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PMC:1189073 / 23586-25151 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T11145 0-2 IN denotes If
T11147 3-7 JJ denotes wild
T11149 7-8 HYPH denotes -
T11148 8-12 NN denotes type
T11151 13-16 CC denotes and
T11152 17-23 NN denotes mutant
T11150 24-32 NN denotes proteins
T11153 33-36 VBP denotes are
T11154 37-43 RB denotes indeed
T11146 44-55 VBG denotes interacting
T11156 56-58 IN denotes in
T11157 59-62 DT denotes the
T11158 63-67 NN denotes cell
T11159 67-69 , denotes ,
T11155 69-71 VBZ denotes is
T11160 72-76 DT denotes this
T11161 77-88 NN denotes interaction
T11162 89-99 JJ denotes sufficient
T11163 100-102 TO denotes to
T11164 103-109 VB denotes rescue
T11165 110-113 DT denotes the
T11166 114-126 NN denotes localization
T11167 127-129 IN denotes of
T11168 130-136 NN denotes mutant
T11169 137-144 NN denotes protein
T11170 144-145 . denotes ?
T11171 145-268 sentence denotes To answer this question, we used MDCK cells stably transfected with wild-type AQP2 expressing vector or with empty vector.
T11172 146-148 TO denotes To
T11173 149-155 VB denotes answer
T11175 156-160 DT denotes this
T11176 161-169 NN denotes question
T11177 169-171 , denotes ,
T11178 171-173 PRP denotes we
T11174 174-178 VBD denotes used
T11179 179-183 NN denotes MDCK
T11180 184-189 NNS denotes cells
T11181 190-196 RB denotes stably
T11182 197-208 VBN denotes transfected
T11183 209-213 IN denotes with
T11184 214-218 JJ denotes wild
T11186 218-219 HYPH denotes -
T11185 219-223 NN denotes type
T11187 224-228 NN denotes AQP2
T11188 229-239 VBG denotes expressing
T11189 240-246 NN denotes vector
T11190 247-249 CC denotes or
T11191 250-254 IN denotes with
T11192 255-260 JJ denotes empty
T11193 261-267 NN denotes vector
T11194 267-268 . denotes .
T11195 268-362 sentence denotes On top of these, we transiently transfected AQP2-GFP or AQP2-F204V-GFP expression constructs.
T11196 269-271 IN denotes On
T11198 272-275 NN denotes top
T11199 276-278 IN denotes of
T11200 279-284 DT denotes these
T11201 284-286 , denotes ,
T11202 286-288 PRP denotes we
T11203 289-300 RB denotes transiently
T11197 301-312 VBD denotes transfected
T11204 313-317 NN denotes AQP2
T11206 317-318 HYPH denotes -
T11205 318-321 NN denotes GFP
T11208 322-324 CC denotes or
T11209 325-329 NN denotes AQP2
T11211 329-330 HYPH denotes -
T11212 330-335 NN denotes F204V
T11213 335-336 HYPH denotes -
T11210 336-339 NN denotes GFP
T11214 340-350 NN denotes expression
T11207 351-361 NNS denotes constructs
T11215 361-362 . denotes .
T11216 362-578 sentence denotes AQP2-GFP localized to the apical surface upon forskolin stimulation whether it was transiently transfected into vector-only cells (Figure 5C, upper left images) or into wild-type AQP2 cells (Figure 5C, upper right).
