| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T10979 |
0-2 |
IN |
denotes |
In |
| T10981 |
3-6 |
DT |
denotes |
the |
| T10983 |
7-17 |
VBG |
denotes |
collecting |
| T10982 |
18-23 |
NNS |
denotes |
ducts |
| T10984 |
24-28 |
IN |
denotes |
from |
| T10985 |
29-38 |
NN |
denotes |
Aqp2F204V |
| T10987 |
38-39 |
HYPH |
denotes |
/ |
| T10988 |
39-40 |
SYM |
denotes |
+ |
| T10986 |
41-45 |
NNS |
denotes |
mice |
| T10989 |
45-47 |
, |
denotes |
, |
| T10990 |
47-50 |
DT |
denotes |
the |
| T10992 |
51-55 |
JJ |
denotes |
wild |
| T10991 |
56-60 |
NN |
denotes |
type |
| T10993 |
61-64 |
MD |
denotes |
may |
| T10980 |
65-71 |
VB |
denotes |
rescue |
| T10994 |
72-75 |
DT |
denotes |
the |
| T10996 |
76-82 |
NN |
denotes |
mutant |
| T10995 |
83-90 |
NN |
denotes |
protein |
| T10997 |
91-93 |
IN |
denotes |
as |
| T10998 |
94-103 |
VBN |
denotes |
suggested |
| T10999 |
104-106 |
IN |
denotes |
by |
| T11000 |
107-110 |
DT |
denotes |
the |
| T11002 |
111-122 |
JJ |
denotes |
subcellular |
| T11001 |
123-135 |
NN |
denotes |
distribution |
| T11003 |
136-138 |
IN |
denotes |
of |
| T11004 |
139-143 |
NN |
denotes |
AQP2 |
| T11005 |
144-151 |
NN |
denotes |
protein |
| T11006 |
151-152 |
. |
denotes |
. |
| T11007 |
152-283 |
sentence |
denotes |
To test this idea, we first looked for an interaction between mutant and wild-type proteins in transfected MDCK cells (Figure 5B). |
| T11008 |
153-155 |
TO |
denotes |
To |
| T11009 |
156-160 |
VB |
denotes |
test |
| T11011 |
161-165 |
DT |
denotes |
this |
| T11012 |
166-170 |
NN |
denotes |
idea |
| T11013 |
170-172 |
, |
denotes |
, |
| T11014 |
172-174 |
PRP |
denotes |
we |
| T11015 |
175-180 |
RB |
denotes |
first |
| T11010 |
181-187 |
VBD |
denotes |
looked |
| T11016 |
188-191 |
IN |
denotes |
for |
| T11017 |
192-194 |
DT |
denotes |
an |
| T11018 |
195-206 |
NN |
denotes |
interaction |
| T11019 |
207-214 |
IN |
denotes |
between |
| T11020 |
215-221 |
NN |
denotes |
mutant |
| T11022 |
222-225 |
CC |
denotes |
and |
| T11023 |
226-230 |
JJ |
denotes |
wild |
| T11025 |
230-231 |
HYPH |
denotes |
- |
| T11024 |
231-235 |
NN |
denotes |
type |
| T11021 |
236-244 |
NN |
denotes |
proteins |
| T11026 |
245-247 |
IN |
denotes |
in |
| T11027 |
248-259 |
VBN |
denotes |
transfected |
| T11029 |
260-264 |
NN |
denotes |
MDCK |
| T11028 |
265-270 |
NNS |
denotes |
cells |
| T11030 |
271-272 |
-LRB- |
denotes |
( |
| T11031 |
272-278 |
NN |
denotes |
Figure |
| T11032 |
279-281 |
CD |
denotes |
5B |
| T11033 |
281-282 |
-RRB- |
denotes |
) |
| T11034 |
282-283 |
. |
denotes |
. |
| T11035 |
283-449 |
sentence |
denotes |
