PMC:116589 JSON TXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T157	0-9	JJ	denotes	Embryonic
T159	0-127	sentence	denotes	Embryonic stem cells and mice expressing different GFP variants for multiple non-invasive reporter usage within a single animal
T160	10-14	NN	denotes	stem
T158	15-20	NNS	denotes	cells
T161	21-24	CC	denotes	and
T162	25-29	NNS	denotes	mice
T163	30-40	VBG	denotes	expressing
T164	41-50	JJ	denotes	different
T166	51-54	NN	denotes	GFP
T165	55-63	NNS	denotes	variants
T167	64-67	IN	denotes	for
T168	68-76	JJ	denotes	multiple
T170	77-89	JJ	denotes	non-invasive
T171	90-98	NN	denotes	reporter
T169	99-104	NN	denotes	usage
T172	105-111	IN	denotes	within
T173	112-113	DT	denotes	a
T175	114-120	JJ	denotes	single
T174	121-127	NN	denotes	animal
T176	127-128	sentence	denotes
T177	138-148	sentence	denotes	Background
T178	138-148	NN	denotes	Background
T179	148-260	sentence	denotes	Non-invasive autofluorescent reporters have revolutionized lineage labeling in an array of different organisms.
T180	149-161	JJ	denotes	Non-invasive
T182	162-177	JJ	denotes	autofluorescent
T181	178-187	NNS	denotes	reporters
T184	188-192	VBP	denotes	have
T183	193-207	VBN	denotes	revolutionized
T185	208-215	NN	denotes	lineage
T186	216-224	NN	denotes	labeling
T187	225-227	IN	denotes	in
T188	228-230	DT	denotes	an
T189	231-236	NN	denotes	array
T190	237-239	IN	denotes	of
T191	240-249	JJ	denotes	different
T192	250-259	NNS	denotes	organisms
T193	259-260	.	denotes	.
T194	260-434	sentence	denotes	In recent years green fluorescent protein (GFP) from the bioluminescent jellyfish Aequoria Victoria has gained popularity in mouse transgenic and gene targeting regimes [1].
T195	261-263	IN	denotes	In
T197	264-270	JJ	denotes	recent
T198	271-276	NNS	denotes	years
T199	277-282	JJ	denotes	green
T201	283-294	JJ	denotes	fluorescent
T200	295-302	NN	denotes	protein
T202	303-304	-LRB-	denotes	(
T203	304-307	NN	denotes	GFP
T204	307-308	-RRB-	denotes	)
T205	309-313	IN	denotes	from
T206	314-317	DT	denotes	the
T208	318-332	JJ	denotes	bioluminescent
T207	333-342	NN	denotes	jellyfish
T209	343-351	NNP	denotes	Aequoria
T210	352-360	NNP	denotes	Victoria
T211	361-364	VBZ	denotes	has
T196	365-371	VBN	denotes	gained
T212	372-382	NN	denotes	popularity
T213	383-385	IN	denotes	in
T214	386-391	NN	denotes	mouse
T215	392-402	JJ	denotes	transgenic
T217	403-406	CC	denotes	and
T218	407-411	NN	denotes	gene
T219	412-421	VBG	denotes	targeting
T216	422-429	NNS	denotes	regimes
T220	430-431	-LRB-	denotes	[
T221	431-432	CD	denotes	1
T222	432-433	-RRB-	denotes	]
T223	433-434	.	denotes	.
T224	434-638	sentence	denotes	It offers several advantages over conventional gene-based reporters, such as lacZ and alkaline phosphatase, in that its visualization does not require a chromogenic substrate and can be realized in vivo.
T225	435-437	PRP	denotes	It
T226	438-444	VBZ	denotes	offers
T227	445-452	JJ	denotes	several
T228	453-463	NNS	denotes	advantages
T229	464-468	IN	denotes	over
T230	469-481	JJ	denotes	conventional
T232	482-486	NN	denotes	gene
T234	486-487	HYPH	denotes	-
T233	487-492	VBN	denotes	based
T231	493-502	NNS	denotes	reporters
T235	502-504	,	denotes	,
T236	504-508	JJ	denotes	such
T237	509-511	IN	denotes	as
T238	512-516	NN	denotes	lacZ
T239	517-520	CC	denotes	and
T240	521-529	NN	denotes	alkaline
T241	530-541	NN	denotes	phosphatase
T242	541-543	,	denotes	,
T243	543-545	IN	denotes	in
T245	546-550	IN	denotes	that
T246	551-554	PRP$	denotes	its
T247	555-568	NN	denotes	visualization
T248	569-573	VBZ	denotes	does
T249	574-577	RB	denotes	not
T244	578-585	VB	denotes	require
T250	586-587	DT	denotes	a
T252	588-599	JJ	denotes	chromogenic
T251	600-609	NN	denotes	substrate
T253	610-613	CC	denotes	and
T254	614-617	MD	denotes	can
T256	618-620	VB	denotes	be
T255	621-629	VBN	denotes	realized
T257	630-632	FW	denotes	in
T258	633-637	FW	denotes	vivo
T259	637-638	.	denotes	.
T260	638-799	sentence	denotes	We have previously demonstrated the utility and developmental neutrality of enhanced green fluorescent protein (EGFP) in embryonic stem (ES) cells and mice [2].
T261	639-641	PRP	denotes	We
T263	642-646	VBP	denotes	have
T264	647-657	RB	denotes	previously
T262	658-670	VBN	denotes	demonstrated
T265	671-674	DT	denotes	the
T266	675-682	NN	denotes	utility
T267	683-686	CC	denotes	and
T268	687-700	JJ	denotes	developmental
T269	701-711	NN	denotes	neutrality
T270	712-714	IN	denotes	of
T271	715-723	VBN	denotes	enhanced
T273	724-729	JJ	denotes	green
T274	730-741	JJ	denotes	fluorescent
T272	742-749	NN	denotes	protein
T275	750-751	-LRB-	denotes	(
T276	751-755	NN	denotes	EGFP
T277	755-756	-RRB-	denotes	)
T278	757-759	IN	denotes	in
T279	760-769	JJ	denotes	embryonic
T280	770-774	NN	denotes	stem
T282	775-776	-LRB-	denotes	(
T283	776-778	NN	denotes	ES
T284	778-779	-RRB-	denotes	)
T281	780-785	NNS	denotes	cells
T285	786-789	CC	denotes	and
T286	790-794	NNS	denotes	mice
T287	795-796	-LRB-	denotes	[
T288	796-797	CD	denotes	2
T289	797-798	-RRB-	denotes	]
T290	798-799	.	denotes	.
T291	799-808	sentence	denotes	Results
T292	801-808	NNS	denotes	Results
T293	808-1056	sentence	denotes	In this study we have used embryonic stem (ES) cell-mediated transgenesis to test the enhanced cyan fluorescent protein (ECFP) and enhanced yellow fluorescent protein (EYFP), two mutant and spectrally distinct color variants of wild type (wt) GFP.
T294	809-811	IN	denotes	In
T296	812-816	DT	denotes	this
T297	817-822	NN	denotes	study
T298	823-825	PRP	denotes	we
T299	826-830	VBP	denotes	have
T295	831-835	VBN	denotes	used
T300	836-845	JJ	denotes	embryonic
T301	846-850	NN	denotes	stem
T303	851-852	-LRB-	denotes	(
T304	852-854	NN	denotes	ES
T305	854-855	-RRB-	denotes	)
T302	856-860	NN	denotes	cell
T307	860-861	HYPH	denotes	-
T306	861-869	VBN	denotes	mediated
T308	870-882	NN	denotes	transgenesis
T309	883-885	TO	denotes	to
T310	886-890	VB	denotes	test
T311	891-894	DT	denotes	the
T313	895-903	VBN	denotes	enhanced
T314	904-908	NN	denotes	cyan
T315	909-920	JJ	denotes	fluorescent
T312	921-928	NN	denotes	protein
T316	929-930	-LRB-	denotes	(
T317	930-934	NN	denotes	ECFP
T318	934-935	-RRB-	denotes	)
T319	936-939	CC	denotes	and
T320	940-948	VBN	denotes	enhanced
T322	949-955	JJ	denotes	yellow
T323	956-967	JJ	denotes	fluorescent
T321	968-975	NN	denotes	protein
T324	976-977	-LRB-	denotes	(
T325	977-981	NN	denotes	EYFP
T326	981-982	-RRB-	denotes	)
T327	982-984	,	denotes	,
T328	984-987	CD	denotes	two
T330	988-994	JJ	denotes	mutant
T331	995-998	CC	denotes	and
T332	999-1009	RB	denotes	spectrally
T333	1010-1018	JJ	denotes	distinct
T334	1019-1024	NN	denotes	color
T329	1025-1033	NNS	denotes	variants
T335	1034-1036	IN	denotes	of
T336	1037-1041	JJ	denotes	wild
T337	1042-1046	NN	denotes	type
T339	1047-1048	-LRB-	denotes	(
T340	1048-1050	NN	denotes	wt
T341	1050-1051	-RRB-	denotes	)
T338	1052-1055	NN	denotes	GFP
T342	1055-1056	.	denotes	.
T343	1056-1203	sentence	denotes	We have also tested DsRed1, the novel red fluorescent protein reporter recently cloned from the Discostoma coral by virtue of its homology to GFP.
T344	1057-1059	PRP	denotes	We
T346	1060-1064	VBP	denotes	have
T347	1065-1069	RB	denotes	also
T345	1070-1076	VBN	denotes	tested
T348	1077-1083	NN	denotes	DsRed1
T349	1083-1085	,	denotes	,
T350	1085-1088	DT	denotes	the
T352	1089-1094	JJ	denotes	novel
T353	1095-1098	JJ	denotes	red
T355	1099-1110	JJ	denotes	fluorescent
T354	1111-1118	NN	denotes	protein
T351	1119-1127	NN	denotes	reporter
T356	1128-1136	RB	denotes	recently
T357	1137-1143	VBN	denotes	cloned
T358	1144-1148	IN	denotes	from
T359	1149-1152	DT	denotes	the
T361	1153-1163	NN	denotes	Discostoma
T360	1164-1169	NN	denotes	coral
T362	1170-1172	IN	denotes	by
T363	1173-1179	NN	denotes	virtue
T364	1180-1182	IN	denotes	of
T365	1183-1186	PRP$	denotes	its
T366	1187-1195	NN	denotes	homology
T367	1196-1198	IN	denotes	to
T368	1199-1202	NN	denotes	GFP
T369	1202-1203	.	denotes	.
T370	1203-1371	sentence	denotes	To this end, we have established lines of ES cells together with viable and fertile mice having widespread expression of either the ECFP or EYFP GFP-variant reporters.
T371	1204-1206	IN	denotes	To
T373	1207-1211	DT	denotes	this
T374	1212-1215	NN	denotes	end
T375	1215-1217	,	denotes	,
T376	1217-1219	PRP	denotes	we
T377	1220-1224	VBP	denotes	have
T372	1225-1236	VBN	denotes	established
T378	1237-1242	NNS	denotes	lines
T379	1243-1245	IN	denotes	of
T380	1246-1248	NN	denotes	ES
T381	1249-1254	NNS	denotes	cells
T382	1255-1263	RB	denotes	together
T383	1264-1268	IN	denotes	with
T384	1269-1275	JJ	denotes	viable
T386	1276-1279	CC	denotes	and
T387	1280-1287	JJ	denotes	fertile
T385	1288-1292	NNS	denotes	mice
T388	1293-1299	VBG	denotes	having
T389	1300-1310	JJ	denotes	widespread
T390	1311-1321	NN	denotes	expression
T391	1322-1324	IN	denotes	of
T392	1325-1331	CC	denotes	either
T394	1332-1335	DT	denotes	the
T395	1336-1340	NN	denotes	ECFP
T396	1341-1343	CC	denotes	or
T397	1344-1348	NN	denotes	EYFP
T398	1349-1352	NN	denotes	GFP
T400	1352-1353	HYPH	denotes	-
T399	1353-1360	NN	denotes	variant
T393	1361-1370	NNS	denotes	reporters
T401	1370-1371	.	denotes	.
T402	1371-1551	sentence	denotes	However, we were unable to generate equivalent DsRed1 lines, suggesting that DsRed1 is not developmentally neutral or that transgene expression cannot be sustained constitutively.
T403	1372-1379	RB	denotes	However
T405	1379-1381	,	denotes	,
T406	1381-1383	PRP	denotes	we
T404	1384-1388	VBD	denotes	were
T407	1389-1395	JJ	denotes	unable
T408	1396-1398	TO	denotes	to
T409	1399-1407	VB	denotes	generate
T410	1408-1418	JJ	denotes	equivalent
T412	1419-1425	NN	denotes	DsRed1
T411	1426-1431	NNS	denotes	lines
T413	1431-1433	,	denotes	,
T414	1433-1443	VBG	denotes	suggesting
T415	1444-1448	IN	denotes	that
T417	1449-1455	NN	denotes	DsRed1
T416	1456-1458	VBZ	denotes	is
T418	1459-1462	RB	denotes	not
T419	1463-1478	RB	denotes	developmentally
T420	1479-1486	JJ	denotes	neutral
T421	1487-1489	CC	denotes	or
T422	1490-1494	IN	denotes	that
T424	1495-1504	NN	denotes	transgene
T425	1505-1515	NN	denotes	expression
T426	1516-1519	MD	denotes	can
T427	1519-1522	RB	denotes	not
T428	1523-1525	VB	denotes	be
T423	1526-1535	VBN	denotes	sustained
T429	1536-1550	RB	denotes	constitutively
T430	1550-1551	.	denotes	.
T431	1551-1819	sentence	denotes	Balanced (diploid <-> diploid) and polarized (tetraploid <-> diploid) chimeras comprising combinations of the ECFP and EYFP ES cells and/or embryos, demonstrate that populations of cells expressing each individual reporter can be distinguished within a single animal.
T432	1552-1560	JJ	denotes	Balanced
T434	1561-1562	-LRB-	denotes	(
T436	1562-1569	JJ	denotes	diploid
T437	1570-1571	SYM	denotes	<
T438	1571-1572	HYPH	denotes	-
T439	1572-1573	SYM	denotes	>
T435	1574-1581	JJ	denotes	diploid
T440	1581-1582	-RRB-	denotes	)
T441	1583-1586	CC	denotes	and
T442	1587-1596	JJ	denotes	polarized
T443	1597-1598	-LRB-	denotes	(
T445	1598-1608	JJ	denotes	tetraploid
T446	1609-1610	SYM	denotes	<
T447	1610-1611	HYPH	denotes	-
T448	1611-1612	SYM	denotes	>
T444	1613-1620	JJ	denotes	diploid
T449	1620-1621	-RRB-	denotes	)
T433	1622-1630	NNS	denotes	chimeras
T451	1631-1641	VBG	denotes	comprising
T452	1642-1654	NNS	denotes	combinations
T453	1655-1657	IN	denotes	of
T454	1658-1661	DT	denotes	the
T455	1662-1666	NN	denotes	ECFP
T456	1667-1670	CC	denotes	and
T457	1671-1675	NN	denotes	EYFP
T458	1676-1678	NN	denotes	ES
T459	1679-1684	NNS	denotes	cells
T460	1685-1688	CC	denotes	and
T461	1688-1689	HYPH	denotes	/
T462	1689-1691	CC	denotes	or
T463	1692-1699	NNS	denotes	embryos
T464	1699-1701	,	denotes	,
T450	1701-1712	VBP	denotes	demonstrate
T465	1713-1717	IN	denotes	that
T467	1718-1729	NNS	denotes	populations
T468	1730-1732	IN	denotes	of
T469	1733-1738	NNS	denotes	cells
T470	1739-1749	VBG	denotes	expressing
T471	1750-1754	DT	denotes	each
T473	1755-1765	JJ	denotes	individual
T472	1766-1774	NN	denotes	reporter
T474	1775-1778	MD	denotes	can
T475	1779-1781	VB	denotes	be
T466	1782-1795	VBN	denotes	distinguished
T476	1796-1802	IN	denotes	within
T477	1803-1804	DT	denotes	a
T479	1805-1811	JJ	denotes	single
T478	1812-1818	NN	denotes	animal
T480	1818-1819	.	denotes	.
T481	1819-1832	sentence	denotes	Conclusions
T482	1821-1832	NNS	denotes	Conclusions
T483	1832-1936	sentence	denotes	GFP variant reporters are unique in allowing non-invasive multi-spectral visualization in live samples.
T484	1833-1836	NN	denotes	GFP
T486	1837-1844	JJ	denotes	variant
T485	1845-1854	NNS	denotes	reporters
T487	1855-1858	VBP	denotes	are
T488	1859-1865	JJ	denotes	unique
T489	1866-1868	IN	denotes	in
T490	1869-1877	VBG	denotes	allowing
T491	1878-1890	JJ	denotes	non-invasive
T493	1891-1905	JJ	denotes	multi-spectral
T492	1906-1919	NN	denotes	visualization
T494	1920-1922	IN	denotes	in
T495	1923-1927	JJ	denotes	live
T496	1928-1935	NNS	denotes	samples
T497	1935-1936	.	denotes	.
T498	1936-2144	sentence	denotes	The ECFP and EYFP-expressing transgenic ES cells and mice that we have generated provide sources of cells and tissues for combinatorial, double-tagged recombination experiments, chimeras or transplantations.
T499	1937-1940	DT	denotes	The
T501	1941-1945	NN	denotes	ECFP
T503	1946-1949	CC	denotes	and
T504	1950-1954	NN	denotes	EYFP
T505	1954-1955	HYPH	denotes	-
T502	1955-1965	VBG	denotes	expressing
T506	1966-1976	JJ	denotes	transgenic
T507	1977-1979	NN	denotes	ES
T500	1980-1985	NNS	denotes	cells
T509	1986-1989	CC	denotes	and
T510	1990-1994	NNS	denotes	mice
T511	1995-1999	WDT	denotes	that
T513	2000-2002	PRP	denotes	we
T514	2003-2007	VBP	denotes	have
T512	2008-2017	VBN	denotes	generated
T508	2018-2025	VBP	denotes	provide
T515	2026-2033	NNS	denotes	sources
T516	2034-2036	IN	denotes	of
T517	2037-2042	NNS	denotes	cells
T518	2043-2046	CC	denotes	and
T519	2047-2054	NNS	denotes	tissues
T520	2055-2058	IN	denotes	for
T521	2059-2072	JJ	denotes	combinatorial
T523	2072-2074	,	denotes	,
T524	2074-2080	JJ	denotes	double
T526	2080-2081	HYPH	denotes	-
T525	2081-2087	JJ	denotes	tagged
T527	2088-2101	NN	denotes	recombination
T522	2102-2113	NNS	denotes	experiments
T528	2113-2115	,	denotes	,
T529	2115-2123	NNS	denotes	chimeras
T530	2124-2126	CC	denotes	or
T531	2127-2143	NNS	denotes	transplantations
T532	2143-2144	.	denotes	.
T646	2157-2160	DT	denotes	The
T647	2161-2165	NN	denotes	ease
T649	2166-2168	IN	denotes	of
T650	2169-2181	VBG	denotes	manipulating
T651	2182-2185	PRP$	denotes	its
T652	2186-2192	NN	denotes	genome
T653	2193-2196	VBZ	denotes	has
T654	2197-2203	VBN	denotes	helped
T648	2204-2213	VB	denotes	establish
T655	2214-2217	DT	denotes	the
T656	2218-2223	NN	denotes	mouse
T657	2224-2226	IN	denotes	as
T658	2227-2230	DT	denotes	the
T660	2231-2238	JJ	denotes	premier
T661	2239-2244	NN	denotes	model
T659	2245-2253	NN	denotes	organism
T662	2254-2257	IN	denotes	for
T663	2258-2267	JJ	denotes	mammalian
T665	2268-2275	JJ	denotes	genetic
T664	2276-2283	NNS	denotes	studies
T666	2283-2284	.	denotes	.
T667	2284-2433	sentence	denotes	Both directed and random mutagenesis approaches, including the technologies of transgenesis and gene targeting in ES cells, have become commonplace.
T668	2285-2289	CC	denotes	Both
T669	2290-2298	JJ	denotes	directed
T671	2299-2302	CC	denotes	and
T672	2303-2309	JJ	denotes	random
T673	2310-2321	NN	denotes	mutagenesis
T670	2322-2332	NNS	denotes	approaches
T675	2332-2334	,	denotes	,
T676	2334-2343	VBG	denotes	including
T677	2344-2347	DT	denotes	the
T678	2348-2360	NNS	denotes	technologies
T679	2361-2363	IN	denotes	of
T680	2364-2376	NN	denotes	transgenesis
T681	2377-2380	CC	denotes	and
T682	2381-2385	NN	denotes	gene
T683	2386-2395	NN	denotes	targeting
T684	2396-2398	IN	denotes	in
T685	2399-2401	NN	denotes	ES
T686	2402-2407	NNS	denotes	cells
T687	2407-2409	,	denotes	,
T688	2409-2413	VBP	denotes	have
T674	2414-2420	VBN	denotes	become
T689	2421-2432	JJ	denotes	commonplace
T690	2432-2433	.	denotes	.
T691	2433-2600	sentence	denotes	Genetically manipulated mice, often incorporating gene-based reporters, are frequently being used to model and understand mammalian development and disease processes.
T692	2434-2445	RB	denotes	Genetically
T693	2446-2457	VBN	denotes	manipulated
T694	2458-2462	NNS	denotes	mice
T696	2462-2464	,	denotes	,
T697	2464-2469	RB	denotes	often
T698	2470-2483	VBG	denotes	incorporating
T699	2484-2488	NN	denotes	gene
T701	2488-2489	HYPH	denotes	-
T700	2489-2494	VBN	denotes	based
T702	2495-2504	NNS	denotes	reporters
T703	2504-2506	,	denotes	,
T704	2506-2509	VBP	denotes	are
T705	2510-2520	RB	denotes	frequently
T706	2521-2526	VBG	denotes	being
T695	2527-2531	VBN	denotes	used
T707	2532-2534	TO	denotes	to
T708	2535-2540	VB	denotes	model
T709	2541-2544	CC	denotes	and
T710	2545-2555	VB	denotes	understand
T711	2556-2565	JJ	denotes	mammalian
T712	2566-2577	NN	denotes	development
T713	2578-2581	CC	denotes	and
T714	2582-2589	NN	denotes	disease
T715	2590-2599	NNS	denotes	processes
T716	2599-2600	.	denotes	.
T717	2600-2868	sentence	denotes	Fluorescent protein reporters currently represent a superior alternative to other gene-based reporters such as the bacterial lacZ or human placental alkaline phosphatase in that their visualization is non-invasive, and as such does not require chromogenic substrates.
T718	2601-2612	JJ	denotes	Fluorescent
T719	2613-2620	NN	denotes	protein
T720	2621-2630	NNS	denotes	reporters
T722	2631-2640	RB	denotes	currently
T721	2641-2650	VBP	denotes	represent
T723	2651-2652	DT	denotes	a
T725	2653-2661	JJ	denotes	superior
T724	2662-2673	NN	denotes	alternative
T726	2674-2676	IN	denotes	to
T727	2677-2682	JJ	denotes	other
T729	2683-2687	NN	denotes	gene
T731	2687-2688	HYPH	denotes	-
T730	2688-2693	VBN	denotes	based
T728	2694-2703	NNS	denotes	reporters
T732	2704-2708	JJ	denotes	such
T733	2709-2711	IN	denotes	as
T734	2712-2715	DT	denotes	the
T736	2716-2725	JJ	denotes	bacterial
T735	2726-2730	NN	denotes	lacZ
T737	2731-2733	CC	denotes	or
T738	2734-2739	JJ	denotes	human
T739	2740-2749	JJ	denotes	placental
T741	2750-2758	JJ	denotes	alkaline
T740	2759-2770	NN	denotes	phosphatase
T742	2771-2773	IN	denotes	in
T744	2774-2778	DT	denotes	that
T745	2779-2784	PRP$	denotes	their
T746	2785-2798	NN	denotes	visualization
T743	2799-2801	VBZ	denotes	is
T747	2802-2814	JJ	denotes	non-invasive
T748	2814-2816	,	denotes	,
T749	2816-2819	CC	denotes	and
T750	2820-2822	IN	denotes	as
T752	2823-2827	JJ	denotes	such
T753	2828-2832	VBZ	denotes	does
T754	2833-2836	RB	denotes	not
T751	2837-2844	VB	denotes	require
T755	2845-2856	JJ	denotes	chromogenic
T756	2857-2867	NNS	denotes	substrates
T757	2867-2868	.	denotes	.
T758	2868-2990	sentence	denotes	Fluorescence can be monitored in real-time in vivo and in situ, and has the added advantage in that it can be quantified.
T759	2869-2881	NN	denotes	Fluorescence
T761	2882-2885	MD	denotes	can
T762	2886-2888	VB	denotes	be
T760	2889-2898	VBN	denotes	monitored
T763	2899-2901	IN	denotes	in
T764	2902-2906	JJ	denotes	real
T766	2906-2907	HYPH	denotes	-
T765	2907-2911	NN	denotes	time
T767	2912-2914	FW	denotes	in
T768	2915-2919	FW	denotes	vivo
T769	2920-2923	CC	denotes	and
T770	2924-2926	FW	denotes	in
T771	2927-2931	FW	denotes	situ
T772	2931-2933	,	denotes	,
T773	2933-2936	CC	denotes	and
T774	2937-2940	VBZ	denotes	has
T775	2941-2944	DT	denotes	the
T777	2945-2950	VBN	denotes	added
T776	2951-2960	NN	denotes	advantage
T778	2961-2963	IN	denotes	in
T780	2964-2968	IN	denotes	that
T781	2969-2971	PRP	denotes	it
T782	2972-2975	MD	denotes	can
T783	2976-2978	VB	denotes	be
T779	2979-2989	VBN	denotes	quantified
T784	2989-2990	.	denotes	.
T785	2990-3160	sentence	denotes	The prototype fluorescent protein reporter is green fluorescent protein (GFP, reviewed in [3]), which is derived from the bioluminescent jellyfish Aequorea Victoria [1].
T786	2991-2994	DT	denotes	The
T788	2995-3004	NN	denotes	prototype
T789	3005-3016	JJ	denotes	fluorescent
T790	3017-3024	NN	denotes	protein
T787	3025-3033	NN	denotes	reporter
T791	3034-3036	VBZ	denotes	is
T792	3037-3042	JJ	denotes	green
T794	3043-3054	JJ	denotes	fluorescent
T793	3055-3062	NN	denotes	protein
T795	3063-3064	-LRB-	denotes	(
T796	3064-3067	NN	denotes	GFP
T797	3067-3069	,	denotes	,
T798	3069-3077	VBN	denotes	reviewed
T799	3078-3080	IN	denotes	in
T800	3081-3082	-LRB-	denotes	[
T801	3082-3083	CD	denotes	3
T802	3083-3084	-RRB-	denotes	]
T803	3084-3085	-RRB-	denotes	)
T804	3085-3087	,	denotes	,
T805	3087-3092	WDT	denotes	which
T807	3093-3095	VBZ	denotes	is
T806	3096-3103	VBN	denotes	derived
T808	3104-3108	IN	denotes	from
T809	3109-3112	DT	denotes	the
T811	3113-3127	JJ	denotes	bioluminescent
T810	3128-3137	NN	denotes	jellyfish
T812	3138-3146	NNP	denotes	Aequorea
T813	3147-3155	NNP	denotes	Victoria
T814	3156-3157	-LRB-	denotes	[
T815	3157-3158	CD	denotes	1
T816	3158-3159	-RRB-	denotes	]
T817	3159-3160	.	denotes	.
T818	3160-3382	sentence	denotes	Green fluorescent variants of wild type GFP (wtGFP) with improved thermostability and fluorescence emission, including enhanced green fluorescent protein (EGFP, [4]), and mMGFP [5], have gained popularity for use in mice.
T819	3161-3166	JJ	denotes	Green
T821	3167-3178	JJ	denotes	fluorescent
T820	3179-3187	NNS	denotes	variants
T823	3188-3190	IN	denotes	of
T824	3191-3195	JJ	denotes	wild
T825	3196-3200	NN	denotes	type
T826	3201-3204	NN	denotes	GFP
T827	3205-3206	-LRB-	denotes	(
T828	3206-3211	NN	denotes	wtGFP
T829	3211-3212	-RRB-	denotes	)
T830	3213-3217	IN	denotes	with
T831	3218-3226	VBN	denotes	improved
T832	3227-3242	NN	denotes	thermostability
T833	3243-3246	CC	denotes	and
T834	3247-3259	NN	denotes	fluorescence
T835	3260-3268	NN	denotes	emission
T836	3268-3270	,	denotes	,
T837	3270-3279	VBG	denotes	including
T838	3280-3288	VBN	denotes	enhanced
T840	3289-3294	JJ	denotes	green
T841	3295-3306	JJ	denotes	fluorescent
T839	3307-3314	NN	denotes	protein
T842	3315-3316	-LRB-	denotes	(
T843	3316-3320	NN	denotes	EGFP
T844	3320-3322	,	denotes	,
T845	3322-3323	-LRB-	denotes	[
T846	3323-3324	CD	denotes	4
T847	3324-3325	-RRB-	denotes	]
T848	3325-3326	-RRB-	denotes	)
T849	3326-3328	,	denotes	,
T850	3328-3331	CC	denotes	and
T851	3332-3337	NN	denotes	mMGFP
T852	3338-3339	-LRB-	denotes	[
T853	3339-3340	CD	denotes	5
T854	3340-3341	-RRB-	denotes	]
T855	3341-3343	,	denotes	,
T856	3343-3347	VBP	denotes	have
T822	3348-3354	VBN	denotes	gained
T857	3355-3365	NN	denotes	popularity
T858	3366-3369	IN	denotes	for
T859	3370-3373	NN	denotes	use
T860	3374-3376	IN	denotes	in
T861	3377-3381	NNS	denotes	mice
T862	3381-3382	.	denotes	.
T863	3382-3618	sentence	denotes	Recently however, several additional mutants of wtGFP, with altered excitation and emission spectral profiles (i.e. fluoresce in colors other than green), as well as improved thermostability and fluorescence, have been described [4,6].
T864	3383-3391	RB	denotes	Recently
T866	3392-3399	RB	denotes	however
T867	3399-3401	,	denotes	,
T868	3401-3408	JJ	denotes	several
T870	3409-3419	JJ	denotes	additional
T869	3420-3427	NNS	denotes	mutants
T871	3428-3430	IN	denotes	of
T872	3431-3436	NN	denotes	wtGFP
T873	3436-3438	,	denotes	,
T874	3438-3442	IN	denotes	with
T875	3443-3450	VBN	denotes	altered
T877	3451-3461	NN	denotes	excitation
T878	3462-3465	CC	denotes	and
T879	3466-3474	NN	denotes	emission
T880	3475-3483	JJ	denotes	spectral
T876	3484-3492	NNS	denotes	profiles
T881	3493-3494	-LRB-	denotes	(
T883	3494-3498	FW	denotes	i.e.
T882	3499-3508	VB	denotes	fluoresce
T884	3509-3511	IN	denotes	in
T885	3512-3518	NNS	denotes	colors
T886	3519-3524	JJ	denotes	other
T887	3525-3529	IN	denotes	than
T888	3530-3535	JJ	denotes	green
T889	3535-3536	-RRB-	denotes	)
T890	3536-3538	,	denotes	,
T891	3538-3540	RB	denotes	as
T893	3541-3545	RB	denotes	well
T892	3546-3548	IN	denotes	as
T894	3549-3557	JJ	denotes	improved
T895	3558-3573	NN	denotes	thermostability
T896	3574-3577	CC	denotes	and
T897	3578-3590	NN	denotes	fluorescence
T898	3590-3592	,	denotes	,
T899	3592-3596	VBP	denotes	have
T900	3597-3601	VBN	denotes	been
T865	3602-3611	VBN	denotes	described
T901	3612-3613	-LRB-	denotes	[
T903	3613-3614	CD	denotes	4
T904	3614-3615	,	denotes	,
T902	3615-3616	CD	denotes	6
T905	3616-3617	-RRB-	denotes	]
T906	3617-3618	.	denotes	.
T907	3618-3758	sentence	denotes	Dual color imaging of several GFP variant proteins is a very attractive prospect and has become a standard procedure in cell biology [7,8].
T908	3619-3623	JJ	denotes	Dual
T909	3624-3629	NN	denotes	color
T910	3630-3637	NN	denotes	imaging
T912	3638-3640	IN	denotes	of
T913	3641-3648	JJ	denotes	several
T915	3649-3652	NN	denotes	GFP
T916	3653-3660	NN	denotes	variant
T914	3661-3669	NN	denotes	proteins
T911	3670-3672	VBZ	denotes	is
T917	3673-3674	DT	denotes	a
T919	3675-3679	RB	denotes	very
T920	3680-3690	JJ	denotes	attractive
T918	3691-3699	NN	denotes	prospect
T921	3700-3703	CC	denotes	and
T922	3704-3707	VBZ	denotes	has
T923	3708-3714	VBN	denotes	become
T924	3715-3716	DT	denotes	a
T926	3717-3725	JJ	denotes	standard
T925	3726-3735	NN	denotes	procedure
T927	3736-3738	IN	denotes	in
T928	3739-3743	NN	denotes	cell
T929	3744-3751	NN	denotes	biology
T930	3752-3753	-LRB-	denotes	[
T932	3753-3754	CD	denotes	7
T933	3754-3755	,	denotes	,
T931	3755-3756	CD	denotes	8
T934	3756-3757	-RRB-	denotes	]
T935	3757-3758	.	denotes	.
T936	3758-3933	sentence	denotes	It has also recently been demonstrated in a subset of cells within the central nervous system (CNS) of mice using both transgenic and viral infection-based approaches [9,10].
T937	3759-3761	PRP	denotes	It
T939	3762-3765	VBZ	denotes	has
T940	3766-3770	RB	denotes	also
T941	3771-3779	RB	denotes	recently
T942	3780-3784	VBN	denotes	been
T938	3785-3797	VBN	denotes	demonstrated
T943	3798-3800	IN	denotes	in
T944	3801-3802	DT	denotes	a
T945	3803-3809	NN	denotes	subset
T946	3810-3812	IN	denotes	of
T947	3813-3818	NNS	denotes	cells
T948	3819-3825	IN	denotes	within
T949	3826-3829	DT	denotes	the
T951	3830-3837	JJ	denotes	central
T952	3838-3845	JJ	denotes	nervous
T950	3846-3852	NN	denotes	system
T953	3853-3854	-LRB-	denotes	(
T954	3854-3857	NN	denotes	CNS
T955	3857-3858	-RRB-	denotes	)
T956	3859-3861	IN	denotes	of
T957	3862-3866	NNS	denotes	mice
T958	3867-3872	VBG	denotes	using
T959	3873-3877	CC	denotes	both
T960	3878-3888	JJ	denotes	transgenic
T962	3889-3892	CC	denotes	and
T963	3893-3898	JJ	denotes	viral
T964	3899-3908	JJ	denotes	infection
T966	3908-3909	HYPH	denotes	-
T965	3909-3914	JJ	denotes	based
T961	3915-3925	NNS	denotes	approaches
T967	3926-3927	-LRB-	denotes	[
T969	3927-3928	CD	denotes	9
T970	3928-3929	,	denotes	,
T968	3929-3931	CD	denotes	10
T971	3931-3932	-RRB-	denotes	]
T972	3932-3933	.	denotes	.
T973	3933-4182	sentence	denotes	We therefore wished to investigate the feasibility of non-invasive multiple reporter imaging in mice, and as a first step we wanted to ascertain whether we could establish viable and fertile mice with widespread expression of GFP variant reporters.
T974	3934-3936	PRP	denotes	We
T976	3937-3946	RB	denotes	therefore
T975	3947-3953	VBD	denotes	wished
T977	3954-3956	TO	denotes	to
T978	3957-3968	VB	denotes	investigate
T979	3969-3972	DT	denotes	the
T980	3973-3984	NN	denotes	feasibility
T981	3985-3987	IN	denotes	of
T982	3988-4000	JJ	denotes	non-invasive
T984	4001-4009	JJ	denotes	multiple
T985	4010-4018	NN	denotes	reporter
T983	4019-4026	NN	denotes	imaging
T986	4027-4029	IN	denotes	in
T987	4030-4034	NNS	denotes	mice
T988	4034-4036	,	denotes	,
T989	4036-4039	CC	denotes	and
T990	4040-4042	IN	denotes	as
T992	4043-4044	DT	denotes	a
T994	4045-4050	JJ	denotes	first
T993	4051-4055	NN	denotes	step
T995	4056-4058	PRP	denotes	we
T991	4059-4065	VBD	denotes	wanted
T996	4066-4068	TO	denotes	to
T997	4069-4078	VB	denotes	ascertain
T998	4079-4086	IN	denotes	whether
T1000	4087-4089	PRP	denotes	we
T1001	4090-4095	MD	denotes	could
T999	4096-4105	VB	denotes	establish
T1002	4106-4112	JJ	denotes	viable
T1004	4113-4116	CC	denotes	and
T1005	4117-4124	JJ	denotes	fertile
T1003	4125-4129	NNS	denotes	mice
T1006	4130-4134	IN	denotes	with
T1007	4135-4145	JJ	denotes	widespread
T1008	4146-4156	NN	denotes	expression
T1009	4157-4159	IN	denotes	of
T1010	4160-4163	NN	denotes	GFP
T1011	4164-4171	NN	denotes	variant
T1012	4172-4181	NNS	denotes	reporters
T1013	4181-4182	.	denotes	.
T1399	4207-4209	PRP	denotes	We
T1400	4210-4216	VBD	denotes	tested
T1401	4217-4224	JJ	denotes	several
T1402	4225-4227	IN	denotes	of
T1403	4228-4231	DT	denotes	the
T1405	4232-4241	JJ	denotes	available
T1406	4242-4247	NN	denotes	wtGFP
T1404	4248-4256	NNS	denotes	variants
T1407	4257-4260	IN	denotes	for
T1408	4261-4274	JJ	denotes	developmental
T1409	4275-4285	NN	denotes	neutrality
T1410	4286-4289	CC	denotes	and
T1411	4290-4299	NN	denotes	stability
T1412	4300-4302	IN	denotes	of
T1413	4303-4313	JJ	denotes	widespread
T1414	4314-4324	NN	denotes	expression
T1415	4324-4325	.	denotes	.
T1416	4325-4510	sentence	denotes	An ES cell-mediated transgenic approach was used to evaluate the Enhanced Cyan Fluorescent Protein (ECFP) and Enhanced Yellow Fluorescent Protein (EYFP) wtGFP spectral variants [4,11].
T1417	4326-4328	DT	denotes	An
T1419	4329-4331	NN	denotes	ES
T1420	4332-4336	NN	denotes	cell
T1422	4336-4337	HYPH	denotes	-
T1421	4337-4345	VBN	denotes	mediated
T1423	4346-4356	JJ	denotes	transgenic
T1418	4357-4365	NN	denotes	approach
T1425	4366-4369	VBD	denotes	was
T1424	4370-4374	VBN	denotes	used
T1426	4375-4377	TO	denotes	to
T1427	4378-4386	VB	denotes	evaluate
T1428	4387-4390	DT	denotes	the
T1430	4391-4399	VBN	denotes	Enhanced
T1431	4400-4404	NN	denotes	Cyan
T1432	4405-4416	JJ	denotes	Fluorescent
T1429	4417-4424	NN	denotes	Protein
T1434	4425-4426	-LRB-	denotes	(
T1435	4426-4430	NN	denotes	ECFP
T1436	4430-4431	-RRB-	denotes	)
T1437	4432-4435	CC	denotes	and
T1438	4436-4444	VBD	denotes	Enhanced
T1440	4445-4451	JJ	denotes	Yellow
T1441	4452-4463	JJ	denotes	Fluorescent
T1439	4464-4471	NN	denotes	Protein
T1442	4472-4473	-LRB-	denotes	(
T1443	4473-4477	NN	denotes	EYFP
T1444	4477-4478	-RRB-	denotes	)
T1445	4479-4484	NN	denotes	wtGFP
T1446	4485-4493	JJ	denotes	spectral
T1433	4494-4502	NNS	denotes	variants
T1447	4503-4504	-LRB-	denotes	[
T1449	4504-4505	CD	denotes	4
T1450	4505-4506	,	denotes	,
T1448	4506-4508	CD	denotes	11
T1451	4508-4509	-RRB-	denotes	]
T1452	4509-4510	.	denotes	.
T1453	4510-4663	sentence	denotes	ECFP harbors six amino substitutions (Y66W, F64L, S65T, N146I, M153T, V163A) as compared to GFP, whereas EYFP harbors four (S65G, V68L, S72A and T203Y).
T1454	4511-4515	NN	denotes	ECFP
T1455	4516-4523	VBZ	denotes	harbors
T1456	4524-4527	CD	denotes	six
T1458	4528-4533	NN	denotes	amino
T1457	4534-4547	NNS	denotes	substitutions
T1459	4548-4549	-LRB-	denotes	(
T1460	4549-4553	NN	denotes	Y66W
T1462	4553-4555	,	denotes	,
T1463	4555-4559	NN	denotes	F64L
T1464	4559-4561	,	denotes	,
T1465	4561-4565	NN	denotes	S65T
T1466	4565-4567	,	denotes	,
T1467	4567-4572	NN	denotes	N146I
T1468	4572-4574	,	denotes	,
T1469	4574-4579	NN	denotes	M153T
T1470	4579-4581	,	denotes	,
T1461	4581-4586	NN	denotes	V163A
T1471	4586-4587	-RRB-	denotes	)
T1472	4588-4590	IN	denotes	as
T1473	4591-4599	VBN	denotes	compared
T1474	4600-4602	IN	denotes	to
T1475	4603-4606	NN	denotes	GFP
T1476	4606-4608	,	denotes	,
T1477	4608-4615	IN	denotes	whereas
T1479	4616-4620	NN	denotes	EYFP
T1478	4621-4628	VBZ	denotes	harbors
T1480	4629-4633	CD	denotes	four
T1481	4634-4635	-LRB-	denotes	(
T1482	4635-4639	NN	denotes	S65G
T1483	4639-4641	,	denotes	,
T1484	4641-4645	NN	denotes	V68L
T1485	4645-4647	,	denotes	,
T1486	4647-4651	NN	denotes	S72A
T1487	4652-4655	CC	denotes	and
T1488	4656-4661	NN	denotes	T203Y
T1489	4661-4662	-RRB-	denotes	)
T1490	4662-4663	.	denotes	.
T1491	4663-4818	sentence	denotes	In addition to increasing the quantum yield and solubility of the proteins these mutations shift the spectral profiles of the translation products [4,11].
T1492	4664-4666	IN	denotes	In
T1494	4667-4675	NN	denotes	addition
T1495	4676-4678	IN	denotes	to
T1496	4679-4689	VBG	denotes	increasing
T1497	4690-4693	DT	denotes	the
T1499	4694-4701	NN	denotes	quantum
T1498	4702-4707	NN	denotes	yield
T1500	4708-4711	CC	denotes	and
T1501	4712-4722	NN	denotes	solubility
T1502	4723-4725	IN	denotes	of
T1503	4726-4729	DT	denotes	the
T1504	4730-4738	NN	denotes	proteins
T1505	4739-4744	DT	denotes	these
T1506	4745-4754	NNS	denotes	mutations
T1493	4755-4760	VBP	denotes	shift
T1507	4761-4764	DT	denotes	the
T1509	4765-4773	JJ	denotes	spectral
T1508	4774-4782	NNS	denotes	profiles
T1510	4783-4785	IN	denotes	of
T1511	4786-4789	DT	denotes	the
T1513	4790-4801	NN	denotes	translation
T1512	4802-4810	NNS	denotes	products
T1514	4811-4812	-LRB-	denotes	[
T1516	4812-4813	CD	denotes	4
T1517	4813-4814	,	denotes	,
T1515	4814-4816	CD	denotes	11
T1518	4816-4817	-RRB-	denotes	]
T1519	4817-4818	.	denotes	.
T1520	4818-5003	sentence	denotes	A breakthrough in the availability of spectrally distinct autofluorescent proteins came with the cloning of six anthozoan fluorescent proteins all having 20–30% identity to wtGFP [12].
T1521	4819-4820	DT	denotes	A
T1522	4821-4833	NN	denotes	breakthrough
T1524	4834-4836	IN	denotes	in
T1525	4837-4840	DT	denotes	the
T1526	4841-4853	NN	denotes	availability
T1527	4854-4856	IN	denotes	of
T1528	4857-4867	RB	denotes	spectrally
T1529	4868-4876	JJ	denotes	distinct
T1531	4877-4892	JJ	denotes	autofluorescent
T1530	4893-4901	NN	denotes	proteins
T1523	4902-4906	VBD	denotes	came
T1532	4907-4911	IN	denotes	with
T1533	4912-4915	DT	denotes	the
T1534	4916-4923	NN	denotes	cloning
T1535	4924-4926	IN	denotes	of
T1536	4927-4930	CD	denotes	six
T1538	4931-4940	NN	denotes	anthozoan
T1539	4941-4952	JJ	denotes	fluorescent
T1537	4953-4961	NN	denotes	proteins
T1540	4962-4965	RB	denotes	all
T1541	4966-4972	VBG	denotes	having
T1542	4973-4975	CD	denotes	20
T1544	4975-4976	SYM	denotes	–
T1543	4976-4978	CD	denotes	30
T1545	4978-4979	NN	denotes	%
T1546	4980-4988	NN	denotes	identity
T1547	4989-4991	IN	denotes	to
T1548	4992-4997	NN	denotes	wtGFP
T1549	4998-4999	-LRB-	denotes	[
T1550	4999-5001	CD	denotes	12
T1551	5001-5002	-RRB-	denotes	]
T1552	5002-5003	.	denotes	.
T1553	5003-5268	sentence	denotes	Of these, DsRed1 (referred to as drFP585 by Matz et al., [12]), a 28 kDa protein isolated from the IndoPacific sea anemone Discosoma sp, posseses the longest excitation (558 nm) and emission (583 nm) wavelength maxima known for a wild-type autofluorescent protein.
T1554	5004-5006	IN	denotes	Of
T1556	5007-5012	DT	denotes	these
T1557	5012-5014	,	denotes	,
T1558	5014-5020	NN	denotes	DsRed1
T1559	5021-5022	-LRB-	denotes	(
T1560	5022-5030	VBN	denotes	referred
T1561	5031-5033	IN	denotes	to
T1562	5034-5036	IN	denotes	as
T1563	5037-5044	NN	denotes	drFP585
T1564	5045-5047	IN	denotes	by
T1565	5048-5052	NNP	denotes	Matz
T1566	5053-5055	FW	denotes	et
T1567	5056-5059	FW	denotes	al.
T1568	5059-5061	,	denotes	,
T1569	5061-5062	-LRB-	denotes	[
T1570	5062-5064	CD	denotes	12
T1571	5064-5065	-RRB-	denotes	]
T1572	5065-5066	-RRB-	denotes	)
T1573	5066-5068	,	denotes	,
T1574	5068-5069	DT	denotes	a
T1576	5070-5072	CD	denotes	28
T1577	5073-5076	NN	denotes	kDa
T1575	5077-5084	NN	denotes	protein
T1578	5085-5093	VBN	denotes	isolated
T1579	5094-5098	IN	denotes	from
T1580	5099-5102	DT	denotes	the
T1582	5103-5114	NNP	denotes	IndoPacific
T1583	5115-5118	NN	denotes	sea
T1581	5119-5126	NN	denotes	anemone
T1584	5127-5136	NNP	denotes	Discosoma
T1585	5137-5139	NNP	denotes	sp
T1586	5139-5141	,	denotes	,
T1555	5141-5149	VBZ	denotes	posseses
T1587	5150-5153	DT	denotes	the
T1589	5154-5161	JJS	denotes	longest
T1590	5162-5172	NN	denotes	excitation
T1591	5173-5174	-LRB-	denotes	(
T1593	5174-5177	CD	denotes	558
T1592	5178-5180	NN	denotes	nm
T1594	5180-5181	-RRB-	denotes	)
T1595	5182-5185	CC	denotes	and
T1596	5186-5194	NN	denotes	emission
T1597	5195-5196	-LRB-	denotes	(
T1599	5196-5199	CD	denotes	583
T1598	5200-5202	NN	denotes	nm
T1600	5202-5203	-RRB-	denotes	)
T1601	5204-5214	NN	denotes	wavelength
T1588	5215-5221	NNS	denotes	maxima
T1602	5222-5227	VBN	denotes	known
T1603	5228-5231	IN	denotes	for
T1604	5232-5233	DT	denotes	a
T1606	5234-5238	JJ	denotes	wild
T1608	5238-5239	HYPH	denotes	-
T1607	5239-5243	NN	denotes	type
T1609	5244-5259	JJ	denotes	autofluorescent
T1605	5260-5267	NN	denotes	protein
T1610	5267-5268	.	denotes	.
T1611	5268-5481	sentence	denotes	The sequence of DsRed1 has recently been modified and optimized for high levels of expression in mammalian cells by the introduction of 144 silent base pair changes corresponding to human codon usage preferences.
T1612	5269-5272	DT	denotes	The
T1613	5273-5281	NN	denotes	sequence
T1615	5282-5284	IN	denotes	of
T1616	5285-5291	NN	denotes	DsRed1
T1617	5292-5295	VBZ	denotes	has
T1618	5296-5304	RB	denotes	recently
T1619	5305-5309	VBN	denotes	been
T1614	5310-5318	VBN	denotes	modified
T1620	5319-5322	CC	denotes	and
T1621	5323-5332	VBN	denotes	optimized
T1622	5333-5336	IN	denotes	for
T1623	5337-5341	JJ	denotes	high
T1624	5342-5348	NNS	denotes	levels
T1625	5349-5351	IN	denotes	of
T1626	5352-5362	NN	denotes	expression
T1627	5363-5365	IN	denotes	in
T1628	5366-5375	JJ	denotes	mammalian
T1629	5376-5381	NNS	denotes	cells
T1630	5382-5384	IN	denotes	by
T1631	5385-5388	DT	denotes	the
T1632	5389-5401	NN	denotes	introduction
T1633	5402-5404	IN	denotes	of
T1634	5405-5408	CD	denotes	144
T1636	5409-5415	JJ	denotes	silent
T1638	5416-5420	NN	denotes	base
T1637	5421-5425	NN	denotes	pair
T1635	5426-5433	NNS	denotes	changes
T1639	5434-5447	VBG	denotes	corresponding
T1640	5448-5450	IN	denotes	to
T1641	5451-5456	JJ	denotes	human
T1642	5457-5462	NN	denotes	codon
T1643	5463-5468	NN	denotes	usage
T1644	5469-5480	NNS	denotes	preferences
T1645	5480-5481	.	denotes	.
T1646	5481-5569	sentence	denotes	We therefore also tested this novel red fluorescent protein (RFP) in ES cells and mice.
T1647	5482-5484	PRP	denotes	We
T1649	5485-5494	RB	denotes	therefore
T1650	5495-5499	RB	denotes	also
T1648	5500-5506	VBN	denotes	tested
T1651	5507-5511	DT	denotes	this
T1653	5512-5517	JJ	denotes	novel
T1654	5518-5521	JJ	denotes	red
T1655	5522-5533	JJ	denotes	fluorescent
T1652	5534-5541	NN	denotes	protein
T1656	5542-5543	-LRB-	denotes	(
T1657	5543-5546	NN	denotes	RFP
T1658	5546-5547	-RRB-	denotes	)
T1659	5548-5550	IN	denotes	in
T1660	5551-5553	NN	denotes	ES
T1661	5554-5559	NNS	denotes	cells
T1662	5560-5563	CC	denotes	and
T1663	5564-5568	NNS	denotes	mice
T1664	5568-5569	.	denotes	.
T1665	5569-5767	sentence	denotes	Plasmids were constructed for driving widespread expression of each of the gene-based fluorescent protein (FP) reporters and were tested in ES cell-mediated transgenics as described previously [2].
T1666	5570-5578	NNS	denotes	Plasmids
T1668	5579-5583	VBD	denotes	were
T1667	5584-5595	VBN	denotes	constructed
T1669	5596-5599	IN	denotes	for
T1670	5600-5607	VBG	denotes	driving
T1671	5608-5618	JJ	denotes	widespread
T1672	5619-5629	NN	denotes	expression
T1673	5630-5632	IN	denotes	of
T1674	5633-5637	DT	denotes	each
T1675	5638-5640	IN	denotes	of
T1676	5641-5644	DT	denotes	the
T1678	5645-5649	NN	denotes	gene
T1680	5649-5650	HYPH	denotes	-
T1679	5650-5655	VBN	denotes	based
T1681	5656-5667	JJ	denotes	fluorescent
T1682	5668-5675	NN	denotes	protein
T1683	5676-5677	-LRB-	denotes	(
T1684	5677-5679	NN	denotes	FP
T1685	5679-5680	-RRB-	denotes	)
T1677	5681-5690	NNS	denotes	reporters
T1686	5691-5694	CC	denotes	and
T1687	5695-5699	VBD	denotes	were
T1688	5700-5706	VBN	denotes	tested
T1689	5707-5709	IN	denotes	in
T1690	5710-5712	NN	denotes	ES
T1691	5713-5717	NN	denotes	cell
T1693	5717-5718	HYPH	denotes	-
T1692	5718-5726	JJ	denotes	mediated
T1694	5727-5738	NNS	denotes	transgenics
T1695	5739-5741	IN	denotes	as
T1696	5742-5751	VBN	denotes	described
T1697	5752-5762	RB	denotes	previously
T1698	5763-5764	-LRB-	denotes	[
T1699	5764-5765	CD	denotes	2
T1700	5765-5766	-RRB-	denotes	]
T1701	5766-5767	.	denotes	.
