726	T2712	T2713	mark	as,compared
R1727	T2713	T2687	advcl	compared,was
R1728	T2714	T2713	prep	with,compared
R1729	T2715	T2716	nummod	35,°C
R173	T143	T136	prep	with,showed
R1730	T2716	T2714	pobj	°C,with
R1731	T2717	T2716	prep	for,°C
R1732	T2718	T2719	nmod	MCAD,mice
R1733	T2719	T2717	pobj	mice,for
R1734	T2720	T2718	punct	+,MCAD
R1735	T2721	T2718	punct	/,MCAD
R1736	T2722	T2718	punct	+,MCAD
R1737	T2723	T2724	punct	(,4
R1738	T2724	T2719	parataxis	4,mice
R1739	T2725	T2724	nsubj	n,4
R174	T144	T145	amod	prior,fasting
R1740	T2726	T2724	punct	=,4
R1741	T2727	T2724	punct	),4
R1742	T2728	T2687	punct	.,was
R1743	T2730	T2731	amod	Rectal,temperatures
R1744	T2731	T2732	nsubj	temperatures,declined
R1745	T2733	T2732	prep	to,declined
R1746	T2734	T2735	amod	unrecoverable,temperatures
R1747	T2735	T2733	pobj	temperatures,to
R1748	T2736	T2735	prep	of,temperatures
R1749	T2737	T2738	quantmod	16.7,19.2
R175	T145	T143	pobj	fasting,with
R1750	T2738	T2740	nummod	19.2,°C
R1751	T2739	T2738	punct	–,19.2
R1752	T2740	T2736	pobj	°C,of
R1753	T2741	T2732	prep	in,declined
R1754	T2742	T2743	quantmod	three,five
R1755	T2743	T2746	nummod	five,mice
R1756	T2744	T2743	quantmod	of,five
R1757	T2745	T2743	quantmod	the,five
R1758	T2746	T2741	pobj	mice,in
R1759	T2747	T2746	nmod	MCAD,mice
R176	T146	T127	punct	.,developed
R1760	T2748	T2747	punct	−,MCAD
R1761	T2749	T2747	punct	/,MCAD
R1762	T2750	T2747	punct	−,MCAD
R1763	T2751	T2732	punct	.,declined
R1764	T2753	T2754	prep	By,averaged
R1765	T2755	T2756	det	the,end
R1766	T2756	T2753	pobj	end,By
R1767	T2757	T2756	prep	of,end
R1768	T2758	T2759	det	the,mark
R1769	T2759	T2757	pobj	mark,of
R177	T148	T149	det	The,lesions
R1770	T2760	T2761	nummod	1.5,h
R1771	T2761	T2759	compound	h,mark
R1772	T2762	T2761	punct	-,h
R1773	T2763	T2754	punct	", ",averaged
R1774	T2764	T2765	det	the,mice
R1775	T2765	T2754	nsubj	mice,averaged
R1776	T2766	T2765	nummod	two,mice
R1777	T2767	T2765	amod	surviving,mice
R1778	T2768	T2765	nmod	MCAD,mice
R1779	T2769	T2768	punct	−,MCAD
R178	T149	T152	nsubjpass	lesions,reported
R1780	T2770	T2768	punct	/,MCAD
R1781	T2771	T2768	punct	−,MCAD
R1782	T2772	T2773	nummod	22.7,°C
R1783	T2773	T2754	dobj	°C,averaged
R1784	T2774	T2754	punct	.,averaged
R1785	T2776	T2777	prep	In,survived
R1786	T2778	T2776	pobj	contrast,In
R1787	T2779	T2777	punct	", ",survived
R1788	T2780	T2781	det	all,mice
R1789	T2781	T2777	nsubj	mice,survived
R179	T150	T149	amod	sporadic,lesions
R1790	T2782	T2781	nummod	four,mice
R1791	T2783	T2781	nmod	MCAD,mice
R1792	T2784	T2783	punct	+,MCAD
R1793	T2785	T2783	punct	/,MCAD
R1794	T2786	T2783	punct	+,MCAD
R1795	T2787	T2788	det	the,stress
R1796	T2788	T2777	dobj	stress,survived
R1797	T2789	T2790	nummod	3,h
R1798	T2790	T2788	compound	h,stress
R1799	T2791	T2790	punct	-,h
R18	T97	T98	det	a,model
R180	T151	T149	amod	cardiac,lesions
R1800	T2792	T2788	compound	cold,stress
R1801	T2793	T2777	punct	", ",survived
R1802	T2794	T2777	advcl	ending,survived
R1803	T2795	T2794	prep	with,ending
R1804	T2796	T2797	det	an,temperature
R1805	T2797	T2795	pobj	temperature,with
R1806	T2798	T2797	amod	average,temperature
R1807	T2799	T2797	amod	rectal,temperature
R1808	T2800	T2797	prep	of,temperature
R1809	T2801	T2802	nummod	33.6,°C
R181	T373	T374	amod	Mitochondrial,oxidation
R1810	T2802	T2800	pobj	°C,of
R1811	T2803	T2777	punct	.,survived
R1812	T2908	T2909	amod	Organic,Acid
R1813	T2910	T2909	cc	and,Acid
R1814	T2911	T2912	compound	Acylcarnitine,Analysis
R1815	T2912	T2909	conj	Analysis,Acid
R1816	T2914	T2915	nmod	Urine,acid
R1817	T2915	T2917	compound	acid,analysis
R1818	T2916	T2915	amod	organic,acid
R1819	T2917	T2918	nsubj	analysis,revealed
R182	T374	T377	nsubj	oxidation,provides
R1820	T2919	T2920	mark	that,developed
R1821	T2920	T2918	ccomp	developed,revealed
R1822	T2921	T2922	nmod	MCAD,mice
R1823	T2922	T2920	nsubj	mice,developed
R1824	T2923	T2921	punct	−,MCAD
R1825	T2924	T2921	punct	/,MCAD
R1826	T2925	T2921	punct	−,MCAD
R1827	T2926	T2927	det	an,profile
R1828	T2927	T2920	dobj	profile,developed
R1829	T2928	T2929	amod	organic,acid
R183	T375	T374	compound	β,oxidation
R1830	T2929	T2927	compound	acid,profile
R1831	T2930	T2927	amod	similar,profile
R1832	T2931	T2930	prep	to,similar
R1833	T2932	T2933	npadvmod	MCAD,deficient
R1834	T2933	T2935	amod	deficient,patients
R1835	T2934	T2933	punct	-,deficient
R1836	T2935	T2931	pobj	patients,to
R1837	T2936	T2935	amod	human,patients
R1838	T2937	T2918	punct	.,revealed
R1839	T2939	T2940	advmod	Specifically,developed
R184	T376	T374	punct	-,oxidation
R1840	T2941	T2940	punct	", ",developed
R1841	T2942	T2943	advmod	when,fasted
R1842	T2943	T2940	advcl	fasted,developed
R1843	T2944	T2943	prep	for,fasted
R1844	T2945	T2946	nummod	18,h
R1845	T2946	T2944	pobj	h,for
R1846	T2947	T2940	punct	", ",developed
R1847	T2948	T2949	nmod	MCAD,mice
R1848	T2949	T2940	nsubj	mice,developed
R1849	T2950	T2948	punct	−,MCAD
R185	T378	T374	prep	of,oxidation
R1850	T2951	T2948	punct	/,MCAD
R1851	T2952	T2948	punct	−,MCAD
R1852	T2953	T2954	advmod	significantly,elevated
R1853	T2954	T2955	amod	elevated,concentrations
R1854	T2955	T2940	dobj	concentrations,developed
R1855	T2956	T2955	prep	of,concentrations
R1856	T2957	T2958	amod	adipic,acids
R1857	T2958	T2956	pobj	acids,of
R1858	T2959	T2957	punct	", ",adipic
R1859	T2960	T2957	conj	suberic,adipic
R186	T379	T380	amod	fatty,acids
R1860	T2961	T2960	punct	", ",suberic
R1861	T2962	T2960	cc	and,suberic
R1862	T2963	T2960	conj	sebacic,suberic
R1863	T2964	T2958	cc	and,acids
R1864	T2965	T2958	conj	hexanoylglycine,acids
R1865	T2966	T2967	mark	as,compared
R1866	T2967	T2940	advcl	compared,developed
R1867	T2968	T2967	prep	to,compared
R1868	T2969	T2970	nmod	MCAD,controls
R1869	T2970	T2968	pobj	controls,to
R187	T380	T378	pobj	acids,of
R1870	T2971	T2969	punct	+,MCAD
R1871	T2972	T2969	punct	/,MCAD
R1872	T2973	T2969	punct	+,MCAD
R1873	T2974	T2970	punct	", ",controls
R1874	T2975	T2976	dep	which,showed
R1875	T2976	T2970	relcl	showed,controls
R1876	T2977	T2976	dobj	trace,showed
R1877	T2978	T2977	prep	to,trace
R1878	T2979	T2980	det	no,amounts
R1879	T2980	T2978	pobj	amounts,to
R188	T381	T377	dobj	energy,provides
R1880	T2981	T2980	amod	detectable,amounts
R1881	T2982	T2977	prep	of,trace
R1882	T2983	T2984	det	the,acids
R1883	T2984	T2982	pobj	acids,of
R1884	T2985	T2984	amod	same,acids
R1885	T2986	T2984	amod	organic,acids
R1886	T2987	T2988	punct	(,Table
R1887	T2988	T2940	parataxis	Table,developed
R1888	T2989	T2988	nummod	1,Table
R1889	T2990	T2988	punct	),Table
R189	T382	T377	punct	", ",provides
R1890	T2991	T2940	punct	.,developed
R1891	T2993	T2994	amod	Adipic,acid
R1892	T2994	T2995	nsubj	acid,is
R1893	T2996	T2995	neg	not,is
R1894	T2997	T2995	acomp	specific,is
R1895	T2998	T2997	prep	to,specific
R1896	T2999	T3000	compound	MCAD,deficiency
R1897	T3000	T2998	pobj	deficiency,to
R1898	T3001	T2995	punct	.,is
R1899	T3003	T3004	nsubj	We,evaluated
R19	T98	T89	dobj	model,developed
R190	T383	T384	advmod	especially,during
R1900	T3005	T3004	advmod	also,evaluated
R1901	T3006	T3007	npadvmod	β,hydroxybutyric
R1902	T3007	T3009	amod	hydroxybutyric,concentrations
R1903	T3008	T3007	punct	-,hydroxybutyric
R1904	T3009	T3004	dobj	concentrations,evaluated
R1905	T3010	T3007	cc	and,hydroxybutyric
R1906	T3011	T3007	conj	acetoacetic,hydroxybutyric
R1907	T3012	T3004	cc	and,evaluated
R1908	T3013	T3004	conj	found,evaluated
R1909	T3014	T3015	det	no,differences
R191	T384	T377	prep	during,provides
R1910	T3015	T3013	dobj	differences,found
R1911	T3016	T3015	amod	significant,differences
R1912	T3017	T3015	prep	between,differences
R1913	T3018	T3019	compound	MCAD,genotypes
R1914	T3019	T3017	pobj	genotypes,between
R1915	T3020	T3021	punct	(,shown
R1916	T3021	T3013	parataxis	shown,found
R1917	T3022	T3021	nsubj	data,shown
R1918	T3023	T3021	neg	not,shown
R1919	T3024	T3021	punct	),shown
R192	T385	T386	compound	fasting,conditions
R1920	T3025	T3004	punct	.,evaluated
R1921	T3027	T3028	nsubj	Comparison,revealed
R1922	T3029	T3027	prep	of,Comparison
R1923	T3030	T3031	nmod	MCAD,mice
R1924	T3031	T3029	pobj	mice,of
R1925	T3032	T3030	punct	+,MCAD
R1926	T3033	T3030	punct	/,MCAD
R1927	T3034	T3030	punct	+,MCAD
R1928	T3035	T3030	cc	and,MCAD
R1929	T3036	T3030	conj	MCAD,MCAD
R193	T386	T384	pobj	conditions,during
R1930	T3037	T3036	punct	−,MCAD
R1931	T3038	T3036	punct	/,MCAD
R1932	T3039	T3036	punct	−,MCAD
R1933	T3040	T3041	det	no,differences
R1934	T3041	T3028	dobj	differences,revealed
R1935	T3042	T3041	amod	significant,differences
R1936	T3043	T3041	prep	in,differences
R1937	T3044	T3045	amod	total,concentrations
R1938	T3045	T3043	pobj	concentrations,in
R1939	T3046	T3045	compound	serum,concentrations
R194	T387	T377	punct	.,provides
R1940	T3047	T3045	compound	carnitine,concentrations
R1941	T3048	T3041	prep	between,differences
R1942	T3049	T3050	nmod	MCAD,mice
R1943	T3050	T3048	pobj	mice,between
R1944	T3051	T3049	punct	+,MCAD
R1945	T3052	T3049	punct	/,MCAD
R1946	T3053	T3049	punct	+,MCAD
R1947	T3054	T3049	cc	and,MCAD
R1948	T3055	T3049	conj	MCAD,MCAD
R1949	T3056	T3055	punct	−,MCAD
R195	T389	T390	amod	Fatty,acid
R1950	T3057	T3055	punct	/,MCAD
R1951	T3058	T3055	punct	−,MCAD
R1952	T3059	T3028	punct	", ",revealed
R1953	T3060	T3028	cc	but,revealed
R1954	T3061	T3062	nmod	MCAD,mice
R1955	T3062	T3066	nsubj	mice,had
R1956	T3063	T3061	punct	−,MCAD
R1957	T3064	T3061	punct	/,MCAD
R1958	T3065	T3061	punct	−,MCAD
R1959	T3066	T3028	conj	had,revealed
R196	T390	T391	compound	acid,oxidation
R1960	T3067	T3068	det	a,elevation
R1961	T3068	T3075	nsubj	elevation,evident
R1962	T3069	T3070	quantmod	5,6
R1963	T3070	T3073	quantmod	6,fold
R1964	T3071	T3070	punct	-,6
R1965	T3072	T3070	quantmod	to,6
R1966	T3073	T3068	nummod	fold,elevation
R1967	T3074	T3073	punct	-,fold
R1968	T3075	T3066	ccomp	evident,had
R1969	T3076	T3068	prep	of,elevation
R197	T391	T392	nsubj	oxidation,occurs
R1970	T3077	T3078	compound	serum,decenoylcarnitine
R1971	T3078	T3076	pobj	decenoylcarnitine,of
R1972	T3079	T3075	prep	in,evident
R1973	T3080	T3081	det	the,profile
R1974	T3081	T3079	pobj	profile,in
R1975	T3082	T3081	compound	acylcarnitine,profile
R1976	T3083	T3084	punct	(,Figure
R1977	T3084	T3066	parataxis	Figure,had
R1978	T3085	T3084	nummod	4A,Figure
R1979	T3086	T3084	punct	),Figure
R198	T393	T392	prep	in,occurs
R1980	T3087	T3066	punct	.,had
R1981	T3089	T3090	compound	Bile,acylcarnitine
R1982	T3090	T3091	compound	acylcarnitine,analysis
R1983	T3091	T3092	nsubj	analysis,revealed
R1984	T3093	T3094	det	a,pattern
R1985	T3094	T3092	dobj	pattern,revealed
R1986	T3095	T3094	amod	similar,pattern
R1987	T3096	T3094	compound	acylcarnitine,pattern
R1988	T3097	T3094	prep	as,pattern
R1989	T3098	T3097	prep	in,as
R199	T394	T393	pobj	mitochondria,in
R1990	T3099	T3098	pobj	serum,in
R1991	T3100	T3101	punct	(,Figure
R1992	T3101	T3092	parataxis	Figure,revealed
R1993	T3102	T3101	nummod	4B,Figure
R1994	T3103	T3101	punct	),Figure
R1995	T3104	T3092	punct	.,revealed
R1996	T3106	T3107	advmod	However,are
R1997	T3108	T3107	punct	", ",are
R1998	T3109	T3110	det	the,profiles
R1999	T3110	T3107	nsubj	profiles,are
R2	T79	T77	compound	acid,oxidation
R20	T99	T98	compound	mouse,model
R200	T395	T392	cc	and,occurs
R2000	T3111	T3110	compound	acylcarnitine,profiles
R2001	T3112	T3110	prep	of,profiles
R2002	T3113	T3114	det	the,mice
R2003	T3114	T3112	pobj	mice,of
R2004	T3115	T3114	nmod	MCAD,mice
R2005	T3116	T3115	punct	−,MCAD
R2006	T3117	T3115	punct	/,MCAD
R2007	T3118	T3115	punct	−,MCAD
R2008	T3119	T3107	acomp	different,are
R2009	T3120	T3119	prep	from,different
R201	T396	T392	conj	consists,occurs
R2010	T3121	T3120	pobj	those,from
R2011	T3122	T3121	prep	of,those
R2012	T3123	T3124	amod	human,patients
R2013	T3124	T3122	pobj	patients,of
R2014	T3125	T3126	npadvmod	MCAD,deficient
R2015	T3126	T3124	amod	deficient,patients
R2016	T3127	T3126	punct	-,deficient
R2017	T3128	T3129	punct	(,Figure
R2018	T3129	T3107	parataxis	Figure,are
R2019	T3130	T3129	nummod	4C,Figure
R202	T397	T396	prep	of,consists
R2020	T3131	T3129	punct	),Figure
R2021	T3132	T3107	punct	.,are
R2022	T3134	T3135	amod	Human,patients
R2023	T3135	T3136	nsubj	patients,present
R2024	T3136	T3137	ccomp	present,was
R2025	T3138	T3136	prep	with,present
R2026	T3139	T3140	amod	elevated,levels
R2027	T3140	T3138	pobj	levels,with
R2028	T3141	T3140	prep	of,levels
R2029	T3142	T3141	pobj	C6,of
R203	T398	T399	det	a,circuit
R2030	T3143	T3142	punct	", ",C6
R2031	T3144	T3142	conj	C8,C6
R2032	T3145	T3144	punct	", ",C8
R2033	T3146	T3144	cc	and,C8
R2034	T3147	T3144	conj	C10,C8
R2035	T3148	T3147	punct	:,C10
R2036	T3149	T3147	nummod	1,C10
R2037	T3150	T3142	appos	acylcarnitines,C6
R2038	T3151	T3136	punct	", ",present
R2039	T3152	T3153	mark	as,did
R204	T399	T397	pobj	circuit,of
R2040	T3153	T3136	advcl	did,present
R2041	T3154	T3155	det	the,mice
R2042	T3155	T3153	nsubj	mice,did
R2043	T3156	T3155	compound	mutant,mice
R2044	T3157	T3137	punct	;,was
R2045	T3158	T3137	advmod	however,was
R2046	T3159	T3137	punct	", ",was
R2047	T3160	T3161	det	the,peak
R2048	T3161	T3137	nsubj	peak,was
R2049	T3162	T3161	amod	predominant,peak
R205	T400	T399	amod	repeating,circuit
R2050	T3163	T3164	compound	C8,acylcarnitine
R2051	T3164	T3137	attr	acylcarnitine,was
R2052	T3165	T3137	prep	in,was
R2053	T3166	T3165	pobj	humans,in
R2054	T3167	T3137	punct	", ",was
R2055	T3168	T3169	mark	whereas,was
R2056	T3169	T3137	advcl	was,was
R2057	T3170	T3169	prep	in,was
R2058	T3171	T3172	det	the,mouse
R2059	T3172	T3170	pobj	mouse,in
R206	T401	T399	prep	of,circuit
R2060	T3173	T3169	nsubj	it,was
R2061	T3174	T3175	nmod	C10,acylcarnitine
R2062	T3175	T3169	attr	acylcarnitine,was
R2063	T3176	T3174	punct	:,C10
R2064	T3177	T3174	nummod	1,C10
R2065	T3178	T3137	punct	.,was
R207	T402	T403	nummod	four,steps
R2070	T3319	T3320	amod	Complete,examination
R2071	T3320	T3322	nsubj	examination,demonstrated
R2072	T3321	T3320	amod	histopathologic,examination
R2073	T3323	T3320	prep	of,examination
R2074	T3324	T3325	nummod	one,group
R2075	T3325	T3323	pobj	group,of
R2076	T3326	T3325	prep	of,group
R2077	T3327	T3328	nmod	mutant,mice
R2078	T3328	T3326	pobj	mice,of
R2079	T3329	T3327	cc	and,mutant
R208	T403	T401	pobj	steps,of
R2080	T3330	T3327	conj	MCAD,mutant
R2081	T3331	T3330	punct	+,MCAD
R2082	T3332	T3330	punct	/,MCAD
R2083	T3333	T3330	punct	+,MCAD
R2084	T3334	T3328	compound	control,mice
R2085	T3335	T3320	prep	after,examination
R2086	T3336	T3337	det	a,fast
R2087	T3337	T3335	pobj	fast,after
R2088	T3338	T3339	nummod	24,h
R2089	T3339	T3337	compound	h,fast
R209	T404	T403	amod	sequential,steps
R2090	T3340	T3339	punct	-,h
R2091	T3341	T3342	amod	diffuse,steatosis
R2092	T3342	T3322	dobj	steatosis,demonstrated
R2093	T3343	T3342	amod	microvesicular,steatosis
R2094	T3344	T3343	cc	and,microvesicular
R2095	T3345	T3343	conj	macrovesicular,microvesicular
R2096	T3346	T3342	amod	hepatic,steatosis
R2097	T3347	T3322	prep	in,demonstrated
R2098	T3348	T3349	quantmod	6,8
R2099	T3349	T3351	nummod	8,wk
R21	T100	T98	prep	for,model
R210	T405	T392	punct	.,occurs
R2100	T3350	T3349	punct	–,8
R2101	T3351	T3353	npadvmod	wk,old
R2102	T3352	T3351	punct	-,wk
R2103	T3353	T3355	amod	old,mice
R2104	T3354	T3353	punct	-,old
R2105	T3355	T3347	pobj	mice,in
R2106	T3356	T3355	nmod	MCAD,mice
R2107	T3357	T3356	punct	−,MCAD
R2108	T3358	T3356	punct	/,MCAD
R2109	T3359	T3356	punct	−,MCAD
R211	T407	T408	expl	There,are
R2110	T3360	T3361	mark	whereas,had
R2111	T3361	T3322	advcl	had,demonstrated
R2112	T3362	T3363	nmod	MCAD,mice
R2113	T3363	T3361	nsubj	mice,had
R2114	T3364	T3362	punct	+,MCAD
R2115	T3365	T3362	punct	/,MCAD
R2116	T3366	T3362	punct	+,MCAD
R2117	T3367	T3368	det	no,changes
R2118	T3368	T3361	dobj	changes,had
R2119	T3369	T3368	amod	histologic,changes
R212	T409	T410	nummod	four,dehydrogenases
R2120	T3370	T3371	punct	(,5A
R2121	T3371	T3322	parataxis	5A,demonstrated
R2122	T3372	T3371	nmod	Figure,5A
R2123	T3373	T3371	cc	and,5A
R2124	T3374	T3371	conj	5B,5A
R2125	T3375	T3371	punct	),5A
R2126	T3376	T3322	punct	.,demonstrated
R2127	T3378	T3379	prep	In,were
R2128	T3380	T3381	det	another,group
R2129	T3381	T3378	pobj	group,In
R213	T410	T408	attr	dehydrogenases,are
R2130	T3382	T3381	prep	of,group
R2131	T3383	T3384	nummod	4,wk
R2132	T3384	T3386	npadvmod	wk,old
R2133	T3385	T3384	punct	-,wk
R2134	T3386	T3388	amod	old,mice
R2135	T3387	T3386	punct	-,old
R2136	T3388	T3382	pobj	mice,of
R2137	T3389	T3388	nmod	MCAD,mice
R2138	T3390	T3389	punct	+,MCAD
R2139	T3391	T3389	punct	/,MCAD
R214	T411	T412	amod	straight,chain
R2140	T3392	T3389	punct	+,MCAD
R2141	T3393	T3389	cc	and,MCAD
R2142	T3394	T3389	conj	MCAD,MCAD
R2143	T3395	T3394	punct	−,MCAD
R2144	T3396	T3394	punct	/,MCAD
R2145	T3397	T3394	punct	−,MCAD
R2146	T3398	T3388	acl	fasted,mice
R2147	T3399	T3398	prep	for,fasted
R2148	T3400	T3401	nummod	24,h
R2149	T3401	T3399	pobj	h,for
R215	T412	T410	compound	chain,dehydrogenases
R2150	T3402	T3401	punct	-,h
R2151	T3403	T3379	punct	", ",were
R2152	T3404	T3379	expl	there,were
R2153	T3405	T3379	attr	minimal,were
R2154	T3406	T3405	prep	to,minimal
R2155	T3407	T3406	pobj	no,to
R2156	T3408	T3405	appos	abnormalities,minimal
R2157	T3409	T3379	prep	in,were
R2158	T3410	T3411	det	all,organs
R2159	T3411	T3409	pobj	organs,in
R216	T413	T412	punct	-,chain
R2160	T3412	T3411	acl	evaluated,organs
R2161	T3413	T3379	punct	.,were
R2162	T3415	T3416	advmod	Only,mice
R2163	T3416	T3423	nsubj	mice,demonstrated
R2164	T3417	T3416	det	the,mice
R2165	T3418	T3416	amod	older,mice
R2166	T3419	T3416	nmod	MCAD,mice
R2167	T3420	T3419	punct	−,MCAD
R2168	T3421	T3419	punct	/,MCAD
R2169	T3422	T3419	punct	−,MCAD
R217	T414	T415	compound	acyl,CoA
R2170	T3424	T3423	punct	", ",demonstrated
R2171	T3425	T3423	advmod	therefore,demonstrated
R2172	T3426	T3423	punct	", ",demonstrated
R2173	T3427	T3428	amod	hepatic,steatosis
R2174	T3428	T3423	dobj	steatosis,demonstrated
R2175	T3429	T3423	punct	.,demonstrated
R2176	T3431	T3432	prep	In,found
R2177	T3433	T3431	pobj	addition,In
R2178	T3434	T3432	punct	", ",found
R2179	T3435	T3432	nsubj	we,found
R218	T415	T410	compound	CoA,dehydrogenases
R2180	T3436	T3437	amod	sporadic,lesions
R2181	T3437	T3432	dobj	lesions,found
R2182	T3438	T3437	amod	cardiac,lesions
R2183	T3439	T3432	prep	in,found
R2184	T3440	T3441	amod	multiple,mice
R2185	T3441	T3439	pobj	mice,in
R2186	T3442	T3441	nmod	MCAD,mice
R2187	T3443	T3442	punct	−,MCAD
R2188	T3444	T3442	punct	/,MCAD
R2189	T3445	T3442	punct	−,MCAD
R219	T416	T415	punct	-,CoA
R2190	T3446	T3432	punct	.,found
R2191	T3448	T3449	prep	In,had
R2192	T3450	T3451	nummod	one,example
R2193	T3451	T3448	pobj	example,In
R2194	T3452	T3449	punct	", ",had
R2195	T3453	T3454	det	an,mouse
R2196	T3454	T3449	nsubj	mouse,had
R2197	T3455	T3454	nmod	MCAD,mouse
R2198	T3456	T3455	punct	−,MCAD
R2199	T3457	T3455	punct	/,MCAD
R22	T101	T102	compound	MCAD,deficiency
R220	T417	T410	acl	involved,dehydrogenases
R2200	T3458	T3455	punct	−,MCAD
R2201	T3459	T3460	amod	diffuse,cardiomyopathy
R2202	T3460	T3449	dobj	cardiomyopathy,had
R2203	T3461	T3460	prep	with,cardiomyopathy
R2204	T3462	T3463	amod	chronic,degeneration
R2205	T3463	T3461	pobj	degeneration,with
R2206	T3464	T3463	amod	active,degeneration
R2207	T3465	T3463	amod	multifocal,degeneration
R2208	T3466	T3463	compound	myocyte,degeneration
R2209	T3467	T3463	cc	and,degeneration
R221	T418	T417	prep	in,involved
R2210	T3468	T3463	conj	necrosis,degeneration
R2211	T3469	T3470	punct	(,5C
R2212	T3470	T3449	parataxis	5C,had
R2213	T3471	T3470	nmod	Figure,5C
R2214	T3472	T3470	cc	and,5C
R2215	T3473	T3470	conj	5D,5C
R2216	T3474	T3470	punct	),5C
R2217	T3475	T3449	punct	.,had
R2218	T3477	T3478	nsubj	Changes,included
R2219	T3479	T3477	prep	in,Changes
R222	T419	T420	det	the,step
R2220	T3480	T3481	amod	degenerating,myocytes
R2221	T3481	T3479	pobj	myocytes,in
R2222	T3482	T3478	dobj	swelling,included
R2223	T3483	T3482	punct	", ",swelling
R2224	T3484	T3485	amod	pale,staining
R2225	T3485	T3482	conj	staining,swelling
R2226	T3486	T3485	punct	", ",staining
R2227	T3487	T3485	cc	and,staining
R2228	T3488	T3485	punct	", ",staining
R2229	T3489	T3490	prep	in,replacement
R223	T420	T418	pobj	step,in
R2230	T3490	T3485	conj	replacement,staining
R2231	T3491	T3489	pobj	portions,in
R2232	T3492	T3491	prep	of,portions
R2233	T3493	T3494	det	the,sarcoplasm
R2234	T3494	T3492	pobj	sarcoplasm,of
R2235	T3495	T3490	punct	", ",replacement
R2236	T3496	T3490	prep	of,replacement
R2237	T3497	T3496	pobj	myofibrils,of
R2238	T3498	T3490	prep	with,replacement
R2239	T3499	T3500	advmod	finely,granular
R224	T421	T420	amod	initial,step
R2240	T3500	T3501	amod	granular,material
R2241	T3501	T3498	pobj	material,with
R2242	T3502	T3501	amod	eosinophilic,material
R2243	T3503	T3478	punct	.,included
R2244	T3505	T3506	nsubj	Nuclei,were
R2245	T3507	T3505	prep	of,Nuclei
R2246	T3508	T3509	amod	affected,myocytes
R2247	T3509	T3507	pobj	myocytes,of
R2248	T3510	T3506	acomp	large,were
R2249	T3511	T3510	punct	", ",large
R225	T422	T408	punct	.,are
R2250	T3512	T3510	conj	pale,large
R2251	T3513	T3512	punct	", ",pale
