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PMC:1183529 / 28032-32579 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T14396 293-300 JJ denotes Genetic
T14397 301-308 NN denotes mapping
T14398 309-311 IN denotes of
T14399 312-315 DT denotes the
T14401 316-321 NN denotes mouse
T14400 322-330 NN denotes mutation
T14402 331-334 NN denotes lil
T14403 334-335 . denotes .
T14404 335-392 sentence denotes The lil mutation was identified as described in results.
T14405 336-339 DT denotes The
T14407 340-343 NN denotes lil
T14406 344-352 NN denotes mutation
T14409 353-356 VBD denotes was
T14408 357-367 VBN denotes identified
T14410 368-370 IN denotes as
T14411 371-380 VBN denotes described
T14412 381-383 IN denotes in
T14413 384-391 NNS denotes results
T14414 391-392 . denotes .
T14415 392-529 sentence denotes Wild-type FVB/N and C57BL/6J mice used for genetic crosses were obtained from the Jackson Laboratory (Bar Harbor, Maine, United States).
T14416 393-397 JJ denotes Wild
T14418 397-398 HYPH denotes -
T14417 398-402 NN denotes type
T14420 403-406 NN denotes FVB
T14422 406-407 HYPH denotes /
T14421 407-408 NN denotes N
T14423 409-412 CC denotes and
T14424 413-418 NN denotes C57BL
T14426 418-419 HYPH denotes /
T14425 419-421 NN denotes 6J
T14419 422-426 NNS denotes mice
T14428 427-431 VBN denotes used
T14429 432-435 IN denotes for
T14430 436-443 JJ denotes genetic
T14431 444-451 NNS denotes crosses
T14432 452-456 VBD denotes were
T14427 457-465 VBN denotes obtained
T14433 466-470 IN denotes from
T14434 471-474 DT denotes the
T14436 475-482 NNP denotes Jackson
T14435 483-493 NNP denotes Laboratory
T14437 494-495 -LRB- denotes (
T14439 495-498 NNP denotes Bar
T14438 499-505 NNP denotes Harbor
T14440 505-507 , denotes ,
T14441 507-512 NNP denotes Maine
T14442 512-514 , denotes ,
T14443 514-520 NNP denotes United
T14444 521-527 NNP denotes States
T14445 527-528 -RRB- denotes )
T14446 528-529 . denotes .
T14447 529-644 sentence denotes Mice carrying a null mutation of Fog2 generated by gene targeting [16] were the generous gift of Dr. Stuart Orkin.
T14448 530-534 NNS denotes Mice
T14450 535-543 VBG denotes carrying
T14451 544-545 DT denotes a
T14453 546-550 JJ denotes null
T14452 551-559 NN denotes mutation
T14454 560-562 IN denotes of
T14455 563-567 NN denotes Fog2
T14456 568-577 VBN denotes generated
T14457 578-580 IN denotes by
T14458 581-585 NN denotes gene
T14459 586-595 NN denotes targeting
T14460 596-597 -LRB- denotes [
T14461 597-599 CD denotes 16
T14462 599-600 -RRB- denotes ]
T14449 601-605 VBD denotes were
T14463 606-609 DT denotes the
T14465 610-618 JJ denotes generous
T14464 619-623 NN denotes gift
T14466 624-626 IN denotes of
T14467 627-630 NNP denotes Dr.
T14469 631-637 NNP denotes Stuart
T14468 638-643 NNP denotes Orkin
T14470 643-644 . denotes .
T15313 646-659 JJ denotes Developmental
T15314 660-668 NN denotes analysis
T15315 669-671 IN denotes of
T15316 672-676 NNS denotes mice
T15317 676-677 . denotes .
T15318 677-746 sentence denotes Timed pregnancies were set up for collection of E11.5–E17.5 embryos.
T15319 678-683 JJ denotes Timed
T15320 684-695 NNS denotes pregnancies
T15322 696-700 VBD denotes were
T15321 701-704 VBN denotes set
T15323 705-707 RP denotes up
T15324 708-711 IN denotes for
T15325 712-722 NN denotes collection
T15326 723-725 IN denotes of
T15327 726-731 NN denotes E11.5
T15329 731-732 SYM denotes –
T15330 732-737 NN denotes E17.5
T15328 738-745 NNS denotes embryos
T15331 745-746 . denotes .
T15332 746-824 sentence denotes Embryos were fixed, dehydrated, and embedded in paraffin prior to sectioning.
T15333 747-754 NNS denotes Embryos
T15335 755-759 VBD denotes were
T15334 760-765 VBN denotes fixed
T15336 765-767 , denotes ,
T15337 767-777 VBN denotes dehydrated
T15338 777-779 , denotes ,
T15339 779-782 CC denotes and
T15340 783-791 VBN denotes embedded
T15341 792-794 IN denotes in
T15342 795-803 NN denotes paraffin
T15343 804-809 JJ denotes prior
T15344 810-812 IN denotes to
T15345 813-823 NN denotes sectioning
T15346 823-824 . denotes .
T15347 824-951 sentence denotes In older embryos, a median sternotomy was performed under microscopic guidance, and diaphragm, lungs, and heart were examined.
T15348 825-827 IN denotes In
T15350 828-833 JJR denotes older
T15351 834-841 NNS denotes embryos
T15352 841-843 , denotes ,
T15353 843-844 DT denotes a
T15355 845-851 JJ denotes median
T15354 852-862 NN denotes sternotomy
T15356 863-866 VBD denotes was
T15349 867-876 VBN denotes performed
T15357 877-882 IN denotes under
T15358 883-894 JJ denotes microscopic
T15359 895-903 NN denotes guidance
T15360 903-905 , denotes ,
T15361 905-908 CC denotes and
T15362 909-918 NN denotes diaphragm
T15364 918-920 , denotes ,
T15365 920-925 NNS denotes lungs
T15366 925-927 , denotes ,
T15367 927-930 CC denotes and
T15368 931-936 NN denotes heart
T15369 937-941 VBD denotes were
T15363 942-950 VBN denotes examined
T15370 950-951 . denotes .
T15371 951-1013 sentence denotes The lungs and tracheobronchial tree were removed and weighed.
T15372 952-955 DT denotes The
T15373 956-961 NNS denotes lungs
T15375 962-965 CC denotes and
T15376 966-982 JJ denotes tracheobronchial
T15377 983-987 NN denotes tree
T15378 988-992 VBD denotes were
T15374 993-1000 VBN denotes removed
T15379 1001-1004 CC denotes and
T15380 1005-1012 VBN denotes weighed
T15381 1012-1013 . denotes .
T15382 1013-1101 sentence denotes Whole diaphragms were isolated from fixed thoracic tissue from E15.5 and E17.5 embryos.
T15383 1014-1019 JJ denotes Whole
T15384 1020-1030 NNS denotes diaphragms
T15386 1031-1035 VBD denotes were
T15385 1036-1044 VBN denotes isolated
T15387 1045-1049 IN denotes from
T15388 1050-1055 VBN denotes fixed
T15390 1056-1064 JJ denotes thoracic
T15389 1065-1071 NN denotes tissue
T15391 1072-1076 IN denotes from
T15392 1077-1082 NN denotes E15.5
T15394 1083-1086 CC denotes and
T15395 1087-1092 NN denotes E17.5
T15393 1093-1100 NNS denotes embryos
T15396 1100-1101 . denotes .
T15397 1101-1540 sentence denotes For lung explant culture, lungs were dissected from fresh embryos at E11.5 and E12.5 and placed on porous 24-mm (0.4-μ) polyester membranes floated in wells containing 2 ml of Dulbecco's modified Eagle's medium, nutrient mixture F-12 (11039–021, Gibco, San Diego, California, United States), supplemented with 10% fetal bovine serum, 0.3 mg/ml L-glutamine, 100 units/ml penicillin, 100 mcg/ml streptomycin, and 0.25 mcg/ml amphotericin B.
T15398 1102-1105 IN denotes For
T15400 1106-1110 NN denotes lung
T15402 1111-1118 NN denotes explant
T15401 1119-1126 NN denotes culture
T15403 1126-1128 , denotes ,
T15404 1128-1133 NNS denotes lungs
T15405 1134-1138 VBD denotes were
T15399 1139-1148 VBN denotes dissected
T15406 1149-1153 IN denotes from
T15407 1154-1159 JJ denotes fresh
T15408 1160-1167 NNS denotes embryos
T15409 1168-1170 IN denotes at
T15410 1171-1176 NN denotes E11.5
T15411 1177-1180 CC denotes and
T15412 1181-1186 NN denotes E12.5
T15413 1187-1190 CC denotes and
T15414 1191-1197 VBN denotes placed
T15415 1198-1200 IN denotes on
T15416 1201-1207 JJ denotes porous
T15418 1208-1210 CD denotes 24
T15420 1210-1211 HYPH denotes -
T15419 1211-1213 NN denotes mm
T15421 1214-1215 -LRB- denotes (
T15423 1215-1218 CD denotes 0.4
T15424 1218-1219 HYPH denotes -
T15422 1219-1220 NN denotes μ
T15425 1220-1221 -RRB- denotes )
T15426 1222-1231 NN denotes polyester
T15417 1232-1241 NNS denotes membranes
T15427 1242-1249 VBN denotes floated
T15428 1250-1252 IN denotes in
T15429 1253-1258 NNS denotes wells
T15430 1259-1269 VBG denotes containing
T15431 1270-1271 CD denotes 2
T15432 1272-1274 NN denotes ml
T15433 1275-1277 IN denotes of
T15434 1278-1286 NNP denotes Dulbecco
T15436 1286-1288 POS denotes 's
T15437 1289-1297 VBN denotes modified
T15438 1298-1303 NNP denotes Eagle
T15439 1303-1305 POS denotes 's
T15435 1306-1312 NN denotes medium
T15440 1312-1314 , denotes ,
T15441 1314-1322 NN denotes nutrient
T15442 1323-1330 NN denotes mixture
T15443 1331-1332 NN denotes F
T15444 1332-1333 HYPH denotes -
T15445 1333-1335 CD denotes 12
T15446 1336-1337 -LRB- denotes (
T15448 1337-1342 CD denotes 11039
T15450 1342-1343 HYPH denotes –
T15449 1343-1346 CD denotes 021
T15451 1346-1348 , denotes ,
T15447 1348-1353 NNP denotes Gibco
T15452 1353-1355 , denotes ,
T15453 1355-1358 NNP denotes San
T15454 1359-1364 NNP denotes Diego
T15455 1364-1366 , denotes ,
T15456 1366-1376 NNP denotes California
T15457 1376-1378 , denotes ,
T15458 1378-1384 NNP denotes United
T15459 1385-1391 NNP denotes States
T15460 1391-1392 -RRB- denotes )
T15461 1392-1394 , denotes ,
T15462 1394-1406 VBN denotes supplemented
T15463 1407-1411 IN denotes with
T15464 1412-1414 CD denotes 10
T15465 1414-1415 NN denotes %
T15467 1416-1421 JJ denotes fetal
T15468 1422-1428 JJ denotes bovine
T15466 1429-1434 NN denotes serum
T15469 1434-1436 , denotes ,
T15470 1436-1439 CD denotes 0.3
T15471 1440-1442 NN denotes mg
T15473 1442-1443 SYM denotes /
T15474 1443-1445 NN denotes ml
T15475 1446-1447 NN denotes L
T15476 1447-1448 HYPH denotes -
T15472 1448-1457 NN denotes glutamine
T15477 1457-1459 , denotes ,
T15478 1459-1462 CD denotes 100
T15479 1463-1468 NNS denotes units
T15481 1468-1469 SYM denotes /
T15482 1469-1471 NN denotes ml
T15480 1472-1482 NN denotes penicillin
T15483 1482-1484 , denotes ,
T15484 1484-1487 CD denotes 100
T15485 1488-1491 NN denotes mcg
T15487 1491-1492 SYM denotes /
T15488 1492-1494 NN denotes ml
T15486 1495-1507 NN denotes streptomycin
T15489 1507-1509 , denotes ,
T15490 1509-1512 CC denotes and
T15491 1513-1517 CD denotes 0.25
T15492 1518-1521 NN denotes mcg
T15494 1521-1522 SYM denotes /
T15495 1522-1524 NN denotes ml
T15496 1525-1537 NN denotes amphotericin
T15493 1538-1539 NN denotes B
T15497 1539-1540 . denotes .