T1702	5767-5908	sentence	denotes	Transgenic ES cells exhibiting robust expression of ECFP or EYFP were obtained at the same frequency as we had previously obtained for EGFP.
T1703	5768-5778	JJ	denotes	Transgenic
T1705	5779-5781	NN	denotes	ES
T1704	5782-5787	NNS	denotes	cells
T1707	5788-5798	VBG	denotes	exhibiting
T1708	5799-5805	JJ	denotes	robust
T1709	5806-5816	NN	denotes	expression
T1710	5817-5819	IN	denotes	of
T1711	5820-5824	NN	denotes	ECFP
T1712	5825-5827	CC	denotes	or
T1713	5828-5832	NN	denotes	EYFP
T1714	5833-5837	VBD	denotes	were
T1706	5838-5846	VBN	denotes	obtained
T1715	5847-5849	IN	denotes	at
T1716	5850-5853	DT	denotes	the
T1718	5854-5858	JJ	denotes	same
T1717	5859-5868	NN	denotes	frequency
T1719	5869-5871	IN	denotes	as
T1721	5872-5874	PRP	denotes	we
T1722	5875-5878	VBD	denotes	had
T1723	5879-5889	RB	denotes	previously
T1720	5890-5898	VBN	denotes	obtained
T1724	5899-5902	IN	denotes	for
T1725	5903-5907	NN	denotes	EGFP
T1726	5907-5908	.	denotes	.
T1727	5908-6046	sentence	denotes	Cells expressing one or other of the two GFP variant reporters were clearly distinguishable within a mixed population (Fig. 1a,1b,1c,1d).
T1728	5909-5914	NNS	denotes	Cells
T1730	5915-5925	VBG	denotes	expressing
T1731	5926-5929	CD	denotes	one
T1733	5930-5932	CC	denotes	or
T1732	5933-5938	NN	denotes	other
T1734	5939-5941	IN	denotes	of
T1735	5942-5945	DT	denotes	the
T1737	5946-5949	CD	denotes	two
T1738	5950-5953	NN	denotes	GFP
T1739	5954-5961	JJ	denotes	variant
T1736	5962-5971	NNS	denotes	reporters
T1729	5972-5976	VBD	denotes	were
T1740	5977-5984	RB	denotes	clearly
T1741	5985-6000	JJ	denotes	distinguishable
T1742	6001-6007	IN	denotes	within
T1743	6008-6009	DT	denotes	a
T1745	6010-6015	VBN	denotes	mixed
T1744	6016-6026	NN	denotes	population
T1746	6027-6028	-LRB-	denotes	(
T1748	6028-6032	NN	denotes	Fig.
T1749	6033-6035	NN	denotes	1a
T1750	6035-6036	,	denotes	,
T1751	6036-6038	NN	denotes	1b
T1752	6038-6039	,	denotes	,
T1753	6039-6041	NN	denotes	1c
T1754	6041-6042	,	denotes	,
T1747	6042-6044	NN	denotes	1d
T1755	6044-6045	-RRB-	denotes	)
T1756	6045-6046	.	denotes	.
T1757	6046-6238	sentence	denotes	Interestingly however we were unable to recover clones expressing DsRed1 at the same frequency as for the GFP variant reporters after electroporation and selection under identical conditions.
T1758	6047-6060	RB	denotes	Interestingly
T1760	6061-6068	RB	denotes	however
T1761	6069-6071	PRP	denotes	we
T1759	6072-6076	VBD	denotes	were
T1762	6077-6083	JJ	denotes	unable
T1763	6084-6086	TO	denotes	to
T1764	6087-6094	VB	denotes	recover
T1765	6095-6101	NNS	denotes	clones
T1766	6102-6112	VBG	denotes	expressing
T1767	6113-6119	NN	denotes	DsRed1
T1768	6120-6122	IN	denotes	at
T1769	6123-6126	DT	denotes	the
T1771	6127-6131	JJ	denotes	same
T1770	6132-6141	NN	denotes	frequency
T1772	6142-6144	IN	denotes	as
T1773	6145-6148	IN	denotes	for
T1774	6149-6152	DT	denotes	the
T1776	6153-6156	NN	denotes	GFP
T1777	6157-6164	JJ	denotes	variant
T1775	6165-6174	NNS	denotes	reporters
T1778	6175-6180	IN	denotes	after
T1779	6181-6196	NN	denotes	electroporation
T1780	6197-6200	CC	denotes	and
T1781	6201-6210	NN	denotes	selection
T1782	6211-6216	IN	denotes	under
T1783	6217-6226	JJ	denotes	identical
T1784	6227-6237	NNS	denotes	conditions
T1785	6237-6238	.	denotes	.
T1786	6238-6471	sentence	denotes	Indeed the DsRed1 expressing clones that we were able to recover did not retain their characteristic ES cell morphology, usually appearing smaller and more spherical (Fig. 1e and 1f), or occasionally spindle shaped (data not shown).
T1787	6239-6245	RB	denotes	Indeed
T1789	6246-6249	DT	denotes	the
T1791	6250-6256	NN	denotes	DsRed1
T1792	6257-6267	VBG	denotes	expressing
T1790	6268-6274	NNS	denotes	clones
T1793	6275-6279	WDT	denotes	that
T1795	6280-6282	PRP	denotes	we
T1794	6283-6287	VBD	denotes	were
T1796	6288-6292	JJ	denotes	able
T1797	6293-6295	TO	denotes	to
T1798	6296-6303	VB	denotes	recover
T1799	6304-6307	VBD	denotes	did
T1800	6308-6311	RB	denotes	not
T1788	6312-6318	VB	denotes	retain
T1801	6319-6324	PRP$	denotes	their
T1803	6325-6339	JJ	denotes	characteristic
T1804	6340-6342	NN	denotes	ES
T1805	6343-6347	NN	denotes	cell
T1802	6348-6358	NN	denotes	morphology
T1806	6358-6360	,	denotes	,
T1807	6360-6367	RB	denotes	usually
T1808	6368-6377	VBG	denotes	appearing
T1809	6378-6385	JJR	denotes	smaller
T1810	6386-6389	CC	denotes	and
T1811	6390-6394	RBR	denotes	more
T1812	6395-6404	JJ	denotes	spherical
T1813	6405-6406	-LRB-	denotes	(
T1815	6406-6410	NNP	denotes	Fig.
T1814	6411-6413	NN	denotes	1e
T1816	6414-6417	CC	denotes	and
T1817	6418-6420	NN	denotes	1f
T1818	6420-6421	-RRB-	denotes	)
T1819	6421-6423	,	denotes	,
T1820	6423-6425	CC	denotes	or
T1821	6426-6438	RB	denotes	occasionally
T1823	6439-6446	NN	denotes	spindle
T1822	6447-6453	VBN	denotes	shaped
T1824	6454-6455	-LRB-	denotes	(
T1826	6455-6459	NNS	denotes	data
T1827	6460-6463	RB	denotes	not
T1825	6464-6469	VBN	denotes	shown
T1828	6469-6470	-RRB-	denotes	)
T1829	6470-6471	.	denotes	.
T1830	6471-6614	sentence	denotes	Unlike the fluorescence produced by the GFP variants which was evenly distributed throughout the cells, the DsRed signal was never homogenous.
T1831	6472-6478	IN	denotes	Unlike
T1833	6479-6482	DT	denotes	the
T1834	6483-6495	NN	denotes	fluorescence
T1835	6496-6504	VBN	denotes	produced
T1836	6505-6507	IN	denotes	by
T1837	6508-6511	DT	denotes	the
T1839	6512-6515	NN	denotes	GFP
T1838	6516-6524	NNS	denotes	variants
T1840	6525-6530	WDT	denotes	which
T1842	6531-6534	VBD	denotes	was
T1843	6535-6541	RB	denotes	evenly
T1841	6542-6553	VBN	denotes	distributed
T1844	6554-6564	IN	denotes	throughout
T1845	6565-6568	DT	denotes	the
T1846	6569-6574	NNS	denotes	cells
T1847	6574-6576	,	denotes	,
T1848	6576-6579	DT	denotes	the
T1850	6580-6585	NNP	denotes	DsRed
T1849	6586-6592	NN	denotes	signal
T1832	6593-6596	VBD	denotes	was
T1851	6597-6602	RB	denotes	never
T1852	6603-6613	JJ	denotes	homogenous
T1853	6613-6614	.	denotes	.
T1854	6614-6713	sentence	denotes	Red fluorescence was often aggregated in what appeared to be perinuclear regions of the cytoplasm.
T1855	6615-6618	JJ	denotes	Red
T1856	6619-6631	NN	denotes	fluorescence
T1858	6632-6635	VBD	denotes	was
T1859	6636-6641	RB	denotes	often
T1857	6642-6652	VBN	denotes	aggregated
T1860	6653-6655	IN	denotes	in
T1861	6656-6660	WP	denotes	what
T1862	6661-6669	VBD	denotes	appeared
T1863	6670-6672	TO	denotes	to
T1864	6673-6675	VB	denotes	be
T1865	6676-6687	JJ	denotes	perinuclear
T1866	6688-6695	NNS	denotes	regions
T1867	6696-6698	IN	denotes	of
T1868	6699-6702	DT	denotes	the
T1869	6703-6712	NN	denotes	cytoplasm
T1870	6712-6713	.	denotes	.
T1871	6713-6890	sentence	denotes	Similar observations have been made by Furuta and colleagues when they used recombinant sindbis virus for the labeling of dendrites and axons within the CNS of adult mice [10].
T1872	6714-6721	JJ	denotes	Similar
T1873	6722-6734	NNS	denotes	observations
T1875	6735-6739	VBP	denotes	have
T1876	6740-6744	VBN	denotes	been
T1874	6745-6749	VBN	denotes	made
T1877	6750-6752	IN	denotes	by
T1878	6753-6759	NNP	denotes	Furuta
T1879	6760-6763	CC	denotes	and
T1880	6764-6774	NNS	denotes	colleagues
T1881	6775-6779	WRB	denotes	when
T1883	6780-6784	PRP	denotes	they
T1882	6785-6789	VBD	denotes	used
T1884	6790-6801	JJ	denotes	recombinant
T1886	6802-6809	NN	denotes	sindbis
T1885	6810-6815	NN	denotes	virus
T1887	6816-6819	IN	denotes	for
T1888	6820-6823	DT	denotes	the
T1889	6824-6832	NN	denotes	labeling
T1890	6833-6835	IN	denotes	of
T1891	6836-6845	NNS	denotes	dendrites
T1892	6846-6849	CC	denotes	and
T1893	6850-6855	NNS	denotes	axons
T1894	6856-6862	IN	denotes	within
T1895	6863-6866	DT	denotes	the
T1896	6867-6870	NN	denotes	CNS
T1897	6871-6873	IN	denotes	of
T1898	6874-6879	JJ	denotes	adult
T1899	6880-6884	NNS	denotes	mice
T1900	6885-6886	-LRB-	denotes	[
T1901	6886-6888	CD	denotes	10
T1902	6888-6889	-RRB-	denotes	]
T1903	6889-6890	.	denotes	.
T1904	6890-7079	sentence	denotes	In order to generate a DsRed variant that may be amenable to use in ES cells and mice, we recently introduced various sequences that direct nuclear localization to the DsRed coding region.
T1905	6891-6893	IN	denotes	In
T1907	6894-6899	NN	denotes	order
T1908	6900-6902	TO	denotes	to
T1909	6903-6911	VB	denotes	generate
T1910	6912-6913	DT	denotes	a
T1912	6914-6919	NNP	denotes	DsRed
T1911	6920-6927	NN	denotes	variant
T1913	6928-6932	WDT	denotes	that
T1915	6933-6936	MD	denotes	may
T1914	6937-6939	VB	denotes	be
T1916	6940-6948	JJ	denotes	amenable
T1917	6949-6951	IN	denotes	to
T1918	6952-6955	NN	denotes	use
T1919	6956-6958	IN	denotes	in
T1920	6959-6961	NN	denotes	ES
T1921	6962-6967	NNS	denotes	cells
T1922	6968-6971	CC	denotes	and
T1923	6972-6976	NNS	denotes	mice
T1924	6976-6978	,	denotes	,
T1925	6978-6980	PRP	denotes	we
T1926	6981-6989	RB	denotes	recently
T1906	6990-7000	VBD	denotes	introduced
T1927	7001-7008	JJ	denotes	various
T1928	7009-7018	NNS	denotes	sequences
T1929	7019-7023	WDT	denotes	that
T1930	7024-7030	VBP	denotes	direct
T1931	7031-7038	JJ	denotes	nuclear
T1932	7039-7051	NN	denotes	localization
T1933	7052-7054	IN	denotes	to
T1934	7055-7058	DT	denotes	the
T1936	7059-7064	NNP	denotes	DsRed
T1937	7065-7071	VBG	denotes	coding
T1935	7072-7078	NN	denotes	region
T1938	7078-7079	.	denotes	.
T1939	7079-7243	sentence	denotes	However, again we were unable to generate ES cells or mice exhibiting robust widespread reporter activity (A.-K.H. and V.E. Papaioannou, unpublished observations).
T1940	7080-7087	RB	denotes	However
T1942	7087-7089	,	denotes	,
T1943	7089-7094	RB	denotes	again
T1944	7095-7097	PRP	denotes	we
T1941	7098-7102	VBD	denotes	were
T1945	7103-7109	JJ	denotes	unable
T1946	7110-7112	TO	denotes	to
T1947	7113-7121	VB	denotes	generate
T1948	7122-7124	NN	denotes	ES
T1949	7125-7130	NNS	denotes	cells
T1950	7131-7133	CC	denotes	or
T1951	7134-7138	NNS	denotes	mice
T1952	7139-7149	VBG	denotes	exhibiting
T1953	7150-7156	JJ	denotes	robust
T1955	7157-7167	JJ	denotes	widespread
T1956	7168-7176	NN	denotes	reporter
T1954	7177-7185	NN	denotes	activity
T1957	7186-7187	-LRB-	denotes	(
T1959	7187-7189	NNP	denotes	A.
T1961	7189-7190	HYPH	denotes	-
T1960	7190-7194	NNP	denotes	K.H.
T1962	7195-7198	CC	denotes	and
T1963	7199-7203	NNP	denotes	V.E.
T1958	7204-7215	NNP	denotes	Papaioannou
T1964	7215-7217	,	denotes	,
T1965	7217-7228	JJ	denotes	unpublished
T1966	7229-7241	NNS	denotes	observations
T1967	7241-7242	-RRB-	denotes	)
T1968	7242-7243	.	denotes	.
T1969	7243-7244	sentence	denotes
T5231	7254-7266	JJ	denotes	Non-invasive
T5233	7267-7275	JJ	denotes	multiple
T5234	7276-7284	NN	denotes	reporter
T5232	7285-7298	NN	denotes	visualization
T5235	7299-7301	IN	denotes	in
T5236	7302-7304	NN	denotes	ES
T5237	7305-7310	NNS	denotes	cells
T5238	7310-7311	.	denotes	.
T5239	7311-7393	sentence	denotes	(a-d) a mixture of CK6/ECFP (ECFP+) and YC5/EYFP (EYFP+) ES cells at low density.
T5240	7312-7313	-LRB-	denotes	(
T5241	7313-7314	LS	denotes	a
T5243	7314-7315	SYM	denotes	-
T5244	7315-7316	LS	denotes	d
T5245	7316-7317	-RRB-	denotes	)
T5246	7318-7319	DT	denotes	a
T5242	7320-7327	NN	denotes	mixture
T5247	7328-7330	IN	denotes	of
T5248	7331-7334	NN	denotes	CK6
T5250	7334-7335	HYPH	denotes	/
T5249	7335-7339	NN	denotes	ECFP
T5252	7340-7341	-LRB-	denotes	(
T5253	7341-7346	NN	denotes	ECFP+
T5254	7346-7347	-RRB-	denotes	)
T5255	7348-7351	CC	denotes	and
T5256	7352-7355	NN	denotes	YC5
T5258	7355-7356	HYPH	denotes	/
T5257	7356-7360	NN	denotes	EYFP
T5259	7361-7362	-LRB-	denotes	(
T5260	7362-7367	NN	denotes	EYFP+
T5261	7367-7368	-RRB-	denotes	)
T5262	7369-7371	NN	denotes	ES
T5251	7372-7377	NNS	denotes	cells
T5263	7378-7380	IN	denotes	at
T5264	7381-7384	JJ	denotes	low
T5265	7385-7392	NN	denotes	density
T5266	7392-7393	.	denotes	.
T5267	7393-7453	sentence	denotes	(a) image taken under bright field with no epifluorescence.
T5268	7394-7395	-LRB-	denotes	(
T5269	7395-7396	LS	denotes	a
T5271	7396-7397	-RRB-	denotes	)
T5270	7398-7403	NN	denotes	image
T5272	7404-7409	VBN	denotes	taken
T5273	7410-7415	IN	denotes	under
T5274	7416-7422	JJ	denotes	bright
T5275	7423-7428	NN	denotes	field
T5276	7429-7433	IN	denotes	with
T5277	7434-7436	DT	denotes	no
T5278	7437-7452	NN	denotes	epifluorescence
T5279	7452-7453	.	denotes	.
T5280	7453-7504	sentence	denotes	(b) dark field image taken through an EYFP filter.
T5281	7454-7455	-LRB-	denotes	(
T5282	7455-7456	LS	denotes	b
T5284	7456-7457	-RRB-	denotes	)
T5285	7458-7462	JJ	denotes	dark
T5286	7463-7468	NN	denotes	field
T5283	7469-7474	NN	denotes	image
T5287	7475-7480	VBN	denotes	taken
T5288	7481-7488	IN	denotes	through
T5289	7489-7491	DT	denotes	an
T5291	7492-7496	NN	denotes	EYFP
T5290	7497-7503	NN	denotes	filter
T5292	7503-7504	.	denotes	.
T5293	7504-7555	sentence	denotes	(c) dark field image taken through an ECFP filter.
T5294	7505-7506	-LRB-	denotes	(
T5295	7506-7507	LS	denotes	c
T5297	7507-7508	-RRB-	denotes	)
T5298	7509-7513	VB	denotes	dark
T5299	7514-7519	NN	denotes	field
T5296	7520-7525	NN	denotes	image
T5300	7526-7531	VBN	denotes	taken
T5301	7532-7539	IN	denotes	through
T5302	7540-7542	DT	denotes	an
T5304	7543-7547	NN	denotes	ECFP
T5303	7548-7554	NN	denotes	filter
T5305	7554-7555	.	denotes	.
T5306	7555-7647	sentence	denotes	(d) dark field double exposure image acquired by consecutively using ECFP and EYFP filters.
T5307	7556-7557	-LRB-	denotes	(
T5308	7557-7558	LS	denotes	d
T5310	7558-7559	-RRB-	denotes	)
T5311	7560-7564	JJ	denotes	dark
T5312	7565-7570	NN	denotes	field
T5313	7571-7577	JJ	denotes	double
T5314	7578-7586	NN	denotes	exposure
T5309	7587-7592	NN	denotes	image
T5315	7593-7601	VBN	denotes	acquired
T5316	7602-7604	IN	denotes	by
T5317	7605-7618	RB	denotes	consecutively
T5318	7619-7624	VBG	denotes	using
T5319	7625-7629	NN	denotes	ECFP
T5321	7630-7633	CC	denotes	and
T5322	7634-7638	NN	denotes	EYFP
T5320	7639-7646	NNS	denotes	filters
T5323	7646-7647	.	denotes	.
T5324	7647-7702	sentence	denotes	ECFP+ cells can clearly be discerned from EYFP+ cells.
T5325	7648-7653	JJ	denotes	ECFP+
T5326	7654-7659	NNS	denotes	cells
T5328	7660-7663	MD	denotes	can
T5329	7664-7671	RB	denotes	clearly
T5330	7672-7674	VB	denotes	be
T5327	7675-7684	VBN	denotes	discerned
T5331	7685-7689	IN	denotes	from
T5332	7690-7695	JJ	denotes	EYFP+
T5333	7696-7701	NNS	denotes	cells
T5334	7701-7702	.	denotes	.
T5335	7702-7789	sentence	denotes	(e-f) culture of mixed populations of ES cells comprising ECFP+, EYFP+ and RFP+ cells.
T5336	7703-7704	-LRB-	denotes	(
T5337	7704-7705	LS	denotes	e
T5339	7705-7706	SYM	denotes	-
T5340	7706-7707	LS	denotes	f
T5341	7707-7708	-RRB-	denotes	)
T5338	7709-7716	NN	denotes	culture
T5342	7717-7719	IN	denotes	of
T5343	7720-7725	VBN	denotes	mixed
T5344	7726-7737	NNS	denotes	populations
T5345	7738-7740	IN	denotes	of
T5346	7741-7743	NN	denotes	ES
T5347	7744-7749	NNS	denotes	cells
T5348	7750-7760	VBG	denotes	comprising
T5349	7761-7766	JJ	denotes	ECFP+
T5351	7766-7768	,	denotes	,
T5352	7768-7773	JJ	denotes	EYFP+
T5353	7774-7777	CC	denotes	and
T5354	7778-7782	JJ	denotes	RFP+
T5350	7783-7788	NNS	denotes	cells
T5355	7788-7789	.	denotes	.
T5356	7789-7818	sentence	denotes	Scale bars represent 100 μm.
T5357	7790-7795	NN	denotes	Scale
T5358	7796-7800	NNS	denotes	bars
T5359	7801-7810	VBP	denotes	represent
T5360	7811-7814	CD	denotes	100
T5361	7815-7817	NNS	denotes	μm
T5362	7817-7818	.	denotes	.
T1971	7819-7822	CD	denotes	Two
T1970	7819-7969	sentence	denotes	Two ECFP+ and two EYFP+ clones exhibiting robust fluorescence were used to generate chimeras through ES cell <-> morula stage embryo aggregation [13].
T1972	7823-7828	JJ	denotes	ECFP+
T1974	7829-7832	CC	denotes	and
T1975	7833-7836	CD	denotes	two
T1976	7837-7842	JJ	denotes	EYFP+
T1973	7843-7849	NNS	denotes	clones
T1978	7850-7860	VBG	denotes	exhibiting
T1979	7861-7867	JJ	denotes	robust
T1980	7868-7880	NN	denotes	fluorescence
T1981	7881-7885	VBD	denotes	were
T1977	7886-7890	VBN	denotes	used
T1982	7891-7893	TO	denotes	to
T1983	7894-7902	VB	denotes	generate
T1984	7903-7911	NNS	denotes	chimeras
T1985	7912-7919	IN	denotes	through
T1986	7920-7922	NN	denotes	ES
T1987	7923-7927	NN	denotes	cell
T1988	7928-7929	SYM	denotes	<
T1990	7929-7930	HYPH	denotes	-
T1991	7930-7931	SYM	denotes	>
T1992	7932-7938	NN	denotes	morula
T1993	7939-7944	NN	denotes	stage
T1994	7945-7951	NN	denotes	embryo
T1989	7952-7963	NN	denotes	aggregation
T1995	7964-7965	-LRB-	denotes	[
T1996	7965-7967	CD	denotes	13
T1997	7967-7968	-RRB-	denotes	]
T1998	7968-7969	.	denotes	.
T1999	7969-8242	sentence	denotes	All four ES cell lines transmitted through the germline, leading to the establishment of, CK4/ECFP and CK6/ECFP, two strains of mice having cyan fluorescent protein expression and, YC5/EYFP and 2A4/EYFP, two strains having widespread yellow fluorescent protein expression.
T2000	7970-7973	DT	denotes	All
T2002	7974-7978	CD	denotes	four
T2003	7979-7981	NN	denotes	ES
T2004	7982-7986	NN	denotes	cell
T2001	7987-7992	NNS	denotes	lines
T2005	7993-8004	VBD	denotes	transmitted
T2006	8005-8012	IN	denotes	through
T2007	8013-8016	DT	denotes	the
T2008	8017-8025	NN	denotes	germline
T2009	8025-8027	,	denotes	,
T2010	8027-8034	VBG	denotes	leading
T2011	8035-8037	IN	denotes	to
T2012	8038-8041	DT	denotes	the
T2013	8042-8055	NN	denotes	establishment
T2014	8056-8058	IN	denotes	of
T2015	8058-8060	,	denotes	,
T2016	8060-8063	NN	denotes	CK4
T2018	8063-8064	HYPH	denotes	/
T2017	8064-8068	NN	denotes	ECFP
T2019	8069-8072	CC	denotes	and
T2020	8073-8076	NN	denotes	CK6
T2022	8076-8077	HYPH	denotes	/
T2021	8077-8081	NN	denotes	ECFP
T2023	8081-8083	,	denotes	,
T2024	8083-8086	CD	denotes	two
T2025	8087-8094	NNS	denotes	strains
T2026	8095-8097	IN	denotes	of
T2027	8098-8102	NNS	denotes	mice
T2028	8103-8109	VBG	denotes	having
T2029	8110-8114	NN	denotes	cyan
T2031	8115-8126	JJ	denotes	fluorescent
T2030	8127-8134	NN	denotes	protein
T2032	8135-8145	NN	denotes	expression
T2033	8146-8149	CC	denotes	and
T2034	8149-8151	,	denotes	,
T2035	8151-8154	NN	denotes	YC5
T2037	8154-8155	HYPH	denotes	/
T2036	8155-8159	NN	denotes	EYFP
T2038	8160-8163	CC	denotes	and
T2039	8164-8167	NN	denotes	2A4
T2041	8167-8168	HYPH	denotes	/
T2040	8168-8172	NN	denotes	EYFP
T2042	8172-8174	,	denotes	,
T2043	8174-8177	CD	denotes	two
T2044	8178-8185	NNS	denotes	strains
T2045	8186-8192	VBG	denotes	having
T2046	8193-8203	JJ	denotes	widespread
T2048	8204-8210	JJ	denotes	yellow
T2050	8211-8222	JJ	denotes	fluorescent
T2049	8223-8230	NN	denotes	protein
T2047	8231-8241	NN	denotes	expression
T2051	8241-8242	.	denotes	.
T2052	8242-8415	sentence	denotes	In these mice the respective FP expression is constituitive, starting at preimplantation stages and continuing through postimplantation embryogenesis to adulthood (Fig. 2).
T2053	8243-8245	IN	denotes	In
T2055	8246-8251	DT	denotes	these
T2056	8252-8256	NNS	denotes	mice
T2057	8257-8260	DT	denotes	the
T2059	8261-8271	JJ	denotes	respective
T2060	8272-8274	NN	denotes	FP
T2058	8275-8285	NN	denotes	expression
T2054	8286-8288	VBZ	denotes	is
T2061	8289-8302	JJ	denotes	constituitive
T2062	8302-8304	,	denotes	,
T2063	8304-8312	VBG	denotes	starting
T2064	8313-8315	IN	denotes	at
T2065	8316-8331	NN	denotes	preimplantation
T2066	8332-8338	NNS	denotes	stages
T2067	8339-8342	CC	denotes	and
T2068	8343-8353	VBG	denotes	continuing
T2069	8354-8361	IN	denotes	through
T2070	8362-8378	NN	denotes	postimplantation
T2071	8379-8392	NN	denotes	embryogenesis
T2072	8393-8395	IN	denotes	to
T2073	8396-8405	NN	denotes	adulthood
T2074	8406-8407	-LRB-	denotes	(
T2075	8407-8411	NN	denotes	Fig.
T2076	8412-8413	CD	denotes	2
T2077	8413-8414	-RRB-	denotes	)
T2078	8414-8415	.	denotes	.
T2079	8415-8508	sentence	denotes	One line expressing each color was chosen for further analysis and breeding to homozygosity.
T2080	8416-8419	CD	denotes	One
T2081	8420-8424	NN	denotes	line
T2083	8425-8435	VBG	denotes	expressing
T2084	8436-8440	DT	denotes	each
T2085	8441-8446	NN	denotes	color
T2086	8447-8450	VBD	denotes	was
T2082	8451-8457	VBN	denotes	chosen
T2087	8458-8461	IN	denotes	for
T2088	8462-8469	JJ	denotes	further
T2089	8470-8478	NN	denotes	analysis
T2090	8479-8482	CC	denotes	and
T2091	8483-8491	NN	denotes	breeding
T2092	8492-8494	IN	denotes	to
T2093	8495-8507	NN	denotes	homozygosity
T2094	8507-8508	.	denotes	.
T2095	8508-8663	sentence	denotes	The availability of homozygous animals demonstrated that both reporter gene expression and the site of transgene integration were developmentally neutral.
T2096	8509-8512	DT	denotes	The
T2097	8513-8525	NN	denotes	availability
T2099	8526-8528	IN	denotes	of
T2100	8529-8539	JJ	denotes	homozygous
T2101	8540-8547	NNS	denotes	animals
T2098	8548-8560	VBD	denotes	demonstrated
T2102	8561-8565	IN	denotes	that
T2104	8566-8570	CC	denotes	both
T2106	8571-8579	NN	denotes	reporter
T2107	8580-8584	NN	denotes	gene
T2105	8585-8595	NN	denotes	expression
T2108	8596-8599	CC	denotes	and
T2109	8600-8603	DT	denotes	the
T2110	8604-8608	NN	denotes	site
T2111	8609-8611	IN	denotes	of
T2112	8612-8621	NN	denotes	transgene
T2113	8622-8633	NN	denotes	integration
T2103	8634-8638	VBD	denotes	were
T2114	8639-8654	RB	denotes	developmentally
T2115	8655-8662	JJ	denotes	neutral
T2116	8662-8663	.	denotes	.
T2117	8663-8664	sentence	denotes
T5432	8674-8678	JJ	denotes	Dual
T5434	8679-8691	JJ	denotes	non-invasive
T5435	8692-8700	NN	denotes	reporter
T5433	8701-8714	NN	denotes	visualization
T5436	8715-8717	IN	denotes	in
T5437	8718-8725	NNS	denotes	embryos
T5438	8726-8729	CC	denotes	and
T5439	8730-8735	JJ	denotes	adult
T5440	8736-8740	NNS	denotes	mice
T5441	8740-8741	.	denotes	.
T5442	8741-8823	sentence	denotes	Transgenic animals are hemizygous for either the CK6/ECFP or YC5/EYFP transgenes.
T5443	8742-8752	JJ	denotes	Transgenic
T5444	8753-8760	NNS	denotes	animals
T5445	8761-8764	VBP	denotes	are
T5446	8765-8775	JJ	denotes	hemizygous
T5447	8776-8779	IN	denotes	for
T5448	8780-8786	CC	denotes	either
T5450	8787-8790	DT	denotes	the
T5451	8791-8794	NN	denotes	CK6
T5453	8794-8795	HYPH	denotes	/
T5452	8795-8799	NN	denotes	ECFP
T5454	8800-8802	CC	denotes	or
T5455	8803-8806	NN	denotes	YC5
T5457	8806-8807	HYPH	denotes	/
T5456	8807-8811	NN	denotes	EYFP
T5449	8812-8822	NNS	denotes	transgenes
T5458	8822-8823	.	denotes	.
T5459	8823-8910	sentence	denotes	(a-d) blastocyst stage (E3.5) embryos, that are either fluorescent or non-fluorescent.
T5460	8824-8825	-LRB-	denotes	(
T5461	8825-8826	LS	denotes	a
T5463	8826-8827	SYM	denotes	-
T5464	8827-8828	LS	denotes	d
T5465	8828-8829	-RRB-	denotes	)
T5466	8830-8840	NN	denotes	blastocyst
T5467	8841-8846	NN	denotes	stage
T5468	8847-8848	-LRB-	denotes	(
T5469	8848-8852	NN	denotes	E3.5
T5470	8852-8853	-RRB-	denotes	)
T5462	8854-8861	NNS	denotes	embryos
T5471	8861-8863	,	denotes	,
T5472	8863-8867	WDT	denotes	that
T5473	8868-8871	VBP	denotes	are
T5474	8872-8878	DT	denotes	either
T5475	8879-8890	JJ	denotes	fluorescent
T5476	8891-8893	CC	denotes	or
T5477	8894-8909	JJ	denotes	non-fluorescent
T5478	8909-8910	.	denotes	.
T5479	8910-9054	sentence	denotes	Fluorescent embryos are either blue/cyan or yellow/green, thereby being hemizygous for either the CK6/ECFP or YC5/EYFP transgenes respectively.
T5480	8911-8922	JJ	denotes	Fluorescent
T5481	8923-8930	NNS	denotes	embryos
T5482	8931-8934	VBP	denotes	are
T5483	8935-8941	CC	denotes	either
T5485	8942-8946	JJ	denotes	blue
T5486	8946-8947	HYPH	denotes	/
T5484	8947-8951	JJ	denotes	cyan
T5487	8952-8954	CC	denotes	or
T5488	8955-8961	JJ	denotes	yellow
T5490	8961-8962	HYPH	denotes	/
T5489	8962-8967	JJ	denotes	green
T5491	8967-8969	,	denotes	,
T5492	8969-8976	RB	denotes	thereby
T5493	8977-8982	VBG	denotes	being
T5494	8983-8993	JJ	denotes	hemizygous
T5495	8994-8997	IN	denotes	for
T5496	8998-9004	CC	denotes	either
T5498	9005-9008	DT	denotes	the
T5499	9009-9012	NN	denotes	CK6
T5500	9012-9013	HYPH	denotes	/
T5497	9013-9017	NN	denotes	ECFP
T5502	9018-9020	CC	denotes	or
T5503	9021-9024	NN	denotes	YC5
T5505	9024-9025	HYPH	denotes	/
T5504	9025-9029	NN	denotes	EYFP
T5501	9030-9040	NNS	denotes	transgenes
T5506	9041-9053	RB	denotes	respectively
T5507	9053-9054	.	denotes	.
T5508	9054-9155	sentence	denotes	Non-fluorescent embryos (marked with an asterix on panel a) are of the wild type outbred ICR strain.
T5509	9055-9070	JJ	denotes	Non-fluorescent
T5510	9071-9078	NNS	denotes	embryos
T5512	9079-9080	-LRB-	denotes	(
T5513	9080-9086	VBN	denotes	marked
T5514	9087-9091	IN	denotes	with
T5515	9092-9094	DT	denotes	an
T5516	9095-9102	NN	denotes	asterix
T5517	9103-9105	IN	denotes	on
T5518	9106-9111	NN	denotes	panel
T5519	9112-9113	NN	denotes	a
T5520	9113-9114	-RRB-	denotes	)
T5511	9115-9118	VBP	denotes	are
T5521	9119-9121	IN	denotes	of
T5522	9122-9125	DT	denotes	the
T5524	9126-9130	JJ	denotes	wild
T5525	9131-9135	NN	denotes	type
T5526	9136-9143	VBN	denotes	outbred
T5527	9144-9147	NN	denotes	ICR
T5523	9148-9154	NN	denotes	strain
T5528	9154-9155	.	denotes	.
T5529	9155-9215	sentence	denotes	(a) image taken under bright field with no epifluorescence.
T5530	9156-9157	-LRB-	denotes	(
T5531	9157-9158	LS	denotes	a
T5533	9158-9159	-RRB-	denotes	)
T5532	9160-9165	NN	denotes	image
T5534	9166-9171	VBN	denotes	taken
T5535	9172-9177	IN	denotes	under
T5536	9178-9184	JJ	denotes	bright
T5537	9185-9190	NN	denotes	field
T5538	9191-9195	IN	denotes	with
T5539	9196-9198	DT	denotes	no
T5540	9199-9214	NN	denotes	epifluorescence
T5541	9214-9215	.	denotes	.
T5542	9215-9266	sentence	denotes	(b) dark field image taken through an EYFP filter.
T5543	9216-9217	-LRB-	denotes	(
T5544	9217-9218	LS	denotes	b
T5546	9218-9219	-RRB-	denotes	)
T5547	9220-9224	JJ	denotes	dark
T5548	9225-9230	NN	denotes	field
T5545	9231-9236	NN	denotes	image
T5549	9237-9242	VBN	denotes	taken
T5550	9243-9250	IN	denotes	through
T5551	9251-9253	DT	denotes	an
T5553	9254-9258	NN	denotes	EYFP
T5552	9259-9265	NN	denotes	filter
T5554	9265-9266	.	denotes	.
T5555	9266-9317	sentence	denotes	(c) dark field image taken through an ECFP filter.
T5556	9267-9268	-LRB-	denotes	(
T5557	9268-9269	LS	denotes	c
T5559	9269-9270	-RRB-	denotes	)
T5560	9271-9275	VB	denotes	dark
T5561	9276-9281	NN	denotes	field
T5558	9282-9287	NN	denotes	image
T5562	9288-9293	VBN	denotes	taken
T5563	9294-9301	IN	denotes	through
T5564	9302-9304	DT	denotes	an
T5566	9305-9309	NN	denotes	ECFP
T5565	9310-9316	NN	denotes	filter
T5567	9316-9317	.	denotes	.
T5568	9317-9409	sentence	denotes	(d) dark field double exposure image acquired by consecutively using ECFP and EYFP filters.
T5569	9318-9319	-LRB-	denotes	(
T5570	9319-9320	LS	denotes	d
T5572	9320-9321	-RRB-	denotes	)
T5573	9322-9326	JJ	denotes	dark
T5574	9327-9332	NN	denotes	field
T5575	9333-9339	JJ	denotes	double
T5576	9340-9348	NN	denotes	exposure
T5571	9349-9354	NN	denotes	image
T5577	9355-9363	VBN	denotes	acquired
T5578	9364-9366	IN	denotes	by
T5579	9367-9380	RB	denotes	consecutively
T5580	9381-9386	VBG	denotes	using
T5581	9387-9391	NN	denotes	ECFP
T5583	9392-9395	CC	denotes	and
T5584	9396-9400	NN	denotes	EYFP
T5582	9401-9408	NNS	denotes	filters
T5585	9408-9409	.	denotes	.
T5586	9409-9631	sentence	denotes	(e-h) two transgenic (TG/+) E13.5 embryos dissected free of their extraembryonic membranes, one is blue/cyan fluorescent (CK6/ECFP transgenic, left) and the other is yellow/green fluorescent (7YC5/EYFP transgenic, right).
T5587	9410-9411	-LRB-	denotes	(
T5588	9411-9412	LS	denotes	e
T5590	9412-9413	SYM	denotes	-
T5591	9413-9414	LS	denotes	h
T5592	9414-9415	-RRB-	denotes	)
T5593	9416-9419	CD	denotes	two
T5595	9420-9430	JJ	denotes	transgenic
T5596	9431-9432	-LRB-	denotes	(
T5597	9432-9434	NN	denotes	TG
T5598	9434-9435	HYPH	denotes	/
T5599	9435-9436	SYM	denotes	+
T5600	9436-9437	-RRB-	denotes	)
T5601	9438-9443	NN	denotes	E13.5
T5594	9444-9451	NNS	denotes	embryos
T5602	9452-9461	VBN	denotes	dissected
T5603	9462-9466	JJ	denotes	free
T5604	9467-9469	IN	denotes	of
T5605	9470-9475	PRP$	denotes	their
T5607	9476-9490	JJ	denotes	extraembryonic
T5606	9491-9500	NNS	denotes	membranes
T5608	9500-9502	,	denotes	,
T5609	9502-9505	CD	denotes	one
T5589	9506-9508	VBZ	denotes	is
T5610	9509-9513	JJ	denotes	blue
T5612	9513-9514	HYPH	denotes	/
T5611	9514-9518	JJ	denotes	cyan
T5613	9519-9530	JJ	denotes	fluorescent
T5614	9531-9532	-LRB-	denotes	(
T5616	9532-9535	NN	denotes	CK6
T5618	9535-9536	HYPH	denotes	/
T5617	9536-9540	NN	denotes	ECFP
T5619	9541-9551	JJ	denotes	transgenic
T5620	9551-9553	,	denotes	,
T5615	9553-9557	JJ	denotes	left
T5621	9557-9558	-RRB-	denotes	)
T5622	9559-9562	CC	denotes	and
T5623	9563-9566	DT	denotes	the
T5624	9567-9572	JJ	denotes	other
T5625	9573-9575	VBZ	denotes	is
T5626	9576-9582	JJ	denotes	yellow
T5628	9582-9583	HYPH	denotes	/
T5627	9583-9588	JJ	denotes	green
T5629	9589-9600	JJ	denotes	fluorescent
T5630	9601-9602	-LRB-	denotes	(
T5632	9602-9606	NN	denotes	7YC5
T5634	9606-9607	HYPH	denotes	/
T5633	9607-9611	NN	denotes	EYFP
T5635	9612-9622	JJ	denotes	transgenic
T5636	9622-9624	,	denotes	,
T5631	9624-9629	JJ	denotes	right
T5637	9629-9630	-RRB-	denotes	)
T5638	9630-9631	.	denotes	.
T5639	9631-9691	sentence	denotes	(e) image taken under bright field with no epifluorescence.
T5640	9632-9633	-LRB-	denotes	(
T5641	9633-9634	LS	denotes	e
T5643	9634-9635	-RRB-	denotes	)
T5642	9636-9641	NN	denotes	image
T5644	9642-9647	VBN	denotes	taken
T5645	9648-9653	IN	denotes	under
T5646	9654-9660	JJ	denotes	bright
T5647	9661-9666	NN	denotes	field
T5648	9667-9671	IN	denotes	with
T5649	9672-9674	DT	denotes	no
T5650	9675-9690	NN	denotes	epifluorescence
T5651	9690-9691	.	denotes	.
T5652	9691-9742	sentence	denotes	(f) dark field image taken through an EYFP filter.
T5653	9692-9693	-LRB-	denotes	(
T5654	9693-9694	LS	denotes	f
T5656	9694-9695	-RRB-	denotes	)
T5657	9696-9700	JJ	denotes	dark
T5658	9701-9706	NN	denotes	field
T5655	9707-9712	NN	denotes	image
T5659	9713-9718	VBN	denotes	taken
T5660	9719-9726	IN	denotes	through
T5661	9727-9729	DT	denotes	an
T5663	9730-9734	NN	denotes	EYFP
T5662	9735-9741	NN	denotes	filter
T5664	9741-9742	.	denotes	.
T5665	9742-9794	sentence	denotes	(g), dark field image taken through an ECFP filter.
T5666	9743-9744	-LRB-	denotes	(
T5667	9744-9745	LS	denotes	g
T5669	9745-9746	-RRB-	denotes	)
T5670	9746-9748	,	denotes	,
T5671	9748-9752	JJ	denotes	dark
T5672	9753-9758	NN	denotes	field
T5668	9759-9764	NN	denotes	image
T5673	9765-9770	VBN	denotes	taken
T5674	9771-9778	IN	denotes	through
T5675	9779-9781	DT	denotes	an
T5677	9782-9786	NN	denotes	ECFP
T5676	9787-9793	NN	denotes	filter
T5678	9793-9794	.	denotes	.
T5679	9794-9869	sentence	denotes	(h) double exposure acquired by consecutively using ECFP and EYFP filters.
T5680	9795-9796	-LRB-	denotes	(
T5681	9796-9797	LS	denotes	h
T5683	9797-9798	-RRB-	denotes	)
T5684	9799-9805	JJ	denotes	double
T5682	9806-9814	NN	denotes	exposure
T5685	9815-9823	VBN	denotes	acquired
T5686	9824-9826	IN	denotes	by
T5687	9827-9840	RB	denotes	consecutively
T5688	9841-9846	VBG	denotes	using
T5689	9847-9851	NN	denotes	ECFP
T5691	9852-9855	CC	denotes	and
T5692	9856-9860	NN	denotes	EYFP
T5690	9861-9868	NNS	denotes	filters
T5693	9868-9869	.	denotes	.
T5694	9869-9972	sentence	denotes	(i-k) two three week old transgenic mice each exclusively expressing either the ECFP or EYFP reporter.
T5695	9870-9871	-LRB-	denotes	(
T5696	9871-9872	LS	denotes	i
T5698	9872-9873	SYM	denotes	-
T5699	9873-9874	LS	denotes	k
T5700	9874-9875	-RRB-	denotes	)
T5701	9876-9879	CD	denotes	two
T5702	9880-9885	CD	denotes	three
T5703	9886-9890	NN	denotes	week
T5704	9891-9894	JJ	denotes	old
T5705	9895-9905	JJ	denotes	transgenic
T5697	9906-9910	NNS	denotes	mice
T5706	9911-9915	DT	denotes	each
T5707	9916-9927	RB	denotes	exclusively
T5708	9928-9938	VBG	denotes	expressing
T5709	9939-9945	CC	denotes	either
T5711	9946-9949	DT	denotes	the
T5710	9950-9954	NN	denotes	ECFP
T5713	9955-9957	CC	denotes	or
T5714	9958-9962	NN	denotes	EYFP
T5712	9963-9971	NN	denotes	reporter
T5715	9971-9972	.	denotes	.
T5716	9972-10093	sentence	denotes	All images shown are double exposures taken by consecutively using ECFP and then the EYFP filters under epifluorescence.
T5717	9973-9976	DT	denotes	All
T5718	9977-9983	NNS	denotes	images
T5720	9984-9989	VBN	denotes	shown
T5719	9990-9993	VBP	denotes	are
T5721	9994-10000	JJ	denotes	double
T5722	10001-10010	NNS	denotes	exposures
T5723	10011-10016	VBN	denotes	taken
T5724	10017-10019	IN	denotes	by
T5725	10020-10033	RB	denotes	consecutively
T5726	10034-10039	VBG	denotes	using
T5727	10040-10044	NN	denotes	ECFP
T5728	10045-10048	CC	denotes	and
T5729	10049-10053	RB	denotes	then
T5730	10054-10057	DT	denotes	the
T5731	10058-10062	NN	denotes	EYFP
T5732	10063-10070	NNS	denotes	filters
T5733	10071-10076	IN	denotes	under
T5734	10077-10092	NN	denotes	epifluorescence
T5735	10092-10093	.	denotes	.
T5736	10093-10179	sentence	denotes	(i) CK6/ECFP (TG/+) transgenic mouse bottom, YC5/EYFP (TG/+) transgenic mouse bottom.
T5737	10094-10095	-LRB-	denotes	(
T5738	10095-10096	LS	denotes	i
T5740	10096-10097	-RRB-	denotes	)
T5741	10098-10101	NN	denotes	CK6
T5743	10101-10102	HYPH	denotes	/
T5742	10102-10106	NN	denotes	ECFP
T5744	10107-10108	-LRB-	denotes	(
T5745	10108-10110	NN	denotes	TG
T5746	10110-10111	HYPH	denotes	/
T5747	10111-10112	SYM	denotes	+
T5748	10112-10113	-RRB-	denotes	)
T5749	10114-10124	JJ	denotes	transgenic
T5739	10125-10130	NN	denotes	mouse
T5750	10131-10137	NN	denotes	bottom
T5751	10137-10139	,	denotes	,
T5752	10139-10142	NN	denotes	YC5
T5754	10142-10143	HYPH	denotes	/
T5753	10143-10147	NN	denotes	EYFP
T5756	10148-10149	-LRB-	denotes	(
T5757	10149-10151	NN	denotes	TG
T5758	10151-10152	HYPH	denotes	/
T5759	10152-10153	SYM	denotes	+
T5760	10153-10154	-RRB-	denotes	)
T5761	10155-10165	JJ	denotes	transgenic
T5755	10166-10171	NN	denotes	mouse
T5762	10172-10178	NN	denotes	bottom
T5763	10178-10179	.	denotes	.
T5764	10179-10262	sentence	denotes	(j) CK6/ECFP (TG/+) transgenic mouse left, YC5/EYFP (TG/+) transgenic mouse right.
T5765	10180-10181	-LRB-	denotes	(
T5766	10181-10182	LS	denotes	j
T5768	10182-10183	-RRB-	denotes	)
T5769	10184-10187	NN	denotes	CK6
T5771	10187-10188	HYPH	denotes	/
T5770	10188-10192	NN	denotes	ECFP
T5772	10193-10194	-LRB-	denotes	(
T5773	10194-10196	NN	denotes	TG
T5774	10196-10197	HYPH	denotes	/
T5775	10197-10198	SYM	denotes	+
T5776	10198-10199	-RRB-	denotes	)
T5777	10200-10210	JJ	denotes	transgenic
T5767	10211-10216	NN	denotes	mouse
T5778	10217-10221	NN	denotes	left
T5779	10221-10223	,	denotes	,
T5780	10223-10226	NN	denotes	YC5
T5782	10226-10227	HYPH	denotes	/
T5781	10227-10231	NN	denotes	EYFP
T5784	10232-10233	-LRB-	denotes	(
T5785	10233-10235	NN	denotes	TG
T5786	10235-10236	HYPH	denotes	/
T5787	10236-10237	SYM	denotes	+
T5788	10237-10238	-RRB-	denotes	)
T5789	10239-10249	JJ	denotes	transgenic
T5783	10250-10255	NN	denotes	mouse
T5790	10256-10261	NN	denotes	right
T5791	10261-10262	.	denotes	.
T5792	10262-10330	sentence	denotes	(k) higher maginification view of the tails of the mice in i and j.
T5793	10263-10264	-LRB-	denotes	(
T5794	10264-10265	LS	denotes	k
T5796	10265-10266	-RRB-	denotes	)
T5797	10267-10273	JJR	denotes	higher
T5798	10274-10288	NN	denotes	maginification
T5795	10289-10293	NN	denotes	view
T5799	10294-10296	IN	denotes	of
T5800	10297-10300	DT	denotes	the
T5801	10301-10306	NNS	denotes	tails
T5802	10307-10309	IN	denotes	of
T5803	10310-10313	DT	denotes	the
T5804	10314-10318	NNS	denotes	mice
T5805	10319-10321	IN	denotes	in
T5806	10322-10323	NN	denotes	i
T5807	10324-10327	CC	denotes	and
T5808	10328-10329	NN	denotes	j
T5809	10329-10330	.	denotes	.
T2119	10331-10338	JJ	denotes	Zygotic
T2118	10331-10476	sentence	denotes	Zygotic expression of the FP transgenes is clearly evident by the blastocyst stage (Fig. 2a,2b,2c,2d) and remains widespread thereafter (Fig. 2).
T2120	10339-10349	NN	denotes	expression
T2122	10350-10352	IN	denotes	of
T2123	10353-10356	DT	denotes	the
T2125	10357-10359	NN	denotes	FP
T2124	10360-10370	NNS	denotes	transgenes
T2121	10371-10373	VBZ	denotes	is
T2126	10374-10381	RB	denotes	clearly
T2127	10382-10389	JJ	denotes	evident
T2128	10390-10392	IN	denotes	by
T2129	10393-10396	DT	denotes	the
T2131	10397-10407	NN	denotes	blastocyst
T2130	10408-10413	NN	denotes	stage
T2132	10414-10415	-LRB-	denotes	(
T2134	10415-10419	NN	denotes	Fig.
T2135	10420-10422	NN	denotes	2a
T2136	10422-10423	,	denotes	,
T2137	10423-10425	NN	denotes	2b
T2138	10425-10426	,	denotes	,
T2139	10426-10428	NN	denotes	2c
T2140	10428-10429	,	denotes	,
T2133	10429-10431	NN	denotes	2d
T2141	10431-10432	-RRB-	denotes	)
T2142	10433-10436	CC	denotes	and
T2143	10437-10444	VBZ	denotes	remains
T2144	10445-10455	JJ	denotes	widespread
T2145	10456-10466	RB	denotes	thereafter
T2146	10467-10468	-LRB-	denotes	(
T2147	10468-10472	NN	denotes	Fig.
T2148	10473-10474	CD	denotes	2
T2149	10474-10475	-RRB-	denotes	)
T2150	10475-10476	.	denotes	.
T2151	10476-10706	sentence	denotes	Figure 2 demonstrates that ECFP+ embryos can easily be distinguished from EYFP+ embryos and from non-transgenic non-fluorescent embryos from preimplantation stages (Fig. 2a,2b,2c,2d) to postimplantation stages (Fig. 2e,2f,2g,2h).
T2152	10477-10483	NN	denotes	Figure
T2154	10484-10485	CD	denotes	2
T2153	10486-10498	VBZ	denotes	demonstrates
T2155	10499-10503	IN	denotes	that
T2157	10504-10509	JJ	denotes	ECFP+
T2158	10510-10517	NNS	denotes	embryos
T2159	10518-10521	MD	denotes	can
T2160	10522-10528	RB	denotes	easily
T2161	10529-10531	VB	denotes	be
T2156	10532-10545	VBN	denotes	distinguished
T2162	10546-10550	IN	denotes	from
T2163	10551-10556	JJ	denotes	EYFP+
T2164	10557-10564	NNS	denotes	embryos
T2165	10565-10568	CC	denotes	and
T2166	10569-10573	IN	denotes	from
T2167	10574-10588	JJ	denotes	non-transgenic
T2169	10589-10604	JJ	denotes	non-fluorescent
T2168	10605-10612	NNS	denotes	embryos
T2170	10613-10617	IN	denotes	from
T2171	10618-10633	NN	denotes	preimplantation
T2172	10634-10640	NNS	denotes	stages
T2173	10641-10642	-LRB-	denotes	(
T2175	10642-10646	NN	denotes	Fig.
T2176	10647-10649	NN	denotes	2a
T2177	10649-10650	,	denotes	,
T2178	10650-10652	NN	denotes	2b
T2179	10652-10653	,	denotes	,
T2180	10653-10655	NN	denotes	2c
T2181	10655-10656	,	denotes	,
T2174	10656-10658	NN	denotes	2d
T2182	10658-10659	-RRB-	denotes	)
T2183	10660-10662	IN	denotes	to
T2184	10663-10679	NN	denotes	postimplantation
T2185	10680-10686	NNS	denotes	stages
T2186	10687-10688	-LRB-	denotes	(
T2188	10688-10692	NN	denotes	Fig.
