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PMC:116589 / 19661-20422 JSONTXT 6 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T3780 0-6 NN denotes Tissue
T3781 7-14 NN denotes culture
T3782 14-135 sentence denotes R1 ES cells [20] were maintained under standard culture conditions in the presence of LIF, as described previously [21].
T3783 15-17 NN denotes R1
T3785 18-20 NN denotes ES
T3784 21-26 NNS denotes cells
T3787 27-28 -LRB- denotes [
T3788 28-30 CD denotes 20
T3789 30-31 -RRB- denotes ]
T3790 32-36 VBD denotes were
T3786 37-47 VBN denotes maintained
T3791 48-53 IN denotes under
T3792 54-62 JJ denotes standard
T3794 63-70 NN denotes culture
T3793 71-81 NNS denotes conditions
T3795 82-84 IN denotes in
T3796 85-88 DT denotes the
T3797 89-97 NN denotes presence
T3798 98-100 IN denotes of
T3799 101-104 NN denotes LIF
T3800 104-106 , denotes ,
T3801 106-108 IN denotes as
T3802 109-118 VBN denotes described
T3803 119-129 RB denotes previously
T3804 130-131 -LRB- denotes [
T3805 131-133 CD denotes 21
T3806 133-134 -RRB- denotes ]
T3807 134-135 . denotes .
T3808 135-274 sentence denotes 20 μg of SalI linearized pCX-ECFP, pCX-EYFP or pCX-DsRed1 was co-electroporated into 2 × 107 cells with 5–10 μg of circular pPGKPuro [22].
T3809 136-138 CD denotes 20
T3810 139-141 NNS denotes μg
T3812 142-144 IN denotes of
T3813 145-149 NN denotes SalI
T3814 150-160 VBN denotes linearized
T3816 161-164 NN denotes pCX
T3817 164-165 HYPH denotes -
T3815 165-169 NN denotes ECFP
T3818 169-171 , denotes ,
T3819 171-174 NN denotes pCX
T3821 174-175 HYPH denotes -
T3820 175-179 NN denotes EYFP
T3822 180-182 CC denotes or
T3823 183-186 NN denotes pCX
T3825 186-187 HYPH denotes -
T3824 187-193 NN denotes DsRed1
T3826 194-197 VBD denotes was
T3811 198-215 VBN denotes co-electroporated
T3827 216-220 IN denotes into
T3828 221-222 CD denotes 2
T3830 223-224 SYM denotes ×
T3829 225-228 CD denotes 107
T3831 229-234 NNS denotes cells
T3832 235-239 IN denotes with
T3833 240-241 CD denotes 5
T3835 241-242 SYM denotes –
T3834 242-244 CD denotes 10
T3836 245-247 NNS denotes μg
T3837 248-250 IN denotes of
T3838 251-259 JJ denotes circular
T3839 260-268 NN denotes pPGKPuro
T3840 269-270 -LRB- denotes [
T3841 270-272 CD denotes 22
T3842 272-273 -RRB- denotes ]
T3843 273-274 . denotes .
T3844 274-384 sentence denotes This provided transient drug resistance which could be used as a selection for obtaining individual colonies.
T3845 275-279 DT denotes This
T3846 280-288 VBN denotes provided
T3847 289-298 JJ denotes transient
T3849 299-303 NN denotes drug
T3848 304-314 NN denotes resistance
T3850 315-320 WDT denotes which
T3852 321-326 MD denotes could
T3853 327-329 VB denotes be
T3851 330-334 VBN denotes used
T3854 335-337 IN denotes as
T3855 338-339 DT denotes a
T3856 340-349 NN denotes selection
T3857 350-353 IN denotes for
T3858 354-363 VBG denotes obtaining
T3859 364-374 JJ denotes individual
T3860 375-383 NNS denotes colonies
T3861 383-384 . denotes .
T3862 384-529 sentence denotes Selection was applied 24 hours after electroporation and maintained for 5 days, thereafter cells were propagated in in the absence of selection.
T3863 385-394 NN denotes Selection
T3865 395-398 VBD denotes was
T3864 399-406 VBN denotes applied
T3867 407-409 CD denotes 24
T3868 410-415 NNS denotes hours
T3869 416-421 IN denotes after
T3870 422-437 NN denotes electroporation
T3871 438-441 CC denotes and
T3872 442-452 VBN denotes maintained
T3873 453-456 IN denotes for
T3874 457-458 CD denotes 5
T3875 459-463 NNS denotes days
T3876 463-465 , denotes ,
T3877 465-475 RB denotes thereafter
T3878 476-481 NNS denotes cells
T3879 482-486 VBD denotes were
T3866 487-497 VBN denotes propagated
T3880 498-500 RP denotes in
T3881 501-503 IN denotes in
T3882 504-507 DT denotes the
T3883 508-515 NN denotes absence
T3884 516-518 IN denotes of
T3885 519-528 NN denotes selection
T3886 528-529 . denotes .
