craft-sa-dev Home  

PMC:116589 / 4819-5569 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1521 0-1 DT denotes A
T1522 2-14 NN denotes breakthrough
T1524 15-17 IN denotes in
T1525 18-21 DT denotes the
T1526 22-34 NN denotes availability
T1527 35-37 IN denotes of
T1528 38-48 RB denotes spectrally
T1529 49-57 JJ denotes distinct
T1531 58-73 JJ denotes autofluorescent
T1530 74-82 NN denotes proteins
T1523 83-87 VBD denotes came
T1532 88-92 IN denotes with
T1533 93-96 DT denotes the
T1534 97-104 NN denotes cloning
T1535 105-107 IN denotes of
T1536 108-111 CD denotes six
T1538 112-121 NN denotes anthozoan
T1539 122-133 JJ denotes fluorescent
T1537 134-142 NN denotes proteins
T1540 143-146 RB denotes all
T1541 147-153 VBG denotes having
T1542 154-156 CD denotes 20
T1544 156-157 SYM denotes –
T1543 157-159 CD denotes 30
T1545 159-160 NN denotes %
T1546 161-169 NN denotes identity
T1547 170-172 IN denotes to
T1548 173-178 NN denotes wtGFP
T1549 179-180 -LRB- denotes [
T1550 180-182 CD denotes 12
T1551 182-183 -RRB- denotes ]
T1552 183-184 . denotes .
T1553 184-449 sentence denotes Of these, DsRed1 (referred to as drFP585 by Matz et al., [12]), a 28 kDa protein isolated from the IndoPacific sea anemone Discosoma sp, posseses the longest excitation (558 nm) and emission (583 nm) wavelength maxima known for a wild-type autofluorescent protein.
T1554 185-187 IN denotes Of
T1556 188-193 DT denotes these
T1557 193-195 , denotes ,
T1558 195-201 NN denotes DsRed1
T1559 202-203 -LRB- denotes (
T1560 203-211 VBN denotes referred
T1561 212-214 IN denotes to
T1562 215-217 IN denotes as
T1563 218-225 NN denotes drFP585
T1564 226-228 IN denotes by
T1565 229-233 NNP denotes Matz
T1566 234-236 FW denotes et
T1567 237-240 FW denotes al.
T1568 240-242 , denotes ,
T1569 242-243 -LRB- denotes [
T1570 243-245 CD denotes 12
T1571 245-246 -RRB- denotes ]
T1572 246-247 -RRB- denotes )
T1573 247-249 , denotes ,
T1574 249-250 DT denotes a
T1576 251-253 CD denotes 28
T1577 254-257 NN denotes kDa
T1575 258-265 NN denotes protein
T1578 266-274 VBN denotes isolated
T1579 275-279 IN denotes from
T1580 280-283 DT denotes the
T1582 284-295 NNP denotes IndoPacific
T1583 296-299 NN denotes sea
T1581 300-307 NN denotes anemone
T1584 308-317 NNP denotes Discosoma
T1585 318-320 NNP denotes sp
T1586 320-322 , denotes ,
T1555 322-330 VBZ denotes posseses
T1587 331-334 DT denotes the
T1589 335-342 JJS denotes longest
T1590 343-353 NN denotes excitation
T1591 354-355 -LRB- denotes (
T1593 355-358 CD denotes 558
T1592 359-361 NN denotes nm
T1594 361-362 -RRB- denotes )
T1595 363-366 CC denotes and
T1596 367-375 NN denotes emission
T1597 376-377 -LRB- denotes (
T1599 377-380 CD denotes 583
T1598 381-383 NN denotes nm
T1600 383-384 -RRB- denotes )
T1601 385-395 NN denotes wavelength
T1588 396-402 NNS denotes maxima
T1602 403-408 VBN denotes known
T1603 409-412 IN denotes for
T1604 413-414 DT denotes a
T1606 415-419 JJ denotes wild
T1608 419-420 HYPH denotes -
T1607 420-424 NN denotes type
T1609 425-440 JJ denotes autofluorescent
T1605 441-448 NN denotes protein
T1610 448-449 . denotes .
T1611 449-662 sentence denotes The sequence of DsRed1 has recently been modified and optimized for high levels of expression in mammalian cells by the introduction of 144 silent base pair changes corresponding to human codon usage preferences.