R2252	T3514	T3512	cc	and,pale
R2253	T3515	T3512	conj	vesicular,pale
R2254	T3516	T3506	cc	and,were
R2255	T3517	T3506	conj	had,were
R2256	T3518	T3519	amod	prominent,nucleoli
R2257	T3519	T3517	dobj	nucleoli,had
R2258	T3520	T3506	punct	.,were
R2259	T3522	T3523	prep	In,was
R226	T424	T425	amod	Medium,chain
R2260	T3524	T3525	det	the,areas
R2261	T3525	T3522	pobj	areas,In
R2262	T3526	T3527	advmod	most,severely
R2263	T3527	T3528	advmod	severely,affected
R2264	T3528	T3525	amod	affected,areas
R2265	T3529	T3523	punct	", ",was
R2266	T3530	T3523	expl	there,was
R2267	T3531	T3523	attr	loss,was
R2268	T3532	T3531	prep	of,loss
R2269	T3533	T3532	pobj	myocytes,of
R227	T425	T427	compound	chain,dehydrogenase
R2270	T3534	T3531	acl	accompanied,loss
R2271	T3535	T3534	agent	by,accompanied
R2272	T3536	T3535	pobj	fibrosis,by
R2273	T3537	T3523	punct	.,was
R2274	T3539	T3540	prep	In,was
R2275	T3541	T3542	det	the,wall
R2276	T3542	T3539	pobj	wall,In
R2277	T3543	T3542	prep	of,wall
R2278	T3544	T3545	det	the,aorta
R2279	T3545	T3543	pobj	aorta,of
R228	T426	T425	punct	-,chain
R2280	T3546	T3545	prep	at,aorta
R2281	T3547	T3548	det	the,base
R2282	T3548	T3546	pobj	base,at
R2283	T3549	T3548	prep	of,base
R2284	T3550	T3551	det	the,heart
R2285	T3551	T3549	pobj	heart,of
R2286	T3552	T3540	expl	there,was
R2287	T3553	T3554	amod	multifocal,degeneration
R2288	T3554	T3540	attr	degeneration,was
R2289	T3555	T3554	prep	of,degeneration
R229	T427	T431	nsubj	dehydrogenase,is
R2290	T3556	T3557	det	the,tissue
R2291	T3557	T3555	pobj	tissue,of
R2292	T3558	T3557	amod	elastic,tissue
R2293	T3559	T3554	punct	", ",degeneration
R2294	T3560	T3554	acl	accompanied,degeneration
R2295	T3561	T3560	agent	by,accompanied
R2296	T3562	T3563	amod	multifocal,collections
R2297	T3563	T3561	pobj	collections,by
R2298	T3564	T3563	prep	of,collections
R2299	T3565	T3566	amod	globular,pigment
R23	T102	T100	pobj	deficiency,for
R230	T428	T429	compound	acyl,CoA
R2300	T3566	T3564	pobj	pigment,of
R2301	T3567	T3566	amod	translucent,pigment
R2302	T3568	T3569	amod	yellow,brown
R2303	T3569	T3566	amod	brown,pigment
R2304	T3570	T3569	punct	-,brown
R2305	T3571	T3563	acl	interpreted,collections
R2306	T3572	T3573	aux	to,be
R2307	T3573	T3571	xcomp	be,interpreted
R2308	T3574	T3575	compound	ceroid,lipofuscin
R2309	T3575	T3573	attr	lipofuscin,be
R231	T429	T427	compound	CoA,dehydrogenase
R2310	T3576	T3575	punct	/,lipofuscin
R2311	T3577	T3540	punct	.,was
R2312	T3579	T3580	amod	Similar,deposits
R2313	T3580	T3581	nsubjpass	deposits,scattered
R2314	T3582	T3581	auxpass	were,scattered
R2315	T3583	T3581	prep	within,scattered
R2316	T3584	T3585	amod	adjacent,tissue
R2317	T3585	T3583	pobj	tissue,within
R2318	T3586	T3585	compound	adipose,tissue
R2319	T3587	T3581	punct	.,scattered
R232	T430	T429	punct	-,CoA
R2322	T3809	T3810	advmod	Successfully,targeting
R2323	T3810	T3811	csubj	targeting,produced
R2324	T3812	T3810	dobj	Acadm,targeting
R2325	T3813	T3814	det	a,model
R2326	T3814	T3811	dobj	model,produced
R2327	T3815	T3814	compound	mouse,model
R2328	T3816	T3814	prep	for,model
R2329	T3817	T3818	compound	MCAD,deficiency
R233	T432	T427	punct	(,dehydrogenase
R2330	T3818	T3816	pobj	deficiency,for
R2331	T3819	T3814	prep	with,model
R2332	T3820	T3819	pobj	features,with
R2333	T3821	T3822	dep	that,mimic
R2334	T3822	T3820	relcl	mimic,features
R2335	T3823	T3824	det	the,phenotype
R2336	T3824	T3822	dobj	phenotype,mimic
R2337	T3825	T3824	amod	clinical,phenotype
R2338	T3826	T3825	punct	", ",clinical
R2339	T3827	T3825	conj	biochemical,clinical
R234	T433	T427	appos	MCAD,dehydrogenase
R2340	T3828	T3827	punct	", ",biochemical
R2341	T3829	T3827	cc	and,biochemical
R2342	T3830	T3827	conj	pathologic,biochemical
R2343	T3831	T3824	acl	found,phenotype
R2344	T3832	T3831	prep	in,found
R2345	T3833	T3834	amod	human,patients
R2346	T3834	T3832	pobj	patients,in
R2347	T3835	T3811	punct	.,produced
R2348	T3837	T3838	npadvmod	MCAD,deficient
R2349	T3838	T3840	amod	deficient,patients
R235	T434	T427	punct	),dehydrogenase
R2350	T3839	T3838	punct	-,deficient
R2351	T3840	T3841	nsubj	patients,have
R2352	T3842	T3843	amod	abnormal,metabolites
R2353	T3843	T3841	dobj	metabolites,have
R2354	T3844	T3843	nmod	plasma,metabolites
R2355	T3845	T3844	cc	and,plasma
R2356	T3846	T3844	conj	urine,plasma
R2357	T3847	T3843	acl	associated,metabolites
R2358	T3848	T3847	prep	with,associated
R2359	T3849	T3850	det	the,specificity
R236	T435	T436	punct	(,is
R2360	T3850	T3848	pobj	specificity,with
R2361	T3851	T3852	amod	medium,chain
R2362	T3852	T3853	compound	chain,length
R2363	T3853	T3850	compound	length,specificity
R2364	T3854	T3853	punct	–,length
R2365	T3855	T3850	compound	enzyme,specificity
R2366	T3856	T3841	punct	.,have
R2367	T3858	T3859	npadvmod	MCAD,deficient
R2368	T3859	T3861	amod	deficient,patients
R2369	T3860	T3859	punct	-,deficient
R237	T436	T427	parataxis	is,dehydrogenase
R2370	T3861	T3862	nsubj	patients,display
R2371	T3863	T3864	punct	[,15
R2372	T3864	T3861	parataxis	15,patients
R2373	T3865	T3864	punct	],15
R2374	T3866	T3862	advmod	often,display
R2375	T3867	T3868	det	a,peak
R2376	T3868	T3862	dobj	peak,display
R2377	T3869	T3868	amod	characteristic,peak
R2378	T3870	T3868	amod	urinary,peak
R2379	T3871	T3868	compound	hexanoylglycine,peak
R238	T437	T438	det	the,gene
R2380	T3872	T3862	punct	", ",display
R2381	T3873	T3874	mark	as,seen
R2382	T3874	T3862	advcl	seen,display
R2383	T3875	T3874	auxpass	was,seen
R2384	T3876	T3874	prep	in,seen
R2385	T3877	T3878	nmod	MCAD,mice
R2386	T3878	T3876	pobj	mice,in
R2387	T3879	T3877	punct	−,MCAD
R2388	T3880	T3877	punct	/,MCAD
R2389	T3881	T3877	punct	−,MCAD
R239	T438	T436	nsubj	gene,is
R2390	T3882	T3862	punct	.,display
R2391	T3884	T3885	compound	Acylcarnitine,analysis
R2392	T3885	T3886	nsubj	analysis,indicated
R2393	T3887	T3886	punct	", ",indicated
R2394	T3888	T3886	advmod	however,indicated
R2395	T3889	T3886	punct	", ",indicated
R2396	T3890	T3891	compound	mouse,MCAD
R2397	T3891	T3892	nsubj	MCAD,is
R2398	T3892	T3886	advcl	is,indicated
R2399	T3893	T3894	advmod	more,active
R24	T103	T102	punct	(,deficiency
R240	T439	T438	compound	mouse,gene
R2400	T3894	T3892	acomp	active,is
R2401	T3895	T3894	prep	toward,active
R2402	T3896	T3897	amod	longer,substrates
R2403	T3897	T3895	pobj	substrates,toward
R2404	T3898	T3897	compound	chain,substrates
R2405	T3899	T3894	prep	than,active
R2406	T3900	T3901	det	the,enzyme
R2407	T3901	T3899	pobj	enzyme,than
R2408	T3902	T3901	amod	human,enzyme
R2409	T3903	T3901	compound	MCAD,enzyme
R241	T440	T436	attr	Acadm,is
R2410	T3904	T3886	punct	.,indicated
R2411	T3906	T3907	det	This,finding
R2412	T3907	T3908	nsubj	finding,is
R2413	T3909	T3908	acomp	similar,is
R2414	T3910	T3909	prep	to,similar
R2415	T3911	T3910	pobj	that,to
R2416	T3912	T3911	acl	seen,that
R2417	T3913	T3912	prep	with,seen
R2418	T3914	T3915	advmod	very,long
R2419	T3915	T3916	amod	long,chain
R242	T441	T436	punct	", ",is
R2420	T3916	T3918	compound	chain,dehydrogenase
R2421	T3917	T3916	punct	-,chain
R2422	T3918	T3913	pobj	dehydrogenase,with
R2423	T3919	T3920	compound	acyl,CoA
R2424	T3920	T3918	compound	CoA,dehydrogenase
R2425	T3921	T3920	punct	-,CoA
R2426	T3922	T3918	punct	(,dehydrogenase
R2427	T3923	T3918	appos	VLCAD,dehydrogenase
R2428	T3924	T3918	punct	),dehydrogenase
R2429	T3925	T3926	advmod	where,is
R243	T442	T443	mark	whereas,is
R2430	T3926	T3918	relcl	is,dehydrogenase
R2431	T3927	T3928	compound	mouse,VLCAD
R2432	T3928	T3926	nsubj	VLCAD,is
R2433	T3929	T3930	advmod	most,active
R2434	T3930	T3926	acomp	active,is
R2435	T3931	T3930	prep	toward,active
R2436	T3932	T3933	compound	C16,substrates
R2437	T3933	T3931	pobj	substrates,toward
R2438	T3934	T3933	compound	acyl,substrates
R2439	T3935	T3933	punct	-,substrates
R244	T443	T436	advcl	is,is
R2440	T3936	T3937	mark	as,compared
R2441	T3937	T3926	advcl	compared,is
R2442	T3938	T3937	prep	to,compared
R2443	T3939	T3940	amod	human,VLCAD
R2444	T3940	T3938	pobj	VLCAD,to
R2445	T3941	T3940	prep	with,VLCAD
R2446	T3942	T3943	det	the,activity
R2447	T3943	T3941	pobj	activity,with
R2448	T3944	T3943	advmod	most,activity
R2449	T3945	T3943	amod	enzymatic,activity
R245	T444	T445	det	the,protein
R2450	T3946	T3943	prep	toward,activity
R2451	T3947	T3948	compound	C14,substrates
R2452	T3948	T3946	pobj	substrates,toward
R2453	T3949	T3948	compound	acyl,substrates
R2454	T3950	T3948	punct	-,substrates
R2455	T3951	T3952	punct	[,16
R2456	T3952	T3908	parataxis	16,is
R2457	T3953	T3952	punct	],16
R2458	T3954	T3908	punct	.,is
R2459	T3956	T3957	compound	ETF,assays
R246	T445	T443	nsubj	protein,is
R2460	T3957	T3959	nsubjpass	assays,performed
R2461	T3958	T3957	compound	reduction,assays
R2462	T3960	T3957	prep	of,assays
R2463	T3961	T3962	compound	liver,homogenates
R2464	T3962	T3960	pobj	homogenates,of
R2465	T3963	T3959	auxpass	were,performed
R2466	T3964	T3965	aux	to,characterize
R2467	T3965	T3959	advcl	characterize,performed
R2468	T3966	T3967	det	the,mice
R2469	T3967	T3965	dobj	mice,characterize
R247	T446	T443	attr	MCAD,is
R2470	T3968	T3967	nmod	MCAD,mice
R2471	T3969	T3968	punct	−,MCAD
R2472	T3970	T3968	punct	/,MCAD
R2473	T3971	T3968	punct	−,MCAD
R2474	T3972	T3965	prep	at,characterize
R2475	T3973	T3974	det	the,level
R2476	T3974	T3972	pobj	level,at
R2477	T3975	T3974	amod	enzymatic,level
R2478	T3976	T3959	punct	.,performed
R2479	T3978	T3979	nmod	MCAD,mice
R248	T447	T436	punct	),is
R2480	T3979	T3983	nsubj	mice,had
R2481	T3980	T3978	punct	−,MCAD
R2482	T3981	T3978	punct	/,MCAD
R2483	T3982	T3978	punct	−,MCAD
R2484	T3984	T3985	det	a,ability
R2485	T3985	T3983	dobj	ability,had
R2486	T3986	T3987	advmod	significantly,reduced
R2487	T3987	T3985	amod	reduced,ability
R2488	T3988	T3989	aux	to,dehydrogenate
R2489	T3989	T3985	acl	dehydrogenate,ability
R249	T448	T431	punct	", ",is
R2490	T3990	T3991	compound	C8,CoA
R2491	T3991	T3989	dobj	CoA,dehydrogenate
R2492	T3992	T3991	punct	-,CoA
R2493	T3993	T3983	punct	", ",had
R2494	T3994	T3995	mark	as,is
R2495	T3995	T3983	advcl	is,had
R2496	T3996	T3997	det	the,case
R2497	T3997	T3995	attr	case,is
R2498	T3998	T3995	prep	in,is
R2499	T3999	T4000	amod	human,patients
R25	T104	T102	appos	MCAD,deficiency
R250	T449	T431	advmod	specifically,is
R2500	T4000	T3998	pobj	patients,in
R2501	T4001	T4002	advmod	where,reduced
R2502	T4002	T4000	relcl	reduced,patients
R2503	T4003	T4004	compound	MCAD,activity
R2504	T4004	T4002	nsubjpass	activity,reduced
R2505	T4005	T4002	auxpass	is,reduced
R2506	T4006	T4002	prep	to,reduced
R2507	T4007	T4008	quantmod	near,zero
R2508	T4008	T4006	pobj	zero,to
R2509	T4009	T4002	prep	with,reduced
R251	T450	T431	punct	", ",is
R2510	T4010	T4011	compound	C8,CoA
R2511	T4011	T4009	pobj	CoA,with
R2512	T4012	T4011	punct	-,CoA
R2513	T4013	T3983	punct	.,had
R2514	T4015	T4016	nsubj	This,was
R2515	T4017	T4018	advmod	less,so
R2516	T4018	T4016	advmod	so,was
R2517	T4019	T4016	prep	with,was
R2518	T4020	T4021	compound	palmitoyl,CoA
R2519	T4021	T4019	pobj	CoA,with
R252	T451	T431	acomp	responsible,is
R2520	T4022	T4021	punct	-,CoA
R2521	T4023	T4024	punct	(,C16
R2522	T4024	T4016	parataxis	C16,was
R2523	T4025	T4024	punct	:,C16
R2524	T4026	T4024	nummod	0,C16
R2525	T4027	T4024	punct	),C16
R2526	T4028	T4016	punct	.,was
R2527	T4030	T4031	mark	Because,was
R2528	T4031	T4033	advcl	was,represent
R2529	T4032	T4031	expl	there,was
R253	T452	T451	prep	for,responsible
R2530	T4034	T4031	advmod	clearly,was
R2531	T4035	T4036	det	no,antigen
R2532	T4036	T4031	attr	antigen,was
R2533	T4037	T4036	compound	MCAD,antigen
R2534	T4038	T4036	acl	detected,antigen
R2535	T4039	T4038	prep	in,detected
R2536	T4040	T4041	nmod	MCAD,mice
R2537	T4041	T4039	pobj	mice,in
R2538	T4042	T4040	punct	−,MCAD
R2539	T4043	T4040	punct	/,MCAD
R254	T453	T452	pcomp	catalyzing,for
R2540	T4044	T4040	punct	−,MCAD
R2541	T4045	T4033	punct	", ",represent
R2542	T4046	T4047	det	the,activity
R2543	T4047	T4033	nsubj	activity,represent
R2544	T4048	T4047	amod	residual,activity
R2545	T4049	T4047	compound	dehydrogenase,activity
R2546	T4050	T4047	acl	measured,activity
R2547	T4051	T4050	prep	with,measured
R2548	T4052	T4053	det	these,substrates
R2549	T4053	T4051	pobj	substrates,with
R255	T454	T455	det	the,dehydrogenation
R2550	T4054	T4053	nummod	two,substrates
R2551	T4055	T4033	aux	must,represent
R2552	T4056	T4057	det	the,activity
R2553	T4057	T4033	dobj	activity,represent
R2554	T4058	T4057	prep	of,activity
R2555	T4059	T4060	amod	other,dehydrogenases
R2556	T4060	T4058	pobj	dehydrogenases,of
R2557	T4061	T4062	compound	chain,length
R2558	T4062	T4063	npadvmod	length,specific
R2559	T4063	T4060	amod	specific,dehydrogenases
R256	T455	T453	dobj	dehydrogenation,catalyzing
R2560	T4064	T4063	punct	–,specific
R2561	T4065	T4066	compound	acyl,CoA
R2562	T4066	T4060	compound	CoA,dehydrogenases
R2563	T4067	T4066	punct	-,CoA
R2564	T4068	T4033	punct	.,represent
R2565	T4070	T4071	det	A,degree
R2566	T4071	T4073	nsubj	degree,was
R2567	T4072	T4071	amod	high,degree
R2568	T4074	T4071	prep	of,degree
R2569	T4075	T4076	amod	neonatal,mortality
R257	T456	T455	prep	of,dehydrogenation
R2570	T4076	T4074	pobj	mortality,of
R2571	T4077	T4071	prep	in,degree
R2572	T4078	T4079	nmod	MCAD,mice
R2573	T4079	T4077	pobj	mice,in
R2574	T4080	T4078	punct	−,MCAD
R2575	T4081	T4078	punct	/,MCAD
R2576	T4082	T4078	punct	−,MCAD
R2577	T4083	T4084	det	a,observation
R2578	T4084	T4073	attr	observation,was
R2579	T4085	T4084	amod	striking,observation
R258	T457	T458	amod	medium,chain
R2580	T4086	T4073	cc	and,was
R2581	T4087	T4073	conj	appears,was
R2582	T4088	T4089	aux	to,be
R2583	T4089	T4087	xcomp	be,appears
R2584	T4090	T4089	acomp	analogous,be
R2585	T4091	T4090	prep	to,analogous
R2586	T4092	T4093	det	the,patterns
R2587	T4093	T4091	pobj	patterns,to
R2588	T4094	T4093	prep	of,patterns
R2589	T4095	T4096	amod	clinical,episodes
R259	T458	T460	nmod	chain,length
R2590	T4096	T4094	pobj	episodes,of
R2591	T4097	T4096	cc	and,episodes
R2592	T4098	T4096	conj	mortality,episodes
R2593	T4099	T4093	prep	in,patterns
R2594	T4100	T4101	npadvmod	MCAD,deficient
R2595	T4101	T4103	amod	deficient,patients
R2596	T4102	T4101	punct	-,deficient
R2597	T4103	T4099	pobj	patients,in
R2598	T4104	T4073	punct	.,was
R2599	T4106	T4107	nmod	MCAD,mice
R26	T105	T104	punct	−,MCAD
R260	T459	T458	punct	-,chain
R2600	T4107	T4111	nsubj	mice,exhibited
R2601	T4108	T4106	punct	−,MCAD
R2602	T4109	T4106	punct	/,MCAD
R2603	T4110	T4106	punct	−,MCAD
R2604	T4112	T4113	amod	significant,mortality
R2605	T4113	T4111	dobj	mortality,exhibited
R2606	T4114	T4113	amod	neonatal,mortality
R2607	T4115	T4111	prep	with,exhibited
R2608	T4116	T4117	advmod	approximately,60
R2609	T4117	T4118	nummod	60,%
R261	T460	T461	nmod	length,thioesters
R2610	T4118	T4119	nsubj	%,dying
R2611	T4119	T4115	pcomp	dying,with
R2612	T4120	T4118	prep	of,%
R2613	T4121	T4122	det	the,pups
R2614	T4122	T4120	pobj	pups,of
R2615	T4123	T4122	nmod	MCAD,pups
R2616	T4124	T4123	punct	−,MCAD
R2617	T4125	T4123	punct	/,MCAD
R2618	T4126	T4123	punct	−,MCAD
R2619	T4127	T4128	amod	prior,to
R262	T461	T456	pobj	thioesters,of
R2620	T4128	T4119	prep	to,dying
R2621	T4129	T4128	pobj	weaning,to
R2622	T4130	T4119	prep	at,dying
R2623	T4131	T4132	nummod	3,wk
R2624	T4132	T4130	pobj	wk,at
R2625	T4133	T4132	prep	of,wk
R2626	T4134	T4133	pobj	age,of
R2627	T4135	T4111	punct	.,exhibited
R2628	T4137	T4138	amod	Human,patients
R2629	T4138	T4139	nsubj	patients,present
R263	T462	T463	punct	(,C12
R2630	T4140	T4139	prep	with,present
R2631	T4141	T4140	pobj	hypoglycemia,with
R2632	T4142	T4141	punct	", ",hypoglycemia
R2633	T4143	T4141	conj	hypoketonemia,hypoglycemia
R2634	T4144	T4143	punct	", ",hypoketonemia
R2635	T4145	T4143	cc	and,hypoketonemia
R2636	T4146	T4147	amod	nonketotic,aciduria
R2637	T4147	T4143	conj	aciduria,hypoketonemia
R2638	T4148	T4147	amod	organic,aciduria
R2639	T4149	T4147	acl	precipitated,aciduria
R264	T463	T460	parataxis	C12,length
R2640	T4150	T4149	agent	by,precipitated
R2641	T4151	T4150	pobj	fasting,by
R2642	T4152	T4139	punct	", ",present
R2643	T4153	T4154	advmod	most,frequently
R2644	T4154	T4155	advmod	frequently,during
R2645	T4155	T4139	prep	during,present
R2646	T4156	T4157	det	the,mo
R2647	T4157	T4155	pobj	mo,during
R2648	T4158	T4157	amod	first,mo
R2649	T4159	T4157	nummod	24,mo
R265	T464	T463	nmod	C6,C12
R2650	T4160	T4157	prep	in,mo
R2651	T4161	T4160	pobj	life,in
R2652	T4162	T4163	punct	[,1
R2653	T4163	T4139	parataxis	1,present
R2654	T4164	T4163	punct	],1
R2655	T4165	T4139	punct	.,present
R2656	T4167	T4168	nsubj	It,is
R2657	T4169	T4168	acomp	likely,is
R2658	T4170	T4171	mark	that,manifesting
R2659	T4171	T4168	ccomp	manifesting,is
R266	T465	T463	punct	–,C12
R2660	T4172	T4173	amod	neonatal,pups
R2661	T4173	T4171	nsubj	pups,manifesting
R2662	T4174	T4173	nmod	MCAD,pups
R2663	T4175	T4174	punct	−,MCAD
R2664	T4176	T4174	punct	/,MCAD
R2665	T4177	T4174	punct	−,MCAD
R2666	T4178	T4171	aux	are,manifesting
R2667	T4179	T4171	dobj	sensitivity,manifesting
R2668	T4180	T4179	prep	to,sensitivity
R2669	T4181	T4180	pobj	fasting,to
R267	T466	T463	punct	),C12
R2670	T4182	T4171	prep	with,manifesting
R2671	T4183	T4182	pobj	decompensation,with
R2672	T4184	T4183	prep	in,decompensation
R2673	T4185	T4186	det	a,period
R2674	T4186	T4184	pobj	period,in
R2675	T4187	T4186	amod	short,period
R2676	T4188	T4186	prep	of,period
R2677	T4189	T4188	pobj	time,of
R2678	T4190	T4191	mark	if,ingested
R2679	T4191	T4171	advcl	ingested,manifesting
R268	T467	T468	amod	fatty,acid
R2680	T4192	T4193	amod	maternal,milk
R2681	T4193	T4191	nsubjpass	milk,ingested
R2682	T4194	T4191	auxpass	is,ingested
R2683	T4195	T4191	neg	not,ingested
R2684	T4196	T4168	punct	.,is
R2685	T4198	T4199	prep	In,noted
R2686	T4200	T4198	pobj	contrast,In
R2687	T4201	T4199	punct	", ",noted
R2688	T4202	T4203	det	no,mortality
R2689	T4203	T4199	nsubjpass	mortality,noted
R269	T468	T461	compound	acid,thioesters
R2690	T4204	T4199	auxpass	was,noted
R2691	T4205	T4199	prep	in,noted
R2692	T4206	T4207	amod	adult,mice
R2693	T4207	T4205	pobj	mice,in
R2694	T4208	T4207	nmod	MCAD,mice
R2695	T4209	T4208	punct	−,MCAD
R2696	T4210	T4208	punct	/,MCAD
R2697	T4211	T4208	punct	−,MCAD
R2698	T4212	T4213	mark	unless,challenged
R2699	T4213	T4199	advcl	challenged,noted
R27	T106	T104	punct	/,MCAD
R270	T469	T470	punct	[,1
R2700	T4214	T4213	prep	with,challenged
R2701	T4215	T4216	compound	cold,stress
R2702	T4216	T4214	pobj	stress,with
R2703	T4217	T4216	cc	and,stress
R2704	T4218	T4216	conj	fasting,stress
R2705	T4219	T4199	punct	.,noted
R2706	T4221	T4222	prep	Under,were
R2707	T4223	T4224	compound	cold,challenge
R2708	T4224	T4225	compound	challenge,conditions
R2709	T4225	T4221	pobj	conditions,Under
R271	T470	T431	parataxis	1,is
R2710	T4226	T4222	punct	", ",were
R2711	T4227	T4222	advmod	however,were
R2712	T4228	T4222	punct	", ",were
R2713	T4229	T4230	nmod	MCAD,mice
R2714	T4230	T4222	nsubj	mice,were
R2715	T4231	T4229	punct	−,MCAD
R2716	T4232	T4229	punct	/,MCAD
R2717	T4233	T4229	punct	−,MCAD
R2718	T4234	T4222	acomp	unable,were
R2719	T4235	T4236	aux	to,maintain
R272	T471	T470	punct	],1
R2720	T4236	T4234	xcomp	maintain,unable
R2721	T4237	T4238	compound	body,temperature
R2722	T4238	T4236	dobj	temperature,maintain
R2723	T4239	T4222	punct	.,were
R2724	T4241	T4242	amod	Brown,fat
R2725	T4242	T4243	nsubj	fat,is
R2726	T4244	T4245	advmod	predominantly,responsible
R2727	T4245	T4243	acomp	responsible,is
R2728	T4246	T4245	prep	for,responsible
R2729	T4247	T4246	pobj	thermogenesis,for
R273	T472	T431	punct	.,is
R2730	T4248	T4243	cc	and,is
R2731	T4249	T4250	advmod	normally,expresses
R2732	T4250	T4243	conj	expresses,is
R2733	T4251	T4252	amod	high,levels
R2734	T4252	T4250	dobj	levels,expresses
R2735	T4253	T4252	prep	of,levels
R2736	T4254	T4255	compound	Acadm,mRNA
R2737	T4255	T4253	pobj	mRNA,of
R2738	T4256	T4243	punct	.,is
R2739	T4258	T4259	det	The,steatosis
R274	T474	T475	nsubjpass	Acadm,transcribed
R2740	T4259	T4264	nsubj	steatosis,is
R2741	T4260	T4259	amod	microvesicular,steatosis
R2742	T4261	T4260	cc	and,microvesicular
R2743	T4262	T4260	conj	macrovesicular,microvesicular
R2744	T4263	T4259	amod	hepatic,steatosis
R2745	T4265	T4259	acl	seen,steatosis
R2746	T4266	T4265	prep	in,seen
R2747	T4267	T4268	amod	fasted,mice
R2748	T4268	T4266	pobj	mice,in
R2749	T4269	T4268	nmod	MCAD,mice
R275	T476	T475	auxpass	is,transcribed
R2750	T4270	T4269	punct	−,MCAD
R2751	T4271	T4269	punct	/,MCAD
R2752	T4272	T4269	punct	−,MCAD
R2753	T4273	T4264	acomp	consistent,is
R2754	T4274	T4273	prep	with,consistent
R2755	T4275	T4276	det	the,finding
R2756	T4276	T4274	pobj	finding,with
R2757	T4277	T4276	amod	primary,finding
R2758	T4278	T4276	amod	pathological,finding
R2759	T4279	T4276	acl	seen,finding
R276	T477	T475	prep	in,transcribed
R2760	T4280	T4279	prep	in,seen
R2761	T4281	T4282	amod	human,patients
R2762	T4282	T4280	pobj	patients,in
R2763	T4283	T4282	compound	MCAD,patients
R2764	T4284	T4282	prep	with,patients
R2765	T4285	T4286	compound	fasting,stress
R2766	T4286	T4284	pobj	stress,with
R2767	T4287	T4264	punct	.,is
R2768	T4289	T4290	amod	Sporadic,lesions
