| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T20424 |
0-19 |
NN |
denotes |
Immunocytochemistry |
| T20425 |
20-22 |
IN |
denotes |
on |
| T20426 |
23-27 |
NN |
denotes |
MDCK |
| T20427 |
28-33 |
NNS |
denotes |
cells |
| T20428 |
33-34 |
. |
denotes |
. |
| T20429 |
34-245 |
sentence |
denotes |
MDCK stable cell lines expressing vector alone, wild-type AQP2, or AQP2-F204V (and in some cases transiently expressing a GFP construct) were grown on fibronectin-coated coverslips until tight junctions formed. |
| T20430 |
35-39 |
NN |
denotes |
MDCK |
| T20432 |
40-46 |
JJ |
denotes |
stable |
| T20433 |
47-51 |
NN |
denotes |
cell |
| T20431 |
52-57 |
NNS |
denotes |
lines |
| T20435 |
58-68 |
VBG |
denotes |
expressing |
| T20436 |
69-75 |
NN |
denotes |
vector |
| T20437 |
76-81 |
JJ |
denotes |
alone |
| T20438 |
81-83 |
, |
denotes |
, |
| T20439 |
83-87 |
JJ |
denotes |
wild |
| T20441 |
87-88 |
HYPH |
denotes |
- |
| T20440 |
88-92 |
NN |
denotes |
type |
| T20442 |
93-97 |
NN |
denotes |
AQP2 |
| T20443 |
97-99 |
, |
denotes |
, |
| T20444 |
99-101 |
CC |
denotes |
or |
| T20445 |
102-106 |
NN |
denotes |
AQP2 |
| T20447 |
106-107 |
HYPH |
denotes |
- |
| T20446 |
107-112 |
NN |
denotes |
F204V |
| T20448 |
113-114 |
-LRB- |
denotes |
( |
| T20449 |
114-117 |
CC |
denotes |
and |
| T20450 |
118-120 |
IN |
denotes |
in |
| T20452 |
121-125 |
DT |
denotes |
some |
| T20453 |
126-131 |
NNS |
denotes |
cases |
| T20454 |
132-143 |
RB |
denotes |
transiently |
| T20451 |
144-154 |
VBG |
denotes |
expressing |
| T20455 |
155-156 |
DT |
denotes |
a |
| T20457 |
157-160 |
NN |
denotes |
GFP |
| T20456 |
161-170 |
NN |
denotes |
construct |
| T20458 |
170-171 |
-RRB- |
denotes |
) |
| T20459 |
172-176 |
VBD |
denotes |
were |
| T20434 |
177-182 |
VBN |
denotes |
grown |
| T20460 |
183-185 |
IN |
denotes |
on |
| T20461 |
186-197 |
NN |
denotes |
fibronectin |
| T20463 |
197-198 |
HYPH |
denotes |
- |
| T20462 |
198-204 |
VBN |
denotes |
coated |
| T20464 |
205-215 |
NNS |
denotes |
coverslips |
| T20465 |
216-221 |
IN |
denotes |
until |
| T20467 |
222-227 |
JJ |
denotes |
tight |
| T20468 |
228-237 |
NNS |
denotes |
junctions |
| T20466 |
238-244 |
VBD |
denotes |
formed |
| T20469 |
244-245 |
. |
denotes |
. |
| T20470 |
245-342 |
sentence |
denotes |
Cells were treated with or without 150 μM forskolin for 90 min, and fixed in methanol at −20 °C. |
| T20471 |
246-251 |
NNS |
denotes |
Cells |
| T20473 |
252-256 |
VBD |
denotes |
were |
| T20472 |
257-264 |
VBN |
denotes |
treated |
| T20474 |
265-269 |
IN |
denotes |
with |
| T20475 |
270-272 |
CC |
denotes |
or |
| T20476 |
273-280 |
IN |
denotes |
without |
| T20477 |
281-284 |
CD |
denotes |
150 |
| T20478 |
285-287 |
NN |
denotes |
μM |
| T20479 |
288-297 |
NN |
denotes |
forskolin |
| T20480 |
298-301 |
IN |
denotes |
for |
| T20481 |
302-304 |
CD |
denotes |
90 |
| T20482 |
305-308 |
NN |
denotes |
min |
| T20483 |
308-310 |
, |
denotes |
, |
| T20484 |
310-313 |
CC |
denotes |
and |
| T20485 |
314-319 |
VBN |
denotes |
fixed |
| T20486 |
320-322 |
IN |
denotes |
in |
| T20487 |
323-331 |
NN |
denotes |
methanol |
| T20488 |
332-334 |
IN |
denotes |
at |
| T20489 |
335-336 |
SYM |
denotes |
− |
| T20490 |
336-338 |
CD |
denotes |
20 |
| T20491 |
339-341 |
NN |
denotes |
°C |
| T20492 |
341-342 |
. |
denotes |
. |
| T20493 |
342-508 |
sentence |