T11217 363-367 NN denotes AQP2
T11219 367-368 HYPH denotes -
T11218 368-371 NN denotes GFP
T11220 372-381 VBD denotes localized
T11221 382-384 IN denotes to
T11222 385-388 DT denotes the
T11224 389-395 JJ denotes apical
T11223 396-403 NN denotes surface
T11225 404-408 IN denotes upon
T11226 409-418 NN denotes forskolin
T11227 419-430 NN denotes stimulation
T11228 431-438 IN denotes whether
T11230 439-441 PRP denotes it
T11231 442-445 VBD denotes was
T11232 446-457 RB denotes transiently
T11229 458-469 VBN denotes transfected
T11233 470-474 IN denotes into
T11234 475-481 NN denotes vector
T11236 481-482 HYPH denotes -
T11235 482-486 JJ denotes only
T11237 487-492 NNS denotes cells
T11238 493-494 -LRB- denotes (
T11240 494-500 NN denotes Figure
T11241 501-503 CD denotes 5C
T11242 503-505 , denotes ,
T11243 505-510 JJ denotes upper
T11244 511-515 JJ denotes left
T11239 516-522 NNS denotes images
T11245 522-523 -RRB- denotes )
T11246 524-526 CC denotes or
T11247 527-531 IN denotes into
T11248 532-536 JJ denotes wild
T11250 536-537 HYPH denotes -
T11249 537-541 NN denotes type
T11252 542-546 NN denotes AQP2
T11251 547-552 NNS denotes cells
T11253 553-554 -LRB- denotes (
T11254 554-560 NN denotes Figure
T11255 561-563 CD denotes 5C
T11256 563-565 , denotes ,
T11257 565-570 JJ denotes upper
T11258 571-576 JJ denotes right
T11259 576-577 -RRB- denotes )
T11260 577-578 . denotes .
T11261 578-734 sentence denotes AQP2-F204V-GFP, when expressed by transient transfection into vector only cells, showed a diffuse cytoplasmic distribution pattern (Figure 5C, lower left).
T11262 579-583 NN denotes AQP2
T11264 583-584 HYPH denotes -
T11265 584-589 NN denotes F204V
T11266 589-590 HYPH denotes -
T11263 590-593 NN denotes GFP
T11268 593-595 , denotes ,
T11269 595-599 WRB denotes when
T11270 600-609 VBN denotes expressed
T11271 610-612 IN denotes by
T11272 613-622 JJ denotes transient
T11273 623-635 NN denotes transfection
T11274 636-640 IN denotes into
T11275 641-647 NN denotes vector
T11276 648-652 JJ denotes only
T11277 653-658 NNS denotes cells
T11278 658-660 , denotes ,
T11267 660-666 VBD denotes showed
T11279 667-668 DT denotes a
T11281 669-676 JJ denotes diffuse
T11282 677-688 JJ denotes cytoplasmic
T11283 689-701 NN denotes distribution
T11280 702-709 NN denotes pattern
T11284 710-711 -LRB- denotes (
T11286 711-717 NN denotes Figure
T11287 718-720 CD denotes 5C
T11288 720-722 , denotes ,
T11289 722-727 JJ denotes lower
T11285 728-732 JJ denotes left
T11290 732-733 -RRB- denotes )
T11291 733-734 . denotes .
T11292 734-890 sentence denotes When expressed in wild-type AQP2 cells, however, AQP2-F204V-GFP localized to the apical surface to varying degrees (Figure 5C, lower right images [i–iii]).
T11293 735-739 WRB denotes When
T11294 740-749 VBN denotes expressed
T11296 750-752 IN denotes in
T11297 753-757 JJ denotes wild
T11299 757-758 HYPH denotes -
T11298 758-762 NN denotes type
T11301 763-767 NN denotes AQP2
T11300 768-773 NNS denotes cells
T11302 773-775 , denotes ,
T11303 775-782 RB denotes however
T11304 782-784 , denotes ,
T11305 784-788 NN denotes AQP2
T11307 788-789 HYPH denotes -
T11308 789-794 NN denotes F204V
T11309 794-795 HYPH denotes -
T11306 795-798 NN denotes GFP
T11295 799-808 VBD denotes localized
T11310 809-811 IN denotes to
T11311 812-815 DT denotes the
T11313 816-822 JJ denotes apical
T11312 823-830 NN denotes surface
T11314 831-833 IN denotes to
T11315 834-841 VBG denotes varying
T11316 842-849 NNS denotes degrees
T11317 850-851 -LRB- denotes (
T11319 851-857 NN denotes Figure
T11320 858-860 CD denotes 5C
T11321 860-862 , denotes ,
T11322 862-867 JJ denotes lower
T11323 868-873 JJ denotes right
T11318 874-880 NNS denotes images
T11324 881-882 -LRB- denotes [
T11325 882-883 CD denotes i
T11326 883-884 SYM denotes –
T11327 884-887 CD denotes iii
T11328 887-888 -RRB- denotes ]
T11329 888-889 -RRB- denotes )
T11330 889-890 . denotes .
