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PMC:1183529 / 3014-32885 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T2580 13-23 JJ denotes Congenital
T2582 24-37 JJ denotes diaphragmatic
T2581 38-45 NNS denotes defects
T2583 46-49 VBP denotes are
T2584 50-51 DT denotes a
T2585 52-60 NN denotes spectrum
T2586 61-63 IN denotes of
T2587 64-74 RB denotes relatively
T2588 75-81 JJ denotes common
T2590 82-87 NN denotes birth
T2589 88-95 NNS denotes defects
T2591 95-96 . denotes .
T2592 96-441 sentence denotes The Bochdalek or posterolateral hernias (often referred to as congenital diaphragmatic hernia [CDH]) occur in 1/3,000 live births [1], and although these are the most common type of diaphragmatic defect presenting at birth, diaphragmatic aplasia and diaphragmatic muscularization defects (eventrations) may have a similar clinical presentation.
T2593 97-100 DT denotes The
T2594 101-110 NNP denotes Bochdalek
T2596 111-113 CC denotes or
T2597 114-128 JJ denotes posterolateral
T2598 129-136 NNS denotes hernias
T2599 137-138 -LRB- denotes (
T2600 138-143 RB denotes often
T2601 144-152 VBN denotes referred
T2602 153-155 IN denotes to
T2603 156-158 IN denotes as
T2604 159-169 JJ denotes congenital
T2606 170-183 JJ denotes diaphragmatic
T2605 184-190 NN denotes hernia
T2607 191-192 -LRB- denotes [
T2608 192-195 NN denotes CDH
T2609 195-196 -RRB- denotes ]
T2610 196-197 -RRB- denotes )
T2595 198-203 VBP denotes occur
T2611 204-206 IN denotes in
T2612 207-208 CD denotes 1
T2614 208-209 SYM denotes /
T2613 209-214 CD denotes 3,000
T2616 215-219 JJ denotes live
T2615 220-226 NNS denotes births
T2617 227-228 -LRB- denotes [
T2618 228-229 CD denotes 1
T2619 229-230 -RRB- denotes ]
T2620 230-232 , denotes ,
T2621 232-235 CC denotes and
T2622 236-244 IN denotes although
T2624 245-250 DT denotes these
T2623 251-254 VBP denotes are
T2626 255-258 DT denotes the
T2628 259-263 RBS denotes most
T2629 264-270 JJ denotes common
T2627 271-275 NN denotes type
T2630 276-278 IN denotes of
T2631 279-292 JJ denotes diaphragmatic
T2632 293-299 NN denotes defect
T2633 300-310 VBG denotes presenting
T2634 311-313 IN denotes at
T2635 314-319 NN denotes birth
T2636 319-321 , denotes ,
T2637 321-334 JJ denotes diaphragmatic
T2638 335-342 NN denotes aplasia
T2639 343-346 CC denotes and
T2640 347-360 JJ denotes diaphragmatic
T2642 361-376 NN denotes muscularization
T2641 377-384 NNS denotes defects
T2643 385-386 -LRB- denotes (
T2644 386-398 NNS denotes eventrations
T2645 398-399 -RRB- denotes )
T2646 400-403 MD denotes may
T2625 404-408 VB denotes have
T2647 409-410 DT denotes a
T2649 411-418 JJ denotes similar
T2650 419-427 JJ denotes clinical
T2648 428-440 NN denotes presentation
T2651 440-441 . denotes .
T2652 441-590 sentence denotes Making specific anatomic distinctions among these types of defects can be difficult without direct gross (intraoperative or postmortem) examination.
T2653 442-448 VBG denotes Making
T2655 449-457 JJ denotes specific
T2657 458-466 JJ denotes anatomic
T2656 467-479 NNS denotes distinctions
T2658 480-485 IN denotes among
T2659 486-491 DT denotes these
T2660 492-497 NNS denotes types
T2661 498-500 IN denotes of
T2662 501-508 NNS denotes defects
T2663 509-512 MD denotes can
T2654 513-515 VB denotes be
T2664 516-525 JJ denotes difficult
T2665 526-533 IN denotes without
T2666 534-540 JJ denotes direct
T2668 541-546 JJ denotes gross
T2669 547-548 -LRB- denotes (
T2670 548-562 JJ denotes intraoperative
T2671 563-565 CC denotes or
T2672 566-576 JJ denotes postmortem
T2673 576-577 -RRB- denotes )
T2667 578-589 NN denotes examination
T2674 589-590 . denotes .
T2675 590-694 sentence denotes Pulmonary hypoplasia associated with these diaphragmatic defects causes severe mortality and morbidity.
T2676 591-600 JJ denotes Pulmonary
T2677 601-611 NN denotes hypoplasia
T2679 612-622 JJ denotes associated
T2680 623-627 IN denotes with
T2681 628-633 DT denotes these
T2683 634-647 JJ denotes diaphragmatic
T2682 648-655 NNS denotes defects
T2678 656-662 VBZ denotes causes
T2684 663-669 JJ denotes severe
T2685 670-679 NN denotes mortality
T2686 680-683 CC denotes and
T2687 684-693 NN denotes morbidity
T2688 693-694 . denotes .
T2689 694-816 sentence denotes The pathogenesis and developmental relationship between diaphragmatic defects and pulmonary hypoplasia is not understood.
T2690 695-698 DT denotes The
T2691 699-711 NN denotes pathogenesis
T2693 712-715 CC denotes and
T2694 716-729 JJ denotes developmental
T2695 730-742 NN denotes relationship
T2696 743-750 IN denotes between
T2697 751-764 JJ denotes diaphragmatic
T2698 765-772 NNS denotes defects
T2699 773-776 CC denotes and
T2700 777-786 JJ denotes pulmonary
T2701 787-797 NN denotes hypoplasia
T2702 798-800 VBZ denotes is
T2703 801-804 RB denotes not
T2692 805-815 VBN denotes understood
T2704 815-816 . denotes .
T2705 816-1178 sentence denotes Although advances in the medical management of pulmonary hypoplasia may have decreased the mortality associated with CDH patients who survive to receive care at high-volume centers [2,3], the population-based mortality has been reported to be as great as 62%, and there are a large number of deaths prior to birth or to transfer to a tertiary care facility [4].
T2706 817-825 IN denotes Although
T2708 826-834 NNS denotes advances
T2709 835-837 IN denotes in
T2710 838-841 DT denotes the
T2712 842-849 JJ denotes medical
T2711 850-860 NN denotes management
T2713 861-863 IN denotes of
T2714 864-873 JJ denotes pulmonary
T2715 874-884 NN denotes hypoplasia
T2716 885-888 MD denotes may
T2717 889-893 VB denotes have
T2707 894-903 VBN denotes decreased
T2719 904-907 DT denotes the
T2720 908-917 NN denotes mortality
T2721 918-928 JJ denotes associated
T2722 929-933 IN denotes with
T2723 934-937 NN denotes CDH
T2724 938-946 NNS denotes patients
T2725 947-950 WP denotes who
T2726 951-958 VBP denotes survive
T2727 959-961 TO denotes to
T2728 962-969 VB denotes receive
T2729 970-974 NN denotes care
T2730 975-977 IN denotes at
T2731 978-982 JJ denotes high
T2733 982-983 HYPH denotes -
T2732 983-989 NN denotes volume
T2734 990-997 NNS denotes centers
T2735 998-999 -LRB- denotes [
T2737 999-1000 CD denotes 2
T2738 1000-1001 , denotes ,
T2736 1001-1002 CD denotes 3
T2739 1002-1003 -RRB- denotes ]
T2740 1003-1005 , denotes ,
T2741 1005-1008 DT denotes the
T2743 1009-1019 NN denotes population
T2745 1019-1020 HYPH denotes -
T2744 1020-1025 VBN denotes based
T2742 1026-1035 NN denotes mortality
T2746 1036-1039 VBZ denotes has
T2747 1040-1044 VBN denotes been
T2718 1045-1053 VBN denotes reported
T2748 1054-1056 TO denotes to
T2749 1057-1059 VB denotes be
T2750 1060-1062 RB denotes as
T2752 1063-1068 JJ denotes great
T2753 1069-1071 IN denotes as
T2751 1072-1074 CD denotes 62
T2754 1074-1075 NN denotes %
T2755 1075-1077 , denotes ,
T2756 1077-1080 CC denotes and
T2757 1081-1086 EX denotes there
T2758 1087-1090 VBP denotes are
T2759 1091-1092 DT denotes a
T2761 1093-1098 JJ denotes large
T2760 1099-1105 NN denotes number
T2762 1106-1108 IN denotes of
T2763 1109-1115 NNS denotes deaths
T2764 1116-1121 JJ denotes prior
T2765 1122-1124 IN denotes to
T2766 1125-1130 NN denotes birth
T2767 1131-1133 CC denotes or
T2768 1134-1136 IN denotes to
T2769 1137-1145 NN denotes transfer
T2770 1146-1148 IN denotes to
T2771 1149-1150 DT denotes a
T2773 1151-1159 JJ denotes tertiary
T2774 1160-1164 NN denotes care
T2772 1165-1173 NN denotes facility
T2775 1174-1175 -LRB- denotes [
T2776 1175-1176 CD denotes 4
T2777 1176-1177 -RRB- denotes ]
T2778 1177-1178 . denotes .
T2779 1178-1386 sentence denotes As these patients commonly present with severe respiratory failure at birth, therapy has been centered around developing better methods to provide ventilatory support while not producing further lung injury.
T2780 1179-1181 IN denotes As
T2782 1182-1187 DT denotes these
T2783 1188-1196 NNS denotes patients
T2784 1197-1205 RB denotes commonly
T2781 1206-1213 VBP denotes present
T2786 1214-1218 IN denotes with
T2787 1219-1225 JJ denotes severe
T2789 1226-1237 JJ denotes respiratory
T2788 1238-1245 NN denotes failure
T2790 1246-1248 IN denotes at
T2791 1249-1254 NN denotes birth
T2792 1254-1256 , denotes ,
T2793 1256-1263 NN denotes therapy
T2794 1264-1267 VBZ denotes has
T2795 1268-1272 VBN denotes been
T2785 1273-1281 VBN denotes centered
T2796 1282-1288 IN denotes around
T2797 1289-1299 VBG denotes developing
T2798 1300-1306 JJR denotes better
T2799 1307-1314 NNS denotes methods
T2800 1315-1317 TO denotes to
T2801 1318-1325 VB denotes provide
T2802 1326-1337 JJ denotes ventilatory
T2803 1338-1345 NN denotes support
T2804 1346-1351 IN denotes while
T2806 1352-1355 RB denotes not
T2805 1356-1365 VBG denotes producing
T2807 1366-1373 JJ denotes further
T2809 1374-1378 NN denotes lung
T2808 1379-1385 NN denotes injury
T2810 1385-1386 . denotes .
T2811 1386-1597 sentence denotes Extracorporeal membrane oxygenation (ECMO) is used in some centers to provide an extended period of cardiopulmonary bypass [5,6], while other centers have success using other ventilatory support techniques [7].
T2812 1387-1401 JJ denotes Extracorporeal
T2814 1402-1410 NN denotes membrane
T2813 1411-1422 NN denotes oxygenation
T2816 1423-1424 -LRB- denotes (
T2817 1424-1428 NN denotes ECMO
T2818 1428-1429 -RRB- denotes )
T2819 1430-1432 VBZ denotes is
T2815 1433-1437 VBN denotes used
T2820 1438-1440 IN denotes in
T2821 1441-1445 DT denotes some
T2822 1446-1453 NNS denotes centers
T2823 1454-1456 TO denotes to
T2824 1457-1464 VB denotes provide
T2825 1465-1467 DT denotes an
T2827 1468-1476 JJ denotes extended
T2826 1477-1483 NN denotes period
T2828 1484-1486 IN denotes of
T2829 1487-1502 JJ denotes cardiopulmonary
T2830 1503-1509 NN denotes bypass
T2831 1510-1511 -LRB- denotes [
T2833 1511-1512 CD denotes 5
T2834 1512-1513 , denotes ,
T2832 1513-1514 CD denotes 6
T2835 1514-1515 -RRB- denotes ]
T2836 1515-1517 , denotes ,
T2837 1517-1522 IN denotes while
T2839 1523-1528 JJ denotes other
T2840 1529-1536 NNS denotes centers
T2838 1537-1541 VBP denotes have
T2841 1542-1549 NN denotes success
T2842 1550-1555 VBG denotes using
T2843 1556-1561 JJ denotes other
T2845 1562-1573 JJ denotes ventilatory
T2846 1574-1581 NN denotes support
T2844 1582-1592 NNS denotes techniques
T2847 1593-1594 -LRB- denotes [
T2848 1594-1595 CD denotes 7
T2849 1595-1596 -RRB- denotes ]
T2850 1596-1597 . denotes .
T2851 1597-1887 sentence denotes The morbidity in those who survive is high, and many patients survive with chronic respiratory insufficiency, cognitive and neuromotor deficits, and hearing loss as a result of necessary intensive interventions and associated structural and irreversible developmental abnormalities [8–11].
T2852 1598-1601 DT denotes The
T2853 1602-1611 NN denotes morbidity
T2855 1612-1614 IN denotes in
T2856 1615-1620 DT denotes those
T2857 1621-1624 WP denotes who
T2858 1625-1632 VBP denotes survive
T2854 1633-1635 VBZ denotes is
T2859 1636-1640 JJ denotes high
T2860 1640-1642 , denotes ,
T2861 1642-1645 CC denotes and
T2862 1646-1650 JJ denotes many
T2863 1651-1659 NNS denotes patients
T2864 1660-1667 VBP denotes survive
T2865 1668-1672 IN denotes with
T2866 1673-1680 JJ denotes chronic
T2868 1681-1692 JJ denotes respiratory
T2867 1693-1706 NN denotes insufficiency
T2869 1706-1708 , denotes ,
T2870 1708-1717 JJ denotes cognitive
T2872 1718-1721 CC denotes and
T2873 1722-1732 JJ denotes neuromotor
T2871 1733-1741 NNS denotes deficits
T2874 1741-1743 , denotes ,
T2875 1743-1746 CC denotes and
T2876 1747-1754 NN denotes hearing
T2877 1755-1759 NN denotes loss
T2878 1760-1762 IN denotes as
T2879 1763-1764 DT denotes a
T2880 1765-1771 NN denotes result
T2881 1772-1774 IN denotes of
T2882 1775-1784 JJ denotes necessary
T2884 1785-1794 JJ denotes intensive
T2883 1795-1808 NNS denotes interventions
T2885 1809-1812 CC denotes and
T2886 1813-1823 VBN denotes associated
T2888 1824-1834 JJ denotes structural
T2889 1835-1838 CC denotes and
T2890 1839-1851 JJ denotes irreversible
T2891 1852-1865 JJ denotes developmental
T2887 1866-1879 NNS denotes abnormalities
T2892 1880-1881 -LRB- denotes [
T2893 1881-1882 CD denotes 8
T2894 1882-1883 SYM denotes –
T2895 1883-1885 CD denotes 11
T2896 1885-1886 -RRB- denotes ]
T2897 1886-1887 . denotes .
T2898 1887-2033 sentence denotes To date, there have been no specific mutations found to be associated with nonsyndromic diaphragmatic defects and pulmonary hypoplasia in humans.
T2899 1888-1890 IN denotes To
T2901 1891-1895 NN denotes date
T2902 1895-1897 , denotes ,
T2903 1897-1902 EX denotes there
T2904 1903-1907 VBP denotes have
T2900 1908-1912 VBN denotes been
T2905 1913-1915 DT denotes no
T2907 1916-1924 JJ denotes specific
T2906 1925-1934 NNS denotes mutations
T2908 1935-1940 VBN denotes found
T2909 1941-1943 TO denotes to
T2910 1944-1946 VB denotes be
T2911 1947-1957 JJ denotes associated
T2912 1958-1962 IN denotes with
T2913 1963-1975 JJ denotes nonsyndromic
T2915 1976-1989 JJ denotes diaphragmatic
T2914 1990-1997 NNS denotes defects
T2916 1998-2001 CC denotes and
T2917 2002-2011 JJ denotes pulmonary
T2918 2012-2022 NN denotes hypoplasia
T2919 2023-2025 IN denotes in
T2920 2026-2032 NNS denotes humans
T2921 2032-2033 . denotes .
T2922 2033-2180 sentence denotes The heritability of these defects is unclear, as the high morbidity and mortality limit the collection of multigenerational families for analysis.
T2923 2034-2037 DT denotes The
T2924 2038-2050 NN denotes heritability
T2926 2051-2053 IN denotes of
T2927 2054-2059 DT denotes these
T2928 2060-2067 NNS denotes defects
T2925 2068-2070 VBZ denotes is
T2929 2071-2078 JJ denotes unclear
T2930 2078-2080 , denotes ,
T2931 2080-2082 IN denotes as
T2933 2083-2086 DT denotes the
T2935 2087-2091 JJ denotes high
T2934 2092-2101 NN denotes morbidity
T2936 2102-2105 CC denotes and
T2937 2106-2115 NN denotes mortality
T2932 2116-2121 VBP denotes limit
T2938 2122-2125 DT denotes the
T2939 2126-2136 NN denotes collection
T2940 2137-2139 IN denotes of
T2941 2140-2157 JJ denotes multigenerational
T2942 2158-2166 NNS denotes families
T2943 2167-2170 IN denotes for
T2944 2171-2179 NN denotes analysis
T2945 2179-2180 . denotes .
T2946 2180-2369 sentence denotes The genetic etiologies are likely to be complex and probably arise from different mutations in various parts of the molecular developmental pathways required for diaphragmatic development.
T2947 2181-2184 DT denotes The
T2949 2185-2192 JJ denotes genetic
T2948 2193-2203 NNS denotes etiologies
T2950 2204-2207 VBP denotes are
T2951 2208-2214 JJ denotes likely
T2952 2215-2217 TO denotes to
T2953 2218-2220 VB denotes be
T2954 2221-2228 JJ denotes complex
T2955 2229-2232 CC denotes and
T2956 2233-2241 RB denotes probably
T2957 2242-2247 VBP denotes arise
T2958 2248-2252 IN denotes from
T2959 2253-2262 JJ denotes different
T2960 2263-2272 NNS denotes mutations
T2961 2273-2275 IN denotes in
T2962 2276-2283 JJ denotes various
T2963 2284-2289 NNS denotes parts
T2964 2290-2292 IN denotes of
T2965 2293-2296 DT denotes the
T2967 2297-2306 JJ denotes molecular
T2968 2307-2320 JJ denotes developmental
T2966 2321-2329 NNS denotes pathways
T2969 2330-2338 VBN denotes required
T2970 2339-2342 IN denotes for
T2971 2343-2356 JJ denotes diaphragmatic
T2972 2357-2368 NN denotes development
T2973 2368-2369 . denotes .
T2974 2369-2488 sentence denotes Indeed, there are numerous reports implicating different chromosomal abnormalities in the pathogenesis of CDH [12,13].
T2975 2370-2376 RB denotes Indeed
T2977 2376-2378 , denotes ,
T2978 2378-2383 EX denotes there
T2976 2384-2387 VBP denotes are
T2979 2388-2396 JJ denotes numerous
T2980 2397-2404 NNS denotes reports
T2981 2405-2416 VBG denotes implicating
T2982 2417-2426 JJ denotes different
T2984 2427-2438 JJ denotes chromosomal
T2983 2439-2452 NNS denotes abnormalities
T2985 2453-2455 IN denotes in
T2986 2456-2459 DT denotes the
T2987 2460-2472 NN denotes pathogenesis
T2988 2473-2475 IN denotes of
T2989 2476-2479 NN denotes CDH
T2990 2480-2481 -LRB- denotes [
T2992 2481-2483 CD denotes 12
T2993 2483-2484 , denotes ,
T2991 2484-2486 CD denotes 13
T2994 2486-2487 -RRB- denotes ]
T2995 2487-2488 . denotes .
T2996 2488-2757 sentence denotes Given the difficulty of studying lethal developmental abnormalities in humans, it is of great potential utility to develop animal models of human birth defects, as the specific genetic abnormalities found in animal models can then be investigated in human populations.
T2997 2489-2494 VBN denotes Given
T2999 2495-2498 DT denotes the
T3000 2499-2509 NN denotes difficulty
T3001 2510-2512 IN denotes of
T3002 2513-2521 VBG denotes studying
T3003 2522-2528 JJ denotes lethal
T3005 2529-2542 JJ denotes developmental
T3004 2543-2556 NNS denotes abnormalities
T3006 2557-2559 IN denotes in
T3007 2560-2566 NNS denotes humans
T3008 2566-2568 , denotes ,
T3009 2568-2570 PRP denotes it
T2998 2571-2573 VBZ denotes is
T3010 2574-2576 IN denotes of
T3011 2577-2582 JJ denotes great
T3013 2583-2592 JJ denotes potential
T3012 2593-2600 NN denotes utility
T3014 2601-2603 TO denotes to
T3015 2604-2611 VB denotes develop
T3016 2612-2618 NN denotes animal
T3017 2619-2625 NNS denotes models
T3018 2626-2628 IN denotes of
T3019 2629-2634 JJ denotes human
T3021 2635-2640 NN denotes birth
T3020 2641-2648 NNS denotes defects
T3022 2648-2650 , denotes ,
T3023 2650-2652 IN denotes as
T3025 2653-2656 DT denotes the
T3027 2657-2665 JJ denotes specific
T3028 2666-2673 JJ denotes genetic
T3026 2674-2687 NNS denotes abnormalities
T3029 2688-2693 VBN denotes found
T3030 2694-2696 IN denotes in
T3031 2697-2703 NN denotes animal
T3032 2704-2710 NNS denotes models
T3033 2711-2714 MD denotes can
T3034 2715-2719 RB denotes then
T3035 2720-2722 VB denotes be
T3024 2723-2735 VBN denotes investigated
T3036 2736-2738 IN denotes in
T3037 2739-2744 JJ denotes human
T3038 2745-2756 NNS denotes populations
T3039 2756-2757 . denotes .
T3040 2757-2916 sentence denotes We screened mice treated with the chemical mutagen N-ethyl-N-nitrosourea (ENU) for lines with developmental defects that present in the perinatal period [14].
T3041 2758-2760 PRP denotes We
T3042 2761-2769 VBD denotes screened
T3043 2770-2774 NNS denotes mice
T3044 2775-2782 VBN denotes treated
T3045 2783-2787 IN denotes with
T3046 2788-2791 DT denotes the
T3048 2792-2800 JJ denotes chemical
T3047 2801-2808 NN denotes mutagen
T3049 2809-2810 NN denotes N
T3051 2810-2811 HYPH denotes -
T3050 2811-2816 NN denotes ethyl
T3052 2816-2817 HYPH denotes -
T3053 2817-2818 NN denotes N
T3055 2818-2819 HYPH denotes -
T3054 2819-2830 NN denotes nitrosourea
T3056 2831-2832 -LRB- denotes (
T3057 2832-2835 NN denotes ENU
T3058 2835-2836 -RRB- denotes )
T3059 2837-2840 IN denotes for
T3060 2841-2846 NNS denotes lines
T3061 2847-2851 IN denotes with
T3062 2852-2865 JJ denotes developmental
T3063 2866-2873 NNS denotes defects
T3064 2874-2878 WDT denotes that
T3065 2879-2886 VBP denotes present
T3066 2887-2889 IN denotes in
T3067 2890-2893 DT denotes the
T3069 2894-2903 JJ denotes perinatal
T3068 2904-2910 NN denotes period
T3070 2911-2912 -LRB- denotes [
T3071 2912-2914 CD denotes 14
T3072 2914-2915 -RRB- denotes ]
T3073 2915-2916 . denotes .
T3074 2916-3090 sentence denotes From this screen, we identified a line of mice carrying a recessive mutation that results in primary pulmonary hypoplasia and abnormal diaphragmatic and cardiac development.
T3075 2917-2921 IN denotes From
T3077 2922-2926 DT denotes this
T3078 2927-2933 NN denotes screen
T3079 2933-2935 , denotes ,
T3080 2935-2937 PRP denotes we
T3076 2938-2948 VBD denotes identified
T3081 2949-2950 DT denotes a
T3082 2951-2955 NN denotes line
T3083 2956-2958 IN denotes of
T3084 2959-2963 NNS denotes mice
T3085 2964-2972 VBG denotes carrying
T3086 2973-2974 DT denotes a
T3088 2975-2984 JJ denotes recessive
T3087 2985-2993 NN denotes mutation
T3089 2994-2998 WDT denotes that
T3090 2999-3006 VBZ denotes results
T3091 3007-3009 IN denotes in
T3092 3010-3017 JJ denotes primary
T3094 3018-3027 JJ denotes pulmonary
T3093 3028-3038 NN denotes hypoplasia
T3095 3039-3042 CC denotes and
T3096 3043-3051 JJ denotes abnormal
T3098 3052-3065 JJ denotes diaphragmatic
T3099 3066-3069 CC denotes and
T3100 3070-3077 JJ denotes cardiac
T3097 3078-3089 NN denotes development
T3101 3089-3090 . denotes .
T3102 3090-3295 sentence denotes Positional cloning analysis identified Fog2 (Zfpm2) as a likely candidate, and DNA sequencing revealed a mutation in a splice donor site that generates an abnormal transcript encoding a truncated protein.
T3103 3091-3101 JJ denotes Positional
T3105 3102-3109 NN denotes cloning
T3104 3110-3118 NN denotes analysis
T3106 3119-3129 VBD denotes identified
T3107 3130-3134 NN denotes Fog2
T3108 3135-3136 -LRB- denotes (
T3109 3136-3141 NN denotes Zfpm2
T3110 3141-3142 -RRB- denotes )
T3111 3143-3145 IN denotes as
T3112 3146-3147 DT denotes a
T3114 3148-3154 JJ denotes likely
T3113 3155-3164 NN denotes candidate
T3115 3164-3166 , denotes ,
T3116 3166-3169 CC denotes and
T3117 3170-3173 NN denotes DNA
T3118 3174-3184 NN denotes sequencing
T3119 3185-3193 VBD denotes revealed
T3120 3194-3195 DT denotes a
T3121 3196-3204 NN denotes mutation
T3122 3205-3207 IN denotes in
T3123 3208-3209 DT denotes a
T3125 3210-3216 NN denotes splice
T3126 3217-3222 NN denotes donor
T3124 3223-3227 NN denotes site
T3127 3228-3232 WDT denotes that
T3128 3233-3242 VBZ denotes generates
T3129 3243-3245 DT denotes an
T3131 3246-3254 JJ denotes abnormal
T3130 3255-3265 NN denotes transcript
T3132 3266-3274 VBG denotes encoding
T3133 3275-3276 DT denotes a
T3135 3277-3286 VBN denotes truncated
T3134 3287-3294 NN denotes protein
T3136 3294-3295 . denotes .
T3137 3295-3557 sentence denotes This result suggested that we examine the orthologous gene in humans with similar developmental defects, and we report the finding of a de novo nonsense mutation in FOG2 in a patient who died at birth with a diaphragmatic defect and severe pulmonary hypoplasia.
T3138 3296-3300 DT denotes This
T3139 3301-3307 NN denotes result
T3140 3308-3317 VBD denotes suggested
T3141 3318-3322 IN denotes that
T3143 3323-3325 PRP denotes we
T3142 3326-3333 VBP denotes examine
T3144 3334-3337 DT denotes the
T3146 3338-3349 JJ denotes orthologous
T3145 3350-3354 NN denotes gene
T3147 3355-3357 IN denotes in
T3148 3358-3364 NNS denotes humans
T3149 3365-3369 IN denotes with
T3150 3370-3377 JJ denotes similar
T3152 3378-3391 JJ denotes developmental
T3151 3392-3399 NNS denotes defects
T3153 3399-3401 , denotes ,
T3154 3401-3404 CC denotes and
T3155 3405-3407 PRP denotes we
T3156 3408-3414 VBP denotes report
T3157 3415-3418 DT denotes the
T3158 3419-3426 NN denotes finding
T3159 3427-3429 IN denotes of
T3160 3430-3431 DT denotes a
T3162 3432-3434 FW denotes de
T3163 3435-3439 FW denotes novo
T3164 3440-3448 NN denotes nonsense
T3161 3449-3457 NN denotes mutation
T3165 3458-3460 IN denotes in
T3166 3461-3465 NN denotes FOG2
T3167 3466-3468 IN denotes in
T3168 3469-3470 DT denotes a
T3169 3471-3478 NN denotes patient
T3170 3479-3482 WP denotes who
T3171 3483-3487 VBD denotes died
T3172 3488-3490 IN denotes at
T3173 3491-3496 NN denotes birth
T3174 3497-3501 IN denotes with
T3175 3502-3503 DT denotes a
T3177 3504-3517 JJ denotes diaphragmatic
T3176 3518-3524 NN denotes defect
T3178 3525-3528 CC denotes and
T3179 3529-3535 JJ denotes severe
T3181 3536-3545 JJ denotes pulmonary
T3180 3546-3556 NN denotes hypoplasia
T3182 3556-3557 . denotes .
T3183 3557-3641 sentence denotes This is the first reported mutation associated with these abnormalities in a human.
T3184 3558-3562 DT denotes This
T3185 3563-3565 VBZ denotes is
T3186 3566-3569 DT denotes the
T3188 3570-3575 JJ denotes first
T3189 3576-3584 VBN denotes reported
T3187 3585-3593 NN denotes mutation
T3190 3594-3604 JJ denotes associated
T3191 3605-3609 IN denotes with
T3192 3610-3615 DT denotes these
T3193 3616-3629 NNS denotes abnormalities
T3194 3630-3632 IN denotes in
T3195 3633-3634 DT denotes a
T3196 3635-3640 NN denotes human
T3197 3640-3641 . denotes .
T3198 3641-3781 sentence denotes We present additional data that provide direct evidence that pulmonary hypoplasia may be a primary component of this spectrum of disorders.
T3199 3642-3644 PRP denotes We
T3200 3645-3652 VBP denotes present
T3201 3653-3663 JJ denotes additional
T3202 3664-3668 NNS denotes data
T3203 3669-3673 WDT denotes that
T3204 3674-3681 VBP denotes provide
T3205 3682-3688 JJ denotes direct
T3206 3689-3697 NN denotes evidence
T3207 3698-3702 IN denotes that
T3209 3703-3712 JJ denotes pulmonary
T3210 3713-3723 NN denotes hypoplasia
T3211 3724-3727 MD denotes may
T3208 3728-3730 VB denotes be
T3212 3731-3732 DT denotes a
T3214 3733-3740 JJ denotes primary
T3213 3741-3750 NN denotes component
T3215 3751-3753 IN denotes of
T3216 3754-3758 DT denotes this
T3217 3759-3767 NN denotes spectrum
T3218 3768-3770 IN denotes of
T3219 3771-3780 NNS denotes disorders
T3220 3780-3781 . denotes .
T5356 3792-3806 NN denotes Identification
T5357 3807-3809 IN denotes of
T5358 3810-3813 DT denotes the
T5360 3814-3820 JJ denotes little
T5361 3821-3825 NN denotes lung
T5359 3826-3834 NN denotes Mutation
T5362 3835-3837 IN denotes in
T5363 3838-3842 NN denotes Fog2
T5364 3842-3977 sentence denotes We screened third-generation progeny of ENU-mutagenized mice at embryonic day 18.5 (E18.5) for abnormal developmental phenotypes [14].
T5365 3843-3845 PRP denotes We
T5366 3846-3854 VBD denotes screened
T5367 3855-3860 JJ denotes third
T5369 3860-3861 HYPH denotes -
T5368 3861-3871 NN denotes generation
T5370 3872-3879 NN denotes progeny
T5371 3880-3882 IN denotes of
T5372 3883-3886 NN denotes ENU
T5374 3886-3887 HYPH denotes -
T5373 3887-3898 VBN denotes mutagenized
T5375 3899-3903 NNS denotes mice
T5376 3904-3906 IN denotes at
T5377 3907-3916 JJ denotes embryonic
T5378 3917-3920 NN denotes day
T5379 3921-3925 CD denotes 18.5
T5380 3926-3927 -LRB- denotes (
T5381 3927-3932 NN denotes E18.5
T5382 3932-3933 -RRB- denotes )
T5383 3934-3937 IN denotes for
T5384 3938-3946 JJ denotes abnormal
T5386 3947-3960 JJ denotes developmental
T5385 3961-3971 NNS denotes phenotypes
T5387 3972-3973 -LRB- denotes [
T5388 3973-3975 CD denotes 14
T5389 3975-3976 -RRB- denotes ]
T5390 3976-3977 . denotes .
T5391 3977-4110 sentence denotes One line of mice was found to have multiple embryos in independent litters that displayed pulmonary hypoplasia and a thin diaphragm.
T5392 3978-3981 CD denotes One
T5393 3982-3986 NN denotes line
T5395 3987-3989 IN denotes of
T5396 3990-3994 NNS denotes mice
T5397 3995-3998 VBD denotes was
T5394 3999-4004 VBN denotes found
T5398 4005-4007 TO denotes to
T5399 4008-4012 VB denotes have
T5400 4013-4021 JJ denotes multiple
T5401 4022-4029 NNS denotes embryos
T5402 4030-4032 IN denotes in
T5403 4033-4044 JJ denotes independent
T5404 4045-4052 NNS denotes litters
T5405 4053-4057 WDT denotes that
T5406 4058-4067 VBD denotes displayed
T5407 4068-4077 JJ denotes pulmonary
T5408 4078-4088 NN denotes hypoplasia
T5409 4089-4092 CC denotes and
T5410 4093-4094 DT denotes a
T5412 4095-4099 JJ denotes thin
T5411 4100-4109 NN denotes diaphragm
T5413 4109-4110 . denotes .
T5414 4110-4265 sentence denotes The mutation, which we called little lung (lil), was mapped to Chromosome 15 by utilizing a strategy of interval haplotype analysis (data not shown) [15].
T5415 4111-4114 DT denotes The
T5416 4115-4123 NN denotes mutation
T5418 4123-4125 , denotes ,
T5419 4125-4130 WDT denotes which
T5421 4131-4133 PRP denotes we
T5420 4134-4140 VBD denotes called
T5422 4141-4147 JJ denotes little
T5423 4148-4152 NN denotes lung
T5424 4153-4154 -LRB- denotes (
T5425 4154-4157 NN denotes lil
T5426 4157-4158 -RRB- denotes )
T5427 4158-4160 , denotes ,
T5428 4160-4163 VBD denotes was
T5417 4164-4170 VBN denotes mapped
T5429 4171-4173 IN denotes to
T5430 4174-4184 NN denotes Chromosome
T5431 4185-4187 CD denotes 15
T5432 4188-4190 IN denotes by
T5433 4191-4200 VBG denotes utilizing
T5434 4201-4202 DT denotes a
T5435 4203-4211 NN denotes strategy
T5436 4212-4214 IN denotes of
T5437 4215-4223 JJ denotes interval
T5439 4224-4233 NN denotes haplotype
T5438 4234-4242 NN denotes analysis
T5440 4243-4244 -LRB- denotes (
T5442 4244-4248 NNS denotes data
T5443 4249-4252 RB denotes not
T5441 4253-4258 VBN denotes shown
T5444 4258-4259 -RRB- denotes )
T5445 4260-4261 -LRB- denotes [
T5446 4261-4263 CD denotes 15
T5447 4263-4264 -RRB- denotes ]
T5448 4264-4265 . denotes .
T5449 4265-4337 sentence denotes For high-resolution mapping, F2 progeny from two crosses were analyzed.
T5450 4266-4269 IN denotes For
T5452 4270-4274 JJ denotes high
T5454 4274-4275 HYPH denotes -
T5453 4275-4285 NN denotes resolution
T5455 4286-4293 NN denotes mapping
T5456 4293-4295 , denotes ,
T5457 4295-4297 NN denotes F2
T5458 4298-4305 NN denotes progeny
T5459 4306-4310 IN denotes from
T5460 4311-4314 CD denotes two
T5461 4315-4322 NNS denotes crosses
T5462 4323-4327 VBD denotes were
T5451 4328-4336 VBN denotes analyzed
T5463 4336-4337 . denotes .
T5464 4337-4502 sentence denotes In 450 progeny of an intercross of F1 (A/J × FVB/N) lil/+ mice, the interval containing the mutation was defined by 19 recombinants between D15Mit220 and D15Mit154.
T5465 4338-4340 IN denotes In
T5467 4341-4344 CD denotes 450
T5468 4345-4352 NN denotes progeny
T5469 4353-4355 IN denotes of
T5470 4356-4358 DT denotes an
T5471 4359-4369 NN denotes intercross
T5472 4370-4372 IN denotes of
T5473 4373-4375 NN denotes F1
T5475 4376-4377 -LRB- denotes (
T5477 4377-4378 NN denotes A
T5478 4378-4379 SYM denotes /
T5476 4379-4380 NN denotes J
T5479 4381-4382 SYM denotes ×
T5480 4383-4386 NN denotes FVB
T5482 4386-4387 HYPH denotes /
T5481 4387-4388 NN denotes N
T5483 4388-4389 -RRB- denotes )
T5484 4390-4393 NN denotes lil
T5485 4393-4394 HYPH denotes /
T5486 4394-4395 SYM denotes +
T5474 4396-4400 NNS denotes mice
T5487 4400-4402 , denotes ,
T5488 4402-4405 DT denotes the
T5489 4406-4414 NN denotes interval
T5490 4415-4425 VBG denotes containing
T5491 4426-4429 DT denotes the
T5492 4430-4438 NN denotes mutation
T5493 4439-4442 VBD denotes was
T5466 4443-4450 VBN denotes defined
T5494 4451-4453 IN denotes by
T5495 4454-4456 CD denotes 19
T5496 4457-4469 NNS denotes recombinants
T5497 4470-4477 IN denotes between
T5498 4478-4487 NN denotes D15Mit220
T5499 4488-4491 CC denotes and
T5500 4492-4501 NN denotes D15Mit154
T5501 4501-4502 . denotes .
T5502 4502-4637 sentence denotes Because of the lack of informative markers within this interval, an additional 39 F2 progeny from an A/J × C57BL/6J cross were tested.
T5503 4503-4510 IN denotes Because
T5505 4511-4513 IN denotes of
T5506 4514-4517 DT denotes the
T5507 4518-4522 NN denotes lack
T5508 4523-4525 IN denotes of
T5509 4526-4537 JJ denotes informative
T5510 4538-4545 NNS denotes markers
T5511 4546-4552 IN denotes within
T5512 4553-4557 DT denotes this
T5513 4558-4566 NN denotes interval
T5514 4566-4568 , denotes ,
T5515 4568-4570 DT denotes an
T5517 4571-4581 JJ denotes additional
T5516 4582-4584 CD denotes 39
T5519 4585-4587 NN denotes F2
T5518 4588-4595 NN denotes progeny
T5520 4596-4600 IN denotes from
T5521 4601-4603 DT denotes an
T5523 4604-4605 NN denotes A
T5525 4605-4606 HYPH denotes /
T5524 4606-4607 NN denotes J
T5526 4608-4609 SYM denotes ×
T5527 4610-4615 NN denotes C57BL
T5529 4615-4616 HYPH denotes /
T5528 4616-4618 NN denotes 6J
T5522 4619-4624 NN denotes cross
T5530 4625-4629 VBD denotes were
T5504 4630-4636 VBN denotes tested
T5531 4636-4637 . denotes .
T5532 4637-4757 sentence denotes The identification of two recombinants established D15Mit85 as the proximal and D15Mit5 as the distal flanking markers.
T5533 4638-4641 DT denotes The
T5534 4642-4656 NN denotes identification
T5536 4657-4659 IN denotes of
T5537 4660-4663 CD denotes two
T5538 4664-4676 NNS denotes recombinants
T5535 4677-4688 VBD denotes established
T5539 4689-4697 NN denotes D15Mit85
T5540 4698-4700 IN denotes as
T5541 4701-4704 DT denotes the
T5542 4705-4713 JJ denotes proximal
T5543 4714-4717 CC denotes and
T5544 4718-4725 NN denotes D15Mit5
T5545 4726-4728 IN denotes as
T5546 4729-4732 DT denotes the
T5547 4733-4739 JJ denotes distal
T5548 4740-4748 NN denotes flanking
T5549 4749-4756 NNS denotes markers
T5550 4756-4757 . denotes .
T5551 4757-4865 sentence denotes The lil phenotype was identified at E18.5 to have bilateral pulmonary hypoplasia and an abnormal diaphragm.
T5552 4758-4761 DT denotes The
T5554 4762-4765 NN denotes lil
T5553 4766-4775 NN denotes phenotype
T5556 4776-4779 VBD denotes was
T5555 4780-4790 VBN denotes identified
T5557 4791-4793 IN denotes at
T5558 4794-4799 NN denotes E18.5
T5559 4800-4802 TO denotes to
T5560 4803-4807 VB denotes have
T5561 4808-4817 JJ denotes bilateral
T5563 4818-4827 JJ denotes pulmonary
T5562 4828-4838 NN denotes hypoplasia
T5564 4839-4842 CC denotes and
T5565 4843-4845 DT denotes an
T5567 4846-4854 JJ denotes abnormal
T5566 4855-4864 NN denotes diaphragm
T5568 4864-4865 . denotes .
T5569 4865-4942 sentence denotes Pulmonary hypoplasia was apparent in all mutant mice that survived to birth.
T5570 4866-4875 JJ denotes Pulmonary
T5571 4876-4886 NN denotes hypoplasia
T5572 4887-4890 VBD denotes was
T5573 4891-4899 JJ denotes apparent
T5574 4900-4902 IN denotes in
T5575 4903-4906 DT denotes all
T5577 4907-4913 NN denotes mutant
T5576 4914-4918 NNS denotes mice
T5578 4919-4923 WDT denotes that
T5579 4924-4932 VBD denotes survived
T5580 4933-4935 IN denotes to
T5581 4936-4941 NN denotes birth
T5582 4941-4942 . denotes .
T5583 4942-5127 sentence denotes In a comparison between wild-type and mutant mice found dead on day one of life, body weights were not different; however, the lung weights were significantly lower in the mutant mice.
T5584 4943-4945 IN denotes In
T5586 4946-4947 DT denotes a
T5587 4948-4958 NN denotes comparison
T5588 4959-4966 IN denotes between
T5589 4967-4971 JJ denotes wild
T5591 4971-4972 HYPH denotes -
T5590 4972-4976 NN denotes type
T5593 4977-4980 CC denotes and
T5594 4981-4987 NN denotes mutant
T5592 4988-4992 NNS denotes mice
T5595 4993-4998 VBN denotes found
T5596 4999-5003 JJ denotes dead
T5597 5004-5006 IN denotes on
T5598 5007-5010 NN denotes day
T5599 5011-5014 CD denotes one
T5600 5015-5017 IN denotes of
T5601 5018-5022 NN denotes life
T5602 5022-5024 , denotes ,
T5603 5024-5028 NN denotes body
T5604 5029-5036 NNS denotes weights
T5605 5037-5041 VBD denotes were
T5606 5042-5045 RB denotes not
T5607 5046-5055 JJ denotes different
T5608 5055-5056 : denotes ;
T5609 5057-5064 RB denotes however
T5610 5064-5066 , denotes ,
T5611 5066-5069 DT denotes the
T5613 5070-5074 NN denotes lung
T5612 5075-5082 NNS denotes weights
T5585 5083-5087 VBD denotes were
T5614 5088-5101 RB denotes significantly
T5615 5102-5107 JJR denotes lower
T5616 5108-5110 IN denotes in
T5617 5111-5114 DT denotes the
T5619 5115-5121 NN denotes mutant
T5618 5122-5126 NNS denotes mice
T5620 5126-5127 . denotes .
T5621 5127-5246 sentence denotes The average mutant lung weight was 9.6 ± 2.5 mg while the average wild-type lung weight was 26.4 ± 4.6 mg (p < 0.001).
T5622 5128-5131 DT denotes The
T5624 5132-5139 JJ denotes average
T5625 5140-5146 NN denotes mutant
T5626 5147-5151 NN denotes lung
T5623 5152-5158 NN denotes weight
T5627 5159-5162 VBD denotes was
T5628 5163-5166 CD denotes 9.6
T5630 5167-5168 SYM denotes ±
T5629 5169-5172 CD denotes 2.5
T5631 5173-5175 NN denotes mg
T5632 5176-5181 IN denotes while
T5634 5182-5185 DT denotes the
T5636 5186-5193 JJ denotes average
T5637 5194-5198 JJ denotes wild
T5639 5198-5199 HYPH denotes -
T5638 5199-5203 NN denotes type
T5640 5204-5208 NN denotes lung
T5635 5209-5215 NN denotes weight
T5633 5216-5219 VBD denotes was
T5641 5220-5224 CD denotes 26.4
T5643 5225-5226 SYM denotes ±
T5642 5227-5230 CD denotes 4.6
T5644 5231-5233 NN denotes mg
T5645 5234-5235 -LRB- denotes (
T5647 5235-5236 NN denotes p
T5648 5237-5238 SYM denotes <
T5646 5239-5244 CD denotes 0.001
T5649 5244-5245 -RRB- denotes )
T5650 5245-5246 . denotes .
T5651 5246-5394 sentence denotes All lungs from mutant mice were lacking an accessory lobe on the right side and had underdevelopment of the anterior right middle lobe (Figure 1A).
T5652 5247-5250 DT denotes All
T5653 5251-5256 NNS denotes lungs
T5655 5257-5261 IN denotes from
T5656 5262-5268 NN denotes mutant
T5657 5269-5273 NNS denotes mice
T5658 5274-5278 VBD denotes were
T5654 5279-5286 VBG denotes lacking
T5659 5287-5289 DT denotes an
T5661 5290-5299 JJ denotes accessory
T5660 5300-5304 NN denotes lobe
T5662 5305-5307 IN denotes on
T5663 5308-5311 DT denotes the
T5665 5312-5317 JJ denotes right
T5664 5318-5322 NN denotes side
T5666 5323-5326 CC denotes and
T5667 5327-5330 VBD denotes had
T5668 5331-5347 NN denotes underdevelopment
T5669 5348-5350 IN denotes of
T5670 5351-5354 DT denotes the
T5672 5355-5363 JJ denotes anterior
T5673 5364-5369 JJ denotes right
T5674 5370-5376 JJ denotes middle
T5671 5377-5381 NN denotes lobe
T5675 5382-5383 -LRB- denotes (
T5676 5383-5389 NN denotes Figure
T5677 5390-5392 CD denotes 1A
T5678 5392-5393 -RRB- denotes )
T5679 5393-5394 . denotes .
T5680 5394-5493 sentence denotes Diaphragms from mutant lil mice were intact, but muscularization was absent in the dorsal regions.
T5681 5395-5405 NNS denotes Diaphragms
T5683 5406-5410 IN denotes from
T5684 5411-5417 NN denotes mutant
T5686 5418-5421 NN denotes lil
T5685 5422-5426 NNS denotes mice
T5682 5427-5431 VBD denotes were
T5687 5432-5438 JJ denotes intact
T5688 5438-5440 , denotes ,
T5689 5440-5443 CC denotes but
T5690 5444-5459 NN denotes muscularization
T5691 5460-5463 VBD denotes was
T5692 5464-5470 JJ denotes absent
T5693 5471-5473 IN denotes in
T5694 5474-5477 DT denotes the
T5696 5478-5484 JJ denotes dorsal
T5695 5485-5492 NNS denotes regions
T5697 5492-5493 . denotes .
T5698 5493-5557 sentence denotes Myotubules were present in a limited and abnormal distribution.
T5699 5494-5504 NNS denotes Myotubules
T5700 5505-5509 VBD denotes were
T5701 5510-5517 JJ denotes present
T5702 5518-5520 IN denotes in
T5703 5521-5522 DT denotes a
T5705 5523-5530 JJ denotes limited
T5706 5531-5534 CC denotes and
T5707 5535-5543 JJ denotes abnormal
T5704 5544-5556 NN denotes distribution
T5708 5556-5557 . denotes .
T5709 5557-5734 sentence denotes More specifically, myotubules normally radiate in a mediolateral fashion to meet the lateral body walls, with a normal paucity of muscle fibers in the central tendonous region.
T5710 5558-5562 RBR denotes More
T5711 5563-5575 RB denotes specifically
T5713 5575-5577 , denotes ,
T5714 5577-5587 NNS denotes myotubules
T5715 5588-5596 RB denotes normally
T5712 5597-5604 VBP denotes radiate
T5716 5605-5607 IN denotes in
T5717 5608-5609 DT denotes a
T5719 5610-5622 JJ denotes mediolateral
T5718 5623-5630 NN denotes fashion
T5720 5631-5633 TO denotes to
T5721 5634-5638 VB denotes meet
T5722 5639-5642 DT denotes the
T5724 5643-5650 JJ denotes lateral
T5725 5651-5655 NN denotes body
T5723 5656-5661 NNS denotes walls
T5726 5661-5663 , denotes ,
T5727 5663-5667 IN denotes with
T5728 5668-5669 DT denotes a
T5730 5670-5676 JJ denotes normal
T5729 5677-5684 NN denotes paucity
T5731 5685-5687 IN denotes of
T5732 5688-5694 NN denotes muscle
T5733 5695-5701 NNS denotes fibers
T5734 5702-5704 IN denotes in
T5735 5705-5708 DT denotes the
T5737 5709-5716 JJ denotes central
T5738 5717-5726 JJ denotes tendonous
T5736 5727-5733 NN denotes region
T5739 5733-5734 . denotes .
T5740 5734-5893 sentence denotes In the mutant diaphragm, myotubules radiated in a dorsal–ventral orientation, and muscle tissue did not meet the entire surface of the body walls (Figure 1B).
T5741 5735-5737 IN denotes In
T5743 5738-5741 DT denotes the
T5745 5742-5748 NN denotes mutant
T5744 5749-5758 NN denotes diaphragm
T5746 5758-5760 , denotes ,
T5747 5760-5770 NNS denotes myotubules
T5742 5771-5779 VBN denotes radiated
T5748 5780-5782 IN denotes in
T5749 5783-5784 DT denotes a
T5751 5785-5791 JJ denotes dorsal
T5753 5791-5792 HYPH denotes –
T5752 5792-5799 JJ denotes ventral
T5750 5800-5811 NN denotes orientation
T5754 5811-5813 , denotes ,
T5755 5813-5816 CC denotes and
T5756 5817-5823 NN denotes muscle
T5757 5824-5830 NN denotes tissue
T5759 5831-5834 VBD denotes did
T5760 5835-5838 RB denotes not
T5758 5839-5843 VB denotes meet
T5761 5844-5847 DT denotes the
T5763 5848-5854 JJ denotes entire
T5762 5855-5862 NN denotes surface
T5764 5863-5865 IN denotes of
T5765 5866-5869 DT denotes the
T5767 5870-5874 NN denotes body
T5766 5875-5880 NNS denotes walls
T5768 5881-5882 -LRB- denotes (
T5769 5882-5888 NN denotes Figure
T5770 5889-5891 CD denotes 1B
T5771 5891-5892 -RRB- denotes )
T5772 5892-5893 . denotes .
T5773 5893-6492 sentence denotes Figure 1 Abnormal Pulmonary and Diaphragmatic Development in the lil Mouse (A) The mutant hypoplastic lung (right) lacks the development of the accessory lobe and the anterior portion of the right middle lobe (marked with arrows on the control sample on the left). (B) Whole diaphragms show a lack of normal muscularization in the posterolateral regions and the peripheral regions of the mutant diaphragm (control on left and lil diaphragm on the right). The number of lil mutant mice that survived to birth was less than 5% of total progeny, rather than the 25% expected for a recessive mutation.
T5774 6350-6353 DT denotes The
T5775 6354-6360 NN denotes number
T5777 6361-6363 IN denotes of
T5778 6364-6367 NN denotes lil
T5780 6368-6374 NN denotes mutant
T5779 6375-6379 NNS denotes mice
T5781 6380-6384 WDT denotes that
T5782 6385-6393 VBD denotes survived
T5783 6394-6396 IN denotes to
T5784 6397-6402 NN denotes birth
T5776 6403-6406 VBD denotes was
T5785 6407-6411 JJR denotes less
T5787 6412-6416 IN denotes than
T5786 6417-6418 CD denotes 5
T5788 6418-6419 NN denotes %
T5789 6420-6422 IN denotes of
T5790 6423-6428 JJ denotes total
T5791 6429-6436 NN denotes progeny
T5792 6436-6438 , denotes ,
T5793 6438-6444 RB denotes rather
T5794 6445-6449 IN denotes than
T5795 6450-6453 DT denotes the
T5797 6454-6456 CD denotes 25
T5796 6456-6457 NN denotes %
T5798 6458-6466 VBN denotes expected
T5799 6467-6470 IN denotes for
T5800 6471-6472 DT denotes a
T5802 6473-6482 JJ denotes recessive
T5801 6483-6491 NN denotes mutation
T5803 6491-6492 . denotes .
T5804 6492-6621 sentence denotes We evaluated litters at different embryonic time points to determine whether the reduced number was due to intra-uterine demise.
T5805 6493-6495 PRP denotes We
T5806 6496-6505 VBD denotes evaluated
T5807 6506-6513 NNS denotes litters
T5808 6514-6516 IN denotes at
T5809 6517-6526 JJ denotes different
T5811 6527-6536 JJ denotes embryonic
T5812 6537-6541 NN denotes time
T5810 6542-6548 NNS denotes points
T5813 6549-6551 TO denotes to
T5814 6552-6561 VB denotes determine
T5815 6562-6569 IN denotes whether
T5817 6570-6573 DT denotes the
T5819 6574-6581 VBN denotes reduced
T5818 6582-6588 NN denotes number
T5816 6589-6592 VBD denotes was
T5820 6593-6596 IN denotes due
T5821 6597-6599 IN denotes to
T5822 6600-6613 JJ denotes intra-uterine
T5823 6614-6620 NN denotes demise
T5824 6620-6621 . denotes .
T5825 6621-6822 sentence denotes Litters were collected at E12.5, 13.5, 15.5, 17.5, and 18.5, and embryos were genotyped and evaluated for evidence of intra-uterine demise, including growth retardation, pallor, and tissue friability.
T5826 6622-6629 NNS denotes Litters
T5828 6630-6634 VBD denotes were
T5827 6635-6644 VBN denotes collected
T5829 6645-6647 IN denotes at
T5830 6648-6653 NN denotes E12.5
T5831 6653-6655 , denotes ,
T5832 6655-6659 CD denotes 13.5
T5833 6659-6661 , denotes ,
T5834 6661-6665 CD denotes 15.5
T5835 6665-6667 , denotes ,
T5836 6667-6671 CD denotes 17.5
T5837 6671-6673 , denotes ,
T5838 6673-6676 CC denotes and
T5839 6677-6681 CD denotes 18.5
T5840 6681-6683 , denotes ,
T5841 6683-6686 CC denotes and
T5842 6687-6694 NNS denotes embryos
T5844 6695-6699 VBD denotes were
T5843 6700-6709 VBN denotes genotyped
T5845 6710-6713 CC denotes and
T5846 6714-6723 VBN denotes evaluated
T5847 6724-6727 IN denotes for
T5848 6728-6736 NN denotes evidence
T5849 6737-6739 IN denotes of
T5850 6740-6753 JJ denotes intra-uterine
T5851 6754-6760 NN denotes demise
T5852 6760-6762 , denotes ,
T5853 6762-6771 VBG denotes including
T5854 6772-6778 NN denotes growth
T5855 6779-6790 NN denotes retardation
T5856 6790-6792 , denotes ,
T5857 6792-6798 NN denotes pallor
T5858 6798-6800 , denotes ,
T5859 6800-6803 CC denotes and
T5860 6804-6810 NN denotes tissue
T5861 6811-6821 NN denotes friability
T5862 6821-6822 . denotes .
T5863 6822-6901 sentence denotes lil embryos had a progressively higher rate of demise between E13.5 and E15.5.
T5864 6823-6826 NN denotes lil
T5865 6827-6834 NNS denotes embryos
T5866 6835-6838 VBD denotes had
T5867 6839-6840 DT denotes a
T5869 6841-6854 RB denotes progressively
T5870 6855-6861 JJR denotes higher
T5868 6862-6866 NN denotes rate
T5871 6867-6869 IN denotes of
T5872 6870-6876 NN denotes demise
T5873 6877-6884 IN denotes between
T5874 6885-6890 NN denotes E13.5
T5875 6891-6894 CC denotes and
T5876 6895-6900 NN denotes E15.5
T5877 6900-6901 . denotes .
T5878 6901-6998 sentence denotes At E12.5, 20 out of 87 (23%) were homozygous for the mutation, and all of these appeared viable.
T5879 6902-6904 IN denotes At
T5881 6905-6910 NN denotes E12.5
T5882 6910-6912 , denotes ,
T5883 6912-6914 CD denotes 20
T5885 6915-6918 IN denotes out
T5886 6919-6921 IN denotes of
T5884 6922-6924 CD denotes 87
T5887 6925-6926 -LRB- denotes (
T5889 6926-6928 CD denotes 23
T5888 6928-6929 NN denotes %
T5890 6929-6930 -RRB- denotes )
T5880 6931-6935 VBD denotes were
T5891 6936-6946 JJ denotes homozygous
T5892 6947-6950 IN denotes for
T5893 6951-6954 DT denotes the
T5894 6955-6963 NN denotes mutation
T5895 6963-6965 , denotes ,
T5896 6965-6968 CC denotes and
T5897 6969-6972 DT denotes all
T5899 6973-6975 IN denotes of
T5900 6976-6981 DT denotes these
T5898 6982-6990 VBD denotes appeared
T5901 6991-6997 JJ denotes viable
T5902 6997-6998 . denotes .
T5903 6998-7053 sentence denotes At E13.5, 13 of 49 (27%) were mutant and two had died.
T5904 6999-7001 IN denotes At
T5906 7002-7007 NN denotes E13.5
T5907 7007-7009 , denotes ,
T5908 7009-7011 CD denotes 13
T5910 7012-7014 IN denotes of
T5909 7015-7017 CD denotes 49
T5911 7018-7019 -LRB- denotes (
T5913 7019-7021 CD denotes 27
T5912 7021-7022 NN denotes %
T5914 7022-7023 -RRB- denotes )
T5905 7024-7028 VBD denotes were
T5915 7029-7035 JJ denotes mutant
T5916 7036-7039 CC denotes and
T5917 7040-7043 CD denotes two
T5919 7044-7047 VBD denotes had
T5918 7048-7052 VBN denotes died
T5920 7052-7053 . denotes .
T5921 7053-7158 sentence denotes At E15.5, 22 out of 91 (24%) were mutant and the majority of mutant embryos (17 out of the 22) had died.
T5922 7054-7056 IN denotes At
T5924 7057-7062 NN denotes E15.5
T5925 7062-7064 , denotes ,
T5926 7064-7066 CD denotes 22
T5928 7067-7070 IN denotes out
T5929 7071-7073 IN denotes of
T5927 7074-7076 CD denotes 91
T5930 7077-7078 -LRB- denotes (
T5932 7078-7080 CD denotes 24
T5931 7080-7081 NN denotes %
T5933 7081-7082 -RRB- denotes )
T5923 7083-7087 VBD denotes were
T5934 7088-7094 JJ denotes mutant
T5935 7095-7098 CC denotes and
T5936 7099-7102 DT denotes the
T5937 7103-7111 NN denotes majority
T5939 7112-7114 IN denotes of
T5940 7115-7121 NN denotes mutant
T5941 7122-7129 NNS denotes embryos
T5942 7130-7131 -LRB- denotes (
T5944 7131-7133 CD denotes 17
T5945 7134-7137 IN denotes out
T5946 7138-7140 IN denotes of
T5947 7141-7144 DT denotes the
T5943 7145-7147 CD denotes 22
T5948 7147-7148 -RRB- denotes )
T5949 7149-7152 VBD denotes had
T5938 7153-7157 VBN denotes died
T5950 7157-7158 . denotes .
