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PMC:116589 / 138-2144 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T177 0-10 sentence denotes Background
T178 0-10 NN denotes Background
T179 10-122 sentence denotes Non-invasive autofluorescent reporters have revolutionized lineage labeling in an array of different organisms.
T180 11-23 JJ denotes Non-invasive
T182 24-39 JJ denotes autofluorescent
T181 40-49 NNS denotes reporters
T184 50-54 VBP denotes have
T183 55-69 VBN denotes revolutionized
T185 70-77 NN denotes lineage
T186 78-86 NN denotes labeling
T187 87-89 IN denotes in
T188 90-92 DT denotes an
T189 93-98 NN denotes array
T190 99-101 IN denotes of
T191 102-111 JJ denotes different
T192 112-121 NNS denotes organisms
T193 121-122 . denotes .
T194 122-296 sentence denotes In recent years green fluorescent protein (GFP) from the bioluminescent jellyfish Aequoria Victoria has gained popularity in mouse transgenic and gene targeting regimes [1].
T195 123-125 IN denotes In
T197 126-132 JJ denotes recent
T198 133-138 NNS denotes years
T199 139-144 JJ denotes green
T201 145-156 JJ denotes fluorescent
T200 157-164 NN denotes protein
T202 165-166 -LRB- denotes (
T203 166-169 NN denotes GFP
T204 169-170 -RRB- denotes )
T205 171-175 IN denotes from
T206 176-179 DT denotes the
T208 180-194 JJ denotes bioluminescent
T207 195-204 NN denotes jellyfish
T209 205-213 NNP denotes Aequoria
T210 214-222 NNP denotes Victoria
T211 223-226 VBZ denotes has
T196 227-233 VBN denotes gained
T212 234-244 NN denotes popularity
T213 245-247 IN denotes in
T214 248-253 NN denotes mouse
T215 254-264 JJ denotes transgenic
T217 265-268 CC denotes and
T218 269-273 NN denotes gene
T219 274-283 VBG denotes targeting
T216 284-291 NNS denotes regimes
T220 292-293 -LRB- denotes [
T221 293-294 CD denotes 1
T222 294-295 -RRB- denotes ]
T223 295-296 . denotes .
T224 296-500 sentence denotes It offers several advantages over conventional gene-based reporters, such as lacZ and alkaline phosphatase, in that its visualization does not require a chromogenic substrate and can be realized in vivo.
T225 297-299 PRP denotes It
T226 300-306 VBZ denotes offers
T227 307-314 JJ denotes several
T228 315-325 NNS denotes advantages
T229 326-330 IN denotes over
T230 331-343 JJ denotes conventional
T232 344-348 NN denotes gene
T234 348-349 HYPH denotes -
T233 349-354 VBN denotes based
T231 355-364 NNS denotes reporters
T235 364-366 , denotes ,
T236 366-370 JJ denotes such
T237 371-373 IN denotes as
T238 374-378 NN denotes lacZ
T239 379-382 CC denotes and
T240 383-391 NN denotes alkaline
T241 392-403 NN denotes phosphatase
T242 403-405 , denotes ,
T243 405-407 IN denotes in
T245 408-412 IN denotes that
T246 413-416 PRP$ denotes its
T247 417-430 NN denotes visualization
T248 431-435 VBZ denotes does
T249 436-439 RB denotes not
T244 440-447 VB denotes require
T250 448-449 DT denotes a
T252 450-461 JJ denotes chromogenic
T251 462-471 NN denotes substrate
T253 472-475 CC denotes and
T254 476-479 MD denotes can
T256 480-482 VB denotes be
T255 483-491 VBN denotes realized
T257 492-494 FW denotes in
T258 495-499 FW denotes vivo
T259 499-500 . denotes .
T260 500-661 sentence denotes We have previously demonstrated the utility and developmental neutrality of enhanced green fluorescent protein (EGFP) in embryonic stem (ES) cells and mice [2].
