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PMC:116589 / 2146-4182 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T646 11-14 DT denotes The
T647 15-19 NN denotes ease
T649 20-22 IN denotes of
T650 23-35 VBG denotes manipulating
T651 36-39 PRP$ denotes its
T652 40-46 NN denotes genome
T653 47-50 VBZ denotes has
T654 51-57 VBN denotes helped
T648 58-67 VB denotes establish
T655 68-71 DT denotes the
T656 72-77 NN denotes mouse
T657 78-80 IN denotes as
T658 81-84 DT denotes the
T660 85-92 JJ denotes premier
T661 93-98 NN denotes model
T659 99-107 NN denotes organism
T662 108-111 IN denotes for
T663 112-121 JJ denotes mammalian
T665 122-129 JJ denotes genetic
T664 130-137 NNS denotes studies
T666 137-138 . denotes .
T667 138-287 sentence denotes Both directed and random mutagenesis approaches, including the technologies of transgenesis and gene targeting in ES cells, have become commonplace.
T668 139-143 CC denotes Both
T669 144-152 JJ denotes directed
T671 153-156 CC denotes and
T672 157-163 JJ denotes random
T673 164-175 NN denotes mutagenesis
T670 176-186 NNS denotes approaches
T675 186-188 , denotes ,
T676 188-197 VBG denotes including
T677 198-201 DT denotes the
T678 202-214 NNS denotes technologies
T679 215-217 IN denotes of
T680 218-230 NN denotes transgenesis
T681 231-234 CC denotes and
T682 235-239 NN denotes gene
T683 240-249 NN denotes targeting
T684 250-252 IN denotes in
T685 253-255 NN denotes ES
T686 256-261 NNS denotes cells
T687 261-263 , denotes ,
T688 263-267 VBP denotes have
T674 268-274 VBN denotes become
T689 275-286 JJ denotes commonplace
T690 286-287 . denotes .
T691 287-454 sentence denotes Genetically manipulated mice, often incorporating gene-based reporters, are frequently being used to model and understand mammalian development and disease processes.
T692 288-299 RB denotes Genetically
T693 300-311 VBN denotes manipulated
T694 312-316 NNS denotes mice
T696 316-318 , denotes ,
T697 318-323 RB denotes often
T698 324-337 VBG denotes incorporating
T699 338-342 NN denotes gene
T701 342-343 HYPH denotes -
T700 343-348 VBN denotes based
T702 349-358 NNS denotes reporters
T703 358-360 , denotes ,
T704 360-363 VBP denotes are
T705 364-374 RB denotes frequently
T706 375-380 VBG denotes being
T695 381-385 VBN denotes used
T707 386-388 TO denotes to
T708 389-394 VB denotes model
T709 395-398 CC denotes and
T710 399-409 VB denotes understand
T711 410-419 JJ denotes mammalian
T712 420-431 NN denotes development
T713 432-435 CC denotes and
T714 436-443 NN denotes disease
T715 444-453 NNS denotes processes
T716 453-454 . denotes .
T717 454-722 sentence denotes Fluorescent protein reporters currently represent a superior alternative to other gene-based reporters such as the bacterial lacZ or human placental alkaline phosphatase in that their visualization is non-invasive, and as such does not require chromogenic substrates.
T718 455-466 JJ denotes Fluorescent
T719 467-474 NN denotes protein
T720 475-484 NNS denotes reporters
T722 485-494 RB denotes currently
T721 495-504 VBP denotes represent
T723 505-506 DT denotes a
T725 507-515 JJ denotes superior
T724 516-527 NN denotes alternative
T726 528-530 IN denotes to
T727 531-536 JJ denotes other
T729 537-541 NN denotes gene
T731 541-542 HYPH denotes -
T730 542-547 VBN denotes based
T728 548-557 NNS denotes reporters
T732 558-562 JJ denotes such
T733 563-565 IN denotes as
T734 566-569 DT denotes the
T736 570-579 JJ denotes bacterial
T735 580-584 NN denotes lacZ
T737 585-587 CC denotes or
T738 588-593 JJ denotes human
T739 594-603 JJ denotes placental
T741 604-612 JJ denotes alkaline
T740 613-624 NN denotes phosphatase
T742 625-627 IN denotes in
T744 628-632 DT denotes that
T745 633-638 PRP$ denotes their
T746 639-652 NN denotes visualization
T743 653-655 VBZ denotes is
T747 656-668 JJ denotes non-invasive
T748 668-670 , denotes ,
T749 670-673 CC denotes and
T750 674-676 IN denotes as
T752 677-681 JJ denotes such
T753 682-686 VBZ denotes does
T754 687-690 RB denotes not
T751 691-698 VB denotes require
T755 699-710 JJ denotes chromogenic
T756 711-721 NNS denotes substrates
T757 721-722 . denotes .
