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PMC:1181811 / 30067-31076 JSONTXT 3 Projects

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Id Subject Object Predicate Lexical cue
T6128 0-16 NNS denotes Oligonucleotides
T6130 17-21 VBD denotes were
T6129 22-27 VBN denotes based
T6131 28-30 IN denotes on
T6132 31-40 VBN denotes published
T6134 41-47 NN denotes mTas2r
T6133 48-50 NN denotes B6
T6135 51-53 CC denotes or
T6136 54-57 CD denotes 129
T6138 57-58 HYPH denotes /
T6137 58-61 NN denotes SvJ
T6140 62-66 NN denotes cDNA
T6139 67-76 NNS denotes sequences
T6141 77-79 CC denotes or
T6142 80-82 IN denotes on
T6143 83-86 DT denotes the
T6145 87-93 JJ denotes public
T6146 94-96 NN denotes B6
T6144 97-103 NN denotes genome
T6147 103-104 . denotes .
T6148 104-397 sentence denotes Entire coding regions plus ~50 kb of flanking sequence of each single-exon Tas2r was amplified from D2 or BXD RI genomic DNA (Jackson Laboratories, Bar Harbor, ME) by polymerase chain reaction (PCR) using a high-fidelity polymerase TaqPro Complete (Denville Scientific, South Plainfield, NJ).
T6149 105-111 JJ denotes Entire
T6151 112-118 NN denotes coding
T6150 119-126 NNS denotes regions
T6153 127-131 CC denotes plus
T6154 132-133 SYM denotes ~
T6155 133-135 CD denotes 50
T6156 136-138 NN denotes kb
T6157 139-141 IN denotes of
T6158 142-150 VBG denotes flanking
T6159 151-159 NN denotes sequence
T6160 160-162 IN denotes of
T6161 163-167 DT denotes each
T6163 168-174 JJ denotes single
T6165 174-175 HYPH denotes -
T6164 175-179 NN denotes exon
T6162 180-185 NN denotes Tas2r
T6166 186-189 VBD denotes was
T6152 190-199 VBN denotes amplified
T6167 200-204 IN denotes from
T6168 205-207 NN denotes D2
T6170 208-210 CC denotes or
T6171 211-214 NN denotes BXD
T6172 215-217 JJ denotes RI
T6173 218-225 JJ denotes genomic
T6169 226-229 NN denotes DNA
T6174 230-231 -LRB- denotes (
T6175 231-238 NNP denotes Jackson
T6176 239-251 NNP denotes Laboratories
T6177 251-253 , denotes ,
T6178 253-256 NNP denotes Bar
T6179 257-263 NNP denotes Harbor
T6180 263-265 , denotes ,
T6181 265-267 NNP denotes ME
T6182 267-268 -RRB- denotes )
T6183 269-271 IN denotes by
T6184 272-282 NN denotes polymerase
T6186 283-288 NN denotes chain
T6185 289-297 NN denotes reaction
T6187 298-299 -LRB- denotes (
T6188 299-302 NN denotes PCR
T6189 302-303 -RRB- denotes )
T6190 304-309 VBG denotes using
T6191 310-311 DT denotes a
T6193 312-316 JJ denotes high
T6195 316-317 HYPH denotes -
T6194 317-325 NN denotes fidelity
T6196 326-336 NN denotes polymerase
T6197 337-343 NNP denotes TaqPro
T6192 344-352 NNP denotes Complete
T6198 353-354 -LRB- denotes (
T6199 354-362 NNP denotes Denville
T6200 363-373 NNP denotes Scientific
T6201 373-375 , denotes ,
T6202 375-380 NNP denotes South
T6203 381-391 NNP denotes Plainfield
T6204 391-393 , denotes ,
T6205 393-395 NNP denotes NJ
T6206 395-396 -RRB- denotes )
T6207 396-397 . denotes .
T6208 397-544 sentence denotes PCR products were subcloned into pGemT-Easy (Promega, Madison, WI) and sequenced at the University of Maryland School of Medicine Biopolymer Core.
T6209 398-401 NN denotes PCR
T6210 402-410 NNS denotes products
T6212 411-415 VBD denotes were
T6211 416-425 VBN denotes subcloned
T6213 426-430 IN denotes into
T6214 431-436 NN denotes pGemT
T6215 436-437 HYPH denotes -
T6216 437-441 JJ denotes Easy
T6217 442-443 -LRB- denotes (
T6218 443-450 NNP denotes Promega
T6219 450-452 , denotes ,
T6220 452-459 NNP denotes Madison
T6221 459-461 , denotes ,
T6222 461-463 NNP denotes WI
T6223 463-464 -RRB- denotes )
T6224 465-468 CC denotes and
T6225 469-478 VBN denotes sequenced
T6226 479-481 IN denotes at
T6227 482-485 DT denotes the
T6229 486-496 NNP denotes University
T6230 497-499 IN denotes of
T6231 500-508 NNP denotes Maryland
T6232 509-515 NNP denotes School
T6233 516-518 IN denotes of
T6234 519-527 NNP denotes Medicine
T6235 528-538 NNP denotes Biopolymer
T6228 539-543 NNP denotes Core
T6236 543-544 . denotes .
