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PMC:116589 / 16479-17119 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T2695 0-4 IN denotes From
T2697 5-10 DT denotes these
T2698 11-23 NNS denotes observations
T2699 24-26 PRP denotes we
T2696 27-35 VBP denotes conclude
T2700 36-40 IN denotes that
T2702 41-44 DT denotes the
T2704 45-49 JJ denotes blue
T2706 49-50 HYPH denotes -
T2705 50-57 VBN denotes shifted
T2703 58-62 NN denotes ECFP
T2707 62-64 , denotes ,
T2708 64-67 CC denotes and
T2709 68-71 DT denotes the
T2711 72-75 JJ denotes red
T2713 75-76 HYPH denotes -
T2712 76-83 VBN denotes shifted
T2710 84-88 NN denotes EYFP
T2714 89-97 NNS denotes variants
T2715 98-100 IN denotes of
T2716 101-106 NN denotes wtGFP
T2701 107-110 VBP denotes are
T2717 111-115 RB denotes both
T2718 116-124 JJ denotes amenable
T2719 125-127 IN denotes to
T2720 128-131 NN denotes use
T2721 132-134 IN denotes in
T2722 135-139 NNS denotes mice
T2723 139-140 . denotes .
T2724 140-321 sentence denotes Also since we have established lines of viable and fertile mice having widespread FP expression we have demonstrated that ECFP and EYFP represent developmentally neutral reporters.
T2725 141-145 RB denotes Also
T2727 146-151 IN denotes since
T2729 152-154 PRP denotes we
T2730 155-159 VBP denotes have
T2728 160-171 VBN denotes established
T2731 172-177 NNS denotes lines
T2732 178-180 IN denotes of
T2733 181-187 JJ denotes viable
T2735 188-191 CC denotes and
T2736 192-199 JJ denotes fertile
T2734 200-204 NNS denotes mice
T2737 205-211 VBG denotes having
T2738 212-222 JJ denotes widespread
T2740 223-225 NN denotes FP
T2739 226-236 NN denotes expression
T2741 237-239 PRP denotes we
T2742 240-244 VBP denotes have
T2726 245-257 VBN denotes demonstrated
T2743 258-262 IN denotes that
T2745 263-267 NN denotes ECFP
T2746 268-271 CC denotes and
T2747 272-276 NN denotes EYFP
T2744 277-286 VBP denotes represent
T2748 287-302 RB denotes developmentally
T2749 303-310 JJ denotes neutral
T2750 311-320 NNS denotes reporters
T2751 320-321 . denotes .
T2752 321-640 sentence denotes Since we were unable to obtain ES cells, embryos or mice with widespread expression of a RFP (DsRed1) we conclude that this reporter is not developmentally neutral or that its expression cannot be sustained at levels high enough for detection, and as such is not as versatile as other gene-based fluorescent reporters.
T2753 322-327 IN denotes Since
T2755 328-330 PRP denotes we
T2754 331-335 VBD denotes were
T2757 336-342 JJ denotes unable
T2758 343-345 TO denotes to
T2759 346-352 VB denotes obtain
T2760 353-355 NN denotes ES
T2761 356-361 NNS denotes cells
T2762 361-363 , denotes ,
T2763 363-370 NNS denotes embryos
T2764 371-373 CC denotes or
T2765 374-378 NNS denotes mice
T2766 379-383 IN denotes with
T2767 384-394 JJ denotes widespread
T2768 395-405 NN denotes expression
T2769 406-408 IN denotes of
T2770 409-410 DT denotes a
T2771 411-414 NN denotes RFP
T2772 415-416 -LRB- denotes (
T2773 416-422 NN denotes DsRed1
T2774 422-423 -RRB- denotes )
T2775 424-426 PRP denotes we
T2756 427-435 VBP denotes conclude
T2776 436-440 IN denotes that
T2778 441-445 DT denotes this
T2779 446-454 NN denotes reporter
T2777 455-457 VBZ denotes is
T2780 458-461 RB denotes not
T2781 462-477 RB denotes developmentally
T2782 478-485 JJ denotes neutral
T2783 486-488 CC denotes or
T2784 489-493 IN denotes that
T2786 494-497 PRP$ denotes its
T2787 498-508 NN denotes expression
T2788 509-512 MD denotes can
T2789 512-515 RB denotes not
T2790 516-518 VB denotes be
T2785 519-528 VBN denotes sustained
T2791 529-531 IN denotes at
T2792 532-538 NNS denotes levels
T2793 539-543 JJ denotes high
T2794 544-550 JJ denotes enough
T2795 551-554 IN denotes for
T2796 555-564 NN denotes detection
T2797 564-566 , denotes ,
T2798 566-569 CC denotes and
T2799 570-572 IN denotes as
T2801 573-577 JJ denotes such
T2800 578-580 VBZ denotes is
T2802 581-584 RB denotes not
T2803 585-587 RB denotes as
T2804 588-597 JJ denotes versatile
T2805 598-600 IN denotes as
T2806 601-606 JJ denotes other
T2808 607-611 NN denotes gene
T2810 611-612 HYPH denotes -
T2809 612-617 VBN denotes based
T2811 618-629 JJ denotes fluorescent
T2807 630-639 NNS denotes reporters
T2812 639-640 . denotes .