denotes |
Subsequently, cells were washed and permeabilized in 0.2% Triton X-100 for 5 min, and sequentially probed for AQP2 and organelle markers for either the PM or the ER. |
| T20494 |
343-355 |
RB |
denotes |
Subsequently |
| T20496 |
355-357 |
, |
denotes |
, |
| T20497 |
357-362 |
NNS |
denotes |
cells |
| T20498 |
363-367 |
VBD |
denotes |
were |
| T20495 |
368-374 |
VBN |
denotes |
washed |
| T20499 |
375-378 |
CC |
denotes |
and |
| T20500 |
379-392 |
VBN |
denotes |
permeabilized |
| T20501 |
393-395 |
IN |
denotes |
in |
| T20502 |
396-399 |
CD |
denotes |
0.2 |
| T20503 |
399-400 |
NN |
denotes |
% |
| T20505 |
401-407 |
NN |
denotes |
Triton |
| T20504 |
408-409 |
NN |
denotes |
X |
| T20506 |
409-410 |
HYPH |
denotes |
- |
| T20507 |
410-413 |
CD |
denotes |
100 |
| T20508 |
414-417 |
IN |
denotes |
for |
| T20509 |
418-419 |
CD |
denotes |
5 |
| T20510 |
420-423 |
NN |
denotes |
min |
| T20511 |
423-425 |
, |
denotes |
, |
| T20512 |
425-428 |
CC |
denotes |
and |
| T20513 |
429-441 |
RB |
denotes |
sequentially |
| T20514 |
442-448 |
VBN |
denotes |
probed |
| T20515 |
449-452 |
IN |
denotes |
for |
| T20516 |
453-457 |
NN |
denotes |
AQP2 |
| T20517 |
458-461 |
CC |
denotes |
and |
| T20518 |
462-471 |
NN |
denotes |
organelle |
| T20519 |
472-479 |
NNS |
denotes |
markers |
| T20520 |
480-483 |
IN |
denotes |
for |
| T20521 |
484-490 |
CC |
denotes |
either |
| T20523 |
491-494 |
DT |
denotes |
the |
| T20522 |
495-497 |
NN |
denotes |
PM |
| T20524 |
498-500 |
CC |
denotes |
or |
| T20525 |
501-504 |
DT |
denotes |
the |
| T20526 |
505-507 |
NN |
denotes |
ER |
| T20527 |
507-508 |
. |
denotes |
. |
| T20528 |
508-678 |
sentence |
denotes |
AQP2 was detected using goat anti-AQP2 (1:100; Santa Cruz Biotechnology; #sc-9882) and a 1:200 dilution of AlexaFluor 488-conjugated donkey anti-goat secondary antibody. |
| T20529 |
509-513 |
NN |
denotes |
AQP2 |
| T20531 |
514-517 |
VBD |
denotes |
was |
| T20530 |
518-526 |
VBN |
denotes |
detected |
| T20532 |
527-532 |
VBG |
denotes |
using |
| T20533 |
533-537 |
NN |
denotes |
goat |
| T20534 |
538-547 |
JJ |
denotes |
anti-AQP2 |
| T20535 |
548-549 |
-LRB- |
denotes |
( |
| T20537 |
549-550 |
CD |
denotes |
1 |
| T20538 |
550-551 |
SYM |
denotes |
: |
| T20539 |
551-554 |
CD |
denotes |
100 |
| T20540 |
554-555 |
: |
denotes |
; |
| T20541 |
556-561 |
NNP |
denotes |
Santa |
| T20542 |
562-566 |
NNP |
denotes |
Cruz |
| T20543 |
567-580 |
NNP |
denotes |
Biotechnology |
| T20544 |
580-581 |
: |
denotes |
; |
| T20545 |
582-583 |
SYM |
denotes |
# |
| T20536 |
583-585 |
NN |
denotes |
sc |
| T20546 |
585-586 |
HYPH |
denotes |
- |
| T20547 |
586-590 |
CD |
denotes |
9882 |
| T20548 |
590-591 |
-RRB- |
denotes |
) |
| T20549 |
592-595 |
CC |
denotes |
and |
| T20550 |
596-597 |
DT |
denotes |
a |
| T20552 |
598-599 |
CD |
denotes |
1 |
| T20554 |
599-600 |
SYM |
denotes |
: |
| T20553 |
600-603 |
CD |
denotes |
200 |
| T20551 |
604-612 |
NN |
denotes |
dilution |
| T20555 |
613-615 |
IN |
denotes |
of |
| T20556 |
616-626 |
NNP |
denotes |
AlexaFluor |
| T20558 |
627-630 |
CD |
denotes |
488 |
| T20559 |
630-631 |
HYPH |
denotes |
- |
| T20557 |
631-641 |
VBN |
denotes |
conjugated |
| T20561 |
642-648 |
NN |
denotes |
donkey |
| T20562 |
649-658 |
JJ |
denotes |
anti-goat |
| T20563 |
659-668 |
JJ |
denotes |
secondary |
| T20560 |