T5951 7158-7264 sentence denotes By E18.5, nine of 29 embryos (31%) were homozygous for the mutation, of which only one embryo was viable.
T5952 7159-7161 IN denotes By
T5954 7162-7167 NN denotes E18.5
T5955 7167-7169 , denotes ,
T5956 7169-7173 CD denotes nine
T5958 7174-7176 IN denotes of
T5957 7177-7179 CD denotes 29
T5959 7180-7187 NNS denotes embryos
T5960 7188-7189 -LRB- denotes (
T5962 7189-7191 CD denotes 31
T5961 7191-7192 NN denotes %
T5963 7192-7193 -RRB- denotes )
T5953 7194-7198 VBD denotes were
T5964 7199-7209 JJ denotes homozygous
T5965 7210-7213 IN denotes for
T5966 7214-7217 DT denotes the
T5967 7218-7226 NN denotes mutation
T5968 7226-7228 , denotes ,
T5969 7228-7230 IN denotes of
T5971 7231-7236 WDT denotes which
T5972 7237-7241 RB denotes only
T5974 7242-7245 CD denotes one
T5973 7246-7252 NN denotes embryo
T5970 7253-7256 VBD denotes was
T5975 7257-7263 JJ denotes viable
T5976 7263-7264 . denotes .
T5977 7264-7345 sentence denotes Diaphragmatic muscularization was abnormal in all mutant mice examined (n > 25).
T5978 7265-7278 JJ denotes Diaphragmatic
T5979 7279-7294 NN denotes muscularization
T5980 7295-7298 VBD denotes was
T5981 7299-7307 JJ denotes abnormal
T5982 7308-7310 IN denotes in
T5983 7311-7314 DT denotes all
T5985 7315-7321 NN denotes mutant
T5984 7322-7326 NNS denotes mice
T5986 7327-7335 VBN denotes examined
T5987 7336-7337 -LRB- denotes (
T5989 7337-7338 NN denotes n
T5990 7339-7340 SYM denotes >
T5988 7341-7343 CD denotes 25
T5991 7343-7344 -RRB- denotes )
T5992 7344-7345 . denotes .
T5993 7345-7461 sentence denotes Pulmonary hypoplasia was observed in 100% of mutants evaluated for that phenotype between E11.5 and birth (n > 50).
T5994 7346-7355 JJ denotes Pulmonary
T5995 7356-7366 NN denotes hypoplasia
T5997 7367-7370 VBD denotes was
T5996 7371-7379 VBN denotes observed
T5998 7380-7382 IN denotes in
T5999 7383-7386 CD denotes 100
T6000 7386-7387 NN denotes %
T6001 7388-7390 IN denotes of
T6002 7391-7398 NNS denotes mutants
T6003 7399-7408 VBN denotes evaluated
T6004 7409-7412 IN denotes for
T6005 7413-7417 DT denotes that
T6006 7418-7427 NN denotes phenotype
T6007 7428-7435 IN denotes between
T6008 7436-7441 NN denotes E11.5
T6009 7442-7445 CC denotes and
T6010 7446-7451 NN denotes birth
T6011 7452-7453 -LRB- denotes (
T6013 7453-7454 NN denotes n
T6014 7455-7456 SYM denotes >
T6012 7457-7459 CD denotes 50
T6015 7459-7460 -RRB- denotes )
T6016 7460-7461 . denotes .
T6017 7461-7719 sentence denotes Examination of cardiac morphology showed that hearts from E15.5 lil mutant mice had a variety of developmental defects, including enlarged and abnormally developed endocardial cushions, a double-outlet right ventricle, and a complete atrioventricular canal.
T6018 7462-7473 NN denotes Examination
T6020 7474-7476 IN denotes of
T6021 7477-7484 JJ denotes cardiac
T6022 7485-7495 NN denotes morphology
T6019 7496-7502 VBD denotes showed
T6023 7503-7507 IN denotes that
T6025 7508-7514 NNS denotes hearts
T6026 7515-7519 IN denotes from
T6027 7520-7525 NN denotes E15.5
T6029 7526-7529 NN denotes lil
T6030 7530-7536 NN denotes mutant
T6028 7537-7541 NNS denotes mice
T6024 7542-7545 VBD denotes had
T6031 7546-7547 DT denotes a
T6032 7548-7555 NN denotes variety
T6033 7556-7558 IN denotes of
T6034 7559-7572 JJ denotes developmental
T6035 7573-7580 NNS denotes defects
T6036 7580-7582 , denotes ,
T6037 7582-7591 VBG denotes including
T6038 7592-7600 VBN denotes enlarged
T6040 7601-7604 CC denotes and
T6041 7605-7615 RB denotes abnormally
T6042 7616-7625 VBN denotes developed
T6043 7626-7637 JJ denotes endocardial
T6039 7638-7646 NNS denotes cushions
T6044 7646-7648 , denotes ,
T6045 7648-7649 DT denotes a
T6047 7650-7656 JJ denotes double
T6049 7656-7657 HYPH denotes -
T6048 7657-7663 NN denotes outlet
T6050 7664-7669 JJ denotes right
T6046 7670-7679 NN denotes ventricle
T6051 7679-7681 , denotes ,
T6052 7681-7684 CC denotes and
T6053 7685-7686 DT denotes a
T6055 7687-7695 JJ denotes complete
T6056 7696-7712 JJ denotes atrioventricular
T6054 7713-7718 NN denotes canal
T6057 7718-7719 . denotes .
T6058 7719-7829 sentence denotes The myocardium was also poorly developed, with thinning of the outer compact layer and decreased vascularity.
T6059 7720-7723 DT denotes The
T6060 7724-7734 NN denotes myocardium
T6061 7735-7738 VBD denotes was
T6062 7739-7743 RB denotes also
T6063 7744-7750 RB denotes poorly
T6064 7751-7760 VBN denotes developed
T6065 7760-7762 , denotes ,
T6066 7762-7766 IN denotes with
T6067 7767-7775 NN denotes thinning
T6068 7776-7778 IN denotes of
T6069 7779-7782 DT denotes the
T6071 7783-7788 JJ denotes outer
T6072 7789-7796 JJ denotes compact
T6070 7797-7802 NN denotes layer
T6073 7803-7806 CC denotes and
T6074 7807-7816 VBN denotes decreased
T6075 7817-7828 NN denotes vascularity
T6076 7828-7829 . denotes .
T6077 7829-8049 sentence denotes The cohort of mutant mice that survived to birth also had cardiac malformations including atrioventricular-canal-type ventricular septal defects, ostium primum atrial septal defects, and enlarged atria (data not shown).
T6078 7830-7833 DT denotes The
T6079 7834-7840 NN denotes cohort
T6081 7841-7843 IN denotes of
T6082 7844-7850 NN denotes mutant
T6083 7851-7855 NNS denotes mice
T6084 7856-7860 WDT denotes that
T6085 7861-7869 VBD denotes survived
T6086 7870-7872 IN denotes to
T6087 7873-7878 NN denotes birth
T6088 7879-7883 RB denotes also
T6080 7884-7887 VBD denotes had
T6089 7888-7895 JJ denotes cardiac
T6090 7896-7909 NNS denotes malformations
T6091 7910-7919 VBG denotes including
T6092 7920-7936 JJ denotes atrioventricular
T6094 7936-7937 HYPH denotes -
T6093 7937-7942 NN denotes canal
T6096 7942-7943 HYPH denotes -
T6095 7943-7947 NN denotes type
T6098 7948-7959 JJ denotes ventricular
T6099 7960-7966 JJ denotes septal
T6097 7967-7974 NNS denotes defects
T6100 7974-7976 , denotes ,
T6101 7976-7982 NN denotes ostium
T6103 7983-7989 NN denotes primum
T6104 7990-7996 JJ denotes atrial
T6105 7997-8003 JJ denotes septal
T6102 8004-8011 NNS denotes defects
T6106 8011-8013 , denotes ,
T6107 8013-8016 CC denotes and
T6108 8017-8025 VBN denotes enlarged
T6109 8026-8031 NNS denotes atria
T6110 8032-8033 -LRB- denotes (
T6112 8033-8037 NNS denotes data
T6113 8038-8041 RB denotes not
T6111 8042-8047 VBN denotes shown
T6114 8047-8048 -RRB- denotes )
T6115 8048-8049 . denotes .
T6116 8049-8151 sentence denotes All mutants specifically evaluated for a cardiac phenotype (n = 10) had abnormal cardiac development.
T6117 8050-8053 DT denotes All
T6118 8054-8061 NNS denotes mutants
T6120 8062-8074 RB denotes specifically
T6121 8075-8084 VBN denotes evaluated
T6122 8085-8088 IN denotes for
T6123 8089-8090 DT denotes a
T6125 8091-8098 JJ denotes cardiac
T6124 8099-8108 NN denotes phenotype
T6126 8109-8110 -LRB- denotes (
T6128 8110-8111 NN denotes n
T6129 8112-8113 SYM denotes =
T6127 8114-8116 CD denotes 10
T6130 8116-8117 -RRB- denotes )
T6119 8118-8121 VBD denotes had
T6131 8122-8130 JJ denotes abnormal
T6133 8131-8138 JJ denotes cardiac
T6132 8139-8150 NN denotes development
T6134 8150-8151 . denotes .
T6135 8151-8306 sentence denotes Examination of the 3-Mb region between D15Mit85 and D15Mit5 in DNA sequence databases revealed three predicted genes and four known genes, including Fog2.
T6136 8152-8163 NN denotes Examination
T6138 8164-8166 IN denotes of
T6139 8167-8170 DT denotes the
T6141 8171-8172 CD denotes 3
T6143 8172-8173 HYPH denotes -
T6142 8173-8175 NN denotes Mb
T6140 8176-8182 NN denotes region
T6144 8183-8190 IN denotes between
T6145 8191-8199 NN denotes D15Mit85
T6146 8200-8203 CC denotes and
T6147 8204-8211 NN denotes D15Mit5
T6148 8212-8214 IN denotes in
T6149 8215-8218 NN denotes DNA
T6150 8219-8227 NN denotes sequence
T6151 8228-8237 NNS denotes databases
T6137 8238-8246 VBD denotes revealed
T6152 8247-8252 CD denotes three
T6154 8253-8262 VBN denotes predicted
T6153 8263-8268 NNS denotes genes
T6155 8269-8272 CC denotes and
T6156 8273-8277 CD denotes four
T6158 8278-8283 JJ denotes known
T6157 8284-8289 NNS denotes genes
T6159 8289-8291 , denotes ,
T6160 8291-8300 VBG denotes including
T6161 8301-8305 NN denotes Fog2
T6162 8305-8306 . denotes .
T6163 8306-8516 sentence denotes Targeted mutations of Fog2 have cardiac defects strikingly similar to those we identified in lil mutant mice, including atrioventricular canal defects, thinned myocardium, and absent coronary vasculature [16].
T6164 8307-8315 VBN denotes Targeted
T6165 8316-8325 NNS denotes mutations
T6167 8326-8328 IN denotes of
T6168 8329-8333 NN denotes Fog2
T6166 8334-8338 VBP denotes have
T6169 8339-8346 JJ denotes cardiac
T6170 8347-8354 NNS denotes defects
T6171 8355-8365 RB denotes strikingly
T6172 8366-8373 JJ denotes similar
T6173 8374-8376 IN denotes to
T6174 8377-8382 DT denotes those
T6175 8383-8385 PRP denotes we
T6176 8386-8396 VBD denotes identified
T6177 8397-8399 IN denotes in
T6178 8400-8403 NN denotes lil
T6180 8404-8410 NN denotes mutant
T6179 8411-8415 NNS denotes mice
T6181 8415-8417 , denotes ,
T6182 8417-8426 VBG denotes including
T6183 8427-8443 JJ denotes atrioventricular
T6185 8444-8449 NN denotes canal
T6184 8450-8457 NNS denotes defects
T6186 8457-8459 , denotes ,
T6187 8459-8466 VBN denotes thinned
T6188 8467-8477 NN denotes myocardium
T6189 8477-8479 , denotes ,
T6190 8479-8482 CC denotes and
T6191 8483-8489 JJ denotes absent
T6193 8490-8498 JJ denotes coronary
T6192 8499-8510 NN denotes vasculature
T6194 8511-8512 -LRB- denotes [
T6195 8512-8514 CD denotes 16
T6196 8514-8515 -RRB- denotes ]
T6197 8515-8516 . denotes .
T6198 8516-8658 sentence denotes RT-PCR amplification of the proximal portion of Fog2 revealed longer transcripts in the lil mutant than in the wild-type embryos (Figure 2A).
T6199 8517-8519 NN denotes RT
T6201 8519-8520 HYPH denotes -
T6200 8520-8523 NN denotes PCR
T6202 8524-8537 NN denotes amplification
T6204 8538-8540 IN denotes of
T6205 8541-8544 DT denotes the
T6207 8545-8553 JJ denotes proximal
T6206 8554-8561 NN denotes portion
T6208 8562-8564 IN denotes of
T6209 8565-8569 NN denotes Fog2
T6203 8570-8578 VBD denotes revealed
T6210 8579-8585 JJR denotes longer
T6211 8586-8597 NNS denotes transcripts
T6212 8598-8600 IN denotes in
T6213 8601-8604 DT denotes the
T6215 8605-8608 NN denotes lil
T6214 8609-8615 NN denotes mutant
T6216 8616-8620 IN denotes than
T6217 8621-8623 IN denotes in
T6218 8624-8627 DT denotes the
T6220 8628-8632 JJ denotes wild
T6221 8632-8633 HYPH denotes -
T6219 8633-8637 NN denotes type
T6222 8638-8645 NNS denotes embryos
T6223 8646-8647 -LRB- denotes (
T6224 8647-8653 NN denotes Figure
T6225 8654-8656 CD denotes 2A
T6226 8656-8657 -RRB- denotes )
T6227 8657-8658 . denotes .
T6228 8658-8836 sentence denotes Sequencing of the mutant transcript revealed a point mutation (from thymine to cytosine) 2 bp after position 301, which is in the splice donor site at the end of the third exon.
T6229 8659-8669 NN denotes Sequencing
T6231 8670-8672 IN denotes of
T6232 8673-8676 DT denotes the
T6234 8677-8683 NN denotes mutant
T6233 8684-8694 NN denotes transcript
T6230 8695-8703 VBD denotes revealed
T6235 8704-8705 DT denotes a
T6237 8706-8711 NN denotes point
T6236 8712-8720 NN denotes mutation
T6238 8721-8722 -LRB- denotes (
T6239 8722-8726 IN denotes from
T6240 8727-8734 NN denotes thymine
T6241 8735-8737 IN denotes to
T6242 8738-8746 NN denotes cytosine
T6243 8746-8747 -RRB- denotes )
T6244 8748-8749 CD denotes 2
T6245 8750-8752 NN denotes bp
T6246 8753-8758 IN denotes after
T6247 8759-8767 NN denotes position
T6248 8768-8771 CD denotes 301
T6249 8771-8773 , denotes ,
T6250 8773-8778 WDT denotes which
T6251 8779-8781 VBZ denotes is
T6252 8782-8784 IN denotes in
T6253 8785-8788 DT denotes the
T6255 8789-8795 NN denotes splice
T6256 8796-8801 NN denotes donor
T6254 8802-8806 NN denotes site
T6257 8807-8809 IN denotes at
T6258 8810-8813 DT denotes the
T6259 8814-8817 NN denotes end
T6260 8818-8820 IN denotes of
T6261 8821-8824 DT denotes the
T6263 8825-8830 JJ denotes third
T6262 8831-8835 NN denotes exon
T6264 8835-8836 . denotes .
T6265 8836-9056 sentence denotes This mutation causes a splicing defect that results in the insertion of 85 bp of intronic sequence into the mutant transcript, and introduces a stop codon that generates a severely truncated protein product (Figure 2B).
T6266 8837-8841 DT denotes This
T6267 8842-8850 NN denotes mutation
T6268 8851-8857 VBZ denotes causes
T6269 8858-8859 DT denotes a
T6271 8860-8868 NN denotes splicing
T6270 8869-8875 NN denotes defect
T6272 8876-8880 WDT denotes that
T6273 8881-8888 VBZ denotes results
T6274 8889-8891 IN denotes in
T6275 8892-8895 DT denotes the
T6276 8896-8905 NN denotes insertion
T6277 8906-8908 IN denotes of
T6278 8909-8911 CD denotes 85
T6279 8912-8914 NN denotes bp
T6280 8915-8917 IN denotes of
T6281 8918-8926 JJ denotes intronic
T6282 8927-8935 NN denotes sequence
T6283 8936-8940 IN denotes into
T6284 8941-8944 DT denotes the
T6286 8945-8951 NN denotes mutant
T6285 8952-8962 NN denotes transcript
T6287 8962-8964 , denotes ,
T6288 8964-8967 CC denotes and
T6289 8968-8978 VBZ denotes introduces
T6290 8979-8980 DT denotes a
T6292 8981-8985 NN denotes stop
T6291 8986-8991 NN denotes codon
T6293 8992-8996 WDT denotes that
T6294 8997-9006 VBZ denotes generates
T6295 9007-9008 DT denotes a
T6297 9009-9017 RB denotes severely
T6298 9018-9027 VBN denotes truncated
T6299 9028-9035 NN denotes protein
T6296 9036-9043 NN denotes product
T6300 9044-9045 -LRB- denotes (
T6301 9045-9051 NN denotes Figure
T6302 9052-9054 CD denotes 2B
T6303 9054-9055 -RRB- denotes )
T6304 9055-9056 . denotes .
T6305 9056-9169 sentence denotes Heterozygous lil mutant mice were crossed with a Fog2 +/− (null allele) mutant generated by gene targeting [16].
T6306 9057-9069 JJ denotes Heterozygous
T6308 9070-9073 NN denotes lil
T6309 9074-9080 NN denotes mutant
T6307 9081-9085 NNS denotes mice
T6311 9086-9090 VBD denotes were
T6310 9091-9098 VBN denotes crossed
T6312 9099-9103 IN denotes with
T6313 9104-9105 DT denotes a
T6315 9106-9110 NN denotes Fog2
T6316 9111-9112 SYM denotes +
T6317 9112-9113 HYPH denotes /
T6318 9113-9114 SYM denotes −
T6319 9115-9116 -LRB- denotes (
T6321 9116-9120 JJ denotes null
T6320 9121-9127 NN denotes allele
T6322 9127-9128 -RRB- denotes )
T6314 9129-9135 NN denotes mutant
T6323 9136-9145 VBN denotes generated
T6324 9146-9148 IN denotes by
T6325 9149-9153 NN denotes gene
T6326 9154-9163 NN denotes targeting
T6327 9164-9165 -LRB- denotes [
T6328 9165-9167 CD denotes 16
T6329 9167-9168 -RRB- denotes ]
T6330 9168-9169 . denotes .
T6331 9169-9295 sentence denotes Doubly heterozygous mice had an embryonic lethal phenotype; this failure to complement proves that lil is a mutation in Fog2.
T6332 9170-9176 RB denotes Doubly
T6333 9177-9189 JJ denotes heterozygous
T6334 9190-9194 NNS denotes mice
T6335 9195-9198 VBD denotes had
T6337 9199-9201 DT denotes an
T6339 9202-9211 JJ denotes embryonic
T6340 9212-9218 JJ denotes lethal
T6338 9219-9228 NN denotes phenotype
T6341 9228-9229 : denotes ;
T6342 9230-9234 DT denotes this
T6343 9235-9242 NN denotes failure
T6344 9243-9245 TO denotes to
T6345 9246-9256 VB denotes complement
T6336 9257-9263 VBZ denotes proves
T6346 9264-9268 IN denotes that
T6348 9269-9272 NN denotes lil
T6347 9273-9275 VBZ denotes is
T6349 9276-9277 DT denotes a
T6350 9278-9286 NN denotes mutation
T6351 9287-9289 IN denotes in
T6352 9290-9294 NN denotes Fog2
T6353 9294-9295 . denotes .
T6354 9295-9498 sentence denotes The variable phenotype of lil mice (relative to that found for the Fog2 null mutant) is likely due to the generation of a low level of normal transcript despite the presence of the splice site mutation.
T6355 9296-9299 DT denotes The
T6357 9300-9308 JJ denotes variable
T6356 9309-9318 NN denotes phenotype
T6359 9319-9321 IN denotes of
T6360 9322-9325 NN denotes lil
T6361 9326-9330 NNS denotes mice
T6362 9331-9332 -LRB- denotes (
T6363 9332-9340 JJ denotes relative
T6364 9341-9343 IN denotes to
T6365 9344-9348 DT denotes that
T6366 9349-9354 VBN denotes found
T6367 9355-9358 IN denotes for
T6368 9359-9362 DT denotes the
T6370 9363-9367 NN denotes Fog2
T6371 9368-9372 JJ denotes null
T6369 9373-9379 NN denotes mutant
T6372 9379-9380 -RRB- denotes )
T6358 9381-9383 VBZ denotes is
T6373 9384-9390 RB denotes likely
T6374 9391-9394 IN denotes due
T6375 9395-9397 IN denotes to
T6376 9398-9401 DT denotes the
T6377 9402-9412 NN denotes generation
T6378 9413-9415 IN denotes of
T6379 9416-9417 DT denotes a
T6381 9418-9421 JJ denotes low
T6380 9422-9427 NN denotes level
T6382 9428-9430 IN denotes of
T6383 9431-9437 JJ denotes normal
T6384 9438-9448 NN denotes transcript
T6385 9449-9456 IN denotes despite
T6386 9457-9460 DT denotes the
T6387 9461-9469 NN denotes presence
T6388 9470-9472 IN denotes of
T6389 9473-9476 DT denotes the
T6391 9477-9483 NN denotes splice
T6392 9484-9488 NN denotes site
T6390 9489-9497 NN denotes mutation
T6393 9497-9498 . denotes .
T8121 9924-9928 NN denotes Lung
T8123 9929-9932 CC denotes and
T8124 9933-9942 NN denotes Diaphragm
T8122 9943-9954 NN denotes Development
T8125 9955-9957 IN denotes in
T8126 9958-9961 DT denotes the
T8128 9962-9966 NN denotes Fog2
T8129 9967-9973 NN denotes Mutant
T8127 9974-9979 NN denotes Mouse
T8130 9979-10079 sentence denotes Experiments were conducted to evaluate the role of Fog2 in pulmonary and diaphragmatic development.
T8131 9980-9991 NNS denotes Experiments
T8133 9992-9996 VBD denotes were
T8132 9997-10006 VBN denotes conducted
T8134 10007-10009 TO denotes to
T8135 10010-10018 VB denotes evaluate
T8136 10019-10022 DT denotes the
T8137 10023-10027 NN denotes role
T8138 10028-10030 IN denotes of
T8139 10031-10035 NN denotes Fog2
T8140 10036-10038 IN denotes in
T8141 10039-10048 JJ denotes pulmonary
T8143 10049-10052 CC denotes and
T8144 10053-10066 JJ denotes diaphragmatic
T8142 10067-10078 NN denotes development
T8145 10078-10079 . denotes .
T8146 10079-10222 sentence denotes The pulmonary phenotype is characterized by diffuse hypoplasia and specific loss of the accessory lobe and a portion of the right middle lobe.
T8147 10080-10083 DT denotes The
T8149 10084-10093 JJ denotes pulmonary
T8148 10094-10103 NN denotes phenotype
T8151 10104-10106 VBZ denotes is
T8150 10107-10120 VBN denotes characterized
T8152 10121-10123 IN denotes by
T8153 10124-10131 JJ denotes diffuse
T8154 10132-10142 NN denotes hypoplasia
T8155 10143-10146 CC denotes and
T8156 10147-10155 JJ denotes specific
T8157 10156-10160 NN denotes loss
T8158 10161-10163 IN denotes of
T8159 10164-10167 DT denotes the
T8161 10168-10177 JJ denotes accessory
T8160 10178-10182 NN denotes lobe
T8162 10183-10186 CC denotes and
T8163 10187-10188 DT denotes a
T8164 10189-10196 NN denotes portion
T8165 10197-10199 IN denotes of
T8166 10200-10203 DT denotes the
T8168 10204-10209 JJ denotes right
T8169 10210-10216 JJ denotes middle
T8167 10217-10221 NN denotes lobe
T8170 10221-10222 . denotes .
T8171 10222-10452 sentence denotes It is well established that abnormalities in either diaphragmatic development or fetal breathing can result in a secondary pulmonary hypoplasia, although loss of normal structure has never been documented in this context [17,18].
T8172 10223-10225 PRP denotes It
T8173 10226-10228 VBZ denotes is
T8174 10229-10233 RB denotes well
T8175 10234-10245 VBN denotes established
T8176 10246-10250 IN denotes that
T8178 10251-10264 NNS denotes abnormalities
T8179 10265-10267 IN denotes in
T8180 10268-10274 CC denotes either
T8182 10275-10288 JJ denotes diaphragmatic
T8181 10289-10300 NN denotes development
T8183 10301-10303 CC denotes or
T8184 10304-10309 JJ denotes fetal
T8185 10310-10319 NN denotes breathing
T8186 10320-10323 MD denotes can
T8177 10324-10330 VB denotes result
T8187 10331-10333 IN denotes in
T8188 10334-10335 DT denotes a
T8190 10336-10345 JJ denotes secondary
T8191 10346-10355 JJ denotes pulmonary
T8189 10356-10366 NN denotes hypoplasia
T8192 10366-10368 , denotes ,
T8193 10368-10376 IN denotes although
T8195 10377-10381 NN denotes loss
T8196 10382-10384 IN denotes of
T8197 10385-10391 JJ denotes normal
T8198 10392-10401 NN denotes structure
T8199 10402-10405 VBZ denotes has
T8200 10406-10411 RB denotes never
T8201 10412-10416 VBN denotes been
T8194 10417-10427 VBN denotes documented
T8202 10428-10430 IN denotes in
T8203 10431-10435 DT denotes this
T8204 10436-10443 NN denotes context
T8205 10444-10445 -LRB- denotes [
T8207 10445-10447 CD denotes 17
T8208 10447-10448 , denotes ,
T8206 10448-10450 CD denotes 18
T8209 10450-10451 -RRB- denotes ]
T8210 10451-10452 . denotes .
T8211 10452-10658 sentence denotes Fog2 is expressed diffusely in the pulmonary mesenchyme during the period of branching morphogenesis, while later expression is restricted to the smooth muscles of airways and pulmonary vessels (Figure 3).
T8212 10453-10457 NN denotes Fog2
T8214 10458-10460 VBZ denotes is
T8213 10461-10470 VBN denotes expressed
T8215 10471-10480 RB denotes diffusely
T8216 10481-10483 IN denotes in
T8217 10484-10487 DT denotes the
T8219 10488-10497 JJ denotes pulmonary
T8218 10498-10508 NN denotes mesenchyme
T8220 10509-10515 IN denotes during
T8221 10516-10519 DT denotes the
T8222 10520-10526 NN denotes period
T8223 10527-10529 IN denotes of
T8224 10530-10539 NN denotes branching
T8225 10540-10553 NN denotes morphogenesis
T8226 10553-10555 , denotes ,
T8227 10555-10560 IN denotes while
T8229 10561-10566 JJ denotes later
T8230 10567-10577 NN denotes expression
T8231 10578-10580 VBZ denotes is
T8228 10581-10591 VBN denotes restricted
T8232 10592-10594 IN denotes to
T8233 10595-10598 DT denotes the
T8235 10599-10605 JJ denotes smooth
T8234 10606-10613 NNS denotes muscles
T8236 10614-10616 IN denotes of
T8237 10617-10624 NNS denotes airways
T8238 10625-10628 CC denotes and
T8239 10629-10638 JJ denotes pulmonary
T8240 10639-10646 NNS denotes vessels
T8241 10647-10648 -LRB- denotes (
T8242 10648-10654 NN denotes Figure
T8243 10655-10656 CD denotes 3
T8244 10656-10657 -RRB- denotes )
T8245 10657-10658 . denotes .
T8246 10658-10884 sentence denotes This, and the observation that lungs appeared small on transverse sections evaluated prior to diaphragmatic muscularization or function, suggests that the pulmonary hypoplasia occurs independently of the diaphragmatic defect.
T8247 10659-10663 DT denotes This
T8249 10663-10665 , denotes ,
T8250 10665-10668 CC denotes and
T8251 10669-10672 DT denotes the
T8252 10673-10684 NN denotes observation
T8253 10685-10689 IN denotes that
T8255 10690-10695 NNS denotes lungs
T8254 10696-10704 VBD denotes appeared
T8256 10705-10710 JJ denotes small
T8257 10711-10713 IN denotes on
T8258 10714-10724 JJ denotes transverse
T8259 10725-10733 NNS denotes sections
T8260 10734-10743 VBN denotes evaluated
T8261 10744-10749 JJ denotes prior
T8262 10750-10752 IN denotes to
T8263 10753-10766 JJ denotes diaphragmatic
T8264 10767-10782 NN denotes muscularization
T8265 10783-10785 CC denotes or
T8266 10786-10794 NN denotes function
T8267 10794-10796 , denotes ,
T8248 10796-10804 VBZ denotes suggests
T8268 10805-10809 IN denotes that
T8270 10810-10813 DT denotes the
T8272 10814-10823 JJ denotes pulmonary
T8271 10824-10834 NN denotes hypoplasia
T8269 10835-10841 VBZ denotes occurs
T8273 10842-10855 RB denotes independently
T8274 10856-10858 IN denotes of
T8275 10859-10862 DT denotes the
T8277 10863-10876 JJ denotes diaphragmatic
T8276 10877-10883 NN denotes defect
T8278 10883-10884 . denotes .
T8279 10884-11021 sentence denotes To test this hypothesis, lungs were dissected from Fog2 −/− mice and littermate controls before the onset of fetal diaphragmatic motion.
T8280 10885-10887 TO denotes To
T8281 10888-10892 VB denotes test
T8283 10893-10897 DT denotes this
T8284 10898-10908 NN denotes hypothesis
T8285 10908-10910 , denotes ,
T8286 10910-10915 NNS denotes lungs
T8287 10916-10920 VBD denotes were
T8282 10921-10930 VBN denotes dissected
T8288 10931-10935 IN denotes from
T8289 10936-10940 NN denotes Fog2
T8291 10941-10942 SYM denotes −
T8292 10942-10943 HYPH denotes /
T8293 10943-10944 SYM denotes −
T8290 10945-10949 NNS denotes mice
T8294 10950-10953 CC denotes and
T8295 10954-10964 NN denotes littermate
T8296 10965-10973 NNS denotes controls
T8297 10974-10980 IN denotes before
T8298 10981-10984 DT denotes the
T8299 10985-10990 NN denotes onset
T8300 10991-10993 IN denotes of
T8301 10994-10999 JJ denotes fetal
T8303 11000-11013 JJ denotes diaphragmatic
T8302 11014-11020 NN denotes motion
T8304 11020-11021 . denotes .
T8305 11021-11153 sentence denotes Fog2 −/− mice were used for this experiment, as we wanted to avoid potential phenotypic variance from the lil hypomorphic mutation.
T8306 11022-11026 NN denotes Fog2
T8308 11027-11028 SYM denotes −
T8309 11028-11029 HYPH denotes /
T8310 11029-11030 SYM denotes −
T8307 11031-11035 NNS denotes mice
T8312 11036-11040 VBD denotes were
T8311 11041-11045 VBN denotes used
T8313 11046-11049 IN denotes for
T8314 11050-11054 DT denotes this
T8315 11055-11065 NN denotes experiment
T8316 11065-11067 , denotes ,
T8317 11067-11069 IN denotes as
T8319 11070-11072 PRP denotes we
T8318 11073-11079 VBD denotes wanted
T8320 11080-11082 TO denotes to
T8321 11083-11088 VB denotes avoid
T8322 11089-11098 JJ denotes potential
T8324 11099-11109 JJ denotes phenotypic
T8323 11110-11118 NN denotes variance
T8325 11119-11123 IN denotes from
T8326 11124-11127 DT denotes the
T8328 11128-11131 NN denotes lil
T8329 11132-11143 JJ denotes hypomorphic
T8327 11144-11152 NN denotes mutation
T8330 11152-11153 . denotes .
T8331 11153-11268 sentence denotes Lungs dissected at E12 from Fog2 −/− embryos were smaller in size and lacked the development of an accessory lobe.
T8332 11154-11159 NNS denotes Lungs
T8334 11160-11169 VBN denotes dissected
T8335 11170-11172 IN denotes at
T8336 11173-11176 NN denotes E12
T8337 11177-11181 IN denotes from
T8338 11182-11186 NN denotes Fog2
T8340 11187-11188 SYM denotes −
T8341 11188-11189 HYPH denotes /
T8342 11189-11190 SYM denotes −
T8339 11191-11198 NNS denotes embryos
T8333 11199-11203 VBD denotes were
T8343 11204-11211 JJR denotes smaller
T8344 11212-11214 IN denotes in
T8345 11215-11219 NN denotes size
T8346 11220-11223 CC denotes and
T8347 11224-11230 VBD denotes lacked
T8348 11231-11234 DT denotes the
T8349 11235-11246 NN denotes development
T8350 11247-11249 IN denotes of
T8351 11250-11252 DT denotes an
T8353 11253-11262 JJ denotes accessory
T8352 11263-11267 NN denotes lobe
T8354 11267-11268 . denotes .
T8355 11268-11483 sentence denotes In 11 viable Fog2− /− lung culture explants, there was never development of an accessory lobe, and the weights of mutant lungs cultured for 5 d were significantly lower than those of littermate controls (Figure 4).
T8356 11269-11271 IN denotes In
T8358 11272-11274 CD denotes 11
T8360 11275-11281 JJ denotes viable
T8361 11282-11286 NN denotes Fog2
T8362 11286-11287 SYM denotes −
T8363 11288-11289 HYPH denotes /
T8364 11289-11290 SYM denotes −
T8365 11291-11295 NN denotes lung
T8366 11296-11303 NN denotes culture
T8359 11304-11312 NNS denotes explants
T8367 11312-11314 , denotes ,
T8368 11314-11319 EX denotes there
T8357 11320-11323 VBD denotes was
T8369 11324-11329 RB denotes never
T8370 11330-11341 NN denotes development
T8371 11342-11344 IN denotes of
T8372 11345-11347 DT denotes an
T8374 11348-11357 JJ denotes accessory
T8373 11358-11362 NN denotes lobe
T8375 11362-11364 , denotes ,
T8376 11364-11367 CC denotes and
T8377 11368-11371 DT denotes the
T8378 11372-11379 NNS denotes weights
T8380 11380-11382 IN denotes of
T8381 11383-11389 NN denotes mutant
T8382 11390-11395 NNS denotes lungs
T8383 11396-11404 VBN denotes cultured
T8384 11405-11408 IN denotes for
T8385 11409-11410 CD denotes 5
T8386 11411-11412 NN denotes d
T8379 11413-11417 VBD denotes were
T8387 11418-11431 RB denotes significantly
T8388 11432-11437 JJR denotes lower
T8389 11438-11442 IN denotes than
T8390 11443-11448 DT denotes those
T8391 11449-11451 IN denotes of
T8392 11452-11462 NN denotes littermate
T8393 11463-11471 NNS denotes controls
T8394 11472-11473 -LRB- denotes (
T8395 11473-11479 NN denotes Figure
T8396 11480-11481 CD denotes 4
T8397 11481-11482 -RRB- denotes )
T8398 11482-11483 . denotes .
T8399 11483-11577 sentence denotes These data demonstrate that the pulmonary hypoplasia in Fog2 mutant mice is a primary defect.
T8400 11484-11489 DT denotes These
T8401 11490-11494 NNS denotes data
T8402 11495-11506 VBP denotes demonstrate
T8403 11507-11511 IN denotes that
T8405 11512-11515 DT denotes the
T8407 11516-11525 JJ denotes pulmonary
T8406 11526-11536 NN denotes hypoplasia
T8408 11537-11539 IN denotes in
T8409 11540-11544 NN denotes Fog2
T8411 11545-11551 NN denotes mutant
T8410 11552-11556 NNS denotes mice
T8404 11557-11559 VBZ denotes is
T8412 11560-11561 DT denotes a
T8414 11562-11569 JJ denotes primary
T8413 11570-11576 NN denotes defect
T8415 11576-11577 . denotes .
T8416 11577-12361 sentence denotes Figure 3 Fog2 Is Expressed in the Developing Lung and Diaphragm Fog2 is expressed in the diffuse pulmonary mesenchyme at E13.5 (A) (arrow shows mesenchyme) and is restricted to the bronchial and vascular smooth muscle (sm) at E16.5 (B). Fog2 is expressed diffusely in the developing diaphragm (Dia) both prior to (E11.5) (C) and after muscularization (E13.5) (D). Figure 4 Fog2 Is Necessary for Primary Lung Development Fog2 null lungs removed prior to diaphragmatic muscularization and grown in vitro show no accessory lobe development. Accessory lobe is labeled with black arrow in control littermate lungs. Branching in the unaffected lobes appeared to be delayed by 6–12 h, but all mutants developed an intricate branching pattern in the unaffected lobes after culture for 5 d.
T8417 12190-12199 NN denotes Branching
T8419 12200-12202 IN denotes in
T8420 12203-12206 DT denotes the
T8422 12207-12217 JJ denotes unaffected
T8421 12218-12223 NNS denotes lobes
T8418 12224-12232 VBD denotes appeared
T8423 12233-12235 TO denotes to
T8425 12236-12238 VB denotes be
T8424 12239-12246 VBN denotes delayed
T8426 12247-12249 IN denotes by
T8427 12250-12251 CD denotes 6
T8429 12251-12252 SYM denotes –
T8428 12252-12254 CD denotes 12
T8430 12255-12256 NN denotes h
T8431 12256-12258 , denotes ,
T8432 12258-12261 CC denotes but
T8433 12262-12265 DT denotes all
T8434 12266-12273 NNS denotes mutants
T8435 12274-12283 VBD denotes developed
T8436 12284-12286 DT denotes an
T8438 12287-12296 JJ denotes intricate
T8439 12297-12306 NN denotes branching
T8437 12307-12314 NN denotes pattern
T8440 12315-12317 IN denotes in
T8441 12318-12321 DT denotes the
T8443 12322-12332 JJ denotes unaffected
T8442 12333-12338 NNS denotes lobes
T8444 12339-12344 IN denotes after
T8445 12345-12352 NN denotes culture
T8446 12353-12356 IN denotes for
T8447 12357-12358 CD denotes 5
T8448 12359-12360 NN denotes d
T8449 12360-12361 . denotes .
T8450 12361-12627 sentence denotes Because the Fog2 phenotype is striking for specific lobar loss, the spatial pattern of Fog2 expression was evaluated in normal embryos during the period of early lobar establishment to determine whether Fog2 expression is specifically different at these lobar buds.
T8451 12362-12369 IN denotes Because
T8453 12370-12373 DT denotes the
T8455 12374-12378 NN denotes Fog2
T8454 12379-12388 NN denotes phenotype
T8452 12389-12391 VBZ denotes is
T8457 12392-12400 JJ denotes striking
T8458 12401-12404 IN denotes for
T8459 12405-12413 JJ denotes specific
T8461 12414-12419 NN denotes lobar
T8460 12420-12424 NN denotes loss
T8462 12424-12426 , denotes ,
T8463 12426-12429 DT denotes the
T8465 12430-12437 JJ denotes spatial
T8464 12438-12445 NN denotes pattern
T8466 12446-12448 IN denotes of
T8467 12449-12453 NN denotes Fog2
T8468 12454-12464 NN denotes expression
T8469 12465-12468 VBD denotes was
T8456 12469-12478 VBN denotes evaluated
T8470 12479-12481 IN denotes in
T8471 12482-12488 JJ denotes normal
T8472 12489-12496 NNS denotes embryos
T8473 12497-12503 IN denotes during
T8474 12504-12507 DT denotes the
T8475 12508-12514 NN denotes period
T8476 12515-12517 IN denotes of
T8477 12518-12523 JJ denotes early
T8479 12524-12529 NN denotes lobar
T8478 12530-12543 NN denotes establishment
T8480 12544-12546 TO denotes to
T8481 12547-12556 VB denotes determine
T8482 12557-12564 IN denotes whether
T8484 12565-12569 NN denotes Fog2
T8485 12570-12580 NN denotes expression
T8483 12581-12583 VBZ denotes is
T8486 12584-12596 RB denotes specifically
T8487 12597-12606 JJ denotes different
T8488 12607-12609 IN denotes at
T8489 12610-12615 DT denotes these
T8491 12616-12621 NN denotes lobar
T8490 12622-12626 NNS denotes buds
T8492 12626-12627 . denotes .
T8493 12627-12747 sentence denotes Expression was evaluated in mice carrying a lacZ gene incorporated into the Fog2 locus (S. Tevosian, unpublished data).
T8494 12628-12638 NN denotes Expression
T8496 12639-12642 VBD denotes was
T8495 12643-12652 VBN denotes evaluated
T8497 12653-12655 IN denotes in
T8498 12656-12660 NNS denotes mice
T8499 12661-12669 VBG denotes carrying
T8500 12670-12671 DT denotes a
T8502 12672-12676 NN denotes lacZ
T8501 12677-12681 NN denotes gene
T8503 12682-12694 VBN denotes incorporated
T8504 12695-12699 IN denotes into
T8505 12700-12703 DT denotes the
T8507 12704-12708 NN denotes Fog2
T8506 12709-12714 NN denotes locus
T8508 12715-12716 -LRB- denotes (
T8509 12716-12718 NNP denotes S.
T8510 12719-12727 NNP denotes Tevosian
T8511 12727-12729 , denotes ,
T8512 12729-12740 JJ denotes unpublished
T8513 12741-12745 NNS denotes data
T8514 12745-12746 -RRB- denotes )
T8515 12746-12747 . denotes .
T8516 12747-12998 sentence denotes In nine mice examined at E11.5, all lungs showed a specific enhancement of Fog2 expression in the mesenchyme surrounding the accessory bud and the right middle lobe bud, which are the lobes that do not develop normally in Fog2 mutant mice (Figure 5).
T8517 12748-12750 IN denotes In
T8519 12751-12755 CD denotes nine
T8520 12756-12760 NNS denotes mice
T8521 12761-12769 VBN denotes examined
T8522 12770-12772 IN denotes at
T8523 12773-12778 NN denotes E11.5
T8524 12778-12780 , denotes ,
T8525 12780-12783 DT denotes all
T8526 12784-12789 NNS denotes lungs
T8518 12790-12796 VBD denotes showed
T8527 12797-12798 DT denotes a
T8529 12799-12807 JJ denotes specific
T8528 12808-12819 NN denotes enhancement
T8530 12820-12822 IN denotes of
T8531 12823-12827 NN denotes Fog2
T8532 12828-12838 NN denotes expression
T8533 12839-12841 IN denotes in
T8534 12842-12845 DT denotes the
T8535 12846-12856 NN denotes mesenchyme
T8536 12857-12868 VBG denotes surrounding
T8537 12869-12872 DT denotes the
T8539 12873-12882 JJ denotes accessory
T8538 12883-12886 NN denotes bud
T8540 12887-12890 CC denotes and
T8541 12891-12894 DT denotes the
T8543 12895-12900 JJ denotes right
T8544 12901-12907 JJ denotes middle
T8545 12908-12912 NN denotes lobe
T8542 12913-12916 NN denotes bud
T8546 12916-12918 , denotes ,
T8547 12918-12923 WDT denotes which
T8548 12924-12927 VBP denotes are
T8549 12928-12931 DT denotes the
T8550 12932-12937 NNS denotes lobes
T8551 12938-12942 WDT denotes that
T8553 12943-12945 VBP denotes do
T8554 12946-12949 RB denotes not
T8552 12950-12957 VB denotes develop
T8555 12958-12966 RB denotes normally
T8556 12967-12969 IN denotes in
T8557 12970-12974 NN denotes Fog2
T8559 12975-12981 NN denotes mutant
T8558 12982-12986 NNS denotes mice
T8560 12987-12988 -LRB- denotes (
T8561 12988-12994 NN denotes Figure
T8562 12995-12996 CD denotes 5
T8563 12996-12997 -RRB- denotes )
T8564 12997-12998 . denotes .
T8565 12998-13130 sentence denotes By E12.5, expression was diffuse in the pulmonary mesenchyme, as was seen previously with in situ hybridization on tissue sections.
T8566 12999-13001 IN denotes By
T8568 13002-13007 NN denotes E12.5
T8569 13007-13009 , denotes ,
T8570 13009-13019 NN denotes expression
T8567 13020-13023 VBD denotes was
T8571 13024-13031 JJ denotes diffuse
T8572 13032-13034 IN denotes in
T8573 13035-13038 DT denotes the
T8575 13039-13048 JJ denotes pulmonary
T8574 13049-13059 NN denotes mesenchyme
T8576 13059-13061 , denotes ,
T8577 13061-13063 IN denotes as
T8579 13064-13067 VBD denotes was
T8578 13068-13072 VBN denotes seen
T8580 13073-13083 RB denotes previously
T8581 13084-13088 IN denotes with
T8582 13089-13091 FW denotes in
T8583 13092-13096 FW denotes situ
T8584 13097-13110 NN denotes hybridization
T8585 13111-13113 IN denotes on
T8586 13114-13120 NN denotes tissue
T8587 13121-13129 NNS denotes sections
T8588 13129-13130 . denotes .
T8589 13130-13512 sentence denotes Figure 5 Fog2 Expression in Embryonic Non-Mutant Lungs In embryonic non-mutant lungs, Fog2 is most highly expressed at the tips of the accessory (single arrows) and right middle lobes (double arrows) at E11.25 (A) and E11.75 (B), while expression is diffuse by E12.75 (C). Diaphragms from Fog2 lil mice show an intact membrane with a defect in muscular patterning (see Figure 1B).
T8590 13405-13415 NNS denotes Diaphragms
T8592 13416-13420 IN denotes from
T8593 13421-13425 NN denotes Fog2
T8595 13426-13429 NN denotes lil
T8594 13430-13434 NNS denotes mice
T8591 13435-13439 VBP denotes show
T8596 13440-13442 DT denotes an
T8598 13443-13449 JJ denotes intact
T8597 13450-13458 NN denotes membrane
T8599 13459-13463 IN denotes with
T8600 13464-13465 DT denotes a
T8601 13466-13472 NN denotes defect
T8602 13473-13475 IN denotes in
T8603 13476-13484 JJ denotes muscular
T8604 13485-13495 NN denotes patterning
T8605 13496-13497 -LRB- denotes (
T8606 13497-13500 VB denotes see
T8607 13501-13507 NN denotes Figure
T8608 13508-13510 CD denotes 1B
T8609 13510-13511 -RRB- denotes )
T8610 13511-13512 . denotes .
T8611 13512-13647 sentence denotes The membranous portion of the diaphragm is populated by a migratory population of muscle precursor cells, much like the limbs [19,20].
T8612 13513-13516 DT denotes The
T8614 13517-13527 JJ denotes membranous
T8613 13528-13535 NN denotes portion
T8616 13536-13538 IN denotes of
T8617 13539-13542 DT denotes the
T8618 13543-13552 NN denotes diaphragm
T8619 13553-13555 VBZ denotes is
T8615 13556-13565 VBN denotes populated
T8620 13566-13568 IN denotes by
T8621 13569-13570 DT denotes a
T8623 13571-13580 JJ denotes migratory
T8622 13581-13591 NN denotes population
T8624 13592-13594 IN denotes of
T8625 13595-13601 NN denotes muscle
T8627 13602-13611 NN denotes precursor
T8626 13612-13617 NNS denotes cells
T8628 13617-13619 , denotes ,
T8629 13619-13623 RB denotes much
T8630 13624-13628 IN denotes like
T8631 13629-13632 DT denotes the
T8632 13633-13638 NNS denotes limbs
T8633 13639-13640 -LRB- denotes [
T8635 13640-13642 CD denotes 19
T8636 13642-13643 , denotes ,
T8634 13643-13645 CD denotes 20
T8637 13645-13646 -RRB- denotes ]
T8638 13646-13647 . denotes .
T8639 13647-13777 sentence denotes Mice with defects in genes known to be important for the control of this process have intact but amuscular diaphragms [17,21,22].
T8640 13648-13652 NNS denotes Mice
T8642 13653-13657 IN denotes with
T8643 13658-13665 NNS denotes defects
T8644 13666-13668 IN denotes in
T8645 13669-13674 NNS denotes genes
T8646 13675-13680 VBN denotes known
T8647 13681-13683 TO denotes to
T8648 13684-13686 VB denotes be
T8649 13687-13696 JJ denotes important
T8650 13697-13700 IN denotes for
T8651 13701-13704 DT denotes the
T8652 13705-13712 NN denotes control
T8653 13713-13715 IN denotes of
T8654 13716-13720 DT denotes this
T8655 13721-13728 NN denotes process
T8641 13729-13733 VBP denotes have
T8656 13734-13740 JJ denotes intact
T8658 13741-13744 CC denotes but
T8659 13745-13754 JJ denotes amuscular
T8657 13755-13765 NNS denotes diaphragms
T8660 13766-13767 -LRB- denotes [
T8662 13767-13769 CD denotes 17
T8663 13769-13770 , denotes ,
T8664 13770-13772 CD denotes 21
T8665 13772-13773 , denotes ,
T8661 13773-13775 CD denotes 22
T8666 13775-13776 -RRB- denotes ]
T8667 13776-13777 . denotes .
T8668 13777-13929 sentence denotes Hepatocyte growth factor/Scatter factor (HGF) is one potential candidate responsible for the guidance of muscle precursors to the membranous diaphragm.
T8669 13778-13788 NN denotes Hepatocyte
T8671 13789-13795 NN denotes growth
T8670 13796-13802 NN denotes factor
T8673 13802-13803 HYPH denotes /
T8674 13803-13810 NN denotes Scatter
T8675 13811-13817 NN denotes factor
T8676 13818-13819 -LRB- denotes (
T8677 13819-13822 NN denotes HGF
T8678 13822-13823 -RRB- denotes )
T8672 13824-13826 VBZ denotes is
T8679 13827-13830 CD denotes one
T8681 13831-13840 JJ denotes potential
T8680 13841-13850 NN denotes candidate
T8682 13851-13862 JJ denotes responsible
T8683 13863-13866 IN denotes for
T8684 13867-13870 DT denotes the
T8685 13871-13879 NN denotes guidance
T8686 13880-13882 IN denotes of
T8687 13883-13889 NN denotes muscle
T8688 13890-13900 NNS denotes precursors
T8689 13901-13903 IN denotes to
T8690 13904-13907 DT denotes the
T8692 13908-13918 JJ denotes membranous
T8691 13919-13928 NN denotes diaphragm
T8693 13928-13929 . denotes .
T8694 13929-14152 sentence denotes It has been shown that HGF is expressed along this anatomic pathway [23], and mice with absence of the HGF receptor c-Met fail to form muscularized pleuroperitoneal folds (PPFs), and thus have amuscular diaphragms [24,25].
T8695 13930-13932 PRP denotes It
T8697 13933-13936 VBZ denotes has
T8698 13937-13941 VBN denotes been
T8696 13942-13947 VBN denotes shown
T8699 13948-13952 IN denotes that
T8701 13953-13956 NN denotes HGF
T8702 13957-13959 VBZ denotes is
T8700 13960-13969 VBN denotes expressed
T8703 13970-13975 IN denotes along
T8704 13976-13980 DT denotes this
T8706 13981-13989 JJ denotes anatomic
T8705 13990-13997 NN denotes pathway
T8707 13998-13999 -LRB- denotes [
T8708 13999-14001 CD denotes 23
T8709 14001-14002 -RRB- denotes ]
T8710 14002-14004 , denotes ,
T8711 14004-14007 CC denotes and
T8712 14008-14012 NNS denotes mice
T8714 14013-14017 IN denotes with
T8715 14018-14025 NN denotes absence
T8716 14026-14028 IN denotes of
T8717 14029-14032 DT denotes the
T8719 14033-14036 NN denotes HGF
T8718 14037-14045 NN denotes receptor
T8720 14046-14047 NN denotes c
T8722 14047-14048 HYPH denotes -
T8721 14048-14051 NN denotes Met
T8713 14052-14056 VBP denotes fail
T8723 14057-14059 TO denotes to
T8724 14060-14064 VB denotes form
T8725 14065-14077 VBN denotes muscularized
T8727 14078-14094 JJ denotes pleuroperitoneal
T8726 14095-14100 NNS denotes folds
T8728 14101-14102 -LRB- denotes (
T8729 14102-14106 NNS denotes PPFs
T8730 14106-14107 -RRB- denotes )
T8731 14107-14109 , denotes ,
T8732 14109-14112 CC denotes and
T8733 14113-14117 RB denotes thus
T8734 14118-14122 VBP denotes have
T8735 14123-14132 JJ denotes amuscular
T8736 14133-14143 NNS denotes diaphragms
T8737 14144-14145 -LRB- denotes [
T8739 14145-14147 CD denotes 24
T8740 14147-14148 , denotes ,
T8738 14148-14150 CD denotes 25
T8741 14150-14151 -RRB- denotes ]
T8742 14151-14152 . denotes .
T8743 14152-14293 sentence denotes Fog2 is expressed diffusely in the early amuscular diaphragm at E11.5 as well as in the later muscularized diaphragm (see Figure 3C and 3D).
T8744 14153-14157 NN denotes Fog2
T8746 14158-14160 VBZ denotes is
T8745 14161-14170 VBN denotes expressed
T8747 14171-14180 RB denotes diffusely
T8748 14181-14183 IN denotes in
T8749 14184-14187 DT denotes the
T8751 14188-14193 JJ denotes early
T8752 14194-14203 JJ denotes amuscular
T8750 14204-14213 NN denotes diaphragm
T8753 14214-14216 IN denotes at
T8754 14217-14222 NN denotes E11.5
T8755 14223-14225 RB denotes as
T8757 14226-14230 RB denotes well
T8756 14231-14233 IN denotes as
T8758 14234-14236 IN denotes in
T8759 14237-14240 DT denotes the
T8761 14241-14246 JJ denotes later
T8762 14247-14259 VBN denotes muscularized
T8760 14260-14269 NN denotes diaphragm
T8763 14270-14271 -LRB- denotes (
T8764 14271-14274 VB denotes see
T8765 14275-14281 NN denotes Figure
T8766 14282-14284 CD denotes 3C
T8767 14285-14288 CC denotes and
T8768 14289-14291 CD denotes 3D
T8769 14291-14292 -RRB- denotes )
T8770 14292-14293 . denotes .
T8771 14293-14468 sentence denotes Pax3 and MyoD, transcription factors required for appropriate migration and determination of myogenic precursors, were detected in the PPFs of Fog2 lil mice (data not shown).
T8772 14294-14298 NN denotes Pax3
T8774 14299-14302 CC denotes and
T8775 14303-14307 NN denotes MyoD
T8776 14307-14309 , denotes ,
T8777 14309-14322 NN denotes transcription
T8778 14323-14330 NNS denotes factors
T8779 14331-14339 VBN denotes required
T8780 14340-14343 IN denotes for
T8781 14344-14355 JJ denotes appropriate
T8782 14356-14365 NN denotes migration
T8783 14366-14369 CC denotes and
T8784 14370-14383 NN denotes determination
T8785 14384-14386 IN denotes of
T8786 14387-14395 JJ denotes myogenic
T8787 14396-14406 NNS denotes precursors
T8788 14406-14408 , denotes ,
T8789 14408-14412 VBD denotes were
T8773 14413-14421 VBN denotes detected
T8790 14422-14424 IN denotes in
T8791 14425-14428 DT denotes the
T8792 14429-14433 NNS denotes PPFs
T8793 14434-14436 IN denotes of
T8794 14437-14441 NN denotes Fog2
T8796 14442-14445 NN denotes lil
T8795 14446-14450 NNS denotes mice
T8797 14451-14452 -LRB- denotes (
T8799 14452-14456 NNS denotes data
T8800 14457-14460 RB denotes not
T8798 14461-14466 VBN denotes shown
T8801 14466-14467 -RRB- denotes )
T8802 14467-14468 . denotes .
T8803 14468-14664 sentence denotes However, in situ expression analysis demonstrated that the expression of HGF in the region where this structure meets the membranous diaphragm was markedly reduced in Fog2 mutant mice (Figure 6).
T8804 14469-14476 RB denotes However
T8806 14476-14478 , denotes ,
T8807 14478-14480 FW denotes in
T8808 14481-14485 FW denotes situ
T8810 14486-14496 NN denotes expression
T8809 14497-14505 NN denotes analysis
T8805 14506-14518 VBD denotes demonstrated
T8811 14519-14523 IN denotes that
T8813 14524-14527 DT denotes the
T8814 14528-14538 NN denotes expression
T8815 14539-14541 IN denotes of
T8816 14542-14545 NN denotes HGF
T8817 14546-14548 IN denotes in
T8818 14549-14552 DT denotes the
T8819 14553-14559 NN denotes region
T8820 14560-14565 WRB denotes where
T8822 14566-14570 DT denotes this
T8823 14571-14580 NN denotes structure
T8821 14581-14586 VBZ denotes meets
T8824 14587-14590 DT denotes the
T8826 14591-14601 JJ denotes membranous
T8825 14602-14611 NN denotes diaphragm
T8827 14612-14615 VBD denotes was
T8828 14616-14624 RB denotes markedly
T8812 14625-14632 VBN denotes reduced
T8829 14633-14635 IN denotes in
T8830 14636-14640 NN denotes Fog2
T8832 14641-14647 NN denotes mutant
T8831 14648-14652 NNS denotes mice
T8833 14653-14654 -LRB- denotes (
T8834 14654-14660 NN denotes Figure
T8835 14661-14662 CD denotes 6
T8836 14662-14663 -RRB- denotes )
T8837 14663-14664 . denotes .
T8838 14664-14958 sentence denotes We hypothesize that Fog2 is required (either directly or indirectly) for the induction of HGF in the developing diaphragm, and dysregulation of HGF patterning along the path of muscle precursor cell migration between the PPF and the diaphragm accounts for the abnormal phenotype in these mice.
T8839 14665-14667 PRP denotes We
T8840 14668-14679 VBP denotes hypothesize
T8841 14680-14684 IN denotes that
T8843 14685-14689 NN denotes Fog2
T8844 14690-14692 VBZ denotes is
T8842 14693-14701 VBN denotes required
T8845 14702-14703 -LRB- denotes (
T8846 14703-14709 CC denotes either
T8847 14710-14718 RB denotes directly
T8848 14719-14721 CC denotes or
T8849 14722-14732 RB denotes indirectly
T8850 14732-14733 -RRB- denotes )
T8851 14734-14737 IN denotes for
T8852 14738-14741 DT denotes the
T8853 14742-14751 NN denotes induction
T8854 14752-14754 IN denotes of
T8855 14755-14758 NN denotes HGF
T8856 14759-14761 IN denotes in
T8857 14762-14765 DT denotes the
T8859 14766-14776 VBG denotes developing
T8858 14777-14786 NN denotes diaphragm
T8860 14786-14788 , denotes ,
T8861 14788-14791 CC denotes and
T8862 14792-14805 NN denotes dysregulation
T8864 14806-14808 IN denotes of
T8865 14809-14812 NN denotes HGF
T8866 14813-14823 NN denotes patterning
T8867 14824-14829 IN denotes along
T8868 14830-14833 DT denotes the
T8869 14834-14838 NN denotes path
T8870 14839-14841 IN denotes of
T8871 14842-14848 NN denotes muscle
T8873 14849-14858 NN denotes precursor
T8872 14859-14863 NN denotes cell
T8874 14864-14873 NN denotes migration
T8875 14874-14881 IN denotes between
T8876 14882-14885 DT denotes the
T8877 14886-14889 NN denotes PPF
T8878 14890-14893 CC denotes and
T8879 14894-14897 DT denotes the
T8880 14898-14907 NN denotes diaphragm
T8863 14908-14916 VBZ denotes accounts
T8881 14917-14920 IN denotes for
T8882 14921-14924 DT denotes the
T8884 14925-14933 JJ denotes abnormal
T8883 14934-14943 NN denotes phenotype
T8885 14944-14946 IN denotes in
T8886 14947-14952 DT denotes these
T8887 14953-14957 NNS denotes mice
T8888 14957-14958 . denotes .