T261 501-503 PRP denotes We
T263 504-508 VBP denotes have
T264 509-519 RB denotes previously
T262 520-532 VBN denotes demonstrated
T265 533-536 DT denotes the
T266 537-544 NN denotes utility
T267 545-548 CC denotes and
T268 549-562 JJ denotes developmental
T269 563-573 NN denotes neutrality
T270 574-576 IN denotes of
T271 577-585 VBN denotes enhanced
T273 586-591 JJ denotes green
T274 592-603 JJ denotes fluorescent
T272 604-611 NN denotes protein
T275 612-613 -LRB- denotes (
T276 613-617 NN denotes EGFP
T277 617-618 -RRB- denotes )
T278 619-621 IN denotes in
T279 622-631 JJ denotes embryonic
T280 632-636 NN denotes stem
T282 637-638 -LRB- denotes (
T283 638-640 NN denotes ES
T284 640-641 -RRB- denotes )
T281 642-647 NNS denotes cells
T285 648-651 CC denotes and
T286 652-656 NNS denotes mice
T287 657-658 -LRB- denotes [
T288 658-659 CD denotes 2
T289 659-660 -RRB- denotes ]
T290 660-661 . denotes .
T291 661-670 sentence denotes Results
T292 663-670 NNS denotes Results
T293 670-918 sentence denotes In this study we have used embryonic stem (ES) cell-mediated transgenesis to test the enhanced cyan fluorescent protein (ECFP) and enhanced yellow fluorescent protein (EYFP), two mutant and spectrally distinct color variants of wild type (wt) GFP.
T294 671-673 IN denotes In
T296 674-678 DT denotes this
T297 679-684 NN denotes study
T298 685-687 PRP denotes we
T299 688-692 VBP denotes have
T295 693-697 VBN denotes used
T300 698-707 JJ denotes embryonic
T301 708-712 NN denotes stem
T303 713-714 -LRB- denotes (
T304 714-716 NN denotes ES
T305 716-717 -RRB- denotes )
T302 718-722 NN denotes cell
T307 722-723 HYPH denotes -
T306 723-731 VBN denotes mediated
T308 732-744 NN denotes transgenesis
T309 745-747 TO denotes to
T310 748-752 VB denotes test
T311 753-756 DT denotes the
T313 757-765 VBN denotes enhanced
T314 766-770 NN denotes cyan
T315 771-782 JJ denotes fluorescent
T312 783-790 NN denotes protein
T316 791-792 -LRB- denotes (
T317 792-796 NN denotes ECFP
T318 796-797 -RRB- denotes )
T319 798-801 CC denotes and
T320 802-810 VBN denotes enhanced
T322 811-817 JJ denotes yellow
T323 818-829 JJ denotes fluorescent
T321 830-837 NN denotes protein
T324 838-839 -LRB- denotes (
T325 839-843 NN denotes EYFP
T326 843-844 -RRB- denotes )
T327 844-846 , denotes ,
T328 846-849 CD denotes two
T330 850-856 JJ denotes mutant
T331 857-860 CC denotes and
T332 861-871 RB denotes spectrally
T333 872-880 JJ denotes distinct
T334 881-886 NN denotes color
T329 887-895 NNS denotes variants
T335 896-898 IN denotes of
T336 899-903 JJ denotes wild
T337 904-908 NN denotes type
T339 909-910 -LRB- denotes (
T340 910-912 NN denotes wt
T341 912-913 -RRB- denotes )
T338 914-917 NN denotes GFP
T342 917-918 . denotes .
T343 918-1065 sentence denotes We have also tested DsRed1, the novel red fluorescent protein reporter recently cloned from the Discostoma coral by virtue of its homology to GFP.