T15498 1540-1610 sentence denotes Lung explants were cultured at 37 °C in 95% air/5% CO2 for up to 5 d.
T15499 1541-1545 NN denotes Lung
T15500 1546-1554 NNS denotes explants
T15502 1555-1559 VBD denotes were
T15501 1560-1568 VBN denotes cultured
T15503 1569-1571 IN denotes at
T15504 1572-1574 CD denotes 37
T15505 1575-1577 NN denotes °C
T15506 1578-1580 IN denotes in
T15507 1581-1583 CD denotes 95
T15508 1583-1584 NN denotes %
T15509 1585-1588 NN denotes air
T15510 1588-1589 HYPH denotes /
T15511 1589-1590 CD denotes 5
T15512 1590-1591 NN denotes %
T15513 1592-1595 NN denotes CO2
T15514 1596-1599 IN denotes for
T15515 1600-1602 RB denotes up
T15517 1603-1605 IN denotes to
T15516 1606-1607 CD denotes 5
T15518 1608-1609 NN denotes d
T15519 1609-1610 . denotes .
T15520 1610-1746 sentence denotes They were photographed daily with a dissecting microscope (MZ12.5, Leica, Wetzlar, Germany) equipped with a Leica DC500 digital camera.
T15521 1611-1615 PRP denotes They
T15523 1616-1620 VBD denotes were
T15522 1621-1633 VBN denotes photographed
T15524 1634-1639 RB denotes daily
T15525 1640-1644 IN denotes with
T15526 1645-1646 DT denotes a
T15528 1647-1657 VBG denotes dissecting
T15527 1658-1668 NN denotes microscope
T15529 1669-1670 -LRB- denotes (
T15530 1670-1676 NN denotes MZ12.5
T15531 1676-1678 , denotes ,
T15532 1678-1683 NNP denotes Leica
T15533 1683-1685 , denotes ,
T15534 1685-1692 NNP denotes Wetzlar
T15535 1692-1694 , denotes ,
T15536 1694-1701 NNP denotes Germany
T15537 1701-1702 -RRB- denotes )
T15538 1703-1711 VBN denotes equipped
T15539 1712-1716 IN denotes with
T15540 1717-1718 DT denotes a
T15542 1719-1724 NNP denotes Leica
T15543 1725-1730 NN denotes DC500
T15544 1731-1738 JJ denotes digital
T15541 1739-1745 NN denotes camera
T15545 1745-1746 . denotes .
T15546 1746-1849 sentence denotes Transgenic mice carrying the lacZ gene driven by the Fog2 promoter have been developed by S. Tevosian.
T15547 1747-1757 JJ denotes Transgenic
T15548 1758-1762 NNS denotes mice
T15550 1763-1771 VBG denotes carrying
T15551 1772-1775 DT denotes the
T15553 1776-1780 NN denotes lacZ
T15552 1781-1785 NN denotes gene
T15554 1786-1792 VBN denotes driven
T15555 1793-1795 IN denotes by
T15556 1796-1799 DT denotes the
T15558 1800-1804 NN denotes Fog2
T15557 1805-1813 NN denotes promoter
T15559 1814-1818 VBP denotes have
T15560 1819-1823 VBN denotes been
T15549 1824-1833 VBN denotes developed
T15561 1834-1836 IN denotes by
T15562 1837-1839 NNP denotes S.
T15563 1840-1848 NNP denotes Tevosian
T15564 1848-1849 . denotes .
T15565 1849-2049 sentence denotes In these animals, the lacZ gene is incorporated (“knocked-in”) into the Fog2 locus to allow β-galactosidase expression as a fusion protein in frame with the first 235 amino acids of the FOG2 protein.
T15566 1850-1852 IN denotes In
T15568 1853-1858 DT denotes these
T15569 1859-1866 NNS denotes animals
T15570 1866-1868 , denotes ,
T15571 1868-1871 DT denotes the
T15573 1872-1876 NN denotes lacZ
T15572 1877-1881 NN denotes gene
T15574 1882-1884 VBZ denotes is
T15567 1885-1897 VBN denotes incorporated
T15575 1898-1899 -LRB- denotes (
T15577 1899-1900 `` denotes “
T15576 1900-1907 VBN denotes knocked
T15578 1907-1908 HYPH denotes -
T15579 1908-1910 RP denotes in
T15580 1910-1911 '' denotes ”
T15581 1911-1912 -RRB- denotes )
T15582 1913-1917 IN denotes into
T15583 1918-1921 DT denotes the
T15585 1922-1926 NN denotes Fog2
T15584 1927-1932 NN denotes locus
T15586 1933-1935 TO denotes to
T15587 1936-1941 VB denotes allow
T15588 1942-1943 NN denotes β
T15590 1943-1944 HYPH denotes -
T15589 1944-1957 NN denotes galactosidase
T15591 1958-1968 NN denotes expression
T15592 1969-1971 IN denotes as
T15593 1972-1973 DT denotes a
T15595 1974-1980 NN denotes fusion
T15594 1981-1988 NN denotes protein
T15596 1989-1991 IN denotes in
T15597 1992-1997 NN denotes frame
T15598 1998-2002 IN denotes with
T15599 2003-2006 DT denotes the
T15601 2007-2012 JJ denotes first
T15602 2013-2016 CD denotes 235
T15603 2017-2022 NN denotes amino
T15600 2023-2028 NNS denotes acids
T15604 2029-2031 IN denotes of
T15605 2032-2035 DT denotes the
T15607 2036-2040 NN denotes FOG2
T15606 2041-2048 NN denotes protein
T15608 2048-2049 . denotes .
T15609 2049-2108 sentence denotes The Fog2-lacZ module is followed by an ires-eGFP cassette.
T15610 2050-2053 DT denotes The
T15612 2054-2058 NN denotes Fog2
T15614 2058-2059 HYPH denotes -
T15613 2059-2063 NN denotes lacZ
T15611 2064-2070 NN denotes module
T15616 2071-2073 VBZ denotes is
T15615 2074-2082 VBN denotes followed
T15617 2083-2085 IN denotes by
T15618 2086-2088 DT denotes an
T15620 2089-2093 NN denotes ires
T15622 2093-2094 HYPH denotes -
T15621 2094-2098 NN denotes eGFP
T15619 2099-2107 NN denotes cassette
T15623 2107-2108 . denotes .
T15624 2108-2149 sentence denotes This creates a null allele of Fog2 gene.
T15625 2109-2113 DT denotes This
T15626 2114-2121 VBZ denotes creates
T15627 2122-2123 DT denotes a
T15629 2124-2128 JJ denotes null
T15628 2129-2135 NN denotes allele
T15630 2136-2138 IN denotes of
T15631 2139-2143 NN denotes Fog2
T15632 2144-2148 NN denotes gene
T15633 2148-2149 . denotes .
T15634 2149-2245 sentence denotes The Fog2-LacZ-eGFP construct was linearized with KspI and electroporated into the CJ7 ES cells.
T15635 2150-2153 DT denotes The
T15637 2154-2158 NN denotes Fog2
T15639 2158-2159 HYPH denotes -
T15640 2159-2163 NN denotes LacZ
T15641 2163-2164 HYPH denotes -
T15638 2164-2168 NN denotes eGFP
T15636 2169-2178 NN denotes construct
T15643 2179-2182 VBD denotes was
T15642 2183-2193 VBN denotes linearized
T15644 2194-2198 IN denotes with
T15645 2199-2203 NN denotes KspI
T15646 2204-2207 CC denotes and
T15647 2208-2222 VBN denotes electroporated
T15648 2223-2227 IN denotes into
T15649 2228-2231 DT denotes the
T15651 2232-2235 NN denotes CJ7
T15652 2236-2238 NN denotes ES
T15650 2239-2244 NNS denotes cells
T15653 2244-2245 . denotes .
T15654 2245-2357 sentence denotes The correctly targeted clone was selected by the Southern blot analysis and injected into C57BL/6J blastocysts.