T2189	10693-10695	NN	denotes	2e
T2190	10695-10696	,	denotes	,
T2191	10696-10698	NN	denotes	2f
T2192	10698-10699	,	denotes	,
T2193	10699-10701	NN	denotes	2g
T2194	10701-10702	,	denotes	,
T2187	10702-10704	NN	denotes	2h
T2195	10704-10705	-RRB-	denotes	)
T2196	10705-10706	.	denotes	.
T2197	10706-10821	sentence	denotes	Additionally adult mice can also be discriminated on the basis of the color of their fluorescence (Fig. 2i,2j,2k).
T2198	10707-10719	RB	denotes	Additionally
T2200	10720-10725	JJ	denotes	adult
T2201	10726-10730	NNS	denotes	mice
T2202	10731-10734	MD	denotes	can
T2203	10735-10739	RB	denotes	also
T2204	10740-10742	VB	denotes	be
T2199	10743-10756	VBN	denotes	discriminated
T2205	10757-10759	IN	denotes	on
T2206	10760-10763	DT	denotes	the
T2207	10764-10769	NN	denotes	basis
T2208	10770-10772	IN	denotes	of
T2209	10773-10776	DT	denotes	the
T2210	10777-10782	NN	denotes	color
T2211	10783-10785	IN	denotes	of
T2212	10786-10791	PRP$	denotes	their
T2213	10792-10804	NN	denotes	fluorescence
T2214	10805-10806	-LRB-	denotes	(
T2216	10806-10810	NN	denotes	Fig.
T2217	10811-10813	NN	denotes	2i
T2218	10813-10814	,	denotes	,
T2219	10814-10816	NN	denotes	2j
T2220	10816-10817	,	denotes	,
T2215	10817-10819	NN	denotes	2k
T2221	10819-10820	-RRB-	denotes	)
T2222	10820-10821	.	denotes	.
T2223	10821-11113	sentence	denotes	Even though our RFP transgenic ES cell lines failed to maintain strong uniform expression with in vitro passage, we selected three lines exhibiting the most robust expression for making chimeras, in order to ascertain whether we could achieve, or possibly recover, RFP expression in animals.
T2224	10822-10826	RB	denotes	Even
T2226	10827-10833	IN	denotes	though
T2227	10834-10837	PRP$	denotes	our
T2229	10838-10841	NN	denotes	RFP
T2230	10842-10852	JJ	denotes	transgenic
T2231	10853-10855	NN	denotes	ES
T2232	10856-10860	NN	denotes	cell
T2228	10861-10866	NNS	denotes	lines
T2225	10867-10873	VBD	denotes	failed
T2234	10874-10876	TO	denotes	to
T2235	10877-10885	VB	denotes	maintain
T2236	10886-10892	JJ	denotes	strong
T2238	10893-10900	JJ	denotes	uniform
T2237	10901-10911	NN	denotes	expression
T2239	10912-10916	IN	denotes	with
T2240	10917-10919	FW	denotes	in
T2241	10920-10925	FW	denotes	vitro
T2242	10926-10933	NN	denotes	passage
T2243	10933-10935	,	denotes	,
T2244	10935-10937	PRP	denotes	we
T2233	10938-10946	VBD	denotes	selected
T2245	10947-10952	CD	denotes	three
T2246	10953-10958	NNS	denotes	lines
T2247	10959-10969	VBG	denotes	exhibiting
T2248	10970-10973	DT	denotes	the
T2250	10974-10978	RBS	denotes	most
T2251	10979-10985	JJ	denotes	robust
T2249	10986-10996	NN	denotes	expression
T2252	10997-11000	IN	denotes	for
T2253	11001-11007	VBG	denotes	making
T2254	11008-11016	NNS	denotes	chimeras
T2255	11016-11018	,	denotes	,
T2256	11018-11020	IN	denotes	in
T2257	11021-11026	NN	denotes	order
T2258	11027-11029	TO	denotes	to
T2259	11030-11039	VB	denotes	ascertain
T2260	11040-11047	IN	denotes	whether
T2262	11048-11050	PRP	denotes	we
T2263	11051-11056	MD	denotes	could
T2261	11057-11064	VB	denotes	achieve
T2264	11064-11066	,	denotes	,
T2265	11066-11068	CC	denotes	or
T2266	11069-11077	RB	denotes	possibly
T2267	11078-11085	VB	denotes	recover
T2268	11085-11087	,	denotes	,
T2269	11087-11090	NN	denotes	RFP
T2270	11091-11101	NN	denotes	expression
T2271	11102-11104	IN	denotes	in
T2272	11105-11112	NNS	denotes	animals
T2273	11112-11113	.	denotes	.
T2274	11113-11193	sentence	denotes	Cells were flow sorted prior to aggregation so as to enrich for RFP expression.
T2275	11114-11119	NNS	denotes	Cells
T2277	11120-11124	VBD	denotes	were
T2278	11125-11129	NN	denotes	flow
T2276	11130-11136	VBN	denotes	sorted
T2279	11137-11142	RB	denotes	prior
T2280	11143-11145	IN	denotes	to
T2281	11146-11157	NN	denotes	aggregation
T2282	11158-11160	RB	denotes	so
T2284	11161-11163	IN	denotes	as
T2285	11164-11166	TO	denotes	to
T2283	11167-11173	VB	denotes	enrich
T2286	11174-11177	IN	denotes	for
T2287	11178-11181	NN	denotes	RFP
T2288	11182-11192	NN	denotes	expression
T2289	11192-11193	.	denotes	.
T2290	11193-11378	sentence	denotes	Even though they had lost their characteristic ES cell morphology all three RFP+ ES cell lines went gemline as detected by coat color and PCR for the DsRed1 transgene (data not shown).
T2291	11194-11198	RB	denotes	Even
T2293	11199-11205	IN	denotes	though
T2294	11206-11210	PRP	denotes	they
T2295	11211-11214	VBD	denotes	had
T2292	11215-11219	VBN	denotes	lost
T2297	11220-11225	PRP$	denotes	their
T2299	11226-11240	JJ	denotes	characteristic
T2300	11241-11243	NN	denotes	ES
T2301	11244-11248	NN	denotes	cell
T2298	11249-11259	NN	denotes	morphology
T2302	11260-11263	DT	denotes	all
T2304	11264-11269	CD	denotes	three
T2305	11270-11274	JJ	denotes	RFP+
T2306	11275-11277	NN	denotes	ES
T2307	11278-11282	NN	denotes	cell
T2303	11283-11288	NNS	denotes	lines
T2296	11289-11293	VBD	denotes	went
T2308	11294-11301	RB	denotes	gemline
T2309	11302-11304	IN	denotes	as
T2310	11305-11313	VBN	denotes	detected
T2311	11314-11316	IN	denotes	by
T2312	11317-11321	NN	denotes	coat
T2313	11322-11327	NN	denotes	color
T2314	11328-11331	CC	denotes	and
T2315	11332-11335	NN	denotes	PCR
T2316	11336-11339	IN	denotes	for
T2317	11340-11343	DT	denotes	the
T2319	11344-11350	NN	denotes	DsRed1
T2318	11351-11360	NN	denotes	transgene
T2320	11361-11362	-LRB-	denotes	(
T2322	11362-11366	NNS	denotes	data
T2323	11367-11370	RB	denotes	not
T2321	11371-11376	VBN	denotes	shown
T2324	11376-11377	-RRB-	denotes	)
T2325	11377-11378	.	denotes	.
T2326	11378-11499	sentence	denotes	However expression of the RFP reporter could not be detected in any organ of adult mice nor in embryos (data not shown).
T2327	11379-11386	RB	denotes	However
T2329	11387-11397	NN	denotes	expression
T2330	11398-11400	IN	denotes	of
T2331	11401-11404	DT	denotes	the
T2333	11405-11408	NN	denotes	RFP
T2332	11409-11417	NN	denotes	reporter
T2334	11418-11423	MD	denotes	could
T2335	11424-11427	RB	denotes	not
T2336	11428-11430	VB	denotes	be
T2328	11431-11439	VBN	denotes	detected
T2337	11440-11442	IN	denotes	in
T2338	11443-11446	DT	denotes	any
T2339	11447-11452	NN	denotes	organ
T2340	11453-11455	IN	denotes	of
T2341	11456-11461	JJ	denotes	adult
T2342	11462-11466	NNS	denotes	mice
T2343	11467-11470	CC	denotes	nor
T2344	11471-11473	IN	denotes	in
T2345	11474-11481	NNS	denotes	embryos
T2346	11482-11483	-LRB-	denotes	(
T2348	11483-11487	NNS	denotes	data
T2349	11488-11491	RB	denotes	not
T2347	11492-11497	VBN	denotes	shown
T2350	11497-11498	-RRB-	denotes	)
T2351	11498-11499	.	denotes	.
T2352	11499-11639	sentence	denotes	This suggests that the DsRed1 RFP reporter was unable to recapitulate the expression profile of the promoter/enhancer combination employed.
T2353	11500-11504	DT	denotes	This
T2354	11505-11513	VBZ	denotes	suggests
T2355	11514-11518	IN	denotes	that
T2357	11519-11522	DT	denotes	the
T2359	11523-11529	NN	denotes	DsRed1
T2360	11530-11533	NN	denotes	RFP
T2358	11534-11542	NN	denotes	reporter
T2356	11543-11546	VBD	denotes	was
T2361	11547-11553	JJ	denotes	unable
T2362	11554-11556	TO	denotes	to
T2363	11557-11569	VB	denotes	recapitulate
T2364	11570-11573	DT	denotes	the
T2366	11574-11584	NN	denotes	expression
T2365	11585-11592	NN	denotes	profile
T2367	11593-11595	IN	denotes	of
T2368	11596-11599	DT	denotes	the
T2370	11600-11608	NN	denotes	promoter
T2372	11608-11609	HYPH	denotes	/
T2371	11609-11617	NN	denotes	enhancer
T2369	11618-11629	NN	denotes	combination
T2373	11630-11638	VBN	denotes	employed
T2374	11638-11639	.	denotes	.
T2375	11639-11749	sentence	denotes	Therefore it appears that GFP variant reporters are more versatile with respect to their application in mice.
T2376	11640-11649	RB	denotes	Therefore
T2378	11650-11652	PRP	denotes	it
T2377	11653-11660	VBZ	denotes	appears
T2379	11661-11665	IN	denotes	that
T2381	11666-11669	NN	denotes	GFP
T2383	11670-11677	JJ	denotes	variant
T2382	11678-11687	NNS	denotes	reporters
T2380	11688-11691	VBP	denotes	are
T2384	11692-11696	RBR	denotes	more
T2385	11697-11706	JJ	denotes	versatile
T2386	11707-11711	IN	denotes	with
T2387	11712-11719	NN	denotes	respect
T2388	11720-11722	IN	denotes	to
T2389	11723-11728	PRP$	denotes	their
T2390	11729-11740	NN	denotes	application
T2391	11741-11743	IN	denotes	in
T2392	11744-11748	NNS	denotes	mice
T2393	11748-11749	.	denotes	.
T2394	11749-11889	sentence	denotes	We produced double FP-tagged chimeras in order to assess whether the ECFP and EYFP reporters could be co-visualized within the same animal.
T2395	11750-11752	PRP	denotes	We
T2396	11753-11761	VBD	denotes	produced
T2397	11762-11768	JJ	denotes	double
T2399	11769-11771	NN	denotes	FP
T2401	11771-11772	HYPH	denotes	-
T2400	11772-11778	VBN	denotes	tagged
T2398	11779-11787	NNS	denotes	chimeras
T2402	11788-11790	IN	denotes	in
T2403	11791-11796	NN	denotes	order
T2404	11797-11799	TO	denotes	to
T2405	11800-11806	VB	denotes	assess
T2406	11807-11814	IN	denotes	whether
T2408	11815-11818	DT	denotes	the
T2410	11819-11823	NN	denotes	ECFP
T2411	11824-11827	CC	denotes	and
T2412	11828-11832	NN	denotes	EYFP
T2409	11833-11842	NNS	denotes	reporters
T2413	11843-11848	MD	denotes	could
T2414	11849-11851	VB	denotes	be
T2407	11852-11865	VBN	denotes	co-visualized
T2415	11866-11872	IN	denotes	within
T2416	11873-11876	DT	denotes	the
T2418	11877-11881	JJ	denotes	same
T2417	11882-11888	NN	denotes	animal
T2419	11888-11889	.	denotes	.
T2420	11889-12038	sentence	denotes	The first series of chimeras were generated through the aggregation of tetraploid embryos with FP+ ES cells (or embryos) of the complementary color.
T2421	11890-11893	DT	denotes	The
T2423	11894-11899	JJ	denotes	first
T2422	11900-11906	NN	denotes	series
T2425	11907-11909	IN	denotes	of
T2426	11910-11918	NNS	denotes	chimeras
T2427	11919-11923	VBD	denotes	were
T2424	11924-11933	VBN	denotes	generated
T2428	11934-11941	IN	denotes	through
T2429	11942-11945	DT	denotes	the
T2430	11946-11957	NN	denotes	aggregation
T2431	11958-11960	IN	denotes	of
T2432	11961-11971	JJ	denotes	tetraploid
T2433	11972-11979	NNS	denotes	embryos
T2434	11980-11984	IN	denotes	with
T2435	11985-11988	JJ	denotes	FP+
T2437	11989-11991	NN	denotes	ES
T2436	11992-11997	NNS	denotes	cells
T2438	11998-11999	-LRB-	denotes	(
T2439	11999-12001	CC	denotes	or
T2440	12002-12009	NNS	denotes	embryos
T2441	12009-12010	-RRB-	denotes	)
T2442	12011-12013	IN	denotes	of
T2443	12014-12017	DT	denotes	the
T2445	12018-12031	JJ	denotes	complementary
T2444	12032-12037	NN	denotes	color
T2446	12037-12038	.	denotes	.
T2447	12038-12079	sentence	denotes	The procedure is schematized in Fig. 3a.
T2448	12039-12042	DT	denotes	The
T2449	12043-12052	NN	denotes	procedure
T2451	12053-12055	VBZ	denotes	is
T2450	12056-12067	VBN	denotes	schematized
T2452	12068-12070	IN	denotes	in
T2453	12071-12075	NN	denotes	Fig.
T2454	12076-12078	NN	denotes	3a
T2455	12078-12079	.	denotes	.
T2456	12079-12319	sentence	denotes	It is evident that the expression of the two FPs can easily be discerned in these double transgenic embryos where the diploid, embryo-proper compartment is ECFP+ and the tetraploid extraembryonic compartment is EYFP+ (Fig. 3b,3c,3d,3e,3f).
T2457	12080-12082	PRP	denotes	It
T2458	12083-12085	VBZ	denotes	is
T2459	12086-12093	JJ	denotes	evident
T2460	12094-12098	IN	denotes	that
T2462	12099-12102	DT	denotes	the
T2463	12103-12113	NN	denotes	expression
T2464	12114-12116	IN	denotes	of
T2465	12117-12120	DT	denotes	the
T2467	12121-12124	CD	denotes	two
T2466	12125-12128	NNS	denotes	FPs
T2468	12129-12132	MD	denotes	can
T2469	12133-12139	RB	denotes	easily
T2470	12140-12142	VB	denotes	be
T2461	12143-12152	VBN	denotes	discerned
T2471	12153-12155	IN	denotes	in
T2472	12156-12161	DT	denotes	these
T2474	12162-12168	JJ	denotes	double
T2475	12169-12179	JJ	denotes	transgenic
T2473	12180-12187	NNS	denotes	embryos
T2476	12188-12193	WRB	denotes	where
T2478	12194-12197	DT	denotes	the
T2480	12198-12205	JJ	denotes	diploid
T2481	12205-12207	,	denotes	,
T2482	12207-12213	NN	denotes	embryo
T2484	12213-12214	HYPH	denotes	-
T2483	12214-12220	JJ	denotes	proper
T2479	12221-12232	NN	denotes	compartment
T2477	12233-12235	VBZ	denotes	is
T2485	12236-12241	JJ	denotes	ECFP+
T2486	12242-12245	CC	denotes	and
T2487	12246-12249	DT	denotes	the
T2489	12250-12260	JJ	denotes	tetraploid
T2490	12261-12275	JJ	denotes	extraembryonic
T2488	12276-12287	NN	denotes	compartment
T2491	12288-12290	VBZ	denotes	is
T2492	12291-12296	JJ	denotes	EYFP+
T2493	12297-12298	-LRB-	denotes	(
T2495	12298-12302	NNP	denotes	Fig.
T2496	12303-12305	NN	denotes	3b
T2497	12305-12306	,	denotes	,
T2498	12306-12308	NN	denotes	3c
T2499	12308-12309	,	denotes	,
T2500	12309-12311	NN	denotes	3d
T2501	12311-12312	,	denotes	,
T2502	12312-12314	NN	denotes	3e
T2503	12314-12315	,	denotes	,
T2494	12315-12317	NN	denotes	3f
T2504	12317-12318	-RRB-	denotes	)
T2505	12318-12319	.	denotes	.
T2506	12319-12445	sentence	denotes	Secondly we investigated reporter co-visualization in balanced adult chimeras comprised of both ECFP+ and EYFP+ compartments.
T2507	12320-12328	RB	denotes	Secondly
T2509	12329-12331	PRP	denotes	we
T2508	12332-12344	VBD	denotes	investigated
T2510	12345-12353	NN	denotes	reporter
T2511	12354-12370	NN	denotes	co-visualization
T2512	12371-12373	IN	denotes	in
T2513	12374-12382	JJ	denotes	balanced
T2515	12383-12388	JJ	denotes	adult
T2514	12389-12397	NNS	denotes	chimeras
T2516	12398-12407	VBN	denotes	comprised
T2517	12408-12410	IN	denotes	of
T2518	12411-12415	CC	denotes	both
T2520	12416-12421	JJ	denotes	ECFP+
T2521	12422-12425	CC	denotes	and
T2522	12426-12431	JJ	denotes	EYFP+
T2519	12432-12444	NNS	denotes	compartments
T2523	12444-12445	.	denotes	.
T2524	12445-12572	sentence	denotes	These were generated through the aggregation of diploid embryos with diploid embryos (or ES cells) of the complementary color.
T2525	12446-12451	DT	denotes	These
T2527	12452-12456	VBD	denotes	were
T2526	12457-12466	VBN	denotes	generated
T2528	12467-12474	IN	denotes	through
T2529	12475-12478	DT	denotes	the
T2530	12479-12490	NN	denotes	aggregation
T2531	12491-12493	IN	denotes	of
T2532	12494-12501	JJ	denotes	diploid
T2533	12502-12509	NNS	denotes	embryos
T2534	12510-12514	IN	denotes	with
T2535	12515-12522	JJ	denotes	diploid
T2536	12523-12530	NNS	denotes	embryos
T2537	12531-12532	-LRB-	denotes	(
T2538	12532-12534	CC	denotes	or
T2539	12535-12537	NN	denotes	ES
T2540	12538-12543	NNS	denotes	cells
T2541	12543-12544	-RRB-	denotes	)
T2542	12545-12547	IN	denotes	of
T2543	12548-12551	DT	denotes	the
T2545	12552-12565	JJ	denotes	complementary
T2544	12566-12571	NN	denotes	color
T2546	12571-12572	.	denotes	.
T2547	12572-12703	sentence	denotes	The aggregated components of the chimera from which each organ originated is schematized in the top right of each panel in Fig. 4.
T2548	12573-12576	DT	denotes	The
T2550	12577-12587	JJ	denotes	aggregated
T2549	12588-12598	NNS	denotes	components
T2552	12599-12601	IN	denotes	of
T2553	12602-12605	DT	denotes	the
T2554	12606-12613	NN	denotes	chimera
T2555	12614-12618	IN	denotes	from
T2557	12619-12624	WDT	denotes	which
T2558	12625-12629	DT	denotes	each
T2559	12630-12635	NN	denotes	organ
T2556	12636-12646	VBD	denotes	originated
T2560	12647-12649	VBZ	denotes	is
T2551	12650-12661	VBN	denotes	schematized
T2561	12662-12664	IN	denotes	in
T2562	12665-12668	DT	denotes	the
T2564	12669-12672	JJ	denotes	top
T2563	12673-12678	NN	denotes	right
T2565	12679-12681	IN	denotes	of
T2566	12682-12686	DT	denotes	each
T2567	12687-12692	NN	denotes	panel
T2568	12693-12695	IN	denotes	in
T2569	12696-12700	NNP	denotes	Fig.
T2570	12701-12702	CD	denotes	4
T2571	12702-12703	.	denotes	.
T2572	12703-12834	sentence	denotes	These chimeric adult organs demonstrate that the ECFP+ and EYFP+ cells comprising these chimeras can easily be discerned (Fig. 4).
T2573	12704-12709	DT	denotes	These
T2575	12710-12718	JJ	denotes	chimeric
T2576	12719-12724	JJ	denotes	adult
T2574	12725-12731	NNS	denotes	organs
T2577	12732-12743	VBP	denotes	demonstrate
T2578	12744-12748	IN	denotes	that
T2580	12749-12752	DT	denotes	the
T2582	12753-12758	JJ	denotes	ECFP+
T2583	12759-12762	CC	denotes	and
T2584	12763-12768	JJ	denotes	EYFP+
T2581	12769-12774	NNS	denotes	cells
T2585	12775-12785	VBG	denotes	comprising
T2586	12786-12791	DT	denotes	these
T2587	12792-12800	NNS	denotes	chimeras
T2588	12801-12804	MD	denotes	can
T2589	12805-12811	RB	denotes	easily
T2590	12812-12814	VB	denotes	be
T2579	12815-12824	VBN	denotes	discerned
T2591	12825-12826	-LRB-	denotes	(
T2592	12826-12830	NN	denotes	Fig.
T2593	12831-12832	CD	denotes	4
T2594	12832-12833	-RRB-	denotes	)
T2595	12833-12834	.	denotes	.
T2596	12834-12988	sentence	denotes	It can therefore be envisaged that two different FP colors could be used in the construction of chimeras so as to tag both mutant and wild type lineages.
T2597	12835-12837	PRP	denotes	It
T2599	12838-12841	MD	denotes	can
T2600	12842-12851	RB	denotes	therefore
T2601	12852-12854	VB	denotes	be
T2598	12855-12864	VBN	denotes	envisaged
T2602	12865-12869	IN	denotes	that
T2604	12870-12873	CD	denotes	two
T2606	12874-12883	JJ	denotes	different
T2607	12884-12886	NN	denotes	FP
T2605	12887-12893	NNS	denotes	colors
T2608	12894-12899	MD	denotes	could
T2609	12900-12902	VB	denotes	be
T2603	12903-12907	VBN	denotes	used
T2610	12908-12910	IN	denotes	in
T2611	12911-12914	DT	denotes	the
T2612	12915-12927	NN	denotes	construction
T2613	12928-12930	IN	denotes	of
T2614	12931-12939	NNS	denotes	chimeras
T2615	12940-12942	RB	denotes	so
T2617	12943-12945	IN	denotes	as
T2618	12946-12948	TO	denotes	to
T2616	12949-12952	VB	denotes	tag
T2619	12953-12957	CC	denotes	both
T2621	12958-12964	JJ	denotes	mutant
T2622	12965-12968	CC	denotes	and
T2623	12969-12973	JJ	denotes	wild
T2624	12974-12978	NN	denotes	type
T2620	12979-12987	NNS	denotes	lineages
T2625	12987-12988	.	denotes	.
T5891	12999-13003	JJ	denotes	Dual
T5893	13004-13016	JJ	denotes	non-invasive
T5894	13017-13025	NN	denotes	reporter
T5892	13026-13039	NN	denotes	visualization
T5895	13040-13042	IN	denotes	in
T5896	13043-13051	JJ	denotes	chimeric
T5897	13052-13059	NNS	denotes	embryos
T5898	13060-13069	VBN	denotes	generated
T5899	13070-13072	IN	denotes	by
T5900	13073-13083	JJ	denotes	tetraploid
T5902	13084-13085	SYM	denotes	<
T5903	13085-13086	HYPH	denotes	-
T5904	13086-13087	SYM	denotes	>
T5905	13088-13095	JJ	denotes	diploid
T5901	13096-13107	NN	denotes	aggregation
T5906	13107-13108	.	denotes	.
T5907	13108-13235	sentence	denotes	In all panels the tetraploid compartment is yellow/green fluorescent (EYFP+) and the diploid is blue/cyan fluorescent (ECFP+).
T5908	13109-13111	IN	denotes	In
T5910	13112-13115	DT	denotes	all
T5911	13116-13122	NNS	denotes	panels
T5912	13123-13126	DT	denotes	the
T5914	13127-13137	JJ	denotes	tetraploid
T5913	13138-13149	NN	denotes	compartment
T5909	13150-13152	VBZ	denotes	is
T5915	13153-13159	JJ	denotes	yellow
T5917	13159-13160	HYPH	denotes	/
T5916	13160-13165	JJ	denotes	green
T5918	13166-13177	JJ	denotes	fluorescent
T5920	13178-13179	-LRB-	denotes	(
T5919	13179-13184	JJ	denotes	EYFP+
T5921	13184-13185	-RRB-	denotes	)
T5922	13186-13189	CC	denotes	and
T5923	13190-13193	DT	denotes	the
T5924	13194-13201	NN	denotes	diploid
T5925	13202-13204	VBZ	denotes	is
T5926	13205-13209	JJ	denotes	blue
T5928	13209-13210	HYPH	denotes	/
T5927	13210-13214	JJ	denotes	cyan
T5929	13215-13226	JJ	denotes	fluorescent
T5931	13227-13228	-LRB-	denotes	(
T5930	13228-13233	JJ	denotes	ECFP+
T5932	13233-13234	-RRB-	denotes	)
T5933	13234-13235	.	denotes	.
T5934	13235-13293	sentence	denotes	(a) schematic representation of the tetraploid procedure.
T5935	13236-13237	-LRB-	denotes	(
T5936	13237-13238	LS	denotes	a
T5938	13238-13239	-RRB-	denotes	)
T5939	13240-13249	JJ	denotes	schematic
T5937	13250-13264	NN	denotes	representation
T5940	13265-13267	IN	denotes	of
T5941	13268-13271	DT	denotes	the
T5943	13272-13282	JJ	denotes	tetraploid
T5942	13283-13292	NN	denotes	procedure
T5944	13292-13293	.	denotes	.
T5945	13293-13448	sentence	denotes	Electrofusion of E1.5 embryos to render the blastomeres tetraploid, in vitro culture, and subsequent aggregation schemes using double-tagged compartments.
T5946	13294-13307	NN	denotes	Electrofusion
T5947	13308-13310	IN	denotes	of
T5948	13311-13315	NN	denotes	E1.5
T5949	13316-13323	NNS	denotes	embryos
T5950	13324-13326	TO	denotes	to
T5951	13327-13333	VB	denotes	render
T5952	13334-13337	DT	denotes	the
T5953	13338-13349	NNS	denotes	blastomeres
T5954	13350-13360	JJ	denotes	tetraploid
T5955	13360-13362	,	denotes	,
T5956	13362-13364	FW	denotes	in
T5957	13365-13370	FW	denotes	vitro
T5958	13371-13378	NN	denotes	culture
T5959	13378-13380	,	denotes	,
T5960	13380-13383	CC	denotes	and
T5961	13384-13394	JJ	denotes	subsequent
T5963	13395-13406	NN	denotes	aggregation
T5962	13407-13414	NNS	denotes	schemes
T5964	13415-13420	VBG	denotes	using
T5965	13421-13427	JJ	denotes	double
T5967	13427-13428	HYPH	denotes	-
T5966	13428-13434	JJ	denotes	tagged
T5968	13435-13447	NNS	denotes	compartments
T5969	13447-13448	.	denotes	.
T5970	13448-13530	sentence	denotes	Sandwich aggregations were made using either ECFP+ (diploid) morulae or ES cells.
T5971	13449-13457	JJ	denotes	Sandwich
T5972	13458-13470	NNS	denotes	aggregations
T5974	13471-13475	VBD	denotes	were
T5973	13476-13480	VBN	denotes	made
T5975	13481-13486	VBG	denotes	using
T5976	13487-13493	CC	denotes	either
T5978	13494-13499	JJ	denotes	ECFP+
T5979	13500-13501	-LRB-	denotes	(
T5980	13501-13508	JJ	denotes	diploid
T5981	13508-13509	-RRB-	denotes	)
T5977	13510-13517	NNS	denotes	morulae
T5982	13518-13520	CC	denotes	or
T5983	13521-13523	NN	denotes	ES
T5984	13524-13529	NNS	denotes	cells
T5985	13529-13530	.	denotes	.
T5986	13530-13689	sentence	denotes	(b-d) anterior view of an E7.5 double-tagged polarized chimera dissected free of its deciduum and therefore missing primary giant cells and parietal endoderm.
T5987	13531-13532	-LRB-	denotes	(
T5988	13532-13533	LS	denotes	b
T5990	13533-13534	SYM	denotes	-
T5991	13534-13535	LS	denotes	d
T5992	13535-13536	-RRB-	denotes	)
T5993	13537-13545	JJ	denotes	anterior
T5989	13546-13550	NN	denotes	view
T5994	13551-13553	IN	denotes	of
T5995	13554-13556	DT	denotes	an
T5997	13557-13561	NN	denotes	E7.5
T5998	13562-13568	JJ	denotes	double
T6000	13568-13569	HYPH	denotes	-
T5999	13569-13575	JJ	denotes	tagged
T6001	13576-13585	VBN	denotes	polarized
T5996	13586-13593	NN	denotes	chimera
T6002	13594-13603	VBN	denotes	dissected
T6003	13604-13608	JJ	denotes	free
T6004	13609-13611	IN	denotes	of
T6005	13612-13615	PRP$	denotes	its
T6006	13616-13624	NN	denotes	deciduum
T6007	13625-13628	CC	denotes	and
T6008	13629-13638	RB	denotes	therefore
T6009	13639-13646	VBG	denotes	missing
T6010	13647-13654	JJ	denotes	primary
T6012	13655-13660	JJ	denotes	giant
T6011	13661-13666	NNS	denotes	cells
T6013	13667-13670	CC	denotes	and
T6014	13671-13679	JJ	denotes	parietal
T6015	13680-13688	NN	denotes	endoderm
T6016	13688-13689	.	denotes	.
T6017	13689-13869	sentence	denotes	Here the epiblast (lower half of embryo) and its derivatives are ECFP+ whereas the extraembryonic ectoderm, the visceral endoderm and trophoblast are EYFP+ (upper half or embryo).
T6018	13690-13694	RB	denotes	Here
T6020	13695-13698	DT	denotes	the
T6021	13699-13707	NN	denotes	epiblast
T6022	13708-13709	-LRB-	denotes	(
T6024	13709-13714	JJR	denotes	lower
T6023	13715-13719	NN	denotes	half
T6025	13720-13722	IN	denotes	of
T6026	13723-13729	NN	denotes	embryo
T6027	13729-13730	-RRB-	denotes	)
T6028	13731-13734	CC	denotes	and
T6029	13735-13738	PRP$	denotes	its
T6030	13739-13750	NNS	denotes	derivatives
T6019	13751-13754	VBP	denotes	are
T6031	13755-13760	JJ	denotes	ECFP+
T6032	13761-13768	IN	denotes	whereas
T6034	13769-13772	DT	denotes	the
T6036	13773-13787	JJ	denotes	extraembryonic
T6035	13788-13796	NN	denotes	ectoderm
T6037	13796-13798	,	denotes	,
T6038	13798-13801	DT	denotes	the
T6040	13802-13810	JJ	denotes	visceral
T6039	13811-13819	NN	denotes	endoderm
T6041	13820-13823	CC	denotes	and
T6042	13824-13835	NN	denotes	trophoblast
T6033	13836-13839	VBP	denotes	are
T6043	13840-13845	JJ	denotes	EYFP+
T6044	13846-13847	-LRB-	denotes	(
T6046	13847-13852	JJ	denotes	upper
T6045	13853-13857	NN	denotes	half
T6047	13858-13860	CC	denotes	or
T6048	13861-13867	NN	denotes	embryo
T6049	13867-13868	-RRB-	denotes	)
T6050	13868-13869	.	denotes	.
T6051	13869-13920	sentence	denotes	(b) dark field image taken through an EYFP filter.
T6052	13870-13871	-LRB-	denotes	(
T6053	13871-13872	LS	denotes	b
T6055	13872-13873	-RRB-	denotes	)
T6056	13874-13878	JJ	denotes	dark
T6057	13879-13884	NN	denotes	field
T6054	13885-13890	NN	denotes	image
T6058	13891-13896	VBN	denotes	taken
T6059	13897-13904	IN	denotes	through
T6060	13905-13907	DT	denotes	an
T6062	13908-13912	NN	denotes	EYFP
T6061	13913-13919	NN	denotes	filter
T6063	13919-13920	.	denotes	.
T6064	13920-13971	sentence	denotes	(c) dark field image taken through an ECFP filter.
T6065	13921-13922	-LRB-	denotes	(
T6066	13922-13923	LS	denotes	c
T6068	13923-13924	-RRB-	denotes	)
T6069	13925-13929	VB	denotes	dark
T6070	13930-13935	NN	denotes	field
T6067	13936-13941	NN	denotes	image
T6071	13942-13947	VBN	denotes	taken
T6072	13948-13955	IN	denotes	through
T6073	13956-13958	DT	denotes	an
T6075	13959-13963	NN	denotes	ECFP
T6074	13964-13970	NN	denotes	filter
T6076	13970-13971	.	denotes	.
T6077	13971-14063	sentence	denotes	(d) dark field double exposure image acquired by consecutively using ECFP and EYFP filters.
T6078	13972-13973	-LRB-	denotes	(
T6079	13973-13974	LS	denotes	d
T6081	13974-13975	-RRB-	denotes	)
T6082	13976-13980	JJ	denotes	dark
T6083	13981-13986	NN	denotes	field
T6084	13987-13993	JJ	denotes	double
T6085	13994-14002	NN	denotes	exposure
T6080	14003-14008	NN	denotes	image
T6086	14009-14017	VBN	denotes	acquired
T6087	14018-14020	IN	denotes	by
T6088	14021-14034	RB	denotes	consecutively
T6089	14035-14040	VBG	denotes	using
T6090	14041-14045	NN	denotes	ECFP
T6092	14046-14049	CC	denotes	and
T6093	14050-14054	NN	denotes	EYFP
T6091	14055-14062	NNS	denotes	filters
T6094	14062-14063	.	denotes	.
T6095	14063-14216	sentence	denotes	(e) dark field double filter exposure of an E9 chimera dissected free of all extraembryonic membranes except for a small piece of yolk sac (lower left).
T6096	14064-14065	-LRB-	denotes	(
T6097	14065-14066	LS	denotes	e
T6099	14066-14067	-RRB-	denotes	)
T6100	14068-14072	JJ	denotes	dark
T6101	14073-14078	NN	denotes	field
T6102	14079-14085	JJ	denotes	double
T6103	14086-14092	NN	denotes	filter
T6098	14093-14101	NN	denotes	exposure
T6104	14102-14104	IN	denotes	of
T6105	14105-14107	DT	denotes	an
T6107	14108-14110	NN	denotes	E9
T6106	14111-14118	NN	denotes	chimera
T6108	14119-14128	VBN	denotes	dissected
T6109	14129-14133	JJ	denotes	free
T6110	14134-14136	IN	denotes	of
T6111	14137-14140	DT	denotes	all
T6113	14141-14155	JJ	denotes	extraembryonic
T6112	14156-14165	NNS	denotes	membranes
T6114	14166-14172	IN	denotes	except
T6115	14173-14176	IN	denotes	for
T6116	14177-14178	DT	denotes	a
T6118	14179-14184	JJ	denotes	small
T6117	14185-14190	NN	denotes	piece
T6119	14191-14193	IN	denotes	of
T6120	14194-14198	NN	denotes	yolk
T6121	14199-14202	NN	denotes	sac
T6122	14203-14204	-LRB-	denotes	(
T6124	14204-14209	JJR	denotes	lower
T6123	14210-14214	NN	denotes	left
T6125	14214-14215	-RRB-	denotes	)
T6126	14215-14216	.	denotes	.
T6127	14216-14328	sentence	denotes	The embryo itself is ECFP+ whereas the yolk sac both ECFP+ and EYFP+ in the mesoderm and endoderm respectively.
T6128	14217-14220	DT	denotes	The
T6129	14221-14227	NN	denotes	embryo
T6131	14228-14234	PRP	denotes	itself
T6130	14235-14237	VBZ	denotes	is
T6132	14238-14243	JJ	denotes	ECFP+
T6133	14244-14251	IN	denotes	whereas
T6135	14252-14255	DT	denotes	the
T6137	14256-14260	NN	denotes	yolk
T6136	14261-14264	NN	denotes	sac
T6138	14265-14269	CC	denotes	both
T6134	14270-14275	NN	denotes	ECFP+
T6139	14276-14279	CC	denotes	and
T6140	14280-14285	NN	denotes	EYFP+
T6141	14286-14288	IN	denotes	in
T6142	14289-14292	DT	denotes	the
T6143	14293-14301	NN	denotes	mesoderm
T6144	14302-14305	CC	denotes	and
T6145	14306-14314	NN	denotes	endoderm
T6146	14315-14327	RB	denotes	respectively
T6147	14327-14328	.	denotes	.
T6148	14328-14426	sentence	denotes	A small number of EYFP+ cells are also observed in the ventral midline of the embryo (arrowhead).
T6149	14329-14330	DT	denotes	A
T6151	14331-14336	JJ	denotes	small
T6150	14337-14343	NN	denotes	number
T6153	14344-14346	IN	denotes	of
T6154	14347-14352	JJ	denotes	EYFP+
T6155	14353-14358	NNS	denotes	cells
T6156	14359-14362	VBP	denotes	are
T6157	14363-14367	RB	denotes	also
T6152	14368-14376	VBN	denotes	observed
T6158	14377-14379	IN	denotes	in
T6159	14380-14383	DT	denotes	the
T6161	14384-14391	JJ	denotes	ventral
T6160	14392-14399	NN	denotes	midline
T6162	14400-14402	IN	denotes	of
T6163	14403-14406	DT	denotes	the
T6164	14407-14413	NN	denotes	embryo
T6165	14414-14415	-LRB-	denotes	(
T6166	14415-14424	NN	denotes	arrowhead
T6167	14424-14425	-RRB-	denotes	)
T6168	14425-14426	.	denotes	.
T6169	14426-14640	sentence	denotes	This observation has been noted previously in tetraploid chimeras, and is presumed to represent a small population of visceral endoderm cells that fail to be displaced by the definitive endoderm at earlier stages.
T6170	14427-14431	DT	denotes	This
T6171	14432-14443	NN	denotes	observation
T6173	14444-14447	VBZ	denotes	has
T6174	14448-14452	VBN	denotes	been
T6172	14453-14458	VBN	denotes	noted
T6175	14459-14469	RB	denotes	previously
T6176	14470-14472	IN	denotes	in
T6177	14473-14483	JJ	denotes	tetraploid
T6178	14484-14492	NNS	denotes	chimeras
T6179	14492-14494	,	denotes	,
T6180	14494-14497	CC	denotes	and
T6181	14498-14500	VBZ	denotes	is
T6182	14501-14509	VBN	denotes	presumed
T6183	14510-14512	TO	denotes	to
T6184	14513-14522	VB	denotes	represent
T6185	14523-14524	DT	denotes	a
T6187	14525-14530	JJ	denotes	small
T6186	14531-14541	NN	denotes	population
T6188	14542-14544	IN	denotes	of
T6189	14545-14553	JJ	denotes	visceral
T6191	14554-14562	NN	denotes	endoderm
T6190	14563-14568	NNS	denotes	cells
T6192	14569-14573	WDT	denotes	that
T6193	14574-14578	VBP	denotes	fail
T6194	14579-14581	TO	denotes	to
T6196	14582-14584	VB	denotes	be
T6195	14585-14594	VBN	denotes	displaced
T6197	14595-14597	IN	denotes	by
T6198	14598-14601	DT	denotes	the
T6200	14602-14612	JJ	denotes	definitive
T6199	14613-14621	NN	denotes	endoderm
T6201	14622-14624	IN	denotes	at
T6202	14625-14632	JJR	denotes	earlier
T6203	14633-14639	NNS	denotes	stages
T6204	14639-14640	.	denotes	.
T6205	14640-14697	sentence	denotes	(f) dark field double filter exposure of an E10 chimera.
T6206	14641-14642	-LRB-	denotes	(
T6207	14642-14643	LS	denotes	f
T6209	14643-14644	-RRB-	denotes	)
T6210	14645-14649	JJ	denotes	dark
T6211	14650-14655	NN	denotes	field
T6212	14656-14662	JJ	denotes	double
T6213	14663-14669	NN	denotes	filter
T6208	14670-14678	NN	denotes	exposure
T6214	14679-14681	IN	denotes	of
T6215	14682-14684	DT	denotes	an
T6217	14685-14688	NN	denotes	E10
T6216	14689-14696	NN	denotes	chimera
T6218	14696-14697	.	denotes	.
T6219	14697-14802	sentence	denotes	The embryo is exclusively ECFP+ whereas its placenta (to the left of the embryo) is predominantly EYFP+.
T6220	14698-14701	DT	denotes	The
T6221	14702-14708	NN	denotes	embryo
T6222	14709-14711	VBZ	denotes	is
T6223	14712-14723	RB	denotes	exclusively
T6224	14724-14729	JJ	denotes	ECFP+
T6225	14730-14737	IN	denotes	whereas
T6227	14738-14741	PRP$	denotes	its
T6228	14742-14750	NN	denotes	placenta
T6229	14751-14752	-LRB-	denotes	(
T6230	14752-14754	IN	denotes	to
T6231	14755-14758	DT	denotes	the
T6232	14759-14763	NN	denotes	left
T6233	14764-14766	IN	denotes	of
T6234	14767-14770	DT	denotes	the
T6235	14771-14777	NN	denotes	embryo
T6236	14777-14778	-RRB-	denotes	)
T6226	14779-14781	VBZ	denotes	is
T6237	14782-14795	RB	denotes	predominantly
T6238	14796-14801	JJ	denotes	EYFP+
T6239	14801-14802	.	denotes	.
T6240	14802-14891	sentence	denotes	The labyrinthine trophoblast layer of the placenta comprises both ECFP+ and EYFP+ cells.
T6241	14803-14806	DT	denotes	The
T6243	14807-14819	NN	denotes	labyrinthine
T6244	14820-14831	NN	denotes	trophoblast
T6242	14832-14837	NN	denotes	layer
T6246	14838-14840	IN	denotes	of
T6247	14841-14844	DT	denotes	the
T6248	14845-14853	NN	denotes	placenta
T6245	14854-14863	VBZ	denotes	comprises
T6249	14864-14868	CC	denotes	both
T6250	14869-14874	JJ	denotes	ECFP+
T6252	14875-14878	CC	denotes	and
T6253	14879-14884	JJ	denotes	EYFP+
T6251	14885-14890	NNS	denotes	cells
T6254	14890-14891	.	denotes	.
T6343	14902-14906	JJ	denotes	Dual
T6345	14907-14919	JJ	denotes	non-invasive
T6346	14920-14928	NN	denotes	reporter
T6344	14929-14942	NN	denotes	visualization
T6347	14943-14945	IN	denotes	in
T6348	14946-14949	DT	denotes	the
T6349	14950-14956	NNS	denotes	organs
T6350	14957-14959	IN	denotes	of
T6351	14960-14965	JJ	denotes	adult
T6352	14966-14974	NNS	denotes	chimeras
T6353	14974-14975	.	denotes	.
T6354	14975-15109	sentence	denotes	All images depict dark field double exposures acquired by consecutively using ECFP and EYFP filters under dark field epifluorescence.
T6355	14976-14979	DT	denotes	All
T6356	14980-14986	NNS	denotes	images
T6357	14987-14993	VBP	denotes	depict
T6358	14994-14998	JJ	denotes	dark
T6359	14999-15004	NN	denotes	field
T6361	15005-15011	JJ	denotes	double
T6360	15012-15021	NNS	denotes	exposures
T6362	15022-15030	VBN	denotes	acquired
T6363	15031-15033	IN	denotes	by
T6364	15034-15047	RB	denotes	consecutively
T6365	15048-15053	VBG	denotes	using
T6366	15054-15058	NN	denotes	ECFP
T6368	15059-15062	CC	denotes	and
T6369	15063-15067	NN	denotes	EYFP
T6367	15068-15075	NNS	denotes	filters
T6370	15076-15081	IN	denotes	under
T6371	15082-15086	JJ	denotes	dark
T6372	15087-15092	NN	denotes	field
T6373	15093-15108	NN	denotes	epifluorescence
T6374	15108-15109	.	denotes	.
T6375	15109-15179	sentence	denotes	Details of aggregates are schematized at the top right of each panel.
T6376	15110-15117	NNS	denotes	Details
T6378	15118-15120	IN	denotes	of
T6379	15121-15131	NNS	denotes	aggregates
T6380	15132-15135	VBP	denotes	are
T6377	15136-15147	VBN	denotes	schematized
T6381	15148-15150	IN	denotes	at
T6382	15151-15154	DT	denotes	the
T6384	15155-15158	JJ	denotes	top
T6383	15159-15164	NN	denotes	right
T6385	15165-15167	IN	denotes	of
T6386	15168-15172	DT	denotes	each
T6387	15173-15178	NN	denotes	panel
T6388	15178-15179	.	denotes	.
T6389	15179-15258	sentence	denotes	(a-c) heart and pancreas from double-tagged double-compartment adult chimeras.
T6390	15180-15181	-LRB-	denotes	(
T6391	15181-15182	LS	denotes	a
T6393	15182-15183	SYM	denotes	-
T6394	15183-15184	LS	denotes	c
T6395	15184-15185	-RRB-	denotes	)
T6392	15186-15191	NN	denotes	heart
T6396	15192-15195	CC	denotes	and
T6397	15196-15204	NN	denotes	pancreas
T6398	15205-15209	IN	denotes	from
T6399	15210-15216	JJ	denotes	double
T6401	15216-15217	HYPH	denotes	-
T6400	15217-15223	JJ	denotes	tagged
T6403	15224-15230	JJ	denotes	double
T6405	15230-15231	HYPH	denotes	-
T6404	15231-15242	NN	denotes	compartment
T6406	15243-15248	JJ	denotes	adult
T6402	15249-15257	NNS	denotes	chimeras
T6407	15257-15258	.	denotes	.
T6408	15258-15301	sentence	denotes	(a) view of the surface of an adult heart.
T6409	15259-15260	-LRB-	denotes	(
T6410	15260-15261	LS	denotes	a
T6412	15261-15262	-RRB-	denotes	)
T6411	15263-15267	NN	denotes	view
T6413	15268-15270	IN	denotes	of
T6414	15271-15274	DT	denotes	the
T6415	15275-15282	NN	denotes	surface
T6416	15283-15285	IN	denotes	of
T6417	15286-15288	DT	denotes	an
T6419	15289-15294	JJ	denotes	adult
T6418	15295-15300	NN	denotes	heart
T6420	15300-15301	.	denotes	.
T6421	15301-15520	sentence	denotes	(b) close-up surface view of ventricle of a chimeric heart generated by aggregation of two diploid morulae, one hemizygous for the CK6/ECFP (ECFP+) transgene and the other hemizygous for the YC5/EYFP (EYFP+) transgene.
T6422	15302-15303	-LRB-	denotes	(
T6423	15303-15304	LS	denotes	b
T6425	15304-15305	-RRB-	denotes	)
T6426	15306-15311	NN	denotes	close
T6428	15311-15312	HYPH	denotes	-
T6427	15312-15314	NN	denotes	up
T6429	15315-15322	NN	denotes	surface
T6424	15323-15327	NN	denotes	view
T6430	15328-15330	IN	denotes	of
T6431	15331-15340	NN	denotes	ventricle
T6432	15341-15343	IN	denotes	of
T6433	15344-15345	DT	denotes	a
T6435	15346-15354	JJ	denotes	chimeric
T6434	15355-15360	NN	denotes	heart
T6436	15361-15370	VBN	denotes	generated
T6437	15371-15373	IN	denotes	by
T6438	15374-15385	NN	denotes	aggregation
T6439	15386-15388	IN	denotes	of
T6440	15389-15392	CD	denotes	two
T6442	15393-15400	JJ	denotes	diploid
T6441	15401-15408	NNS	denotes	morulae
T6443	15408-15410	,	denotes	,
T6444	15410-15413	CD	denotes	one
T6445	15414-15424	JJ	denotes	hemizygous
T6446	15425-15428	IN	denotes	for
T6447	15429-15432	DT	denotes	the
T6449	15433-15436	NN	denotes	CK6
T6451	15436-15437	HYPH	denotes	/
T6450	15437-15441	NN	denotes	ECFP
T6452	15442-15443	-LRB-	denotes	(
T6453	15443-15448	JJ	denotes	ECFP+
T6454	15448-15449	-RRB-	denotes	)
T6448	15450-15459	NN	denotes	transgene
T6455	15460-15463	CC	denotes	and
T6456	15464-15467	DT	denotes	the
T6457	15468-15473	JJ	denotes	other
T6458	15474-15484	JJ	denotes	hemizygous
T6459	15485-15488	IN	denotes	for
T6460	15489-15492	DT	denotes	the
T6462	15493-15496	NN	denotes	YC5
T6464	15496-15497	HYPH	denotes	/
T6463	15497-15501	NN	denotes	EYFP
T6465	15502-15503	-LRB-	denotes	(
T6466	15503-15508	JJ	denotes	EYFP+
T6467	15508-15509	-RRB-	denotes	)
T6461	15510-15519	NN	denotes	transgene
T6468	15519-15520	.	denotes	.
T6469	15520-15602	sentence	denotes	The regions of yellow/green and cyan fluorescence are clearly mutually exclusive.
T6470	15521-15524	DT	denotes	The
T6471	15525-15532	NNS	denotes	regions
T6473	15533-15535	IN	denotes	of
T6474	15536-15542	JJ	denotes	yellow
T6476	15542-15543	HYPH	denotes	/
T6475	15543-15548	JJ	denotes	green
T6478	15549-15552	CC	denotes	and
T6479	15553-15557	JJ	denotes	cyan
T6477	15558-15570	NN	denotes	fluorescence
T6472	15571-15574	VBP	denotes	are
T6480	15575-15582	RB	denotes	clearly
T6481	15583-15591	RB	denotes	mutually
T6482	15592-15601	JJ	denotes	exclusive
T6483	15601-15602	.	denotes	.
T6484	15602-15711	sentence	denotes	The striations in the fields of fluorescence represent the proliferative zones present within the ventricle.
T6485	15603-15606	DT	denotes	The
T6486	15607-15617	NNS	denotes	striations
T6488	15618-15620	IN	denotes	in
T6489	15621-15624	DT	denotes	the
T6490	15625-15631	NNS	denotes	fields
T6491	15632-15634	IN	denotes	of
T6492	15635-15647	NN	denotes	fluorescence
T6487	15648-15657	VBP	denotes	represent
T6493	15658-15661	DT	denotes	the
T6495	15662-15675	JJ	denotes	proliferative
T6494	15676-15681	NNS	denotes	zones
T6496	15682-15689	JJ	denotes	present
T6497	15690-15696	IN	denotes	within
T6498	15697-15700	DT	denotes	the
T6499	15701-15710	NN	denotes	ventricle
T6500	15710-15711	.	denotes	.
T6501	15711-15832	sentence	denotes	(c) chimeric pancreas generated by aggregation of a morula hemizygous for the YC5/EYFP transgene with CK6/ECFP ES cells.
T6502	15712-15713	-LRB-	denotes	(
T6503	15713-15714	LS	denotes	c
T6505	15714-15715	-RRB-	denotes	)
T6506	15716-15724	JJ	denotes	chimeric
T6504	15725-15733	NN	denotes	pancreas
T6507	15734-15743	VBN	denotes	generated
T6508	15744-15746	IN	denotes	by
T6509	15747-15758	NN	denotes	aggregation
T6510	15759-15761	IN	denotes	of
T6511	15762-15763	DT	denotes	a
T6512	15764-15770	NN	denotes	morula
T6513	15771-15781	JJ	denotes	hemizygous
T6514	15782-15785	IN	denotes	for
T6515	15786-15789	DT	denotes	the
T6517	15790-15793	NN	denotes	YC5
T6519	15793-15794	HYPH	denotes	/
T6518	15794-15798	NN	denotes	EYFP
T6516	15799-15808	NN	denotes	transgene
T6520	15809-15813	IN	denotes	with
T6521	15814-15817	NN	denotes	CK6
T6523	15817-15818	HYPH	denotes	/
T6522	15818-15822	NN	denotes	ECFP
T6525	15823-15825	NN	denotes	ES
T6524	15826-15831	NNS	denotes	cells
T6526	15831-15832	.	denotes	.
T6527	15832-15933	sentence	denotes	The restricted fields of fluorescence represent the proliferative zones present within the pancreas.
T6528	15833-15836	DT	denotes	The
T6530	15837-15847	JJ	denotes	restricted
T6529	15848-15854	NNS	denotes	fields
T6532	15855-15857	IN	denotes	of
T6533	15858-15870	NN	denotes	fluorescence
T6531	15871-15880	VBP	denotes	represent
T6534	15881-15884	DT	denotes	the
T6536	15885-15898	JJ	denotes	proliferative
T6535	15899-15904	NNS	denotes	zones
T6537	15905-15912	JJ	denotes	present
T6538	15913-15919	IN	denotes	within
T6539	15920-15923	DT	denotes	the
T6540	15924-15932	NN	denotes	pancreas
T6541	15932-15933	.	denotes	.