T11331 890-972 sentence denotes The lower right images of Figure 5C shows three cells from a single transfection.
T11332 891-894 DT denotes The
T11334 895-900 JJ denotes lower
T11335 901-906 JJ denotes right
T11333 907-913 NNS denotes images
T11337 914-916 IN denotes of
T11338 917-923 NN denotes Figure
T11339 924-926 CD denotes 5C
T11336 927-932 VBZ denotes shows
T11340 933-938 CD denotes three
T11341 939-944 NNS denotes cells
T11342 945-949 IN denotes from
T11343 950-951 DT denotes a
T11345 952-958 JJ denotes single
T11344 959-971 NN denotes transfection
T11346 971-972 . denotes .
T11347 972-1120 sentence denotes The first is a nontransfected cell that shows the localization of the stably expressing wild-type AQP2, which is apical upon forskolin stimulation.
T11348 973-976 DT denotes The
T11349 977-982 JJ denotes first
T11350 983-985 VBZ denotes is
T11351 986-987 DT denotes a
T11353 988-1002 JJ denotes nontransfected
T11352 1003-1007 NN denotes cell
T11354 1008-1012 WDT denotes that
T11355 1013-1018 VBZ denotes shows
T11356 1019-1022 DT denotes the
T11357 1023-1035 NN denotes localization
T11358 1036-1038 IN denotes of
T11359 1039-1042 DT denotes the
T11361 1043-1049 RB denotes stably
T11362 1050-1060 VBG denotes expressing
T11363 1061-1065 JJ denotes wild
T11365 1065-1066 HYPH denotes -
T11364 1066-1070 NN denotes type
T11360 1071-1075 NN denotes AQP2
T11366 1075-1077 , denotes ,
T11367 1077-1082 WDT denotes which
T11368 1083-1085 VBZ denotes is
T11369 1086-1092 JJ denotes apical
T11370 1093-1097 IN denotes upon
T11371 1098-1107 NN denotes forskolin
T11372 1108-1119 NN denotes stimulation
T11373 1119-1120 . denotes .
T11374 1120-1217 sentence denotes The next two show expression of both the stable wild-type AQP2 and the transient AQP2-F204V-GFP.
T11375 1121-1124 DT denotes The
T11377 1125-1129 JJ denotes next
T11376 1130-1133 CD denotes two
T11378 1134-1138 VBP denotes show
T11379 1139-1149 NN denotes expression
T11380 1150-1152 IN denotes of
T11381 1153-1157 CC denotes both
T11383 1158-1161 DT denotes the
T11384 1162-1168 JJ denotes stable
T11385 1169-1173 JJ denotes wild
T11387 1173-1174 HYPH denotes -
T11386 1174-1178 NN denotes type
T11382 1179-1183 NN denotes AQP2
T11388 1184-1187 CC denotes and
T11389 1188-1191 DT denotes the
T11391 1192-1201 JJ denotes transient
T11392 1202-1206 NN denotes AQP2
T11393 1206-1207 HYPH denotes -
T11394 1207-1212 NN denotes F204V
T11395 1212-1213 HYPH denotes -
T11390 1213-1216 NN denotes GFP
T11396 1216-1217 . denotes .
T11397 1217-1350 sentence denotes In cell (ii), localization of wild-type AQP2 was indistinguishable from AQP2-F204V-GFP; both were apical upon forskolin stimulation.