MDCK cells stably expressing wild-type AQP2 were transiently transfected with GFP expression constructs encoding GFP-tagged wild-type AQP2, AQP2-F204V, or GFP alone. |
| T11036 |
284-288 |
NN |
denotes |
MDCK |
| T11037 |
289-294 |
NNS |
denotes |
cells |
| T11039 |
295-301 |
RB |
denotes |
stably |
| T11040 |
302-312 |
VBG |
denotes |
expressing |
| T11041 |
313-317 |
JJ |
denotes |
wild |
| T11043 |
317-318 |
HYPH |
denotes |
- |
| T11042 |
318-322 |
NN |
denotes |
type |
| T11044 |
323-327 |
NN |
denotes |
AQP2 |
| T11045 |
328-332 |
VBD |
denotes |
were |
| T11046 |
333-344 |
RB |
denotes |
transiently |
| T11038 |
345-356 |
VBN |
denotes |
transfected |
| T11047 |
357-361 |
IN |
denotes |
with |
| T11048 |
362-365 |
NN |
denotes |
GFP |
| T11050 |
366-376 |
NN |
denotes |
expression |
| T11049 |
377-387 |
NNS |
denotes |
constructs |
| T11051 |
388-396 |
VBG |
denotes |
encoding |
| T11052 |
397-400 |
NN |
denotes |
GFP |
| T11054 |
400-401 |
HYPH |
denotes |
- |
| T11053 |
401-407 |
VBN |
denotes |
tagged |
| T11056 |
408-412 |
JJ |
denotes |
wild |
| T11058 |
412-413 |
HYPH |
denotes |
- |
| T11057 |
413-417 |
NN |
denotes |
type |
| T11055 |
418-422 |
NN |
denotes |
AQP2 |
| T11059 |
422-424 |
, |
denotes |
, |
| T11060 |
424-428 |
NN |
denotes |
AQP2 |
| T11062 |
428-429 |
HYPH |
denotes |
- |
| T11061 |
429-434 |
NN |
denotes |
F204V |
| T11063 |
434-436 |
, |
denotes |
, |
| T11064 |
436-438 |
CC |
denotes |
or |
| T11065 |
439-442 |
NN |
denotes |
GFP |
| T11066 |
443-448 |
RB |
denotes |
alone |
| T11067 |
448-449 |
. |
denotes |
. |
| T11068 |
449-699 |
sentence |
denotes |
Antibodies against GFP coimmunoprecipitated wild-type AQP2 when AQP2-GFP or AQP2-F204V-GFP was transiently transfected, but not when GFP by itself was transiently transfected into MDCK cells stably expressing wild-type AQP2 (Figure 5B, upper blots). |
| T11069 |
450-460 |
NNS |
denotes |
Antibodies |
| T11071 |
461-468 |
IN |
denotes |
against |
| T11072 |
469-472 |
NN |
denotes |
GFP |
| T11070 |
473-493 |
VBD |
denotes |
coimmunoprecipitated |
| T11073 |
494-498 |
JJ |
denotes |
wild |
| T11075 |
498-499 |
HYPH |
denotes |
- |
| T11074 |
499-503 |
NN |
denotes |
type |
| T11076 |
504-508 |
NN |
denotes |
AQP2 |
| T11077 |
509-513 |
WRB |
denotes |
when |
| T11079 |
514-518 |
NN |
denotes |
AQP2 |
| T11081 |
518-519 |
HYPH |
denotes |
- |
| T11080 |
519-522 |
NN |
denotes |
GFP |
| T11082 |
523-525 |
CC |
denotes |
or |
| T11083 |
526-530 |
NN |
denotes |
AQP2 |
| T11085 |
530-531 |
HYPH |
denotes |
- |
| T11086 |
531-536 |
NN |
denotes |
F204V |
| T11087 |
536-537 |
HYPH |
denotes |
- |
| T11084 |
537-540 |
NN |
denotes |
GFP |
| T11088 |
