| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T17614 |
0-14 |
NN |
denotes |
Immunoblotting |
| T17615 |
14-15 |
. |
denotes |
. |
| T17616 |
15-141 |
sentence |
denotes |
Kidney membrane fractions (60 μg) were resolved on a 12% SDS-polyacrylamide gel and transferred to a nitrocellulose membrane. |
| T17617 |
16-22 |
NN |
denotes |
Kidney |
| T17618 |
23-31 |
NN |
denotes |
membrane |
| T17619 |
32-41 |
NNS |
denotes |
fractions |
| T17621 |
42-43 |
-LRB- |
denotes |
( |
| T17623 |
43-45 |
CD |
denotes |
60 |
| T17622 |
46-48 |
NN |
denotes |
μg |
| T17624 |
48-49 |
-RRB- |
denotes |
) |
| T17625 |
50-54 |
VBD |
denotes |
were |
| T17620 |
55-63 |
VBN |
denotes |
resolved |
| T17626 |
64-66 |
IN |
denotes |
on |
| T17627 |
67-68 |
DT |
denotes |
a |
| T17629 |
69-71 |
CD |
denotes |
12 |
| T17630 |
71-72 |
NN |
denotes |
% |
| T17631 |
73-76 |
NN |
denotes |
SDS |
| T17633 |
76-77 |
HYPH |
denotes |
- |
| T17632 |
77-91 |
NN |
denotes |
polyacrylamide |
| T17628 |
92-95 |
NN |
denotes |
gel |
| T17634 |
96-99 |
CC |
denotes |
and |
| T17635 |
100-111 |
VBN |
denotes |
transferred |
| T17636 |
112-114 |
IN |
denotes |
to |
| T17637 |
115-116 |
DT |
denotes |
a |
| T17639 |
117-131 |
NN |
denotes |
nitrocellulose |
| T17638 |
132-140 |
NN |
denotes |
membrane |
| T17640 |
140-141 |
. |
denotes |
. |
| T17641 |
141-387 |
sentence |
denotes |
Membranes were blocked in 5% nonfat milk in Tris-buffered saline with 0.05% Tween 20 (TBST), followed by an overnight incubation (at 4 °C) with AQP2 polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, California, United States; #sc-9882). |
| T17642 |
142-151 |
NNS |
denotes |
Membranes |
| T17644 |
152-156 |
VBD |
denotes |
were |
| T17643 |
157-164 |
VBN |
denotes |
blocked |
| T17645 |
165-167 |
IN |
denotes |
in |
| T17646 |
168-169 |
CD |
denotes |
5 |
| T17647 |
169-170 |
NN |
denotes |
% |
| T17649 |
171-177 |
JJ |
denotes |
nonfat |
| T17648 |
178-182 |
NN |
denotes |
milk |
| T17650 |
183-185 |
IN |
denotes |
in |
| T17651 |
186-190 |
NN |
denotes |
Tris |
| T17653 |
190-191 |
HYPH |
denotes |
- |
| T17652 |
191-199 |
VBN |
denotes |
buffered |
| T17654 |
200-206 |
NN |
denotes |
saline |
| T17655 |
207-211 |
IN |
denotes |
with |
| T17656 |
212-216 |
CD |
denotes |
0.05 |
| T17657 |
216-217 |
NN |
denotes |
% |
| T17658 |
218-223 |
NN |
denotes |
Tween |
| T17659 |
224-226 |
CD |
denotes |
20 |
| T17660 |
227-228 |
-LRB- |
denotes |
( |
| T17661 |
228-232 |
NN |
denotes |
TBST |
| T17662 |
232-233 |
-RRB- |
denotes |
) |
| T17663 |
233-235 |
, |
denotes |
, |
| T17664 |
235-243 |
VBN |
denotes |
followed |
| T17665 |
244-246 |
IN |
denotes |
by |
| T17666 |
247-249 |
DT |
denotes |
an |
| T17668 |
250-259 |
JJ |
denotes |
overnight |
| T17667 |
260-270 |
NN |
denotes |
incubation |
| T17669 |
271-272 |
-LRB- |
denotes |
( |
| T17670 |
272-274 |
IN |
denotes |
at |
| T17671 |
275-276 |
CD |
denotes |
4 |
| T17672 |
277-279 |
NN |
denotes |
°C |
| T17673 |
279-280 |
-RRB- |
denotes |
) |
| T17674 |