T6237 544-679 sentence denotes The sequences of D2 products were compared to B6 sequences available in Genbank (see Additional file 2), and polymorphisms identified.
T6238 545-548 DT denotes The
T6239 549-558 NNS denotes sequences
T6241 559-561 IN denotes of
T6242 562-564 NN denotes D2
T6243 565-573 NNS denotes products
T6244 574-578 VBD denotes were
T6240 579-587 VBN denotes compared
T6245 588-590 IN denotes to
T6246 591-593 NN denotes B6
T6247 594-603 NNS denotes sequences
T6248 604-613 JJ denotes available
T6249 614-616 IN denotes in
T6250 617-624 NNP denotes Genbank
T6251 625-626 -LRB- denotes (
T6252 626-629 VB denotes see
T6253 630-640 JJ denotes Additional
T6254 641-645 NN denotes file
T6255 646-647 CD denotes 2
T6256 647-648 -RRB- denotes )
T6257 648-650 , denotes ,
T6258 650-653 CC denotes and
T6259 654-667 NNS denotes polymorphisms
T6260 668-678 VBN denotes identified
T6261 678-679 . denotes .
T6262 679-922 sentence denotes When possible, unique restriction sites were identified that differentiated B6 and D2 alleles, and the corresponding restriction endonucleases were used in diagnostic digests of Tas2r cDNAs amplified from genomic DNA of each BXD/Ty RI strain.
T6263 680-684 WRB denotes When
T6264 685-693 JJ denotes possible
T6266 693-695 , denotes ,
T6267 695-701 JJ denotes unique
T6269 702-713 NN denotes restriction
T6268 714-719 NNS denotes sites
T6270 720-724 VBD denotes were
T6265 725-735 VBN denotes identified
T6271 736-740 WDT denotes that
T6272 741-755 VBD denotes differentiated
T6273 756-758 NN denotes B6
T6275 759-762 CC denotes and
T6276 763-765 NN denotes D2
T6274 766-773 NNS denotes alleles
T6277 773-775 , denotes ,
T6278 775-778 CC denotes and
T6279 779-782 DT denotes the
T6281 783-796 JJ denotes corresponding
T6282 797-808 NN denotes restriction
T6280 809-822 NNS denotes endonucleases
T6284 823-827 VBD denotes were
T6283 828-832 VBN denotes used
T6285 833-835 IN denotes in
T6286 836-846 JJ denotes diagnostic
T6287 847-854 NNS denotes digests
T6288 855-857 IN denotes of
T6289 858-863 NN denotes Tas2r
T6290 864-869 NNS denotes cDNAs
T6291 870-879 VBN denotes amplified
T6292 880-884 IN denotes from
T6293 885-892 JJ denotes genomic
T6294 893-896 NN denotes DNA
T6295 897-899 IN denotes of
T6296 900-904 DT denotes each
T6298 905-908 NN denotes BXD
T6300 908-909 HYPH denotes /
T6299 909-911 NN denotes Ty
T6301 912-914 JJ denotes RI
T6297 915-921 NN denotes strain
T6302 921-922 . denotes .
T6303 922-1009 sentence denotes For Tas2r120, the absence of a PCR product was considered diagnostic of the D2 allele.
T6304 923-926 IN denotes For
T6306 927-935 NN denotes Tas2r120
T6307 935-937 , denotes ,
T6308 937-940 DT denotes the
T6309 941-948 NN denotes absence
T6310 949-951 IN denotes of
T6311 952-953 DT denotes a
T6313 954-957 NN denotes PCR
T6312 958-965 NN denotes product
T6314 966-969 VBD denotes was
T6305 970-980 VBN denotes considered
T6315 981-991 JJ denotes diagnostic
T6316 992-994 IN denotes of
T6317 995-998 DT denotes the
T6319 999-1001 NN denotes D2
T6318 1002-1008 NN denotes allele
T6320 1008-1009 . denotes .