541-544 |
VBD |
denotes |
was |
| T11089 |
545-556 |
RB |
denotes |
transiently |
| T11078 |
557-568 |
VBN |
denotes |
transfected |
| T11090 |
568-570 |
, |
denotes |
, |
| T11091 |
570-573 |
CC |
denotes |
but |
| T11092 |
574-577 |
RB |
denotes |
not |
| T11093 |
578-582 |
WRB |
denotes |
when |
| T11095 |
583-586 |
NN |
denotes |
GFP |
| T11096 |
587-589 |
IN |
denotes |
by |
| T11097 |
590-596 |
PRP |
denotes |
itself |
| T11098 |
597-600 |
VBD |
denotes |
was |
| T11099 |
601-612 |
RB |
denotes |
transiently |
| T11094 |
613-624 |
VBN |
denotes |
transfected |
| T11100 |
625-629 |
IN |
denotes |
into |
| T11101 |
630-634 |
NN |
denotes |
MDCK |
| T11102 |
635-640 |
NNS |
denotes |
cells |
| T11103 |
641-647 |
RB |
denotes |
stably |
| T11104 |
648-658 |
VBG |
denotes |
expressing |
| T11105 |
659-663 |
JJ |
denotes |
wild |
| T11107 |
663-664 |
HYPH |
denotes |
- |
| T11106 |
664-668 |
NN |
denotes |
type |
| T11108 |
669-673 |
NN |
denotes |
AQP2 |
| T11109 |
674-675 |
-LRB- |
denotes |
( |
| T11111 |
675-681 |
NN |
denotes |
Figure |
| T11112 |
682-684 |
CD |
denotes |
5B |
| T11113 |
684-686 |
, |
denotes |
, |
| T11114 |
686-691 |
JJ |
denotes |
upper |
| T11110 |
692-697 |
NNS |
denotes |
blots |
| T11115 |
697-698 |
-RRB- |
denotes |
) |
| T11116 |
698-699 |
. |
denotes |
. |
| T11117 |
699-829 |
sentence |
denotes |
Western blot of total membranes showed that wild-type AQP2 is equivalently expressed in all three cases (Figure 5B, lower blots). |
| T11118 |
700-707 |
NNP |
denotes |
Western |
| T11119 |
708-712 |
NN |
denotes |
blot |
| T11121 |
713-715 |
IN |
denotes |
of |
| T11122 |
716-721 |
JJ |
denotes |
total |
| T11123 |
722-731 |
NNS |
denotes |
membranes |
| T11120 |
732-738 |
VBD |
denotes |
showed |
| T11124 |
739-743 |
IN |
denotes |
that |
| T11126 |
744-748 |
JJ |
denotes |
wild |
| T11128 |
748-749 |
HYPH |
denotes |
- |
| T11127 |
749-753 |
NN |
denotes |
type |
| T11129 |
754-758 |
NN |
denotes |
AQP2 |
| T11130 |
759-761 |
VBZ |
denotes |
is |
| T11131 |
762-774 |
RB |
denotes |
equivalently |
| T11125 |
775-784 |
VBN |
denotes |
expressed |
| T11132 |
785-787 |
IN |
denotes |
in |
| T11133 |
788-791 |
DT |
denotes |
all |
| T11135 |
792-797 |
CD |
denotes |
three |
| T11134 |
798-803 |
NNS |
denotes |
cases |
| T11136 |
804-805 |
-LRB- |
denotes |
( |
| T11138 |
805-811 |
NN |
denotes |
Figure |
| T11139 |
812-814 |
CD |
denotes |
5B |
| T11140 |
814-816 |
, |
denotes |
, |
| T11141 |
816-821 |
JJ |
denotes |
lower |
| T11137 |
822-827 |
NNS |
denotes |
blots |
| T11142 |
827-828 |
-RRB- |
denotes |
) |
| T11143 |
828-829 |
. |
denotes |
. |
| R2734 |
T10991 |
T10980 |