T9907 15207-15211 NN denotes FOG2
T9908 15212-15220 NN denotes Mutation
T9909 15221-15223 IN denotes in
T9910 15224-15225 DT denotes a
T9911 15226-15233 NN denotes Patient
T9912 15234-15238 IN denotes with
T9913 15239-15248 NN denotes Diaphragm
T9915 15249-15252 CC denotes and
T9916 15253-15257 NN denotes Lung
T9914 15258-15271 NNS denotes Abnormalities
T9917 15271-15495 sentence denotes FOG2 sequence analysis was performed on autopsy tissue from 30 of 32 deceased children with an anatomic diagnosis of diaphragmatic defect evaluated at the Children's Hospital in Boston, Massachusetts, between 1993 and 2003.
T9918 15272-15276 NN denotes FOG2
T9920 15277-15285 NN denotes sequence
T9919 15286-15294 NN denotes analysis
T9922 15295-15298 VBD denotes was
T9921 15299-15308 VBN denotes performed
T9923 15309-15311 IN denotes on
T9924 15312-15319 NN denotes autopsy
T9925 15320-15326 NN denotes tissue
T9926 15327-15331 IN denotes from
T9927 15332-15334 CD denotes 30
T9929 15335-15337 IN denotes of
T9928 15338-15340 CD denotes 32
T9931 15341-15349 VBN denotes deceased
T9930 15350-15358 NNS denotes children
T9932 15359-15363 IN denotes with
T9933 15364-15366 DT denotes an
T9935 15367-15375 JJ denotes anatomic
T9934 15376-15385 NN denotes diagnosis
T9936 15386-15388 IN denotes of
T9937 15389-15402 JJ denotes diaphragmatic
T9938 15403-15409 NN denotes defect
T9939 15410-15419 VBN denotes evaluated
T9940 15420-15422 IN denotes at
T9941 15423-15426 DT denotes the
T9943 15427-15435 NNPS denotes Children
T9944 15435-15437 POS denotes 's
T9942 15438-15446 NNP denotes Hospital
T9945 15447-15449 IN denotes in
T9946 15450-15456 NNP denotes Boston
T9947 15456-15458 , denotes ,
T9948 15458-15471 NNP denotes Massachusetts
T9949 15471-15473 , denotes ,
T9950 15473-15480 IN denotes between
T9951 15481-15485 CD denotes 1993
T9952 15486-15489 CC denotes and
T9953 15490-15494 CD denotes 2003
T9954 15494-15495 . denotes .
T9955 15495-15562 sentence denotes Autopsy reports were reviewed to determine the specific diagnoses.
T9956 15496-15503 NN denotes Autopsy
T9957 15504-15511 NNS denotes reports
T9959 15512-15516 VBD denotes were
T9958 15517-15525 VBN denotes reviewed
T9960 15526-15528 TO denotes to
T9961 15529-15538 VB denotes determine
T9962 15539-15542 DT denotes the
T9964 15543-15551 JJ denotes specific
T9963 15552-15561 NNS denotes diagnoses
T9965 15561-15562 . denotes .
T9966 15562-15824 sentence denotes Of these 30 cases, 17 (57%) had Bochdalek CDH, two (7%) had diaphragmatic agenesis, seven (23%) had diaphragmatic eventration/muscularization defects (without Bochdalek CDH), and four (13%) had Bochdalek hernia of one hemidiaphragm and eventration of the other.
T9967 15563-15565 IN denotes Of
T9969 15566-15571 DT denotes these
T9971 15572-15574 CD denotes 30
T9970 15575-15580 NNS denotes cases
T9972 15580-15582 , denotes ,
T9973 15582-15584 CD denotes 17
T9974 15585-15586 -LRB- denotes (
T9976 15586-15588 CD denotes 57
T9975 15588-15589 NN denotes %
T9977 15589-15590 -RRB- denotes )
T9968 15591-15594 VBD denotes had
T9978 15595-15604 NNP denotes Bochdalek
T9979 15605-15608 NN denotes CDH
T9980 15608-15610 , denotes ,
T9981 15610-15613 CD denotes two
T9983 15614-15615 -LRB- denotes (
T9985 15615-15616 CD denotes 7
T9984 15616-15617 NN denotes %
T9986 15617-15618 -RRB- denotes )
T9982 15619-15622 VBD denotes had
T9987 15623-15636 JJ denotes diaphragmatic
T9988 15637-15645 NN denotes agenesis
T9989 15645-15647 , denotes ,
T9990 15647-15652 CD denotes seven
T9992 15653-15654 -LRB- denotes (
T9994 15654-15656 CD denotes 23
T9993 15656-15657 NN denotes %
T9995 15657-15658 -RRB- denotes )
T9991 15659-15662 VBD denotes had
T9996 15663-15676 JJ denotes diaphragmatic
T9997 15677-15688 NN denotes eventration
T9998 15688-15689 HYPH denotes /
T9999 15689-15704 NN denotes muscularization
T10000 15705-15712 NNS denotes defects
T10001 15713-15714 -LRB- denotes (
T10002 15714-15721 IN denotes without
T10003 15722-15731 NNP denotes Bochdalek
T10004 15732-15735 NN denotes CDH
T10005 15735-15736 -RRB- denotes )
T10006 15736-15738 , denotes ,
T10007 15738-15741 CC denotes and
T10008 15742-15746 CD denotes four
T10010 15747-15748 -LRB- denotes (
T10012 15748-15750 CD denotes 13
T10011 15750-15751 NN denotes %
T10013 15751-15752 -RRB- denotes )
T10009 15753-15756 VBD denotes had
T10014 15757-15766 NNP denotes Bochdalek
T10015 15767-15773 NN denotes hernia
T10016 15774-15776 IN denotes of
T10017 15777-15780 CD denotes one
T10018 15781-15794 NN denotes hemidiaphragm
T10019 15795-15798 CC denotes and
T10020 15799-15810 NN denotes eventration
T10021 15811-15813 IN denotes of
T10022 15814-15817 DT denotes the
T10023 15818-15823 NN denotes other
T10024 15823-15824 . denotes .
T10025 15824-15920 sentence denotes Pulmonary hypoplasia was assessed using lung/body weight ratio and radial alveolar counts [26].
T10026 15825-15834 JJ denotes Pulmonary
T10027 15835-15845 NN denotes hypoplasia
T10029 15846-15849 VBD denotes was
T10028 15850-15858 VBN denotes assessed
T10030 15859-15864 VBG denotes using
T10031 15865-15869 NN denotes lung
T10033 15869-15870 HYPH denotes /
T10032 15870-15874 NN denotes body
T10035 15875-15881 NN denotes weight
T10034 15882-15887 NN denotes ratio
T10036 15888-15891 CC denotes and
T10037 15892-15898 JJ denotes radial
T10039 15899-15907 JJ denotes alveolar
T10038 15908-15914 NNS denotes counts
T10040 15915-15916 -LRB- denotes [
T10041 15916-15918 CD denotes 26
T10042 15918-15919 -RRB- denotes ]
T10043 15919-15920 . denotes .
T10044 15920-16058 sentence denotes The material available for review included written reports and histologic slides in all cases and gross kodachromes in a subset of cases.
T10045 15921-15924 DT denotes The
T10046 15925-15933 NN denotes material
T10048 15934-15943 JJ denotes available
T10049 15944-15947 IN denotes for
T10050 15948-15954 NN denotes review
T10047 15955-15963 VBN denotes included
T10051 15964-15971 VBN denotes written
T10052 15972-15979 NNS denotes reports
T10053 15980-15983 CC denotes and
T10054 15984-15994 JJ denotes histologic
T10055 15995-16001 NNS denotes slides
T10056 16002-16004 IN denotes in
T10057 16005-16008 DT denotes all
T10058 16009-16014 NNS denotes cases
T10059 16015-16018 CC denotes and
T10060 16019-16024 JJ denotes gross
T10061 16025-16036 NNS denotes kodachromes
T10062 16037-16039 IN denotes in
T10063 16040-16041 DT denotes a
T10064 16042-16048 NN denotes subset
T10065 16049-16051 IN denotes of
T10066 16052-16057 NNS denotes cases
T10067 16057-16058 . denotes .
T10068 16058-16119 sentence denotes One child carried a highly significant FOG2 sequence change.
T10069 16059-16062 CD denotes One
T10070 16063-16068 NN denotes child
T10071 16069-16076 VBD denotes carried
T10072 16077-16078 DT denotes a
T10074 16079-16085 RB denotes highly
T10075 16086-16097 JJ denotes significant
T10076 16098-16102 NN denotes FOG2
T10077 16103-16111 NN denotes sequence
T10073 16112-16118 NN denotes change
T10078 16118-16119 . denotes .
T10079 16119-16252 sentence denotes The patient was a full-term 3,500-g baby girl who developed severe respiratory failure at birth and died after 5 h of resuscitation.
T10080 16120-16123 DT denotes The
T10081 16124-16131 NN denotes patient
T10082 16132-16135 VBD denotes was
T10083 16136-16137 DT denotes a
T10085 16138-16142 JJ denotes full
T10087 16142-16143 HYPH denotes -
T10086 16143-16147 NN denotes term
T10088 16148-16153 CD denotes 3,500
T10090 16153-16154 HYPH denotes -
T10089 16154-16155 NN denotes g
T10091 16156-16160 NN denotes baby
T10084 16161-16165 NN denotes girl
T10092 16166-16169 WP denotes who
T10093 16170-16179 VBD denotes developed
T10094 16180-16186 JJ denotes severe
T10096 16187-16198 JJ denotes respiratory
T10095 16199-16206 NN denotes failure
T10097 16207-16209 IN denotes at
T10098 16210-16215 NN denotes birth
T10099 16216-16219 CC denotes and
T10100 16220-16224 VBD denotes died
T10101 16225-16230 IN denotes after
T10102 16231-16232 CD denotes 5
T10103 16233-16234 NN denotes h
T10104 16235-16237 IN denotes of
T10105 16238-16251 NN denotes resuscitation
T10106 16251-16252 . denotes .
T10107 16252-16337 sentence denotes Antemortem radiographs showed opacified lung fields and possible bowel in the chest.
T10108 16253-16263 NN denotes Antemortem
T10109 16264-16275 NNS denotes radiographs
T10110 16276-16282 VBD denotes showed
T10111 16283-16292 VBN denotes opacified
T10113 16293-16297 NN denotes lung
T10112 16298-16304 NNS denotes fields
T10114 16305-16308 CC denotes and
T10115 16309-16317 JJ denotes possible
T10116 16318-16323 NN denotes bowel
T10117 16324-16326 IN denotes in
T10118 16327-16330 DT denotes the
T10119 16331-16336 NN denotes chest
T10120 16336-16337 . denotes .
T10121 16337-16379 sentence denotes The patient's clinical diagnosis was CDH.
T10122 16338-16341 DT denotes The
T10123 16342-16349 NN denotes patient
T10125 16349-16351 POS denotes 's
T10126 16352-16360 JJ denotes clinical
T10124 16361-16370 NN denotes diagnosis
T10127 16371-16374 VBD denotes was
T10128 16375-16378 NN denotes CDH
T10129 16378-16379 . denotes .
T10130 16379-16578 sentence denotes Review of autopsy material revealed severe bilateral pulmonary hypoplasia (combined lung weight, 11.1 g; expected for body length/weight, 46.8 ± 26.2 g; [27]), most markedly involving the left lung.
T10131 16380-16386 NN denotes Review
T10133 16387-16389 IN denotes of
T10134 16390-16397 NN denotes autopsy
T10135 16398-16406 NN denotes material
T10132 16407-16415 VBD denotes revealed
T10136 16416-16422 JJ denotes severe
T10138 16423-16432 JJ denotes bilateral
T10139 16433-16442 JJ denotes pulmonary
T10137 16443-16453 NN denotes hypoplasia
T10140 16454-16455 -LRB- denotes (
T10142 16455-16463 VBN denotes combined
T10144 16464-16468 NN denotes lung
T10143 16469-16475 NN denotes weight
T10146 16475-16477 , denotes ,
T10147 16477-16481 CD denotes 11.1
T10145 16482-16483 NN denotes g
T10148 16483-16484 : denotes ;
T10149 16485-16493 VBN denotes expected
T10150 16494-16497 IN denotes for
T10151 16498-16502 NN denotes body
T10153 16503-16509 NN denotes length
T10154 16509-16510 HYPH denotes /
T10152 16510-16516 NN denotes weight
T10155 16516-16518 , denotes ,
T10156 16518-16522 CD denotes 46.8
T10158 16523-16524 SYM denotes ±
T10157 16525-16529 CD denotes 26.2
T10159 16530-16531 NN denotes g
T10160 16531-16532 : denotes ;
T10161 16533-16534 -LRB- denotes [
T10141 16534-16536 CD denotes 27
T10162 16536-16537 -RRB- denotes ]
T10163 16537-16538 -RRB- denotes )
T10164 16538-16540 , denotes ,
T10165 16540-16544 RBS denotes most
T10166 16545-16553 RB denotes markedly
T10167 16554-16563 VBG denotes involving
T10168 16564-16567 DT denotes the
T10170 16568-16572 JJ denotes left
T10169 16573-16577 NN denotes lung
T10171 16577-16578 . denotes .
T10172 16578-16641 sentence denotes The lung/body weight ratio was 0.0037 (expected > 0.010) [28].
T10173 16579-16582 DT denotes The
T10175 16583-16587 NN denotes lung
T10176 16587-16588 SYM denotes /
T10177 16588-16592 NN denotes body
T10178 16593-16599 NN denotes weight
T10174 16600-16605 NN denotes ratio
T10179 16606-16609 VBD denotes was
T10180 16610-16616 CD denotes 0.0037
T10181 16617-16618 -LRB- denotes (
T10183 16618-16626 VBN denotes expected
T10184 16627-16628 SYM denotes >
T10182 16629-16634 CD denotes 0.010
T10185 16634-16635 -RRB- denotes )
T10186 16636-16637 -LRB- denotes [
T10187 16637-16639 CD denotes 28
T10188 16639-16640 -RRB- denotes ]
T10189 16640-16641 . denotes .
T10190 16641-16754 sentence denotes There were a reduced number of bronchial generations, and the radial alveolar count was 2–3 (expected = 5) [29].
T10191 16642-16647 EX denotes There
T10192 16648-16652 VBD denotes were
T10193 16653-16654 DT denotes a
T10195 16655-16662 VBN denotes reduced
T10194 16663-16669 NN denotes number
T10196 16670-16672 IN denotes of
T10197 16673-16682 JJ denotes bronchial
T10198 16683-16694 NNS denotes generations
T10199 16694-16696 , denotes ,
T10200 16696-16699 CC denotes and
T10201 16700-16703 DT denotes the
T10203 16704-16710 JJ denotes radial
T10204 16711-16719 JJ denotes alveolar
T10202 16720-16725 NN denotes count
T10205 16726-16729 VBD denotes was
T10206 16730-16731 CD denotes 2
T10208 16731-16732 SYM denotes –
T10207 16732-16733 CD denotes 3
T10209 16734-16735 -LRB- denotes (
T10211 16735-16743 VBN denotes expected
T10212 16744-16745 SYM denotes =
T10210 16746-16747 CD denotes 5
T10213 16747-16748 -RRB- denotes )
T10214 16749-16750 -LRB- denotes [
T10215 16750-16752 CD denotes 29
T10216 16752-16753 -RRB- denotes ]
T10217 16753-16754 . denotes .
T10218 16754-16803 sentence denotes There were incomplete lung fissures bilaterally.
T10219 16755-16760 EX denotes There
T10220 16761-16765 VBD denotes were
T10221 16766-16776 JJ denotes incomplete
T10223 16777-16781 NN denotes lung
T10222 16782-16790 NNS denotes fissures
T10224 16791-16802 RB denotes bilaterally
T10225 16802-16803 . denotes .
T10226 16803-16876 sentence denotes A deep posterior diaphragmatic eventration was present on the left side.
T10227 16804-16805 DT denotes A
T10229 16806-16810 JJ denotes deep
T10230 16811-16820 JJ denotes posterior
T10231 16821-16834 JJ denotes diaphragmatic
T10228 16835-16846 NN denotes eventration
T10232 16847-16850 VBD denotes was
T10233 16851-16858 JJ denotes present
T10234 16859-16861 IN denotes on
T10235 16862-16865 DT denotes the
T10237 16866-16870 JJ denotes left
T10236 16871-16875 NN denotes side
T10238 16875-16876 . denotes .
T10239 16876-17042 sentence denotes Additionally, two muscularized ligamentous bands resembling diaphragmatic remnants encircled the left lobe of the liver, creating an abnormal fissured liver contour.
T10240 16877-16889 RB denotes Additionally
T10242 16889-16891 , denotes ,
T10243 16891-16894 CD denotes two
T10245 16895-16907 VBN denotes muscularized
T10246 16908-16919 JJ denotes ligamentous
T10244 16920-16925 NNS denotes bands
T10247 16926-16936 VBG denotes resembling
T10248 16937-16950 JJ denotes diaphragmatic
T10249 16951-16959 NNS denotes remnants
T10241 16960-16969 VBD denotes encircled
T10250 16970-16973 DT denotes the
T10252 16974-16978 JJ denotes left
T10251 16979-16983 NN denotes lobe
T10253 16984-16986 IN denotes of
T10254 16987-16990 DT denotes the
T10255 16991-16996 NN denotes liver
T10256 16996-16998 , denotes ,
T10257 16998-17006 VBG denotes creating
T10258 17007-17009 DT denotes an
T10260 17010-17018 JJ denotes abnormal
T10261 17019-17027 VBN denotes fissured
T10262 17028-17033 NN denotes liver
T10259 17034-17041 NN denotes contour
T10263 17041-17042 . denotes .
T10264 17042-17142 sentence denotes Away from the eventration, the diaphragm appeared well muscularized, measuring 0.3 cm in thickness.
T10265 17043-17047 RB denotes Away
T10267 17048-17052 IN denotes from
T10268 17053-17056 DT denotes the
T10269 17057-17068 NN denotes eventration
T10270 17068-17070 , denotes ,
T10271 17070-17073 DT denotes the
T10272 17074-17083 NN denotes diaphragm
T10266 17084-17092 VBD denotes appeared
T10273 17093-17097 RB denotes well
T10274 17098-17110 VBN denotes muscularized
T10275 17110-17112 , denotes ,
T10276 17112-17121 VBG denotes measuring
T10277 17122-17125 CD denotes 0.3
T10278 17126-17128 NN denotes cm
T10279 17129-17131 IN denotes in
T10280 17132-17141 NN denotes thickness
T10281 17141-17142 . denotes .
T10282 17142-17275 sentence denotes A complete autopsy revealed no other malformations; the heart was determined to be grossly normal and was donated for valve harvest.
T10283 17143-17144 DT denotes A
T10285 17145-17153 JJ denotes complete
T10284 17154-17161 NN denotes autopsy
T10286 17162-17170 VBD denotes revealed
T10288 17171-17173 DT denotes no
T10290 17174-17179 JJ denotes other
T10289 17180-17193 NNS denotes malformations
T10291 17193-17194 : denotes ;
T10292 17195-17198 DT denotes the
T10293 17199-17204 NN denotes heart
T10294 17205-17208 VBD denotes was
T10287 17209-17219 VBN denotes determined
T10295 17220-17222 TO denotes to
T10296 17223-17225 VB denotes be
T10297 17226-17233 RB denotes grossly
T10298 17234-17240 JJ denotes normal
T10299 17241-17244 CC denotes and
T10300 17245-17248 VBD denotes was
T10301 17249-17256 VBN denotes donated
T10302 17257-17260 IN denotes for
T10303 17261-17266 NN denotes valve
T10304 17267-17274 NN denotes harvest
T10305 17274-17275 . denotes .
T10306 17275-17419 sentence denotes Sequence analysis revealed a cytosine to thymine heterozygous change in exon 4 that changes the 112th amino acid from arginine to a stop codon.
T10307 17276-17284 NN denotes Sequence
T10308 17285-17293 NN denotes analysis
T10309 17294-17302 VBD denotes revealed
T10310 17303-17304 DT denotes a
T10312 17305-17313 NN denotes cytosine
T10313 17314-17316 IN denotes to
T10314 17317-17324 NN denotes thymine
T10315 17325-17337 JJ denotes heterozygous
T10311 17338-17344 NN denotes change
T10316 17345-17347 IN denotes in
T10317 17348-17352 NN denotes exon
T10318 17353-17354 CD denotes 4
T10319 17355-17359 WDT denotes that
T10320 17360-17367 VBZ denotes changes
T10321 17368-17371 DT denotes the
T10323 17372-17377 JJ denotes 112th
T10324 17378-17383 NN denotes amino
T10322 17384-17388 NN denotes acid
T10325 17389-17393 IN denotes from
T10326 17394-17402 NN denotes arginine
T10327 17403-17405 IN denotes to
T10328 17406-17407 DT denotes a
T10330 17408-17412 NN denotes stop
T10329 17413-17418 NN denotes codon
T10331 17418-17419 . denotes .
T10332 17419-17525 sentence denotes This mutation produces a severely truncated peptide that does not contain zinc finger domains (Figure 7).
T10333 17420-17424 DT denotes This
T10334 17425-17433 NN denotes mutation
T10335 17434-17442 VBZ denotes produces
T10336 17443-17444 DT denotes a
T10338 17445-17453 RB denotes severely
T10339 17454-17463 VBN denotes truncated
T10337 17464-17471 NN denotes peptide
T10340 17472-17476 WDT denotes that
T10342 17477-17481 VBZ denotes does
T10343 17482-17485 RB denotes not
T10341 17486-17493 VB denotes contain
T10344 17494-17498 NN denotes zinc
T10345 17499-17505 NN denotes finger
T10346 17506-17513 NNS denotes domains
T10347 17514-17515 -LRB- denotes (
T10348 17515-17521 NN denotes Figure
T10349 17522-17523 CD denotes 7
T10350 17523-17524 -RRB- denotes )
T10351 17524-17525 . denotes .
T10352 17525-17605 sentence denotes This base change was not present in the analysis of DNA from 400 normal adults.
T10353 17526-17530 DT denotes This
T10355 17531-17535 NN denotes base
T10354 17536-17542 NN denotes change
T10356 17543-17546 VBD denotes was
T10357 17547-17550 RB denotes not
T10358 17551-17558 JJ denotes present
T10359 17559-17561 IN denotes in
T10360 17562-17565 DT denotes the
T10361 17566-17574 NN denotes analysis
T10362 17575-17577 IN denotes of
T10363 17578-17581 NN denotes DNA
T10364 17582-17586 IN denotes from
T10365 17587-17590 CD denotes 400
T10367 17591-17597 JJ denotes normal
T10366 17598-17604 NNS denotes adults
T10368 17604-17605 . denotes .
T10369 17605-17718 sentence denotes To assess the likelihood that the mutation was causal for the developmental phenotype, we examined both parents.
T10370 17606-17608 TO denotes To
T10371 17609-17615 VB denotes assess
T10373 17616-17619 DT denotes the
T10374 17620-17630 NN denotes likelihood
T10375 17631-17635 IN denotes that
T10377 17636-17639 DT denotes the
T10378 17640-17648 NN denotes mutation
T10376 17649-17652 VBD denotes was
T10379 17653-17659 JJ denotes causal
T10380 17660-17663 IN denotes for
T10381 17664-17667 DT denotes the
T10383 17668-17681 JJ denotes developmental
T10382 17682-17691 NN denotes phenotype
T10384 17691-17693 , denotes ,
T10385 17693-17695 PRP denotes we
T10372 17696-17704 VBD denotes examined
T10386 17705-17709 DT denotes both
T10387 17710-17717 NNS denotes parents
T10388 17717-17718 . denotes .
T10389 17718-17889 sentence denotes Paternity was confirmed, and sequence analysis revealed that the neither parent carried this mutation, proving that the patient had a de novo mutation in FOG2 (Figure 7).
T10390 17719-17728 NN denotes Paternity
T10392 17729-17732 VBD denotes was
T10391 17733-17742 VBN denotes confirmed
T10393 17742-17744 , denotes ,
T10394 17744-17747 CC denotes and
T10395 17748-17756 NN denotes sequence
T10396 17757-17765 NN denotes analysis
T10397 17766-17774 VBD denotes revealed
T10398 17775-17779 IN denotes that
T10400 17780-17783 DT denotes the
T10402 17784-17791 CC denotes neither
T10401 17792-17798 NN denotes parent
T10399 17799-17806 VBD denotes carried
T10403 17807-17811 DT denotes this
T10404 17812-17820 NN denotes mutation
T10405 17820-17822 , denotes ,
T10406 17822-17829 VBG denotes proving
T10407 17830-17834 IN denotes that
T10409 17835-17838 DT denotes the
T10410 17839-17846 NN denotes patient
T10408 17847-17850 VBD denotes had
T10411 17851-17852 DT denotes a
T10413 17853-17855 FW denotes de
T10414 17856-17860 FW denotes novo
T10412 17861-17869 NN denotes mutation
T10415 17870-17872 IN denotes in
T10416 17873-17877 NN denotes FOG2
T10417 17878-17879 -LRB- denotes (
T10418 17879-17885 NN denotes Figure
T10419 17886-17887 CD denotes 7
T10420 17887-17888 -RRB- denotes )
T10421 17888-17889 . denotes .
T13037 18264-18274 JJ denotes Congenital
T13039 18275-18288 JJ denotes diaphragmatic
T13038 18289-18296 NNS denotes defects
T13040 18297-18300 VBP denotes are
T13041 18301-18302 DT denotes a
T13043 18303-18316 JJ denotes heterogeneous
T13042 18317-18322 NN denotes group
T13044 18323-18325 IN denotes of
T13045 18326-18335 NNS denotes disorders
T13046 18336-18338 IN denotes of
T13047 18339-18346 JJ denotes unknown
T13048 18347-18355 NN denotes etiology
T13049 18355-18356 . denotes .
T13050 18356-18526 sentence denotes The defects that present in the pre- or perinatal period include Bochdalek hernia, diaphragmatic aplasia, and various degrees of muscularization defects or eventrations.
T13051 18357-18360 DT denotes The
T13052 18361-18368 NNS denotes defects
T13054 18369-18373 WDT denotes that
T13055 18374-18381 VBP denotes present
T13056 18382-18384 IN denotes in
T13057 18385-18388 DT denotes the
T13059 18389-18392 AFX denotes pre
T13061 18392-18393 HYPH denotes -
T13062 18394-18396 CC denotes or
T13060 18397-18406 JJ denotes perinatal
T13058 18407-18413 NN denotes period
T13053 18414-18421 VBP denotes include
T13063 18422-18431 NNP denotes Bochdalek
T13064 18432-18438 NN denotes hernia
T13065 18438-18440 , denotes ,
T13066 18440-18453 JJ denotes diaphragmatic
T13067 18454-18461 NN denotes aplasia
T13068 18461-18463 , denotes ,
T13069 18463-18466 CC denotes and
T13070 18467-18474 JJ denotes various
T13071 18475-18482 NNS denotes degrees
T13072 18483-18485 IN denotes of
T13073 18486-18501 NN denotes muscularization
T13074 18502-18509 NNS denotes defects
T13075 18510-18512 CC denotes or
T13076 18513-18525 NNS denotes eventrations
T13077 18525-18526 . denotes .
T13078 18526-18688 sentence denotes Different types of defects occur in the same patients or in siblings, suggesting these represent variable expression of the same underlying pathogenesis [30,31].
T13079 18527-18536 JJ denotes Different
T13080 18537-18542 NNS denotes types
T13082 18543-18545 IN denotes of
T13083 18546-18553 NNS denotes defects
T13081 18554-18559 VBP denotes occur
T13084 18560-18562 IN denotes in
T13085 18563-18566 DT denotes the
T13087 18567-18571 JJ denotes same
T13086 18572-18580 NNS denotes patients
T13088 18581-18583 CC denotes or
T13089 18584-18586 IN denotes in
T13090 18587-18595 NNS denotes siblings
T13091 18595-18597 , denotes ,
T13092 18597-18607 VBG denotes suggesting
T13093 18608-18613 DT denotes these
T13094 18614-18623 VBP denotes represent
T13095 18624-18632 JJ denotes variable
T13096 18633-18643 NN denotes expression
T13097 18644-18646 IN denotes of
T13098 18647-18650 DT denotes the
T13100 18651-18655 JJ denotes same
T13101 18656-18666 VBG denotes underlying
T13099 18667-18679 NN denotes pathogenesis
T13102 18680-18681 -LRB- denotes [
T13104 18681-18683 CD denotes 30
T13105 18683-18684 , denotes ,
T13103 18684-18686 CD denotes 31
T13106 18686-18687 -RRB- denotes ]
T13107 18687-18688 . denotes .
T13108 18688-18921 sentence denotes Clinical differentiation between these defects may be very difficult, as the residual membranous diaphragm of a muscularization defect is thin and may not be easily visible on prenatal ultrasound or postnatal chest radiographs [32].
T13109 18689-18697 JJ denotes Clinical
T13110 18698-18713 NN denotes differentiation
T13112 18714-18721 IN denotes between
T13113 18722-18727 DT denotes these
T13114 18728-18735 NNS denotes defects
T13115 18736-18739 MD denotes may
T13111 18740-18742 VB denotes be
T13116 18743-18747 RB denotes very
T13117 18748-18757 JJ denotes difficult
T13118 18757-18759 , denotes ,
T13119 18759-18761 IN denotes as
T13121 18762-18765 DT denotes the
T13123 18766-18774 JJ denotes residual
T13124 18775-18785 JJ denotes membranous
T13122 18786-18795 NN denotes diaphragm
T13125 18796-18798 IN denotes of
T13126 18799-18800 DT denotes a
T13128 18801-18816 NN denotes muscularization
T13127 18817-18823 NN denotes defect
T13120 18824-18826 VBZ denotes is
T13129 18827-18831 JJ denotes thin
T13130 18832-18835 CC denotes and
T13131 18836-18839 MD denotes may
T13133 18840-18843 RB denotes not
T13132 18844-18846 VB denotes be
T13134 18847-18853 RB denotes easily
T13135 18854-18861 JJ denotes visible
T13136 18862-18864 IN denotes on
T13137 18865-18873 JJ denotes prenatal
T13138 18874-18884 JJ denotes ultrasound
T13139 18885-18887 CC denotes or
T13140 18888-18897 JJ denotes postnatal
T13142 18898-18903 NN denotes chest
T13141 18904-18915 NNS denotes radiographs
T13143 18916-18917 -LRB- denotes [
T13144 18917-18919 CD denotes 32
T13145 18919-18920 -RRB- denotes ]
T13146 18920-18921 . denotes .
T13147 18921-19180 sentence denotes Although diaphragmatic muscularization defects were historically considered to be predictive of a good outcome, there have been inadequate population-based studies that include fetal or neonatal cases and autopsy diagnoses to make this conclusion definitive.
T13148 18922-18930 IN denotes Although
T13150 18931-18944 JJ denotes diaphragmatic
T13152 18945-18960 NN denotes muscularization
T13151 18961-18968 NNS denotes defects
T13153 18969-18973 VBD denotes were
T13154 18974-18986 RB denotes historically
T13149 18987-18997 VBN denotes considered
T13156 18998-19000 TO denotes to
T13157 19001-19003 VB denotes be
T13158 19004-19014 JJ denotes predictive
T13159 19015-19017 IN denotes of
T13160 19018-19019 DT denotes a
T13162 19020-19024 JJ denotes good
T13161 19025-19032 NN denotes outcome
T13163 19032-19034 , denotes ,
T13164 19034-19039 EX denotes there
T13165 19040-19044 VBP denotes have
T13155 19045-19049 VBN denotes been
T13166 19050-19060 JJ denotes inadequate
T13168 19061-19071 NN denotes population
T13170 19071-19072 HYPH denotes -
T13169 19072-19077 VBN denotes based
T13167 19078-19085 NNS denotes studies
T13171 19086-19090 WDT denotes that
T13172 19091-19098 VBP denotes include
T13173 19099-19104 JJ denotes fetal
T13175 19105-19107 CC denotes or
T13176 19108-19116 JJ denotes neonatal
T13174 19117-19122 NNS denotes cases
T13177 19123-19126 CC denotes and
T13178 19127-19134 NN denotes autopsy
T13179 19135-19144 NNS denotes diagnoses
T13180 19145-19147 TO denotes to
T13181 19148-19152 VB denotes make
T13182 19153-19157 DT denotes this
T13183 19158-19168 NN denotes conclusion
T13184 19169-19179 JJ denotes definitive
T13185 19179-19180 . denotes .
T13186 19180-19431 sentence denotes In fact, the series of patients we report here and the published literature indicate that an eventration defect may be associated with displacement of abdominal contents and also with severe pulmonary hypoplasia and respiratory insufficiency [33,34].
T13187 19181-19183 IN denotes In
T13189 19184-19188 NN denotes fact
T13190 19188-19190 , denotes ,
T13191 19190-19193 DT denotes the
T13192 19194-19200 NN denotes series
T13193 19201-19203 IN denotes of
T13194 19204-19212 NNS denotes patients
T13195 19213-19215 PRP denotes we
T13196 19216-19222 VBP denotes report
T13197 19223-19227 RB denotes here
T13198 19228-19231 CC denotes and
T13199 19232-19235 DT denotes the
T13201 19236-19245 VBN denotes published
T13200 19246-19256 NN denotes literature
T13188 19257-19265 VBP denotes indicate
T13202 19266-19270 IN denotes that
T13204 19271-19273 DT denotes an
T13206 19274-19285 NN denotes eventration
T13205 19286-19292 NN denotes defect
T13207 19293-19296 MD denotes may
T13208 19297-19299 VB denotes be
T13203 19300-19310 VBN denotes associated
T13209 19311-19315 IN denotes with
T13210 19316-19328 NN denotes displacement
T13211 19329-19331 IN denotes of
T13212 19332-19341 JJ denotes abdominal
T13213 19342-19350 NNS denotes contents
T13214 19351-19354 CC denotes and
T13215 19355-19359 RB denotes also
T13216 19360-19364 IN denotes with
T13217 19365-19371 JJ denotes severe
T13219 19372-19381 JJ denotes pulmonary
T13218 19382-19392 NN denotes hypoplasia
T13220 19393-19396 CC denotes and
T13221 19397-19408 JJ denotes respiratory
T13222 19409-19422 NN denotes insufficiency
T13223 19423-19424 -LRB- denotes [
T13225 19424-19426 CD denotes 33
T13226 19426-19427 , denotes ,
T13224 19427-19429 CD denotes 34
T13227 19429-19430 -RRB- denotes ]
T13228 19430-19431 . denotes .
T13229 19431-19545 sentence denotes Numerous chromosome abnormalities have been found in association with congenital diaphragm abnormalities [12,35].
T13230 19432-19440 JJ denotes Numerous
T13232 19441-19451 NN denotes chromosome
T13231 19452-19465 NNS denotes abnormalities
T13234 19466-19470 VBP denotes have
T13235 19471-19475 VBN denotes been
T13233 19476-19481 VBN denotes found
T13236 19482-19484 IN denotes in
T13237 19485-19496 NN denotes association
T13238 19497-19501 IN denotes with
T13239 19502-19512 JJ denotes congenital
T13241 19513-19522 NN denotes diaphragm
T13240 19523-19536 NNS denotes abnormalities
T13242 19537-19538 -LRB- denotes [
T13244 19538-19540 CD denotes 12
T13245 19540-19541 , denotes ,
T13243 19541-19543 CD denotes 35
T13246 19543-19544 -RRB- denotes ]
T13247 19544-19545 . denotes .
T13248 19545-19701 sentence denotes Human FOG2 maps to Chromosome 8q23.1, and, importantly, several patients with diaphragm defects and rearrangements involving this locus have been reported.
T13249 19546-19551 JJ denotes Human
T13250 19552-19556 NN denotes FOG2
T13251 19557-19561 VBZ denotes maps
T13252 19562-19564 IN denotes to
T13253 19565-19575 NN denotes Chromosome
T13254 19576-19582 CD denotes 8q23.1
T13255 19582-19584 , denotes ,
T13256 19584-19587 CC denotes and
T13257 19587-19589 , denotes ,
T13258 19589-19600 RB denotes importantly
T13260 19600-19602 , denotes ,
T13261 19602-19609 JJ denotes several
T13262 19610-19618 NNS denotes patients
T13263 19619-19623 IN denotes with
T13264 19624-19633 NN denotes diaphragm
T13265 19634-19641 NNS denotes defects
T13266 19642-19645 CC denotes and
T13267 19646-19660 NNS denotes rearrangements
T13268 19661-19670 VBG denotes involving
T13269 19671-19675 DT denotes this
T13270 19676-19681 NN denotes locus
T13271 19682-19686 VBP denotes have
T13272 19687-19691 VBN denotes been
T13259 19692-19700 VBN denotes reported
T13273 19700-19701 . denotes .
T13274 19701-19834 sentence denotes Specifically, there are three unrelated CDH patients with cytogenetically balanced translocations at or near the FOG2 locus [36,37].
T13275 19702-19714 RB denotes Specifically
T13277 19714-19716 , denotes ,
T13278 19716-19721 EX denotes there
T13276 19722-19725 VBP denotes are
T13279 19726-19731 CD denotes three
T13281 19732-19741 JJ denotes unrelated
T13282 19742-19745 NN denotes CDH
T13280 19746-19754 NNS denotes patients
T13283 19755-19759 IN denotes with
T13284 19760-19775 RB denotes cytogenetically
T13285 19776-19784 VBN denotes balanced
T13286 19785-19799 NNS denotes translocations
T13287 19800-19802 IN denotes at
T13288 19803-19805 CC denotes or
T13289 19806-19810 IN denotes near
T13290 19811-19814 DT denotes the
T13292 19815-19819 NN denotes FOG2
T13291 19820-19825 NN denotes locus
T13293 19826-19827 -LRB- denotes [
T13295 19827-19829 CD denotes 36
T13296 19829-19830 , denotes ,
T13294 19830-19832 CD denotes 37
T13297 19832-19833 -RRB- denotes ]
T13298 19833-19834 . denotes .
T13299 19834-19983 sentence denotes Additionally, two patients with deletions apparently encompassing the FOG2 locus have died from multiple congenital anomalies including CDH [38–40].
T13300 19835-19847 RB denotes Additionally
T13302 19847-19849 , denotes ,
T13303 19849-19852 CD denotes two
T13304 19853-19861 NNS denotes patients
T13305 19862-19866 IN denotes with
T13306 19867-19876 NNS denotes deletions
T13307 19877-19887 RB denotes apparently
T13308 19888-19900 VBG denotes encompassing
T13309 19901-19904 DT denotes the
T13311 19905-19909 NN denotes FOG2
T13310 19910-19915 NN denotes locus
T13312 19916-19920 VBP denotes have
T13301 19921-19925 VBN denotes died
T13313 19926-19930 IN denotes from
T13314 19931-19939 JJ denotes multiple
T13316 19940-19950 JJ denotes congenital
T13315 19951-19960 NNS denotes anomalies
T13317 19961-19970 VBG denotes including
T13318 19971-19974 NN denotes CDH
T13319 19975-19976 -LRB- denotes [
T13320 19976-19978 CD denotes 38
T13321 19978-19979 SYM denotes –
T13322 19979-19981 CD denotes 40
T13323 19981-19982 -RRB- denotes ]
T13324 19982-19983 . denotes .
T13325 19983-20147 sentence denotes Inactivation of this gene due to chromosomal rearrangement or deletion would result in a heterozygous null mutation similar to that found in the patient we report.
T13326 19984-19996 NN denotes Inactivation
T13328 19997-19999 IN denotes of
T13329 20000-20004 DT denotes this
T13330 20005-20009 NN denotes gene
T13331 20010-20013 IN denotes due
T13332 20014-20016 IN denotes to
T13333 20017-20028 JJ denotes chromosomal
T13334 20029-20042 NN denotes rearrangement
T13335 20043-20045 CC denotes or
T13336 20046-20054 NN denotes deletion
T13337 20055-20060 MD denotes would
T13327 20061-20067 VB denotes result
T13338 20068-20070 IN denotes in
T13339 20071-20072 DT denotes a
T13341 20073-20085 JJ denotes heterozygous
T13342 20086-20090 JJ denotes null
T13340 20091-20099 NN denotes mutation
T13343 20100-20107 JJ denotes similar
T13344 20108-20110 IN denotes to
T13345 20111-20115 DT denotes that
T13346 20116-20121 VBN denotes found
T13347 20122-20124 IN denotes in
T13348 20125-20128 DT denotes the
T13349 20129-20136 NN denotes patient
T13350 20137-20139 PRP denotes we
T13351 20140-20146 VBP denotes report
T13352 20146-20147 . denotes .
T13353 20147-20420 sentence denotes Because the FOG2 mutation we report is de novo and the phenotypes of the pulmonary and diaphragmatic defects are similar between mouse and human, we suggest that this mutation in FOG2 is the first reported cause of a human developmental diaphragmatic and pulmonary defect.
T13354 20148-20155 IN denotes Because
T13356 20156-20159 DT denotes the
T13358 20160-20164 NN denotes FOG2
T13357 20165-20173 NN denotes mutation
T13359 20174-20176 PRP denotes we
T13360 20177-20183 VBP denotes report
T13355 20184-20186 VBZ denotes is
T13362 20187-20189 FW denotes de
T13363 20190-20194 FW denotes novo
T13364 20195-20198 CC denotes and
T13365 20199-20202 DT denotes the
T13366 20203-20213 NNS denotes phenotypes
T13368 20214-20216 IN denotes of
T13369 20217-20220 DT denotes the
T13371 20221-20230 JJ denotes pulmonary
T13372 20231-20234 CC denotes and
T13373 20235-20248 JJ denotes diaphragmatic
T13370 20249-20256 NNS denotes defects
T13367 20257-20260 VBP denotes are
T13374 20261-20268 JJ denotes similar
T13375 20269-20276 IN denotes between
T13376 20277-20282 NN denotes mouse
T13377 20283-20286 CC denotes and
T13378 20287-20292 JJ denotes human
T13379 20292-20294 , denotes ,
T13380 20294-20296 PRP denotes we
T13361 20297-20304 VBP denotes suggest
T13381 20305-20309 IN denotes that
T13383 20310-20314 DT denotes this
T13384 20315-20323 NN denotes mutation
T13385 20324-20326 IN denotes in
T13386 20327-20331 NN denotes FOG2
T13382 20332-20334 VBZ denotes is
T13387 20335-20338 DT denotes the
T13389 20339-20344 JJ denotes first
T13390 20345-20353 VBN denotes reported
T13388 20354-20359 NN denotes cause
T13391 20360-20362 IN denotes of
T13392 20363-20364 DT denotes a
T13394 20365-20370 JJ denotes human
T13395 20371-20384 JJ denotes developmental
T13396 20385-20398 JJ denotes diaphragmatic
T13397 20399-20402 CC denotes and
T13398 20403-20412 JJ denotes pulmonary
T13393 20413-20419 NN denotes defect
T13399 20419-20420 . denotes .
T13400 20420-20516 sentence denotes In contrast to the affected child, mice heterozygous for a null mutation of Fog2 appear normal.
T13401 20421-20423 IN denotes In
T13403 20424-20432 NN denotes contrast
T13404 20433-20435 IN denotes to
T13405 20436-20439 DT denotes the
T13407 20440-20448 VBN denotes affected
T13406 20449-20454 NN denotes child
T13408 20454-20456 , denotes ,
T13409 20456-20460 NNS denotes mice
T13410 20461-20473 JJ denotes heterozygous
T13411 20474-20477 IN denotes for
T13412 20478-20479 DT denotes a
T13414 20480-20484 JJ denotes null
T13413 20485-20493 NN denotes mutation
T13415 20494-20496 IN denotes of
T13416 20497-20501 NN denotes Fog2
T13402 20502-20508 VBP denotes appear
T13417 20509-20515 JJ denotes normal
T13418 20515-20516 . denotes .
T13419 20516-20687 sentence denotes However, there is ample precedent for the observation that haploinsufficiency of a gene with developmental functions is much less well tolerated in humans than mice [41].
T13420 20517-20524 RB denotes However
T13422 20524-20526 , denotes ,
T13423 20526-20531 EX denotes there
T13421 20532-20534 VBZ denotes is
T13424 20535-20540 JJ denotes ample
T13425 20541-20550 NN denotes precedent
T13426 20551-20554 IN denotes for
T13427 20555-20558 DT denotes the
T13428 20559-20570 NN denotes observation
T13429 20571-20575 IN denotes that
T13431 20576-20594 NN denotes haploinsufficiency
T13432 20595-20597 IN denotes of
T13433 20598-20599 DT denotes a
T13434 20600-20604 NN denotes gene
T13435 20605-20609 IN denotes with
T13436 20610-20623 JJ denotes developmental
T13437 20624-20633 NNS denotes functions
T13430 20634-20636 VBZ denotes is
T13438 20637-20641 RB denotes much
T13439 20642-20646 RBR denotes less
T13440 20647-20651 RB denotes well
T13441 20652-20661 VBN denotes tolerated
T13442 20662-20664 IN denotes in
T13443 20665-20671 NNS denotes humans
T13444 20672-20676 IN denotes than
T13445 20677-20681 NNS denotes mice
T13446 20682-20683 -LRB- denotes [
T13447 20683-20685 CD denotes 41
T13448 20685-20686 -RRB- denotes ]
T13449 20686-20687 . denotes .
T13450 20687-20836 sentence denotes It is unclear how the Fog2 diaphragmatic defect relates to the more common Bochdalek CDH, as the pathogenic mechanisms for both are largely unknown.
T13451 20688-20690 PRP denotes It
T13452 20691-20693 VBZ denotes is
T13453 20694-20701 JJ denotes unclear
T13454 20702-20705 WRB denotes how
T13456 20706-20709 DT denotes the
T13458 20710-20714 NN denotes Fog2
T13459 20715-20728 JJ denotes diaphragmatic
T13457 20729-20735 NN denotes defect
T13455 20736-20743 VBZ denotes relates
T13460 20744-20746 IN denotes to
T13461 20747-20750 DT denotes the
T13463 20751-20755 RBR denotes more
T13464 20756-20762 JJ denotes common
T13465 20763-20772 NNP denotes Bochdalek
T13462 20773-20776 NN denotes CDH
T13466 20776-20778 , denotes ,
T13467 20778-20780 IN denotes as
T13469 20781-20784 DT denotes the
T13471 20785-20795 JJ denotes pathogenic
T13470 20796-20806 NNS denotes mechanisms
T13472 20807-20810 IN denotes for
T13473 20811-20815 DT denotes both
T13468 20816-20819 VBP denotes are
T13474 20820-20827 RB denotes largely
T13475 20828-20835 JJ denotes unknown
T13476 20835-20836 . denotes .
T13477 20836-20948 sentence denotes Muscle precursors destined to populate the diaphragm migrate from the lateral dermomyotome of cervical somites.
T13478 20837-20843 NN denotes Muscle
T13479 20844-20854 NNS denotes precursors
T13481 20855-20863 VBN denotes destined
T13482 20864-20866 TO denotes to
T13483 20867-20875 VB denotes populate
T13484 20876-20879 DT denotes the
T13485 20880-20889 NN denotes diaphragm
T13480 20890-20897 VBP denotes migrate
T13486 20898-20902 IN denotes from
T13487 20903-20906 DT denotes the
T13489 20907-20914 JJ denotes lateral
T13488 20915-20927 NN denotes dermomyotome
T13490 20928-20930 IN denotes of
T13491 20931-20939 JJ denotes cervical
T13492 20940-20947 NNS denotes somites
T13493 20947-20948 . denotes .
T13494 20948-21162 sentence denotes Prior to migration onto the diaphragm, they populate the PPF, a wedge-shaped tissue that tapers medially from the lateral body wall to the esophageal mesentery and fuses ventrally with the septum transversum [42].
T13495 20949-20954 RB denotes Prior
T13496 20955-20957 IN denotes to
T13498 20958-20967 NN denotes migration
T13499 20968-20972 IN denotes onto
T13500 20973-20976 DT denotes the
T13501 20977-20986 NN denotes diaphragm
T13502 20986-20988 , denotes ,
T13503 20988-20992 PRP denotes they
T13497 20993-21001 VBP denotes populate
T13504 21002-21005 DT denotes the
T13505 21006-21009 NN denotes PPF
T13506 21009-21011 , denotes ,
T13507 21011-21012 DT denotes a
T13509 21013-21018 NN denotes wedge
T13511 21018-21019 HYPH denotes -
T13510 21019-21025 VBN denotes shaped
T13508 21026-21032 NN denotes tissue
T13512 21033-21037 WDT denotes that
T13513 21038-21044 VBZ denotes tapers
T13514 21045-21053 RB denotes medially
T13515 21054-21058 IN denotes from
T13516 21059-21062 DT denotes the
T13518 21063-21070 JJ denotes lateral
T13519 21071-21075 NN denotes body
T13517 21076-21080 NN denotes wall
T13520 21081-21083 IN denotes to
T13521 21084-21087 DT denotes the
T13523 21088-21098 JJ denotes esophageal
T13522 21099-21108 NN denotes mesentery
T13524 21109-21112 CC denotes and
T13525 21113-21118 VBZ denotes fuses
T13526 21119-21128 RB denotes ventrally
T13527 21129-21133 IN denotes with
T13528 21134-21137 DT denotes the
T13530 21138-21144 NN denotes septum
T13529 21145-21156 NN denotes transversum
T13531 21157-21158 -LRB- denotes [
T13532 21158-21160 CD denotes 42
T13533 21160-21161 -RRB- denotes ]
T13534 21161-21162 . denotes .
T13535 21162-21354 sentence denotes Muscle precursors reach the PPF by E11, where they proliferate, differentiate, and then migrate toward the dorsolateral costal, sternal costal, and crural regions of the developing diaphragm.
T13536 21163-21169 NN denotes Muscle
T13537 21170-21180 NNS denotes precursors
T13538 21181-21186 VBP denotes reach
T13539 21187-21190 DT denotes the
T13540 21191-21194 NN denotes PPF
T13541 21195-21197 IN denotes by
T13542 21198-21201 NN denotes E11
T13543 21201-21203 , denotes ,
T13544 21203-21208 WRB denotes where
T13546 21209-21213 PRP denotes they
T13545 21214-21225 VBP denotes proliferate
T13547 21225-21227 , denotes ,
T13548 21227-21240 VBP denotes differentiate
T13549 21240-21242 , denotes ,
T13550 21242-21245 CC denotes and
T13551 21246-21250 RB denotes then
T13552 21251-21258 VBP denotes migrate
T13553 21259-21265 IN denotes toward
T13554 21266-21269 DT denotes the
T13556 21270-21282 JJ denotes dorsolateral
T13557 21283-21289 JJ denotes costal
T13558 21289-21291 , denotes ,
T13559 21291-21298 JJ denotes sternal
T13560 21299-21305 JJ denotes costal
T13561 21305-21307 , denotes ,
T13562 21307-21310 CC denotes and
T13563 21311-21317 JJ denotes crural
T13555 21318-21325 NNS denotes regions
T13564 21326-21328 IN denotes of
T13565 21329-21332 DT denotes the
T13567 21333-21343 VBG denotes developing
T13566 21344-21353 NN denotes diaphragm
T13568 21353-21354 . denotes .
T13569 21354-21456 sentence denotes Thus, a defect in PPF formation subsequently results in the abnormal formation of the diaphragm [43].
T13570 21355-21359 RB denotes Thus
T13572 21359-21361 , denotes ,
T13573 21361-21362 DT denotes a
T13574 21363-21369 NN denotes defect
T13575 21370-21372 IN denotes in
T13576 21373-21376 NN denotes PPF
T13577 21377-21386 NN denotes formation
T13578 21387-21399 RB denotes subsequently
T13571 21400-21407 VBZ denotes results
T13579 21408-21410 IN denotes in
T13580 21411-21414 DT denotes the
T13582 21415-21423 JJ denotes abnormal
T13581 21424-21433 NN denotes formation
T13583 21434-21436 IN denotes of
T13584 21437-21440 DT denotes the
T13585 21441-21450 NN denotes diaphragm
T13586 21451-21452 -LRB- denotes [
T13587 21452-21454 CD denotes 43
T13588 21454-21455 -RRB- denotes ]
T13589 21455-21456 . denotes .
T13590 21456-21629 sentence denotes We have shown that the Fog2 mutant does have an abnormal pattern of HGF expression in the region through which muscle precursor cells migrate onto the developing diaphragm.
T13591 21457-21459 PRP denotes We
T13593 21460-21464 VBP denotes have
T13592 21465-21470 VBN denotes shown
T13594 21471-21475 IN denotes that
T13596 21476-21479 DT denotes the
T13598 21480-21484 NN denotes Fog2
T13597 21485-21491 NN denotes mutant
T13599 21492-21496 VBZ denotes does
T13595 21497-21501 VB denotes have
T13600 21502-21504 DT denotes an
T13602 21505-21513 JJ denotes abnormal
T13601 21514-21521 NN denotes pattern
T13603 21522-21524 IN denotes of
T13604 21525-21528 NN denotes HGF
T13605 21529-21539 NN denotes expression
T13606 21540-21542 IN denotes in
T13607 21543-21546 DT denotes the
T13608 21547-21553 NN denotes region
T13609 21554-21561 IN denotes through
T13611 21562-21567 WDT denotes which
T13612 21568-21574 NN denotes muscle
T13614 21575-21584 NN denotes precursor
T13613 21585-21590 NNS denotes cells
T13610 21591-21598 VBP denotes migrate
T13615 21599-21603 IN denotes onto
T13616 21604-21607 DT denotes the
T13618 21608-21618 VBG denotes developing
T13617 21619-21628 NN denotes diaphragm
T13619 21628-21629 . denotes .
T13620 21629-21734 sentence denotes This finding may account for the abnormally patterned muscle that develops in the Fog2 mutant diaphragm.
T13621 21630-21634 DT denotes This
T13622 21635-21642 NN denotes finding
T13624 21643-21646 MD denotes may
T13623 21647-21654 VB denotes account
T13625 21655-21658 IN denotes for
T13626 21659-21662 DT denotes the
T13628 21663-21673 RB denotes abnormally
T13629 21674-21683 VBN denotes patterned
T13627 21684-21690 NN denotes muscle
T13630 21691-21695 WDT denotes that
T13631 21696-21704 VBZ denotes develops
T13632 21705-21707 IN denotes in
T13633 21708-21711 DT denotes the
T13635 21712-21716 NN denotes Fog2
T13636 21717-21723 NN denotes mutant
T13634 21724-21733 NN denotes diaphragm
T13637 21733-21734 . denotes .
T13638 21734-22050 sentence denotes Although Pax3 and MyoD expression is detected in the PPF, a detailed analysis of transcription factors responsible for muscle precursor cell migration and differentiation will need to be completed both in the PPF and along the pathway of muscle precursor cell migration between the PPF and the membranous diaphragm.
T13639 21735-21743 IN denotes Although
T13641 21744-21748 NN denotes Pax3
T13643 21749-21752 CC denotes and
T13644 21753-21757 NN denotes MyoD
T13642 21758-21768 NN denotes expression
T13645 21769-21771 VBZ denotes is
T13640 21772-21780 VBN denotes detected
T13647 21781-21783 IN denotes in
T13648 21784-21787 DT denotes the
T13649 21788-21791 NN denotes PPF
T13650 21791-21793 , denotes ,
T13651 21793-21794 DT denotes a
T13653 21795-21803 JJ denotes detailed
T13652 21804-21812 NN denotes analysis
T13654 21813-21815 IN denotes of
T13655 21816-21829 NN denotes transcription
T13656 21830-21837 NNS denotes factors
T13657 21838-21849 JJ denotes responsible
T13658 21850-21853 IN denotes for
T13659 21854-21860 NN denotes muscle
T13661 21861-21870 NN denotes precursor
T13660 21871-21875 NN denotes cell
T13662 21876-21885 NN denotes migration
T13663 21886-21889 CC denotes and
T13664 21890-21905 NN denotes differentiation
T13665 21906-21910 MD denotes will
T13646 21911-21915 VB denotes need
T13666 21916-21918 TO denotes to
T13668 21919-21921 VB denotes be
T13667 21922-21931 VBN denotes completed
T13669 21932-21936 CC denotes both
T13670 21937-21939 IN denotes in
T13671 21940-21943 DT denotes the
T13672 21944-21947 NN denotes PPF
T13673 21948-21951 CC denotes and
T13674 21952-21957 IN denotes along
T13675 21958-21961 DT denotes the
T13676 21962-21969 NN denotes pathway
T13677 21970-21972 IN denotes of
T13678 21973-21979 NN denotes muscle
T13680 21980-21989 NN denotes precursor
T13679 21990-21994 NN denotes cell
T13681 21995-22004 NN denotes migration
T13682 22005-22012 IN denotes between
T13683 22013-22016 DT denotes the
T13684 22017-22020 NN denotes PPF
T13685 22021-22024 CC denotes and
T13686 22025-22028 DT denotes the
T13688 22029-22039 JJ denotes membranous
T13687 22040-22049 NN denotes diaphragm
T13689 22049-22050 . denotes .
T13690 22050-22174 sentence denotes Fog2 can interact with any of the Gata factors, Gatas 1–6, as well as other transcription factors such as CoupTFII [44,45].
T13691 22051-22055 NN denotes Fog2
T13693 22056-22059 MD denotes can
T13692 22060-22068 VB denotes interact
T13694 22069-22073 IN denotes with
T13695 22074-22077 DT denotes any
T13696 22078-22080 IN denotes of
T13697 22081-22084 DT denotes the
T13699 22085-22089 NN denotes Gata
T13698 22090-22097 NNS denotes factors
T13700 22097-22099 , denotes ,
T13701 22099-22104 NNS denotes Gatas
T13702 22105-22106 CD denotes 1
T13703 22106-22107 SYM denotes –
T13704 22107-22108 CD denotes 6
T13705 22108-22110 , denotes ,
T13706 22110-22112 RB denotes as
T13708 22113-22117 RB denotes well
T13707 22118-22120 IN denotes as
T13709 22121-22126 JJ denotes other
T13711 22127-22140 NN denotes transcription
T13710 22141-22148 NNS denotes factors
T13712 22149-22153 JJ denotes such
T13713 22154-22156 IN denotes as
T13714 22157-22165 NN denotes CoupTFII
T13715 22166-22167 -LRB- denotes [
T13717 22167-22169 CD denotes 44
T13718 22169-22170 , denotes ,
T13716 22170-22172 CD denotes 45
T13719 22172-22173 -RRB- denotes ]
T13720 22173-22174 . denotes .
T13721 22174-22352 sentence denotes It is known that a Fog2–Gata4 interaction is critical for normal cardiac and gonadal development, but interacting factors in the lung and diaphragm have not yet been determined.