T3887 529-635 sentence denotes Fluorescent colonies were picked into 96-well plates, expanded and archived as described previously ([2].
T3888 530-541 JJ denotes Fluorescent
T3889 542-550 NNS denotes colonies
T3891 551-555 VBD denotes were
T3890 556-562 VBN denotes picked
T3892 563-567 IN denotes into
T3893 568-570 CD denotes 96
T3895 570-571 HYPH denotes -
T3894 571-575 NN denotes well
T3896 576-582 NNS denotes plates
T3897 582-584 , denotes ,
T3898 584-592 VBD denotes expanded
T3899 593-596 CC denotes and
T3900 597-605 VBD denotes archived
T3901 606-608 IN denotes as
T3902 609-618 VBN denotes described
T3903 619-629 RB denotes previously
T3904 630-631 -LRB- denotes (
T3905 631-632 -LRB- denotes [
T3906 632-633 CD denotes 2
T3907 633-634 -RRB- denotes ]
T3908 634-635 . denotes .
T3909 635-761 sentence denotes Clones were screened for strong, stable, and homogenous transgene expression during culture in the absence of drug selection.
T3910 636-642 NNS denotes Clones
T3912 643-647 VBD denotes were
T3911 648-656 VBN denotes screened
T3913 657-660 IN denotes for
T3914 661-667 JJ denotes strong
T3916 667-669 , denotes ,
T3917 669-675 JJ denotes stable
T3918 675-677 , denotes ,
T3919 677-680 CC denotes and
T3920 681-691 JJ denotes homogenous
T3921 692-701 NN denotes transgene
T3915 702-712 NN denotes expression
T3922 713-719 IN denotes during
T3923 720-727 NN denotes culture
T3924 728-730 IN denotes in
T3925 731-734 DT denotes the
T3926 735-742 NN denotes absence
T3927 743-745 IN denotes of
T3928 746-750 NN denotes drug
T3929 751-760 NN denotes selection
T3930 760-761 . denotes .
R2430 T3780 T3781 compound Tissue,culture
R2431 T3783 T3784 compound R1,cells
R2432 T3784 T3786 nsubjpass cells,maintained
R2433 T3785 T3784 compound ES,cells
R2434 T3787 T3788 punct [,20
R2435 T3788 T3784 parataxis 20,cells
R2436 T3789 T3788 punct ],20
R2437 T3790 T3786 auxpass were,maintained
R2438 T3791 T3786 prep under,maintained
R2439 T3792 T3793 amod standard,conditions
R2440 T3793 T3791 pobj conditions,under
R2441 T3794 T3793 compound culture,conditions
R2442 T3795 T3786 prep in,maintained
R2443 T3796 T3797 det the,presence
R2444 T3797 T3795 pobj presence,in
R2445 T3798 T3797 prep of,presence
R2446 T3799 T3798 pobj LIF,of
R2447 T3800 T3786 punct ", ",maintained
R2448 T3801 T3802 mark as,described
R2449 T3802 T3786 advcl described,maintained
R2450 T3803 T3802 advmod previously,described
R2451 T3804 T3805 punct [,21
R2452 T3805 T3786 parataxis 21,maintained
R2453 T3806 T3805 punct ],21
R2454 T3807 T3786 punct .,maintained
R2455 T3809 T3810 nummod 20,μg
R2456 T3810 T3811 nsubjpass μg,co-electroporated
R2457 T3812 T3810 prep of,μg
R2458 T3813 T3814 npadvmod SalI,linearized
R2459 T3814 T3815 amod linearized,ECFP
R2460 T3815 T3812 pobj ECFP,of
R2461 T3816 T3815 compound pCX,ECFP
R2462 T3817 T3815 punct -,ECFP
R2463 T3818 T3815 punct ", ",ECFP
R2464 T3819 T3820 compound pCX,EYFP
R2465 T3820 T3815 conj EYFP,ECFP
R2466 T3821 T3820 punct -,EYFP
R2467 T3822 T3820 cc or,EYFP
R2468 T3823 T3824 compound pCX,DsRed1
R2469 T3824 T3820 conj DsRed1,EYFP
R2470 T3825 T3824 punct -,DsRed1
R2471 T3826 T3811 auxpass was,co-electroporated
R2472 T3827 T3811 prep into,co-electroporated
R2473 T3828 T3829 quantmod 2,107
R2474 T3829 T3831 nummod 107,cells
R2475 T3830 T3829 punct ×,107
R2476 T3831 T3827 pobj cells,into
R2477 T3832 T3811 prep with,co-electroporated