R2769	T4290	T4292	nsubj	lesions,were
R277	T478	T479	det	the,nucleus
R2770	T4291	T4290	amod	cardiac,lesions
R2771	T4293	T4290	prep	in,lesions
R2772	T4294	T4295	nmod	MCAD,mice
R2773	T4295	T4293	pobj	mice,in
R2774	T4296	T4294	punct	−,MCAD
R2775	T4297	T4294	punct	/,MCAD
R2776	T4298	T4294	punct	−,MCAD
R2777	T4299	T4292	punct	", ",were
R2778	T4300	T4292	advmod	however,were
R2779	T4301	T4292	punct	", ",were
R278	T479	T477	pobj	nucleus,in
R2780	T4302	T4303	det	an,finding
R2781	T4303	T4292	attr	finding,were
R2782	T4304	T4303	amod	interesting,finding
R2783	T4305	T4304	cc	and,interesting
R2784	T4306	T4304	conj	unexpected,interesting
R2785	T4307	T4292	punct	.,were
R2786	T4309	T4310	det	The,cardiomyopathy
R2787	T4310	T4312	nsubjpass	cardiomyopathy,reported
R2788	T4311	T4310	amod	diffuse,cardiomyopathy
R2789	T4312	T4329	ccomp	reported,reported
R279	T480	T475	punct	", ",transcribed
R2790	T4313	T4310	prep	with,cardiomyopathy
R2791	T4314	T4315	amod	multifocal,degeneration
R2792	T4315	T4313	pobj	degeneration,with
R2793	T4316	T4315	compound	myocyte,degeneration
R2794	T4317	T4315	cc	and,degeneration
R2795	T4318	T4315	conj	necrosis,degeneration
R2796	T4319	T4310	acl	observed,cardiomyopathy
R2797	T4320	T4319	prep	in,observed
R2798	T4321	T4322	nmod	MCAD,mice
R2799	T4322	T4320	pobj	mice,in
R28	T107	T104	punct	−,MCAD
R280	T481	T475	conj	translated,transcribed
R2800	T4323	T4321	punct	−,MCAD
R2801	T4324	T4321	punct	/,MCAD
R2802	T4325	T4321	punct	−,MCAD
R2803	T4326	T4312	aux	has,reported
R2804	T4327	T4312	neg	not,reported
R2805	T4328	T4312	auxpass	been,reported
R2806	T4330	T4312	prep	in,reported
R2807	T4331	T4332	amod	human,patients
R2808	T4332	T4330	pobj	patients,in
R2809	T4333	T4332	compound	MCAD,patients
R281	T482	T481	prep	in,translated
R2810	T4334	T4329	punct	", ",reported
R2811	T4335	T4329	advmod	however,reported
R2812	T4336	T4329	punct	", ",reported
R2813	T4337	T4338	amod	cardiac,arrhythmias
R2814	T4338	T4329	nsubjpass	arrhythmias,reported
R2815	T4339	T4338	cc	and,arrhythmias
R2816	T4340	T4338	conj	dysfunction,arrhythmias
R2817	T4341	T4329	aux	have,reported
R2818	T4342	T4329	auxpass	been,reported
R2819	T4343	T4329	prep	in,reported
R282	T483	T484	det	the,cytosol
R2820	T4344	T4345	npadvmod	MCAD,deficient
R2821	T4345	T4347	amod	deficient,patients
R2822	T4346	T4345	punct	-,deficient
R2823	T4347	T4343	pobj	patients,in
R2824	T4348	T4349	punct	[,18
R2825	T4349	T4329	parataxis	18,reported
R2826	T4350	T4349	nummod	17,18
R2827	T4351	T4349	punct	", ",18
R2828	T4352	T4349	punct	],18
R2829	T4353	T4329	punct	.,reported
R283	T484	T482	pobj	cytosol,in
R2830	T4355	T4356	advmod	Interestingly,observed
R2831	T4357	T4356	punct	", ",observed
R2832	T4358	T4356	nsubjpass	cardiomyopathy,observed
R2833	T4359	T4356	aux	has,observed
R2834	T4360	T4356	auxpass	been,observed
R2835	T4361	T4356	prep	in,observed
R2836	T4362	T4363	compound	VLCAD,deficiency
R2837	T4363	T4361	pobj	deficiency,in
R2838	T4364	T4365	punct	[,19
R2839	T4365	T4363	parataxis	19,deficiency
R284	T485	T481	punct	", ",translated
R2840	T4366	T4365	punct	],19
R2841	T4367	T4363	cc	and,deficiency
R2842	T4368	T4369	amod	other,disorders
R2843	T4369	T4363	conj	disorders,deficiency
R2844	T4370	T4369	prep	of,disorders
R2845	T4371	T4372	amod	long,chain
R2846	T4372	T4373	compound	chain,fat
R2847	T4373	T4374	compound	fat,metabolism
R2848	T4374	T4370	pobj	metabolism,of
R2849	T4375	T4376	amod	such,as
R285	T486	T481	cc	and,translated
R2850	T4376	T4369	prep	as,disorders
R2851	T4377	T4378	amod	severe,deficiencies
R2852	T4378	T4376	pobj	deficiencies,as
R2853	T4379	T4380	nmod	CPT,1
R2854	T4380	T4378	nummod	1,deficiencies
R2855	T4381	T4380	punct	-,1
R2856	T4382	T4380	cc	and,1
R2857	T4383	T4384	punct	-,2
R2858	T4384	T4380	conj	2,1
R2859	T4385	T4386	punct	[,1
R286	T487	T481	conj	translocated,translated
R2860	T4386	T4356	parataxis	1,observed
R2861	T4387	T4386	punct	],1
R2862	T4388	T4356	punct	.,observed
R2863	T4390	T4391	advmod	Thus,is
R2864	T4392	T4391	nsubj	it,is
R2865	T4393	T4391	acomp	tempting,is
R2866	T4394	T4395	aux	to,relate
R2867	T4395	T4391	xcomp	relate,is
R2868	T4396	T4397	det	the,problems
R2869	T4397	T4395	dobj	problems,relate
R287	T488	T487	prep	into,translocated
R2870	T4398	T4397	amod	cardiac,problems
R2871	T4399	T4397	prep	in,problems
R2872	T4400	T4401	det	the,mouse
R2873	T4401	T4399	pobj	mouse,in
R2874	T4402	T4395	prep	to,relate
R2875	T4403	T4404	det	the,range
R2876	T4404	T4402	pobj	range,to
R2877	T4405	T4404	amod	apparent,range
R2878	T4406	T4404	amod	broader,range
R2879	T4407	T4404	prep	of,range
R288	T489	T490	det	the,matrix
R2880	T4408	T4409	compound	substrate,utilization
R2881	T4409	T4407	pobj	utilization,of
R2882	T4410	T4409	prep	of,utilization
R2883	T4411	T4412	compound	mouse,MCAD
R2884	T4412	T4410	pobj	MCAD,of
R2885	T4413	T4391	punct	.,is
R2886	T4415	T4416	det	The,lesions
R2887	T4416	T4421	nsubj	lesions,is
R2888	T4417	T4416	amod	inconsistent,lesions
R2889	T4418	T4416	nmod	liver,lesions
R289	T490	T488	pobj	matrix,into
R2890	T4419	T4418	cc	and,liver
R2891	T4420	T4418	conj	cardiac,liver
R2892	T4422	T4416	prep	in,lesions
R2893	T4423	T4424	det	these,mice
R2894	T4424	T4422	pobj	mice,in
R2895	T4425	T4421	acomp	analogous,is
R2896	T4426	T4425	prep	with,analogous
R2897	T4427	T4428	det	the,heterogeneity
R2898	T4428	T4426	pobj	heterogeneity,with
R2899	T4429	T4428	amod	significant,heterogeneity
R29	T108	T89	punct	),developed
R290	T491	T490	amod	mitochondrial,matrix
R2900	T4430	T4431	advmod	inter,intrafamilial
R2901	T4431	T4428	amod	intrafamilial,heterogeneity
R2902	T4432	T4431	punct	-,intrafamilial
R2903	T4433	T4431	cc	and,intrafamilial
R2904	T4434	T4428	amod	phenotypic,heterogeneity
R2905	T4435	T4428	acl	seen,heterogeneity
R2906	T4436	T4435	prep	in,seen
R2907	T4437	T4438	compound	MCAD,deficiency
R2908	T4438	T4436	pobj	deficiency,in
R2909	T4439	T4435	prep	in,seen
R291	T492	T493	punct	[,2
R2910	T4440	T4439	pobj	humans,in
R2911	T4441	T4442	punct	[,20
R2912	T4442	T4421	parataxis	20,is
R2913	T4443	T4442	nummod	1,20
R2914	T4444	T4442	punct	",",20
R2915	T4445	T4442	punct	],20
R2916	T4446	T4421	punct	.,is
R2917	T4448	T4449	prep	In,is
R2918	T4450	T4448	pobj	comparisons,In
R2919	T4451	T4450	prep	with,comparisons
R292	T493	T487	parataxis	2,translocated
R2920	T4452	T4453	poss	our,experiences
R2921	T4453	T4451	pobj	experiences,with
R2922	T4454	T4453	prep	with,experiences
R2923	T4455	T4456	det	the,models
R2924	T4456	T4454	pobj	models,with
R2925	T4457	T4456	amod	other,models
R2926	T4458	T4456	compound	mouse,models
R2927	T4459	T4456	prep	for,models
R2928	T4460	T4461	compound	acyl,CoA
R2929	T4461	T4463	compound	CoA,dehydrogenase
R293	T494	T495	punct	–,4
R2930	T4462	T4461	punct	-,CoA
R2931	T4463	T4464	compound	dehydrogenase,deficiencies
R2932	T4464	T4459	pobj	deficiencies,for
R2933	T4465	T4449	punct	", ",is
R2934	T4466	T4467	det	the,phenotype
R2935	T4467	T4449	nsubj	phenotype,is
R2936	T4468	T4467	amod	overall,phenotype
R2937	T4469	T4467	prep	of,phenotype
R2938	T4470	T4471	nmod	MCAD,mice
R2939	T4471	T4469	pobj	mice,of
R294	T495	T493	prep	4,2
R2940	T4472	T4470	punct	−,MCAD
R2941	T4473	T4470	punct	/,MCAD
R2942	T4474	T4470	punct	−,MCAD
R2943	T4475	T4476	advmod	less,severe
R2944	T4476	T4449	acomp	severe,is
R2945	T4477	T4476	prep	than,severe
R2946	T4478	T4477	pobj	that,than
R2947	T4479	T4478	acl	found,that
R2948	T4480	T4479	prep	in,found
R2949	T4481	T4482	nmod	LCAD,mice
R295	T496	T493	punct	],2
R2950	T4482	T4480	pobj	mice,in
R2951	T4483	T4481	punct	−,LCAD
R2952	T4484	T4481	punct	/,LCAD
R2953	T4485	T4481	punct	−,LCAD
R2954	T4486	T4476	punct	", ",severe
R2955	T4487	T4476	cc	yet,severe
R2956	T4488	T4489	advmod	more,pronounced
R2957	T4489	T4476	conj	pronounced,severe
R2958	T4490	T4489	prep	than,pronounced
R2959	T4491	T4492	det	the,models
R296	T497	T475	punct	.,transcribed
R2960	T4492	T4490	pobj	models,than
R2961	T4493	T4492	nmod	VLCAD,models
R2962	T4494	T4493	punct	−,VLCAD
R2963	T4495	T4493	punct	/,VLCAD
R2964	T4496	T4493	punct	−,VLCAD
R2965	T4497	T4493	cc	or,VLCAD
R2966	T4498	T4493	conj	SCAD,VLCAD
R2967	T4499	T4498	punct	−,SCAD
R2968	T4500	T4498	punct	/,SCAD
R2969	T4501	T4498	punct	−,SCAD
R297	T499	T500	mark	Once,inside
R2970	T4502	T4492	compound	mouse,models
R2971	T4503	T4504	punct	[,22
R2972	T4504	T4449	parataxis	22,is
R2973	T4505	T4504	nummod	16,22
R2974	T4506	T4504	punct	",",22
R2975	T4507	T4504	nummod	21,22
R2976	T4508	T4504	punct	",",22
R2977	T4509	T4504	punct	],22
R2978	T4510	T4449	punct	.,is
R2979	T4512	T4513	nsubj	All,are
R298	T500	T501	advcl	inside,assembled
R2980	T4514	T4512	prep	of,All
R2981	T4515	T4516	det	these,mutants
R2982	T4516	T4514	pobj	mutants,of
R2983	T4517	T4518	npadvmod	cold,intolerant
R2984	T4518	T4513	acomp	intolerant,are
R2985	T4519	T4513	cc	and,are
R2986	T4520	T4513	conj	display,are
R2987	T4521	T4522	amod	varying,degrees
R2988	T4522	T4520	dobj	degrees,display
R2989	T4523	T4522	prep	of,degrees
R299	T502	T503	det	the,matrix
R2990	T4524	T4525	amod	fatty,changes
R2991	T4525	T4523	pobj	changes,of
R2992	T4526	T4520	prep	in,display
R2993	T4527	T4526	pobj	liver,in
R2994	T4528	T4527	punct	", ",liver
R2995	T4529	T4527	conj	heart,liver
R2996	T4530	T4529	punct	", ",heart
R2997	T4531	T4529	cc	and,heart
R2998	T4532	T4529	conj	kidney,heart
R2999	T4533	T4513	punct	.,are
R3	T80	T77	compound	β,oxidation
R30	T109	T89	prep	by,developed
R300	T503	T500	pobj	matrix,inside
R3000	T4535	T4536	nmod	LCAD,mice
R3001	T4536	T4540	nsubj	mice,show
R3002	T4537	T4535	punct	−,LCAD
R3003	T4538	T4535	punct	/,LCAD
R3004	T4539	T4535	punct	−,LCAD
R3005	T4541	T4542	amod	more,deaths
R3006	T4542	T4540	dobj	deaths,show
R3007	T4543	T4542	amod	spontaneous,deaths
R3008	T4544	T4542	cc	and,deaths
R3009	T4545	T4546	amod	gestational,losses
R301	T504	T503	amod	mitochondrial,matrix
R3010	T4546	T4542	conj	losses,deaths
R3011	T4547	T4542	prep	than,deaths
R3012	T4548	T4549	det	the,deficiencies
R3013	T4549	T4547	pobj	deficiencies,than
R3014	T4550	T4549	amod	other,deficiencies
R3015	T4551	T4552	punct	[,21
R3016	T4552	T4540	parataxis	21,show
R3017	T4553	T4552	punct	],21
R3018	T4554	T4540	punct	.,show
R3019	T4556	T4557	det	The,mortality
R302	T505	T501	punct	", ",assembled
R3020	T4557	T4560	nsubj	mortality,is
R3021	T4558	T4557	amod	significant,mortality
R3022	T4559	T4557	amod	neonatal,mortality
R3023	T4561	T4557	prep	in,mortality
R3024	T4562	T4563	nmod	MCAD,mice
R3025	T4563	T4561	pobj	mice,in
R3026	T4564	T4562	punct	−,MCAD
R3027	T4565	T4562	punct	/,MCAD
R3028	T4566	T4562	punct	−,MCAD
R3029	T4567	T4560	acomp	distinctive,is
R303	T506	T507	det	the,monomers
R3030	T4568	T4567	prep	from,distinctive
R3031	T4569	T4570	det	these,models
R3032	T4570	T4568	pobj	models,from
R3033	T4571	T4570	amod	other,models
R3034	T4572	T4570	compound	mouse,models
R3035	T4573	T4560	advcl	suggesting,is
R3036	T4574	T4575	det	a,degree
R3037	T4575	T4573	dobj	degree,suggesting
R3038	T4576	T4575	amod	greater,degree
R3039	T4577	T4575	prep	of,degree
R304	T507	T501	nsubjpass	monomers,assembled
R3040	T4578	T4577	pobj	sensitivity,of
R3041	T4579	T4578	prep	to,sensitivity
R3042	T4580	T4581	compound	fasting,intolerance
R3043	T4581	T4579	pobj	intolerance,to
R3044	T4582	T4560	punct	.,is
R3045	T4584	T4585	det	The,phenotypes
R3046	T4585	T4586	nsubj	phenotypes,are
R3047	T4587	T4585	prep	of,phenotypes
R3048	T4588	T4589	preconj	both,mice
R3049	T4589	T4587	pobj	mice,of
R305	T508	T507	compound	MCAD,monomers
R3050	T4590	T4589	det	the,mice
R3051	T4591	T4589	nmod	VLCAD,mice
R3052	T4592	T4591	punct	−,VLCAD
R3053	T4593	T4591	punct	/,VLCAD
R3054	T4594	T4591	punct	−,VLCAD
R3055	T4595	T4591	cc	and,VLCAD
R3056	T4596	T4591	conj	SCAD,VLCAD
R3057	T4597	T4596	punct	−,SCAD
R3058	T4598	T4596	punct	/,SCAD
R3059	T4599	T4596	punct	−,SCAD
R306	T509	T501	auxpass	are,assembled
R3060	T4600	T4601	advmod	relatively,mild
R3061	T4601	T4586	acomp	mild,are
R3062	T4602	T4603	mark	if,stressed
R3063	T4603	T4586	advcl	stressed,are
R3064	T4604	T4605	det	the,animals
R3065	T4605	T4603	nsubjpass	animals,stressed
R3066	T4606	T4603	auxpass	are,stressed
R3067	T4607	T4603	neg	not,stressed
R3068	T4608	T4603	amod	cold,stressed
R3069	T4609	T4610	punct	[,22
R307	T510	T501	prep	into,assembled
R3070	T4610	T4586	parataxis	22,are
R3071	T4611	T4610	nummod	16,22
R3072	T4612	T4610	punct	",",22
R3073	T4613	T4610	punct	],22
R3074	T4614	T4586	punct	.,are
R3075	T4616	T4617	det	The,mouse
R3076	T4617	T4621	nsubj	mouse,offers
R3077	T4618	T4619	amod	MCAD,deficient
R3078	T4619	T4617	amod	deficient,mouse
R3079	T4620	T4619	punct	-,deficient
R308	T511	T510	pobj	homotetramers,into
R3080	T4622	T4623	amod	new,insights
R3081	T4623	T4621	dobj	insights,offers
R3082	T4624	T4623	prep	into,insights
R3083	T4625	T4626	det	the,pathogenesis
R3084	T4626	T4624	pobj	pathogenesis,into
R3085	T4627	T4626	prep	of,pathogenesis
R3086	T4628	T4629	amod	mitochondrial,deficiencies
R3087	T4629	T4627	pobj	deficiencies,of
R3088	T4630	T4631	compound	β,oxidation
R3089	T4631	T4629	compound	oxidation,deficiencies
R309	T512	T513	aux	to,gain
R3090	T4632	T4631	punct	-,oxidation
R3091	T4633	T4621	cc	and,offers
R3092	T4634	T4635	aux	will,provide
R3093	T4635	T4621	conj	provide,offers
R3094	T4636	T4637	det	a,tool
R3095	T4637	T4635	dobj	tool,provide
R3096	T4638	T4637	amod	robust,tool
R3097	T4639	T4640	aux	to,understand
R3098	T4640	T4637	advcl	understand,tool
R3099	T4641	T4640	advmod	better,understand
R31	T110	T111	compound	gene,targeting
R310	T513	T501	advcl	gain,assembled
R3100	T4642	T4643	det	the,role
R3101	T4643	T4640	dobj	role,understand
R3102	T4644	T4643	prep	of,role
R3103	T4645	T4646	amod	fatty,acids
R3104	T4646	T4644	pobj	acids,of
R3105	T4647	T4643	prep	in,role
R3106	T4648	T4649	amod	other,diseases
R3107	T4649	T4647	pobj	diseases,in
R3108	T4650	T4649	amod	relevant,diseases
R3109	T4651	T4621	punct	.,offers
R311	T514	T515	amod	enzymatic,activity
R3112	T4938	T4937	prep	of,Construction
R3113	T4939	T4940	compound	MCAD,vector
R3114	T4940	T4938	pobj	vector,of
R3115	T4941	T4940	compound	targeting,vector
R3116	T4942	T4937	punct	.,Construction
R3117	T4944	T4945	det	A,cassette
R3118	T4945	T4949	nsubjpass	cassette,subcloned
R3119	T4946	T4947	compound	neomycin,resistance
R312	T515	T513	dobj	activity,gain
R3120	T4947	T4945	compound	resistance,cassette
R3121	T4948	T4945	compound	gene,cassette
R3122	T4950	T4951	punct	[,23
R3123	T4951	T4945	parataxis	23,cassette
R3124	T4952	T4951	punct	],23
R3125	T4953	T4949	auxpass	was,subcloned
R3126	T4954	T4949	prep	into,subcloned
R3127	T4955	T4956	det	the,site
R3128	T4956	T4954	pobj	site,into
R3129	T4957	T4956	compound	SalI,site
R313	T516	T517	punct	[,4
R3130	T4958	T4956	prep	of,site
R3131	T4959	T4958	pobj	pGEM11Zf,of
R3132	T4960	T4961	punct	(,+
R3133	T4961	T4959	punct	+,pGEM11Zf
R3134	T4962	T4961	punct	),+
R3135	T4963	T4949	punct	.,subcloned
R3136	T4965	T4966	det	The,plasmid
R3137	T4966	T4967	nsubjpass	plasmid,digested
R3138	T4968	T4967	auxpass	was,digested
R3139	T4969	T4967	prep	with,digested
R314	T517	T501	parataxis	4,assembled
R3140	T4970	T4969	pobj	EcoRI,with
R3141	T4971	T4967	cc	and,digested
R3142	T4972	T4973	det	the,overhangs
R3143	T4973	T4974	nsubjpass	overhangs,filled
R3144	T4974	T4967	conj	filled,digested
R3145	T4975	T4974	auxpass	were,filled
R3146	T4976	T4974	prep	with,filled
R3147	T4977	T4978	compound	Klenow,enzyme
R3148	T4978	T4976	pobj	enzyme,with
R3149	T4979	T4974	punct	.,filled
R315	T518	T517	punct	],4
R3150	T4981	T4982	amod	Subsequent,ligation
R3151	T4982	T4983	nsubj	ligation,recircularized
R3152	T4984	T4982	prep	of,ligation
R3153	T4985	T4986	det	the,ends
R3154	T4986	T4984	pobj	ends,of
R3155	T4987	T4986	compound	blunt,ends
R3156	T4988	T4989	det	the,vector
R3157	T4989	T4983	dobj	vector,recircularized
R3158	T4990	T4983	cc	and,recircularized
R3159	T4991	T4983	conj	destroyed,recircularized
R316	T519	T501	punct	.,assembled
R3160	T4992	T4993	det	the,site
R3161	T4993	T4991	dobj	site,destroyed
R3162	T4994	T4993	compound	EcoRI,site
R3163	T4995	T4993	prep	within,site
R3164	T4996	T4997	det	the,polylinker
R3165	T4997	T4995	pobj	polylinker,within
R3166	T4998	T4997	prep	of,polylinker
R3167	T4999	T5000	det	the,plasmid
R3168	T5000	T4998	pobj	plasmid,of
R3169	T5001	T5000	nmod	pGEMl1Zf,plasmid
R317	T521	T522	compound	MCAD,activity
R3170	T5002	T5003	punct	(,+
R3171	T5003	T5000	punct	+,plasmid
R3172	T5004	T5003	punct	),+
R3173	T5005	T4983	punct	.,recircularized
R3174	T5007	T5008	advmod	Next,cloned
R3175	T5009	T5008	punct	", ",cloned
R3176	T5010	T5011	det	an,fragment
R3177	T5011	T5008	nsubjpass	fragment,cloned
R3178	T5012	T5013	nummod	8,kb
R3179	T5013	T5011	nmod	kb,fragment
R318	T522	T523	nsubj	activity,is
R3180	T5014	T5013	punct	-,kb
R3181	T5015	T5011	nmod	Acadm,fragment
R3182	T5016	T5011	amod	genomic,fragment
R3183	T5017	T5011	acl	spanning,fragment
R3184	T5018	T5019	nmod	exons,8
R3185	T5019	T5017	dobj	8,spanning
R3186	T5020	T5019	punct	", ",8
R3187	T5021	T5019	conj	9,8
R3188	T5022	T5021	punct	", ",9
R3189	T5023	T5021	cc	and,9
R319	T524	T523	acomp	essential,is
R3190	T5024	T5021	conj	10,9
R3191	T5025	T5017	cc	and,spanning
R3192	T5026	T5017	conj	flanking,spanning
R3193	T5027	T5028	compound	intron,sequences
R3194	T5028	T5026	dobj	sequences,flanking
R3195	T5029	T5011	punct	", ",fragment
R3196	T5030	T5031	advmod	originally,obtained
R3197	T5031	T5011	acl	obtained,fragment
R3198	T5032	T5031	prep	from,obtained
R3199	T5033	T5034	det	a,library
R32	T111	T109	pobj	targeting,by
R320	T525	T524	prep	for,essential
R3200	T5034	T5032	pobj	library,from
R3201	T5035	T5036	nmod	Lambda,FIXII
R3202	T5036	T5034	nmod	FIXII,library
R3203	T5037	T5034	nmod	129Sv,library
R3204	T5038	T5034	nmod	mouse,library
R3205	T5039	T5034	amod	genomic,library
R3206	T5040	T5041	punct	[,25
R3207	T5041	T5034	parataxis	25,library
R3208	T5042	T5041	nummod	24,25
R3209	T5043	T5041	punct	",",25
R321	T526	T527	amod	complete,oxidation
R3210	T5044	T5041	punct	],25
R3211	T5045	T5008	punct	", ",cloned
R3212	T5046	T5008	auxpass	was,cloned
R3213	T5047	T5008	advmod	directionally,cloned
R3214	T5048	T5008	prep	into,cloned
R3215	T5049	T5050	det	the,sites
R3216	T5050	T5048	pobj	sites,into
R3217	T5051	T5050	nmod	NotI,sites
R3218	T5052	T5051	cc	and,NotI
R3219	T5053	T5051	conj	XhoI,NotI
R322	T527	T525	pobj	oxidation,for
R3220	T5054	T5050	prep	of,sites
R3221	T5055	T5054	pobj	pGEM11Zf,of
R3222	T5056	T5057	punct	(,+
R3223	T5057	T5055	punct	+,pGEM11Zf
R3224	T5058	T5057	punct	),+
R3225	T5059	T5008	punct	.,cloned
R3226	T5061	T5062	det	The,vector
R3227	T5062	T5063	nsubjpass	vector,digested
R3228	T5064	T5063	auxpass	was,digested
R3229	T5065	T5063	prep	with,digested
R323	T528	T529	amod	fatty,acid
R3230	T5066	T5065	pobj	BamHI,with
R3231	T5067	T5066	cc	and,BamHI
R3232	T5068	T5066	conj	EcoRI,BamHI
R3233	T5069	T5070	aux	to,remove
R3234	T5070	T5063	advcl	remove,digested
R3235	T5071	T5072	det	a,fragment
R3236	T5072	T5070	dobj	fragment,remove
R3237	T5073	T5074	nummod	1.3,kb
R3238	T5074	T5072	nmod	kb,fragment
R3239	T5075	T5074	punct	-,kb
R324	T529	T527	compound	acid,oxidation
R3240	T5076	T5077	nmod	BamHI,EcoRI
R3241	T5077	T5072	nmod	EcoRI,fragment
R3242	T5078	T5077	punct	/,EcoRI
R3243	T5079	T5072	amod	genomic,fragment
R3244	T5080	T5072	acl	containing,fragment
R3245	T5081	T5080	dobj	exon,containing
R3246	T5082	T5081	nummod	10,exon
R3247	T5083	T5080	cc	and,containing
R3248	T5084	T5080	conj	flanking,containing
R3249	T5085	T5086	compound	intron,sequences
R325	T530	T523	punct	.,is
R3250	T5086	T5084	dobj	sequences,flanking
R3251	T5087	T5063	punct	.,digested
R3252	T5089	T5090	det	The,vector
R3253	T5090	T5092	nsubjpass	vector,purified
R3254	T5091	T5090	amod	digested,vector
R3255	T5093	T5090	punct	", ",vector
R3256	T5094	T5090	prep	without,vector
R3257	T5095	T5096	det	the,fragment
R3258	T5096	T5094	pobj	fragment,without
R3259	T5097	T5098	nummod	1.3,kb
R326	T532	T533	amod	Inherited,deficiency
R3260	T5098	T5096	nmod	kb,fragment
R3261	T5099	T5098	punct	-,kb
R3262	T5100	T5101	nmod	BamHI,EcoRI
R3263	T5101	T5096	nmod	EcoRI,fragment
R3264	T5102	T5101	punct	/,EcoRI
R3265	T5103	T5096	amod	genomic,fragment
R3266	T5104	T5092	punct	", ",purified
R3267	T5105	T5092	auxpass	was,purified
R3268	T5106	T5092	prep	by,purified
R3269	T5107	T5108	compound	gel,purification
R327	T533	T535	nsubj	deficiency,exists
R3270	T5108	T5106	pobj	purification,by
R3271	T5109	T5092	cc	and,purified
R3272	T5110	T5092	conj	recircularized,purified
R3273	T5111	T5110	prep	by,recircularized
R3274	T5112	T5111	pobj	ligation,by
R3275	T5113	T5110	advcl	using,recircularized
R3276	T5114	T5115	nummod	three,oligonucleotides
R3277	T5115	T5113	dobj	oligonucleotides,using
R3278	T5116	T5115	punct	: ,oligonucleotides
R3279	T5117	T5118	nummod	5,AATTGTCGACA
R328	T534	T533	compound	MCAD,deficiency
R3280	T5118	T5115	appos	AATTGTCGACA,oligonucleotides
R3281	T5119	T5117	punct	′,5
R3282	T5120	T5118	punct	-,AATTGTCGACA
R3283	T5121	T5118	punct	-,AATTGTCGACA
R3284	T5122	T5118	nummod	3,AATTGTCGACA
