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PMC:1142324 / 3794-11657 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1243 9-19 NN denotes Generation
T1244 20-22 IN denotes of
T1245 23-29 NN denotes ADAM22
T1247 29-30 HYPH denotes -
T1246 30-39 JJ denotes deficient
T1248 40-44 NNS denotes mice
T1249 44-153 sentence denotes Mice carrying a targeted mutation in their Adam22 gene were generated by homologous recombination (Fig. 1A).
T1250 45-49 NNS denotes Mice
T1252 50-58 VBG denotes carrying
T1253 59-60 DT denotes a
T1255 61-69 VBN denotes targeted
T1254 70-78 NN denotes mutation
T1256 79-81 IN denotes in
T1257 82-87 PRP$ denotes their
T1259 88-94 NN denotes Adam22
T1258 95-99 NN denotes gene
T1260 100-104 VBD denotes were
T1251 105-114 VBN denotes generated
T1261 115-117 IN denotes by
T1262 118-128 JJ denotes homologous
T1263 129-142 NN denotes recombination
T1264 143-144 -LRB- denotes (
T1266 144-148 NN denotes Fig.
T1265 149-151 NN denotes 1A
T1267 151-152 -RRB- denotes )
T1268 152-153 . denotes .
T1269 153-230 sentence denotes Correct targeting events were confirmed by Southern blot analysis (Fig. 1B).
T1270 154-161 JJ denotes Correct
T1272 162-171 VBG denotes targeting
T1271 172-178 NNS denotes events
T1274 179-183 VBD denotes were
T1273 184-193 VBN denotes confirmed
T1275 194-196 IN denotes by
T1276 197-205 NNP denotes Southern
T1277 206-210 NN denotes blot
T1278 211-219 NN denotes analysis
T1279 220-221 -LRB- denotes (
T1281 221-225 NN denotes Fig.
T1280 226-228 NN denotes 1B
T1282 228-229 -RRB- denotes )
T1283 229-230 . denotes .
T1284 230-404 sentence denotes Since the termination codon was introduced in exon 8 in the pro-protein domain, only the truncated form of the ADAM22 protein would be synthesized from this targeted allele.
T1285 231-236 IN denotes Since
T1287 237-240 DT denotes the
T1289 241-252 NN denotes termination
T1288 253-258 NN denotes codon
T1290 259-262 VBD denotes was
T1286 263-273 VBN denotes introduced
T1292 274-276 IN denotes in
T1293 277-281 NN denotes exon
T1294 282-283 CD denotes 8
T1295 284-286 IN denotes in
T1296 287-290 DT denotes the
T1298 291-302 JJ denotes pro-protein
T1297 303-309 NN denotes domain
T1299 309-311 , denotes ,
T1300 311-315 RB denotes only
T1302 316-319 DT denotes the
T1303 320-329 VBN denotes truncated
T1301 330-334 NN denotes form
T1304 335-337 IN denotes of
T1305 338-341 DT denotes the
T1307 342-348 NN denotes ADAM22
T1306 349-356 NN denotes protein
T1308 357-362 MD denotes would
T1309 363-365 VB denotes be
T1291 366-377 VBN denotes synthesized
T1310 378-382 IN denotes from
T1311 383-387 DT denotes this
T1313 388-396 VBN denotes targeted
T1312 397-403 NN denotes allele
T1314 403-404 . denotes .
T1315 404-636 sentence denotes Because a pro-protein domain is always removed in the mature functional ADAM-proteins by the Furin-like proteases and is thought to be non-functional itself, we considered that this truncated form of ADAM22 protein has no function.
T1316 405-412 IN denotes Because
T1318 413-414 DT denotes a
T1320 415-426 JJ denotes pro-protein
T1319 427-433 NN denotes domain
T1321 434-436 VBZ denotes is
T1322 437-443 RB denotes always
T1317 444-451 VBN denotes removed
T1324 452-454 IN denotes in
T1325 455-458 DT denotes the
T1327 459-465 JJ denotes mature
T1328 466-476 JJ denotes functional
T1329 477-481 NN denotes ADAM
T1330 481-482 HYPH denotes -
T1326 482-490 NN denotes proteins
T1331 491-493 IN denotes by
T1332 494-497 DT denotes the
T1334 498-503 NN denotes Furin
T1336 503-504 HYPH denotes -
T1335 504-508 JJ denotes like
T1333 509-518 NNS denotes proteases
T1337 519-522 CC denotes and
T1338 523-525 VBZ denotes is
T1339 526-533 VBN denotes thought
T1340 534-536 TO denotes to
T1341 537-539 VB denotes be
T1342 540-554 JJ denotes non-functional
T1343 555-561 PRP denotes itself
T1344 561-563 , denotes ,
T1345 563-565 PRP denotes we
T1323 566-576 VBD denotes considered
T1346 577-581 IN denotes that
T1348 582-586 DT denotes this
T1350 587-596 VBN denotes truncated
T1349 597-601 NN denotes form
T1351 602-604 IN denotes of
T1352 605-611 NN denotes ADAM22
T1353 612-619 NN denotes protein
T1347 620-623 VBZ denotes has
T1354 624-626 DT denotes no
T1355 627-635 NN denotes function
T1356 635-636 . denotes .
T1357 636-832 sentence denotes Absence of mature ADAM22 protein in homozygous mutants was confirmed by Western blot analysis using the specific antibody, which recognizes the cytoplasmic domain of the ADAM22 protein (Fig. 1C).
T1358 637-644 NN denotes Absence
T1360 645-647 IN denotes of
T1361 648-654 JJ denotes mature
T1363 655-661 NN denotes ADAM22
T1362 662-669 NN denotes protein
T1364 670-672 IN denotes in
T1365 673-683 JJ denotes homozygous
T1366 684-691 NNS denotes mutants
T1367 692-695 VBD denotes was
T1359 696-705 VBN denotes confirmed
T1368 706-708 IN denotes by
T1369 709-716 NNP denotes Western
T1370 717-721 NN denotes blot
T1371 722-730 NN denotes analysis
T1372 731-736 VBG denotes using
T1373 737-740 DT denotes the
T1375 741-749 JJ denotes specific
T1374 750-758 NN denotes antibody
T1376 758-760 , denotes ,
T1377 760-765 WDT denotes which
T1378 766-776 VBZ denotes recognizes
T1379 777-780 DT denotes the
T1381 781-792 JJ denotes cytoplasmic
T1380 793-799 NN denotes domain
T1382 800-802 IN denotes of
T1383 803-806 DT denotes the
T1385 807-813 NN denotes ADAM22
T1384 814-821 NN denotes protein
T1386 822-823 -LRB- denotes (
T1388 823-827 NN denotes Fig.
T1387 828-830 NN denotes 1C
T1389 830-831 -RRB- denotes )
T1390 831-832 . denotes .
T1391 832-982 sentence denotes Homozygous mutants showed no noticeable defects at birth and were indistinguishable from wild-type or heterozygous littermates during the first week.
T1392 833-843 JJ denotes Homozygous
T1393 844-851 NNS denotes mutants
T1394 852-858 VBD denotes showed
T1395 859-861 DT denotes no
T1397 862-872 JJ denotes noticeable
T1396 873-880 NNS denotes defects
T1398 881-883 IN denotes at
T1399 884-889 NN denotes birth
T1400 890-893 CC denotes and
T1401 894-898 VBD denotes were
T1402 899-916 JJ denotes indistinguishable
T1403 917-921 IN denotes from
T1404 922-926 JJ denotes wild
T1406 926-927 HYPH denotes -
T1405 927-931 NN denotes type
T1408 932-934 CC denotes or
T1409 935-947 JJ denotes heterozygous
T1407 948-959 NNS denotes littermates
T1410 960-966 IN denotes during
T1411 967-970 DT denotes the
T1413 971-976 JJ denotes first
T1412 977-981 NN denotes week
T1414 981-982 . denotes .