R4545 T6128 T6129 nsubjpass Oligonucleotides,based
R4546 T6130 T6129 auxpass were,based
R4547 T6131 T6129 prep on,based
R4548 T6132 T6133 amod published,B6
R4549 T6133 T6131 pobj B6,on
R4550 T6134 T6133 compound mTas2r,B6
R4551 T6135 T6133 cc or,B6
R4552 T6136 T6137 nummod 129,SvJ
R4553 T6137 T6139 compound SvJ,sequences
R4554 T6138 T6137 punct /,SvJ
R4555 T6139 T6133 conj sequences,B6
R4556 T6140 T6139 compound cDNA,sequences
R4557 T6141 T6131 cc or,on
R4558 T6142 T6131 conj on,on
R4559 T6143 T6144 det the,genome
R4560 T6144 T6142 pobj genome,on
R4561 T6145 T6144 amod public,genome
R4562 T6146 T6144 compound B6,genome
R4563 T6147 T6129 punct .,based
R4564 T6149 T6150 amod Entire,regions
R4565 T6150 T6152 nsubjpass regions,amplified
R4566 T6151 T6150 compound coding,regions
R4567 T6153 T6150 cc plus,regions
R4568 T6154 T6155 punct ~,50
R4569 T6155 T6156 nummod 50,kb
R4570 T6156 T6150 conj kb,regions
R4571 T6157 T6156 prep of,kb
R4572 T6158 T6159 amod flanking,sequence
R4573 T6159 T6157 pobj sequence,of
R4574 T6160 T6150 prep of,regions
R4575 T6161 T6162 det each,Tas2r
R4576 T6162 T6160 pobj Tas2r,of
R4577 T6163 T6164 amod single,exon
R4578 T6164 T6162 compound exon,Tas2r
R4579 T6165 T6164 punct -,exon
R4580 T6166 T6152 auxpass was,amplified
R4581 T6167 T6152 prep from,amplified
R4582 T6168 T6169 nmod D2,DNA
R4583 T6169 T6167 pobj DNA,from
R4584 T6170 T6168 cc or,D2
R4585 T6171 T6168 conj BXD,D2
R4586 T6172 T6169 amod RI,DNA
R4587 T6173 T6169 amod genomic,DNA
R4588 T6174 T6169 punct (,DNA
R4589 T6175 T6176 compound Jackson,Laboratories
R4590 T6176 T6169 npadvmod Laboratories,DNA
R4591 T6177 T6176 punct ", ",Laboratories
R4592 T6178 T6179 compound Bar,Harbor
R4593 T6179 T6176 npadvmod Harbor,Laboratories
R4594 T6180 T6176 punct ", ",Laboratories
R4595 T6181 T6176 npadvmod ME,Laboratories
R4596 T6182 T6152 punct ),amplified
R4597 T6183 T6152 prep by,amplified
R4598 T6184 T6185 compound polymerase,reaction
R4599 T6185 T6183 pobj reaction,by
R4600 T6186 T6185 compound chain,reaction
R4601 T6187 T6185 punct (,reaction
R4602 T6188 T6185 appos PCR,reaction
R4603 T6189 T6152 punct ),amplified
R4604 T6190 T6152 advcl using,amplified
R4605 T6191 T6192 det a,Complete
R4606 T6192 T6190 dobj Complete,using
R4607 T6193 T6194 amod high,fidelity
R4608 T6194 T6192 compound fidelity,Complete
R4609 T6195 T6194 punct -,fidelity
R4610 T6196 T6192 compound polymerase,Complete
R4611 T6197 T6192 compound TaqPro,Complete
R4612 T6198 T6192 punct (,Complete
R4613 T6199 T6200 compound Denville,Scientific
R4614 T6200 T6192 npadvmod Scientific,Complete
R4615 T6201 T6200 punct ", ",Scientific
R4616 T6202 T6203 compound South,Plainfield
R4617 T6203 T6200 npadvmod Plainfield,Scientific
R4618 T6204 T6200 punct ", ",Scientific
R4619 T6205 T6200 npadvmod NJ,Scientific
R4620 T6206 T6152 punct ),amplified
R4621 T6207 T6152 punct .,amplified
R4622 T6209 T6210 compound PCR,products
R4623 T6210 T6211 nsubjpass products,subcloned
R4624 T6212 T6211 auxpass were,subcloned
R4625 T6213 T6211 prep into,subcloned
R4626 T6214 T6213 pobj pGemT,into
R4627 T6215 T6214 punct -,pGemT
R4628 T6216 T6214 amod Easy,pGemT
R4629 T6217 T6214 punct (,pGemT
R4630 T6218 T6214 npadvmod Promega,pGemT
R4631 T6219 T6218 punct ", ",Promega
R4632 T6220 T6218 npadvmod Madison,Promega
R4633 T6221 T6218 punct ", ",Promega
R4634 T6222 T6218 npadvmod WI,Promega
R4635 T6223 T6211 punct ),subcloned