nsubj |
type,rescue |
| R2736 |
T10992 |
T10991 |
amod |
wild,type |
| R2738 |
T10993 |
T10980 |
aux |
may,rescue |
| R2739 |
T10994 |
T10995 |
det |
the,protein |
| R2741 |
T10995 |
T10980 |
dobj |
protein,rescue |
| R2742 |
T10996 |
T10995 |
compound |
mutant,protein |
| R2743 |
T10997 |
T10998 |
mark |
as,suggested |
| R2745 |
T10998 |
T10980 |
advcl |
suggested,rescue |
| R2746 |
T10999 |
T10998 |
agent |
by,suggested |
| R2748 |
T11000 |
T11001 |
det |
the,distribution |
| R2749 |
T11001 |
T10999 |
pobj |
distribution,by |
| R2750 |
T11002 |
T11001 |
amod |
subcellular,distribution |
| R2751 |
T11003 |
T11001 |
prep |
of,distribution |
| R2753 |
T11004 |
T11005 |
compound |
AQP2,protein |
| R2754 |
T11005 |
T11003 |
pobj |
protein,of |
| R2756 |
T11006 |
T10980 |
punct |
.,rescue |
| R2757 |
T11008 |
T11009 |
aux |
To,test |
| R2759 |
T11009 |
T11010 |
advcl |
test,looked |
| R2761 |
T11011 |
T11012 |
det |
this,idea |
| R2762 |
T11012 |
T11009 |
dobj |
idea,test |
| R2763 |
T11013 |
T11010 |
punct |
", ",looked |
| R2764 |
T11014 |
T11010 |
nsubj |
we,looked |
| R2766 |
T11015 |
T11010 |
advmod |
first,looked |
| R2767 |
T11016 |
T11010 |
prep |
for,looked |
| R2769 |
T11017 |
T11018 |
det |
an,interaction |
| R2770 |
T11018 |
T11016 |
pobj |
interaction,for |
| R2771 |
T11019 |
T11018 |
prep |
between,interaction |
| R2773 |
T11020 |
T11021 |
nmod |
mutant,proteins |
| R2774 |
T11021 |
T11019 |
pobj |
proteins,between |
| R2775 |
T11022 |
T11020 |
cc |
and,mutant |
| R2776 |
T11023 |
T11024 |
amod |
wild,type |
| R2777 |
T11024 |
T11020 |
conj |
type,mutant |
| R2779 |
T11025 |
T11024 |
punct |
-,type |
| R2780 |
T11026 |
T11010 |
prep |
in,looked |
| R2781 |
T11027 |
T11028 |
amod |
transfected,cells |
| R2783 |
T11028 |
T11026 |
pobj |
cells,in |
| R2784 |
T11029 |
T11028 |
compound |
MDCK,cells |
| R2785 |
T11030 |
T11031 |
punct |
(,Figure |
| R2787 |
T11031 |
T11010 |
parataxis |
Figure,looked |
| R2788 |
T11032 |
T11031 |
nummod |
5B,Figure |
| R2789 |
T11033 |
T11031 |
punct |
),Figure |
| R2790 |
T11034 |
T11010 |
punct |
.,looked |
| R2792 |
T11036 |
T11037 |
compound |
MDCK,cells |
| R2794 |
T11037 |
T11038 |
nsubjpass |
cells,transfected |
| R2795 |
T11039 |
T11040 |
advmod |
stably,expressing |
| R2797 |
T11040 |
T11037 |
acl |
expressing,cells |
| R2798 |
T11041 |
T11042 |
amod |
wild,type |
| R2800 |
T11042 |
T11044 |
compound |
type,AQP2 |
| R2801 |
T11043 |
T11042 |
punct |
-,type |
| R2802 |
T11044 |
T11040 |
dobj |
AQP2,expressing |
| R2804 |
T11045 |
T11038 |
auxpass |
were,transfected |
| R2805 |
T11046 |
T11038 |
advmod |
transiently,transfected |
| R2806 |
T11047 |
T11038 |