T13722 22175-22177 PRP denotes It
T13724 22178-22180 VBZ denotes is
T13723 22181-22186 VBN denotes known
T13725 22187-22191 IN denotes that
T13727 22192-22193 DT denotes a
T13729 22194-22198 NN denotes Fog2
T13731 22198-22199 HYPH denotes –
T13730 22199-22204 NN denotes Gata4
T13728 22205-22216 NN denotes interaction
T13726 22217-22219 VBZ denotes is
T13732 22220-22228 JJ denotes critical
T13733 22229-22232 IN denotes for
T13734 22233-22239 JJ denotes normal
T13736 22240-22247 JJ denotes cardiac
T13737 22248-22251 CC denotes and
T13738 22252-22259 JJ denotes gonadal
T13735 22260-22271 NN denotes development
T13739 22271-22273 , denotes ,
T13740 22273-22276 CC denotes but
T13741 22277-22288 VBG denotes interacting
T13742 22289-22296 NNS denotes factors
T13744 22297-22299 IN denotes in
T13745 22300-22303 DT denotes the
T13746 22304-22308 NN denotes lung
T13747 22309-22312 CC denotes and
T13748 22313-22322 NN denotes diaphragm
T13749 22323-22327 VBP denotes have
T13750 22328-22331 RB denotes not
T13751 22332-22335 RB denotes yet
T13752 22336-22340 VBN denotes been
T13743 22341-22351 VBN denotes determined
T13753 22351-22352 . denotes .
T13754 22352-22469 sentence denotes The severity of pulmonary hypoplasia in the patient we report was out of proportion to that of the diaphragm defect.
T13755 22353-22356 DT denotes The
T13756 22357-22365 NN denotes severity
T13758 22366-22368 IN denotes of
T13759 22369-22378 JJ denotes pulmonary
T13760 22379-22389 NN denotes hypoplasia
T13761 22390-22392 IN denotes in
T13762 22393-22396 DT denotes the
T13763 22397-22404 NN denotes patient
T13764 22405-22407 PRP denotes we
T13765 22408-22414 VBP denotes report
T13757 22415-22418 VBD denotes was
T13766 22419-22422 IN denotes out
T13767 22423-22425 IN denotes of
T13768 22426-22436 NN denotes proportion
T13769 22437-22439 IN denotes to
T13770 22440-22444 DT denotes that
T13771 22445-22447 IN denotes of
T13772 22448-22451 DT denotes the
T13774 22452-22461 NN denotes diaphragm
T13773 22462-22468 NN denotes defect
T13775 22468-22469 . denotes .
T13776 22469-22723 sentence denotes Pulmonary hypoplasia is associated with abnormal diaphragmatic anatomy or function, and is known to occur as a secondary developmental defect in models of diaphragmatic dysfunction such as complete amuscularization [17] or phrenic nerve disruption [46].
T13777 22470-22479 JJ denotes Pulmonary
T13778 22480-22490 NN denotes hypoplasia
T13779 22491-22493 VBZ denotes is
T13780 22494-22504 JJ denotes associated
T13781 22505-22509 IN denotes with
T13782 22510-22518 JJ denotes abnormal
T13784 22519-22532 JJ denotes diaphragmatic
T13783 22533-22540 NN denotes anatomy
T13785 22541-22543 CC denotes or
T13786 22544-22552 NN denotes function
T13787 22552-22554 , denotes ,
T13788 22554-22557 CC denotes and
T13789 22558-22560 VBZ denotes is
T13790 22561-22566 VBN denotes known
T13791 22567-22569 TO denotes to
T13792 22570-22575 VB denotes occur
T13793 22576-22578 IN denotes as
T13794 22579-22580 DT denotes a
T13796 22581-22590 JJ denotes secondary
T13797 22591-22604 JJ denotes developmental
T13795 22605-22611 NN denotes defect
T13798 22612-22614 IN denotes in
T13799 22615-22621 NNS denotes models
T13800 22622-22624 IN denotes of
T13801 22625-22638 JJ denotes diaphragmatic
T13802 22639-22650 NN denotes dysfunction
T13803 22651-22655 JJ denotes such
T13804 22656-22658 IN denotes as
T13805 22659-22667 JJ denotes complete
T13806 22668-22684 NN denotes amuscularization
T13807 22685-22686 -LRB- denotes [
T13808 22686-22688 CD denotes 17
T13809 22688-22689 -RRB- denotes ]
T13810 22690-22692 CC denotes or
T13811 22693-22700 JJ denotes phrenic
T13812 22701-22706 NN denotes nerve
T13813 22707-22717 NN denotes disruption
T13814 22718-22719 -LRB- denotes [
T13815 22719-22721 CD denotes 46
T13816 22721-22722 -RRB- denotes ]
T13817 22722-22723 . denotes .
T13818 22723-22829 sentence denotes It occurs in a surgical model of CDH in which a hernia is physically created in an in utero lamb [47,48].
T13819 22724-22726 PRP denotes It
T13820 22727-22733 VBZ denotes occurs
T13821 22734-22736 IN denotes in
T13822 22737-22738 DT denotes a
T13824 22739-22747 JJ denotes surgical
T13823 22748-22753 NN denotes model
T13825 22754-22756 IN denotes of
T13826 22757-22760 NN denotes CDH
T13827 22761-22763 IN denotes in
T13829 22764-22769 WDT denotes which
T13830 22770-22771 DT denotes a
T13831 22772-22778 NN denotes hernia
T13832 22779-22781 VBZ denotes is
T13833 22782-22792 RB denotes physically
T13828 22793-22800 VBN denotes created
T13834 22801-22803 IN denotes in
T13835 22804-22806 DT denotes an
T13837 22807-22809 FW denotes in
T13838 22810-22815 FW denotes utero
T13836 22816-22820 NN denotes lamb
T13839 22821-22822 -LRB- denotes [
T13841 22822-22824 CD denotes 47
T13842 22824-22825 , denotes ,
T13840 22825-22827 CD denotes 48
T13843 22827-22828 -RRB- denotes ]
T13844 22828-22829 . denotes .
T13845 22829-23109 sentence denotes However, the possibility that primary pulmonary developmental abnormalities occur with, rather than secondary to, diaphragmatic defects has been suggested by others based on a teratogenic model of CDH [49–51] and has long been suspected by clinicians who care for these patients.
T13846 22830-22837 RB denotes However
T13848 22837-22839 , denotes ,
T13849 22839-22842 DT denotes the
T13850 22843-22854 NN denotes possibility
T13851 22855-22859 IN denotes that
T13853 22860-22867 JJ denotes primary
T13855 22868-22877 JJ denotes pulmonary
T13856 22878-22891 JJ denotes developmental
T13854 22892-22905 NNS denotes abnormalities
T13852 22906-22911 VBP denotes occur
T13857 22912-22916 IN denotes with
T13858 22916-22918 , denotes ,
T13859 22918-22924 RB denotes rather
T13860 22925-22929 IN denotes than
T13861 22930-22939 RB denotes secondary
T13862 22940-22942 IN denotes to
T13863 22942-22944 , denotes ,
T13864 22944-22957 JJ denotes diaphragmatic
T13865 22958-22965 NNS denotes defects
T13866 22966-22969 VBZ denotes has
T13867 22970-22974 VBN denotes been
T13847 22975-22984 VBN denotes suggested
T13868 22985-22987 IN denotes by
T13869 22988-22994 NNS denotes others
T13870 22995-23000 VBN denotes based
T13871 23001-23003 IN denotes on
T13872 23004-23005 DT denotes a
T13874 23006-23017 JJ denotes teratogenic
T13873 23018-23023 NN denotes model
T13875 23024-23026 IN denotes of
T13876 23027-23030 NN denotes CDH
T13877 23031-23032 -LRB- denotes [
T13878 23032-23034 CD denotes 49
T13879 23034-23035 SYM denotes –
T13880 23035-23037 CD denotes 51
T13881 23037-23038 -RRB- denotes ]
T13882 23039-23042 CC denotes and
T13883 23043-23046 VBZ denotes has
T13885 23047-23051 RB denotes long
T13886 23052-23056 VBN denotes been
T13884 23057-23066 VBN denotes suspected
T13887 23067-23069 IN denotes by
T13888 23070-23080 NNS denotes clinicians
T13889 23081-23084 WP denotes who
T13890 23085-23089 VBP denotes care
T13891 23090-23093 IN denotes for
T13892 23094-23099 DT denotes these
T13893 23100-23108 NNS denotes patients
T13894 23108-23109 . denotes .
T13895 23109-23303 sentence denotes In addition, the high incidence of lobar abnormalities associated with CDH [52] supports the possibility that this disorder can be associated with a primary developmental pulmonary abnormality.
T13896 23110-23112 IN denotes In
T13898 23113-23121 NN denotes addition
T13899 23121-23123 , denotes ,
T13900 23123-23126 DT denotes the
T13902 23127-23131 JJ denotes high
T13901 23132-23141 NN denotes incidence
T13903 23142-23144 IN denotes of
T13904 23145-23150 NN denotes lobar
T13905 23151-23164 NNS denotes abnormalities
T13906 23165-23175 JJ denotes associated
T13907 23176-23180 IN denotes with
T13908 23181-23184 NN denotes CDH
T13909 23185-23186 -LRB- denotes [
T13910 23186-23188 CD denotes 52
T13911 23188-23189 -RRB- denotes ]
T13897 23190-23198 VBZ denotes supports
T13912 23199-23202 DT denotes the
T13913 23203-23214 NN denotes possibility
T13914 23215-23219 IN denotes that
T13916 23220-23224 DT denotes this
T13917 23225-23233 NN denotes disorder
T13918 23234-23237 MD denotes can
T13919 23238-23240 VB denotes be
T13915 23241-23251 VBN denotes associated
T13920 23252-23256 IN denotes with
T13921 23257-23258 DT denotes a
T13923 23259-23266 JJ denotes primary
T13924 23267-23280 JJ denotes developmental
T13925 23281-23290 JJ denotes pulmonary
T13922 23291-23302 NN denotes abnormality
T13926 23302-23303 . denotes .
T13927 23303-23504 sentence denotes Our analysis of mice carrying mutations of Fog2 proves that there is a primary defect in lung development that results in specific loss of the accessory lobe and partial loss of the right middle lobe.
T13928 23304-23307 PRP$ denotes Our
T13929 23308-23316 NN denotes analysis
T13931 23317-23319 IN denotes of
T13932 23320-23324 NNS denotes mice
T13933 23325-23333 VBG denotes carrying
T13934 23334-23343 NNS denotes mutations
T13935 23344-23346 IN denotes of
T13936 23347-23351 NN denotes Fog2
T13930 23352-23358 VBZ denotes proves
T13937 23359-23363 IN denotes that
T13939 23364-23369 EX denotes there
T13938 23370-23372 VBZ denotes is
T13940 23373-23374 DT denotes a
T13942 23375-23382 JJ denotes primary
T13941 23383-23389 NN denotes defect
T13943 23390-23392 IN denotes in
T13944 23393-23397 NN denotes lung
T13945 23398-23409 NN denotes development
T13946 23410-23414 WDT denotes that
T13947 23415-23422 VBZ denotes results
T13948 23423-23425 IN denotes in
T13949 23426-23434 JJ denotes specific
T13950 23435-23439 NN denotes loss
T13951 23440-23442 IN denotes of
T13952 23443-23446 DT denotes the
T13954 23447-23456 JJ denotes accessory
T13953 23457-23461 NN denotes lobe
T13955 23462-23465 CC denotes and
T13956 23466-23473 JJ denotes partial
T13957 23474-23478 NN denotes loss
T13958 23479-23481 IN denotes of
T13959 23482-23485 DT denotes the
T13961 23486-23491 JJ denotes right
T13962 23492-23498 JJ denotes middle
T13960 23499-23503 NN denotes lobe
T13963 23503-23504 . denotes .
T13964 23504-23607 sentence denotes The specific lobar defects prompted us to evaluate Fog2 expression at the time of early lobar budding.
T13965 23505-23508 DT denotes The
T13967 23509-23517 JJ denotes specific
T13968 23518-23523 NN denotes lobar
T13966 23524-23531 NNS denotes defects
T13969 23532-23540 VBD denotes prompted
T13970 23541-23543 PRP denotes us
T13971 23544-23546 TO denotes to
T13972 23547-23555 VB denotes evaluate
T13973 23556-23560 NN denotes Fog2
T13974 23561-23571 NN denotes expression
T13975 23572-23574 IN denotes at
T13976 23575-23578 DT denotes the
T13977 23579-23583 NN denotes time
T13978 23584-23586 IN denotes of
T13979 23587-23592 JJ denotes early
T13981 23593-23598 NN denotes lobar
T13980 23599-23606 NN denotes budding
T13982 23606-23607 . denotes .
T13983 23607-23840 sentence denotes While Fog2 expression is diffuse in the pulmonary mesenchyme after lobar structure is well established (E12.5), it is more focally expressed in the mesenchyme surrounding the right middle lobe and accessory buds as these lobes form.
T13984 23608-23613 IN denotes While
T13986 23614-23618 NN denotes Fog2
T13987 23619-23629 NN denotes expression
T13985 23630-23632 VBZ denotes is
T13989 23633-23640 JJ denotes diffuse
T13990 23641-23643 IN denotes in
T13991 23644-23647 DT denotes the
T13993 23648-23657 JJ denotes pulmonary
T13992 23658-23668 NN denotes mesenchyme
T13994 23669-23674 IN denotes after
T13996 23675-23680 NN denotes lobar
T13997 23681-23690 NN denotes structure
T13995 23691-23693 VBZ denotes is
T13998 23694-23698 RB denotes well
T13999 23699-23710 VBN denotes established
T14000 23711-23712 -LRB- denotes (
T14001 23712-23717 NN denotes E12.5
T14002 23717-23718 -RRB- denotes )
T14003 23718-23720 , denotes ,
T14004 23720-23722 PRP denotes it
T14005 23723-23725 VBZ denotes is
T14006 23726-23730 RBR denotes more
T14007 23731-23738 RB denotes focally
T13988 23739-23748 VBN denotes expressed
T14008 23749-23751 IN denotes in
T14009 23752-23755 DT denotes the
T14010 23756-23766 NN denotes mesenchyme
T14011 23767-23778 VBG denotes surrounding
T14012 23779-23782 DT denotes the
T14014 23783-23788 JJ denotes right
T14015 23789-23795 JJ denotes middle
T14013 23796-23800 NN denotes lobe
T14016 23801-23804 CC denotes and
T14017 23805-23814 JJ denotes accessory
T14018 23815-23819 NNS denotes buds
T14019 23820-23822 IN denotes as
T14021 23823-23828 DT denotes these
T14022 23829-23834 NNS denotes lobes
T14020 23835-23839 VBP denotes form
T14023 23839-23840 . denotes .
T14024 23840-24000 sentence denotes This matches the phenotype of right middle lobe and accessory lobe loss, and suggests that Fog2 has a specific patterning role in establishment of these lobes.
T14025 23841-23845 DT denotes This
T14026 23846-23853 VBZ denotes matches
T14027 23854-23857 DT denotes the
T14028 23858-23867 NN denotes phenotype
T14029 23868-23870 IN denotes of
T14030 23871-23876 JJ denotes right
T14032 23877-23883 JJ denotes middle
T14031 23884-23888 NN denotes lobe
T14034 23889-23892 CC denotes and
T14035 23893-23902 JJ denotes accessory
T14036 23903-23907 NN denotes lobe
T14033 23908-23912 NN denotes loss
T14037 23912-23914 , denotes ,
T14038 23914-23917 CC denotes and
T14039 23918-23926 VBZ denotes suggests
T14040 23927-23931 IN denotes that
T14042 23932-23936 NN denotes Fog2
T14041 23937-23940 VBZ denotes has
T14043 23941-23942 DT denotes a
T14045 23943-23951 JJ denotes specific
T14046 23952-23962 NN denotes patterning
T14044 23963-23967 NN denotes role
T14047 23968-23970 IN denotes in
T14048 23971-23984 NN denotes establishment
T14049 23985-23987 IN denotes of
T14050 23988-23993 DT denotes these
T14051 23994-23999 NNS denotes lobes
T14052 23999-24000 . denotes .
T14053 24000-24172 sentence denotes It is less clear whether loss of Fog2 results in a global branching defect, as Fog2 lungs appear to have a slight developmental delay, which could result from many causes.
T14054 24001-24003 PRP denotes It
T14055 24004-24006 VBZ denotes is
T14056 24007-24011 RBR denotes less
T14057 24012-24017 JJ denotes clear
T14058 24018-24025 IN denotes whether
T14060 24026-24030 NN denotes loss
T14061 24031-24033 IN denotes of
T14062 24034-24038 NN denotes Fog2
T14059 24039-24046 VBZ denotes results
T14063 24047-24049 IN denotes in
T14064 24050-24051 DT denotes a
T14066 24052-24058 JJ denotes global
T14067 24059-24068 NN denotes branching
T14065 24069-24075 NN denotes defect
T14068 24075-24077 , denotes ,
T14069 24077-24079 IN denotes as
T14071 24080-24084 NN denotes Fog2
T14072 24085-24090 NNS denotes lungs
T14070 24091-24097 VBP denotes appear
T14073 24098-24100 TO denotes to
T14074 24101-24105 VB denotes have
T14075 24106-24107 DT denotes a
T14077 24108-24114 JJ denotes slight
T14078 24115-24128 JJ denotes developmental
T14076 24129-24134 NN denotes delay
T14079 24134-24136 , denotes ,
T14080 24136-24141 WDT denotes which
T14082 24142-24147 MD denotes could
T14081 24148-24154 VB denotes result
T14083 24155-24159 IN denotes from
T14084 24160-24164 JJ denotes many
T14085 24165-24171 NNS denotes causes
T14086 24171-24172 . denotes .
T14087 24172-24335 sentence denotes Cultured Fog2 lungs do develop an intricate branching pattern in the unaffected lobes that appears similar in the pattern to wild-type lungs after 5 d in culture.
T14088 24173-24181 VBN denotes Cultured
T14090 24182-24186 NN denotes Fog2
T14089 24187-24192 NNS denotes lungs
T14092 24193-24195 VBP denotes do
T14091 24196-24203 VB denotes develop
T14093 24204-24206 DT denotes an
T14095 24207-24216 JJ denotes intricate
T14096 24217-24226 NN denotes branching
T14094 24227-24234 NN denotes pattern
T14097 24235-24237 IN denotes in
T14098 24238-24241 DT denotes the
T14100 24242-24252 JJ denotes unaffected
T14099 24253-24258 NNS denotes lobes
T14101 24259-24263 WDT denotes that
T14102 24264-24271 VBZ denotes appears
T14103 24272-24279 JJ denotes similar
T14104 24280-24282 IN denotes in
T14105 24283-24286 DT denotes the
T14106 24287-24294 NN denotes pattern
T14107 24295-24297 IN denotes to
T14108 24298-24302 JJ denotes wild
T14110 24302-24303 HYPH denotes -
T14109 24303-24307 NN denotes type
T14111 24308-24313 NNS denotes lungs
T14112 24314-24319 IN denotes after
T14113 24320-24321 CD denotes 5
T14114 24322-24323 NN denotes d
T14115 24324-24326 IN denotes in
T14116 24327-24334 NN denotes culture
T14117 24334-24335 . denotes .
T14118 24335-24493 sentence denotes In this report, we show that a mutation of Fog2 in the mouse causes the phenotype of abnormal diaphragmatic muscularization and primary pulmonary hypoplasia.
T14119 24336-24338 IN denotes In
T14121 24339-24343 DT denotes this
T14122 24344-24350 NN denotes report
T14123 24350-24352 , denotes ,
T14124 24352-24354 PRP denotes we
T14120 24355-24359 VBP denotes show
T14125 24360-24364 IN denotes that
T14127 24365-24366 DT denotes a
T14128 24367-24375 NN denotes mutation
T14129 24376-24378 IN denotes of
T14130 24379-24383 NN denotes Fog2
T14131 24384-24386 IN denotes in
T14132 24387-24390 DT denotes the
T14133 24391-24396 NN denotes mouse
T14126 24397-24403 VBZ denotes causes
T14134 24404-24407 DT denotes the
T14135 24408-24417 NN denotes phenotype
T14136 24418-24420 IN denotes of
T14137 24421-24429 JJ denotes abnormal
T14139 24430-24443 JJ denotes diaphragmatic
T14138 24444-24459 NN denotes muscularization
T14140 24460-24463 CC denotes and
T14141 24464-24471 JJ denotes primary
T14143 24472-24481 JJ denotes pulmonary
T14142 24482-24492 NN denotes hypoplasia
T14144 24492-24493 . denotes .
T14145 24493-24630 sentence denotes We furthermore demonstrate that a mutation in this gene is associated with a lethal defect in lung and diaphragm development in a child.
T14146 24494-24496 PRP denotes We
T14148 24497-24508 RB denotes furthermore
T14147 24509-24520 VBP denotes demonstrate
T14149 24521-24525 IN denotes that
T14151 24526-24527 DT denotes a
T14152 24528-24536 NN denotes mutation
T14153 24537-24539 IN denotes in
T14154 24540-24544 DT denotes this
T14155 24545-24549 NN denotes gene
T14150 24550-24552 VBZ denotes is
T14156 24553-24563 JJ denotes associated
T14157 24564-24568 IN denotes with
T14158 24569-24570 DT denotes a
T14160 24571-24577 JJ denotes lethal
T14159 24578-24584 NN denotes defect
T14161 24585-24587 IN denotes in
T14162 24588-24592 NN denotes lung
T14164 24593-24596 CC denotes and
T14165 24597-24606 NN denotes diaphragm
T14163 24607-24618 NN denotes development
T14166 24619-24621 IN denotes in
T14167 24622-24623 DT denotes a
T14168 24624-24629 NN denotes child
T14169 24629-24630 . denotes .
T14170 24630-24813 sentence denotes It is notable that, despite extensive analysis of Fog2 biology and the generation of a Fog2 knock-out mouse, its role in diaphragm and lung development was previously not recognized.
T14171 24631-24633 PRP denotes It
T14172 24634-24636 VBZ denotes is
T14173 24637-24644 JJ denotes notable
T14174 24645-24649 IN denotes that
T14176 24649-24651 , denotes ,
T14177 24651-24658 IN denotes despite
T14178 24659-24668 JJ denotes extensive
T14179 24669-24677 NN denotes analysis
T14180 24678-24680 IN denotes of
T14181 24681-24685 NN denotes Fog2
T14182 24686-24693 NN denotes biology
T14183 24694-24697 CC denotes and
T14184 24698-24701 DT denotes the
T14185 24702-24712 NN denotes generation
T14186 24713-24715 IN denotes of
T14187 24716-24717 DT denotes a
T14189 24718-24722 NN denotes Fog2
T14190 24723-24728 VB denotes knock
T14191 24728-24729 HYPH denotes -
T14192 24729-24732 RP denotes out
T14188 24733-24738 NN denotes mouse
T14193 24738-24740 , denotes ,
T14194 24740-24743 PRP$ denotes its
T14195 24744-24748 NN denotes role
T14196 24749-24751 IN denotes in
T14197 24752-24761 NN denotes diaphragm
T14199 24762-24765 CC denotes and
T14200 24766-24770 NN denotes lung
T14198 24771-24782 NN denotes development
T14201 24783-24786 VBD denotes was
T14202 24787-24797 RB denotes previously
T14203 24798-24801 RB denotes not
T14175 24802-24812 VBN denotes recognized
T14204 24812-24813 . denotes .
T14205 24813-25016 sentence denotes It is only as a consequence of phenotype-driven analyses such as those we are pursuing that one has the opportunity to assay all of the potential molecular derangements that may result in human disease.
T14206 24814-24816 PRP denotes It
T14207 24817-24819 VBZ denotes is
T14208 24820-24824 RB denotes only
T14209 24825-24827 IN denotes as
T14210 24828-24829 DT denotes a
T14211 24830-24841 NN denotes consequence
T14212 24842-24844 IN denotes of
T14213 24845-24854 NN denotes phenotype
T14215 24854-24855 HYPH denotes -
T14214 24855-24861 VBN denotes driven
T14216 24862-24870 NNS denotes analyses
T14217 24871-24875 JJ denotes such
T14218 24876-24878 IN denotes as
T14219 24879-24884 DT denotes those
T14220 24885-24887 PRP denotes we
T14222 24888-24891 VBP denotes are
T14221 24892-24900 VBG denotes pursuing
T14223 24901-24905 IN denotes that
T14225 24906-24909 PRP denotes one
T14224 24910-24913 VBZ denotes has
T14226 24914-24917 DT denotes the
T14227 24918-24929 NN denotes opportunity
T14228 24930-24932 TO denotes to
T14229 24933-24938 VB denotes assay
T14230 24939-24942 DT denotes all
T14231 24943-24945 IN denotes of
T14232 24946-24949 DT denotes the
T14234 24950-24959 JJ denotes potential
T14235 24960-24969 JJ denotes molecular
T14233 24970-24982 NNS denotes derangements
T14236 24983-24987 WDT denotes that
T14238 24988-24991 MD denotes may
T14237 24992-24998 VB denotes result
T14239 24999-25001 IN denotes in
T14240 25002-25007 JJ denotes human
T14241 25008-25015 NN denotes disease
T14242 25015-25016 . denotes .
T14396 25311-25318 JJ denotes Genetic
T14397 25319-25326 NN denotes mapping
T14398 25327-25329 IN denotes of
T14399 25330-25333 DT denotes the
T14401 25334-25339 NN denotes mouse
T14400 25340-25348 NN denotes mutation
T14402 25349-25352 NN denotes lil
T14403 25352-25353 . denotes .
T14404 25353-25410 sentence denotes The lil mutation was identified as described in results.
T14405 25354-25357 DT denotes The
T14407 25358-25361 NN denotes lil
T14406 25362-25370 NN denotes mutation
T14409 25371-25374 VBD denotes was
T14408 25375-25385 VBN denotes identified
T14410 25386-25388 IN denotes as
T14411 25389-25398 VBN denotes described
T14412 25399-25401 IN denotes in
T14413 25402-25409 NNS denotes results
T14414 25409-25410 . denotes .
T14415 25410-25547 sentence denotes Wild-type FVB/N and C57BL/6J mice used for genetic crosses were obtained from the Jackson Laboratory (Bar Harbor, Maine, United States).
T14416 25411-25415 JJ denotes Wild
T14418 25415-25416 HYPH denotes -
T14417 25416-25420 NN denotes type
T14420 25421-25424 NN denotes FVB
T14422 25424-25425 HYPH denotes /
T14421 25425-25426 NN denotes N
T14423 25427-25430 CC denotes and
T14424 25431-25436 NN denotes C57BL
T14426 25436-25437 HYPH denotes /
T14425 25437-25439 NN denotes 6J
T14419 25440-25444 NNS denotes mice
T14428 25445-25449 VBN denotes used
T14429 25450-25453 IN denotes for
T14430 25454-25461 JJ denotes genetic
T14431 25462-25469 NNS denotes crosses
T14432 25470-25474 VBD denotes were
T14427 25475-25483 VBN denotes obtained
T14433 25484-25488 IN denotes from
T14434 25489-25492 DT denotes the
T14436 25493-25500 NNP denotes Jackson
T14435 25501-25511 NNP denotes Laboratory
T14437 25512-25513 -LRB- denotes (
T14439 25513-25516 NNP denotes Bar
T14438 25517-25523 NNP denotes Harbor
T14440 25523-25525 , denotes ,
T14441 25525-25530 NNP denotes Maine
T14442 25530-25532 , denotes ,
T14443 25532-25538 NNP denotes United
T14444 25539-25545 NNP denotes States
T14445 25545-25546 -RRB- denotes )
T14446 25546-25547 . denotes .
T14447 25547-25662 sentence denotes Mice carrying a null mutation of Fog2 generated by gene targeting [16] were the generous gift of Dr. Stuart Orkin.
T14448 25548-25552 NNS denotes Mice
T14450 25553-25561 VBG denotes carrying
T14451 25562-25563 DT denotes a
T14453 25564-25568 JJ denotes null
T14452 25569-25577 NN denotes mutation
T14454 25578-25580 IN denotes of
T14455 25581-25585 NN denotes Fog2
T14456 25586-25595 VBN denotes generated
T14457 25596-25598 IN denotes by
T14458 25599-25603 NN denotes gene
T14459 25604-25613 NN denotes targeting
T14460 25614-25615 -LRB- denotes [
T14461 25615-25617 CD denotes 16
T14462 25617-25618 -RRB- denotes ]
T14449 25619-25623 VBD denotes were
T14463 25624-25627 DT denotes the
T14465 25628-25636 JJ denotes generous
T14464 25637-25641 NN denotes gift
T14466 25642-25644 IN denotes of
T14467 25645-25648 NNP denotes Dr.
T14469 25649-25655 NNP denotes Stuart
T14468 25656-25661 NNP denotes Orkin
T14470 25661-25662 . denotes .
T15313 25664-25677 JJ denotes Developmental
T15314 25678-25686 NN denotes analysis
T15315 25687-25689 IN denotes of
T15316 25690-25694 NNS denotes mice
T15317 25694-25695 . denotes .
T15318 25695-25764 sentence denotes Timed pregnancies were set up for collection of E11.5–E17.5 embryos.
T15319 25696-25701 JJ denotes Timed
T15320 25702-25713 NNS denotes pregnancies
T15322 25714-25718 VBD denotes were
T15321 25719-25722 VBN denotes set
T15323 25723-25725 RP denotes up
T15324 25726-25729 IN denotes for
T15325 25730-25740 NN denotes collection
T15326 25741-25743 IN denotes of
T15327 25744-25749 NN denotes E11.5
T15329 25749-25750 SYM denotes –
T15330 25750-25755 NN denotes E17.5
T15328 25756-25763 NNS denotes embryos
T15331 25763-25764 . denotes .
T15332 25764-25842 sentence denotes Embryos were fixed, dehydrated, and embedded in paraffin prior to sectioning.
T15333 25765-25772 NNS denotes Embryos
T15335 25773-25777 VBD denotes were
T15334 25778-25783 VBN denotes fixed
T15336 25783-25785 , denotes ,
T15337 25785-25795 VBN denotes dehydrated
T15338 25795-25797 , denotes ,
T15339 25797-25800 CC denotes and
T15340 25801-25809 VBN denotes embedded
T15341 25810-25812 IN denotes in
T15342 25813-25821 NN denotes paraffin
T15343 25822-25827 JJ denotes prior
T15344 25828-25830 IN denotes to
T15345 25831-25841 NN denotes sectioning
T15346 25841-25842 . denotes .
T15347 25842-25969 sentence denotes In older embryos, a median sternotomy was performed under microscopic guidance, and diaphragm, lungs, and heart were examined.
T15348 25843-25845 IN denotes In
T15350 25846-25851 JJR denotes older
T15351 25852-25859 NNS denotes embryos
T15352 25859-25861 , denotes ,
T15353 25861-25862 DT denotes a
T15355 25863-25869 JJ denotes median
T15354 25870-25880 NN denotes sternotomy
T15356 25881-25884 VBD denotes was
T15349 25885-25894 VBN denotes performed
T15357 25895-25900 IN denotes under
T15358 25901-25912 JJ denotes microscopic
T15359 25913-25921 NN denotes guidance
T15360 25921-25923 , denotes ,
T15361 25923-25926 CC denotes and
T15362 25927-25936 NN denotes diaphragm
T15364 25936-25938 , denotes ,
T15365 25938-25943 NNS denotes lungs
T15366 25943-25945 , denotes ,
T15367 25945-25948 CC denotes and
T15368 25949-25954 NN denotes heart
T15369 25955-25959 VBD denotes were
T15363 25960-25968 VBN denotes examined
T15370 25968-25969 . denotes .
T15371 25969-26031 sentence denotes The lungs and tracheobronchial tree were removed and weighed.
T15372 25970-25973 DT denotes The
T15373 25974-25979 NNS denotes lungs
T15375 25980-25983 CC denotes and
T15376 25984-26000 JJ denotes tracheobronchial
T15377 26001-26005 NN denotes tree
T15378 26006-26010 VBD denotes were
T15374 26011-26018 VBN denotes removed
T15379 26019-26022 CC denotes and
T15380 26023-26030 VBN denotes weighed
T15381 26030-26031 . denotes .
T15382 26031-26119 sentence denotes Whole diaphragms were isolated from fixed thoracic tissue from E15.5 and E17.5 embryos.
T15383 26032-26037 JJ denotes Whole
T15384 26038-26048 NNS denotes diaphragms
T15386 26049-26053 VBD denotes were
T15385 26054-26062 VBN denotes isolated
T15387 26063-26067 IN denotes from
T15388 26068-26073 VBN denotes fixed
T15390 26074-26082 JJ denotes thoracic
T15389 26083-26089 NN denotes tissue
T15391 26090-26094 IN denotes from
T15392 26095-26100 NN denotes E15.5
T15394 26101-26104 CC denotes and
T15395 26105-26110 NN denotes E17.5
T15393 26111-26118 NNS denotes embryos
T15396 26118-26119 . denotes .
T15397 26119-26558 sentence denotes For lung explant culture, lungs were dissected from fresh embryos at E11.5 and E12.5 and placed on porous 24-mm (0.4-μ) polyester membranes floated in wells containing 2 ml of Dulbecco's modified Eagle's medium, nutrient mixture F-12 (11039–021, Gibco, San Diego, California, United States), supplemented with 10% fetal bovine serum, 0.3 mg/ml L-glutamine, 100 units/ml penicillin, 100 mcg/ml streptomycin, and 0.25 mcg/ml amphotericin B.
T15398 26120-26123 IN denotes For
T15400 26124-26128 NN denotes lung
T15402 26129-26136 NN denotes explant
T15401 26137-26144 NN denotes culture
T15403 26144-26146 , denotes ,
T15404 26146-26151 NNS denotes lungs
T15405 26152-26156 VBD denotes were
T15399 26157-26166 VBN denotes dissected
T15406 26167-26171 IN denotes from
T15407 26172-26177 JJ denotes fresh
T15408 26178-26185 NNS denotes embryos
T15409 26186-26188 IN denotes at
T15410 26189-26194 NN denotes E11.5
T15411 26195-26198 CC denotes and
T15412 26199-26204 NN denotes E12.5
T15413 26205-26208 CC denotes and
T15414 26209-26215 VBN denotes placed
T15415 26216-26218 IN denotes on
T15416 26219-26225 JJ denotes porous
T15418 26226-26228 CD denotes 24
T15420 26228-26229 HYPH denotes -
T15419 26229-26231 NN denotes mm
T15421 26232-26233 -LRB- denotes (
T15423 26233-26236 CD denotes 0.4
T15424 26236-26237 HYPH denotes -
T15422 26237-26238 NN denotes μ
T15425 26238-26239 -RRB- denotes )
T15426 26240-26249 NN denotes polyester
T15417 26250-26259 NNS denotes membranes
T15427 26260-26267 VBN denotes floated
T15428 26268-26270 IN denotes in
T15429 26271-26276 NNS denotes wells
T15430 26277-26287 VBG denotes containing
T15431 26288-26289 CD denotes 2
T15432 26290-26292 NN denotes ml
T15433 26293-26295 IN denotes of
T15434 26296-26304 NNP denotes Dulbecco
T15436 26304-26306 POS denotes 's
T15437 26307-26315 VBN denotes modified
T15438 26316-26321 NNP denotes Eagle
T15439 26321-26323 POS denotes 's
T15435 26324-26330 NN denotes medium
T15440 26330-26332 , denotes ,
T15441 26332-26340 NN denotes nutrient
T15442 26341-26348 NN denotes mixture
T15443 26349-26350 NN denotes F
T15444 26350-26351 HYPH denotes -
T15445 26351-26353 CD denotes 12
T15446 26354-26355 -LRB- denotes (
T15448 26355-26360 CD denotes 11039
T15450 26360-26361 HYPH denotes –
T15449 26361-26364 CD denotes 021
T15451 26364-26366 , denotes ,
T15447 26366-26371 NNP denotes Gibco
T15452 26371-26373 , denotes ,
T15453 26373-26376 NNP denotes San
T15454 26377-26382 NNP denotes Diego
T15455 26382-26384 , denotes ,
T15456 26384-26394 NNP denotes California
T15457 26394-26396 , denotes ,
T15458 26396-26402 NNP denotes United
T15459 26403-26409 NNP denotes States
T15460 26409-26410 -RRB- denotes )
T15461 26410-26412 , denotes ,
T15462 26412-26424 VBN denotes supplemented
T15463 26425-26429 IN denotes with
T15464 26430-26432 CD denotes 10
T15465 26432-26433 NN denotes %
T15467 26434-26439 JJ denotes fetal
T15468 26440-26446 JJ denotes bovine
T15466 26447-26452 NN denotes serum
T15469 26452-26454 , denotes ,
T15470 26454-26457 CD denotes 0.3
T15471 26458-26460 NN denotes mg
T15473 26460-26461 SYM denotes /
T15474 26461-26463 NN denotes ml
T15475 26464-26465 NN denotes L
T15476 26465-26466 HYPH denotes -
T15472 26466-26475 NN denotes glutamine
T15477 26475-26477 , denotes ,
T15478 26477-26480 CD denotes 100
T15479 26481-26486 NNS denotes units
T15481 26486-26487 SYM denotes /
T15482 26487-26489 NN denotes ml
T15480 26490-26500 NN denotes penicillin
T15483 26500-26502 , denotes ,
T15484 26502-26505 CD denotes 100
T15485 26506-26509 NN denotes mcg
T15487 26509-26510 SYM denotes /
T15488 26510-26512 NN denotes ml
T15486 26513-26525 NN denotes streptomycin
T15489 26525-26527 , denotes ,
T15490 26527-26530 CC denotes and
T15491 26531-26535 CD denotes 0.25
T15492 26536-26539 NN denotes mcg
T15494 26539-26540 SYM denotes /
T15495 26540-26542 NN denotes ml
T15496 26543-26555 NN denotes amphotericin
T15493 26556-26557 NN denotes B
T15497 26557-26558 . denotes .
T15498 26558-26628 sentence denotes Lung explants were cultured at 37 °C in 95% air/5% CO2 for up to 5 d.
T15499 26559-26563 NN denotes Lung
T15500 26564-26572 NNS denotes explants
T15502 26573-26577 VBD denotes were
T15501 26578-26586 VBN denotes cultured
T15503 26587-26589 IN denotes at
T15504 26590-26592 CD denotes 37
T15505 26593-26595 NN denotes °C
T15506 26596-26598 IN denotes in
T15507 26599-26601 CD denotes 95
T15508 26601-26602 NN denotes %
T15509 26603-26606 NN denotes air
T15510 26606-26607 HYPH denotes /
T15511 26607-26608 CD denotes 5
T15512 26608-26609 NN denotes %
T15513 26610-26613 NN denotes CO2
T15514 26614-26617 IN denotes for
T15515 26618-26620 RB denotes up
T15517 26621-26623 IN denotes to
T15516 26624-26625 CD denotes 5
T15518 26626-26627 NN denotes d
T15519 26627-26628 . denotes .
T15520 26628-26764 sentence denotes They were photographed daily with a dissecting microscope (MZ12.5, Leica, Wetzlar, Germany) equipped with a Leica DC500 digital camera.
T15521 26629-26633 PRP denotes They
T15523 26634-26638 VBD denotes were
T15522 26639-26651 VBN denotes photographed
T15524 26652-26657 RB denotes daily
T15525 26658-26662 IN denotes with
T15526 26663-26664 DT denotes a
T15528 26665-26675 VBG denotes dissecting
T15527 26676-26686 NN denotes microscope
T15529 26687-26688 -LRB- denotes (
T15530 26688-26694 NN denotes MZ12.5
T15531 26694-26696 , denotes ,
T15532 26696-26701 NNP denotes Leica
T15533 26701-26703 , denotes ,
T15534 26703-26710 NNP denotes Wetzlar
T15535 26710-26712 , denotes ,
T15536 26712-26719 NNP denotes Germany
T15537 26719-26720 -RRB- denotes )
T15538 26721-26729 VBN denotes equipped
T15539 26730-26734 IN denotes with
T15540 26735-26736 DT denotes a
T15542 26737-26742 NNP denotes Leica
T15543 26743-26748 NN denotes DC500
T15544 26749-26756 JJ denotes digital
T15541 26757-26763 NN denotes camera
T15545 26763-26764 . denotes .
T15546 26764-26867 sentence denotes Transgenic mice carrying the lacZ gene driven by the Fog2 promoter have been developed by S. Tevosian.
T15547 26765-26775 JJ denotes Transgenic
T15548 26776-26780 NNS denotes mice
T15550 26781-26789 VBG denotes carrying
T15551 26790-26793 DT denotes the
T15553 26794-26798 NN denotes lacZ
T15552 26799-26803 NN denotes gene
T15554 26804-26810 VBN denotes driven
T15555 26811-26813 IN denotes by
T15556 26814-26817 DT denotes the
T15558 26818-26822 NN denotes Fog2
T15557 26823-26831 NN denotes promoter
T15559 26832-26836 VBP denotes have
T15560 26837-26841 VBN denotes been
T15549 26842-26851 VBN denotes developed
T15561 26852-26854 IN denotes by
T15562 26855-26857 NNP denotes S.
T15563 26858-26866 NNP denotes Tevosian
T15564 26866-26867 . denotes .
T15565 26867-27067 sentence denotes In these animals, the lacZ gene is incorporated (“knocked-in”) into the Fog2 locus to allow β-galactosidase expression as a fusion protein in frame with the first 235 amino acids of the FOG2 protein.
T15566 26868-26870 IN denotes In
T15568 26871-26876 DT denotes these
T15569 26877-26884 NNS denotes animals
T15570 26884-26886 , denotes ,
T15571 26886-26889 DT denotes the
T15573 26890-26894 NN denotes lacZ
T15572 26895-26899 NN denotes gene
T15574 26900-26902 VBZ denotes is
T15567 26903-26915 VBN denotes incorporated
T15575 26916-26917 -LRB- denotes (
T15577 26917-26918 `` denotes “
T15576 26918-26925 VBN denotes knocked
T15578 26925-26926 HYPH denotes -
T15579 26926-26928 RP denotes in
T15580 26928-26929 '' denotes ”
T15581 26929-26930 -RRB- denotes )
T15582 26931-26935 IN denotes into
T15583 26936-26939 DT denotes the
T15585 26940-26944 NN denotes Fog2
T15584 26945-26950 NN denotes locus
T15586 26951-26953 TO denotes to
T15587 26954-26959 VB denotes allow
T15588 26960-26961 NN denotes β
T15590 26961-26962 HYPH denotes -
T15589 26962-26975 NN denotes galactosidase
T15591 26976-26986 NN denotes expression
T15592 26987-26989 IN denotes as
T15593 26990-26991 DT denotes a
T15595 26992-26998 NN denotes fusion
T15594 26999-27006 NN denotes protein
T15596 27007-27009 IN denotes in
T15597 27010-27015 NN denotes frame
T15598 27016-27020 IN denotes with
T15599 27021-27024 DT denotes the
T15601 27025-27030 JJ denotes first
T15602 27031-27034 CD denotes 235
T15603 27035-27040 NN denotes amino
T15600 27041-27046 NNS denotes acids
T15604 27047-27049 IN denotes of
T15605 27050-27053 DT denotes the
T15607 27054-27058 NN denotes FOG2
T15606 27059-27066 NN denotes protein
T15608 27066-27067 . denotes .
T15609 27067-27126 sentence denotes The Fog2-lacZ module is followed by an ires-eGFP cassette.
T15610 27068-27071 DT denotes The
T15612 27072-27076 NN denotes Fog2
T15614 27076-27077 HYPH denotes -
T15613 27077-27081 NN denotes lacZ
T15611 27082-27088 NN denotes module
T15616 27089-27091 VBZ denotes is
T15615 27092-27100 VBN denotes followed
T15617 27101-27103 IN denotes by
T15618 27104-27106 DT denotes an
T15620 27107-27111 NN denotes ires
T15622 27111-27112 HYPH denotes -
T15621 27112-27116 NN denotes eGFP
T15619 27117-27125 NN denotes cassette
T15623 27125-27126 . denotes .
T15624 27126-27167 sentence denotes This creates a null allele of Fog2 gene.
T15625 27127-27131 DT denotes This
T15626 27132-27139 VBZ denotes creates
T15627 27140-27141 DT denotes a
T15629 27142-27146 JJ denotes null
T15628 27147-27153 NN denotes allele
T15630 27154-27156 IN denotes of
T15631 27157-27161 NN denotes Fog2
T15632 27162-27166 NN denotes gene
T15633 27166-27167 . denotes .
T15634 27167-27263 sentence denotes The Fog2-LacZ-eGFP construct was linearized with KspI and electroporated into the CJ7 ES cells.
T15635 27168-27171 DT denotes The
T15637 27172-27176 NN denotes Fog2
T15639 27176-27177 HYPH denotes -
T15640 27177-27181 NN denotes LacZ
T15641 27181-27182 HYPH denotes -
T15638 27182-27186 NN denotes eGFP
T15636 27187-27196 NN denotes construct
T15643 27197-27200 VBD denotes was
T15642 27201-27211 VBN denotes linearized
T15644 27212-27216 IN denotes with
T15645 27217-27221 NN denotes KspI
T15646 27222-27225 CC denotes and
T15647 27226-27240 VBN denotes electroporated
T15648 27241-27245 IN denotes into
T15649 27246-27249 DT denotes the
T15651 27250-27253 NN denotes CJ7
T15652 27254-27256 NN denotes ES
T15650 27257-27262 NNS denotes cells
T15653 27262-27263 . denotes .
T15654 27263-27375 sentence denotes The correctly targeted clone was selected by the Southern blot analysis and injected into C57BL/6J blastocysts.
T15655 27264-27267 DT denotes The
T15657 27268-27277 RB denotes correctly
T15658 27278-27286 VBN denotes targeted
T15656 27287-27292 NN denotes clone
T15660 27293-27296 VBD denotes was
T15659 27297-27305 VBN denotes selected
T15661 27306-27308 IN denotes by
T15662 27309-27312 DT denotes the
T15664 27313-27321 NNP denotes Southern
T15665 27322-27326 NN denotes blot
T15663 27327-27335 NN denotes analysis
T15666 27336-27339 CC denotes and
T15667 27340-27348 VBN denotes injected
T15668 27349-27353 IN denotes into
T15669 27354-27359 NN denotes C57BL
T15671 27359-27360 HYPH denotes /
T15670 27360-27362 NN denotes 6J
T15672 27363-27374 NNS denotes blastocysts
T15673 27374-27375 . denotes .
T15674 27375-27452 sentence denotes Fog2-lacZ-eGFP animals were maintained on the mixed C57BL/6J/129 background.
T15675 27376-27380 NN denotes Fog2
T15677 27380-27381 HYPH denotes -
T15678 27381-27385 NN denotes lacZ
T15679 27385-27386 HYPH denotes -
T15676 27386-27390 NN denotes eGFP
T15680 27391-27398 NNS denotes animals
T15682 27399-27403 VBD denotes were
T15681 27404-27414 VBN denotes maintained
T15683 27415-27417 IN denotes on
T15684 27418-27421 DT denotes the
T15686 27422-27427 JJ denotes mixed
T15687 27428-27433 NN denotes C57BL
T15689 27433-27434 HYPH denotes /
T15688 27434-27436 NN denotes 6J
T15690 27436-27437 HYPH denotes /
T15691 27437-27440 CD denotes 129
T15685 27441-27451 NN denotes background
T15692 27451-27452 . denotes .
T15693 27452-27595 sentence denotes lacZ Expression in whole dissected embryonic lungs was analyzed by staining for β-galactosidase activity with X-gal after fixation for 30 min.
T15694 27453-27457 NN denotes lacZ
T15695 27458-27468 NN denotes Expression
T15697 27469-27471 IN denotes in
T15698 27472-27477 JJ denotes whole
T15700 27478-27487 VBN denotes dissected
T15701 27488-27497 JJ denotes embryonic
T15699 27498-27503 NNS denotes lungs
T15702 27504-27507 VBD denotes was
T15696 27508-27516 VBN denotes analyzed
T15703 27517-27519 IN denotes by
T15704 27520-27528 VBG denotes staining
T15705 27529-27532 IN denotes for
T15706 27533-27534 NN denotes β
T15708 27534-27535 HYPH denotes -
T15707 27535-27548 NN denotes galactosidase
T15709 27549-27557 NN denotes activity
T15710 27558-27562 IN denotes with
T15711 27563-27564 NN denotes X
T15713 27564-27565 HYPH denotes -
T15712 27565-27568 NN denotes gal
T15714 27569-27574 IN denotes after
T15715 27575-27583 NN denotes fixation
T15716 27584-27587 IN denotes for
T15717 27588-27590 CD denotes 30
T15718 27591-27594 NN denotes min
T15719 27594-27595 . denotes .
T16000 27597-27599 NN denotes RT
T16002 27599-27600 HYPH denotes -
T16001 27600-27603 NN denotes PCR
T16003 27604-27607 CC denotes and
T16004 27608-27616 NN denotes sequence
T16005 27617-27625 NN denotes analysis
T16006 27626-27628 IN denotes in
T16007 27629-27632 DT denotes the
T16008 27633-27638 NN denotes mouse
T16009 27638-27639 . denotes .
T16010 27639-27712 sentence denotes RNA was extracted by standard techniques from thoracic embryonic tissue.
T16011 27640-27643 NN denotes RNA
T16013 27644-27647 VBD denotes was
T16012 27648-27657 VBN denotes extracted
T16014 27658-27660 IN denotes by
T16015 27661-27669 JJ denotes standard
T16016 27670-27680 NNS denotes techniques
T16017 27681-27685 IN denotes from
T16018 27686-27694 JJ denotes thoracic
T16020 27695-27704 JJ denotes embryonic
T16019 27705-27711 NN denotes tissue
T16021 27711-27712 . denotes .
T16022 27712-27788 sentence denotes RT-PCR was performed using six primer sets designed to cover the Fog2 gene.
T16023 27713-27715 NN denotes RT
T16025 27715-27716 HYPH denotes -
T16024 27716-27719 NN denotes PCR
T16027 27720-27723 VBD denotes was
T16026 27724-27733 VBN denotes performed
T16028 27734-27739 VBG denotes using
T16029 27740-27743 CD denotes six
T16031 27744-27750 NN denotes primer
T16030 27751-27755 NNS denotes sets
T16032 27756-27764 VBN denotes designed
T16033 27765-27767 TO denotes to
T16034 27768-27773 VB denotes cover
T16035 27774-27777 DT denotes the
T16037 27778-27782 NN denotes Fog2
T16036 27783-27787 NN denotes gene
T16038 27787-27788 . denotes .
T16039 27788-27987 sentence denotes RT-PCR was repeated with radiolabeled primers to amplify an abnormally spliced region of the gene (Table S1), and the product was run on a denaturing sequencing gel according to standard techniques.
T16040 27789-27791 NN denotes RT
T16042 27791-27792 HYPH denotes -
T16041 27792-27795 NN denotes PCR
T16044 27796-27799 VBD denotes was
T16043 27800-27808 VBN denotes repeated
T16045 27809-27813 IN denotes with
T16046 27814-27826 VBN denotes radiolabeled
T16047 27827-27834 NNS denotes primers
T16048 27835-27837 TO denotes to
T16049 27838-27845 VB denotes amplify
T16050 27846-27848 DT denotes an
T16052 27849-27859 RB denotes abnormally
T16053 27860-27867 VBN denotes spliced
T16051 27868-27874 NN denotes region
T16054 27875-27877 IN denotes of
T16055 27878-27881 DT denotes the
T16056 27882-27886 NN denotes gene
T16057 27887-27888 -LRB- denotes (
T16059 27888-27893 NN denotes Table
T16058 27894-27896 NN denotes S1
T16060 27896-27897 -RRB- denotes )
T16061 27897-27899 , denotes ,
T16062 27899-27902 CC denotes and
T16063 27903-27906 DT denotes the
T16064 27907-27914 NN denotes product
T16066 27915-27918 VBD denotes was
T16065 27919-27922 VBN denotes run
T16067 27923-27925 IN denotes on
T16068 27926-27927 DT denotes a
T16070 27928-27938 VBG denotes denaturing
T16071 27939-27949 NN denotes sequencing
T16069 27950-27953 NN denotes gel
T16072 27954-27963 VBG denotes according
T16073 27964-27966 IN denotes to
T16074 27967-27975 JJ denotes standard
T16075 27976-27986 NNS denotes techniques
T16076 27986-27987 . denotes .
T16077 27987-28155 sentence denotes The RT-PCR product was cloned into pCR2.1 vector using TOPO TA Cloning Kit (Invitrogen, Carlsbad, California, United States) and sequenced using gene-specific primers.
T16078 27988-27991 DT denotes The
T16080 27992-27994 NN denotes RT
T16082 27994-27995 HYPH denotes -
T16081 27995-27998 NN denotes PCR
T16079 27999-28006 NN denotes product
T16084 28007-28010 VBD denotes was
T16083 28011-28017 VBN denotes cloned
T16085 28018-28022 IN denotes into
T16086 28023-28029 NN denotes pCR2.1
T16087 28030-28036 NN denotes vector
T16088 28037-28042 VBG denotes using
T16089 28043-28047 NN denotes TOPO
T16091 28048-28050 NN denotes TA
T16092 28051-28058 NNP denotes Cloning
T16090 28059-28062 NNP denotes Kit
T16093 28063-28064 -LRB- denotes (
T16094 28064-28074 NNP denotes Invitrogen
T16095 28074-28076 , denotes ,
T16096 28076-28084 NNP denotes Carlsbad
T16097 28084-28086 , denotes ,
T16098 28086-28096 NNP denotes California
T16099 28096-28098 , denotes ,
T16100 28098-28104 NNP denotes United
T16101 28105-28111 NNP denotes States
T16102 28111-28112 -RRB- denotes )
T16103 28113-28116 CC denotes and
T16104 28117-28126 VBN denotes sequenced
T16105 28127-28132 VBG denotes using
T16106 28133-28137 NN denotes gene
T16108 28137-28138 HYPH denotes -
T16107 28138-28146 JJ denotes specific
T16109 28147-28154 NNS denotes primers
T16110 28154-28155 . denotes .
T16111 28155-28253 sentence denotes Sequence analysis was done using Sequencher 4.1 (Gene Codes, Ann Arbor, Michigan, United States).
T16112 28156-28164 NN denotes Sequence
T16113 28165-28173 NN denotes analysis
T16115 28174-28177 VBD denotes was
T16114 28178-28182 VBN denotes done
T16116 28183-28188 VBG denotes using
T16117 28189-28199 NNP denotes Sequencher
T16118 28200-28203 CD denotes 4.1
T16119 28204-28205 -LRB- denotes (
T16121 28205-28209 NNP denotes Gene
T16120 28210-28215 NNPS denotes Codes
T16122 28215-28217 , denotes ,
T16123 28217-28220 NNP denotes Ann
T16124 28221-28226 NNP denotes Arbor
T16125 28226-28228 , denotes ,
T16126 28228-28236 NNP denotes Michigan
T16127 28236-28238 , denotes ,
T16128 28238-28244 NNP denotes United
T16129 28245-28251 NNP denotes States
T16130 28251-28252 -RRB- denotes )
T16131 28252-28253 . denotes .
T16289 28255-28257 FW denotes In
T16290 28258-28262 FW denotes situ
T16291 28263-28276 NN denotes hybridization
T16292 28276-28277 . denotes .
T16293 28277-28411 sentence denotes After dehydration and embedding in paraffin wax, 10-μ sections were subjected to radioactive in situ hybridization as described [53].
T16294 28278-28283 IN denotes After
T16296 28284-28295 NN denotes dehydration
T16297 28296-28299 CC denotes and
T16298 28300-28309 NN denotes embedding
T16299 28310-28312 IN denotes in
T16300 28313-28321 NN denotes paraffin
T16301 28322-28325 NN denotes wax
T16302 28325-28327 , denotes ,
T16303 28327-28329 CD denotes 10
T16305 28329-28330 HYPH denotes -
T16304 28330-28331 NN denotes μ
T16306 28332-28340 NNS denotes sections
T16307 28341-28345 VBD denotes were
T16295 28346-28355 VBN denotes subjected
T16308 28356-28358 IN denotes to
T16309 28359-28370 JJ denotes radioactive
T16311 28371-28373 FW denotes in
T16312 28374-28378 FW denotes situ
T16310 28379-28392 NN denotes hybridization
T16313 28393-28395 IN denotes as
T16314 28396-28405 VBN denotes described
T16315 28406-28407 -LRB- denotes [
T16316 28407-28409 CD denotes 53
T16317 28409-28410 -RRB- denotes ]
T16318 28410-28411 . denotes .
T16319 28411-28541 sentence denotes Probes labeled with 35S were prepared by run-off transcription of linearized plasmid templates and hybridized to tissue sections.
T16320 28412-28418 NNS denotes Probes
T16322 28419-28426 VBN denotes labeled
T16323 28427-28431 IN denotes with
T16324 28432-28435 NN denotes 35S
T16325 28436-28440 VBD denotes were
T16321 28441-28449 VBN denotes prepared
T16326 28450-28452 IN denotes by
T16327 28453-28456 VB denotes run
T16329 28456-28457 HYPH denotes -
T16330 28457-28460 RP denotes off
T16328 28461-28474 NN denotes transcription
T16331 28475-28477 IN denotes of
T16332 28478-28488 VBN denotes linearized
T16334 28489-28496 NN denotes plasmid
T16333 28497-28506 NNS denotes templates
T16335 28507-28510 CC denotes and
T16336 28511-28521 VBN denotes hybridized
T16337 28522-28524 IN denotes to
T16338 28525-28531 NN denotes tissue
T16339 28532-28540 NNS denotes sections
T16340 28540-28541 . denotes .
T16341 28541-28654 sentence denotes Nuclei were counterstained with Hoescht 33258, and signal was imaged using fluorescent and darkfield microscopy.
T16342 28542-28548 NNS denotes Nuclei
T16344 28549-28553 VBD denotes were
T16343 28554-28568 VBN denotes counterstained
T16345 28569-28573 IN denotes with
T16346 28574-28581 NNP denotes Hoescht
T16347 28582-28587 CD denotes 33258
T16348 28587-28589 , denotes ,
T16349 28589-28592 CC denotes and
T16350 28593-28599 NN denotes signal
T16352 28600-28603 VBD denotes was
T16351 28604-28610 VBN denotes imaged
T16353 28611-28616 VBG denotes using
T16354 28617-28628 JJ denotes fluorescent
T16356 28629-28632 CC denotes and
T16357 28633-28642 NN denotes darkfield
T16355 28643-28653 NN denotes microscopy
T16358 28653-28654 . denotes .
T16688 28656-28661 JJ denotes Human
T16690 28662-28665 NN denotes DNA
T16689 28666-28676 NN denotes extraction
T16691 28677-28680 CC denotes and
T16692 28681-28689 NN denotes sequence
T16693 28690-28698 NN denotes analysis
T16694 28698-28699 . denotes .
T16695 28699-28826 sentence denotes DNA was isolated from paraffin blocks by phenol-chloroform extraction [54,55], and from frozen tissues by standard techniques.