T1415 982-1150 sentence denotes At postnatal day 10 (P10), most of the homozygous mutants were distinguishable by abnormalities such as reduced body weight and uncoordinated movements of their limbs.
T1416 983-985 IN denotes At
T1418 986-995 JJ denotes postnatal
T1419 996-999 NN denotes day
T1420 1000-1002 CD denotes 10
T1421 1003-1004 -LRB- denotes (
T1422 1004-1007 NN denotes P10
T1423 1007-1008 -RRB- denotes )
T1424 1008-1010 , denotes ,
T1425 1010-1014 JJS denotes most
T1426 1015-1017 IN denotes of
T1427 1018-1021 DT denotes the
T1429 1022-1032 JJ denotes homozygous
T1428 1033-1040 NNS denotes mutants
T1417 1041-1045 VBD denotes were
T1430 1046-1061 JJ denotes distinguishable
T1431 1062-1064 IN denotes by
T1432 1065-1078 NNS denotes abnormalities
T1433 1079-1083 JJ denotes such
T1434 1084-1086 IN denotes as
T1435 1087-1094 VBN denotes reduced
T1437 1095-1099 NN denotes body
T1436 1100-1106 NN denotes weight
T1438 1107-1110 CC denotes and
T1439 1111-1124 JJ denotes uncoordinated
T1440 1125-1134 NNS denotes movements
T1441 1135-1137 IN denotes of
T1442 1138-1143 PRP$ denotes their
T1443 1144-1149 NNS denotes limbs
T1444 1149-1150 . denotes .
T1445 1150-1228 sentence denotes After P10, all homozygotes displayed severe ataxia (Fig. 2) and began to die.
T1446 1151-1156 IN denotes After
T1448 1157-1160 NN denotes P10
T1449 1160-1162 , denotes ,
T1450 1162-1165 DT denotes all
T1451 1166-1177 NNS denotes homozygotes
T1447 1178-1187 VBD denotes displayed
T1452 1188-1194 JJ denotes severe
T1453 1195-1201 NN denotes ataxia
T1454 1202-1203 -LRB- denotes (
T1455 1203-1207 NN denotes Fig.
T1456 1208-1209 CD denotes 2
T1457 1209-1210 -RRB- denotes )
T1458 1211-1214 CC denotes and
T1459 1215-1220 VBD denotes began
T1460 1221-1223 TO denotes to
T1461 1224-1227 VB denotes die
T1462 1227-1228 . denotes .
T1463 1228-1380 sentence denotes To measure the survival rate and body weight of each genotype precisely, we backcrossed heterozygous male mutants to C57BL/6 females more than 6 times.
T1464 1229-1231 TO denotes To
T1465 1232-1239 VB denotes measure
T1467 1240-1243 DT denotes the
T1469 1244-1252 NN denotes survival
T1468 1253-1257 NN denotes rate
T1470 1258-1261 CC denotes and
T1471 1262-1266 NN denotes body
T1472 1267-1273 NN denotes weight
T1473 1274-1276 IN denotes of
T1474 1277-1281 DT denotes each
T1475 1282-1290 NN denotes genotype
T1476 1291-1300 RB denotes precisely
T1477 1300-1302 , denotes ,
T1478 1302-1304 PRP denotes we
T1466 1305-1316 VBD denotes backcrossed
T1479 1317-1329 JJ denotes heterozygous
T1481 1330-1334 JJ denotes male
T1480 1335-1342 NNS denotes mutants
T1482 1343-1345 IN denotes to
T1483 1346-1351 NN denotes C57BL
T1485 1351-1352 HYPH denotes /
T1486 1352-1353 CD denotes 6
T1484 1354-1361 NNS denotes females
T1487 1362-1366 JJR denotes more
T1489 1367-1371 IN denotes than
T1488 1372-1373 CD denotes 6
T1490 1374-1379 NNS denotes times
T1491 1379-1380 . denotes .
T1492 1380-1491 sentence denotes The resulting (N 6) heterozygous males and females were intercrossed and the produced offspring were analysed.
T1493 1381-1384 DT denotes The
T1495 1385-1394 VBG denotes resulting
T1496 1395-1396 -LRB- denotes (
T1497 1396-1397 NN denotes N
T1498 1398-1399 CD denotes 6
T1499 1399-1400 -RRB- denotes )
T1500 1401-1413 JJ denotes heterozygous
T1494 1414-1419 NNS denotes males
T1502 1420-1423 CC denotes and
T1503 1424-1431 NNS denotes females
T1504 1432-1436 VBD denotes were
T1501 1437-1449 VBN denotes intercrossed
T1505 1450-1453 CC denotes and
T1506 1454-1457 DT denotes the
T1508 1458-1466 VBN denotes produced
T1507 1467-1476 NN denotes offspring
T1510 1477-1481 VBD denotes were
T1509 1482-1490 VBN denotes analysed
T1511 1490-1491 . denotes .
T1512 1491-1568 sentence denotes The numbers of survivors of each genotype every 5 days are shown in Table 1.
T1513 1492-1495 DT denotes The
T1514 1496-1503 NNS denotes numbers
T1516 1504-1506 IN denotes of
T1517 1507-1516 NNS denotes survivors
T1518 1517-1519 IN denotes of
T1519 1520-1524 DT denotes each
T1520 1525-1533 NN denotes genotype
T1521 1534-1539 DT denotes every
T1523 1540-1541 CD denotes 5
T1522 1542-1546 NNS denotes days
T1524 1547-1550 VBP denotes are
T1515 1551-1556 VBN denotes shown
T1525 1557-1559 IN denotes in
T1526 1560-1565 NN denotes Table
T1527 1566-1567 CD denotes 1
T1528 1567-1568 . denotes .
T1529 1568-1696 sentence denotes At P10, the ratio of each genotype was in close accordance with the Mendelian ratio (20.5% +/+, 55.1% +/-, 24.4% -/- ; n = 78).
T1530 1569-1571 IN denotes At
T1532 1572-1575 NN denotes P10
T1533 1575-1577 , denotes ,
T1534 1577-1580 DT denotes the
T1535 1581-1586 NN denotes ratio
T1536 1587-1589 IN denotes of
T1537 1590-1594 DT denotes each
T1538 1595-1603 NN denotes genotype
T1531 1604-1607 VBD denotes was
T1539 1608-1610 IN denotes in
T1540 1611-1616 JJ denotes close
T1541 1617-1627 NN denotes accordance
T1542 1628-1632 IN denotes with
T1543 1633-1636 DT denotes the
T1545 1637-1646 JJ denotes Mendelian
T1544 1647-1652 NN denotes ratio
T1546 1653-1654 -LRB- denotes (
T1548 1654-1658 CD denotes 20.5
T1547 1658-1659 NN denotes %
T1549 1660-1661 SYM denotes +
T1550 1661-1662 HYPH denotes /
T1551 1662-1663 SYM denotes +
T1552 1663-1665 , denotes ,
T1553 1665-1669 CD denotes 55.1
T1554 1669-1670 NN denotes %
T1555 1671-1672 SYM denotes +
T1556 1672-1673 HYPH denotes /
T1557 1673-1674 SYM denotes -
T1558 1674-1676 , denotes ,
T1559 1676-1680 CD denotes 24.4
T1560 1680-1681 NN denotes %
T1561 1682-1683 SYM denotes -
T1562 1683-1684 HYPH denotes /
T1563 1684-1685 SYM denotes -
T1564 1686-1687 : denotes ;
T1565 1688-1689 NN denotes n
T1567 1690-1691 SYM denotes =
T1566 1692-1694 CD denotes 78
T1568 1694-1695 -RRB- denotes )
T1569 1695-1696 . denotes .