R3285	T5123	T5118	punct	′,AATTGTCGACA
R3286	T5124	T5118	punct	;,AATTGTCGACA
R3287	T5125	T5126	nummod	5,GATCGTCGACA
R3288	T5126	T5118	conj	GATCGTCGACA,AATTGTCGACA
R3289	T5127	T5125	punct	′,5
R329	T536	T535	prep	in,exists
R3290	T5128	T5126	punct	-,GATCGTCGACA
R3291	T5129	T5126	nummod	3,GATCGTCGACA
R3292	T5130	T5126	punct	′,GATCGTCGACA
R3293	T5131	T5126	punct	;,GATCGTCGACA
R3294	T5132	T5126	cc	and,GATCGTCGACA
R3295	T5133	T5134	nummod	5,TCGATGTCGAC
R3296	T5134	T5126	conj	TCGATGTCGAC,GATCGTCGACA
R3297	T5135	T5133	punct	′,5
R3298	T5136	T5134	punct	-,TCGATGTCGAC
R3299	T5137	T5134	punct	-,TCGATGTCGAC
R33	T112	T111	prep	in,targeting
R330	T537	T536	pobj	humans,in
R3300	T5138	T5134	nummod	3,TCGATGTCGAC
R3301	T5139	T5134	punct	′,TCGATGTCGAC
R3302	T5140	T5092	punct	.,purified
R3303	T5142	T5143	det	The,vector
R3304	T5143	T5145	nsubj	vector,contained
R3305	T5144	T5143	amod	recircularized,vector
R3306	T5146	T5143	punct	", ",vector
R3307	T5147	T5143	acl	resulting,vector
R3308	T5148	T5147	prep	from,resulting
R3309	T5149	T5150	det	the,ligation
R331	T538	T535	prep	as,exists
R3310	T5150	T5148	pobj	ligation,from
R3311	T5151	T5150	prep	of,ligation
R3312	T5152	T5153	det	the,arm
R3313	T5153	T5151	pobj	arm,of
R3314	T5154	T5153	amod	long,arm
R3315	T5155	T5150	prep	to,ligation
R3316	T5156	T5157	det	the,arm
R3317	T5157	T5155	pobj	arm,to
R3318	T5158	T5157	amod	short,arm
R3319	T5159	T5150	prep	of,ligation
R332	T539	T540	det	an,disorder
R3320	T5160	T5159	pobj	homology,of
R3321	T5161	T5145	punct	", ",contained
R3322	T5162	T5163	det	a,site
R3323	T5163	T5145	dobj	site,contained
R3324	T5164	T5163	amod	unique,site
R3325	T5165	T5163	compound	SalI,site
R3326	T5166	T5167	advmod	where,deleted
R3327	T5167	T5163	relcl	deleted,site
R3328	T5168	T5169	det	the,region
R3329	T5169	T5167	nsubjpass	region,deleted
R333	T540	T538	pobj	disorder,as
R3330	T5170	T5171	nummod	1.3,kb
R3331	T5171	T5169	nmod	kb,region
R3332	T5172	T5171	punct	-,kb
R3333	T5173	T5169	nmod	exon,region
R3334	T5174	T5173	nummod	10,exon
R3335	T5175	T5167	auxpass	was,deleted
R3336	T5176	T5145	punct	.,contained
R3337	T5281	T5280	prep	of,Generation
R3338	T5282	T5283	npadvmod	MCAD,deficient
R3339	T5283	T5285	amod	deficient,mice
R334	T541	T540	amod	autosomal,disorder
R3340	T5284	T5283	punct	-,deficient
R3341	T5285	T5281	pobj	mice,of
R3342	T5286	T5280	punct	.,Generation
R3343	T5288	T5289	det	The,vector
R3344	T5289	T5292	nsubjpass	vector,linearized
R3345	T5290	T5289	compound	Acadm,vector
R3346	T5291	T5289	compound	insertion,vector
R3347	T5293	T5292	auxpass	was,linearized
R3348	T5294	T5292	prep	by,linearized
R3349	T5295	T5296	compound	SalI,digestion
R335	T542	T540	amod	recessive,disorder
R3350	T5296	T5294	pobj	digestion,by
R3351	T5297	T5296	punct	", ",digestion
R3352	T5298	T5299	det	the,site
R3353	T5299	T5296	appos	site,digestion
R3354	T5300	T5299	prep	of,site
R3355	T5301	T5302	det	the,deletion
R3356	T5302	T5300	pobj	deletion,of
R3357	T5303	T5304	nummod	1.3,kb
R3358	T5304	T5302	nmod	kb,deletion
R3359	T5305	T5304	punct	-,kb
R336	T543	T535	punct	.,exists
R3360	T5306	T5307	amod	genomic,fragment
R3361	T5307	T5302	compound	fragment,deletion
R3362	T5308	T5292	punct	", ",linearized
R3363	T5309	T5292	cc	and,linearized
R3364	T5310	T5292	conj	electroporated,linearized
R3365	T5311	T5310	prep	into,electroporated
R3366	T5312	T5313	nmod	E14,cells
R3367	T5313	T5311	pobj	cells,into
R3368	T5314	T5312	punct	–,E14
R3369	T5315	T5312	nummod	1,E14
R337	T545	T546	compound	MCAD,deficiency
R3370	T5316	T5313	compound	ES,cells
R3371	T5317	T5318	punct	(,gift
R3372	T5318	T5313	parataxis	gift,cells
R3373	T5319	T5318	det	a,gift
R3374	T5320	T5318	amod	kind,gift
R3375	T5321	T5318	prep	from,gift
R3376	T5322	T5323	compound	R.,Kuhn
R3377	T5323	T5321	pobj	Kuhn,from
R3378	T5324	T5318	punct	),gift
R3379	T5325	T5313	punct	", ",cells
R338	T546	T547	nsubjpass	deficiency,described
R3380	T5326	T5313	acl	derived,cells
R3381	T5327	T5326	prep	from,derived
R3382	T5328	T5329	compound	129P2,mice
R3383	T5329	T5327	pobj	mice,from
R3384	T5330	T5292	punct	.,linearized
R3385	T5332	T5333	advmod	Correctly,targeted
R3386	T5333	T5334	amod	targeted,vector
R3387	T5334	T5337	nsubjpass	vector,designed
R3388	T5335	T5334	compound	Acadm,vector
R3389	T5336	T5334	compound	insertion,vector
R339	T548	T547	auxpass	was,described
R3390	T5338	T5337	auxpass	was,designed
R3391	T5339	T5340	aux	to,undergo
R3392	T5340	T5337	advcl	undergo,designed
R3393	T5341	T5342	compound	gap,repair
R3394	T5342	T5340	dobj	repair,undergo
R3395	T5343	T5342	prep	of,repair
R3396	T5344	T5345	det	the,deletion
R3397	T5345	T5343	pobj	deletion,of
R3398	T5346	T5347	nummod	1.3,kb
R3399	T5347	T5345	compound	kb,deletion
R34	T113	T114	amod	embryonic,stem
R340	T549	T547	advmod	first,described
R3400	T5348	T5347	punct	-,kb
R3401	T5349	T5340	prep	upon,undergo
R3402	T5350	T5351	amod	double,stranded
R3403	T5351	T5352	amod	stranded,break
R3404	T5352	T5354	compound	break,repair
R3405	T5353	T5352	punct	–,break
R3406	T5354	T5349	pobj	repair,upon
R3407	T5355	T5356	punct	[,26
R3408	T5356	T5354	parataxis	26,repair
R3409	T5357	T5356	punct	],26
R341	T550	T547	prep	in,described
R3410	T5358	T5354	prep	during,repair
R3411	T5359	T5360	amod	homologous,recombination
R3412	T5360	T5358	pobj	recombination,during
R3413	T5361	T5337	punct	.,designed
R3414	T5363	T5364	compound	Southern,blot
R3415	T5364	T5365	compound	blot,analysis
R3416	T5365	T5366	nsubjpass	analysis,conducted
R3417	T5367	T5366	auxpass	was,conducted
R3418	T5368	T5369	aux	to,confirm
R3419	T5369	T5366	advcl	confirm,conducted
R342	T551	T550	pobj	1982,in
R3420	T5370	T5371	amod	homologous,recombination
R3421	T5371	T5369	dobj	recombination,confirm
R3422	T5372	T5366	punct	.,conducted
R3423	T5374	T5375	amod	Genomic,DNA
R3424	T5375	T5376	nsubjpass	DNA,digested
R3425	T5377	T5375	prep	from,DNA
R3426	T5378	T5379	npadvmod	G418,resistant
R3427	T5379	T5380	amod	resistant,colonies
R3428	T5380	T5377	pobj	colonies,from
R3429	T5381	T5380	compound	ES,colonies
R343	T552	T553	punct	—,1983
R3430	T5382	T5376	auxpass	was,digested
R3431	T5383	T5376	prep	with,digested
R3432	T5384	T5383	pobj	EcoRI,with
R3433	T5385	T5376	punct	", ",digested
R3434	T5386	T5376	conj	electrophoresed,digested
R3435	T5387	T5386	punct	", ",electrophoresed
R3436	T5388	T5386	conj	blotted,electrophoresed
R3437	T5389	T5388	punct	", ",blotted
R3438	T5390	T5388	cc	and,blotted
R3439	T5391	T5388	conj	probed,blotted
R344	T553	T551	prep	1983,1982
R3440	T5392	T5391	prep	with,probed
R3441	T5393	T5394	det	an,probe
R3442	T5394	T5392	pobj	probe,with
R3443	T5395	T5396	nummod	850,bp
R3444	T5396	T5394	compound	bp,probe
R3445	T5397	T5396	punct	-,bp
R3446	T5398	T5399	punct	(,A
R3447	T5399	T5394	parataxis	A,probe
R3448	T5400	T5399	compound	probe,A
R3449	T5401	T5399	punct	),A
R345	T554	T555	punct	[,5
R3450	T5402	T5394	acl	generated,probe
R3451	T5403	T5402	prep	by,generated
R3452	T5404	T5403	pobj	PCR,by
R3453	T5405	T5402	prep	from,generated
R3454	T5406	T5407	compound	Acadm,exon
R3455	T5407	T5405	pobj	exon,from
R3456	T5408	T5407	nummod	10,exon
R3457	T5409	T5405	prep	to,from
R3458	T5410	T5409	pobj	intron,to
R3459	T5411	T5410	nummod	10,intron
R346	T555	T547	parataxis	5,described
R3460	T5412	T5376	punct	.,digested
R3461	T5414	T5415	det	This,fragment
R3462	T5415	T5417	nsubj	fragment,is
R3463	T5416	T5415	compound	DNA,fragment
R3464	T5418	T5417	neg	not,is
R3465	T5419	T5417	acomp	present,is
R3466	T5420	T5417	prep	within,is
R3467	T5421	T5422	compound	Acadm,vector
R3468	T5422	T5420	pobj	vector,within
R3469	T5423	T5422	compound	insertion,vector
R347	T556	T557	punct	–,7
R3470	T5424	T5417	cc	and,is
R3471	T5425	T5426	auxpass	was,expected
R3472	T5426	T5417	conj	expected,is
R3473	T5427	T5428	aux	to,hybridize
R3474	T5428	T5426	xcomp	hybridize,expected
R3475	T5429	T5428	prep	to,hybridize
R3476	T5430	T5431	det	a,fragment
R3477	T5431	T5429	pobj	fragment,to
R3478	T5432	T5433	nummod	13.2,kb
R3479	T5433	T5431	nmod	kb,fragment
R348	T557	T555	prep	7,5
R3480	T5434	T5433	punct	-,kb
R3481	T5435	T5431	amod	genomic,fragment
R3482	T5436	T5431	compound	DNA,fragment
R3483	T5437	T5428	prep	upon,hybridize
R3484	T5438	T5439	amod	homologous,recombination
R3485	T5439	T5437	pobj	recombination,upon
R3486	T5440	T5417	punct	.,is
R3487	T5442	T5443	advmod	Correctly,targeted
R3488	T5443	T5444	amod	targeted,clones
R3489	T5444	T5447	nsubjpass	clones,microinjected
R349	T558	T555	punct	],5
R3490	T5445	T5446	compound	ES,cell
R3491	T5446	T5444	compound	cell,clones
R3492	T5448	T5447	auxpass	were,microinjected
R3493	T5449	T5447	prep	into,microinjected
R3494	T5450	T5451	compound	B6,blastocysts
R3495	T5451	T5449	pobj	blastocysts,into
R3496	T5452	T5453	aux	to,generate
R3497	T5453	T5447	advcl	generate,microinjected
R3498	T5454	T5455	amod	chimeric,mice
R3499	T5455	T5453	dobj	mice,generate
R35	T114	T115	nmod	stem,cells
R350	T559	T547	cc	and,described
R3500	T5456	T5447	punct	.,microinjected
R3501	T5458	T5459	amod	Chimeric,mice
R3502	T5459	T5460	nsubjpass	mice,backcrossed
R3503	T5461	T5460	auxpass	were,backcrossed
R3504	T5462	T5460	advmod	subsequently,backcrossed
R3505	T5463	T5460	prep	to,backcrossed
R3506	T5464	T5465	nmod	B6,mice
R3507	T5465	T5463	pobj	mice,to
R3508	T5466	T5464	cc	and,B6
R3509	T5467	T5464	conj	129P2,B6
R351	T560	T561	aux	has,described
R3510	T5468	T5469	aux	to,produce
R3511	T5469	T5460	advcl	produce,backcrossed
R3512	T5470	T5471	npadvmod	gene,targeted
R3513	T5471	T5473	amod	targeted,mice
R3514	T5472	T5471	punct	-,targeted
R3515	T5473	T5469	dobj	mice,produce
R3516	T5474	T5473	appos	AcadmtmUab1,mice
R3517	T5475	T5474	punct	/,AcadmtmUab1
R3518	T5476	T5474	punct	+,AcadmtmUab1
R3519	T5477	T5478	punct	(,MCAD
R352	T561	T547	conj	described,described
R3520	T5478	T5474	parataxis	MCAD,AcadmtmUab1
R3521	T5479	T5478	punct	+,MCAD
R3522	T5480	T5478	punct	/,MCAD
R3523	T5481	T5478	punct	−,MCAD
R3524	T5482	T5478	punct	),MCAD
R3525	T5483	T5473	cc	and,mice
R3526	T5484	T5485	advmod	eventually,mice
R3527	T5485	T5473	conj	mice,mice
R3528	T5486	T5487	nmod	AcadmtmUab1,tm1Uab
R3529	T5487	T5485	nmod	tm1Uab,mice
R353	T562	T561	auxpass	been,described
R3530	T5488	T5487	punct	/,tm1Uab
R3531	T5489	T5485	nmod	MCAD,mice
R3532	T5490	T5485	punct	−,mice
R3533	T5491	T5485	punct	/,mice
R3534	T5492	T5485	punct	−,mice
R3535	T5493	T5494	punct	(,B6
R3536	T5494	T5485	parataxis	B6,mice
R3537	T5495	T5494	punct	;,B6
R3538	T5496	T5494	nummod	129,B6
R3539	T5497	T5494	punct	),B6
R354	T563	T561	prep	in,described
R3540	T5498	T5460	punct	.,backcrossed
R3541	T5500	T5501	nsubj	Mice,were
R3542	T5502	T5501	acomp	negative,were
R3543	T5503	T5502	prep	for,negative
R3544	T5504	T5505	compound	mouse,pathogens
R3545	T5505	T5503	pobj	pathogens,for
R3546	T5506	T5501	prep	based,were
R3547	T5507	T5506	prep	on,based
R3548	T5508	T5509	amod	serological,assays
R3549	T5509	T5507	pobj	assays,on
R355	T564	T565	amod	numerous,patients
R3550	T5510	T5509	prep	for,assays
R3551	T5511	T5512	nummod	ten,viruses
R3552	T5512	T5510	pobj	viruses,for
R3553	T5513	T5512	amod	different,viruses
R3554	T5514	T5509	punct	", ",assays
R3555	T5515	T5516	amod	aerobic,cultures
R3556	T5516	T5509	conj	cultures,assays
R3557	T5517	T5516	amod	bacterial,cultures
R3558	T5518	T5516	prep	of,cultures
R3559	T5519	T5518	pobj	nasopharynx,of
R356	T565	T563	pobj	patients,in
R3560	T5520	T5519	cc	and,nasopharynx
R3561	T5521	T5519	conj	cecum,nasopharynx
R3562	T5522	T5516	punct	", ",cultures
R3563	T5523	T5516	conj	examinations,cultures
R3564	T5524	T5523	prep	for,examinations
R3565	T5525	T5526	nmod	endo,ectoparasites
R3566	T5526	T5524	pobj	ectoparasites,for
R3567	T5527	T5526	punct	-,ectoparasites
R3568	T5528	T5526	cc	and,ectoparasites
R3569	T5529	T5523	punct	", ",examinations
R357	T566	T567	punct	[,1
R3570	T5530	T5523	cc	and,examinations
R3571	T5531	T5523	conj	histopathology,examinations
R3572	T5532	T5531	prep	of,histopathology
R3573	T5533	T5534	det	all,organs
R3574	T5534	T5532	pobj	organs,of
R3575	T5535	T5534	amod	major,organs
R3576	T5536	T5501	punct	.,were
R3577	T5679	T5680	compound	RNA,analysis
R3578	T5681	T5680	punct	.,analysis
R3579	T5683	T5684	amod	Total,RNA
R358	T567	T561	parataxis	1,described
R3580	T5684	T5685	nsubjpass	RNA,isolated
R3581	T5686	T5685	auxpass	was,isolated
R3582	T5687	T5685	prep	from,isolated
R3583	T5688	T5689	det	the,heart
R3584	T5689	T5687	pobj	heart,from
R3585	T5690	T5689	prep	of,heart
R3586	T5691	T5692	nummod	30,day
R3587	T5692	T5693	npadvmod	day,old
R3588	T5693	T5694	amod	old,mice
R3589	T5694	T5690	pobj	mice,of
R359	T568	T567	punct	",",1
R3590	T5695	T5694	nmod	MCAD,mice
R3591	T5696	T5695	punct	+,MCAD
R3592	T5697	T5695	punct	/,MCAD
R3593	T5698	T5695	punct	+,MCAD
R3594	T5699	T5695	punct	", ",MCAD
R3595	T5700	T5695	conj	MCAD,MCAD
R3596	T5701	T5700	punct	+,MCAD
R3597	T5702	T5700	punct	/,MCAD
R3598	T5703	T5700	punct	−,MCAD
R3599	T5704	T5700	punct	", ",MCAD
R36	T115	T112	pobj	cells,in
R360	T569	T567	appos	8,1
R3600	T5705	T5700	cc	and,MCAD
R3601	T5706	T5700	conj	MCAD,MCAD
R3602	T5707	T5706	punct	−,MCAD
R3603	T5708	T5706	punct	/,MCAD
R3604	T5709	T5706	punct	−,MCAD
R3605	T5710	T5685	prep	by,isolated
R3606	T5711	T5712	amod	standard,techniques
R3607	T5712	T5710	pobj	techniques,by
R3608	T5713	T5685	advcl	using,isolated
R3609	T5714	T5715	compound	guanidinium,method
R361	T570	T571	punct	–,11
R3610	T5715	T5713	dobj	method,using
R3611	T5716	T5715	compound	thiocyanate,method
R3612	T5717	T5718	punct	[,27
R3613	T5718	T5685	parataxis	27,isolated
R3614	T5719	T5718	punct	],27
R3615	T5720	T5685	punct	.,isolated
R3616	T5722	T5723	amod	Reverse,transcription
R3617	T5723	T5724	nsubjpass	transcription,performed
R3618	T5725	T5724	auxpass	was,performed
R3619	T5726	T5724	advcl	using,performed
R362	T571	T569	prep	11,8
R3620	T5727	T5728	amod	random,oligonucleotides
R3621	T5728	T5726	dobj	oligonucleotides,using
R3622	T5729	T5730	mark	as,recommended
R3623	T5730	T5724	advcl	recommended,performed
R3624	T5731	T5730	agent	by,recommended
R3625	T5732	T5733	det	the,manufacturer
R3626	T5733	T5731	pobj	manufacturer,by
R3627	T5734	T5735	punct	(,Clontech
R3628	T5735	T5733	parataxis	Clontech,manufacturer
R3629	T5736	T5735	punct	", ",Clontech
R363	T572	T567	punct	],1
R3630	T5737	T5738	compound	Mountain,View
R3631	T5738	T5735	npadvmod	View,Clontech
R3632	T5739	T5735	punct	", ",Clontech
R3633	T5740	T5735	npadvmod	California,Clontech
R3634	T5741	T5735	punct	", ",Clontech
R3635	T5742	T5743	compound	United,States
R3636	T5743	T5735	npadvmod	States,Clontech
R3637	T5744	T5735	punct	),Clontech
R3638	T5745	T5724	punct	.,performed
R3639	T5747	T5748	nsubjpass	PCR,performed
R364	T573	T547	punct	.,described
R3640	T5749	T5748	auxpass	was,performed
R3641	T5750	T5748	advmod	subsequently,performed
R3642	T5751	T5748	advcl	using,performed
R3643	T5752	T5751	dobj	oligonucleotides,using
R3644	T5753	T5752	amod	specific,oligonucleotides
R3645	T5754	T5753	prep	to,specific
R3646	T5755	T5754	pobj	exon,to
R3647	T5756	T5755	nummod	7,exon
R3648	T5757	T5755	cc	and,exon
R3649	T5758	T5755	conj	exon,exon
R365	T575	T576	det	The,frequency
R3650	T5759	T5758	nummod	11,exon
R3651	T5760	T5755	prep	of,exon
R3652	T5761	T5760	pobj	Acadm,of
R3653	T5762	T5748	punct	.,performed
R3654	T5764	T5765	compound	PCR,amplifications
R3655	T5765	T5766	nsubjpass	amplifications,performed
R3656	T5767	T5766	auxpass	were,performed
R3657	T5768	T5769	mark	as,described
R3658	T5769	T5766	advcl	described,performed
R3659	T5770	T5769	advmod	above,described
R366	T576	T578	nsubjpass	frequency,estimated
R3660	T5771	T5766	punct	.,performed
R3661	T5773	T5774	compound	PCR,fragments
R3662	T5774	T5775	nsubjpass	fragments,sequenced
R3663	T5776	T5775	auxpass	were,sequenced
R3664	T5777	T5775	advmod	subsequently,sequenced
R3665	T5778	T5775	prep	after,sequenced
R3666	T5779	T5778	pcomp	subcloning,after
R3667	T5780	T5779	prep	into,subcloning
R3668	T5781	T5782	compound	pGEM,T
R3669	T5782	T5784	compound	T,vector
R367	T577	T576	compound	carrier,frequency
R3670	T5783	T5782	punct	-,T
R3671	T5784	T5780	pobj	vector,into
R3672	T5785	T5784	compound	Easy,vector
R3673	T5786	T5787	punct	(,Promega
R3674	T5787	T5784	parataxis	Promega,vector
R3675	T5788	T5787	punct	", ",Promega
R3676	T5789	T5787	npadvmod	Madison,Promega
R3677	T5790	T5787	punct	", ",Promega
R3678	T5791	T5787	npadvmod	Wisconsin,Promega
R3679	T5792	T5787	punct	", ",Promega
R368	T579	T576	prep	in,frequency
R3680	T5793	T5794	compound	United,States
R3681	T5794	T5787	npadvmod	States,Promega
R3682	T5795	T5787	punct	),Promega
R3683	T5796	T5775	punct	.,sequenced
R3684	T5798	T5799	prep	In,performed
R3685	T5800	T5798	pobj	order,In
R3686	T5801	T5802	aux	to,determine
R3687	T5802	T5800	acl	determine,order
R3688	T5803	T5804	det	the,extent
R3689	T5804	T5802	dobj	extent,determine
R369	T580	T581	det	the,population
R3690	T5805	T5804	prep	of,extent
R3691	T5806	T5807	compound	Acadm,mRNA
R3692	T5807	T5805	pobj	mRNA,of
R3693	T5808	T5807	acl	expressed,mRNA
R3694	T5809	T5808	prep	from,expressed
R3695	T5810	T5811	det	the,mice
R3696	T5811	T5809	pobj	mice,from
R3697	T5812	T5811	nmod	MCAD,mice
R3698	T5813	T5812	punct	−,MCAD
R3699	T5814	T5812	punct	/,MCAD
R37	T116	T114	punct	(,stem
R370	T581	T579	pobj	population,in
R3700	T5815	T5812	punct	−,MCAD
R3701	T5816	T5799	punct	", ",performed
R3702	T5817	T5818	compound	northern,blot
R3703	T5818	T5819	compound	blot,analysis
R3704	T5819	T5799	nsubjpass	analysis,performed
R3705	T5820	T5799	auxpass	was,performed
R3706	T5821	T5799	punct	.,performed
R3707	T5823	T5824	amod	Total,RNA
R3708	T5824	T5825	nsubjpass	RNA,isolated
R3709	T5826	T5825	auxpass	was,isolated
R371	T582	T581	amod	Caucasian,population
R3710	T5827	T5825	prep	from,isolated
R3711	T5828	T5827	pobj	heart,from
R3712	T5829	T5828	punct	", ",heart
R3713	T5830	T5828	conj	liver,heart
R3714	T5831	T5830	punct	", ",liver
R3715	T5832	T5833	amod	brown,fat
R3716	T5833	T5830	conj	fat,liver
R3717	T5834	T5833	punct	", ",fat
R3718	T5835	T5833	conj	brain,fat
R3719	T5836	T5835	punct	", ",brain
R372	T583	T578	aux	has,estimated
R3720	T5837	T5835	conj	kidney,brain
R3721	T5838	T5837	punct	", ",kidney
R3722	T5839	T5840	amod	skeletal,muscle
R3723	T5840	T5837	conj	muscle,kidney
R3724	T5841	T5840	punct	", ",muscle
R3725	T5842	T5843	amod	white,fat
R3726	T5843	T5840	conj	fat,muscle
R3727	T5844	T5843	punct	", ",fat
R3728	T5845	T5843	cc	and,fat
R3729	T5846	T5843	conj	testes,fat
R373	T584	T578	auxpass	been,estimated
R3730	T5847	T5828	prep	of,heart
R3731	T5848	T5849	nummod	3,mo
R3732	T5849	T5851	npadvmod	mo,old
R3733	T5850	T5849	punct	-,mo
R3734	T5851	T5853	amod	old,mice
R3735	T5852	T5851	punct	-,old
R3736	T5853	T5847	pobj	mice,of
R3737	T5854	T5853	nmod	MCAD,mice
R3738	T5855	T5854	punct	+,MCAD
R3739	T5856	T5854	punct	/,MCAD
R374	T585	T586	aux	to,be
R3740	T5857	T5854	punct	+,MCAD
R3741	T5858	T5854	cc	and,MCAD
R3742	T5859	T5854	conj	MCAD,MCAD
R3743	T5860	T5859	punct	−,MCAD
R3744	T5861	T5859	punct	/,MCAD
R3745	T5862	T5859	punct	−,MCAD
R3746	T5863	T5825	advcl	using,isolated
R3747	T5864	T5865	det	the,Kit
R3748	T5865	T5863	dobj	Kit,using
R3749	T5866	T5865	compound	Ultraspec,Kit
R375	T586	T578	xcomp	be,estimated
R3750	T5867	T5868	compound	RNA,Isolation
R3751	T5868	T5865	compound	Isolation,Kit
R3752	T5869	T5870	punct	(,Laboratories
R3753	T5870	T5865	parataxis	Laboratories,Kit
R3754	T5871	T5870	compound	BIOTEX,Laboratories
R3755	T5872	T5870	punct	", ",Laboratories
R3756	T5873	T5870	amod	Inc.,Laboratories
R3757	T5874	T5870	punct	", ",Laboratories
R3758	T5875	T5870	npadvmod	Houston,Laboratories
R3759	T5876	T5870	punct	", ",Laboratories
R376	T587	T588	quantmod	between,80
R3760	T5877	T5870	npadvmod	Texas,Laboratories
R3761	T5878	T5870	punct	", ",Laboratories
R3762	T5879	T5880	compound	United,States
R3763	T5880	T5870	npadvmod	States,Laboratories
R3764	T5881	T5870	punct	),Laboratories
R3765	T5882	T5863	prep	as,using
R3766	T5883	T5882	prep	per,as
R3767	T5884	T5885	poss	manufacturer,protocol
R3768	T5885	T5883	pobj	protocol,per
R3769	T5886	T5884	case	's,manufacturer
R377	T588	T586	attr	80,be
R3770	T5887	T5825	punct	.,isolated
R3771	T5889	T5890	nummod	Ten,μg
R3772	T5890	T5891	nsubjpass	μg,loaded
R3773	T5892	T5890	prep	of,μg
R3774	T5893	T5894	amod	total,RNA
R3775	T5894	T5892	pobj	RNA,of
R3776	T5895	T5890	prep	from,μg
R3777	T5896	T5897	det	each,sample
R3778	T5897	T5895	pobj	sample,from
R3779	T5898	T5891	auxpass	was,loaded
R378	T589	T588	quantmod	1,80
R3780	T5899	T5891	prep	onto,loaded
R3781	T5900	T5901	det	a,gel
R3782	T5901	T5899	pobj	gel,onto
R3783	T5902	T5903	nummod	0.8,%
R3784	T5903	T5904	compound	%,formaldehyde
R3785	T5904	T5901	compound	formaldehyde,gel
R3786	T5905	T5904	compound	agarose,formaldehyde
R3787	T5906	T5904	punct	-,formaldehyde
R3788	T5907	T5891	punct	", ",loaded
R3789	T5908	T5891	conj	transferred,loaded
R379	T590	T588	quantmod	in,80
R3790	T5909	T5908	prep	to,transferred
R3791	T5910	T5911	compound	nitrocellulose,filter
R3792	T5911	T5909	pobj	filter,to
R3793	T5912	T5913	punct	(,Corp.