T1612 450-453 DT denotes The
T1613 454-462 NN denotes sequence
T1615 463-465 IN denotes of
T1616 466-472 NN denotes DsRed1
T1617 473-476 VBZ denotes has
T1618 477-485 RB denotes recently
T1619 486-490 VBN denotes been
T1614 491-499 VBN denotes modified
T1620 500-503 CC denotes and
T1621 504-513 VBN denotes optimized
T1622 514-517 IN denotes for
T1623 518-522 JJ denotes high
T1624 523-529 NNS denotes levels
T1625 530-532 IN denotes of
T1626 533-543 NN denotes expression
T1627 544-546 IN denotes in
T1628 547-556 JJ denotes mammalian
T1629 557-562 NNS denotes cells
T1630 563-565 IN denotes by
T1631 566-569 DT denotes the
T1632 570-582 NN denotes introduction
T1633 583-585 IN denotes of
T1634 586-589 CD denotes 144
T1636 590-596 JJ denotes silent
T1638 597-601 NN denotes base
T1637 602-606 NN denotes pair
T1635 607-614 NNS denotes changes
T1639 615-628 VBG denotes corresponding
T1640 629-631 IN denotes to
T1641 632-637 JJ denotes human
T1642 638-643 NN denotes codon
T1643 644-649 NN denotes usage
T1644 650-661 NNS denotes preferences
T1645 661-662 . denotes .
T1646 662-750 sentence denotes We therefore also tested this novel red fluorescent protein (RFP) in ES cells and mice.
T1647 663-665 PRP denotes We
T1649 666-675 RB denotes therefore
T1650 676-680 RB denotes also
T1648 681-687 VBN denotes tested
T1651 688-692 DT denotes this
T1653 693-698 JJ denotes novel
T1654 699-702 JJ denotes red
T1655 703-714 JJ denotes fluorescent
T1652 715-722 NN denotes protein
T1656 723-724 -LRB- denotes (
T1657 724-727 NN denotes RFP
T1658 727-728 -RRB- denotes )
T1659 729-731 IN denotes in
T1660 732-734 NN denotes ES
T1661 735-740 NNS denotes cells
T1662 741-744 CC denotes and
T1663 745-749 NNS denotes mice
T1664 749-750 . denotes .
R819 T1521 T1522 det A,breakthrough
R820 T1522 T1523 nsubj breakthrough,came
R821 T1524 T1522 prep in,breakthrough
R822 T1525 T1526 det the,availability
R823 T1526 T1524 pobj availability,in
R824 T1527 T1526 prep of,availability
R825 T1528 T1529 advmod spectrally,distinct
R826 T1529 T1530 amod distinct,proteins
R827 T1530 T1527 pobj proteins,of
R828 T1531 T1530 amod autofluorescent,proteins
R829 T1532 T1523 prep with,came
R830 T1533 T1534 det the,cloning
R831 T1534 T1532 pobj cloning,with
R832 T1535 T1534 prep of,cloning
R833 T1536 T1537 nummod six,proteins
R834 T1537 T1535 pobj proteins,of
R835 T1538 T1537 nmod anthozoan,proteins
R836 T1539 T1537 amod fluorescent,proteins
R837 T1540 T1541 advmod all,having
R838 T1541 T1537 relcl having,proteins
R839 T1542 T1543 quantmod 20,30
R840 T1543 T1545 nummod 30,%
R841 T1544 T1543 punct –,30
R842 T1545 T1546 compound %,identity
R843 T1546 T1541 dobj identity,having
R844 T1547 T1546 prep to,identity
R845 T1548 T1547 pobj wtGFP,to
R846 T1549 T1550 punct [,12
R847 T1550 T1523 parataxis 12,came
R848 T1551 T1550 punct ],12
R849 T1552 T1523 punct .,came
R850 T1554 T1555 prep Of,posseses
R851 T1556 T1554 pobj these,Of
R852 T1557 T1555 punct ", ",posseses
R853 T1558 T1555 nsubj DsRed1,posseses
R854 T1559 T1558 punct (,DsRed1
R855 T1560 T1558 acl referred,DsRed1
R856 T1561 T1560 prep to,referred
R857 T1562 T1560 prep as,referred
R858 T1563 T1562 pobj drFP585,as
R859 T1564 T1560 agent by,referred
R860 T1565 T1564 pobj Matz,by
R861 T1566 T1567 advmod et,al.