T16696 28700-28703 NN denotes DNA
T16698 28704-28707 VBD denotes was
T16697 28708-28716 VBN denotes isolated
T16699 28717-28721 IN denotes from
T16700 28722-28730 NN denotes paraffin
T16701 28731-28737 NNS denotes blocks
T16702 28738-28740 IN denotes by
T16703 28741-28747 NN denotes phenol
T16705 28747-28748 HYPH denotes -
T16704 28748-28758 NN denotes chloroform
T16706 28759-28769 NN denotes extraction
T16707 28770-28771 -LRB- denotes [
T16709 28771-28773 CD denotes 54
T16710 28773-28774 , denotes ,
T16708 28774-28776 CD denotes 55
T16711 28776-28777 -RRB- denotes ]
T16712 28777-28779 , denotes ,
T16713 28779-28782 CC denotes and
T16714 28783-28787 IN denotes from
T16715 28788-28794 VBN denotes frozen
T16716 28795-28802 NNS denotes tissues
T16717 28803-28805 IN denotes by
T16718 28806-28814 JJ denotes standard
T16719 28815-28825 NNS denotes techniques
T16720 28825-28826 . denotes .
T16721 28826-28934 sentence denotes Primers were designed to amplify FOG2 coding exons plus 50 bp of flanking upstream and downstream sequence.
T16722 28827-28834 NNS denotes Primers
T16724 28835-28839 VBD denotes were
T16723 28840-28848 VBN denotes designed
T16725 28849-28851 TO denotes to
T16726 28852-28859 VB denotes amplify
T16727 28860-28864 NN denotes FOG2
T16729 28865-28871 NN denotes coding
T16728 28872-28877 NNS denotes exons
T16730 28878-28882 CC denotes plus
T16731 28883-28885 CD denotes 50
T16732 28886-28888 NN denotes bp
T16733 28889-28891 IN denotes of
T16734 28892-28900 VBG denotes flanking
T16736 28901-28909 JJ denotes upstream
T16737 28910-28913 CC denotes and
T16738 28914-28924 JJ denotes downstream
T16735 28925-28933 NN denotes sequence
T16739 28933-28934 . denotes .
T16740 28934-29003 sentence denotes PCR amplification and sequencing were performed by standard methods.
T16741 28935-28938 NN denotes PCR
T16742 28939-28952 NN denotes amplification
T16744 28953-28956 CC denotes and
T16745 28957-28967 NN denotes sequencing
T16746 28968-28972 VBD denotes were
T16743 28973-28982 VBN denotes performed
T16747 28983-28985 IN denotes by
T16748 28986-28994 JJ denotes standard
T16749 28995-29002 NNS denotes methods
T16750 29002-29003 . denotes .
T16751 29003-29049 sentence denotes Primer sequences used are listed in Table S2.
T16752 29004-29010 NN denotes Primer
T16753 29011-29020 NNS denotes sequences
T16755 29021-29025 VBN denotes used
T16756 29026-29029 VBP denotes are
T16754 29030-29036 VBN denotes listed
T16757 29037-29039 IN denotes in
T16758 29040-29045 NN denotes Table
T16759 29046-29048 NN denotes S2
T16760 29048-29049 . denotes .
T16761 29049-29110 sentence denotes Sequence analysis was done with Sequencher 4.1 (Gene Codes).
T16762 29050-29058 NN denotes Sequence
T16763 29059-29067 NN denotes analysis
T16765 29068-29071 VBD denotes was
T16764 29072-29076 VBN denotes done
T16766 29077-29081 IN denotes with
T16767 29082-29092 NNP denotes Sequencher
T16768 29093-29096 CD denotes 4.1
T16769 29097-29098 -LRB- denotes (
T16771 29098-29102 NNP denotes Gene
T16770 29103-29108 NNPS denotes Codes
T16772 29108-29109 -RRB- denotes )
T16773 29109-29110 . denotes .
T16774 29110-29194 sentence denotes DNA from the parents of one autopsy patient was extracted from fresh blood samples.
T16775 29111-29114 NN denotes DNA
T16777 29115-29119 IN denotes from
T16778 29120-29123 DT denotes the
T16779 29124-29131 NNS denotes parents
T16780 29132-29134 IN denotes of
T16781 29135-29138 CD denotes one
T16783 29139-29146 NN denotes autopsy
T16782 29147-29154 NN denotes patient
T16784 29155-29158 VBD denotes was
T16776 29159-29168 VBN denotes extracted
T16785 29169-29173 IN denotes from
T16786 29174-29179 JJ denotes fresh
T16788 29180-29185 NN denotes blood
T16787 29186-29193 NNS denotes samples
T16789 29193-29194 . denotes .
T16790 29194-29324 sentence denotes A second set of blood samples was sent to an outside CLIA-certified laboratory for DNA extraction, PCR, sequencing, and analysis.
T16791 29195-29196 DT denotes A
T16793 29197-29203 JJ denotes second
T16792 29204-29207 NN denotes set
T16795 29208-29210 IN denotes of
T16796 29211-29216 NN denotes blood
T16797 29217-29224 NNS denotes samples
T16798 29225-29228 VBD denotes was
T16794 29229-29233 VBN denotes sent
T16799 29234-29236 IN denotes to
T16800 29237-29239 DT denotes an
T16802 29240-29247 JJ denotes outside
T16803 29248-29252 NN denotes CLIA
T16805 29252-29253 HYPH denotes -
T16804 29253-29262 VBN denotes certified
T16801 29263-29273 NN denotes laboratory
T16806 29274-29277 IN denotes for
T16807 29278-29281 NN denotes DNA
T16808 29282-29292 NN denotes extraction
T16809 29292-29294 , denotes ,
T16810 29294-29297 NN denotes PCR
T16811 29297-29299 , denotes ,
T16812 29299-29309 NN denotes sequencing
T16813 29309-29311 , denotes ,
T16814 29311-29314 CC denotes and
T16815 29315-29323 NN denotes analysis
T16816 29323-29324 . denotes .
T16817 29324-29417 sentence denotes Paternity testing was performed by the outside laboratory using a standard panel of markers.
T16818 29325-29334 NN denotes Paternity
T16819 29335-29342 NN denotes testing
T16821 29343-29346 VBD denotes was
T16820 29347-29356 VBN denotes performed
T16822 29357-29359 IN denotes by
T16823 29360-29363 DT denotes the
T16825 29364-29371 JJ denotes outside
T16824 29372-29382 NN denotes laboratory
T16826 29383-29388 VBG denotes using
T16827 29389-29390 DT denotes a
T16829 29391-29399 JJ denotes standard
T16828 29400-29405 NN denotes panel
T16830 29406-29408 IN denotes of
T16831 29409-29416 NNS denotes markers
T16832 29416-29417 . denotes .
T16833 29417-29565 sentence denotes SNP genotyping was done using Harvard Partners Center for Genetics and Genomics genotyping core facility (Cambridge, Massachusetts, United States).
T16834 29418-29421 NN denotes SNP
T16835 29422-29432 NN denotes genotyping
T16837 29433-29436 VBD denotes was
T16836 29437-29441 VBN denotes done
T16838 29442-29447 VBG denotes using
T16839 29448-29455 NNP denotes Harvard
T16841 29456-29464 NNPS denotes Partners
T16840 29465-29471 NNP denotes Center
T16843 29472-29475 IN denotes for
T16844 29476-29484 NNP denotes Genetics
T16845 29485-29488 CC denotes and
T16846 29489-29497 NNP denotes Genomics
T16847 29498-29508 NN denotes genotyping
T16848 29509-29513 NN denotes core
T16842 29514-29522 NN denotes facility
T16849 29523-29524 -LRB- denotes (
T16850 29524-29533 NNP denotes Cambridge
T16851 29533-29535 , denotes ,
T16852 29535-29548 NNP denotes Massachusetts
T16853 29548-29550 , denotes ,
T16854 29550-29556 NNP denotes United
T16855 29557-29563 NNP denotes States
T16856 29563-29564 -RRB- denotes )
T16857 29564-29565 . denotes .
R1000 T5568 T5555 punct .,identified
R1001 T5512 T5513 det this,interval
R1002 T5570 T5571 amod Pulmonary,hypoplasia
R1003 T5513 T5511 pobj interval,within
R1004 T5514 T5504 punct ", ",tested
R1005 T5515 T5516 quantmod an,39
R1006 T5516 T5518 nummod 39,progeny
R1007 T5571 T5572 nsubj hypoplasia,was
R1008 T5517 T5516 amod additional,39
R1009 T5518 T5504 nsubjpass progeny,tested
R1010 T5573 T5572 acomp apparent,was
R1011 T5519 T5518 compound F2,progeny
R1012 T5520 T5518 prep from,progeny
R1013 T5521 T5522 det an,cross
R1014 T5574 T5572 prep in,was
R1015 T5522 T5520 pobj cross,from
R1016 T5523 T5524 nmod A,J
R1017 T5524 T5522 nmod J,cross
R1018 T5575 T5576 det all,mice
R1019 T5525 T5524 punct /,J
R1020 T5526 T5524 punct ×,J
R1021 T5576 T5574 pobj mice,in
R1022 T5527 T5528 compound C57BL,6J
R1023 T5528 T5524 appos 6J,J
R1024 T5529 T5528 punct /,6J
R1025 T5530 T5504 auxpass were,tested
R1026 T5577 T5576 compound mutant,mice
R1027 T5531 T5504 punct .,tested
R1028 T5578 T5579 dep that,survived
R1029 T5533 T5534 det The,identification
R1030 T5534 T5535 nsubj identification,established
R1031 T5579 T5576 relcl survived,mice
R1032 T5536 T5534 prep of,identification
R1033 T5537 T5538 nummod two,recombinants
R1034 T5538 T5536 pobj recombinants,of
R1035 T5539 T5535 dobj D15Mit85,established
R1036 T5580 T5579 prep to,survived
R1037 T5540 T5535 prep as,established
R1038 T5541 T5542 det the,proximal
R1039 T5542 T5540 pobj proximal,as
R1040 T5581 T5580 pobj birth,to
R1041 T5543 T5535 cc and,established
R1042 T5544 T5535 conj D15Mit5,established
R1043 T5582 T5572 punct .,was
R1044 T5545 T5544 prep as,D15Mit5
R1045 T5546 T5547 det the,distal
R1046 T5547 T5545 pobj distal,as
R1047 T5584 T5585 prep In,were
R1048 T5586 T5587 det a,comparison
R1049 T5587 T5584 pobj comparison,In
R1050 T5655 T5653 prep from,lungs
R1051 T5656 T5657 compound mutant,mice
R1052 T5588 T5587 prep between,comparison
R1053 T5657 T5655 pobj mice,from
R1054 T5658 T5654 aux were,lacking
R1055 T5659 T5660 det an,lobe
R1056 T5589 T5590 amod wild,type
R1057 T5660 T5654 dobj lobe,lacking
R1058 T5661 T5660 amod accessory,lobe
R1059 T5590 T5592 nmod type,mice
R1060 T5662 T5654 prep on,lacking
R1061 T5663 T5664 det the,side
R1062 T5664 T5662 pobj side,on
R1063 T5591 T5590 punct -,type
R1064 T5665 T5664 amod right,side
R1065 T5592 T5588 pobj mice,between
R1066 T5666 T5654 cc and,lacking
R1067 T5667 T5654 conj had,lacking
R1068 T5668 T5667 dobj underdevelopment,had
R1069 T5669 T5668 prep of,underdevelopment
R1070 T5593 T5590 cc and,type
R1071 T5670 T5671 det the,lobe
R1072 T5671 T5669 pobj lobe,of
R1073 T5672 T5671 amod anterior,lobe
R1074 T5594 T5590 conj mutant,type
R1075 T5673 T5671 amod right,lobe
R1076 T5674 T5671 amod middle,lobe
R1077 T5595 T5592 acl found,mice
R1078 T5675 T5676 punct (,Figure
R1079 T5676 T5667 parataxis Figure,had
R1080 T5677 T5676 nummod 1A,Figure
R1081 T5596 T5595 oprd dead,found
R1082 T5678 T5676 punct ),Figure
R1083 T5679 T5654 punct .,lacking
R1084 T5597 T5595 prep on,found
R1085 T5681 T5682 nsubj Diaphragms,were
R1086 T5683 T5681 prep from,Diaphragms
R1087 T5598 T5597 pobj day,on
R1088 T5684 T5685 compound mutant,mice
R1089 T5685 T5683 pobj mice,from
R1090 T5686 T5685 compound lil,mice
R1091 T5599 T5598 nummod one,day
R1092 T5600 T5598 prep of,day
R1093 T5687 T5682 acomp intact,were
R1094 T5688 T5682 punct ", ",were
R1095 T5689 T5682 cc but,were
R1096 T5601 T5600 pobj life,of
R1097 T5690 T5691 nsubj muscularization,was
R1098 T5691 T5682 conj was,were
R1099 T5692 T5691 acomp absent,was
R1100 T5693 T5691 prep in,was
R1101 T5694 T5695 det the,regions
R1102 T5695 T5693 pobj regions,in
R1103 T5602 T5585 punct ", ",were
R1104 T5696 T5695 amod dorsal,regions
R1105 T5697 T5691 punct .,was
R1106 T5603 T5604 compound body,weights
R1107 T5699 T5700 nsubj Myotubules,were
R1108 T5701 T5700 acomp present,were
R1109 T5604 T5605 nsubj weights,were
R1110 T5702 T5700 prep in,were
R1111 T5703 T5704 det a,distribution
R1112 T5704 T5702 pobj distribution,in
R1113 T5705 T5704 amod limited,distribution
R1114 T5605 T5585 ccomp were,were
R1115 T5706 T5705 cc and,limited
R1116 T5707 T5705 conj abnormal,limited
R1117 T5708 T5700 punct .,were
R1118 T5606 T5605 neg not,were
R1119 T5710 T5711 advmod More,specifically
R1120 T5607 T5605 acomp different,were
R1121 T5711 T5712 advmod specifically,radiate
R1122 T5713 T5712 punct ", ",radiate
R1123 T5608 T5585 punct ;,were
R1124 T5714 T5712 nsubj myotubules,radiate
R1125 T5715 T5712 advmod normally,radiate
R1126 T5716 T5712 prep in,radiate
R1127 T5717 T5718 det a,fashion
R1128 T5609 T5585 advmod however,were
R1129 T5718 T5716 pobj fashion,in
R1130 T5719 T5718 amod mediolateral,fashion
R1131 T5610 T5585 punct ", ",were
R1132 T5720 T5721 aux to,meet
R1133 T5721 T5712 advcl meet,radiate
R1134 T5722 T5723 det the,walls
R1135 T5611 T5612 det the,weights
R1136 T5723 T5721 dobj walls,meet
R1137 T5724 T5723 amod lateral,walls
R1138 T5725 T5723 compound body,walls
R1139 T5726 T5712 punct ", ",radiate
R1140 T5612 T5585 nsubj weights,were
R1141 T5727 T5712 prep with,radiate
R1142 T5728 T5729 det a,paucity
R1143 T5613 T5612 compound lung,weights
R1144 T5729 T5727 pobj paucity,with
R1145 T5730 T5729 amod normal,paucity
R1146 T5731 T5729 prep of,paucity
R1147 T5614 T5615 advmod significantly,lower
R1148 T5732 T5733 compound muscle,fibers
R1149 T5733 T5731 pobj fibers,of
R1150 T5734 T5729 prep in,paucity
R1151 T5735 T5736 det the,region
R1152 T5736 T5734 pobj region,in
R1153 T5737 T5736 amod central,region
R1154 T5738 T5736 amod tendonous,region
R1155 T5615 T5585 acomp lower,were
R1156 T5739 T5712 punct .,radiate
R1157 T5616 T5585 prep in,were
R1158 T5741 T5742 prep In,radiated
R1159 T5617 T5618 det the,mice
R1160 T5743 T5744 det the,diaphragm
R1161 T5744 T5741 pobj diaphragm,In
R1162 T5745 T5744 compound mutant,diaphragm
R1163 T5746 T5742 punct ", ",radiated
R1164 T5618 T5616 pobj mice,in
R1165 T5747 T5742 nsubj myotubules,radiated
R1166 T5748 T5742 prep in,radiated
R1167 T5749 T5750 det a,orientation
R1168 T5619 T5618 compound mutant,mice
R1169 T5750 T5748 pobj orientation,in
R1170 T5751 T5752 amod dorsal,ventral
R1171 T5620 T5585 punct .,were
R1172 T5752 T5750 amod ventral,orientation
R1173 T5753 T5752 punct –,ventral
R1174 T5754 T5742 punct ", ",radiated
R1175 T5622 T5623 det The,weight
R1176 T5755 T5742 cc and,radiated
R1177 T5756 T5757 compound muscle,tissue
R1178 T5757 T5758 nsubj tissue,meet
R1179 T5758 T5742 conj meet,radiated
R1180 T5623 T5627 nsubj weight,was
R1181 T5759 T5758 aux did,meet
R1182 T5624 T5623 amod average,weight
R1183 T5625 T5626 compound mutant,lung
R1184 T5626 T5623 compound lung,weight
R1185 T5760 T5758 neg not,meet
R1186 T5761 T5762 det the,surface
R1187 T5628 T5629 quantmod 9.6,2.5
R1188 T5762 T5758 dobj surface,meet
R1189 T5763 T5762 amod entire,surface
R1190 T5764 T5762 prep of,surface
R1191 T5765 T5766 det the,walls
R1192 T5629 T5631 nummod 2.5,mg
R1193 T5766 T5764 pobj walls,of
R1194 T5767 T5766 compound body,walls
R1195 T5768 T5769 punct (,Figure
R1196 T5630 T5629 punct ±,2.5
R1197 T5769 T5758 parataxis Figure,meet
R1198 T5770 T5769 nummod 1B,Figure
R1199 T5771 T5769 punct ),Figure
R1200 T5631 T5627 attr mg,was
R1201 T5772 T5758 punct .,meet
R1202 T5774 T5775 det The,number
R1203 T5632 T5633 mark while,was
R1204 T5775 T5776 nsubj number,was
R1205 T5633 T5627 advcl was,was
R1206 T5777 T5775 prep of,number
R1207 T5778 T5779 compound lil,mice
R1208 T5634 T5635 det the,weight
R1209 T5779 T5777 pobj mice,of
R1210 T5780 T5779 compound mutant,mice
R1211 T5781 T5782 dep that,survived
R1212 T5635 T5633 nsubj weight,was
R1213 T5782 T5779 relcl survived,mice
R1214 T5783 T5782 prep to,survived
R1215 T5784 T5783 pobj birth,to
R1216 T5636 T5635 amod average,weight
R1217 T5785 T5786 amod less,5
R1218 T5786 T5788 nummod 5,%
R1219 T5787 T5786 quantmod than,5
R1220 T5637 T5638 amod wild,type
R1221 T5788 T5776 attr %,was
R1222 T5789 T5788 prep of,%
R1223 T5638 T5640 compound type,lung
R1224 T5790 T5791 amod total,progeny
R1225 T5791 T5789 pobj progeny,of
R1226 T5792 T5776 punct ", ",was
R1227 T5793 T5794 advmod rather,than
R1228 T5639 T5638 punct -,type
R1229 T5794 T5776 prep than,was
R1230 T5795 T5796 det the,%
R1231 T5640 T5635 compound lung,weight
R1232 T5796 T5794 pobj %,than
R1233 T5797 T5796 nummod 25,%
R1234 T5641 T5642 quantmod 26.4,4.6
R1235 T5642 T5644 nummod 4.6,mg
R1236 T5798 T5796 acl expected,%
R1237 T5799 T5798 prep for,expected
R1238 T5643 T5642 punct ±,4.6
R1239 T5800 T5801 det a,mutation
R1240 T5801 T5799 pobj mutation,for
R1241 T5802 T5801 amod recessive,mutation
R1242 T5644 T5633 attr mg,was
R1243 T5803 T5776 punct .,was
R1244 T5645 T5646 punct (,0.001
R1245 T5805 T5806 nsubj We,evaluated
R1246 T5807 T5806 dobj litters,evaluated
R1247 T5646 T5627 parataxis 0.001,was
R1248 T5808 T5806 prep at,evaluated
R1249 T5809 T5810 amod different,points
R1250 T5810 T5808 pobj points,at
R1251 T5647 T5646 nsubj p,0.001
R1252 T5811 T5810 amod embryonic,points
R1253 T5812 T5810 compound time,points
R1254 T5813 T5814 aux to,determine
R1255 T5814 T5806 advcl determine,evaluated
R1256 T5815 T5816 mark whether,was
R1257 T5816 T5814 ccomp was,determine
R1258 T5648 T5646 punct <,0.001
R1259 T5817 T5818 det the,number
R1260 T5818 T5816 nsubj number,was
R1261 T5819 T5818 amod reduced,number
R1262 T5820 T5816 prep due,was
R1263 T5649 T5646 punct ),0.001
R1264 T5821 T5820 pcomp to,due
R1265 T5822 T5823 amod intra-uterine,demise
R1266 T5650 T5627 punct .,was
R1267 T5823 T5820 pobj demise,due
R1268 T5824 T5806 punct .,evaluated
R1269 T5826 T5827 nsubjpass Litters,collected
R1270 T5652 T5653 det All,lungs
R1271 T5828 T5827 auxpass were,collected
R1272 T5829 T5827 prep at,collected
R1273 T5830 T5829 pobj E12.5,at
R1274 T5653 T5654 nsubj lungs,lacking
R1275 T5831 T5830 punct ", ",E12.5
R1276 T5832 T5830 nummod 13.5,E12.5
R1277 T5833 T5830 punct ", ",E12.5
R1278 T5867 T5868 det a,rate
R1279 T5834 T5830 nummod 15.5,E12.5
R1280 T5835 T5830 punct ", ",E12.5
R1281 T5836 T5830 nummod 17.5,E12.5
R1282 T5837 T5830 punct ", ",E12.5
R1283 T5838 T5830 cc and,E12.5
R1284 T5839 T5830 conj 18.5,E12.5
R1285 T5840 T5827 punct ", ",collected
R1286 T5841 T5827 cc and,collected
R1287 T5842 T5843 nsubjpass embryos,genotyped
R1288 T5868 T5866 dobj rate,had
R1289 T5843 T5827 conj genotyped,collected
R1290 T5844 T5843 auxpass were,genotyped
R1291 T5845 T5843 cc and,genotyped
R1292 T5869 T5870 advmod progressively,higher
R1293 T5846 T5843 conj evaluated,genotyped
R1294 T5847 T5846 prep for,evaluated
R1295 T5848 T5847 pobj evidence,for
R1296 T5870 T5868 amod higher,rate
R1297 T5849 T5848 prep of,evidence
R1298 T5850 T5851 amod intra-uterine,demise
R1299 T5871 T5868 prep of,rate
R1300 T5851 T5849 pobj demise,of
R1301 T5852 T5848 punct ", ",evidence
R1302 T5853 T5848 prep including,evidence
R1303 T5872 T5871 pobj demise,of
R1304 T5854 T5855 compound growth,retardation
R1305 T5855 T5853 pobj retardation,including
R1306 T5856 T5855 punct ", ",retardation
R1307 T5857 T5855 conj pallor,retardation
R1308 T5858 T5857 punct ", ",pallor
R1309 T5873 T5866 prep between,had
R1310 T5859 T5857 cc and,pallor
R1311 T5860 T5861 compound tissue,friability
R1312 T5874 T5873 pobj E13.5,between
R1313 T5861 T5857 conj friability,pallor
R1314 T5875 T5874 cc and,E13.5
R1315 T5862 T5843 punct .,genotyped
R1316 T5876 T5874 conj E15.5,E13.5
R1317 T5864 T5865 compound lil,embryos
R1318 T5865 T5866 nsubj embryos,had
R1319 T5877 T5866 punct .,had
R1320 T5879 T5880 prep At,were
R1321 T5881 T5879 pobj E12.5,At
R1322 T5972 T5973 advmod only,embryo
R1323 T5973 T5970 nsubj embryo,was
R1324 T5882 T5880 punct ", ",were
R1325 T5974 T5973 nummod one,embryo
R1326 T5975 T5970 acomp viable,was
R1327 T5976 T5953 punct .,were
R1328 T5883 T5884 quantmod 20,87
R1329 T5978 T5979 amod Diaphragmatic,muscularization
R1330 T5979 T5980 nsubj muscularization,was
R1331 T5884 T5880 nsubj 87,were
R1332 T5981 T5980 acomp abnormal,was
R1333 T5982 T5980 prep in,was
R1334 T5885 T5884 quantmod out,87
R1335 T5983 T5984 det all,mice
R1336 T5886 T5884 quantmod of,87
R1337 T5984 T5982 pobj mice,in
R1338 T5887 T5888 punct (,%
R1339 T5985 T5984 compound mutant,mice
R1340 T5986 T5984 acl examined,mice
R1341 T5987 T5988 punct (,25
R1342 T5988 T5980 parataxis 25,was
R1343 T5888 T5884 parataxis %,87
R1344 T5989 T5988 nsubj n,25
R1345 T5990 T5988 punct >,25
R1346 T5991 T5988 punct ),25
R1347 T5889 T5888 nummod 23,%
R1348 T5992 T5980 punct .,was
R1349 T5890 T5888 punct ),%
R1350 T5994 T5995 amod Pulmonary,hypoplasia
R1351 T5995 T5996 nsubjpass hypoplasia,observed
R1352 T5891 T5880 acomp homozygous,were
R1353 T5997 T5996 auxpass was,observed
R1354 T5998 T5996 prep in,observed
R1355 T5999 T6000 nummod 100,%
R1356 T5892 T5891 prep for,homozygous
R1357 T6000 T5998 pobj %,in
R1358 T6001 T6000 prep of,%
R1359 T6002 T6001 pobj mutants,of
R1360 T6003 T6002 acl evaluated,mutants
R1361 T6004 T6003 prep for,evaluated
R1362 T6005 T6006 det that,phenotype
R1363 T5893 T5894 det the,mutation
R1364 T6006 T6004 pobj phenotype,for
R1365 T6007 T6003 prep between,evaluated
R1366 T6008 T6007 pobj E11.5,between
R1367 T5894 T5892 pobj mutation,for
R1368 T6009 T6008 cc and,E11.5
R1369 T6010 T6008 conj birth,E11.5
R1370 T6011 T6012 punct (,50
R1371 T5895 T5880 punct ", ",were
R1372 T6012 T5996 parataxis 50,observed
R1373 T6013 T6012 nsubj n,50
R1374 T6014 T6012 punct >,50
R1375 T6015 T6012 punct ),50
R1376 T5896 T5880 cc and,were
R1377 T6016 T5996 punct .,observed
R1378 T5897 T5898 nsubj all,appeared
R1379 T6018 T6019 nsubj Examination,showed
R1380 T6020 T6018 prep of,Examination
R1381 T5898 T5880 conj appeared,were
R1382 T5899 T5897 prep of,all
R1383 T6021 T6022 amod cardiac,morphology
R1384 T5900 T5899 pobj these,of
R1385 T6022 T6020 pobj morphology,of
R1386 T6023 T6024 mark that,had
R1387 T6024 T6019 ccomp had,showed
R1388 T5901 T5898 oprd viable,appeared
R1389 T6025 T6024 nsubj hearts,had
R1390 T6026 T6025 prep from,hearts
R1391 T5902 T5880 punct .,were
R1392 T6027 T6028 compound E15.5,mice
R1393 T5904 T5905 prep At,were
R1394 T6028 T6026 pobj mice,from
R1395 T6029 T6030 compound lil,mutant
R1396 T6030 T6028 compound mutant,mice
R1397 T6031 T6032 det a,variety
R1398 T6032 T6024 dobj variety,had
R1399 T5906 T5904 pobj E13.5,At
R1400 T6033 T6032 prep of,variety
R1401 T6034 T6035 amod developmental,defects
R1402 T6035 T6033 pobj defects,of
R1403 T6036 T6032 punct ", ",variety
R1404 T5907 T5905 punct ", ",were
R1405 T6037 T6032 prep including,variety
R1406 T6038 T6039 amod enlarged,cushions
R1407 T5908 T5909 quantmod 13,49
R1408 T5909 T5905 nsubj 49,were
R1409 T6039 T6037 pobj cushions,including
R1410 T6040 T6038 cc and,enlarged
R1411 T6041 T6042 advmod abnormally,developed
R1412 T6042 T6038 conj developed,enlarged
R1413 T6043 T6039 amod endocardial,cushions
R1414 T6044 T6039 punct ", ",cushions
R1415 T5910 T5909 quantmod of,49
R1416 T6045 T6046 det a,ventricle
R1417 T6046 T6039 conj ventricle,cushions
R1418 T6047 T6048 amod double,outlet
R1419 T5911 T5912 punct (,%
R1420 T6048 T6046 nmod outlet,ventricle
R1421 T6049 T6048 punct -,outlet
R1422 T6050 T6046 amod right,ventricle
R1423 T5912 T5909 parataxis %,49
R1424 T6051 T6046 punct ", ",ventricle
R1425 T6052 T6046 cc and,ventricle
R1426 T6053 T6054 det a,canal
R1427 T5913 T5912 nummod 27,%
R1428 T6054 T6046 conj canal,ventricle
R1429 T6055 T6054 amod complete,canal
R1430 T6056 T6054 amod atrioventricular,canal
R1431 T5914 T5912 punct ),%
R1432 T6057 T6019 punct .,showed
R1433 T5915 T5905 acomp mutant,were
R1434 T6059 T6060 det The,myocardium
R1435 T6060 T6061 nsubj myocardium,was
R1436 T5916 T5905 cc and,were
R1437 T6062 T6061 advmod also,was
R1438 T6063 T6064 advmod poorly,developed
R1439 T5917 T5918 nsubj two,died
R1440 T6064 T6061 acomp developed,was
R1441 T6065 T6061 punct ", ",was
R1442 T6066 T6061 prep with,was
R1443 T6067 T6066 pobj thinning,with
R1444 T5918 T5905 conj died,were
R1445 T6068 T6067 prep of,thinning
R1446 T6069 T6070 det the,layer
R1447 T5919 T5918 aux had,died
R1448 T6070 T6068 pobj layer,of
R1449 T6071 T6070 amod outer,layer
R1450 T6072 T6070 amod compact,layer
R1451 T5920 T5905 punct .,were
R1452 T6073 T6067 cc and,thinning
R1453 T6074 T6075 amod decreased,vascularity
R1454 T5922 T5923 prep At,were
R1455 T6075 T6067 conj vascularity,thinning
R1456 T6076 T6061 punct .,was
R1457 T5924 T5922 pobj E15.5,At
R1458 T5925 T5923 punct ", ",were
R1459 T6078 T6079 det The,cohort
R1460 T5926 T5927 quantmod 22,91
R1461 T6079 T6080 nsubj cohort,had
R1462 T6081 T6079 prep of,cohort
R1463 T6082 T6083 compound mutant,mice
R1464 T6083 T6081 pobj mice,of
R1465 T6084 T6085 dep that,survived
R1466 T6085 T6083 relcl survived,mice
R1467 T5927 T5923 nsubj 91,were
R1468 T6086 T6085 prep to,survived
R1469 T6087 T6086 pobj birth,to
R1470 T6088 T6080 advmod also,had
R1471 T5928 T5927 quantmod out,91
R1472 T6089 T6090 amod cardiac,malformations
R1473 T6090 T6080 dobj malformations,had
R1474 T6091 T6090 prep including,malformations
R1475 T5929 T5927 quantmod of,91
R1476 T6092 T6093 amod atrioventricular,canal
R1477 T6093 T6095 nmod canal,type
R1478 T6094 T6093 punct -,canal
R1479 T5930 T5931 punct (,%
R1480 T6095 T6097 nmod type,defects
R1481 T6096 T6095 punct -,type
R1482 T5931 T5927 parataxis %,91
R1483 T6097 T6091 pobj defects,including
R1484 T6098 T6097 amod ventricular,defects
R1485 T6099 T6097 amod septal,defects
R1486 T5932 T5931 nummod 24,%
R1487 T6100 T6097 punct ", ",defects
R1488 T6101 T6102 nmod ostium,defects
R1489 T6102 T6097 conj defects,defects
R1490 T5933 T5931 punct ),%
R1491 T6103 T6102 nmod primum,defects
R1492 T6104 T6102 amod atrial,defects
R1493 T5934 T5923 acomp mutant,were
R1494 T6105 T6102 amod septal,defects
R1495 T6106 T6102 punct ", ",defects
R1496 T6107 T6102 cc and,defects
R1497 T5935 T5923 cc and,were
R1498 T6108 T6109 amod enlarged,atria
R1499 T6109 T6102 conj atria,defects
R1500 T6110 T6111 punct (,shown
R1501 T5936 T5937 det the,majority
R1502 T6111 T6080 parataxis shown,had
R1503 T6112 T6111 nsubj data,shown
R1504 T5937 T5938 nsubj majority,died
R1505 T6113 T6111 neg not,shown
R1506 T6114 T6111 punct ),shown
R1507 T6115 T6080 punct .,had
R1508 T5938 T5923 conj died,were
R1509 T6117 T6118 det All,mutants
R1510 T6118 T6119 nsubj mutants,had
R1511 T5939 T5937 prep of,majority
R1512 T6120 T6121 advmod specifically,evaluated
R1513 T6121 T6118 acl evaluated,mutants
R1514 T6122 T6121 prep for,evaluated
R1515 T6123 T6124 det a,phenotype
R1516 T6124 T6122 pobj phenotype,for
R1517 T5940 T5941 compound mutant,embryos
R1518 T6125 T6124 amod cardiac,phenotype
R1519 T5941 T5939 pobj embryos,of
R1520 T6126 T6127 punct (,10
R1521 T6127 T6118 parataxis 10,mutants
R1522 T6128 T6127 nsubj n,10
R1523 T5942 T5943 punct (,22
R1524 T6129 T6127 punct =,10
R1525 T6130 T6127 punct ),10
R1526 T5943 T5937 parataxis 22,majority
R1527 T6131 T6132 amod abnormal,development
R1528 T6132 T6119 dobj development,had
R1529 T6133 T6132 amod cardiac,development
R1530 T6134 T6119 punct .,had
R1531 T5944 T5943 quantmod 17,22
R1532 T6136 T6137 nsubj Examination,revealed
R1533 T5945 T5943 quantmod out,22
R1534 T6138 T6136 prep of,Examination
R1535 T5946 T5943 quantmod of,22
R1536 T6139 T6140 det the,region
R1537 T6140 T6138 pobj region,of
R1538 T6141 T6142 nummod 3,Mb
R1539 T6142 T6140 compound Mb,region
R1540 T5947 T5943 quantmod the,22
R1541 T6143 T6142 punct -,Mb
R1542 T6144 T6140 prep between,region
R1543 T5948 T5943 punct ),22
R1544 T6145 T6144 pobj D15Mit85,between
R1545 T6146 T6145 cc and,D15Mit85
R1546 T6147 T6145 conj D15Mit5,D15Mit85
R1547 T5949 T5938 aux had,died
R1548 T6148 T6140 prep in,region
R1549 T6149 T6150 compound DNA,sequence
R1550 T6150 T6151 compound sequence,databases
R1551 T5950 T5938 punct .,died
R1552 T6151 T6148 pobj databases,in
R1553 T6152 T6153 nummod three,genes
R1554 T6153 T6137 dobj genes,revealed
R1555 T5952 T5953 prep By,were
R1556 T6154 T6153 amod predicted,genes
R1557 T6155 T6153 cc and,genes
R1558 T6156 T6157 nummod four,genes
R1559 T6157 T6153 conj genes,genes
R1560 T6158 T6157 amod known,genes
R1561 T5954 T5952 pobj E18.5,By
R1562 T6159 T6157 punct ", ",genes
R1563 T6160 T6157 prep including,genes
R1564 T6161 T6160 pobj Fog2,including
R1565 T6162 T6137 punct .,revealed
R1566 T6164 T6165 amod Targeted,mutations
R1567 T5955 T5953 punct ", ",were
R1568 T6165 T6166 nsubj mutations,have
R1569 T6167 T6165 prep of,mutations
R1570 T5956 T5957 quantmod nine,29
R1571 T6168 T6167 pobj Fog2,of
R1572 T6169 T6170 amod cardiac,defects
R1573 T6170 T6166 dobj defects,have
R1574 T6171 T6172 advmod strikingly,similar
R1575 T5957 T5959 nummod 29,embryos
R1576 T6172 T6170 amod similar,defects
R1577 T6173 T6172 prep to,similar
R1578 T6174 T6173 pobj those,to
R1579 T5958 T5957 quantmod of,29
R1580 T6175 T6176 nsubj we,identified
R1581 T6176 T6174 advcl identified,those
R1582 T5959 T5953 nsubj embryos,were
R1583 T6177 T6176 prep in,identified
R1584 T5960 T5961 punct (,%
R1585 T6178 T6179 compound lil,mice
R1586 T6179 T6177 pobj mice,in
R1587 T6180 T6179 compound mutant,mice
R1588 T6181 T6170 punct ", ",defects
R1589 T5961 T5959 parataxis %,embryos
R1590 T6182 T6170 prep including,defects
R1591 T6183 T6184 amod atrioventricular,defects
R1592 T5962 T5961 nummod 31,%
R1593 T5963 T5961 punct ),%
R1594 T5964 T5953 acomp homozygous,were
R1595 T6184 T6182 pobj defects,including
R1596 T5965 T5964 prep for,homozygous
R1597 T6185 T6184 compound canal,defects
R1598 T6186 T6184 punct ", ",defects
R1599 T6187 T6188 amod thinned,myocardium
R1600 T5966 T5967 det the,mutation
R1601 T6188 T6184 conj myocardium,defects
R1602 T6189 T6188 punct ", ",myocardium
R1603 T6190 T6188 cc and,myocardium
R1604 T5967 T5965 pobj mutation,for
R1605 T6191 T6192 amod absent,vasculature
R1606 T6192 T6188 conj vasculature,myocardium
R1607 T6193 T6192 amod coronary,vasculature
R1608 T5968 T5953 punct ", ",were
R1609 T5969 T5970 prep of,was
R1610 T6194 T6195 punct [,16
R1611 T5970 T5953 ccomp was,were
R1612 T6195 T6166 parataxis 16,have
R1613 T5971 T5969 pobj which,of
R1614 T6196 T6195 punct ],16
R1615 T6197 T6166 punct .,have
R1616 T6290 T6291 det a,codon
R1617 T6199 T6200 compound RT,PCR
R1618 T6200 T6202 compound PCR,amplification
R1619 T6201 T6200 punct -,PCR
R1620 T6202 T6203 nsubj amplification,revealed
R1621 T6204 T6202 prep of,amplification
R1622 T6205 T6206 det the,portion
R1623 T6206 T6204 pobj portion,of
R1624 T6207 T6206 amod proximal,portion
R1625 T6208 T6206 prep of,portion
R1626 T6209 T6208 pobj Fog2,of
R1627 T6210 T6211 amod longer,transcripts
R1628 T6211 T6203 dobj transcripts,revealed
R1629 T6291 T6289 dobj codon,introduces
R1630 T6212 T6211 prep in,transcripts
R1631 T6213 T6214 det the,mutant
R1632 T6292 T6291 compound stop,codon
R1633 T6214 T6212 pobj mutant,in
R1634 T6215 T6214 compound lil,mutant
R1635 T6216 T6211 prep than,transcripts
R1636 T6293 T6294 dep that,generates
R1637 T6217 T6216 prep in,than
R1638 T6218 T6219 det the,type
R1639 T6219 T6217 pobj type,in
R1640 T6294 T6291 relcl generates,codon
R1641 T6220 T6219 amod wild,type
R1642 T6221 T6219 punct -,type
R1643 T6295 T6296 det a,product
R1644 T6222 T6211 appos embryos,transcripts
R1645 T6223 T6224 punct (,Figure
R1646 T6224 T6203 parataxis Figure,revealed
R1647 T6296 T6294 dobj product,generates
R1648 T6225 T6224 nummod 2A,Figure
R1649 T6226 T6224 punct ),Figure
R1650 T6227 T6203 punct .,revealed
R1651 T6229 T6230 nsubj Sequencing,revealed
R1652 T6297 T6298 advmod severely,truncated
R1653 T6231 T6229 prep of,Sequencing
R1654 T6232 T6233 det the,transcript
R1655 T6298 T6296 amod truncated,product
R1656 T6233 T6231 pobj transcript,of
R1657 T6234 T6233 compound mutant,transcript
R1658 T6235 T6236 det a,mutation
R1659 T6299 T6296 compound protein,product
R1660 T6236 T6230 dobj mutation,revealed
R1661 T6237 T6236 compound point,mutation
R1662 T6238 T6236 punct (,mutation
R1663 T6300 T6301 punct (,Figure
R1664 T6239 T6236 prep from,mutation
R1665 T6240 T6239 pobj thymine,from
R1666 T6301 T6289 parataxis Figure,introduces
R1667 T6241 T6239 prep to,from
R1668 T6242 T6241 pobj cytosine,to
R1669 T6243 T6236 punct ),mutation
R1670 T6244 T6245 nummod 2,bp
R1671 T6245 T6246 npadvmod bp,after
R1672 T6246 T6230 prep after,revealed
R1673 T6302 T6301 nummod 2B,Figure
R1674 T6247 T6246 pobj position,after
R1675 T6248 T6247 nummod 301,position
R1676 T6303 T6301 punct ),Figure
R1677 T6249 T6247 punct ", ",position
R1678 T6250 T6251 dep which,is
R1679 T6251 T6247 relcl is,position
R1680 T6304 T6268 punct .,causes
R1681 T6252 T6251 prep in,is
R1682 T6253 T6254 det the,site
R1683 T6254 T6252 pobj site,in
R1684 T6306 T6307 amod Heterozygous,mice
R1685 T6255 T6254 compound splice,site
R1686 T6256 T6254 compound donor,site
R1687 T6257 T6254 prep at,site
R1688 T6258 T6259 det the,end
R1689 T6259 T6257 pobj end,at
R1690 T6307 T6310 nsubjpass mice,crossed
R1691 T6260 T6259 prep of,end
R1692 T6261 T6262 det the,exon
R1693 T6262 T6260 pobj exon,of
R1694 T6308 T6309 compound lil,mutant
R1695 T6263 T6262 amod third,exon
R1696 T6264 T6230 punct .,revealed
R1697 T6309 T6307 compound mutant,mice
R1698 T6266 T6267 det This,mutation
R1699 T6267 T6268 nsubj mutation,causes
R1700 T6311 T6310 auxpass were,crossed
R1701 T6269 T6270 det a,defect
R1702 T6270 T6268 dobj defect,causes
R1703 T6271 T6270 compound splicing,defect
R1704 T6272 T6273 dep that,results
R1705 T6312 T6310 prep with,crossed
R1706 T6273 T6270 relcl results,defect
R1707 T6274 T6273 prep in,results
R1708 T6313 T6314 det a,mutant
R1709 T6275 T6276 det the,insertion
R1710 T6276 T6274 pobj insertion,in
R1711 T6277 T6276 prep of,insertion
R1712 T6314 T6312 pobj mutant,with
R1713 T6278 T6279 nummod 85,bp
R1714 T6279 T6277 pobj bp,of
R1715 T6315 T6314 nmod Fog2,mutant
R1716 T6280 T6279 prep of,bp
R1717 T6281 T6282 amod intronic,sequence
R1718 T6316 T6315 punct +,Fog2
R1719 T6282 T6280 pobj sequence,of
R1720 T6283 T6276 prep into,insertion
R1721 T6284 T6285 det the,transcript
R1722 T6285 T6283 pobj transcript,into
R1723 T6286 T6285 compound mutant,transcript
R1724 T6317 T6315 punct /,Fog2
R1725 T6287 T6268 punct ", ",causes
R1726 T6288 T6268 cc and,causes
R1727 T6289 T6268 conj introduces,causes
R1728 T6318 T6315 punct −,Fog2
R1729 T6319 T6320 punct (,allele
R1730 T6320 T6314 parataxis allele,mutant
R1731 T6321 T6320 amod null,allele
R1732 T6322 T6320 punct ),allele
R1733 T6323 T6314 acl generated,mutant
R1734 T6324 T6323 prep by,generated
R1735 T6325 T6326 compound gene,targeting
R1736 T6326 T6324 pobj targeting,by
R1737 T6327 T6328 punct [,16
R1738 T6328 T6310 parataxis 16,crossed
R1739 T6329 T6328 punct ],16
R1740 T6330 T6310 punct .,crossed
R1741 T6332 T6333 advmod Doubly,heterozygous
R1742 T6333 T6334 amod heterozygous,mice
R1743 T6334 T6335 nsubj mice,had
R1744 T6335 T6336 ccomp had,proves
R1745 T6337 T6338 det an,phenotype
R1746 T6338 T6335 dobj phenotype,had
R1747 T6339 T6338 amod embryonic,phenotype
R1748 T6340 T6338 amod lethal,phenotype
R1749 T6341 T6336 punct ;,proves
R1750 T6342 T6343 det this,failure
R1751 T6343 T6336 nsubj failure,proves
R1752 T6344 T6345 aux to,complement
R1753 T6345 T6343 acl complement,failure
R1754 T6346 T6347 mark that,is
R1755 T6347 T6336 ccomp is,proves
R1756 T6348 T6347 nsubj lil,is
R1757 T6349 T6350 det a,mutation
R1758 T6350 T6347 attr mutation,is
R1759 T6351 T6350 prep in,mutation
R1760 T6352 T6351 pobj Fog2,in
R1761 T6353 T6336 punct .,proves
R1762 T6355 T6356 det The,phenotype
R1763 T6356 T6358 nsubj phenotype,is
R1764 T6357 T6356 amod variable,phenotype
R1765 T6359 T6356 prep of,phenotype
R1766 T6360 T6361 compound lil,mice
R1767 T6361 T6359 pobj mice,of
R1768 T6362 T6356 punct (,phenotype
R1769 T6363 T6356 amod relative,phenotype
R1770 T6364 T6363 prep to,relative
R1771 T6365 T6364 pobj that,to
R1772 T6366 T6365 acl found,that
R1773 T6367 T6366 prep for,found
R1774 T6368 T6369 det the,mutant
R1775 T6369 T6367 pobj mutant,for
R1776 T6370 T6369 nmod Fog2,mutant
R1777 T6371 T6370 amod null,Fog2
R1778 T6372 T6358 punct ),is
R1779 T6373 T6358 advmod likely,is
R1780 T6374 T6358 prep due,is
R1781 T6375 T6374 pcomp to,due
R1782 T6376 T6377 det the,generation
R1783 T6377 T6374 pobj generation,due
R1784 T6378 T6377 prep of,generation
R1785 T6379 T6380 det a,level
R1786 T6380 T6378 pobj level,of
R1787 T6381 T6380 amod low,level
R1788 T6382 T6380 prep of,level
R1789 T6383 T6384 amod normal,transcript
R1790 T6384 T6382 pobj transcript,of
R1791 T6385 T6377 prep despite,generation
R1792 T6386 T6387 det the,presence
R1793 T6387 T6385 pobj presence,despite
R1794 T6388 T6387 prep of,presence
R1795 T6389 T6390 det the,mutation
R1796 T6390 T6388 pobj mutation,of
R1797 T6391 T6392 compound splice,site
R1798 T6392 T6390 compound site,mutation
R1799 T6393 T6358 punct .,is
R1805 T8121 T8122 nmod Lung,Development
R1806 T8123 T8121 cc and,Lung
R1807 T8124 T8121 conj Diaphragm,Lung
R1808 T8125 T8122 prep in,Development
R1809 T8126 T8127 det the,Mouse
R1810 T8127 T8125 pobj Mouse,in
R1811 T8128 T8129 compound Fog2,Mutant
R1812 T8129 T8127 compound Mutant,Mouse
R1813 T8131 T8132 nsubjpass Experiments,conducted
R1814 T8133 T8132 auxpass were,conducted
R1815 T8134 T8135 aux to,evaluate
R1816 T8135 T8132 advcl evaluate,conducted
R1817 T8136 T8137 det the,role
R1818 T8137 T8135 dobj role,evaluate
R1819 T8138 T8137 prep of,role
R1820 T8139 T8138 pobj Fog2,of
R1821 T8140 T8137 prep in,role
R1822 T8141 T8142 amod pulmonary,development
R1823 T8142 T8140 pobj development,in
R1824 T8143 T8141 cc and,pulmonary
R1825 T8144 T8141 conj diaphragmatic,pulmonary
R1826 T8145 T8132 punct .,conducted
R1827 T8147 T8148 det The,phenotype
R1828 T8148 T8150 nsubjpass phenotype,characterized
R1829 T8149 T8148 amod pulmonary,phenotype
R1830 T8151 T8150 auxpass is,characterized
R1831 T8152 T8150 agent by,characterized
R1832 T8153 T8154 amod diffuse,hypoplasia
R1833 T8154 T8152 pobj hypoplasia,by
R1834 T8155 T8154 cc and,hypoplasia
R1835 T8156 T8157 amod specific,loss
R1836 T8157 T8154 conj loss,hypoplasia
R1837 T8158 T8157 prep of,loss
R1838 T8159 T8160 det the,lobe
R1839 T8160 T8158 pobj lobe,of
R1840 T8161 T8160 amod accessory,lobe
R1841 T8162 T8160 cc and,lobe
R1842 T8163 T8164 det a,portion
R1843 T8164 T8160 conj portion,lobe
R1844 T8165 T8164 prep of,portion
R1845 T8166 T8167 det the,lobe
R1846 T8167 T8165 pobj lobe,of
R1847 T8168 T8167 amod right,lobe
R1848 T8169 T8167 amod middle,lobe
R1849 T8170 T8150 punct .,characterized
R1850 T8172 T8173 nsubj It,is
R1851 T8174 T8175 advmod well,established
R1852 T8175 T8173 acomp established,is
R1853 T8176 T8177 mark that,result
R1854 T8177 T8173 ccomp result,is
R1855 T8178 T8177 nsubj abnormalities,result
R1856 T8179 T8178 prep in,abnormalities
R1857 T8180 T8181 preconj either,development
R1858 T8181 T8179 pobj development,in
R1859 T8182 T8181 amod diaphragmatic,development
R1860 T8183 T8181 cc or,development
R1861 T8184 T8185 amod fetal,breathing
R1862 T8185 T8181 conj breathing,development
R1863 T8186 T8177 aux can,result
R1864 T8187 T8177 prep in,result
R1865 T8188 T8189 det a,hypoplasia
R1866 T8189 T8187 pobj hypoplasia,in
R1867 T8190 T8189 amod secondary,hypoplasia
R1868 T8191 T8189 amod pulmonary,hypoplasia
R1869 T8192 T8177 punct ", ",result
R1870 T8193 T8194 mark although,documented
R1871 T8194 T8177 advcl documented,result
R1872 T8195 T8194 nsubjpass loss,documented
R1873 T8196 T8195 prep of,loss
R1874 T8197 T8198 amod normal,structure
R1875 T8198 T8196 pobj structure,of
R1876 T8199 T8194 aux has,documented
R1877 T8200 T8194 neg never,documented
R1878 T8201 T8194 auxpass been,documented
R1879 T8202 T8194 prep in,documented
R1880 T8203 T8204 det this,context
R1881 T8204 T8202 pobj context,in
R1882 T8205 T8206 punct [,18
R1883 T8206 T8173 parataxis 18,is
R1884 T8207 T8206 nummod 17,18
R1885 T8208 T8206 punct ",",18
R1886 T8209 T8206 punct ],18
R1887 T8210 T8173 punct .,is
R1888 T8212 T8213 nsubjpass Fog2,expressed
R1889 T8214 T8213 auxpass is,expressed
R1890 T8215 T8213 advmod diffusely,expressed
R1891 T8216 T8213 prep in,expressed
R1892 T8217 T8218 det the,mesenchyme
R1893 T8218 T8216 pobj mesenchyme,in
R1894 T8219 T8218 amod pulmonary,mesenchyme
R1895 T8220 T8213 prep during,expressed
R1896 T8221 T8222 det the,period
R1897 T8222 T8220 pobj period,during
R1898 T8223 T8222 prep of,period
R1899 T8224 T8225 compound branching,morphogenesis
R1900 T8225 T8223 pobj morphogenesis,of
R1901 T8226 T8213 punct ", ",expressed
R1902 T8227 T8228 mark while,restricted
R1903 T8228 T8213 advcl restricted,expressed
R1904 T8229 T8230 amod later,expression
R1905 T8230 T8228 nsubjpass expression,restricted
R1906 T8231 T8228 auxpass is,restricted
R1907 T8232 T8228 prep to,restricted
R1908 T8233 T8234 det the,muscles
R1909 T8234 T8232 pobj muscles,to
R1910 T8235 T8234 amod smooth,muscles
R1911 T8236 T8234 prep of,muscles
R1912 T8237 T8236 pobj airways,of
R1913 T8238 T8237 cc and,airways
R1914 T8239 T8240 amod pulmonary,vessels
R1915 T8240 T8237 conj vessels,airways
R1916 T8241 T8242 punct (,Figure
R1917 T8242 T8213 parataxis Figure,expressed
R1918 T8243 T8242 nummod 3,Figure
R1919 T8244 T8242 punct ),Figure
R1920 T8245 T8213 punct .,expressed
R1921 T8247 T8248 nsubj This,suggests
R1922 T8249 T8247 punct ", ",This
R1923 T8250 T8247 cc and,This
R1924 T8251 T8252 det the,observation
R1925 T8252 T8247 conj observation,This
R1926 T8253 T8254 mark that,appeared
R1927 T8254 T8252 acl appeared,observation
R1928 T8255 T8254 nsubj lungs,appeared
R1929 T8256 T8254 oprd small,appeared
R1930 T8257 T8254 prep on,appeared
R1931 T8258 T8259 amod transverse,sections
R1932 T8259 T8257 pobj sections,on
R1933 T8260 T8259 acl evaluated,sections
R1934 T8261 T8262 amod prior,to
R1935 T8262 T8260 prep to,evaluated
R1936 T8263 T8264 amod diaphragmatic,muscularization
R1937 T8264 T8262 pobj muscularization,to
R1938 T8265 T8264 cc or,muscularization
R1939 T8266 T8264 conj function,muscularization
R1940 T8267 T8248 punct ", ",suggests
R1941 T8268 T8269 mark that,occurs
R1942 T8269 T8248 ccomp occurs,suggests
R1943 T8270 T8271 det the,hypoplasia
R1944 T8271 T8269 nsubj hypoplasia,occurs
R1945 T8272 T8271 amod pulmonary,hypoplasia
R1946 T8273 T8269 advmod independently,occurs
R1947 T8274 T8273 prep of,independently
R1948 T8275 T8276 det the,defect
R1949 T8276 T8274 pobj defect,of
R1950 T8277 T8276 amod diaphragmatic,defect
R1951 T8278 T8248 punct .,suggests
R1952 T8280 T8281 aux To,test
R1953 T8281 T8282 advcl test,dissected
R1954 T8283 T8284 det this,hypothesis
R1955 T8284 T8281 dobj hypothesis,test
R1956 T8285 T8282 punct ", ",dissected
R1957 T8286 T8282 nsubjpass lungs,dissected
R1958 T8287 T8282 auxpass were,dissected
R1959 T8288 T8282 prep from,dissected
R1960 T8289 T8290 nmod Fog2,mice
R1961 T8290 T8288 pobj mice,from
R1962 T8291 T8289 punct −,Fog2
R1963 T8292 T8289 punct /,Fog2
R1964 T8293 T8289 punct −,Fog2
R1965 T8294 T8290 cc and,mice
R1966 T8295 T8296 compound littermate,controls
R1967 T8296 T8290 conj controls,mice
R1968 T8297 T8282 prep before,dissected
R1969 T8298 T8299 det the,onset
R1970 T8299 T8297 pobj onset,before
R1971 T8300 T8299 prep of,onset
R1972 T8301 T8302 amod fetal,motion
R1973 T8302 T8300 pobj motion,of
R1974 T8303 T8302 amod diaphragmatic,motion
R1975 T8304 T8282 punct .,dissected
R1976 T8306 T8307 nmod Fog2,mice
R1977 T8307 T8311 nsubjpass mice,used
R1978 T8308 T8306 punct −,Fog2
R1979 T8309 T8306 punct /,Fog2
R1980 T8310 T8306 punct −,Fog2
R1981 T8312 T8311 auxpass were,used
R1982 T8313 T8311 prep for,used
R1983 T8314 T8315 det this,experiment
R1984 T8315 T8313 pobj experiment,for
R1985 T8316 T8311 punct ", ",used
R1986 T8317 T8318 mark as,wanted
R1987 T8318 T8311 advcl wanted,used
R1988 T8319 T8318 nsubj we,wanted
R1989 T8320 T8321 aux to,avoid
R1990 T8321 T8318 xcomp avoid,wanted
R1991 T8322 T8323 amod potential,variance
R1992 T8323 T8321 dobj variance,avoid
R1993 T8324 T8323 amod phenotypic,variance
R1994 T8325 T8323 prep from,variance
R1995 T8326 T8327 det the,mutation
R1996 T8327 T8325 pobj mutation,from
R1997 T8328 T8327 nmod lil,mutation
R1998 T8329 T8327 amod hypomorphic,mutation
R1999 T8330 T8311 punct .,used
R2000 T8332 T8333 nsubj Lungs,were
R2001 T8334 T8332 acl dissected,Lungs
R2002 T8335 T8334 prep at,dissected
R2003 T8336 T8335 pobj E12,at
R2004 T8337 T8332 prep from,Lungs
R2005 T8338 T8339 nmod Fog2,embryos
R2006 T8339 T8337 pobj embryos,from
R2007 T8340 T8338 punct −,Fog2
R2008 T8341 T8338 punct /,Fog2
R2009 T8342 T8338 punct −,Fog2
R2010 T8343 T8333 acomp smaller,were
R2011 T8344 T8343 prep in,smaller
R2012 T8345 T8344 pobj size,in
R2013 T8346 T8333 cc and,were
R2014 T8347 T8333 conj lacked,were
R2015 T8348 T8349 det the,development
R2016 T8349 T8347 dobj development,lacked
R2017 T8350 T8349 prep of,development
R2018 T8351 T8352 det an,lobe
R2019 T8352 T8350 pobj lobe,of
R2020 T8353 T8352 amod accessory,lobe
R2021 T8354 T8333 punct .,were
R2022 T8356 T8357 prep In,was
R2023 T8358 T8359 nummod 11,explants
R2024 T8359 T8356 pobj explants,In
R2025 T8360 T8359 amod viable,explants
R2026 T8361 T8359 nmod Fog2,explants
R2027 T8362 T8361 punct −,Fog2
R2028 T8363 T8361 punct /,Fog2
R2029 T8364 T8361 punct −,Fog2
R2030 T8365 T8366 compound lung,culture
R2031 T8366 T8359 compound culture,explants
R2032 T8367 T8357 punct ", ",was
R2033 T8368 T8357 expl there,was
R2034 T8369 T8357 neg never,was
R2035 T8370 T8357 attr development,was
R2036 T8371 T8370 prep of,development
R2037 T8372 T8373 det an,lobe
R2038 T8373 T8371 pobj lobe,of
R2039 T8374 T8373 amod accessory,lobe
R2040 T8375 T8357 punct ", ",was
R2041 T8376 T8357 cc and,was
R2042 T8377 T8378 det the,weights
R2043 T8378 T8379 nsubj weights,were
R2044 T8379 T8357 conj were,was
R2045 T8380 T8378 prep of,weights
R2046 T8381 T8382 compound mutant,lungs
R2047 T8382 T8380 pobj lungs,of
R2048 T8383 T8382 acl cultured,lungs
R2049 T8384 T8383 prep for,cultured
R2050 T8385 T8386 nummod 5,d
R2051 T8386 T8384 pobj d,for
R2052 T8387 T8388 advmod significantly,lower
R2053 T8388 T8379 acomp lower,were
R2054 T8389 T8388 prep than,lower
R2055 T8390 T8389 pobj those,than
R2056 T8391 T8390 prep of,those
R2057 T8392 T8393 compound littermate,controls
R2058 T8393 T8391 pobj controls,of
R2059 T8394 T8395 punct (,Figure
R2060 T8395 T8379 parataxis Figure,were
R2061 T8396 T8395 nummod 4,Figure
R2062 T8397 T8395 punct ),Figure
R2063 T8398 T8357 punct .,was
R2064 T8400 T8401 det These,data
R2065 T8401 T8402 nsubj data,demonstrate
R2066 T8403 T8404 mark that,is
R2067 T8404 T8402 ccomp is,demonstrate
R2068 T8405 T8406 det the,hypoplasia
R2069 T8406 T8404 nsubj hypoplasia,is
R2070 T8407 T8406 amod pulmonary,hypoplasia
R2071 T8408 T8406 prep in,hypoplasia
R2072 T8409 T8410 compound Fog2,mice
R2073 T8410 T8408 pobj mice,in
R2074 T8411 T8410 compound mutant,mice
R2075 T8412 T8413 det a,defect
R2076 T8413 T8404 attr defect,is
R2077 T8414 T8413 amod primary,defect
R2078 T8415 T8402 punct .,demonstrate
R2079 T8417 T8418 nsubj Branching,appeared
R2080 T8419 T8417 prep in,Branching
R2081 T8420 T8421 det the,lobes
R2082 T8421 T8419 pobj lobes,in
R2083 T8422 T8421 amod unaffected,lobes
R2084 T8423 T8424 aux to,delayed
R2085 T8424 T8418 xcomp delayed,appeared
R2086 T8425 T8424 auxpass be,delayed
R2087 T8426 T8424 prep by,delayed
R2088 T8427 T8428 quantmod 6,12
R2089 T8428 T8430 nummod 12,h
R2090 T8429 T8428 punct –,12
R2091 T8430 T8426 pobj h,by
R2092 T8431 T8418 punct ", ",appeared
R2093 T8432 T8418 cc but,appeared
R2094 T8433 T8434 det all,mutants
R2095 T8434 T8435 nsubj mutants,developed
R2096 T8435 T8418 conj developed,appeared
R2097 T8436 T8437 det an,pattern
R2098 T8437 T8435 dobj pattern,developed
R2099 T8438 T8437 amod intricate,pattern
R2100 T8439 T8437 compound branching,pattern
R2101 T8440 T8435 prep in,developed
R2102 T8441 T8442 det the,lobes
R2103 T8442 T8440 pobj lobes,in
R2104 T8443 T8442 amod unaffected,lobes
R2105 T8444 T8435 prep after,developed
R2106 T8445 T8444 pobj culture,after
R2107 T8446 T8445 prep for,culture
R2108 T8447 T8448 nummod 5,d
R2109 T8448 T8446 pobj d,for
R2110 T8449 T8435 punct .,developed
R2111 T8451 T8452 mark Because,is
R2112 T8452 T8456 advcl is,evaluated
R2113 T8453 T8454 det the,phenotype
R2114 T8454 T8452 nsubj phenotype,is
R2115 T8455 T8454 compound Fog2,phenotype
R2116 T8457 T8452 acomp striking,is
R2117 T8458 T8457 prep for,striking
R2118 T8459 T8460 amod specific,loss
R2119 T8460 T8458 pobj loss,for
R2120 T8461 T8460 compound lobar,loss
R2121 T8462 T8456 punct ", ",evaluated
R2122 T8463 T8464 det the,pattern
R2123 T8464 T8456 nsubjpass pattern,evaluated
R2124 T8465 T8464 amod spatial,pattern
R2125 T8466 T8464 prep of,pattern
R2126 T8467 T8468 compound Fog2,expression
R2127 T8468 T8466 pobj expression,of
R2128 T8469 T8456 auxpass was,evaluated
R2129 T8470 T8456 prep in,evaluated
R2130 T8471 T8472 amod normal,embryos
R2131 T8472 T8470 pobj embryos,in
R2132 T8473 T8456 prep during,evaluated
R2133 T8474 T8475 det the,period
R2134 T8475 T8473 pobj period,during
R2135 T8476 T8475 prep of,period
R2136 T8477 T8478 amod early,establishment
R2137 T8478 T8476 pobj establishment,of
R2138 T8479 T8478 compound lobar,establishment
R2139 T8480 T8481 aux to,determine
R2140 T8481 T8456 advcl determine,evaluated
R2141 T8482 T8483 mark whether,is
R2142 T8483 T8481 ccomp is,determine
R2143 T8484 T8485 compound Fog2,expression
R2144 T8485 T8483 nsubj expression,is
R2145 T8486 T8487 advmod specifically,different
R2146 T8487 T8483 acomp different,is
R2147 T8488 T8483 prep at,is
R2148 T8489 T8490 det these,buds
R2149 T8490 T8488 pobj buds,at
R2150 T8491 T8490 compound lobar,buds
R2151 T8492 T8456 punct .,evaluated
R2152 T8494 T8495 nsubjpass Expression,evaluated
R2153 T8496 T8495 auxpass was,evaluated
R2154 T8497 T8495 prep in,evaluated
R2155 T8498 T8497 pobj mice,in
R2156 T8499 T8498 acl carrying,mice
R2157 T8500 T8501 det a,gene
R2158 T8501 T8499 dobj gene,carrying
R2159 T8502 T8501 compound lacZ,gene
R2160 T8503 T8501 acl incorporated,gene
R2161 T8504 T8503 prep into,incorporated
R2162 T8505 T8506 det the,locus
R2163 T8506 T8504 pobj locus,into
R2164 T8507 T8506 compound Fog2,locus
R2165 T8508 T8509 punct (,S.