T1570 1696-1762 sentence denotes This result shows that ADAM22 is not essential for embryogenesis.
T1571 1697-1701 DT denotes This
T1572 1702-1708 NN denotes result
T1573 1709-1714 VBZ denotes shows
T1574 1715-1719 IN denotes that
T1576 1720-1726 NN denotes ADAM22
T1575 1727-1729 VBZ denotes is
T1577 1730-1733 RB denotes not
T1578 1734-1743 JJ denotes essential
T1579 1744-1747 IN denotes for
T1580 1748-1761 NN denotes embryogenesis
T1581 1761-1762 . denotes .
T1582 1762-1897 sentence denotes At P10, the average body weight of homozygous mutants was approximately half that of wild-type and heterozygous littermates (Table 2).
T1583 1763-1765 IN denotes At
T1585 1766-1769 NN denotes P10
T1586 1769-1771 , denotes ,
T1587 1771-1774 DT denotes the
T1589 1775-1782 JJ denotes average
T1590 1783-1787 NN denotes body
T1588 1788-1794 NN denotes weight
T1591 1795-1797 IN denotes of
T1592 1798-1808 JJ denotes homozygous
T1593 1809-1816 NNS denotes mutants
T1584 1817-1820 VBD denotes was
T1594 1821-1834 RB denotes approximately
T1595 1835-1839 NN denotes half
T1596 1840-1844 DT denotes that
T1597 1845-1847 IN denotes of
T1598 1848-1852 JJ denotes wild
T1600 1852-1853 HYPH denotes -
T1599 1853-1857 NN denotes type
T1602 1858-1861 CC denotes and
T1603 1862-1874 JJ denotes heterozygous
T1601 1875-1886 NNS denotes littermates
T1604 1887-1888 -LRB- denotes (
T1605 1888-1893 NN denotes Table
T1606 1894-1895 CD denotes 2
T1607 1895-1896 -RRB- denotes )
T1608 1896-1897 . denotes .
T1609 1897-1980 sentence denotes Homozygous mutants died one by one after P10, and all homozygotes died before P20.
T1610 1898-1908 JJ denotes Homozygous
T1611 1909-1916 NNS denotes mutants
T1612 1917-1921 VBD denotes died
T1613 1922-1925 CD denotes one
T1614 1926-1928 IN denotes by
T1615 1929-1932 CD denotes one
T1616 1933-1938 IN denotes after
T1617 1939-1942 NN denotes P10
T1618 1942-1944 , denotes ,
T1619 1944-1947 CC denotes and
T1620 1948-1951 DT denotes all
T1621 1952-1963 NNS denotes homozygotes
T1622 1964-1968 VBD denotes died
T1623 1969-1975 IN denotes before
T1624 1976-1979 NN denotes P20
T1625 1979-1980 . denotes .
T1626 1980-2077 sentence denotes Of more than 100 homozygotes we have produced, none have survived more than 25 days after birth.
T1627 1981-1983 IN denotes Of
T1629 1984-1988 JJR denotes more
T1631 1989-1993 IN denotes than
T1630 1994-1997 CD denotes 100
T1632 1998-2009 NNS denotes homozygotes
T1633 2010-2012 PRP denotes we
T1635 2013-2017 VBP denotes have
T1634 2018-2026 VBN denotes produced
T1636 2026-2028 , denotes ,
T1637 2028-2032 NN denotes none
T1638 2033-2037 VBP denotes have
T1628 2038-2046 VBN denotes survived
T1639 2047-2051 JJR denotes more
T1641 2052-2056 IN denotes than
T1640 2057-2059 CD denotes 25
T1642 2060-2064 NNS denotes days
T1643 2065-2070 IN denotes after
T1644 2071-2076 NN denotes birth
T1645 2076-2077 . denotes .
T1646 2077-2199 sentence denotes Meanwhile, heterozygous mutants looked normal, were fertile, and survived for more than one year without obvious defects.
T1647 2078-2087 RB denotes Meanwhile
T1649 2087-2089 , denotes ,
T1650 2089-2101 JJ denotes heterozygous
T1651 2102-2109 NNS denotes mutants
T1648 2110-2116 VBD denotes looked
T1652 2117-2123 JJ denotes normal
T1653 2123-2125 , denotes ,
T1654 2125-2129 VBD denotes were
T1655 2130-2137 JJ denotes fertile
T1656 2137-2139 , denotes ,
T1657 2139-2142 CC denotes and
T1658 2143-2151 VBD denotes survived
T1659 2152-2155 IN denotes for
T1660 2156-2160 JJR denotes more
T1662 2161-2165 IN denotes than
T1661 2166-2169 CD denotes one
T1663 2170-2174 NN denotes year
T1664 2175-2182 IN denotes without
T1665 2183-2190 JJ denotes obvious
T1666 2191-2198 NNS denotes defects
T1667 2198-2199 . denotes .
T1960 2201-2215 NN denotes Histopathology
T1961 2216-2218 IN denotes of
T1962 2219-2225 NN denotes ADAM22
T1964 2225-2226 HYPH denotes -
T1963 2226-2235 JJ denotes deficient
T1965 2236-2240 NNS denotes mice
T1966 2240-2363 sentence denotes We have reported the predominant expression of ADAM22 mRNA in the human and mouse brain by Northern blot analysis [13,14].
T1967 2241-2243 PRP denotes We
T1969 2244-2248 VBP denotes have
T1968 2249-2257 VBN denotes reported
T1970 2258-2261 DT denotes the
T1972 2262-2273 JJ denotes predominant
T1971 2274-2284 NN denotes expression
T1973 2285-2287 IN denotes of
T1974 2288-2294 NN denotes ADAM22
T1975 2295-2299 NN denotes mRNA
T1976 2300-2302 IN denotes in
T1977 2303-2306 DT denotes the
T1979 2307-2312 JJ denotes human
T1981 2313-2316 CC denotes and
T1980 2317-2322 NN denotes mouse
T1978 2323-2328 NN denotes brain
T1982 2329-2331 IN denotes by
T1983 2332-2340 NNP denotes Northern
T1984 2341-2345 NN denotes blot
T1985 2346-2354 NN denotes analysis
T1986 2355-2356 -LRB- denotes [
T1988 2356-2358 CD denotes 13
T1989 2358-2359 , denotes ,
T1987 2359-2361 CD denotes 14
T1990 2361-2362 -RRB- denotes ]
T1991 2362-2363 . denotes .
T1992 2363-2522 sentence denotes To determine the distribution of ADAM22 transcript in the adult mouse CNS precisely, in situ hybridisation analysis was performed using 35S-labeled RNA probe.
T1993 2364-2366 TO denotes To
T1994 2367-2376 VB denotes determine
T1996 2377-2380 DT denotes the
T1997 2381-2393 NN denotes distribution
T1998 2394-2396 IN denotes of
T1999 2397-2403 NN denotes ADAM22
T2000 2404-2414 NN denotes transcript
T2001 2415-2417 IN denotes in
T2002 2418-2421 DT denotes the
T2004 2422-2427 JJ denotes adult
T2005 2428-2433 NN denotes mouse
T2003 2434-2437 NN denotes CNS
T2006 2438-2447 RB denotes precisely
T2007 2447-2449 , denotes ,
T2008 2449-2451 FW denotes in
T2009 2452-2456 FW denotes situ
T2011 2457-2470 NN denotes hybridisation
T2010 2471-2479 NN denotes analysis
T2012 2480-2483 VBD denotes was
T1995 2484-2493 VBN denotes performed
T2013 2494-2499 VBG denotes using
T2014 2500-2503 CD denotes 35S
T2016 2503-2504 HYPH denotes -
T2015 2504-2511 VBN denotes labeled
T2018 2512-2515 NN denotes RNA
T2017 2516-2521 NN denotes probe
T2019 2521-2522 . denotes .