T15655 2246-2249 DT denotes The
T15657 2250-2259 RB denotes correctly
T15658 2260-2268 VBN denotes targeted
T15656 2269-2274 NN denotes clone
T15660 2275-2278 VBD denotes was
T15659 2279-2287 VBN denotes selected
T15661 2288-2290 IN denotes by
T15662 2291-2294 DT denotes the
T15664 2295-2303 NNP denotes Southern
T15665 2304-2308 NN denotes blot
T15663 2309-2317 NN denotes analysis
T15666 2318-2321 CC denotes and
T15667 2322-2330 VBN denotes injected
T15668 2331-2335 IN denotes into
T15669 2336-2341 NN denotes C57BL
T15671 2341-2342 HYPH denotes /
T15670 2342-2344 NN denotes 6J
T15672 2345-2356 NNS denotes blastocysts
T15673 2356-2357 . denotes .
T15674 2357-2434 sentence denotes Fog2-lacZ-eGFP animals were maintained on the mixed C57BL/6J/129 background.
T15675 2358-2362 NN denotes Fog2
T15677 2362-2363 HYPH denotes -
T15678 2363-2367 NN denotes lacZ
T15679 2367-2368 HYPH denotes -
T15676 2368-2372 NN denotes eGFP
T15680 2373-2380 NNS denotes animals
T15682 2381-2385 VBD denotes were
T15681 2386-2396 VBN denotes maintained
T15683 2397-2399 IN denotes on
T15684 2400-2403 DT denotes the
T15686 2404-2409 JJ denotes mixed
T15687 2410-2415 NN denotes C57BL
T15689 2415-2416 HYPH denotes /
T15688 2416-2418 NN denotes 6J
T15690 2418-2419 HYPH denotes /
T15691 2419-2422 CD denotes 129
T15685 2423-2433 NN denotes background
T15692 2433-2434 . denotes .
T15693 2434-2577 sentence denotes lacZ Expression in whole dissected embryonic lungs was analyzed by staining for β-galactosidase activity with X-gal after fixation for 30 min.
T15694 2435-2439 NN denotes lacZ
T15695 2440-2450 NN denotes Expression
T15697 2451-2453 IN denotes in
T15698 2454-2459 JJ denotes whole
T15700 2460-2469 VBN denotes dissected
T15701 2470-2479 JJ denotes embryonic
T15699 2480-2485 NNS denotes lungs
T15702 2486-2489 VBD denotes was
T15696 2490-2498 VBN denotes analyzed
T15703 2499-2501 IN denotes by
T15704 2502-2510 VBG denotes staining
T15705 2511-2514 IN denotes for
T15706 2515-2516 NN denotes β
T15708 2516-2517 HYPH denotes -
T15707 2517-2530 NN denotes galactosidase
T15709 2531-2539 NN denotes activity
T15710 2540-2544 IN denotes with
T15711 2545-2546 NN denotes X
T15713 2546-2547 HYPH denotes -
T15712 2547-2550 NN denotes gal
T15714 2551-2556 IN denotes after
T15715 2557-2565 NN denotes fixation
T15716 2566-2569 IN denotes for
T15717 2570-2572 CD denotes 30
T15718 2573-2576 NN denotes min
T15719 2576-2577 . denotes .
T16000 2579-2581 NN denotes RT
T16002 2581-2582 HYPH denotes -
T16001 2582-2585 NN denotes PCR
T16003 2586-2589 CC denotes and
T16004 2590-2598 NN denotes sequence
T16005 2599-2607 NN denotes analysis
T16006 2608-2610 IN denotes in
T16007 2611-2614 DT denotes the
T16008 2615-2620 NN denotes mouse
T16009 2620-2621 . denotes .
T16010 2621-2694 sentence denotes RNA was extracted by standard techniques from thoracic embryonic tissue.
T16011 2622-2625 NN denotes RNA
T16013 2626-2629 VBD denotes was
T16012 2630-2639 VBN denotes extracted
T16014 2640-2642 IN denotes by
T16015 2643-2651 JJ denotes standard
T16016 2652-2662 NNS denotes techniques
T16017 2663-2667 IN denotes from
T16018 2668-2676 JJ denotes thoracic
T16020 2677-2686 JJ denotes embryonic
T16019 2687-2693 NN denotes tissue
T16021 2693-2694 . denotes .
T16022 2694-2770 sentence denotes RT-PCR was performed using six primer sets designed to cover the Fog2 gene.
T16023 2695-2697 NN denotes RT
T16025 2697-2698 HYPH denotes -
T16024 2698-2701 NN denotes PCR
T16027 2702-2705 VBD denotes was
T16026 2706-2715 VBN denotes performed
T16028 2716-2721 VBG denotes using
T16029 2722-2725 CD denotes six
T16031 2726-2732 NN denotes primer
T16030 2733-2737 NNS denotes sets
T16032 2738-2746 VBN denotes designed
T16033 2747-2749 TO denotes to
T16034 2750-2755 VB denotes cover
T16035 2756-2759 DT denotes the
T16037 2760-2764 NN denotes Fog2
T16036 2765-2769 NN denotes gene
T16038 2769-2770 . denotes .
T16039 2770-2969 sentence denotes RT-PCR was repeated with radiolabeled primers to amplify an abnormally spliced region of the gene (Table S1), and the product was run on a denaturing sequencing gel according to standard techniques.
T16040 2771-2773 NN denotes RT
T16042 2773-2774 HYPH denotes -
T16041 2774-2777 NN denotes PCR
T16044 2778-2781 VBD denotes was
T16043 2782-2790 VBN denotes repeated
T16045 2791-2795 IN denotes with
T16046 2796-2808 VBN denotes radiolabeled
T16047 2809-2816 NNS denotes primers
T16048 2817-2819 TO denotes to
T16049 2820-2827 VB denotes amplify
T16050 2828-2830 DT denotes an
T16052 2831-2841 RB denotes abnormally
T16053 2842-2849 VBN denotes spliced
T16051 2850-2856 NN denotes region
T16054 2857-2859 IN denotes of
T16055 2860-2863 DT denotes the
T16056 2864-2868 NN denotes gene
T16057 2869-2870 -LRB- denotes (
T16059 2870-2875 NN denotes Table
T16058 2876-2878 NN denotes S1
T16060 2878-2879 -RRB- denotes )
T16061 2879-2881 , denotes ,
T16062 2881-2884 CC denotes and
T16063 2885-2888 DT denotes the
T16064 2889-2896 NN denotes product
T16066 2897-2900 VBD denotes was
T16065 2901-2904 VBN denotes run
T16067 2905-2907 IN denotes on
T16068 2908-2909 DT denotes a
T16070 2910-2920 VBG denotes denaturing
T16071 2921-2931 NN denotes sequencing
T16069 2932-2935 NN denotes gel
T16072 2936-2945 VBG denotes according
T16073 2946-2948 IN denotes to
T16074 2949-2957 JJ denotes standard
T16075 2958-2968 NNS denotes techniques
T16076 2968-2969 . denotes .
T16077 2969-3137 sentence denotes The RT-PCR product was cloned into pCR2.1 vector using TOPO TA Cloning Kit (Invitrogen, Carlsbad, California, United States) and sequenced using gene-specific primers.
T16078 2970-2973 DT denotes The
T16080 2974-2976 NN denotes RT
T16082 2976-2977 HYPH denotes -
T16081 2977-2980 NN denotes PCR
T16079 2981-2988 NN denotes product
T16084 2989-2992 VBD denotes was
T16083 2993-2999 VBN denotes cloned
T16085 3000-3004 IN denotes into
T16086 3005-3011 NN denotes pCR2.1
T16087 3012-3018 NN denotes vector
T16088 3019-3024 VBG denotes using
T16089 3025-3029 NN denotes TOPO
T16091 3030-3032 NN denotes TA
T16092 3033-3040 NNP denotes Cloning
T16090 3041-3044 NNP denotes Kit
T16093 3045-3046 -LRB- denotes (
T16094 3046-3056 NNP denotes Invitrogen
T16095 3056-3058 , denotes ,
T16096 3058-3066 NNP denotes Carlsbad
T16097 3066-3068 , denotes ,
T16098 3068-3078 NNP denotes California
T16099 3078-3080 , denotes ,
T16100 3080-3086 NNP denotes United
T16101 3087-3093 NNP denotes States
T16102 3093-3094 -RRB- denotes )
T16103 3095-3098 CC denotes and
T16104 3099-3108 VBN denotes sequenced
T16105 3109-3114 VBG denotes using
T16106 3115-3119 NN denotes gene
T16108 3119-3120 HYPH denotes -
T16107 3120-3128 JJ denotes specific
T16109 3129-3136 NNS denotes primers
T16110 3136-3137 . denotes .
T16111 3137-3235 sentence denotes Sequence analysis was done using Sequencher 4.1 (Gene Codes, Ann Arbor, Michigan, United States).
T16112 3138-3146 NN denotes Sequence
T16113 3147-3155 NN denotes analysis
T16115 3156-3159 VBD denotes was
T16114 3160-3164 VBN denotes done
T16116 3165-3170 VBG denotes using
T16117 3171-3181 NNP denotes Sequencher
T16118 3182-3185 CD denotes 4.1
T16119 3186-3187 -LRB- denotes (
T16121 3187-3191 NNP denotes Gene
T16120 3192-3197 NNPS denotes Codes
T16122 3197-3199 , denotes ,
T16123 3199-3202 NNP denotes Ann
T16124 3203-3208 NNP denotes Arbor
T16125 3208-3210 , denotes ,
T16126 3210-3218 NNP denotes Michigan
T16127 3218-3220 , denotes ,
T16128 3220-3226 NNP denotes United
T16129 3227-3233 NNP denotes States
T16130 3233-3234 -RRB- denotes )
T16131 3234-3235 . denotes .
T16289 3237-3239 FW denotes In
T16290 3240-3244 FW denotes situ
T16291 3245-3258 NN denotes hybridization
T16292 3258-3259 . denotes .
T16293 3259-3393 sentence denotes After dehydration and embedding in paraffin wax, 10-μ sections were subjected to radioactive in situ hybridization as described [53].
T16294 3260-3265 IN denotes After
T16296 3266-3277 NN denotes dehydration
T16297 3278-3281 CC denotes and
T16298 3282-3291 NN denotes embedding
T16299 3292-3294 IN denotes in
T16300 3295-3303 NN denotes paraffin
T16301 3304-3307 NN denotes wax
T16302 3307-3309 , denotes ,
T16303 3309-3311 CD denotes 10
T16305 3311-3312 HYPH denotes -
T16304 3312-3313 NN denotes μ
T16306 3314-3322 NNS denotes sections
T16307 3323-3327 VBD denotes were
T16295 3328-3337 VBN denotes subjected
T16308 3338-3340 IN denotes to
T16309 3341-3352 JJ denotes radioactive
T16311 3353-3355 FW denotes in
T16312 3356-3360 FW denotes situ
T16310 3361-3374 NN denotes hybridization
T16313 3375-3377 IN denotes as
T16314 3378-3387 VBN denotes described
T16315 3388-3389 -LRB- denotes [
T16316 3389-3391 CD denotes 53
T16317 3391-3392 -RRB- denotes ]
T16318 3392-3393 . denotes .