281-285 |
IN |
denotes |
with |
| T17675 |
286-290 |
NN |
denotes |
AQP2 |
| T17677 |
291-301 |
JJ |
denotes |
polyclonal |
| T17676 |
302-310 |
NN |
denotes |
antibody |
| T17678 |
311-312 |
-LRB- |
denotes |
( |
| T17680 |
312-317 |
NNP |
denotes |
Santa |
| T17681 |
318-322 |
NNP |
denotes |
Cruz |
| T17682 |
323-336 |
NNP |
denotes |
Biotechnology |
| T17683 |
336-338 |
, |
denotes |
, |
| T17684 |
338-343 |
NNP |
denotes |
Santa |
| T17685 |
344-348 |
NNP |
denotes |
Cruz |
| T17686 |
348-350 |
, |
denotes |
, |
| T17687 |
350-360 |
NNP |
denotes |
California |
| T17688 |
360-362 |
, |
denotes |
, |
| T17689 |
362-368 |
NNP |
denotes |
United |
| T17690 |
369-375 |
NNP |
denotes |
States |
| T17691 |
375-376 |
: |
denotes |
; |
| T17692 |
377-378 |
SYM |
denotes |
# |
| T17679 |
378-380 |
NN |
denotes |
sc |
| T17693 |
380-381 |
HYPH |
denotes |
- |
| T17694 |
381-385 |
CD |
denotes |
9882 |
| T17695 |
385-386 |
-RRB- |
denotes |
) |
| T17696 |
386-387 |
. |
denotes |
. |
| T17697 |
387-479 |
sentence |
denotes |
Membranes were washed in TBST then incubated with HRP-conjugated donkey anti-goat antibody. |
| T17698 |
388-397 |
NNS |
denotes |
Membranes |
| T17700 |
398-402 |
VBD |
denotes |
were |
| T17699 |
403-409 |
VBN |
denotes |
washed |
| T17701 |
410-412 |
IN |
denotes |
in |
| T17702 |
413-417 |
NN |
denotes |
TBST |
| T17703 |
418-422 |
RB |
denotes |
then |
| T17704 |
423-432 |
VBN |
denotes |
incubated |
| T17705 |
433-437 |
IN |
denotes |
with |
| T17706 |
438-441 |
NN |
denotes |
HRP |
| T17708 |
441-442 |
HYPH |
denotes |
- |
| T17707 |
442-452 |
VBN |
denotes |
conjugated |
| T17710 |
453-459 |
NN |
denotes |
donkey |
| T17711 |
460-469 |
JJ |
denotes |
anti-goat |
| T17709 |
470-478 |
NN |
denotes |
antibody |
| T17712 |
478-479 |
. |
denotes |
. |
| T17713 |
479-618 |
sentence |
denotes |
Membranes were washed further in TBST and bands were visualized using ECL reagent (Amersham Biosciences, Little Chalfont, United Kingdom). |
| T17714 |
480-489 |
NNS |
denotes |
Membranes |
| T17716 |
490-494 |
VBD |
denotes |
were |
| T17715 |
495-501 |
VBN |
denotes |
washed |
| T17717 |
502-509 |
RB |
denotes |
further |
| T17718 |
510-512 |
IN |
denotes |
in |
| T17719 |
513-517 |
NN |
denotes |
TBST |
| T17720 |
518-521 |
CC |
denotes |
and |
| T17721 |
522-527 |
NNS |
denotes |
bands |
| T17723 |
528-532 |
VBD |
denotes |
were |
| T17722 |
533-543 |
VBN |
denotes |
visualized |
| T17724 |
544-549 |
VBG |
denotes |
using |
| T17725 |
550-553 |
NN |
denotes |
ECL |
| T17726 |
554-561 |
NN |
denotes |
reagent |
| T17727 |
562-563 |
-LRB- |
denotes |
( |
| T17729 |
563-571 |
NNP |
denotes |
Amersham |
| T17728 |
572-583 |
NNP |
denotes |
Biosciences |
| T17730 |
583-585 |
, |
denotes |
, |
| T17731 |
585-591 |
NNP |
denotes |
Little |
| T17732 |
592-600 |
NNP |
denotes |
Chalfont |
| T17733 |
600-602 |
, |
denotes |
, |
| T17734 |
602-608 |
NNP |
denotes |
United |
| T17735 |
609-616 |
NNP |
denotes |
Kingdom |
| T17736 |
616-617 |
-RRB- |
denotes |