T758 722-844 sentence denotes Fluorescence can be monitored in real-time in vivo and in situ, and has the added advantage in that it can be quantified.
T759 723-735 NN denotes Fluorescence
T761 736-739 MD denotes can
T762 740-742 VB denotes be
T760 743-752 VBN denotes monitored
T763 753-755 IN denotes in
T764 756-760 JJ denotes real
T766 760-761 HYPH denotes -
T765 761-765 NN denotes time
T767 766-768 FW denotes in
T768 769-773 FW denotes vivo
T769 774-777 CC denotes and
T770 778-780 FW denotes in
T771 781-785 FW denotes situ
T772 785-787 , denotes ,
T773 787-790 CC denotes and
T774 791-794 VBZ denotes has
T775 795-798 DT denotes the
T777 799-804 VBN denotes added
T776 805-814 NN denotes advantage
T778 815-817 IN denotes in
T780 818-822 IN denotes that
T781 823-825 PRP denotes it
T782 826-829 MD denotes can
T783 830-832 VB denotes be
T779 833-843 VBN denotes quantified
T784 843-844 . denotes .
T785 844-1014 sentence denotes The prototype fluorescent protein reporter is green fluorescent protein (GFP, reviewed in [3]), which is derived from the bioluminescent jellyfish Aequorea Victoria [1].
T786 845-848 DT denotes The
T788 849-858 NN denotes prototype
T789 859-870 JJ denotes fluorescent
T790 871-878 NN denotes protein
T787 879-887 NN denotes reporter
T791 888-890 VBZ denotes is
T792 891-896 JJ denotes green
T794 897-908 JJ denotes fluorescent
T793 909-916 NN denotes protein
T795 917-918 -LRB- denotes (
T796 918-921 NN denotes GFP
T797 921-923 , denotes ,
T798 923-931 VBN denotes reviewed
T799 932-934 IN denotes in
T800 935-936 -LRB- denotes [
T801 936-937 CD denotes 3
T802 937-938 -RRB- denotes ]
T803 938-939 -RRB- denotes )
T804 939-941 , denotes ,
T805 941-946 WDT denotes which
T807 947-949 VBZ denotes is
T806 950-957 VBN denotes derived
T808 958-962 IN denotes from
T809 963-966 DT denotes the
T811 967-981 JJ denotes bioluminescent
T810 982-991 NN denotes jellyfish
T812 992-1000 NNP denotes Aequorea
T813 1001-1009 NNP denotes Victoria
T814 1010-1011 -LRB- denotes [
T815 1011-1012 CD denotes 1
T816 1012-1013 -RRB- denotes ]
T817 1013-1014 . denotes .
T818 1014-1236 sentence denotes Green fluorescent variants of wild type GFP (wtGFP) with improved thermostability and fluorescence emission, including enhanced green fluorescent protein (EGFP, [4]), and mMGFP [5], have gained popularity for use in mice.