669-677 |
NN |
denotes |
antibody |
| T20564 |
677-678 |
. |
denotes |
. |
| T20565 |
678-1086 |
sentence |
denotes |
The PM and ER were probed using mouse anti-Na+/K+-ATPase (Upstate, Waltham, Massachusetts, United States) or rabbit anti-calnexin (Stressgen Biotechnology, Victoria, British Columbia, Canada) antibodies and the secondary antibodies, Cy3-conjugated goat anti-mouse (1:200; Jackson ImmunoResearch, West Grove, Pennsylvania, United States) or AlexaFluor 594-conjugated chicken anti-rabbit (1:200) respectively. |
| T20566 |
679-682 |
DT |
denotes |
The |
| T20567 |
683-685 |
NN |
denotes |
PM |
| T20569 |
686-689 |
CC |
denotes |
and |
| T20570 |
690-692 |
NN |
denotes |
ER |
| T20571 |
693-697 |
VBD |
denotes |
were |
| T20568 |
698-704 |
VBN |
denotes |
probed |
| T20572 |
705-710 |
VBG |
denotes |
using |
| T20573 |
711-716 |
NN |
denotes |
mouse |
| T20575 |
717-725 |
JJ |
denotes |
anti-Na+ |
| T20576 |
725-726 |
HYPH |
denotes |
/ |
| T20577 |
726-728 |
NN |
denotes |
K+ |
| T20578 |
728-729 |
HYPH |
denotes |
- |
| T20574 |
729-735 |
NN |
denotes |
ATPase |
| T20580 |
736-737 |
-LRB- |
denotes |
( |
| T20581 |
737-744 |
NNP |
denotes |
Upstate |
| T20582 |
744-746 |
, |
denotes |
, |
| T20583 |
746-753 |
NNP |
denotes |
Waltham |
| T20584 |
753-755 |
, |
denotes |
, |
| T20585 |
755-768 |
NNP |
denotes |
Massachusetts |
| T20586 |
768-770 |
, |
denotes |
, |
| T20587 |
770-776 |
NNP |
denotes |
United |
| T20588 |
777-783 |
NNP |
denotes |
States |
| T20589 |
783-784 |
-RRB- |
denotes |
) |
| T20590 |
785-787 |
CC |
denotes |
or |
| T20591 |
788-794 |
NN |
denotes |
rabbit |
| T20592 |
795-808 |
JJ |
denotes |
anti-calnexin |
| T20593 |
809-810 |
-LRB- |
denotes |
( |
| T20595 |
810-819 |
NNP |
denotes |
Stressgen |
| T20594 |
820-833 |
NNP |
denotes |
Biotechnology |
| T20596 |
833-835 |
, |
denotes |
, |
| T20597 |
835-843 |
NNP |
denotes |
Victoria |
| T20598 |
843-845 |
, |
denotes |
, |
| T20599 |
845-852 |
NNP |
denotes |
British |
| T20600 |
853-861 |
NNP |
denotes |
Columbia |
| T20601 |
861-863 |
, |
denotes |
, |
| T20602 |
863-869 |
NNP |
denotes |
Canada |
| T20603 |
869-870 |
-RRB- |
denotes |
) |
| T20579 |
871-881 |
NNS |
denotes |
antibodies |
| T20604 |
882-885 |
CC |
denotes |
and |
| T20605 |
886-889 |
DT |
denotes |
the |
| T20607 |
890-899 |
JJ |
denotes |
secondary |
| T20606 |
900-910 |
NNS |
denotes |
antibodies |
| T20608 |
910-912 |
, |
denotes |
, |
| T20609 |
912-915 |
NN |
denotes |
Cy3 |
| T20611 |
915-916 |
HYPH |
denotes |
- |
| T20610 |
916-926 |
VBN |
denotes |
conjugated |
| T20612 |
927-931 |
NN |
denotes |
goat |
| T20613 |
932-942 |
JJ |
denotes |
anti-mouse |
| T20614 |
943-944 |
-LRB- |
denotes |
( |
| T20616 |
944-945 |
CD |
denotes |
1 |
| T20617 |
945-946 |
SYM |
denotes |
: |
| T20618 |
946-949 |
CD |
denotes |
200 |
| T20619 |
949-950 |
: |
denotes |
; |
| T20620 |
951-958 |
NNP |
denotes |
Jackson |
| T20615 |
959-973 |
NNP |
denotes |
ImmunoResearch |
| T20621 |
973-975 |
, |
denotes |
, |
| T20622 |
975-979 |
NNP |
denotes |
West |
| T20623 |
980-985 |
NNP |
denotes |
Grove |
| T20624 |
985-987 |
, |
denotes |
, |
| T20625 |
987-999 |
NNP |
denotes |
Pennsylvania |
| T20626 |
999-1001 |
, |
denotes |
, |
| T20627 |
1001-1007 |
NNP |
denotes |
United |
| T20628 |
1008-1014 |
NNP |
denotes |
States |
| T20629 |
1014-1015 |
-RRB- |
denotes |