R4636 T6224 T6211 cc and,subcloned
R4637 T6225 T6211 conj sequenced,subcloned
R4638 T6226 T6225 prep at,sequenced
R4639 T6227 T6228 det the,Core
R4640 T6228 T6226 pobj Core,at
R4641 T6229 T6228 nmod University,Core
R4642 T6230 T6229 prep of,University
R4643 T6231 T6230 pobj Maryland,of
R4644 T6232 T6228 nmod School,Core
R4645 T6233 T6232 prep of,School
R4646 T6234 T6233 pobj Medicine,of
R4647 T6235 T6228 compound Biopolymer,Core
R4648 T6236 T6211 punct .,subcloned
R4649 T6238 T6239 det The,sequences
R4650 T6239 T6240 nsubjpass sequences,compared
R4651 T6241 T6239 prep of,sequences
R4652 T6242 T6243 compound D2,products
R4653 T6243 T6241 pobj products,of
R4654 T6244 T6240 auxpass were,compared
R4655 T6245 T6240 prep to,compared
R4656 T6246 T6247 compound B6,sequences
R4657 T6247 T6245 pobj sequences,to
R4658 T6248 T6247 amod available,sequences
R4659 T6249 T6248 prep in,available
R4660 T6250 T6249 pobj Genbank,in
R4661 T6251 T6252 punct (,see
R4662 T6252 T6240 parataxis see,compared
R4663 T6253 T6254 amod Additional,file
R4664 T6254 T6252 dobj file,see
R4665 T6255 T6254 nummod 2,file
R4666 T6256 T6252 punct ),see
R4667 T6257 T6240 punct ", ",compared
R4668 T6258 T6240 cc and,compared
R4669 T6259 T6260 nsubj polymorphisms,identified
R4670 T6260 T6240 conj identified,compared
R4671 T6261 T6260 punct .,identified
R4672 T6263 T6264 advmod When,possible
R4673 T6264 T6265 advcl possible,identified
R4674 T6266 T6265 punct ", ",identified
R4675 T6267 T6268 amod unique,sites
R4676 T6268 T6265 nsubjpass sites,identified
R4677 T6269 T6268 compound restriction,sites
R4678 T6270 T6265 auxpass were,identified
R4679 T6271 T6272 dep that,differentiated
R4680 T6272 T6265 ccomp differentiated,identified
R4681 T6273 T6274 nmod B6,alleles
R4682 T6274 T6272 dobj alleles,differentiated
R4683 T6275 T6273 cc and,B6
R4684 T6276 T6273 conj D2,B6
R4685 T6277 T6265 punct ", ",identified
R4686 T6278 T6265 cc and,identified
R4687 T6279 T6280 det the,endonucleases
R4688 T6280 T6283 nsubjpass endonucleases,used
R4689 T6281 T6280 amod corresponding,endonucleases
R4690 T6282 T6280 compound restriction,endonucleases
R4691 T6283 T6265 conj used,identified
R4692 T6284 T6283 auxpass were,used
R4693 T6285 T6283 prep in,used
R4694 T6286 T6287 amod diagnostic,digests
R4695 T6287 T6285 pobj digests,in
R4696 T6288 T6287 prep of,digests
R4697 T6289 T6290 compound Tas2r,cDNAs
R4698 T6290 T6288 pobj cDNAs,of
R4699 T6291 T6290 acl amplified,cDNAs
R4700 T6292 T6291 prep from,amplified
R4701 T6293 T6294 amod genomic,DNA
R4702 T6294 T6292 pobj DNA,from
R4703 T6295 T6294 prep of,DNA
R4704 T6296 T6297 det each,strain
R4705 T6297 T6295 pobj strain,of
R4706 T6298 T6299 nmod BXD,Ty
R4707 T6299 T6297 nmod Ty,strain
R4708 T6300 T6299 punct /,Ty
R4709 T6301 T6297 amod RI,strain
R4710 T6302 T6265 punct .,identified
R4711 T6304 T6305 prep For,considered
R4712 T6306 T6304 pobj Tas2r120,For
R4713 T6307 T6305 punct ", ",considered
R4714 T6308 T6309 det the,absence
R4715 T6309 T6305 nsubjpass absence,considered
R4716 T6310 T6309 prep of,absence
R4717 T6311 T6312 det a,product
R4718 T6312 T6310 pobj product,of
R4719 T6313 T6312 compound PCR,product
R4720 T6314 T6305 auxpass was,considered
R4721 T6315 T6305 oprd diagnostic,considered
R4722 T6316 T6315 prep of,diagnostic
R4723 T6317 T6318 det the,allele
R4724 T6318 T6316 pobj allele,of
R4725 T6319 T6318 compound D2,allele
R4726 T6320 T6305 punct .,considered