T2020 2522-2600 sentence denotes As shown in Fig. 3, ADAM22 mRNA was expressed throughout the adult mouse CNS.
T2021 2523-2525 IN denotes As
T2022 2526-2531 VBN denotes shown
T2024 2532-2534 IN denotes in
T2025 2535-2539 NN denotes Fig.
T2026 2540-2541 CD denotes 3
T2027 2541-2543 , denotes ,
T2028 2543-2549 NN denotes ADAM22
T2029 2550-2554 NN denotes mRNA
T2030 2555-2558 VBD denotes was
T2023 2559-2568 VBN denotes expressed
T2031 2569-2579 IN denotes throughout
T2032 2580-2583 DT denotes the
T2034 2584-2589 JJ denotes adult
T2035 2590-2595 NN denotes mouse
T2033 2596-2599 NN denotes CNS
T2036 2599-2600 . denotes .
T2037 2600-2686 sentence denotes Strong signal was detected in the cerebellar granule cells and hippocampal formation.
T2038 2601-2607 JJ denotes Strong
T2039 2608-2614 NN denotes signal
T2041 2615-2618 VBD denotes was
T2040 2619-2627 VBN denotes detected
T2042 2628-2630 IN denotes in
T2043 2631-2634 DT denotes the
T2045 2635-2645 JJ denotes cerebellar
T2046 2646-2653 NN denotes granule
T2044 2654-2659 NNS denotes cells
T2047 2660-2663 CC denotes and
T2048 2664-2675 JJ denotes hippocampal
T2049 2676-2685 NN denotes formation
T2050 2685-2686 . denotes .
T2051 2686-2762 sentence denotes In the spinal cord, hybridisation signal was restricted to the grey matter.
T2052 2687-2689 IN denotes In
T2054 2690-2693 DT denotes the
T2056 2694-2700 JJ denotes spinal
T2055 2701-2705 NN denotes cord
T2057 2705-2707 , denotes ,
T2058 2707-2720 NN denotes hybridisation
T2059 2721-2727 NN denotes signal
T2060 2728-2731 VBD denotes was
T2053 2732-2742 VBN denotes restricted
T2061 2743-2745 IN denotes to
T2062 2746-2749 DT denotes the
T2064 2750-2754 JJ denotes grey
T2063 2755-2761 NN denotes matter
T2065 2761-2762 . denotes .
T2066 2762-2901 sentence denotes The distribution of ADAM22 transcripts in the CNS was quite similar with that of ADAM11, whose neuronal expression has been reported [19].
T2067 2763-2766 DT denotes The
T2068 2767-2779 NN denotes distribution
T2070 2780-2782 IN denotes of
T2071 2783-2789 NN denotes ADAM22
T2072 2790-2801 NNS denotes transcripts
T2073 2802-2804 IN denotes in
T2074 2805-2808 DT denotes the
T2075 2809-2812 NN denotes CNS
T2069 2813-2816 VBD denotes was
T2076 2817-2822 RB denotes quite
T2077 2823-2830 JJ denotes similar
T2078 2831-2835 IN denotes with
T2079 2836-2840 DT denotes that
T2080 2841-2843 IN denotes of
T2081 2844-2850 NN denotes ADAM11
T2082 2850-2852 , denotes ,
T2083 2852-2857 WP$ denotes whose
T2085 2858-2866 JJ denotes neuronal
T2084 2867-2877 NN denotes expression
T2087 2878-2881 VBZ denotes has
T2088 2882-2886 VBN denotes been
T2086 2887-2895 VBN denotes reported
T2089 2896-2897 -LRB- denotes [
T2090 2897-2899 CD denotes 19
T2091 2899-2900 -RRB- denotes ]
T2092 2900-2901 . denotes .
T2093 2901-2985 sentence denotes These results suggest that the ADAM22 mRNA expression is neuronal in the mouse CNS.
T2094 2902-2907 DT denotes These
T2095 2908-2915 NNS denotes results
T2096 2916-2923 VBP denotes suggest
T2097 2924-2928 IN denotes that
T2099 2929-2932 DT denotes the
T2101 2933-2939 NN denotes ADAM22
T2102 2940-2944 NN denotes mRNA
T2100 2945-2955 NN denotes expression
T2098 2956-2958 VBZ denotes is
T2103 2959-2967 JJ denotes neuronal
T2104 2968-2970 IN denotes in
T2105 2971-2974 DT denotes the
T2107 2975-2980 NN denotes mouse
T2106 2981-2984 NN denotes CNS
T2108 2984-2985 . denotes .
T2109 2985-3118 sentence denotes Based on the mRNA distribution pattern, we performed immunohistopathological analysis of the cerebellum and hippocampus extensively.
T2110 2986-2991 VBN denotes Based
T2112 2992-2994 IN denotes on
T2113 2995-2998 DT denotes the
T2115 2999-3003 NN denotes mRNA
T2116 3004-3016 NN denotes distribution
T2114 3017-3024 NN denotes pattern
T2117 3024-3026 , denotes ,
T2118 3026-3028 PRP denotes we
T2111 3029-3038 VBD denotes performed
T2119 3039-3062 JJ denotes immunohistopathological
T2120 3063-3071 NN denotes analysis
T2121 3072-3074 IN denotes of
T2122 3075-3078 DT denotes the
T2123 3079-3089 NN denotes cerebellum
T2124 3090-3093 CC denotes and
T2125 3094-3105 NN denotes hippocampus
T2126 3106-3117 RB denotes extensively
T2127 3117-3118 . denotes .
T2128 3118-3341 sentence denotes Despite the high level of expression of ADAM22 mRNAs in the cerebellar granule cells, granule cell layer was normally formed and Purkinje cell morphology (calbindin-staining) of the mutant mouse looked intact (Figs. 4A–D).
T2129 3119-3126 IN denotes Despite
T2131 3127-3130 DT denotes the
T2133 3131-3135 JJ denotes high
T2132 3136-3141 NN denotes level
T2134 3142-3144 IN denotes of
T2135 3145-3155 NN denotes expression
T2136 3156-3158 IN denotes of
T2137 3159-3165 NN denotes ADAM22
T2138 3166-3171 NNS denotes mRNAs
T2139 3172-3174 IN denotes in
T2140 3175-3178 DT denotes the
T2142 3179-3189 JJ denotes cerebellar
T2143 3190-3197 NN denotes granule
T2141 3198-3203 NNS denotes cells
T2144 3203-3205 , denotes ,
T2145 3205-3212 NN denotes granule
T2147 3213-3217 NN denotes cell
T2146 3218-3223 NN denotes layer
T2148 3224-3227 VBD denotes was
T2149 3228-3236 RB denotes normally
T2130 3237-3243 VBN denotes formed
T2150 3244-3247 CC denotes and
T2151 3248-3256 NNP denotes Purkinje
T2152 3257-3261 NN denotes cell
T2153 3262-3272 NN denotes morphology
T2155 3273-3274 -LRB- denotes (
T2157 3274-3283 NN denotes calbindin
T2158 3283-3284 HYPH denotes -
T2156 3284-3292 VBG denotes staining
T2159 3292-3293 -RRB- denotes )
T2160 3294-3296 IN denotes of
T2161 3297-3300 DT denotes the
T2163 3301-3307 NN denotes mutant
T2162 3308-3313 NN denotes mouse
T2154 3314-3320 VBD denotes looked
T2164 3321-3327 JJ denotes intact
T2165 3328-3329 -LRB- denotes (
T2167 3329-3334 NNS denotes Figs.
