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PMC:1189073 / 35783-36148 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T17909 0-6 NN denotes Kidney
T17910 7-16 NNS denotes membranes
T17912 17-18 -LRB- denotes (
T17914 18-20 CD denotes 60
T17913 21-23 NN denotes μg
T17915 23-24 -RRB- denotes )
T17916 25-29 VBD denotes were
T17911 30-39 VBN denotes incubated
T17917 40-42 IN denotes in
T17918 43-45 CD denotes 50
T17919 46-48 NN denotes mM
T17921 49-55 NN denotes sodium
T17920 56-65 NN denotes phosphate
T17922 66-67 -LRB- denotes (
T17923 67-69 NN denotes pH
T17924 70-73 CD denotes 5.5
T17925 73-74 -RRB- denotes )
T17926 74-76 , denotes ,
T17927 76-79 CD denotes 0.1
T17928 79-80 NN denotes %
T17929 81-84 NN denotes SDS
T17930 84-86 , denotes ,
T17931 86-89 CC denotes and
T17932 90-92 CD denotes 50
T17933 93-95 NN denotes mM
T17935 96-97 NN denotes β
T17936 97-98 HYPH denotes -
T17934 98-113 NN denotes mercaptoethanol
T17937 113-115 , denotes ,
T17938 115-121 VBN denotes heated
T17939 122-124 IN denotes to
T17940 125-128 CD denotes 100
T17941 129-131 NN denotes °C
T17942 132-135 IN denotes for
T17943 136-137 CD denotes 5
T17944 138-141 NN denotes min
T17945 141-143 , denotes ,
T17946 143-147 RB denotes then
T17947 148-154 VBN denotes cooled
T17948 154-155 . denotes .
T17949 155-243 sentence denotes Endoglycosidase H (0.01 units; Sigma-Aldrich) was added and incubated at 37 °C for 2 h.
T17950 156-171 NN denotes Endoglycosidase
T17951 172-173 NN denotes H
T17953 174-175 -LRB- denotes (
T17955 175-179 CD denotes 0.01
T17956 180-185 NNS denotes units
T17957 185-186 : denotes ;
T17958 187-192 NNP denotes Sigma
T17959 192-193 HYPH denotes -
T17954 193-200 NNP denotes Aldrich
T17960 200-201 -RRB- denotes )
T17961 202-205 VBD denotes was
T17952 206-211 VBN denotes added
T17962 212-215 CC denotes and
T17963 216-225 VBN denotes incubated
T17964 226-228 IN denotes at
T17965 229-231 CD denotes 37
T17966 232-234 NN denotes °C
T17967 235-238 IN denotes for
T17968 239-240 CD denotes 2
T17969 241-242 NN denotes h
T17970 242-243 . denotes .
T17971 243-310 sentence denotes The reaction was stopped by boiling the samples in Laemmli buffer.
T17972 244-247 DT denotes The
T17973 248-256 NN denotes reaction
T17975 257-260 VBD denotes was
T17974 261-268 VBN denotes stopped
T17976 269-271 IN denotes by
T17977 272-279 VBG denotes boiling
T17978 280-283 DT denotes the
T17979 284-291 NNS denotes samples
T17980 292-294 IN denotes in
T17981 295-302 NNP denotes Laemmli
T17982 303-309 NN denotes buffer
T17983 309-310 . denotes .
T17984 310-365 sentence denotes Total reactants were immunoblotted as described above.
T17985 311-316 JJ denotes Total
T17986 317-326 NNS denotes reactants
T17988 327-331 VBD denotes were
T17987 332-345 VBN denotes immunoblotted
T17989 346-348 IN denotes as
T17990 349-358 VBN denotes described
T17991 359-364 RB denotes above
T17992 364-365 . denotes .