) |
| T20630 |
1016-1018 |
CC |
denotes |
or |
| T20631 |
1019-1029 |
NNP |
denotes |
AlexaFluor |
| T20633 |
1030-1033 |
CD |
denotes |
594 |
| T20634 |
1033-1034 |
HYPH |
denotes |
- |
| T20632 |
1034-1044 |
VBN |
denotes |
conjugated |
| T20635 |
1045-1052 |
NN |
denotes |
chicken |
| T20636 |
1053-1064 |
JJ |
denotes |
anti-rabbit |
| T20637 |
1065-1066 |
-LRB- |
denotes |
( |
| T20638 |
1066-1067 |
CD |
denotes |
1 |
| T20639 |
1067-1068 |
SYM |
denotes |
: |
| T20640 |
1068-1071 |
CD |
denotes |
200 |
| T20641 |
1071-1072 |
-RRB- |
denotes |
) |
| T20642 |
1073-1085 |
RB |
denotes |
respectively |
| T20643 |
1085-1086 |
. |
denotes |
. |
| T20644 |
1086-1166 |
sentence |
denotes |
Cells were washed in PBS, counterstained with DAPI, and mounted in Vectashield. |
| T20645 |
1087-1092 |
NNS |
denotes |
Cells |
| T20647 |
1093-1097 |
VBD |
denotes |
were |
| T20646 |
1098-1104 |
VBN |
denotes |
washed |
| T20648 |
1105-1107 |
IN |
denotes |
in |
| T20649 |
1108-1111 |
NN |
denotes |
PBS |
| T20650 |
1111-1113 |
, |
denotes |
, |
| T20651 |
1113-1127 |
VBN |
denotes |
counterstained |
| T20652 |
1128-1132 |
IN |
denotes |
with |
| T20653 |
1133-1137 |
NN |
denotes |
DAPI |
| T20654 |
1137-1139 |
, |
denotes |
, |
| T20655 |
1139-1142 |
CC |
denotes |
and |
| T20656 |
1143-1150 |
VBN |
denotes |
mounted |
| T20657 |
1151-1153 |
IN |
denotes |
in |
| T20658 |
1154-1165 |
NNP |
denotes |
Vectashield |
| T20659 |
1165-1166 |
. |
denotes |
. |
| T20660 |
1166-1384 |
sentence |
denotes |
In experiments in which GFP fusions were used, AQP2 was probed using the antibody combination used for kidney immunohistochemistry in order to detect the AQP2 at 594 nm, to distinguish between the GFP fusion proteins. |
| T20661 |
1167-1169 |
IN |
denotes |
In |
| T20663 |
1170-1181 |
NNS |
denotes |
experiments |
| T20664 |
1182-1184 |
IN |
denotes |
in |
| T20666 |
1185-1190 |
WDT |
denotes |
which |
| T20667 |
1191-1194 |
NN |
denotes |
GFP |
| T20668 |
1195-1202 |
NNS |
denotes |
fusions |
| T20669 |
1203-1207 |
VBD |
denotes |
were |
| T20665 |
1208-1212 |
VBN |
denotes |
used |
| T20670 |
1212-1214 |
, |
denotes |
, |
| T20671 |
1214-1218 |
NN |
denotes |
AQP2 |
| T20672 |
1219-1222 |
VBD |
denotes |
was |
| T20662 |
1223-1229 |
VBN |
denotes |
probed |
| T20673 |
1230-1235 |
VBG |
denotes |
using |
| T20674 |
1236-1239 |
DT |
denotes |
the |
| T20676 |
1240-1248 |
NN |
denotes |
antibody |
| T20675 |
1249-1260 |
NN |
denotes |
combination |
| T20677 |
1261-1265 |
VBN |
denotes |
used |
| T20678 |
1266-1269 |
IN |
denotes |
for |
| T20679 |
1270-1276 |
NN |
denotes |
kidney |
| T20680 |
1277-1297 |
NN |
denotes |
immunohistochemistry |
| T20681 |
1298-1300 |
IN |
denotes |
in |
| T20682 |
1301-1306 |
NN |
denotes |
order |
| T20683 |
1307-1309 |
TO |
denotes |
to |
| T20684 |
1310-1316 |
VB |
denotes |
detect |
| T20685 |
1317-1320 |
DT |
denotes |
the |
| T20686 |
1321-1325 |
NN |
denotes |
AQP2 |
| T20687 |
1326-1328 |
IN |
denotes |
at |
| T20688 |
1329-1332 |
CD |
denotes |
594 |
| T20689 |
1333-1335 |
NN |
denotes |
nm |
| T20690 |
1335-1337 |
, |
denotes |
, |
| T20691 |
1337-1339 |
TO |
denotes |
to |
| T20692 |
1340-1351 |
VB |
denotes |
distinguish |
| T20693 |
1352-1359 |
IN |
denotes |
between |
| T20694 |
1360-1363 |
DT |
denotes |
the |
| T20696 |
1364-1367 |
NN |
denotes |
GFP |
| T20697 |
1368-1374 |