T344 919-921 PRP denotes We
T346 922-926 VBP denotes have
T347 927-931 RB denotes also
T345 932-938 VBN denotes tested
T348 939-945 NN denotes DsRed1
T349 945-947 , denotes ,
T350 947-950 DT denotes the
T352 951-956 JJ denotes novel
T353 957-960 JJ denotes red
T355 961-972 JJ denotes fluorescent
T354 973-980 NN denotes protein
T351 981-989 NN denotes reporter
T356 990-998 RB denotes recently
T357 999-1005 VBN denotes cloned
T358 1006-1010 IN denotes from
T359 1011-1014 DT denotes the
T361 1015-1025 NN denotes Discostoma
T360 1026-1031 NN denotes coral
T362 1032-1034 IN denotes by
T363 1035-1041 NN denotes virtue
T364 1042-1044 IN denotes of
T365 1045-1048 PRP$ denotes its
T366 1049-1057 NN denotes homology
T367 1058-1060 IN denotes to
T368 1061-1064 NN denotes GFP
T369 1064-1065 . denotes .
T370 1065-1233 sentence denotes To this end, we have established lines of ES cells together with viable and fertile mice having widespread expression of either the ECFP or EYFP GFP-variant reporters.
T371 1066-1068 IN denotes To
T373 1069-1073 DT denotes this
T374 1074-1077 NN denotes end
T375 1077-1079 , denotes ,
T376 1079-1081 PRP denotes we
T377 1082-1086 VBP denotes have
T372 1087-1098 VBN denotes established
T378 1099-1104 NNS denotes lines
T379 1105-1107 IN denotes of
T380 1108-1110 NN denotes ES
T381 1111-1116 NNS denotes cells
T382 1117-1125 RB denotes together
T383 1126-1130 IN denotes with
T384 1131-1137 JJ denotes viable
T386 1138-1141 CC denotes and
T387 1142-1149 JJ denotes fertile
T385 1150-1154 NNS denotes mice
T388 1155-1161 VBG denotes having
T389 1162-1172 JJ denotes widespread
T390 1173-1183 NN denotes expression
T391 1184-1186 IN denotes of
T392 1187-1193 CC denotes either
T394 1194-1197 DT denotes the
T395 1198-1202 NN denotes ECFP
T396 1203-1205 CC denotes or
T397 1206-1210 NN denotes EYFP
T398 1211-1214 NN denotes GFP
T400 1214-1215 HYPH denotes -
T399 1215-1222 NN denotes variant
T393 1223-1232 NNS denotes reporters
T401 1232-1233 . denotes .
T402 1233-1413 sentence denotes However, we were unable to generate equivalent DsRed1 lines, suggesting that DsRed1 is not developmentally neutral or that transgene expression cannot be sustained constitutively.
T403 1234-1241 RB denotes However
T405 1241-1243 , denotes ,
T406 1243-1245 PRP denotes we
T404 1246-1250 VBD denotes were
T407 1251-1257 JJ denotes unable
T408 1258-1260 TO denotes to
T409 1261-1269 VB denotes generate
T410 1270-1280 JJ denotes equivalent
T412 1281-1287 NN denotes DsRed1
T411 1288-1293 NNS denotes lines
T413 1293-1295 , denotes ,
T414 1295-1305 VBG denotes suggesting
T415 1306-1310 IN denotes that
T417 1311-1317 NN denotes DsRed1
T416 1318-1320 VBZ denotes is
T418 1321-1324 RB denotes not
T419 1325-1340 RB denotes developmentally
T420 1341-1348 JJ denotes neutral
T421 1349-1351 CC denotes or
T422 1352-1356 IN denotes that
T424 1357-1366 NN denotes transgene
T425 1367-1377 NN denotes expression
T426 1378-1381 MD denotes can
T427 1381-1384 RB denotes not
T428 1385-1387 VB denotes be
T423 1388-1397 VBN denotes sustained
T429 1398-1412 RB denotes constitutively
T430 1412-1413 . denotes .
T431 1413-1681 sentence denotes Balanced (diploid <-> diploid) and polarized (tetraploid <-> diploid) chimeras comprising combinations of the ECFP and EYFP ES cells and/or embryos, demonstrate that populations of cells expressing each individual reporter can be distinguished within a single animal.