R3794	T5913	T5911	parataxis	Corp.,filter
R3795	T5914	T5915	compound	Hybond,N
R3796	T5915	T5913	dep	N,Corp.
R3797	T5916	T5913	punct	;,Corp.
R3798	T5917	T5913	compound	GE,Corp.
R3799	T5918	T5913	compound	Healthcare,Corp.
R38	T117	T114	appos	ES,stem
R380	T591	T588	quantmod	50,80
R3800	T5919	T5913	compound	Amersham,Corp.
R3801	T5920	T5913	compound	Biosciences,Corp.
R3802	T5921	T5913	punct	", ",Corp.
R3803	T5922	T5913	npadvmod	Piscataway,Corp.
R3804	T5923	T5913	punct	", ",Corp.
R3805	T5924	T5925	compound	New,Jersey
R3806	T5925	T5913	npadvmod	Jersey,Corp.
R3807	T5926	T5913	punct	", ",Corp.
R3808	T5927	T5928	compound	United,States
R3809	T5928	T5913	npadvmod	States,Corp.
R381	T592	T588	quantmod	to,80
R3810	T5929	T5913	punct	),Corp.
R3811	T5930	T5908	punct	", ",transferred
R3812	T5931	T5908	cc	and,transferred
R3813	T5932	T5908	conj	hybridized,transferred
R3814	T5933	T5932	prep	with,hybridized
R3815	T5934	T5935	npadvmod	32P,radiolabeled
R3816	T5935	T5937	amod	radiolabeled,probe
R3817	T5936	T5935	punct	-,radiolabeled
R3818	T5937	T5933	pobj	probe,with
R3819	T5938	T5939	amod	full,length
R382	T593	T586	prep	with,be
R3820	T5939	T5937	compound	length,probe
R3821	T5940	T5939	punct	-,length
R3822	T5941	T5937	compound	mouse,probe
R3823	T5942	T5937	compound	Acadm,probe
R3824	T5943	T5937	compound	cDNA,probe
R3825	T5944	T5932	advcl	using,hybridized
R3826	T5945	T5946	amod	standard,procedures
R3827	T5946	T5944	dobj	procedures,using
R3828	T5947	T5948	punct	[,28
R3829	T5948	T5932	parataxis	28,hybridized
R383	T594	T595	det	an,incidence
R3830	T5949	T5948	punct	],28
R3831	T5950	T5891	punct	.,loaded
R3832	T5952	T5953	nsubjpass	Hybridizations,performed
R3833	T5954	T5953	auxpass	were,performed
R3834	T5955	T5953	prep	under,performed
R3835	T5956	T5957	advmod	highly,stringent
R3836	T5957	T5958	amod	stringent,conditions
R3837	T5958	T5955	pobj	conditions,under
R3838	T5959	T5960	punct	(,°C
R3839	T5960	T5955	parataxis	°C,under
R384	T595	T593	pobj	incidence,with
R3840	T5961	T5960	nummod	42,°C
R3841	T5962	T5960	prep	in,°C
R3842	T5963	T5964	nummod	2,SSC
R3843	T5964	T5962	pobj	SSC,in
R3844	T5965	T5963	punct	×,2
R3845	T5966	T5960	punct	", ",°C
R3846	T5967	T5968	nummod	50,%
R3847	T5968	T5969	compound	%,formamide
R3848	T5969	T5960	conj	formamide,°C
R3849	T5970	T5969	punct	", ",formamide
R385	T596	T595	prep	of,incidence
R3850	T5971	T5972	nummod	10,%
R3851	T5972	T5973	compound	%,sulfate
R3852	T5973	T5969	conj	sulfate,formamide
R3853	T5974	T5973	compound	dextran,sulfate
R3854	T5975	T5973	punct	", ",sulfate
R3855	T5976	T5977	nummod	5,reagent
R3856	T5977	T5973	conj	reagent,sulfate
R3857	T5978	T5976	punct	×,5
R3858	T5979	T5977	poss	Denhardt,reagent
R3859	T5980	T5979	case	's,Denhardt
R386	T597	T598	amod	clinical,disease
R3860	T5981	T5977	punct	", ",reagent
R3861	T5982	T5983	nummod	1,%
R3862	T5983	T5984	compound	%,SDS
R3863	T5984	T5977	conj	SDS,reagent
R3864	T5985	T5984	punct	", ",SDS
R3865	T5986	T5984	cc	and,SDS
R3866	T5987	T5988	compound	salmon,sperm
R3867	T5988	T5989	compound	sperm,DNA
R3868	T5989	T5984	conj	DNA,SDS
R3869	T5990	T5960	punct	),°C
R387	T598	T596	pobj	disease,of
R3870	T5991	T5953	prep	for,performed
R3871	T5992	T5993	nummod	18,h
R3872	T5993	T5991	pobj	h,for
R3873	T5994	T5953	punct	.,performed
R3874	T5996	T5997	det	The,filter
R3875	T5997	T5999	nsubj	filter,washed
R3876	T5998	T5997	amod	hybridized,filter
R3877	T6000	T5999	aux	was,washed
R3878	T6001	T6002	nummod	two,times
R3879	T6002	T5999	npadvmod	times,washed
R388	T599	T598	acl	expected,disease
R3880	T6003	T5999	prep	in,washed
R3881	T6004	T6005	nummod	4,SSC
R3882	T6005	T6003	pobj	SSC,in
R3883	T6006	T6004	punct	×,4
R3884	T6007	T6005	punct	", ",SSC
R3885	T6008	T6009	nummod	0.1,%
R3886	T6009	T6010	compound	%,SDS
R3887	T6010	T6005	appos	SDS,SSC
R3888	T6011	T5999	cc	and,washed
R3889	T6012	T6013	nummod	two,times
R389	T600	T599	prep	at,expected
R3890	T6013	T5999	conj	times,washed
R3891	T6014	T6013	prep	in,times
R3892	T6015	T6016	nummod	2,SSC
R3893	T6016	T6014	pobj	SSC,in
R3894	T6017	T6015	punct	×,2
R3895	T6018	T6016	punct	;,SSC
R3896	T6019	T6020	nummod	0.1,%
R3897	T6020	T6021	compound	%,SDS
R3898	T6021	T6016	appos	SDS,SSC
R3899	T6022	T5999	prep	at,washed
R39	T118	T115	punct	),cells
R390	T601	T602	quantmod	around,1
R3900	T6023	T6024	nummod	55,°C
R3901	T6024	T6022	pobj	°C,at
R3902	T6025	T5999	prep	for,washed
R3903	T6026	T6027	nummod	1,h
R3904	T6027	T6025	pobj	h,for
R3905	T6028	T5999	punct	.,washed
R3906	T6030	T6031	det	The,filter
R3907	T6031	T6032	nsubjpass	filter,exposed
R3908	T6033	T6032	auxpass	was,exposed
R3909	T6034	T6032	prep	to,exposed
R391	T602	T603	nummod	1,"15,000"
R3910	T6035	T6036	amod	autoradiographic,film
R3911	T6036	T6034	pobj	film,to
R3912	T6037	T6038	punct	(,Biosciences
R3913	T6038	T6036	parataxis	Biosciences,film
R3914	T6039	T6040	compound	Hyperfilm,MP
R3915	T6040	T6038	dep	MP,Biosciences
R3916	T6041	T6038	punct	;,Biosciences
R3917	T6042	T6038	compound	GE,Biosciences
R3918	T6043	T6038	compound	Healthcare,Biosciences
R3919	T6044	T6038	compound	Amersham,Biosciences
R392	T603	T600	pobj	"15,000",at
R3920	T6045	T6038	punct	", ",Biosciences
R3921	T6046	T6038	npadvmod	Piscataway,Biosciences
R3922	T6047	T6038	punct	", ",Biosciences
R3923	T6048	T6049	compound	New,Jersey
R3924	T6049	T6038	npadvmod	Jersey,Biosciences
R3925	T6050	T6038	punct	", ",Biosciences
R3926	T6051	T6052	compound	United,States
R3927	T6052	T6038	npadvmod	States,Biosciences
R3928	T6053	T6038	punct	),Biosciences
R3929	T6054	T6032	punct	.,exposed
R393	T604	T602	quantmod	in,1
R3930	T6056	T6057	amod	Replicate,gels
R3931	T6057	T6061	nsubjpass	gels,stained
R3932	T6058	T6059	compound	agarose,formaldehyde
R3933	T6059	T6057	compound	formaldehyde,gels
R3934	T6060	T6059	punct	-,formaldehyde
R3935	T6062	T6061	auxpass	were,stained
R3936	T6063	T6061	prep	by,stained
R3937	T6064	T6065	compound	ethidium,bromide
R3938	T6065	T6063	pobj	bromide,by
R3939	T6066	T6067	aux	to,verify
R394	T605	T606	punct	[,12
R3940	T6067	T6061	advcl	verify,stained
R3941	T6068	T6069	amod	equal,loading
R3942	T6069	T6067	dobj	loading,verify
R3943	T6070	T6069	compound	RNA,loading
R3944	T6071	T6061	punct	.,stained
R3945	T6221	T6222	compound	Immunoblot,analysis
R3946	T6223	T6222	prep	of,analysis
R3947	T6224	T6225	compound	MCAD,protein
R3948	T6225	T6223	pobj	protein,of
R3949	T6226	T6222	punct	.,analysis
R395	T606	T586	parataxis	12,be
R3950	T6228	T6229	aux	To,evaluate
R3951	T6229	T6230	advcl	evaluate,frozen
R3952	T6231	T6232	det	the,quantity
R3953	T6232	T6229	dobj	quantity,evaluate
R3954	T6233	T6232	prep	of,quantity
R3955	T6234	T6235	compound	MCAD,protein
R3956	T6235	T6233	pobj	protein,of
R3957	T6236	T6229	prep	in,evaluate
R3958	T6237	T6238	compound	mouse,tissue
R3959	T6238	T6236	pobj	tissue,in
R396	T607	T606	nummod	1,12
R3960	T6239	T6230	punct	", ",frozen
R3961	T6240	T6241	compound	liver,samples
R3962	T6241	T6230	nsubjpass	samples,frozen
R3963	T6242	T6241	prep	from,samples
R3964	T6243	T6244	quantmod	6,8
R3965	T6244	T6246	nummod	8,wk
R3966	T6245	T6244	punct	–,8
R3967	T6246	T6248	npadvmod	wk,old
R3968	T6247	T6246	punct	-,wk
R3969	T6248	T6250	amod	old,mice
R397	T608	T606	punct	",",12
R3970	T6249	T6248	punct	-,old
R3971	T6250	T6242	pobj	mice,from
R3972	T6251	T6250	nmod	MCAD,mice
R3973	T6252	T6251	punct	+,MCAD
R3974	T6253	T6251	punct	/,MCAD
R3975	T6254	T6251	punct	+,MCAD
R3976	T6255	T6256	punct	(,1
R3977	T6256	T6251	parataxis	1,MCAD
R3978	T6257	T6256	nsubj	n,1
R3979	T6258	T6256	punct	=,1
R398	T609	T606	nummod	9,12
R3980	T6259	T6256	punct	),1
R3981	T6260	T6251	cc	and,MCAD
R3982	T6261	T6251	conj	MCAD,MCAD
R3983	T6262	T6261	punct	−,MCAD
R3984	T6263	T6261	punct	/,MCAD
R3985	T6264	T6261	punct	−,MCAD
R3986	T6265	T6266	punct	(,3
R3987	T6266	T6261	parataxis	3,MCAD
R3988	T6267	T6266	nsubj	n,3
R3989	T6268	T6266	punct	=,3
R399	T610	T606	punct	",",12
R3990	T6269	T6266	punct	),3
R3991	T6270	T6230	auxpass	were,frozen
R3992	T6271	T6230	advmod	immediately,frozen
R3993	T6272	T6230	prep	in,frozen
R3994	T6273	T6274	amod	liquid,nitrogen
R3995	T6274	T6272	pobj	nitrogen,in
R3996	T6275	T6230	cc	and,frozen
R3997	T6276	T6230	conj	stored,frozen
R3998	T6277	T6276	prep	at,stored
R3999	T6278	T6279	punct	−,80
R4	T81	T77	punct	-,oxidation
R40	T119	T89	punct	.,developed
R400	T611	T606	punct	],12
R4000	T6279	T6280	nummod	80,°C
R4001	T6280	T6277	pobj	°C,at
R4002	T6281	T6230	punct	.,frozen
R4003	T6283	T6284	prep	For,homogenized
R4004	T6285	T6283	pobj	analysis,For
R4005	T6286	T6284	punct	", ",homogenized
R4006	T6287	T6284	nsubjpass	tissue,homogenized
R4007	T6288	T6284	auxpass	was,homogenized
R4008	T6289	T6284	cc	and,homogenized
R4009	T6290	T6284	conj	lysed,homogenized
R401	T612	T578	punct	.,estimated
R4010	T6291	T6290	prep	in,lysed
R4011	T6292	T6293	compound	RIPA,buffer
R4012	T6293	T6291	pobj	buffer,in
R4013	T6294	T6295	punct	(,PBS
R4014	T6295	T6293	parataxis	PBS,buffer
R4015	T6296	T6295	nummod	1,PBS
R4016	T6297	T6296	punct	×,1
R4017	T6298	T6295	punct	", ",PBS
R4018	T6299	T6300	nummod	1,%
R4019	T6300	T6301	compound	%,P
R402	T614	T615	npadvmod	MCAD,deficient
R4020	T6301	T6295	conj	P,PBS
R4021	T6302	T6301	compound	Nonidet,P
R4022	T6303	T6301	punct	-,P
R4023	T6304	T6301	nummod	40,P
R4024	T6305	T6301	punct	", ",P
R4025	T6306	T6307	nummod	0.5,%
R4026	T6307	T6308	compound	%,deoxycholate
R4027	T6308	T6301	conj	deoxycholate,P
R4028	T6309	T6308	compound	sodium,deoxycholate
R4029	T6310	T6308	punct	", ",deoxycholate
R403	T615	T617	amod	deficient,patients
R4030	T6311	T6308	cc	and,deoxycholate
R4031	T6312	T6313	nummod	1,%
R4032	T6313	T6314	compound	%,SDS
R4033	T6314	T6308	conj	SDS,deoxycholate
R4034	T6315	T6295	punct	),PBS
R4035	T6316	T6290	prep	with,lysed
R4036	T6317	T6318	nummod	10,%
R4037	T6318	T6319	compound	%,glycerol
R4038	T6319	T6316	pobj	glycerol,with
R4039	T6320	T6319	cc	and,glycerol
R404	T616	T615	punct	-,deficient
R4040	T6321	T6322	amod	Complete,Inhibitor
R4041	T6322	T6319	conj	Inhibitor,glycerol
R4042	T6323	T6322	compound	Protease,Inhibitor
R4043	T6324	T6322	punct	", ",Inhibitor
R4044	T6325	T6326	punct	(,Corporation
R4045	T6326	T6322	conj	Corporation,Inhibitor
R4046	T6327	T6326	compound	Roche,Corporation
R4047	T6328	T6326	compound	Diagnostics,Corporation
R4048	T6329	T6326	punct	", ",Corporation
R4049	T6330	T6326	npadvmod	Indianapolis,Corporation
R405	T617	T618	nsubj	patients,exhibit
R4050	T6331	T6326	punct	", ",Corporation
R4051	T6332	T6326	npadvmod	Indiana,Corporation
R4052	T6333	T6326	punct	", ",Corporation
R4053	T6334	T6335	compound	United,States
R4054	T6335	T6326	npadvmod	States,Corporation
R4055	T6336	T6326	punct	),Corporation
R4056	T6337	T6319	punct	", ",glycerol
R4057	T6338	T6339	nummod	1,mM
R4058	T6339	T6319	conj	mM,glycerol
R4059	T6340	T6339	prep	of,mM
R406	T619	T620	amod	clinical,episodes
R4060	T6341	T6340	pobj	phenylmethylsulfonylfluoride,of
R4061	T6342	T6339	punct	", ",mM
R4062	T6343	T6339	cc	and,mM
R4063	T6344	T6345	nummod	1,mM
R4064	T6345	T6339	conj	mM,mM
R4065	T6346	T6345	prep	of,mM
R4066	T6347	T6348	compound	sodium,orthovanadate
R4067	T6348	T6346	pobj	orthovanadate,of
R4068	T6349	T6284	punct	.,homogenized
R4069	T6351	T6352	nsubjpass	Lysates,quantified
R407	T620	T618	dobj	episodes,exhibit
R4070	T6353	T6352	auxpass	were,quantified
R4071	T6354	T6352	prep	by,quantified
R4072	T6355	T6356	compound	Bradford,assay
R4073	T6356	T6354	pobj	assay,by
R4074	T6357	T6356	compound	BCA,assay
R4075	T6358	T6356	compound	protein,assay
R4076	T6359	T6360	punct	(,Rad
R4077	T6360	T6356	parataxis	Rad,assay
R4078	T6361	T6360	compound	Bio,Rad
R4079	T6362	T6360	punct	-,Rad
R408	T621	T622	advmod	often,associated
R4080	T6363	T6360	punct	", ",Rad
R4081	T6364	T6360	npadvmod	Hercules,Rad
R4082	T6365	T6360	punct	", ",Rad
R4083	T6366	T6360	npadvmod	California,Rad
R4084	T6367	T6360	punct	", ",Rad
R4085	T6368	T6369	compound	United,States
R4086	T6369	T6360	npadvmod	States,Rad
R4087	T6370	T6360	punct	),Rad
R4088	T6371	T6352	punct	.,quantified
R4089	T6373	T6374	compound	Protein,lysates
R409	T622	T620	acl	associated,episodes
R4090	T6374	T6375	nsubjpass	lysates,denatured
R4091	T6376	T6375	auxpass	were,denatured
R4092	T6377	T6375	punct	", ",denatured
R4093	T6378	T6375	conj	separated,denatured
R4094	T6379	T6378	prep	in,separated
R4095	T6380	T6381	nummod	8,%
R4096	T6381	T6382	compound	%,PAGE
R4097	T6382	T6379	pobj	PAGE,in
R4098	T6383	T6382	compound	SDS,PAGE
R4099	T6384	T6382	punct	-,PAGE
R41	T121	T122	det	The,mice
R410	T623	T622	prep	with,associated
R4100	T6385	T6378	punct	", ",separated
R4101	T6386	T6378	cc	and,separated
R4102	T6387	T6378	conj	transferred,separated
R4103	T6388	T6387	advmod	overnight,transferred
R4104	T6389	T6387	prep	onto,transferred
R4105	T6390	T6391	det	a,membrane
R4106	T6391	T6389	pobj	membrane,onto
R4107	T6392	T6393	nummod	0.45,μm
R4108	T6393	T6391	compound	μm,membrane
R4109	T6394	T6391	compound	nitrocellulose,membrane
R411	T624	T623	pobj	fasting,with
R4110	T6395	T6396	punct	(,Schleicher
R4111	T6396	T6391	parataxis	Schleicher,membrane
R4112	T6397	T6396	cc	and,Schleicher
R4113	T6398	T6396	conj	Schuell,Schleicher
R4114	T6399	T6396	punct	", ",Schleicher
R4115	T6400	T6396	npadvmod	Keene,Schleicher
R4116	T6401	T6396	punct	", ",Schleicher
R4117	T6402	T6403	compound	New,Hampshire
R4118	T6403	T6396	npadvmod	Hampshire,Schleicher
R4119	T6404	T6396	punct	", ",Schleicher
R412	T625	T618	punct	.,exhibit
R4120	T6405	T6406	compound	United,States
R4121	T6406	T6396	npadvmod	States,Schleicher
R4122	T6407	T6396	punct	),Schleicher
R4123	T6408	T6375	punct	.,denatured
R4124	T6410	T6411	prep	After,immunoblotted
R4125	T6412	T6410	pcomp	blocking,After
R4126	T6413	T6412	prep	with,blocking
R4127	T6414	T6415	nummod	5,%
R4128	T6415	T6416	nmod	%,milk
R4129	T6416	T6413	pobj	milk,with
R413	T627	T628	nsubj	Patients,manifest
R4130	T6417	T6416	amod	nonfat,milk
R4131	T6418	T6412	prep	in,blocking
R4132	T6419	T6420	npadvmod	phosphate,buffered
R4133	T6420	T6422	amod	buffered,saline
R4134	T6421	T6420	punct	-,buffered
R4135	T6422	T6418	pobj	saline,in
R4136	T6423	T6422	prep	with,saline
R4137	T6424	T6425	nummod	0.1,%
R4138	T6425	T6426	compound	%,Tween
R4139	T6426	T6423	pobj	Tween,with
R414	T629	T628	dobj	disease,manifest
R4140	T6427	T6426	punct	-,Tween
R4141	T6428	T6426	nummod	20,Tween
R4142	T6429	T6411	punct	", ",immunoblotted
R4143	T6430	T6431	det	the,membrane
R4144	T6431	T6411	nsubjpass	membrane,immunoblotted
R4145	T6432	T6411	auxpass	was,immunoblotted
R4146	T6433	T6411	advmod	overnight,immunoblotted
R4147	T6434	T6411	prep	at,immunoblotted
R4148	T6435	T6436	nummod	4,°C
R4149	T6436	T6434	pobj	°C,at
R415	T630	T631	advmod	usually,during
R4150	T6437	T6411	prep	with,immunoblotted
R4151	T6438	T6439	quantmod	1,500
R4152	T6439	T6441	nummod	500,dilution
R4153	T6440	T6439	punct	:,500
R4154	T6441	T6437	pobj	dilution,with
R4155	T6442	T6441	prep	of,dilution
R4156	T6443	T6444	det	an,antibody
R4157	T6444	T6442	pobj	antibody,of
R4158	T6445	T6444	amod	anti-MCAD,antibody
R4159	T6446	T6411	punct	.,immunoblotted
R416	T631	T628	prep	during,manifest
R4160	T6448	T6449	nsubjpass	Blots,incubated
R4161	T6450	T6449	auxpass	were,incubated
R4162	T6451	T6449	prep	in,incubated
R4163	T6452	T6453	amod	anti-mouse,antibody
R4164	T6453	T6451	pobj	antibody,in
R4165	T6454	T6453	nmod	IgG,antibody
R4166	T6455	T6456	npadvmod	HRP,conjugated
R4167	T6456	T6453	amod	conjugated,antibody
R4168	T6457	T6456	punct	-,conjugated
R4169	T6458	T6453	amod	secondary,antibody
R417	T632	T633	det	the,years
R4170	T6459	T6449	prep	for,incubated
R4171	T6460	T6461	quantmod	2,4
R4172	T6461	T6463	nummod	4,h
R4173	T6462	T6461	punct	–,4
R4174	T6463	T6459	pobj	h,for
R4175	T6464	T6449	prep	at,incubated
R4176	T6465	T6466	compound	room,temperature
R4177	T6466	T6464	pobj	temperature,at
R4178	T6467	T6449	advcl	using,incubated
R4179	T6468	T6469	amod	standard,procedures
R418	T633	T631	pobj	years,during
R4180	T6469	T6467	dobj	procedures,using
R4181	T6470	T6449	cc	and,incubated
R4182	T6471	T6449	conj	developed,incubated
R4183	T6472	T6471	prep	by,developed
R4184	T6473	T6472	pobj	chemiluminiscence,by
R4185	T6474	T6475	punct	(,Renaissance
R4186	T6475	T6473	parataxis	Renaissance,chemiluminiscence
R4187	T6476	T6475	punct	", ",Renaissance
R4188	T6477	T6478	compound	NEN,Products
R4189	T6478	T6475	appos	Products,Renaissance
R419	T634	T633	amod	first,years
R4190	T6479	T6478	compound	Lifesciences,Products
R4191	T6480	T6475	punct	", ",Renaissance
R4192	T6481	T6475	npadvmod	Boston,Renaissance
R4193	T6482	T6475	punct	", ",Renaissance
R4194	T6483	T6475	npadvmod	Massachusetts,Renaissance
R4195	T6484	T6475	punct	", ",Renaissance
R4196	T6485	T6486	compound	United,States
R4197	T6486	T6475	npadvmod	States,Renaissance
R4198	T6487	T6475	punct	),Renaissance
R4199	T6488	T6449	punct	.,incubated
R42	T153	T149	acl	seen,lesions
R420	T635	T633	nummod	two,years
R4200	T6565	T6566	compound	Liver,enzyme
R4201	T6566	T6567	compound	enzyme,activity
R4202	T6568	T6567	punct	.,activity
R4203	T6570	T6571	prep	In,prepared
R4204	T6572	T6570	pobj	order,In
R4205	T6573	T6574	aux	to,evaluate
R4206	T6574	T6572	acl	evaluate,order
R4207	T6575	T6576	compound	MCAD,activity
R4208	T6576	T6574	dobj	activity,evaluate
R4209	T6577	T6574	prep	in,evaluate
R421	T636	T633	prep	of,years
R4210	T6578	T6577	pobj	mice,in
R4211	T6579	T6571	punct	", ",prepared
R4212	T6580	T6581	compound	liver,homogenates
R4213	T6581	T6571	nsubjpass	homogenates,prepared
R4214	T6582	T6571	auxpass	were,prepared
R4215	T6583	T6571	prep	from,prepared
R4216	T6584	T6585	nmod	MCAD,mice
R4217	T6585	T6583	pobj	mice,from
R4218	T6586	T6584	punct	+,MCAD
R4219	T6587	T6584	punct	/,MCAD
R422	T637	T636	pobj	life,of
R4220	T6588	T6584	punct	+,MCAD
R4221	T6589	T6590	punct	(,5
R4222	T6590	T6584	parataxis	5,MCAD
R4223	T6591	T6590	nsubj	n,5
R4224	T6592	T6590	punct	=,5
R4225	T6593	T6590	punct	),5
R4226	T6594	T6584	cc	and,MCAD
R4227	T6595	T6584	conj	MCAD,MCAD
R4228	T6596	T6595	punct	−,MCAD
R4229	T6597	T6595	punct	/,MCAD
R423	T638	T628	punct	.,manifest
R4230	T6598	T6595	punct	−,MCAD
R4231	T6599	T6600	punct	(,5
R4232	T6600	T6585	parataxis	5,mice
R4233	T6601	T6600	nsubj	n,5
R4234	T6602	T6600	punct	=,5
R4235	T6603	T6600	punct	),5
R4236	T6604	T6571	punct	.,prepared
R4237	T6606	T6607	det	The,assay
R4238	T6607	T6615	nsubjpass	assay,used
R4239	T6608	T6607	amod	sensitive,assay
R424	T640	T641	nsubj	Symptoms,include
R4240	T6609	T6608	cc	and,sensitive
R4241	T6610	T6611	advmod	highly,specific
R4242	T6611	T6608	conj	specific,sensitive
R4243	T6612	T6613	amod	anaerobic,ETF
R4244	T6613	T6607	compound	ETF,assay
R4245	T6614	T6607	compound	reduction,assay
R4246	T6616	T6615	auxpass	was,used
R4247	T6617	T6615	prep	on,used
R4248	T6618	T6619	compound	tissue,extracts
R4249	T6619	T6617	pobj	extracts,on
R425	T642	T643	amod	hypoketotic,hypoglycemia
R4250	T6620	T6619	prep	with,extracts
R4251	T6621	T6622	compound	octanoyl,CoA
R4252	T6622	T6620	pobj	CoA,with
R4253	T6623	T6622	punct	-,CoA
R4254	T6624	T6625	punct	(,C8
R4255	T6625	T6622	parataxis	C8,CoA
R4256	T6626	T6625	punct	),C8
R4257	T6627	T6622	cc	and,CoA
R4258	T6628	T6629	compound	palmitoyl,CoA
R4259	T6629	T6622	conj	CoA,CoA
