Id |
Subject |
Object |
Predicate |
Lexical cue |
T6258 |
0-5 |
JJ |
denotes |
Total |
T6259 |
6-9 |
NN |
denotes |
RNA |
T6261 |
10-13 |
VBD |
denotes |
was |
T6260 |
14-23 |
VBN |
denotes |
extracted |
T6262 |
24-28 |
IN |
denotes |
from |
T6263 |
29-32 |
DT |
denotes |
the |
T6265 |
33-39 |
JJ |
denotes |
entire |
T6266 |
40-45 |
JJ |
denotes |
small |
T6264 |
46-55 |
NN |
denotes |
intestine |
T6267 |
56-58 |
IN |
denotes |
as |
T6269 |
59-69 |
RB |
denotes |
previously |
T6268 |
70-79 |
VBN |
denotes |
described |
T6270 |
80-81 |
-LRB- |
denotes |
[ |
T6271 |
81-83 |
CD |
denotes |
21 |
T6272 |
83-84 |
-RRB- |
denotes |
] |
T6273 |
84-85 |
. |
denotes |
. |
T6274 |
85-210 |
sentence |
denotes |
Quantitative, real-time RT-PCR was used to measure expression of specific genes using the previously described primers [21]. |
T6275 |
86-98 |
JJ |
denotes |
Quantitative |
T6277 |
98-100 |
, |
denotes |
, |
T6278 |
100-104 |
JJ |
denotes |
real |
T6280 |
104-105 |
HYPH |
denotes |
- |
T6279 |
105-109 |
NN |
denotes |
time |
T6281 |
110-112 |
NN |
denotes |
RT |
T6282 |
112-113 |
HYPH |
denotes |
- |
T6276 |
113-116 |
NN |
denotes |
PCR |
T6284 |
117-120 |
VBD |
denotes |
was |
T6283 |
121-125 |
VBN |
denotes |
used |
T6285 |
126-128 |
TO |
denotes |
to |
T6286 |
129-136 |
VB |
denotes |
measure |
T6287 |
137-147 |
NN |
denotes |
expression |
T6288 |
148-150 |
IN |
denotes |
of |
T6289 |
151-159 |
JJ |
denotes |
specific |
T6290 |
160-165 |
NNS |
denotes |
genes |
T6291 |
166-171 |
VBG |
denotes |
using |
T6292 |
172-175 |
DT |
denotes |
the |
T6294 |
176-186 |
RB |
denotes |
previously |
T6295 |
187-196 |
VBN |
denotes |
described |
T6293 |
197-204 |
NNS |
denotes |
primers |
T6296 |
205-206 |
-LRB- |
denotes |
[ |
T6297 |
206-208 |
CD |
denotes |
21 |
T6298 |
208-209 |
-RRB- |
denotes |
] |
T6299 |
209-210 |
. |
denotes |
. |
T6300 |
210-341 |
sentence |
denotes |
Values were normalized to GAPDH mRNA and expression of this housekeeping gene is not altered in the CF mouse small intestine [21]. |
T6301 |
211-217 |
NNS |
denotes |
Values |
T6303 |
218-222 |
VBD |
denotes |
were |
T6302 |
223-233 |
VBN |
denotes |
normalized |
T6304 |
234-236 |
IN |
denotes |
to |
T6305 |
237-242 |
NN |
denotes |
GAPDH |
T6306 |
243-247 |
NN |
denotes |
mRNA |
T6307 |
248-251 |
CC |
denotes |
and |
T6308 |
252-262 |
NN |
denotes |
expression |
T6310 |
263-265 |
IN |
denotes |
of |
T6311 |
266-270 |
DT |
denotes |
this |
T6313 |
271-283 |
NN |
denotes |
housekeeping |
T6312 |
284-288 |
NN |
denotes |
gene |
T6314 |
289-291 |
VBZ |