NN |
denotes |
fusion |
| T20695 |
1375-1383 |
NN |
denotes |
proteins |
| T20698 |
1383-1384 |
. |
denotes |
. |
| R5833 |
T20425 |
T20424 |
prep |
on,Immunocytochemistry |
| R5834 |
T20426 |
T20427 |
compound |
MDCK,cells |
| R5835 |
T20427 |
T20425 |
pobj |
cells,on |
| R5836 |
T20428 |
T20424 |
punct |
.,Immunocytochemistry |
| R5837 |
T20430 |
T20431 |
nmod |
MDCK,lines |
| R5838 |
T20431 |
T20434 |
nsubjpass |
lines,grown |
| R5839 |
T20432 |
T20431 |
amod |
stable,lines |
| R5840 |
T20433 |
T20431 |
compound |
cell,lines |
| R5841 |
T20435 |
T20431 |
acl |
expressing,lines |
| R5842 |
T20436 |
T20435 |
dobj |
vector,expressing |
| R5843 |
T20437 |
T20436 |
amod |
alone,vector |
| R5844 |
T20438 |
T20436 |
punct |
", ",vector |
| R5845 |
T20439 |
T20440 |
amod |
wild,type |
| R5846 |
T20440 |
T20442 |
compound |
type,AQP2 |
| R5847 |
T20441 |
T20440 |
punct |
-,type |
| R5848 |
T20442 |
T20436 |
conj |
AQP2,vector |
| R5849 |
T20443 |
T20442 |
punct |
", ",AQP2 |
| R5850 |
T20444 |
T20442 |
cc |
or,AQP2 |
| R5851 |
T20445 |
T20446 |
compound |
AQP2,F204V |
| R5852 |
T20446 |
T20442 |
conj |
F204V,AQP2 |
| R5853 |
T20447 |
T20446 |
punct |
-,F204V |
| R5854 |
T20448 |
T20435 |
punct |
(,expressing |
| R5855 |
T20449 |
T20435 |
cc |
and,expressing |
| R5856 |
T20450 |
T20451 |
prep |
in,expressing |
| R5857 |
T20451 |
T20435 |
conj |
expressing,expressing |
| R5858 |
T20452 |
T20453 |
det |
some,cases |
| R5859 |
T20453 |
T20450 |
pobj |
cases,in |
| R5860 |
T20454 |
T20451 |
advmod |
transiently,expressing |
| R5861 |
T20455 |
T20456 |
det |
a,construct |
| R5862 |
T20456 |
T20451 |
dobj |
construct,expressing |
| R5863 |
T20457 |
T20456 |
compound |
GFP,construct |
| R5864 |
T20458 |
T20451 |
punct |
),expressing |
| R5865 |
T20459 |
T20434 |
auxpass |
were,grown |
| R5866 |
T20460 |
T20434 |
prep |
on,grown |
| R5867 |
T20461 |
T20462 |
npadvmod |
fibronectin,coated |
| R5868 |
T20462 |
T20464 |
amod |
coated,coverslips |
| R5869 |
T20463 |
T20462 |
punct |
-,coated |
| R5870 |
T20464 |
T20460 |
pobj |
coverslips,on |
| R5871 |
T20465 |
T20466 |
mark |
until,formed |
| R5872 |
T20466 |
T20434 |
advcl |
formed,grown |
| R5873 |
T20467 |
T20468 |
amod |
tight,junctions |
| R5874 |
T20468 |
T20466 |
nsubj |
junctions,formed |
| R5875 |
T20469 |
T20434 |
punct |
.,grown |
| R5876 |
T20471 |
T20472 |
nsubjpass |
Cells,treated |
| R5877 |
T20473 |
T20472 |
auxpass |
were,treated |
| R5878 |
T20474 |
T20472 |
prep |
with,treated |
| R5879 |
T20475 |
T20474 |
cc |
or,with |
| R5880 |
T20476 |
T20474 |
conj |
without,with |
| R5881 |
T20477 |
T20478 |
nummod |
150,μM |
| R5882 |
T20478 |
T20479 |
compound |
μM,forskolin |
| R5883 |
T20479 |
T20476 |
pobj |
forskolin,without |
| R5884 |
T20480 |
T20472 |
prep |
for,treated |
| R5885 |
T20481 |
T20482 |
nummod |
90,min |
| R5886 |
T20482 |
T20480 |
pobj |
min,for |
| R5887 |
T20483 |
T20472 |
punct |
", ",treated |
| R5888 |
T20484 |
T20472 |
cc |
and,treated |
| R5889 |
T20485 |
T20472 |
conj |
fixed,treated |
| R5890 |
T20486 |
T20485 |
prep |
in,fixed |
| R5891 |
T20487 |
T20486 |
pobj |
methanol,in |
| R5892 |
T20488 |
T20485 |
prep |
at,fixed |
| R5893 |
T20489 |
T20490 |
punct |
−,20 |
| R5894 |
T20490 |
T20491 |
nummod |
20,°C |
| R5895 |
T20491 |
T20488 |
pobj |
°C,at |
| R5896 |
T20492 |
T20472 |
punct |
.,treated |
| R5897 |
T20494 |
T20495 |