T11398 1218-1220 IN denotes In
T11400 1221-1225 NN denotes cell
T11401 1226-1227 -LRB- denotes (
T11402 1227-1229 CD denotes ii
T11403 1229-1230 -RRB- denotes )
T11404 1230-1232 , denotes ,
T11405 1232-1244 NN denotes localization
T11406 1245-1247 IN denotes of
T11407 1248-1252 JJ denotes wild
T11409 1252-1253 HYPH denotes -
T11408 1253-1257 NN denotes type
T11410 1258-1262 NN denotes AQP2
T11399 1263-1266 VBD denotes was
T11412 1267-1284 JJ denotes indistinguishable
T11413 1285-1289 IN denotes from
T11414 1290-1294 NN denotes AQP2
T11416 1294-1295 HYPH denotes -
T11417 1295-1300 NN denotes F204V
T11418 1300-1301 HYPH denotes -
T11415 1301-1304 NN denotes GFP
T11419 1304-1305 : denotes ;
T11420 1306-1310 DT denotes both
T11411 1311-1315 VBD denotes were
T11421 1316-1322 JJ denotes apical
T11422 1323-1327 IN denotes upon
T11423 1328-1337 NN denotes forskolin
T11424 1338-1349 NN denotes stimulation
T11425 1349-1350 . denotes .
T11426 1350-1455 sentence denotes Although the effect was subtle in cell (iii), AQP2-F204V-GFP was partly localized to the apical surface.
T11427 1351-1359 IN denotes Although
T11429 1360-1363 DT denotes the
T11430 1364-1370 NN denotes effect
T11428 1371-1374 VBD denotes was
T11432 1375-1381 JJ denotes subtle
T11433 1382-1384 IN denotes in
T11434 1385-1389 NN denotes cell
T11435 1390-1391 -LRB- denotes (
T11436 1391-1394 CD denotes iii
T11437 1394-1395 -RRB- denotes )
T11438 1395-1397 , denotes ,
T11439 1397-1401 NN denotes AQP2
T11441 1401-1402 HYPH denotes -
T11442 1402-1407 NN denotes F204V
T11443 1407-1408 HYPH denotes -
T11440 1408-1411 NN denotes GFP
T11444 1412-1415 VBD denotes was
T11445 1416-1422 RB denotes partly
T11431 1423-1432 VBN denotes localized
T11446 1433-1435 IN denotes to
T11447 1436-1439 DT denotes the
T11449 1440-1446 JJ denotes apical
T11448 1447-1454 NN denotes surface
T11450 1454-1455 . denotes .
T11451 1455-1565 sentence denotes Generally, the localization of AQP2-F204V-GFP was clearly more apical when wild-type AQP2 was also expressed.
T11452 1456-1465 RB denotes Generally
T11454 1465-1467 , denotes ,
T11455 1467-1470 DT denotes the
T11456 1471-1483 NN denotes localization
T11457 1484-1486 IN denotes of
T11458 1487-1491 NN denotes AQP2
T11460 1491-1492 HYPH denotes -
T11461 1492-1497 NN denotes F204V
T11462 1497-1498 HYPH denotes -
T11459 1498-1501 NN denotes GFP
T11453 1502-1505 VBD denotes was
T11463 1506-1513 RB denotes clearly
T11464 1514-1518 RBR denotes more
T11465 1519-1525 JJ denotes apical
T11466 1526-1530 WRB denotes when
T11468 1531-1535 JJ denotes wild
T11470 1535-1536 HYPH denotes -
T11469 1536-1540 NN denotes type
T11471 1541-1545 NN denotes AQP2
T11472 1546-1549 VBD denotes was
T11473 1550-1554 RB denotes also
T11467 1555-1564 VBN denotes expressed
T11474 1564-1565 . denotes .