T432 1414-1422 JJ denotes Balanced
T434 1423-1424 -LRB- denotes (
T436 1424-1431 JJ denotes diploid
T437 1432-1433 SYM denotes <
T438 1433-1434 HYPH denotes -
T439 1434-1435 SYM denotes >
T435 1436-1443 JJ denotes diploid
T440 1443-1444 -RRB- denotes )
T441 1445-1448 CC denotes and
T442 1449-1458 JJ denotes polarized
T443 1459-1460 -LRB- denotes (
T445 1460-1470 JJ denotes tetraploid
T446 1471-1472 SYM denotes <
T447 1472-1473 HYPH denotes -
T448 1473-1474 SYM denotes >
T444 1475-1482 JJ denotes diploid
T449 1482-1483 -RRB- denotes )
T433 1484-1492 NNS denotes chimeras
T451 1493-1503 VBG denotes comprising
T452 1504-1516 NNS denotes combinations
T453 1517-1519 IN denotes of
T454 1520-1523 DT denotes the
T455 1524-1528 NN denotes ECFP
T456 1529-1532 CC denotes and
T457 1533-1537 NN denotes EYFP
T458 1538-1540 NN denotes ES
T459 1541-1546 NNS denotes cells
T460 1547-1550 CC denotes and
T461 1550-1551 HYPH denotes /
T462 1551-1553 CC denotes or
T463 1554-1561 NNS denotes embryos
T464 1561-1563 , denotes ,
T450 1563-1574 VBP denotes demonstrate
T465 1575-1579 IN denotes that
T467 1580-1591 NNS denotes populations
T468 1592-1594 IN denotes of
T469 1595-1600 NNS denotes cells
T470 1601-1611 VBG denotes expressing
T471 1612-1616 DT denotes each
T473 1617-1627 JJ denotes individual
T472 1628-1636 NN denotes reporter
T474 1637-1640 MD denotes can
T475 1641-1643 VB denotes be
T466 1644-1657 VBN denotes distinguished
T476 1658-1664 IN denotes within
T477 1665-1666 DT denotes a
T479 1667-1673 JJ denotes single
T478 1674-1680 NN denotes animal
T480 1680-1681 . denotes .
T481 1681-1694 sentence denotes Conclusions
T482 1683-1694 NNS denotes Conclusions
T483 1694-1798 sentence denotes GFP variant reporters are unique in allowing non-invasive multi-spectral visualization in live samples.
T484 1695-1698 NN denotes GFP
T486 1699-1706 JJ denotes variant
T485 1707-1716 NNS denotes reporters
T487 1717-1720 VBP denotes are
T488 1721-1727 JJ denotes unique
T489 1728-1730 IN denotes in
T490 1731-1739 VBG denotes allowing
T491 1740-1752 JJ denotes non-invasive
T493 1753-1767 JJ denotes multi-spectral
T492 1768-1781 NN denotes visualization
T494 1782-1784 IN denotes in
T495 1785-1789 JJ denotes live
T496 1790-1797 NNS denotes samples
T497 1797-1798 . denotes .
T498 1798-2006 sentence denotes The ECFP and EYFP-expressing transgenic ES cells and mice that we have generated provide sources of cells and tissues for combinatorial, double-tagged recombination experiments, chimeras or transplantations.