prep |
with,transfected |
| R2808 |
T11048 |
T11049 |
compound |
GFP,constructs |
| R2809 |
T11049 |
T11047 |
pobj |
constructs,with |
| R2810 |
T11050 |
T11049 |
compound |
expression,constructs |
| R2812 |
T11051 |
T11049 |
acl |
encoding,constructs |
| R2813 |
T11052 |
T11053 |
npadvmod |
GFP,tagged |
| R2815 |
T11053 |
T11055 |
amod |
tagged,AQP2 |
| R2816 |
T11054 |
T11053 |
punct |
-,tagged |
| R2817 |
T11055 |
T11051 |
dobj |
AQP2,encoding |
| R2819 |
T11056 |
T11057 |
amod |
wild,type |
| R2820 |
T11057 |
T11055 |
compound |
type,AQP2 |
| R2821 |
T11058 |
T11057 |
punct |
-,type |
| R2823 |
T11059 |
T11055 |
punct |
", ",AQP2 |
| R2824 |
T11060 |
T11061 |
compound |
AQP2,F204V |
| R2825 |
T11061 |
T11055 |
conj |
F204V,AQP2 |
| R2826 |
T11062 |
T11061 |
punct |
-,F204V |
| R2827 |
T11063 |
T11061 |
punct |
", ",F204V |
| R2828 |
T11064 |
T11061 |
cc |
or,F204V |
| R2830 |
T11065 |
T11061 |
conj |
GFP,F204V |
| R2831 |
T11066 |
T11065 |
advmod |
alone,GFP |
| R2834 |
T11067 |
T11038 |
punct |
.,transfected |
| R2836 |
T11069 |
T11070 |
nsubj |
Antibodies,coimmunoprecipitated |
| R2837 |
T11071 |
T11069 |
prep |
against,Antibodies |
| R2838 |
T11072 |
T11071 |
pobj |
GFP,against |
| R2840 |
T11073 |
T11074 |
amod |
wild,type |
| R2841 |
T11074 |
T11076 |
compound |
type,AQP2 |
| R2843 |
T11075 |
T11074 |
punct |
-,type |
| R2844 |
T11076 |
T11070 |
dobj |
AQP2,coimmunoprecipitated |
| R2845 |
T11077 |
T11078 |
advmod |
when,transfected |
| R2847 |
T11078 |
T11070 |
advcl |
transfected,coimmunoprecipitated |
| R2848 |
T11079 |
T11080 |
compound |
AQP2,GFP |
| R2850 |
T11080 |
T11078 |
nsubjpass |
GFP,transfected |
| R2851 |
T11081 |
T11080 |
punct |
-,GFP |
| R2852 |
T11082 |
T11080 |
cc |
or,GFP |
| R2854 |
T11083 |
T11084 |
compound |
AQP2,GFP |
| R2855 |
T11084 |
T11080 |
conj |
GFP,GFP |
| R2856 |
T11085 |
T11084 |
punct |
-,GFP |
| R2858 |
T11086 |
T11084 |
compound |
F204V,GFP |
| R2859 |
T11087 |
T11084 |
punct |
-,GFP |
| R2861 |
T11088 |
T11078 |
auxpass |
was,transfected |
| R2862 |
T11089 |
T11078 |
advmod |
transiently,transfected |
| R2863 |
T11090 |
T11078 |
punct |
", ",transfected |
| R2865 |
T11091 |
T11078 |
cc |
but,transfected |
| R2866 |
T11092 |
T11091 |
neg |
not,but |
| R2867 |
T11093 |
T11094 |
advmod |
when,transfected |
| R2869 |
T11094 |
T11078 |
conj |
transfected,transfected |
| R2870 |
T11095 |
T11094 |
nsubjpass |
GFP,transfected |
| R2872 |
T11096 |
T11095 |
prep |
by,GFP |
| R2876 |
T11097 |
T11096 |
pobj |
itself,by |
| R2877 |
T11098 |
T11094 |
auxpass |
was,transfected |
| R2878 |
T11099 |
T11094 |
advmod |
transiently,transfected |
| R2879 |
T11100 |