R2166 T8509 T8495 meta S.,evaluated
R2167 T8510 T8509 nmod Tevosian,S.
R2168 T8511 T8509 punct ", ",S.
R2169 T8512 T8509 amod unpublished,S.
R2170 T8513 T8509 nmod data,S.
R2171 T8514 T8509 punct ),S.
R2172 T8515 T8495 punct .,evaluated
R2173 T8517 T8518 prep In,showed
R2174 T8519 T8520 nummod nine,mice
R2175 T8520 T8517 pobj mice,In
R2176 T8521 T8520 acl examined,mice
R2177 T8522 T8521 prep at,examined
R2178 T8523 T8522 pobj E11.5,at
R2179 T8524 T8518 punct ", ",showed
R2180 T8525 T8526 det all,lungs
R2181 T8526 T8518 nsubj lungs,showed
R2182 T8527 T8528 det a,enhancement
R2183 T8528 T8518 dobj enhancement,showed
R2184 T8529 T8528 amod specific,enhancement
R2185 T8530 T8528 prep of,enhancement
R2186 T8531 T8532 compound Fog2,expression
R2187 T8532 T8530 pobj expression,of
R2188 T8533 T8518 prep in,showed
R2189 T8534 T8535 det the,mesenchyme
R2190 T8535 T8533 pobj mesenchyme,in
R2191 T8536 T8535 acl surrounding,mesenchyme
R2192 T8537 T8538 det the,bud
R2193 T8538 T8536 dobj bud,surrounding
R2194 T8539 T8538 amod accessory,bud
R2195 T8540 T8538 cc and,bud
R2196 T8541 T8542 det the,bud
R2197 T8542 T8538 conj bud,bud
R2198 T8543 T8542 amod right,bud
R2199 T8544 T8542 amod middle,bud
R2200 T8545 T8542 compound lobe,bud
R2201 T8546 T8538 punct ", ",bud
R2202 T8547 T8548 dep which,are
R2203 T8548 T8538 relcl are,bud
R2204 T8549 T8550 det the,lobes
R2205 T8550 T8548 attr lobes,are
R2206 T8551 T8552 dep that,develop
R2207 T8552 T8550 relcl develop,lobes
R2208 T8553 T8552 aux do,develop
R2209 T8554 T8552 neg not,develop
R2210 T8555 T8552 advmod normally,develop
R2211 T8556 T8552 prep in,develop
R2212 T8557 T8558 compound Fog2,mice
R2213 T8558 T8556 pobj mice,in
R2214 T8559 T8558 compound mutant,mice
R2215 T8560 T8561 punct (,Figure
R2216 T8561 T8518 parataxis Figure,showed
R2217 T8562 T8561 nummod 5,Figure
R2218 T8563 T8561 punct ),Figure
R2219 T8564 T8518 punct .,showed
R2220 T8566 T8567 prep By,was
R2221 T8568 T8566 pobj E12.5,By
R2222 T8569 T8567 punct ", ",was
R2223 T8570 T8567 nsubj expression,was
R2224 T8571 T8567 acomp diffuse,was
R2225 T8572 T8567 prep in,was
R2226 T8573 T8574 det the,mesenchyme
R2227 T8574 T8572 pobj mesenchyme,in
R2228 T8575 T8574 amod pulmonary,mesenchyme
R2229 T8576 T8567 punct ", ",was
R2230 T8577 T8578 mark as,seen
R2231 T8578 T8567 advcl seen,was
R2232 T8579 T8578 auxpass was,seen
R2233 T8580 T8578 advmod previously,seen
R2234 T8581 T8578 prep with,seen
R2235 T8582 T8583 advmod in,situ
R2236 T8583 T8584 amod situ,hybridization
R2237 T8584 T8581 pobj hybridization,with
R2238 T8585 T8584 prep on,hybridization
R2239 T8586 T8587 compound tissue,sections
R2240 T8587 T8585 pobj sections,on
R2241 T8588 T8567 punct .,was
R2242 T8590 T8591 nsubj Diaphragms,show
R2243 T8592 T8590 prep from,Diaphragms
R2244 T8593 T8594 compound Fog2,mice
R2245 T8594 T8592 pobj mice,from
R2246 T8595 T8594 compound lil,mice
R2247 T8596 T8597 det an,membrane
R2248 T8597 T8591 dobj membrane,show
R2249 T8598 T8597 amod intact,membrane
R2250 T8599 T8597 prep with,membrane
R2251 T8600 T8601 det a,defect
R2252 T8601 T8599 pobj defect,with
R2253 T8602 T8601 prep in,defect
R2254 T8603 T8604 amod muscular,patterning
R2255 T8604 T8602 pobj patterning,in
R2256 T8605 T8606 punct (,see
R2257 T8606 T8591 parataxis see,show
R2258 T8607 T8606 dobj Figure,see
R2259 T8608 T8607 nummod 1B,Figure
R2260 T8609 T8606 punct ),see
R2261 T8610 T8591 punct .,show
R2262 T8612 T8613 det The,portion
R2263 T8613 T8615 nsubjpass portion,populated
R2264 T8614 T8613 amod membranous,portion
R2265 T8658 T8656 cc but,intact
R2266 T8659 T8656 conj amuscular,intact
R2267 T8616 T8613 prep of,portion
R2268 T8660 T8661 punct [,22
R2269 T8661 T8641 parataxis 22,have
R2270 T8662 T8661 nummod 17,22
R2271 T8617 T8618 det the,diaphragm
R2272 T8663 T8661 punct ",",22
R2273 T8664 T8661 nummod 21,22
R2274 T8665 T8661 punct ",",22
R2275 T8618 T8616 pobj diaphragm,of
R2276 T8666 T8661 punct ],22
R2277 T8667 T8641 punct .,have
R2278 T8619 T8615 auxpass is,populated
R2279 T8669 T8670 compound Hepatocyte,factor
R2280 T8670 T8672 nsubj factor,is
R2281 T8620 T8615 agent by,populated
R2282 T8671 T8670 compound growth,factor
R2283 T8673 T8670 punct /,factor
R2284 T8621 T8622 det a,population
R2285 T8674 T8675 compound Scatter,factor
R2286 T8675 T8670 appos factor,factor
R2287 T8676 T8670 punct (,factor
R2288 T8622 T8620 pobj population,by
R2289 T8677 T8670 appos HGF,factor
R2290 T8678 T8672 punct ),is
R2291 T8679 T8680 nummod one,candidate
R2292 T8623 T8622 amod migratory,population
R2293 T8680 T8672 attr candidate,is
R2294 T8681 T8680 amod potential,candidate
R2295 T8624 T8622 prep of,population
R2296 T8682 T8680 amod responsible,candidate
R2297 T8683 T8682 prep for,responsible
R2298 T8684 T8685 det the,guidance
R2299 T8625 T8626 compound muscle,cells
R2300 T8685 T8683 pobj guidance,for
R2301 T8686 T8685 prep of,guidance
R2302 T8687 T8688 compound muscle,precursors
R2303 T8626 T8624 pobj cells,of
R2304 T8688 T8686 pobj precursors,of
R2305 T8689 T8688 prep to,precursors
R2306 T8627 T8626 compound precursor,cells
R2307 T8690 T8691 det the,diaphragm
R2308 T8691 T8689 pobj diaphragm,to
R2309 T8692 T8691 amod membranous,diaphragm
R2310 T8628 T8615 punct ", ",populated
R2311 T8693 T8672 punct .,is
R2312 T8629 T8630 advmod much,like
R2313 T8695 T8696 nsubjpass It,shown
R2314 T8697 T8696 aux has,shown
R2315 T8630 T8615 prep like,populated
R2316 T8698 T8696 auxpass been,shown
R2317 T8631 T8632 det the,limbs
R2318 T8699 T8700 mark that,expressed
R2319 T8700 T8696 ccomp expressed,shown
R2320 T8701 T8700 nsubjpass HGF,expressed
R2321 T8702 T8700 auxpass is,expressed
R2322 T8632 T8630 pobj limbs,like
R2323 T8703 T8700 prep along,expressed
R2324 T8704 T8705 det this,pathway
R2325 T8633 T8634 punct [,20
R2326 T8705 T8703 pobj pathway,along
R2327 T8706 T8705 amod anatomic,pathway
R2328 T8707 T8708 punct [,23
R2329 T8634 T8615 parataxis 20,populated
R2330 T8708 T8696 parataxis 23,shown
R2331 T8709 T8708 punct ],23
R2332 T8635 T8634 nummod 19,20
R2333 T8710 T8696 punct ", ",shown
R2334 T8711 T8696 cc and,shown
R2335 T8712 T8713 nsubj mice,fail
R2336 T8636 T8634 punct ",",20
R2337 T8713 T8696 conj fail,shown
R2338 T8714 T8712 prep with,mice
R2339 T8637 T8634 punct ],20
R2340 T8715 T8714 pobj absence,with
R2341 T8716 T8715 prep of,absence
R2342 T8717 T8718 det the,receptor
R2343 T8638 T8615 punct .,populated
R2344 T8718 T8716 pobj receptor,of
R2345 T8719 T8718 compound HGF,receptor
R2346 T8720 T8721 compound c,Met
R2347 T8640 T8641 nsubj Mice,have
R2348 T8721 T8718 appos Met,receptor
R2349 T8722 T8721 punct -,Met
R2350 T8723 T8724 aux to,form
R2351 T8724 T8713 xcomp form,fail
R2352 T8725 T8726 amod muscularized,folds
R2353 T8642 T8640 prep with,Mice
R2354 T8726 T8724 dobj folds,form
R2355 T8727 T8726 amod pleuroperitoneal,folds
R2356 T8728 T8726 punct (,folds
R2357 T8729 T8726 appos PPFs,folds
R2358 T8643 T8642 pobj defects,with
R2359 T8730 T8713 punct ),fail
R2360 T8644 T8643 prep in,defects
R2361 T8731 T8713 punct ", ",fail
R2362 T8732 T8713 cc and,fail
R2363 T8733 T8734 advmod thus,have
R2364 T8645 T8644 pobj genes,in
R2365 T8734 T8713 conj have,fail
R2366 T8735 T8736 amod amuscular,diaphragms
R2367 T8736 T8734 dobj diaphragms,have
R2368 T8646 T8645 acl known,genes
R2369 T8737 T8738 punct [,25
R237 T2580 T2581 amod Congenital,defects
R2370 T8738 T8734 parataxis 25,have
R2371 T8739 T8738 nummod 24,25
R2372 T8740 T8738 punct ",",25
R2373 T8741 T8738 punct ],25
R2374 T8742 T8713 punct .,fail
R2375 T8647 T8648 aux to,be
R2376 T8744 T8745 nsubjpass Fog2,expressed
R2377 T8746 T8745 auxpass is,expressed
R2378 T8648 T8646 xcomp be,known
R2379 T8747 T8745 advmod diffusely,expressed
R238 T2581 T2583 nsubj defects,are
R2380 T8748 T8745 prep in,expressed
R2381 T8649 T8648 acomp important,be
R2382 T8650 T8649 prep for,important
R2383 T8749 T8750 det the,diaphragm
R2384 T8651 T8652 det the,control
R2385 T8750 T8748 pobj diaphragm,in
R2386 T8751 T8750 amod early,diaphragm
R2387 T8652 T8650 pobj control,for
R2388 T8752 T8750 amod amuscular,diaphragm
R2389 T8753 T8750 prep at,diaphragm
R239 T2582 T2581 amod diaphragmatic,defects
R2390 T8653 T8652 prep of,control
R2391 T8754 T8753 pobj E11.5,at
R2392 T8755 T8756 advmod as,as
R2393 T8756 T8748 cc as,in
R2394 T8654 T8655 det this,process
R2395 T8757 T8756 advmod well,as
R2396 T8758 T8748 conj in,in
R2397 T8759 T8760 det the,diaphragm
R2398 T8655 T8653 pobj process,of
R2399 T8760 T8758 pobj diaphragm,in
R240 T2584 T2585 det a,spectrum
R2400 T8761 T8760 amod later,diaphragm
R2401 T8762 T8760 amod muscularized,diaphragm
R2402 T8656 T8657 amod intact,diaphragms
R2403 T8657 T8641 dobj diaphragms,have
R2404 T8869 T8867 pobj path,along
R2405 T8763 T8764 punct (,see
R2406 T8870 T8869 prep of,path
R2407 T8764 T8745 parataxis see,expressed
R2408 T8765 T8766 nmod Figure,3C
R2409 T8871 T8872 compound muscle,cell
R241 T2585 T2583 attr spectrum,are
R2410 T8766 T8764 dobj 3C,see
R2411 T8767 T8766 cc and,3C
R2412 T8768 T8766 conj 3D,3C
R2413 T8872 T8874 compound cell,migration
R2414 T8769 T8764 punct ),see
R2415 T8770 T8745 punct .,expressed
R2416 T8873 T8872 compound precursor,cell
R2417 T8772 T8773 nsubjpass Pax3,detected
R2418 T8874 T8870 pobj migration,of
R2419 T8774 T8772 cc and,Pax3
R242 T2586 T2585 prep of,spectrum
R2420 T8775 T8772 conj MyoD,Pax3
R2421 T8776 T8772 punct ", ",Pax3
R2422 T8777 T8778 compound transcription,factors
R2423 T8778 T8772 appos factors,Pax3
R2424 T8779 T8778 acl required,factors
R2425 T8875 T8869 prep between,path
R2426 T8780 T8779 prep for,required
R2427 T8781 T8782 amod appropriate,migration
R2428 T8782 T8780 pobj migration,for
R2429 T8783 T8782 cc and,migration
R243 T2587 T2588 advmod relatively,common
R2430 T8876 T8877 det the,PPF
R2431 T8877 T8875 pobj PPF,between
R2432 T8784 T8782 conj determination,migration
R2433 T8878 T8877 cc and,PPF
R2434 T8785 T8782 prep of,migration
R2435 T8786 T8787 amod myogenic,precursors
R2436 T8787 T8785 pobj precursors,of
R2437 T8879 T8880 det the,diaphragm
R2438 T8788 T8773 punct ", ",detected
R2439 T8789 T8773 auxpass were,detected
R244 T2588 T2589 amod common,defects
R2440 T8790 T8773 prep in,detected
R2441 T8880 T8877 conj diaphragm,PPF
R2442 T8791 T8792 det the,PPFs
R2443 T8792 T8790 pobj PPFs,in
R2444 T8881 T8863 prep for,accounts
R2445 T8793 T8792 prep of,PPFs
R2446 T8794 T8795 compound Fog2,mice
R2447 T8795 T8793 pobj mice,of
R2448 T8796 T8795 compound lil,mice
R2449 T8882 T8883 det the,phenotype
R245 T2589 T2586 pobj defects,of
R2450 T8797 T8798 punct (,shown
R2451 T8798 T8773 parataxis shown,detected
R2452 T8799 T8798 nsubj data,shown
R2453 T8883 T8881 pobj phenotype,for
R2454 T8800 T8798 neg not,shown
R2455 T8801 T8798 punct ),shown
R2456 T8884 T8883 amod abnormal,phenotype
R2457 T8802 T8773 punct .,detected
R2458 T8804 T8805 advmod However,demonstrated
R2459 T8885 T8863 prep in,accounts
R246 T2590 T2589 compound birth,defects
R2460 T8806 T8805 punct ", ",demonstrated
R2461 T8807 T8808 advmod in,situ
R2462 T8886 T8887 det these,mice
R2463 T8808 T8809 amod situ,analysis
R2464 T8809 T8805 nsubj analysis,demonstrated
R2465 T8810 T8809 compound expression,analysis
R2466 T8887 T8885 pobj mice,in
R2467 T8811 T8812 mark that,reduced
R2468 T8812 T8805 ccomp reduced,demonstrated
R2469 T8813 T8814 det the,expression
R247 T2591 T2583 punct .,are
R2470 T8888 T8863 punct .,accounts
R2471 T8814 T8812 nsubjpass expression,reduced
R2472 T8815 T8814 prep of,expression
R2473 T8816 T8815 pobj HGF,of
R2474 T8817 T8814 prep in,expression
R2475 T8818 T8819 det the,region
R2476 T8819 T8817 pobj region,in
R2477 T8820 T8821 advmod where,meets
R2478 T8821 T8819 relcl meets,region
R2479 T8822 T8823 det this,structure
R248 T2593 T2594 det The,Bochdalek
R2480 T8823 T8821 nsubj structure,meets
R2481 T8824 T8825 det the,diaphragm
R2482 T8825 T8821 dobj diaphragm,meets
R2483 T8826 T8825 amod membranous,diaphragm
R2484 T8827 T8812 auxpass was,reduced
R2485 T8828 T8812 advmod markedly,reduced
R2486 T8829 T8812 prep in,reduced
R2487 T8830 T8831 compound Fog2,mice
R2488 T8831 T8829 pobj mice,in
R2489 T8832 T8831 compound mutant,mice
R249 T2594 T2595 nsubj Bochdalek,occur
R2490 T8833 T8834 punct (,Figure
R2491 T8834 T8805 parataxis Figure,demonstrated
R2492 T8835 T8834 nummod 6,Figure
R2493 T8836 T8834 punct ),Figure
R2494 T8837 T8805 punct .,demonstrated
R2495 T8839 T8840 nsubj We,hypothesize
R2496 T8841 T8842 mark that,required
R2497 T8842 T8840 ccomp required,hypothesize
R2498 T8843 T8842 nsubjpass Fog2,required
R2499 T8844 T8842 auxpass is,required
R250 T2596 T2594 cc or,Bochdalek
R2500 T8845 T8842 punct (,required
R2501 T8846 T8847 preconj either,directly
R2502 T8847 T8842 advmod directly,required
R2503 T8848 T8847 cc or,directly
R2504 T8849 T8847 conj indirectly,directly
R2505 T8850 T8842 punct ),required
R2506 T8851 T8842 prep for,required
R2507 T8852 T8853 det the,induction
R2508 T8853 T8851 pobj induction,for
R2509 T8854 T8853 prep of,induction
R251 T2597 T2598 amod posterolateral,hernias
R2510 T8855 T8854 pobj HGF,of
R2511 T8856 T8853 prep in,induction
R2512 T8857 T8858 det the,diaphragm
R2513 T8858 T8856 pobj diaphragm,in
R2514 T8859 T8858 amod developing,diaphragm
R2515 T8860 T8840 punct ", ",hypothesize
R2516 T8861 T8840 cc and,hypothesize
R2517 T8862 T8863 nsubj dysregulation,accounts
R2518 T8863 T8840 conj accounts,hypothesize
R2519 T8864 T8862 prep of,dysregulation
R252 T2598 T2594 conj hernias,Bochdalek
R2520 T8865 T8866 compound HGF,patterning
R2521 T8866 T8864 pobj patterning,of
R2522 T8867 T8866 prep along,patterning
R2523 T8868 T8869 det the,path
R2526 T9907 T9908 compound FOG2,Mutation
R2527 T9909 T9908 prep in,Mutation
R2528 T9910 T9911 det a,Patient
R2529 T9911 T9909 pobj Patient,in
R253 T2599 T2594 punct (,Bochdalek
R2530 T9912 T9911 prep with,Patient
R2531 T9913 T9914 nmod Diaphragm,Abnormalities
R2532 T9914 T9912 pobj Abnormalities,with
R2533 T9915 T9913 cc and,Diaphragm
R2534 T9916 T9913 conj Lung,Diaphragm
R2535 T9918 T9919 compound FOG2,analysis
R2536 T9919 T9921 nsubjpass analysis,performed
R2537 T9920 T9919 compound sequence,analysis
R2538 T9922 T9921 auxpass was,performed
R2539 T9923 T9921 prep on,performed
R254 T2600 T2601 advmod often,referred
R2540 T9924 T9925 compound autopsy,tissue
R2541 T9925 T9923 pobj tissue,on
R2542 T9926 T9925 prep from,tissue
R2543 T9927 T9928 quantmod 30,32
R2544 T9928 T9930 nummod 32,children
R2545 T9929 T9928 quantmod of,32
R2546 T9930 T9926 pobj children,from
R2547 T9931 T9930 amod deceased,children
R2548 T9932 T9930 prep with,children
R2549 T9933 T9934 det an,diagnosis
R255 T2601 T2594 acl referred,Bochdalek
R2550 T9934 T9932 pobj diagnosis,with
R2551 T9935 T9934 amod anatomic,diagnosis
R2552 T9936 T9934 prep of,diagnosis
R2553 T9937 T9938 amod diaphragmatic,defect
R2554 T9938 T9936 pobj defect,of
R2555 T9939 T9925 acl evaluated,tissue
R2556 T9940 T9939 prep at,evaluated
R2557 T9941 T9942 det the,Hospital
R2558 T9942 T9940 pobj Hospital,at
R2559 T9943 T9942 poss Children,Hospital
R256 T2602 T2601 prep to,referred
R2560 T9944 T9943 case 's,Children
R2561 T9945 T9942 prep in,Hospital
R2562 T9946 T9945 pobj Boston,in
R2563 T9947 T9946 punct ", ",Boston
R2564 T9948 T9946 npadvmod Massachusetts,Boston
R2565 T9949 T9939 punct ", ",evaluated
R2566 T9950 T9939 prep between,evaluated
R2567 T9951 T9950 pobj 1993,between
R2568 T9952 T9951 cc and,1993
R2569 T9953 T9951 conj 2003,1993
R257 T2603 T2601 prep as,referred
R2570 T9954 T9921 punct .,performed
R2571 T9956 T9957 compound Autopsy,reports
R2572 T9957 T9958 nsubjpass reports,reviewed
R2573 T9959 T9958 auxpass were,reviewed
R2574 T9960 T9961 aux to,determine
R2575 T9961 T9958 advcl determine,reviewed
R2576 T9962 T9963 det the,diagnoses
R2577 T9963 T9961 dobj diagnoses,determine
R2578 T9964 T9963 amod specific,diagnoses
R2579 T9965 T9958 punct .,reviewed
R258 T2604 T2605 amod congenital,hernia
R2580 T9967 T9968 prep Of,had
R2581 T9969 T9970 det these,cases
R2582 T9970 T9967 pobj cases,Of
R2583 T9971 T9970 nummod 30,cases
R2584 T9972 T9968 punct ", ",had
R2585 T9973 T9968 nsubj 17,had
R2586 T9974 T9975 punct (,%
R2587 T9975 T9973 parataxis %,17
R2588 T9976 T9975 nummod 57,%
R2589 T9977 T9975 punct ),%
R259 T2605 T2603 pobj hernia,as
R2590 T9978 T9979 compound Bochdalek,CDH
R2591 T9979 T9968 dobj CDH,had
R2592 T9980 T9968 punct ", ",had
R2593 T9981 T9982 nsubj two,had
R2594 T9982 T9968 conj had,had
R2595 T9983 T9984 punct (,%
R2596 T9984 T9981 parataxis %,two
R2597 T9985 T9984 nummod 7,%
R2598 T9986 T9984 punct ),%
R2599 T9987 T9988 amod diaphragmatic,agenesis
R260 T2606 T2605 amod diaphragmatic,hernia
R2600 T9988 T9982 dobj agenesis,had
R2601 T9989 T9982 punct ", ",had
R2602 T9990 T9991 nsubj seven,had
R2603 T9991 T9982 conj had,had
R2604 T9992 T9993 punct (,%
R2605 T9993 T9990 parataxis %,seven
R2606 T9994 T9993 nummod 23,%
R2607 T9995 T9993 punct ),%
R2608 T9996 T9997 amod diaphragmatic,eventration
R2609 T9997 T9991 dobj eventration,had
R261 T2607 T2605 punct [,hernia
R2610 T9998 T9997 punct /,eventration
R2611 T9999 T10000 compound muscularization,defects
R2612 T10000 T9997 appos defects,eventration
R2613 T10001 T9997 punct (,eventration
R2614 T10002 T9997 prep without,eventration
R2615 T10003 T10004 compound Bochdalek,CDH
R2616 T10004 T10002 pobj CDH,without
R2617 T10005 T9991 punct ),had
R2618 T10006 T9991 punct ", ",had
R2619 T10007 T9991 cc and,had
R262 T2608 T2605 appos CDH,hernia
R2620 T10008 T10009 nsubj four,had
R2621 T10009 T9991 conj had,had
R2622 T10010 T10011 punct (,%
R2623 T10011 T10008 parataxis %,four
R2624 T10012 T10011 nummod 13,%
R2625 T10013 T10011 punct ),%
R2626 T10014 T10015 compound Bochdalek,hernia
R2627 T10015 T10009 dobj hernia,had
R2628 T10016 T10015 prep of,hernia
R2629 T10017 T10018 nummod one,hemidiaphragm
R263 T2609 T2594 punct ],Bochdalek
R2630 T10018 T10016 pobj hemidiaphragm,of
R2631 T10019 T10015 cc and,hernia
R2632 T10020 T10015 conj eventration,hernia
R2633 T10021 T10020 prep of,eventration
R2634 T10022 T10023 det the,other
R2635 T10023 T10021 pobj other,of
R2636 T10024 T10009 punct .,had
R2637 T10026 T10027 amod Pulmonary,hypoplasia
R2638 T10027 T10028 nsubjpass hypoplasia,assessed
R2639 T10029 T10028 auxpass was,assessed
R264 T2610 T2594 punct ),Bochdalek
R2640 T10030 T10028 advcl using,assessed
R2641 T10031 T10032 compound lung,body
R2642 T10032 T10034 compound body,ratio
R2643 T10033 T10032 punct /,body
R2644 T10034 T10030 dobj ratio,using
R2645 T10035 T10034 compound weight,ratio
R2646 T10036 T10034 cc and,ratio
R2647 T10037 T10038 amod radial,counts
R2648 T10038 T10034 conj counts,ratio
R2649 T10039 T10038 amod alveolar,counts
R265 T2611 T2595 prep in,occur
R2650 T10040 T10041 punct [,26
R2651 T10041 T10028 parataxis 26,assessed
R2652 T10042 T10041 punct ],26
R2653 T10043 T10028 punct .,assessed
R2654 T10045 T10046 det The,material
R2655 T10046 T10047 nsubj material,included
R2656 T10048 T10046 amod available,material
R2657 T10049 T10048 prep for,available
R2658 T10050 T10049 pobj review,for
R2659 T10051 T10052 amod written,reports
R266 T2612 T2613 quantmod 1,"3,000"
R2660 T10052 T10047 dobj reports,included
R2661 T10053 T10052 cc and,reports
R2662 T10054 T10055 amod histologic,slides
R2663 T10055 T10052 conj slides,reports
R2664 T10056 T10047 prep in,included
R2665 T10057 T10058 det all,cases
R2666 T10058 T10056 pobj cases,in
R2667 T10059 T10047 cc and,included
R2668 T10060 T10061 amod gross,kodachromes
R2669 T10061 T10047 conj kodachromes,included
R267 T2613 T2615 nummod "3,000",births
R2670 T10062 T10061 prep in,kodachromes
R2671 T10063 T10064 det a,subset
R2672 T10064 T10062 pobj subset,in
R2673 T10065 T10064 prep of,subset
R2674 T10066 T10065 pobj cases,of
R2675 T10067 T10047 punct .,included
R2676 T10069 T10070 nummod One,child
R2677 T10070 T10071 nsubj child,carried
R2678 T10072 T10073 det a,change
R2679 T10073 T10071 dobj change,carried
R268 T2614 T2613 punct /,"3,000"
R2680 T10074 T10075 advmod highly,significant
R2681 T10075 T10073 amod significant,change
R2682 T10076 T10073 compound FOG2,change
R2683 T10077 T10073 compound sequence,change
R2684 T10078 T10071 punct .,carried
R2685 T10080 T10081 det The,patient
R2686 T10081 T10082 nsubj patient,was
R2687 T10083 T10084 det a,girl
R2688 T10084 T10082 attr girl,was
R2689 T10085 T10086 amod full,term
R269 T2615 T2611 pobj births,in
R2690 T10086 T10084 nmod term,girl
R2691 T10087 T10086 punct -,term
R2692 T10088 T10089 nummod "3,500",g
R2693 T10089 T10084 compound g,girl
R2694 T10090 T10089 punct -,g
R2695 T10091 T10084 compound baby,girl
R2696 T10092 T10093 dep who,developed
R2697 T10093 T10084 relcl developed,girl
R2698 T10094 T10095 amod severe,failure
R2699 T10095 T10093 dobj failure,developed
R270 T2616 T2615 amod live,births
R2700 T10096 T10095 amod respiratory,failure
R2701 T10097 T10093 prep at,developed
R2702 T10098 T10097 pobj birth,at
R2703 T10099 T10093 cc and,developed
R2704 T10100 T10093 conj died,developed
R2705 T10101 T10100 prep after,died
R2706 T10102 T10103 nummod 5,h
R2707 T10103 T10101 pobj h,after
R2708 T10104 T10103 prep of,h
R2709 T10105 T10104 pobj resuscitation,of
R271 T2617 T2618 punct [,1
R2710 T10106 T10082 punct .,was
R2711 T10108 T10109 compound Antemortem,radiographs
R2712 T10109 T10110 nsubj radiographs,showed
R2713 T10111 T10112 amod opacified,fields
R2714 T10112 T10110 dobj fields,showed
R2715 T10113 T10112 compound lung,fields
R2716 T10114 T10112 cc and,fields
R2717 T10115 T10116 amod possible,bowel
R2718 T10116 T10112 conj bowel,fields
R2719 T10117 T10116 prep in,bowel
R272 T2618 T2595 parataxis 1,occur
R2720 T10118 T10119 det the,chest
R2721 T10119 T10117 pobj chest,in
R2722 T10120 T10110 punct .,showed
R2723 T10122 T10123 det The,patient
R2724 T10123 T10124 poss patient,diagnosis
R2725 T10124 T10127 nsubj diagnosis,was
R2726 T10125 T10123 case 's,patient
R2727 T10126 T10124 amod clinical,diagnosis
R2728 T10128 T10127 attr CDH,was
R2729 T10129 T10127 punct .,was
R273 T2619 T2618 punct ],1
R2730 T10131 T10132 nsubj Review,revealed
R2731 T10133 T10131 prep of,Review
R2732 T10134 T10135 compound autopsy,material
R2733 T10135 T10133 pobj material,of
R2734 T10136 T10137 amod severe,hypoplasia
R2735 T10137 T10132 dobj hypoplasia,revealed
R2736 T10138 T10137 amod bilateral,hypoplasia
R2737 T10139 T10137 amod pulmonary,hypoplasia
R2738 T10140 T10141 punct (,27
R2739 T10141 T10137 parataxis 27,hypoplasia
R274 T2620 T2595 punct ", ",occur
R2740 T10142 T10143 amod combined,weight
R2741 T10143 T10145 dep weight,g
R2742 T10144 T10143 compound lung,weight
R2743 T10145 T10141 dep g,27
R2744 T10146 T10145 punct ", ",g
R2745 T10147 T10145 nummod 11.1,g
R2746 T10148 T10141 punct ;,27
R2747 T10149 T10141 dep expected,27
R2748 T10150 T10149 prep for,expected
R2749 T10151 T10152 compound body,weight
R275 T2621 T2595 cc and,occur
R2750 T10152 T10150 pobj weight,for
R2751 T10153 T10152 compound length,weight
R2752 T10154 T10152 punct /,weight
R2753 T10155 T10149 punct ", ",expected
R2754 T10156 T10157 quantmod 46.8,26.2
R2755 T10157 T10159 nummod 26.2,g
R2756 T10158 T10157 punct ±,26.2
R2757 T10159 T10149 dep g,expected
R2758 T10160 T10141 punct ;,27
R2759 T10161 T10141 punct [,27
R276 T2622 T2623 mark although,are
R2760 T10162 T10141 punct ],27
R2761 T10163 T10141 punct ),27
R2762 T10164 T10137 punct ", ",hypoplasia
R2763 T10165 T10166 advmod most,markedly
R2764 T10166 T10167 advmod markedly,involving
R2765 T10167 T10137 acl involving,hypoplasia
R2766 T10168 T10169 det the,lung
R2767 T10169 T10167 dobj lung,involving
R2768 T10170 T10169 amod left,lung
R2769 T10171 T10132 punct .,revealed
R277 T2623 T2625 advcl are,have
R2770 T10173 T10174 det The,ratio
R2771 T10174 T10179 nsubj ratio,was
R2772 T10175 T10174 nmod lung,ratio
R2773 T10176 T10177 punct /,body
R2774 T10177 T10175 prep body,lung
R2775 T10178 T10174 compound weight,ratio
R2776 T10180 T10179 attr 0.0037,was
R2777 T10181 T10182 punct (,0.010
R2778 T10182 T10179 parataxis 0.010,was
R2779 T10183 T10182 nsubj expected,0.010
R278 T2624 T2623 nsubj these,are
R2780 T10184 T10182 punct >,0.010
R2781 T10185 T10182 punct ),0.010
R2782 T10186 T10187 punct [,28
R2783 T10187 T10179 parataxis 28,was
R2784 T10188 T10187 punct ],28
R2785 T10189 T10179 punct .,was
R2786 T10191 T10192 expl There,were
R2787 T10193 T10194 det a,number
R2788 T10194 T10192 attr number,were
R2789 T10195 T10194 amod reduced,number
R279 T2625 T2595 conj have,occur
R2790 T10196 T10194 prep of,number
R2791 T10197 T10198 amod bronchial,generations
R2792 T10198 T10196 pobj generations,of
R2793 T10199 T10192 punct ", ",were
R2794 T10200 T10192 cc and,were
R2795 T10201 T10202 det the,count
R2796 T10202 T10205 nsubj count,was
R2797 T10203 T10202 amod radial,count
R2798 T10204 T10202 amod alveolar,count
R2799 T10205 T10192 conj was,were
R280 T2626 T2627 det the,type
R2800 T10206 T10207 quantmod 2,3
R2801 T10207 T10205 attr 3,was
R2802 T10208 T10207 punct –,3
R2803 T10209 T10210 punct (,5
R2804 T10210 T10205 parataxis 5,was
R2805 T10211 T10210 nsubj expected,5
R2806 T10212 T10210 punct =,5
R2807 T10213 T10210 punct ),5
R2808 T10214 T10215 punct [,29
R2809 T10215 T10205 parataxis 29,was
R281 T2627 T2623 attr type,are
R2810 T10216 T10215 punct ],29
R2811 T10217 T10205 punct .,was
R2812 T10219 T10220 expl There,were
R2813 T10221 T10222 amod incomplete,fissures
R2814 T10222 T10220 attr fissures,were
R2815 T10223 T10222 compound lung,fissures
R2816 T10224 T10220 advmod bilaterally,were
R2817 T10225 T10220 punct .,were
R2818 T10227 T10228 det A,eventration
R2819 T10228 T10232 nsubj eventration,was
R282 T2628 T2629 advmod most,common
R2820 T10229 T10228 amod deep,eventration
R2821 T10230 T10228 amod posterior,eventration
R2822 T10231 T10228 amod diaphragmatic,eventration
R2823 T10233 T10232 acomp present,was
R2824 T10234 T10232 prep on,was
R2825 T10235 T10236 det the,side
R2826 T10236 T10234 pobj side,on
R2827 T10237 T10236 amod left,side
R2828 T10238 T10232 punct .,was
R2829 T10240 T10241 advmod Additionally,encircled
R283 T2629 T2627 amod common,type
R2830 T10242 T10241 punct ", ",encircled
R2831 T10243 T10244 nummod two,bands
R2832 T10244 T10241 nsubj bands,encircled
R2833 T10245 T10244 amod muscularized,bands
R2834 T10246 T10244 amod ligamentous,bands
R2835 T10247 T10244 acl resembling,bands
R2836 T10248 T10249 amod diaphragmatic,remnants
R2837 T10249 T10247 dobj remnants,resembling
R2838 T10250 T10251 det the,lobe
R2839 T10251 T10241 dobj lobe,encircled
R284 T2630 T2627 prep of,type
R2840 T10252 T10251 amod left,lobe
R2841 T10253 T10251 prep of,lobe
R2842 T10254 T10255 det the,liver
R2843 T10255 T10253 pobj liver,of
R2844 T10256 T10241 punct ", ",encircled
R2845 T10257 T10241 advcl creating,encircled
R2846 T10258 T10259 det an,contour
R2847 T10259 T10257 dobj contour,creating
R2848 T10260 T10259 amod abnormal,contour
R2849 T10261 T10259 amod fissured,contour
R285 T2631 T2632 amod diaphragmatic,defect
R2850 T10262 T10259 compound liver,contour
R2851 T10263 T10241 punct .,encircled
R2852 T10265 T10266 advmod Away,appeared
R2853 T10267 T10265 prep from,Away
R2854 T10268 T10269 det the,eventration
R2855 T10269 T10267 pobj eventration,from
R2856 T10270 T10266 punct ", ",appeared
R2857 T10271 T10272 det the,diaphragm
R2858 T10272 T10266 nsubj diaphragm,appeared
R2859 T10273 T10274 advmod well,muscularized
R286 T2632 T2630 pobj defect,of
R2860 T10274 T10266 oprd muscularized,appeared
R2861 T10275 T10266 punct ", ",appeared
R2862 T10276 T10266 advcl measuring,appeared
R2863 T10277 T10278 nummod 0.3,cm
R2864 T10278 T10276 dobj cm,measuring
R2865 T10279 T10278 prep in,cm
R2866 T10280 T10279 pobj thickness,in
R2867 T10281 T10266 punct .,appeared
R2868 T10283 T10284 det A,autopsy
R2869 T10284 T10286 nsubj autopsy,revealed
R287 T2633 T2627 acl presenting,type
R2870 T10285 T10284 amod complete,autopsy
R2871 T10286 T10287 ccomp revealed,determined
R2872 T10288 T10289 det no,malformations
R2873 T10289 T10286 dobj malformations,revealed
R2874 T10290 T10289 amod other,malformations
R2875 T10291 T10287 punct ;,determined
R2876 T10292 T10293 det the,heart
R2877 T10293 T10287 nsubjpass heart,determined
R2878 T10294 T10287 auxpass was,determined
R2879 T10295 T10296 aux to,be
R288 T2634 T2633 prep at,presenting
R2880 T10296 T10287 xcomp be,determined
R2881 T10297 T10298 advmod grossly,normal
R2882 T10298 T10296 acomp normal,be
R2883 T10299 T10287 cc and,determined
R2884 T10300 T10301 auxpass was,donated
R2885 T10301 T10287 conj donated,determined
R2886 T10302 T10301 prep for,donated
R2887 T10303 T10304 compound valve,harvest
R2888 T10304 T10302 pobj harvest,for
R2889 T10305 T10287 punct .,determined
R289 T2635 T2634 pobj birth,at
R2890 T10307 T10308 compound Sequence,analysis
R2891 T10308 T10309 nsubj analysis,revealed
R2892 T10310 T10311 det a,change
R2893 T10311 T10309 dobj change,revealed
R2894 T10312 T10311 nmod cytosine,change
R2895 T10313 T10312 prep to,cytosine
R2896 T10314 T10313 pobj thymine,to
R2897 T10315 T10311 amod heterozygous,change
R2898 T10316 T10311 prep in,change
R2899 T10317 T10316 pobj exon,in
R290 T2636 T2625 punct ", ",have
R2900 T10318 T10317 nummod 4,exon
R2901 T10319 T10320 dep that,changes
R2902 T10320 T10311 relcl changes,change
R2903 T10321 T10322 det the,acid
R2904 T10322 T10320 dobj acid,changes
R2905 T10323 T10322 amod 112th,acid
R2906 T10324 T10322 compound amino,acid
R2907 T10325 T10320 prep from,changes
R2908 T10326 T10325 pobj arginine,from
R2909 T10327 T10320 prep to,changes
R291 T2637 T2638 amod diaphragmatic,aplasia
R2910 T10328 T10329 det a,codon
R2911 T10329 T10327 pobj codon,to
R2912 T10330 T10329 compound stop,codon
R2913 T10331 T10309 punct .,revealed
R2914 T10333 T10334 det This,mutation
R2915 T10334 T10335 nsubj mutation,produces
R2916 T10336 T10337 det a,peptide
R2917 T10337 T10335 dobj peptide,produces
R2918 T10338 T10339 advmod severely,truncated
R2919 T10339 T10337 amod truncated,peptide
R292 T2638 T2625 nsubj aplasia,have
R2920 T10340 T10341 dep that,contain
R2921 T10341 T10337 relcl contain,peptide
R2922 T10342 T10341 aux does,contain
R2923 T10343 T10341 neg not,contain
R2924 T10344 T10345 compound zinc,finger
R2925 T10345 T10346 compound finger,domains
R2926 T10346 T10341 dobj domains,contain
R2927 T10347 T10348 punct (,Figure
R2928 T10348 T10335 parataxis Figure,produces
R2929 T10349 T10348 nummod 7,Figure
R293 T2639 T2638 cc and,aplasia
R2930 T10350 T10348 punct ),Figure
R2931 T10351 T10335 punct .,produces
R2932 T10353 T10354 det This,change
R2933 T10354 T10356 nsubj change,was
R2934 T10355 T10354 compound base,change
R2935 T10357 T10356 neg not,was
R2936 T10358 T10356 acomp present,was
R2937 T10359 T10356 prep in,was
R2938 T10360 T10361 det the,analysis
R2939 T10361 T10359 pobj analysis,in
R294 T2640 T2641 amod diaphragmatic,defects
R2940 T10362 T10361 prep of,analysis
R2941 T10363 T10362 pobj DNA,of
R2942 T10364 T10363 prep from,DNA
R2943 T10365 T10366 nummod 400,adults
R2944 T10366 T10364 pobj adults,from
R2945 T10367 T10366 amod normal,adults
R2946 T10368 T10356 punct .,was
R2947 T10370 T10371 aux To,assess
R2948 T10371 T10372 advcl assess,examined
R2949 T10373 T10374 det the,likelihood
R295 T2641 T2638 conj defects,aplasia
R2950 T10374 T10371 dobj likelihood,assess
R2951 T10375 T10376 mark that,was
R2952 T10376 T10374 acl was,likelihood
R2953 T10377 T10378 det the,mutation
R2954 T10378 T10376 nsubj mutation,was
R2955 T10379 T10376 acomp causal,was
R2956 T10380 T10379 prep for,causal
R2957 T10381 T10382 det the,phenotype
R2958 T10382 T10380 pobj phenotype,for
R2959 T10383 T10382 amod developmental,phenotype
R296 T2642 T2641 compound muscularization,defects
R2960 T10384 T10372 punct ", ",examined
R2961 T10385 T10372 nsubj we,examined
R2962 T10386 T10387 det both,parents
R2963 T10387 T10372 dobj parents,examined
R2964 T10388 T10372 punct .,examined
R2965 T10390 T10391 nsubjpass Paternity,confirmed
R2966 T10392 T10391 auxpass was,confirmed
R2967 T10393 T10391 punct ", ",confirmed
R2968 T10394 T10391 cc and,confirmed
R2969 T10395 T10396 compound sequence,analysis
R297 T2643 T2644 punct (,eventrations
R2970 T10396 T10397 nsubj analysis,revealed
R2971 T10397 T10391 conj revealed,confirmed
R2972 T10398 T10399 mark that,carried
R2973 T10399 T10397 ccomp carried,revealed
R2974 T10400 T10401 det the,parent
R2975 T10401 T10399 nsubj parent,carried
R2976 T10402 T10401 preconj neither,parent
R2977 T10403 T10404 det this,mutation
R2978 T10404 T10399 dobj mutation,carried
R2979 T10405 T10397 punct ", ",revealed
R298 T2644 T2641 parataxis eventrations,defects
R2980 T10406 T10397 advcl proving,revealed
R2981 T10407 T10408 mark that,had
R2982 T10408 T10406 ccomp had,proving
R2983 T10409 T10410 det the,patient
R2984 T10410 T10408 nsubj patient,had
R2985 T10411 T10412 det a,mutation
R2986 T10412 T10408 dobj mutation,had
R2987 T10413 T10414 advmod de,novo
R2988 T10414 T10412 amod novo,mutation
R2989 T10415 T10408 prep in,had
R299 T2645 T2644 punct ),eventrations
R2990 T10416 T10415 pobj FOG2,in
R2991 T10417 T10418 punct (,Figure
R2992 T10418 T10397 parataxis Figure,revealed
R2993 T10419 T10418 nummod 7,Figure
R2994 T10420 T10418 punct ),Figure
R2995 T10421 T10397 punct .,revealed
R300 T2646 T2625 aux may,have
R3008 T13037 T13038 amod Congenital,defects
R3009 T13038 T13040 nsubj defects,are
R301 T2647 T2648 det a,presentation
R3010 T13039 T13038 amod diaphragmatic,defects
R3011 T13041 T13042 det a,group
R3012 T13042 T13040 attr group,are
R3013 T13043 T13042 amod heterogeneous,group
R3014 T13044 T13042 prep of,group
R3015 T13045 T13044 pobj disorders,of
R3016 T13046 T13045 prep of,disorders
R3017 T13047 T13048 amod unknown,etiology
R3018 T13048 T13046 pobj etiology,of
R3019 T13049 T13040 punct .,are
R302 T2648 T2625 dobj presentation,have
R3020 T13051 T13052 det The,defects
R3021 T13052 T13053 nsubj defects,include
R3022 T13054 T13055 dep that,present
R3023 T13055 T13052 relcl present,defects
R3024 T13056 T13055 prep in,present
R3025 T13057 T13058 det the,period
R3026 T13058 T13056 pobj period,in
R3027 T13059 T13060 advmod pre,perinatal
R3028 T13060 T13058 amod perinatal,period
R3029 T13061 T13060 punct -,perinatal
R303 T2649 T2648 amod similar,presentation
R3030 T13062 T13060 cc or,perinatal
R3031 T13063 T13064 compound Bochdalek,hernia
R3032 T13064 T13053 dobj hernia,include
R3033 T13065 T13064 punct ", ",hernia
R3034 T13066 T13067 amod diaphragmatic,aplasia
R3035 T13067 T13064 conj aplasia,hernia
R3036 T13068 T13067 punct ", ",aplasia
R3037 T13069 T13067 cc and,aplasia
R3038 T13070 T13071 amod various,degrees
R3039 T13071 T13067 conj degrees,aplasia
R304 T2650 T2648 amod clinical,presentation
R3040 T13072 T13071 prep of,degrees
R3041 T13073 T13074 compound muscularization,defects
R3042 T13074 T13072 pobj defects,of
R3043 T13075 T13074 cc or,defects
R3044 T13076 T13074 conj eventrations,defects
R3045 T13077 T13053 punct .,include
R3046 T13079 T13080 amod Different,types
R3047 T13080 T13081 nsubj types,occur
R3048 T13082 T13080 prep of,types
R3049 T13083 T13082 pobj defects,of
R305 T2651 T2625 punct .,have
R3050 T13084 T13081 prep in,occur
R3051 T13085 T13086 det the,patients
R3052 T13086 T13084 pobj patients,in
R3053 T13087 T13086 amod same,patients
R3054 T13088 T13084 cc or,in
R3055 T13089 T13084 conj in,in
R3056 T13090 T13089 pobj siblings,in
R3057 T13091 T13081 punct ", ",occur
R3058 T13092 T13081 advcl suggesting,occur
R3059 T13093 T13094 nsubj these,represent
R306 T2653 T2654 csubj Making,be
R3060 T13094 T13092 advcl represent,suggesting
R3061 T13095 T13096 amod variable,expression
R3062 T13096 T13094 dobj expression,represent
R3063 T13097 T13096 prep of,expression
R3064 T13098 T13099 det the,pathogenesis
R3065 T13099 T13097 pobj pathogenesis,of
R3066 T13100 T13099 amod same,pathogenesis
R3067 T13101 T13099 amod underlying,pathogenesis
R3068 T13102 T13103 punct [,31
R3069 T13103 T13081 parataxis 31,occur
R307 T2655 T2656 amod specific,distinctions
R3070 T13104 T13103 nummod 30,31
R3071 T13105 T13103 punct ",",31
R3072 T13106 T13103 punct ],31
R3073 T13107 T13081 punct .,occur
R3074 T13109 T13110 amod Clinical,differentiation
R3075 T13110 T13111 nsubj differentiation,be
R3076 T13112 T13110 prep between,differentiation
R3077 T13113 T13114 det these,defects
R3078 T13114 T13112 pobj defects,between
R3079 T13115 T13111 aux may,be
R308 T2656 T2653 dobj distinctions,Making
R3080 T13116 T13117 advmod very,difficult
R3081 T13117 T13111 acomp difficult,be
R3082 T13118 T13111 punct ", ",be
R3083 T13119 T13120 mark as,is
R3084 T13120 T13111 advcl is,be
R3085 T13121 T13122 det the,diaphragm
R3086 T13122 T13120 nsubj diaphragm,is
R3087 T13123 T13122 amod residual,diaphragm
R3088 T13124 T13122 amod membranous,diaphragm
R3089 T13125 T13122 prep of,diaphragm
R309 T2657 T2656 amod anatomic,distinctions
R3090 T13126 T13127 det a,defect
R3091 T13127 T13125 pobj defect,of
R3092 T13128 T13127 compound muscularization,defect
R3093 T13129 T13120 acomp thin,is
R3094 T13130 T13120 cc and,is
R3095 T13131 T13132 aux may,be
R3096 T13132 T13120 conj be,is
R3097 T13133 T13132 neg not,be
R3098 T13134 T13135 advmod easily,visible
R3099 T13135 T13132 acomp visible,be
R310 T2658 T2653 prep among,Making
R3100 T13136 T13132 prep on,be
R3101 T13137 T13138 amod prenatal,ultrasound
R3102 T13138 T13136 pobj ultrasound,on
R3103 T13139 T13138 cc or,ultrasound
R3104 T13140 T13141 amod postnatal,radiographs
R3105 T13141 T13138 conj radiographs,ultrasound
R3106 T13142 T13141 compound chest,radiographs
R3107 T13143 T13144 punct [,32
R3108 T13144 T13111 parataxis 32,be
R3109 T13145 T13144 punct ],32
R311 T2659 T2660 det these,types
R3110 T13146 T13111 punct .,be
R3111 T13148 T13149 mark Although,considered
R3112 T13149 T13155 advcl considered,been
R3113 T13150 T13151 amod diaphragmatic,defects
R3114 T13151 T13149 nsubjpass defects,considered
R3115 T13152 T13151 compound muscularization,defects
R3116 T13153 T13149 auxpass were,considered
R3117 T13154 T13149 advmod historically,considered
R3118 T13156 T13157 aux to,be
R3119 T13157 T13149 xcomp be,considered
R312 T2660 T2658 pobj types,among
R3120 T13158 T13157 acomp predictive,be
R3121 T13159 T13158 prep of,predictive
R3122 T13160 T13161 det a,outcome
R3123 T13161 T13159 pobj outcome,of
R3124 T13162 T13161 amod good,outcome
R3125 T13163 T13155 punct ", ",been
R3126 T13164 T13155 expl there,been
R3127 T13165 T13155 aux have,been
R3128 T13166 T13167 amod inadequate,studies
R3129 T13167 T13155 attr studies,been
R313 T2661 T2660 prep of,types
R3130 T13168 T13169 npadvmod population,based
R3131 T13169 T13167 amod based,studies
R3132 T13170 T13169 punct -,based
R3133 T13171 T13172 dep that,include
R3134 T13172 T13167 relcl include,studies
R3135 T13173 T13174 amod fetal,cases
R3136 T13174 T13172 dobj cases,include
R3137 T13175 T13173 cc or,fetal
R3138 T13176 T13173 conj neonatal,fetal
R3139 T13177 T13174 cc and,cases
R314 T2662 T2661 pobj defects,of
R3140 T13178 T13179 compound autopsy,diagnoses
R3141 T13179 T13174 conj diagnoses,cases
R3142 T13180 T13181 aux to,make
R3143 T13181 T13172 advcl make,include
R3144 T13182 T13183 det this,conclusion
R3145 T13183 T13184 nsubj conclusion,definitive
R3146 T13184 T13181 ccomp definitive,make
R3147 T13185 T13155 punct .,been
R3148 T13187 T13188 prep In,indicate
R3149 T13189 T13187 pobj fact,In
R315 T2663 T2654 aux can,be
R3150 T13190 T13188 punct ", ",indicate
R3151 T13191 T13192 det the,series
R3152 T13192 T13188 nsubj series,indicate
R3153 T13193 T13192 prep of,series
R3154 T13194 T13193 pobj patients,of
R3155 T13195 T13196 nsubj we,report
R3156 T13196 T13194 advcl report,patients
R3157 T13197 T13196 advmod here,report
R3158 T13198 T13192 cc and,series
R3159 T13199 T13200 det the,literature
R316 T2664 T2654 acomp difficult,be
R3160 T13200 T13192 conj literature,series
R3161 T13201 T13200 amod published,literature
R3162 T13202 T13203 mark that,associated
R3163 T13203 T13188 ccomp associated,indicate
R3164 T13204 T13205 det an,defect
R3165 T13205 T13203 nsubjpass defect,associated
R3166 T13206 T13205 compound eventration,defect
R3167 T13207 T13203 aux may,associated
R3168 T13208 T13203 auxpass be,associated
R3169 T13209 T13203 prep with,associated
R317 T2665 T2654 prep without,be
R3170 T13210 T13209 pobj displacement,with
R3171 T13211 T13210 prep of,displacement
R3172 T13212 T13213 amod abdominal,contents
R3173 T13213 T13211 pobj contents,of
R3174 T13214 T13209 cc and,with
R3175 T13215 T13214 advmod also,and
R3176 T13216 T13209 conj with,with
R3177 T13217 T13218 amod severe,hypoplasia
R3178 T13218 T13216 pobj hypoplasia,with
R3179 T13219 T13218 amod pulmonary,hypoplasia
R318 T2666 T2667 amod direct,examination
R3180 T13220 T13218 cc and,hypoplasia
R3181 T13221 T13222 amod respiratory,insufficiency
R3182 T13222 T13218 conj insufficiency,hypoplasia
R3183 T13223 T13224 punct [,34
R3184 T13224 T13188 parataxis 34,indicate
R3185 T13225 T13224 nummod 33,34
R3186 T13226 T13224 punct ",",34
R3187 T13227 T13224 punct ],34
R3188 T13228 T13188 punct .,indicate
R3189 T13230 T13231 amod Numerous,abnormalities
R319 T2667 T2665 pobj examination,without
R3190 T13231 T13233 nsubjpass abnormalities,found
R3191 T13232 T13231 compound chromosome,abnormalities
R3192 T13234 T13233 aux have,found
R3193 T13235 T13233 auxpass been,found
R3194 T13236 T13233 prep in,found
R3195 T13237 T13236 pobj association,in
R3196 T13238 T13237 prep with,association
R3197 T13239 T13240 amod congenital,abnormalities
R3198 T13240 T13238 pobj abnormalities,with
R3199 T13241 T13240 compound diaphragm,abnormalities
R320 T2668 T2667 amod gross,examination
R3200 T13242 T13243 punct [,35
R3201 T13243 T13233 parataxis 35,found
R3202 T13244 T13243 nummod 12,35
R3203 T13245 T13243 punct ",",35
R3204 T13246 T13243 punct ],35
R3205 T13247 T13233 punct .,found
R3206 T13249 T13250 amod Human,FOG2
R3207 T13250 T13251 nsubj FOG2,maps
R3208 T13252 T13251 prep to,maps
R3209 T13253 T13252 pobj Chromosome,to
R321 T2669 T2667 punct (,examination
R3210 T13254 T13253 nummod 8q23.1,Chromosome
R3211 T13255 T13251 punct ", ",maps
R3212 T13256 T13251 cc and,maps
R3213 T13257 T13251 punct ", ",maps
R3214 T13258 T13259 advmod importantly,reported
R3215 T13259 T13251 conj reported,maps
R3216 T13260 T13259 punct ", ",reported
R3217 T13261 T13262 amod several,patients
R3218 T13262 T13259 nsubjpass patients,reported
R3219 T13263 T13262 prep with,patients
R322 T2670 T2667 amod intraoperative,examination
R3220 T13264 T13265 compound diaphragm,defects
R3221 T13265 T13263 pobj defects,with
R3222 T13266 T13265 cc and,defects
R3223 T13267 T13265 conj rearrangements,defects
R3224 T13268 T13267 acl involving,rearrangements
R3225 T13269 T13270 det this,locus
R3226 T13270 T13268 dobj locus,involving
R3227 T13271 T13259 aux have,reported
R3228 T13272 T13259 auxpass been,reported
R3229 T13273 T13259 punct .,reported
R323 T2671 T2670 cc or,intraoperative
R3230 T13275 T13276 advmod Specifically,are
R3231 T13277 T13276 punct ", ",are
R3232 T13278 T13276 expl there,are
R3233 T13279 T13280 nummod three,patients
R3234 T13280 T13276 attr patients,are
R3235 T13281 T13280 amod unrelated,patients
R3236 T13282 T13280 compound CDH,patients
R3237 T13283 T13280 prep with,patients
R3238 T13284 T13285 advmod cytogenetically,balanced
R3239 T13285 T13286 amod balanced,translocations
R324 T2672 T2670 conj postmortem,intraoperative
R3240 T13286 T13283 pobj translocations,with
R3241 T13287 T13286 prep at,translocations
R3242 T13288 T13287 cc or,at
R3243 T13289 T13287 conj near,at
R3244 T13290 T13291 det the,locus
R3245 T13291 T13289 pobj locus,near
R3246 T13292 T13291 compound FOG2,locus
R3247 T13293 T13294 punct [,37
R3248 T13294 T13276 parataxis 37,are
R3249 T13295 T13294 nummod 36,37
R325 T2673 T2667 punct ),examination
R3250 T13296 T13294 punct ",",37
R3251 T13297 T13294 punct ],37
R3252 T13298 T13276 punct .,are
R3253 T13300 T13301 advmod Additionally,died
R3254 T13302 T13301 punct ", ",died
R3255 T13303 T13304 nummod two,patients
R3256 T13304 T13301 nsubj patients,died
R3257 T13305 T13304 prep with,patients
R3258 T13306 T13305 pobj deletions,with
R3259 T13307 T13308 advmod apparently,encompassing
R326 T2674 T2654 punct .,be
R3260 T13308 T13306 acl encompassing,deletions