T499 1799-1802 DT denotes The
T501 1803-1807 NN denotes ECFP
T503 1808-1811 CC denotes and
T504 1812-1816 NN denotes EYFP
T505 1816-1817 HYPH denotes -
T502 1817-1827 VBG denotes expressing
T506 1828-1838 JJ denotes transgenic
T507 1839-1841 NN denotes ES
T500 1842-1847 NNS denotes cells
T509 1848-1851 CC denotes and
T510 1852-1856 NNS denotes mice
T511 1857-1861 WDT denotes that
T513 1862-1864 PRP denotes we
T514 1865-1869 VBP denotes have
T512 1870-1879 VBN denotes generated
T508 1880-1887 VBP denotes provide
T515 1888-1895 NNS denotes sources
T516 1896-1898 IN denotes of
T517 1899-1904 NNS denotes cells
T518 1905-1908 CC denotes and
T519 1909-1916 NNS denotes tissues
T520 1917-1920 IN denotes for
T521 1921-1934 JJ denotes combinatorial
T523 1934-1936 , denotes ,
T524 1936-1942 JJ denotes double
T526 1942-1943 HYPH denotes -
T525 1943-1949 JJ denotes tagged
T527 1950-1963 NN denotes recombination
T522 1964-1975 NNS denotes experiments
T528 1975-1977 , denotes ,
T529 1977-1985 NNS denotes chimeras
T530 1986-1988 CC denotes or
T531 1989-2005 NNS denotes transplantations
T532 2005-2006 . denotes .
R100 T261 T262 nsubj We,demonstrated
R101 T263 T262 aux have,demonstrated
R102 T264 T262 advmod previously,demonstrated
R103 T265 T266 det the,utility
R104 T266 T262 dobj utility,demonstrated
R105 T267 T266 cc and,utility
R106 T268 T269 amod developmental,neutrality
R107 T269 T266 conj neutrality,utility
R108 T270 T266 prep of,utility
R109 T271 T272 amod enhanced,protein
R110 T272 T270 pobj protein,of
R111 T273 T272 amod green,protein
R112 T274 T272 amod fluorescent,protein
R113 T275 T272 punct (,protein
R114 T276 T272 appos EGFP,protein
R115 T277 T266 punct ),utility
R116 T278 T266 prep in,utility
R117 T279 T280 amod embryonic,stem
R118 T280 T281 nmod stem,cells
R119 T281 T278 pobj cells,in
R120 T282 T280 punct (,stem
R121 T283 T280 appos ES,stem
R122 T284 T281 punct ),cells
R123 T285 T281 cc and,cells
R124 T286 T281 conj mice,cells
R125 T287 T288 punct [,2
R126 T288 T262 parataxis 2,demonstrated
R127 T289 T288 punct ],2
R128 T290 T262 punct .,demonstrated
R129 T294 T295 prep In,used
R130 T296 T297 det this,study
R131 T297 T294 pobj study,In
R132 T298 T295 nsubj we,used
R133 T299 T295 aux have,used
R134 T300 T301 amod embryonic,stem
R135 T301 T302 nmod stem,cell
R136 T302 T306 npadvmod cell,mediated
R137 T303 T301 punct (,stem
R138 T304 T301 appos ES,stem
R139 T305 T302 punct ),cell
R140 T306 T308 amod mediated,transgenesis
R141 T307 T306 punct -,mediated
R142 T308 T295 dobj transgenesis,used
R143 T309 T310 aux to,test
R144 T310 T295 advcl test,used
R145 T311 T312 det the,protein
R146 T312 T310 dobj protein,test
R147 T313 T312 amod enhanced,protein
R148 T314 T312 nmod cyan,protein
R149 T315 T312 amod fluorescent,protein
R150 T316 T312 punct (,protein
R151 T317 T312 appos ECFP,protein
R152 T318 T312 punct ),protein
R153 T319 T312 cc and,protein
R154 T320 T321 amod enhanced,protein
R155 T321 T312 conj protein,protein
R156 T322 T321 amod yellow,protein
R157 T323 T321 amod fluorescent,protein
R158 T324 T321 punct (,protein