denotes |
is |
T6315 |
292-295 |
RB |
denotes |
not |
T6309 |
296-303 |
VBN |
denotes |
altered |
T6316 |
304-306 |
IN |
denotes |
in |
T6317 |
307-310 |
DT |
denotes |
the |
T6319 |
311-313 |
NN |
denotes |
CF |
T6320 |
314-319 |
NN |
denotes |
mouse |
T6321 |
320-325 |
JJ |
denotes |
small |
T6318 |
326-335 |
NN |
denotes |
intestine |
T6322 |
336-337 |
-LRB- |
denotes |
[ |
T6323 |
337-339 |
CD |
denotes |
21 |
T6324 |
339-340 |
-RRB- |
denotes |
] |
T6325 |
340-341 |
. |
denotes |
. |
T6326 |
341-516 |
sentence |
denotes |
Expression of the major intestinal mucin, Muc2, was also measured using the forward (5'-GAC TTC GAT GGA CAC TGC TC-3') and reverse (5'-CAC GGT GTT TAT CTA CCA AC-3') primers. |
T6327 |
342-352 |
NN |
denotes |
Expression |
T6329 |
353-355 |
IN |
denotes |
of |
T6330 |
356-359 |
DT |
denotes |
the |
T6332 |
360-365 |
JJ |
denotes |
major |
T6333 |
366-376 |
JJ |
denotes |
intestinal |
T6331 |
377-382 |
NN |
denotes |
mucin |
T6334 |
382-384 |
, |
denotes |
, |
T6335 |
384-388 |
NN |
denotes |
Muc2 |
T6336 |
388-390 |
, |
denotes |
, |
T6337 |
390-393 |
VBD |
denotes |
was |
T6338 |
394-398 |
RB |
denotes |
also |
T6328 |
399-407 |
VBN |
denotes |
measured |
T6339 |
408-413 |
VBG |
denotes |
using |
T6340 |
414-417 |
DT |
denotes |
the |
T6342 |
418-425 |
JJ |
denotes |
forward |
T6343 |
426-427 |
-LRB- |
denotes |
( |
T6345 |
427-428 |
CD |
denotes |
5 |
T6346 |
428-429 |
SYM |
denotes |
' |
T6347 |
429-430 |
HYPH |
denotes |
- |
T6348 |
430-433 |
NN |
denotes |
GAC |
T6349 |
434-437 |
NN |
denotes |
TTC |
T6350 |
438-441 |
NN |
denotes |
GAT |
T6351 |
442-445 |
NN |
denotes |
GGA |
T6352 |
446-449 |
NN |
denotes |
CAC |
T6353 |
450-453 |
NN |
denotes |
TGC |
T6344 |
454-456 |
NN |
denotes |
TC |
T6354 |
456-457 |
HYPH |
denotes |
- |
T6355 |
457-458 |
CD |
denotes |
3 |
T6356 |
458-459 |
SYM |
denotes |
' |
T6357 |
459-460 |
-RRB- |
denotes |
) |
T6358 |
461-464 |
CC |
denotes |
and |
T6359 |
465-472 |
JJ |
denotes |
reverse |
T6360 |
473-474 |
-LRB- |
denotes |
( |
T6362 |
474-475 |
CD |
denotes |
5 |
T6363 |
475-476 |
SYM |
denotes |
' |
T6364 |
476-477 |
HYPH |
denotes |
- |
T6365 |
477-480 |
NN |
denotes |
CAC |
T6366 |
481-484 |
NN |
denotes |
GGT |
T6367 |
485-488 |
NN |
denotes |
GTT |
T6368 |
489-492 |
NN |
denotes |
TAT |
T6369 |
493-496 |
NN |
denotes |
CTA |
T6370 |
497-500 |
NN |
denotes |
CCA |
T6361 |
501-503 |
NN |
denotes |
AC |
T6371 |
503-504 |
HYPH |
denotes |
- |
T6372 |
504-505 |
CD |
denotes |
3 |
T6373 |
505-506 |
SYM |
denotes |
' |
T6374 |
506-507 |
-RRB- |
denotes |