R426	T643	T641	dobj	hypoglycemia,include
R4260	T6630	T6629	punct	-,CoA
R4261	T6631	T6632	punct	(,C16
R4262	T6632	T6629	parataxis	C16,CoA
R4263	T6633	T6632	punct	),C16
R4264	T6634	T6622	prep	as,CoA
R4265	T6635	T6634	pobj	substrate,as
R4266	T6636	T6637	mark	as,described
R4267	T6637	T6615	advcl	described,used
R4268	T6638	T6637	advmod	previously,described
R4269	T6639	T6640	punct	[,29
R427	T644	T643	cc	and,hypoglycemia
R4270	T6640	T6615	parataxis	29,used
R4271	T6641	T6640	punct	],29
R4272	T6642	T6615	punct	.,used
R4273	T6683	T6682	cc	and,Fasting
R4274	T6684	T6685	compound	cold,challenge
R4275	T6685	T6682	conj	challenge,Fasting
R4276	T6686	T6685	punct	.,challenge
R4277	T6688	T6689	nummod	Eight,wk
R4278	T6689	T6691	npadvmod	wk,old
R4279	T6690	T6689	punct	-,wk
R428	T645	T646	npadvmod	Reye,like
R4280	T6691	T6693	amod	old,mice
R4281	T6692	T6691	punct	-,old
R4282	T6693	T6703	nsubjpass	mice,fasted
R4283	T6694	T6693	nmod	MCAD,mice
R4284	T6695	T6694	punct	+,MCAD
R4285	T6696	T6694	punct	/,MCAD
R4286	T6697	T6694	punct	+,MCAD
R4287	T6698	T6694	cc	and,MCAD
R4288	T6699	T6694	conj	MCAD,MCAD
R4289	T6700	T6699	punct	−,MCAD
R429	T646	T648	amod	like,episodes
R4290	T6701	T6699	punct	/,MCAD
R4291	T6702	T6699	punct	−,MCAD
R4292	T6704	T6703	auxpass	were,fasted
R4293	T6705	T6703	prep	for,fasted
R4294	T6706	T6707	nummod	18,h
R4295	T6707	T6705	pobj	h,for
R4296	T6708	T6709	punct	(,experiments
R4297	T6709	T6707	parataxis	experiments,h
R4298	T6710	T6711	compound	cold,tolerance
R4299	T6711	T6709	compound	tolerance,experiments
R43	T154	T153	prep	in,seen
R430	T647	T646	punct	-,like
R4300	T6712	T6709	punct	),experiments
R4301	T6713	T6707	cc	or,h
R4302	T6714	T6715	nummod	24,h
R4303	T6715	T6707	conj	h,h
R4304	T6716	T6717	punct	(,experiments
R4305	T6717	T6715	parataxis	experiments,h
R4306	T6718	T6719	nmod	serum,glucose
R4307	T6719	T6717	nmod	glucose,experiments
R4308	T6720	T6719	punct	", ",glucose
R4309	T6721	T6722	amod	free,acid
R431	T648	T643	conj	episodes,hypoglycemia
R4310	T6722	T6719	conj	acid,glucose
R4311	T6723	T6722	amod	fatty,acid
R4312	T6724	T6722	punct	", ",acid
R4313	T6725	T6726	amod	organic,acid
R4314	T6726	T6722	conj	acid,acid
R4315	T6727	T6726	punct	", ",acid
R4316	T6728	T6726	cc	and,acid
R4317	T6729	T6726	conj	carnitine,acid
R4318	T6730	T6717	punct	),experiments
R4319	T6731	T6732	amod	prior,to
R432	T649	T650	punct	[,1
R4320	T6732	T6703	prep	to,fasted
R4321	T6733	T6732	pobj	analysis,to
R4322	T6734	T6703	punct	.,fasted
R4323	T6736	T6737	compound	Glucose,concentration
R4324	T6737	T6738	nsubjpass	concentration,measured
R4325	T6739	T6738	auxpass	was,measured
R4326	T6740	T6738	prep	in,measured
R4327	T6741	T6742	nummod	10,μl
R4328	T6742	T6743	compound	μl,sera
R4329	T6743	T6740	pobj	sera,in
R433	T650	T641	parataxis	1,include
R4330	T6744	T6738	advcl	using,measured
R4331	T6745	T6746	det	an,system
R4332	T6746	T6744	dobj	system,using
R4333	T6747	T6748	nmod	Ektachem,DT
R4334	T6748	T6746	nmod	DT,system
R4335	T6749	T6748	nummod	II,DT
R4336	T6750	T6751	punct	(,Diagnostics
R4337	T6751	T6746	parataxis	Diagnostics,system
R4338	T6752	T6751	nmod	Johnson,Diagnostics
R4339	T6753	T6752	cc	and,Johnson
R434	T651	T650	punct	],1
R4340	T6754	T6752	conj	Johnson,Johnson
R4341	T6755	T6751	compound	Clinical,Diagnostics
R4342	T6756	T6751	punct	", ",Diagnostics
R4343	T6757	T6751	npadvmod	Rochester,Diagnostics
R4344	T6758	T6751	punct	", ",Diagnostics
R4345	T6759	T6760	compound	New,York
R4346	T6760	T6751	npadvmod	York,Diagnostics
R4347	T6761	T6751	punct	", ",Diagnostics
R4348	T6762	T6763	compound	United,States
R4349	T6763	T6751	npadvmod	States,Diagnostics
R435	T652	T641	punct	.,include
R4350	T6764	T6751	punct	),Diagnostics
R4351	T6765	T6738	punct	.,measured
R4352	T6767	T6768	amod	Total,acids
R4353	T6768	T6771	nsubjpass	acids,measured
R4354	T6769	T6768	amod	non-esterified,acids
R4355	T6770	T6768	amod	fatty,acids
R4356	T6772	T6768	punct	(,acids
R4357	T6773	T6768	appos	NEFA,acids
R4358	T6774	T6771	punct	),measured
R4359	T6775	T6771	auxpass	were,measured
R436	T654	T655	nsubjpass	It,estimated
R4360	T6776	T6771	prep	by,measured
R4361	T6777	T6778	det	an,method
R4362	T6778	T6776	pobj	method,by
R4363	T6779	T6778	amod	enzymatic,method
R4364	T6780	T6778	punct	", ",method
R4365	T6781	T6778	amod	colorimetric,method
R4366	T6782	T6783	punct	(,Diagnostics
R4367	T6783	T6771	parataxis	Diagnostics,measured
R4368	T6784	T6783	punct	“,Diagnostics
R4369	T6785	T6786	nmod	NEFA,C
R437	T656	T655	auxpass	is,estimated
R4370	T6786	T6788	nmod	C,reagents
R4371	T6787	T6786	punct	-,C
R4372	T6788	T6783	dep	reagents,Diagnostics
R4373	T6789	T6788	punct	”,reagents
R4374	T6790	T6783	punct	", ",Diagnostics
R4375	T6791	T6783	compound	Wako,Diagnostics
R4376	T6792	T6783	punct	", ",Diagnostics
R4377	T6793	T6783	npadvmod	Richmond,Diagnostics
R4378	T6794	T6783	punct	", ",Diagnostics
R4379	T6795	T6783	npadvmod	Virginia,Diagnostics
R438	T657	T658	mark	that,die
R4380	T6796	T6783	punct	", ",Diagnostics
R4381	T6797	T6798	compound	United,States
R4382	T6798	T6783	npadvmod	States,Diagnostics
R4383	T6799	T6783	punct	),Diagnostics
R4384	T6800	T6771	punct	.,measured
R4385	T6802	T6803	det	The,assay
R4386	T6803	T6804	nsubjpass	assay,modified
R4387	T6805	T6804	auxpass	was,modified
R4388	T6806	T6807	aux	to,accommodate
R4389	T6807	T6804	advcl	accommodate,modified
R439	T658	T655	ccomp	die,estimated
R4390	T6808	T6809	det	a,size
R4391	T6809	T6807	dobj	size,accommodate
R4392	T6810	T6809	amod	reduced,size
R4393	T6811	T6809	compound	sample,size
R4394	T6812	T6813	punct	(,μl
R4395	T6813	T6809	parataxis	μl,size
R4396	T6814	T6813	nummod	10,μl
R4397	T6815	T6813	punct	),μl
R4398	T6816	T6809	cc	and,size
R4399	T6817	T6809	conj	use,size
R44	T155	T156	nmod	MCAD,mice
R440	T659	T660	advmod	approximately,59
R4400	T6818	T6817	prep	of,use
R4401	T6819	T6820	det	a,reader
R4402	T6820	T6818	pobj	reader,of
R4403	T6821	T6820	compound	microplate,reader
R4404	T6822	T6820	prep	for,reader
R4405	T6823	T6822	pobj	measurement,for
R4406	T6824	T6823	prep	of,measurement
R4407	T6825	T6826	amod	optical,density
R4408	T6826	T6824	pobj	density,of
R4409	T6827	T6826	prep	at,density
R441	T660	T661	nummod	59,%
R4410	T6828	T6829	nummod	550,nm
R4411	T6829	T6827	pobj	nm,at
R4412	T6830	T6804	punct	.,modified
R4413	T6832	T6833	nmod	Urine,acid
R4414	T6833	T6835	compound	acid,analyses
R4415	T6834	T6833	amod	organic,acid
R4416	T6835	T6836	nsubjpass	analyses,performed
R4417	T6837	T6836	auxpass	were,performed
R4418	T6838	T6836	advcl	using,performed
R4419	T6839	T6840	compound	gas,chromatography
R442	T661	T658	nsubj	%,die
R4420	T6840	T6838	dobj	chromatography,using
R4421	T6841	T6840	punct	-,chromatography
R4422	T6842	T6843	compound	mass,spectroscopy
R4423	T6843	T6840	appos	spectroscopy,chromatography
R4424	T6844	T6845	mark	as,described
R4425	T6845	T6838	advcl	described,using
R4426	T6846	T6845	advmod	previously,described
R4427	T6847	T6848	punct	[,30
R4428	T6848	T6836	parataxis	30,performed
R4429	T6849	T6848	nummod	22,30
R443	T662	T661	prep	of,%
R4430	T6850	T6848	punct	",",30
R4431	T6851	T6848	punct	],30
R4432	T6852	T6836	punct	", ",performed
R4433	T6853	T6836	prep	except,performed
R4434	T6854	T6855	nsubjpass	tetracosane,used
R4435	T6855	T6853	pcomp	used,except
R4436	T6856	T6857	punct	(,Sigma
R4437	T6857	T6854	parataxis	Sigma,tetracosane
R4438	T6858	T6857	dep	C24,Sigma
R4439	T6859	T6857	punct	;,Sigma
R444	T663	T662	pobj	patients,of
R4440	T6860	T6857	punct	", ",Sigma
R4441	T6861	T6862	compound	St.,Louis
R4442	T6862	T6857	npadvmod	Louis,Sigma
R4443	T6863	T6857	punct	", ",Sigma
R4444	T6864	T6857	npadvmod	Missouri,Sigma
R4445	T6865	T6857	punct	", ",Sigma
R4446	T6866	T6867	compound	United,States
R4447	T6867	T6857	npadvmod	States,Sigma
R4448	T6868	T6857	punct	),Sigma
R4449	T6869	T6855	auxpass	was,used
R445	T664	T663	acl	presenting,patients
R4450	T6870	T6855	prep	as,used
R4451	T6871	T6872	det	the,standard
R4452	T6872	T6870	pobj	standard,as
R4453	T6873	T6872	amod	internal,standard
R4454	T6874	T6836	punct	", ",performed
R4455	T6875	T6836	cc	and,performed
R4456	T6876	T6877	amod	quantitative,determinations
R4457	T6877	T6878	nsubjpass	determinations,made
R4458	T6878	T6836	conj	made,performed
R4459	T6879	T6878	auxpass	were,made
R446	T665	T664	advmod	clinically,presenting
R4460	T6880	T6878	prep	based,made
R4461	T6881	T6880	prep	on,based
R4462	T6882	T6881	pobj	comparison,on
R4463	T6883	T6882	prep	with,comparison
R4464	T6884	T6885	amod	synthetic,standards
R4465	T6885	T6883	pobj	standards,with
R4466	T6886	T6885	compound	calibration,standards
R4467	T6887	T6878	punct	.,made
R4468	T6889	T6890	compound	Acylcarnitine,analyses
R4469	T6890	T6891	nsubjpass	analyses,conducted
R447	T666	T664	prep	between,presenting
R4470	T6892	T6890	prep	in,analyses
R4471	T6893	T6892	pobj	serum,in
R4472	T6894	T6893	cc	and,serum
R4473	T6895	T6893	conj	bile,serum
R4474	T6896	T6891	auxpass	were,conducted
R4475	T6897	T6891	advcl	using,conducted
R4476	T6898	T6899	nmod	electrospray,spectrometry
R4477	T6899	T6897	dobj	spectrometry,using
R4478	T6900	T6899	amod	tandem,spectrometry
R4479	T6901	T6899	compound	mass,spectrometry
R448	T667	T668	quantmod	15,26
R4480	T6902	T6903	punct	[,32
R4481	T6903	T6891	parataxis	32,conducted
R4482	T6904	T6903	nummod	31,32
R4483	T6905	T6903	punct	",",32
R4484	T6906	T6903	punct	],32
R4485	T6907	T6891	punct	.,conducted
R4486	T6958	T6957	punct	.,Histopathology
R4487	T6960	T6961	nummod	Twelve,mice
R4488	T6961	T6962	nsubjpass	mice,examined
R4489	T6963	T6962	auxpass	were,examined
R449	T668	T670	nummod	26,mo
R4490	T6964	T6962	prep	for,examined
R4491	T6965	T6966	amod	gross,abnormalities
R4492	T6966	T6964	pobj	abnormalities,for
R4493	T6967	T6965	cc	and,gross
R4494	T6968	T6965	conj	histologic,gross
R4495	T6969	T6962	punct	", ",examined
R4496	T6970	T6962	prep	including,examined
R4497	T6971	T6970	pobj	one,including
R4498	T6972	T6971	amod	male,one
R4499	T6973	T6971	cc	and,one
R45	T156	T154	pobj	mice,in
R450	T669	T668	quantmod	to,26
R4500	T6974	T6975	nummod	one,mouse
R4501	T6975	T6971	conj	mouse,one
R4502	T6976	T6974	amod	female,one
R4503	T6977	T6975	nmod	MCAD,mouse
R4504	T6978	T6977	punct	−,MCAD
R4505	T6979	T6977	punct	/,MCAD
R4506	T6980	T6977	punct	−,MCAD
R4507	T6981	T6982	nummod	18,mo
R4508	T6982	T6984	npadvmod	mo,old
R4509	T6983	T6982	punct	-,mo
R451	T670	T666	pobj	mo,between
R4510	T6984	T6971	amod	old,one
R4511	T6985	T6984	punct	-,old
R4512	T6986	T6971	punct	", ",one
R4513	T6987	T6971	conj	one,one
R4514	T6988	T6987	amod	male,one
R4515	T6989	T6987	cc	and,one
R4516	T6990	T6991	nummod	one,mouse
R4517	T6991	T6987	conj	mouse,one
R4518	T6992	T6990	amod	female,one
R4519	T6993	T6991	nmod	MCAD,mouse
R452	T671	T670	prep	of,mo
R4520	T6994	T6993	punct	+,MCAD
R4521	T6995	T6993	punct	/,MCAD
R4522	T6996	T6993	punct	+,MCAD
R4523	T6997	T6998	nummod	6,mo
R4524	T6998	T7000	npadvmod	mo,old
R4525	T6999	T6998	punct	-,mo
R4526	T7000	T6987	amod	old,one
R4527	T7001	T7000	punct	-,old
R4528	T7002	T6987	punct	", ",one
R4529	T7003	T6987	conj	two,one
R453	T672	T671	pobj	age,of
R4530	T7004	T7003	amod	male,two
R4531	T7005	T7003	cc	and,two
R4532	T7006	T7007	nummod	two,mice
R4533	T7007	T7003	conj	mice,two
R4534	T7008	T7006	amod	female,two
R4535	T7009	T7007	nmod	MCAD,mice
R4536	T7010	T7009	punct	−,MCAD
R4537	T7011	T7009	punct	/,MCAD
R4538	T7012	T7009	punct	−,MCAD
R4539	T7013	T7014	nummod	4,wk
R454	T673	T658	prep	during,die
R4540	T7014	T7016	npadvmod	wk,old
R4541	T7015	T7014	punct	-,wk
R4542	T7016	T7003	amod	old,two
R4543	T7017	T7016	punct	-,old
R4544	T7018	T7003	punct	", ",two
R4545	T7019	T7003	cc	and,two
R4546	T7020	T7003	conj	two,two
R4547	T7021	T7020	amod	male,two
R4548	T7022	T7020	cc	and,two
R4549	T7023	T7024	nummod	two,mice
R455	T674	T675	poss	their,episode
R4550	T7024	T7020	conj	mice,two
R4551	T7025	T7023	amod	female,two
R4552	T7026	T7024	nmod	MCAD,mice
R4553	T7027	T7026	punct	+,MCAD
R4554	T7028	T7026	punct	/,MCAD
R4555	T7029	T7026	punct	+,MCAD
R4556	T7030	T7031	nummod	4,wk
R4557	T7031	T7033	npadvmod	wk,old
R4558	T7032	T7031	punct	-,wk
R4559	T7033	T7020	amod	old,two
R456	T675	T673	pobj	episode,during
R4560	T7034	T7033	punct	-,old
R4561	T7035	T6962	punct	.,examined
R4562	T7037	T7038	nsubjpass	Kidney,fixed
R4563	T7039	T7037	punct	", ",Kidney
R4564	T7040	T7037	conj	spleen,Kidney
R4565	T7041	T7040	punct	", ",spleen
R4566	T7042	T7040	conj	pancreas,spleen
R4567	T7043	T7042	punct	", ",pancreas
R4568	T7044	T7042	conj	liver,pancreas
R4569	T7045	T7044	punct	", ",liver
R457	T676	T675	amod	first,episode
R4570	T7046	T7044	conj	brain,liver
R4571	T7047	T7046	punct	", ",brain
R4572	T7048	T7046	conj	heart,brain
R4573	T7049	T7048	punct	", ",heart
R4574	T7050	T7048	conj	testicles,heart
R4575	T7051	T7050	punct	", ",testicles
R4576	T7052	T7050	conj	ovaries,testicles
R4577	T7053	T7052	punct	", ",ovaries
R4578	T7054	T7052	cc	and,ovaries
R4579	T7055	T7056	amod	skeletal,muscle
R458	T677	T675	amod	clinical,episode
R4580	T7056	T7052	conj	muscle,ovaries
R4581	T7057	T7038	auxpass	were,fixed
R4582	T7058	T7038	prep	by,fixed
R4583	T7059	T7058	pobj	immersion,by
R4584	T7060	T7059	prep	in,immersion
R4585	T7061	T7062	amod	buffered,formalin
R4586	T7062	T7060	pobj	formalin,in
R4587	T7063	T7064	nummod	10,%
R4588	T7064	T7062	compound	%,formalin
R4589	T7065	T7038	punct	", ",fixed
R459	T678	T679	punct	[,1
R4590	T7066	T7038	conj	processed,fixed
R4591	T7067	T7066	advmod	routinely,processed
R4592	T7068	T7066	prep	for,processed
R4593	T7069	T7070	compound	paraffin,sectioning
R4594	T7070	T7068	pobj	sectioning,for
R4595	T7071	T7066	punct	", ",processed
R4596	T7072	T7066	conj	sectioned,processed
R4597	T7073	T7072	prep	at,sectioned
R4598	T7074	T7075	nummod	5,μm
R4599	T7075	T7073	pobj	μm,at
R46	T157	T155	punct	−,MCAD
R460	T679	T655	parataxis	1,estimated
R4600	T7076	T7072	punct	", ",sectioned
R4601	T7077	T7072	cc	and,sectioned
R4602	T7078	T7072	conj	stained,sectioned
R4603	T7079	T7078	prep	with,stained
R4604	T7080	T7079	pobj	hematoxylin,with
R4605	T7081	T7080	cc	and,hematoxylin
R4606	T7082	T7080	conj	eosin,hematoxylin
R4607	T7083	T7038	punct	.,fixed
R4608	T7085	T7086	amod	Frozen,sections
R4609	T7086	T7088	nsubjpass	sections,prepared
R461	T680	T679	punct	],1
R4610	T7087	T7086	compound	liver,sections
R4611	T7089	T7088	auxpass	were,prepared
R4612	T7090	T7088	advcl	using,prepared
R4613	T7091	T7092	amod	standard,methods
R4614	T7092	T7090	dobj	methods,using
R4615	T7093	T7088	cc	and,prepared
R4616	T7094	T7095	nsubjpass	sections,stained
R4617	T7095	T7088	conj	stained,prepared
R4618	T7096	T7095	auxpass	were,stained
R4619	T7097	T7095	prep	with,stained
R462	T681	T655	punct	.,estimated
R4620	T7098	T7099	nmod	Oil,O
R4621	T7099	T7097	pobj	O,with
R4622	T7100	T7099	punct	-,O
R4623	T7101	T7099	amod	red,O
R4624	T7102	T7099	punct	-,O
R4625	T7103	T7095	punct	.,stained
R4626	T7105	T7106	nsubjpass	Slides,examined
R4627	T7107	T7106	auxpass	were,examined
R4628	T7108	T7106	prep	without,examined
R4629	T7109	T7108	pobj	knowledge,without
R463	T683	T684	det	The,pathogenesis
R4630	T7110	T7109	prep	of,knowledge
R4631	T7111	T7110	pobj	genotype,of
R4632	T7112	T7111	cc	or,genotype
R4633	T7113	T7111	conj	age,genotype
R4634	T7114	T7106	punct	.,examined
R4635	T7116	T7117	amod	Other,mice
R4636	T7117	T7118	nsubjpass	mice,examined
R4637	T7119	T7118	auxpass	were,examined
R4638	T7120	T7118	prep	because,examined
R4639	T7121	T7120	pcomp	of,because
R464	T684	T685	nsubjpass	pathogenesis,understood
R4640	T7122	T7123	amod	sporadic,disease
R4641	T7123	T7120	pobj	disease,because
R4642	T7124	T7123	amod	clinical,disease
R4643	T7125	T7118	punct	.,examined
R4644	T7127	T7128	nsubjpass	Those,evaluated
R4645	T7129	T7127	prep	with,Those
R4646	T7130	T7131	amod	visible,enlargement
R4647	T7131	T7129	pobj	enlargement,with
R4648	T7132	T7131	amod	cardiac,enlargement
R4649	T7133	T7128	auxpass	were,evaluated
R465	T686	T684	prep	of,pathogenesis
R4650	T7134	T7128	prep	for,evaluated
R4651	T7135	T7136	amod	cardiac,lesions
R4652	T7136	T7134	pobj	lesions,for
R4653	T7137	T7128	punct	.,evaluated
R4654	T7201	T7202	amod	Statistical,analyses
R4655	T7203	T7202	punct	.,analyses
R4656	T7205	T7206	nsubjpass	Results,tested
R4657	T7207	T7205	prep	between,Results
R4658	T7208	T7207	pobj	groups,between
R4659	T7209	T7206	auxpass	were,tested
R466	T687	T688	det	the,range
R4660	T7210	T7206	prep	for,tested
R4661	T7211	T7212	amod	statistical,significance
R4662	T7212	T7210	pobj	significance,for
R4663	T7213	T7206	advcl	using,tested
R4664	T7214	T7215	poss	Student,test
R4665	T7215	T7213	dobj	test,using
R4666	T7216	T7214	case	's,Student
R4667	T7217	T7215	compound	t,test
R4668	T7218	T7215	punct	-,test
R4669	T7219	T7206	punct	.,tested
R467	T688	T686	pobj	range,of
R4670	T7221	T7222	det	A,p
R4671	T7222	T7223	nsubjpass	p,set
R4672	T7224	T7225	punct	<,0.05
R4673	T7225	T7222	amod	0.05,p
R4674	T7226	T7223	auxpass	was,set
R4675	T7227	T7223	prep	as,set
R4676	T7228	T7227	amod	significant,as
R4677	T7229	T7223	punct	.,set
R4678	T7248	T7247	prep	for,Strategy
R4679	T7249	T7248	pobj	Disruption,for
R468	T689	T688	amod	wide,range
R4680	T7250	T7249	prep	of,Disruption
R4681	T7251	T7252	det	the,Gene
R4682	T7252	T7250	pobj	Gene,of
R4683	T7253	T7252	compound	Mouse,Gene
R4684	T7254	T7252	compound	Acadm,Gene
R4685	T7256	T7257	punct	(,A
R4686	T7257	T7258	meta	A,vector
R4687	T7259	T7257	punct	),A
R4688	T7260	T7258	det	The,vector
R4689	T7261	T7262	compound	MCAD,insertion
R469	T690	T688	prep	of,range
R4690	T7262	T7258	compound	insertion,vector
R4691	T7263	T7262	compound	IV2,insertion
R4692	T7264	T7258	compound	targeting,vector
R4693	T7265	T7258	prep	with,vector
R4694	T7266	T7267	det	a,region
R4695	T7267	T7265	pobj	region,with
R4696	T7268	T7267	amod	deleted,region
R4697	T7269	T7270	nummod	1.3,kb
R4698	T7270	T7267	compound	kb,region
R4699	T7271	T7270	punct	-,kb
R47	T158	T155	punct	/,MCAD
R470	T691	T692	amod	metabolic,disturbances
R4700	T7272	T7267	acl	encompassing,region
R4701	T7273	T7272	dobj	exon,encompassing
R4702	T7274	T7273	nummod	10,exon
R4703	T7275	T7273	cc	and,exon
R4704	T7276	T7277	amod	flanking,sequences
R4705	T7277	T7273	conj	sequences,exon
R4706	T7278	T7258	punct	.,vector
R4707	T7280	T7281	compound	MCAD,IV2
R4708	T7281	T7282	nsubj	IV2,undergoes
R4709	T7283	T7284	compound	gap,repair
R471	T692	T690	pobj	disturbances,of
R4710	T7284	T7282	dobj	repair,undergoes
R4711	T7285	T7282	prep	upon,undergoes
R4712	T7286	T7287	amod	homologous,recombination
R4713	T7287	T7285	pobj	recombination,upon
R4714	T7288	T7282	prep	at,undergoes
R4715	T7289	T7290	det	the,locus
R4716	T7290	T7288	pobj	locus,at
R4717	T7291	T7290	amod	endogenous,locus
R4718	T7292	T7290	compound	Acadm,locus
R4719	T7293	T7282	advcl	resulting,undergoes
R472	T693	T692	prep	in,disturbances
R4720	T7294	T7293	prep	in,resulting
R4721	T7295	T7296	det	a,duplication
R4722	T7296	T7294	pobj	duplication,in
R4723	T7297	T7296	prep	of,duplication
R4724	T7298	T7299	nmod	exons,8
R4725	T7299	T7297	pobj	8,of
R4726	T7300	T7299	punct	", ",8
R4727	T7301	T7299	conj	9,8
R4728	T7302	T7301	punct	", ",9
R4729	T7303	T7301	cc	and,9
R473	T694	T695	compound	MCAD,deficiency
R4730	T7304	T7301	conj	10,9