R159 T325 T321 appos EYFP,protein
R160 T326 T312 punct ),protein
R161 T327 T312 punct ", ",protein
R162 T328 T329 nummod two,variants
R163 T329 T312 appos variants,protein
R164 T330 T329 amod mutant,variants
R165 T331 T330 cc and,mutant
R166 T332 T333 advmod spectrally,distinct
R167 T333 T330 conj distinct,mutant
R168 T334 T329 compound color,variants
R169 T335 T329 prep of,variants
R170 T336 T337 amod wild,type
R171 T337 T338 nmod type,GFP
R172 T338 T335 pobj GFP,of
R173 T339 T337 punct (,type
R174 T340 T337 appos wt,type
R175 T341 T338 punct ),GFP
R176 T342 T295 punct .,used
R177 T344 T345 nsubj We,tested
R178 T346 T345 aux have,tested
R179 T347 T345 advmod also,tested
R180 T348 T345 dobj DsRed1,tested
R181 T349 T348 punct ", ",DsRed1
R182 T350 T351 det the,reporter
R183 T351 T348 appos reporter,DsRed1
R184 T352 T351 amod novel,reporter
R185 T353 T354 amod red,protein
R186 T354 T351 compound protein,reporter
R187 T355 T354 amod fluorescent,protein
R188 T356 T357 advmod recently,cloned
R189 T357 T351 acl cloned,reporter
R190 T358 T357 prep from,cloned
R191 T359 T360 det the,coral
R192 T360 T358 pobj coral,from
R193 T361 T360 compound Discostoma,coral
R194 T362 T357 prep by,cloned
R195 T363 T362 pobj virtue,by
R196 T364 T363 prep of,virtue
R197 T365 T366 poss its,homology
R198 T366 T364 pobj homology,of
R199 T367 T366 prep to,homology
R200 T368 T367 pobj GFP,to
R201 T369 T345 punct .,tested
R202 T371 T372 prep To,established
R203 T373 T374 det this,end
R204 T374 T371 pobj end,To
R205 T375 T372 punct ", ",established
R206 T376 T372 nsubj we,established
R207 T377 T372 aux have,established
R208 T378 T372 dobj lines,established
R209 T379 T378 prep of,lines
R210 T380 T381 compound ES,cells
R211 T381 T379 pobj cells,of
R212 T382 T383 advmod together,with
R213 T383 T372 prep with,established
R214 T384 T385 amod viable,mice
R215 T385 T383 pobj mice,with
R216 T386 T384 cc and,viable
R217 T387 T384 conj fertile,viable
R218 T388 T385 acl having,mice
R219 T389 T390 amod widespread,expression
R220 T390 T388 dobj expression,having
R221 T391 T390 prep of,expression
R222 T392 T393 preconj either,reporters
R223 T393 T391 pobj reporters,of
R224 T394 T393 det the,reporters
R225 T395 T393 nmod ECFP,reporters
R226 T396 T395 cc or,ECFP
R227 T397 T395 conj EYFP,ECFP
R228 T398 T399 compound GFP,variant
R229 T399 T393 compound variant,reporters
R230 T400 T399 punct -,variant
R231 T401 T372 punct .,established
R232 T403 T404 advmod However,were
R233 T405 T404 punct ", ",were
R234 T406 T404 nsubj we,were
R235 T407 T404 acomp unable,were
R236 T408 T409 aux to,generate
R237 T409 T407 xcomp generate,unable
R238 T410 T411 amod equivalent,lines
R239 T411 T409 dobj lines,generate
R240 T412 T411 compound DsRed1,lines
R241 T413 T404 punct ", ",were
R242 T414 T404 advcl suggesting,were
R243 T415 T416 mark that,is
R244 T416 T414 advcl is,suggesting
R245 T417 T416 nsubj DsRed1,is
R246 T418 T416 neg not,is
R247 T419 T420 advmod developmentally,neutral
R248 T420 T416 acomp neutral,is
R249 T421 T416 cc or,is
R25 T180 T181 amod Non-invasive,reporters
