PMC:1189073 / 35152-35754 4 Projects
Annnotations
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T17617 | 0-6 | NN | denotes | Kidney |
| T17618 | 7-15 | NN | denotes | membrane |
| T17619 | 16-25 | NNS | denotes | fractions |
| T17621 | 26-27 | -LRB- | denotes | ( |
| T17623 | 27-29 | CD | denotes | 60 |
| T17622 | 30-32 | NN | denotes | μg |
| T17624 | 32-33 | -RRB- | denotes | ) |
| T17625 | 34-38 | VBD | denotes | were |
| T17620 | 39-47 | VBN | denotes | resolved |
| T17626 | 48-50 | IN | denotes | on |
| T17627 | 51-52 | DT | denotes | a |
| T17629 | 53-55 | CD | denotes | 12 |
| T17630 | 55-56 | NN | denotes | % |
| T17631 | 57-60 | NN | denotes | SDS |
| T17633 | 60-61 | HYPH | denotes | - |
| T17632 | 61-75 | NN | denotes | polyacrylamide |
| T17628 | 76-79 | NN | denotes | gel |
| T17634 | 80-83 | CC | denotes | and |
| T17635 | 84-95 | VBN | denotes | transferred |
| T17636 | 96-98 | IN | denotes | to |
| T17637 | 99-100 | DT | denotes | a |
| T17639 | 101-115 | NN | denotes | nitrocellulose |
| T17638 | 116-124 | NN | denotes | membrane |
| T17640 | 124-125 | . | denotes | . |
| T17641 | 125-371 | sentence | denotes | Membranes were blocked in 5% nonfat milk in Tris-buffered saline with 0.05% Tween 20 (TBST), followed by an overnight incubation (at 4 °C) with AQP2 polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, California, United States; #sc-9882). |
| T17642 | 126-135 | NNS | denotes | Membranes |
| T17644 | 136-140 | VBD | denotes | were |
| T17643 | 141-148 | VBN | denotes | blocked |
| T17645 | 149-151 | IN | denotes | in |
| T17646 | 152-153 | CD | denotes | 5 |
| T17647 | 153-154 | NN | denotes | % |
| T17649 | 155-161 | JJ | denotes | nonfat |
| T17648 | 162-166 | NN | denotes | milk |
| T17650 | 167-169 | IN | denotes | in |
| T17651 | 170-174 | NN | denotes | Tris |
| T17653 | 174-175 | HYPH | denotes | - |
| T17652 | 175-183 | VBN | denotes | buffered |
| T17654 | 184-190 | NN | denotes | saline |
| T17655 | 191-195 | IN | denotes | with |
| T17656 | 196-200 | CD | denotes | 0.05 |
| T17657 | 200-201 | NN | denotes | % |
| T17658 | 202-207 | NN | denotes | Tween |
| T17659 | 208-210 | CD | denotes | 20 |
| T17660 | 211-212 | -LRB- | denotes | ( |
| T17661 | 212-216 | NN | denotes | TBST |
| T17662 | 216-217 | -RRB- | denotes | ) |
| T17663 | 217-219 | , | denotes | , |
| T17664 | 219-227 | VBN | denotes | followed |
| T17665 | 228-230 | IN | denotes | by |
| T17666 | 231-233 | DT | denotes | an |
| T17668 | 234-243 | JJ | denotes | overnight |
| T17667 | 244-254 | NN | denotes | incubation |
| T17669 | 255-256 | -LRB- | denotes | ( |
| T17670 | 256-258 | IN | denotes | at |
| T17671 | 259-260 | CD | denotes | 4 |
| T17672 | 261-263 | NN | denotes | °C |
| T17673 | 263-264 | -RRB- | denotes | ) |
| T17674 | 265-269 | IN | denotes | with |
| T17675 | 270-274 | NN | denotes | AQP2 |
| T17677 | 275-285 | JJ | denotes | polyclonal |