R862 T1567 T1565 advmod al.,Matz
R863 T1568 T1560 punct ", ",referred
R864 T1569 T1570 punct [,12
R865 T1570 T1560 parataxis 12,referred
R866 T1571 T1570 punct ],12
R867 T1572 T1558 punct ),DsRed1
R868 T1573 T1558 punct ", ",DsRed1
R869 T1574 T1575 det a,protein
R870 T1575 T1558 appos protein,DsRed1
R871 T1576 T1577 nummod 28,kDa
R872 T1577 T1575 compound kDa,protein
R873 T1578 T1575 acl isolated,protein
R874 T1579 T1578 prep from,isolated
R875 T1580 T1581 det the,anemone
R876 T1581 T1579 pobj anemone,from
R877 T1582 T1581 compound IndoPacific,anemone
R878 T1583 T1581 compound sea,anemone
R879 T1584 T1585 compound Discosoma,sp
R880 T1585 T1581 appos sp,anemone
R881 T1586 T1555 punct ", ",posseses
R882 T1587 T1588 det the,maxima
R883 T1588 T1555 dobj maxima,posseses
R884 T1589 T1588 amod longest,maxima
R885 T1590 T1588 nmod excitation,maxima
R886 T1591 T1592 punct (,nm
R887 T1592 T1590 parataxis nm,excitation
R888 T1593 T1592 nummod 558,nm
R889 T1594 T1592 punct ),nm
R890 T1595 T1590 cc and,excitation
R891 T1596 T1590 conj emission,excitation
R892 T1597 T1598 punct (,nm
R893 T1598 T1596 parataxis nm,emission
R894 T1599 T1598 nummod 583,nm
R895 T1600 T1598 punct ),nm
R896 T1601 T1588 compound wavelength,maxima
R897 T1602 T1588 acl known,maxima
R898 T1603 T1602 prep for,known
R899 T1604 T1605 det a,protein
R900 T1605 T1603 pobj protein,for
R901 T1606 T1607 amod wild,type
R902 T1607 T1605 nmod type,protein
R903 T1608 T1607 punct -,type
R904 T1609 T1605 amod autofluorescent,protein
R905 T1610 T1555 punct .,posseses
R906 T1612 T1613 det The,sequence
R907 T1613 T1614 nsubjpass sequence,modified
R908 T1615 T1613 prep of,sequence
R909 T1616 T1615 pobj DsRed1,of
R910 T1617 T1614 aux has,modified
R911 T1618 T1614 advmod recently,modified
R912 T1619 T1614 auxpass been,modified
R913 T1620 T1614 cc and,modified
R914 T1621 T1614 conj optimized,modified
R915 T1622 T1621 prep for,optimized
R916 T1623 T1624 amod high,levels
R917 T1624 T1622 pobj levels,for
R918 T1625 T1624 prep of,levels
R919 T1626 T1625 pobj expression,of
R920 T1627 T1624 prep in,levels
R921 T1628 T1629 amod mammalian,cells
R922 T1629 T1627 pobj cells,in
R923 T1630 T1621 prep by,optimized
R924 T1631 T1632 det the,introduction
R925 T1632 T1630 pobj introduction,by
R926 T1633 T1632 prep of,introduction
R927 T1634 T1635 nummod 144,changes
R928 T1635 T1633 pobj changes,of
R929 T1636 T1637 amod silent,pair
R930 T1637 T1635 compound pair,changes
R931 T1638 T1637 compound base,pair
R932 T1639 T1635 acl corresponding,changes
R933 T1640 T1639 prep to,corresponding
R934 T1641 T1642 amod human,codon
R935 T1642 T1643 compound codon,usage
R936 T1643 T1644 compound usage,preferences
R937 T1644 T1640 pobj preferences,to
R938 T1645 T1614 punct .,modified
R939 T1647 T1648 nsubj We,tested
R940 T1649 T1648 advmod therefore,tested
R941 T1650 T1648 advmod also,tested
R942 T1651 T1652 det this,protein
R943 T1652 T1648 dobj protein,tested
R944 T1653 T1652 amod novel,protein
R945 T1654 T1652 amod red,protein
R946 T1655 T1652 amod fluorescent,protein
R947 T1656 T1652 punct (,protein
R948 T1657 T1652 appos RFP,protein
R949 T1658 T1648 punct ),tested
R950 T1659 T1648 prep in,tested
R951 T1660 T1661 compound ES,cells
R952 T1661 T1659 pobj cells,in
R953 T1662 T1661 cc and,cells
R954 T1663 T1661 conj mice,cells
R955 T1664 T1648 punct .,tested