) |
| T17737 |
617-618 |
. |
denotes |
. |
| R5098 |
T17615 |
T17614 |
punct |
.,Immunoblotting |
| R5099 |
T17617 |
T17618 |
compound |
Kidney,membrane |
| R5100 |
T17618 |
T17619 |
compound |
membrane,fractions |
| R5101 |
T17619 |
T17620 |
nsubjpass |
fractions,resolved |
| R5102 |
T17621 |
T17622 |
punct |
(,μg |
| R5103 |
T17622 |
T17619 |
parataxis |
μg,fractions |
| R5104 |
T17623 |
T17622 |
nummod |
60,μg |
| R5105 |
T17624 |
T17622 |
punct |
),μg |
| R5106 |
T17625 |
T17620 |
auxpass |
were,resolved |
| R5107 |
T17626 |
T17620 |
prep |
on,resolved |
| R5108 |
T17627 |
T17628 |
det |
a,gel |
| R5109 |
T17628 |
T17626 |
pobj |
gel,on |
| R5110 |
T17629 |
T17630 |
nummod |
12,% |
| R5111 |
T17630 |
T17628 |
compound |
%,gel |
| R5112 |
T17631 |
T17632 |
compound |
SDS,polyacrylamide |
| R5113 |
T17632 |
T17628 |
compound |
polyacrylamide,gel |
| R5114 |
T17633 |
T17632 |
punct |
-,polyacrylamide |
| R5115 |
T17634 |
T17620 |
cc |
and,resolved |
| R5116 |
T17635 |
T17620 |
conj |
transferred,resolved |
| R5117 |
T17636 |
T17635 |
prep |
to,transferred |
| R5118 |
T17637 |
T17638 |
det |
a,membrane |
| R5119 |
T17638 |
T17636 |
pobj |
membrane,to |
| R5120 |
T17639 |
T17638 |
compound |
nitrocellulose,membrane |
| R5121 |
T17640 |
T17620 |
punct |
.,resolved |
| R5122 |
T17642 |
T17643 |
nsubjpass |
Membranes,blocked |
| R5123 |
T17644 |
T17643 |
auxpass |
were,blocked |
| R5124 |
T17645 |
T17643 |
prep |
in,blocked |
| R5125 |
T17646 |
T17647 |
nummod |
5,% |
| R5126 |
T17647 |
T17648 |
nmod |
%,milk |
| R5127 |
T17648 |
T17645 |
pobj |
milk,in |
| R5128 |
T17649 |
T17648 |
amod |
nonfat,milk |
| R5129 |
T17650 |
T17648 |
prep |
in,milk |
| R5130 |
T17651 |
T17652 |
npadvmod |
Tris,buffered |
| R5131 |
T17652 |
T17654 |
amod |
buffered,saline |
| R5132 |
T17653 |
T17652 |
punct |
-,buffered |
| R5133 |
T17654 |
T17650 |
pobj |
saline,in |
| R5134 |
T17655 |
T17654 |
prep |
with,saline |
| R5135 |
T17656 |
T17657 |
nummod |
0.05,% |
| R5136 |
T17657 |
T17658 |
compound |
%,Tween |
| R5137 |
T17658 |
T17655 |
pobj |
Tween,with |
| R5138 |
T17659 |
T17658 |
nummod |
20,Tween |
| R5139 |
T17660 |
T17661 |
punct |
(,TBST |
| R5140 |
T17661 |
T17658 |
parataxis |
TBST,Tween |
| R5141 |
T17662 |
T17661 |
punct |
),TBST |
| R5142 |
T17663 |
T17643 |
punct |
", ",blocked |
| R5143 |
T17664 |
T17643 |
advcl |
followed,blocked |
| R5144 |
T17665 |
T17664 |
agent |
by,followed |
| R5145 |
T17666 |
T17667 |
det |
an,incubation |
| R5146 |
T17667 |
T17665 |
pobj |
incubation,by |
| R5147 |
T17668 |
T17667 |
amod |
overnight,incubation |
| R5148 |
T17669 |
T17667 |
punct |
(,incubation |
| R5149 |
T17670 |
T17667 |
prep |
at,incubation |
| R5150 |
T17671 |
T17672 |
nummod |
4,°C |
| R5151 |
T17672 |
T17670 |
pobj |
°C,at |
| R5152 |
T17673 |
T17667 |
punct |
),incubation |
| R5153 |
T17674 |
T17667 |
prep |
with,incubation |
| R5154 |
T17675 |
T17676 |
nmod |
AQP2,antibody |
| R5155 |
T17676 |
T17674 |
pobj |
antibody,with |
| R5156 |
T17677 |
T17676 |
amod |