| T17676 | 286-294 | NN | denotes | antibody |
| T17678 | 295-296 | -LRB- | denotes | ( |
| T17680 | 296-301 | NNP | denotes | Santa |
| T17681 | 302-306 | NNP | denotes | Cruz |
| T17682 | 307-320 | NNP | denotes | Biotechnology |
| T17683 | 320-322 | , | denotes | , |
| T17684 | 322-327 | NNP | denotes | Santa |
| T17685 | 328-332 | NNP | denotes | Cruz |
| T17686 | 332-334 | , | denotes | , |
| T17687 | 334-344 | NNP | denotes | California |
| T17688 | 344-346 | , | denotes | , |
| T17689 | 346-352 | NNP | denotes | United |
| T17690 | 353-359 | NNP | denotes | States |
| T17691 | 359-360 | : | denotes | ; |
| T17692 | 361-362 | SYM | denotes | # |
| T17679 | 362-364 | NN | denotes | sc |
| T17693 | 364-365 | HYPH | denotes | - |
| T17694 | 365-369 | CD | denotes | 9882 |
| T17695 | 369-370 | -RRB- | denotes | ) |
| T17696 | 370-371 | . | denotes | . |
| T17697 | 371-463 | sentence | denotes | Membranes were washed in TBST then incubated with HRP-conjugated donkey anti-goat antibody. |
| T17698 | 372-381 | NNS | denotes | Membranes |
| T17700 | 382-386 | VBD | denotes | were |
| T17699 | 387-393 | VBN | denotes | washed |
| T17701 | 394-396 | IN | denotes | in |
| T17702 | 397-401 | NN | denotes | TBST |
| T17703 | 402-406 | RB | denotes | then |
| T17704 | 407-416 | VBN | denotes | incubated |
| T17705 | 417-421 | IN | denotes | with |
| T17706 | 422-425 | NN | denotes | HRP |
| T17708 | 425-426 | HYPH | denotes | - |
| T17707 | 426-436 | VBN | denotes | conjugated |
| T17710 | 437-443 | NN | denotes | donkey |
| T17711 | 444-453 | JJ | denotes | anti-goat |
| T17709 | 454-462 | NN | denotes | antibody |
| T17712 | 462-463 | . | denotes | . |
| T17713 | 463-602 | sentence | denotes | Membranes were washed further in TBST and bands were visualized using ECL reagent (Amersham Biosciences, Little Chalfont, United Kingdom). |
| T17714 | 464-473 | NNS | denotes | Membranes |
| T17716 | 474-478 | VBD | denotes | were |
| T17715 | 479-485 | VBN | denotes | washed |
| T17717 | 486-493 | RB | denotes | further |
| T17718 | 494-496 | IN | denotes | in |
| T17719 | 497-501 | NN | denotes | TBST |
| T17720 | 502-505 | CC | denotes | and |
| T17721 | 506-511 | NNS | denotes | bands |
| T17723 | 512-516 | VBD | denotes | were |
| T17722 | 517-527 | VBN | denotes | visualized |
| T17724 | 528-533 | VBG | denotes | using |
| T17725 | 534-537 | NN | denotes | ECL |
| T17726 | 538-545 | NN | denotes | reagent |
| T17727 | 546-547 | -LRB- | denotes | ( |
| T17729 | 547-555 | NNP | denotes | Amersham |
| T17728 | 556-567 | NNP | denotes | Biosciences |
| T17730 | 567-569 | , | denotes | , |
| T17731 | 569-575 | NNP | denotes | Little |
| T17732 | 576-584 | NNP | denotes | Chalfont |
| T17733 | 584-586 | , | denotes | , |
| T17734 | 586-592 | NNP | denotes | United |
| T17735 | 593-600 | NNP | denotes | Kingdom |
| T17736 | 600-601 | -RRB- | denotes | ) |
| T17737 | 601-602 | . | denotes | . |
| R5099 | T17617 | T17618 | compound | Kidney,membrane |