) |
T6341 |
508-515 |
NNS |
denotes |
primers |
T6375 |
515-516 |
. |
denotes |
. |
R4159 |
T6258 |
T6259 |
amod |
Total,RNA |
R4160 |
T6259 |
T6260 |
nsubjpass |
RNA,extracted |
R4161 |
T6261 |
T6260 |
auxpass |
was,extracted |
R4162 |
T6262 |
T6260 |
prep |
from,extracted |
R4163 |
T6263 |
T6264 |
det |
the,intestine |
R4164 |
T6264 |
T6262 |
pobj |
intestine,from |
R4165 |
T6265 |
T6264 |
amod |
entire,intestine |
R4166 |
T6266 |
T6264 |
amod |
small,intestine |
R4167 |
T6267 |
T6268 |
mark |
as,described |
R4168 |
T6268 |
T6260 |
advcl |
described,extracted |
R4169 |
T6269 |
T6268 |
advmod |
previously,described |
R4170 |
T6270 |
T6271 |
punct |
[,21 |
R4171 |
T6271 |
T6260 |
parataxis |
21,extracted |
R4172 |
T6272 |
T6271 |
punct |
],21 |
R4173 |
T6273 |
T6260 |
punct |
.,extracted |
R4174 |
T6275 |
T6276 |
amod |
Quantitative,PCR |
R4175 |
T6276 |
T6283 |
nsubjpass |
PCR,used |
R4176 |
T6277 |
T6276 |
punct |
", ",PCR |
R4177 |
T6278 |
T6279 |
amod |
real,time |
R4178 |
T6279 |
T6276 |
compound |
time,PCR |
R4179 |
T6280 |
T6279 |
punct |
-,time |
R4180 |
T6281 |
T6276 |
compound |
RT,PCR |
R4181 |
T6282 |
T6276 |
punct |
-,PCR |
R4182 |
T6284 |
T6283 |
auxpass |
was,used |
R4183 |
T6285 |
T6286 |
aux |
to,measure |
R4184 |
T6286 |
T6283 |
advcl |
measure,used |
R4185 |
T6287 |
T6286 |
dobj |
expression,measure |
R4186 |
T6288 |
T6287 |
prep |
of,expression |
R4187 |
T6289 |
T6290 |
amod |
specific,genes |
R4188 |
T6290 |
T6288 |
pobj |
genes,of |
R4189 |
T6291 |
T6286 |
advcl |
using,measure |
R4190 |
T6292 |
T6293 |
det |
the,primers |
R4191 |
T6293 |
T6291 |
dobj |
primers,using |
R4192 |
T6294 |
T6295 |
advmod |
previously,described |
R4193 |
T6295 |
T6293 |
amod |
described,primers |
R4194 |
T6296 |
T6297 |
punct |
[,21 |
R4195 |
T6297 |
T6283 |
parataxis |
21,used |
R4196 |
T6298 |
T6297 |
punct |
],21 |
R4197 |
T6299 |
T6283 |
punct |
.,used |
R4198 |
T6301 |
T6302 |
nsubjpass |
Values,normalized |
R4199 |
T6303 |
T6302 |
auxpass |
were,normalized |
R4200 |
T6304 |
T6302 |
prep |
to,normalized |
R4201 |
T6305 |
T6306 |
compound |
GAPDH,mRNA |
R4202 |
T6306 |
T6304 |
pobj |
mRNA,to |
R4203 |
T6307 |
T6302 |
cc |
and,normalized |
R4204 |
T6308 |
T6309 |
nsubjpass |
expression,altered |
R4205 |
T6309 |
T6302 |
conj |
altered,normalized |
R4206 |
T6310 |
T6308 |
prep |
of,expression |
R4207 |
T6311 |
T6312 |
det |
this,gene |
R4208 |
T6312 |
T6310 |
pobj |
gene,of |
R4209 |
T6313 |
T6312 |
compound |
housekeeping,gene |
R4210 |
T6314 |
T6309 |
auxpass |
is,altered |
R4211 |
T6315 |
T6309 |
neg |