T819 1015-1020 JJ denotes Green
T821 1021-1032 JJ denotes fluorescent
T820 1033-1041 NNS denotes variants
T823 1042-1044 IN denotes of
T824 1045-1049 JJ denotes wild
T825 1050-1054 NN denotes type
T826 1055-1058 NN denotes GFP
T827 1059-1060 -LRB- denotes (
T828 1060-1065 NN denotes wtGFP
T829 1065-1066 -RRB- denotes )
T830 1067-1071 IN denotes with
T831 1072-1080 VBN denotes improved
T832 1081-1096 NN denotes thermostability
T833 1097-1100 CC denotes and
T834 1101-1113 NN denotes fluorescence
T835 1114-1122 NN denotes emission
T836 1122-1124 , denotes ,
T837 1124-1133 VBG denotes including
T838 1134-1142 VBN denotes enhanced
T840 1143-1148 JJ denotes green
T841 1149-1160 JJ denotes fluorescent
T839 1161-1168 NN denotes protein
T842 1169-1170 -LRB- denotes (
T843 1170-1174 NN denotes EGFP
T844 1174-1176 , denotes ,
T845 1176-1177 -LRB- denotes [
T846 1177-1178 CD denotes 4
T847 1178-1179 -RRB- denotes ]
T848 1179-1180 -RRB- denotes )
T849 1180-1182 , denotes ,
T850 1182-1185 CC denotes and
T851 1186-1191 NN denotes mMGFP
T852 1192-1193 -LRB- denotes [
T853 1193-1194 CD denotes 5
T854 1194-1195 -RRB- denotes ]
T855 1195-1197 , denotes ,
T856 1197-1201 VBP denotes have
T822 1202-1208 VBN denotes gained
T857 1209-1219 NN denotes popularity
T858 1220-1223 IN denotes for
T859 1224-1227 NN denotes use
T860 1228-1230 IN denotes in
T861 1231-1235 NNS denotes mice
T862 1235-1236 . denotes .
T863 1236-1472 sentence denotes Recently however, several additional mutants of wtGFP, with altered excitation and emission spectral profiles (i.e. fluoresce in colors other than green), as well as improved thermostability and fluorescence, have been described [4,6].
T864 1237-1245 RB denotes Recently
T866 1246-1253 RB denotes however
T867 1253-1255 , denotes ,
T868 1255-1262 JJ denotes several
T870 1263-1273 JJ denotes additional
T869 1274-1281 NNS denotes mutants
T871 1282-1284 IN denotes of
T872 1285-1290 NN denotes wtGFP
T873 1290-1292 , denotes ,
T874 1292-1296 IN denotes with
T875 1297-1304 VBN denotes altered
T877 1305-1315 NN denotes excitation
T878 1316-1319 CC denotes and
T879 1320-1328 NN denotes emission
T880 1329-1337 JJ denotes spectral
T876 1338-1346 NNS denotes profiles
T881 1347-1348 -LRB- denotes (
T883 1348-1352 FW denotes i.e.
T882 1353-1362 VB denotes fluoresce
T884 1363-1365 IN denotes in
T885 1366-1372 NNS denotes colors
T886 1373-1378 JJ denotes other
T887 1379-1383 IN denotes than
T888 1384-1389 JJ denotes green
T889 1389-1390 -RRB- denotes )
T890 1390-1392 , denotes ,
T891 1392-1394 RB denotes as
T893 1395-1399 RB denotes well
T892 1400-1402 IN denotes as
T894 1403-1411 JJ denotes improved
T895 1412-1427 NN denotes thermostability
T896 1428-1431 CC denotes and
T897 1432-1444 NN denotes fluorescence
T898 1444-1446 , denotes ,
T899 1446-1450 VBP denotes have
T900 1451-1455 VBN denotes been
T865 1456-1465 VBN denotes described
T901 1466-1467 -LRB- denotes [
T903 1467-1468 CD denotes 4
T904 1468-1469 , denotes ,
T902 1469-1470 CD denotes 6
T905 1470-1471 -RRB- denotes ]
T906 1471-1472 . denotes .
T907 1472-1612 sentence denotes Dual color imaging of several GFP variant proteins is a very attractive prospect and has become a standard procedure in cell biology [7,8].