T16319 3393-3523 sentence denotes Probes labeled with 35S were prepared by run-off transcription of linearized plasmid templates and hybridized to tissue sections.
T16320 3394-3400 NNS denotes Probes
T16322 3401-3408 VBN denotes labeled
T16323 3409-3413 IN denotes with
T16324 3414-3417 NN denotes 35S
T16325 3418-3422 VBD denotes were
T16321 3423-3431 VBN denotes prepared
T16326 3432-3434 IN denotes by
T16327 3435-3438 VB denotes run
T16329 3438-3439 HYPH denotes -
T16330 3439-3442 RP denotes off
T16328 3443-3456 NN denotes transcription
T16331 3457-3459 IN denotes of
T16332 3460-3470 VBN denotes linearized
T16334 3471-3478 NN denotes plasmid
T16333 3479-3488 NNS denotes templates
T16335 3489-3492 CC denotes and
T16336 3493-3503 VBN denotes hybridized
T16337 3504-3506 IN denotes to
T16338 3507-3513 NN denotes tissue
T16339 3514-3522 NNS denotes sections
T16340 3522-3523 . denotes .
T16341 3523-3636 sentence denotes Nuclei were counterstained with Hoescht 33258, and signal was imaged using fluorescent and darkfield microscopy.
T16342 3524-3530 NNS denotes Nuclei
T16344 3531-3535 VBD denotes were
T16343 3536-3550 VBN denotes counterstained
T16345 3551-3555 IN denotes with
T16346 3556-3563 NNP denotes Hoescht
T16347 3564-3569 CD denotes 33258
T16348 3569-3571 , denotes ,
T16349 3571-3574 CC denotes and
T16350 3575-3581 NN denotes signal
T16352 3582-3585 VBD denotes was
T16351 3586-3592 VBN denotes imaged
T16353 3593-3598 VBG denotes using
T16354 3599-3610 JJ denotes fluorescent
T16356 3611-3614 CC denotes and
T16357 3615-3624 NN denotes darkfield
T16355 3625-3635 NN denotes microscopy
T16358 3635-3636 . denotes .
T16688 3638-3643 JJ denotes Human
T16690 3644-3647 NN denotes DNA
T16689 3648-3658 NN denotes extraction
T16691 3659-3662 CC denotes and
T16692 3663-3671 NN denotes sequence
T16693 3672-3680 NN denotes analysis
T16694 3680-3681 . denotes .
T16695 3681-3808 sentence denotes DNA was isolated from paraffin blocks by phenol-chloroform extraction [54,55], and from frozen tissues by standard techniques.
T16696 3682-3685 NN denotes DNA
T16698 3686-3689 VBD denotes was
T16697 3690-3698 VBN denotes isolated
T16699 3699-3703 IN denotes from
T16700 3704-3712 NN denotes paraffin
T16701 3713-3719 NNS denotes blocks
T16702 3720-3722 IN denotes by
T16703 3723-3729 NN denotes phenol
T16705 3729-3730 HYPH denotes -
T16704 3730-3740 NN denotes chloroform
T16706 3741-3751 NN denotes extraction
T16707 3752-3753 -LRB- denotes [
T16709 3753-3755 CD denotes 54
T16710 3755-3756 , denotes ,
T16708 3756-3758 CD denotes 55
T16711 3758-3759 -RRB- denotes ]
T16712 3759-3761 , denotes ,
T16713 3761-3764 CC denotes and
T16714 3765-3769 IN denotes from
T16715 3770-3776 VBN denotes frozen
T16716 3777-3784 NNS denotes tissues
T16717 3785-3787 IN denotes by
T16718 3788-3796 JJ denotes standard
T16719 3797-3807 NNS denotes techniques
T16720 3807-3808 . denotes .
T16721 3808-3916 sentence denotes Primers were designed to amplify FOG2 coding exons plus 50 bp of flanking upstream and downstream sequence.
T16722 3809-3816 NNS denotes Primers
T16724 3817-3821 VBD denotes were
T16723 3822-3830 VBN denotes designed
T16725 3831-3833 TO denotes to
T16726 3834-3841 VB denotes amplify
T16727 3842-3846 NN denotes FOG2
T16729 3847-3853 NN denotes coding
T16728 3854-3859 NNS denotes exons
T16730 3860-3864 CC denotes plus
T16731 3865-3867 CD denotes 50
T16732 3868-3870 NN denotes bp
T16733 3871-3873 IN denotes of
T16734 3874-3882 VBG denotes flanking
T16736 3883-3891 JJ denotes upstream
T16737 3892-3895 CC denotes and
T16738 3896-3906 JJ denotes downstream
T16735 3907-3915 NN denotes sequence
T16739 3915-3916 . denotes .
T16740 3916-3985 sentence denotes PCR amplification and sequencing were performed by standard methods.
T16741 3917-3920 NN denotes PCR
T16742 3921-3934 NN denotes amplification
T16744 3935-3938 CC denotes and
T16745 3939-3949 NN denotes sequencing
T16746 3950-3954 VBD denotes were
T16743 3955-3964 VBN denotes performed
T16747 3965-3967 IN denotes by
T16748 3968-3976 JJ denotes standard
T16749 3977-3984 NNS denotes methods
T16750 3984-3985 . denotes .
T16751 3985-4031 sentence denotes Primer sequences used are listed in Table S2.
T16752 3986-3992 NN denotes Primer
T16753 3993-4002 NNS denotes sequences
T16755 4003-4007 VBN denotes used
T16756 4008-4011 VBP denotes are
T16754 4012-4018 VBN denotes listed
T16757 4019-4021 IN denotes in
T16758 4022-4027 NN denotes Table
T16759 4028-4030 NN denotes S2
T16760 4030-4031 . denotes .
T16761 4031-4092 sentence denotes Sequence analysis was done with Sequencher 4.1 (Gene Codes).
T16762 4032-4040 NN denotes Sequence
T16763 4041-4049 NN denotes analysis
T16765 4050-4053 VBD denotes was
T16764 4054-4058 VBN denotes done
T16766 4059-4063 IN denotes with
T16767 4064-4074 NNP denotes Sequencher
T16768 4075-4078 CD denotes 4.1
T16769 4079-4080 -LRB- denotes (
T16771 4080-4084 NNP denotes Gene
T16770 4085-4090 NNPS denotes Codes
T16772 4090-4091 -RRB- denotes )
T16773 4091-4092 . denotes .
T16774 4092-4176 sentence denotes DNA from the parents of one autopsy patient was extracted from fresh blood samples.
T16775 4093-4096 NN denotes DNA
T16777 4097-4101 IN denotes from
T16778 4102-4105 DT denotes the
T16779 4106-4113 NNS denotes parents
T16780 4114-4116 IN denotes of
T16781 4117-4120 CD denotes one
T16783 4121-4128 NN denotes autopsy
T16782 4129-4136 NN denotes patient
T16784 4137-4140 VBD denotes was
T16776 4141-4150 VBN denotes extracted
T16785 4151-4155 IN denotes from
T16786 4156-4161 JJ denotes fresh
T16788 4162-4167 NN denotes blood
T16787 4168-4175 NNS denotes samples
T16789 4175-4176 . denotes .
T16790 4176-4306 sentence denotes A second set of blood samples was sent to an outside CLIA-certified laboratory for DNA extraction, PCR, sequencing, and analysis.
T16791 4177-4178 DT denotes A
T16793 4179-4185 JJ denotes second
T16792 4186-4189 NN denotes set
T16795 4190-4192 IN denotes of
T16796 4193-4198 NN denotes blood
T16797 4199-4206 NNS denotes samples
T16798 4207-4210 VBD denotes was
T16794 4211-4215 VBN denotes sent
T16799 4216-4218 IN denotes to
T16800 4219-4221 DT denotes an
T16802 4222-4229 JJ denotes outside
T16803 4230-4234 NN denotes CLIA
T16805 4234-4235 HYPH denotes -
T16804 4235-4244 VBN denotes certified
T16801 4245-4255 NN denotes laboratory
T16806 4256-4259 IN denotes for
T16807 4260-4263 NN denotes DNA
T16808 4264-4274 NN denotes extraction
T16809 4274-4276 , denotes ,
T16810 4276-4279 NN denotes PCR
T16811 4279-4281 , denotes ,
T16812 4281-4291 NN denotes sequencing
T16813 4291-4293 , denotes ,
T16814 4293-4296 CC denotes and
T16815 4297-4305 NN denotes analysis
T16816 4305-4306 . denotes .
T16817 4306-4399 sentence denotes Paternity testing was performed by the outside laboratory using a standard panel of markers.
T16818 4307-4316 NN denotes Paternity
T16819 4317-4324 NN denotes testing
T16821 4325-4328 VBD denotes was
T16820 4329-4338 VBN denotes performed
T16822 4339-4341 IN denotes by
T16823 4342-4345 DT denotes the
T16825 4346-4353 JJ denotes outside
T16824 4354-4364 NN denotes laboratory
T16826 4365-4370 VBG denotes using
T16827 4371-4372 DT denotes a
T16829 4373-4381 JJ denotes standard
T16828 4382-4387 NN denotes panel
T16830 4388-4390 IN denotes of
T16831 4391-4398 NNS denotes markers
T16832 4398-4399 . denotes .
T16833 4399-4547 sentence denotes SNP genotyping was done using Harvard Partners Center for Genetics and Genomics genotyping core facility (Cambridge, Massachusetts, United States).