| R5100 | T17618 | T17619 | compound | membrane,fractions |
| R5101 | T17619 | T17620 | nsubjpass | fractions,resolved |
| R5102 | T17621 | T17622 | punct | (,μg |
| R5103 | T17622 | T17619 | parataxis | μg,fractions |
| R5104 | T17623 | T17622 | nummod | 60,μg |
| R5105 | T17624 | T17622 | punct | ),μg |
| R5106 | T17625 | T17620 | auxpass | were,resolved |
| R5107 | T17626 | T17620 | prep | on,resolved |
| R5108 | T17627 | T17628 | det | a,gel |
| R5109 | T17628 | T17626 | pobj | gel,on |
| R5110 | T17629 | T17630 | nummod | 12,% |
| R5111 | T17630 | T17628 | compound | %,gel |
| R5112 | T17631 | T17632 | compound | SDS,polyacrylamide |
| R5113 | T17632 | T17628 | compound | polyacrylamide,gel |
| R5114 | T17633 | T17632 | punct | -,polyacrylamide |
| R5115 | T17634 | T17620 | cc | and,resolved |
| R5116 | T17635 | T17620 | conj | transferred,resolved |
| R5117 | T17636 | T17635 | prep | to,transferred |
| R5118 | T17637 | T17638 | det | a,membrane |
| R5119 | T17638 | T17636 | pobj | membrane,to |
| R5120 | T17639 | T17638 | compound | nitrocellulose,membrane |
| R5121 | T17640 | T17620 | punct | .,resolved |
| R5122 | T17642 | T17643 | nsubjpass | Membranes,blocked |
| R5123 | T17644 | T17643 | auxpass | were,blocked |
| R5124 | T17645 | T17643 | prep | in,blocked |
| R5125 | T17646 | T17647 | nummod | 5,% |
| R5126 | T17647 | T17648 | nmod | %,milk |
| R5127 | T17648 | T17645 | pobj | milk,in |
| R5128 | T17649 | T17648 | amod | nonfat,milk |
| R5129 | T17650 | T17648 | prep | in,milk |
| R5130 | T17651 | T17652 | npadvmod | Tris,buffered |
| R5131 | T17652 | T17654 | amod | buffered,saline |
| R5132 | T17653 | T17652 | punct | -,buffered |
| R5133 | T17654 | T17650 | pobj | saline,in |
| R5134 | T17655 | T17654 | prep | with,saline |
| R5135 | T17656 | T17657 | nummod | 0.05,% |
| R5136 | T17657 | T17658 | compound | %,Tween |
| R5137 | T17658 | T17655 | pobj | Tween,with |
| R5138 | T17659 | T17658 | nummod | 20,Tween |
| R5139 | T17660 | T17661 | punct | (,TBST |
| R5140 | T17661 | T17658 | parataxis | TBST,Tween |
| R5141 | T17662 | T17661 | punct | ),TBST |
| R5142 | T17663 | T17643 | punct | ", ",blocked |
| R5143 | T17664 | T17643 | advcl | followed,blocked |
| R5144 | T17665 | T17664 | agent | by,followed |
| R5145 | T17666 | T17667 | det | an,incubation |
| R5146 | T17667 | T17665 | pobj | incubation,by |
| R5147 | T17668 | T17667 | amod | overnight,incubation |
| R5148 | T17669 | T17667 | punct | (,incubation |
| R5149 | T17670 | T17667 | prep | at,incubation |
| R5150 | T17671 | T17672 | nummod | 4,°C |
| R5151 | T17672 | T17670 | pobj | °C,at |
| R5152 | T17673 | T17667 | punct | ),incubation |
| R5153 | T17674 | T17667 | prep | with,incubation |
| R5154 | T17675 | T17676 | nmod | AQP2,antibody |
| R5155 | T17676 | T17674 | pobj | antibody,with |
| R5156 | T17677 | T17676 | amod | polyclonal,antibody |
| R5157 | T17678 | T17679 | punct | (,sc |