T908 1473-1477 JJ denotes Dual
T909 1478-1483 NN denotes color
T910 1484-1491 NN denotes imaging
T912 1492-1494 IN denotes of
T913 1495-1502 JJ denotes several
T915 1503-1506 NN denotes GFP
T916 1507-1514 NN denotes variant
T914 1515-1523 NN denotes proteins
T911 1524-1526 VBZ denotes is
T917 1527-1528 DT denotes a
T919 1529-1533 RB denotes very
T920 1534-1544 JJ denotes attractive
T918 1545-1553 NN denotes prospect
T921 1554-1557 CC denotes and
T922 1558-1561 VBZ denotes has
T923 1562-1568 VBN denotes become
T924 1569-1570 DT denotes a
T926 1571-1579 JJ denotes standard
T925 1580-1589 NN denotes procedure
T927 1590-1592 IN denotes in
T928 1593-1597 NN denotes cell
T929 1598-1605 NN denotes biology
T930 1606-1607 -LRB- denotes [
T932 1607-1608 CD denotes 7
T933 1608-1609 , denotes ,
T931 1609-1610 CD denotes 8
T934 1610-1611 -RRB- denotes ]
T935 1611-1612 . denotes .
T936 1612-1787 sentence denotes It has also recently been demonstrated in a subset of cells within the central nervous system (CNS) of mice using both transgenic and viral infection-based approaches [9,10].
T937 1613-1615 PRP denotes It
T939 1616-1619 VBZ denotes has
T940 1620-1624 RB denotes also
T941 1625-1633 RB denotes recently
T942 1634-1638 VBN denotes been
T938 1639-1651 VBN denotes demonstrated
T943 1652-1654 IN denotes in
T944 1655-1656 DT denotes a
T945 1657-1663 NN denotes subset
T946 1664-1666 IN denotes of
T947 1667-1672 NNS denotes cells
T948 1673-1679 IN denotes within
T949 1680-1683 DT denotes the
T951 1684-1691 JJ denotes central
T952 1692-1699 JJ denotes nervous
T950 1700-1706 NN denotes system
T953 1707-1708 -LRB- denotes (
T954 1708-1711 NN denotes CNS
T955 1711-1712 -RRB- denotes )
T956 1713-1715 IN denotes of
T957 1716-1720 NNS denotes mice
T958 1721-1726 VBG denotes using
T959 1727-1731 CC denotes both
T960 1732-1742 JJ denotes transgenic
T962 1743-1746 CC denotes and
T963 1747-1752 JJ denotes viral
T964 1753-1762 JJ denotes infection
T966 1762-1763 HYPH denotes -
T965 1763-1768 JJ denotes based
T961 1769-1779 NNS denotes approaches
T967 1780-1781 -LRB- denotes [
T969 1781-1782 CD denotes 9
T970 1782-1783 , denotes ,
T968 1783-1785 CD denotes 10
T971 1785-1786 -RRB- denotes ]
T972 1786-1787 . denotes .
T973 1787-2036 sentence denotes We therefore wished to investigate the feasibility of non-invasive multiple reporter imaging in mice, and as a first step we wanted to ascertain whether we could establish viable and fertile mice with widespread expression of GFP variant reporters.
T974 1788-1790 PRP denotes We
T976 1791-1800 RB denotes therefore
T975 1801-1807 VBD denotes wished
T977 1808-1810 TO denotes to
T978 1811-1822 VB denotes investigate
T979 1823-1826 DT denotes the
T980 1827-1838 NN denotes feasibility
T981 1839-1841 IN denotes of
T982 1842-1854 JJ denotes non-invasive
T984 1855-1863 JJ denotes multiple
T985 1864-1872 NN denotes reporter
T983 1873-1880 NN denotes imaging
T986 1881-1883 IN denotes in
T987 1884-1888 NNS denotes mice
T988 1888-1890 , denotes ,
T989 1890-1893 CC denotes and
T990 1894-1896 IN denotes as
T992 1897-1898 DT denotes a
T994 1899-1904 JJ denotes first
T993 1905-1909 NN denotes step
T995 1910-1912 PRP denotes we
T991 1913-1919 VBD denotes wanted
T996 1920-1922 TO denotes to
T997 1923-1932 VB denotes ascertain
T998 1933-1940 IN denotes whether
T1000 1941-1943 PRP denotes we
T1001 1944-1949 MD denotes could
T999 1950-1959 VB denotes establish
T1002 1960-1966 JJ denotes viable
T1004 1967-1970 CC denotes and
T1005 1971-1978 JJ denotes fertile
T1003 1979-1983 NNS denotes mice
T1006 1984-1988 IN denotes with
T1007 1989-1999 JJ denotes widespread
T1008 2000-2010 NN denotes expression
T1009 2011-2013 IN denotes of
T1010 2014-2017 NN denotes GFP
T1011 2018-2025 NN denotes variant
T1012 2026-2035 NNS denotes reporters
T1013 2035-2036 . denotes .