T2166 3335-3337 NN denotes 4A
T2168 3337-3338 SYM denotes –
T2169 3338-3339 NN denotes D
T2170 3339-3340 -RRB- denotes )
T2171 3340-3341 . denotes .
T2172 3341-3402 sentence denotes Hippocampal formation was also normally formed (Figs. 4I–J).
T2173 3342-3353 JJ denotes Hippocampal
T2174 3354-3363 NN denotes formation
T2176 3364-3367 VBD denotes was
T2177 3368-3372 RB denotes also
T2178 3373-3381 RB denotes normally
T2175 3382-3388 VBN denotes formed
T2179 3389-3390 -LRB- denotes (
T2181 3390-3395 NNS denotes Figs.
T2180 3396-3398 NN denotes 4I
T2182 3398-3399 SYM denotes –
T2183 3399-3400 NN denotes J
T2184 3400-3401 -RRB- denotes )
T2185 3401-3402 . denotes .
T2186 3402-3491 sentence denotes These results suggest that neuronal cell migration was not impaired in the mutant mouse.
T2187 3403-3408 DT denotes These
T2188 3409-3416 NNS denotes results
T2189 3417-3424 VBP denotes suggest
T2190 3425-3429 IN denotes that
T2192 3430-3438 JJ denotes neuronal
T2193 3439-3443 NN denotes cell
T2194 3444-3453 NN denotes migration
T2195 3454-3457 VBD denotes was
T2196 3458-3461 RB denotes not
T2191 3462-3470 VBN denotes impaired
T2197 3471-3473 IN denotes in
T2198 3474-3477 DT denotes the
T2200 3478-3484 NN denotes mutant
T2199 3485-3490 NN denotes mouse
T2201 3490-3491 . denotes .
T2202 3491-3695 sentence denotes Myelin-formation detected by MBP (myelin basic protein) staining of the mutant in the cerebellum (Fig. 4H) and spinal cord (Fig. 4L) was also indistinguishable with the wild-type littermate (Figs. 4G,K).
T2203 3492-3498 NN denotes Myelin
T2205 3498-3499 HYPH denotes -
T2204 3499-3508 NN denotes formation
T2207 3509-3517 VBN denotes detected
T2208 3518-3520 IN denotes by
T2209 3521-3524 NN denotes MBP
T2211 3525-3526 -LRB- denotes (
T2212 3526-3532 NN denotes myelin
T2214 3533-3538 JJ denotes basic
T2213 3539-3546 NN denotes protein
T2215 3546-3547 -RRB- denotes )
T2210 3548-3556 NN denotes staining
T2216 3557-3559 IN denotes of
T2217 3560-3563 DT denotes the
T2218 3564-3570 NN denotes mutant
T2219 3571-3573 IN denotes in
T2220 3574-3577 DT denotes the
T2221 3578-3588 NN denotes cerebellum
T2222 3589-3590 -LRB- denotes (
T2224 3590-3594 NN denotes Fig.
T2223 3595-3597 NN denotes 4H
T2225 3597-3598 -RRB- denotes )
T2226 3599-3602 CC denotes and
T2227 3603-3609 JJ denotes spinal
T2228 3610-3614 NN denotes cord
T2229 3615-3616 -LRB- denotes (
T2231 3616-3620 NN denotes Fig.
T2230 3621-3623 NN denotes 4L
T2232 3623-3624 -RRB- denotes )
T2206 3625-3628 VBD denotes was
T2233 3629-3633 RB denotes also
T2234 3634-3651 JJ denotes indistinguishable
T2235 3652-3656 IN denotes with
T2236 3657-3660 DT denotes the
T2238 3661-3665 JJ denotes wild
T2240 3665-3666 HYPH denotes -
T2239 3666-3670 NN denotes type
T2237 3671-3681 NN denotes littermate
T2241 3682-3683 -LRB- denotes (
T2243 3683-3688 NNS denotes Figs.
T2244 3689-3691 NN denotes 4G
T2245 3691-3692 , denotes ,
T2242 3692-3693 NN denotes K
T2246 3693-3694 -RRB- denotes )
T2247 3694-3695 . denotes .
T2248 3695-3816 sentence denotes In summary, we could not find any signs of abnormalities in the mutant mouse brain by the light microscopic examination.
T2249 3696-3698 IN denotes In
T2251 3699-3706 NN denotes summary
T2252 3706-3708 , denotes ,
T2253 3708-3710 PRP denotes we
T2254 3711-3716 MD denotes could
T2255 3717-3720 RB denotes not
T2250 3721-3725 VB denotes find
T2256 3726-3729 DT denotes any
T2257 3730-3735 NNS denotes signs
T2258 3736-3738 IN denotes of
T2259 3739-3752 NNS denotes abnormalities
T2260 3753-3755 IN denotes in
T2261 3756-3759 DT denotes the
T2263 3760-3766 NN denotes mutant
T2264 3767-3772 NN denotes mouse
T2262 3773-3778 NN denotes brain
T2265 3779-3781 IN denotes by
T2266 3782-3785 DT denotes the
T2268 3786-3791 NN denotes light
T2269 3792-3803 JJ denotes microscopic
T2267 3804-3815 NN denotes examination
T2270 3815-3816 . denotes .
T2271 3816-3948 sentence denotes Next, the spinal cord and peripheral nerves of each genotype were analysed by toluidine blue stain, which reveals myelin formation.
T2272 3817-3821 RB denotes Next
T2274 3821-3823 , denotes ,
T2275 3823-3826 DT denotes the
T2277 3827-3833 JJ denotes spinal
T2276 3834-3838 NN denotes cord
T2278 3839-3842 CC denotes and
T2279 3843-3853 JJ denotes peripheral
T2280 3854-3860 NNS denotes nerves
T2281 3861-3863 IN denotes of
T2282 3864-3868 DT denotes each
T2283 3869-3877 NN denotes genotype
T2284 3878-3882 VBD denotes were
T2273 3883-3891 VBN denotes analysed
T2285 3892-3894 IN denotes by
T2286 3895-3904 NN denotes toluidine
T2288 3905-3909 JJ denotes blue
T2287 3910-3915 NN denotes stain
T2289 3915-3917 , denotes ,
T2290 3917-3922 WDT denotes which
T2291 3923-3930 VBZ denotes reveals
T2292 3931-3937 NN denotes myelin
T2293 3938-3947 NN denotes formation
T2294 3947-3948 . denotes .
T2295 3948-4092 sentence denotes Surprisingly, lack of myelin or thin myelin was observed in the sciatic nerves (Fig. 5B) and trigeminal nerves (Fig. 5D) in homozygous mutants.
T2296 3949-3961 RB denotes Surprisingly
T2298 3961-3963 , denotes ,
T2299 3963-3967 NN denotes lack
T2300 3968-3970 IN denotes of
T2301 3971-3977 NN denotes myelin
T2302 3978-3980 CC denotes or
T2303 3981-3985 JJ denotes thin
T2304 3986-3992 NN denotes myelin
T2305 3993-3996 VBD denotes was
T2297 3997-4005 VBN denotes observed
T2306 4006-4008 IN denotes in
T2307 4009-4012 DT denotes the
T2309 4013-4020 JJ denotes sciatic
T2308 4021-4027 NNS denotes nerves
T2310 4028-4029 -LRB- denotes (
T2312 4029-4033 NN denotes Fig.
T2311 4034-4036 NN denotes 5B
T2313 4036-4037 -RRB- denotes )
T2314 4038-4041 CC denotes and
T2315 4042-4052 JJ denotes trigeminal
T2316 4053-4059 NNS denotes nerves
T2317 4060-4061 -LRB- denotes (
T2319 4061-4065 NN denotes Fig.