R2935 T11145 T11146 mark If,interacting
R2938 T11146 T11155 advcl interacting,is
R2940 T11147 T11148 amod wild,type
R2941 T11148 T11150 nmod type,proteins
R2942 T11149 T11148 punct -,type
R2943 T11150 T11146 nsubj proteins,interacting
R2945 T11151 T11148 cc and,type
R2946 T11152 T11148 conj mutant,type
R2947 T11153 T11146 aux are,interacting
R2949 T11154 T11146 advmod indeed,interacting
R2950 T11156 T11146 prep in,interacting
R2952 T11157 T11158 det the,cell
R2953 T11158 T11156 pobj cell,in
R2954 T11159 T11155 punct ", ",is
R2956 T11160 T11161 det this,interaction
R2957 T11161 T11155 nsubj interaction,is
R2958 T11162 T11155 acomp sufficient,is
R2959 T11163 T11164 aux to,rescue
R2961 T11164 T11162 xcomp rescue,sufficient
R2962 T11165 T11166 det the,localization
R2963 T11166 T11164 dobj localization,rescue
R2965 T11167 T11166 prep of,localization
R2966 T11168 T11169 compound mutant,protein
R2967 T11169 T11167 pobj protein,of
R2968 T11170 T11155 punct ?,is
R2970 T11172 T11173 aux To,answer
R2971 T11173 T11174 advcl answer,used
R2973 T11175 T11176 det this,question
R2974 T11176 T11173 dobj question,answer
R2976 T11177 T11174 punct ", ",used
R2977 T11178 T11174 nsubj we,used
R2979 T11179 T11180 compound MDCK,cells
R2980 T11180 T11174 dobj cells,used
R2981 T11181 T11182 advmod stably,transfected
R2982 T11182 T11180 acl transfected,cells
R2983 T11183 T11182 prep with,transfected
R2984 T11184 T11185 amod wild,type
R2986 T11185 T11187 compound type,AQP2
R2987 T11186 T11185 punct -,type
R2988 T11187 T11183 pobj AQP2,with
R2990 T11188 T11187 acl expressing,AQP2
R2991 T11189 T11188 dobj vector,expressing
R2992 T11190 T11188 cc or,expressing
R2994 T11191 T11188 conj with,expressing
R2995 T11192 T11193 amod empty,vector
R2996 T11193 T11191 pobj vector,with
R2998 T11194 T11174 punct .,used
R2999 T11196 T11197 prep On,transfected
R3000 T11203 T11197 advmod transiently,transfected
R3001 T11198 T11196 pobj top,On
R3002 T11199 T11198 prep of,top
R3003 T11200 T11199 pobj these,of
R3004 T11201 T11197 punct ", ",transfected
R3005 T11204 T11205 nmod AQP2,GFP
R3006 T11202 T11197 nsubj we,transfected
R3007 T11205 T11207 nmod GFP,constructs
R3008 T11206 T11205 punct -,GFP
R3009 T11207 T11197 dobj constructs,transfected
R3010 T11208 T11205 cc or,GFP
R3011 T11308 T11306 compound F204V,GFP
R3012 T11309 T11306 punct -,GFP
R3013 T11209 T11210 compound AQP2,GFP
R3014 T11310 T11295 prep to,localized
R3015 T11311 T11312 det the,surface
R3016 T11312 T11310 pobj surface,to
R3017 T11210 T11205 conj GFP,GFP
R3018 T11313 T11312 amod apical,surface
R3019 T11314 T11295 prep to,localized
R3020 T11211 T11210 punct -,GFP
R3021 T11315 T11316 amod varying,degrees
R3022 T11316 T11314 pobj degrees,to
R3023 T11317 T11318 punct (,images
R3024 T11212 T11210 compound F204V,GFP
R3025 T11318 T11295 parataxis images,localized
R3026 T11319 T11318 dep Figure,images
R3027 T11320 T11319 nummod 5C,Figure