R354 T646 T647 det The,ease
R355 T647 T648 nsubj ease,establish
R356 T649 T647 prep of,ease
R357 T650 T649 pcomp manipulating,of
R358 T651 T652 poss its,genome
R359 T652 T650 dobj genome,manipulating
R360 T653 T648 aux has,establish
R361 T654 T648 aux helped,establish
R362 T655 T656 det the,mouse
R363 T656 T648 dobj mouse,establish
R364 T657 T648 prep as,establish
R365 T658 T659 det the,organism
R366 T659 T657 pobj organism,as
R367 T660 T659 amod premier,organism
R368 T661 T659 compound model,organism
R369 T662 T659 prep for,organism
R370 T663 T664 amod mammalian,studies
R371 T664 T662 pobj studies,for
R372 T665 T664 amod genetic,studies
R373 T666 T648 punct .,establish
R374 T668 T669 preconj Both,directed
R375 T669 T670 amod directed,approaches
R376 T670 T674 nsubj approaches,become
R377 T671 T669 cc and,directed
R378 T672 T669 conj random,directed
R379 T673 T670 compound mutagenesis,approaches
R380 T675 T670 punct ", ",approaches
R381 T676 T670 prep including,approaches
R382 T677 T678 det the,technologies
R383 T678 T676 pobj technologies,including
R384 T679 T678 prep of,technologies
R385 T680 T679 pobj transgenesis,of
R386 T681 T680 cc and,transgenesis
R387 T682 T683 compound gene,targeting
R388 T683 T680 conj targeting,transgenesis
R389 T684 T683 prep in,targeting
R390 T685 T686 compound ES,cells
R391 T686 T684 pobj cells,in
R392 T687 T674 punct ", ",become
R393 T688 T674 aux have,become
R394 T689 T674 acomp commonplace,become
R395 T690 T674 punct .,become
R396 T692 T693 advmod Genetically,manipulated
R397 T693 T694 amod manipulated,mice
R398 T694 T695 nsubjpass mice,used
R399 T696 T694 punct ", ",mice
R400 T697 T698 advmod often,incorporating
R401 T698 T694 acl incorporating,mice
R402 T699 T700 npadvmod gene,based
R403 T700 T702 amod based,reporters
R404 T701 T700 punct -,based
R405 T702 T698 dobj reporters,incorporating
R406 T703 T695 punct ", ",used
R407 T704 T695 aux are,used
R408 T705 T695 advmod frequently,used
R409 T706 T695 auxpass being,used
R410 T707 T708 aux to,model
R411 T708 T695 advcl model,used
R412 T709 T708 cc and,model
R413 T710 T708 conj understand,model
R414 T711 T712 amod mammalian,development
R415 T712 T710 dobj development,understand
R416 T713 T712 cc and,development
R417 T714 T715 compound disease,processes
R418 T715 T712 conj processes,development
R419 T716 T695 punct .,used
R420 T718 T719 amod Fluorescent,protein
R421 T719 T720 compound protein,reporters
R422 T720 T721 nsubj reporters,represent
R423 T722 T721 advmod currently,represent
R424 T723 T724 det a,alternative
R425 T724 T721 dobj alternative,represent
R426 T725 T724 amod superior,alternative
R427 T726 T724 prep to,alternative
R428 T727 T728 amod other,reporters
R429 T728 T726 pobj reporters,to
R430 T729 T730 npadvmod gene,based
R431 T730 T728 amod based,reporters
R432 T731 T730 punct -,based
R433 T732 T733 amod such,as
R434 T733 T728 prep as,reporters
R435 T734 T735 det the,lacZ
R436 T735 T733 pobj lacZ,as
R437 T736 T735 amod bacterial,lacZ