R3261 T13309 T13310 det the,locus
R3262 T13310 T13308 dobj locus,encompassing
R3263 T13311 T13310 compound FOG2,locus
R3264 T13312 T13301 aux have,died
R3265 T13313 T13301 prep from,died
R3266 T13314 T13315 amod multiple,anomalies
R3267 T13315 T13313 pobj anomalies,from
R3268 T13316 T13315 amod congenital,anomalies
R3269 T13317 T13315 prep including,anomalies
R327 T2676 T2677 amod Pulmonary,hypoplasia
R3270 T13318 T13317 pobj CDH,including
R3271 T13319 T13320 punct [,38
R3272 T13320 T13301 parataxis 38,died
R3273 T13321 T13322 punct –,40
R3274 T13322 T13320 prep 40,38
R3275 T13323 T13320 punct ],38
R3276 T13324 T13301 punct .,died
R3277 T13326 T13327 nsubj Inactivation,result
R3278 T13328 T13326 prep of,Inactivation
R3279 T13329 T13330 det this,gene
R328 T2677 T2678 nsubj hypoplasia,causes
R3280 T13330 T13328 pobj gene,of
R3281 T13331 T13326 prep due,Inactivation
R3282 T13332 T13331 pcomp to,due
R3283 T13333 T13334 amod chromosomal,rearrangement
R3284 T13334 T13331 pobj rearrangement,due
R3285 T13335 T13334 cc or,rearrangement
R3286 T13336 T13334 conj deletion,rearrangement
R3287 T13337 T13327 aux would,result
R3288 T13338 T13327 prep in,result
R3289 T13339 T13340 det a,mutation
R329 T2679 T2677 amod associated,hypoplasia
R3290 T13340 T13338 pobj mutation,in
R3291 T13341 T13340 amod heterozygous,mutation
R3292 T13342 T13340 amod null,mutation
R3293 T13343 T13340 amod similar,mutation
R3294 T13344 T13343 prep to,similar
R3295 T13345 T13344 pobj that,to
R3296 T13346 T13345 acl found,that
R3297 T13347 T13346 prep in,found
R3298 T13348 T13349 det the,patient
R3299 T13349 T13347 pobj patient,in
R330 T2680 T2679 prep with,associated
R3300 T13350 T13351 nsubj we,report
R3301 T13351 T13349 advcl report,patient
R3302 T13352 T13327 punct .,result
R3303 T13354 T13355 mark Because,is
R3304 T13355 T13361 advcl is,suggest
R3305 T13356 T13357 det the,mutation
R3306 T13463 T13464 advmod more,common
R3307 T13464 T13462 amod common,CDH
R3308 T13357 T13355 nsubj mutation,is
R3309 T13465 T13462 compound Bochdalek,CDH
R331 T2681 T2682 det these,defects
R3310 T13358 T13357 compound FOG2,mutation
R3311 T13359 T13360 nsubj we,report
R3312 T13466 T13452 punct ", ",is
R3313 T13360 T13357 advcl report,mutation
R3314 T13467 T13468 mark as,are
R3315 T13362 T13363 advmod de,novo
R3316 T13363 T13355 acomp novo,is
R3317 T13468 T13452 advcl are,is
R3318 T13364 T13355 cc and,is
R3319 T13365 T13366 det the,phenotypes
R332 T2682 T2680 pobj defects,with
R3320 T13469 T13470 det the,mechanisms
R3321 T13366 T13367 nsubj phenotypes,are
R3322 T13367 T13355 conj are,is
R3323 T13368 T13366 prep of,phenotypes
R3324 T13470 T13468 nsubj mechanisms,are
R3325 T13369 T13370 det the,defects
R3326 T13370 T13368 pobj defects,of
R3327 T13371 T13370 amod pulmonary,defects
R3328 T13471 T13470 amod pathogenic,mechanisms
R3329 T13372 T13371 cc and,pulmonary
R333 T2683 T2682 amod diaphragmatic,defects
R3330 T13373 T13371 conj diaphragmatic,pulmonary
R3331 T13374 T13367 acomp similar,are
R3332 T13472 T13470 prep for,mechanisms
R3333 T13473 T13472 pobj both,for
R3334 T13375 T13367 prep between,are
R3335 T13474 T13475 advmod largely,unknown
R3336 T13376 T13375 pobj mouse,between
R3337 T13377 T13376 cc and,mouse
R3338 T13475 T13468 acomp unknown,are
R3339 T13378 T13376 conj human,mouse
R334 T2684 T2685 amod severe,mortality
R3340 T13379 T13361 punct ", ",suggest
R3341 T13476 T13452 punct .,is
R3342 T13380 T13361 nsubj we,suggest
R3343 T13381 T13382 mark that,is
R3344 T13478 T13479 compound Muscle,precursors
R3345 T13382 T13361 ccomp is,suggest
R3346 T13383 T13384 det this,mutation
R3347 T13384 T13382 nsubj mutation,is
R3348 T13385 T13384 prep in,mutation
R3349 T13479 T13480 nsubj precursors,migrate
R335 T2685 T2678 dobj mortality,causes
R3350 T13386 T13385 pobj FOG2,in
R3351 T13387 T13388 det the,cause
R3352 T13481 T13479 acl destined,precursors
R3353 T13388 T13382 attr cause,is
R3354 T13389 T13388 amod first,cause
R3355 T13390 T13388 amod reported,cause
R3356 T13391 T13388 prep of,cause
R3357 T13482 T13483 aux to,populate
R3358 T13392 T13393 det a,defect
R3359 T13393 T13391 pobj defect,of
R336 T2686 T2685 cc and,mortality
R3360 T13394 T13393 amod human,defect
R3361 T13395 T13393 amod developmental,defect
R3362 T13396 T13393 amod diaphragmatic,defect
R3363 T13483 T13481 xcomp populate,destined
R3364 T13397 T13396 cc and,diaphragmatic
R3365 T13398 T13396 conj pulmonary,diaphragmatic
R3366 T13399 T13361 punct .,suggest
R3367 T13484 T13485 det the,diaphragm
R3368 T13401 T13402 prep In,appear
R3369 T13485 T13483 dobj diaphragm,populate
R337 T2687 T2685 conj morbidity,mortality
R3370 T13403 T13401 pobj contrast,In
R3371 T13404 T13403 prep to,contrast
R3372 T13486 T13480 prep from,migrate
R3373 T13405 T13406 det the,child
R3374 T13406 T13404 pobj child,to
R3375 T13407 T13406 amod affected,child
R3376 T13487 T13488 det the,dermomyotome
R3377 T13408 T13402 punct ", ",appear
R3378 T13409 T13402 nsubj mice,appear
R3379 T13488 T13486 pobj dermomyotome,from
R338 T2688 T2678 punct .,causes
R3380 T13410 T13409 amod heterozygous,mice
R3381 T13411 T13410 prep for,heterozygous
R3382 T13412 T13413 det a,mutation
R3383 T13489 T13488 amod lateral,dermomyotome
R3384 T13413 T13411 pobj mutation,for
R3385 T13414 T13413 amod null,mutation
R3386 T13415 T13413 prep of,mutation
R3387 T13490 T13488 prep of,dermomyotome
R3388 T13416 T13415 pobj Fog2,of
R3389 T13417 T13402 oprd normal,appear
R339 T2690 T2691 det The,pathogenesis
R3390 T13491 T13492 amod cervical,somites
R3391 T13418 T13402 punct .,appear
R3392 T13420 T13421 advmod However,is
R3393 T13492 T13490 pobj somites,of
R3394 T13422 T13421 punct ", ",is
R3395 T13493 T13480 punct .,migrate
R3396 T13423 T13421 expl there,is
R3397 T13424 T13425 amod ample,precedent
R3398 T13425 T13421 attr precedent,is
R3399 T13495 T13496 advmod Prior,to
R340 T2691 T2692 nsubjpass pathogenesis,understood
R3400 T13426 T13425 prep for,precedent
R3401 T13427 T13428 det the,observation
R3402 T13428 T13426 pobj observation,for
R3403 T13429 T13430 mark that,is
R3404 T13496 T13497 prep to,populate
R3405 T13430 T13428 acl is,observation
R3406 T13498 T13496 pobj migration,to
R3407 T13431 T13430 nsubj haploinsufficiency,is
R3408 T13432 T13431 prep of,haploinsufficiency
R3409 T13433 T13434 det a,gene
R341 T2693 T2691 cc and,pathogenesis
R3410 T13434 T13432 pobj gene,of
R3411 T13435 T13434 prep with,gene
R3412 T13436 T13437 amod developmental,functions
R3413 T13437 T13435 pobj functions,with
R3414 T13499 T13498 prep onto,migration
R3415 T13438 T13439 advmod much,less
R3416 T13439 T13440 advmod less,well
R3417 T13440 T13441 advmod well,tolerated
R3418 T13441 T13430 acomp tolerated,is
R3419 T13500 T13501 det the,diaphragm
R342 T2694 T2695 amod developmental,relationship
R3420 T13442 T13430 prep in,is
R3421 T13443 T13442 pobj humans,in
R3422 T13501 T13499 pobj diaphragm,onto
R3423 T13444 T13430 prep than,is
R3424 T13445 T13444 pobj mice,than
R3425 T13446 T13447 punct [,41
R3426 T13502 T13497 punct ", ",populate
R3427 T13447 T13421 parataxis 41,is
R3428 T13448 T13447 punct ],41
R3429 T13503 T13497 nsubj they,populate
R343 T2695 T2691 conj relationship,pathogenesis
R3430 T13449 T13421 punct .,is
R3431 T13451 T13452 nsubj It,is
R3432 T13504 T13505 det the,PPF
R3433 T13453 T13452 acomp unclear,is
R3434 T13454 T13455 advmod how,relates
R3435 T13505 T13497 dobj PPF,populate
R3436 T13455 T13452 ccomp relates,is
R3437 T13456 T13457 det the,defect
R3438 T13506 T13505 punct ", ",PPF
R3439 T13457 T13455 nsubj defect,relates
R344 T2696 T2695 prep between,relationship
R3440 T13458 T13457 nmod Fog2,defect
R3441 T13459 T13457 amod diaphragmatic,defect
R3442 T13507 T13508 det a,tissue
R3443 T13460 T13455 prep to,relates
R3444 T13461 T13462 det the,CDH
R3445 T13508 T13505 appos tissue,PPF
R3446 T13509 T13510 npadvmod wedge,shaped
R3447 T13462 T13460 pobj CDH,to
R3448 T13510 T13508 amod shaped,tissue
R3449 T13511 T13510 punct -,shaped
R345 T2697 T2698 amod diaphragmatic,defects
R3450 T13512 T13513 dep that,tapers
R3451 T13513 T13508 relcl tapers,tissue
R3452 T13514 T13513 advmod medially,tapers
R3453 T13515 T13513 prep from,tapers
R3454 T13570 T13571 advmod Thus,results
R3455 T13516 T13517 det the,wall
R3456 T13572 T13571 punct ", ",results
R3457 T13517 T13515 pobj wall,from
R3458 T13573 T13574 det a,defect
R3459 T13574 T13571 nsubj defect,results
R346 T2698 T2696 pobj defects,between
R3460 T13518 T13517 amod lateral,wall
R3461 T13575 T13574 prep in,defect
R3462 T13576 T13577 compound PPF,formation
R3463 T13577 T13575 pobj formation,in
R3464 T13578 T13571 advmod subsequently,results
R3465 T13579 T13571 prep in,results
R3466 T13580 T13581 det the,formation
R3467 T13519 T13517 compound body,wall
R3468 T13581 T13579 pobj formation,in
R3469 T13582 T13581 amod abnormal,formation
R347 T2699 T2698 cc and,defects
R3470 T13583 T13581 prep of,formation
R3471 T13520 T13513 prep to,tapers
R3472 T13584 T13585 det the,diaphragm
R3473 T13585 T13583 pobj diaphragm,of
R3474 T13521 T13522 det the,mesentery
R3475 T13586 T13587 punct [,43
R3476 T13587 T13571 parataxis 43,results
R3477 T13588 T13587 punct ],43
R3478 T13589 T13571 punct .,results
R3479 T13522 T13520 pobj mesentery,to
R348 T2700 T2701 amod pulmonary,hypoplasia
R3480 T13591 T13592 nsubj We,shown
R3481 T13523 T13522 amod esophageal,mesentery
R3482 T13593 T13592 aux have,shown
R3483 T13594 T13595 mark that,have
R3484 T13524 T13513 cc and,tapers
R3485 T13595 T13592 ccomp have,shown
R3486 T13596 T13597 det the,mutant
R3487 T13597 T13595 nsubj mutant,have
R3488 T13525 T13513 conj fuses,tapers
R3489 T13598 T13597 compound Fog2,mutant
R349 T2701 T2698 conj hypoplasia,defects
R3490 T13599 T13595 aux does,have
R3491 T13600 T13601 det an,pattern
R3492 T13526 T13525 advmod ventrally,fuses
R3493 T13601 T13595 dobj pattern,have
R3494 T13602 T13601 amod abnormal,pattern
R3495 T13527 T13525 prep with,fuses
R3496 T13603 T13601 prep of,pattern
R3497 T13604 T13605 compound HGF,expression
R3498 T13605 T13603 pobj expression,of
R3499 T13528 T13529 det the,transversum
R350 T2702 T2692 auxpass is,understood
R3500 T13606 T13595 prep in,have
R3501 T13607 T13608 det the,region
R3502 T13608 T13606 pobj region,in
R3503 T13529 T13527 pobj transversum,with
R3504 T13609 T13610 prep through,migrate
R3505 T13610 T13608 relcl migrate,region
R3506 T13611 T13609 pobj which,through
R3507 T13530 T13529 compound septum,transversum
R3508 T13612 T13613 compound muscle,cells
R3509 T13613 T13610 nsubj cells,migrate
R351 T2703 T2692 neg not,understood
R3510 T13531 T13532 punct [,42
R3511 T13614 T13613 compound precursor,cells
R3512 T13615 T13610 prep onto,migrate
R3513 T13616 T13617 det the,diaphragm
R3514 T13617 T13615 pobj diaphragm,onto
R3515 T13618 T13617 amod developing,diaphragm
R3516 T13619 T13592 punct .,shown
R3517 T13532 T13497 parataxis 42,populate
R3518 T13533 T13532 punct ],42
R3519 T13621 T13622 det This,finding
R352 T2704 T2692 punct .,understood
R3520 T13534 T13497 punct .,populate
R3521 T13622 T13623 nsubj finding,account
R3522 T13536 T13537 compound Muscle,precursors
R3523 T13624 T13623 aux may,account
R3524 T13625 T13623 prep for,account
R3525 T13626 T13627 det the,muscle
R3526 T13537 T13538 nsubj precursors,reach
R3527 T13627 T13625 pobj muscle,for
R3528 T13628 T13629 advmod abnormally,patterned
R3529 T13629 T13627 amod patterned,muscle
R353 T2706 T2707 mark Although,decreased
R3530 T13539 T13540 det the,PPF
R3531 T13630 T13631 dep that,develops
R3532 T13631 T13627 relcl develops,muscle
R3533 T13632 T13631 prep in,develops
R3534 T13633 T13634 det the,diaphragm
R3535 T13540 T13538 dobj PPF,reach
R3536 T13541 T13538 prep by,reach
R3537 T13634 T13632 pobj diaphragm,in
R3538 T13542 T13541 pobj E11,by
R3539 T13635 T13636 compound Fog2,mutant
R354 T2707 T2718 advcl decreased,reported
R3540 T13636 T13634 compound mutant,diaphragm
R3541 T13637 T13623 punct .,account
R3542 T13543 T13538 punct ", ",reach
R3543 T13639 T13640 mark Although,detected
R3544 T13544 T13545 advmod where,proliferate
R3545 T13640 T13646 advcl detected,need
R3546 T13641 T13642 nmod Pax3,expression
R3547 T13642 T13640 nsubjpass expression,detected
R3548 T13545 T13538 ccomp proliferate,reach
R3549 T13643 T13641 cc and,Pax3
R355 T2708 T2707 nsubj advances,decreased
R3550 T13546 T13545 nsubj they,proliferate
R3551 T13644 T13641 conj MyoD,Pax3
R3552 T13547 T13545 punct ", ",proliferate
R3553 T13645 T13640 auxpass is,detected
R3554 T13548 T13545 conj differentiate,proliferate
R3555 T13647 T13640 prep in,detected
R3556 T13648 T13649 det the,PPF
R3557 T13649 T13647 pobj PPF,in
R3558 T13549 T13548 punct ", ",differentiate
R3559 T13650 T13646 punct ", ",need
R356 T2709 T2708 prep in,advances
R3560 T13651 T13652 det a,analysis
R3561 T13652 T13646 nsubj analysis,need
R3562 T13550 T13548 cc and,differentiate
R3563 T13653 T13652 amod detailed,analysis
R3564 T13654 T13652 prep of,analysis
R3565 T13655 T13656 compound transcription,factors
R3566 T13656 T13654 pobj factors,of
R3567 T13657 T13656 amod responsible,factors
R3568 T13658 T13657 prep for,responsible
R3569 T13551 T13552 advmod then,migrate
R357 T2710 T2711 det the,management
R3570 T13659 T13660 compound muscle,cell
R3571 T13660 T13658 pobj cell,for
R3572 T13661 T13660 compound precursor,cell
R3573 T13662 T13660 appos migration,cell
R3574 T13552 T13548 conj migrate,differentiate
R3575 T13663 T13662 cc and,migration
R3576 T13664 T13662 conj differentiation,migration
R3577 T13553 T13552 prep toward,migrate
R3578 T13665 T13646 aux will,need
R3579 T13666 T13667 aux to,completed
R358 T2711 T2709 pobj management,in
R3580 T13667 T13646 xcomp completed,need
R3581 T13554 T13555 det the,regions
R3582 T13668 T13667 auxpass be,completed
R3583 T13669 T13670 preconj both,in
R3584 T13670 T13667 prep in,completed
R3585 T13555 T13553 pobj regions,toward
R3586 T13671 T13672 det the,PPF
R3587 T13672 T13670 pobj PPF,in
R3588 T13556 T13557 amod dorsolateral,costal
R3589 T13673 T13670 cc and,in
R359 T2712 T2711 amod medical,management
R3590 T13674 T13670 conj along,in
R3591 T13557 T13555 amod costal,regions
R3592 T13558 T13557 punct ", ",costal
R3593 T13559 T13560 amod sternal,costal
R3594 T13560 T13557 conj costal,costal
R3595 T13561 T13560 punct ", ",costal
R3596 T13675 T13676 det the,pathway
R3597 T13676 T13674 pobj pathway,along
R3598 T13677 T13676 prep of,pathway
R3599 T13562 T13560 cc and,costal
R360 T2713 T2711 prep of,management
R3600 T13678 T13679 compound muscle,cell
R3601 T13679 T13681 compound cell,migration
R3602 T13680 T13679 compound precursor,cell
R3603 T13563 T13560 conj crural,costal
R3604 T13681 T13677 pobj migration,of
R3605 T13682 T13676 prep between,pathway
R3606 T13564 T13555 prep of,regions
R3607 T13683 T13684 det the,PPF
R3608 T13684 T13682 pobj PPF,between
R3609 T13685 T13684 cc and,PPF
R361 T2714 T2715 amod pulmonary,hypoplasia
R3610 T13565 T13566 det the,diaphragm
R3611 T13686 T13687 det the,diaphragm
R3612 T13687 T13684 conj diaphragm,PPF
R3613 T13688 T13687 amod membranous,diaphragm
R3614 T13566 T13564 pobj diaphragm,of
R3615 T13689 T13646 punct .,need
R3616 T13691 T13692 nsubj Fog2,interact
R3617 T13567 T13566 amod developing,diaphragm
R3618 T13693 T13692 aux can,interact
R3619 T13694 T13692 prep with,interact
R362 T2715 T2713 pobj hypoplasia,of
R3620 T13695 T13694 pobj any,with
R3621 T13696 T13695 prep of,any
R3622 T13697 T13698 det the,factors
R3623 T13568 T13538 punct .,reach
R3624 T13698 T13696 pobj factors,of
R3625 T13699 T13698 compound Gata,factors
R3626 T13700 T13695 punct ", ",any
R3627 T13701 T13702 nmod Gatas,1
R3628 T13781 T13780 prep with,associated
R3629 T13702 T13695 appos 1,any
R363 T2716 T2707 aux may,decreased
R3630 T13703 T13704 punct –,6
R3631 T13704 T13702 prep 6,1
R3632 T13705 T13695 punct ", ",any
R3633 T13782 T13783 amod abnormal,anatomy
R3634 T13706 T13707 advmod as,as
R3635 T13707 T13695 cc as,any
R3636 T13708 T13707 advmod well,as
R3637 T13783 T13781 pobj anatomy,with
R3638 T13709 T13710 amod other,factors
R3639 T13710 T13695 conj factors,any
R364 T2717 T2707 aux have,decreased
R3640 T13711 T13710 compound transcription,factors
R3641 T13784 T13783 amod diaphragmatic,anatomy
R3642 T13712 T13713 amod such,as
R3643 T13713 T13710 prep as,factors
R3644 T13714 T13713 pobj CoupTFII,as
R3645 T13785 T13783 cc or,anatomy
R3646 T13715 T13716 punct [,45
R3647 T13716 T13692 parataxis 45,interact
R3648 T13717 T13716 nummod 44,45
R3649 T13786 T13783 conj function,anatomy
R365 T2719 T2720 det the,mortality
R3650 T13718 T13716 punct ",",45
R3651 T13719 T13716 punct ],45
R3652 T13787 T13779 punct ", ",is
R3653 T13720 T13692 punct .,interact
R3654 T13788 T13779 cc and,is
R3655 T13722 T13723 nsubjpass It,known
R3656 T13789 T13790 auxpass is,known
R3657 T13724 T13723 auxpass is,known
R3658 T13790 T13779 conj known,is
R3659 T13725 T13726 mark that,is
R366 T2720 T2707 dobj mortality,decreased
R3660 T13726 T13723 ccomp is,known
R3661 T13727 T13728 det a,interaction
R3662 T13791 T13792 aux to,occur
R3663 T13728 T13726 nsubj interaction,is
R3664 T13729 T13730 compound Fog2,Gata4
R3665 T13792 T13790 xcomp occur,known
R3666 T13730 T13728 compound Gata4,interaction
R3667 T13731 T13730 punct –,Gata4
R3668 T13732 T13726 acomp critical,is
R3669 T13793 T13792 prep as,occur
R367 T2721 T2720 amod associated,mortality
R3670 T13733 T13732 prep for,critical
R3671 T13734 T13735 amod normal,development
R3672 T13735 T13733 pobj development,for
R3673 T13736 T13735 amod cardiac,development
R3674 T13737 T13736 cc and,cardiac
R3675 T13738 T13736 conj gonadal,cardiac
R3676 T13794 T13795 det a,defect
R3677 T13739 T13723 punct ", ",known
R3678 T13740 T13723 cc but,known
R3679 T13741 T13742 amod interacting,factors
R368 T2722 T2721 prep with,associated
R3680 T13795 T13793 pobj defect,as
R3681 T13742 T13743 nsubjpass factors,determined
R3682 T13743 T13723 conj determined,known
R3683 T13744 T13742 prep in,factors
R3684 T13796 T13795 amod secondary,defect
R3685 T13745 T13746 det the,lung
R3686 T13746 T13744 pobj lung,in
R3687 T13747 T13746 cc and,lung
R3688 T13797 T13795 amod developmental,defect
R3689 T13748 T13746 conj diaphragm,lung
R369 T2723 T2724 compound CDH,patients
R3690 T13749 T13743 aux have,determined
R3691 T13750 T13743 neg not,determined
R3692 T13798 T13792 prep in,occur
R3693 T13751 T13743 advmod yet,determined
R3694 T13752 T13743 auxpass been,determined
R3695 T13799 T13798 pobj models,in
R3696 T13753 T13743 punct .,determined
R3697 T13755 T13756 det The,severity
R3698 T13800 T13799 prep of,models
R3699 T13756 T13757 nsubj severity,was
R370 T2724 T2722 pobj patients,with
R3700 T13801 T13802 amod diaphragmatic,dysfunction
R3701 T13758 T13756 prep of,severity
R3702 T13759 T13760 amod pulmonary,hypoplasia
R3703 T13760 T13758 pobj hypoplasia,of
R3704 T13761 T13756 prep in,severity
R3705 T13802 T13800 pobj dysfunction,of
R3706 T13762 T13763 det the,patient
R3707 T13763 T13761 pobj patient,in
R3708 T13803 T13804 amod such,as
R3709 T13764 T13765 nsubj we,report
R371 T2725 T2726 dep who,survive
R3710 T13765 T13763 advcl report,patient
R3711 T13766 T13757 prep out,was
R3712 T13767 T13766 prep of,out
R3713 T13804 T13802 prep as,dysfunction
R3714 T13768 T13767 pobj proportion,of
R3715 T13769 T13768 prep to,proportion
R3716 T13805 T13806 amod complete,amuscularization
R3717 T13770 T13769 pobj that,to
R3718 T13771 T13770 prep of,that
R3719 T13772 T13773 det the,defect
R372 T2726 T2724 relcl survive,patients
R3720 T13806 T13804 pobj amuscularization,as
R3721 T13773 T13771 pobj defect,of
R3722 T13774 T13773 compound diaphragm,defect
R3723 T13775 T13757 punct .,was
R3724 T13777 T13778 amod Pulmonary,hypoplasia
R3725 T13807 T13808 punct [,17
R3726 T13778 T13779 nsubj hypoplasia,is
R3727 T13780 T13779 acomp associated,is
R3728 T13808 T13806 parataxis 17,amuscularization
R3729 T13809 T13808 punct ],17
R373 T2727 T2728 aux to,receive
R3730 T13810 T13806 cc or,amuscularization
R3731 T13887 T13884 agent by,suspected
R3732 T13888 T13887 pobj clinicians,by
R3733 T13811 T13812 amod phrenic,nerve
R3734 T13889 T13890 dep who,care
R3735 T13890 T13888 relcl care,clinicians
R3736 T13891 T13890 prep for,care
R3737 T13812 T13813 compound nerve,disruption
R3738 T13892 T13893 det these,patients
R3739 T13813 T13806 conj disruption,amuscularization
R374 T2728 T2726 advcl receive,survive
R3740 T13814 T13815 punct [,46
R3741 T13815 T13813 parataxis 46,disruption
R3742 T13893 T13891 pobj patients,for
R3743 T13894 T13847 punct .,suggested
R3744 T13816 T13815 punct ],46
R3745 T13896 T13897 prep In,supports
R3746 T13817 T13779 punct .,is
R3747 T13898 T13896 pobj addition,In
R3748 T13899 T13897 punct ", ",supports
R3749 T13900 T13901 det the,incidence
R375 T2729 T2728 dobj care,receive
R3750 T13819 T13820 nsubj It,occurs
R3751 T13901 T13897 nsubj incidence,supports
R3752 T13902 T13901 amod high,incidence
R3753 T13903 T13901 prep of,incidence
R3754 T13821 T13820 prep in,occurs
R3755 T13904 T13905 compound lobar,abnormalities
R3756 T13905 T13903 pobj abnormalities,of
R3757 T13906 T13905 amod associated,abnormalities
R3758 T13907 T13906 prep with,associated
R3759 T13822 T13823 det a,model
R376 T2730 T2728 prep at,receive
R3760 T13908 T13907 pobj CDH,with
R3761 T13909 T13910 punct [,52
R3762 T13910 T13901 parataxis 52,incidence
R3763 T13823 T13821 pobj model,in
R3764 T13911 T13910 punct ],52
R3765 T13912 T13913 det the,possibility
R3766 T13913 T13897 dobj possibility,supports
R3767 T13824 T13823 amod surgical,model
R3768 T13914 T13915 mark that,associated
R3769 T13915 T13913 acl associated,possibility
R377 T2731 T2732 amod high,volume
R3770 T13825 T13823 prep of,model
R3771 T13916 T13917 det this,disorder
R3772 T13917 T13915 nsubjpass disorder,associated
R3773 T13918 T13915 aux can,associated
R3774 T13919 T13915 auxpass be,associated
R3775 T13920 T13915 prep with,associated
R3776 T13921 T13922 det a,abnormality
R3777 T13826 T13825 pobj CDH,of
R3778 T13922 T13920 pobj abnormality,with
R3779 T13923 T13922 amod primary,abnormality
R378 T2732 T2734 compound volume,centers
R3780 T13924 T13922 amod developmental,abnormality
R3781 T13827 T13828 prep in,created
R3782 T13925 T13922 amod pulmonary,abnormality
R3783 T13926 T13897 punct .,supports
R3784 T13828 T13823 relcl created,model
R3785 T13928 T13929 poss Our,analysis
R3786 T13929 T13930 nsubj analysis,proves
R3787 T13829 T13827 pobj which,in
R3788 T13931 T13929 prep of,analysis
R3789 T13932 T13931 pobj mice,of
R379 T2733 T2732 punct -,volume
R3790 T13830 T13831 det a,hernia
R3791 T13933 T13932 acl carrying,mice
R3792 T13934 T13933 dobj mutations,carrying
R3793 T13935 T13934 prep of,mutations
R3794 T13831 T13828 nsubjpass hernia,created
R3795 T13936 T13935 pobj Fog2,of
R3796 T13937 T13938 mark that,is
R3797 T13938 T13930 ccomp is,proves
R3798 T13832 T13828 auxpass is,created
R3799 T13939 T13938 expl there,is
R380 T2734 T2730 pobj centers,at
R3800 T13940 T13941 det a,defect
R3801 T13833 T13828 advmod physically,created
R3802 T13941 T13938 attr defect,is
R3803 T13942 T13941 amod primary,defect
R3804 T13943 T13941 prep in,defect
R3805 T13944 T13945 compound lung,development
R3806 T13834 T13828 prep in,created
R3807 T13945 T13943 pobj development,in
R3808 T13946 T13947 dep that,results
R3809 T13947 T13941 relcl results,defect
R381 T2735 T2736 punct [,3
R3810 T13835 T13836 det an,lamb
R3811 T13948 T13947 prep in,results
R3812 T13949 T13950 amod specific,loss
R3813 T13950 T13948 pobj loss,in
R3814 T13836 T13834 pobj lamb,in
R3815 T13951 T13950 prep of,loss
R3816 T13952 T13953 det the,lobe
R3817 T13953 T13951 pobj lobe,of
R3818 T13837 T13838 advmod in,utero
R3819 T13954 T13953 amod accessory,lobe
R382 T2736 T2707 parataxis 3,decreased
R3820 T13955 T13950 cc and,loss
R3821 T13956 T13957 amod partial,loss
R3822 T13838 T13836 amod utero,lamb
R3823 T13957 T13950 conj loss,loss
R3824 T13958 T13957 prep of,loss
R3825 T13959 T13960 det the,lobe
R3826 T13960 T13958 pobj lobe,of
R3827 T13961 T13960 amod right,lobe
R3828 T13839 T13840 punct [,48
R3829 T13962 T13960 amod middle,lobe
R383 T2737 T2736 nummod 2,3
R3830 T13963 T13930 punct .,proves
R3831 T13840 T13820 parataxis 48,occurs
R3832 T13965 T13966 det The,defects
R3833 T13966 T13969 nsubj defects,prompted
R3834 T13967 T13966 amod specific,defects
R3835 T13841 T13840 nummod 47,48
R3836 T13968 T13966 compound lobar,defects
R3837 T13970 T13969 dobj us,prompted
R3838 T13971 T13972 aux to,evaluate
R3839 T13842 T13840 punct ",",48
R384 T2738 T2736 punct ",",3
R3840 T13972 T13969 xcomp evaluate,prompted
R3841 T13973 T13974 compound Fog2,expression
R3842 T13843 T13840 punct ],48
R3843 T13974 T13972 dobj expression,evaluate
R3844 T13975 T13972 prep at,evaluate
R3845 T13976 T13977 det the,time
R3846 T13844 T13820 punct .,occurs
R3847 T13977 T13975 pobj time,at
R3848 T13978 T13977 prep of,time
R3849 T13979 T13980 amod early,budding
R385 T2739 T2736 punct ],3
R3850 T13846 T13847 advmod However,suggested
R3851 T13980 T13978 pobj budding,of
R3852 T13848 T13847 punct ", ",suggested
R3853 T13981 T13980 compound lobar,budding
R3854 T13982 T13969 punct .,prompted
R3855 T13849 T13850 det the,possibility
R3856 T13984 T13985 mark While,is
R3857 T13985 T13988 advcl is,expressed
R3858 T13986 T13987 compound Fog2,expression
R3859 T13850 T13847 nsubjpass possibility,suggested
R386 T2740 T2718 punct ", ",reported
R3860 T13987 T13985 nsubj expression,is
R3861 T13989 T13985 acomp diffuse,is
R3862 T13851 T13852 mark that,occur
R3863 T13990 T13985 prep in,is
R3864 T13991 T13992 det the,mesenchyme
R3865 T13992 T13990 pobj mesenchyme,in
R3866 T13852 T13850 acl occur,possibility
R3867 T13853 T13854 amod primary,abnormalities
R3868 T13854 T13852 nsubj abnormalities,occur
R3869 T13993 T13992 amod pulmonary,mesenchyme
R387 T2741 T2742 det the,mortality
R3870 T13994 T13995 mark after,is
R3871 T13855 T13854 amod pulmonary,abnormalities
R3872 T13995 T13985 advcl is,is
R3873 T13996 T13997 compound lobar,structure
R3874 T13856 T13854 amod developmental,abnormalities
R3875 T13997 T13995 nsubj structure,is
R3876 T13998 T13999 advmod well,established
R3877 T13999 T13995 acomp established,is
R3878 T14000 T14001 punct (,E12.5
R3879 T14001 T13995 parataxis E12.5,is
R388 T2742 T2718 nsubjpass mortality,reported
R3880 T14002 T14001 punct ),E12.5
R3881 T13857 T13852 prep with,occur
R3882 T14003 T13988 punct ", ",expressed
R3883 T14004 T13988 nsubjpass it,expressed
R3884 T14005 T13988 auxpass is,expressed
R3885 T13858 T13857 punct ", ",with
R3886 T14006 T14007 advmod more,focally
R3887 T14007 T13988 advmod focally,expressed
R3888 T14008 T13988 prep in,expressed
R3889 T13859 T13860 advmod rather,than
R389 T2743 T2744 npadvmod population,based
R3890 T14009 T14010 det the,mesenchyme
R3891 T13860 T13857 cc than,with
R3892 T13861 T13857 advmod secondary,with
R3893 T14010 T14008 pobj mesenchyme,in
R3894 T14011 T14010 acl surrounding,mesenchyme
R3895 T13862 T13861 prep to,secondary
R3896 T14012 T14013 det the,lobe
R3897 T14013 T14011 dobj lobe,surrounding
R3898 T13863 T13857 punct ", ",with
R3899 T14014 T14013 amod right,lobe
R390 T2744 T2742 amod based,mortality
R3900 T14015 T14013 amod middle,lobe
R3901 T14016 T14013 cc and,lobe
R3902 T13864 T13865 amod diaphragmatic,defects
R3903 T14017 T14018 amod accessory,buds
R3904 T14018 T14013 conj buds,lobe
R3905 T14019 T14020 mark as,form
R3906 T13865 T13857 conj defects,with
R3907 T14020 T13988 advcl form,expressed
R3908 T14021 T14022 det these,lobes
R3909 T13866 T13847 aux has,suggested
R391 T2745 T2744 punct -,based
R3910 T14022 T14020 nsubj lobes,form
R3911 T14023 T13988 punct .,expressed
R3912 T13867 T13847 auxpass been,suggested
R3913 T14025 T14026 nsubj This,matches
R3914 T13868 T13847 agent by,suggested
R3915 T14027 T14028 det the,phenotype
R3916 T14028 T14026 dobj phenotype,matches
R3917 T14029 T14028 prep of,phenotype
R3918 T13869 T13868 pobj others,by
R3919 T14030 T14031 amod right,lobe
R392 T2746 T2718 aux has,reported
R3920 T14031 T14033 nmod lobe,loss
R3921 T14032 T14031 amod middle,lobe
R3922 T13870 T13847 prep based,suggested
R3923 T14033 T14029 pobj loss,of
R3924 T14034 T14031 cc and,lobe
R3925 T14035 T14036 amod accessory,lobe
R3926 T13871 T13870 prep on,based
R3927 T14036 T14031 conj lobe,lobe
R3928 T14037 T14026 punct ", ",matches
R3929 T14038 T14026 cc and,matches
R393 T2747 T2718 auxpass been,reported
R3930 T14039 T14026 conj suggests,matches
R3931 T14040 T14041 mark that,has
R3932 T14041 T14039 ccomp has,suggests
R3933 T13872 T13873 det a,model
R3934 T14042 T14041 nsubj Fog2,has
R3935 T14043 T14044 det a,role
R3936 T14044 T14041 dobj role,has
R3937 T13873 T13871 pobj model,on
R3938 T14045 T14044 amod specific,role
R3939 T14046 T14044 compound patterning,role
R394 T2748 T2749 aux to,be
R3940 T14047 T14044 prep in,role
R3941 T13874 T13873 amod teratogenic,model
R3942 T14048 T14047 pobj establishment,in
R3943 T14049 T14048 prep of,establishment
R3944 T14050 T14051 det these,lobes
R3945 T13875 T13873 prep of,model
R3946 T14051 T14049 pobj lobes,of
R3947 T14052 T14026 punct .,matches
R3948 T13876 T13875 pobj CDH,of
R3949 T14054 T14055 nsubj It,is
R395 T2749 T2718 xcomp be,reported
R3950 T13877 T13878 punct [,49
R3951 T14056 T14057 advmod less,clear
R3952 T14057 T14055 acomp clear,is
R3953 T14058 T14059 mark whether,results
R3954 T13878 T13847 parataxis 49,suggested
R3955 T14059 T14055 ccomp results,is
R3956 T14060 T14059 nsubj loss,results
R3957 T14061 T14060 prep of,loss
R3958 T13879 T13880 punct –,51
R3959 T14062 T14061 pobj Fog2,of
R396 T2750 T2751 advmod as,62
R3960 T14063 T14059 prep in,results
R3961 T13880 T13878 prep 51,49
R3962 T14064 T14065 det a,defect
R3963 T13881 T13878 punct ],49
R3964 T14065 T14063 pobj defect,in
R3965 T13882 T13847 cc and,suggested
R3966 T14066 T14065 amod global,defect
R3967 T13883 T13884 aux has,suspected
R3968 T14067 T14065 compound branching,defect
R3969 T14068 T14055 punct ", ",is
R397 T2751 T2754 nummod 62,%
R3970 T14069 T14070 mark as,appear
R3971 T13884 T13847 conj suspected,suggested
R3972 T14070 T14055 advcl appear,is
R3973 T14071 T14072 compound Fog2,lungs
R3974 T14072 T14070 nsubj lungs,appear
R3975 T13885 T13884 advmod long,suspected
R3976 T14073 T14074 aux to,have
R3977 T14074 T14070 xcomp have,appear
R3978 T13886 T13884 auxpass been,suspected
R3979 T14075 T14076 det a,delay
R398 T2752 T2751 amod great,62
R3980 T14076 T14074 dobj delay,have
R3981 T14099 T14097 pobj lobes,in
R3982 T14077 T14076 amod slight,delay
R3983 T14078 T14076 amod developmental,delay
R3984 T14079 T14076 punct ", ",delay
R3985 T14080 T14081 dep which,result
R3986 T14081 T14076 relcl result,delay
R3987 T14082 T14081 aux could,result
R3988 T14083 T14081 prep from,result
R3989 T14100 T14099 amod unaffected,lobes
R399 T2753 T2751 quantmod as,62
R3990 T14084 T14085 amod many,causes
R3991 T14085 T14083 pobj causes,from
R3992 T14086 T14055 punct .,is
R3993 T14101 T14102 dep that,appears
R3994 T14088 T14089 amod Cultured,lungs
R3995 T14089 T14091 nsubj lungs,develop
R3996 T14090 T14089 compound Fog2,lungs
R3997 T14102 T14094 relcl appears,pattern
R3998 T14092 T14091 aux do,develop
R3999 T14093 T14094 det an,pattern
R400 T2754 T2749 attr %,be
R4000 T14103 T14102 oprd similar,appears
R4001 T14094 T14091 dobj pattern,develop
R4002 T14095 T14094 amod intricate,pattern
R4003 T14096 T14094 compound branching,pattern
R4004 T14104 T14103 prep in,similar
R4005 T14097 T14094 prep in,pattern
R4006 T14098 T14099 det the,lobes
R4007 T14105 T14106 det the,pattern
R4008 T14106 T14104 pobj pattern,in
R4009 T14107 T14103 prep to,similar
R401 T2755 T2718 punct ", ",reported
R4010 T14108 T14109 amod wild,type
R4011 T14206 T14207 nsubj It,is
R4012 T14109 T14111 compound type,lungs
R4013 T14208 T14209 advmod only,as
R4014 T14209 T14207 prep as,is
R4015 T14110 T14109 punct -,type
R4016 T14210 T14211 det a,consequence
R4017 T14211 T14209 pobj consequence,as
R4018 T14212 T14211 prep of,consequence
R4019 T14111 T14107 pobj lungs,to
R402 T2756 T2718 cc and,reported
R4020 T14213 T14214 npadvmod phenotype,driven
R4021 T14214 T14216 amod driven,analyses
R4022 T14215 T14214 punct -,driven
R4023 T14112 T14091 prep after,develop
R4024 T14216 T14212 pobj analyses,of
R4025 T14217 T14218 amod such,as
R4026 T14218 T14216 prep as,analyses
R4027 T14113 T14114 nummod 5,d
R4028 T14219 T14218 pobj those,as
R4029 T14220 T14221 nsubj we,pursuing
R403 T2757 T2758 expl there,are
R4030 T14221 T14219 advcl pursuing,those
R4031 T14114 T14112 pobj d,after
R4032 T14222 T14221 aux are,pursuing
R4033 T14223 T14224 mark that,has
R4034 T14224 T14207 ccomp has,is
R4035 T14225 T14224 nsubj one,has
R4036 T14226 T14227 det the,opportunity
R4037 T14115 T14114 prep in,d
R4038 T14227 T14224 dobj opportunity,has
R4039 T14228 T14229 aux to,assay
R404 T2758 T2718 conj are,reported
R4040 T14229 T14227 acl assay,opportunity
R4041 T14230 T14229 dobj all,assay
R4042 T14116 T14115 pobj culture,in
R4043 T14231 T14230 prep of,all
R4044 T14232 T14233 det the,derangements
R4045 T14233 T14231 pobj derangements,of
R4046 T14117 T14091 punct .,develop
R4047 T14234 T14233 amod potential,derangements
R4048 T14235 T14233 amod molecular,derangements
R4049 T14119 T14120 prep In,show
R405 T2759 T2760 det a,number
R4050 T14236 T14237 dep that,result
R4051 T14237 T14233 relcl result,derangements
R4052 T14238 T14237 aux may,result
R4053 T14121 T14122 det this,report
R4054 T14239 T14237 prep in,result
R4055 T14240 T14241 amod human,disease
R4056 T14122 T14119 pobj report,In
R4057 T14241 T14239 pobj disease,in
R4058 T14242 T14207 punct .,is
R4059 T14123 T14120 punct ", ",show
R406 T2760 T2758 attr number,are
R4060 T14124 T14120 nsubj we,show
R4061 T14125 T14126 mark that,causes
R4062 T14126 T14120 ccomp causes,show
R4063 T14127 T14128 det a,mutation
R4064 T14128 T14126 nsubj mutation,causes
R4065 T14129 T14128 prep of,mutation
R4066 T14130 T14129 pobj Fog2,of
R4067 T14131 T14128 prep in,mutation
R4068 T14132 T14133 det the,mouse
R4069 T14133 T14131 pobj mouse,in
R407 T2761 T2760 amod large,number
R4070 T14134 T14135 det the,phenotype
R4071 T14135 T14126 dobj phenotype,causes
R4072 T14136 T14135 prep of,phenotype
R4073 T14137 T14138 amod abnormal,muscularization
R4074 T14138 T14136 pobj muscularization,of
R4075 T14139 T14138 amod diaphragmatic,muscularization
R4076 T14140 T14138 cc and,muscularization
R4077 T14141 T14142 amod primary,hypoplasia
R4078 T14142 T14138 conj hypoplasia,muscularization
R4079 T14143 T14142 amod pulmonary,hypoplasia
R408 T2762 T2760 prep of,number
R4080 T14144 T14120 punct .,show
R4081 T14146 T14147 nsubj We,demonstrate
R4082 T14148 T14147 advmod furthermore,demonstrate
R4083 T14149 T14150 mark that,is
R4084 T14150 T14147 ccomp is,demonstrate
R4085 T14151 T14152 det a,mutation
R4086 T14152 T14150 nsubj mutation,is
R4087 T14153 T14152 prep in,mutation
R4088 T14154 T14155 det this,gene
R4089 T14155 T14153 pobj gene,in
R409 T2763 T2762 pobj deaths,of
R4090 T14156 T14150 acomp associated,is
R4091 T14157 T14156 prep with,associated
R4092 T14158 T14159 det a,defect
R4093 T14159 T14157 pobj defect,with
R4094 T14160 T14159 amod lethal,defect
R4095 T14161 T14159 prep in,defect
R4096 T14162 T14163 nmod lung,development
R4097 T14163 T14161 pobj development,in
R4098 T14164 T14162 cc and,lung
R4099 T14165 T14162 conj diaphragm,lung
R410 T2764 T2765 amod prior,to
R4100 T14166 T14159 prep in,defect
R4101 T14167 T14168 det a,child
R4102 T14168 T14166 pobj child,in
R4103 T14169 T14147 punct .,demonstrate
R4104 T14171 T14172 nsubj It,is
R4105 T14173 T14172 acomp notable,is
R4106 T14174 T14175 mark that,recognized
R4107 T14175 T14172 ccomp recognized,is
R4108 T14176 T14175 punct ", ",recognized
R4109 T14177 T14175 prep despite,recognized
R411 T2765 T2758 prep to,are
R4110 T14178 T14179 amod extensive,analysis
R4111 T14179 T14177 pobj analysis,despite
R4112 T14180 T14179 prep of,analysis
R4113 T14181 T14182 compound Fog2,biology
R4114 T14182 T14180 pobj biology,of
R4115 T14183 T14179 cc and,analysis
R4116 T14184 T14185 det the,generation
R4117 T14185 T14179 conj generation,analysis
R4118 T14186 T14185 prep of,generation
R4119 T14187 T14188 det a,mouse
R412 T2766 T2765 pobj birth,to
R4120 T14188 T14186 pobj mouse,of
R4121 T14189 T14188 nmod Fog2,mouse
R4122 T14190 T14188 amod knock,mouse
R4123 T14191 T14190 punct -,knock
R4124 T14192 T14190 prt out,knock
R4125 T14193 T14175 punct ", ",recognized
R4126 T14194 T14195 poss its,role
R4127 T14195 T14175 nsubjpass role,recognized
R4128 T14196 T14195 prep in,role
R4129 T14197 T14198 nmod diaphragm,development
R413 T2767 T2765 cc or,to
R4130 T14198 T14196 pobj development,in
R4131 T14199 T14197 cc and,diaphragm
R4132 T14200 T14197 conj lung,diaphragm
R4133 T14201 T14175 auxpass was,recognized
R4134 T14202 T14175 advmod previously,recognized
R4135 T14203 T14175 neg not,recognized
R4136 T14204 T14172 punct .,is
R4137 T14396 T14397 amod Genetic,mapping
R4138 T14398 T14397 prep of,mapping
R4139 T14399 T14400 det the,mutation
R414 T2768 T2765 conj to,to
R4140 T14400 T14398 pobj mutation,of
R4141 T14401 T14400 compound mouse,mutation
R4142 T14402 T14400 appos lil,mutation
R4143 T14403 T14397 punct .,mapping
R4144 T14405 T14406 det The,mutation
R4145 T14406 T14408 nsubjpass mutation,identified
R4146 T14407 T14406 compound lil,mutation
R4147 T14409 T14408 auxpass was,identified
R4148 T14410 T14411 mark as,described
R4149 T14411 T14408 advcl described,identified
R415 T2769 T2768 pobj transfer,to
R4150 T14412 T14411 prep in,described
R4151 T14413 T14412 pobj results,in
R4152 T14414 T14408 punct .,identified
R4153 T14416 T14417 amod Wild,type
R4154 T14417 T14419 nmod type,mice
R4155 T14418 T14417 punct -,type
R4156 T14419 T14427 nsubjpass mice,obtained
R4157 T14420 T14421 nmod FVB,N
R4158 T14421 T14419 nmod N,mice
R4159 T14422 T14421 punct /,N
R416 T2770 T2769 prep to,transfer
R4160 T14423 T14421 cc and,N
R4161 T14424 T14425 compound C57BL,6J
R4162 T14425 T14421 conj 6J,N
R4163 T14426 T14425 punct /,6J
R4164 T14428 T14419 acl used,mice
R4165 T14429 T14428 prep for,used
R4166 T14430 T14431 amod genetic,crosses
R4167 T14431 T14429 pobj crosses,for
R4168 T14432 T14427 auxpass were,obtained
R4169 T14433 T14427 prep from,obtained
R417 T2771 T2772 det a,facility
R4170 T14434 T14435 det the,Laboratory
R4171 T14435 T14433 pobj Laboratory,from
R4172 T14436 T14435 compound Jackson,Laboratory
R4173 T14437 T14438 punct (,Harbor
R4174 T14438 T14435 parataxis Harbor,Laboratory
R4175 T14439 T14438 compound Bar,Harbor
R4176 T14440 T14438 punct ", ",Harbor
R4177 T14441 T14438 npadvmod Maine,Harbor
R4178 T14442 T14438 punct ", ",Harbor
R4179 T14443 T14444 compound United,States
R418 T2772 T2770 pobj facility,to
R4180 T14444 T14438 npadvmod States,Harbor
R4181 T14445 T14438 punct ),Harbor
R4182 T14446 T14427 punct .,obtained
R4183 T14448 T14449 nsubj Mice,were
R4184 T14450 T14448 acl carrying,Mice
R4185 T14451 T14452 det a,mutation
R4186 T14452 T14450 dobj mutation,carrying
R4187 T14453 T14452 amod null,mutation
R4188 T14454 T14452 prep of,mutation
R4189 T14455 T14454 pobj Fog2,of
R419 T2773 T2774 amod tertiary,care
R4190 T14456 T14452 acl generated,mutation
R4191 T14457 T14456 prep by,generated
R4192 T14458 T14459 compound gene,targeting
R4193 T14459 T14457 pobj targeting,by
R4194 T14460 T14461 punct [,16
R4195 T14461 T14456 parataxis 16,generated
R4196 T14462 T14461 punct ],16
R4197 T14463 T14464 det the,gift
R4198 T14464 T14449 attr gift,were
R4199 T14465 T14464 amod generous,gift
R420 T2774 T2772 compound care,facility
R4200 T14466 T14464 prep of,gift
R4201 T14467 T14468 compound Dr.,Orkin
R4202 T14468 T14466 pobj Orkin,of
R4203 T14469 T14468 compound Stuart,Orkin
R4204 T14470 T14449 punct .,were
R4205 T15313 T15314 amod Developmental,analysis
R4206 T15315 T15314 prep of,analysis
R4207 T15316 T15315 pobj mice,of
R4208 T15317 T15314 punct .,analysis
R4209 T15319 T15320 amod Timed,pregnancies
R421 T2775 T2776 punct [,4
R4210 T15320 T15321 nsubjpass pregnancies,set
R4211 T15322 T15321 auxpass were,set
R4212 T15323 T15321 prt up,set
R4213 T15324 T15321 prep for,set
R4214 T15325 T15324 pobj collection,for
R4215 T15326 T15325 prep of,collection
R4216 T15327 T15328 nmod E11.5,embryos
R4217 T15328 T15326 pobj embryos,of
R4218 T15329 T15330 punct –,E17.5
R4219 T15330 T15327 prep E17.5,E11.5
R422 T2776 T2758 parataxis 4,are
R4220 T15331 T15321 punct .,set
R4221 T15333 T15334 nsubj Embryos,fixed
R4222 T15335 T15334 aux were,fixed
R4223 T15336 T15334 punct ", ",fixed
R4224 T15337 T15334 conj dehydrated,fixed
R4225 T15338 T15337 punct ", ",dehydrated
R4226 T15339 T15337 cc and,dehydrated
R4227 T15340 T15337 conj embedded,dehydrated
R4228 T15341 T15340 prep in,embedded
R4229 T15342 T15341 pobj paraffin,in
R423 T2777 T2776 punct ],4
R4230 T15343 T15344 amod prior,to
R4231 T15344 T15334 prep to,fixed
R4232 T15345 T15344 pobj sectioning,to
R4233 T15346 T15334 punct .,fixed
R4234 T15348 T15349 prep In,performed
R4235 T15350 T15351 amod older,embryos
R4236 T15351 T15348 pobj embryos,In
R4237 T15352 T15349 punct ", ",performed
R4238 T15353 T15354 det a,sternotomy
R4239 T15354 T15349 nsubjpass sternotomy,performed
R424 T2778 T2718 punct .,reported
R4240 T15355 T15354 amod median,sternotomy
R4241 T15356 T15349 auxpass was,performed
R4242 T15357 T15349 prep under,performed
R4243 T15358 T15359 amod microscopic,guidance
R4244 T15359 T15357 pobj guidance,under
R4245 T15360 T15349 punct ", ",performed
R4246 T15361 T15349 cc and,performed
R4247 T15362 T15363 nsubjpass diaphragm,examined
R4248 T15363 T15349 conj examined,performed
R4249 T15364 T15362 punct ", ",diaphragm
R425 T2780 T2781 mark As,present
R4250 T15365 T15362 conj lungs,diaphragm
R4251 T15366 T15365 punct ", ",lungs
R4252 T15367 T15365 cc and,lungs
R4253 T15368 T15365 conj heart,lungs
R4254 T15369 T15363 auxpass were,examined
R4255 T15370 T15349 punct .,performed
R4256 T15372 T15373 det The,lungs
R4257 T15373 T15374 nsubjpass lungs,removed
R4258 T15375 T15373 cc and,lungs
R4259 T15376 T15377 amod tracheobronchial,tree
R426 T2781 T2785 advcl present,centered
R4260 T15377 T15373 conj tree,lungs
R4261 T15378 T15374 auxpass were,removed
R4262 T15379 T15374 cc and,removed
R4263 T15380 T15374 conj weighed,removed
R4264 T15381 T15374 punct .,removed
R4265 T15383 T15384 amod Whole,diaphragms
R4266 T15384 T15385 nsubjpass diaphragms,isolated
R4267 T15386 T15385 auxpass were,isolated
R4268 T15387 T15385 prep from,isolated
R4269 T15388 T15389 amod fixed,tissue
R427 T2782 T2783 det these,patients
R4270 T15389 T15387 pobj tissue,from
R4271 T15390 T15389 amod thoracic,tissue
R4272 T15391 T15389 prep from,tissue
R4273 T15392 T15393 nmod E15.5,embryos
R4274 T15393 T15391 pobj embryos,from
R4275 T15394 T15392 cc and,E15.5
R4276 T15395 T15392 conj E17.5,E15.5
R4277 T15396 T15385 punct .,isolated
R4278 T15398 T15399 prep For,dissected
R4279 T15400 T15401 compound lung,culture
R428 T2783 T2781 nsubj patients,present
R4280 T15401 T15398 pobj culture,For
R4281 T15402 T15401 compound explant,culture
R4282 T15403 T15399 punct ", ",dissected
R4283 T15404 T15399 nsubjpass lungs,dissected
R4284 T15405 T15399 auxpass were,dissected
R4285 T15406 T15399 prep from,dissected
R4286 T15407 T15408 amod fresh,embryos
R4287 T15408 T15406 pobj embryos,from
R4288 T15409 T15399 prep at,dissected
R4289 T15410 T15409 pobj E11.5,at
R429 T2784 T2781 advmod commonly,present
R4290 T15411 T15410 cc and,E11.5
R4291 T15412 T15410 conj E12.5,E11.5
R4292 T15413 T15399 cc and,dissected
R4293 T15414 T15399 conj placed,dissected
R4294 T15415 T15414 prep on,placed
R4295 T15416 T15417 amod porous,membranes
R4296 T15417 T15415 pobj membranes,on
R4297 T15418 T15419 nummod 24,mm
R4298 T15419 T15417 nmod mm,membranes
R4299 T15420 T15419 punct -,mm
R430 T2786 T2781 prep with,present
R4300 T15421 T15422 punct (,μ
R4301 T15422 T15417 parataxis μ,membranes
R4302 T15423 T15422 nummod 0.4,μ
R4303 T15424 T15422 punct -,μ
R4304 T15425 T15422 punct ),μ
R4305 T15426 T15417 compound polyester,membranes
R4306 T15427 T15417 acl floated,membranes
R4307 T15428 T15427 prep in,floated
R4308 T15429 T15428 pobj wells,in
R4309 T15430 T15429 acl containing,wells
R431 T2787 T2788 amod severe,failure
R4310 T15431 T15432 nummod 2,ml
R4311 T15432 T15430 dobj ml,containing