R3028 T11213 T11210 punct -,GFP
R3029 T11321 T11318 punct ", ",images
R3030 T11322 T11318 amod lower,images
R3031 T11214 T11207 compound expression,constructs
R3032 T11323 T11318 amod right,images
R3033 T11324 T11325 punct [,i
R3034 T11325 T11318 parataxis i,images
R3035 T11215 T11197 punct .,transfected
R3036 T11326 T11327 punct –,iii
R3037 T11327 T11325 prep iii,i
R3038 T11217 T11218 compound AQP2,GFP
R3039 T11328 T11325 punct ],i
R3040 T11329 T11318 punct ),images
R3041 T11330 T11295 punct .,localized
R3042 T11218 T11220 nsubj GFP,localized
R3043 T11332 T11333 det The,images
R3044 T11333 T11336 nsubj images,shows
R3045 T11219 T11218 punct -,GFP
R3046 T11334 T11335 amod lower,right
R3047 T11335 T11333 amod right,images
R3048 T11221 T11220 prep to,localized
R3049 T11337 T11333 prep of,images
R3050 T11338 T11337 pobj Figure,of
R3051 T11339 T11338 nummod 5C,Figure
R3052 T11222 T11223 det the,surface
R3053 T11340 T11341 nummod three,cells
R3054 T11341 T11336 dobj cells,shows
R3055 T11342 T11341 prep from,cells
R3056 T11223 T11221 pobj surface,to
R3057 T11343 T11344 det a,transfection
R3058 T11344 T11342 pobj transfection,from
R3059 T11345 T11344 amod single,transfection
R3060 T11224 T11223 amod apical,surface
R3061 T11346 T11336 punct .,shows
R3062 T11348 T11349 det The,first
R3063 T11225 T11220 prep upon,localized
R3064 T11349 T11350 nsubj first,is
R3065 T11226 T11227 compound forskolin,stimulation
R3066 T11351 T11352 det a,cell
R3067 T11352 T11350 attr cell,is
R3068 T11353 T11352 amod nontransfected,cell
R3069 T11227 T11225 pobj stimulation,upon
R3070 T11354 T11355 dep that,shows
R3071 T11355 T11352 relcl shows,cell
R3072 T11356 T11357 det the,localization
R3073 T11228 T11229 mark whether,transfected
R3074 T11357 T11355 dobj localization,shows
R3075 T11358 T11357 prep of,localization
R3076 T11359 T11360 det the,AQP2
R3077 T11229 T11220 advcl transfected,localized
R3078 T11360 T11358 pobj AQP2,of
R3079 T11230 T11229 nsubjpass it,transfected
R3080 T11361 T11362 advmod stably,expressing
R3081 T11231 T11229 auxpass was,transfected
R3082 T11362 T11360 amod expressing,AQP2
R3083 T11363 T11364 amod wild,type
R3084 T11232 T11229 advmod transiently,transfected
R3085 T11364 T11360 compound type,AQP2
R3086 T11365 T11364 punct -,type
R3087 T11366 T11360 punct ", ",AQP2
R3088 T11367 T11368 dep which,is
R3089 T11368 T11360 relcl is,AQP2
R3090 T11233 T11229 prep into,transfected
R3091 T11369 T11368 acomp apical,is
R3092 T11370 T11368 prep upon,is
R3093 T11371 T11372 compound forskolin,stimulation
R3094 T11234 T11235 npadvmod vector,only
R3095 T11372 T11370 pobj stimulation,upon
R3096 T11373 T11350 punct .,is
R3097 T11235 T11237 amod only,cells
R3098 T11375 T11376 det The,two
R3099 T11376 T11378 nsubj two,show
R3100 T11377 T11376 amod next,two
R3101 T11236 T11235 punct -,only
R3102 T11379 T11378 dobj expression,show
R3103 T11380 T11379 prep of,expression