R438 T737 T735 cc or,lacZ
R439 T738 T739 amod human,placental
R440 T739 T740 amod placental,phosphatase
R441 T740 T735 conj phosphatase,lacZ
R442 T741 T740 amod alkaline,phosphatase
R443 T742 T743 mark in,is
R444 T743 T721 advcl is,represent
R445 T744 T743 mark that,is
R446 T745 T746 poss their,visualization
R447 T746 T743 nsubj visualization,is
R448 T747 T743 acomp non-invasive,is
R449 T748 T743 punct ", ",is
R450 T749 T743 cc and,is
R451 T750 T751 prep as,require
R452 T751 T743 conj require,is
R453 T752 T750 amod such,as
R454 T753 T751 aux does,require
R455 T754 T751 neg not,require
R456 T755 T756 amod chromogenic,substrates
R457 T756 T751 dobj substrates,require
R458 T757 T721 punct .,represent
R459 T759 T760 nsubjpass Fluorescence,monitored
R460 T761 T760 aux can,monitored
R461 T762 T760 auxpass be,monitored
R462 T763 T760 prep in,monitored
R463 T764 T765 amod real,time
R464 T765 T763 pobj time,in
R465 T766 T765 punct -,time
R466 T767 T768 advmod in,vivo
R467 T768 T760 advmod vivo,monitored
R468 T769 T768 cc and,vivo
R469 T770 T771 advmod in,situ
R470 T771 T768 conj situ,vivo
R471 T772 T760 punct ", ",monitored
R472 T773 T760 cc and,monitored
R473 T774 T760 conj has,monitored
R474 T775 T776 det the,advantage
R475 T776 T774 dobj advantage,has
R476 T777 T776 amod added,advantage
R477 T778 T779 mark in,quantified
R478 T779 T774 advcl quantified,has
R479 T780 T779 mark that,quantified
R480 T781 T779 nsubjpass it,quantified
R481 T782 T779 aux can,quantified
R482 T783 T779 auxpass be,quantified
R483 T784 T760 punct .,monitored
R484 T786 T787 det The,reporter
R485 T787 T791 nsubj reporter,is
R486 T788 T787 nmod prototype,reporter
R487 T789 T790 amod fluorescent,protein
R488 T790 T787 compound protein,reporter
R489 T792 T793 amod green,protein
R490 T793 T791 attr protein,is
R491 T794 T793 amod fluorescent,protein
R492 T795 T793 punct (,protein
R493 T796 T793 appos GFP,protein
R494 T797 T793 punct ", ",protein
R495 T798 T793 acl reviewed,protein
R496 T799 T798 prep in,reviewed
R497 T800 T799 punct [,in
R498 T801 T799 pobj 3,in
R499 T802 T799 punct ],in
R500 T803 T793 punct ),protein
R501 T804 T793 punct ", ",protein
R502 T805 T806 dep which,derived
R503 T806 T793 relcl derived,protein
R504 T807 T806 auxpass is,derived
R505 T808 T806 prep from,derived
R506 T809 T810 det the,jellyfish
R507 T810 T808 pobj jellyfish,from
R508 T811 T810 amod bioluminescent,jellyfish
R509 T812 T813 compound Aequorea,Victoria
R510 T813 T810 appos Victoria,jellyfish
R511 T814 T815 punct [,1
R512 T815 T791 parataxis 1,is
R513 T816 T815 punct ],1
R514 T817 T791 punct .,is
R515 T819 T820 amod Green,variants
R516 T820 T822 nsubj variants,gained
R517 T821 T820 amod fluorescent,variants
R518 T823 T820 prep of,variants
R519 T824 T825 amod wild,type
R520 T825 T826 compound type,GFP
R521 T826 T823 pobj GFP,of
R522 T827 T826 punct (,GFP
R523 T828 T826 appos wtGFP,GFP
R524 T829 T820 punct ),variants
R525 T830 T820 prep with,variants
R526 T831 T832 amod improved,thermostability