T16834 4400-4403 NN denotes SNP
T16835 4404-4414 NN denotes genotyping
T16837 4415-4418 VBD denotes was
T16836 4419-4423 VBN denotes done
T16838 4424-4429 VBG denotes using
T16839 4430-4437 NNP denotes Harvard
T16841 4438-4446 NNPS denotes Partners
T16840 4447-4453 NNP denotes Center
T16843 4454-4457 IN denotes for
T16844 4458-4466 NNP denotes Genetics
T16845 4467-4470 CC denotes and
T16846 4471-4479 NNP denotes Genomics
T16847 4480-4490 NN denotes genotyping
T16848 4491-4495 NN denotes core
T16842 4496-4504 NN denotes facility
T16849 4505-4506 -LRB- denotes (
T16850 4506-4515 NNP denotes Cambridge
T16851 4515-4517 , denotes ,
T16852 4517-4530 NNP denotes Massachusetts
T16853 4530-4532 , denotes ,
T16854 4532-4538 NNP denotes United
T16855 4539-4545 NNP denotes States
T16856 4545-4546 -RRB- denotes )
T16857 4546-4547 . denotes .
R4137 T14396 T14397 amod Genetic,mapping
R4138 T14398 T14397 prep of,mapping
R4139 T14399 T14400 det the,mutation
R4140 T14400 T14398 pobj mutation,of
R4141 T14401 T14400 compound mouse,mutation
R4142 T14402 T14400 appos lil,mutation
R4143 T14403 T14397 punct .,mapping
R4144 T14405 T14406 det The,mutation
R4145 T14406 T14408 nsubjpass mutation,identified
R4146 T14407 T14406 compound lil,mutation
R4147 T14409 T14408 auxpass was,identified
R4148 T14410 T14411 mark as,described
R4149 T14411 T14408 advcl described,identified
R4150 T14412 T14411 prep in,described
R4151 T14413 T14412 pobj results,in
R4152 T14414 T14408 punct .,identified
R4153 T14416 T14417 amod Wild,type
R4154 T14417 T14419 nmod type,mice
R4155 T14418 T14417 punct -,type
R4156 T14419 T14427 nsubjpass mice,obtained
R4157 T14420 T14421 nmod FVB,N
R4158 T14421 T14419 nmod N,mice
R4159 T14422 T14421 punct /,N
R4160 T14423 T14421 cc and,N
R4161 T14424 T14425 compound C57BL,6J
R4162 T14425 T14421 conj 6J,N
R4163 T14426 T14425 punct /,6J
R4164 T14428 T14419 acl used,mice
R4165 T14429 T14428 prep for,used
R4166 T14430 T14431 amod genetic,crosses
R4167 T14431 T14429 pobj crosses,for
R4168 T14432 T14427 auxpass were,obtained
R4169 T14433 T14427 prep from,obtained
R4170 T14434 T14435 det the,Laboratory
R4171 T14435 T14433 pobj Laboratory,from
R4172 T14436 T14435 compound Jackson,Laboratory
R4173 T14437 T14438 punct (,Harbor
R4174 T14438 T14435 parataxis Harbor,Laboratory
R4175 T14439 T14438 compound Bar,Harbor
R4176 T14440 T14438 punct ", ",Harbor
R4177 T14441 T14438 npadvmod Maine,Harbor
R4178 T14442 T14438 punct ", ",Harbor
R4179 T14443 T14444 compound United,States
R4180 T14444 T14438 npadvmod States,Harbor
R4181 T14445 T14438 punct ),Harbor
R4182 T14446 T14427 punct .,obtained
R4183 T14448 T14449 nsubj Mice,were
R4184 T14450 T14448 acl carrying,Mice
R4185 T14451 T14452 det a,mutation
R4186 T14452 T14450 dobj mutation,carrying
R4187 T14453 T14452 amod null,mutation
R4188 T14454 T14452 prep of,mutation
R4189 T14455 T14454 pobj Fog2,of
R4190 T14456 T14452 acl generated,mutation
R4191 T14457 T14456 prep by,generated
R4192 T14458 T14459 compound gene,targeting
R4193 T14459 T14457 pobj targeting,by
R4194 T14460 T14461 punct [,16
R4195 T14461 T14456 parataxis 16,generated
R4196 T14462 T14461 punct ],16
R4197 T14463 T14464 det the,gift
R4198 T14464 T14449 attr gift,were
R4199 T14465 T14464 amod generous,gift
R4200 T14466 T14464 prep of,gift
R4201 T14467 T14468 compound Dr.,Orkin
R4202 T14468 T14466 pobj Orkin,of
R4203 T14469 T14468 compound Stuart,Orkin
R4204 T14470 T14449 punct .,were
R4205 T15313 T15314 amod Developmental,analysis
R4206 T15315 T15314 prep of,analysis
R4207 T15316 T15315 pobj mice,of
R4208 T15317 T15314 punct .,analysis
R4209 T15319 T15320 amod Timed,pregnancies
R4210 T15320 T15321 nsubjpass pregnancies,set
R4211 T15322 T15321 auxpass were,set
R4212 T15323 T15321 prt up,set
R4213 T15324 T15321 prep for,set
R4214 T15325 T15324 pobj collection,for
R4215 T15326 T15325 prep of,collection
R4216 T15327 T15328 nmod E11.5,embryos
R4217 T15328 T15326 pobj embryos,of
R4218 T15329 T15330 punct –,E17.5
R4219 T15330 T15327 prep E17.5,E11.5
R4220 T15331 T15321 punct .,set
R4221 T15333 T15334 nsubj Embryos,fixed
R4222 T15335 T15334 aux were,fixed
R4223 T15336 T15334 punct ", ",fixed
R4224 T15337 T15334 conj dehydrated,fixed
R4225 T15338 T15337 punct ", ",dehydrated
R4226 T15339 T15337 cc and,dehydrated
R4227 T15340 T15337 conj embedded,dehydrated
R4228 T15341 T15340 prep in,embedded
R4229 T15342 T15341 pobj paraffin,in
R4230 T15343 T15344 amod prior,to
R4231 T15344 T15334 prep to,fixed
R4232 T15345 T15344 pobj sectioning,to
R4233 T15346 T15334 punct .,fixed
R4234 T15348 T15349 prep In,performed
R4235 T15350 T15351 amod older,embryos
R4236 T15351 T15348 pobj embryos,In
R4237 T15352 T15349 punct ", ",performed
R4238 T15353 T15354 det a,sternotomy
R4239 T15354 T15349 nsubjpass sternotomy,performed
R4240 T15355 T15354 amod median,sternotomy
R4241 T15356 T15349 auxpass was,performed
R4242 T15357 T15349 prep under,performed
R4243 T15358 T15359 amod microscopic,guidance
R4244 T15359 T15357 pobj guidance,under
R4245 T15360 T15349 punct ", ",performed
R4246 T15361 T15349 cc and,performed
R4247 T15362 T15363 nsubjpass diaphragm,examined
R4248 T15363 T15349 conj examined,performed
R4249 T15364 T15362 punct ", ",diaphragm
R4250 T15365 T15362 conj lungs,diaphragm
R4251 T15366 T15365 punct ", ",lungs
R4252 T15367 T15365 cc and,lungs
R4253 T15368 T15365 conj heart,lungs
R4254 T15369 T15363 auxpass were,examined
R4255 T15370 T15349 punct .,performed
R4256 T15372 T15373 det The,lungs
R4257 T15373 T15374 nsubjpass lungs,removed
R4258 T15375 T15373 cc and,lungs
R4259 T15376 T15377 amod tracheobronchial,tree
R4260 T15377 T15373 conj tree,lungs
R4261 T15378 T15374 auxpass were,removed
R4262 T15379 T15374 cc and,removed
R4263 T15380 T15374 conj weighed,removed
R4264 T15381 T15374 punct .,removed
R4265 T15383 T15384 amod Whole,diaphragms
R4266 T15384 T15385 nsubjpass diaphragms,isolated
R4267 T15386 T15385 auxpass were,isolated
R4268 T15387 T15385 prep from,isolated
R4269 T15388 T15389 amod fixed,tissue
R4270 T15389 T15387 pobj tissue,from
R4271 T15390 T15389 amod thoracic,tissue
R4272 T15391 T15389 prep from,tissue
R4273 T15392 T15393 nmod E15.5,embryos
R4274 T15393 T15391 pobj embryos,from
R4275 T15394 T15392 cc and,E15.5
R4276 T15395 T15392 conj E17.5,E15.5
R4277 T15396 T15385 punct .,isolated
R4278 T15398 T15399 prep For,dissected
R4279 T15400 T15401 compound lung,culture
R4280 T15401 T15398 pobj culture,For
R4281 T15402 T15401 compound explant,culture
R4282 T15403 T15399 punct ", ",dissected
R4283 T15404 T15399 nsubjpass lungs,dissected
R4284 T15405 T15399 auxpass were,dissected
R4285 T15406 T15399 prep from,dissected
R4286 T15407 T15408 amod fresh,embryos
R4287 T15408 T15406 pobj embryos,from
R4288 T15409 T15399 prep at,dissected
R4289 T15410 T15409 pobj E11.5,at
R4290 T15411 T15410 cc and,E11.5
R4291 T15412 T15410 conj E12.5,E11.5
R4292 T15413 T15399 cc and,dissected
R4293 T15414 T15399 conj placed,dissected
R4294 T15415 T15414 prep on,placed
R4295 T15416 T15417 amod porous,membranes
R4296 T15417 T15415 pobj membranes,on
R4297 T15418 T15419 nummod 24,mm
R4298 T15419 T15417 nmod mm,membranes
R4299 T15420 T15419 punct -,mm
R4300 T15421 T15422 punct (,μ
R4301 T15422 T15417 parataxis μ,membranes
R4302 T15423 T15422 nummod 0.4,μ
R4303 T15424 T15422 punct -,μ
R4304 T15425 T15422 punct ),μ
R4305 T15426 T15417 compound polyester,membranes
R4306 T15427 T15417 acl floated,membranes
R4307 T15428 T15427 prep in,floated
R4308 T15429 T15428 pobj wells,in
R4309 T15430 T15429 acl containing,wells
R4310 T15431 T15432 nummod 2,ml
R4311 T15432 T15430 dobj ml,containing
R4312 T15433 T15432 prep of,ml
R4313 T15434 T15435 poss Dulbecco,medium
R4314 T15435 T15433 pobj medium,of
R4315 T15436 T15434 case 's,Dulbecco
R4316 T15437 T15435 amod modified,medium
R4317 T15438 T15435 poss Eagle,medium