R3104 T11237 T11233 pobj cells,into
R3105 T11381 T11382 preconj both,AQP2
R3106 T11382 T11380 pobj AQP2,of
R3107 T11238 T11239 punct (,images
R3108 T11383 T11382 det the,AQP2
R3109 T11384 T11382 amod stable,AQP2
R3110 T11385 T11386 amod wild,type
R3111 T11239 T11237 parataxis images,cells
R3112 T11386 T11382 compound type,AQP2
R3113 T11387 T11386 punct -,type
R3114 T11388 T11382 cc and,AQP2
R3115 T11240 T11239 dep Figure,images
R3116 T11389 T11390 det the,GFP
R3117 T11390 T11382 conj GFP,AQP2
R3118 T11391 T11390 amod transient,GFP
R3119 T11241 T11240 nummod 5C,Figure
R3120 T11392 T11390 compound AQP2,GFP
R3121 T11393 T11390 punct -,GFP
R3122 T11394 T11390 compound F204V,GFP
R3123 T11242 T11239 punct ", ",images
R3124 T11395 T11390 punct -,GFP
R3125 T11396 T11378 punct .,show
R3126 T11243 T11239 amod upper,images
R3127 T11398 T11399 prep In,was
R3128 T11399 T11411 ccomp was,were
R3129 T11244 T11239 amod left,images
R3130 T11400 T11398 pobj cell,In
R3131 T11401 T11400 punct (,cell
R3132 T11402 T11400 nummod ii,cell
R3133 T11403 T11400 punct ),cell
R3134 T11245 T11239 punct ),images
R3135 T11404 T11399 punct ", ",was
R3136 T11405 T11399 nsubj localization,was
R3137 T11406 T11405 prep of,localization
R3138 T11407 T11408 amod wild,type
R3139 T11408 T11410 compound type,AQP2
R3140 T11409 T11408 punct -,type
R3141 T11246 T11233 cc or,into
R3142 T11410 T11406 pobj AQP2,of
R3143 T11412 T11399 acomp indistinguishable,was
R3144 T11247 T11233 conj into,into
R3145 T11248 T11249 amod wild,type
R3146 T11249 T11251 compound type,cells
R3147 T11413 T11412 prep from,indistinguishable
R3148 T11414 T11415 compound AQP2,GFP
R3149 T11250 T11249 punct -,type
R3150 T11251 T11247 pobj cells,into
R3151 T11415 T11413 pobj GFP,from
R3152 T11416 T11415 punct -,GFP
R3153 T11252 T11251 compound AQP2,cells
R3154 T11417 T11415 compound F204V,GFP
R3155 T11418 T11415 punct -,GFP
R3156 T11419 T11411 punct ;,were
R3157 T11253 T11254 punct (,Figure
R3158 T11420 T11411 nsubj both,were
R3159 T11421 T11411 acomp apical,were
R3160 T11254 T11251 parataxis Figure,cells
R3161 T11422 T11411 prep upon,were
R3162 T11423 T11424 compound forskolin,stimulation
R3163 T11255 T11254 nummod 5C,Figure
R3164 T11424 T11422 pobj stimulation,upon
R3165 T11425 T11411 punct .,were
R3166 T11256 T11254 punct ", ",Figure
R3167 T11427 T11428 mark Although,was
R3168 T11428 T11431 advcl was,localized
R3169 T11257 T11258 amod upper,right
R3170 T11429 T11430 det the,effect
R3171 T11430 T11428 nsubj effect,was
R3172 T11258 T11254 amod right,Figure
R3173 T11432 T11428 acomp subtle,was
R3174 T11433 T11428 prep in,was
R3175 T11259 T11254 punct ),Figure
R3176 T11434 T11433 pobj cell,in
R3177 T11435 T11434 punct (,cell
R3178 T11436 T11434 nummod iii,cell
R3179 T11260 T11220 punct .,localized
R3180 T11437 T11434 punct ),cell
R3181 T11438 T11431 punct ", ",localized
R3182 T11439 T11440 compound AQP2,GFP
R3183 T11262 T11263 compound AQP2,GFP