R527 T832 T830 pobj thermostability,with
R528 T833 T832 cc and,thermostability
R529 T834 T835 compound fluorescence,emission
R530 T835 T832 conj emission,thermostability
R531 T836 T820 punct ", ",variants
R532 T837 T820 prep including,variants
R533 T838 T839 amod enhanced,protein
R534 T839 T837 pobj protein,including
R535 T840 T839 amod green,protein
R536 T841 T839 amod fluorescent,protein
R537 T842 T839 punct (,protein
R538 T843 T839 appos EGFP,protein
R539 T844 T839 punct ", ",protein
R540 T845 T846 punct [,4
R541 T846 T839 parataxis 4,protein
R542 T847 T846 punct ],4
R543 T848 T839 punct ),protein
R544 T849 T839 punct ", ",protein
R545 T850 T839 cc and,protein
R546 T851 T839 conj mMGFP,protein
R547 T852 T853 punct [,5
R548 T853 T851 parataxis 5,mMGFP
R549 T854 T853 punct ],5
R550 T855 T822 punct ", ",gained
R551 T856 T822 aux have,gained
R552 T857 T822 dobj popularity,gained
R553 T858 T822 prep for,gained
R554 T859 T858 pobj use,for
R555 T860 T859 prep in,use
R556 T861 T860 pobj mice,in
R557 T862 T822 punct .,gained
R558 T864 T865 advmod Recently,described
R559 T866 T865 advmod however,described
R560 T867 T865 punct ", ",described
R561 T868 T869 amod several,mutants
R562 T869 T865 nsubjpass mutants,described
R563 T870 T869 amod additional,mutants
R564 T871 T869 prep of,mutants
R565 T872 T871 pobj wtGFP,of
R566 T873 T869 punct ", ",mutants
R567 T874 T869 prep with,mutants
R568 T875 T876 amod altered,profiles
R569 T876 T874 pobj profiles,with
R570 T877 T876 nmod excitation,profiles
R571 T878 T877 cc and,excitation
R572 T879 T877 conj emission,excitation
R573 T880 T876 amod spectral,profiles
R574 T881 T882 punct (,fluoresce
R575 T882 T876 parataxis fluoresce,profiles
R576 T883 T882 advmod i.e.,fluoresce
R577 T884 T882 prep in,fluoresce
R578 T885 T884 pobj colors,in
R579 T886 T887 amod other,than
R580 T887 T885 prep than,colors
R581 T888 T887 pobj green,than
R582 T889 T882 punct ),fluoresce
R583 T890 T876 punct ", ",profiles
R584 T891 T892 advmod as,as
R585 T892 T876 cc as,profiles
R586 T893 T892 advmod well,as
R587 T894 T895 amod improved,thermostability
R588 T895 T876 conj thermostability,profiles
R589 T896 T895 cc and,thermostability
R590 T897 T895 conj fluorescence,thermostability
R591 T898 T865 punct ", ",described
R592 T899 T865 aux have,described
R593 T900 T865 auxpass been,described
R594 T901 T902 punct [,6
R595 T902 T865 parataxis 6,described
R596 T903 T902 nummod 4,6
R597 T904 T902 punct ",",6
R598 T905 T902 punct ],6
R599 T906 T865 punct .,described
R600 T908 T909 amod Dual,color
R601 T909 T910 compound color,imaging
R602 T910 T911 nsubj imaging,is
R603 T912 T910 prep of,imaging
R604 T913 T914 amod several,proteins
R605 T914 T912 pobj proteins,of
R606 T915 T916 compound GFP,variant
R607 T916 T914 compound variant,proteins
R608 T917 T918 det a,prospect
R609 T918 T911 attr prospect,is
R610 T919 T920 advmod very,attractive
R611 T920 T918 amod attractive,prospect
R612 T921 T911 cc and,is