advmod |
Subsequently,washed |
| R5898 |
T20496 |
T20495 |
punct |
", ",washed |
| R5899 |
T20497 |
T20495 |
nsubj |
cells,washed |
| R5900 |
T20498 |
T20495 |
aux |
were,washed |
| R5901 |
T20499 |
T20495 |
cc |
and,washed |
| R5902 |
T20500 |
T20495 |
conj |
permeabilized,washed |
| R5903 |
T20501 |
T20500 |
prep |
in,permeabilized |
| R5904 |
T20502 |
T20503 |
nummod |
0.2,% |
| R5905 |
T20503 |
T20504 |
compound |
%,X |
| R5906 |
T20504 |
T20501 |
pobj |
X,in |
| R5907 |
T20505 |
T20504 |
compound |
Triton,X |
| R5908 |
T20506 |
T20504 |
punct |
-,X |
| R5909 |
T20507 |
T20504 |
nummod |
100,X |
| R5910 |
T20508 |
T20500 |
prep |
for,permeabilized |
| R5911 |
T20509 |
T20510 |
nummod |
5,min |
| R5912 |
T20510 |
T20508 |
pobj |
min,for |
| R5913 |
T20511 |
T20495 |
punct |
", ",washed |
| R5914 |
T20512 |
T20495 |
cc |
and,washed |
| R5915 |
T20513 |
T20514 |
advmod |
sequentially,probed |
| R5916 |
T20514 |
T20495 |
conj |
probed,washed |
| R5917 |
T20515 |
T20514 |
prep |
for,probed |
| R5918 |
T20516 |
T20515 |
pobj |
AQP2,for |
| R5919 |
T20517 |
T20516 |
cc |
and,AQP2 |
| R5920 |
T20518 |
T20519 |
compound |
organelle,markers |
| R5921 |
T20519 |
T20516 |
conj |
markers,AQP2 |
| R5922 |
T20520 |
T20519 |
prep |
for,markers |
| R5923 |
T20521 |
T20522 |
preconj |
either,PM |
| R5924 |
T20522 |
T20520 |
pobj |
PM,for |
| R5925 |
T20523 |
T20522 |
det |
the,PM |
| R5926 |
T20524 |
T20522 |
cc |
or,PM |
| R5927 |
T20525 |
T20526 |
det |
the,ER |
| R5928 |
T20526 |
T20522 |
conj |
ER,PM |
| R5929 |
T20527 |
T20495 |
punct |
.,washed |
| R5930 |
T20529 |
T20530 |
nsubjpass |
AQP2,detected |
| R5931 |
T20531 |
T20530 |
auxpass |
was,detected |
| R5932 |
T20532 |
T20530 |
advcl |
using,detected |
| R5933 |
T20533 |
T20532 |
dobj |
goat,using |
| R5934 |
T20534 |
T20533 |
amod |
anti-AQP2,goat |
| R5935 |
T20535 |
T20536 |
punct |
(,sc |
| R5936 |
T20536 |
T20533 |
parataxis |
sc,goat |
| R5937 |
T20537 |
T20536 |
dep |
1,sc |
| R5938 |
T20538 |
T20539 |
punct |
:,100 |
| R5939 |
T20539 |
T20537 |
prep |
100,1 |
| R5940 |
T20540 |
T20536 |
punct |
;,sc |
| R5941 |
T20541 |
T20542 |
compound |
Santa,Cruz |
| R5942 |
T20542 |
T20543 |
compound |
Cruz,Biotechnology |
| R5943 |
T20543 |
T20536 |
dep |
Biotechnology,sc |
| R5944 |
T20544 |
T20536 |
punct |
;,sc |
| R5945 |
T20545 |
T20536 |
punct |
#,sc |
| R5946 |
T20546 |
T20536 |
punct |
-,sc |
| R5947 |
T20547 |
T20536 |
nummod |
9882,sc |
| R5948 |
T20548 |
T20536 |
punct |
),sc |
| R5949 |
T20549 |
T20533 |
cc |
and,goat |
| R5950 |
T20550 |
T20551 |
det |
a,dilution |
| R5951 |
T20551 |
T20533 |
conj |
dilution,goat |
| R5952 |
T20552 |
T20553 |
quantmod |
1,200 |
| R5953 |
T20553 |
T20551 |
nummod |
200,dilution |
| R5954 |
T20554 |
T20553 |
punct |
:,200 |
| R5955 |
T20555 |
T20551 |
prep |
of,dilution |
| R5956 |
T20556 |
T20557 |
npadvmod |
AlexaFluor,conjugated |
| R5957 |
T20557 |
T20560 |
amod |
conjugated,antibody |
| R5958 |
T20558 |
T20556 |
nummod |
488,AlexaFluor |
| R5959 |
T20559 |
T20557 |
punct |
-,conjugated |
| R5960 |
T20560 |
T20555 |
pobj |
antibody,of |
| R5961 |
T20561 |
T20560 |
nmod |
donkey,antibody |
| R5962 |
T20562 |
T20560 |
amod |
anti-goat,antibody |
| R5963 |
T20563 |
T20560 |
amod |
secondary,antibody |
| R5964 |
T20564 |
T20530 |
punct |
.,detected |
| R5965 |
T20566 |
T20567 |
det |
The,PM |
| R5966 |
T20567 |
T20568 |