R4731	T7305	T7296	prep	at,duplication
R4732	T7306	T7307	det	the,allele
R4733	T7307	T7305	pobj	allele,at
R4734	T7308	T7307	amod	disrupted,allele
R4735	T7309	T7282	punct	.,undergoes
R4736	T7311	T7312	punct	(,B
R4737	T7312	T7313	meta	B,analysis
R4738	T7314	T7312	punct	),B
R4739	T7315	T7316	compound	Southern,blot
R474	T695	T693	pobj	deficiency,in
R4740	T7316	T7313	compound	blot,analysis
R4741	T7317	T7313	prep	of,analysis
R4742	T7318	T7319	npadvmod	EcoRI,digested
R4743	T7319	T7321	amod	digested,DNA
R4744	T7320	T7319	punct	-,digested
R4745	T7321	T7317	pobj	DNA,of
R4746	T7322	T7321	amod	genomic,DNA
R4747	T7323	T7321	prep	from,DNA
R4748	T7324	T7325	compound	ES,cells
R4749	T7325	T7323	pobj	cells,from
R475	T696	T685	auxpass	is,understood
R4750	T7326	T7325	acl	screened,cells
R4751	T7327	T7326	prep	by,screened
R4752	T7328	T7327	pobj	PCR,by
R4753	T7329	T7313	punct	.,analysis
R4754	T7331	T7332	compound	Probe,A
R4755	T7332	T7333	nsubj	A,hybridizes
R4756	T7334	T7332	punct	", ",A
R4757	T7335	T7336	det	a,fragment
R4758	T7336	T7332	appos	fragment,A
R4759	T7337	T7336	compound	DNA,fragment
R476	T697	T685	advmod	poorly,understood
R4760	T7338	T7336	acl	consisting,fragment
R4761	T7339	T7338	prep	of,consisting
R4762	T7340	T7341	det	a,portion
R4763	T7341	T7339	pobj	portion,of
R4764	T7342	T7341	prep	of,portion
R4765	T7343	T7342	pobj	exon,of
R4766	T7344	T7343	nummod	10,exon
R4767	T7345	T7346	dep	that,is
R4768	T7346	T7341	relcl	is,portion
R4769	T7347	T7346	neg	not,is
R477	T698	T685	cc	and,understood
R4770	T7348	T7346	acomp	present,is
R4771	T7349	T7346	prep	in,is
R4772	T7350	T7351	det	the,vector
R4773	T7351	T7349	pobj	vector,in
R4774	T7352	T7351	compound	targeting,vector
R4775	T7353	T7333	punct	", ",hybridizes
R4776	T7354	T7333	prep	to,hybridizes
R4777	T7355	T7356	det	an,fragment
R4778	T7356	T7354	pobj	fragment,to
R4779	T7357	T7356	amod	endogenous,fragment
R478	T699	T700	amod	certain,aspects
R4780	T7358	T7359	nummod	3.1,kb
R4781	T7359	T7356	compound	kb,fragment
R4782	T7360	T7359	punct	-,kb
R4783	T7361	T7333	cc	and,hybridizes
R4784	T7362	T7333	punct	", ",hybridizes
R4785	T7363	T7364	prep	upon,to
R4786	T7364	T7333	conj	to,hybridizes
R4787	T7365	T7366	amod	homologous,recombination
R4788	T7366	T7363	pobj	recombination,upon
R4789	T7367	T7364	punct	", ",to
R479	T700	T701	nsubj	aspects,are
R4790	T7368	T7369	det	a,fragment
R4791	T7369	T7364	pobj	fragment,to
R4792	T7370	T7371	nummod	13.2,kb
R4793	T7371	T7369	compound	kb,fragment
R4794	T7372	T7371	punct	-,kb
R4795	T7373	T7333	punct	.,hybridizes
R4796	T7375	T7376	nsubj	Lane,represents
R4797	T7377	T7375	nummod	1,Lane
R4798	T7378	T7379	det	a,line
R4799	T7379	T7376	dobj	line,represents
R48	T159	T155	punct	−,MCAD
R480	T701	T685	conj	are,understood
R4800	T7380	T7381	amod	wild,type
R4801	T7381	T7379	compound	type,line
R4802	T7382	T7381	punct	-,type
R4803	T7383	T7379	compound	ES,line
R4804	T7384	T7379	compound	cell,line
R4805	T7385	T7376	punct	", ",represents
R4806	T7386	T7376	cc	and,represents
R4807	T7387	T7388	nmod	Lane,2
R4808	T7388	T7389	nsubj	2,represent
R4809	T7389	T7376	conj	represent,represents
R481	T702	T700	prep	of,aspects
R4810	T7390	T7388	cc	and,2
R4811	T7391	T7388	conj	3,2
R4812	T7392	T7393	amod	targeted,lines
R4813	T7393	T7389	dobj	lines,represent
R4814	T7394	T7393	compound	ES,lines
R4815	T7395	T7393	compound	cell,lines
R4816	T7396	T7389	punct	.,represent
R4817	T7455	T7456	compound	Northern,Blot
R4818	T7456	T7457	compound	Blot,Analysis
R4819	T7458	T7457	prep	from,Analysis
R482	T703	T704	compound	patient,management
R4820	T7459	T7460	nmod	MCAD,Mice
R4821	T7460	T7458	pobj	Mice,from
R4822	T7461	T7459	punct	−,MCAD
R4823	T7462	T7459	punct	/,MCAD
R4824	T7463	T7459	punct	−,MCAD
R4825	T7464	T7465	punct	(,2
R4826	T7465	T7459	parataxis	2,MCAD
R4827	T7466	T7465	nsubj	n,2
R4828	T7467	T7465	punct	=,2
R4829	T7468	T7465	punct	),2
R483	T704	T702	pobj	management,of
R4830	T7469	T7459	cc	and,MCAD
R4831	T7470	T7459	conj	MCAD,MCAD
R4832	T7471	T7470	punct	+,MCAD
R4833	T7472	T7470	punct	/,MCAD
R4834	T7473	T7470	punct	+,MCAD
R4835	T7474	T7475	punct	(,2
R4836	T7475	T7470	parataxis	2,MCAD
R4837	T7476	T7475	nsubj	n,2
R4838	T7477	T7475	punct	=,2
R4839	T7478	T7475	punct	),2
R484	T705	T701	acomp	controversial,are
R4840	T7480	T7481	compound	Acadm,message
R4841	T7481	T7482	nsubjpass	message,detected
R4842	T7483	T7482	auxpass	was,detected
R4843	T7484	T7482	prep	from,detected
R4844	T7485	T7486	det	the,heart
R4845	T7486	T7484	pobj	heart,from
R4846	T7487	T7486	punct	", ",heart
R4847	T7488	T7486	conj	liver,heart
R4848	T7489	T7488	punct	", ",liver
R4849	T7490	T7491	amod	brown,fat
R485	T706	T701	punct	.,are
R4850	T7491	T7488	conj	fat,liver
R4851	T7492	T7491	punct	", ",fat
R4852	T7493	T7491	conj	brain,fat
R4853	T7494	T7493	punct	", ",brain
R4854	T7495	T7493	conj	kidney,brain
R4855	T7496	T7495	punct	", ",kidney
R4856	T7497	T7495	cc	and,kidney
R4857	T7498	T7495	conj	muscle,kidney
R4858	T7499	T7486	punct	(,heart
R4859	T7500	T7486	cc	and,heart
R486	T708	T709	det	An,model
R4860	T7501	T7502	amod	white,fat
R4861	T7502	T7486	conj	fat,heart
R4862	T7503	T7502	cc	and,fat
R4863	T7504	T7502	conj	testes,fat
R4864	T7505	T7506	punct	", ",shown
R4865	T7506	T7486	parataxis	shown,heart
R4866	T7507	T7506	nsubj	data,shown
R4867	T7508	T7506	neg	not,shown
R4868	T7509	T7506	punct	),shown
R4869	T7510	T7486	prep	of,heart
R487	T709	T711	nsubj	model,is
R4870	T7511	T7512	amod	only,mice
R4871	T7512	T7510	pobj	mice,of
R4872	T7513	T7512	nmod	MCAD,mice
R4873	T7514	T7513	punct	+,MCAD
R4874	T7515	T7513	punct	/,MCAD
R4875	T7516	T7513	punct	+,MCAD
R4876	T7517	T7482	punct	.,detected
R4877	T7519	T7520	advmod	Most,expression
R4878	T7520	T7522	nsubj	expression,occurred
R4879	T7521	T7520	amod	robust,expression
R488	T710	T709	compound	animal,model
R4880	T7523	T7522	prep	in,occurred
R4881	T7524	T7525	amod	brown,fat
R4882	T7525	T7523	pobj	fat,in
R4883	T7526	T7525	punct	", ",fat
R4884	T7527	T7525	conj	kidney,fat
R4885	T7528	T7527	punct	", ",kidney
R4886	T7529	T7527	conj	heart,kidney
R4887	T7530	T7529	punct	", ",heart
R4888	T7531	T7529	cc	and,heart
R4889	T7532	T7533	amod	skeletal,muscle
R489	T712	T709	prep	for,model
R4890	T7533	T7529	conj	muscle,heart
R4891	T7534	T7522	punct	.,occurred
R4892	T7536	T7537	nmod	MCAD,mice
R4893	T7537	T7541	nsubj	mice,had
R4894	T7538	T7536	punct	−,MCAD
R4895	T7539	T7536	punct	/,MCAD
R4896	T7540	T7536	punct	−,MCAD
R4897	T7542	T7543	det	no,message
R4898	T7543	T7541	dobj	message,had
R4899	T7544	T7543	amod	detectable,message
R49	T160	T152	aux	have,reported
R490	T713	T714	compound	MCAD,deficiency
R4900	T7545	T7541	prep	in,had
R4901	T7546	T7547	det	all,tissues
R4902	T7547	T7545	pobj	tissues,in
R4903	T7548	T7547	acl	examined,tissues
R4904	T7549	T7541	punct	.,had
R4905	T7639	T7638	prep	of,Immunoblots
R4906	T7640	T7641	compound	Liver,Homogenates
R4907	T7641	T7639	pobj	Homogenates,of
R4908	T7642	T7638	prep	from,Immunoblots
R4909	T7643	T7644	nmod	MCAD,Mice
R491	T714	T712	pobj	deficiency,for
R4910	T7644	T7642	pobj	Mice,from
R4911	T7645	T7643	punct	+,MCAD
R4912	T7646	T7643	punct	/,MCAD
R4913	T7647	T7643	punct	+,MCAD
R4914	T7648	T7643	cc	and,MCAD
R4915	T7649	T7643	conj	MCAD,MCAD
R4916	T7650	T7649	punct	−,MCAD
R4917	T7651	T7649	punct	/,MCAD
R4918	T7652	T7649	punct	−,MCAD
R4919	T7654	T7655	nsubjpass	These,probed
R492	T715	T711	acomp	essential,is
R4920	T7656	T7655	auxpass	were,probed
R4921	T7657	T7655	prep	with,probed
R4922	T7658	T7659	amod	anti-MCAD,antibody
R4923	T7659	T7657	pobj	antibody,with
R4924	T7660	T7659	cc	or,antibody
R4925	T7661	T7662	amod	anti-SCAD,antibody
R4926	T7662	T7659	conj	antibody,antibody
R4927	T7663	T7655	punct	.,probed
R4928	T7665	T7666	amod	Homozygous,mice
R4929	T7666	T7671	nsubj	mice,had
R493	T716	T717	aux	to,understand
R4930	T7667	T7666	nmod	MCAD,mice
R4931	T7668	T7667	punct	−,MCAD
R4932	T7669	T7667	punct	/,MCAD
R4933	T7670	T7667	punct	−,MCAD
R4934	T7672	T7673	det	no,protein
R4935	T7673	T7671	dobj	protein,had
R4936	T7674	T7673	amod	detectable,protein
R4937	T7675	T7673	compound	MCAD,protein
R4938	T7676	T7671	punct	.,had
R4939	T7678	T7679	compound	MCAD,protein
R494	T717	T711	advcl	understand,is
R4940	T7679	T7680	nsubj	protein,is
R4941	T7681	T7682	advmod	only,detectable
R4942	T7682	T7680	acomp	detectable,is
R4943	T7683	T7680	prep	under,is
R4944	T7684	T7685	det	the,lane
R4945	T7685	T7683	pobj	lane,under
R4946	T7686	T7685	nmod	MCAD,lane
R4947	T7687	T7688	npadvmod	protein,spiked
R4948	T7688	T7685	amod	spiked,lane
R4949	T7689	T7688	punct	–,spiked
R495	T718	T717	advmod	better,understand
R4950	T7690	T7691	punct	(,control
R4951	T7691	T7685	parataxis	control,lane
R4952	T7692	T7691	amod	positive,control
R4953	T7693	T7691	punct	),control
R4954	T7694	T7680	punct	.,is
R4955	T7696	T7697	prep	As,revealed
R4956	T7698	T7699	det	a,analysis
R4957	T7699	T7696	pobj	analysis,As
R4958	T7700	T7699	compound	control,analysis
R4959	T7701	T7697	punct	", ",revealed
R496	T719	T720	det	the,pathogenesis
R4960	T7702	T7703	compound	liver,homogenates
R4961	T7703	T7697	nsubj	homogenates,revealed
R4962	T7704	T7703	acl	probed,homogenates
R4963	T7705	T7704	prep	with,probed
R4964	T7706	T7707	amod	anti-SCAD,antibody
R4965	T7707	T7705	pobj	antibody,with
R4966	T7708	T7709	mark	that,was
R4967	T7709	T7697	ccomp	was,revealed
R4968	T7710	T7711	compound	SCAD,protein
R4969	T7711	T7709	nsubj	protein,was
R497	T720	T717	dobj	pathogenesis,understand
R4970	T7712	T7709	acomp	present,was
R4971	T7713	T7709	prep	in,was
R4972	T7714	T7715	preconj	both,MCAD
R4973	T7715	T7716	nmod	MCAD,mice
R4974	T7716	T7713	pobj	mice,in
R4975	T7717	T7715	punct	+,MCAD
R4976	T7718	T7715	punct	/,MCAD
R4977	T7719	T7715	punct	+,MCAD
R4978	T7720	T7715	cc	and,MCAD
R4979	T7721	T7715	conj	MCAD,MCAD
R498	T721	T720	prep	of,pathogenesis
R4980	T7722	T7721	punct	−,MCAD
R4981	T7723	T7721	punct	/,MCAD
R4982	T7724	T7721	punct	−,MCAD
R4983	T7725	T7697	punct	.,revealed
R4984	T7727	T7728	det	No,protein
R4985	T7728	T7733	nsubjpass	protein,detected
R4986	T7729	T7728	nmod	MCAD,protein
R4987	T7730	T7731	amod	positive,control
R4988	T7731	T7728	compound	control,protein
R4989	T7732	T7731	punct	-,control
R499	T722	T723	compound	MCAD,deficiency
R4990	T7734	T7733	auxpass	is,detected
R4991	T7735	T7733	prep	by,detected
R4992	T7736	T7737	amod	anti-SCAD,antibodies
R4993	T7737	T7735	pobj	antibodies,by
R4994	T7738	T7739	punct	(,lane
R4995	T7739	T7733	parataxis	lane,detected
R4996	T7740	T7739	compound	MCAD,lane
R4997	T7741	T7739	punct	),lane
R4998	T7742	T7733	punct	.,detected
R4999	T7745	T7744	punct	", ",mw
R5	T82	T69	prep	in,is
R50	T161	T152	neg	not,reported
R500	T723	T721	pobj	deficiency,of
R5000	T7746	T7747	amod	molecular,standards
R5001	T7747	T7744	appos	standards,mw
R5002	T7748	T7747	compound	weight,standards
R5003	T7749	T7744	punct	.,mw
R5004	T7814	T7815	compound	Acylcarnitine,Analyses
R5005	T7817	T7818	punct	(,A
R5006	T7818	T7819	meta	A,analysis
R5007	T7820	T7818	punct	),A
R5008	T7821	T7822	compound	Serum,acylcarnitine
R5009	T7822	T7819	compound	acylcarnitine,analysis
R501	T724	T717	cc	and,understand
R5010	T7823	T7819	prep	of,analysis
R5011	T7824	T7825	nmod	MCAD,mice
R5012	T7825	T7823	pobj	mice,of
R5013	T7826	T7824	punct	+,MCAD
R5014	T7827	T7824	punct	/,MCAD
R5015	T7828	T7824	punct	+,MCAD
R5016	T7829	T7830	punct	(,4
R5017	T7830	T7824	parataxis	4,MCAD
R5018	T7831	T7830	nsubj	n,4
R5019	T7832	T7830	punct	=,4
R502	T725	T726	aux	to,develop
R5020	T7833	T7830	punct	),4
R5021	T7834	T7824	cc	and,MCAD
R5022	T7835	T7824	conj	MCAD,MCAD
R5023	T7836	T7835	punct	−,MCAD
R5024	T7837	T7835	punct	/,MCAD
R5025	T7838	T7835	punct	−,MCAD
R5026	T7839	T7840	punct	(,4
R5027	T7840	T7825	parataxis	4,mice
R5028	T7841	T7840	nsubj	n,4
R5029	T7842	T7840	punct	=,4
R503	T726	T717	conj	develop,understand
R5030	T7843	T7840	punct	),4
R5031	T7845	T7846	expl	There,are
R5032	T7847	T7848	amod	significant,elevations
R5033	T7848	T7846	attr	elevations,are
R5034	T7849	T7846	prep	in,are
R5035	T7850	T7851	compound	acylcarnitine,species
R5036	T7851	T7849	pobj	species,in
R5037	T7852	T7853	mark	as,indicated
R5038	T7853	T7846	advcl	indicated,are
R5039	T7854	T7853	prep	in,indicated
R504	T727	T728	amod	better,regimens
R5040	T7855	T7856	nmod	MCAD,mice
R5041	T7856	T7854	pobj	mice,in
R5042	T7857	T7855	punct	−,MCAD
R5043	T7858	T7855	punct	/,MCAD
R5044	T7859	T7855	punct	−,MCAD
R5045	T7860	T7846	punct	.,are
R5046	T7862	T7863	nsubj	Values,are
R5047	T7864	T7862	acl	shown,Values
R5048	T7865	T7866	compound	mean,values
R5049	T7866	T7863	attr	values,are
R505	T728	T726	dobj	regimens,develop
R5050	T7867	T7866	punct	±,values
R5051	T7868	T7869	amod	standard,deviation
R5052	T7869	T7866	appos	deviation,values
R5053	T7870	T7869	punct	(,deviation
R5054	T7871	T7869	appos	SD,deviation
R5055	T7872	T7863	punct	),are
R5056	T7873	T7863	punct	.,are
R5057	T7875	T7876	nsubj	Asterisk,indicates
R5058	T7877	T7876	dobj	p,indicates
R5059	T7878	T7879	punct	<,0.002
R506	T729	T728	compound	management,regimens
R5060	T7879	T7877	amod	0.002,p
R5061	T7880	T7876	cc	and,indicates
R5062	T7881	T7882	nsubj	‡,indicates
R5063	T7882	T7876	conj	indicates,indicates
R5064	T7883	T7882	dobj	p,indicates
R5065	T7884	T7885	punct	<,0.01
R5066	T7885	T7883	amod	0.01,p
R5067	T7886	T7882	punct	.,indicates
R5068	T7888	T7889	punct	(,B
R5069	T7889	T7890	meta	B,are
R507	T730	T728	prep	for,regimens
R5070	T7891	T7889	punct	),B
R5071	T7892	T7890	expl	There,are
R5072	T7893	T7894	amod	significant,elevations
R5073	T7894	T7890	attr	elevations,are
R5074	T7895	T7894	prep	in,elevations
R5075	T7896	T7897	compound	bile,acylcarnitines
R5076	T7897	T7895	pobj	acylcarnitines,in
R5077	T7898	T7897	prep	of,acylcarnitines
R5078	T7899	T7900	det	the,mice
R5079	T7900	T7898	pobj	mice,of
R508	T731	T732	amod	human,patients
R5080	T7901	T7900	amod	same,mice
R5081	T7902	T7900	acl	shown,mice
R5082	T7903	T7902	prep	in,shown
R5083	T7904	T7903	punct	(,in
R5084	T7905	T7903	pobj	A,in
R5085	T7906	T7902	punct	),shown
R5086	T7907	T7908	mark	as,indicated
R5087	T7908	T7902	advcl	indicated,shown
R5088	T7909	T7890	punct	.,are
R5089	T7911	T7912	nsubj	Values,are
R509	T732	T730	pobj	patients,for
R5090	T7913	T7911	acl	shown,Values
R5091	T7914	T7915	compound	mean,values
R5092	T7915	T7912	attr	values,are
R5093	T7916	T7915	punct	±,values
R5094	T7917	T7915	appos	SD,values
R5095	T7918	T7912	punct	.,are
R5096	T7920	T7921	nsubj	Asterisk,indicates
R5097	T7922	T7921	dobj	p,indicates
R5098	T7923	T7924	punct	<,0.001
R5099	T7924	T7922	amod	0.001,p
R51	T162	T152	auxpass	been,reported
R510	T733	T711	punct	.,is
R5100	T7925	T7921	punct	.,indicates
R5101	T7927	T7928	punct	(,C
R5102	T7928	T7929	meta	C,profile
R5103	T7930	T7928	punct	),C
R5104	T7931	T7932	compound	Bile,acylcarnitine
R5105	T7932	T7929	compound	acylcarnitine,profile
R5106	T7933	T7929	prep	of,profile
R5107	T7934	T7935	det	an,mouse
R5108	T7935	T7933	pobj	mouse,of
R5109	T7936	T7935	nmod	MCAD,mouse
R511	T735	T736	aux	To,gain
R5110	T7937	T7936	punct	−,MCAD
R5111	T7938	T7936	punct	/,MCAD
R5112	T7939	T7936	punct	−,MCAD
R5113	T7940	T7929	prep	compared,profile
R5114	T7941	T7940	prep	to,compared
R5115	T7942	T7943	det	a,patient
R5116	T7943	T7941	pobj	patient,to
R5117	T7944	T7943	amod	human,patient
R5118	T7945	T7943	prep	with,patient
R5119	T7946	T7947	compound	MCAD,deficiency
R512	T736	T737	advcl	gain,developed
R5120	T7947	T7945	pobj	deficiency,with
R5121	T7948	T7929	punct	.,profile
R5122	T7950	T7951	amod	Internal,standards
R5123	T7951	T7952	nsubjpass	standards,indicated
R5124	T7953	T7952	auxpass	are,indicated
R5125	T7954	T7952	prep	by,indicated
R5126	T7955	T7956	det	an,asterisk
R5127	T7956	T7954	pobj	asterisk,by
R5128	T7957	T7952	punct	.,indicated
R5129	T8010	T8009	prep	of,Histopathology
R513	T738	T739	amod	further,insight
R5130	T8011	T8012	nmod	MCAD,Mice
R5131	T8012	T8010	pobj	Mice,of
R5132	T8013	T8011	punct	+,MCAD
R5133	T8014	T8011	punct	/,MCAD
R5134	T8015	T8011	punct	+,MCAD
R5135	T8016	T8011	cc	and,MCAD
R5136	T8017	T8011	conj	MCAD,MCAD
R5137	T8018	T8017	punct	−,MCAD
R5138	T8019	T8017	punct	/,MCAD
R5139	T8020	T8017	punct	−,MCAD
R514	T739	T736	dobj	insight,gain
R5140	T8022	T8023	punct	(,A
R5141	T8023	T8024	meta	A,had
R5142	T8025	T8023	punct	),A
R5143	T8026	T8027	nmod	MCAD,mice
R5144	T8027	T8024	nsubj	mice,had
R5145	T8028	T8026	punct	+,MCAD
R5146	T8029	T8026	punct	/,MCAD
R5147	T8030	T8026	punct	+,MCAD
R5148	T8031	T8032	det	no,evidence
R5149	T8032	T8024	dobj	evidence,had
R515	T740	T739	prep	into,insight
R5150	T8033	T8032	prep	of,evidence
R5151	T8034	T8035	amod	hepatic,steatosis
R5152	T8035	T8033	pobj	steatosis,of
R5153	T8036	T8024	prep	following,had
R5154	T8037	T8038	det	a,fast
R5155	T8038	T8036	pobj	fast,following
R5156	T8039	T8040	nummod	24,h
R5157	T8040	T8038	compound	h,fast
R5158	T8041	T8040	punct	-,h
R5159	T8042	T8024	punct	.,had
R516	T741	T742	det	the,mechanisms
R5160	T8044	T8045	compound	Liver,section
R5161	T8046	T8045	prep	with,section
R5162	T8047	T8048	nmod	Oil,O
R5163	T8048	T8051	compound	O,stain
R5164	T8049	T8048	punct	-,O
R5165	T8050	T8048	amod	Red,O
R5166	T8051	T8046	pobj	stain,with
R5167	T8052	T8045	punct	.,section
R5168	T8054	T8055	punct	(,B
R5169	T8055	T8056	meta	B,Hepatosteatosis
R517	T742	T740	pobj	mechanisms,into
R5170	T8057	T8055	punct	),B
R5171	T8058	T8056	prep	in,Hepatosteatosis
R5172	T8059	T8060	compound	MCAD,−
R5173	T8060	T8063	compound	−,mouse
R5174	T8061	T8060	compound	−,−
R5175	T8062	T8060	punct	/,−
R5176	T8063	T8058	pobj	mouse,in
R5177	T8064	T8056	prep	following,Hepatosteatosis
R5178	T8065	T8066	det	a,fast
R5179	T8066	T8064	pobj	fast,following
R518	T743	T742	prep	of,mechanisms
R5180	T8067	T8068	nummod	24,h
R5181	T8068	T8066	compound	h,fast
R5182	T8069	T8068	punct	-,h
R5183	T8070	T8056	punct	.,Hepatosteatosis
R5184	T8072	T8073	nmod	Oil,O
R5185	T8073	T8076	npadvmod	O,stained
R5186	T8074	T8073	punct	-,O
R5187	T8075	T8073	amod	Red,O
R5188	T8076	T8077	amod	stained,sections
R5189	T8077	T8079	nsubj	sections,revealed
R519	T744	T745	det	this,disease
R5190	T8078	T8077	compound	liver,sections
R5191	T8080	T8081	amod	severe,steatosis
R5192	T8081	T8079	dobj	steatosis,revealed
R5193	T8082	T8080	cc	and,severe
R5194	T8083	T8080	conj	diffuse,severe
R5195	T8084	T8081	amod	microvesicular,steatosis
R5196	T8085	T8084	cc	and,microvesicular
R5197	T8086	T8084	conj	macrovesicular,microvesicular
R5198	T8087	T8081	amod	hepatic,steatosis
R5199	T8088	T8079	prep	in,revealed
R52	T163	T152	prep	in,reported
R520	T745	T743	pobj	disease,of
R5200	T8089	T8090	nmod	MCAD,mice
R5201	T8090	T8088	pobj	mice,in
R5202	T8091	T8089	punct	−,MCAD
R5203	T8092	T8089	punct	/,MCAD
R5204	T8093	T8089	punct	−,MCAD
R5205	T8094	T8079	punct	.,revealed
R5206	T8096	T8097	punct	(,C
R5207	T8097	T8098	meta	C,cardiomyopathy
R5208	T8099	T8097	cc	and,C
R5209	T8100	T8097	conj	D,C
R521	T746	T737	punct	", ",developed
R5210	T8101	T8100	punct	),D
R5211	T8102	T8098	amod	Diffuse,cardiomyopathy
R5212	T8103	T8098	prep	with,cardiomyopathy
R5213	T8104	T8105	amod	chronic,degeneration
R5214	T8105	T8103	pobj	degeneration,with
R5215	T8106	T8105	amod	active,degeneration
R5216	T8107	T8105	amod	multifocal,degeneration