R4318 T15439 T15438 case 's,Eagle
R4319 T15440 T15435 punct ", ",medium
R4320 T15441 T15442 compound nutrient,mixture
R4321 T15442 T15443 compound mixture,F
R4322 T15443 T15435 appos F,medium
R4323 T15444 T15443 punct -,F
R4324 T15445 T15443 nummod 12,F
R4325 T15446 T15447 punct (,Gibco
R4326 T15447 T15443 parataxis Gibco,F
R4327 T15448 T15449 nummod 11039,021
R4328 T15449 T15447 dep 021,Gibco
R4329 T15450 T15449 punct –,021
R4330 T15451 T15447 punct ", ",Gibco
R4331 T15452 T15447 punct ", ",Gibco
R4332 T15453 T15454 compound San,Diego
R4333 T15454 T15447 npadvmod Diego,Gibco
R4334 T15455 T15447 punct ", ",Gibco
R4335 T15456 T15447 npadvmod California,Gibco
R4336 T15457 T15447 punct ", ",Gibco
R4337 T15458 T15459 compound United,States
R4338 T15459 T15447 npadvmod States,Gibco
R4339 T15460 T15447 punct ),Gibco
R4340 T15461 T15435 punct ", ",medium
R4341 T15462 T15435 acl supplemented,medium
R4342 T15463 T15462 prep with,supplemented
R4343 T15464 T15465 nummod 10,%
R4344 T15465 T15466 nmod %,serum
R4345 T15466 T15463 pobj serum,with
R4346 T15467 T15466 amod fetal,serum
R4347 T15468 T15466 amod bovine,serum
R4348 T15469 T15466 punct ", ",serum
R4349 T15470 T15471 nummod 0.3,mg
R4350 T15471 T15472 nmod mg,glutamine
R4351 T15472 T15466 conj glutamine,serum
R4352 T15473 T15474 punct /,ml
R4353 T15474 T15471 prep ml,mg
R4354 T15475 T15472 compound L,glutamine
R4355 T15476 T15472 punct -,glutamine
R4356 T15477 T15472 punct ", ",glutamine
R4357 T15478 T15479 nummod 100,units
R4358 T15479 T15480 nmod units,penicillin
R4359 T15480 T15472 conj penicillin,glutamine
R4360 T15481 T15482 punct /,ml
R4361 T15482 T15479 prep ml,units
R4362 T15483 T15480 punct ", ",penicillin
R4363 T15484 T15485 nummod 100,mcg
R4364 T15485 T15486 nmod mcg,streptomycin
R4365 T15486 T15480 conj streptomycin,penicillin
R4366 T15487 T15488 punct /,ml
R4367 T15488 T15485 prep ml,mcg
R4368 T15489 T15486 punct ", ",streptomycin
R4369 T15490 T15486 cc and,streptomycin
R4370 T15491 T15492 nummod 0.25,mcg
R4371 T15492 T15493 nmod mcg,B
R4372 T15493 T15486 conj B,streptomycin
R4373 T15494 T15495 punct /,ml
R4374 T15495 T15492 prep ml,mcg
R4375 T15496 T15493 compound amphotericin,B
R4376 T15497 T15399 punct .,dissected
R4377 T15499 T15500 compound Lung,explants
R4378 T15500 T15501 nsubjpass explants,cultured
R4379 T15502 T15501 auxpass were,cultured
R4380 T15503 T15501 prep at,cultured
R4381 T15504 T15505 nummod 37,°C
R4382 T15505 T15503 pobj °C,at
R4383 T15506 T15501 prep in,cultured
R4384 T15507 T15508 nummod 95,%
R4385 T15508 T15509 compound %,air
R4386 T15509 T15506 pobj air,in
R4387 T15510 T15509 punct /,air
R4388 T15511 T15512 nummod 5,%
R4389 T15512 T15513 compound %,CO2
R4390 T15513 T15509 appos CO2,air
R4391 T15514 T15501 prep for,cultured
R4392 T15515 T15516 advmod up,5
R4393 T15516 T15518 nummod 5,d
R4394 T15517 T15516 quantmod to,5
R4395 T15518 T15514 pobj d,for
R4396 T15519 T15501 punct .,cultured
R4397 T15521 T15522 nsubjpass They,photographed
R4398 T15523 T15522 auxpass were,photographed
R4399 T15524 T15522 advmod daily,photographed
R4400 T15525 T15522 prep with,photographed
R4401 T15526 T15527 det a,microscope
R4402 T15527 T15525 pobj microscope,with
R4403 T15528 T15527 amod dissecting,microscope
R4404 T15529 T15530 punct (,MZ12.5
R4405 T15530 T15527 parataxis MZ12.5,microscope
R4406 T15531 T15530 punct ", ",MZ12.5
R4407 T15532 T15530 npadvmod Leica,MZ12.5
R4408 T15533 T15530 punct ", ",MZ12.5
R4409 T15534 T15530 npadvmod Wetzlar,MZ12.5
R4410 T15535 T15530 punct ", ",MZ12.5
R4411 T15536 T15530 npadvmod Germany,MZ12.5
R4412 T15537 T15530 punct ),MZ12.5
R4413 T15538 T15527 acl equipped,microscope
R4414 T15539 T15538 prep with,equipped
R4415 T15540 T15541 det a,camera
R4416 T15541 T15539 pobj camera,with
R4417 T15542 T15543 nmod Leica,DC500
R4418 T15543 T15541 nmod DC500,camera
R4419 T15544 T15541 amod digital,camera
R4420 T15545 T15522 punct .,photographed
R4421 T15547 T15548 amod Transgenic,mice
R4422 T15548 T15549 nsubjpass mice,developed
R4423 T15550 T15548 acl carrying,mice
R4424 T15551 T15552 det the,gene
R4425 T15552 T15550 dobj gene,carrying
R4426 T15553 T15552 compound lacZ,gene
R4427 T15554 T15552 acl driven,gene
R4428 T15555 T15554 agent by,driven
R4429 T15556 T15557 det the,promoter
R4430 T15557 T15555 pobj promoter,by
R4431 T15558 T15557 compound Fog2,promoter
R4432 T15559 T15549 aux have,developed
R4433 T15560 T15549 auxpass been,developed
R4434 T15561 T15549 agent by,developed
R4435 T15562 T15563 compound S.,Tevosian
R4436 T15563 T15561 pobj Tevosian,by
R4437 T15564 T15549 punct .,developed
R4438 T15566 T15567 prep In,incorporated
R4439 T15568 T15569 det these,animals
R4440 T15569 T15566 pobj animals,In
R4441 T15570 T15567 punct ", ",incorporated
R4442 T15571 T15572 det the,gene
R4443 T15572 T15567 nsubjpass gene,incorporated
R4444 T15573 T15572 compound lacZ,gene
R4445 T15574 T15567 auxpass is,incorporated
R4446 T15575 T15576 punct (,knocked
R4447 T15576 T15567 parataxis knocked,incorporated
R4448 T15577 T15576 punct “,knocked
R4449 T15578 T15576 punct -,knocked
R4450 T15579 T15576 prt in,knocked
R4451 T15580 T15576 punct ”,knocked
R4452 T15581 T15576 punct ),knocked
R4453 T15582 T15567 prep into,incorporated
R4454 T15583 T15584 det the,locus
R4455 T15584 T15582 pobj locus,into
R4456 T15585 T15584 compound Fog2,locus
R4457 T15586 T15587 aux to,allow
R4458 T15587 T15567 advcl allow,incorporated
R4459 T15588 T15589 compound β,galactosidase
R4460 T15589 T15591 compound galactosidase,expression
R4461 T15590 T15589 punct -,galactosidase
R4462 T15591 T15587 dobj expression,allow
R4463 T15592 T15587 prep as,allow
R4464 T15593 T15594 det a,protein
R4465 T15594 T15592 pobj protein,as
R4466 T15595 T15594 compound fusion,protein
R4467 T15596 T15594 prep in,protein
R4468 T15597 T15596 pobj frame,in
R4469 T15598 T15597 prep with,frame
R4470 T15599 T15600 det the,acids
R4471 T15600 T15598 pobj acids,with
R4472 T15601 T15600 amod first,acids
R4473 T15602 T15600 nummod 235,acids
R4474 T15603 T15600 compound amino,acids
R4475 T15604 T15600 prep of,acids
R4476 T15605 T15606 det the,protein
R4477 T15606 T15604 pobj protein,of
R4478 T15607 T15606 compound FOG2,protein
R4479 T15608 T15567 punct .,incorporated
R4480 T15610 T15611 det The,module
R4481 T15611 T15615 nsubjpass module,followed
R4482 T15612 T15613 compound Fog2,lacZ
R4483 T15613 T15611 compound lacZ,module
R4484 T15614 T15613 punct -,lacZ
R4485 T15616 T15615 auxpass is,followed
R4486 T15617 T15615 agent by,followed
R4487 T15618 T15619 det an,cassette
R4488 T15619 T15617 pobj cassette,by
R4489 T15620 T15621 compound ires,eGFP
R4490 T15621 T15619 compound eGFP,cassette
R4491 T15622 T15621 punct -,eGFP
R4492 T15623 T15615 punct .,followed
R4493 T15625 T15626 nsubj This,creates
R4494 T15627 T15628 det a,allele
R4495 T15628 T15626 dobj allele,creates
R4496 T15629 T15628 amod null,allele
R4497 T15630 T15628 prep of,allele
R4498 T15631 T15632 compound Fog2,gene
R4499 T15632 T15630 pobj gene,of
R4500 T15633 T15626 punct .,creates
R4501 T15635 T15636 det The,construct
R4502 T15636 T15642 nsubjpass construct,linearized
R4503 T15637 T15638 compound Fog2,eGFP
R4504 T15638 T15636 compound eGFP,construct
R4505 T15639 T15638 punct -,eGFP
R4506 T15640 T15638 compound LacZ,eGFP
R4507 T15641 T15638 punct -,eGFP
R4508 T15643 T15642 auxpass was,linearized
R4509 T15644 T15642 prep with,linearized
R4510 T15645 T15644 pobj KspI,with
R4511 T15646 T15642 cc and,linearized
R4512 T15647 T15642 conj electroporated,linearized
R4513 T15648 T15647 prep into,electroporated
R4514 T15649 T15650 det the,cells
R4515 T15650 T15648 pobj cells,into
R4516 T15651 T15650 compound CJ7,cells
R4517 T15652 T15650 compound ES,cells
R4518 T15653 T15642 punct .,linearized
R4519 T15655 T15656 det The,clone
R4520 T15656 T15659 nsubjpass clone,selected