T11094 |
prep |
into,transfected |
| R2880 |
T11101 |
T11102 |
compound |
MDCK,cells |
| R2882 |
T11102 |
T11100 |
pobj |
cells,into |
| R2883 |
T11103 |
T11104 |
advmod |
stably,expressing |
| R2884 |
T11104 |
T11102 |
acl |
expressing,cells |
| R2886 |
T11105 |
T11106 |
amod |
wild,type |
| R2887 |
T11106 |
T11108 |
compound |
type,AQP2 |
| R2888 |
T11107 |
T11106 |
punct |
-,type |
| R2890 |
T11108 |
T11104 |
dobj |
AQP2,expressing |
| R2891 |
T11109 |
T11110 |
punct |
(,blots |
| R2893 |
T11110 |
T11070 |
parataxis |
blots,coimmunoprecipitated |
| R2894 |
T11111 |
T11110 |
dep |
Figure,blots |
| R2895 |
T11112 |
T11111 |
nummod |
5B,Figure |
| R2897 |
T11113 |
T11110 |
punct |
", ",blots |
| R2898 |
T11114 |
T11110 |
amod |
upper,blots |
| R2899 |
T11115 |
T11110 |
punct |
),blots |
| R2901 |
T11116 |
T11070 |
punct |
.,coimmunoprecipitated |
| R2903 |
T11118 |
T11119 |
compound |
Western,blot |
| R2904 |
T11119 |
T11120 |
nsubj |
blot,showed |
| R2906 |
T11121 |
T11119 |
prep |
of,blot |
| R2907 |
T11122 |
T11123 |
amod |
total,membranes |
| R2908 |
T11123 |
T11121 |
pobj |
membranes,of |
| R2910 |
T11124 |
T11125 |
mark |
that,expressed |
| R2911 |
T11125 |
T11120 |
ccomp |
expressed,showed |
| R2912 |
T11126 |
T11127 |
amod |
wild,type |
| R2914 |
T11127 |
T11129 |
compound |
type,AQP2 |
| R2915 |
T11128 |
T11127 |
punct |
-,type |
| R2916 |
T11129 |
T11125 |
nsubjpass |
AQP2,expressed |
| R2918 |
T11130 |
T11125 |
auxpass |
is,expressed |
| R2919 |
T11131 |
T11125 |
advmod |
equivalently,expressed |
| R2921 |
T11132 |
T11125 |
prep |
in,expressed |
| R2922 |
T11133 |
T11134 |
det |
all,cases |
| R2923 |
T11134 |
T11132 |
pobj |
cases,in |
| R2924 |
T11135 |
T11134 |
nummod |
three,cases |
| R2926 |
T11136 |
T11137 |
punct |
(,blots |
| R2927 |
T11137 |
T11120 |
parataxis |
blots,showed |
| R2928 |
T11138 |
T11137 |
dep |
Figure,blots |
| R2929 |
T11139 |
T11138 |
nummod |
5B,Figure |
| R2930 |
T11140 |
T11137 |
punct |
", ",blots |
| R2931 |
T11141 |
T11137 |
amod |
lower,blots |
| R2933 |
T11142 |
T11137 |
punct |
),blots |
| R2934 |
T11143 |
T11120 |
punct |
.,showed |
| R2955 |
T10979 |
T10980 |
prep |
In,rescue |
| R2960 |
T10981 |
T10982 |
det |
the,ducts |
| R2964 |
T10982 |
T10979 |
pobj |
ducts,In |
| R2969 |
T10983 |
T10982 |
amod |
collecting,ducts |
| R2972 |
T10984 |
T10982 |
prep |
from,ducts |
| R2975 |
T10985 |
T10986 |
nmod |
Aqp2F204V,mice |
| R2978 |
T10986 |
T10984 |
pobj |
mice,from |
| R2985 |
T10987 |
T10985 |
punct |
/,Aqp2F204V |
| R2989 |
T10988 |
T10985 |
punct |
+,Aqp2F204V |
| R2993 |
T10989 |
T10980 |
punct |
", ",rescue |
| R2997 |
T10990 |
T10991 |
det |
the,type |