T6542	15933-15998	sentence	denotes	(d) liver from a double-tagged triple-compartment adult chimera.
T6543	15934-15935	-LRB-	denotes	(
T6544	15935-15936	LS	denotes	d
T6546	15936-15937	-RRB-	denotes	)
T6545	15938-15943	NN	denotes	liver
T6547	15944-15948	IN	denotes	from
T6548	15949-15950	DT	denotes	a
T6550	15951-15957	JJ	denotes	double
T6552	15957-15958	HYPH	denotes	-
T6551	15958-15964	JJ	denotes	tagged
T6553	15965-15971	JJ	denotes	triple
T6555	15971-15972	HYPH	denotes	-
T6554	15972-15983	NN	denotes	compartment
T6556	15984-15989	JJ	denotes	adult
T6549	15990-15997	NN	denotes	chimera
T6557	15997-15998	.	denotes	.
T6558	15998-16166	sentence	denotes	A non-fluorescent morula was aggregated with two clumps of ES cells, one carrying the CK6/ECFP transgene (ECFP+) and the other carrying the YC5/EYFP transgene (EYFP+).
T6559	15999-16000	DT	denotes	A
T6561	16001-16016	JJ	denotes	non-fluorescent
T6560	16017-16023	NN	denotes	morula
T6563	16024-16027	VBD	denotes	was
T6562	16028-16038	VBN	denotes	aggregated
T6564	16039-16043	IN	denotes	with
T6565	16044-16047	CD	denotes	two
T6566	16048-16054	NNS	denotes	clumps
T6567	16055-16057	IN	denotes	of
T6568	16058-16060	NN	denotes	ES
T6569	16061-16066	NNS	denotes	cells
T6570	16066-16068	,	denotes	,
T6571	16068-16071	CD	denotes	one
T6572	16072-16080	VBG	denotes	carrying
T6573	16081-16084	DT	denotes	the
T6575	16085-16088	NN	denotes	CK6
T6577	16088-16089	HYPH	denotes	/
T6576	16089-16093	NN	denotes	ECFP
T6574	16094-16103	NN	denotes	transgene
T6578	16104-16105	-LRB-	denotes	(
T6579	16105-16110	JJ	denotes	ECFP+
T6580	16110-16111	-RRB-	denotes	)
T6581	16112-16115	CC	denotes	and
T6582	16116-16119	DT	denotes	the
T6583	16120-16125	JJ	denotes	other
T6584	16126-16134	VBG	denotes	carrying
T6585	16135-16138	DT	denotes	the
T6587	16139-16142	NN	denotes	YC5
T6589	16142-16143	HYPH	denotes	/
T6588	16143-16147	NN	denotes	EYFP
T6586	16148-16157	NN	denotes	transgene
T6590	16158-16159	-LRB-	denotes	(
T6591	16159-16164	JJ	denotes	EYFP+
T6592	16164-16165	-RRB-	denotes	)
T6593	16165-16166	.	denotes	.
T6594	16166-16246	sentence	denotes	An ECFP+ vessel has clonally proliferated and infiltrated the liver lobe (top).
T6595	16167-16169	DT	denotes	An
T6597	16170-16175	JJ	denotes	ECFP+
T6596	16176-16182	NN	denotes	vessel
T6599	16183-16186	VBZ	denotes	has
T6600	16187-16195	RB	denotes	clonally
T6598	16196-16208	VBN	denotes	proliferated
T6601	16209-16212	CC	denotes	and
T6602	16213-16224	VBN	denotes	infiltrated
T6603	16225-16228	DT	denotes	the
T6605	16229-16234	NN	denotes	liver
T6604	16235-16239	NN	denotes	lobe
T6606	16240-16241	-LRB-	denotes	(
T6607	16241-16244	NN	denotes	top
T6608	16244-16245	-RRB-	denotes	)
T6609	16245-16246	.	denotes	.
T6610	16246-16465	sentence	denotes	Note that unlike in heart and pancreas there appears to be more interspersed blue/cyan vs green/yellow fluorescence present in the liver, possibly reflecting greater cell intermingling during the genesis of this organ.
T6611	16247-16251	VB	denotes	Note
T6612	16252-16256	IN	denotes	that
T6614	16257-16263	IN	denotes	unlike
T6615	16264-16266	IN	denotes	in
T6616	16267-16272	NN	denotes	heart
T6617	16273-16276	CC	denotes	and
T6618	16277-16285	NN	denotes	pancreas
T6619	16286-16291	EX	denotes	there
T6613	16292-16299	VBZ	denotes	appears
T6620	16300-16302	TO	denotes	to
T6621	16303-16305	VB	denotes	be
T6622	16306-16310	RBR	denotes	more
T6623	16311-16323	JJ	denotes	interspersed
T6625	16324-16328	JJ	denotes	blue
T6627	16328-16329	HYPH	denotes	/
T6626	16329-16333	JJ	denotes	cyan
T6628	16334-16336	CC	denotes	vs
T6629	16337-16342	JJ	denotes	green
T6631	16342-16343	HYPH	denotes	/
T6630	16343-16349	JJ	denotes	yellow
T6624	16350-16362	NN	denotes	fluorescence
T6632	16363-16370	JJ	denotes	present
T6633	16371-16373	IN	denotes	in
T6634	16374-16377	DT	denotes	the
T6635	16378-16383	NN	denotes	liver
T6636	16383-16385	,	denotes	,
T6637	16385-16393	RB	denotes	possibly
T6638	16394-16404	VBG	denotes	reflecting
T6639	16405-16412	JJR	denotes	greater
T6641	16413-16417	NN	denotes	cell
T6640	16418-16431	NN	denotes	intermingling
T6642	16432-16438	IN	denotes	during
T6643	16439-16442	DT	denotes	the
T6644	16443-16450	NN	denotes	genesis
T6645	16451-16453	IN	denotes	of
T6646	16454-16458	DT	denotes	this
T6647	16459-16464	NN	denotes	organ
T6648	16464-16465	.	denotes	.
T2695	16479-16483	IN	denotes	From
T2697	16484-16489	DT	denotes	these
T2698	16490-16502	NNS	denotes	observations
T2699	16503-16505	PRP	denotes	we
T2696	16506-16514	VBP	denotes	conclude
T2700	16515-16519	IN	denotes	that
T2702	16520-16523	DT	denotes	the
T2704	16524-16528	JJ	denotes	blue
T2706	16528-16529	HYPH	denotes	-
T2705	16529-16536	VBN	denotes	shifted
T2703	16537-16541	NN	denotes	ECFP
T2707	16541-16543	,	denotes	,
T2708	16543-16546	CC	denotes	and
T2709	16547-16550	DT	denotes	the
T2711	16551-16554	JJ	denotes	red
T2713	16554-16555	HYPH	denotes	-
T2712	16555-16562	VBN	denotes	shifted
T2710	16563-16567	NN	denotes	EYFP
T2714	16568-16576	NNS	denotes	variants
T2715	16577-16579	IN	denotes	of
T2716	16580-16585	NN	denotes	wtGFP
T2701	16586-16589	VBP	denotes	are
T2717	16590-16594	RB	denotes	both
T2718	16595-16603	JJ	denotes	amenable
T2719	16604-16606	IN	denotes	to
T2720	16607-16610	NN	denotes	use
T2721	16611-16613	IN	denotes	in
T2722	16614-16618	NNS	denotes	mice
T2723	16618-16619	.	denotes	.
T2724	16619-16800	sentence	denotes	Also since we have established lines of viable and fertile mice having widespread FP expression we have demonstrated that ECFP and EYFP represent developmentally neutral reporters.
T2725	16620-16624	RB	denotes	Also
T2727	16625-16630	IN	denotes	since
T2729	16631-16633	PRP	denotes	we
T2730	16634-16638	VBP	denotes	have
T2728	16639-16650	VBN	denotes	established
T2731	16651-16656	NNS	denotes	lines
T2732	16657-16659	IN	denotes	of
T2733	16660-16666	JJ	denotes	viable
T2735	16667-16670	CC	denotes	and
T2736	16671-16678	JJ	denotes	fertile
T2734	16679-16683	NNS	denotes	mice
T2737	16684-16690	VBG	denotes	having
T2738	16691-16701	JJ	denotes	widespread
T2740	16702-16704	NN	denotes	FP
T2739	16705-16715	NN	denotes	expression
T2741	16716-16718	PRP	denotes	we
T2742	16719-16723	VBP	denotes	have
T2726	16724-16736	VBN	denotes	demonstrated
T2743	16737-16741	IN	denotes	that
T2745	16742-16746	NN	denotes	ECFP
T2746	16747-16750	CC	denotes	and
T2747	16751-16755	NN	denotes	EYFP
T2744	16756-16765	VBP	denotes	represent
T2748	16766-16781	RB	denotes	developmentally
T2749	16782-16789	JJ	denotes	neutral
T2750	16790-16799	NNS	denotes	reporters
T2751	16799-16800	.	denotes	.
T2752	16800-17119	sentence	denotes	Since we were unable to obtain ES cells, embryos or mice with widespread expression of a RFP (DsRed1) we conclude that this reporter is not developmentally neutral or that its expression cannot be sustained at levels high enough for detection, and as such is not as versatile as other gene-based fluorescent reporters.
T2753	16801-16806	IN	denotes	Since
T2755	16807-16809	PRP	denotes	we
T2754	16810-16814	VBD	denotes	were
T2757	16815-16821	JJ	denotes	unable
T2758	16822-16824	TO	denotes	to
T2759	16825-16831	VB	denotes	obtain
T2760	16832-16834	NN	denotes	ES
T2761	16835-16840	NNS	denotes	cells
T2762	16840-16842	,	denotes	,
T2763	16842-16849	NNS	denotes	embryos
T2764	16850-16852	CC	denotes	or
T2765	16853-16857	NNS	denotes	mice
T2766	16858-16862	IN	denotes	with
T2767	16863-16873	JJ	denotes	widespread
T2768	16874-16884	NN	denotes	expression
T2769	16885-16887	IN	denotes	of
T2770	16888-16889	DT	denotes	a
T2771	16890-16893	NN	denotes	RFP
T2772	16894-16895	-LRB-	denotes	(
T2773	16895-16901	NN	denotes	DsRed1
T2774	16901-16902	-RRB-	denotes	)
T2775	16903-16905	PRP	denotes	we
T2756	16906-16914	VBP	denotes	conclude
T2776	16915-16919	IN	denotes	that
T2778	16920-16924	DT	denotes	this
T2779	16925-16933	NN	denotes	reporter
T2777	16934-16936	VBZ	denotes	is
T2780	16937-16940	RB	denotes	not
T2781	16941-16956	RB	denotes	developmentally
T2782	16957-16964	JJ	denotes	neutral
T2783	16965-16967	CC	denotes	or
T2784	16968-16972	IN	denotes	that
T2786	16973-16976	PRP$	denotes	its
T2787	16977-16987	NN	denotes	expression
T2788	16988-16991	MD	denotes	can
T2789	16991-16994	RB	denotes	not
T2790	16995-16997	VB	denotes	be
T2785	16998-17007	VBN	denotes	sustained
T2791	17008-17010	IN	denotes	at
T2792	17011-17017	NNS	denotes	levels
T2793	17018-17022	JJ	denotes	high
T2794	17023-17029	JJ	denotes	enough
T2795	17030-17033	IN	denotes	for
T2796	17034-17043	NN	denotes	detection
T2797	17043-17045	,	denotes	,
T2798	17045-17048	CC	denotes	and
T2799	17049-17051	IN	denotes	as
T2801	17052-17056	JJ	denotes	such
T2800	17057-17059	VBZ	denotes	is
T2802	17060-17063	RB	denotes	not
T2803	17064-17066	RB	denotes	as
T2804	17067-17076	JJ	denotes	versatile
T2805	17077-17079	IN	denotes	as
T2806	17080-17085	JJ	denotes	other
T2808	17086-17090	NN	denotes	gene
T2810	17090-17091	HYPH	denotes	-
T2809	17091-17096	VBN	denotes	based
T2811	17097-17108	JJ	denotes	fluorescent
T2807	17109-17118	NNS	denotes	reporters
T2812	17118-17119	.	denotes	.
T2813	17119-17400	sentence	denotes	ECFP and EYFP can be distinguished on the basis of their unique non-overlapping spectral profiles, and chimeras comprised of ECFP and EYFP tagged compartments demonstrate that the two reporters can be simultaneously non-invasively visualized within the same embryo or adult organ.
T2814	17120-17124	NN	denotes	ECFP
T2816	17125-17128	CC	denotes	and
T2817	17129-17133	NN	denotes	EYFP
T2818	17134-17137	MD	denotes	can
T2819	17138-17140	VB	denotes	be
T2815	17141-17154	VBN	denotes	distinguished
T2820	17155-17157	IN	denotes	on
T2821	17158-17161	DT	denotes	the
T2822	17162-17167	NN	denotes	basis
T2823	17168-17170	IN	denotes	of
T2824	17171-17176	PRP$	denotes	their
T2826	17177-17183	JJ	denotes	unique
T2827	17184-17199	JJ	denotes	non-overlapping
T2828	17200-17208	JJ	denotes	spectral
T2825	17209-17217	NNS	denotes	profiles
T2829	17217-17219	,	denotes	,
T2830	17219-17222	CC	denotes	and
T2831	17223-17231	NNS	denotes	chimeras
T2833	17232-17241	VBN	denotes	comprised
T2834	17242-17244	IN	denotes	of
T2835	17245-17249	NN	denotes	ECFP
T2837	17250-17253	CC	denotes	and
T2838	17254-17258	NN	denotes	EYFP
T2836	17259-17265	VBN	denotes	tagged
T2839	17266-17278	NNS	denotes	compartments
T2832	17279-17290	VBP	denotes	demonstrate
T2840	17291-17295	IN	denotes	that
T2842	17296-17299	DT	denotes	the
T2844	17300-17303	CD	denotes	two
T2843	17304-17313	NNS	denotes	reporters
T2845	17314-17317	MD	denotes	can
T2846	17318-17320	VB	denotes	be
T2847	17321-17335	RB	denotes	simultaneously
T2848	17336-17350	RB	denotes	non-invasively
T2841	17351-17361	VBN	denotes	visualized
T2849	17362-17368	IN	denotes	within
T2850	17369-17372	DT	denotes	the
T2852	17373-17377	JJ	denotes	same
T2851	17378-17384	NN	denotes	embryo
T2853	17385-17387	CC	denotes	or
T2854	17388-17393	JJ	denotes	adult
T2855	17394-17399	NN	denotes	organ
T2856	17399-17400	.	denotes	.
T2857	17400-17549	sentence	denotes	Therefore it should be possible to use flow cytometry to isolate cells expressing either or both of the reporters from embryos or adult organs [14].
T2858	17401-17410	RB	denotes	Therefore
T2860	17411-17413	PRP	denotes	it
T2861	17414-17420	MD	denotes	should
T2859	17421-17423	VB	denotes	be
T2862	17424-17432	JJ	denotes	possible
T2863	17433-17435	TO	denotes	to
T2864	17436-17439	VB	denotes	use
T2865	17440-17444	NN	denotes	flow
T2866	17445-17454	NN	denotes	cytometry
T2867	17455-17457	TO	denotes	to
T2868	17458-17465	VB	denotes	isolate
T2869	17466-17471	NNS	denotes	cells
T2870	17472-17482	VBG	denotes	expressing
T2871	17483-17489	DT	denotes	either
T2872	17490-17492	CC	denotes	or
T2873	17493-17497	DT	denotes	both
T2874	17498-17500	IN	denotes	of
T2875	17501-17504	DT	denotes	the
T2876	17505-17514	NNS	denotes	reporters
T2877	17515-17519	IN	denotes	from
T2878	17520-17527	NNS	denotes	embryos
T2879	17528-17530	CC	denotes	or
T2880	17531-17536	JJ	denotes	adult
T2881	17537-17543	NNS	denotes	organs
T2882	17544-17545	-LRB-	denotes	[
T2883	17545-17547	CD	denotes	14
T2884	17547-17548	-RRB-	denotes	]
T2885	17548-17549	.	denotes	.
T2886	17549-17680	sentence	denotes	These two reporters can also be used to create pairs of donors and acceptors for in vivo fluorescence energy transfer (FRET) [15].
T2887	17550-17555	DT	denotes	These
T2889	17556-17559	CD	denotes	two
T2888	17560-17569	NNS	denotes	reporters
T2891	17570-17573	MD	denotes	can
T2892	17574-17578	RB	denotes	also
T2893	17579-17581	VB	denotes	be
T2890	17582-17586	VBN	denotes	used
T2894	17587-17589	TO	denotes	to
T2895	17590-17596	VB	denotes	create
T2896	17597-17602	NNS	denotes	pairs
T2897	17603-17605	IN	denotes	of
T2898	17606-17612	NNS	denotes	donors
T2899	17613-17616	CC	denotes	and
T2900	17617-17626	NNS	denotes	acceptors
T2901	17627-17630	IN	denotes	for
T2902	17631-17633	FW	denotes	in
T2903	17634-17638	FW	denotes	vivo
T2905	17639-17651	NN	denotes	fluorescence
T2906	17652-17658	NN	denotes	energy
T2904	17659-17667	NN	denotes	transfer
T2907	17668-17669	-LRB-	denotes	(
T2908	17669-17673	NN	denotes	FRET
T2909	17673-17674	-RRB-	denotes	)
T2910	17675-17676	-LRB-	denotes	[
T2911	17676-17678	CD	denotes	15
T2912	17678-17679	-RRB-	denotes	]
T2913	17679-17680	.	denotes	.
T2914	17680-17982	sentence	denotes	Since the emission profiles of ECFP and EYFP are the least overlapping of all the usable GFP variants, these ECFP and EYFP currently represent the best combination for co-visualization and for use in multi-spectral double-tagged transgenic, chimeric and/or in vivo FRET experiments (reviewed in [16]).
T2915	17681-17686	IN	denotes	Since
T2917	17687-17690	DT	denotes	the
T2919	17691-17699	NN	denotes	emission
T2918	17700-17708	NNS	denotes	profiles
T2920	17709-17711	IN	denotes	of
T2921	17712-17716	NN	denotes	ECFP
T2922	17717-17720	CC	denotes	and
T2923	17721-17725	NN	denotes	EYFP
T2916	17726-17729	VBP	denotes	are
T2925	17730-17733	DT	denotes	the
T2927	17734-17739	RBS	denotes	least
T2926	17740-17751	JJ	denotes	overlapping
T2928	17752-17754	IN	denotes	of
T2929	17755-17758	PDT	denotes	all
T2931	17759-17762	DT	denotes	the
T2932	17763-17769	JJ	denotes	usable
T2933	17770-17773	NN	denotes	GFP
T2930	17774-17782	NNS	denotes	variants
T2934	17782-17784	,	denotes	,
T2935	17784-17789	DT	denotes	these
T2936	17790-17794	NN	denotes	ECFP
T2937	17795-17798	CC	denotes	and
T2938	17799-17803	NN	denotes	EYFP
T2939	17804-17813	RB	denotes	currently
T2924	17814-17823	VBP	denotes	represent
T2940	17824-17827	DT	denotes	the
T2942	17828-17832	JJS	denotes	best
T2941	17833-17844	NN	denotes	combination
T2943	17845-17848	IN	denotes	for
T2944	17849-17865	NN	denotes	co-visualization
T2945	17866-17869	CC	denotes	and
T2946	17870-17873	IN	denotes	for
T2947	17874-17877	NN	denotes	use
T2948	17878-17880	IN	denotes	in
T2949	17881-17895	JJ	denotes	multi-spectral
T2951	17896-17902	JJ	denotes	double
T2953	17902-17903	HYPH	denotes	-
T2952	17903-17909	JJ	denotes	tagged
T2954	17910-17920	JJ	denotes	transgenic
T2955	17920-17922	,	denotes	,
T2956	17922-17930	JJ	denotes	chimeric
T2957	17931-17934	CC	denotes	and
T2958	17934-17935	HYPH	denotes	/
T2959	17935-17937	CC	denotes	or
T2960	17938-17940	FW	denotes	in
T2961	17941-17945	FW	denotes	vivo
T2962	17946-17950	NN	denotes	FRET
T2950	17951-17962	NNS	denotes	experiments
T2963	17963-17964	-LRB-	denotes	(
T2964	17964-17972	VBN	denotes	reviewed
T2965	17973-17975	IN	denotes	in
T2966	17976-17977	-LRB-	denotes	[
T2967	17977-17979	CD	denotes	16
T2968	17979-17980	-RRB-	denotes	]
T2969	17980-17981	-RRB-	denotes	)
T2970	17981-17982	.	denotes	.
T2971	17982-18183	sentence	denotes	These experiments have demonstrated the feasibility of monitoring multiple FP reporters non-invasively within a single animal, and have shown the developmental neutrality of the FP reporters employed.
T2972	17983-17988	DT	denotes	These
T2973	17989-18000	NNS	denotes	experiments
T2975	18001-18005	VBP	denotes	have
T2974	18006-18018	VBN	denotes	demonstrated
T2976	18019-18022	DT	denotes	the
T2977	18023-18034	NN	denotes	feasibility
T2978	18035-18037	IN	denotes	of
T2979	18038-18048	VBG	denotes	monitoring
T2980	18049-18057	JJ	denotes	multiple
T2982	18058-18060	NN	denotes	FP
T2981	18061-18070	NNS	denotes	reporters
T2983	18071-18085	RB	denotes	non-invasively
T2984	18086-18092	IN	denotes	within
T2985	18093-18094	DT	denotes	a
T2987	18095-18101	JJ	denotes	single
T2986	18102-18108	NN	denotes	animal
T2988	18108-18110	,	denotes	,
T2989	18110-18113	CC	denotes	and
T2990	18114-18118	VBP	denotes	have
T2991	18119-18124	VBN	denotes	shown
T2992	18125-18128	DT	denotes	the
T2994	18129-18142	JJ	denotes	developmental
T2993	18143-18153	NN	denotes	neutrality
T2995	18154-18156	IN	denotes	of
T2996	18157-18160	DT	denotes	the
T2998	18161-18163	NN	denotes	FP
T2997	18164-18173	NNS	denotes	reporters
T2999	18174-18182	VBN	denotes	employed
T3000	18182-18183	.	denotes	.
T3001	18183-18375	sentence	denotes	This should pave the way for novel strategies designed to incorporate the use of compound transgenics or tissue recombination or chimeric analyses where several compartments are to be tagged.
T3002	18184-18188	DT	denotes	This
T3004	18189-18195	MD	denotes	should
T3003	18196-18200	VB	denotes	pave
T3005	18201-18204	DT	denotes	the
T3006	18205-18208	NN	denotes	way
T3007	18209-18212	IN	denotes	for
T3008	18213-18218	JJ	denotes	novel
T3009	18219-18229	NNS	denotes	strategies
T3010	18230-18238	VBN	denotes	designed
T3011	18239-18241	TO	denotes	to
T3012	18242-18253	VB	denotes	incorporate
T3013	18254-18257	DT	denotes	the
T3014	18258-18261	NN	denotes	use
T3015	18262-18264	IN	denotes	of
T3016	18265-18273	NN	denotes	compound
T3017	18274-18285	NNS	denotes	transgenics
T3018	18286-18288	CC	denotes	or
T3019	18289-18295	NN	denotes	tissue
T3020	18296-18309	NN	denotes	recombination
T3021	18310-18312	CC	denotes	or
T3022	18313-18321	JJ	denotes	chimeric
T3023	18322-18330	NNS	denotes	analyses
T3024	18331-18336	WRB	denotes	where
T3026	18337-18344	JJ	denotes	several
T3027	18345-18357	NNS	denotes	compartments
T3025	18358-18361	VBP	denotes	are
T3028	18362-18364	TO	denotes	to
T3030	18365-18367	VB	denotes	be
T3029	18368-18374	VBN	denotes	tagged
T3031	18374-18375	.	denotes	.
T3032	18375-18504	sentence	denotes	This technology should provide new possibilities in manipulating the mouse genome, and non-invasively studying the consequences.
T3033	18376-18380	DT	denotes	This
T3034	18381-18391	NN	denotes	technology
T3036	18392-18398	MD	denotes	should
T3035	18399-18406	VB	denotes	provide
T3037	18407-18410	JJ	denotes	new
T3038	18411-18424	NNS	denotes	possibilities
T3039	18425-18427	IN	denotes	in
T3040	18428-18440	VBG	denotes	manipulating
T3041	18441-18444	DT	denotes	the
T3043	18445-18450	NN	denotes	mouse
T3042	18451-18457	NN	denotes	genome
T3044	18457-18459	,	denotes	,
T3045	18459-18462	CC	denotes	and
T3046	18463-18477	RB	denotes	non-invasively
T3047	18478-18486	VBG	denotes	studying
T3048	18487-18490	DT	denotes	the
T3049	18491-18503	NNS	denotes	consequences
T3050	18503-18504	.	denotes	.
T3227	18529-18535	NN	denotes	Vector
T3228	18536-18548	NN	denotes	construction
T3229	18548-18705	sentence	denotes	cDNAs encoding ECFP, EYFP and DsRed were amplified from plasmids pECFP, pEYFP and pDsRed1-N1 (Clontech, Inc.) by PCR using HiFi Taq Polymerase (Invitrogen).
T3230	18549-18554	NNS	denotes	cDNAs
T3232	18555-18563	VBG	denotes	encoding
T3233	18564-18568	NN	denotes	ECFP
T3234	18568-18570	,	denotes	,
T3235	18570-18574	NN	denotes	EYFP
T3236	18575-18578	CC	denotes	and
T3237	18579-18584	VBN	denotes	DsRed
T3238	18585-18589	VBD	denotes	were
T3239	18590-18599	VBN	denotes	amplified
T3240	18600-18604	IN	denotes	from
T3241	18605-18613	NNS	denotes	plasmids
T3242	18614-18619	NN	denotes	pECFP
T3243	18619-18621	,	denotes	,
T3244	18621-18626	NN	denotes	pEYFP
T3245	18627-18630	CC	denotes	and
T3246	18631-18638	NN	denotes	pDsRed1
T3248	18638-18639	HYPH	denotes	-
T3247	18639-18641	NN	denotes	N1
T3249	18642-18643	-LRB-	denotes	(
T3251	18643-18651	NNP	denotes	Clontech
T3252	18651-18653	,	denotes	,
T3250	18653-18657	NNP	denotes	Inc.
T3253	18657-18658	-RRB-	denotes	)
T3254	18659-18661	IN	denotes	by
T3255	18662-18665	NN	denotes	PCR
T3231	18666-18671	VBG	denotes	using
T3256	18672-18676	NNP	denotes	HiFi
T3258	18677-18680	NNP	denotes	Taq
T3257	18681-18691	NN	denotes	Polymerase
T3259	18692-18693	-LRB-	denotes	(
T3260	18693-18703	NN	denotes	Invitrogen
T3261	18703-18704	-RRB-	denotes	)
T3262	18704-18705	.	denotes	.
T3263	18705-18965	sentence	denotes	For ECFP and EYFP primers used were GFP-L (5'-TTG AAT TCG CCA CCA TGG TGA GC) and GFP-R (5'-TTG AAT TCT TAC TTG TAC AGC TCG TCC), for dsRed primers were red-L (5'-TTG AAT TCG CCA CCA TGG TGC GCT C) and red-R (5'-TTG AAT TCT TAC GCT ACA GCT ACA GGA ACA GGT G).
T3264	18706-18709	IN	denotes	For
T3266	18710-18714	NN	denotes	ECFP
T3267	18715-18718	CC	denotes	and
T3268	18719-18723	NN	denotes	EYFP
T3269	18724-18731	NNS	denotes	primers
T3270	18732-18736	VBN	denotes	used
T3265	18737-18741	VBD	denotes	were
T3272	18742-18745	NN	denotes	GFP
T3274	18745-18746	HYPH	denotes	-
T3273	18746-18747	NN	denotes	L
T3275	18748-18749	-LRB-	denotes	(
T3276	18749-18750	CD	denotes	5
T3278	18750-18751	SYM	denotes	'
T3279	18751-18752	HYPH	denotes	-
T3280	18752-18755	NN	denotes	TTG
T3281	18756-18759	NN	denotes	AAT
T3282	18760-18763	NN	denotes	TCG
T3283	18764-18767	NN	denotes	CCA
T3284	18768-18771	NN	denotes	CCA
T3285	18772-18775	NN	denotes	TGG
T3286	18776-18779	NN	denotes	TGA
T3277	18780-18782	NN	denotes	GC
T3287	18782-18783	-RRB-	denotes	)
T3288	18784-18787	CC	denotes	and
T3289	18788-18791	NN	denotes	GFP
T3291	18791-18792	HYPH	denotes	-
T3290	18792-18793	NN	denotes	R
T3292	18794-18795	-LRB-	denotes	(
T3294	18795-18796	NN	denotes	5
T3295	18796-18797	SYM	denotes	'
T3296	18797-18798	HYPH	denotes	-
T3297	18798-18801	NN	denotes	TTG
T3298	18802-18805	NN	denotes	AAT
T3299	18806-18809	NN	denotes	TCT
T3300	18810-18813	NN	denotes	TAC
T3301	18814-18817	NN	denotes	TTG
T3302	18818-18821	NN	denotes	TAC
T3303	18822-18825	NN	denotes	AGC
T3304	18826-18829	NN	denotes	TCG
T3293	18830-18833	NN	denotes	TCC
T3305	18833-18834	-RRB-	denotes	)
T3306	18834-18836	,	denotes	,
T3307	18836-18839	IN	denotes	for
T3308	18840-18845	JJ	denotes	dsRed
T3309	18846-18853	NNS	denotes	primers
T3271	18854-18858	VBD	denotes	were
T3310	18859-18862	NN	denotes	red
T3312	18862-18863	HYPH	denotes	-
T3311	18863-18864	NN	denotes	L
T3313	18865-18866	-LRB-	denotes	(
T3314	18866-18867	CD	denotes	5
T3316	18867-18868	SYM	denotes	'
T3317	18868-18869	HYPH	denotes	-
T3318	18869-18872	NN	denotes	TTG
T3319	18873-18876	NN	denotes	AAT
T3320	18877-18880	NN	denotes	TCG
T3321	18881-18884	NN	denotes	CCA
T3322	18885-18888	NN	denotes	CCA
T3323	18889-18892	NN	denotes	TGG
T3324	18893-18896	NN	denotes	TGC
T3325	18897-18900	NN	denotes	GCT
T3315	18901-18902	NN	denotes	C
T3326	18902-18903	-RRB-	denotes	)
T3327	18904-18907	CC	denotes	and
T3328	18908-18911	NN	denotes	red
T3330	18911-18912	HYPH	denotes	-
T3329	18912-18913	NN	denotes	R
T3331	18914-18915	-LRB-	denotes	(
T3332	18915-18916	CD	denotes	5
T3334	18916-18917	SYM	denotes	'
T3335	18917-18918	HYPH	denotes	-
T3336	18918-18921	NN	denotes	TTG
T3337	18922-18925	NN	denotes	AAT
T3338	18926-18929	NN	denotes	TCT
T3339	18930-18933	NN	denotes	TAC
T3340	18934-18937	NN	denotes	GCT
T3341	18938-18941	NN	denotes	ACA
T3342	18942-18945	NN	denotes	GCT
T3343	18946-18949	NN	denotes	ACA
T3344	18950-18953	NN	denotes	GGA
T3345	18954-18957	NN	denotes	ACA
T3346	18958-18961	NN	denotes	GGT
T3333	18962-18963	NN	denotes	G
T3347	18963-18964	-RRB-	denotes	)
T3348	18964-18965	.	denotes	.
T3349	18965-19117	sentence	denotes	All 5' primers contain a Kozak consensus translation initiation site upstream of the ATG for increased translation efficiency in eukaryotic cells [17].
T3350	18966-18969	DT	denotes	All
T3352	18970-18971	CD	denotes	5
T3353	18971-18972	SYM	denotes	'
T3351	18973-18980	NNS	denotes	primers
T3354	18981-18988	VBP	denotes	contain
T3355	18989-18990	DT	denotes	a
T3357	18991-18996	NNP	denotes	Kozak
T3358	18997-19006	NN	denotes	consensus
T3359	19007-19018	NN	denotes	translation
T3360	19019-19029	NN	denotes	initiation
T3356	19030-19034	NN	denotes	site
T3361	19035-19043	JJ	denotes	upstream
T3362	19044-19046	IN	denotes	of
T3363	19047-19050	DT	denotes	the
T3364	19051-19054	NN	denotes	ATG
T3365	19055-19058	IN	denotes	for
T3366	19059-19068	VBN	denotes	increased
T3368	19069-19080	NN	denotes	translation
T3367	19081-19091	NN	denotes	efficiency
T3369	19092-19094	IN	denotes	in
T3370	19095-19105	JJ	denotes	eukaryotic
T3371	19106-19111	NNS	denotes	cells
T3372	19112-19113	-LRB-	denotes	[
T3373	19113-19115	CD	denotes	17
T3374	19115-19116	-RRB-	denotes	]
T3375	19116-19117	.	denotes	.
T3376	19117-19344	sentence	denotes	To construct vectors driving ubiquitous transgene expression in ES cells and mice, the PCR amplified cDNAs were digested with EcoRI and inserted into the EcoRI sites of pCAGGS [18] to generate pCX-ECFP, pCX-EYFP and pCX-DsRed.
T3377	19118-19120	TO	denotes	To
T3378	19121-19130	VB	denotes	construct
T3380	19131-19138	NNS	denotes	vectors
T3381	19139-19146	VBG	denotes	driving
T3382	19147-19157	JJ	denotes	ubiquitous
T3384	19158-19167	NN	denotes	transgene
T3383	19168-19178	NN	denotes	expression
T3385	19179-19181	IN	denotes	in
T3386	19182-19184	NN	denotes	ES
T3387	19185-19190	NNS	denotes	cells
T3388	19191-19194	CC	denotes	and
T3389	19195-19199	NNS	denotes	mice
T3390	19199-19201	,	denotes	,
T3391	19201-19204	DT	denotes	the
T3393	19205-19208	NN	denotes	PCR
T3394	19209-19218	VBN	denotes	amplified
T3392	19219-19224	NNS	denotes	cDNAs
T3395	19225-19229	VBD	denotes	were
T3379	19230-19238	VBN	denotes	digested
T3396	19239-19243	IN	denotes	with
T3397	19244-19249	NN	denotes	EcoRI
T3398	19250-19253	CC	denotes	and
T3399	19254-19262	VBD	denotes	inserted
T3400	19263-19267	IN	denotes	into
T3401	19268-19271	DT	denotes	the
T3403	19272-19277	NN	denotes	EcoRI
T3402	19278-19283	NNS	denotes	sites
T3404	19284-19286	IN	denotes	of
T3405	19287-19293	NN	denotes	pCAGGS
T3406	19294-19295	-LRB-	denotes	[
T3407	19295-19297	CD	denotes	18
T3408	19297-19298	-RRB-	denotes	]
T3409	19299-19301	TO	denotes	to
T3410	19302-19310	VB	denotes	generate
T3411	19311-19314	NN	denotes	pCX
T3413	19314-19315	HYPH	denotes	-
T3412	19315-19319	NN	denotes	ECFP
T3414	19319-19321	,	denotes	,
T3415	19321-19324	NN	denotes	pCX
T3417	19324-19325	HYPH	denotes	-
T3416	19325-19329	NN	denotes	EYFP
T3418	19330-19333	CC	denotes	and
T3419	19334-19337	NN	denotes	pCX
T3421	19337-19338	HYPH	denotes	-
T3420	19338-19343	NN	denotes	DsRed
T3422	19343-19344	.	denotes	.
T3423	19344-19501	sentence	denotes	pCAGGS contains the chicken beta-actin promoter and first intron coupled to the CMV immediate early enhancer, and rabbit beta-globin polyadenylation signal.
T3424	19345-19351	NN	denotes	pCAGGS
T3425	19352-19360	VBZ	denotes	contains
T3426	19361-19364	DT	denotes	the
T3428	19365-19372	NN	denotes	chicken
T3429	19373-19377	NN	denotes	beta
T3431	19377-19378	HYPH	denotes	-
T3430	19378-19383	NN	denotes	actin
T3427	19384-19392	NN	denotes	promoter
T3432	19393-19396	CC	denotes	and
T3433	19397-19402	JJ	denotes	first
T3434	19403-19409	NN	denotes	intron
T3435	19410-19417	VBN	denotes	coupled
T3436	19418-19420	IN	denotes	to
T3437	19421-19424	DT	denotes	the
T3439	19425-19428	NN	denotes	CMV
T3440	19429-19438	JJ	denotes	immediate
T3441	19439-19444	JJ	denotes	early
T3438	19445-19453	NN	denotes	enhancer
T3442	19453-19455	,	denotes	,
T3443	19455-19458	CC	denotes	and
T3444	19459-19465	NN	denotes	rabbit
T3446	19466-19470	NN	denotes	beta
T3448	19470-19471	HYPH	denotes	-
T3447	19471-19477	NN	denotes	globin
T3449	19478-19493	NN	denotes	polyadenylation
T3445	19494-19500	NN	denotes	signal
T3450	19500-19501	.	denotes	.
T3451	19501-19659	sentence	denotes	This promoter/enhancer combination has previously been shown to drive strong and widespread transgene expression in ES cells, embryos and adult mice [18,19].
T3452	19502-19506	DT	denotes	This
T3454	19507-19515	NN	denotes	promoter
T3456	19515-19516	HYPH	denotes	/
T3455	19516-19524	NN	denotes	enhancer
T3453	19525-19536	NN	denotes	combination
T3458	19537-19540	VBZ	denotes	has
T3459	19541-19551	RB	denotes	previously
T3460	19552-19556	VBN	denotes	been
T3457	19557-19562	VBN	denotes	shown
T3461	19563-19565	TO	denotes	to
T3462	19566-19571	VB	denotes	drive
T3463	19572-19578	JJ	denotes	strong
T3465	19579-19582	CC	denotes	and
T3466	19583-19593	JJ	denotes	widespread
T3467	19594-19603	NN	denotes	transgene
T3464	19604-19614	NN	denotes	expression
T3468	19615-19617	IN	denotes	in
T3469	19618-19620	NN	denotes	ES
T3470	19621-19626	NNS	denotes	cells
T3471	19626-19628	,	denotes	,
T3472	19628-19635	NNS	denotes	embryos
T3473	19636-19639	CC	denotes	and
T3474	19640-19645	JJ	denotes	adult
T3475	19646-19650	NNS	denotes	mice
T3476	19651-19652	-LRB-	denotes	[
T3478	19652-19654	CD	denotes	18
T3479	19654-19655	,	denotes	,
T3477	19655-19657	CD	denotes	19
T3480	19657-19658	-RRB-	denotes	]
T3481	19658-19659	.	denotes	.
T3780	19661-19667	NN	denotes	Tissue
T3781	19668-19675	NN	denotes	culture
T3782	19675-19796	sentence	denotes	R1 ES cells [20] were maintained under standard culture conditions in the presence of LIF, as described previously [21].
T3783	19676-19678	NN	denotes	R1
T3785	19679-19681	NN	denotes	ES
T3784	19682-19687	NNS	denotes	cells
T3787	19688-19689	-LRB-	denotes	[
T3788	19689-19691	CD	denotes	20
T3789	19691-19692	-RRB-	denotes	]
T3790	19693-19697	VBD	denotes	were
T3786	19698-19708	VBN	denotes	maintained
T3791	19709-19714	IN	denotes	under
T3792	19715-19723	JJ	denotes	standard
T3794	19724-19731	NN	denotes	culture
T3793	19732-19742	NNS	denotes	conditions
T3795	19743-19745	IN	denotes	in
T3796	19746-19749	DT	denotes	the
T3797	19750-19758	NN	denotes	presence
T3798	19759-19761	IN	denotes	of
T3799	19762-19765	NN	denotes	LIF
T3800	19765-19767	,	denotes	,
T3801	19767-19769	IN	denotes	as
T3802	19770-19779	VBN	denotes	described
T3803	19780-19790	RB	denotes	previously
T3804	19791-19792	-LRB-	denotes	[
T3805	19792-19794	CD	denotes	21
T3806	19794-19795	-RRB-	denotes	]
T3807	19795-19796	.	denotes	.
T3808	19796-19935	sentence	denotes	20 μg of SalI linearized pCX-ECFP, pCX-EYFP or pCX-DsRed1 was co-electroporated into 2 × 107 cells with 5–10 μg of circular pPGKPuro [22].
T3809	19797-19799	CD	denotes	20
T3810	19800-19802	NNS	denotes	μg
T3812	19803-19805	IN	denotes	of
T3813	19806-19810	NN	denotes	SalI
T3814	19811-19821	VBN	denotes	linearized
T3816	19822-19825	NN	denotes	pCX
T3817	19825-19826	HYPH	denotes	-
T3815	19826-19830	NN	denotes	ECFP
T3818	19830-19832	,	denotes	,
T3819	19832-19835	NN	denotes	pCX
T3821	19835-19836	HYPH	denotes	-
T3820	19836-19840	NN	denotes	EYFP
T3822	19841-19843	CC	denotes	or
T3823	19844-19847	NN	denotes	pCX
T3825	19847-19848	HYPH	denotes	-
T3824	19848-19854	NN	denotes	DsRed1
T3826	19855-19858	VBD	denotes	was
T3811	19859-19876	VBN	denotes	co-electroporated
T3827	19877-19881	IN	denotes	into
T3828	19882-19883	CD	denotes	2
T3830	19884-19885	SYM	denotes	×
T3829	19886-19889	CD	denotes	107
T3831	19890-19895	NNS	denotes	cells
T3832	19896-19900	IN	denotes	with
T3833	19901-19902	CD	denotes	5
T3835	19902-19903	SYM	denotes	–
T3834	19903-19905	CD	denotes	10
T3836	19906-19908	NNS	denotes	μg
T3837	19909-19911	IN	denotes	of
T3838	19912-19920	JJ	denotes	circular
T3839	19921-19929	NN	denotes	pPGKPuro
T3840	19930-19931	-LRB-	denotes	[
T3841	19931-19933	CD	denotes	22
T3842	19933-19934	-RRB-	denotes	]
T3843	19934-19935	.	denotes	.
T3844	19935-20045	sentence	denotes	This provided transient drug resistance which could be used as a selection for obtaining individual colonies.
T3845	19936-19940	DT	denotes	This
T3846	19941-19949	VBN	denotes	provided
T3847	19950-19959	JJ	denotes	transient
T3849	19960-19964	NN	denotes	drug
T3848	19965-19975	NN	denotes	resistance
T3850	19976-19981	WDT	denotes	which
T3852	19982-19987	MD	denotes	could
T3853	19988-19990	VB	denotes	be
T3851	19991-19995	VBN	denotes	used
T3854	19996-19998	IN	denotes	as
T3855	19999-20000	DT	denotes	a
T3856	20001-20010	NN	denotes	selection
T3857	20011-20014	IN	denotes	for
T3858	20015-20024	VBG	denotes	obtaining
T3859	20025-20035	JJ	denotes	individual
T3860	20036-20044	NNS	denotes	colonies
T3861	20044-20045	.	denotes	.
T3862	20045-20190	sentence	denotes	Selection was applied 24 hours after electroporation and maintained for 5 days, thereafter cells were propagated in in the absence of selection.
T3863	20046-20055	NN	denotes	Selection
T3865	20056-20059	VBD	denotes	was
T3864	20060-20067	VBN	denotes	applied
T3867	20068-20070	CD	denotes	24
T3868	20071-20076	NNS	denotes	hours
T3869	20077-20082	IN	denotes	after
T3870	20083-20098	NN	denotes	electroporation
T3871	20099-20102	CC	denotes	and
T3872	20103-20113	VBN	denotes	maintained
T3873	20114-20117	IN	denotes	for
T3874	20118-20119	CD	denotes	5
T3875	20120-20124	NNS	denotes	days
T3876	20124-20126	,	denotes	,
T3877	20126-20136	RB	denotes	thereafter
T3878	20137-20142	NNS	denotes	cells
T3879	20143-20147	VBD	denotes	were
T3866	20148-20158	VBN	denotes	propagated
T3880	20159-20161	RP	denotes	in
T3881	20162-20164	IN	denotes	in
T3882	20165-20168	DT	denotes	the
T3883	20169-20176	NN	denotes	absence
T3884	20177-20179	IN	denotes	of
T3885	20180-20189	NN	denotes	selection
T3886	20189-20190	.	denotes	.
T3887	20190-20296	sentence	denotes	Fluorescent colonies were picked into 96-well plates, expanded and archived as described previously ([2].
T3888	20191-20202	JJ	denotes	Fluorescent
T3889	20203-20211	NNS	denotes	colonies
T3891	20212-20216	VBD	denotes	were
T3890	20217-20223	VBN	denotes	picked
T3892	20224-20228	IN	denotes	into
T3893	20229-20231	CD	denotes	96
T3895	20231-20232	HYPH	denotes	-
T3894	20232-20236	NN	denotes	well
T3896	20237-20243	NNS	denotes	plates
T3897	20243-20245	,	denotes	,
T3898	20245-20253	VBD	denotes	expanded
T3899	20254-20257	CC	denotes	and
T3900	20258-20266	VBD	denotes	archived
T3901	20267-20269	IN	denotes	as
T3902	20270-20279	VBN	denotes	described
T3903	20280-20290	RB	denotes	previously
T3904	20291-20292	-LRB-	denotes	(
T3905	20292-20293	-LRB-	denotes	[
T3906	20293-20294	CD	denotes	2
T3907	20294-20295	-RRB-	denotes	]
T3908	20295-20296	.	denotes	.
T3909	20296-20422	sentence	denotes	Clones were screened for strong, stable, and homogenous transgene expression during culture in the absence of drug selection.
T3910	20297-20303	NNS	denotes	Clones
T3912	20304-20308	VBD	denotes	were
T3911	20309-20317	VBN	denotes	screened
T3913	20318-20321	IN	denotes	for
T3914	20322-20328	JJ	denotes	strong
T3916	20328-20330	,	denotes	,
T3917	20330-20336	JJ	denotes	stable
T3918	20336-20338	,	denotes	,
T3919	20338-20341	CC	denotes	and
T3920	20342-20352	JJ	denotes	homogenous
T3921	20353-20362	NN	denotes	transgene
T3915	20363-20373	NN	denotes	expression
T3922	20374-20380	IN	denotes	during
T3923	20381-20388	NN	denotes	culture
T3924	20389-20391	IN	denotes	in
T3925	20392-20395	DT	denotes	the
T3926	20396-20403	NN	denotes	absence
T3927	20404-20406	IN	denotes	of
T3928	20407-20411	NN	denotes	drug
T3929	20412-20421	NN	denotes	selection
T3930	20421-20422	.	denotes	.
T4217	20424-20431	NN	denotes	Chimera
T4218	20432-20442	NN	denotes	production
T4219	20443-20446	CC	denotes	and
T4220	20447-20455	NN	denotes	germline
T4221	20456-20468	NN	denotes	transmission
T4222	20468-20800	sentence	denotes	ES cell lines exhibiting robust transgene expression in vitro were assessed in vivo for the spatiotemporal extent of transgene expression and their developmental potential in chimeras produced by aggregation with wild type tetraploid ICR embryos so as to produce completely ES cell-derived embryos, as described previously [20,23].
T4223	20469-20471	NN	denotes	ES
T4224	20472-20476	NN	denotes	cell
T4225	20477-20482	NNS	denotes	lines
T4227	20483-20493	VBG	denotes	exhibiting
T4228	20494-20500	JJ	denotes	robust
T4230	20501-20510	NN	denotes	transgene
T4229	20511-20521	NN	denotes	expression
T4231	20522-20524	FW	denotes	in
T4232	20525-20530	FW	denotes	vitro
T4233	20531-20535	VBD	denotes	were
T4226	20536-20544	VBN	denotes	assessed
T4234	20545-20547	FW	denotes	in
T4235	20548-20552	FW	denotes	vivo
T4236	20553-20556	IN	denotes	for
T4237	20557-20560	DT	denotes	the
T4239	20561-20575	JJ	denotes	spatiotemporal
T4238	20576-20582	NN	denotes	extent
T4240	20583-20585	IN	denotes	of
T4241	20586-20595	NN	denotes	transgene
T4242	20596-20606	NN	denotes	expression
T4243	20607-20610	CC	denotes	and
T4244	20611-20616	PRP$	denotes	their
T4246	20617-20630	JJ	denotes	developmental
T4245	20631-20640	NN	denotes	potential
T4247	20641-20643	IN	denotes	in
T4248	20644-20652	NNS	denotes	chimeras
T4249	20653-20661	VBN	denotes	produced
T4250	20662-20664	IN	denotes	by
T4251	20665-20676	NN	denotes	aggregation
T4252	20677-20681	IN	denotes	with
T4253	20682-20686	JJ	denotes	wild
T4254	20687-20691	NN	denotes	type
T4256	20692-20702	JJ	denotes	tetraploid
T4257	20703-20706	NN	denotes	ICR
T4255	20707-20714	NNS	denotes	embryos
T4258	20715-20717	RB	denotes	so
T4260	20718-20720	IN	denotes	as
T4261	20721-20723	TO	denotes	to
T4259	20724-20731	VB	denotes	produce
T4262	20732-20742	RB	denotes	completely
T4264	20743-20745	VBZ	denotes	ES
T4265	20746-20750	JJ	denotes	cell
T4266	20750-20751	HYPH	denotes	-
T4263	20751-20758	JJ	denotes	derived
T4267	20759-20766	NNS	denotes	embryos
T4268	20766-20768	,	denotes	,
T4269	20768-20770	IN	denotes	as
T4270	20771-20780	VBN	denotes	described
T4271	20781-20791	RB	denotes	previously
T4272	20792-20793	-LRB-	denotes	[
T4274	20793-20795	CD	denotes	20
T4275	20795-20796	,	denotes	,
T4273	20796-20798	CD	denotes	23
T4276	20798-20799	-RRB-	denotes	]
T4277	20799-20800	.	denotes	.
T4278	20800-20860	sentence	denotes	A detailed protocol can be found on the world wide web at .
T4279	20801-20802	DT	denotes	A
T4281	20803-20811	JJ	denotes	detailed
T4280	20812-20820	NN	denotes	protocol
T4283	20821-20824	MD	denotes	can
T4284	20825-20827	VB	denotes	be
T4282	20828-20833	VBN	denotes	found
T4285	20834-20836	IN	denotes	on
T4286	20837-20840	DT	denotes	the
T4288	20841-20846	NN	denotes	world
T4289	20847-20851	JJ	denotes	wide
T4287	20852-20855	NN	denotes	web
T4290	20856-20858	IN	denotes	at
T4291	20859-20860	.	denotes	.
T4292	20860-21081	sentence	denotes	Clones giving fluorescent E12.5 embryos having no readily identifiable malformations were used to generate germline transmitting chimeras by aggregation with diploid wild type ICR embryos as described previously [21,23].
T4293	20861-20867	NNS	denotes	Clones
T4295	20868-20874	VBG	denotes	giving
T4296	20875-20886	JJ	denotes	fluorescent
T4298	20887-20892	NN	denotes	E12.5
T4297	20893-20900	NNS	denotes	embryos
T4299	20901-20907	VBG	denotes	having
T4300	20908-20910	RB	denotes	no
T4302	20911-20918	RB	denotes	readily
T4303	20919-20931	JJ	denotes	identifiable
T4301	20932-20945	NNS	denotes	malformations
T4304	20946-20950	VBD	denotes	were
T4294	20951-20955	VBN	denotes	used
T4305	20956-20958	TO	denotes	to
T4306	20959-20967	VB	denotes	generate
T4307	20968-20976	NN	denotes	germline
T4308	20977-20989	VBG	denotes	transmitting
T4309	20990-20998	NNS	denotes	chimeras
T4310	20999-21001	IN	denotes	by
T4311	21002-21013	NN	denotes	aggregation
T4312	21014-21018	IN	denotes	with
T4313	21019-21026	JJ	denotes	diploid
T4315	21027-21031	JJ	denotes	wild
T4316	21032-21036	NN	denotes	type
T4317	21037-21040	NN	denotes	ICR
T4314	21041-21048	NNS	denotes	embryos
T4318	21049-21051	IN	denotes	as
T4319	21052-21061	VBN	denotes	described
T4320	21062-21072	RB	denotes	previously
T4321	21073-21074	-LRB-	denotes	[
T4323	21074-21076	CD	denotes	21
T4324	21076-21077	,	denotes	,
T4322	21077-21079	CD	denotes	23
T4325	21079-21080	-RRB-	denotes	]
T4326	21080-21081	.	denotes	.
T4327	21081-21147	sentence	denotes	After germline transmission transgenes were bred to homozygosity.
T4328	21082-21087	IN	denotes	After
T4330	21088-21096	NN	denotes	germline
T4331	21097-21109	NN	denotes	transmission
T4332	21110-21120	NNS	denotes	transgenes
T4333	21121-21125	VBD	denotes	were
T4329	21126-21130	VBN	denotes	bred
T4334	21131-21133	IN	denotes	to
T4335	21134-21146	NN	denotes	homozygosity
T4336	21146-21147	.	denotes	.
T4337	21147-21241	sentence	denotes	Transgenes were maintained on both mixedbred ICR and inbred 129/SvJ genetic backgrounds [24].