T2318 4066-4068 NN denotes 5D
T2320 4068-4069 -RRB- denotes )
T2321 4070-4072 IN denotes in
T2322 4073-4083 JJ denotes homozygous
T2323 4084-4091 NNS denotes mutants
T2324 4091-4092 . denotes .
T2325 4092-4251 sentence denotes Because no lesions were observed in the spinal cord (Fig. 5F), it was suggested that Schwann cell specific myelination defect occurred in the homozygous mice.
T2326 4093-4100 IN denotes Because
T2328 4101-4103 DT denotes no
T2329 4104-4111 NNS denotes lesions
T2330 4112-4116 VBD denotes were
T2327 4117-4125 VBN denotes observed
T2332 4126-4128 IN denotes in
T2333 4129-4132 DT denotes the
T2335 4133-4139 JJ denotes spinal
T2334 4140-4144 NN denotes cord
T2336 4145-4146 -LRB- denotes (
T2338 4146-4150 NN denotes Fig.
T2337 4151-4153 NN denotes 5F
T2339 4153-4154 -RRB- denotes )
T2340 4154-4156 , denotes ,
T2341 4156-4158 PRP denotes it
T2342 4159-4162 VBD denotes was
T2331 4163-4172 VBN denotes suggested
T2343 4173-4177 IN denotes that
T2345 4178-4185 NNP denotes Schwann
T2346 4186-4190 NN denotes cell
T2347 4191-4199 JJ denotes specific
T2349 4200-4211 NN denotes myelination
T2348 4212-4218 NN denotes defect
T2344 4219-4227 VBD denotes occurred
T2350 4228-4230 IN denotes in
T2351 4231-4234 DT denotes the
T2353 4235-4245 JJ denotes homozygous
T2352 4246-4250 NNS denotes mice
T2354 4250-4251 . denotes .
T2355 4251-4392 sentence denotes To analyse the state of myelinating Schwann cells and axons, electron microscopic (EM) analysis of the sciatic nerve was performed (Fig. 6).
T2356 4252-4254 TO denotes To
T2357 4255-4262 VB denotes analyse
T2359 4263-4266 DT denotes the
T2360 4267-4272 NN denotes state
T2361 4273-4275 IN denotes of
T2362 4276-4287 VBG denotes myelinating
T2364 4288-4295 NNP denotes Schwann
T2363 4296-4301 NNS denotes cells
T2365 4302-4305 CC denotes and
T2366 4306-4311 NNS denotes axons
T2367 4311-4313 , denotes ,
T2368 4313-4321 NN denotes electron
T2369 4322-4333 JJ denotes microscopic
T2371 4334-4335 -LRB- denotes (
T2372 4335-4337 JJ denotes EM
T2373 4337-4338 -RRB- denotes )
T2370 4339-4347 NN denotes analysis
T2374 4348-4350 IN denotes of
T2375 4351-4354 DT denotes the
T2377 4355-4362 JJ denotes sciatic
T2376 4363-4368 NN denotes nerve
T2378 4369-4372 VBD denotes was
T2358 4373-4382 VBN denotes performed
T2379 4383-4384 -LRB- denotes (
T2380 4384-4388 NN denotes Fig.
T2381 4389-4390 CD denotes 6
T2382 4390-4391 -RRB- denotes )
T2383 4391-4392 . denotes .
T2384 4392-4467 sentence denotes Schwann cells formed thin or no myelin in the homozygous mutant (Fig. 6B).
T2385 4393-4400 NNP denotes Schwann
T2386 4401-4406 NNS denotes cells
T2387 4407-4413 VBD denotes formed
T2388 4414-4418 JJ denotes thin
T2389 4419-4421 CC denotes or
T2390 4422-4424 DT denotes no
T2391 4425-4431 NN denotes myelin
T2392 4432-4434 IN denotes in
T2393 4435-4438 DT denotes the
T2395 4439-4449 JJ denotes homozygous
T2394 4450-4456 NN denotes mutant
T2396 4457-4458 -LRB- denotes (
T2398 4458-4462 NN denotes Fig.
T2397 4463-4465 NN denotes 6B
T2399 4465-4466 -RRB- denotes )
T2400 4466-4467 . denotes .
T2401 4467-4537 sentence denotes In contrast, heterozygous mice showed complete myelination (Fig. 6A).
T2402 4468-4470 IN denotes In
T2404 4471-4479 NN denotes contrast
T2405 4479-4481 , denotes ,
T2406 4481-4493 JJ denotes heterozygous
T2407 4494-4498 NNS denotes mice
T2403 4499-4505 VBD denotes showed
T2408 4506-4514 JJ denotes complete
T2409 4515-4526 NN denotes myelination
T2410 4527-4528 -LRB- denotes (
T2412 4528-4532 NN denotes Fig.
T2411 4533-4535 NN denotes 6A
T2413 4535-4536 -RRB- denotes )
T2414 4536-4537 . denotes .
T2415 4537-4593 sentence denotes Morphology of the axons looked normal in each genotype.
T2416 4538-4548 NN denotes Morphology
T2418 4549-4551 IN denotes of
T2419 4552-4555 DT denotes the
T2420 4556-4561 NNS denotes axons
T2417 4562-4568 VBD denotes looked
T2421 4569-4575 JJ denotes normal
T2422 4576-4578 IN denotes in
T2423 4579-4583 DT denotes each
T2424 4584-4592 NN denotes genotype
T2425 4592-4593 . denotes .
T2426 4593-4772 sentence denotes Immunohistochemical analysis of the sciatic nerve showed that increased number of nuclei (Fig. 7B) and reduced staining of MBP (Fig. 7D) were remarkable in the homozygous mutant.
T2427 4594-4613 JJ denotes Immunohistochemical
T2428 4614-4622 NN denotes analysis
T2430 4623-4625 IN denotes of
T2431 4626-4629 DT denotes the
T2433 4630-4637 JJ denotes sciatic
T2432 4638-4643 NN denotes nerve
T2429 4644-4650 VBD denotes showed
T2434 4651-4655 IN denotes that
T2436 4656-4665 VBN denotes increased
T2437 4666-4672 NN denotes number
T2438 4673-4675 IN denotes of
T2439 4676-4682 NNS denotes nuclei
T2440 4683-4684 -LRB- denotes (
T2442 4684-4688 NN denotes Fig.
T2441 4689-4691 NN denotes 7B
T2443 4691-4692 -RRB- denotes )
T2444 4693-4696 CC denotes and
T2445 4697-4704 VBN denotes reduced
T2446 4705-4713 NN denotes staining
T2447 4714-4716 IN denotes of
T2448 4717-4720 NN denotes MBP
T2449 4721-4722 -LRB- denotes (
T2451 4722-4726 NN denotes Fig.
T2450 4727-4729 NN denotes 7D
T2452 4729-4730 -RRB- denotes )
T2435 4731-4735 VBD denotes were
T2453 4736-4746 JJ denotes remarkable
T2454 4747-4749 IN denotes in
T2455 4750-4753 DT denotes the
T2457 4754-4764 JJ denotes homozygous
T2456 4765-4771 NN denotes mutant
T2458 4771-4772 . denotes .
T2459 4772-4895 sentence denotes The Schwann cell marker, S100 staining signal was intensely observed in the homozygote as well as wild-type (Figs. 7E, F).