R613 T922 T923 aux has,become
R614 T923 T911 conj become,is
R615 T924 T925 det a,procedure
R616 T925 T923 attr procedure,become
R617 T926 T925 amod standard,procedure
R618 T927 T925 prep in,procedure
R619 T928 T929 compound cell,biology
R620 T929 T927 pobj biology,in
R621 T930 T931 punct [,8
R622 T931 T923 parataxis 8,become
R623 T932 T931 nummod 7,8
R624 T933 T931 punct ",",8
R625 T934 T931 punct ],8
R626 T935 T911 punct .,is
R627 T937 T938 nsubjpass It,demonstrated
R628 T939 T938 aux has,demonstrated
R629 T940 T938 advmod also,demonstrated
R630 T941 T938 advmod recently,demonstrated
R631 T942 T938 auxpass been,demonstrated
R632 T943 T938 prep in,demonstrated
R633 T944 T945 det a,subset
R634 T945 T943 pobj subset,in
R635 T946 T945 prep of,subset
R636 T947 T946 pobj cells,of
R637 T948 T945 prep within,subset
R638 T949 T950 det the,system
R639 T950 T948 pobj system,within
R640 T951 T950 amod central,system
R641 T952 T950 amod nervous,system
R642 T953 T950 punct (,system
R643 T954 T950 appos CNS,system
R644 T955 T950 punct ),system
R645 T956 T950 prep of,system
R646 T957 T956 pobj mice,of
R647 T958 T938 advcl using,demonstrated
R648 T959 T960 preconj both,transgenic
R649 T960 T961 amod transgenic,approaches
R650 T961 T958 dobj approaches,using
R651 T962 T960 cc and,transgenic
R652 T963 T964 amod viral,infection
R653 T964 T965 amod infection,based
R654 T965 T960 conj based,transgenic
R655 T966 T965 punct -,based
R656 T967 T968 punct [,10
R657 T968 T938 parataxis 10,demonstrated
R658 T969 T968 nummod 9,10
R659 T970 T968 punct ",",10
R660 T971 T968 punct ],10
R661 T972 T938 punct .,demonstrated
R662 T974 T975 nsubj We,wished
R663 T976 T975 advmod therefore,wished
R664 T977 T978 aux to,investigate
R665 T978 T975 xcomp investigate,wished
R666 T979 T980 det the,feasibility
R667 T980 T978 dobj feasibility,investigate
R668 T981 T980 prep of,feasibility
R669 T982 T983 amod non-invasive,imaging
R670 T983 T981 pobj imaging,of
R671 T984 T985 amod multiple,reporter
R672 T985 T983 compound reporter,imaging
R673 T986 T983 prep in,imaging
R674 T987 T986 pobj mice,in
R675 T988 T975 punct ", ",wished
R676 T989 T975 cc and,wished
R677 T990 T991 prep as,wanted
R678 T991 T975 conj wanted,wished
R679 T992 T993 det a,step
R680 T993 T990 pobj step,as
R681 T994 T993 amod first,step
R682 T995 T991 nsubj we,wanted
R683 T996 T997 aux to,ascertain
R684 T997 T991 xcomp ascertain,wanted
R685 T998 T999 mark whether,establish
R686 T999 T997 ccomp establish,ascertain
R687 T1000 T999 nsubj we,establish
R688 T1001 T999 aux could,establish
R689 T1002 T1003 amod viable,mice
R690 T1003 T999 dobj mice,establish
R691 T1004 T1002 cc and,viable
R692 T1005 T1002 conj fertile,viable
R693 T1006 T999 prep with,establish
R694 T1007 T1008 amod widespread,expression
R695 T1008 T1006 pobj expression,with
R696 T1009 T1008 prep of,expression
R697 T1010 T1011 compound GFP,variant
R698 T1011 T1012 compound variant,reporters
R699 T1012 T1009 pobj reporters,of
R700 T1013 T991 punct .,wanted