R4521 T15657 T15658 advmod correctly,targeted
R4522 T15658 T15656 amod targeted,clone
R4523 T15660 T15659 auxpass was,selected
R4524 T15661 T15659 prep by,selected
R4525 T15662 T15663 det the,analysis
R4526 T15663 T15661 pobj analysis,by
R4527 T15664 T15665 compound Southern,blot
R4528 T15665 T15663 compound blot,analysis
R4529 T15666 T15659 cc and,selected
R4530 T15667 T15659 conj injected,selected
R4531 T15668 T15667 prep into,injected
R4532 T15669 T15670 compound C57BL,6J
R4533 T15670 T15672 compound 6J,blastocysts
R4534 T15671 T15670 punct /,6J
R4535 T15672 T15668 pobj blastocysts,into
R4536 T15673 T15659 punct .,selected
R4537 T15675 T15676 compound Fog2,eGFP
R4538 T15676 T15680 compound eGFP,animals
R4539 T15677 T15676 punct -,eGFP
R4540 T15678 T15676 compound lacZ,eGFP
R4541 T15679 T15676 punct -,eGFP
R4542 T15680 T15681 nsubjpass animals,maintained
R4543 T15682 T15681 auxpass were,maintained
R4544 T15683 T15681 prep on,maintained
R4545 T15684 T15685 det the,background
R4546 T15685 T15683 pobj background,on
R4547 T15686 T15685 amod mixed,background
R4548 T15687 T15688 nmod C57BL,6J
R4549 T15688 T15685 nmod 6J,background
R4550 T15689 T15688 punct /,6J
R4551 T15690 T15688 punct /,6J
R4552 T15691 T15688 nummod 129,6J
R4553 T15692 T15681 punct .,maintained
R4554 T15694 T15695 compound lacZ,Expression
R4555 T15695 T15696 nsubjpass Expression,analyzed
R4556 T15697 T15695 prep in,Expression
R4557 T15698 T15699 amod whole,lungs
R4558 T15699 T15697 pobj lungs,in
R4559 T15700 T15699 amod dissected,lungs
R4560 T15701 T15699 amod embryonic,lungs
R4561 T15702 T15696 auxpass was,analyzed
R4562 T15703 T15696 prep by,analyzed
R4563 T15704 T15703 pcomp staining,by
R4564 T15705 T15704 prep for,staining
R4565 T15706 T15707 compound β,galactosidase
R4566 T15707 T15709 compound galactosidase,activity
R4567 T15708 T15707 punct -,galactosidase
R4568 T15709 T15705 pobj activity,for
R4569 T15710 T15704 prep with,staining
R4570 T15711 T15712 compound X,gal
R4571 T15712 T15710 pobj gal,with
R4572 T15713 T15712 punct -,gal
R4573 T15714 T15704 prep after,staining
R4574 T15715 T15714 pobj fixation,after
R4575 T15716 T15715 prep for,fixation
R4576 T15717 T15718 nummod 30,min
R4577 T15718 T15716 pobj min,for
R4578 T15719 T15696 punct .,analyzed
R4581 T16000 T16001 compound RT,PCR
R4582 T16002 T16001 punct -,PCR
R4583 T16003 T16001 cc and,PCR
R4584 T16004 T16005 compound sequence,analysis
R4585 T16005 T16001 conj analysis,PCR
R4586 T16006 T16001 prep in,PCR
R4587 T16007 T16008 det the,mouse
R4588 T16008 T16006 pobj mouse,in
R4589 T16009 T16001 punct .,PCR
R4590 T16011 T16012 nsubjpass RNA,extracted
R4591 T16013 T16012 auxpass was,extracted
R4592 T16014 T16012 prep by,extracted
R4593 T16015 T16016 amod standard,techniques
R4594 T16016 T16014 pobj techniques,by
R4595 T16017 T16012 prep from,extracted
R4596 T16018 T16019 amod thoracic,tissue
R4597 T16019 T16017 pobj tissue,from
R4598 T16020 T16019 amod embryonic,tissue
R4599 T16021 T16012 punct .,extracted
R4600 T16023 T16024 compound RT,PCR
R4601 T16024 T16026 nsubjpass PCR,performed
R4602 T16025 T16024 punct -,PCR
R4603 T16027 T16026 auxpass was,performed
R4604 T16028 T16026 advcl using,performed
R4605 T16029 T16030 nummod six,sets
R4606 T16030 T16028 dobj sets,using
R4607 T16031 T16030 compound primer,sets
R4608 T16032 T16030 acl designed,sets
R4609 T16033 T16034 aux to,cover
R4610 T16034 T16032 advcl cover,designed
R4611 T16035 T16036 det the,gene
R4612 T16036 T16034 dobj gene,cover
R4613 T16037 T16036 compound Fog2,gene
R4614 T16038 T16026 punct .,performed
R4615 T16040 T16041 compound RT,PCR
R4616 T16041 T16043 nsubjpass PCR,repeated
R4617 T16042 T16041 punct -,PCR
R4618 T16044 T16043 auxpass was,repeated
R4619 T16045 T16043 prep with,repeated
R4620 T16046 T16047 amod radiolabeled,primers
R4621 T16047 T16045 pobj primers,with
R4622 T16048 T16049 aux to,amplify
R4623 T16049 T16043 advcl amplify,repeated
R4624 T16050 T16051 det an,region
R4625 T16051 T16049 dobj region,amplify
R4626 T16052 T16053 advmod abnormally,spliced
R4627 T16053 T16051 amod spliced,region
R4628 T16054 T16051 prep of,region
R4629 T16055 T16056 det the,gene
R4630 T16056 T16054 pobj gene,of
R4631 T16057 T16058 punct (,S1
R4632 T16058 T16043 parataxis S1,repeated
R4633 T16059 T16058 compound Table,S1
R4634 T16060 T16058 punct ),S1
R4635 T16061 T16043 punct ", ",repeated
R4636 T16062 T16043 cc and,repeated
R4637 T16063 T16064 det the,product
R4638 T16064 T16065 nsubjpass product,run
R4639 T16065 T16043 conj run,repeated
R4640 T16066 T16065 auxpass was,run
R4641 T16067 T16065 prep on,run
R4642 T16068 T16069 det a,gel
R4643 T16069 T16067 pobj gel,on
R4644 T16070 T16069 amod denaturing,gel
R4645 T16071 T16069 compound sequencing,gel
R4646 T16072 T16065 prep according,run
R4647 T16073 T16072 prep to,according
R4648 T16074 T16075 amod standard,techniques
R4649 T16075 T16073 pobj techniques,to
R4650 T16076 T16065 punct .,run
R4651 T16078 T16079 det The,product
R4652 T16079 T16083 nsubjpass product,cloned
R4653 T16080 T16081 compound RT,PCR
R4654 T16081 T16079 compound PCR,product
R4655 T16082 T16081 punct -,PCR
R4656 T16084 T16083 auxpass was,cloned
R4657 T16085 T16083 prep into,cloned
R4658 T16086 T16087 compound pCR2.1,vector
R4659 T16087 T16085 pobj vector,into
R4660 T16088 T16083 advcl using,cloned
R4661 T16089 T16090 compound TOPO,Kit
R4662 T16090 T16088 dobj Kit,using
R4663 T16091 T16090 compound TA,Kit
R4664 T16092 T16090 compound Cloning,Kit
R4665 T16093 T16094 punct (,Invitrogen
R4666 T16094 T16090 parataxis Invitrogen,Kit
R4667 T16095 T16094 punct ", ",Invitrogen
R4668 T16096 T16094 npadvmod Carlsbad,Invitrogen
R4669 T16097 T16094 punct ", ",Invitrogen
R4670 T16098 T16094 npadvmod California,Invitrogen
R4671 T16099 T16094 punct ", ",Invitrogen
R4672 T16100 T16101 compound United,States
R4673 T16101 T16094 npadvmod States,Invitrogen
R4674 T16102 T16094 punct ),Invitrogen
R4675 T16103 T16083 cc and,cloned
R4676 T16104 T16083 conj sequenced,cloned
R4677 T16105 T16104 advcl using,sequenced
R4678 T16106 T16107 npadvmod gene,specific
R4679 T16107 T16109 amod specific,primers
R4680 T16108 T16107 punct -,specific
R4681 T16109 T16105 dobj primers,using
R4682 T16110 T16083 punct .,cloned
R4683 T16112 T16113 compound Sequence,analysis
R4684 T16113 T16114 nsubjpass analysis,done
R4685 T16115 T16114 auxpass was,done
R4686 T16116 T16114 advcl using,done
R4687 T16117 T16116 dobj Sequencher,using
R4688 T16118 T16117 nummod 4.1,Sequencher
R4689 T16119 T16120 punct (,Codes
R4690 T16120 T16117 parataxis Codes,Sequencher
R4691 T16121 T16120 compound Gene,Codes
R4692 T16122 T16120 punct ", ",Codes
R4693 T16123 T16124 compound Ann,Arbor
R4694 T16124 T16120 npadvmod Arbor,Codes
R4695 T16125 T16120 punct ", ",Codes
R4696 T16126 T16120 npadvmod Michigan,Codes
R4697 T16127 T16120 punct ", ",Codes
R4698 T16128 T16129 compound United,States
R4699 T16129 T16120 npadvmod States,Codes
R4700 T16130 T16120 punct ),Codes
R4701 T16131 T16114 punct .,done
R4702 T16289 T16290 advmod In,situ
R4703 T16290 T16291 amod situ,hybridization
R4704 T16292 T16291 punct .,hybridization
R4705 T16294 T16295 prep After,subjected
R4706 T16296 T16294 pobj dehydration,After
R4707 T16297 T16296 cc and,dehydration
R4708 T16298 T16296 conj embedding,dehydration
R4709 T16299 T16298 prep in,embedding
R4710 T16300 T16301 compound paraffin,wax
R4711 T16301 T16299 pobj wax,in
R4712 T16302 T16295 punct ", ",subjected
R4713 T16303 T16304 nummod 10,μ
R4714 T16304 T16306 compound μ,sections
R4715 T16305 T16304 punct -,μ
R4716 T16306 T16295 nsubjpass sections,subjected
R4717 T16307 T16295 auxpass were,subjected
R4718 T16308 T16295 prep to,subjected
R4719 T16309 T16310 amod radioactive,hybridization
R4720 T16310 T16308 pobj hybridization,to
R4721 T16311 T16312 advmod in,situ