nsubjpass |
PM,probed |
| R5967 |
T20569 |
T20567 |
cc |
and,PM |
| R5968 |
T20570 |
T20567 |
conj |
ER,PM |
| R5969 |
T20571 |
T20568 |
auxpass |
were,probed |
| R5970 |
T20572 |
T20568 |
advcl |
using,probed |
| R5971 |
T20573 |
T20574 |
nmod |
mouse,ATPase |
| R5972 |
T20574 |
T20579 |
nmod |
ATPase,antibodies |
| R5973 |
T20575 |
T20574 |
amod |
anti-Na+,ATPase |
| R5974 |
T20576 |
T20574 |
punct |
/,ATPase |
| R5975 |
T20577 |
T20574 |
nmod |
K+,ATPase |
| R5976 |
T20578 |
T20574 |
punct |
-,ATPase |
| R5977 |
T20579 |
T20572 |
dobj |
antibodies,using |
| R5978 |
T20580 |
T20581 |
punct |
(,Upstate |
| R5979 |
T20581 |
T20574 |
parataxis |
Upstate,ATPase |
| R5980 |
T20582 |
T20581 |
punct |
", ",Upstate |
| R5981 |
T20583 |
T20581 |
npadvmod |
Waltham,Upstate |
| R5982 |
T20584 |
T20581 |
punct |
", ",Upstate |
| R5983 |
T20585 |
T20581 |
npadvmod |
Massachusetts,Upstate |
| R5984 |
T20586 |
T20581 |
punct |
", ",Upstate |
| R5985 |
T20587 |
T20588 |
compound |
United,States |
| R5986 |
T20588 |
T20581 |
npadvmod |
States,Upstate |
| R5987 |
T20589 |
T20581 |
punct |
),Upstate |
| R5988 |
T20590 |
T20574 |
cc |
or,ATPase |
| R5989 |
T20591 |
T20592 |
npadvmod |
rabbit,anti-calnexin |
| R5990 |
T20592 |
T20574 |
conj |
anti-calnexin,ATPase |
| R5991 |
T20593 |
T20594 |
punct |
(,Biotechnology |
| R5992 |
T20594 |
T20592 |
parataxis |
Biotechnology,anti-calnexin |
| R5993 |
T20595 |
T20594 |
compound |
Stressgen,Biotechnology |
| R5994 |
T20596 |
T20594 |
punct |
", ",Biotechnology |
| R5995 |
T20597 |
T20594 |
npadvmod |
Victoria,Biotechnology |
| R5996 |
T20598 |
T20594 |
punct |
", ",Biotechnology |
| R5997 |
T20599 |
T20600 |
compound |
British,Columbia |
| R5998 |
T20600 |
T20594 |
npadvmod |
Columbia,Biotechnology |
| R5999 |
T20601 |
T20594 |
punct |
", ",Biotechnology |
| R6000 |
T20602 |
T20594 |
npadvmod |
Canada,Biotechnology |
| R6001 |
T20603 |
T20594 |
punct |
),Biotechnology |
| R6002 |
T20604 |
T20579 |
cc |
and,antibodies |
| R6003 |
T20605 |
T20606 |
det |
the,antibodies |
| R6004 |
T20606 |
T20579 |
conj |
antibodies,antibodies |
| R6005 |
T20607 |
T20606 |
amod |
secondary,antibodies |
| R6006 |
T20608 |
T20606 |
punct |
", ",antibodies |
| R6007 |
T20609 |
T20610 |
npadvmod |
Cy3,conjugated |
| R6008 |
T20610 |
T20612 |
amod |
conjugated,goat |
| R6009 |
T20611 |
T20610 |
punct |
-,conjugated |
| R6010 |
T20612 |
T20606 |
appos |
goat,antibodies |
| R6011 |
T20613 |
T20612 |
amod |
anti-mouse,goat |
| R6012 |
T20614 |
T20615 |
punct |
(,ImmunoResearch |
| R6013 |
T20615 |
T20612 |
parataxis |
ImmunoResearch,goat |
| R6014 |
T20616 |
T20615 |
dep |
1,ImmunoResearch |
| R6015 |
T20617 |
T20618 |
punct |
:,200 |
| R6016 |
T20618 |
T20616 |
prep |
200,1 |
| R6017 |
T20619 |
T20615 |
punct |
;,ImmunoResearch |
| R6018 |
T20620 |
T20615 |
compound |
Jackson,ImmunoResearch |
| R6019 |
T20621 |
T20615 |
punct |
", ",ImmunoResearch |
| R6020 |
T20622 |
T20623 |
compound |
West,Grove |
| R6021 |
T20623 |
T20615 |
npadvmod |
Grove,ImmunoResearch |
| R6022 |
T20624 |
T20615 |
punct |
", ",ImmunoResearch |
| R6023 |
T20625 |
T20615 |
npadvmod |
Pennsylvania,ImmunoResearch |
| R6024 |
T20626 |
T20615 |
punct |
", ",ImmunoResearch |
| R6025 |
T20627 |
T20628 |
compound |
United,States |
| R6026 |
T20628 |
T20615 |
npadvmod |
States,ImmunoResearch |
| R6027 |
T20629 |
T20615 |
punct |