R4312 T15433 T15432 prep of,ml
R4313 T15434 T15435 poss Dulbecco,medium
R4314 T15435 T15433 pobj medium,of
R4315 T15436 T15434 case 's,Dulbecco
R4316 T15437 T15435 amod modified,medium
R4317 T15438 T15435 poss Eagle,medium
R4318 T15439 T15438 case 's,Eagle
R4319 T15440 T15435 punct ", ",medium
R432 T2788 T2786 pobj failure,with
R4320 T15441 T15442 compound nutrient,mixture
R4321 T15442 T15443 compound mixture,F
R4322 T15443 T15435 appos F,medium
R4323 T15444 T15443 punct -,F
R4324 T15445 T15443 nummod 12,F
R4325 T15446 T15447 punct (,Gibco
R4326 T15447 T15443 parataxis Gibco,F
R4327 T15448 T15449 nummod 11039,021
R4328 T15449 T15447 dep 021,Gibco
R4329 T15450 T15449 punct –,021
R433 T2789 T2788 amod respiratory,failure
R4330 T15451 T15447 punct ", ",Gibco
R4331 T15452 T15447 punct ", ",Gibco
R4332 T15453 T15454 compound San,Diego
R4333 T15454 T15447 npadvmod Diego,Gibco
R4334 T15455 T15447 punct ", ",Gibco
R4335 T15456 T15447 npadvmod California,Gibco
R4336 T15457 T15447 punct ", ",Gibco
R4337 T15458 T15459 compound United,States
R4338 T15459 T15447 npadvmod States,Gibco
R4339 T15460 T15447 punct ),Gibco
R434 T2790 T2781 prep at,present
R4340 T15461 T15435 punct ", ",medium
R4341 T15462 T15435 acl supplemented,medium
R4342 T15463 T15462 prep with,supplemented
R4343 T15464 T15465 nummod 10,%
R4344 T15465 T15466 nmod %,serum
R4345 T15466 T15463 pobj serum,with
R4346 T15467 T15466 amod fetal,serum
R4347 T15468 T15466 amod bovine,serum
R4348 T15469 T15466 punct ", ",serum
R4349 T15470 T15471 nummod 0.3,mg
R435 T2791 T2790 pobj birth,at
R4350 T15471 T15472 nmod mg,glutamine
R4351 T15472 T15466 conj glutamine,serum
R4352 T15473 T15474 punct /,ml
R4353 T15474 T15471 prep ml,mg
R4354 T15475 T15472 compound L,glutamine
R4355 T15476 T15472 punct -,glutamine
R4356 T15477 T15472 punct ", ",glutamine
R4357 T15478 T15479 nummod 100,units
R4358 T15479 T15480 nmod units,penicillin
R4359 T15480 T15472 conj penicillin,glutamine
R436 T2792 T2785 punct ", ",centered
R4360 T15481 T15482 punct /,ml
R4361 T15482 T15479 prep ml,units
R4362 T15483 T15480 punct ", ",penicillin
R4363 T15484 T15485 nummod 100,mcg
R4364 T15485 T15486 nmod mcg,streptomycin
R4365 T15486 T15480 conj streptomycin,penicillin
R4366 T15487 T15488 punct /,ml
R4367 T15488 T15485 prep ml,mcg
R4368 T15489 T15486 punct ", ",streptomycin
R4369 T15490 T15486 cc and,streptomycin
R437 T2793 T2785 nsubjpass therapy,centered
R4370 T15491 T15492 nummod 0.25,mcg
R4371 T15492 T15493 nmod mcg,B
R4372 T15493 T15486 conj B,streptomycin
R4373 T15494 T15495 punct /,ml
R4374 T15495 T15492 prep ml,mcg
R4375 T15496 T15493 compound amphotericin,B
R4376 T15497 T15399 punct .,dissected
R4377 T15499 T15500 compound Lung,explants
R4378 T15500 T15501 nsubjpass explants,cultured
R4379 T15502 T15501 auxpass were,cultured
R438 T2794 T2785 aux has,centered
R4380 T15503 T15501 prep at,cultured
R4381 T15504 T15505 nummod 37,°C
R4382 T15505 T15503 pobj °C,at
R4383 T15506 T15501 prep in,cultured
R4384 T15507 T15508 nummod 95,%
R4385 T15508 T15509 compound %,air
R4386 T15509 T15506 pobj air,in
R4387 T15510 T15509 punct /,air
R4388 T15511 T15512 nummod 5,%
R4389 T15512 T15513 compound %,CO2
R439 T2795 T2785 auxpass been,centered
R4390 T15513 T15509 appos CO2,air
R4391 T15514 T15501 prep for,cultured
R4392 T15515 T15516 advmod up,5
R4393 T15516 T15518 nummod 5,d
R4394 T15517 T15516 quantmod to,5
R4395 T15518 T15514 pobj d,for
R4396 T15519 T15501 punct .,cultured
R4397 T15521 T15522 nsubjpass They,photographed
R4398 T15523 T15522 auxpass were,photographed
R4399 T15524 T15522 advmod daily,photographed
R440 T2796 T2785 prep around,centered
R4400 T15525 T15522 prep with,photographed
R4401 T15526 T15527 det a,microscope
R4402 T15527 T15525 pobj microscope,with
R4403 T15528 T15527 amod dissecting,microscope
R4404 T15529 T15530 punct (,MZ12.5
R4405 T15530 T15527 parataxis MZ12.5,microscope
R4406 T15531 T15530 punct ", ",MZ12.5
R4407 T15532 T15530 npadvmod Leica,MZ12.5
R4408 T15533 T15530 punct ", ",MZ12.5
R4409 T15534 T15530 npadvmod Wetzlar,MZ12.5
R441 T2797 T2796 pcomp developing,around
R4410 T15535 T15530 punct ", ",MZ12.5
R4411 T15536 T15530 npadvmod Germany,MZ12.5
R4412 T15537 T15530 punct ),MZ12.5
R4413 T15538 T15527 acl equipped,microscope
R4414 T15539 T15538 prep with,equipped
R4415 T15540 T15541 det a,camera
R4416 T15541 T15539 pobj camera,with
R4417 T15542 T15543 nmod Leica,DC500
R4418 T15543 T15541 nmod DC500,camera
R4419 T15544 T15541 amod digital,camera
R442 T2798 T2799 amod better,methods
R4420 T15545 T15522 punct .,photographed
R4421 T15547 T15548 amod Transgenic,mice
R4422 T15548 T15549 nsubjpass mice,developed
R4423 T15550 T15548 acl carrying,mice
R4424 T15551 T15552 det the,gene
R4425 T15552 T15550 dobj gene,carrying
R4426 T15553 T15552 compound lacZ,gene
R4427 T15554 T15552 acl driven,gene
R4428 T15555 T15554 agent by,driven
R4429 T15556 T15557 det the,promoter
R443 T2799 T2797 dobj methods,developing
R4430 T15557 T15555 pobj promoter,by
R4431 T15558 T15557 compound Fog2,promoter
R4432 T15559 T15549 aux have,developed
R4433 T15560 T15549 auxpass been,developed
R4434 T15561 T15549 agent by,developed
R4435 T15562 T15563 compound S.,Tevosian
R4436 T15563 T15561 pobj Tevosian,by
R4437 T15564 T15549 punct .,developed
R4438 T15566 T15567 prep In,incorporated
R4439 T15568 T15569 det these,animals
R444 T2800 T2801 aux to,provide
R4440 T15569 T15566 pobj animals,In
R4441 T15570 T15567 punct ", ",incorporated
R4442 T15571 T15572 det the,gene
R4443 T15572 T15567 nsubjpass gene,incorporated
R4444 T15573 T15572 compound lacZ,gene
R4445 T15574 T15567 auxpass is,incorporated
R4446 T15575 T15576 punct (,knocked
R4447 T15576 T15567 parataxis knocked,incorporated
R4448 T15577 T15576 punct “,knocked
R4449 T15578 T15576 punct -,knocked
R445 T2801 T2799 advcl provide,methods
R4450 T15579 T15576 prt in,knocked
R4451 T15580 T15576 punct ”,knocked
R4452 T15581 T15576 punct ),knocked
R4453 T15582 T15567 prep into,incorporated
R4454 T15583 T15584 det the,locus
R4455 T15584 T15582 pobj locus,into
R4456 T15585 T15584 compound Fog2,locus
R4457 T15586 T15587 aux to,allow
R4458 T15587 T15567 advcl allow,incorporated
R4459 T15588 T15589 compound β,galactosidase
R446 T2802 T2803 amod ventilatory,support
R4460 T15589 T15591 compound galactosidase,expression
R4461 T15590 T15589 punct -,galactosidase
R4462 T15591 T15587 dobj expression,allow
R4463 T15592 T15587 prep as,allow
R4464 T15593 T15594 det a,protein
R4465 T15594 T15592 pobj protein,as
R4466 T15595 T15594 compound fusion,protein
R4467 T15596 T15594 prep in,protein
R4468 T15597 T15596 pobj frame,in
R4469 T15598 T15597 prep with,frame
R447 T2803 T2801 dobj support,provide
R4470 T15599 T15600 det the,acids
R4471 T15600 T15598 pobj acids,with
R4472 T15601 T15600 amod first,acids
R4473 T15602 T15600 nummod 235,acids
R4474 T15603 T15600 compound amino,acids
R4475 T15604 T15600 prep of,acids
R4476 T15605 T15606 det the,protein
R4477 T15606 T15604 pobj protein,of
R4478 T15607 T15606 compound FOG2,protein
R4479 T15608 T15567 punct .,incorporated
R448 T2804 T2805 mark while,producing
R4480 T15610 T15611 det The,module
R4481 T15611 T15615 nsubjpass module,followed
R4482 T15612 T15613 compound Fog2,lacZ
R4483 T15613 T15611 compound lacZ,module
R4484 T15614 T15613 punct -,lacZ
R4485 T15616 T15615 auxpass is,followed
R4486 T15617 T15615 agent by,followed
R4487 T15618 T15619 det an,cassette
R4488 T15619 T15617 pobj cassette,by
R4489 T15620 T15621 compound ires,eGFP
R449 T2805 T2801 advcl producing,provide
R4490 T15621 T15619 compound eGFP,cassette
R4491 T15622 T15621 punct -,eGFP
R4492 T15623 T15615 punct .,followed
R4493 T15625 T15626 nsubj This,creates
R4494 T15627 T15628 det a,allele
R4495 T15628 T15626 dobj allele,creates
R4496 T15629 T15628 amod null,allele
R4497 T15630 T15628 prep of,allele
R4498 T15631 T15632 compound Fog2,gene
R4499 T15632 T15630 pobj gene,of
R450 T2806 T2805 neg not,producing
R4500 T15633 T15626 punct .,creates
R4501 T15635 T15636 det The,construct
R4502 T15636 T15642 nsubjpass construct,linearized
R4503 T15637 T15638 compound Fog2,eGFP
R4504 T15638 T15636 compound eGFP,construct
R4505 T15639 T15638 punct -,eGFP
R4506 T15640 T15638 compound LacZ,eGFP
R4507 T15641 T15638 punct -,eGFP
R4508 T15643 T15642 auxpass was,linearized
R4509 T15644 T15642 prep with,linearized
R451 T2807 T2808 amod further,injury
R4510 T15645 T15644 pobj KspI,with
R4511 T15646 T15642 cc and,linearized
R4512 T15647 T15642 conj electroporated,linearized
R4513 T15648 T15647 prep into,electroporated
R4514 T15649 T15650 det the,cells
R4515 T15650 T15648 pobj cells,into
R4516 T15651 T15650 compound CJ7,cells
R4517 T15652 T15650 compound ES,cells
R4518 T15653 T15642 punct .,linearized
R4519 T15655 T15656 det The,clone
R452 T2808 T2805 dobj injury,producing
R4520 T15656 T15659 nsubjpass clone,selected
R4521 T15657 T15658 advmod correctly,targeted
R4522 T15658 T15656 amod targeted,clone
R4523 T15660 T15659 auxpass was,selected
R4524 T15661 T15659 prep by,selected
R4525 T15662 T15663 det the,analysis
R4526 T15663 T15661 pobj analysis,by
R4527 T15664 T15665 compound Southern,blot
R4528 T15665 T15663 compound blot,analysis
R4529 T15666 T15659 cc and,selected
R453 T2809 T2808 compound lung,injury
R4530 T15667 T15659 conj injected,selected
R4531 T15668 T15667 prep into,injected
R4532 T15669 T15670 compound C57BL,6J
R4533 T15670 T15672 compound 6J,blastocysts
R4534 T15671 T15670 punct /,6J
R4535 T15672 T15668 pobj blastocysts,into
R4536 T15673 T15659 punct .,selected
R4537 T15675 T15676 compound Fog2,eGFP
R4538 T15676 T15680 compound eGFP,animals
R4539 T15677 T15676 punct -,eGFP
R454 T2810 T2785 punct .,centered
R4540 T15678 T15676 compound lacZ,eGFP
R4541 T15679 T15676 punct -,eGFP
R4542 T15680 T15681 nsubjpass animals,maintained
R4543 T15682 T15681 auxpass were,maintained
R4544 T15683 T15681 prep on,maintained
R4545 T15684 T15685 det the,background
R4546 T15685 T15683 pobj background,on
R4547 T15686 T15685 amod mixed,background
R4548 T15687 T15688 nmod C57BL,6J
R4549 T15688 T15685 nmod 6J,background
R455 T2812 T2813 amod Extracorporeal,oxygenation
R4550 T15689 T15688 punct /,6J
R4551 T15690 T15688 punct /,6J
R4552 T15691 T15688 nummod 129,6J
R4553 T15692 T15681 punct .,maintained
R4554 T15694 T15695 compound lacZ,Expression
R4555 T15695 T15696 nsubjpass Expression,analyzed
R4556 T15697 T15695 prep in,Expression
R4557 T15698 T15699 amod whole,lungs
R4558 T15699 T15697 pobj lungs,in
R4559 T15700 T15699 amod dissected,lungs
R456 T2813 T2815 nsubjpass oxygenation,used
R4560 T15701 T15699 amod embryonic,lungs
R4561 T15702 T15696 auxpass was,analyzed
R4562 T15703 T15696 prep by,analyzed
R4563 T15704 T15703 pcomp staining,by
R4564 T15705 T15704 prep for,staining
R4565 T15706 T15707 compound β,galactosidase
R4566 T15707 T15709 compound galactosidase,activity
R4567 T15708 T15707 punct -,galactosidase
R4568 T15709 T15705 pobj activity,for
R4569 T15710 T15704 prep with,staining
R457 T2814 T2813 compound membrane,oxygenation
R4570 T15711 T15712 compound X,gal
R4571 T15712 T15710 pobj gal,with
R4572 T15713 T15712 punct -,gal
R4573 T15714 T15704 prep after,staining
R4574 T15715 T15714 pobj fixation,after
R4575 T15716 T15715 prep for,fixation
R4576 T15717 T15718 nummod 30,min
R4577 T15718 T15716 pobj min,for
R4578 T15719 T15696 punct .,analyzed
R458 T2816 T2813 punct (,oxygenation
R4581 T16000 T16001 compound RT,PCR
R4582 T16002 T16001 punct -,PCR
R4583 T16003 T16001 cc and,PCR
R4584 T16004 T16005 compound sequence,analysis
R4585 T16005 T16001 conj analysis,PCR
R4586 T16006 T16001 prep in,PCR
R4587 T16007 T16008 det the,mouse
R4588 T16008 T16006 pobj mouse,in
R4589 T16009 T16001 punct .,PCR
R459 T2817 T2813 appos ECMO,oxygenation
R4590 T16011 T16012 nsubjpass RNA,extracted
R4591 T16013 T16012 auxpass was,extracted
R4592 T16014 T16012 prep by,extracted
R4593 T16015 T16016 amod standard,techniques
R4594 T16016 T16014 pobj techniques,by
R4595 T16017 T16012 prep from,extracted
R4596 T16018 T16019 amod thoracic,tissue
R4597 T16019 T16017 pobj tissue,from
R4598 T16020 T16019 amod embryonic,tissue
R4599 T16021 T16012 punct .,extracted
R460 T2818 T2813 punct ),oxygenation
R4600 T16023 T16024 compound RT,PCR
R4601 T16024 T16026 nsubjpass PCR,performed
R4602 T16025 T16024 punct -,PCR
R4603 T16027 T16026 auxpass was,performed
R4604 T16028 T16026 advcl using,performed
R4605 T16029 T16030 nummod six,sets
R4606 T16030 T16028 dobj sets,using
R4607 T16031 T16030 compound primer,sets
R4608 T16032 T16030 acl designed,sets
R4609 T16033 T16034 aux to,cover
R461 T2819 T2815 auxpass is,used
R4610 T16034 T16032 advcl cover,designed
R4611 T16035 T16036 det the,gene
R4612 T16036 T16034 dobj gene,cover
R4613 T16037 T16036 compound Fog2,gene
R4614 T16038 T16026 punct .,performed
R4615 T16040 T16041 compound RT,PCR
R4616 T16041 T16043 nsubjpass PCR,repeated
R4617 T16042 T16041 punct -,PCR
R4618 T16044 T16043 auxpass was,repeated
R4619 T16045 T16043 prep with,repeated
R462 T2820 T2815 prep in,used
R4620 T16046 T16047 amod radiolabeled,primers
R4621 T16047 T16045 pobj primers,with
R4622 T16048 T16049 aux to,amplify
R4623 T16049 T16043 advcl amplify,repeated
R4624 T16050 T16051 det an,region
R4625 T16051 T16049 dobj region,amplify
R4626 T16052 T16053 advmod abnormally,spliced
R4627 T16053 T16051 amod spliced,region
R4628 T16054 T16051 prep of,region
R4629 T16055 T16056 det the,gene
R463 T2821 T2822 det some,centers
R4630 T16056 T16054 pobj gene,of
R4631 T16057 T16058 punct (,S1
R4632 T16058 T16043 parataxis S1,repeated
R4633 T16059 T16058 compound Table,S1
R4634 T16060 T16058 punct ),S1
R4635 T16061 T16043 punct ", ",repeated
R4636 T16062 T16043 cc and,repeated
R4637 T16063 T16064 det the,product
R4638 T16064 T16065 nsubjpass product,run
R4639 T16065 T16043 conj run,repeated
R464 T2822 T2820 pobj centers,in
R4640 T16066 T16065 auxpass was,run
R4641 T16067 T16065 prep on,run
R4642 T16068 T16069 det a,gel
R4643 T16069 T16067 pobj gel,on
R4644 T16070 T16069 amod denaturing,gel
R4645 T16071 T16069 compound sequencing,gel
R4646 T16072 T16065 prep according,run
R4647 T16073 T16072 prep to,according
R4648 T16074 T16075 amod standard,techniques
R4649 T16075 T16073 pobj techniques,to
R465 T2823 T2824 aux to,provide
R4650 T16076 T16065 punct .,run
R4651 T16078 T16079 det The,product
R4652 T16079 T16083 nsubjpass product,cloned
R4653 T16080 T16081 compound RT,PCR
R4654 T16081 T16079 compound PCR,product
R4655 T16082 T16081 punct -,PCR
R4656 T16084 T16083 auxpass was,cloned
R4657 T16085 T16083 prep into,cloned
R4658 T16086 T16087 compound pCR2.1,vector
R4659 T16087 T16085 pobj vector,into
R466 T2824 T2815 advcl provide,used
R4660 T16088 T16083 advcl using,cloned
R4661 T16089 T16090 compound TOPO,Kit
R4662 T16090 T16088 dobj Kit,using
R4663 T16091 T16090 compound TA,Kit
R4664 T16092 T16090 compound Cloning,Kit
R4665 T16093 T16094 punct (,Invitrogen
R4666 T16094 T16090 parataxis Invitrogen,Kit
R4667 T16095 T16094 punct ", ",Invitrogen
R4668 T16096 T16094 npadvmod Carlsbad,Invitrogen
R4669 T16097 T16094 punct ", ",Invitrogen
R467 T2825 T2826 det an,period
R4670 T16098 T16094 npadvmod California,Invitrogen
R4671 T16099 T16094 punct ", ",Invitrogen
R4672 T16100 T16101 compound United,States
R4673 T16101 T16094 npadvmod States,Invitrogen
R4674 T16102 T16094 punct ),Invitrogen
R4675 T16103 T16083 cc and,cloned
R4676 T16104 T16083 conj sequenced,cloned
R4677 T16105 T16104 advcl using,sequenced
R4678 T16106 T16107 npadvmod gene,specific
R4679 T16107 T16109 amod specific,primers
R468 T2826 T2824 dobj period,provide
R4680 T16108 T16107 punct -,specific
R4681 T16109 T16105 dobj primers,using
R4682 T16110 T16083 punct .,cloned
R4683 T16112 T16113 compound Sequence,analysis
R4684 T16113 T16114 nsubjpass analysis,done
R4685 T16115 T16114 auxpass was,done
R4686 T16116 T16114 advcl using,done
R4687 T16117 T16116 dobj Sequencher,using
R4688 T16118 T16117 nummod 4.1,Sequencher
R4689 T16119 T16120 punct (,Codes
R469 T2827 T2826 amod extended,period
R4690 T16120 T16117 parataxis Codes,Sequencher
R4691 T16121 T16120 compound Gene,Codes
R4692 T16122 T16120 punct ", ",Codes
R4693 T16123 T16124 compound Ann,Arbor
R4694 T16124 T16120 npadvmod Arbor,Codes
R4695 T16125 T16120 punct ", ",Codes
R4696 T16126 T16120 npadvmod Michigan,Codes
R4697 T16127 T16120 punct ", ",Codes
R4698 T16128 T16129 compound United,States
R4699 T16129 T16120 npadvmod States,Codes
R470 T2828 T2826 prep of,period
R4700 T16130 T16120 punct ),Codes
R4701 T16131 T16114 punct .,done
R4702 T16289 T16290 advmod In,situ
R4703 T16290 T16291 amod situ,hybridization
R4704 T16292 T16291 punct .,hybridization
R4705 T16294 T16295 prep After,subjected
R4706 T16296 T16294 pobj dehydration,After
R4707 T16297 T16296 cc and,dehydration
R4708 T16298 T16296 conj embedding,dehydration
R4709 T16299 T16298 prep in,embedding
R471 T2829 T2830 amod cardiopulmonary,bypass
R4710 T16300 T16301 compound paraffin,wax
R4711 T16301 T16299 pobj wax,in
R4712 T16302 T16295 punct ", ",subjected
R4713 T16303 T16304 nummod 10,μ
R4714 T16304 T16306 compound μ,sections
R4715 T16305 T16304 punct -,μ
R4716 T16306 T16295 nsubjpass sections,subjected
R4717 T16307 T16295 auxpass were,subjected
R4718 T16308 T16295 prep to,subjected
R4719 T16309 T16310 amod radioactive,hybridization
R472 T2830 T2828 pobj bypass,of
R4720 T16310 T16308 pobj hybridization,to
R4721 T16311 T16312 advmod in,situ
R4722 T16312 T16310 amod situ,hybridization
R4723 T16313 T16314 mark as,described
R4724 T16314 T16295 advcl described,subjected
R4725 T16315 T16316 punct [,53
R4726 T16316 T16295 parataxis 53,subjected
R4727 T16317 T16316 punct ],53
R4728 T16318 T16295 punct .,subjected
R4729 T16320 T16321 nsubjpass Probes,prepared
R473 T2831 T2832 punct [,6
R4730 T16322 T16320 acl labeled,Probes
R4731 T16323 T16322 prep with,labeled
R4732 T16324 T16323 pobj 35S,with
R4733 T16325 T16321 auxpass were,prepared
R4734 T16326 T16321 prep by,prepared
R4735 T16327 T16328 amod run,transcription
R4736 T16328 T16326 pobj transcription,by
R4737 T16329 T16327 punct -,run
R4738 T16330 T16327 prt off,run
R4739 T16331 T16328 prep of,transcription
R474 T2832 T2815 parataxis 6,used
R4740 T16332 T16333 amod linearized,templates
R4741 T16333 T16331 pobj templates,of
R4742 T16334 T16333 compound plasmid,templates
R4743 T16335 T16321 cc and,prepared
R4744 T16336 T16321 conj hybridized,prepared
R4745 T16337 T16336 prep to,hybridized
R4746 T16338 T16339 compound tissue,sections
R4747 T16339 T16337 pobj sections,to
R4748 T16340 T16321 punct .,prepared
R4749 T16342 T16343 nsubjpass Nuclei,counterstained
R475 T2833 T2832 nummod 5,6
R4750 T16344 T16343 auxpass were,counterstained
R4751 T16345 T16343 prep with,counterstained
R4752 T16346 T16345 pobj Hoescht,with
R4753 T16347 T16346 nummod 33258,Hoescht
R4754 T16348 T16343 punct ", ",counterstained
R4755 T16349 T16343 cc and,counterstained
R4756 T16350 T16351 nsubjpass signal,imaged
R4757 T16351 T16343 conj imaged,counterstained
R4758 T16352 T16351 auxpass was,imaged
R4759 T16353 T16351 advcl using,imaged
R476 T2834 T2832 punct ",",6
R4760 T16354 T16355 amod fluorescent,microscopy
R4761 T16355 T16353 dobj microscopy,using
R4762 T16356 T16354 cc and,fluorescent
R4763 T16357 T16354 conj darkfield,fluorescent
R4764 T16358 T16351 punct .,imaged
R4765 T16688 T16689 amod Human,extraction
R4766 T16690 T16689 compound DNA,extraction
R4767 T16691 T16689 cc and,extraction
R4768 T16692 T16693 compound sequence,analysis
R4769 T16693 T16689 conj analysis,extraction
R477 T2835 T2832 punct ],6
R4770 T16694 T16693 punct .,analysis
R4771 T16696 T16697 nsubjpass DNA,isolated
R4772 T16698 T16697 auxpass was,isolated
R4773 T16699 T16697 prep from,isolated
R4774 T16700 T16701 compound paraffin,blocks
R4775 T16701 T16699 pobj blocks,from
R4776 T16702 T16697 prep by,isolated
R4777 T16703 T16704 compound phenol,chloroform
R4778 T16704 T16706 compound chloroform,extraction
R4779 T16705 T16704 punct -,chloroform
R478 T2836 T2815 punct ", ",used
R4780 T16706 T16702 pobj extraction,by
R4781 T16707 T16708 punct [,55
R4782 T16708 T16697 parataxis 55,isolated
R4783 T16709 T16708 nummod 54,55
R4784 T16710 T16708 punct ",",55
R4785 T16711 T16708 punct ],55
R4786 T16712 T16697 punct ", ",isolated
R4787 T16713 T16697 cc and,isolated
R4788 T16714 T16697 conj from,isolated
R4789 T16715 T16716 amod frozen,tissues
R479 T2837 T2838 mark while,have
R4790 T16716 T16714 pobj tissues,from
R4791 T16717 T16714 prep by,from
R4792 T16718 T16719 amod standard,techniques
R4793 T16719 T16717 pobj techniques,by
R4794 T16720 T16697 punct .,isolated
R4795 T16722 T16723 nsubjpass Primers,designed
R4796 T16724 T16723 auxpass were,designed
R4797 T16725 T16726 aux to,amplify
R4798 T16726 T16723 advcl amplify,designed
R4799 T16727 T16728 compound FOG2,exons
R480 T2838 T2815 advcl have,used
R4800 T16728 T16726 dobj exons,amplify
R4801 T16729 T16728 compound coding,exons
R4802 T16730 T16728 cc plus,exons
R4803 T16731 T16732 nummod 50,bp
R4804 T16732 T16728 conj bp,exons
R4805 T16733 T16732 prep of,bp
R4806 T16734 T16735 amod flanking,sequence
R4807 T16735 T16733 pobj sequence,of
R4808 T16736 T16735 amod upstream,sequence
R4809 T16737 T16736 cc and,upstream
R481 T2839 T2840 amod other,centers
R4810 T16738 T16736 conj downstream,upstream
R4811 T16739 T16723 punct .,designed
R4812 T16741 T16742 compound PCR,amplification
R4813 T16742 T16743 nsubjpass amplification,performed
R4814 T16744 T16742 cc and,amplification
R4815 T16745 T16742 conj sequencing,amplification
R4816 T16746 T16743 auxpass were,performed
R4817 T16747 T16743 prep by,performed
R4818 T16748 T16749 amod standard,methods
R4819 T16749 T16747 pobj methods,by
R482 T2840 T2838 nsubj centers,have
R4820 T16750 T16743 punct .,performed
R4821 T16752 T16753 compound Primer,sequences
R4822 T16753 T16754 nsubjpass sequences,listed
R4823 T16755 T16753 acl used,sequences
R4824 T16756 T16754 auxpass are,listed
R4825 T16757 T16754 prep in,listed
R4826 T16758 T16759 compound Table,S2
R4827 T16759 T16757 pobj S2,in
R4828 T16760 T16754 punct .,listed
R4829 T16762 T16763 compound Sequence,analysis
R483 T2841 T2838 dobj success,have
R4830 T16763 T16764 nsubjpass analysis,done
R4831 T16765 T16764 auxpass was,done
R4832 T16766 T16764 prep with,done
R4833 T16767 T16766 pobj Sequencher,with
R4834 T16768 T16767 nummod 4.1,Sequencher
R4835 T16769 T16770 punct (,Codes
R4836 T16770 T16767 parataxis Codes,Sequencher
R4837 T16771 T16770 compound Gene,Codes
R4838 T16772 T16770 punct ),Codes
R4839 T16773 T16764 punct .,done
R484 T2842 T2838 advcl using,have
R4840 T16775 T16776 nsubjpass DNA,extracted
R4841 T16777 T16775 prep from,DNA
R4842 T16778 T16779 det the,parents
R4843 T16779 T16777 pobj parents,from
R4844 T16780 T16779 prep of,parents
R4845 T16781 T16782 nummod one,patient
R4846 T16782 T16780 pobj patient,of
R4847 T16783 T16782 compound autopsy,patient
R4848 T16784 T16776 auxpass was,extracted
R4849 T16785 T16776 prep from,extracted
R485 T2843 T2844 amod other,techniques
R4850 T16786 T16787 amod fresh,samples
R4851 T16787 T16785 pobj samples,from
R4852 T16788 T16787 compound blood,samples
R4853 T16789 T16776 punct .,extracted
R4854 T16791 T16792 det A,set
R4855 T16792 T16794 nsubjpass set,sent
R4856 T16793 T16792 amod second,set
R4857 T16795 T16792 prep of,set
R4858 T16796 T16797 compound blood,samples
R4859 T16797 T16795 pobj samples,of
R486 T2844 T2842 dobj techniques,using
R4860 T16798 T16794 auxpass was,sent
R4861 T16799 T16794 prep to,sent
R4862 T16800 T16801 det an,laboratory
R4863 T16801 T16799 pobj laboratory,to
R4864 T16802 T16801 amod outside,laboratory
R4865 T16803 T16804 npadvmod CLIA,certified
R4866 T16804 T16801 amod certified,laboratory
R4867 T16805 T16804 punct -,certified
R4868 T16806 T16794 prep for,sent
R4869 T16807 T16808 compound DNA,extraction
R487 T2845 T2846 amod ventilatory,support
R4870 T16808 T16806 pobj extraction,for
R4871 T16809 T16808 punct ", ",extraction
R4872 T16810 T16808 conj PCR,extraction
R4873 T16811 T16810 punct ", ",PCR
R4874 T16812 T16810 conj sequencing,PCR
R4875 T16813 T16812 punct ", ",sequencing
R4876 T16814 T16812 cc and,sequencing
R4877 T16815 T16812 conj analysis,sequencing
R4878 T16816 T16794 punct .,sent
R4879 T16818 T16819 compound Paternity,testing
R488 T2846 T2844 compound support,techniques
R4880 T16819 T16820 nsubjpass testing,performed
R4881 T16821 T16820 auxpass was,performed
R4882 T16822 T16820 agent by,performed
R4883 T16823 T16824 det the,laboratory
R4884 T16824 T16822 pobj laboratory,by
R4885 T16825 T16824 amod outside,laboratory
R4886 T16826 T16820 advcl using,performed
R4887 T16827 T16828 det a,panel
R4888 T16828 T16826 dobj panel,using
R4889 T16829 T16828 amod standard,panel
R489 T2847 T2848 punct [,7
R4890 T16830 T16828 prep of,panel
R4891 T16831 T16830 pobj markers,of
R4892 T16832 T16820 punct .,performed
R4893 T16834 T16835 compound SNP,genotyping
R4894 T16835 T16836 nsubjpass genotyping,done
R4895 T16837 T16836 auxpass was,done
R4896 T16838 T16836 advcl using,done
R4897 T16839 T16840 nmod Harvard,Center
R4898 T16840 T16842 nmod Center,facility
R4899 T16841 T16840 nmod Partners,Center
R490 T2848 T2838 parataxis 7,have
R4900 T16842 T16838 dobj facility,using
R4901 T16843 T16840 prep for,Center
R4902 T16844 T16843 pobj Genetics,for
R4903 T16845 T16844 cc and,Genetics
R4904 T16846 T16844 conj Genomics,Genetics
R4905 T16847 T16842 compound genotyping,facility
R4906 T16848 T16842 compound core,facility
R4907 T16849 T16850 punct (,Cambridge
R4908 T16850 T16842 parataxis Cambridge,facility
R4909 T16851 T16850 punct ", ",Cambridge
R491 T2849 T2848 punct ],7
R4910 T16852 T16850 npadvmod Massachusetts,Cambridge
R4911 T16853 T16850 punct ", ",Cambridge
R4912 T16854 T16855 compound United,States
R4913 T16855 T16850 npadvmod States,Cambridge
R4914 T16856 T16850 punct ),Cambridge
R4915 T16857 T16836 punct .,done
R492 T2850 T2815 punct .,used
R493 T2852 T2853 det The,morbidity
R494 T2853 T2854 nsubj morbidity,is
R495 T2855 T2853 prep in,morbidity
R496 T2856 T2855 pobj those,in
R497 T2857 T2858 dep who,survive
R498 T2858 T2856 relcl survive,those
R499 T2859 T2854 acomp high,is
R500 T2860 T2854 punct ", ",is
R501 T2861 T2854 cc and,is
R502 T2862 T2863 amod many,patients
R503 T2863 T2864 nsubj patients,survive
R504 T2864 T2854 conj survive,is
R505 T2865 T2864 prep with,survive
R506 T2866 T2867 amod chronic,insufficiency
R507 T2867 T2865 pobj insufficiency,with
R508 T2868 T2867 amod respiratory,insufficiency
R509 T2869 T2867 punct ", ",insufficiency
R510 T2870 T2871 amod cognitive,deficits
R511 T2871 T2867 conj deficits,insufficiency
R512 T2872 T2870 cc and,cognitive
R513 T2873 T2870 conj neuromotor,cognitive
R514 T2874 T2871 punct ", ",deficits
R515 T2875 T2871 cc and,deficits
R516 T2876 T2877 compound hearing,loss
R517 T2877 T2871 conj loss,deficits
R518 T2878 T2864 prep as,survive
R519 T2879 T2880 det a,result
R520 T2880 T2878 pobj result,as
R521 T2881 T2880 prep of,result
R522 T2882 T2883 amod necessary,interventions
R523 T2883 T2881 pobj interventions,of
R524 T2884 T2883 amod intensive,interventions
R525 T2885 T2883 cc and,interventions
R526 T2886 T2887 amod associated,abnormalities
R527 T2887 T2883 conj abnormalities,interventions
R528 T2888 T2887 amod structural,abnormalities
R529 T2889 T2888 cc and,structural
R530 T2890 T2888 conj irreversible,structural
R531 T2891 T2887 amod developmental,abnormalities
R532 T2892 T2893 punct [,8
R533 T2893 T2864 parataxis 8,survive
R534 T2894 T2895 punct –,11
R535 T2895 T2893 prep 11,8
R536 T2896 T2893 punct ],8
R537 T2897 T2864 punct .,survive
R538 T2899 T2900 prep To,been
R539 T2901 T2899 pobj date,To
R540 T2902 T2900 punct ", ",been
R541 T2903 T2900 expl there,been
R542 T2904 T2900 aux have,been
R543 T2905 T2906 det no,mutations
R544 T2906 T2900 attr mutations,been
R545 T2907 T2906 amod specific,mutations
R546 T2908 T2906 acl found,mutations
R547 T3015 T2998 xcomp develop,is
R548 T3016 T3017 compound animal,models
R549 T3017 T3015 dobj models,develop
R550 T3018 T3017 prep of,models
R551 T3019 T3020 amod human,defects
R552 T3020 T3018 pobj defects,of
R553 T3021 T3020 compound birth,defects
R554 T3022 T3015 punct ", ",develop
R555 T3023 T3024 mark as,investigated
R556 T3024 T3015 advcl investigated,develop
R557 T3025 T3026 det the,abnormalities
R558 T3026 T3024 nsubjpass abnormalities,investigated
R559 T2909 T2910 aux to,be
R560 T3027 T3026 amod specific,abnormalities
R561 T2910 T2908 xcomp be,found
R562 T3028 T3026 amod genetic,abnormalities
R563 T2911 T2910 acomp associated,be
R564 T2912 T2911 prep with,associated
R565 T3029 T3026 acl found,abnormalities
R566 T2913 T2914 amod nonsyndromic,defects
R567 T2914 T2912 pobj defects,with
R568 T2915 T2914 amod diaphragmatic,defects
R569 T3030 T3029 prep in,found
R570 T2916 T2914 cc and,defects
R571 T2917 T2918 amod pulmonary,hypoplasia
R572 T3031 T3032 compound animal,models
R573 T2918 T2914 conj hypoplasia,defects
R574 T2919 T2910 prep in,be
R575 T2920 T2919 pobj humans,in
R576 T3032 T3030 pobj models,in
R577 T2921 T2900 punct .,been
R578 T3033 T3024 aux can,investigated
R579 T2923 T2924 det The,heritability
R580 T2924 T2925 nsubj heritability,is
R581 T3034 T3024 advmod then,investigated
R582 T2926 T2924 prep of,heritability
R583 T2927 T2928 det these,defects
R584 T3035 T3024 auxpass be,investigated
R585 T2928 T2926 pobj defects,of
R586 T2929 T2925 acomp unclear,is
R587 T3036 T3024 prep in,investigated
R588 T3037 T3038 amod human,populations
R589 T2930 T2925 punct ", ",is
R590 T2931 T2932 mark as,limit
R591 T3038 T3036 pobj populations,in
R592 T2932 T2925 advcl limit,is
R593 T2933 T2934 det the,morbidity
R594 T3039 T2998 punct .,is
R595 T2934 T2932 nsubj morbidity,limit
R596 T2935 T2934 amod high,morbidity
R597 T2936 T2934 cc and,morbidity
R598 T3041 T3042 nsubj We,screened
R599 T2937 T2934 conj mortality,morbidity
R600 T2938 T2939 det the,collection
R601 T2939 T2932 dobj collection,limit
R602 T2940 T2939 prep of,collection
R603 T3043 T3042 dobj mice,screened
R604 T2941 T2942 amod multigenerational,families
R605 T2942 T2940 pobj families,of
R606 T2943 T2939 prep for,collection
R607 T2944 T2943 pobj analysis,for
R608 T2945 T2925 punct .,is
R609 T3044 T3043 acl treated,mice
R610 T2947 T2948 det The,etiologies
R611 T2948 T2950 nsubj etiologies,are
R612 T2949 T2948 amod genetic,etiologies
R613 T3045 T3044 prep with,treated
R614 T2951 T2950 acomp likely,are
R615 T2952 T2953 aux to,be
R616 T3046 T3047 det the,mutagen
R617 T2953 T2951 xcomp be,likely
R618 T2954 T2953 acomp complex,be
R619 T3047 T3045 pobj mutagen,with
R620 T2955 T2950 cc and,are
R621 T2956 T2957 advmod probably,arise
R622 T2957 T2950 conj arise,are
R623 T3048 T3047 amod chemical,mutagen
R624 T2958 T2957 prep from,arise
R625 T2959 T2960 amod different,mutations
R626 T2960 T2958 pobj mutations,from
R627 T3049 T3050 compound N,ethyl
R628 T2961 T2957 prep in,arise
R629 T2962 T2963 amod various,parts
R630 T2963 T2961 pobj parts,in
R631 T2964 T2963 prep of,parts
R632 T3050 T3047 appos ethyl,mutagen
R633 T2965 T2966 det the,pathways
R634 T2966 T2964 pobj pathways,of
R635 T3051 T3050 punct -,ethyl
R636 T2967 T2966 amod molecular,pathways
R637 T2968 T2966 amod developmental,pathways
R638 T2969 T2966 acl required,pathways
R639 T3052 T3050 punct -,ethyl
R640 T2970 T2969 prep for,required
R641 T2971 T2972 amod diaphragmatic,development
R642 T2972 T2970 pobj development,for
R643 T3053 T3054 compound N,nitrosourea
R644 T2973 T2950 punct .,are
R645 T3054 T3050 appos nitrosourea,ethyl
R646 T2975 T2976 advmod Indeed,are
R647 T3055 T3054 punct -,nitrosourea
R648 T2977 T2976 punct ", ",are
R649 T2978 T2976 expl there,are
R650 T2979 T2980 amod numerous,reports
R651 T3056 T3050 punct (,ethyl
R652 T2980 T2976 attr reports,are
R653 T2981 T2980 acl implicating,reports
R654 T2982 T2983 amod different,abnormalities
R655 T2983 T2981 dobj abnormalities,implicating
R656 T3057 T3050 appos ENU,ethyl
R657 T2984 T2983 amod chromosomal,abnormalities
R658 T2985 T2981 prep in,implicating
R659 T2986 T2987 det the,pathogenesis
R660 T2987 T2985 pobj pathogenesis,in
R661 T2988 T2987 prep of,pathogenesis
R662 T3058 T3042 punct ),screened
R663 T2989 T2988 pobj CDH,of
R664 T2990 T2991 punct [,13
R665 T2991 T2976 parataxis 13,are
R666 T3059 T3042 prep for,screened
R667 T2992 T2991 nummod 12,13
R668 T2993 T2991 punct ",",13
R669 T2994 T2991 punct ],13
R670 T3060 T3059 pobj lines,for
R671 T2995 T2976 punct .,are
R672 T3061 T3060 prep with,lines
R673 T2997 T2998 prep Given,is
R674 T2999 T3000 det the,difficulty
R675 T3062 T3063 amod developmental,defects
R676 T3000 T2997 pobj difficulty,Given
R677 T3001 T3000 prep of,difficulty
R678 T3002 T3001 pcomp studying,of
R679 T3063 T3061 pobj defects,with
R680 T3003 T3004 amod lethal,abnormalities
R681 T3004 T3002 dobj abnormalities,studying
R682 T3064 T3065 dep that,present
R683 T3005 T3004 amod developmental,abnormalities
R684 T3006 T3002 prep in,studying
R685 T3007 T3006 pobj humans,in
R686 T3065 T3063 relcl present,defects
R687 T3008 T2998 punct ", ",is
R688 T3009 T2998 nsubj it,is
R689 T3010 T2998 prep of,is
R690 T3066 T3065 prep in,present
R691 T3011 T3012 amod great,utility
R692 T3012 T3010 pobj utility,of
R693 T3067 T3068 det the,period
R694 T3013 T3012 amod potential,utility
R695 T3014 T3015 aux to,develop
R696 T3068 T3066 pobj period,in
R697 T3069 T3068 amod perinatal,period
R698 T3070 T3071 punct [,14
R699 T3071 T3042 parataxis 14,screened
R700 T3072 T3071 punct ],14
R701 T3073 T3042 punct .,screened
R702 T3075 T3076 prep From,identified
R703 T3121 T3119 dobj mutation,revealed
R704 T3122 T3119 prep in,revealed
R705 T3123 T3124 det a,site
R706 T3124 T3122 pobj site,in
R707 T3125 T3124 compound splice,site
R708 T3126 T3124 compound donor,site
R709 T3077 T3078 det this,screen
R710 T3127 T3128 dep that,generates
R711 T3128 T3124 relcl generates,site
R712 T3129 T3130 det an,transcript
R713 T3130 T3128 dobj transcript,generates
R714 T3131 T3130 amod abnormal,transcript
R715 T3078 T3075 pobj screen,From
R716 T3132 T3130 acl encoding,transcript
R717 T3133 T3134 det a,protein
R718 T3134 T3132 dobj protein,encoding
R719 T3079 T3076 punct ", ",identified
R720 T3135 T3134 amod truncated,protein
R721 T3136 T3119 punct .,revealed
R722 T3080 T3076 nsubj we,identified
R723 T3138 T3139 det This,result
R724 T3139 T3140 nsubj result,suggested
R725 T3081 T3082 det a,line
R726 T3141 T3142 mark that,examine
R727 T3142 T3140 ccomp examine,suggested
R728 T3082 T3076 dobj line,identified
R729 T3143 T3142 nsubj we,examine
R730 T3144 T3145 det the,gene
R731 T3083 T3082 prep of,line
R732 T3145 T3142 dobj gene,examine
R733 T3146 T3145 amod orthologous,gene
R734 T3147 T3142 prep in,examine
R735 T3084 T3083 pobj mice,of
R736 T3148 T3147 pobj humans,in
R737 T3149 T3148 prep with,humans
R738 T3150 T3151 amod similar,defects
R739 T3085 T3084 acl carrying,mice
R740 T3151 T3149 pobj defects,with
R741 T3152 T3151 amod developmental,defects
R742 T3086 T3087 det a,mutation
R743 T3153 T3140 punct ", ",suggested
R744 T3154 T3140 cc and,suggested
R745 T3155 T3156 nsubj we,report
R746 T3087 T3085 dobj mutation,carrying
R747 T3156 T3140 conj report,suggested
R748 T3157 T3158 det the,finding
R749 T3088 T3087 amod recessive,mutation
R750 T3158 T3156 dobj finding,report
R751 T3159 T3158 prep of,finding
R752 T3160 T3161 det a,mutation
R753 T3089 T3090 dep that,results
R754 T3161 T3159 pobj mutation,of
R755 T3162 T3163 advmod de,novo
R756 T3163 T3161 amod novo,mutation
R757 T3164 T3161 compound nonsense,mutation
R758 T3165 T3158 prep in,finding
R759 T3166 T3165 pobj FOG2,in
R760 T3090 T3087 relcl results,mutation
R761 T3167 T3158 prep in,finding
R762 T3168 T3169 det a,patient
R763 T3091 T3090 prep in,results
R764 T3169 T3167 pobj patient,in
R765 T3170 T3171 dep who,died
R766 T3171 T3169 relcl died,patient
R767 T3172 T3171 prep at,died
R768 T3092 T3093 amod primary,hypoplasia
R769 T3173 T3172 pobj birth,at
R770 T3174 T3171 prep with,died
R771 T3093 T3091 pobj hypoplasia,in
R772 T3175 T3176 det a,defect
R773 T3176 T3174 pobj defect,with
R774 T3177 T3176 amod diaphragmatic,defect
R775 T3178 T3176 cc and,defect
R776 T3094 T3093 amod pulmonary,hypoplasia
R777 T3179 T3180 amod severe,hypoplasia
R778 T3180 T3176 conj hypoplasia,defect
R779 T3181 T3180 amod pulmonary,hypoplasia
R780 T3095 T3093 cc and,hypoplasia
R781 T3182 T3156 punct .,report
R782 T3096 T3097 amod abnormal,development
R783 T3184 T3185 nsubj This,is
R784 T3186 T3187 det the,mutation
R785 T3097 T3093 conj development,hypoplasia
R786 T3098 T3097 amod diaphragmatic,development
R787 T3099 T3098 cc and,diaphragmatic
R788 T3187 T3185 attr mutation,is
R789 T3100 T3098 conj cardiac,diaphragmatic
R790 T3188 T3187 amod first,mutation
R791 T3189 T3187 amod reported,mutation
R792 T3190 T3187 amod associated,mutation
R793 T3101 T3076 punct .,identified
R794 T3191 T3190 prep with,associated
R795 T3192 T3193 det these,abnormalities
R796 T3193 T3191 pobj abnormalities,with
R797 T3103 T3104 amod Positional,analysis
R798 T3194 T3185 prep in,is
R799 T3195 T3196 det a,human
R800 T3196 T3194 pobj human,in
R801 T3197 T3185 punct .,is
R802 T3104 T3106 nsubj analysis,identified
R803 T3199 T3200 nsubj We,present
R804 T3105 T3104 compound cloning,analysis
R805 T3201 T3202 amod additional,data
R806 T3202 T3200 dobj data,present
R807 T3107 T3106 dobj Fog2,identified
R808 T3203 T3204 dep that,provide
R809 T3204 T3202 relcl provide,data
R810 T3205 T3206 amod direct,evidence
R811 T3206 T3204 dobj evidence,provide
R812 T3207 T3208 mark that,be
R813 T3208 T3206 acl be,evidence
R814 T3108 T3109 punct (,Zfpm2
R815 T3209 T3210 amod pulmonary,hypoplasia
R816 T3210 T3208 nsubj hypoplasia,be
R817 T3109 T3107 parataxis Zfpm2,Fog2
R818 T3211 T3208 aux may,be
R819 T3212 T3213 det a,component
R820 T3213 T3208 attr component,be
R821 T3110 T3109 punct ),Zfpm2
R822 T3214 T3213 amod primary,component
R823 T3215 T3213 prep of,component
R824 T3216 T3217 det this,spectrum
R825 T3111 T3106 prep as,identified
R826 T3217 T3215 pobj spectrum,of
R827 T3218 T3217 prep of,spectrum
R828 T3219 T3218 pobj disorders,of
R829 T3112 T3113 det a,candidate
R830 T3220 T3200 punct .,present
R831 T3113 T3111 pobj candidate,as
R832 T3114 T3113 amod likely,candidate
R833 T3115 T3106 punct ", ",identified
R834 T3116 T3106 cc and,identified
R835 T3117 T3118 compound DNA,sequencing
R836 T3118 T3119 nsubj sequencing,revealed
R837 T3119 T3106 conj revealed,identified
R838 T3120 T3121 det a,mutation
R839 T5357 T5356 prep of,Identification
R840 T5358 T5359 det the,Mutation
R841 T5359 T5357 pobj Mutation,of
R842 T5360 T5361 amod little,lung
R843 T5361 T5359 compound lung,Mutation
R844 T5362 T5356 prep in,Identification
R845 T5363 T5362 pobj Fog2,in
R846 T5365 T5366 nsubj We,screened
R847 T5367 T5368 amod third,generation
R848 T5368 T5370 compound generation,progeny
R849 T5369 T5368 punct -,generation
R850 T5370 T5366 dobj progeny,screened
R851 T5371 T5370 prep of,progeny
R852 T5372 T5373 npadvmod ENU,mutagenized
R853 T5373 T5375 amod mutagenized,mice
R854 T5374 T5373 punct -,mutagenized
R855 T5375 T5371 pobj mice,of
R856 T5376 T5366 prep at,screened
R857 T5377 T5378 amod embryonic,day
R858 T5378 T5376 pobj day,at
R859 T5379 T5378 nummod 18.5,day
R860 T5380 T5378 punct (,day
R861 T5381 T5378 appos E18.5,day
R862 T5382 T5366 punct ),screened
R863 T5383 T5366 prep for,screened
R864 T5384 T5385 amod abnormal,phenotypes
R865 T5385 T5383 pobj phenotypes,for
R866 T5386 T5385 amod developmental,phenotypes
R867 T5387 T5388 punct [,14
R868 T5388 T5366 parataxis 14,screened
R869 T5389 T5388 punct ],14
R870 T5390 T5366 punct .,screened
R871 T5392 T5393 nummod One,line
R872 T5393 T5394 nsubjpass line,found
R873 T5395 T5393 prep of,line
R874 T5396 T5395 pobj mice,of
R875 T5397 T5394 auxpass was,found
R876 T5398 T5399 aux to,have
R877 T5399 T5394 xcomp have,found
R878 T5400 T5401 amod multiple,embryos
R879 T5401 T5399 dobj embryos,have
R880 T5402 T5401 prep in,embryos
R881 T5403 T5404 amod independent,litters
R882 T5404 T5402 pobj litters,in
R883 T5405 T5406 dep that,displayed
R884 T5406 T5401 relcl displayed,embryos
R885 T5407 T5408 amod pulmonary,hypoplasia
R886 T5408 T5406 dobj hypoplasia,displayed
R887 T5409 T5408 cc and,hypoplasia
R888 T5410 T5411 det a,diaphragm
R889 T5411 T5408 conj diaphragm,hypoplasia
R890 T5412 T5411 amod thin,diaphragm
R891 T5413 T5394 punct .,found
R892 T5415 T5416 det The,mutation
R893 T5416 T5417 nsubjpass mutation,mapped
R894 T5418 T5416 punct ", ",mutation
R895 T5419 T5420 dep which,called
R896 T5420 T5416 relcl called,mutation
R897 T5421 T5420 nsubj we,called
R898 T5422 T5423 amod little,lung
R899 T5423 T5420 oprd lung,called
R900 T5424 T5423 punct (,lung
R901 T5425 T5423 appos lil,lung
R902 T5426 T5423 punct ),lung
R903 T5427 T5417 punct ", ",mapped
R904 T5428 T5417 auxpass was,mapped
R905 T5429 T5417 prep to,mapped
R906 T5430 T5429 pobj Chromosome,to
R907 T5431 T5430 nummod 15,Chromosome
R908 T5432 T5417 prep by,mapped
R909 T5433 T5432 pcomp utilizing,by
R910 T5434 T5435 det a,strategy
R911 T5435 T5433 dobj strategy,utilizing
R912 T5442 T5441 nsubj data,shown
R913 T5436 T5435 prep of,strategy
R914 T5443 T5441 neg not,shown
R915 T5444 T5441 punct ),shown
R916 T5437 T5438 amod interval,analysis
R917 T5445 T5446 punct [,15
R918 T5446 T5417 parataxis 15,mapped
R919 T5438 T5436 pobj analysis,of
R920 T5447 T5446 punct ],15
R921 T5448 T5417 punct .,mapped
R922 T5439 T5438 compound haplotype,analysis
R923 T5450 T5451 prep For,analyzed
R924 T5440 T5441 punct (,shown
R925 T5452 T5453 amod high,resolution
R926 T5441 T5417 parataxis shown,mapped
R927 T5453 T5455 compound resolution,mapping
R928 T5454 T5453 punct -,resolution
R929 T5455 T5450 pobj mapping,For
R930 T5548 T5549 compound flanking,markers
R931 T5456 T5451 punct ", ",analyzed
R932 T5457 T5458 compound F2,progeny
R933 T5458 T5451 nsubjpass progeny,analyzed
R934 T5549 T5544 dobj markers,D15Mit5
R935 T5459 T5458 prep from,progeny
R936 T5460 T5461 nummod two,crosses
R937 T5461 T5459 pobj crosses,from
R938 T5550 T5535 punct .,established
R939 T5462 T5451 auxpass were,analyzed
R940 T5463 T5451 punct .,analyzed
R941 T5465 T5466 prep In,defined
R942 T5552 T5553 det The,phenotype
R943 T5467 T5468 nummod 450,progeny
R944 T5468 T5465 pobj progeny,In
R945 T5469 T5468 prep of,progeny
R946 T5470 T5471 det an,intercross
R947 T5553 T5555 nsubjpass phenotype,identified
R948 T5471 T5469 pobj intercross,of
R949 T5472 T5471 prep of,intercross
R950 T5473 T5474 nmod F1,mice
R951 T5554 T5553 compound lil,phenotype
R952 T5474 T5472 pobj mice,of
R953 T5475 T5476 punct (,J
R954 T5556 T5555 auxpass was,identified
R955 T5476 T5474 nmod J,mice
R956 T5477 T5476 nmod A,J
R957 T5478 T5476 punct /,J
R958 T5479 T5476 punct ×,J
R959 T5480 T5481 compound FVB,N
R960 T5481 T5476 appos N,J
R961 T5557 T5555 prep at,identified
R962 T5482 T5481 punct /,N
R963 T5483 T5476 punct ),J
R964 T5484 T5474 nmod lil,mice
R965 T5485 T5484 punct /,lil
R966 T5558 T5557 pobj E18.5,at
R967 T5486 T5484 punct +,lil
R968 T5487 T5466 punct ", ",defined
R969 T5488 T5489 det the,interval
R970 T5559 T5560 aux to,have
R971 T5489 T5466 nsubjpass interval,defined
R972 T5490 T5489 acl containing,interval
R973 T5491 T5492 det the,mutation
R974 T5560 T5555 xcomp have,identified
R975 T5492 T5490 dobj mutation,containing
R976 T5493 T5466 auxpass was,defined
R977 T5494 T5466 prep by,defined
R978 T5561 T5562 amod bilateral,hypoplasia
R979 T5495 T5496 nummod 19,recombinants
R980 T5496 T5494 pobj recombinants,by
R981 T5497 T5496 prep between,recombinants
R982 T5562 T5560 dobj hypoplasia,have
R983 T5498 T5497 pobj D15Mit220,between
R984 T5499 T5498 cc and,D15Mit220
R985 T5500 T5498 conj D15Mit154,D15Mit220
R986 T5563 T5562 amod pulmonary,hypoplasia
R987 T5501 T5466 punct .,defined
R988 T5564 T5562 cc and,hypoplasia
R989 T5503 T5504 prep Because,tested
R990 T5505 T5503 pcomp of,Because
R991 T5506 T5507 det the,lack
R992 T5565 T5566 det an,diaphragm
R993 T5507 T5503 pobj lack,Because
R994 T5508 T5507 prep of,lack
R995 T5509 T5510 amod informative,markers
R996 T5510 T5508 pobj markers,of
R997 T5566 T5562 conj diaphragm,hypoplasia
R998 T5567 T5566 amod abnormal,diaphragm
R999 T5511 T5507 prep within,lack