R4722 T16312 T16310 amod situ,hybridization
R4723 T16313 T16314 mark as,described
R4724 T16314 T16295 advcl described,subjected
R4725 T16315 T16316 punct [,53
R4726 T16316 T16295 parataxis 53,subjected
R4727 T16317 T16316 punct ],53
R4728 T16318 T16295 punct .,subjected
R4729 T16320 T16321 nsubjpass Probes,prepared
R4730 T16322 T16320 acl labeled,Probes
R4731 T16323 T16322 prep with,labeled
R4732 T16324 T16323 pobj 35S,with
R4733 T16325 T16321 auxpass were,prepared
R4734 T16326 T16321 prep by,prepared
R4735 T16327 T16328 amod run,transcription
R4736 T16328 T16326 pobj transcription,by
R4737 T16329 T16327 punct -,run
R4738 T16330 T16327 prt off,run
R4739 T16331 T16328 prep of,transcription
R4740 T16332 T16333 amod linearized,templates
R4741 T16333 T16331 pobj templates,of
R4742 T16334 T16333 compound plasmid,templates
R4743 T16335 T16321 cc and,prepared
R4744 T16336 T16321 conj hybridized,prepared
R4745 T16337 T16336 prep to,hybridized
R4746 T16338 T16339 compound tissue,sections
R4747 T16339 T16337 pobj sections,to
R4748 T16340 T16321 punct .,prepared
R4749 T16342 T16343 nsubjpass Nuclei,counterstained
R4750 T16344 T16343 auxpass were,counterstained
R4751 T16345 T16343 prep with,counterstained
R4752 T16346 T16345 pobj Hoescht,with
R4753 T16347 T16346 nummod 33258,Hoescht
R4754 T16348 T16343 punct ", ",counterstained
R4755 T16349 T16343 cc and,counterstained
R4756 T16350 T16351 nsubjpass signal,imaged
R4757 T16351 T16343 conj imaged,counterstained
R4758 T16352 T16351 auxpass was,imaged
R4759 T16353 T16351 advcl using,imaged
R4760 T16354 T16355 amod fluorescent,microscopy
R4761 T16355 T16353 dobj microscopy,using
R4762 T16356 T16354 cc and,fluorescent
R4763 T16357 T16354 conj darkfield,fluorescent
R4764 T16358 T16351 punct .,imaged
R4765 T16688 T16689 amod Human,extraction
R4766 T16690 T16689 compound DNA,extraction
R4767 T16691 T16689 cc and,extraction
R4768 T16692 T16693 compound sequence,analysis
R4769 T16693 T16689 conj analysis,extraction
R4770 T16694 T16693 punct .,analysis
R4771 T16696 T16697 nsubjpass DNA,isolated
R4772 T16698 T16697 auxpass was,isolated
R4773 T16699 T16697 prep from,isolated
R4774 T16700 T16701 compound paraffin,blocks
R4775 T16701 T16699 pobj blocks,from
R4776 T16702 T16697 prep by,isolated
R4777 T16703 T16704 compound phenol,chloroform
R4778 T16704 T16706 compound chloroform,extraction
R4779 T16705 T16704 punct -,chloroform
R4780 T16706 T16702 pobj extraction,by
R4781 T16707 T16708 punct [,55
R4782 T16708 T16697 parataxis 55,isolated
R4783 T16709 T16708 nummod 54,55
R4784 T16710 T16708 punct ",",55
R4785 T16711 T16708 punct ],55
R4786 T16712 T16697 punct ", ",isolated
R4787 T16713 T16697 cc and,isolated
R4788 T16714 T16697 conj from,isolated
R4789 T16715 T16716 amod frozen,tissues
R4790 T16716 T16714 pobj tissues,from
R4791 T16717 T16714 prep by,from
R4792 T16718 T16719 amod standard,techniques
R4793 T16719 T16717 pobj techniques,by
R4794 T16720 T16697 punct .,isolated
R4795 T16722 T16723 nsubjpass Primers,designed
R4796 T16724 T16723 auxpass were,designed
R4797 T16725 T16726 aux to,amplify
R4798 T16726 T16723 advcl amplify,designed
R4799 T16727 T16728 compound FOG2,exons
R4800 T16728 T16726 dobj exons,amplify
R4801 T16729 T16728 compound coding,exons
R4802 T16730 T16728 cc plus,exons
R4803 T16731 T16732 nummod 50,bp
R4804 T16732 T16728 conj bp,exons
R4805 T16733 T16732 prep of,bp
R4806 T16734 T16735 amod flanking,sequence
R4807 T16735 T16733 pobj sequence,of
R4808 T16736 T16735 amod upstream,sequence
R4809 T16737 T16736 cc and,upstream
R4810 T16738 T16736 conj downstream,upstream
R4811 T16739 T16723 punct .,designed
R4812 T16741 T16742 compound PCR,amplification
R4813 T16742 T16743 nsubjpass amplification,performed
R4814 T16744 T16742 cc and,amplification
R4815 T16745 T16742 conj sequencing,amplification
R4816 T16746 T16743 auxpass were,performed
R4817 T16747 T16743 prep by,performed
R4818 T16748 T16749 amod standard,methods
R4819 T16749 T16747 pobj methods,by
R4820 T16750 T16743 punct .,performed
R4821 T16752 T16753 compound Primer,sequences
R4822 T16753 T16754 nsubjpass sequences,listed
R4823 T16755 T16753 acl used,sequences
R4824 T16756 T16754 auxpass are,listed
R4825 T16757 T16754 prep in,listed
R4826 T16758 T16759 compound Table,S2
R4827 T16759 T16757 pobj S2,in
R4828 T16760 T16754 punct .,listed
R4829 T16762 T16763 compound Sequence,analysis
R4830 T16763 T16764 nsubjpass analysis,done
R4831 T16765 T16764 auxpass was,done
R4832 T16766 T16764 prep with,done
R4833 T16767 T16766 pobj Sequencher,with
R4834 T16768 T16767 nummod 4.1,Sequencher
R4835 T16769 T16770 punct (,Codes
R4836 T16770 T16767 parataxis Codes,Sequencher
R4837 T16771 T16770 compound Gene,Codes
R4838 T16772 T16770 punct ),Codes
R4839 T16773 T16764 punct .,done
R4840 T16775 T16776 nsubjpass DNA,extracted
R4841 T16777 T16775 prep from,DNA
R4842 T16778 T16779 det the,parents
R4843 T16779 T16777 pobj parents,from
R4844 T16780 T16779 prep of,parents
R4845 T16781 T16782 nummod one,patient
R4846 T16782 T16780 pobj patient,of
R4847 T16783 T16782 compound autopsy,patient
R4848 T16784 T16776 auxpass was,extracted
R4849 T16785 T16776 prep from,extracted
R4850 T16786 T16787 amod fresh,samples
R4851 T16787 T16785 pobj samples,from
R4852 T16788 T16787 compound blood,samples
R4853 T16789 T16776 punct .,extracted
R4854 T16791 T16792 det A,set
R4855 T16792 T16794 nsubjpass set,sent
R4856 T16793 T16792 amod second,set
R4857 T16795 T16792 prep of,set
R4858 T16796 T16797 compound blood,samples
R4859 T16797 T16795 pobj samples,of
R4860 T16798 T16794 auxpass was,sent
R4861 T16799 T16794 prep to,sent
R4862 T16800 T16801 det an,laboratory
R4863 T16801 T16799 pobj laboratory,to
R4864 T16802 T16801 amod outside,laboratory
R4865 T16803 T16804 npadvmod CLIA,certified
R4866 T16804 T16801 amod certified,laboratory
R4867 T16805 T16804 punct -,certified
R4868 T16806 T16794 prep for,sent
R4869 T16807 T16808 compound DNA,extraction
R4870 T16808 T16806 pobj extraction,for
R4871 T16809 T16808 punct ", ",extraction
R4872 T16810 T16808 conj PCR,extraction
R4873 T16811 T16810 punct ", ",PCR
R4874 T16812 T16810 conj sequencing,PCR
R4875 T16813 T16812 punct ", ",sequencing
R4876 T16814 T16812 cc and,sequencing
R4877 T16815 T16812 conj analysis,sequencing
R4878 T16816 T16794 punct .,sent
R4879 T16818 T16819 compound Paternity,testing
R4880 T16819 T16820 nsubjpass testing,performed
R4881 T16821 T16820 auxpass was,performed
R4882 T16822 T16820 agent by,performed
R4883 T16823 T16824 det the,laboratory
R4884 T16824 T16822 pobj laboratory,by
R4885 T16825 T16824 amod outside,laboratory
R4886 T16826 T16820 advcl using,performed
R4887 T16827 T16828 det a,panel
R4888 T16828 T16826 dobj panel,using
R4889 T16829 T16828 amod standard,panel
R4890 T16830 T16828 prep of,panel
R4891 T16831 T16830 pobj markers,of
R4892 T16832 T16820 punct .,performed
R4893 T16834 T16835 compound SNP,genotyping
R4894 T16835 T16836 nsubjpass genotyping,done
R4895 T16837 T16836 auxpass was,done
R4896 T16838 T16836 advcl using,done
R4897 T16839 T16840 nmod Harvard,Center
R4898 T16840 T16842 nmod Center,facility
R4899 T16841 T16840 nmod Partners,Center
R4900 T16842 T16838 dobj facility,using
R4901 T16843 T16840 prep for,Center
R4902 T16844 T16843 pobj Genetics,for
R4903 T16845 T16844 cc and,Genetics
R4904 T16846 T16844 conj Genomics,Genetics
R4905 T16847 T16842 compound genotyping,facility
R4906 T16848 T16842 compound core,facility
R4907 T16849 T16850 punct (,Cambridge
R4908 T16850 T16842 parataxis Cambridge,facility
R4909 T16851 T16850 punct ", ",Cambridge
R4910 T16852 T16850 npadvmod Massachusetts,Cambridge
R4911 T16853 T16850 punct ", ",Cambridge
R4912 T16854 T16855 compound United,States
R4913 T16855 T16850 npadvmod States,Cambridge
R4914 T16856 T16850 punct ),Cambridge
R4915 T16857 T16836 punct .,done