T4338	21148-21158	NNS	denotes	Transgenes
T4340	21159-21163	VBD	denotes	were
T4339	21164-21174	VBN	denotes	maintained
T4341	21175-21177	IN	denotes	on
T4342	21178-21182	CC	denotes	both
T4344	21183-21192	JJ	denotes	mixedbred
T4343	21193-21196	NN	denotes	ICR
T4346	21197-21200	CC	denotes	and
T4347	21201-21207	VBD	denotes	inbred
T4349	21208-21211	NN	denotes	129
T4350	21211-21212	HYPH	denotes	/
T4348	21212-21215	NN	denotes	SvJ
T4351	21216-21223	JJ	denotes	genetic
T4345	21224-21235	NNS	denotes	backgrounds
T4352	21236-21237	-LRB-	denotes	[
T4353	21237-21239	CD	denotes	24
T4354	21239-21240	-RRB-	denotes	]
T4355	21240-21241	.	denotes	.
T4639	21243-21250	NN	denotes	Imaging
T4640	21250-21407	sentence	denotes	Photographs of live samples, freshly dissected in PBS ([24] were taken on a Leica MZFLIII stereo dissecting microscope equipped with epifluorescence optics.
T4641	21251-21262	NNS	denotes	Photographs
T4643	21263-21265	IN	denotes	of
T4644	21266-21270	JJ	denotes	live
T4645	21271-21278	NNS	denotes	samples
T4646	21278-21280	,	denotes	,
T4647	21280-21287	RB	denotes	freshly
T4648	21288-21297	VBN	denotes	dissected
T4649	21298-21300	IN	denotes	in
T4650	21301-21304	NNP	denotes	PBS
T4651	21305-21306	-LRB-	denotes	(
T4653	21306-21307	-LRB-	denotes	[
T4652	21307-21309	CD	denotes	24
T4654	21309-21310	-RRB-	denotes	]
T4655	21311-21315	VBD	denotes	were
T4642	21316-21321	VBN	denotes	taken
T4656	21322-21324	IN	denotes	on
T4657	21325-21326	DT	denotes	a
T4659	21327-21332	NNP	denotes	Leica
T4660	21333-21340	NNP	denotes	MZFLIII
T4661	21341-21347	NN	denotes	stereo
T4662	21348-21358	NN	denotes	dissecting
T4658	21359-21369	NN	denotes	microscope
T4663	21370-21378	VBN	denotes	equipped
T4664	21379-21383	IN	denotes	with
T4665	21384-21399	NN	denotes	epifluorescence
T4666	21400-21406	NNS	denotes	optics
T4667	21406-21407	.	denotes	.
T4668	21407-21653	sentence	denotes	Filter sets used were: excitation 436/20 nm, dichroic mirror 455 nm, barrier 480/40 nm for ECFP, excitation 500/20 nm, dichroic mirror 515 nm, barrier 535/30 nm for EYFP and excitation 540/25 nm, dichroic mirror 565 nm, barrier 605/55 for DsRed.
T4669	21408-21414	NNP	denotes	Filter
T4670	21415-21419	NNS	denotes	sets
T4672	21420-21424	VBN	denotes	used
T4671	21425-21429	VBD	denotes	were
T4673	21429-21431	:	denotes	:
T4674	21431-21441	NN	denotes	excitation
T4676	21442-21445	CD	denotes	436
T4678	21445-21446	HYPH	denotes	/
T4677	21446-21448	CD	denotes	20
T4675	21449-21451	NNS	denotes	nm
T4679	21451-21453	,	denotes	,
T4680	21453-21461	JJ	denotes	dichroic
T4682	21462-21468	NN	denotes	mirror
T4683	21469-21472	CD	denotes	455
T4681	21473-21475	NNS	denotes	nm
T4684	21475-21477	,	denotes	,
T4685	21477-21484	NN	denotes	barrier
T4687	21485-21488	CD	denotes	480
T4689	21488-21489	HYPH	denotes	/
T4688	21489-21491	CD	denotes	40
T4686	21492-21494	NNS	denotes	nm
T4690	21495-21498	IN	denotes	for
T4691	21499-21503	NN	denotes	ECFP
T4692	21503-21505	,	denotes	,
T4693	21505-21515	NN	denotes	excitation
T4695	21516-21519	CD	denotes	500
T4697	21519-21520	HYPH	denotes	/
T4696	21520-21522	CD	denotes	20
T4694	21523-21525	NN	denotes	nm
T4698	21525-21527	,	denotes	,
T4699	21527-21535	JJ	denotes	dichroic
T4701	21536-21542	NN	denotes	mirror
T4702	21543-21546	CD	denotes	515
T4700	21547-21549	NNS	denotes	nm
T4703	21549-21551	,	denotes	,
T4704	21551-21558	NN	denotes	barrier
T4706	21559-21562	CD	denotes	535
T4708	21562-21563	HYPH	denotes	/
T4707	21563-21565	CD	denotes	30
T4705	21566-21568	NNS	denotes	nm
T4709	21569-21572	IN	denotes	for
T4710	21573-21577	NN	denotes	EYFP
T4711	21578-21581	CC	denotes	and
T4712	21582-21592	NN	denotes	excitation
T4714	21593-21596	CD	denotes	540
T4716	21596-21597	HYPH	denotes	/
T4715	21597-21599	CD	denotes	25
T4713	21600-21602	NNS	denotes	nm
T4717	21602-21604	,	denotes	,
T4718	21604-21612	JJ	denotes	dichroic
T4720	21613-21619	NN	denotes	mirror
T4721	21620-21623	CD	denotes	565
T4719	21624-21626	NNS	denotes	nm
T4722	21626-21628	,	denotes	,
T4723	21628-21635	NN	denotes	barrier
T4724	21636-21639	CD	denotes	605
T4725	21639-21640	HYPH	denotes	/
T4726	21640-21642	CD	denotes	55
T4727	21643-21646	IN	denotes	for
T4728	21647-21652	NN	denotes	DsRed
T4729	21652-21653	.	denotes	.
T4730	21653-21697	sentence	denotes	Adult mice were anaesthetized with avertin.
T4731	21654-21659	NNP	denotes	Adult
T4732	21660-21664	NNS	denotes	mice
T4734	21665-21669	VBD	denotes	were
T4733	21670-21683	VBN	denotes	anaesthetized
T4735	21684-21688	IN	denotes	with
T4736	21689-21696	NN	denotes	avertin
T4737	21696-21697	.	denotes	.
T4738	21697-21921	sentence	denotes	They were then placed on the stage of a stereo dissecting microscope (Leica MZFLIII) from which the objective had been removed, such that they were illuminated using the epifluorescence optics (lamp and excitation filters).
T4739	21698-21702	PRP	denotes	They
T4741	21703-21707	VBD	denotes	were
T4742	21708-21712	RB	denotes	then
T4740	21713-21719	VBN	denotes	placed
T4743	21720-21722	IN	denotes	on
T4744	21723-21726	DT	denotes	the
T4745	21727-21732	NN	denotes	stage
T4746	21733-21735	IN	denotes	of
T4747	21736-21737	DT	denotes	a
T4749	21738-21744	NN	denotes	stereo
T4750	21745-21755	VBG	denotes	dissecting
T4748	21756-21766	NN	denotes	microscope
T4751	21767-21768	-LRB-	denotes	(
T4752	21768-21773	NNP	denotes	Leica
T4753	21774-21781	NNP	denotes	MZFLIII
T4754	21781-21782	-RRB-	denotes	)
T4755	21783-21787	IN	denotes	from
T4757	21788-21793	WDT	denotes	which
T4758	21794-21797	DT	denotes	the
T4759	21798-21807	NN	denotes	objective
T4760	21808-21811	VBD	denotes	had
T4761	21812-21816	VBN	denotes	been
T4756	21817-21824	VBN	denotes	removed
T4762	21824-21826	,	denotes	,
T4763	21826-21830	JJ	denotes	such
T4765	21831-21835	IN	denotes	that
T4766	21836-21840	PRP	denotes	they
T4767	21841-21845	VBD	denotes	were
T4764	21846-21857	VBN	denotes	illuminated
T4768	21858-21863	VBG	denotes	using
T4769	21864-21867	DT	denotes	the
T4771	21868-21883	NN	denotes	epifluorescence
T4770	21884-21890	NNS	denotes	optics
T4772	21891-21892	-LRB-	denotes	(
T4773	21892-21896	NN	denotes	lamp
T4775	21897-21900	CC	denotes	and
T4776	21901-21911	NN	denotes	excitation
T4774	21912-21919	NNS	denotes	filters
T4777	21919-21920	-RRB-	denotes	)
T4778	21920-21921	.	denotes	.
T4779	21921-22021	sentence	denotes	Photographs were taken using a Nikon FE10 35 mm SLR camera fitted with individual emission filters.
T4780	21922-21933	NNS	denotes	Photographs
T4782	21934-21938	VBD	denotes	were
T4781	21939-21944	VBN	denotes	taken
T4783	21945-21950	VBG	denotes	using
T4784	21951-21952	DT	denotes	a
T4786	21953-21958	NNP	denotes	Nikon
T4787	21959-21963	NN	denotes	FE10
T4788	21964-21966	CD	denotes	35
T4789	21967-21969	NN	denotes	mm
T4790	21970-21973	NN	denotes	SLR
T4785	21974-21980	NN	denotes	camera
T4791	21981-21987	VBN	denotes	fitted
T4792	21988-21992	IN	denotes	with
T4793	21993-22003	JJ	denotes	individual
T4795	22004-22012	NN	denotes	emission
T4794	22013-22020	NNS	denotes	filters
T4796	22020-22021	.	denotes	.
T4797	22021-22085	sentence	denotes	Images were processed using Photoshop software (Adobe Systems).
T4798	22022-22028	NNS	denotes	Images
T4800	22029-22033	VBD	denotes	were
T4801	22034-22043	VBN	denotes	processed
T4799	22044-22049	VBG	denotes	using
T4802	22050-22059	NN	denotes	Photoshop
T4803	22060-22068	NN	denotes	software
T4804	22069-22070	-LRB-	denotes	(
T4806	22070-22075	NNP	denotes	Adobe
T4805	22076-22083	NNP	denotes	Systems
T4807	22083-22084	-RRB-	denotes	)
T4808	22084-22085	.	denotes	.
T4927	22087-22095	NNP	denotes	Footnote
T4928	22095-22262	sentence	denotes	The cyan and yellow fluorescent protein-expressing mice described here have been acquired by, and are available from, the Jackson Laboratory Induced Mutant Resource .
T4929	22096-22099	DT	denotes	The
T4931	22100-22104	JJ	denotes	cyan
T4933	22105-22108	CC	denotes	and
T4934	22109-22115	JJ	denotes	yellow
T4935	22116-22127	JJ	denotes	fluorescent
T4932	22128-22135	NN	denotes	protein
T4937	22135-22136	HYPH	denotes	-
T4936	22136-22146	VBG	denotes	expressing
T4930	22147-22151	NNS	denotes	mice
T4939	22152-22161	VBN	denotes	described
T4940	22162-22166	RB	denotes	here
T4941	22167-22171	VBP	denotes	have
T4942	22172-22176	VBN	denotes	been
T4938	22177-22185	VBN	denotes	acquired
T4943	22186-22188	IN	denotes	by
T4944	22188-22190	,	denotes	,
T4945	22190-22193	CC	denotes	and
T4946	22194-22197	VBP	denotes	are
T4947	22198-22207	JJ	denotes	available
T4948	22208-22212	IN	denotes	from
T4949	22212-22214	,	denotes	,
T4950	22214-22217	DT	denotes	the
T4952	22218-22225	NNP	denotes	Jackson
T4953	22226-22236	NNP	denotes	Laboratory
T4954	22237-22244	NNP	denotes	Induced
T4955	22245-22251	NNP	denotes	Mutant
T4951	22252-22260	NNP	denotes	Resource
T4956	22261-22262	.	denotes	.
T4957	22262-22386	sentence	denotes	They are referred to therein as 003373 Stock TgN(ActbECFP) 1Nagy (CK6/ECFP) and 003772 Stock TgN(ActbEYFP)1Nagy (YC5/EYFP).
T4958	22263-22267	PRP	denotes	They
T4960	22268-22271	VBP	denotes	are
T4959	22272-22280	VBN	denotes	referred
T4961	22281-22283	IN	denotes	to
T4962	22284-22291	RB	denotes	therein
T4963	22292-22294	IN	denotes	as
T4964	22295-22301	CD	denotes	003373
T4965	22302-22307	NNP	denotes	Stock
T4966	22308-22311	NNP	denotes	TgN
T4967	22311-22312	-LRB-	denotes	(
T4968	22312-22320	NN	denotes	ActbECFP
T4969	22320-22321	-RRB-	denotes	)
T4970	22322-22327	NN	denotes	1Nagy
T4971	22328-22329	-LRB-	denotes	(
T4972	22329-22332	NN	denotes	CK6
T4974	22332-22333	HYPH	denotes	/
T4973	22333-22337	NN	denotes	ECFP
T4975	22337-22338	-RRB-	denotes	)
T4976	22339-22342	CC	denotes	and
T4977	22343-22349	CD	denotes	003772
T4978	22350-22355	NN	denotes	Stock
T4979	22356-22359	NN	denotes	TgN
T4980	22359-22360	-LRB-	denotes	(
T4981	22360-22368	NN	denotes	ActbEYFP
T4982	22368-22369	-RRB-	denotes	)
T4983	22369-22374	NN	denotes	1Nagy
T4984	22375-22376	-LRB-	denotes	(
T4985	22376-22379	NN	denotes	YC5
T4987	22379-22380	HYPH	denotes	/
T4986	22380-22384	NN	denotes	EYFP
T4988	22384-22385	-RRB-	denotes	)
T4989	22385-22386	.	denotes	.
R10	T161	T158	cc	and,cells
R100	T261	T262	nsubj	We,demonstrated
R1000	T1713	T1711	conj	EYFP,ECFP
R1001	T1714	T1706	auxpass	were,obtained
R1002	T1715	T1706	prep	at,obtained
R1003	T1716	T1717	det	the,frequency
R1004	T1717	T1715	pobj	frequency,at
R1005	T1718	T1717	amod	same,frequency
R1006	T1719	T1720	mark	as,obtained
R1007	T1720	T1717	advcl	obtained,frequency
R1008	T1721	T1720	nsubj	we,obtained
R1009	T1722	T1720	aux	had,obtained
R101	T263	T262	aux	have,demonstrated
R1010	T1723	T1720	advmod	previously,obtained
R1011	T1724	T1720	prep	for,obtained
R1012	T1725	T1724	pobj	EGFP,for
R1013	T1726	T1706	punct	.,obtained
R1014	T1728	T1729	nsubj	Cells,were
R1015	T1730	T1728	acl	expressing,Cells
R1016	T1731	T1732	nummod	one,other
R1017	T1732	T1730	dobj	other,expressing
R1018	T1733	T1732	cc	or,other
R1019	T1734	T1732	prep	of,other
R102	T264	T262	advmod	previously,demonstrated
R1020	T1735	T1736	det	the,reporters
R1021	T1736	T1734	pobj	reporters,of
R1022	T1737	T1736	nummod	two,reporters
R1023	T1738	T1736	nmod	GFP,reporters
R1024	T1739	T1738	amod	variant,GFP
R1025	T1740	T1741	advmod	clearly,distinguishable
R1026	T1741	T1729	acomp	distinguishable,were
R1027	T1742	T1729	prep	within,were
R1028	T1743	T1744	det	a,population
R1029	T1744	T1742	pobj	population,within
R103	T265	T266	det	the,utility
R1030	T1745	T1744	amod	mixed,population
R1031	T1746	T1747	punct	(,1d
R1032	T1747	T1729	parataxis	1d,were
R1033	T1748	T1747	nmod	Fig.,1d
R1034	T1749	T1747	nmod	1a,1d
R1035	T1750	T1747	punct	",",1d
R1036	T1751	T1747	nmod	1b,1d
R1037	T1752	T1747	punct	",",1d
R1038	T1753	T1747	nmod	1c,1d
R1039	T1754	T1747	punct	",",1d
R104	T266	T262	dobj	utility,demonstrated
R1040	T1755	T1747	punct	),1d
R1041	T1756	T1729	punct	.,were
R1042	T1758	T1759	advmod	Interestingly,were
R1043	T1760	T1759	advmod	however,were
R1044	T1761	T1759	nsubj	we,were
R1045	T1762	T1759	acomp	unable,were
R1046	T1763	T1764	aux	to,recover
R1047	T1764	T1762	xcomp	recover,unable
R1048	T1765	T1764	dobj	clones,recover
R1049	T1766	T1765	acl	expressing,clones
R105	T267	T266	cc	and,utility
R1050	T1767	T1766	dobj	DsRed1,expressing
R1051	T1768	T1766	prep	at,expressing
R1052	T1769	T1770	det	the,frequency
R1053	T1770	T1768	pobj	frequency,at
R1054	T1771	T1770	amod	same,frequency
R1055	T1772	T1770	prep	as,frequency
R1056	T1773	T1772	prep	for,as
R1057	T1774	T1775	det	the,reporters
R1058	T1775	T1773	pobj	reporters,for
R1059	T1776	T1775	nmod	GFP,reporters
R106	T268	T269	amod	developmental,neutrality
R1060	T1777	T1776	amod	variant,GFP
R1061	T1778	T1759	prep	after,were
R1062	T1779	T1778	pobj	electroporation,after
R1063	T1780	T1779	cc	and,electroporation
R1064	T1781	T1779	conj	selection,electroporation
R1065	T1782	T1779	prep	under,electroporation
R1066	T1783	T1784	amod	identical,conditions
R1067	T1784	T1782	pobj	conditions,under
R1068	T1785	T1759	punct	.,were
R1069	T1787	T1788	advmod	Indeed,retain
R107	T269	T266	conj	neutrality,utility
R1070	T1789	T1790	det	the,clones
R1071	T1790	T1788	nsubj	clones,retain
R1072	T1791	T1792	npadvmod	DsRed1,expressing
R1073	T1792	T1790	amod	expressing,clones
R1074	T1793	T1794	dep	that,were
R1075	T1794	T1790	relcl	were,clones
R1076	T1795	T1794	nsubj	we,were
R1077	T1796	T1794	acomp	able,were
R1078	T1797	T1798	aux	to,recover
R1079	T1798	T1796	xcomp	recover,able
R108	T270	T266	prep	of,utility
R1080	T1799	T1788	aux	did,retain
R1081	T1800	T1788	neg	not,retain
R1082	T1801	T1802	poss	their,morphology
R1083	T1802	T1788	dobj	morphology,retain
R1084	T1803	T1802	amod	characteristic,morphology
R1085	T1804	T1805	compound	ES,cell
R1086	T1805	T1802	compound	cell,morphology
R1087	T1806	T1788	punct	", ",retain
R1088	T1807	T1808	advmod	usually,appearing
R1089	T1808	T1788	advcl	appearing,retain
R109	T271	T272	amod	enhanced,protein
R1090	T1809	T1808	oprd	smaller,appearing
R1091	T1810	T1809	cc	and,smaller
R1092	T1811	T1812	advmod	more,spherical
R1093	T1812	T1809	conj	spherical,smaller
R1094	T1813	T1814	punct	(,1e
R1095	T1814	T1809	parataxis	1e,smaller
R1096	T1815	T1814	compound	Fig.,1e
R1097	T1816	T1814	cc	and,1e
R1098	T1817	T1814	conj	1f,1e
R1099	T1818	T1814	punct	),1e
R11	T162	T158	conj	mice,cells
R110	T272	T270	pobj	protein,of
R1100	T1819	T1809	punct	", ",smaller
R1101	T1820	T1809	cc	or,smaller
R1102	T1821	T1822	advmod	occasionally,shaped
R1103	T1822	T1809	conj	shaped,smaller
R1104	T1823	T1822	npadvmod	spindle,shaped
R1105	T1824	T1825	punct	(,shown
R1106	T1825	T1788	parataxis	shown,retain
R1107	T1826	T1825	nsubj	data,shown
R1108	T1827	T1825	neg	not,shown
R1109	T1828	T1825	punct	),shown
R111	T273	T272	amod	green,protein
R1110	T1829	T1788	punct	.,retain
R1111	T1831	T1832	prep	Unlike,was
R1112	T1833	T1834	det	the,fluorescence
R1113	T1834	T1831	pobj	fluorescence,Unlike
R1114	T1835	T1834	acl	produced,fluorescence
R1115	T1836	T1835	agent	by,produced
R1116	T1837	T1838	det	the,variants
R1117	T1838	T1836	pobj	variants,by
R1118	T1839	T1838	compound	GFP,variants
R1119	T1840	T1841	dep	which,distributed
R112	T274	T272	amod	fluorescent,protein
R1120	T1841	T1834	relcl	distributed,fluorescence
R1121	T1842	T1841	auxpass	was,distributed
R1122	T1843	T1841	advmod	evenly,distributed
R1123	T1844	T1841	prep	throughout,distributed
R1124	T1845	T1846	det	the,cells
R1125	T1846	T1844	pobj	cells,throughout
R1126	T1847	T1832	punct	", ",was
R1127	T1848	T1849	det	the,signal
R1128	T1849	T1832	nsubj	signal,was
R1129	T1850	T1849	compound	DsRed,signal
R113	T275	T272	punct	(,protein
R1130	T1851	T1832	neg	never,was
R1131	T1852	T1832	acomp	homogenous,was
R1132	T1853	T1832	punct	.,was
R1133	T1855	T1856	amod	Red,fluorescence
R1134	T1856	T1857	nsubjpass	fluorescence,aggregated
R1135	T1858	T1857	auxpass	was,aggregated
R1136	T1859	T1857	advmod	often,aggregated
R1137	T1860	T1857	prep	in,aggregated
R1138	T1861	T1862	dep	what,appeared
R1139	T1862	T1860	pcomp	appeared,in
R114	T276	T272	appos	EGFP,protein
R1140	T1863	T1864	aux	to,be
R1141	T1864	T1862	xcomp	be,appeared
R1142	T1865	T1866	amod	perinuclear,regions
R1143	T1866	T1864	attr	regions,be
R1144	T1867	T1866	prep	of,regions
R1145	T1868	T1869	det	the,cytoplasm
R1146	T1869	T1867	pobj	cytoplasm,of
R1147	T1870	T1857	punct	.,aggregated
R1148	T1872	T1873	amod	Similar,observations
R1149	T1873	T1874	nsubjpass	observations,made
R115	T277	T266	punct	),utility
R1150	T1875	T1874	aux	have,made
R1151	T1876	T1874	auxpass	been,made
R1152	T1877	T1874	agent	by,made
R1153	T1878	T1877	pobj	Furuta,by
R1154	T1879	T1878	cc	and,Furuta
R1155	T1880	T1878	conj	colleagues,Furuta
R1156	T1881	T1882	advmod	when,used
R1157	T1882	T1874	advcl	used,made
R1158	T1883	T1882	nsubj	they,used
R1159	T1884	T1885	amod	recombinant,virus
R116	T278	T266	prep	in,utility
R1160	T1885	T1882	dobj	virus,used
R1161	T1886	T1885	compound	sindbis,virus
R1162	T1887	T1882	prep	for,used
R1163	T1888	T1889	det	the,labeling
R1164	T1889	T1887	pobj	labeling,for
R1165	T1890	T1889	prep	of,labeling
R1166	T1891	T1890	pobj	dendrites,of
R1167	T1892	T1891	cc	and,dendrites
R1168	T1893	T1891	conj	axons,dendrites
R1169	T1894	T1889	prep	within,labeling
R117	T279	T280	amod	embryonic,stem
R1170	T1895	T1896	det	the,CNS
R1171	T1896	T1894	pobj	CNS,within
R1172	T1897	T1896	prep	of,CNS
R1173	T1898	T1899	amod	adult,mice
R1174	T1899	T1897	pobj	mice,of
R1175	T1900	T1901	punct	[,10
R1176	T1901	T1874	parataxis	10,made
R1177	T1902	T1901	punct	],10
R1178	T1903	T1874	punct	.,made
R1179	T1905	T1906	prep	In,introduced
R118	T280	T281	nmod	stem,cells
R1180	T1907	T1905	pobj	order,In
R1181	T1908	T1909	aux	to,generate
R1182	T1909	T1907	acl	generate,order
R1183	T1910	T1911	det	a,variant
R1184	T1911	T1909	dobj	variant,generate
R1185	T1912	T1911	compound	DsRed,variant
R1186	T1913	T1914	dep	that,be
R1187	T1914	T1911	relcl	be,variant
R1188	T1915	T1914	aux	may,be
R1189	T1916	T1914	acomp	amenable,be
R119	T281	T278	pobj	cells,in
R1190	T1917	T1916	prep	to,amenable
R1191	T1918	T1917	pobj	use,to
R1192	T1919	T1918	prep	in,use
R1193	T1920	T1921	compound	ES,cells
R1194	T1921	T1919	pobj	cells,in
R1195	T1922	T1921	cc	and,cells
R1196	T1923	T1921	conj	mice,cells
R1197	T1924	T1906	punct	", ",introduced
R1198	T1925	T1906	nsubj	we,introduced
R1199	T1926	T1906	advmod	recently,introduced
R12	T163	T158	acl	expressing,cells
R120	T282	T280	punct	(,stem
R1200	T1927	T1928	amod	various,sequences
R1201	T1928	T1906	dobj	sequences,introduced
R1202	T1929	T1930	dep	that,direct
R1203	T1930	T1928	relcl	direct,sequences
R1204	T1931	T1932	amod	nuclear,localization
R1205	T1932	T1930	dobj	localization,direct
R1206	T1933	T1906	prep	to,introduced
R1207	T1934	T1935	det	the,region
R1208	T1935	T1933	pobj	region,to
R1209	T1936	T1935	nmod	DsRed,region
R121	T283	T280	appos	ES,stem
R1210	T1937	T1935	amod	coding,region
R1211	T1938	T1906	punct	.,introduced
R1212	T1940	T1941	advmod	However,were
R1213	T1942	T1941	punct	", ",were
R1214	T1943	T1941	advmod	again,were
R1215	T1944	T1941	nsubj	we,were
R1216	T1945	T1941	acomp	unable,were
R1217	T1946	T1947	aux	to,generate
R1218	T1947	T1945	xcomp	generate,unable
R1219	T1948	T1949	compound	ES,cells
R122	T284	T281	punct	),cells
R1220	T1949	T1947	dobj	cells,generate
R1221	T1950	T1949	cc	or,cells
R1222	T1951	T1949	conj	mice,cells
R1223	T1952	T1949	acl	exhibiting,cells
R1224	T1953	T1954	amod	robust,activity
R1225	T1954	T1952	dobj	activity,exhibiting
R1226	T1955	T1954	amod	widespread,activity
R1227	T1956	T1954	compound	reporter,activity
R1228	T1957	T1958	punct	(,Papaioannou
R1229	T1958	T1941	meta	Papaioannou,were
R123	T285	T281	cc	and,cells
R1230	T1959	T1960	nmod	A.,K.H.
R1231	T1960	T1958	nmod	K.H.,Papaioannou
R1232	T1961	T1960	punct	-,K.H.
R1233	T1962	T1960	cc	and,K.H.
R1234	T1963	T1960	conj	V.E.,K.H.
R1235	T1964	T1958	punct	", ",Papaioannou
R1236	T1965	T1966	amod	unpublished,observations
R1237	T1966	T1958	dep	observations,Papaioannou
R1238	T1967	T1958	punct	),Papaioannou
R1239	T1968	T1941	punct	.,were
R124	T286	T281	conj	mice,cells
R1240	T1971	T1972	advmod	Two,ECFP+
R1241	T1972	T1973	amod	ECFP+,clones
R1242	T1973	T1977	nsubjpass	clones,used
R1243	T1974	T1972	cc	and,ECFP+
R1244	T1975	T1976	advmod	two,EYFP+
R1245	T1976	T1972	conj	EYFP+,ECFP+
R1246	T1978	T1973	acl	exhibiting,clones
R1247	T1979	T1980	amod	robust,fluorescence
R1248	T1980	T1978	dobj	fluorescence,exhibiting
R1249	T1981	T1977	auxpass	were,used
R125	T287	T288	punct	[,2
R1250	T1982	T1983	aux	to,generate
R1251	T1983	T1977	advcl	generate,used
R1252	T1984	T1983	dobj	chimeras,generate
R1253	T1985	T1983	prep	through,generate
R1254	T1986	T1987	compound	ES,cell
R1255	T1987	T1985	pobj	cell,through
R1256	T1988	T1989	punct	<,aggregation
R1257	T1989	T1987	prep	aggregation,cell
R1258	T1990	T1989	punct	-,aggregation
R1259	T1991	T1989	punct	>,aggregation
R126	T288	T262	parataxis	2,demonstrated
R1260	T1992	T1993	compound	morula,stage
R1261	T1993	T1989	compound	stage,aggregation
R1262	T1994	T1989	compound	embryo,aggregation
R1263	T1995	T1996	punct	[,13
R1264	T1996	T1977	parataxis	13,used
R1265	T1997	T1996	punct	],13
R1266	T1998	T1977	punct	.,used
R1267	T2000	T2001	det	All,lines
R1268	T2001	T2005	nsubj	lines,transmitted
R1269	T2002	T2001	nummod	four,lines
R127	T289	T288	punct	],2
R1270	T2003	T2001	compound	ES,lines
R1271	T2004	T2001	compound	cell,lines
R1272	T2006	T2005	prep	through,transmitted
R1273	T2007	T2008	det	the,germline
R1274	T2008	T2006	pobj	germline,through
R1275	T2009	T2005	punct	", ",transmitted
R1276	T2010	T2005	advcl	leading,transmitted
R1277	T2011	T2010	prep	to,leading
R1278	T2012	T2013	det	the,establishment
R1279	T2013	T2011	pobj	establishment,to
R128	T290	T262	punct	.,demonstrated
R1280	T2014	T2013	prep	of,establishment
R1281	T2015	T2014	punct	", ",of
R1282	T2016	T2017	compound	CK4,ECFP
R1283	T2017	T2014	pobj	ECFP,of
R1284	T2018	T2017	punct	/,ECFP
R1285	T2019	T2017	cc	and,ECFP
R1286	T2020	T2021	compound	CK6,ECFP
R1287	T2021	T2017	conj	ECFP,ECFP
R1288	T2022	T2021	punct	/,ECFP
R1289	T2023	T2017	punct	", ",ECFP
R129	T294	T295	prep	In,used
R1290	T2024	T2025	nummod	two,strains
R1291	T2025	T2017	appos	strains,ECFP
R1292	T2026	T2025	prep	of,strains
R1293	T2027	T2026	pobj	mice,of
R1294	T2028	T2025	acl	having,strains
R1295	T2029	T2030	nmod	cyan,protein
R1296	T2030	T2032	compound	protein,expression
R1297	T2031	T2030	amod	fluorescent,protein
R1298	T2032	T2028	dobj	expression,having
R1299	T2033	T2017	cc	and,ECFP
R13	T164	T165	amod	different,variants
R130	T296	T297	det	this,study
R1300	T2034	T2017	punct	", ",ECFP
R1301	T2035	T2036	compound	YC5,EYFP
R1302	T2036	T2017	conj	EYFP,ECFP
R1303	T2037	T2036	punct	/,EYFP
R1304	T2038	T2036	cc	and,EYFP
R1305	T2039	T2040	compound	2A4,EYFP
R1306	T2040	T2036	conj	EYFP,EYFP
R1307	T2041	T2040	punct	/,EYFP
R1308	T2042	T2036	punct	", ",EYFP
R1309	T2043	T2044	nummod	two,strains
R131	T297	T294	pobj	study,In
R1310	T2044	T2036	appos	strains,EYFP
R1311	T2045	T2044	acl	having,strains
R1312	T2046	T2047	amod	widespread,expression
R1313	T2047	T2045	dobj	expression,having
R1314	T2048	T2049	amod	yellow,protein
R1315	T2049	T2047	compound	protein,expression
R1316	T2050	T2049	amod	fluorescent,protein
R1317	T2051	T2005	punct	.,transmitted
R1318	T2053	T2054	prep	In,is
R1319	T2055	T2056	det	these,mice
R132	T298	T295	nsubj	we,used
R1320	T2056	T2053	pobj	mice,In
R1321	T2057	T2058	det	the,expression
R1322	T2058	T2054	nsubj	expression,is
R1323	T2059	T2058	amod	respective,expression
R1324	T2060	T2058	compound	FP,expression
R1325	T2061	T2054	acomp	constituitive,is
R1326	T2062	T2054	punct	", ",is
R1327	T2063	T2054	advcl	starting,is
R1328	T2064	T2063	prep	at,starting
R1329	T2065	T2066	compound	preimplantation,stages
R133	T299	T295	aux	have,used
R1330	T2066	T2064	pobj	stages,at
R1331	T2067	T2063	cc	and,starting
R1332	T2068	T2063	conj	continuing,starting
R1333	T2069	T2068	prep	through,continuing
R1334	T2070	T2071	compound	postimplantation,embryogenesis
R1335	T2071	T2069	pobj	embryogenesis,through
R1336	T2072	T2068	prep	to,continuing
R1337	T2073	T2072	pobj	adulthood,to
R1338	T2074	T2075	punct	(,Fig.
R1339	T2075	T2054	parataxis	Fig.,is
R134	T300	T301	amod	embryonic,stem
R1340	T2076	T2075	nummod	2,Fig.
R1341	T2077	T2075	punct	),Fig.
R1342	T2078	T2054	punct	.,is
R1343	T2080	T2081	nummod	One,line
R1344	T2081	T2082	nsubjpass	line,chosen
R1345	T2083	T2081	acl	expressing,line
R1346	T2084	T2085	det	each,color
R1347	T2085	T2083	dobj	color,expressing
R1348	T2086	T2082	auxpass	was,chosen
R1349	T2087	T2082	prep	for,chosen
R135	T301	T302	nmod	stem,cell
R1350	T2088	T2089	amod	further,analysis
R1351	T2089	T2087	pobj	analysis,for
R1352	T2090	T2089	cc	and,analysis
R1353	T2091	T2089	conj	breeding,analysis
R1354	T2092	T2091	prep	to,breeding
R1355	T2093	T2092	pobj	homozygosity,to
R1356	T2094	T2082	punct	.,chosen
R1357	T2096	T2097	det	The,availability
R1358	T2097	T2098	nsubj	availability,demonstrated
R1359	T2099	T2097	prep	of,availability
R136	T302	T306	npadvmod	cell,mediated
R1360	T2100	T2101	amod	homozygous,animals
R1361	T2101	T2099	pobj	animals,of
R1362	T2102	T2103	mark	that,were
R1363	T2103	T2098	ccomp	were,demonstrated
R1364	T2104	T2105	preconj	both,expression
R1365	T2105	T2103	nsubj	expression,were
R1366	T2106	T2107	compound	reporter,gene
R1367	T2107	T2105	compound	gene,expression
R1368	T2108	T2105	cc	and,expression
R1369	T2109	T2110	det	the,site
R137	T303	T301	punct	(,stem
R1370	T2110	T2105	conj	site,expression
R1371	T2111	T2110	prep	of,site
R1372	T2112	T2113	compound	transgene,integration
R1373	T2113	T2111	pobj	integration,of
R1374	T2114	T2115	advmod	developmentally,neutral
R1375	T2115	T2103	acomp	neutral,were
R1376	T2116	T2098	punct	.,demonstrated
R1377	T2185	T2183	pobj	stages,to
R1378	T2186	T2187	punct	(,2h
R1379	T2187	T2185	parataxis	2h,stages
R138	T304	T301	appos	ES,stem
R1380	T2188	T2187	nmod	Fig.,2h
R1381	T2189	T2187	nmod	2e,2h
R1382	T2190	T2187	punct	",",2h
R1383	T2191	T2187	nmod	2f,2h
R1384	T2192	T2187	punct	",",2h
R1385	T2193	T2187	nmod	2g,2h
R1386	T2194	T2187	punct	",",2h
R1387	T2119	T2120	amod	Zygotic,expression
R1388	T2195	T2187	punct	),2h
R1389	T2120	T2121	nsubj	expression,is
R139	T305	T302	punct	),cell
R1390	T2196	T2153	punct	.,demonstrates
R1391	T2122	T2120	prep	of,expression
R1392	T2123	T2124	det	the,transgenes
R1393	T2198	T2199	advmod	Additionally,discriminated
R1394	T2124	T2122	pobj	transgenes,of
R1395	T2125	T2124	compound	FP,transgenes
R1396	T2126	T2127	advmod	clearly,evident
R1397	T2127	T2121	acomp	evident,is
R1398	T2200	T2201	amod	adult,mice
R1399	T2128	T2121	prep	by,is
R14	T165	T163	dobj	variants,expressing
R140	T306	T308	amod	mediated,transgenesis
R1400	T2129	T2130	det	the,stage
R1401	T2201	T2199	nsubjpass	mice,discriminated
R1402	T2130	T2128	pobj	stage,by
R1403	T2131	T2130	compound	blastocyst,stage
R1404	T2132	T2133	punct	(,2d
R1405	T2202	T2199	aux	can,discriminated
R1406	T2133	T2121	parataxis	2d,is
R1407	T2134	T2133	nmod	Fig.,2d
R1408	T2135	T2133	nmod	2a,2d
R1409	T2203	T2199	advmod	also,discriminated
R141	T307	T306	punct	-,mediated
R1410	T2136	T2133	punct	",",2d
R1411	T2137	T2133	nmod	2b,2d
R1412	T2138	T2133	punct	",",2d
R1413	T2204	T2199	auxpass	be,discriminated
R1414	T2139	T2133	nmod	2c,2d
R1415	T2140	T2133	punct	",",2d
R1416	T2205	T2199	prep	on,discriminated
R1417	T2141	T2133	punct	),2d
R1418	T2142	T2121	cc	and,is
R1419	T2143	T2121	conj	remains,is
R142	T308	T295	dobj	transgenesis,used
R1420	T2206	T2207	det	the,basis
R1421	T2144	T2143	acomp	widespread,remains
R1422	T2145	T2143	advmod	thereafter,remains
R1423	T2207	T2205	pobj	basis,on
R1424	T2146	T2147	punct	(,Fig.
R1425	T2147	T2143	parataxis	Fig.,remains
R1426	T2148	T2147	nummod	2,Fig.
R1427	T2208	T2207	prep	of,basis
R1428	T2149	T2147	punct	),Fig.
R1429	T2209	T2210	det	the,color
R143	T309	T310	aux	to,test
R1430	T2150	T2121	punct	.,is
R1431	T2152	T2153	nsubj	Figure,demonstrates
R1432	T2210	T2208	pobj	color,of
R1433	T2154	T2152	nummod	2,Figure
R1434	T2155	T2156	mark	that,distinguished
R1435	T2156	T2153	ccomp	distinguished,demonstrates
R1436	T2157	T2158	amod	ECFP+,embryos
R1437	T2158	T2156	nsubjpass	embryos,distinguished
R1438	T2159	T2156	aux	can,distinguished
R1439	T2160	T2156	advmod	easily,distinguished
R144	T310	T295	advcl	test,used
R1440	T2161	T2156	auxpass	be,distinguished
R1441	T2211	T2210	prep	of,color
R1442	T2162	T2156	prep	from,distinguished
R1443	T2163	T2164	amod	EYFP+,embryos
R1444	T2164	T2162	pobj	embryos,from
R1445	T2212	T2213	poss	their,fluorescence
R1446	T2165	T2162	cc	and,from
R1447	T2166	T2162	conj	from,from
R1448	T2167	T2168	amod	non-transgenic,embryos
R1449	T2213	T2211	pobj	fluorescence,of
R145	T311	T312	det	the,protein
R1450	T2168	T2166	pobj	embryos,from
R1451	T2169	T2168	amod	non-fluorescent,embryos
R1452	T2214	T2215	punct	(,2k
R1453	T2170	T2168	prep	from,embryos
R1454	T2215	T2199	parataxis	2k,discriminated
R1455	T2171	T2172	compound	preimplantation,stages
R1456	T2172	T2170	pobj	stages,from
R1457	T2216	T2215	nmod	Fig.,2k
R1458	T2173	T2174	punct	(,2d
R1459	T2174	T2172	parataxis	2d,stages
R146	T312	T310	dobj	protein,test
R1460	T2217	T2215	nmod	2i,2k
R1461	T2175	T2174	nmod	Fig.,2d
R1462	T2176	T2174	nmod	2a,2d
R1463	T2177	T2174	punct	",",2d
R1464	T2218	T2215	punct	",",2k
R1465	T2178	T2174	nmod	2b,2d
R1466	T2179	T2174	punct	",",2d
R1467	T2180	T2174	nmod	2c,2d
R1468	T2219	T2215	nmod	2j,2k
R1469	T2181	T2174	punct	",",2d
R147	T313	T312	amod	enhanced,protein
R1470	T2182	T2174	punct	),2d
R1471	T2183	T2168	prep	to,embryos
R1472	T2220	T2215	punct	",",2k
R1473	T2184	T2185	compound	postimplantation,stages
R1474	T2221	T2215	punct	),2k
R1475	T2222	T2199	punct	.,discriminated
R1476	T2224	T2225	advmod	Even,failed
R1477	T2225	T2233	advcl	failed,selected
R1478	T2226	T2225	mark	though,failed
R1479	T2227	T2228	poss	our,lines
R148	T314	T312	nmod	cyan,protein
R1480	T2291	T2292	advmod	Even,lost
R1481	T2228	T2225	nsubj	lines,failed
R1482	T2292	T2296	advcl	lost,went
R1483	T2293	T2292	mark	though,lost
R1484	T2229	T2228	nmod	RFP,lines
R1485	T2294	T2292	nsubj	they,lost
R1486	T2295	T2292	aux	had,lost
R1487	T2297	T2298	poss	their,morphology
R1488	T2230	T2228	amod	transgenic,lines
R1489	T2298	T2292	dobj	morphology,lost
R149	T315	T312	amod	fluorescent,protein
R1490	T2299	T2298	amod	characteristic,morphology
R1491	T2300	T2301	compound	ES,cell
R1492	T2301	T2298	compound	cell,morphology
R1493	T2231	T2232	compound	ES,cell
R1494	T2302	T2303	det	all,lines
R1495	T2303	T2296	nsubj	lines,went
R1496	T2304	T2303	nummod	three,lines
R1497	T2232	T2228	compound	cell,lines
R1498	T2305	T2303	amod	RFP+,lines
R1499	T2306	T2307	compound	ES,cell
R15	T166	T165	compound	GFP,variants
R150	T316	T312	punct	(,protein
R1500	T2234	T2235	aux	to,maintain
R1501	T2307	T2303	compound	cell,lines
R1502	T2308	T2296	advmod	gemline,went
R1503	T2309	T2310	mark	as,detected
R1504	T2310	T2296	advcl	detected,went
R1505	T2235	T2225	xcomp	maintain,failed
R1506	T2311	T2310	prep	by,detected
R1507	T2312	T2313	compound	coat,color
R1508	T2236	T2237	amod	strong,expression
R1509	T2313	T2311	pobj	color,by
R151	T317	T312	appos	ECFP,protein
R1510	T2314	T2313	cc	and,color
R1511	T2315	T2313	conj	PCR,color
R1512	T2237	T2235	dobj	expression,maintain
R1513	T2316	T2315	prep	for,PCR
R1514	T2317	T2318	det	the,transgene
R1515	T2318	T2316	pobj	transgene,for
R1516	T2238	T2237	amod	uniform,expression
R1517	T2319	T2318	compound	DsRed1,transgene
R1518	T2320	T2321	punct	(,shown
R1519	T2321	T2296	parataxis	shown,went
R152	T318	T312	punct	),protein
R1520	T2239	T2235	prep	with,maintain
R1521	T2322	T2321	nsubj	data,shown
R1522	T2323	T2321	neg	not,shown
R1523	T2324	T2321	punct	),shown
R1524	T2240	T2241	advmod	in,vitro
R1525	T2325	T2296	punct	.,went
R1526	T2327	T2328	advmod	However,detected
R1527	T2241	T2242	amod	vitro,passage
R1528	T2329	T2328	nsubjpass	expression,detected
R1529	T2242	T2239	pobj	passage,with
R153	T319	T312	cc	and,protein
R1530	T2330	T2329	prep	of,expression
R1531	T2331	T2332	det	the,reporter
R1532	T2332	T2330	pobj	reporter,of
R1533	T2243	T2233	punct	", ",selected
R1534	T2333	T2332	compound	RFP,reporter
R1535	T2334	T2328	aux	could,detected
R1536	T2335	T2328	neg	not,detected
R1537	T2244	T2233	nsubj	we,selected
R1538	T2336	T2328	auxpass	be,detected
R1539	T2337	T2328	prep	in,detected
R154	T320	T321	amod	enhanced,protein
R1540	T2338	T2339	det	any,organ
R1541	T2339	T2337	pobj	organ,in
R1542	T2340	T2339	prep	of,organ
R1543	T2245	T2246	nummod	three,lines
R1544	T2341	T2342	amod	adult,mice
R1545	T2246	T2233	dobj	lines,selected
R1546	T2342	T2340	pobj	mice,of
R1547	T2343	T2337	cc	nor,in
R1548	T2247	T2246	acl	exhibiting,lines
R1549	T2344	T2337	conj	in,in
R155	T321	T312	conj	protein,protein
R1550	T2345	T2344	pobj	embryos,in
R1551	T2248	T2249	det	the,expression
R1552	T2346	T2347	punct	(,shown
R1553	T2347	T2328	parataxis	shown,detected
R1554	T2249	T2247	dobj	expression,exhibiting
R1555	T2348	T2347	nsubj	data,shown
R1556	T2349	T2347	neg	not,shown
R1557	T2350	T2347	punct	),shown
R1558	T2250	T2251	advmod	most,robust
R1559	T2351	T2328	punct	.,detected
R156	T322	T321	amod	yellow,protein
R1560	T2251	T2249	amod	robust,expression
R1561	T2353	T2354	nsubj	This,suggests
R1562	T2252	T2249	prep	for,expression
R1563	T2355	T2356	mark	that,was
R1564	T2356	T2354	ccomp	was,suggests
R1565	T2253	T2252	pcomp	making,for
R1566	T2357	T2358	det	the,reporter
R1567	T2358	T2356	nsubj	reporter,was
R1568	T2359	T2358	compound	DsRed1,reporter
R1569	T2254	T2253	dobj	chimeras,making
R157	T323	T321	amod	fluorescent,protein
R1570	T2360	T2358	compound	RFP,reporter
R1571	T2361	T2356	acomp	unable,was
R1572	T2362	T2363	aux	to,recapitulate
R1573	T2255	T2233	punct	", ",selected
R1574	T2363	T2361	xcomp	recapitulate,unable
R1575	T2364	T2365	det	the,profile
R1576	T2365	T2363	dobj	profile,recapitulate
R1577	T2256	T2233	prep	in,selected
R1578	T2366	T2365	compound	expression,profile
R1579	T2367	T2365	prep	of,profile
R158	T324	T321	punct	(,protein
R1580	T2368	T2369	det	the,combination
R1581	T2257	T2256	pobj	order,in
R1582	T2369	T2367	pobj	combination,of
R1583	T2370	T2371	compound	promoter,enhancer
R1584	T2371	T2369	compound	enhancer,combination
R1585	T2258	T2259	aux	to,ascertain
R1586	T2372	T2371	punct	/,enhancer
R1587	T2373	T2369	acl	employed,combination
R1588	T2259	T2257	acl	ascertain,order
R1589	T2374	T2354	punct	.,suggests
R159	T325	T321	appos	EYFP,protein
R1590	T2376	T2377	advmod	Therefore,appears
R1591	T2378	T2377	nsubj	it,appears
R1592	T2379	T2380	mark	that,are
R1593	T2380	T2377	ccomp	are,appears
R1594	T2260	T2261	mark	whether,achieve
R1595	T2381	T2382	nmod	GFP,reporters
R1596	T2382	T2380	nsubj	reporters,are
R1597	T2261	T2259	ccomp	achieve,ascertain
R1598	T2383	T2381	amod	variant,GFP
R1599	T2384	T2385	advmod	more,versatile
R16	T167	T163	prep	for,expressing
R160	T326	T312	punct	),protein
R1600	T2385	T2380	acomp	versatile,are
R1601	T2262	T2261	nsubj	we,achieve
R1602	T2386	T2380	prep	with,are
R1603	T2387	T2386	pobj	respect,with
R1604	T2388	T2387	prep	to,respect
R1605	T2389	T2390	poss	their,application
R1606	T2263	T2261	aux	could,achieve
R1607	T2390	T2388	pobj	application,to
R1608	T2391	T2390	prep	in,application
R1609	T2392	T2391	pobj	mice,in
R161	T327	T312	punct	", ",protein
R1610	T2264	T2261	punct	", ",achieve
R1611	T2393	T2377	punct	.,appears
R1612	T2265	T2261	cc	or,achieve
R1613	T2395	T2396	nsubj	We,produced
R1614	T2266	T2267	advmod	possibly,recover
R1615	T2267	T2261	conj	recover,achieve
R1616	T2268	T2261	punct	", ",achieve
R1617	T2397	T2398	amod	double,chimeras
R1618	T2269	T2270	compound	RFP,expression
R1619	T2398	T2396	dobj	chimeras,produced
R162	T328	T329	nummod	two,variants
R1620	T2399	T2400	npadvmod	FP,tagged
R1621	T2400	T2398	amod	tagged,chimeras
R1622	T2270	T2261	dobj	expression,achieve
R1623	T2401	T2400	punct	-,tagged
R1624	T2402	T2396	prep	in,produced
R1625	T2403	T2402	pobj	order,in
R1626	T2271	T2261	prep	in,achieve
R1627	T2404	T2405	aux	to,assess
R1628	T2405	T2403	acl	assess,order
R1629	T2272	T2271	pobj	animals,in
R163	T329	T312	appos	variants,protein
R1630	T2406	T2407	mark	whether,co-visualized
R1631	T2407	T2405	ccomp	co-visualized,assess
R1632	T2408	T2409	det	the,reporters
R1633	T2409	T2407	nsubjpass	reporters,co-visualized
R1634	T2273	T2233	punct	.,selected
R1635	T2410	T2409	nmod	ECFP,reporters
R1636	T2411	T2410	cc	and,ECFP
R1637	T2275	T2276	nsubjpass	Cells,sorted
R1638	T2412	T2410	conj	EYFP,ECFP
R1639	T2413	T2407	aux	could,co-visualized
R164	T330	T329	amod	mutant,variants
R1640	T2414	T2407	auxpass	be,co-visualized
R1641	T2277	T2276	auxpass	were,sorted
R1642	T2415	T2407	prep	within,co-visualized
R1643	T2416	T2417	det	the,animal
R1644	T2278	T2276	dep	flow,sorted
R1645	T2417	T2415	pobj	animal,within
R1646	T2418	T2417	amod	same,animal
R1647	T2419	T2396	punct	.,produced
R1648	T2279	T2276	advmod	prior,sorted
R1649	T2421	T2422	det	The,series
R165	T331	T330	cc	and,mutant
R1650	T2280	T2279	prep	to,prior
R1651	T2422	T2424	nsubjpass	series,generated
R1652	T2423	T2422	amod	first,series
R1653	T2281	T2280	pobj	aggregation,to
R1654	T2425	T2422	prep	of,series
R1655	T2426	T2425	pobj	chimeras,of
R1656	T2282	T2283	advmod	so,enrich
R1657	T2427	T2424	auxpass	were,generated
R1658	T2428	T2424	prep	through,generated
R1659	T2429	T2430	det	the,aggregation
R166	T332	T333	advmod	spectrally,distinct
R1660	T2430	T2428	pobj	aggregation,through
R1661	T2283	T2276	advcl	enrich,sorted
R1662	T2284	T2283	mark	as,enrich
R1663	T2431	T2430	prep	of,aggregation
R1664	T2432	T2433	amod	tetraploid,embryos
R1665	T2285	T2283	aux	to,enrich
R1666	T2433	T2431	pobj	embryos,of
R1667	T2434	T2433	prep	with,embryos
R1668	T2286	T2283	prep	for,enrich
R1669	T2435	T2436	amod	FP+,cells
R167	T333	T330	conj	distinct,mutant
R1670	T2436	T2434	pobj	cells,with
R1671	T2437	T2436	compound	ES,cells
R1672	T2287	T2288	compound	RFP,expression
R1673	T2438	T2436	punct	(,cells
R1674	T2439	T2436	cc	or,cells
R1675	T2440	T2436	conj	embryos,cells
R1676	T2288	T2286	pobj	expression,for
R1677	T2441	T2436	punct	),cells
R1678	T2442	T2436	prep	of,cells
R1679	T2443	T2444	det	the,color
R168	T334	T329	compound	color,variants
R1680	T2289	T2276	punct	.,sorted
R1681	T2444	T2442	pobj	color,of
R1682	T2445	T2444	amod	complementary,color
R1683	T2446	T2424	punct	.,generated
R1684	T2503	T2494	punct	",",3f
R1685	T2448	T2449	det	The,procedure
R1686	T2449	T2450	nsubjpass	procedure,schematized
R1687	T2504	T2494	punct	),3f
R1688	T2451	T2450	auxpass	is,schematized
R1689	T2452	T2450	prep	in,schematized
R169	T335	T329	prep	of,variants
R1690	T2453	T2454	compound	Fig.,3a
R1691	T2454	T2452	pobj	3a,in
R1692	T2505	T2458	punct	.,is
R1693	T2455	T2450	punct	.,schematized
R1694	T2507	T2508	advmod	Secondly,investigated
R1695	T2457	T2458	nsubj	It,is
R1696	T2459	T2458	acomp	evident,is
R1697	T2460	T2461	mark	that,discerned
R1698	T2461	T2458	ccomp	discerned,is
R1699	T2462	T2463	det	the,expression
R17	T168	T169	amod	multiple,usage
R170	T336	T337	amod	wild,type
R1700	T2463	T2461	nsubjpass	expression,discerned
R1701	T2509	T2508	nsubj	we,investigated
R1702	T2464	T2463	prep	of,expression
R1703	T2465	T2466	det	the,FPs
R1704	T2466	T2464	pobj	FPs,of
R1705	T2467	T2466	nummod	two,FPs
R1706	T2510	T2511	compound	reporter,co-visualization
R1707	T2468	T2461	aux	can,discerned
R1708	T2469	T2461	advmod	easily,discerned
R1709	T2511	T2508	dobj	co-visualization,investigated
R171	T337	T338	nmod	type,GFP
R1710	T2470	T2461	auxpass	be,discerned
R1711	T2471	T2461	prep	in,discerned
R1712	T2472	T2473	det	these,embryos
R1713	T2512	T2508	prep	in,investigated
R1714	T2473	T2471	pobj	embryos,in
R1715	T2474	T2475	amod	double,transgenic
R1716	T2475	T2473	amod	transgenic,embryos
R1717	T2513	T2514	amod	balanced,chimeras
R1718	T2476	T2477	advmod	where,is
R1719	T2477	T2473	relcl	is,embryos
R172	T338	T335	pobj	GFP,of
R1720	T2478	T2479	det	the,compartment
R1721	T2514	T2512	pobj	chimeras,in
R1722	T2479	T2477	nsubj	compartment,is
R1723	T2480	T2479	amod	diploid,compartment
R1724	T2481	T2479	punct	", ",compartment
R1725	T2515	T2514	amod	adult,chimeras
R1726	T2482	T2483	npadvmod	embryo,proper
R1727	T2483	T2479	amod	proper,compartment
R1728	T2484	T2483	punct	-,proper
R1729	T2516	T2514	acl	comprised,chimeras
R173	T339	T337	punct	(,type
R1730	T2485	T2477	acomp	ECFP+,is
R1731	T2486	T2477	cc	and,is
R1732	T2487	T2488	det	the,compartment
R1733	T2517	T2516	prep	of,comprised
R1734	T2488	T2491	nsubj	compartment,is
R1735	T2489	T2488	amod	tetraploid,compartment
R1736	T2518	T2519	preconj	both,compartments
R1737	T2490	T2488	amod	extraembryonic,compartment
R1738	T2491	T2477	conj	is,is
R1739	T2492	T2491	acomp	EYFP+,is
R174	T340	T337	appos	wt,type
R1740	T2519	T2517	pobj	compartments,of
R1741	T2493	T2494	punct	(,3f
R1742	T2494	T2458	parataxis	3f,is
R1743	T2495	T2494	nmod	Fig.,3f
R1744	T2496	T2494	nmod	3b,3f
R1745	T2520	T2519	amod	ECFP+,compartments
R1746	T2497	T2494	punct	",",3f
R1747	T2498	T2494	nmod	3c,3f
R1748	T2499	T2494	punct	",",3f
R1749	T2500	T2494	nmod	3d,3f
R175	T341	T338	punct	),GFP
R1750	T2501	T2494	punct	",",3f
R1751	T2502	T2494	nmod	3e,3f
R1752	T2521	T2520	cc	and,ECFP+
R1753	T2522	T2520	conj	EYFP+,ECFP+
R1754	T2523	T2508	punct	.,investigated
R1755	T2609	T2603	auxpass	be,used
R1756	T2525	T2526	nsubjpass	These,generated
R1757	T2610	T2603	prep	in,used
R1758	T2611	T2612	det	the,construction
R1759	T2612	T2610	pobj	construction,in
R176	T342	T295	punct	.,used
R1760	T2613	T2612	prep	of,construction
R1761	T2527	T2526	auxpass	were,generated
R1762	T2614	T2613	pobj	chimeras,of
R1763	T2615	T2616	advmod	so,tag
R1764	T2616	T2603	advcl	tag,used
R1765	T2528	T2526	prep	through,generated
R1766	T2617	T2616	mark	as,tag
R1767	T2618	T2616	aux	to,tag
R1768	T2529	T2530	det	the,aggregation
R1769	T2619	T2620	preconj	both,lineages
R177	T344	T345	nsubj	We,tested
R1770	T2620	T2616	dobj	lineages,tag
R1771	T2621	T2620	amod	mutant,lineages
R1772	T2530	T2528	pobj	aggregation,through
R1773	T2531	T2530	prep	of,aggregation
R1774	T2622	T2621	cc	and,mutant
R1775	T2532	T2533	amod	diploid,embryos
R1776	T2623	T2624	amod	wild,type
R1777	T2624	T2621	conj	type,mutant
R1778	T2533	T2531	pobj	embryos,of
R1779	T2625	T2598	punct	.,envisaged
R178	T346	T345	aux	have,tested
R1780	T2534	T2530	prep	with,aggregation
R1781	T2535	T2536	amod	diploid,embryos
R1782	T2536	T2534	pobj	embryos,with
R1783	T2537	T2536	punct	(,embryos
R1784	T2538	T2536	cc	or,embryos
R1785	T2539	T2540	compound	ES,cells
R1786	T2540	T2536	conj	cells,embryos
R1787	T2541	T2536	punct	),embryos
R1788	T2542	T2536	prep	of,embryos
R1789	T2543	T2544	det	the,color
R179	T347	T345	advmod	also,tested
R1790	T2544	T2542	pobj	color,of
R1791	T2545	T2544	amod	complementary,color
R1792	T2546	T2526	punct	.,generated
R1793	T2548	T2549	det	The,components
R1794	T2549	T2551	nsubjpass	components,schematized
R1795	T2550	T2549	amod	aggregated,components
R1796	T2552	T2549	prep	of,components
R1797	T2553	T2554	det	the,chimera
R1798	T2554	T2552	pobj	chimera,of
R1799	T2555	T2556	prep	from,originated
R18	T169	T167	pobj	usage,for
R180	T348	T345	dobj	DsRed1,tested
R1800	T2556	T2554	relcl	originated,chimera
R1801	T2557	T2555	pobj	which,from
R1802	T2558	T2559	det	each,organ
R1803	T2559	T2556	nsubj	organ,originated
R1804	T2560	T2551	auxpass	is,schematized
R1805	T2561	T2551	prep	in,schematized
R1806	T2562	T2563	det	the,right
R1807	T2563	T2561	pobj	right,in
R1808	T2564	T2563	amod	top,right
R1809	T2565	T2563	prep	of,right
R181	T349	T348	punct	", ",DsRed1
R1810	T2566	T2567	det	each,panel
R1811	T2567	T2565	pobj	panel,of
R1812	T2568	T2567	prep	in,panel
R1813	T2569	T2568	pobj	Fig.,in
R1814	T2570	T2569	nummod	4,Fig.