R3184 T11440 T11431 nsubjpass GFP,localized
R3185 T11441 T11440 punct -,GFP
R3186 T11442 T11440 compound F204V,GFP
R3187 T11443 T11440 punct -,GFP
R3188 T11444 T11431 auxpass was,localized
R3189 T11445 T11431 advmod partly,localized
R3190 T11446 T11431 prep to,localized
R3191 T11263 T11267 nsubj GFP,showed
R3192 T11447 T11448 det the,surface
R3193 T11448 T11446 pobj surface,to
R3194 T11449 T11448 amod apical,surface
R3195 T11264 T11263 punct -,GFP
R3196 T11450 T11431 punct .,localized
R3197 T11452 T11453 advmod Generally,was
R3198 T11265 T11263 compound F204V,GFP
R3199 T11454 T11453 punct ", ",was
R3200 T11266 T11263 punct -,GFP
R3201 T11455 T11456 det the,localization
R3202 T11456 T11453 nsubj localization,was
R3203 T11457 T11456 prep of,localization
R3204 T11268 T11267 punct ", ",showed
R3205 T11458 T11459 compound AQP2,GFP
R3206 T11459 T11457 pobj GFP,of
R3207 T11460 T11459 punct -,GFP
R3208 T11461 T11459 compound F204V,GFP
R3209 T11269 T11270 advmod when,expressed
R3210 T11462 T11459 punct -,GFP
R3211 T11463 T11453 advmod clearly,was
R3212 T11464 T11465 advmod more,apical
R3213 T11270 T11267 advcl expressed,showed
R3214 T11465 T11453 acomp apical,was
R3215 T11466 T11467 advmod when,expressed
R3216 T11467 T11453 advcl expressed,was
R3217 T11271 T11270 prep by,expressed
R3218 T11468 T11469 amod wild,type
R3219 T11469 T11471 compound type,AQP2
R3220 T11470 T11469 punct -,type
R3221 T11272 T11273 amod transient,transfection
R3222 T11471 T11467 nsubjpass AQP2,expressed
R3223 T11472 T11467 auxpass was,expressed
R3224 T11473 T11467 advmod also,expressed
R3225 T11273 T11271 pobj transfection,by
R3226 T11474 T11453 punct .,was
R3227 T11274 T11270 prep into,expressed
R3230 T11275 T11276 npadvmod vector,only
R3233 T11276 T11277 amod only,cells
R3237 T11277 T11274 pobj cells,into
R3243 T11278 T11267 punct ", ",showed
R3247 T11279 T11280 det a,pattern
R3251 T11280 T11267 dobj pattern,showed
R3255 T11281 T11280 amod diffuse,pattern
R3259 T11282 T11280 amod cytoplasmic,pattern
R3261 T11283 T11280 compound distribution,pattern
R3263 T11284 T11285 punct (,left
R3267 T11285 T11267 parataxis left,showed
R3270 T11286 T11285 dep Figure,left
R3274 T11287 T11286 nummod 5C,Figure
R3277 T11288 T11285 punct ", ",left
R3281 T11289 T11285 amod lower,left
R3284 T11290 T11285 punct ),left
R3285 T11291 T11267 punct .,showed
R3286 T11293 T11294 advmod When,expressed
R3289 T11294 T11295 advcl expressed,localized
R3294 T11296 T11294 prep in,expressed
R3300 T11297 T11298 amod wild,type
R3304 T11298 T11300 compound type,cells
R3308 T11299 T11298 punct -,type
R3311 T11300 T11296 pobj cells,in
R3315 T11301 T11300 compound AQP2,cells
R3319 T11302 T11295 punct ", ",localized
R3323 T11303 T11295 advmod however,localized
R3327 T11304 T11295 punct ", ",localized
R3331 T11305 T11306 compound AQP2,GFP
R3335 T11306 T11295 nsubj GFP,localized
R3339 T11307 T11306 punct -,GFP