),ImmunoResearch |
| R6028 |
T20630 |
T20606 |
cc |
or,antibodies |
| R6029 |
T20631 |
T20632 |
npadvmod |
AlexaFluor,conjugated |
| R6030 |
T20632 |
T20635 |
amod |
conjugated,chicken |
| R6031 |
T20633 |
T20631 |
nummod |
594,AlexaFluor |
| R6032 |
T20634 |
T20632 |
punct |
-,conjugated |
| R6033 |
T20635 |
T20606 |
conj |
chicken,antibodies |
| R6034 |
T20636 |
T20635 |
amod |
anti-rabbit,chicken |
| R6035 |
T20637 |
T20638 |
punct |
(,1 |
| R6036 |
T20638 |
T20635 |
parataxis |
1,chicken |
| R6037 |
T20639 |
T20640 |
punct |
:,200 |
| R6038 |
T20640 |
T20638 |
prep |
200,1 |
| R6039 |
T20641 |
T20638 |
punct |
),1 |
| R6040 |
T20642 |
T20572 |
advmod |
respectively,using |
| R6041 |
T20643 |
T20568 |
punct |
.,probed |
| R6042 |
T20645 |
T20646 |
nsubjpass |
Cells,washed |
| R6043 |
T20647 |
T20646 |
auxpass |
were,washed |
| R6044 |
T20648 |
T20646 |
prep |
in,washed |
| R6045 |
T20649 |
T20648 |
pobj |
PBS,in |
| R6046 |
T20650 |
T20646 |
punct |
", ",washed |
| R6047 |
T20651 |
T20646 |
conj |
counterstained,washed |
| R6048 |
T20652 |
T20651 |
prep |
with,counterstained |
| R6049 |
T20653 |
T20652 |
pobj |
DAPI,with |
| R6050 |
T20654 |
T20651 |
punct |
", ",counterstained |
| R6051 |
T20655 |
T20651 |
cc |
and,counterstained |
| R6052 |
T20656 |
T20651 |
conj |
mounted,counterstained |
| R6053 |
T20657 |
T20656 |
prep |
in,mounted |
| R6054 |
T20658 |
T20657 |
pobj |
Vectashield,in |
| R6055 |
T20659 |
T20646 |
punct |
.,washed |
| R6056 |
T20661 |
T20662 |
prep |
In,probed |
| R6057 |
T20663 |
T20661 |
pobj |
experiments,In |
| R6058 |
T20664 |
T20665 |
prep |
in,used |
| R6059 |
T20665 |
T20663 |
relcl |
used,experiments |
| R6060 |
T20666 |
T20664 |
pobj |
which,in |
| R6061 |
T20667 |
T20668 |
compound |
GFP,fusions |
| R6062 |
T20668 |
T20665 |
nsubjpass |
fusions,used |
| R6063 |
T20669 |
T20665 |
auxpass |
were,used |
| R6064 |
T20670 |
T20662 |
punct |
", ",probed |
| R6065 |
T20671 |
T20662 |
nsubjpass |
AQP2,probed |
| R6066 |
T20672 |
T20662 |
auxpass |
was,probed |
| R6067 |
T20673 |
T20662 |
advcl |
using,probed |
| R6068 |
T20674 |
T20675 |
det |
the,combination |
| R6069 |
T20675 |
T20673 |
dobj |
combination,using |
| R6070 |
T20676 |
T20675 |
compound |
antibody,combination |
| R6071 |
T20677 |
T20675 |
acl |
used,combination |
| R6072 |
T20678 |
T20677 |
prep |
for,used |
| R6073 |
T20679 |
T20680 |
compound |
kidney,immunohistochemistry |
| R6074 |
T20680 |
T20678 |
pobj |
immunohistochemistry,for |
| R6075 |
T20681 |
T20662 |
prep |
in,probed |
| R6076 |
T20682 |
T20681 |
pobj |
order,in |
| R6077 |
T20683 |
T20684 |
aux |
to,detect |
| R6078 |
T20684 |
T20682 |
acl |
detect,order |
| R6079 |
T20685 |
T20686 |
det |
the,AQP2 |
| R6080 |
T20686 |
T20684 |
dobj |
AQP2,detect |
| R6081 |
T20687 |
T20684 |
prep |
at,detect |
| R6082 |
T20688 |
T20689 |
nummod |
594,nm |
| R6083 |
T20689 |
T20687 |
pobj |
nm,at |
| R6084 |
T20690 |
T20684 |
punct |
", ",detect |
| R6085 |
T20691 |
T20692 |
aux |
to,distinguish |
| R6086 |
T20692 |
T20684 |
advcl |
distinguish,detect |
| R6087 |
T20693 |
T20692 |
prep |
between,distinguish |
| R6088 |
T20694 |
T20695 |
det |
the,proteins |
| R6089 |
T20695 |
T20693 |
pobj |
proteins,between |
| R6090 |
T20696 |
T20695 |
compound |
GFP,proteins |
| R6091 |
T20697 |
T20695 |
compound |
fusion,proteins |
| R6092 |
T20698 |
T20662 |
punct |
.,probed |