T2460 4773-4776 DT denotes The
T2462 4777-4784 NNP denotes Schwann
T2463 4785-4789 NN denotes cell
T2461 4790-4796 NN denotes marker
T2465 4796-4798 , denotes ,
T2466 4798-4802 NN denotes S100
T2468 4803-4811 NN denotes staining
T2467 4812-4818 NN denotes signal
T2469 4819-4822 VBD denotes was
T2470 4823-4832 RB denotes intensely
T2464 4833-4841 VBN denotes observed
T2471 4842-4844 IN denotes in
T2472 4845-4848 DT denotes the
T2473 4849-4859 NN denotes homozygote
T2474 4860-4862 RB denotes as
T2476 4863-4867 RB denotes well
T2475 4868-4870 IN denotes as
T2477 4871-4875 JJ denotes wild
T2479 4875-4876 HYPH denotes -
T2478 4876-4880 NN denotes type
T2480 4881-4882 -LRB- denotes (
T2482 4882-4887 NNS denotes Figs.
T2483 4888-4890 NN denotes 7E
T2484 4890-4892 , denotes ,
T2481 4892-4893 NN denotes F
T2485 4893-4894 -RRB- denotes )
T2486 4894-4895 . denotes .
T2487 4895-5044 sentence denotes These results suggest that proliferation of the Schwann cells was not impaired but differentiation is severely delayed in the ADAM22-deficient mice.
T2488 4896-4901 DT denotes These
T2489 4902-4909 NNS denotes results
T2490 4910-4917 VBP denotes suggest
T2491 4918-4922 IN denotes that
T2493 4923-4936 NN denotes proliferation
T2494 4937-4939 IN denotes of
T2495 4940-4943 DT denotes the
T2497 4944-4951 NNP denotes Schwann
T2496 4952-4957 NNS denotes cells
T2492 4958-4961 VBD denotes was
T2498 4962-4965 RB denotes not
T2499 4966-4974 JJ denotes impaired
T2500 4975-4978 CC denotes but
T2501 4979-4994 NN denotes differentiation
T2502 4995-4997 VBZ denotes is
T2503 4998-5006 RB denotes severely
T2504 5007-5014 JJ denotes delayed
T2505 5015-5017 IN denotes in
T2506 5018-5021 DT denotes the
T2508 5022-5028 NN denotes ADAM22
T2510 5028-5029 HYPH denotes -
T2509 5029-5038 JJ denotes deficient
T2507 5039-5043 NNS denotes mice
T2511 5043-5044 . denotes .
T2746 5046-5051 JJ denotes Novel
T2748 5052-5058 NN denotes ADAM22
T2749 5059-5069 NN denotes transcript
T2747 5070-5078 NNS denotes variants
T2750 5079-5087 VBN denotes isolated
T2751 5088-5092 IN denotes from
T2752 5093-5096 DT denotes the
T2754 5097-5107 JJ denotes peripheral
T2755 5108-5115 JJ denotes nervous
T2753 5116-5122 NN denotes system
T2756 5122-5257 sentence denotes In the case of human ADAM22, isolation of five splicing variants and existence of two terminating exons have been reported [13,20,21].
T2757 5123-5125 IN denotes In
T2759 5126-5129 DT denotes the
T2760 5130-5134 NN denotes case
T2761 5135-5137 IN denotes of
T2762 5138-5143 JJ denotes human
T2763 5144-5150 NN denotes ADAM22
T2764 5150-5152 , denotes ,
T2765 5152-5161 NN denotes isolation
T2766 5162-5164 IN denotes of
T2767 5165-5169 CD denotes five
T2769 5170-5178 NN denotes splicing
T2768 5179-5187 NNS denotes variants
T2770 5188-5191 CC denotes and
T2771 5192-5201 NN denotes existence
T2772 5202-5204 IN denotes of
T2773 5205-5208 CD denotes two
T2775 5209-5220 VBG denotes terminating
T2774 5221-5226 NNS denotes exons
T2776 5227-5231 VBP denotes have
T2777 5232-5236 VBN denotes been
T2758 5237-5245 VBN denotes reported
T2778 5246-5247 -LRB- denotes [
T2780 5247-5249 CD denotes 13
T2781 5249-5250 , denotes ,
T2782 5250-5252 CD denotes 20
T2783 5252-5253 , denotes ,
T2779 5253-5255 CD denotes 21
T2784 5255-5256 -RRB- denotes ]
T2785 5256-5257 . denotes .
T2786 5257-5354 sentence denotes However, the latter terminating exon (exon 31) of the mouse species has not been identified yet.
T2787 5258-5265 RB denotes However
T2789 5265-5267 , denotes ,
T2790 5267-5270 DT denotes the
T2792 5271-5277 JJ denotes latter
T2793 5278-5289 VBG denotes terminating
T2791 5290-5294 NN denotes exon
T2794 5295-5296 -LRB- denotes (
T2795 5296-5300 NN denotes exon
T2796 5301-5303 CD denotes 31
T2797 5303-5304 -RRB- denotes )
T2798 5305-5307 IN denotes of
T2799 5308-5311 DT denotes the
T2801 5312-5317 NN denotes mouse
T2800 5318-5325 NNS denotes species
T2802 5326-5329 VBZ denotes has
T2803 5330-5333 RB denotes not
T2804 5334-5338 VBN denotes been
T2788 5339-5349 VBN denotes identified
T2805 5350-5353 RB denotes yet
T2806 5353-5354 . denotes .
T2807 5354-5471 sentence denotes To isolate the long form of mouse ADAM22 transcripts, at first, we sought the mouse genome by BLAST homology search.
T2808 5355-5357 TO denotes To
T2809 5358-5365 VB denotes isolate
T2811 5366-5369 DT denotes the
T2813 5370-5374 JJ denotes long
T2812 5375-5379 NN denotes form
T2814 5380-5382 IN denotes of
T2815 5383-5388 NN denotes mouse
T2816 5389-5395 NN denotes ADAM22
T2817 5396-5407 NNS denotes transcripts
T2818 5407-5409 , denotes ,
T2819 5409-5411 IN denotes at
T2820 5412-5417 RB denotes first
T2821 5417-5419 , denotes ,
T2822 5419-5421 PRP denotes we
T2810 5422-5428 VBD denotes sought
T2823 5429-5432 DT denotes the
T2825 5433-5438 NN denotes mouse
T2824 5439-5445 NN denotes genome
T2826 5446-5448 IN denotes by
T2827 5449-5454 NNP denotes BLAST
T2828 5455-5463 NN denotes homology
T2829 5464-5470 NN denotes search
T2830 5470-5471 . denotes .
T2831 5471-5573 sentence denotes Search results showed that the mouse genome contig NT_039297 contained most of the mouse Adam22 gene.
T2832 5472-5478 NN denotes Search
T2833 5479-5486 NNS denotes results
T2834 5487-5493 VBD denotes showed
T2835 5494-5498 IN denotes that
T2837 5499-5502 DT denotes the
T2839 5503-5508 NN denotes mouse
T2838 5509-5515 NN denotes genome
T2840 5516-5522 NN denotes contig
T2841 5523-5532 NN denotes NT_039297
T2836 5533-5542 VBD denotes contained
T2842 5543-5547 JJS denotes most
T2843 5548-5550 IN denotes of
T2844 5551-5554 DT denotes the
T2846 5555-5560 NN denotes mouse
T2847 5561-5567 NN denotes Adam22
T2845 5568-5572 NN denotes gene
T2848 5572-5573 . denotes .
T2849 5573-5717 sentence denotes In this contig sequence, we found putative exons 30 and 31, those sequences were quite similar to human ADAM22 isoform 1 (GenBank # NM_021723).