R1815	T2571	T2551	punct	.,schematized
R1816	T2573	T2574	det	These,organs
R1817	T2574	T2577	nsubj	organs,demonstrate
R1818	T2575	T2574	amod	chimeric,organs
R1819	T2576	T2574	amod	adult,organs
R182	T350	T351	det	the,reporter
R1820	T2578	T2579	mark	that,discerned
R1821	T2579	T2577	ccomp	discerned,demonstrate
R1822	T2580	T2581	det	the,cells
R1823	T2581	T2579	nsubjpass	cells,discerned
R1824	T2582	T2581	amod	ECFP+,cells
R1825	T2583	T2582	cc	and,ECFP+
R1826	T2584	T2582	conj	EYFP+,ECFP+
R1827	T2585	T2581	acl	comprising,cells
R1828	T2586	T2587	det	these,chimeras
R1829	T2587	T2585	dobj	chimeras,comprising
R183	T351	T348	appos	reporter,DsRed1
R1830	T2588	T2579	aux	can,discerned
R1831	T2589	T2579	advmod	easily,discerned
R1832	T2590	T2579	auxpass	be,discerned
R1833	T2591	T2592	punct	(,Fig.
R1834	T2592	T2577	parataxis	Fig.,demonstrate
R1835	T2593	T2592	nummod	4,Fig.
R1836	T2594	T2592	punct	),Fig.
R1837	T2595	T2577	punct	.,demonstrate
R1838	T2597	T2598	nsubjpass	It,envisaged
R1839	T2599	T2598	aux	can,envisaged
R184	T352	T351	amod	novel,reporter
R1840	T2600	T2598	advmod	therefore,envisaged
R1841	T2601	T2598	auxpass	be,envisaged
R1842	T2602	T2603	mark	that,used
R1843	T2603	T2598	ccomp	used,envisaged
R1844	T2604	T2605	nummod	two,colors
R1845	T2605	T2603	nsubjpass	colors,used
R1846	T2606	T2605	amod	different,colors
R1847	T2607	T2605	compound	FP,colors
R1848	T2608	T2603	aux	could,used
R1849	T2695	T2696	prep	From,conclude
R185	T353	T354	amod	red,protein
R1850	T2697	T2698	det	these,observations
R1851	T2698	T2695	pobj	observations,From
R1852	T2699	T2696	nsubj	we,conclude
R1853	T2700	T2701	mark	that,are
R1854	T2701	T2696	ccomp	are,conclude
R1855	T2702	T2703	det	the,ECFP
R1856	T2703	T2701	nsubj	ECFP,are
R1857	T2704	T2705	amod	blue,shifted
R1858	T2705	T2703	amod	shifted,ECFP
R1859	T2706	T2705	punct	-,shifted
R186	T354	T351	compound	protein,reporter
R1860	T2707	T2703	punct	", ",ECFP
R1861	T2708	T2703	cc	and,ECFP
R1862	T2709	T2710	det	the,EYFP
R1863	T2710	T2714	compound	EYFP,variants
R1864	T2711	T2712	amod	red,shifted
R1865	T2712	T2710	amod	shifted,EYFP
R1866	T2713	T2712	punct	-,shifted
R1867	T2714	T2703	conj	variants,ECFP
R1868	T2715	T2703	prep	of,ECFP
R1869	T2716	T2715	pobj	wtGFP,of
R187	T355	T354	amod	fluorescent,protein
R1870	T2717	T2701	advmod	both,are
R1871	T2718	T2701	acomp	amenable,are
R1872	T2719	T2718	prep	to,amenable
R1873	T2720	T2719	pobj	use,to
R1874	T2721	T2720	prep	in,use
R1875	T2722	T2721	pobj	mice,in
R1876	T2723	T2696	punct	.,conclude
R1877	T2725	T2726	advmod	Also,demonstrated
R1878	T2727	T2728	mark	since,established
R1879	T2728	T2726	advcl	established,demonstrated
R188	T356	T357	advmod	recently,cloned
R1880	T2729	T2728	nsubj	we,established
R1881	T2730	T2728	aux	have,established
R1882	T2731	T2728	dobj	lines,established
R1883	T2732	T2731	prep	of,lines
R1884	T2733	T2734	amod	viable,mice
R1885	T2734	T2732	pobj	mice,of
R1886	T2735	T2733	cc	and,viable
R1887	T2736	T2733	conj	fertile,viable
R1888	T2737	T2731	acl	having,lines
R1889	T2738	T2739	amod	widespread,expression
R189	T357	T351	acl	cloned,reporter
R1890	T2739	T2737	dobj	expression,having
R1891	T2740	T2739	compound	FP,expression
R1892	T2741	T2726	nsubj	we,demonstrated
R1893	T2742	T2726	aux	have,demonstrated
R1894	T2743	T2744	mark	that,represent
R1895	T2744	T2726	ccomp	represent,demonstrated
R1896	T2745	T2744	nsubj	ECFP,represent
R1897	T2746	T2745	cc	and,ECFP
R1898	T2747	T2745	conj	EYFP,ECFP
R1899	T2748	T2749	advmod	developmentally,neutral
R19	T170	T169	amod	non-invasive,usage
R190	T358	T357	prep	from,cloned
R1900	T2749	T2750	amod	neutral,reporters
R1901	T2750	T2744	dobj	reporters,represent
R1902	T2751	T2726	punct	.,demonstrated
R1903	T2753	T2754	mark	Since,were
R1904	T2754	T2756	advcl	were,conclude
R1905	T2755	T2754	nsubj	we,were
R1906	T2757	T2754	acomp	unable,were
R1907	T2758	T2759	aux	to,obtain
R1908	T2759	T2757	xcomp	obtain,unable
R1909	T2760	T2761	compound	ES,cells
R191	T359	T360	det	the,coral
R1910	T2761	T2759	dobj	cells,obtain
R1911	T2762	T2761	punct	", ",cells
R1912	T2763	T2761	conj	embryos,cells
R1913	T2764	T2763	cc	or,embryos
R1914	T2765	T2763	conj	mice,embryos
R1915	T2766	T2761	prep	with,cells
R1916	T2767	T2768	amod	widespread,expression
R1917	T2768	T2766	pobj	expression,with
R1918	T2769	T2768	prep	of,expression
R1919	T2770	T2771	det	a,RFP
R192	T360	T358	pobj	coral,from
R1920	T2771	T2769	pobj	RFP,of
R1921	T2772	T2771	punct	(,RFP
R1922	T2773	T2771	appos	DsRed1,RFP
R1923	T2774	T2756	punct	),conclude
R1924	T2775	T2756	nsubj	we,conclude
R1925	T2776	T2777	mark	that,is
R1926	T2777	T2756	advcl	is,conclude
R1927	T2778	T2779	det	this,reporter
R1928	T2779	T2777	nsubj	reporter,is
R1929	T2780	T2777	neg	not,is
R193	T361	T360	compound	Discostoma,coral
R1930	T2781	T2782	advmod	developmentally,neutral
R1931	T2782	T2777	acomp	neutral,is
R1932	T2783	T2777	cc	or,is
R1933	T2784	T2785	mark	that,sustained
R1934	T2785	T2777	conj	sustained,is
R1935	T2786	T2787	poss	its,expression
R1936	T2787	T2785	nsubjpass	expression,sustained
R1937	T2788	T2785	aux	can,sustained
R1938	T2789	T2785	neg	not,sustained
R1939	T2790	T2785	auxpass	be,sustained
R194	T362	T357	prep	by,cloned
R1940	T2791	T2785	prep	at,sustained
R1941	T2792	T2791	pobj	levels,at
R1942	T2793	T2794	amod	high,enough
R1943	T2794	T2792	amod	enough,levels
R1944	T2795	T2794	prep	for,enough
R1945	T2796	T2795	pobj	detection,for
R1946	T2797	T2785	punct	", ",sustained
R1947	T2798	T2785	cc	and,sustained
R1948	T2799	T2800	prep	as,is
R1949	T2800	T2785	conj	is,sustained
R195	T363	T362	pobj	virtue,by
R1950	T2801	T2799	amod	such,as
R1951	T2802	T2800	neg	not,is
R1952	T2803	T2804	advmod	as,versatile
R1953	T2804	T2800	acomp	versatile,is
R1954	T2805	T2804	prep	as,versatile
R1955	T2806	T2807	amod	other,reporters
R1956	T2807	T2805	pobj	reporters,as
R1957	T2808	T2809	npadvmod	gene,based
R1958	T2809	T2807	amod	based,reporters
R1959	T2810	T2809	punct	-,based
R196	T364	T363	prep	of,virtue
R1960	T2811	T2807	amod	fluorescent,reporters
R1961	T2812	T2756	punct	.,conclude
R1962	T2814	T2815	nsubjpass	ECFP,distinguished
R1963	T2816	T2814	cc	and,ECFP
R1964	T2817	T2814	conj	EYFP,ECFP
R1965	T2818	T2815	aux	can,distinguished
R1966	T2819	T2815	auxpass	be,distinguished
R1967	T2820	T2815	prep	on,distinguished
R1968	T2821	T2822	det	the,basis
R1969	T2822	T2820	pobj	basis,on
R197	T365	T366	poss	its,homology
R1970	T2823	T2822	prep	of,basis
R1971	T2824	T2825	poss	their,profiles
R1972	T2825	T2823	pobj	profiles,of
R1973	T2826	T2825	amod	unique,profiles
R1974	T2827	T2825	amod	non-overlapping,profiles
R1975	T2828	T2825	amod	spectral,profiles
R1976	T2829	T2815	punct	", ",distinguished
R1977	T2830	T2815	cc	and,distinguished
R1978	T2831	T2832	nsubj	chimeras,demonstrate
R1979	T2832	T2815	conj	demonstrate,distinguished
R198	T366	T364	pobj	homology,of
R1980	T2833	T2831	acl	comprised,chimeras
R1981	T2834	T2833	prep	of,comprised
R1982	T2835	T2836	npadvmod	ECFP,tagged
R1983	T2836	T2839	amod	tagged,compartments
R1984	T2837	T2835	cc	and,ECFP
R1985	T2838	T2835	conj	EYFP,ECFP
R1986	T2839	T2834	pobj	compartments,of
R1987	T2840	T2841	mark	that,visualized
R1988	T2841	T2832	ccomp	visualized,demonstrate
R1989	T2842	T2843	det	the,reporters
R199	T367	T366	prep	to,homology
R1990	T2843	T2841	nsubjpass	reporters,visualized
R1991	T2844	T2843	nummod	two,reporters
R1992	T2845	T2841	aux	can,visualized
R1993	T2846	T2841	auxpass	be,visualized
R1994	T2847	T2841	advmod	simultaneously,visualized
R1995	T2848	T2841	advmod	non-invasively,visualized
R1996	T2849	T2841	prep	within,visualized
R1997	T2850	T2851	det	the,embryo
R1998	T2851	T2849	pobj	embryo,within
R1999	T2852	T2851	amod	same,embryo
R20	T171	T169	compound	reporter,usage
R200	T368	T367	pobj	GFP,to
R2000	T2853	T2851	cc	or,embryo
R2001	T2854	T2855	amod	adult,organ
R2002	T2855	T2851	conj	organ,embryo
R2003	T2856	T2832	punct	.,demonstrate
R2004	T2858	T2859	advmod	Therefore,be
R2005	T2860	T2859	nsubj	it,be
R2006	T2861	T2859	aux	should,be
R2007	T2862	T2859	acomp	possible,be
R2008	T2863	T2864	aux	to,use
R2009	T2864	T2859	xcomp	use,be
R201	T369	T345	punct	.,tested
R2010	T2865	T2866	compound	flow,cytometry
R2011	T2866	T2864	dobj	cytometry,use
R2012	T2867	T2868	aux	to,isolate
R2013	T2868	T2864	advcl	isolate,use
R2014	T2869	T2868	dobj	cells,isolate
R2015	T2870	T2869	acl	expressing,cells
R2016	T2871	T2870	dobj	either,expressing
R2017	T2872	T2871	cc	or,either
R2018	T2873	T2871	conj	both,either
R2019	T2874	T2871	prep	of,either
R202	T371	T372	prep	To,established
R2020	T2875	T2876	det	the,reporters
R2021	T2876	T2874	pobj	reporters,of
R2022	T2877	T2868	prep	from,isolate
R2023	T2878	T2877	pobj	embryos,from
R2024	T2879	T2878	cc	or,embryos
R2025	T2880	T2881	amod	adult,organs
R2026	T2881	T2878	conj	organs,embryos
R2027	T2882	T2883	punct	[,14
R2028	T2883	T2859	parataxis	14,be
R2029	T2884	T2883	punct	],14
R203	T373	T374	det	this,end
R2030	T2885	T2859	punct	.,be
R2031	T2887	T2888	det	These,reporters
R2032	T2888	T2890	nsubjpass	reporters,used
R2033	T2889	T2888	nummod	two,reporters
R2034	T2891	T2890	aux	can,used
R2035	T2892	T2890	advmod	also,used
R2036	T2893	T2890	auxpass	be,used
R2037	T2894	T2895	aux	to,create
R2038	T2895	T2890	advcl	create,used
R2039	T2896	T2895	dobj	pairs,create
R204	T374	T371	pobj	end,To
R2040	T2897	T2896	prep	of,pairs
R2041	T2898	T2897	pobj	donors,of
R2042	T2899	T2898	cc	and,donors
R2043	T2900	T2898	conj	acceptors,donors
R2044	T2901	T2895	prep	for,create
R2045	T2902	T2903	advmod	in,vivo
R2046	T2903	T2904	amod	vivo,transfer
R2047	T2904	T2901	pobj	transfer,for
R2048	T2905	T2904	compound	fluorescence,transfer
R2049	T2906	T2904	compound	energy,transfer
R205	T375	T372	punct	", ",established
R2050	T2907	T2904	punct	(,transfer
R2051	T2908	T2904	appos	FRET,transfer
R2052	T2909	T2890	punct	),used
R2053	T2910	T2911	punct	[,15
R2054	T2911	T2890	parataxis	15,used
R2055	T2912	T2911	punct	],15
R2056	T2913	T2890	punct	.,used
R2057	T2915	T2916	mark	Since,are
R2058	T2916	T2924	advcl	are,represent
R2059	T2917	T2918	det	the,profiles
R206	T376	T372	nsubj	we,established
R2060	T2918	T2916	nsubj	profiles,are
R2061	T2919	T2918	compound	emission,profiles
R2062	T2920	T2918	prep	of,profiles
R2063	T2921	T2920	pobj	ECFP,of
R2064	T2922	T2921	cc	and,ECFP
R2065	T2923	T2921	conj	EYFP,ECFP
R2066	T2925	T2926	det	the,overlapping
R2067	T2926	T2916	attr	overlapping,are
R2068	T2927	T2926	advmod	least,overlapping
R2069	T2928	T2926	prep	of,overlapping
R207	T377	T372	aux	have,established
R2070	T2929	T2930	predet	all,variants
R2071	T2930	T2928	pobj	variants,of
R2072	T2931	T2930	det	the,variants
R2073	T2932	T2930	amod	usable,variants
R2074	T2933	T2930	compound	GFP,variants
R2075	T2934	T2924	punct	", ",represent
R2076	T2935	T2936	det	these,ECFP
R2077	T2936	T2924	nsubj	ECFP,represent
R2078	T2937	T2936	cc	and,ECFP
R2079	T2938	T2936	conj	EYFP,ECFP
R208	T378	T372	dobj	lines,established
R2080	T2939	T2924	advmod	currently,represent
R2081	T2940	T2941	det	the,combination
R2082	T2941	T2924	dobj	combination,represent
R2083	T2942	T2941	amod	best,combination
R2084	T2943	T2941	prep	for,combination
R2085	T2944	T2943	pobj	co-visualization,for
R2086	T2945	T2943	cc	and,for
R2087	T2946	T2943	conj	for,for
R2088	T2947	T2946	pobj	use,for
R2089	T2948	T2947	prep	in,use
R209	T379	T378	prep	of,lines
R2090	T2949	T2950	amod	multi-spectral,experiments
R2091	T2950	T2948	pobj	experiments,in
R2092	T2951	T2952	amod	double,tagged
R2093	T2952	T2950	amod	tagged,experiments
R2094	T2953	T2952	punct	-,tagged
R2095	T2954	T2950	amod	transgenic,experiments
R2096	T2955	T2954	punct	", ",transgenic
R2097	T2956	T2954	conj	chimeric,transgenic
R2098	T2957	T2956	cc	and,chimeric
R2099	T2958	T2957	punct	/,and
R21	T172	T163	prep	within,expressing
R210	T380	T381	compound	ES,cells
R2100	T2959	T2957	cc	or,and
R2101	T2960	T2961	advmod	in,vivo
R2102	T2961	T2956	conj	vivo,chimeric
R2103	T2962	T2950	compound	FRET,experiments
R2104	T2963	T2964	punct	(,reviewed
R2105	T2964	T2924	parataxis	reviewed,represent
R2106	T2965	T2964	prep	in,reviewed
R2107	T2966	T2965	punct	[,in
R2108	T2967	T2965	pobj	16,in
R2109	T2968	T2965	punct	],in
R211	T381	T379	pobj	cells,of
R2110	T2969	T2964	punct	),reviewed
R2111	T2970	T2924	punct	.,represent
R2112	T2972	T2973	det	These,experiments
R2113	T2973	T2974	nsubj	experiments,demonstrated
R2114	T2975	T2974	aux	have,demonstrated
R2115	T2976	T2977	det	the,feasibility
R2116	T2977	T2974	dobj	feasibility,demonstrated
R2117	T2978	T2977	prep	of,feasibility
R2118	T2979	T2978	pcomp	monitoring,of
R2119	T2980	T2981	amod	multiple,reporters
R212	T382	T383	advmod	together,with
R2120	T2981	T2979	dobj	reporters,monitoring
R2121	T2982	T2981	compound	FP,reporters
R2122	T2983	T2979	advmod	non-invasively,monitoring
R2123	T2984	T2979	prep	within,monitoring
R2124	T2985	T2986	det	a,animal
R2125	T2986	T2984	pobj	animal,within
R2126	T2987	T2986	amod	single,animal
R2127	T2988	T2974	punct	", ",demonstrated
R2128	T2989	T2974	cc	and,demonstrated
R2129	T2990	T2991	aux	have,shown
R213	T383	T372	prep	with,established
R2130	T2991	T2974	conj	shown,demonstrated
R2131	T2992	T2993	det	the,neutrality
R2132	T2993	T2991	dobj	neutrality,shown
R2133	T2994	T2993	amod	developmental,neutrality
R2134	T2995	T2993	prep	of,neutrality
R2135	T2996	T2997	det	the,reporters
R2136	T2997	T2995	pobj	reporters,of
R2137	T2998	T2997	compound	FP,reporters
R2138	T2999	T2997	acl	employed,reporters
R2139	T3000	T2974	punct	.,demonstrated
R214	T384	T385	amod	viable,mice
R2140	T3002	T3003	nsubj	This,pave
R2141	T3004	T3003	aux	should,pave
R2142	T3005	T3006	det	the,way
R2143	T3006	T3003	dobj	way,pave
R2144	T3007	T3003	prep	for,pave
R2145	T3008	T3009	amod	novel,strategies
R2146	T3009	T3007	pobj	strategies,for
R2147	T3010	T3009	acl	designed,strategies
R2148	T3011	T3012	aux	to,incorporate
R2149	T3012	T3010	advcl	incorporate,designed
R215	T385	T383	pobj	mice,with
R2150	T3013	T3014	det	the,use
R2151	T3014	T3012	dobj	use,incorporate
R2152	T3015	T3014	prep	of,use
R2153	T3016	T3017	compound	compound,transgenics
R2154	T3017	T3015	pobj	transgenics,of
R2155	T3018	T3017	cc	or,transgenics
R2156	T3019	T3020	compound	tissue,recombination
R2157	T3020	T3017	conj	recombination,transgenics
R2158	T3021	T3020	cc	or,recombination
R2159	T3022	T3023	amod	chimeric,analyses
R216	T386	T384	cc	and,viable
R2160	T3023	T3020	conj	analyses,recombination
R2161	T3024	T3025	advmod	where,are
R2162	T3025	T3014	relcl	are,use
R2163	T3026	T3027	amod	several,compartments
R2164	T3027	T3025	nsubj	compartments,are
R2165	T3028	T3029	aux	to,tagged
R2166	T3029	T3025	xcomp	tagged,are
R2167	T3030	T3029	auxpass	be,tagged
R2168	T3031	T3003	punct	.,pave
R2169	T3033	T3034	det	This,technology
R217	T387	T384	conj	fertile,viable
R2170	T3034	T3035	nsubj	technology,provide
R2171	T3036	T3035	aux	should,provide
R2172	T3037	T3038	amod	new,possibilities
R2173	T3038	T3035	dobj	possibilities,provide
R2174	T3039	T3038	prep	in,possibilities
R2175	T3040	T3039	pcomp	manipulating,in
R2176	T3041	T3042	det	the,genome
R2177	T3042	T3040	dobj	genome,manipulating
R2178	T3043	T3042	compound	mouse,genome
R2179	T3044	T3040	punct	", ",manipulating
R218	T388	T385	acl	having,mice
R2180	T3045	T3040	cc	and,manipulating
R2181	T3046	T3047	advmod	non-invasively,studying
R2182	T3047	T3040	conj	studying,manipulating
R2183	T3048	T3049	det	the,consequences
R2184	T3049	T3047	dobj	consequences,studying
R2185	T3050	T3035	punct	.,provide
R2188	T3227	T3228	compound	Vector,construction
R2189	T3230	T3231	nsubjpass	cDNAs,using
R219	T389	T390	amod	widespread,expression
R2190	T3232	T3230	acl	encoding,cDNAs
R2191	T3233	T3232	dobj	ECFP,encoding
R2192	T3234	T3233	punct	", ",ECFP
R2193	T3235	T3233	conj	EYFP,ECFP
R2194	T3236	T3235	cc	and,EYFP
R2195	T3237	T3235	conj	DsRed,EYFP
R2196	T3238	T3231	auxpass	were,using
R2197	T3239	T3231	aux	amplified,using
R2198	T3240	T3231	prep	from,using
R2199	T3241	T3242	compound	plasmids,pECFP
R22	T173	T174	det	a,animal
R220	T390	T388	dobj	expression,having
R2200	T3242	T3240	pobj	pECFP,from
R2201	T3243	T3242	punct	", ",pECFP
R2202	T3244	T3242	conj	pEYFP,pECFP
R2203	T3245	T3244	cc	and,pEYFP
R2204	T3246	T3247	compound	pDsRed1,N1
R2205	T3247	T3244	conj	N1,pEYFP
R2206	T3248	T3247	punct	-,N1
R2207	T3249	T3250	punct	(,Inc.
R2208	T3250	T3242	parataxis	Inc.,pECFP
R2209	T3251	T3250	nmod	Clontech,Inc.
R221	T391	T390	prep	of,expression
R2210	T3252	T3250	punct	", ",Inc.
R2211	T3253	T3250	punct	),Inc.
R2212	T3254	T3231	prep	by,using
R2213	T3255	T3254	pobj	PCR,by
R2214	T3256	T3257	compound	HiFi,Polymerase
R2215	T3257	T3231	dobj	Polymerase,using
R2216	T3258	T3257	compound	Taq,Polymerase
R2217	T3259	T3260	punct	(,Invitrogen
R2218	T3260	T3257	parataxis	Invitrogen,Polymerase
R2219	T3261	T3260	punct	),Invitrogen
R222	T392	T393	preconj	either,reporters
R2220	T3262	T3231	punct	.,using
R2221	T3264	T3265	prep	For,were
R2222	T3265	T3271	ccomp	were,were
R2223	T3266	T3264	pobj	ECFP,For
R2224	T3267	T3266	cc	and,ECFP
R2225	T3268	T3266	conj	EYFP,ECFP
R2226	T3269	T3265	nsubj	primers,were
R2227	T3270	T3269	acl	used,primers
R2228	T3272	T3273	compound	GFP,L
R2229	T3273	T3265	attr	L,were
R223	T393	T391	pobj	reporters,of
R2230	T3274	T3273	punct	-,L
R2231	T3275	T3273	punct	(,L
R2232	T3276	T3277	nummod	5,GC
R2233	T3277	T3273	appos	GC,L
R2234	T3278	T3276	punct	',5
R2235	T3279	T3277	punct	-,GC
R2236	T3280	T3277	compound	TTG,GC
R2237	T3281	T3277	compound	AAT,GC
R2238	T3282	T3277	compound	TCG,GC
R2239	T3283	T3277	compound	CCA,GC
R224	T394	T393	det	the,reporters
R2240	T3284	T3277	compound	CCA,GC
R2241	T3285	T3277	compound	TGG,GC
R2242	T3286	T3277	compound	TGA,GC
R2243	T3287	T3273	punct	),L
R2244	T3288	T3273	cc	and,L
R2245	T3289	T3290	compound	GFP,R
R2246	T3290	T3273	conj	R,L
R2247	T3291	T3290	punct	-,R
R2248	T3292	T3293	punct	(,TCC
R2249	T3293	T3290	appos	TCC,R
R225	T395	T393	nmod	ECFP,reporters
R2250	T3294	T3293	nmod	5,TCC
R2251	T3295	T3294	punct	',5
R2252	T3296	T3293	punct	-,TCC
R2253	T3297	T3293	compound	TTG,TCC
R2254	T3298	T3293	compound	AAT,TCC
R2255	T3299	T3293	compound	TCT,TCC
R2256	T3300	T3293	compound	TAC,TCC
R2257	T3301	T3293	compound	TTG,TCC
R2258	T3302	T3293	compound	TAC,TCC
R2259	T3303	T3293	compound	AGC,TCC
R226	T396	T395	cc	or,ECFP
R2260	T3304	T3293	compound	TCG,TCC
R2261	T3305	T3271	punct	),were
R2262	T3306	T3271	punct	", ",were
R2263	T3307	T3271	prep	for,were
R2264	T3308	T3307	pobj	dsRed,for
R2265	T3309	T3271	nsubj	primers,were
R2266	T3310	T3311	compound	red,L
R2267	T3311	T3271	attr	L,were
R2268	T3312	T3311	punct	-,L
R2269	T3313	T3311	punct	(,L
R227	T397	T395	conj	EYFP,ECFP
R2270	T3314	T3315	nummod	5,C
R2271	T3315	T3311	appos	C,L
R2272	T3316	T3314	punct	',5
R2273	T3317	T3315	punct	-,C
R2274	T3318	T3315	compound	TTG,C
R2275	T3319	T3315	compound	AAT,C
R2276	T3320	T3315	compound	TCG,C
R2277	T3321	T3315	compound	CCA,C
R2278	T3322	T3315	compound	CCA,C
R2279	T3323	T3315	compound	TGG,C
R228	T398	T399	compound	GFP,variant
R2280	T3324	T3315	compound	TGC,C
R2281	T3325	T3315	compound	GCT,C
R2282	T3326	T3311	punct	),L
R2283	T3327	T3311	cc	and,L
R2284	T3328	T3329	compound	red,R
R2285	T3329	T3311	conj	R,L
R2286	T3330	T3329	punct	-,R
R2287	T3331	T3329	punct	(,R
R2288	T3332	T3333	nummod	5,G
R2289	T3333	T3329	appos	G,R
R229	T399	T393	compound	variant,reporters
R2290	T3334	T3332	punct	',5
R2291	T3335	T3333	punct	-,G
R2292	T3336	T3333	compound	TTG,G
R2293	T3337	T3333	compound	AAT,G
R2294	T3338	T3333	compound	TCT,G
R2295	T3339	T3333	compound	TAC,G
R2296	T3340	T3333	compound	GCT,G
R2297	T3341	T3333	compound	ACA,G
R2298	T3342	T3333	compound	GCT,G
R2299	T3343	T3333	compound	ACA,G
R23	T174	T172	pobj	animal,within
R230	T400	T399	punct	-,variant
R2300	T3344	T3333	compound	GGA,G
R2301	T3345	T3333	compound	ACA,G
R2302	T3346	T3333	compound	GGT,G
R2303	T3347	T3271	punct	),were
R2304	T3348	T3271	punct	.,were
R2305	T3350	T3351	det	All,primers
R2306	T3351	T3354	nsubj	primers,contain
R2307	T3352	T3351	nummod	5,primers
R2308	T3353	T3352	punct	',5
R2309	T3355	T3356	det	a,site
R231	T401	T372	punct	.,established
R2310	T3356	T3354	dobj	site,contain
R2311	T3357	T3358	compound	Kozak,consensus
R2312	T3358	T3356	compound	consensus,site
R2313	T3359	T3360	compound	translation,initiation
R2314	T3360	T3356	compound	initiation,site
R2315	T3361	T3356	amod	upstream,site
R2316	T3362	T3361	prep	of,upstream
R2317	T3363	T3364	det	the,ATG
R2318	T3364	T3362	pobj	ATG,of
R2319	T3365	T3354	prep	for,contain
R232	T403	T404	advmod	However,were
R2320	T3366	T3367	amod	increased,efficiency
R2321	T3367	T3365	pobj	efficiency,for
R2322	T3368	T3367	compound	translation,efficiency
R2323	T3369	T3367	prep	in,efficiency
R2324	T3370	T3371	amod	eukaryotic,cells
R2325	T3371	T3369	pobj	cells,in
R2326	T3372	T3373	punct	[,17
R2327	T3373	T3354	parataxis	17,contain
R2328	T3374	T3373	punct	],17
R2329	T3375	T3354	punct	.,contain
R233	T405	T404	punct	", ",were
R2330	T3377	T3378	aux	To,construct
R2331	T3378	T3379	advcl	construct,digested
R2332	T3380	T3378	dobj	vectors,construct
R2333	T3381	T3380	acl	driving,vectors
R2334	T3382	T3383	amod	ubiquitous,expression
R2335	T3383	T3381	dobj	expression,driving
R2336	T3384	T3383	compound	transgene,expression
R2337	T3385	T3381	prep	in,driving
R2338	T3386	T3387	compound	ES,cells
R2339	T3387	T3385	pobj	cells,in
R234	T406	T404	nsubj	we,were
R2340	T3388	T3387	cc	and,cells
R2341	T3389	T3387	conj	mice,cells
R2342	T3390	T3379	punct	", ",digested
R2343	T3391	T3392	det	the,cDNAs
R2344	T3392	T3379	nsubjpass	cDNAs,digested
R2345	T3393	T3394	npadvmod	PCR,amplified
R2346	T3394	T3392	amod	amplified,cDNAs
R2347	T3395	T3379	auxpass	were,digested
R2348	T3396	T3379	prep	with,digested
R2349	T3397	T3396	pobj	EcoRI,with
R235	T407	T404	acomp	unable,were
R2350	T3398	T3379	cc	and,digested
R2351	T3399	T3379	conj	inserted,digested
R2352	T3400	T3399	prep	into,inserted
R2353	T3401	T3402	det	the,sites
R2354	T3402	T3400	pobj	sites,into
R2355	T3403	T3402	compound	EcoRI,sites
R2356	T3404	T3402	prep	of,sites
R2357	T3405	T3404	pobj	pCAGGS,of
R2358	T3406	T3407	punct	[,18
R2359	T3407	T3399	parataxis	18,inserted
R236	T408	T409	aux	to,generate
R2360	T3408	T3407	punct	],18
R2361	T3409	T3410	aux	to,generate
R2362	T3410	T3399	advcl	generate,inserted
R2363	T3411	T3412	compound	pCX,ECFP
R2364	T3412	T3410	dobj	ECFP,generate
R2365	T3413	T3412	punct	-,ECFP
R2366	T3414	T3412	punct	", ",ECFP
R2367	T3415	T3416	compound	pCX,EYFP
R2368	T3416	T3412	conj	EYFP,ECFP
R2369	T3417	T3416	punct	-,EYFP
R237	T409	T407	xcomp	generate,unable
R2370	T3418	T3416	cc	and,EYFP
R2371	T3419	T3420	compound	pCX,DsRed
R2372	T3420	T3416	conj	DsRed,EYFP
R2373	T3421	T3420	punct	-,DsRed
R2374	T3422	T3379	punct	.,digested
R2375	T3424	T3425	nsubj	pCAGGS,contains
R2376	T3426	T3427	det	the,promoter
R2377	T3427	T3425	dobj	promoter,contains
R2378	T3428	T3427	compound	chicken,promoter
R2379	T3429	T3430	compound	beta,actin
R238	T410	T411	amod	equivalent,lines
R2380	T3430	T3427	compound	actin,promoter
R2381	T3431	T3430	punct	-,actin
R2382	T3432	T3427	cc	and,promoter
R2383	T3433	T3434	amod	first,intron
R2384	T3434	T3427	conj	intron,promoter
R2385	T3435	T3427	acl	coupled,promoter
R2386	T3436	T3435	prep	to,coupled
R2387	T3437	T3438	det	the,enhancer
R2388	T3438	T3436	pobj	enhancer,to
R2389	T3439	T3438	nmod	CMV,enhancer
R239	T411	T409	dobj	lines,generate
R2390	T3440	T3438	amod	immediate,enhancer
R2391	T3441	T3438	amod	early,enhancer
R2392	T3442	T3427	punct	", ",promoter
R2393	T3443	T3427	cc	and,promoter
R2394	T3444	T3445	compound	rabbit,signal
R2395	T3445	T3427	conj	signal,promoter
R2396	T3446	T3447	compound	beta,globin
R2397	T3447	T3445	compound	globin,signal
R2398	T3448	T3447	punct	-,globin
R2399	T3449	T3445	compound	polyadenylation,signal
R24	T175	T174	amod	single,animal
R240	T412	T411	compound	DsRed1,lines
R2400	T3450	T3425	punct	.,contains
R2401	T3452	T3453	det	This,combination
R2402	T3453	T3457	nsubjpass	combination,shown
R2403	T3454	T3455	compound	promoter,enhancer
R2404	T3455	T3453	compound	enhancer,combination
R2405	T3456	T3455	punct	/,enhancer
R2406	T3458	T3457	aux	has,shown
R2407	T3459	T3457	advmod	previously,shown
R2408	T3460	T3457	auxpass	been,shown
R2409	T3461	T3462	aux	to,drive
R241	T413	T404	punct	", ",were
R2410	T3462	T3457	xcomp	drive,shown
R2411	T3463	T3464	amod	strong,expression
R2412	T3464	T3462	dobj	expression,drive
R2413	T3465	T3463	cc	and,strong
R2414	T3466	T3463	conj	widespread,strong
R2415	T3467	T3464	compound	transgene,expression
R2416	T3468	T3462	prep	in,drive
R2417	T3469	T3470	compound	ES,cells
R2418	T3470	T3468	pobj	cells,in
R2419	T3471	T3470	punct	", ",cells
R242	T414	T404	advcl	suggesting,were
R2420	T3472	T3470	conj	embryos,cells
R2421	T3473	T3472	cc	and,embryos
R2422	T3474	T3475	amod	adult,mice
R2423	T3475	T3472	conj	mice,embryos
R2424	T3476	T3477	punct	[,19
R2425	T3477	T3457	parataxis	19,shown
R2426	T3478	T3477	nummod	18,19
R2427	T3479	T3477	punct	",",19
R2428	T3480	T3477	punct	],19
R2429	T3481	T3457	punct	.,shown
R243	T415	T416	mark	that,is
R2430	T3780	T3781	compound	Tissue,culture
R2431	T3783	T3784	compound	R1,cells
R2432	T3784	T3786	nsubjpass	cells,maintained
R2433	T3785	T3784	compound	ES,cells
R2434	T3787	T3788	punct	[,20
R2435	T3788	T3784	parataxis	20,cells
R2436	T3789	T3788	punct	],20
R2437	T3790	T3786	auxpass	were,maintained
R2438	T3791	T3786	prep	under,maintained
R2439	T3792	T3793	amod	standard,conditions
R244	T416	T414	advcl	is,suggesting
R2440	T3793	T3791	pobj	conditions,under
R2441	T3794	T3793	compound	culture,conditions
R2442	T3795	T3786	prep	in,maintained
R2443	T3796	T3797	det	the,presence
R2444	T3797	T3795	pobj	presence,in
R2445	T3798	T3797	prep	of,presence
R2446	T3799	T3798	pobj	LIF,of
R2447	T3800	T3786	punct	", ",maintained
R2448	T3801	T3802	mark	as,described
R2449	T3802	T3786	advcl	described,maintained
R245	T417	T416	nsubj	DsRed1,is
R2450	T3803	T3802	advmod	previously,described
R2451	T3804	T3805	punct	[,21
R2452	T3805	T3786	parataxis	21,maintained
R2453	T3806	T3805	punct	],21
R2454	T3807	T3786	punct	.,maintained
R2455	T3809	T3810	nummod	20,μg
R2456	T3810	T3811	nsubjpass	μg,co-electroporated
R2457	T3812	T3810	prep	of,μg
R2458	T3813	T3814	npadvmod	SalI,linearized
R2459	T3814	T3815	amod	linearized,ECFP
R246	T418	T416	neg	not,is
R2460	T3815	T3812	pobj	ECFP,of
R2461	T3816	T3815	compound	pCX,ECFP
R2462	T3817	T3815	punct	-,ECFP
R2463	T3818	T3815	punct	", ",ECFP
R2464	T3819	T3820	compound	pCX,EYFP
R2465	T3820	T3815	conj	EYFP,ECFP
R2466	T3821	T3820	punct	-,EYFP
R2467	T3822	T3820	cc	or,EYFP
R2468	T3823	T3824	compound	pCX,DsRed1
R2469	T3824	T3820	conj	DsRed1,EYFP
R247	T419	T420	advmod	developmentally,neutral
R2470	T3825	T3824	punct	-,DsRed1
R2471	T3826	T3811	auxpass	was,co-electroporated
R2472	T3827	T3811	prep	into,co-electroporated
R2473	T3828	T3829	quantmod	2,107
R2474	T3829	T3831	nummod	107,cells
R2475	T3830	T3829	punct	×,107
R2476	T3831	T3827	pobj	cells,into
R2477	T3832	T3811	prep	with,co-electroporated
R2478	T3833	T3834	quantmod	5,10
R2479	T3834	T3836	nummod	10,μg
R248	T420	T416	acomp	neutral,is
R2480	T3835	T3834	punct	–,10
R2481	T3836	T3832	pobj	μg,with
R2482	T3837	T3836	prep	of,μg
R2483	T3838	T3839	amod	circular,pPGKPuro
R2484	T3839	T3837	pobj	pPGKPuro,of
R2485	T3840	T3841	punct	[,22
R2486	T3841	T3811	parataxis	22,co-electroporated
R2487	T3842	T3841	punct	],22
R2488	T3843	T3811	punct	.,co-electroporated
R2489	T3845	T3846	nsubj	This,provided
R249	T421	T416	cc	or,is
R2490	T3847	T3848	amod	transient,resistance
R2491	T3848	T3846	dobj	resistance,provided
R2492	T3849	T3848	compound	drug,resistance
R2493	T3850	T3851	dep	which,used
R2494	T3851	T3848	relcl	used,resistance
R2495	T3852	T3851	aux	could,used
R2496	T3853	T3851	auxpass	be,used
R2497	T3854	T3851	prep	as,used
R2498	T3855	T3856	det	a,selection
R2499	T3856	T3854	pobj	selection,as
R25	T180	T181	amod	Non-invasive,reporters
R250	T422	T423	mark	that,sustained
R2500	T3857	T3856	prep	for,selection
R2501	T3858	T3857	pcomp	obtaining,for
R2502	T3859	T3860	amod	individual,colonies
R2503	T3860	T3858	dobj	colonies,obtaining
R2504	T3861	T3846	punct	.,provided
R2505	T3863	T3864	nsubjpass	Selection,applied
R2506	T3864	T3866	ccomp	applied,propagated
R2507	T3865	T3864	auxpass	was,applied
R2508	T3867	T3868	nummod	24,hours
R2509	T3868	T3869	npadvmod	hours,after
R251	T423	T416	conj	sustained,is
R2510	T3869	T3864	prep	after,applied
R2511	T3870	T3869	pobj	electroporation,after
R2512	T3871	T3864	cc	and,applied
R2513	T3872	T3864	conj	maintained,applied
R2514	T3873	T3872	prep	for,maintained
R2515	T3874	T3875	nummod	5,days
R2516	T3875	T3873	pobj	days,for
R2517	T3876	T3866	punct	", ",propagated
R2518	T3877	T3866	advmod	thereafter,propagated
R2519	T3878	T3866	nsubjpass	cells,propagated
R252	T424	T425	compound	transgene,expression
R2520	T3879	T3866	auxpass	were,propagated
R2521	T3880	T3866	prt	in,propagated
R2522	T3881	T3866	prep	in,propagated
R2523	T3882	T3883	det	the,absence
R2524	T3883	T3881	pobj	absence,in
R2525	T3884	T3883	prep	of,absence
R2526	T3885	T3884	pobj	selection,of
R2527	T3886	T3866	punct	.,propagated
R2528	T3888	T3889	amod	Fluorescent,colonies
R2529	T3889	T3890	nsubj	colonies,picked
R253	T425	T423	nsubjpass	expression,sustained
R2530	T3891	T3890	aux	were,picked
R2531	T3892	T3890	prep	into,picked
R2532	T3893	T3894	nummod	96,well
R2533	T3894	T3896	compound	well,plates
R2534	T3895	T3894	punct	-,well
R2535	T3896	T3892	pobj	plates,into
R2536	T3897	T3890	punct	", ",picked
R2537	T3898	T3890	conj	expanded,picked
R2538	T3899	T3898	cc	and,expanded
R2539	T3900	T3898	conj	archived,expanded
R254	T426	T423	aux	can,sustained
R2540	T3901	T3902	mark	as,described
R2541	T3902	T3890	advcl	described,picked
R2542	T3903	T3902	advmod	previously,described
R2543	T3904	T3902	punct	(,described
R2544	T3905	T3906	punct	[,2
R2545	T3906	T3902	parataxis	2,described
R2546	T3907	T3906	punct	],2
R2547	T3908	T3890	punct	.,picked
R2548	T3910	T3911	nsubjpass	Clones,screened
R2549	T3912	T3911	auxpass	were,screened
R255	T427	T423	neg	not,sustained
R2550	T3913	T3911	prep	for,screened
R2551	T3914	T3915	amod	strong,expression
R2552	T3915	T3913	pobj	expression,for
R2553	T3916	T3914	punct	", ",strong
R2554	T3917	T3914	conj	stable,strong
R2555	T3918	T3917	punct	", ",stable
R2556	T3919	T3917	cc	and,stable
R2557	T3920	T3917	conj	homogenous,stable
R2558	T3921	T3915	compound	transgene,expression
R2559	T3922	T3911	prep	during,screened
R256	T428	T423	auxpass	be,sustained
R2560	T3923	T3922	pobj	culture,during
R2561	T3924	T3911	prep	in,screened
R2562	T3925	T3926	det	the,absence
R2563	T3926	T3924	pobj	absence,in
R2564	T3927	T3926	prep	of,absence
R2565	T3928	T3929	compound	drug,selection
R2566	T3929	T3927	pobj	selection,of
R2567	T3930	T3911	punct	.,screened
R2568	T4217	T4218	compound	Chimera,production
R2569	T4219	T4218	cc	and,production
R257	T429	T423	advmod	constitutively,sustained
R2570	T4220	T4221	compound	germline,transmission
R2571	T4221	T4218	conj	transmission,production
R2572	T4223	T4224	compound	ES,cell
R2573	T4224	T4225	compound	cell,lines
R2574	T4225	T4226	nsubjpass	lines,assessed
R2575	T4227	T4225	acl	exhibiting,lines
R2576	T4228	T4229	amod	robust,expression
R2577	T4229	T4227	dobj	expression,exhibiting
R2578	T4230	T4229	compound	transgene,expression
R2579	T4231	T4232	advmod	in,vitro
R258	T430	T404	punct	.,were
R2580	T4232	T4227	advmod	vitro,exhibiting
R2581	T4233	T4226	auxpass	were,assessed
R2582	T4234	T4235	advmod	in,vivo
R2583	T4235	T4226	advmod	vivo,assessed
R2584	T4236	T4226	prep	for,assessed
R2585	T4237	T4238	det	the,extent
R2586	T4238	T4236	pobj	extent,for
R2587	T4239	T4238	amod	spatiotemporal,extent
R2588	T4240	T4238	prep	of,extent
R2589	T4241	T4242	compound	transgene,expression
R259	T432	T433	amod	Balanced,chimeras
R2590	T4242	T4240	pobj	expression,of
R2591	T4243	T4238	cc	and,extent
R2592	T4244	T4245	poss	their,potential
R2593	T4245	T4238	conj	potential,extent
R2594	T4246	T4245	amod	developmental,potential
R2595	T4247	T4245	prep	in,potential
R2596	T4248	T4247	pobj	chimeras,in
R2597	T4249	T4248	acl	produced,chimeras
R2598	T4250	T4249	prep	by,produced
R2599	T4251	T4250	pobj	aggregation,by
R26	T181	T183	nsubj	reporters,revolutionized
R260	T433	T450	nsubj	chimeras,demonstrate
R2600	T4252	T4251	prep	with,aggregation
R2601	T4253	T4254	amod	wild,type
R2602	T4254	T4255	nmod	type,embryos
R2603	T4255	T4252	pobj	embryos,with
R2604	T4256	T4255	amod	tetraploid,embryos
R2605	T4257	T4255	compound	ICR,embryos
R2606	T4258	T4259	advmod	so,produce
R2607	T4259	T4249	advcl	produce,produced
R2608	T4260	T4259	mark	as,produce
R2609	T4261	T4259	aux	to,produce
R261	T434	T435	punct	(,diploid
R2610	T4262	T4263	advmod	completely,derived
R2611	T4263	T4267	amod	derived,embryos
R2612	T4264	T4265	nmod	ES,cell
R2613	T4265	T4263	amod	cell,derived
R2614	T4266	T4263	punct	-,derived
R2615	T4267	T4259	dobj	embryos,produce
R2616	T4268	T4226	punct	", ",assessed
R2617	T4269	T4270	mark	as,described
R2618	T4270	T4226	advcl	described,assessed
R2619	T4271	T4270	advmod	previously,described
R262	T435	T432	parataxis	diploid,Balanced
R2620	T4272	T4273	punct	[,23
R2621	T4273	T4226	parataxis	23,assessed
R2622	T4274	T4273	nummod	20,23
R2623	T4275	T4273	punct	",",23
R2624	T4276	T4273	punct	],23
R2625	T4277	T4226	punct	.,assessed
R2626	T4279	T4280	det	A,protocol
R2627	T4280	T4282	nsubjpass	protocol,found
R2628	T4281	T4280	amod	detailed,protocol
R2629	T4283	T4282	aux	can,found
R263	T436	T435	nsubj	diploid,diploid
R2630	T4284	T4282	auxpass	be,found
R2631	T4285	T4282	prep	on,found
R2632	T4286	T4287	det	the,web
R2633	T4287	T4285	pobj	web,on
R2634	T4288	T4289	npadvmod	world,wide
R2635	T4289	T4287	amod	wide,web
R2636	T4290	T4282	prep	at,found
R2637	T4291	T4282	punct	.,found
R2638	T4293	T4294	nsubjpass	Clones,used
R2639	T4295	T4293	acl	giving,Clones
R264	T437	T435	punct	<,diploid
R2640	T4296	T4297	amod	fluorescent,embryos
R2641	T4297	T4295	dobj	embryos,giving
R2642	T4298	T4297	compound	E12.5,embryos
R2643	T4299	T4297	acl	having,embryos
R2644	T4300	T4301	neg	no,malformations
R2645	T4301	T4299	dobj	malformations,having
R2646	T4302	T4303	advmod	readily,identifiable
R2647	T4303	T4301	amod	identifiable,malformations
R2648	T4304	T4294	auxpass	were,used
R2649	T4305	T4306	aux	to,generate
R265	T438	T435	punct	-,diploid
R2650	T4306	T4294	advcl	generate,used
R2651	T4307	T4308	npadvmod	germline,transmitting
R2652	T4308	T4309	amod	transmitting,chimeras
R2653	T4309	T4306	dobj	chimeras,generate
R2654	T4310	T4294	prep	by,used
R2655	T4311	T4310	pobj	aggregation,by
R2656	T4312	T4311	prep	with,aggregation
R2657	T4313	T4314	amod	diploid,embryos
R2658	T4314	T4312	pobj	embryos,with
R2659	T4315	T4316	amod	wild,type
R266	T439	T435	punct	>,diploid
R2660	T4316	T4314	compound	type,embryos
R2661	T4317	T4314	compound	ICR,embryos
R2662	T4318	T4319	mark	as,described
R2663	T4319	T4294	advcl	described,used
R2664	T4320	T4319	advmod	previously,described
R2665	T4321	T4322	punct	[,23
R2666	T4322	T4294	parataxis	23,used
R2667	T4323	T4322	nummod	21,23
R2668	T4324	T4322	punct	",",23
R2669	T4325	T4322	punct	],23
R267	T440	T435	punct	),diploid
R2670	T4326	T4294	punct	.,used
R2671	T4328	T4329	prep	After,bred
R2672	T4330	T4331	compound	germline,transmission
R2673	T4331	T4328	pobj	transmission,After
R2674	T4332	T4329	nsubjpass	transgenes,bred
R2675	T4333	T4329	auxpass	were,bred
R2676	T4334	T4329	prep	to,bred
R2677	T4335	T4334	pobj	homozygosity,to
R2678	T4336	T4329	punct	.,bred
R2679	T4338	T4339	nsubjpass	Transgenes,maintained
R268	T441	T432	cc	and,Balanced
R2680	T4340	T4339	auxpass	were,maintained
R2681	T4341	T4339	prep	on,maintained
R2682	T4342	T4343	preconj	both,ICR
R2683	T4343	T4345	nmod	ICR,backgrounds
R2684	T4344	T4343	amod	mixedbred,ICR
R2685	T4345	T4341	pobj	backgrounds,on
R2686	T4346	T4343	cc	and,ICR
R2687	T4347	T4348	nmod	inbred,SvJ
R2688	T4348	T4343	conj	SvJ,ICR
R2689	T4349	T4348	compound	129,SvJ
R269	T442	T432	conj	polarized,Balanced
R2690	T4350	T4348	punct	/,SvJ
R2691	T4351	T4345	amod	genetic,backgrounds
R2692	T4352	T4353	punct	[,24
R2693	T4353	T4339	parataxis	24,maintained
R2694	T4354	T4353	punct	],24
R2695	T4355	T4339	punct	.,maintained
R2696	T4641	T4642	nsubjpass	Photographs,taken
R2697	T4643	T4641	prep	of,Photographs
R2698	T4644	T4645	amod	live,samples
R2699	T4645	T4643	pobj	samples,of
R27	T182	T181	amod	autofluorescent,reporters
R270	T443	T444	punct	(,diploid
R2700	T4646	T4645	punct	", ",samples
R2701	T4647	T4648	advmod	freshly,dissected
R2702	T4648	T4645	acl	dissected,samples
R2703	T4649	T4648	prep	in,dissected
R2704	T4650	T4649	pobj	PBS,in
R2705	T4651	T4652	punct	(,24
R2706	T4652	T4641	parataxis	24,Photographs
R2707	T4653	T4652	punct	[,24
R2708	T4654	T4652	punct	],24
R2709	T4655	T4642	auxpass	were,taken
R271	T444	T442	parataxis	diploid,polarized
R2710	T4656	T4642	prep	on,taken
R2711	T4657	T4658	det	a,microscope
R2712	T4658	T4656	pobj	microscope,on
R2713	T4659	T4658	compound	Leica,microscope