polyclonal,antibody |
| R5157 |
T17678 |
T17679 |
punct |
(,sc |
| R5158 |
T17679 |
T17676 |
parataxis |
sc,antibody |
| R5159 |
T17680 |
T17681 |
compound |
Santa,Cruz |
| R5160 |
T17681 |
T17682 |
compound |
Cruz,Biotechnology |
| R5161 |
T17682 |
T17679 |
dep |
Biotechnology,sc |
| R5162 |
T17683 |
T17682 |
punct |
", ",Biotechnology |
| R5163 |
T17684 |
T17685 |
compound |
Santa,Cruz |
| R5164 |
T17685 |
T17682 |
npadvmod |
Cruz,Biotechnology |
| R5165 |
T17686 |
T17682 |
punct |
", ",Biotechnology |
| R5166 |
T17687 |
T17682 |
npadvmod |
California,Biotechnology |
| R5167 |
T17688 |
T17682 |
punct |
", ",Biotechnology |
| R5168 |
T17689 |
T17690 |
compound |
United,States |
| R5169 |
T17690 |
T17682 |
npadvmod |
States,Biotechnology |
| R5170 |
T17691 |
T17679 |
punct |
;,sc |
| R5171 |
T17692 |
T17679 |
punct |
#,sc |
| R5172 |
T17693 |
T17679 |
punct |
-,sc |
| R5173 |
T17694 |
T17679 |
nummod |
9882,sc |
| R5174 |
T17695 |
T17679 |
punct |
),sc |
| R5175 |
T17696 |
T17643 |
punct |
.,blocked |
| R5176 |
T17698 |
T17699 |
nsubjpass |
Membranes,washed |
| R5177 |
T17700 |
T17699 |
auxpass |
were,washed |
| R5178 |
T17701 |
T17699 |
prep |
in,washed |
| R5179 |
T17702 |
T17701 |
pobj |
TBST,in |
| R5180 |
T17703 |
T17704 |
advmod |
then,incubated |
| R5181 |
T17704 |
T17699 |
dep |
incubated,washed |
| R5182 |
T17705 |
T17704 |
prep |
with,incubated |
| R5183 |
T17706 |
T17707 |
npadvmod |
HRP,conjugated |
| R5184 |
T17707 |
T17709 |
amod |
conjugated,antibody |
| R5185 |
T17708 |
T17707 |
punct |
-,conjugated |
| R5186 |
T17709 |
T17705 |
pobj |
antibody,with |
| R5187 |
T17710 |
T17709 |
nmod |
donkey,antibody |
| R5188 |
T17711 |
T17709 |
amod |
anti-goat,antibody |
| R5189 |
T17712 |
T17699 |
punct |
.,washed |
| R5190 |
T17714 |
T17715 |
nsubjpass |
Membranes,washed |
| R5191 |
T17716 |
T17715 |
auxpass |
were,washed |
| R5192 |
T17717 |
T17715 |
advmod |
further,washed |
| R5193 |
T17718 |
T17715 |
prep |
in,washed |
| R5194 |
T17719 |
T17718 |
pobj |
TBST,in |
| R5195 |
T17720 |
T17715 |
cc |
and,washed |
| R5196 |
T17721 |
T17722 |
nsubjpass |
bands,visualized |
| R5197 |
T17722 |
T17715 |
conj |
visualized,washed |
| R5198 |
T17723 |
T17722 |
auxpass |
were,visualized |
| R5199 |
T17724 |
T17722 |
advcl |
using,visualized |
| R5200 |
T17725 |
T17726 |
compound |
ECL,reagent |
| R5201 |
T17726 |
T17724 |
dobj |
reagent,using |
| R5202 |
T17727 |
T17728 |
punct |
(,Biosciences |
| R5203 |
T17728 |
T17726 |
parataxis |
Biosciences,reagent |
| R5204 |
T17729 |
T17728 |
compound |
Amersham,Biosciences |
| R5205 |
T17730 |
T17728 |
punct |
", ",Biosciences |
| R5206 |
T17731 |
T17732 |
compound |
Little,Chalfont |
| R5207 |
T17732 |
T17728 |
npadvmod |
Chalfont,Biosciences |
| R5208 |
T17733 |
T17728 |
punct |
", ",Biosciences |
| R5209 |
T17734 |
T17735 |
compound |
United,Kingdom |
| R5210 |
T17735 |
T17728 |
npadvmod |
Kingdom,Biosciences |
| R5211 |
T17736 |
T17728 |
punct |
),Biosciences |
| R5212 |
T17737 |
T17722 |
punct |
.,visualized |