R5217	T8108	T8105	compound	myocyte,degeneration
R5218	T8109	T8105	cc	and,degeneration
R5219	T8110	T8105	conj	necrosis,degeneration
R522	T747	T737	nsubj	we,developed
R5220	T8111	T8098	prep	in,cardiomyopathy
R5221	T8112	T8113	nmod	MCAD,mice
R5222	T8113	T8111	pobj	mice,in
R5223	T8114	T8112	punct	−,MCAD
R5224	T8115	T8112	punct	/,MCAD
R5225	T8116	T8112	punct	−,MCAD
R5226	T8117	T8098	punct	.,cardiomyopathy
R5227	T8162	T8161	prep	of,Characteristics
R5228	T8163	T8164	npadvmod	MCAD,Deficient
R5229	T8164	T8166	amod	Deficient,Mice
R523	T748	T749	det	a,model
R5230	T8165	T8164	punct	-,Deficient
R5231	T8166	T8162	pobj	Mice,of
R524	T749	T737	dobj	model,developed
R525	T750	T749	compound	mouse,model
R526	T751	T749	prep	of,model
R527	T752	T753	compound	MCAD,deficiency
R528	T753	T751	pobj	deficiency,of
R529	T754	T737	prep	by,developed
R53	T164	T165	amod	human,patients
R530	T755	T756	compound	gene,targeting
R531	T756	T754	pobj	targeting,by
R532	T757	T756	prep	in,targeting
R533	T758	T759	amod	embryonic,stem
R534	T759	T760	nmod	stem,cells
R535	T760	T757	pobj	cells,in
R536	T761	T759	punct	(,stem
R537	T762	T759	appos	ES,stem
R538	T763	T760	punct	),cells
R539	T764	T765	punct	(,14
R54	T165	T163	pobj	patients,in
R540	T765	T737	parataxis	14,developed
R541	T766	T765	prep	for,14
R542	T767	T766	pobj	reviews,for
R543	T768	T765	punct	[,14
R544	T769	T765	nummod	13,14
R545	T770	T765	punct	",",14
R546	T771	T765	punct	],14
R547	T772	T765	punct	),14
R548	T773	T737	punct	.,developed
R549	T775	T776	det	The,mice
R55	T166	T165	compound	MCAD,patients
R550	T776	T778	nsubj	mice,had
R551	T777	T776	compound	mutant,mice
R552	T779	T780	amod	many,features
R553	T780	T778	dobj	features,had
R554	T781	T780	amod	relevant,features
R555	T782	T780	amod	characteristic,features
R556	T783	T782	prep	of,characteristic
R557	T784	T785	det	the,disease
R558	T785	T783	pobj	disease,of
R559	T786	T780	acl	found,features
R56	T167	T152	punct	.,reported
R560	T787	T786	prep	in,found
R561	T788	T789	amod	human,patients
R562	T789	T787	pobj	patients,in
R563	T790	T791	npadvmod	MCAD,deficient
R564	T791	T789	amod	deficient,patients
R565	T792	T791	punct	-,deficient
R566	T793	T780	punct	", ",features
R567	T794	T780	cc	along,features
R568	T795	T794	dep	with,along
R569	T796	T797	det	some,findings
R57	T169	T170	expl	There,was
R570	T797	T780	conj	findings,features
R571	T798	T797	amod	unexpected,findings
R572	T799	T778	punct	.,had
R579	T964	T965	compound	Gene,Targeting
R58	T171	T172	amod	significant,mortality
R580	T966	T965	cc	and,Targeting
R581	T967	T965	conj	Generation,Targeting
R582	T968	T967	prep	of,Generation
R583	T969	T970	npadvmod	MCAD,Deficient
R584	T970	T972	amod	Deficient,Mice
R585	T971	T970	punct	-,Deficient
R586	T972	T968	pobj	Mice,of
R587	T974	T975	compound	MCAD,vector
R588	T975	T977	nsubjpass	vector,designed
R589	T976	T975	compound	insertion,vector
R59	T172	T170	attr	mortality,was
R590	T978	T975	punct	(,vector
R591	T979	T980	compound	MCAD,IV2
R592	T980	T975	appos	IV2,vector
R593	T981	T977	punct	),designed
R594	T982	T977	auxpass	was,designed
R595	T983	T984	aux	to,undergo
R596	T984	T977	advcl	undergo,designed
R597	T985	T986	compound	gap,repair
R598	T986	T984	dobj	repair,undergo
R599	T987	T986	prep	of,repair
R6	T83	T82	pobj	humans,in
R60	T173	T172	amod	neonatal,mortality
R600	T988	T989	det	the,region
R601	T989	T987	pobj	region,of
R602	T990	T991	nummod	1.3,kb
R603	T991	T989	nmod	kb,region
R604	T992	T991	punct	-,kb
R605	T993	T989	amod	deleted,region
R606	T994	T984	prep	upon,undergo
R607	T995	T996	amod	homologous,recombination
R608	T996	T994	pobj	recombination,upon
R609	T997	T984	prep	in,undergo
R61	T174	T172	prep	of,mortality
R610	T998	T999	nmod	129P2,cells
R611	T999	T997	pobj	cells,in
R612	T1000	T1001	punct	(,OlaHsd
R613	T1001	T999	parataxis	OlaHsd,cells
R614	T1002	T1001	compound	129P2,OlaHsd
R615	T1003	T1001	punct	/,OlaHsd
R616	T1004	T1001	punct	),OlaHsd
R617	T1005	T999	compound	ES,cells
R618	T1006	T999	appos	E14,cells
R619	T1007	T1006	punct	–,E14
R62	T175	T176	nmod	MCAD,pups
R620	T1008	T1006	nummod	1,E14
R621	T1009	T977	punct	.,designed
R622	T1011	T1012	amod	Correct,targeting
R623	T1012	T1013	nsubj	targeting,resulted
R624	T1014	T1012	prep	of,targeting
R625	T1015	T1016	det	the,locus
R626	T1016	T1014	pobj	locus,of
R627	T1017	T1016	compound	MCAD,locus
R628	T1018	T1013	prep	in,resulted
R629	T1019	T1020	det	a,duplication
R63	T176	T174	pobj	pups,of
R630	T1020	T1018	pobj	duplication,in
R631	T1021	T1020	prep	of,duplication
R632	T1022	T1023	nmod	exons,8
R633	T1023	T1021	pobj	8,of
R634	T1024	T1023	punct	", ",8
R635	T1025	T1023	conj	9,8
R636	T1026	T1025	punct	", ",9
R637	T1027	T1025	cc	and,9
R638	T1028	T1025	conj	10,9
R639	T1029	T1020	cc	and,duplication
R64	T177	T175	punct	−,MCAD
R640	T1030	T1020	conj	integration,duplication
R641	T1031	T1030	prep	of,integration
R642	T1032	T1033	amod	flanking,plasmid
R643	T1033	T1031	pobj	plasmid,of
R644	T1034	T1033	cc	and,plasmid
R645	T1035	T1036	compound	Neo,sequences
R646	T1036	T1033	conj	sequences,plasmid
R647	T1037	T1038	punct	(,Figure
R648	T1038	T1013	parataxis	Figure,resulted
R649	T1039	T1038	nummod	1A,Figure
R65	T178	T175	punct	/,MCAD
R650	T1040	T1038	punct	),Figure
R651	T1041	T1013	punct	.,resulted
R652	T1043	T1044	det	The,vector
R653	T1044	T1046	nsubjpass	vector,designed
R654	T1045	T1044	compound	insertion,vector
R655	T1047	T1046	auxpass	was,designed
R656	T1048	T1049	aux	to,duplicate
R657	T1049	T1046	advcl	duplicate,designed
R658	T1050	T1051	nmod	exon,8
R659	T1051	T1049	dobj	8,duplicate
R66	T179	T175	punct	−,MCAD
R660	T1052	T1051	punct	", ",8
R661	T1053	T1051	conj	9,8
R662	T1054	T1053	punct	", ",9
R663	T1055	T1053	cc	and,9
R664	T1056	T1053	conj	10,9
R665	T1057	T1049	prep	at,duplicate
R666	T1058	T1059	det	the,locus
R667	T1059	T1057	pobj	locus,at
R668	T1060	T1059	compound	MCAD,locus
R669	T1061	T1046	punct	.,designed
R67	T180	T172	acl	demonstrating,mortality
R670	T1063	T1064	nsubj	Translation,results
R671	T1065	T1063	prep	of,Translation
R672	T1066	T1067	det	the,region
R673	T1067	T1065	pobj	region,of
R674	T1068	T1067	amod	duplicated,region
R675	T1069	T1067	nmod	exon,region
R676	T1070	T1069	nummod	8,exon
R677	T1071	T1064	prep	in,results
R678	T1072	T1073	det	the,formation
R679	T1073	T1071	pobj	formation,in
R68	T181	T180	dobj	similarities,demonstrating
R680	T1074	T1073	prep	of,formation
R681	T1075	T1076	amod	premature,codons
R682	T1076	T1074	pobj	codons,of
R683	T1077	T1076	compound	stop,codons
R684	T1078	T1073	acl	resulting,formation
R685	T1079	T1078	prep	in,resulting
R686	T1080	T1079	pobj	truncation,in
R687	T1081	T1080	prep	of,truncation
R688	T1082	T1083	det	the,monomer
R689	T1083	T1081	pobj	monomer,of
R69	T182	T181	prep	to,similarities
R690	T1084	T1083	compound	MCAD,monomer
R691	T1085	T1064	punct	.,results
R692	T1087	T1088	advmod	Specifically,arises
R693	T1089	T1088	punct	", ",arises
R694	T1090	T1091	det	the,codon
R695	T1091	T1088	nsubj	codon,arises
R696	T1092	T1091	amod	first,codon
R697	T1093	T1091	amod	premature,codon
R698	T1094	T1091	compound	stop,codon
R699	T1095	T1088	prep	after,arises
R7	T84	T69	punct	.,is
R70	T183	T182	pobj	patterns,to
R700	T1096	T1095	pobj	translation,after
R701	T1097	T1096	prep	of,translation
R702	T1098	T1099	advmod	only,seven
R703	T1099	T1100	nummod	seven,acids
R704	T1100	T1097	pobj	acids,of
R705	T1101	T1100	compound	amino,acids
R706	T1102	T1100	prep	from,acids
R707	T1103	T1104	det	the,exon
R708	T1104	T1102	pobj	exon,from
R709	T1105	T1104	amod	duplicated,exon
R71	T184	T183	prep	of,patterns
R710	T1106	T1104	nummod	8,exon
R711	T1107	T1088	punct	.,arises
R712	T1109	T1110	det	The,monomer
R713	T1110	T1113	nsubj	monomer,missing
R714	T1111	T1110	amod	resulting,monomer
R715	T1112	T1110	compound	MCAD,monomer
R716	T1114	T1113	aux	is,missing
R717	T1115	T1116	det	the,helixes
R718	T1116	T1113	dobj	helixes,missing
R719	T1117	T1118	npadvmod	C,terminal
R72	T185	T186	amod	clinical,episodes
R720	T1118	T1120	amod	terminal,domain
R721	T1119	T1118	punct	-,terminal
R722	T1120	T1116	compound	domain,helixes
R723	T1121	T1116	compound	α,helixes
R724	T1122	T1116	punct	-,helixes
R725	T1123	T1124	dep	that,are
R726	T1124	T1116	relcl	are,helixes
R727	T1125	T1124	acomp	responsible,are
R728	T1126	T1125	prep	for,responsible
R729	T1127	T1126	pcomp	making,for
R73	T186	T184	pobj	episodes,of
R730	T1128	T1129	compound	intersubunit,contacts
R731	T1129	T1127	dobj	contacts,making
R732	T1130	T1131	aux	to,generate
R733	T1131	T1127	advcl	generate,making
R734	T1132	T1133	det	the,homotetramer
R735	T1133	T1131	dobj	homotetramer,generate
R736	T1134	T1133	amod	functional,homotetramer
R737	T1135	T1133	compound	MCAD,homotetramer
R738	T1136	T1113	punct	.,missing
R739	T1138	T1139	compound	ES,cell
R74	T187	T186	cc	and,episodes
R740	T1139	T1140	compound	cell,clones
R741	T1140	T1141	nsubjpass	clones,screened
R742	T1142	T1141	auxpass	were,screened
R743	T1143	T1141	prep	by,screened
R744	T1144	T1143	pobj	PCR,by
R745	T1145	T1146	punct	(,shown
R746	T1146	T1141	parataxis	shown,screened
R747	T1147	T1146	nsubj	data,shown
R748	T1148	T1146	neg	not,shown
R749	T1149	T1146	punct	),shown
R75	T188	T186	conj	mortality,episodes
R750	T1150	T1141	cc	and,screened
R751	T1151	T1141	conj	confirmed,screened
R752	T1152	T1151	prep	by,confirmed
R753	T1153	T1154	compound	Southern,blot
R754	T1154	T1155	compound	blot,analysis
R755	T1155	T1152	pobj	analysis,by
R756	T1156	T1141	punct	.,screened
R757	T1158	T1159	compound	Southern,blot
R758	T1159	T1160	compound	blot,analysis
R759	T1160	T1161	nsubj	analysis,used
R76	T189	T183	prep	in,patterns
R760	T1162	T1163	det	an,probe
R761	T1163	T1161	dobj	probe,used
R762	T1164	T1163	nmod	exon,probe
R763	T1165	T1164	nummod	10,exon
R764	T1166	T1167	punct	(,A
R765	T1167	T1163	parataxis	A,probe
R766	T1168	T1167	compound	probe,A
R767	T1169	T1167	punct	),A
R768	T1170	T1163	punct	", ",probe
R769	T1171	T1172	neg	not,present
R77	T190	T191	npadvmod	MCAD,deficient
R770	T1172	T1163	relcl	present,probe
R771	T1173	T1172	prep	in,present
R772	T1174	T1175	det	the,vector
R773	T1175	T1173	pobj	vector,in
R774	T1176	T1175	compound	targeting,vector
R775	T1177	T1163	punct	", ",probe
R776	T1178	T1163	acl	hybridized,probe
R777	T1179	T1178	prep	to,hybridized
R778	T1180	T1181	det	a,band
R779	T1181	T1179	pobj	band,to
R78	T191	T193	amod	deficient,patients
R780	T1182	T1183	nummod	13.2,kb
R781	T1183	T1181	compound	kb,band
R782	T1184	T1183	punct	-,kb
R783	T1185	T1178	prep	in,hybridized
R784	T1186	T1185	pobj	addition,in
R785	T1187	T1186	prep	to,addition
R786	T1188	T1189	det	the,band
R787	T1189	T1187	pobj	band,to
R788	T1190	T1191	nummod	3.1,kb
R789	T1191	T1189	nmod	kb,band
R79	T192	T191	punct	-,deficient
R790	T1192	T1191	punct	-,kb
R791	T1193	T1189	amod	endogenous,band
R792	T1194	T1189	acl	indicating,band
R793	T1195	T1196	amod	targeted,insertion
R794	T1196	T1194	dobj	insertion,indicating
R795	T1197	T1196	prep	of,insertion
R796	T1198	T1199	det	the,vector
R797	T1199	T1197	pobj	vector,of
R798	T1200	T1196	prep	at,insertion
R799	T1201	T1202	det	the,locus
R8	T86	T87	aux	To,understand
R80	T193	T189	pobj	patients,in
R800	T1202	T1200	pobj	locus,at
R801	T1203	T1202	compound	Acadm,locus
R802	T1204	T1205	punct	(,Figure
R803	T1205	T1161	parataxis	Figure,used
R804	T1206	T1205	nummod	1B,Figure
R805	T1207	T1205	punct	),Figure
R806	T1208	T1161	punct	.,used
R807	T1210	T1211	advmod	Correctly,targeted
R808	T1211	T1212	amod	targeted,clones
R809	T1212	T1215	nsubjpass	clones,microinjected
R81	T194	T170	punct	.,was
R810	T1213	T1214	compound	ES,cell
R811	T1214	T1212	compound	cell,clones
R812	T1216	T1215	auxpass	were,microinjected
R813	T1217	T1215	prep	into,microinjected
R814	T1218	T1219	nmod	B6,blastocysts
R815	T1219	T1217	pobj	blastocysts,into
R816	T1220	T1219	punct	(,blastocysts
R817	T1221	T1222	nmod	C57BL,6NTac
R818	T1222	T1219	nmod	6NTac,blastocysts
R819	T1223	T1222	punct	/,6NTac
R82	T196	T197	det	The,mouse
R820	T1224	T1219	punct	),blastocysts
R821	T1225	T1226	aux	to,generate
R822	T1226	T1215	advcl	generate,microinjected
R823	T1227	T1228	amod	chimeric,mice
R824	T1228	T1226	dobj	mice,generate
R825	T1229	T1215	punct	.,microinjected
R826	T1231	T1232	amod	Chimeric,mice
R827	T1232	T1233	nsubjpass	mice,backcrossed
R828	T1234	T1233	auxpass	were,backcrossed
R829	T1235	T1233	prep	to,backcrossed
R83	T197	T201	nsubj	mouse,reproduced
R830	T1236	T1237	preconj	both,129P2
R831	T1237	T1238	npadvmod	129P2,inbred
R832	T1238	T1241	amod	inbred,mice
R833	T1239	T1237	cc	and,129P2
R834	T1240	T1237	conj	B6,129P2
R835	T1241	T1235	pobj	mice,to
R836	T1242	T1243	aux	to,produce
R837	T1243	T1233	advcl	produce,backcrossed
R838	T1244	T1245	nmod	MCAD,mice
R839	T1245	T1243	dobj	mice,produce
R84	T198	T199	npadvmod	MCAD,deficient
R840	T1246	T1244	punct	+,MCAD
R841	T1247	T1244	punct	/,MCAD
R842	T1248	T1244	punct	−,MCAD
R843	T1249	T1244	cc	and,MCAD
R844	T1250	T1251	advmod	eventually,MCAD
R845	T1251	T1244	conj	MCAD,MCAD
R846	T1252	T1251	punct	−,MCAD
R847	T1253	T1251	punct	/,MCAD
R848	T1254	T1251	punct	−,MCAD
R849	T1255	T1243	prep	on,produce
R85	T199	T197	amod	deficient,mouse
R850	T1256	T1257	det	a,background
R851	T1257	T1255	pobj	background,on
R852	T1258	T1257	nmod	B6,background
R853	T1259	T1258	punct	/,B6
R854	T1260	T1258	nummod	129,B6
R855	T1261	T1257	amod	mixed,background
R856	T1262	T1233	punct	.,backcrossed
R857	T1264	T1265	det	The,studies
R858	T1265	T1266	nsubjpass	studies,conducted
R859	T1267	T1265	acl	described,studies
R86	T200	T199	punct	-,deficient
R860	T1268	T1267	advmod	here,described
R861	T1269	T1266	auxpass	were,conducted
R862	T1270	T1266	advmod	exclusively,conducted
R863	T1271	T1266	prep	on,conducted
R864	T1272	T1273	det	the,background
R865	T1273	T1271	pobj	background,on
R866	T1274	T1273	nmod	B6,background
R867	T1275	T1274	punct	/,B6
R868	T1276	T1274	nummod	129,B6
R869	T1277	T1273	amod	mixed,background
R87	T202	T203	amod	important,aspects
R870	T1278	T1273	prep	compared,background
R871	T1279	T1278	prep	with,compared
R872	T1280	T1281	compound	littermate,controls
R873	T1281	T1279	pobj	controls,with
R874	T1282	T1281	cc	or,controls
R875	T1283	T1284	nmod	B6,groups
R876	T1284	T1281	conj	groups,controls
R877	T1285	T1283	punct	/,B6
R878	T1286	T1283	nummod	129,B6
R879	T1287	T1284	compound	control,groups
R88	T203	T201	dobj	aspects,reproduced
R880	T1288	T1281	acl	maintained,controls
R881	T1289	T1288	prep	by,maintained
R882	T1290	T1289	pobj	intercrosses,by
R883	T1291	T1292	mark	as,were
R884	T1292	T1288	advcl	were,maintained
R885	T1293	T1294	det	the,mutants
R886	T1294	T1292	nsubj	mutants,were
R887	T1295	T1266	punct	.,conducted
R888	T1297	T1298	csubj	Perpetuating,proved
R889	T1299	T1300	det	this,mutation
R89	T204	T203	prep	of,aspects
R890	T1300	T1297	dobj	mutation,Perpetuating
R891	T1301	T1297	prep	as,Perpetuating
R892	T1302	T1303	det	a,line
R893	T1303	T1301	pobj	line,as
R894	T1304	T1303	amod	congenic,line
R895	T1305	T1303	compound	mutant,line
R896	T1306	T1303	prep	on,line
R897	T1307	T1308	det	the,background
R898	T1308	T1306	pobj	background,on
R899	T1309	T1308	compound	129P2,background
R9	T87	T89	advcl	understand,developed
R90	T205	T206	amod	human,deficiency
R900	T1310	T1298	oprd	impractical,proved
R901	T1311	T1298	punct	.,proved
R902	T1313	T1314	det	The,mice
R903	T1314	T1316	nsubj	mice,were
R904	T1315	T1314	compound	129P2,mice
R905	T1317	T1318	amod	poor,breeders
R906	T1318	T1316	attr	breeders,were
R907	T1319	T1316	prep	as,were
R908	T1320	T1321	amod	wild,types
R909	T1321	T1319	pobj	types,as
R91	T206	T204	pobj	deficiency,of
R910	T1322	T1321	punct	-,types
R911	T1323	T1316	punct	", ",were
R912	T1324	T1316	cc	and,were
R913	T1325	T1326	advmod	when,introduced
R914	T1326	T1327	advcl	introduced,lost
R915	T1327	T1316	conj	lost,were
R916	T1328	T1327	punct	", ",lost
R917	T1329	T1330	det	the,mutation
R918	T1330	T1327	nsubjpass	mutation,lost
R919	T1331	T1330	compound	Acadm,mutation
R92	T207	T206	compound	MCAD,deficiency
R920	T1332	T1327	auxpass	was,lost
R921	T1333	T1327	advmod	nearly,lost
R922	T1334	T1327	prep	on,lost
R923	T1335	T1336	det	this,background
R924	T1336	T1334	pobj	background,on
R925	T1337	T1327	prep	because,lost
R926	T1338	T1337	pcomp	of,because
R927	T1339	T1340	det	the,rate
R928	T1340	T1337	pobj	rate,because
R929	T1341	T1340	amod	high,rate
R93	T208	T201	cc	and,reproduced
R930	T1342	T1340	prep	of,rate
R931	T1343	T1344	amod	neonatal,death
R932	T1344	T1342	pobj	death,of
R933	T1345	T1327	punct	.,lost
R934	T1347	T1348	prep	Because,proved
R935	T1349	T1347	pcomp	of,Because
R936	T1350	T1351	det	the,structure
R937	T1351	T1347	pobj	structure,Because
R938	T1352	T1351	amod	molecular,structure
R939	T1353	T1351	prep	of,structure
R94	T209	T201	conj	is,reproduced
R940	T1354	T1355	det	the,allele
R941	T1355	T1353	pobj	allele,of
R942	T1356	T1355	amod	targeted,allele
R943	T1357	T1348	punct	", ",proved
R944	T1358	T1348	nsubj	it,proved
R945	T1359	T1360	advmod	virtually,impossible
R946	T1360	T1348	oprd	impossible,proved
R947	T1361	T1362	aux	to,distinguish
R948	T1362	T1348	xcomp	distinguish,proved
R949	T1363	T1364	det	all,genotypes
R95	T210	T211	det	a,model
R950	T1364	T1362	dobj	genotypes,distinguish
R951	T1365	T1364	nummod	three,genotypes
R952	T1366	T1364	amod	potential,genotypes
R953	T1367	T1348	punct	.,proved
R954	T1369	T1370	nsubj	We,detect
R955	T1370	T1373	ccomp	detect,determined
R956	T1371	T1370	aux	could,detect
R957	T1372	T1370	advmod	clearly,detect
R958	T1374	T1375	det	the,presence
R959	T1375	T1370	dobj	presence,detect
R96	T211	T209	attr	model,is
R960	T1376	T1375	cc	or,presence
R961	T1377	T1375	conj	absence,presence
R962	T1378	T1375	prep	of,presence
R963	T1379	T1380	det	the,allele
R964	T1380	T1378	pobj	allele,of
R965	T1381	T1380	amod	targeted,allele
R966	T1382	T1373	punct	", ",determined
R967	T1383	T1373	advmod	however,determined
R968	T1384	T1373	punct	", ",determined
R969	T1385	T1386	mark	whether,was
R97	T212	T211	amod	valuable,model
R970	T1386	T1373	csubjpass	was,determined
R971	T1387	T1388	det	a,mouse
R972	T1388	T1386	nsubj	mouse,was
R973	T1389	T1388	amod	particular,mouse
R974	T1390	T1386	attr	MCAD,was
R975	T1391	T1390	punct	−,MCAD
R976	T1392	T1390	punct	/,MCAD
R977	T1393	T1390	punct	−,MCAD
R978	T1394	T1390	cc	or,MCAD
R979	T1395	T1390	conj	MCAD,MCAD
R98	T213	T211	prep	for,model
R980	T1396	T1395	punct	+,MCAD
R981	T1397	T1395	punct	/,MCAD
R982	T1398	T1395	punct	−,MCAD
R983	T1399	T1373	aux	could,determined
R984	T1400	T1373	neg	not,determined
R985	T1401	T1373	auxpass	be,determined
R986	T1402	T1373	prep	by,determined
R987	T1403	T1404	compound	Southern,blot
R988	T1404	T1402	pobj	blot,by
R989	T1405	T1404	cc	or,blot
R99	T214	T215	amod	further,analysis
R990	T1406	T1404	conj	PCR,blot
R991	T1407	T1404	prep	of,blot
R992	T1408	T1409	amod	genomic,DNA
R993	T1409	T1407	pobj	DNA,of
R994	T1410	T1373	punct	.,determined
R995	T1412	T1413	advmod	Ultimately,ascertained
R996	T1414	T1415	nmod	MCAD,mice
R997	T1415	T1413	nsubjpass	mice,ascertained
R998	T1416	T1414	punct	−,MCAD
R999	T1417	T1414	punct	/,MCAD

PMC:1189074 JSONTXT 8 Projects

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PMC:1189074 JSON TXT 8 Projects