not,altered |
R4212 |
T6316 |
T6309 |
prep |
in,altered |
R4213 |
T6317 |
T6318 |
det |
the,intestine |
R4214 |
T6318 |
T6316 |
pobj |
intestine,in |
R4215 |
T6319 |
T6320 |
nmod |
CF,mouse |
R4216 |
T6320 |
T6318 |
nmod |
mouse,intestine |
R4217 |
T6321 |
T6318 |
amod |
small,intestine |
R4218 |
T6322 |
T6323 |
punct |
[,21 |
R4219 |
T6323 |
T6309 |
parataxis |
21,altered |
R4220 |
T6324 |
T6323 |
punct |
],21 |
R4221 |
T6325 |
T6309 |
punct |
.,altered |
R4222 |
T6327 |
T6328 |
nsubjpass |
Expression,measured |
R4223 |
T6329 |
T6327 |
prep |
of,Expression |
R4224 |
T6330 |
T6331 |
det |
the,mucin |
R4225 |
T6331 |
T6329 |
pobj |
mucin,of |
R4226 |
T6332 |
T6331 |
amod |
major,mucin |
R4227 |
T6333 |
T6331 |
amod |
intestinal,mucin |
R4228 |
T6334 |
T6331 |
punct |
", ",mucin |
R4229 |
T6335 |
T6331 |
appos |
Muc2,mucin |
R4230 |
T6336 |
T6328 |
punct |
", ",measured |
R4231 |
T6337 |
T6328 |
auxpass |
was,measured |
R4232 |
T6338 |
T6328 |
advmod |
also,measured |
R4233 |
T6339 |
T6328 |
advcl |
using,measured |
R4234 |
T6340 |
T6341 |
det |
the,primers |
R4235 |
T6341 |
T6339 |
dobj |
primers,using |
R4236 |
T6342 |
T6341 |
amod |
forward,primers |
R4237 |
T6343 |
T6344 |
punct |
(,TC |
R4238 |
T6344 |
T6342 |
parataxis |
TC,forward |
R4239 |
T6345 |
T6344 |
nummod |
5,TC |
R4240 |
T6346 |
T6345 |
punct |
',5 |
R4241 |
T6347 |
T6344 |
punct |
-,TC |
R4242 |
T6348 |
T6344 |
compound |
GAC,TC |
R4243 |
T6349 |
T6344 |
compound |
TTC,TC |
R4244 |
T6350 |
T6344 |
compound |
GAT,TC |
R4245 |
T6351 |
T6344 |
compound |
GGA,TC |
R4246 |
T6352 |
T6344 |
compound |
CAC,TC |
R4247 |
T6353 |
T6344 |
compound |
TGC,TC |
R4248 |
T6354 |
T6344 |
punct |
-,TC |
R4249 |
T6355 |
T6344 |
nummod |
3,TC |
R4250 |
T6356 |
T6344 |
punct |
',TC |
R4251 |
T6357 |
T6344 |
punct |
),TC |
R4252 |
T6358 |
T6342 |
cc |
and,forward |
R4253 |
T6359 |
T6342 |
conj |
reverse,forward |
R4254 |
T6360 |
T6361 |
punct |
(,AC |
R4255 |
T6361 |
T6359 |
parataxis |
AC,reverse |
R4256 |
T6362 |
T6361 |
nummod |
5,AC |
R4257 |
T6363 |
T6362 |
punct |
',5 |
R4258 |
T6364 |
T6361 |
punct |
-,AC |
R4259 |
T6365 |
T6361 |
compound |
CAC,AC |
R4260 |
T6366 |
T6361 |
compound |
GGT,AC |
R4261 |
T6367 |
T6361 |
compound |
GTT,AC |
R4262 |
T6368 |
T6361 |
compound |
TAT,AC |
R4263 |
T6369 |
T6361 |
compound |
CTA,AC |
R4264 |
T6370 |
T6361 |
compound |
CCA,AC |
R4265 |
T6371 |
T6361 |
punct |
-,AC |
R4266 |
T6372 |
T6361 |
nummod |
3,AC |
R4267 |
T6373 |
T6361 |
punct |
',AC |
R4268 |
T6374 |
T6361 |
punct |
),AC |
R4269 |
T6375 |
T6328 |
punct |
.,measured |