R5215 T17909 T17910 compound Kidney,membranes
R5216 T17910 T17911 nsubjpass membranes,incubated
R5217 T17912 T17913 punct (,μg
R5218 T17913 T17910 parataxis μg,membranes
R5219 T17914 T17913 nummod 60,μg
R5220 T17915 T17913 punct ),μg
R5221 T17916 T17911 auxpass were,incubated
R5222 T17917 T17911 prep in,incubated
R5223 T17918 T17919 nummod 50,mM
R5224 T17919 T17920 compound mM,phosphate
R5225 T17920 T17917 pobj phosphate,in
R5226 T17921 T17920 compound sodium,phosphate
R5227 T17922 T17920 punct (,phosphate
R5228 T17923 T17920 npadvmod pH,phosphate
R5229 T17924 T17923 nummod 5.5,pH
R5230 T17925 T17920 punct ),phosphate
R5231 T17926 T17920 punct ", ",phosphate
R5232 T17927 T17928 nummod 0.1,%
R5233 T17928 T17929 compound %,SDS
R5234 T17929 T17920 appos SDS,phosphate
R5235 T17930 T17929 punct ", ",SDS
R5236 T17931 T17929 cc and,SDS
R5237 T17932 T17933 nummod 50,mM
R5238 T17933 T17934 compound mM,mercaptoethanol
R5239 T17934 T17929 conj mercaptoethanol,SDS
R5240 T17935 T17934 compound β,mercaptoethanol
R5241 T17936 T17934 punct -,mercaptoethanol
R5242 T17937 T17911 punct ", ",incubated
R5243 T17938 T17911 dep heated,incubated
R5244 T17939 T17938 prep to,heated
R5245 T17940 T17941 nummod 100,°C
R5246 T17941 T17939 pobj °C,to
R5247 T17942 T17938 prep for,heated
R5248 T17943 T17944 nummod 5,min
R5249 T17944 T17942 pobj min,for
R5250 T17945 T17911 punct ", ",incubated
R5251 T17946 T17947 advmod then,cooled
R5252 T17947 T17911 dep cooled,incubated
R5253 T17948 T17911 punct .,incubated
R5254 T17950 T17951 compound Endoglycosidase,H
R5255 T17951 T17952 nsubjpass H,added
R5256 T17953 T17954 punct (,Aldrich
R5257 T17954 T17951 parataxis Aldrich,H
R5258 T17955 T17956 nummod 0.01,units
R5259 T17956 T17954 dep units,Aldrich
R5260 T17957 T17954 punct ;,Aldrich
R5261 T17958 T17954 compound Sigma,Aldrich
R5262 T17959 T17954 punct -,Aldrich
R5263 T17960 T17954 punct ),Aldrich
R5264 T17961 T17952 auxpass was,added
R5265 T17962 T17952 cc and,added
R5266 T17963 T17952 conj incubated,added
R5267 T17964 T17963 prep at,incubated
R5268 T17965 T17966 nummod 37,°C
R5269 T17966 T17964 pobj °C,at
R5270 T17967 T17963 prep for,incubated
R5271 T17968 T17969 nummod 2,h
R5272 T17969 T17967 pobj h,for
R5273 T17970 T17952 punct .,added
R5274 T17972 T17973 det The,reaction
R5275 T17973 T17974 nsubjpass reaction,stopped
R5276 T17975 T17974 auxpass was,stopped
R5277 T17976 T17974 prep by,stopped
R5278 T17977 T17976 pcomp boiling,by
R5279 T17978 T17979 det the,samples
R5280 T17979 T17977 dobj samples,boiling
R5281 T17980 T17977 prep in,boiling
R5282 T17981 T17982 compound Laemmli,buffer
R5283 T17982 T17980 pobj buffer,in
R5284 T17983 T17974 punct .,stopped
R5285 T17985 T17986 amod Total,reactants
R5286 T17986 T17987 nsubjpass reactants,immunoblotted
R5287 T17988 T17987 auxpass were,immunoblotted
R5288 T17989 T17990 mark as,described
R5289 T17990 T17987 advcl described,immunoblotted
R5290 T17991 T17990 advmod above,described
R5291 T17992 T17987 punct .,immunoblotted