R1849 T2695 T2696 prep From,conclude
R1850 T2697 T2698 det these,observations
R1851 T2698 T2695 pobj observations,From
R1852 T2699 T2696 nsubj we,conclude
R1853 T2700 T2701 mark that,are
R1854 T2701 T2696 ccomp are,conclude
R1855 T2702 T2703 det the,ECFP
R1856 T2703 T2701 nsubj ECFP,are
R1857 T2704 T2705 amod blue,shifted
R1858 T2705 T2703 amod shifted,ECFP
R1859 T2706 T2705 punct -,shifted
R1860 T2707 T2703 punct ", ",ECFP
R1861 T2708 T2703 cc and,ECFP
R1862 T2709 T2710 det the,EYFP
R1863 T2710 T2714 compound EYFP,variants
R1864 T2711 T2712 amod red,shifted
R1865 T2712 T2710 amod shifted,EYFP
R1866 T2713 T2712 punct -,shifted
R1867 T2714 T2703 conj variants,ECFP
R1868 T2715 T2703 prep of,ECFP
R1869 T2716 T2715 pobj wtGFP,of
R1870 T2717 T2701 advmod both,are
R1871 T2718 T2701 acomp amenable,are
R1872 T2719 T2718 prep to,amenable
R1873 T2720 T2719 pobj use,to
R1874 T2721 T2720 prep in,use
R1875 T2722 T2721 pobj mice,in
R1876 T2723 T2696 punct .,conclude
R1877 T2725 T2726 advmod Also,demonstrated
R1878 T2727 T2728 mark since,established
R1879 T2728 T2726 advcl established,demonstrated
R1880 T2729 T2728 nsubj we,established
R1881 T2730 T2728 aux have,established
R1882 T2731 T2728 dobj lines,established
R1883 T2732 T2731 prep of,lines
R1884 T2733 T2734 amod viable,mice
R1885 T2734 T2732 pobj mice,of
R1886 T2735 T2733 cc and,viable
R1887 T2736 T2733 conj fertile,viable
R1888 T2737 T2731 acl having,lines
R1889 T2738 T2739 amod widespread,expression
R1890 T2739 T2737 dobj expression,having
R1891 T2740 T2739 compound FP,expression
R1892 T2741 T2726 nsubj we,demonstrated
R1893 T2742 T2726 aux have,demonstrated
R1894 T2743 T2744 mark that,represent
R1895 T2744 T2726 ccomp represent,demonstrated
R1896 T2745 T2744 nsubj ECFP,represent
R1897 T2746 T2745 cc and,ECFP
R1898 T2747 T2745 conj EYFP,ECFP
R1899 T2748 T2749 advmod developmentally,neutral
R1900 T2749 T2750 amod neutral,reporters
R1901 T2750 T2744 dobj reporters,represent
R1902 T2751 T2726 punct .,demonstrated
R1903 T2753 T2754 mark Since,were
R1904 T2754 T2756 advcl were,conclude
R1905 T2755 T2754 nsubj we,were
R1906 T2757 T2754 acomp unable,were
R1907 T2758 T2759 aux to,obtain
R1908 T2759 T2757 xcomp obtain,unable
R1909 T2760 T2761 compound ES,cells
R1910 T2761 T2759 dobj cells,obtain
R1911 T2762 T2761 punct ", ",cells
R1912 T2763 T2761 conj embryos,cells
R1913 T2764 T2763 cc or,embryos
R1914 T2765 T2763 conj mice,embryos
R1915 T2766 T2761 prep with,cells
R1916 T2767 T2768 amod widespread,expression
R1917 T2768 T2766 pobj expression,with
R1918 T2769 T2768 prep of,expression
R1919 T2770 T2771 det a,RFP
R1920 T2771 T2769 pobj RFP,of
R1921 T2772 T2771 punct (,RFP
R1922 T2773 T2771 appos DsRed1,RFP
R1923 T2774 T2756 punct ),conclude
R1924 T2775 T2756 nsubj we,conclude
R1925 T2776 T2777 mark that,is
R1926 T2777 T2756 advcl is,conclude
R1927 T2778 T2779 det this,reporter
R1928 T2779 T2777 nsubj reporter,is
R1929 T2780 T2777 neg not,is
R1930 T2781 T2782 advmod developmentally,neutral
R1931 T2782 T2777 acomp neutral,is
R1932 T2783 T2777 cc or,is
R1933 T2784 T2785 mark that,sustained
R1934 T2785 T2777 conj sustained,is
R1935 T2786 T2787 poss its,expression
R1936 T2787 T2785 nsubjpass expression,sustained
R1937 T2788 T2785 aux can,sustained
R1938 T2789 T2785 neg not,sustained
R1939 T2790 T2785 auxpass be,sustained
R1940 T2791 T2785 prep at,sustained
R1941 T2792 T2791 pobj levels,at
R1942 T2793 T2794 amod high,enough
R1943 T2794 T2792 amod enough,levels
R1944 T2795 T2794 prep for,enough
R1945 T2796 T2795 pobj detection,for
R1946 T2797 T2785 punct ", ",sustained
R1947 T2798 T2785 cc and,sustained
R1948 T2799 T2800 prep as,is
R1949 T2800 T2785 conj is,sustained
R1950 T2801 T2799 amod such,as
R1951 T2802 T2800 neg not,is
R1952 T2803 T2804 advmod as,versatile
R1953 T2804 T2800 acomp versatile,is
R1954 T2805 T2804 prep as,versatile
R1955 T2806 T2807 amod other,reporters
R1956 T2807 T2805 pobj reporters,as
R1957 T2808 T2809 npadvmod gene,based
R1958 T2809 T2807 amod based,reporters
R1959 T2810 T2809 punct -,based
R1960 T2811 T2807 amod fluorescent,reporters
R1961 T2812 T2756 punct .,conclude