| R5158 | T17679 | T17676 | parataxis | sc,antibody |
| R5159 | T17680 | T17681 | compound | Santa,Cruz |
| R5160 | T17681 | T17682 | compound | Cruz,Biotechnology |
| R5161 | T17682 | T17679 | dep | Biotechnology,sc |
| R5162 | T17683 | T17682 | punct | ", ",Biotechnology |
| R5163 | T17684 | T17685 | compound | Santa,Cruz |
| R5164 | T17685 | T17682 | npadvmod | Cruz,Biotechnology |
| R5165 | T17686 | T17682 | punct | ", ",Biotechnology |
| R5166 | T17687 | T17682 | npadvmod | California,Biotechnology |
| R5167 | T17688 | T17682 | punct | ", ",Biotechnology |
| R5168 | T17689 | T17690 | compound | United,States |
| R5169 | T17690 | T17682 | npadvmod | States,Biotechnology |
| R5170 | T17691 | T17679 | punct | ;,sc |
| R5171 | T17692 | T17679 | punct | #,sc |
| R5172 | T17693 | T17679 | punct | -,sc |
| R5173 | T17694 | T17679 | nummod | 9882,sc |
| R5174 | T17695 | T17679 | punct | ),sc |
| R5175 | T17696 | T17643 | punct | .,blocked |
| R5176 | T17698 | T17699 | nsubjpass | Membranes,washed |
| R5177 | T17700 | T17699 | auxpass | were,washed |
| R5178 | T17701 | T17699 | prep | in,washed |
| R5179 | T17702 | T17701 | pobj | TBST,in |
| R5180 | T17703 | T17704 | advmod | then,incubated |
| R5181 | T17704 | T17699 | dep | incubated,washed |
| R5182 | T17705 | T17704 | prep | with,incubated |
| R5183 | T17706 | T17707 | npadvmod | HRP,conjugated |
| R5184 | T17707 | T17709 | amod | conjugated,antibody |
| R5185 | T17708 | T17707 | punct | -,conjugated |
| R5186 | T17709 | T17705 | pobj | antibody,with |
| R5187 | T17710 | T17709 | nmod | donkey,antibody |
| R5188 | T17711 | T17709 | amod | anti-goat,antibody |
| R5189 | T17712 | T17699 | punct | .,washed |
| R5190 | T17714 | T17715 | nsubjpass | Membranes,washed |
| R5191 | T17716 | T17715 | auxpass | were,washed |
| R5192 | T17717 | T17715 | advmod | further,washed |
| R5193 | T17718 | T17715 | prep | in,washed |
| R5194 | T17719 | T17718 | pobj | TBST,in |
| R5195 | T17720 | T17715 | cc | and,washed |
| R5196 | T17721 | T17722 | nsubjpass | bands,visualized |
| R5197 | T17722 | T17715 | conj | visualized,washed |
| R5198 | T17723 | T17722 | auxpass | were,visualized |
| R5199 | T17724 | T17722 | advcl | using,visualized |
| R5200 | T17725 | T17726 | compound | ECL,reagent |
| R5201 | T17726 | T17724 | dobj | reagent,using |
| R5202 | T17727 | T17728 | punct | (,Biosciences |
| R5203 | T17728 | T17726 | parataxis | Biosciences,reagent |
| R5204 | T17729 | T17728 | compound | Amersham,Biosciences |
| R5205 | T17730 | T17728 | punct | ", ",Biosciences |
| R5206 | T17731 | T17732 | compound | Little,Chalfont |
| R5207 | T17732 | T17728 | npadvmod | Chalfont,Biosciences |
| R5208 | T17733 | T17728 | punct | ", ",Biosciences |
| R5209 | T17734 | T17735 | compound | United,Kingdom |
| R5210 | T17735 | T17728 | npadvmod | Kingdom,Biosciences |
| R5211 | T17736 | T17728 | punct | ),Biosciences |
| R5212 | T17737 | T17722 | punct | .,visualized |