R2714	T4660	T4658	compound	MZFLIII,microscope
R2715	T4661	T4658	compound	stereo,microscope
R2716	T4662	T4658	compound	dissecting,microscope
R2717	T4663	T4658	acl	equipped,microscope
R2718	T4664	T4663	prep	with,equipped
R2719	T4665	T4666	compound	epifluorescence,optics
R272	T445	T444	nsubj	tetraploid,diploid
R2720	T4666	T4664	pobj	optics,with
R2721	T4667	T4642	punct	.,taken
R2722	T4669	T4670	compound	Filter,sets
R2723	T4670	T4671	nsubj	sets,were
R2724	T4672	T4670	acl	used,sets
R2725	T4673	T4671	punct	: ,were
R2726	T4674	T4675	nmod	excitation,nm
R2727	T4675	T4671	attr	nm,were
R2728	T4676	T4677	nummod	436,20
R2729	T4677	T4675	nummod	20,nm
R273	T446	T444	punct	<,diploid
R2730	T4678	T4677	punct	/,20
R2731	T4679	T4675	punct	", ",nm
R2732	T4680	T4681	amod	dichroic,nm
R2733	T4681	T4675	conj	nm,nm
R2734	T4682	T4681	nmod	mirror,nm
R2735	T4683	T4681	nummod	455,nm
R2736	T4684	T4681	punct	", ",nm
R2737	T4685	T4686	nmod	barrier,nm
R2738	T4686	T4681	conj	nm,nm
R2739	T4687	T4688	nummod	480,40
R274	T447	T444	punct	-,diploid
R2740	T4688	T4686	nummod	40,nm
R2741	T4689	T4688	punct	/,40
R2742	T4690	T4686	prep	for,nm
R2743	T4691	T4690	pobj	ECFP,for
R2744	T4692	T4686	punct	", ",nm
R2745	T4693	T4694	nmod	excitation,nm
R2746	T4694	T4686	conj	nm,nm
R2747	T4695	T4696	nummod	500,20
R2748	T4696	T4694	nummod	20,nm
R2749	T4697	T4696	punct	/,20
R275	T448	T444	punct	>,diploid
R2750	T4698	T4694	punct	", ",nm
R2751	T4699	T4700	amod	dichroic,nm
R2752	T4700	T4694	conj	nm,nm
R2753	T4701	T4700	nmod	mirror,nm
R2754	T4702	T4700	nummod	515,nm
R2755	T4703	T4700	punct	", ",nm
R2756	T4704	T4705	nmod	barrier,nm
R2757	T4705	T4700	conj	nm,nm
R2758	T4706	T4707	nummod	535,30
R2759	T4707	T4705	nummod	30,nm
R276	T449	T444	punct	),diploid
R2760	T4708	T4707	punct	/,30
R2761	T4709	T4705	prep	for,nm
R2762	T4710	T4709	pobj	EYFP,for
R2763	T4711	T4705	cc	and,nm
R2764	T4712	T4713	nmod	excitation,nm
R2765	T4713	T4705	conj	nm,nm
R2766	T4714	T4715	nummod	540,25
R2767	T4715	T4713	nummod	25,nm
R2768	T4716	T4715	punct	/,25
R2769	T4717	T4713	punct	", ",nm
R277	T451	T433	acl	comprising,chimeras
R2770	T4718	T4719	amod	dichroic,nm
R2771	T4719	T4713	conj	nm,nm
R2772	T4720	T4719	nmod	mirror,nm
R2773	T4721	T4719	nummod	565,nm
R2774	T4722	T4719	punct	", ",nm
R2775	T4723	T4719	conj	barrier,nm
R2776	T4724	T4723	nummod	605,barrier
R2777	T4725	T4723	punct	/,barrier
R2778	T4726	T4723	nummod	55,barrier
R2779	T4727	T4723	prep	for,barrier
R278	T452	T451	dobj	combinations,comprising
R2780	T4728	T4727	pobj	DsRed,for
R2781	T4729	T4671	punct	.,were
R2782	T4731	T4732	compound	Adult,mice
R2783	T4732	T4733	nsubjpass	mice,anaesthetized
R2784	T4734	T4733	auxpass	were,anaesthetized
R2785	T4735	T4733	prep	with,anaesthetized
R2786	T4736	T4735	pobj	avertin,with
R2787	T4737	T4733	punct	.,anaesthetized
R2788	T4739	T4740	nsubjpass	They,placed
R2789	T4741	T4740	auxpass	were,placed
R279	T453	T452	prep	of,combinations
R2790	T4742	T4740	advmod	then,placed
R2791	T4743	T4740	prep	on,placed
R2792	T4744	T4745	det	the,stage
R2793	T4745	T4743	pobj	stage,on
R2794	T4746	T4745	prep	of,stage
R2795	T4747	T4748	det	a,microscope
R2796	T4748	T4746	pobj	microscope,of
R2797	T4749	T4748	nmod	stereo,microscope
R2798	T4750	T4748	amod	dissecting,microscope
R2799	T4751	T4748	punct	(,microscope
R28	T184	T183	aux	have,revolutionized
R280	T454	T455	det	the,ECFP
R2800	T4752	T4753	compound	Leica,MZFLIII
R2801	T4753	T4748	appos	MZFLIII,microscope
R2802	T4754	T4748	punct	),microscope
R2803	T4755	T4756	prep	from,removed
R2804	T4756	T4748	relcl	removed,microscope
R2805	T4757	T4755	pobj	which,from
R2806	T4758	T4759	det	the,objective
R2807	T4759	T4756	nsubjpass	objective,removed
R2808	T4760	T4756	aux	had,removed
R2809	T4761	T4756	auxpass	been,removed
R281	T455	T453	pobj	ECFP,of
R2810	T4762	T4740	punct	", ",placed
R2811	T4763	T4764	amod	such,illuminated
R2812	T4764	T4740	advcl	illuminated,placed
R2813	T4765	T4764	mark	that,illuminated
R2814	T4766	T4764	nsubjpass	they,illuminated
R2815	T4767	T4764	auxpass	were,illuminated
R2816	T4768	T4764	advcl	using,illuminated
R2817	T4769	T4770	det	the,optics
R2818	T4770	T4768	dobj	optics,using
R2819	T4771	T4770	compound	epifluorescence,optics
R282	T456	T455	cc	and,ECFP
R2820	T4772	T4770	punct	(,optics
R2821	T4773	T4774	nmod	lamp,filters
R2822	T4774	T4770	appos	filters,optics
R2823	T4775	T4773	cc	and,lamp
R2824	T4776	T4773	conj	excitation,lamp
R2825	T4777	T4740	punct	),placed
R2826	T4778	T4740	punct	.,placed
R2827	T4780	T4781	nsubjpass	Photographs,taken
R2828	T4782	T4781	auxpass	were,taken
R2829	T4783	T4781	advcl	using,taken
R283	T457	T455	conj	EYFP,ECFP
R2830	T4784	T4785	det	a,camera
R2831	T4785	T4783	dobj	camera,using
R2832	T4786	T4785	nmod	Nikon,camera
R2833	T4787	T4785	nmod	FE10,camera
R2834	T4788	T4789	nummod	35,mm
R2835	T4789	T4785	compound	mm,camera
R2836	T4790	T4785	compound	SLR,camera
R2837	T4791	T4785	acl	fitted,camera
R2838	T4792	T4791	prep	with,fitted
R2839	T4793	T4794	amod	individual,filters
R284	T458	T459	compound	ES,cells
R2840	T4794	T4792	pobj	filters,with
R2841	T4795	T4794	compound	emission,filters
R2842	T4796	T4781	punct	.,taken
R2843	T4798	T4799	nsubjpass	Images,using
R2844	T4800	T4799	auxpass	were,using
R2845	T4801	T4799	aux	processed,using
R2846	T4802	T4803	compound	Photoshop,software
R2847	T4803	T4799	dobj	software,using
R2848	T4804	T4805	punct	(,Systems
R2849	T4805	T4803	parataxis	Systems,software
R285	T459	T455	appos	cells,ECFP
R2850	T4806	T4805	compound	Adobe,Systems
R2851	T4807	T4805	punct	),Systems
R2852	T4808	T4799	punct	.,using
R2853	T4929	T4930	det	The,mice
R2854	T4930	T4938	nsubjpass	mice,acquired
R2855	T4931	T4932	amod	cyan,protein
R2856	T4932	T4936	npadvmod	protein,expressing
R2857	T4933	T4931	cc	and,cyan
R2858	T4934	T4931	conj	yellow,cyan
R2859	T4935	T4932	amod	fluorescent,protein
R286	T460	T459	cc	and,cells
R2860	T4936	T4930	amod	expressing,mice
R2861	T4937	T4936	punct	-,expressing
R2862	T4939	T4930	acl	described,mice
R2863	T4940	T4939	advmod	here,described
R2864	T4941	T4938	aux	have,acquired
R2865	T4942	T4938	auxpass	been,acquired
R2866	T4943	T4938	prep	by,acquired
R2867	T4944	T4938	punct	", ",acquired
R2868	T4945	T4938	cc	and,acquired
R2869	T4946	T4938	conj	are,acquired
R287	T461	T460	punct	/,and
R2870	T4947	T4946	acomp	available,are
R2871	T4948	T4947	prep	from,available
R2872	T4949	T4946	punct	", ",are
R2873	T4950	T4951	det	the,Resource
R2874	T4951	T4946	conj	Resource,are
R2875	T4952	T4953	compound	Jackson,Laboratory
R2876	T4953	T4951	compound	Laboratory,Resource
R2877	T4954	T4955	compound	Induced,Mutant
R2878	T4955	T4951	compound	Mutant,Resource
R2879	T4956	T4938	punct	.,acquired
R288	T462	T460	cc	or,and
R2880	T4958	T4959	nsubjpass	They,referred
R2881	T4960	T4959	auxpass	are,referred
R2882	T4961	T4959	prep	to,referred
R2883	T4962	T4959	advmod	therein,referred
R2884	T4963	T4959	prep	as,referred
R2885	T4964	T4965	nummod	003373,Stock
R2886	T4965	T4963	pobj	Stock,as
R2887	T4966	T4965	appos	TgN,Stock
R2888	T4967	T4966	punct	(,TgN
R2889	T4968	T4966	appos	ActbECFP,TgN
R289	T463	T459	conj	embryos,cells
R2890	T4969	T4965	punct	),Stock
R2891	T4970	T4965	appos	1Nagy,Stock
R2892	T4971	T4970	punct	(,1Nagy
R2893	T4972	T4973	compound	CK6,ECFP
R2894	T4973	T4970	appos	ECFP,1Nagy
R2895	T4974	T4973	punct	/,ECFP
R2896	T4975	T4965	punct	),Stock
R2897	T4976	T4965	cc	and,Stock
R2898	T4977	T4978	nummod	003772,Stock
R2899	T4978	T4965	conj	Stock,Stock
R29	T185	T186	compound	lineage,labeling
R290	T464	T450	punct	", ",demonstrate
R2900	T4979	T4978	appos	TgN,Stock
R2901	T4980	T4979	punct	(,TgN
R2902	T4981	T4979	appos	ActbEYFP,TgN
R2903	T4982	T4978	punct	),Stock
R2904	T4983	T4978	appos	1Nagy,Stock
R2905	T4984	T4983	punct	(,1Nagy
R2906	T4985	T4986	compound	YC5,EYFP
R2907	T4986	T4983	appos	EYFP,1Nagy
R2908	T4987	T4986	punct	/,EYFP
R2909	T4988	T4959	punct	),referred
R291	T465	T466	mark	that,distinguished
R2910	T4989	T4959	punct	.,referred
R2912	T5231	T5232	amod	Non-invasive,visualization
R2913	T5233	T5234	amod	multiple,reporter
R2914	T5234	T5232	compound	reporter,visualization
R2915	T5235	T5232	prep	in,visualization
R2916	T5236	T5237	compound	ES,cells
R2917	T5237	T5235	pobj	cells,in
R2918	T5238	T5232	punct	.,visualization
R2919	T5240	T5241	punct	(,a
R292	T466	T450	ccomp	distinguished,demonstrate
R2920	T5241	T5242	meta	a,mixture
R2921	T5243	T5241	punct	-,a
R2922	T5244	T5241	dep	d,a
R2923	T5245	T5241	punct	),a
R2924	T5246	T5242	det	a,mixture
R2925	T5247	T5242	prep	of,mixture
R2926	T5248	T5249	nmod	CK6,ECFP
R2927	T5249	T5251	nmod	ECFP,cells
R2928	T5250	T5249	punct	/,ECFP
R2929	T5251	T5247	pobj	cells,of
R293	T467	T466	nsubjpass	populations,distinguished
R2930	T5252	T5253	punct	(,ECFP+
R2931	T5253	T5249	parataxis	ECFP+,ECFP
R2932	T5254	T5253	punct	),ECFP+
R2933	T5255	T5249	cc	and,ECFP
R2934	T5256	T5257	compound	YC5,EYFP
R2935	T5257	T5249	conj	EYFP,ECFP
R2936	T5258	T5257	punct	/,EYFP
R2937	T5259	T5260	punct	(,EYFP+
R2938	T5260	T5257	parataxis	EYFP+,EYFP
R2939	T5261	T5260	punct	),EYFP+
R294	T468	T467	prep	of,populations
R2940	T5262	T5251	compound	ES,cells
R2941	T5263	T5242	prep	at,mixture
R2942	T5264	T5265	amod	low,density
R2943	T5265	T5263	pobj	density,at
R2944	T5266	T5242	punct	.,mixture
R2945	T5268	T5269	punct	(,a
R2946	T5269	T5270	meta	a,image
R2947	T5271	T5269	punct	),a
R2948	T5272	T5270	acl	taken,image
R2949	T5273	T5272	prep	under,taken
R295	T469	T468	pobj	cells,of
R2950	T5274	T5275	amod	bright,field
R2951	T5275	T5273	pobj	field,under
R2952	T5276	T5272	prep	with,taken
R2953	T5277	T5278	det	no,epifluorescence
R2954	T5278	T5276	pobj	epifluorescence,with
R2955	T5279	T5270	punct	.,image
R2956	T5281	T5282	punct	(,b
R2957	T5282	T5283	meta	b,image
R2958	T5284	T5282	punct	),b
R2959	T5285	T5286	amod	dark,field
R296	T470	T467	acl	expressing,populations
R2960	T5286	T5283	compound	field,image
R2961	T5287	T5283	acl	taken,image
R2962	T5288	T5287	prep	through,taken
R2963	T5289	T5290	det	an,filter
R2964	T5290	T5288	pobj	filter,through
R2965	T5291	T5290	compound	EYFP,filter
R2966	T5292	T5283	punct	.,image
R2967	T5294	T5295	punct	(,c
R2968	T5295	T5296	meta	c,image
R2969	T5297	T5295	punct	),c
R297	T471	T472	det	each,reporter
R2970	T5298	T5299	nmod	dark,field
R2971	T5299	T5296	compound	field,image
R2972	T5300	T5296	acl	taken,image
R2973	T5301	T5300	prep	through,taken
R2974	T5302	T5303	det	an,filter
R2975	T5303	T5301	pobj	filter,through
R2976	T5304	T5303	compound	ECFP,filter
R2977	T5305	T5296	punct	.,image
R2978	T5307	T5308	punct	(,d
R2979	T5308	T5309	meta	d,image
R298	T472	T470	dobj	reporter,expressing
R2980	T5310	T5308	punct	),d
R2981	T5311	T5312	amod	dark,field
R2982	T5312	T5309	nmod	field,image
R2983	T5313	T5314	amod	double,exposure
R2984	T5314	T5309	compound	exposure,image
R2985	T5315	T5309	acl	acquired,image
R2986	T5316	T5315	prep	by,acquired
R2987	T5317	T5318	advmod	consecutively,using
R2988	T5318	T5316	pcomp	using,by
R2989	T5319	T5320	nmod	ECFP,filters
R299	T473	T472	amod	individual,reporter
R2990	T5320	T5318	dobj	filters,using
R2991	T5321	T5319	cc	and,ECFP
R2992	T5322	T5319	conj	EYFP,ECFP
R2993	T5323	T5309	punct	.,image
R2994	T5325	T5326	amod	ECFP+,cells
R2995	T5326	T5327	nsubjpass	cells,discerned
R2996	T5328	T5327	aux	can,discerned
R2997	T5329	T5327	advmod	clearly,discerned
R2998	T5330	T5327	auxpass	be,discerned
R2999	T5331	T5327	prep	from,discerned
R30	T186	T183	dobj	labeling,revolutionized
R300	T474	T466	aux	can,distinguished
R3000	T5332	T5333	amod	EYFP+,cells
R3001	T5333	T5331	pobj	cells,from
R3002	T5334	T5327	punct	.,discerned
R3003	T5336	T5337	punct	(,e
R3004	T5337	T5338	meta	e,culture
R3005	T5339	T5337	punct	-,e
R3006	T5340	T5337	dep	f,e
R3007	T5341	T5337	punct	),e
R3008	T5342	T5338	prep	of,culture
R3009	T5343	T5344	amod	mixed,populations
R301	T475	T466	auxpass	be,distinguished
R3010	T5344	T5342	pobj	populations,of
R3011	T5345	T5344	prep	of,populations
R3012	T5346	T5347	compound	ES,cells
R3013	T5347	T5345	pobj	cells,of
R3014	T5348	T5338	acl	comprising,culture
R3015	T5349	T5350	amod	ECFP+,cells
R3016	T5350	T5348	dobj	cells,comprising
R3017	T5351	T5349	punct	", ",ECFP+
R3018	T5352	T5349	conj	EYFP+,ECFP+
R3019	T5353	T5352	cc	and,EYFP+
R302	T476	T466	prep	within,distinguished
R3020	T5354	T5352	conj	RFP+,EYFP+
R3021	T5355	T5338	punct	.,culture
R3022	T5357	T5358	compound	Scale,bars
R3023	T5358	T5359	nsubj	bars,represent
R3024	T5360	T5361	nummod	100,μm
R3025	T5361	T5359	dobj	μm,represent
R3026	T5362	T5359	punct	.,represent
R3027	T5432	T5433	amod	Dual,visualization
R3028	T5434	T5433	amod	non-invasive,visualization
R3029	T5435	T5433	compound	reporter,visualization
R303	T477	T478	det	a,animal
R3030	T5436	T5433	prep	in,visualization
R3031	T5437	T5436	pobj	embryos,in
R3032	T5438	T5437	cc	and,embryos
R3033	T5439	T5440	amod	adult,mice
R3034	T5440	T5437	conj	mice,embryos
R3035	T5441	T5433	punct	.,visualization
R3036	T5443	T5444	amod	Transgenic,animals
R3037	T5444	T5445	nsubj	animals,are
R3038	T5446	T5445	acomp	hemizygous,are
R3039	T5447	T5445	prep	for,are
R304	T478	T476	pobj	animal,within
R3040	T5448	T5449	preconj	either,transgenes
R3041	T5449	T5447	pobj	transgenes,for
R3042	T5450	T5449	det	the,transgenes
R3043	T5451	T5452	nmod	CK6,ECFP
R3044	T5452	T5449	nmod	ECFP,transgenes
R3045	T5453	T5452	punct	/,ECFP
R3046	T5454	T5452	cc	or,ECFP
R3047	T5455	T5456	compound	YC5,EYFP
R3048	T5456	T5452	conj	EYFP,ECFP
R3049	T5457	T5456	punct	/,EYFP
R305	T479	T478	amod	single,animal
R3050	T5458	T5445	punct	.,are
R3051	T5460	T5461	punct	(,a
R3052	T5461	T5462	meta	a,embryos
R3053	T5463	T5461	punct	-,a
R3054	T5464	T5461	dep	d,a
R3055	T5465	T5461	punct	),a
R3056	T5466	T5462	nmod	blastocyst,embryos
R3057	T5467	T5462	nmod	stage,embryos
R3058	T5468	T5469	punct	(,E3.5
R3059	T5469	T5462	parataxis	E3.5,embryos
R306	T480	T450	punct	.,demonstrate
R3060	T5470	T5469	punct	),E3.5
R3061	T5471	T5462	punct	", ",embryos
R3062	T5472	T5473	dep	that,are
R3063	T5473	T5462	relcl	are,embryos
R3064	T5474	T5475	advmod	either,fluorescent
R3065	T5475	T5473	acomp	fluorescent,are
R3066	T5476	T5475	cc	or,fluorescent
R3067	T5477	T5475	conj	non-fluorescent,fluorescent
R3068	T5478	T5462	punct	.,embryos
R3069	T5480	T5481	amod	Fluorescent,embryos
R307	T484	T485	nmod	GFP,reporters
R3070	T5481	T5482	nsubj	embryos,are
R3071	T5483	T5484	preconj	either,cyan
R3072	T5484	T5482	acomp	cyan,are
R3073	T5485	T5484	amod	blue,cyan
R3074	T5486	T5484	punct	/,cyan
R3075	T5487	T5484	cc	or,cyan
R3076	T5488	T5489	amod	yellow,green
R3077	T5489	T5484	conj	green,cyan
R3078	T5490	T5489	punct	/,green
R3079	T5491	T5482	punct	", ",are
R308	T485	T487	nsubj	reporters,are
R3080	T5492	T5493	advmod	thereby,being
R3081	T5493	T5482	advcl	being,are
R3082	T5494	T5493	acomp	hemizygous,being
R3083	T5495	T5493	prep	for,being
R3084	T5496	T5497	preconj	either,ECFP
R3085	T5497	T5501	nmod	ECFP,transgenes
R3086	T5498	T5497	det	the,ECFP
R3087	T5499	T5497	nmod	CK6,ECFP
R3088	T5500	T5497	punct	/,ECFP
R3089	T5501	T5495	pobj	transgenes,for
R309	T486	T484	amod	variant,GFP
R3090	T5502	T5497	cc	or,ECFP
R3091	T5503	T5504	compound	YC5,EYFP
R3092	T5504	T5497	conj	EYFP,ECFP
R3093	T5505	T5504	punct	/,EYFP
R3094	T5506	T5493	advmod	respectively,being
R3095	T5507	T5482	punct	.,are
R3096	T5509	T5510	amod	Non-fluorescent,embryos
R3097	T5510	T5511	nsubj	embryos,are
R3098	T5512	T5510	punct	(,embryos
R3099	T5513	T5510	acl	marked,embryos
R31	T187	T183	prep	in,revolutionized
R310	T488	T487	acomp	unique,are
R3100	T5514	T5513	prep	with,marked
R3101	T5515	T5516	det	an,asterix
R3102	T5516	T5514	pobj	asterix,with
R3103	T5517	T5513	prep	on,marked
R3104	T5518	T5519	compound	panel,a
R3105	T5519	T5517	pobj	a,on
R3106	T5520	T5511	punct	),are
R3107	T5521	T5511	prep	of,are
R3108	T5522	T5523	det	the,strain
R3109	T5523	T5521	pobj	strain,of
R311	T489	T488	prep	in,unique
R3110	T5524	T5525	amod	wild,type
R3111	T5525	T5523	nmod	type,strain
R3112	T5526	T5523	amod	outbred,strain
R3113	T5527	T5523	compound	ICR,strain
R3114	T5528	T5511	punct	.,are
R3115	T5530	T5531	punct	(,a
R3116	T5531	T5532	meta	a,image
R3117	T5533	T5531	punct	),a
R3118	T5534	T5532	acl	taken,image
R3119	T5535	T5534	prep	under,taken
R312	T490	T489	pcomp	allowing,in
R3120	T5536	T5537	amod	bright,field
R3121	T5537	T5535	pobj	field,under
R3122	T5538	T5534	prep	with,taken
R3123	T5539	T5540	det	no,epifluorescence
R3124	T5540	T5538	pobj	epifluorescence,with
R3125	T5541	T5532	punct	.,image
R3126	T5543	T5544	punct	(,b
R3127	T5544	T5545	meta	b,image
R3128	T5546	T5544	punct	),b
R3129	T5547	T5548	amod	dark,field
R313	T491	T492	amod	non-invasive,visualization
R3130	T5548	T5545	compound	field,image
R3131	T5549	T5545	acl	taken,image
R3132	T5550	T5549	prep	through,taken
R3133	T5551	T5552	det	an,filter
R3134	T5552	T5550	pobj	filter,through
R3135	T5553	T5552	compound	EYFP,filter
R3136	T5554	T5545	punct	.,image
R3137	T5556	T5557	punct	(,c
R3138	T5557	T5558	meta	c,image
R3139	T5559	T5557	punct	),c
R314	T492	T490	dobj	visualization,allowing
R3140	T5560	T5561	nmod	dark,field
R3141	T5561	T5558	compound	field,image
R3142	T5562	T5558	acl	taken,image
R3143	T5563	T5562	prep	through,taken
R3144	T5564	T5565	det	an,filter
R3145	T5565	T5563	pobj	filter,through
R3146	T5566	T5565	compound	ECFP,filter
R3147	T5567	T5558	punct	.,image
R3148	T5569	T5570	punct	(,d
R3149	T5570	T5571	meta	d,image
R315	T493	T492	amod	multi-spectral,visualization
R3150	T5572	T5570	punct	),d
R3151	T5573	T5574	amod	dark,field
R3152	T5574	T5571	nmod	field,image
R3153	T5575	T5576	amod	double,exposure
R3154	T5576	T5571	compound	exposure,image
R3155	T5577	T5571	acl	acquired,image
R3156	T5578	T5577	prep	by,acquired
R3157	T5579	T5580	advmod	consecutively,using
R3158	T5580	T5578	pcomp	using,by
R3159	T5581	T5582	nmod	ECFP,filters
R316	T494	T490	prep	in,allowing
R3160	T5582	T5580	dobj	filters,using
R3161	T5583	T5581	cc	and,ECFP
R3162	T5584	T5581	conj	EYFP,ECFP
R3163	T5585	T5571	punct	.,image
R3164	T5587	T5588	punct	(,e
R3165	T5588	T5589	meta	e,is
R3166	T5590	T5588	punct	-,e
R3167	T5591	T5588	dep	h,e
R3168	T5592	T5588	punct	),e
R3169	T5593	T5594	nummod	two,embryos
R317	T495	T496	amod	live,samples
R3170	T5594	T5589	dep	embryos,is
R3171	T5595	T5594	amod	transgenic,embryos
R3172	T5596	T5597	punct	(,TG
R3173	T5597	T5595	parataxis	TG,transgenic
R3174	T5598	T5597	punct	/,TG
R3175	T5599	T5597	punct	+,TG
R3176	T5600	T5597	punct	),TG
R3177	T5601	T5594	compound	E13.5,embryos
R3178	T5602	T5594	acl	dissected,embryos
R3179	T5603	T5602	advcl	free,dissected
R318	T496	T494	pobj	samples,in
R3180	T5604	T5603	prep	of,free
R3181	T5605	T5606	poss	their,membranes
R3182	T5606	T5604	pobj	membranes,of
R3183	T5607	T5606	amod	extraembryonic,membranes
R3184	T5608	T5589	punct	", ",is
R3185	T5609	T5589	nsubj	one,is
R3186	T5610	T5611	amod	blue,cyan
R3187	T5611	T5613	amod	cyan,fluorescent
R3188	T5612	T5611	punct	/,cyan
R3189	T5613	T5589	acomp	fluorescent,is
R319	T497	T487	punct	.,are
R3190	T5614	T5615	punct	(,left
R3191	T5615	T5589	parataxis	left,is
R3192	T5616	T5617	compound	CK6,ECFP
R3193	T5617	T5619	npadvmod	ECFP,transgenic
R3194	T5618	T5617	punct	/,ECFP
R3195	T5619	T5615	amod	transgenic,left
R3196	T5620	T5615	punct	", ",left
R3197	T5621	T5615	punct	),left
R3198	T5622	T5589	cc	and,is
R3199	T5623	T5624	det	the,other
R32	T188	T189	det	an,array
R320	T499	T500	det	The,cells
R3200	T5624	T5625	nsubj	other,is
R3201	T5625	T5589	conj	is,is
R3202	T5626	T5627	amod	yellow,green
R3203	T5627	T5629	amod	green,fluorescent
R3204	T5628	T5627	punct	/,green
R3205	T5629	T5625	acomp	fluorescent,is
R3206	T5630	T5631	punct	(,right
R3207	T5631	T5625	parataxis	right,is
R3208	T5632	T5633	compound	7YC5,EYFP
R3209	T5633	T5635	npadvmod	EYFP,transgenic
R321	T500	T508	nsubj	cells,provide
R3210	T5634	T5633	punct	/,EYFP
R3211	T5635	T5631	amod	transgenic,right
R3212	T5636	T5631	punct	", ",right
R3213	T5637	T5631	punct	),right
R3214	T5638	T5589	punct	.,is
R3215	T5640	T5641	punct	(,e
R3216	T5641	T5642	meta	e,image
R3217	T5643	T5641	punct	),e
R3218	T5644	T5642	acl	taken,image
R3219	T5645	T5644	prep	under,taken
R322	T501	T502	npadvmod	ECFP,expressing
R3220	T5646	T5647	amod	bright,field
R3221	T5647	T5645	pobj	field,under
R3222	T5648	T5644	prep	with,taken
R3223	T5649	T5650	det	no,epifluorescence
R3224	T5650	T5648	pobj	epifluorescence,with
R3225	T5651	T5642	punct	.,image
R3226	T5653	T5654	punct	(,f
R3227	T5654	T5655	meta	f,image
R3228	T5656	T5654	punct	),f
R3229	T5657	T5658	amod	dark,field
R323	T502	T500	amod	expressing,cells
R3230	T5658	T5655	compound	field,image
R3231	T5659	T5655	acl	taken,image
R3232	T5660	T5659	prep	through,taken
R3233	T5661	T5662	det	an,filter
R3234	T5662	T5660	pobj	filter,through
R3235	T5663	T5662	compound	EYFP,filter
R3236	T5664	T5655	punct	.,image
R3237	T5666	T5667	punct	(,g
R3238	T5667	T5668	meta	g,image
R3239	T5669	T5667	punct	),g
R324	T503	T501	cc	and,ECFP
R3240	T5670	T5668	punct	", ",image
R3241	T5671	T5672	amod	dark,field
R3242	T5672	T5668	compound	field,image
R3243	T5673	T5668	acl	taken,image
R3244	T5674	T5673	prep	through,taken
R3245	T5675	T5676	det	an,filter
R3246	T5676	T5674	pobj	filter,through
R3247	T5677	T5676	compound	ECFP,filter
R3248	T5678	T5668	punct	.,image
R3249	T5680	T5681	punct	(,h
R325	T504	T501	conj	EYFP,ECFP
R3250	T5681	T5682	meta	h,exposure
R3251	T5683	T5681	punct	),h
R3252	T5684	T5682	amod	double,exposure
R3253	T5685	T5682	acl	acquired,exposure
R3254	T5686	T5685	prep	by,acquired
R3255	T5687	T5688	advmod	consecutively,using
R3256	T5688	T5686	pcomp	using,by
R3257	T5689	T5690	nmod	ECFP,filters
R3258	T5690	T5688	dobj	filters,using
R3259	T5691	T5689	cc	and,ECFP
R326	T505	T502	punct	-,expressing
R3260	T5692	T5689	conj	EYFP,ECFP
R3261	T5693	T5682	punct	.,exposure
R3262	T5695	T5696	punct	(,i
R3263	T5696	T5697	meta	i,mice
R3264	T5698	T5696	punct	-,i
R3265	T5699	T5696	dep	k,i
R3266	T5700	T5696	punct	),i
R3267	T5701	T5697	nummod	two,mice
R3268	T5702	T5703	nummod	three,week
R3269	T5703	T5704	npadvmod	week,old
R327	T506	T500	amod	transgenic,cells
R3270	T5704	T5697	amod	old,mice
R3271	T5705	T5697	amod	transgenic,mice
R3272	T5706	T5697	appos	each,mice
R3273	T5707	T5708	advmod	exclusively,expressing
R3274	T5708	T5706	acl	expressing,each
R3275	T5709	T5710	preconj	either,ECFP
R3276	T5710	T5712	nmod	ECFP,reporter
R3277	T5711	T5710	det	the,ECFP
R3278	T5712	T5708	dobj	reporter,expressing
R3279	T5713	T5710	cc	or,ECFP
R328	T507	T500	compound	ES,cells
R3280	T5714	T5710	conj	EYFP,ECFP
R3281	T5715	T5697	punct	.,mice
R3282	T5717	T5718	det	All,images
R3283	T5718	T5719	nsubj	images,are
R3284	T5720	T5718	acl	shown,images
R3285	T5721	T5722	amod	double,exposures
R3286	T5722	T5719	attr	exposures,are
R3287	T5723	T5722	acl	taken,exposures
R3288	T5724	T5723	prep	by,taken
R3289	T5725	T5726	advmod	consecutively,using
R329	T509	T500	cc	and,cells
R3290	T5726	T5724	pcomp	using,by
R3291	T5727	T5726	dobj	ECFP,using
R3292	T5728	T5727	cc	and,ECFP
R3293	T5729	T5728	advmod	then,and
R3294	T5730	T5731	det	the,EYFP
R3295	T5731	T5732	compound	EYFP,filters
R3296	T5732	T5727	conj	filters,ECFP
R3297	T5733	T5726	prep	under,using
R3298	T5734	T5733	pobj	epifluorescence,under
R3299	T5735	T5719	punct	.,are
R33	T189	T187	pobj	array,in
R330	T510	T500	conj	mice,cells
R3300	T5737	T5738	punct	(,i
R3301	T5738	T5739	meta	i,mouse
R3302	T5740	T5738	punct	),i
R3303	T5741	T5742	nmod	CK6,ECFP
R3304	T5742	T5739	nmod	ECFP,mouse
R3305	T5743	T5742	punct	/,ECFP
R3306	T5744	T5742	punct	(,ECFP
R3307	T5745	T5742	appos	TG,ECFP
R3308	T5746	T5745	punct	/,TG
R3309	T5747	T5745	punct	+,TG
R331	T511	T512	dep	that,generated
R3310	T5748	T5742	punct	),ECFP
R3311	T5749	T5739	amod	transgenic,mouse
R3312	T5750	T5739	parataxis	bottom,mouse
R3313	T5751	T5739	punct	", ",mouse
R3314	T5752	T5753	nmod	YC5,EYFP
R3315	T5753	T5755	nmod	EYFP,mouse
R3316	T5754	T5753	punct	/,EYFP
R3317	T5755	T5739	appos	mouse,mouse
R3318	T5756	T5753	punct	(,EYFP
R3319	T5757	T5753	appos	TG,EYFP
R332	T512	T500	relcl	generated,cells
R3320	T5758	T5757	punct	/,TG
R3321	T5759	T5757	punct	+,TG
R3322	T5760	T5753	punct	),EYFP
R3323	T5761	T5755	amod	transgenic,mouse
R3324	T5762	T5755	parataxis	bottom,mouse
R3325	T5763	T5739	punct	.,mouse
R3326	T5765	T5766	punct	(,j
R3327	T5766	T5767	meta	j,mouse
R3328	T5768	T5766	punct	),j
R3329	T5769	T5770	nmod	CK6,ECFP
R333	T513	T512	nsubj	we,generated
R3330	T5770	T5767	nmod	ECFP,mouse
R3331	T5771	T5770	punct	/,ECFP
R3332	T5772	T5770	punct	(,ECFP
R3333	T5773	T5770	appos	TG,ECFP
R3334	T5774	T5773	punct	/,TG
R3335	T5775	T5773	punct	+,TG
R3336	T5776	T5770	punct	),ECFP
R3337	T5777	T5767	amod	transgenic,mouse
R3338	T5778	T5767	parataxis	left,mouse
R3339	T5779	T5767	punct	", ",mouse
R334	T514	T512	aux	have,generated
R3340	T5780	T5781	nmod	YC5,EYFP
R3341	T5781	T5783	nmod	EYFP,mouse
R3342	T5782	T5781	punct	/,EYFP
R3343	T5783	T5767	appos	mouse,mouse
R3344	T5784	T5781	punct	(,EYFP
R3345	T5785	T5781	appos	TG,EYFP
R3346	T5786	T5785	punct	/,TG
R3347	T5787	T5785	punct	+,TG
R3348	T5788	T5781	punct	),EYFP
R3349	T5789	T5783	amod	transgenic,mouse
R335	T515	T508	dobj	sources,provide
R3350	T5790	T5783	parataxis	right,mouse
R3351	T5791	T5767	punct	.,mouse
R3352	T5793	T5794	punct	(,k
R3353	T5794	T5795	meta	k,view
R3354	T5796	T5794	punct	),k
R3355	T5797	T5795	amod	higher,view
R3356	T5798	T5795	compound	maginification,view
R3357	T5799	T5795	prep	of,view
R3358	T5800	T5801	det	the,tails
R3359	T5801	T5799	pobj	tails,of
R336	T516	T515	prep	of,sources
R3360	T5802	T5801	prep	of,tails
R3361	T5803	T5804	det	the,mice
R3362	T5804	T5802	pobj	mice,of
R3363	T5805	T5804	prep	in,mice
R3364	T5806	T5805	pobj	i,in
R3365	T5807	T5806	cc	and,i
R3366	T5808	T5806	conj	j,i
R3367	T5809	T5795	punct	.,view
R337	T517	T516	pobj	cells,of
R3371	T5891	T5892	amod	Dual,visualization
R3372	T5893	T5892	amod	non-invasive,visualization
R3373	T5894	T5892	compound	reporter,visualization
R3374	T5895	T5892	prep	in,visualization
R3375	T5896	T5897	amod	chimeric,embryos
R3376	T5897	T5895	pobj	embryos,in
R3377	T5898	T5897	acl	generated,embryos
R3378	T5899	T5898	prep	by,generated
R3379	T5900	T5901	amod	tetraploid,aggregation
R338	T518	T517	cc	and,cells
R3380	T5901	T5899	pobj	aggregation,by
R3381	T5902	T5900	cc	<,tetraploid
R3382	T5903	T5902	punct	-,<
R3383	T5904	T5902	punct	>,<
R3384	T5905	T5900	conj	diploid,tetraploid
R3385	T5906	T5892	punct	.,visualization
R3386	T5908	T5909	prep	In,is
R3387	T5910	T5911	det	all,panels
R3388	T5911	T5908	pobj	panels,In
R3389	T5912	T5913	det	the,compartment
R339	T519	T517	conj	tissues,cells
R3390	T5913	T5909	nsubj	compartment,is
R3391	T5914	T5913	amod	tetraploid,compartment
R3392	T5915	T5916	amod	yellow,green
R3393	T5916	T5918	amod	green,fluorescent
R3394	T5917	T5916	punct	/,green
R3395	T5918	T5919	amod	fluorescent,EYFP+
R3396	T5919	T5909	acomp	EYFP+,is
R3397	T5920	T5919	punct	(,EYFP+
R3398	T5921	T5909	punct	),is
R3399	T5922	T5909	cc	and,is
R34	T190	T189	prep	of,array
R340	T520	T508	prep	for,provide
R3400	T5923	T5924	det	the,diploid
R3401	T5924	T5925	nsubj	diploid,is
R3402	T5925	T5909	conj	is,is
R3403	T5926	T5927	amod	blue,cyan
R3404	T5927	T5929	amod	cyan,fluorescent
R3405	T5928	T5927	punct	/,cyan
R3406	T5929	T5930	amod	fluorescent,ECFP+
R3407	T5930	T5925	acomp	ECFP+,is
R3408	T5931	T5930	punct	(,ECFP+
R3409	T5932	T5909	punct	),is
R341	T521	T522	amod	combinatorial,experiments
R3410	T5933	T5909	punct	.,is
R3411	T5935	T5936	punct	(,a
R3412	T5936	T5937	meta	a,representation
R3413	T5938	T5936	punct	),a
R3414	T5939	T5937	amod	schematic,representation
R3415	T5940	T5937	prep	of,representation
R3416	T5941	T5942	det	the,procedure
R3417	T5942	T5940	pobj	procedure,of
R3418	T5943	T5942	amod	tetraploid,procedure
R3419	T5944	T5937	punct	.,representation
R342	T522	T520	pobj	experiments,for
R3420	T5947	T5946	prep	of,Electrofusion
R3421	T5948	T5949	compound	E1.5,embryos
R3422	T5949	T5947	pobj	embryos,of
R3423	T5950	T5951	aux	to,render
R3424	T5951	T5946	advcl	render,Electrofusion
R3425	T5952	T5953	det	the,blastomeres
R3426	T5953	T5951	dobj	blastomeres,render
R3427	T5954	T5951	oprd	tetraploid,render
R3428	T5955	T5946	punct	", ",Electrofusion
R3429	T5956	T5957	advmod	in,vitro
R343	T523	T522	punct	", ",experiments
R3430	T5957	T5958	amod	vitro,culture
R3431	T5958	T5946	conj	culture,Electrofusion
R3432	T5959	T5958	punct	", ",culture
R3433	T5960	T5958	cc	and,culture
R3434	T5961	T5962	amod	subsequent,schemes
R3435	T5962	T5958	conj	schemes,culture
R3436	T5963	T5962	compound	aggregation,schemes
R3437	T5964	T5962	acl	using,schemes
R3438	T5965	T5966	amod	double,tagged
R3439	T5966	T5968	amod	tagged,compartments
R344	T524	T525	amod	double,tagged
R3440	T5967	T5966	punct	-,tagged
R3441	T5968	T5964	dobj	compartments,using
R3442	T5969	T5962	punct	.,schemes
R3443	T5971	T5972	amod	Sandwich,aggregations
R3444	T5972	T5973	nsubjpass	aggregations,made
R3445	T5974	T5973	auxpass	were,made
R3446	T5975	T5973	advcl	using,made
R3447	T5976	T5977	preconj	either,morulae
R3448	T5977	T5975	dobj	morulae,using
R3449	T5978	T5977	amod	ECFP+,morulae
R345	T525	T522	amod	tagged,experiments
R3450	T5979	T5977	punct	(,morulae
R3451	T5980	T5977	amod	diploid,morulae
R3452	T5981	T5977	punct	),morulae
R3453	T5982	T5977	cc	or,morulae
R3454	T5983	T5984	compound	ES,cells
R3455	T5984	T5977	conj	cells,morulae
R3456	T5985	T5973	punct	.,made
R3457	T5987	T5988	punct	(,b
R3458	T5988	T5989	meta	b,view
R3459	T5990	T5988	punct	-,b
R346	T526	T525	punct	-,tagged
R3460	T5991	T5988	dep	d,b
R3461	T5992	T5988	punct	),b
R3462	T5993	T5989	amod	anterior,view
R3463	T5994	T5989	prep	of,view
R3464	T5995	T5996	det	an,chimera
R3465	T5996	T5994	pobj	chimera,of
R3466	T5997	T5996	nmod	E7.5,chimera
R3467	T5998	T5999	amod	double,tagged
R3468	T5999	T5996	amod	tagged,chimera
R3469	T6000	T5999	punct	-,tagged
R347	T527	T522	compound	recombination,experiments
R3470	T6001	T5996	amod	polarized,chimera
R3471	T6002	T5996	acl	dissected,chimera
R3472	T6003	T6002	advcl	free,dissected
R3473	T6004	T6003	prep	of,free
R3474	T6005	T6006	poss	its,deciduum
R3475	T6006	T6004	pobj	deciduum,of
R3476	T6007	T6002	cc	and,dissected
R3477	T6008	T6009	advmod	therefore,missing
R3478	T6009	T6002	conj	missing,dissected
R3479	T6010	T6011	amod	primary,cells
R348	T528	T522	punct	", ",experiments
R3480	T6011	T6009	dobj	cells,missing
R3481	T6012	T6011	amod	giant,cells
R3482	T6013	T6011	cc	and,cells
R3483	T6014	T6015	amod	parietal,endoderm
R3484	T6015	T6011	conj	endoderm,cells
R3485	T6016	T5989	punct	.,view
R3486	T6018	T6019	advmod	Here,are
R3487	T6020	T6021	det	the,epiblast
R3488	T6021	T6019	nsubj	epiblast,are
R3489	T6022	T6023	punct	(,half
R349	T529	T522	conj	chimeras,experiments
R3490	T6023	T6021	parataxis	half,epiblast
R3491	T6024	T6023	amod	lower,half
R3492	T6025	T6023	prep	of,half
R3493	T6026	T6025	pobj	embryo,of
R3494	T6027	T6023	punct	),half
R3495	T6028	T6021	cc	and,epiblast
R3496	T6029	T6030	poss	its,derivatives
R3497	T6030	T6021	conj	derivatives,epiblast
R3498	T6031	T6019	acomp	ECFP+,are
R3499	T6032	T6033	mark	whereas,are
R35	T191	T192	amod	different,organisms
R350	T530	T529	cc	or,chimeras
R3500	T6033	T6019	advcl	are,are
R3501	T6034	T6035	det	the,ectoderm
R3502	T6035	T6033	nsubj	ectoderm,are
R3503	T6036	T6035	amod	extraembryonic,ectoderm
R3504	T6037	T6035	punct	", ",ectoderm
R3505	T6038	T6039	det	the,endoderm
R3506	T6039	T6035	conj	endoderm,ectoderm
R3507	T6040	T6039	amod	visceral,endoderm
R3508	T6041	T6039	cc	and,endoderm
R3509	T6042	T6039	conj	trophoblast,endoderm
R351	T531	T529	conj	transplantations,chimeras
R3510	T6043	T6033	acomp	EYFP+,are
R3511	T6044	T6045	punct	(,half
R3512	T6045	T6033	parataxis	half,are
R3513	T6046	T6045	amod	upper,half
R3514	T6047	T6045	cc	or,half
R3515	T6048	T6045	conj	embryo,half
R3516	T6049	T6045	punct	),half
R3517	T6050	T6019	punct	.,are
R3518	T6052	T6053	punct	(,b
R3519	T6053	T6054	meta	b,image
R352	T532	T508	punct	.,provide
R3520	T6055	T6053	punct	),b
R3521	T6056	T6057	amod	dark,field
R3522	T6057	T6054	compound	field,image
R3523	T6058	T6054	acl	taken,image
R3524	T6059	T6058	prep	through,taken
R3525	T6060	T6061	det	an,filter
R3526	T6061	T6059	pobj	filter,through
R3527	T6062	T6061	compound	EYFP,filter
R3528	T6063	T6054	punct	.,image
R3529	T6065	T6066	punct	(,c
R353	T157	T158	amod	Embryonic,cells
R3530	T6066	T6067	meta	c,image
R3531	T6068	T6066	punct	),c
R3532	T6069	T6070	nmod	dark,field
R3533	T6070	T6067	compound	field,image
R3534	T6071	T6067	acl	taken,image
R3535	T6072	T6071	prep	through,taken
R3536	T6073	T6074	det	an,filter
R3537	T6074	T6072	pobj	filter,through
R3538	T6075	T6074	compound	ECFP,filter
R3539	T6076	T6067	punct	.,image
R354	T646	T647	det	The,ease
R3540	T6078	T6079	punct	(,d
R3541	T6079	T6080	meta	d,image
R3542	T6081	T6079	punct	),d
R3543	T6082	T6083	amod	dark,field
R3544	T6083	T6080	nmod	field,image
R3545	T6084	T6085	amod	double,exposure
R3546	T6085	T6080	compound	exposure,image
R3547	T6086	T6080	acl	acquired,image
R3548	T6087	T6086	prep	by,acquired
R3549	T6088	T6089	advmod	consecutively,using
R355	T647	T648	nsubj	ease,establish
R3550	T6089	T6087	pcomp	using,by
R3551	T6090	T6091	nmod	ECFP,filters
R3552	T6091	T6089	dobj	filters,using
R3553	T6092	T6090	cc	and,ECFP
R3554	T6093	T6090	conj	EYFP,ECFP
R3555	T6094	T6080	punct	.,image
R3556	T6096	T6097	punct	(,e
R3557	T6097	T6098	meta	e,exposure
R3558	T6099	T6097	punct	),e
R3559	T6100	T6101	amod	dark,field
R356	T649	T647	prep	of,ease
R3560	T6101	T6098	nmod	field,exposure
R3561	T6102	T6103	amod	double,filter
R3562	T6103	T6098	compound	filter,exposure
R3563	T6104	T6098	prep	of,exposure
R3564	T6105	T6106	det	an,chimera
R3565	T6106	T6104	pobj	chimera,of
R3566	T6107	T6106	compound	E9,chimera
R3567	T6108	T6106	acl	dissected,chimera
R3568	T6109	T6108	advcl	free,dissected
R3569	T6110	T6109	prep	of,free
R357	T650	T649	pcomp	manipulating,of
R3570	T6111	T6112	det	all,membranes
R3571	T6112	T6110	pobj	membranes,of
R3572	T6113	T6112	amod	extraembryonic,membranes
R3573	T6114	T6112	prep	except,membranes
R3574	T6115	T6114	prep	for,except
R3575	T6116	T6117	det	a,piece
R3576	T6117	T6115	pobj	piece,for
R3577	T6118	T6117	amod	small,piece
R3578	T6119	T6117	prep	of,piece
R3579	T6120	T6121	compound	yolk,sac
R358	T651	T652	poss	its,genome
R3580	T6121	T6119	pobj	sac,of
R3581	T6122	T6123	punct	(,left
R3582	T6123	T6098	parataxis	left,exposure
R3583	T6124	T6123	amod	lower,left
R3584	T6125	T6123	punct	),left
R3585	T6126	T6098	punct	.,exposure
R3586	T6128	T6129	det	The,embryo
R3587	T6129	T6130	nsubj	embryo,is
R3588	T6131	T6129	appos	itself,embryo
R3589	T6132	T6130	acomp	ECFP+,is
R359	T652	T650	dobj	genome,manipulating
R3590	T6133	T6134	mark	whereas,ECFP+
R3591	T6134	T6130	advcl	ECFP+,is
R3592	T6135	T6136	det	the,sac
R3593	T6136	T6134	nsubj	sac,ECFP+
R3594	T6137	T6136	compound	yolk,sac
R3595	T6138	T6134	preconj	both,ECFP+
R3596	T6139	T6134	cc	and,ECFP+
R3597	T6140	T6134	conj	EYFP+,ECFP+
R3598	T6141	T6134	prep	in,ECFP+
R3599	T6142	T6143	det	the,mesoderm
R36	T192	T190	pobj	organisms,of
R360	T653	T648	aux	has,establish
R3600	T6143	T6141	pobj	mesoderm,in
R3601	T6144	T6143	cc	and,mesoderm
R3602	T6145	T6143	conj	endoderm,mesoderm
R3603	T6146	T6134	advmod	respectively,ECFP+
R3604	T6147	T6130	punct	.,is
R3605	T6149	T6150	det	A,number
R3606	T6150	T6152	nsubjpass	number,observed
R3607	T6151	T6150	amod	small,number
R3608	T6153	T6150	prep	of,number
R3609	T6154	T6155	amod	EYFP+,cells
R361	T654	T648	aux	helped,establish
R3610	T6155	T6153	pobj	cells,of
R3611	T6156	T6152	auxpass	are,observed
R3612	T6157	T6152	advmod	also,observed
R3613	T6158	T6152	prep	in,observed
R3614	T6159	T6160	det	the,midline
R3615	T6160	T6158	pobj	midline,in
R3616	T6161	T6160	amod	ventral,midline
R3617	T6162	T6160	prep	of,midline
R3618	T6163	T6164	det	the,embryo
R3619	T6164	T6162	pobj	embryo,of
R362	T655	T656	det	the,mouse
R3620	T6165	T6166	punct	(,arrowhead
R3621	T6166	T6164	parataxis	arrowhead,embryo
R3622	T6167	T6166	punct	),arrowhead
R3623	T6168	T6152	punct	.,observed
R3624	T6170	T6171	det	This,observation
R3625	T6171	T6172	nsubjpass	observation,noted
R3626	T6173	T6172	aux	has,noted
R3627	T6174	T6172	auxpass	been,noted
R3628	T6175	T6172	advmod	previously,noted
R3629	T6176	T6172	prep	in,noted
R363	T656	T648	dobj	mouse,establish
R3630	T6177	T6178	amod	tetraploid,chimeras
R3631	T6178	T6176	pobj	chimeras,in
R3632	T6179	T6172	punct	", ",noted
R3633	T6180	T6172	cc	and,noted
R3634	T6181	T6182	auxpass	is,presumed
R3635	T6182	T6172	conj	presumed,noted
R3636	T6183	T6184	aux	to,represent
R3637	T6184	T6182	xcomp	represent,presumed
R3638	T6185	T6186	det	a,population
R3639	T6186	T6184	dobj	population,represent
R364	T657	T648	prep	as,establish
R3640	T6187	T6186	amod	small,population
R3641	T6188	T6186	prep	of,population
R3642	T6189	T6190	amod	visceral,cells
R3643	T6190	T6188	pobj	cells,of
R3644	T6191	T6190	compound	endoderm,cells
R3645	T6192	T6193	dep	that,fail
R3646	T6193	T6190	relcl	fail,cells
R3647	T6194	T6195	aux	to,displaced