R250 T422 T423 mark that,sustained
R251 T423 T416 conj sustained,is
R252 T424 T425 compound transgene,expression
R253 T425 T423 nsubjpass expression,sustained
R254 T426 T423 aux can,sustained
R255 T427 T423 neg not,sustained
R256 T428 T423 auxpass be,sustained
R257 T429 T423 advmod constitutively,sustained
R258 T430 T404 punct .,were
R259 T432 T433 amod Balanced,chimeras
R26 T181 T183 nsubj reporters,revolutionized
R260 T433 T450 nsubj chimeras,demonstrate
R261 T434 T435 punct (,diploid
R262 T435 T432 parataxis diploid,Balanced
R263 T436 T435 nsubj diploid,diploid
R264 T437 T435 punct <,diploid
R265 T438 T435 punct -,diploid
R266 T439 T435 punct >,diploid
R267 T440 T435 punct ),diploid
R268 T441 T432 cc and,Balanced
R269 T442 T432 conj polarized,Balanced
R27 T182 T181 amod autofluorescent,reporters
R270 T443 T444 punct (,diploid
R271 T444 T442 parataxis diploid,polarized
R272 T445 T444 nsubj tetraploid,diploid
R273 T446 T444 punct <,diploid
R274 T447 T444 punct -,diploid
R275 T448 T444 punct >,diploid
R276 T449 T444 punct ),diploid
R277 T451 T433 acl comprising,chimeras
R278 T452 T451 dobj combinations,comprising
R279 T453 T452 prep of,combinations
R28 T184 T183 aux have,revolutionized
R280 T454 T455 det the,ECFP
R281 T455 T453 pobj ECFP,of
R282 T456 T455 cc and,ECFP
R283 T457 T455 conj EYFP,ECFP
R284 T458 T459 compound ES,cells
R285 T459 T455 appos cells,ECFP
R286 T460 T459 cc and,cells
R287 T461 T460 punct /,and
R288 T462 T460 cc or,and
R289 T463 T459 conj embryos,cells
R29 T185 T186 compound lineage,labeling
R290 T464 T450 punct ", ",demonstrate
R291 T465 T466 mark that,distinguished
R292 T466 T450 ccomp distinguished,demonstrate
R293 T467 T466 nsubjpass populations,distinguished
R294 T468 T467 prep of,populations
R295 T469 T468 pobj cells,of
R296 T470 T467 acl expressing,populations
R297 T471 T472 det each,reporter
R298 T472 T470 dobj reporter,expressing
R299 T473 T472 amod individual,reporter
R30 T186 T183 dobj labeling,revolutionized
R300 T474 T466 aux can,distinguished
R301 T475 T466 auxpass be,distinguished
R302 T476 T466 prep within,distinguished
R303 T477 T478 det a,animal
R304 T478 T476 pobj animal,within
R305 T479 T478 amod single,animal
R306 T480 T450 punct .,demonstrate
R307 T484 T485 nmod GFP,reporters
R308 T485 T487 nsubj reporters,are
R309 T486 T484 amod variant,GFP
R31 T187 T183 prep in,revolutionized
R310 T488 T487 acomp unique,are
R311 T489 T488 prep in,unique
R312 T490 T489 pcomp allowing,in
R313 T491 T492 amod non-invasive,visualization
R314 T492 T490 dobj visualization,allowing
R315 T493 T492 amod multi-spectral,visualization
R316 T494 T490 prep in,allowing
R317 T495 T496 amod live,samples
R318 T496 T494 pobj samples,in
R319 T497 T487 punct .,are
R32 T188 T189 det an,array
R320 T499 T500 det The,cells
R321 T500 T508 nsubj cells,provide
R322 T501 T502 npadvmod ECFP,expressing
R323 T502 T500 amod expressing,cells
R324 T503 T501 cc and,ECFP
R325 T504 T501 conj EYFP,ECFP
R326 T505 T502 punct -,expressing
R327 T506 T500 amod transgenic,cells
R328 T507 T500 compound ES,cells
R329 T509 T500 cc and,cells