R2478 T3833 T3834 quantmod 5,10
R2479 T3834 T3836 nummod 10,μg
R2480 T3835 T3834 punct –,10
R2481 T3836 T3832 pobj μg,with
R2482 T3837 T3836 prep of,μg
R2483 T3838 T3839 amod circular,pPGKPuro
R2484 T3839 T3837 pobj pPGKPuro,of
R2485 T3840 T3841 punct [,22
R2486 T3841 T3811 parataxis 22,co-electroporated
R2487 T3842 T3841 punct ],22
R2488 T3843 T3811 punct .,co-electroporated
R2489 T3845 T3846 nsubj This,provided
R2490 T3847 T3848 amod transient,resistance
R2491 T3848 T3846 dobj resistance,provided
R2492 T3849 T3848 compound drug,resistance
R2493 T3850 T3851 dep which,used
R2494 T3851 T3848 relcl used,resistance
R2495 T3852 T3851 aux could,used
R2496 T3853 T3851 auxpass be,used
R2497 T3854 T3851 prep as,used
R2498 T3855 T3856 det a,selection
R2499 T3856 T3854 pobj selection,as
R2500 T3857 T3856 prep for,selection
R2501 T3858 T3857 pcomp obtaining,for
R2502 T3859 T3860 amod individual,colonies
R2503 T3860 T3858 dobj colonies,obtaining
R2504 T3861 T3846 punct .,provided
R2505 T3863 T3864 nsubjpass Selection,applied
R2506 T3864 T3866 ccomp applied,propagated
R2507 T3865 T3864 auxpass was,applied
R2508 T3867 T3868 nummod 24,hours
R2509 T3868 T3869 npadvmod hours,after
R2510 T3869 T3864 prep after,applied
R2511 T3870 T3869 pobj electroporation,after
R2512 T3871 T3864 cc and,applied
R2513 T3872 T3864 conj maintained,applied
R2514 T3873 T3872 prep for,maintained
R2515 T3874 T3875 nummod 5,days
R2516 T3875 T3873 pobj days,for
R2517 T3876 T3866 punct ", ",propagated
R2518 T3877 T3866 advmod thereafter,propagated
R2519 T3878 T3866 nsubjpass cells,propagated
R2520 T3879 T3866 auxpass were,propagated
R2521 T3880 T3866 prt in,propagated
R2522 T3881 T3866 prep in,propagated
R2523 T3882 T3883 det the,absence
R2524 T3883 T3881 pobj absence,in
R2525 T3884 T3883 prep of,absence
R2526 T3885 T3884 pobj selection,of
R2527 T3886 T3866 punct .,propagated
R2528 T3888 T3889 amod Fluorescent,colonies
R2529 T3889 T3890 nsubj colonies,picked
R2530 T3891 T3890 aux were,picked
R2531 T3892 T3890 prep into,picked
R2532 T3893 T3894 nummod 96,well
R2533 T3894 T3896 compound well,plates
R2534 T3895 T3894 punct -,well
R2535 T3896 T3892 pobj plates,into
R2536 T3897 T3890 punct ", ",picked
R2537 T3898 T3890 conj expanded,picked
R2538 T3899 T3898 cc and,expanded
R2539 T3900 T3898 conj archived,expanded
R2540 T3901 T3902 mark as,described
R2541 T3902 T3890 advcl described,picked
R2542 T3903 T3902 advmod previously,described
R2543 T3904 T3902 punct (,described
R2544 T3905 T3906 punct [,2
R2545 T3906 T3902 parataxis 2,described
R2546 T3907 T3906 punct ],2
R2547 T3908 T3890 punct .,picked
R2548 T3910 T3911 nsubjpass Clones,screened
R2549 T3912 T3911 auxpass were,screened
R2550 T3913 T3911 prep for,screened
R2551 T3914 T3915 amod strong,expression
R2552 T3915 T3913 pobj expression,for
R2553 T3916 T3914 punct ", ",strong
R2554 T3917 T3914 conj stable,strong
R2555 T3918 T3917 punct ", ",stable
R2556 T3919 T3917 cc and,stable
R2557 T3920 T3917 conj homogenous,stable
R2558 T3921 T3915 compound transgene,expression
R2559 T3922 T3911 prep during,screened
R2560 T3923 T3922 pobj culture,during
R2561 T3924 T3911 prep in,screened
R2562 T3925 T3926 det the,absence
R2563 T3926 T3924 pobj absence,in
R2564 T3927 T3926 prep of,absence
R2565 T3928 T3929 compound drug,selection
R2566 T3929 T3927 pobj selection,of
R2567 T3930 T3911 punct .,screened