R3648	T6195	T6193	xcomp	displaced,fail
R3649	T6196	T6195	auxpass	be,displaced
R365	T658	T659	det	the,organism
R3650	T6197	T6195	agent	by,displaced
R3651	T6198	T6199	det	the,endoderm
R3652	T6199	T6197	pobj	endoderm,by
R3653	T6200	T6199	amod	definitive,endoderm
R3654	T6201	T6195	prep	at,displaced
R3655	T6202	T6203	amod	earlier,stages
R3656	T6203	T6201	pobj	stages,at
R3657	T6204	T6172	punct	.,noted
R3658	T6206	T6207	punct	(,f
R3659	T6207	T6208	meta	f,exposure
R366	T659	T657	pobj	organism,as
R3660	T6209	T6207	punct	),f
R3661	T6210	T6211	amod	dark,field
R3662	T6211	T6208	nmod	field,exposure
R3663	T6212	T6213	amod	double,filter
R3664	T6213	T6208	compound	filter,exposure
R3665	T6214	T6208	prep	of,exposure
R3666	T6215	T6216	det	an,chimera
R3667	T6216	T6214	pobj	chimera,of
R3668	T6217	T6216	compound	E10,chimera
R3669	T6218	T6208	punct	.,exposure
R367	T660	T659	amod	premier,organism
R3670	T6220	T6221	det	The,embryo
R3671	T6221	T6222	nsubj	embryo,is
R3672	T6223	T6224	advmod	exclusively,ECFP+
R3673	T6224	T6222	acomp	ECFP+,is
R3674	T6225	T6226	mark	whereas,is
R3675	T6226	T6222	advcl	is,is
R3676	T6227	T6228	poss	its,placenta
R3677	T6228	T6226	nsubj	placenta,is
R3678	T6229	T6230	punct	(,to
R3679	T6230	T6228	parataxis	to,placenta
R368	T661	T659	compound	model,organism
R3680	T6231	T6232	det	the,left
R3681	T6232	T6230	pobj	left,to
R3682	T6233	T6232	prep	of,left
R3683	T6234	T6235	det	the,embryo
R3684	T6235	T6233	pobj	embryo,of
R3685	T6236	T6230	punct	),to
R3686	T6237	T6238	advmod	predominantly,EYFP+
R3687	T6238	T6226	acomp	EYFP+,is
R3688	T6239	T6222	punct	.,is
R3689	T6241	T6242	det	The,layer
R369	T662	T659	prep	for,organism
R3690	T6242	T6245	nsubj	layer,comprises
R3691	T6243	T6242	compound	labyrinthine,layer
R3692	T6244	T6242	compound	trophoblast,layer
R3693	T6246	T6242	prep	of,layer
R3694	T6247	T6248	det	the,placenta
R3695	T6248	T6246	pobj	placenta,of
R3696	T6249	T6250	preconj	both,ECFP+
R3697	T6250	T6251	amod	ECFP+,cells
R3698	T6251	T6245	dobj	cells,comprises
R3699	T6252	T6250	cc	and,ECFP+
R37	T193	T183	punct	.,revolutionized
R370	T663	T664	amod	mammalian,studies
R3700	T6253	T6250	conj	EYFP+,ECFP+
R3701	T6254	T6245	punct	.,comprises
R3707	T6343	T6344	amod	Dual,visualization
R3708	T6345	T6344	amod	non-invasive,visualization
R3709	T6346	T6344	compound	reporter,visualization
R371	T664	T662	pobj	studies,for
R3710	T6347	T6344	prep	in,visualization
R3711	T6348	T6349	det	the,organs
R3712	T6349	T6347	pobj	organs,in
R3713	T6350	T6349	prep	of,organs
R3714	T6351	T6352	amod	adult,chimeras
R3715	T6352	T6350	pobj	chimeras,of
R3716	T6353	T6344	punct	.,visualization
R3717	T6355	T6356	det	All,images
R3718	T6356	T6357	nsubj	images,depict
R3719	T6358	T6359	amod	dark,field
R372	T665	T664	amod	genetic,studies
R3720	T6359	T6360	nmod	field,exposures
R3721	T6360	T6357	dobj	exposures,depict
R3722	T6361	T6360	amod	double,exposures
R3723	T6362	T6360	acl	acquired,exposures
R3724	T6363	T6362	prep	by,acquired
R3725	T6364	T6365	advmod	consecutively,using
R3726	T6365	T6363	pcomp	using,by
R3727	T6366	T6367	nmod	ECFP,filters
R3728	T6367	T6365	dobj	filters,using
R3729	T6368	T6366	cc	and,ECFP
R373	T666	T648	punct	.,establish
R3730	T6369	T6366	conj	EYFP,ECFP
R3731	T6370	T6365	prep	under,using
R3732	T6371	T6372	amod	dark,field
R3733	T6372	T6373	compound	field,epifluorescence
R3734	T6373	T6370	pobj	epifluorescence,under
R3735	T6374	T6357	punct	.,depict
R3736	T6376	T6377	nsubjpass	Details,schematized
R3737	T6378	T6376	prep	of,Details
R3738	T6379	T6378	pobj	aggregates,of
R3739	T6380	T6377	auxpass	are,schematized
R374	T668	T669	preconj	Both,directed
R3740	T6381	T6377	prep	at,schematized
R3741	T6382	T6383	det	the,right
R3742	T6383	T6381	pobj	right,at
R3743	T6384	T6383	amod	top,right
R3744	T6385	T6383	prep	of,right
R3745	T6386	T6387	det	each,panel
R3746	T6387	T6385	pobj	panel,of
R3747	T6388	T6377	punct	.,schematized
R3748	T6390	T6391	punct	(,a
R3749	T6391	T6392	meta	a,heart
R375	T669	T670	amod	directed,approaches
R3750	T6393	T6391	punct	-,a
R3751	T6394	T6391	dep	c,a
R3752	T6395	T6391	punct	),a
R3753	T6396	T6392	cc	and,heart
R3754	T6397	T6392	conj	pancreas,heart
R3755	T6398	T6392	prep	from,heart
R3756	T6399	T6400	amod	double,tagged
R3757	T6400	T6402	amod	tagged,chimeras
R3758	T6401	T6400	punct	-,tagged
R3759	T6402	T6398	pobj	chimeras,from
R376	T670	T674	nsubj	approaches,become
R3760	T6403	T6404	amod	double,compartment
R3761	T6404	T6402	nmod	compartment,chimeras
R3762	T6405	T6404	punct	-,compartment
R3763	T6406	T6402	amod	adult,chimeras
R3764	T6407	T6392	punct	.,heart
R3765	T6409	T6410	punct	(,a
R3766	T6410	T6411	meta	a,view
R3767	T6412	T6410	punct	),a
R3768	T6413	T6411	prep	of,view
R3769	T6414	T6415	det	the,surface
R377	T671	T669	cc	and,directed
R3770	T6415	T6413	pobj	surface,of
R3771	T6416	T6415	prep	of,surface
R3772	T6417	T6418	det	an,heart
R3773	T6418	T6416	pobj	heart,of
R3774	T6419	T6418	amod	adult,heart
R3775	T6420	T6411	punct	.,view
R3776	T6422	T6423	punct	(,b
R3777	T6423	T6424	meta	b,view
R3778	T6425	T6423	punct	),b
R3779	T6426	T6427	compound	close,up
R378	T672	T669	conj	random,directed
R3780	T6427	T6424	compound	up,view
R3781	T6428	T6427	punct	-,up
R3782	T6429	T6424	compound	surface,view
R3783	T6430	T6424	prep	of,view
R3784	T6431	T6430	pobj	ventricle,of
R3785	T6432	T6431	prep	of,ventricle
R3786	T6433	T6434	det	a,heart
R3787	T6434	T6432	pobj	heart,of
R3788	T6435	T6434	amod	chimeric,heart
R3789	T6436	T6424	acl	generated,view
R379	T673	T670	compound	mutagenesis,approaches
R3790	T6437	T6436	prep	by,generated
R3791	T6438	T6437	pobj	aggregation,by
R3792	T6439	T6438	prep	of,aggregation
R3793	T6440	T6441	nummod	two,morulae
R3794	T6441	T6439	pobj	morulae,of
R3795	T6442	T6441	amod	diploid,morulae
R3796	T6443	T6441	punct	", ",morulae
R3797	T6444	T6441	appos	one,morulae
R3798	T6445	T6444	amod	hemizygous,one
R3799	T6446	T6445	prep	for,hemizygous
R38	T195	T196	prep	In,gained
R380	T675	T670	punct	", ",approaches
R3800	T6447	T6448	det	the,transgene
R3801	T6448	T6446	pobj	transgene,for
R3802	T6449	T6450	nmod	CK6,ECFP
R3803	T6450	T6448	nmod	ECFP,transgene
R3804	T6451	T6450	punct	/,ECFP
R3805	T6452	T6448	punct	(,transgene
R3806	T6453	T6448	amod	ECFP+,transgene
R3807	T6454	T6448	punct	),transgene
R3808	T6455	T6444	cc	and,one
R3809	T6456	T6457	det	the,other
R381	T676	T670	prep	including,approaches
R3810	T6457	T6444	conj	other,one
R3811	T6458	T6457	amod	hemizygous,other
R3812	T6459	T6458	prep	for,hemizygous
R3813	T6460	T6461	det	the,transgene
R3814	T6461	T6459	pobj	transgene,for
R3815	T6462	T6463	nmod	YC5,EYFP
R3816	T6463	T6461	nmod	EYFP,transgene
R3817	T6464	T6463	punct	/,EYFP
R3818	T6465	T6461	punct	(,transgene
R3819	T6466	T6461	amod	EYFP+,transgene
R382	T677	T678	det	the,technologies
R3820	T6467	T6461	punct	),transgene
R3821	T6468	T6424	punct	.,view
R3822	T6470	T6471	det	The,regions
R3823	T6471	T6472	nsubj	regions,are
R3824	T6473	T6471	prep	of,regions
R3825	T6474	T6475	amod	yellow,green
R3826	T6475	T6477	amod	green,fluorescence
R3827	T6476	T6475	punct	/,green
R3828	T6477	T6473	pobj	fluorescence,of
R3829	T6478	T6475	cc	and,green
R383	T678	T676	pobj	technologies,including
R3830	T6479	T6475	conj	cyan,green
R3831	T6480	T6472	advmod	clearly,are
R3832	T6481	T6482	advmod	mutually,exclusive
R3833	T6482	T6472	acomp	exclusive,are
R3834	T6483	T6472	punct	.,are
R3835	T6485	T6486	det	The,striations
R3836	T6486	T6487	nsubj	striations,represent
R3837	T6488	T6486	prep	in,striations
R3838	T6489	T6490	det	the,fields
R3839	T6490	T6488	pobj	fields,in
R384	T679	T678	prep	of,technologies
R3840	T6491	T6490	prep	of,fields
R3841	T6492	T6491	pobj	fluorescence,of
R3842	T6493	T6494	det	the,zones
R3843	T6494	T6487	dobj	zones,represent
R3844	T6495	T6494	amod	proliferative,zones
R3845	T6496	T6494	amod	present,zones
R3846	T6497	T6496	prep	within,present
R3847	T6498	T6499	det	the,ventricle
R3848	T6499	T6497	pobj	ventricle,within
R3849	T6500	T6487	punct	.,represent
R385	T680	T679	pobj	transgenesis,of
R3850	T6502	T6503	punct	(,c
R3851	T6503	T6504	meta	c,pancreas
R3852	T6505	T6503	punct	),c
R3853	T6506	T6504	amod	chimeric,pancreas
R3854	T6507	T6504	acl	generated,pancreas
R3855	T6508	T6507	prep	by,generated
R3856	T6509	T6508	pobj	aggregation,by
R3857	T6510	T6509	prep	of,aggregation
R3858	T6511	T6512	det	a,morula
R3859	T6512	T6510	pobj	morula,of
R386	T681	T680	cc	and,transgenesis
R3860	T6513	T6512	amod	hemizygous,morula
R3861	T6514	T6507	prep	for,generated
R3862	T6515	T6516	det	the,transgene
R3863	T6516	T6514	pobj	transgene,for
R3864	T6517	T6518	compound	YC5,EYFP
R3865	T6518	T6516	compound	EYFP,transgene
R3866	T6519	T6518	punct	/,EYFP
R3867	T6520	T6507	prep	with,generated
R3868	T6521	T6522	compound	CK6,ECFP
R3869	T6522	T6524	compound	ECFP,cells
R387	T682	T683	compound	gene,targeting
R3870	T6523	T6522	punct	/,ECFP
R3871	T6524	T6520	pobj	cells,with
R3872	T6525	T6524	compound	ES,cells
R3873	T6526	T6504	punct	.,pancreas
R3874	T6528	T6529	det	The,fields
R3875	T6529	T6531	nsubj	fields,represent
R3876	T6530	T6529	amod	restricted,fields
R3877	T6532	T6529	prep	of,fields
R3878	T6533	T6532	pobj	fluorescence,of
R3879	T6534	T6535	det	the,zones
R388	T683	T680	conj	targeting,transgenesis
R3880	T6535	T6531	dobj	zones,represent
R3881	T6536	T6535	amod	proliferative,zones
R3882	T6537	T6535	amod	present,zones
R3883	T6538	T6537	prep	within,present
R3884	T6539	T6540	det	the,pancreas
R3885	T6540	T6538	pobj	pancreas,within
R3886	T6541	T6531	punct	.,represent
R3887	T6543	T6544	punct	(,d
R3888	T6544	T6545	meta	d,liver
R3889	T6546	T6544	punct	),d
R389	T684	T683	prep	in,targeting
R3890	T6547	T6545	prep	from,liver
R3891	T6548	T6549	det	a,chimera
R3892	T6549	T6547	pobj	chimera,from
R3893	T6550	T6551	amod	double,tagged
R3894	T6551	T6549	amod	tagged,chimera
R3895	T6552	T6551	punct	-,tagged
R3896	T6553	T6554	amod	triple,compartment
R3897	T6554	T6549	nmod	compartment,chimera
R3898	T6555	T6554	punct	-,compartment
R3899	T6556	T6549	amod	adult,chimera
R39	T197	T198	amod	recent,years
R390	T685	T686	compound	ES,cells
R3900	T6557	T6545	punct	.,liver
R3901	T6559	T6560	det	A,morula
R3902	T6560	T6562	nsubjpass	morula,aggregated
R3903	T6561	T6560	amod	non-fluorescent,morula
R3904	T6563	T6562	auxpass	was,aggregated
R3905	T6564	T6562	prep	with,aggregated
R3906	T6565	T6566	nummod	two,clumps
R3907	T6566	T6564	pobj	clumps,with
R3908	T6567	T6566	prep	of,clumps
R3909	T6568	T6569	compound	ES,cells
R391	T686	T684	pobj	cells,in
R3910	T6569	T6567	pobj	cells,of
R3911	T6570	T6566	punct	", ",clumps
R3912	T6571	T6566	appos	one,clumps
R3913	T6572	T6571	acl	carrying,one
R3914	T6573	T6574	det	the,transgene
R3915	T6574	T6572	dobj	transgene,carrying
R3916	T6575	T6576	compound	CK6,ECFP
R3917	T6576	T6574	compound	ECFP,transgene
R3918	T6577	T6576	punct	/,ECFP
R3919	T6578	T6574	punct	(,transgene
R392	T687	T674	punct	", ",become
R3920	T6579	T6574	amod	ECFP+,transgene
R3921	T6580	T6571	punct	),one
R3922	T6581	T6571	cc	and,one
R3923	T6582	T6583	det	the,other
R3924	T6583	T6571	conj	other,one
R3925	T6584	T6583	acl	carrying,other
R3926	T6585	T6586	det	the,transgene
R3927	T6586	T6584	dobj	transgene,carrying
R3928	T6587	T6588	compound	YC5,EYFP
R3929	T6588	T6586	compound	EYFP,transgene
R393	T688	T674	aux	have,become
R3930	T6589	T6588	punct	/,EYFP
R3931	T6590	T6586	punct	(,transgene
R3932	T6591	T6586	amod	EYFP+,transgene
R3933	T6592	T6562	punct	),aggregated
R3934	T6593	T6562	punct	.,aggregated
R3935	T6595	T6596	det	An,vessel
R3936	T6596	T6598	nsubj	vessel,proliferated
R3937	T6597	T6596	amod	ECFP+,vessel
R3938	T6599	T6598	aux	has,proliferated
R3939	T6600	T6598	advmod	clonally,proliferated
R394	T689	T674	acomp	commonplace,become
R3940	T6601	T6598	cc	and,proliferated
R3941	T6602	T6598	conj	infiltrated,proliferated
R3942	T6603	T6604	det	the,lobe
R3943	T6604	T6602	dobj	lobe,infiltrated
R3944	T6605	T6604	compound	liver,lobe
R3945	T6606	T6607	punct	(,top
R3946	T6607	T6602	parataxis	top,infiltrated
R3947	T6608	T6607	punct	),top
R3948	T6609	T6598	punct	.,proliferated
R3949	T6612	T6613	mark	that,appears
R395	T690	T674	punct	.,become
R3950	T6613	T6611	ccomp	appears,Note
R3951	T6614	T6613	prep	unlike,appears
R3952	T6615	T6614	prep	in,unlike
R3953	T6616	T6615	pobj	heart,in
R3954	T6617	T6616	cc	and,heart
R3955	T6618	T6616	conj	pancreas,heart
R3956	T6619	T6613	expl	there,appears
R3957	T6620	T6621	aux	to,be
R3958	T6621	T6613	xcomp	be,appears
R3959	T6622	T6623	advmod	more,interspersed
R396	T692	T693	advmod	Genetically,manipulated
R3960	T6623	T6624	amod	interspersed,fluorescence
R3961	T6624	T6621	attr	fluorescence,be
R3962	T6625	T6626	amod	blue,cyan
R3963	T6626	T6624	amod	cyan,fluorescence
R3964	T6627	T6626	punct	/,cyan
R3965	T6628	T6626	cc	vs,cyan
R3966	T6629	T6630	amod	green,yellow
R3967	T6630	T6626	conj	yellow,cyan
R3968	T6631	T6630	punct	/,yellow
R3969	T6632	T6624	amod	present,fluorescence
R397	T693	T694	amod	manipulated,mice
R3970	T6633	T6632	prep	in,present
R3971	T6634	T6635	det	the,liver
R3972	T6635	T6633	pobj	liver,in
R3973	T6636	T6621	punct	", ",be
R3974	T6637	T6638	advmod	possibly,reflecting
R3975	T6638	T6621	advcl	reflecting,be
R3976	T6639	T6640	amod	greater,intermingling
R3977	T6640	T6638	dobj	intermingling,reflecting
R3978	T6641	T6640	compound	cell,intermingling
R3979	T6642	T6638	prep	during,reflecting
R398	T694	T695	nsubjpass	mice,used
R3980	T6643	T6644	det	the,genesis
R3981	T6644	T6642	pobj	genesis,during
R3982	T6645	T6644	prep	of,genesis
R3983	T6646	T6647	det	this,organ
R3984	T6647	T6645	pobj	organ,of
R3985	T6648	T6611	punct	.,Note
R399	T696	T694	punct	", ",mice
R40	T198	T195	pobj	years,In
R400	T697	T698	advmod	often,incorporating
R401	T698	T694	acl	incorporating,mice
R402	T699	T700	npadvmod	gene,based
R403	T700	T702	amod	based,reporters
R404	T701	T700	punct	-,based
R405	T702	T698	dobj	reporters,incorporating
R406	T703	T695	punct	", ",used
R407	T704	T695	aux	are,used
R408	T705	T695	advmod	frequently,used
R409	T706	T695	auxpass	being,used
R41	T199	T200	amod	green,protein
R410	T707	T708	aux	to,model
R411	T708	T695	advcl	model,used
R412	T709	T708	cc	and,model
R413	T710	T708	conj	understand,model
R414	T711	T712	amod	mammalian,development
R415	T712	T710	dobj	development,understand
R416	T713	T712	cc	and,development
R417	T714	T715	compound	disease,processes
R418	T715	T712	conj	processes,development
R419	T716	T695	punct	.,used
R42	T200	T196	nsubj	protein,gained
R420	T718	T719	amod	Fluorescent,protein
R421	T719	T720	compound	protein,reporters
R422	T720	T721	nsubj	reporters,represent
R423	T722	T721	advmod	currently,represent
R424	T723	T724	det	a,alternative
R425	T724	T721	dobj	alternative,represent
R426	T725	T724	amod	superior,alternative
R427	T726	T724	prep	to,alternative
R428	T727	T728	amod	other,reporters
R429	T728	T726	pobj	reporters,to
R43	T201	T200	amod	fluorescent,protein
R430	T729	T730	npadvmod	gene,based
R431	T730	T728	amod	based,reporters
R432	T731	T730	punct	-,based
R433	T732	T733	amod	such,as
R434	T733	T728	prep	as,reporters
R435	T734	T735	det	the,lacZ
R436	T735	T733	pobj	lacZ,as
R437	T736	T735	amod	bacterial,lacZ
R438	T737	T735	cc	or,lacZ
R439	T738	T739	amod	human,placental
R44	T202	T200	punct	(,protein
R440	T739	T740	amod	placental,phosphatase
R441	T740	T735	conj	phosphatase,lacZ
R442	T741	T740	amod	alkaline,phosphatase
R443	T742	T743	mark	in,is
R444	T743	T721	advcl	is,represent
R445	T744	T743	mark	that,is
R446	T745	T746	poss	their,visualization
R447	T746	T743	nsubj	visualization,is
R448	T747	T743	acomp	non-invasive,is
R449	T748	T743	punct	", ",is
R45	T203	T200	appos	GFP,protein
R450	T749	T743	cc	and,is
R451	T750	T751	prep	as,require
R452	T751	T743	conj	require,is
R453	T752	T750	amod	such,as
R454	T753	T751	aux	does,require
R455	T754	T751	neg	not,require
R456	T755	T756	amod	chromogenic,substrates
R457	T756	T751	dobj	substrates,require
R458	T757	T721	punct	.,represent
R459	T759	T760	nsubjpass	Fluorescence,monitored
R46	T204	T200	punct	),protein
R460	T761	T760	aux	can,monitored
R461	T762	T760	auxpass	be,monitored
R462	T763	T760	prep	in,monitored
R463	T764	T765	amod	real,time
R464	T765	T763	pobj	time,in
R465	T766	T765	punct	-,time
R466	T767	T768	advmod	in,vivo
R467	T768	T760	advmod	vivo,monitored
R468	T769	T768	cc	and,vivo
R469	T770	T771	advmod	in,situ
R47	T205	T200	prep	from,protein
R470	T771	T768	conj	situ,vivo
R471	T772	T760	punct	", ",monitored
R472	T773	T760	cc	and,monitored
R473	T774	T760	conj	has,monitored
R474	T775	T776	det	the,advantage
R475	T776	T774	dobj	advantage,has
R476	T777	T776	amod	added,advantage
R477	T778	T779	mark	in,quantified
R478	T779	T774	advcl	quantified,has
R479	T780	T779	mark	that,quantified
R48	T206	T207	det	the,jellyfish
R480	T781	T779	nsubjpass	it,quantified
R481	T782	T779	aux	can,quantified
R482	T783	T779	auxpass	be,quantified
R483	T784	T760	punct	.,monitored
R484	T786	T787	det	The,reporter
R485	T787	T791	nsubj	reporter,is
R486	T788	T787	nmod	prototype,reporter
R487	T789	T790	amod	fluorescent,protein
R488	T790	T787	compound	protein,reporter
R489	T792	T793	amod	green,protein
R49	T207	T205	pobj	jellyfish,from
R490	T793	T791	attr	protein,is
R491	T794	T793	amod	fluorescent,protein
R492	T795	T793	punct	(,protein
R493	T796	T793	appos	GFP,protein
R494	T797	T793	punct	", ",protein
R495	T798	T793	acl	reviewed,protein
R496	T799	T798	prep	in,reviewed
R497	T800	T799	punct	[,in
R498	T801	T799	pobj	3,in
R499	T802	T799	punct	],in
R50	T208	T207	amod	bioluminescent,jellyfish
R500	T803	T793	punct	),protein
R501	T804	T793	punct	", ",protein
R502	T805	T806	dep	which,derived
R503	T806	T793	relcl	derived,protein
R504	T807	T806	auxpass	is,derived
R505	T808	T806	prep	from,derived
R506	T809	T810	det	the,jellyfish
R507	T810	T808	pobj	jellyfish,from
R508	T811	T810	amod	bioluminescent,jellyfish
R509	T812	T813	compound	Aequorea,Victoria
R51	T209	T210	compound	Aequoria,Victoria
R510	T813	T810	appos	Victoria,jellyfish
R511	T814	T815	punct	[,1
R512	T815	T791	parataxis	1,is
R513	T816	T815	punct	],1
R514	T817	T791	punct	.,is
R515	T819	T820	amod	Green,variants
R516	T820	T822	nsubj	variants,gained
R517	T821	T820	amod	fluorescent,variants
R518	T823	T820	prep	of,variants
R519	T824	T825	amod	wild,type
R52	T210	T207	appos	Victoria,jellyfish
R520	T825	T826	compound	type,GFP
R521	T826	T823	pobj	GFP,of
R522	T827	T826	punct	(,GFP
R523	T828	T826	appos	wtGFP,GFP
R524	T829	T820	punct	),variants
R525	T830	T820	prep	with,variants
R526	T831	T832	amod	improved,thermostability
R527	T832	T830	pobj	thermostability,with
R528	T833	T832	cc	and,thermostability
R529	T834	T835	compound	fluorescence,emission
R53	T211	T196	aux	has,gained
R530	T835	T832	conj	emission,thermostability
R531	T836	T820	punct	", ",variants
R532	T837	T820	prep	including,variants
R533	T838	T839	amod	enhanced,protein
R534	T839	T837	pobj	protein,including
R535	T840	T839	amod	green,protein
R536	T841	T839	amod	fluorescent,protein
R537	T842	T839	punct	(,protein
R538	T843	T839	appos	EGFP,protein
R539	T844	T839	punct	", ",protein
R54	T212	T196	dobj	popularity,gained
R540	T845	T846	punct	[,4
R541	T846	T839	parataxis	4,protein
R542	T847	T846	punct	],4
R543	T848	T839	punct	),protein
R544	T849	T839	punct	", ",protein
R545	T850	T839	cc	and,protein
R546	T851	T839	conj	mMGFP,protein
R547	T852	T853	punct	[,5
R548	T853	T851	parataxis	5,mMGFP
R549	T854	T853	punct	],5
R55	T213	T196	prep	in,gained
R550	T855	T822	punct	", ",gained
R551	T856	T822	aux	have,gained
R552	T857	T822	dobj	popularity,gained
R553	T858	T822	prep	for,gained
R554	T859	T858	pobj	use,for
R555	T860	T859	prep	in,use
R556	T861	T860	pobj	mice,in
R557	T862	T822	punct	.,gained
R558	T864	T865	advmod	Recently,described
R559	T866	T865	advmod	however,described
R56	T214	T215	npadvmod	mouse,transgenic
R560	T867	T865	punct	", ",described
R561	T868	T869	amod	several,mutants
R562	T869	T865	nsubjpass	mutants,described
R563	T870	T869	amod	additional,mutants
R564	T871	T869	prep	of,mutants
R565	T872	T871	pobj	wtGFP,of
R566	T873	T869	punct	", ",mutants
R567	T874	T869	prep	with,mutants
R568	T875	T876	amod	altered,profiles
R569	T876	T874	pobj	profiles,with
R57	T215	T216	amod	transgenic,regimes
R570	T877	T876	nmod	excitation,profiles
R571	T878	T877	cc	and,excitation
R572	T879	T877	conj	emission,excitation
R573	T880	T876	amod	spectral,profiles
R574	T881	T882	punct	(,fluoresce
R575	T882	T876	parataxis	fluoresce,profiles
R576	T883	T882	advmod	i.e.,fluoresce
R577	T884	T882	prep	in,fluoresce
R578	T885	T884	pobj	colors,in
R579	T886	T887	amod	other,than
R58	T216	T213	pobj	regimes,in
R580	T887	T885	prep	than,colors
R581	T888	T887	pobj	green,than
R582	T889	T882	punct	),fluoresce
R583	T890	T876	punct	", ",profiles
R584	T891	T892	advmod	as,as
R585	T892	T876	cc	as,profiles
R586	T893	T892	advmod	well,as
R587	T894	T895	amod	improved,thermostability
R588	T895	T876	conj	thermostability,profiles
R589	T896	T895	cc	and,thermostability
R59	T217	T215	cc	and,transgenic
R590	T897	T895	conj	fluorescence,thermostability
R591	T898	T865	punct	", ",described
R592	T899	T865	aux	have,described
R593	T900	T865	auxpass	been,described
R594	T901	T902	punct	[,6
R595	T902	T865	parataxis	6,described
R596	T903	T902	nummod	4,6
R597	T904	T902	punct	",",6
R598	T905	T902	punct	],6
R599	T906	T865	punct	.,described
R60	T218	T219	npadvmod	gene,targeting
R600	T908	T909	amod	Dual,color
R601	T909	T910	compound	color,imaging
R602	T910	T911	nsubj	imaging,is
R603	T912	T910	prep	of,imaging
R604	T913	T914	amod	several,proteins
R605	T914	T912	pobj	proteins,of
R606	T915	T916	compound	GFP,variant
R607	T916	T914	compound	variant,proteins
R608	T917	T918	det	a,prospect
R609	T918	T911	attr	prospect,is
R61	T219	T215	conj	targeting,transgenic
R610	T919	T920	advmod	very,attractive
R611	T920	T918	amod	attractive,prospect
R612	T921	T911	cc	and,is
R613	T922	T923	aux	has,become
R614	T923	T911	conj	become,is
R615	T924	T925	det	a,procedure
R616	T925	T923	attr	procedure,become
R617	T926	T925	amod	standard,procedure
R618	T927	T925	prep	in,procedure
R619	T928	T929	compound	cell,biology
R62	T220	T221	punct	[,1
R620	T929	T927	pobj	biology,in
R621	T930	T931	punct	[,8
R622	T931	T923	parataxis	8,become
R623	T932	T931	nummod	7,8
R624	T933	T931	punct	",",8
R625	T934	T931	punct	],8
R626	T935	T911	punct	.,is
R627	T937	T938	nsubjpass	It,demonstrated
R628	T939	T938	aux	has,demonstrated
R629	T940	T938	advmod	also,demonstrated
R63	T221	T196	parataxis	1,gained
R630	T941	T938	advmod	recently,demonstrated
R631	T942	T938	auxpass	been,demonstrated
R632	T943	T938	prep	in,demonstrated
R633	T944	T945	det	a,subset
R634	T945	T943	pobj	subset,in
R635	T946	T945	prep	of,subset
R636	T947	T946	pobj	cells,of
R637	T948	T945	prep	within,subset
R638	T949	T950	det	the,system
R639	T950	T948	pobj	system,within
R64	T222	T221	punct	],1
R640	T951	T950	amod	central,system
R641	T952	T950	amod	nervous,system
R642	T953	T950	punct	(,system
R643	T954	T950	appos	CNS,system
R644	T955	T950	punct	),system
R645	T956	T950	prep	of,system
R646	T957	T956	pobj	mice,of
R647	T958	T938	advcl	using,demonstrated
R648	T959	T960	preconj	both,transgenic
R649	T960	T961	amod	transgenic,approaches
R65	T223	T196	punct	.,gained
R650	T961	T958	dobj	approaches,using
R651	T962	T960	cc	and,transgenic
R652	T963	T964	amod	viral,infection
R653	T964	T965	amod	infection,based
R654	T965	T960	conj	based,transgenic
R655	T966	T965	punct	-,based
R656	T967	T968	punct	[,10
R657	T968	T938	parataxis	10,demonstrated
R658	T969	T968	nummod	9,10
R659	T970	T968	punct	",",10
R66	T225	T226	nsubj	It,offers
R660	T971	T968	punct	],10
R661	T972	T938	punct	.,demonstrated
R662	T974	T975	nsubj	We,wished
R663	T976	T975	advmod	therefore,wished
R664	T977	T978	aux	to,investigate
R665	T978	T975	xcomp	investigate,wished
R666	T979	T980	det	the,feasibility
R667	T980	T978	dobj	feasibility,investigate
R668	T981	T980	prep	of,feasibility
R669	T982	T983	amod	non-invasive,imaging
R67	T227	T228	amod	several,advantages
R670	T983	T981	pobj	imaging,of
R671	T984	T985	amod	multiple,reporter
R672	T985	T983	compound	reporter,imaging
R673	T986	T983	prep	in,imaging
R674	T987	T986	pobj	mice,in
R675	T988	T975	punct	", ",wished
R676	T989	T975	cc	and,wished
R677	T990	T991	prep	as,wanted
R678	T991	T975	conj	wanted,wished
R679	T992	T993	det	a,step
R68	T228	T226	dobj	advantages,offers
R680	T993	T990	pobj	step,as
R681	T994	T993	amod	first,step
R682	T995	T991	nsubj	we,wanted
R683	T996	T997	aux	to,ascertain
R684	T997	T991	xcomp	ascertain,wanted
R685	T998	T999	mark	whether,establish
R686	T999	T997	ccomp	establish,ascertain
R687	T1000	T999	nsubj	we,establish
R688	T1001	T999	aux	could,establish
R689	T1002	T1003	amod	viable,mice
R69	T229	T228	prep	over,advantages
R690	T1003	T999	dobj	mice,establish
R691	T1004	T1002	cc	and,viable
R692	T1005	T1002	conj	fertile,viable
R693	T1006	T999	prep	with,establish
R694	T1007	T1008	amod	widespread,expression
R695	T1008	T1006	pobj	expression,with
R696	T1009	T1008	prep	of,expression
R697	T1010	T1011	compound	GFP,variant
R698	T1011	T1012	compound	variant,reporters
R699	T1012	T1009	pobj	reporters,of
R70	T230	T231	amod	conventional,reporters
R700	T1013	T991	punct	.,wanted
R705	T1399	T1400	nsubj	We,tested
R706	T1401	T1400	dobj	several,tested
R707	T1402	T1401	prep	of,several
R708	T1403	T1404	det	the,variants
R709	T1404	T1402	pobj	variants,of
R71	T231	T229	pobj	reporters,over
R710	T1405	T1404	amod	available,variants
R711	T1406	T1404	compound	wtGFP,variants
R712	T1407	T1400	prep	for,tested
R713	T1408	T1409	amod	developmental,neutrality
R714	T1409	T1407	pobj	neutrality,for
R715	T1410	T1409	cc	and,neutrality
R716	T1411	T1409	conj	stability,neutrality
R717	T1412	T1411	prep	of,stability
R718	T1413	T1414	amod	widespread,expression
R719	T1414	T1412	pobj	expression,of
R72	T232	T233	npadvmod	gene,based
R720	T1415	T1400	punct	.,tested
R721	T1417	T1418	det	An,approach
R722	T1418	T1424	nsubjpass	approach,used
R723	T1419	T1418	nmod	ES,approach
R724	T1420	T1421	npadvmod	cell,mediated
R725	T1421	T1418	amod	mediated,approach
R726	T1422	T1421	punct	-,mediated
R727	T1423	T1418	amod	transgenic,approach
R728	T1425	T1424	auxpass	was,used
R729	T1426	T1427	aux	to,evaluate
R73	T233	T231	amod	based,reporters
R730	T1427	T1424	advcl	evaluate,used
R731	T1428	T1429	det	the,Protein
R732	T1429	T1433	nmod	Protein,variants
R733	T1430	T1429	amod	Enhanced,Protein
R734	T1431	T1429	nmod	Cyan,Protein
R735	T1432	T1429	amod	Fluorescent,Protein
R736	T1433	T1427	dobj	variants,evaluate
R737	T1434	T1429	punct	(,Protein
R738	T1435	T1429	appos	ECFP,Protein
R739	T1436	T1429	punct	),Protein
R74	T234	T233	punct	-,based
R740	T1437	T1429	cc	and,Protein
R741	T1438	T1439	nmod	Enhanced,Protein
R742	T1439	T1429	conj	Protein,Protein
R743	T1440	T1439	amod	Yellow,Protein
R744	T1441	T1439	amod	Fluorescent,Protein
R745	T1442	T1439	punct	(,Protein
R746	T1443	T1439	appos	EYFP,Protein
R747	T1444	T1433	punct	),variants
R748	T1445	T1433	nmod	wtGFP,variants
R749	T1446	T1433	amod	spectral,variants
R75	T235	T228	punct	", ",advantages
R750	T1447	T1448	punct	[,11
R751	T1448	T1424	parataxis	11,used
R752	T1449	T1448	nummod	4,11
R753	T1450	T1448	punct	",",11
R754	T1451	T1448	punct	],11
R755	T1452	T1424	punct	.,used
R756	T1454	T1455	nsubj	ECFP,harbors
R757	T1456	T1457	nummod	six,substitutions
R758	T1457	T1455	dobj	substitutions,harbors
R759	T1458	T1457	compound	amino,substitutions
R76	T236	T237	amod	such,as
R760	T1459	T1457	punct	(,substitutions
R761	T1460	T1461	nmod	Y66W,V163A
R762	T1461	T1457	appos	V163A,substitutions
R763	T1462	T1461	punct	", ",V163A
R764	T1463	T1461	nmod	F64L,V163A
R765	T1464	T1461	punct	", ",V163A
R766	T1465	T1461	nmod	S65T,V163A
R767	T1466	T1461	punct	", ",V163A
R768	T1467	T1461	nmod	N146I,V163A
R769	T1468	T1461	punct	", ",V163A
R77	T237	T228	prep	as,advantages
R770	T1469	T1461	nmod	M153T,V163A
R771	T1470	T1461	punct	", ",V163A
R772	T1471	T1455	punct	),harbors
R773	T1472	T1455	prep	as,harbors
R774	T1473	T1472	pcomp	compared,as
R775	T1474	T1472	prep	to,as
R776	T1475	T1474	pobj	GFP,to
R777	T1476	T1455	punct	", ",harbors
R778	T1477	T1478	mark	whereas,harbors
R779	T1478	T1455	advcl	harbors,harbors
R78	T238	T237	pobj	lacZ,as
R780	T1479	T1478	nsubj	EYFP,harbors
R781	T1480	T1478	dobj	four,harbors
R782	T1481	T1480	punct	(,four
R783	T1482	T1480	appos	S65G,four
R784	T1483	T1482	punct	", ",S65G
R785	T1484	T1482	conj	V68L,S65G
R786	T1485	T1484	punct	", ",V68L
R787	T1486	T1484	conj	S72A,V68L
R788	T1487	T1486	cc	and,S72A
R789	T1488	T1486	conj	T203Y,S72A
R79	T239	T238	cc	and,lacZ
R790	T1489	T1455	punct	),harbors
R791	T1490	T1455	punct	.,harbors
R792	T1492	T1493	prep	In,shift
R793	T1494	T1492	pobj	addition,In
R794	T1495	T1494	prep	to,addition
R795	T1496	T1495	pcomp	increasing,to
R796	T1497	T1498	det	the,yield
R797	T1498	T1496	dobj	yield,increasing
R798	T1499	T1498	compound	quantum,yield
R799	T1500	T1498	cc	and,yield
R80	T240	T241	compound	alkaline,phosphatase
R800	T1501	T1498	conj	solubility,yield
R801	T1502	T1498	prep	of,yield
R802	T1503	T1504	det	the,proteins
R803	T1504	T1502	pobj	proteins,of
R804	T1505	T1506	det	these,mutations
R805	T1506	T1493	nsubj	mutations,shift
R806	T1507	T1508	det	the,profiles
R807	T1508	T1493	dobj	profiles,shift
R808	T1509	T1508	amod	spectral,profiles
R809	T1510	T1508	prep	of,profiles
R81	T241	T238	conj	phosphatase,lacZ
R810	T1511	T1512	det	the,products
R811	T1512	T1510	pobj	products,of
R812	T1513	T1512	compound	translation,products
R813	T1514	T1515	punct	[,11
R814	T1515	T1493	parataxis	11,shift
R815	T1516	T1515	nummod	4,11
R816	T1517	T1515	punct	",",11
R817	T1518	T1515	punct	],11
R818	T1519	T1493	punct	.,shift
R819	T1521	T1522	det	A,breakthrough
R82	T242	T226	punct	", ",offers
R820	T1522	T1523	nsubj	breakthrough,came
R821	T1524	T1522	prep	in,breakthrough
R822	T1525	T1526	det	the,availability
R823	T1526	T1524	pobj	availability,in
R824	T1527	T1526	prep	of,availability
R825	T1528	T1529	advmod	spectrally,distinct
R826	T1529	T1530	amod	distinct,proteins
R827	T1530	T1527	pobj	proteins,of
R828	T1531	T1530	amod	autofluorescent,proteins
R829	T1532	T1523	prep	with,came
R83	T243	T244	mark	in,require
R830	T1533	T1534	det	the,cloning
R831	T1534	T1532	pobj	cloning,with
R832	T1535	T1534	prep	of,cloning
R833	T1536	T1537	nummod	six,proteins
R834	T1537	T1535	pobj	proteins,of
R835	T1538	T1537	nmod	anthozoan,proteins
R836	T1539	T1537	amod	fluorescent,proteins
R837	T1540	T1541	advmod	all,having
R838	T1541	T1537	relcl	having,proteins
R839	T1542	T1543	quantmod	20,30
R84	T244	T226	advcl	require,offers
R840	T1543	T1545	nummod	30,%
R841	T1544	T1543	punct	–,30
R842	T1545	T1546	compound	%,identity
R843	T1546	T1541	dobj	identity,having
R844	T1547	T1546	prep	to,identity
R845	T1548	T1547	pobj	wtGFP,to
R846	T1549	T1550	punct	[,12
R847	T1550	T1523	parataxis	12,came
R848	T1551	T1550	punct	],12
R849	T1552	T1523	punct	.,came
R85	T245	T244	mark	that,require
R850	T1554	T1555	prep	Of,posseses
R851	T1556	T1554	pobj	these,Of
R852	T1557	T1555	punct	", ",posseses
R853	T1558	T1555	nsubj	DsRed1,posseses
R854	T1559	T1558	punct	(,DsRed1
R855	T1560	T1558	acl	referred,DsRed1
R856	T1561	T1560	prep	to,referred
R857	T1562	T1560	prep	as,referred
R858	T1563	T1562	pobj	drFP585,as
R859	T1564	T1560	agent	by,referred
R86	T246	T247	poss	its,visualization
R860	T1565	T1564	pobj	Matz,by
R861	T1566	T1567	advmod	et,al.
R862	T1567	T1565	advmod	al.,Matz
R863	T1568	T1560	punct	", ",referred
R864	T1569	T1570	punct	[,12
R865	T1570	T1560	parataxis	12,referred
R866	T1571	T1570	punct	],12
R867	T1572	T1558	punct	),DsRed1
R868	T1573	T1558	punct	", ",DsRed1
R869	T1574	T1575	det	a,protein
R87	T247	T244	nsubj	visualization,require
R870	T1575	T1558	appos	protein,DsRed1
R871	T1576	T1577	nummod	28,kDa
R872	T1577	T1575	compound	kDa,protein
R873	T1578	T1575	acl	isolated,protein
R874	T1579	T1578	prep	from,isolated
R875	T1580	T1581	det	the,anemone
R876	T1581	T1579	pobj	anemone,from
R877	T1582	T1581	compound	IndoPacific,anemone
R878	T1583	T1581	compound	sea,anemone
R879	T1584	T1585	compound	Discosoma,sp
R88	T248	T244	aux	does,require
R880	T1585	T1581	appos	sp,anemone
R881	T1586	T1555	punct	", ",posseses
R882	T1587	T1588	det	the,maxima
R883	T1588	T1555	dobj	maxima,posseses
R884	T1589	T1588	amod	longest,maxima
R885	T1590	T1588	nmod	excitation,maxima
R886	T1591	T1592	punct	(,nm
R887	T1592	T1590	parataxis	nm,excitation
R888	T1593	T1592	nummod	558,nm
R889	T1594	T1592	punct	),nm
R89	T249	T244	neg	not,require
R890	T1595	T1590	cc	and,excitation
R891	T1596	T1590	conj	emission,excitation
R892	T1597	T1598	punct	(,nm
R893	T1598	T1596	parataxis	nm,emission
R894	T1599	T1598	nummod	583,nm
R895	T1600	T1598	punct	),nm
R896	T1601	T1588	compound	wavelength,maxima
R897	T1602	T1588	acl	known,maxima
R898	T1603	T1602	prep	for,known
R899	T1604	T1605	det	a,protein
R9	T160	T158	compound	stem,cells
R90	T250	T251	det	a,substrate
R900	T1605	T1603	pobj	protein,for
R901	T1606	T1607	amod	wild,type
R902	T1607	T1605	nmod	type,protein
R903	T1608	T1607	punct	-,type
R904	T1609	T1605	amod	autofluorescent,protein
R905	T1610	T1555	punct	.,posseses
R906	T1612	T1613	det	The,sequence
R907	T1613	T1614	nsubjpass	sequence,modified
R908	T1615	T1613	prep	of,sequence
R909	T1616	T1615	pobj	DsRed1,of
R91	T251	T244	dobj	substrate,require
R910	T1617	T1614	aux	has,modified
R911	T1618	T1614	advmod	recently,modified
R912	T1619	T1614	auxpass	been,modified
R913	T1620	T1614	cc	and,modified
R914	T1621	T1614	conj	optimized,modified
R915	T1622	T1621	prep	for,optimized
R916	T1623	T1624	amod	high,levels
R917	T1624	T1622	pobj	levels,for
R918	T1625	T1624	prep	of,levels
R919	T1626	T1625	pobj	expression,of
R92	T252	T251	amod	chromogenic,substrate
R920	T1627	T1624	prep	in,levels
R921	T1628	T1629	amod	mammalian,cells
R922	T1629	T1627	pobj	cells,in
R923	T1630	T1621	prep	by,optimized
R924	T1631	T1632	det	the,introduction
R925	T1632	T1630	pobj	introduction,by
R926	T1633	T1632	prep	of,introduction
R927	T1634	T1635	nummod	144,changes
R928	T1635	T1633	pobj	changes,of
R929	T1636	T1637	amod	silent,pair
R93	T253	T244	cc	and,require
R930	T1637	T1635	compound	pair,changes
R931	T1638	T1637	compound	base,pair
R932	T1639	T1635	acl	corresponding,changes
R933	T1640	T1639	prep	to,corresponding
R934	T1641	T1642	amod	human,codon
R935	T1642	T1643	compound	codon,usage
R936	T1643	T1644	compound	usage,preferences
R937	T1644	T1640	pobj	preferences,to
R938	T1645	T1614	punct	.,modified
R939	T1647	T1648	nsubj	We,tested
R94	T254	T255	aux	can,realized
R940	T1649	T1648	advmod	therefore,tested
R941	T1650	T1648	advmod	also,tested
R942	T1651	T1652	det	this,protein
R943	T1652	T1648	dobj	protein,tested
R944	T1653	T1652	amod	novel,protein
R945	T1654	T1652	amod	red,protein
R946	T1655	T1652	amod	fluorescent,protein
R947	T1656	T1652	punct	(,protein
R948	T1657	T1652	appos	RFP,protein
R949	T1658	T1648	punct	),tested
R95	T255	T244	conj	realized,require
R950	T1659	T1648	prep	in,tested
R951	T1660	T1661	compound	ES,cells
R952	T1661	T1659	pobj	cells,in
R953	T1662	T1661	cc	and,cells
R954	T1663	T1661	conj	mice,cells
R955	T1664	T1648	punct	.,tested
R956	T1666	T1667	nsubjpass	Plasmids,constructed
R957	T1668	T1667	auxpass	were,constructed
R958	T1669	T1667	prep	for,constructed
R959	T1670	T1669	pcomp	driving,for
R96	T256	T255	auxpass	be,realized
R960	T1671	T1672	amod	widespread,expression
R961	T1672	T1670	dobj	expression,driving
R962	T1673	T1672	prep	of,expression
R963	T1674	T1673	pobj	each,of
R964	T1675	T1674	prep	of,each
R965	T1676	T1677	det	the,reporters
R966	T1677	T1675	pobj	reporters,of
R967	T1678	T1679	npadvmod	gene,based
R968	T1679	T1677	amod	based,reporters
R969	T1680	T1679	punct	-,based
R97	T257	T258	advmod	in,vivo
R970	T1681	T1682	amod	fluorescent,protein
R971	T1682	T1677	nmod	protein,reporters
R972	T1683	T1684	punct	(,FP
R973	T1684	T1682	appos	FP,protein
R974	T1685	T1677	punct	),reporters
R975	T1686	T1667	cc	and,constructed
R976	T1687	T1688	auxpass	were,tested
R977	T1688	T1667	conj	tested,constructed
R978	T1689	T1688	prep	in,tested
R979	T1690	T1691	compound	ES,cell
R98	T258	T255	advmod	vivo,realized
R980	T1691	T1692	npadvmod	cell,mediated
R981	T1692	T1694	amod	mediated,transgenics
R982	T1693	T1692	punct	-,mediated
R983	T1694	T1689	pobj	transgenics,in
R984	T1695	T1696	mark	as,described
R985	T1696	T1688	advcl	described,tested
R986	T1697	T1696	advmod	previously,described
R987	T1698	T1699	punct	[,2
R988	T1699	T1688	parataxis	2,tested
R989	T1700	T1699	punct	],2
R99	T259	T226	punct	.,offers
R990	T1701	T1667	punct	.,constructed
R991	T1703	T1704	amod	Transgenic,cells
R992	T1704	T1706	nsubjpass	cells,obtained
R993	T1705	T1704	compound	ES,cells
R994	T1707	T1704	acl	exhibiting,cells
R995	T1708	T1709	amod	robust,expression
R996	T1709	T1707	dobj	expression,exhibiting
R997	T1710	T1709	prep	of,expression
R998	T1711	T1710	pobj	ECFP,of
R999	T1712	T1711	cc	or,ECFP

PMC:116589 JSONTXT 8 Projects

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PMC:116589 JSON TXT 8 Projects