R33 T189 T187 pobj array,in
R330 T510 T500 conj mice,cells
R331 T511 T512 dep that,generated
R332 T512 T500 relcl generated,cells
R333 T513 T512 nsubj we,generated
R334 T514 T512 aux have,generated
R335 T515 T508 dobj sources,provide
R336 T516 T515 prep of,sources
R337 T517 T516 pobj cells,of
R338 T518 T517 cc and,cells
R339 T519 T517 conj tissues,cells
R34 T190 T189 prep of,array
R340 T520 T508 prep for,provide
R341 T521 T522 amod combinatorial,experiments
R342 T522 T520 pobj experiments,for
R343 T523 T522 punct ", ",experiments
R344 T524 T525 amod double,tagged
R345 T525 T522 amod tagged,experiments
R346 T526 T525 punct -,tagged
R347 T527 T522 compound recombination,experiments
R348 T528 T522 punct ", ",experiments
R349 T529 T522 conj chimeras,experiments
R35 T191 T192 amod different,organisms
R350 T530 T529 cc or,chimeras
R351 T531 T529 conj transplantations,chimeras
R352 T532 T508 punct .,provide
R36 T192 T190 pobj organisms,of
R37 T193 T183 punct .,revolutionized
R38 T195 T196 prep In,gained
R39 T197 T198 amod recent,years
R40 T198 T195 pobj years,In
R41 T199 T200 amod green,protein
R42 T200 T196 nsubj protein,gained
R43 T201 T200 amod fluorescent,protein
R44 T202 T200 punct (,protein
R45 T203 T200 appos GFP,protein
R46 T204 T200 punct ),protein
R47 T205 T200 prep from,protein
R48 T206 T207 det the,jellyfish
R49 T207 T205 pobj jellyfish,from
R50 T208 T207 amod bioluminescent,jellyfish
R51 T209 T210 compound Aequoria,Victoria
R52 T210 T207 appos Victoria,jellyfish
R53 T211 T196 aux has,gained
R54 T212 T196 dobj popularity,gained
R55 T213 T196 prep in,gained
R56 T214 T215 npadvmod mouse,transgenic
R57 T215 T216 amod transgenic,regimes
R58 T216 T213 pobj regimes,in
R59 T217 T215 cc and,transgenic
R60 T218 T219 npadvmod gene,targeting
R61 T219 T215 conj targeting,transgenic
R62 T220 T221 punct [,1
R63 T221 T196 parataxis 1,gained
R64 T222 T221 punct ],1
R65 T223 T196 punct .,gained
R66 T225 T226 nsubj It,offers
R67 T227 T228 amod several,advantages
R68 T228 T226 dobj advantages,offers
R69 T229 T228 prep over,advantages
R70 T230 T231 amod conventional,reporters
R71 T231 T229 pobj reporters,over
R72 T232 T233 npadvmod gene,based
R73 T233 T231 amod based,reporters
R74 T234 T233 punct -,based
R75 T235 T228 punct ", ",advantages
R76 T236 T237 amod such,as
R77 T237 T228 prep as,advantages
R78 T238 T237 pobj lacZ,as
R79 T239 T238 cc and,lacZ
R80 T240 T241 compound alkaline,phosphatase
R81 T241 T238 conj phosphatase,lacZ
R82 T242 T226 punct ", ",offers
R83 T243 T244 mark in,require
R84 T244 T226 advcl require,offers
R85 T245 T244 mark that,require
R86 T246 T247 poss its,visualization
R87 T247 T244 nsubj visualization,require
R88 T248 T244 aux does,require
R89 T249 T244 neg not,require
R90 T250 T251 det a,substrate
R91 T251 T244 dobj substrate,require
R92 T252 T251 amod chromogenic,substrate
R93 T253 T244 cc and,require
R94 T254 T255 aux can,realized
R95 T255 T244 conj realized,require
R96 T256 T255 auxpass be,realized
R97 T257 T258 advmod in,vivo
R98 T258 T255 advmod vivo,realized
R99 T259 T226 punct .,offers