T2850 5574-5576 IN denotes In
T2852 5577-5581 DT denotes this
T2854 5582-5588 NN denotes contig
T2853 5589-5597 NN denotes sequence
T2855 5597-5599 , denotes ,
T2856 5599-5601 PRP denotes we
T2851 5602-5607 VBD denotes found
T2858 5608-5616 JJ denotes putative
T2860 5617-5622 NNS denotes exons
T2859 5623-5625 CD denotes 30
T2861 5626-5629 CC denotes and
T2862 5630-5632 CD denotes 31
T2863 5632-5634 , denotes ,
T2864 5634-5639 DT denotes those
T2865 5640-5649 NNS denotes sequences
T2857 5650-5654 VBD denotes were
T2866 5655-5660 RB denotes quite
T2867 5661-5668 JJ denotes similar
T2868 5669-5671 IN denotes to
T2869 5672-5677 JJ denotes human
T2871 5678-5684 NN denotes ADAM22
T2870 5685-5692 NN denotes isoform
T2872 5693-5694 CD denotes 1
T2873 5695-5696 -LRB- denotes (
T2875 5696-5703 NNP denotes GenBank
T2876 5704-5705 SYM denotes #
T2874 5706-5715 NN denotes NM_021723
T2877 5715-5716 -RRB- denotes )
T2878 5716-5717 . denotes .
T2879 5717-5862 sentence denotes To confirm existence of transcripts, we designed primers on the putative exon 31 and performed RT-PCR using mouse cerebellum mRNA as a template.
T2880 5718-5720 TO denotes To
T2881 5721-5728 VB denotes confirm
T2883 5729-5738 NN denotes existence
T2884 5739-5741 IN denotes of
T2885 5742-5753 NNS denotes transcripts
T2886 5753-5755 , denotes ,
T2887 5755-5757 PRP denotes we
T2882 5758-5766 VBD denotes designed
T2888 5767-5774 NNS denotes primers
T2889 5775-5777 IN denotes on
T2890 5778-5781 DT denotes the
T2892 5782-5790 JJ denotes putative
T2891 5791-5795 NN denotes exon
T2893 5796-5798 CD denotes 31
T2894 5799-5802 CC denotes and
T2895 5803-5812 VBN denotes performed
T2896 5813-5815 NN denotes RT
T2898 5815-5816 HYPH denotes -
T2897 5816-5819 NN denotes PCR
T2899 5820-5825 VBG denotes using
T2900 5826-5831 NN denotes mouse
T2902 5832-5842 NN denotes cerebellum
T2901 5843-5847 NN denotes mRNA
T2903 5848-5850 IN denotes as
T2904 5851-5852 DT denotes a
T2905 5853-5861 NN denotes template
T2906 5861-5862 . denotes .
T2907 5862-6078 sentence denotes TA-cloning of the amplified fragments and sequencing analysis showed that 5 of 8 clones were type 1 isoform (G01), 2 clones were type 3 isoform (G03) and 1 clone (G08) contained a novel exon between exons 29 and 30.
T2908 5863-5865 NN denotes TA
T2910 5865-5866 HYPH denotes -
T2909 5866-5873 NN denotes cloning
T2912 5874-5876 IN denotes of
T2913 5877-5880 DT denotes the
T2915 5881-5890 VBN denotes amplified
T2914 5891-5900 NNS denotes fragments
T2916 5901-5904 CC denotes and
T2917 5905-5915 NN denotes sequencing
T2918 5916-5924 NN denotes analysis
T2911 5925-5931 VBD denotes showed
T2919 5932-5936 IN denotes that
T2921 5937-5938 CD denotes 5
T2923 5939-5941 IN denotes of
T2922 5942-5943 CD denotes 8
T2924 5944-5950 NNS denotes clones
T2920 5951-5955 VBD denotes were
T2925 5956-5960 NN denotes type
T2927 5961-5962 CD denotes 1
T2926 5963-5970 NN denotes isoform
T2928 5971-5972 -LRB- denotes (
T2929 5972-5975 NN denotes G01
T2930 5975-5976 -RRB- denotes )
T2931 5976-5978 , denotes ,
T2932 5978-5979 CD denotes 2
T2933 5980-5986 NNS denotes clones
T2934 5987-5991 VBD denotes were
T2935 5992-5996 NN denotes type
T2937 5997-5998 CD denotes 3
T2936 5999-6006 NN denotes isoform
T2938 6007-6008 -LRB- denotes (
T2939 6008-6011 NN denotes G03
T2940 6011-6012 -RRB- denotes )
T2941 6013-6016 CC denotes and
T2942 6017-6018 CD denotes 1
T2943 6019-6024 NN denotes clone
T2945 6025-6026 -LRB- denotes (
T2946 6026-6029 NN denotes G08
T2947 6029-6030 -RRB- denotes )
T2944 6031-6040 VBD denotes contained
T2948 6041-6042 DT denotes a
T2950 6043-6048 JJ denotes novel
T2949 6049-6053 NN denotes exon
T2951 6054-6061 IN denotes between
T2952 6062-6067 NNS denotes exons
T2953 6068-6070 CD denotes 29
T2954 6071-6074 CC denotes and
T2955 6075-6077 CD denotes 30
T2956 6077-6078 . denotes .
T2957 6078-6268 sentence denotes Next, we performed RT-PCR analysis of mRNAs purified from several adult mouse tissues, such as the spinal cord, dorsal root ganglion (DRG), sciatic nerve and cultured primary Schwann cells.
T2958 6079-6083 RB denotes Next
T2960 6083-6085 , denotes ,
T2961 6085-6087 PRP denotes we
T2959 6088-6097 VBD denotes performed
T2962 6098-6100 NN denotes RT
T2964 6100-6101 HYPH denotes -
T2963 6101-6104 NN denotes PCR
T2965 6105-6113 NN denotes analysis
T2966 6114-6116 IN denotes of
T2967 6117-6122 NNS denotes mRNAs
T2968 6123-6131 VBN denotes purified
T2969 6132-6136 IN denotes from
T2970 6137-6144 JJ denotes several
T2972 6145-6150 JJ denotes adult
T2973 6151-6156 NN denotes mouse
T2971 6157-6164 NNS denotes tissues
T2974 6164-6166 , denotes ,
T2975 6166-6170 JJ denotes such
T2976 6171-6173 IN denotes as
T2977 6174-6177 DT denotes the
T2979 6178-6184 JJ denotes spinal
T2978 6185-6189 NN denotes cord
T2980 6189-6191 , denotes ,
T2981 6191-6197 JJ denotes dorsal
T2982 6198-6202 NN denotes root
T2983 6203-6211 NN denotes ganglion
T2984 6212-6213 -LRB- denotes (
T2985 6213-6216 NN denotes DRG
T2986 6216-6217 -RRB- denotes )
T2987 6217-6219 , denotes ,
T2988 6219-6226 JJ denotes sciatic
T2989 6227-6232 NN denotes nerve
T2990 6233-6236 CC denotes and
T2991 6237-6245 VBN denotes cultured
T2993 6246-6253 JJ denotes primary
T2994 6254-6261 NNP denotes Schwann
T2992 6262-6267 NNS denotes cells
T2995 6267-6268 . denotes .
T2996 6268-6406 sentence denotes Interestingly, multiple DNA fragments were amplified from each tissue (Fig. 8), and length of major fragments in each lane was different.
T2997 6269-6282 RB denotes Interestingly
T2999 6282-6284 , denotes ,
T3000 6284-6292 JJ denotes multiple
T3002 6293-6296 NN denotes DNA
T3001 6297-6306 NNS denotes fragments
T3003 6307-6311 VBD denotes were
T2998 6312-6321 VBN denotes amplified
T3004 6322-6326 IN denotes from
T3005 6327-6331 DT denotes each
T3006 6332-6338 NN denotes tissue
T3007 6339-6340 -LRB- denotes (
T3008 6340-6344 NN denotes Fig.
T3009 6345-6346 CD denotes 8
T3010 6346-6347 -RRB- denotes )
T3011 6347-6349 , denotes ,
T3012 6349-6352 CC denotes and
T3013 6353-6359 NN denotes length
T3015 6360-6362 IN denotes of
T3016 6363-6368 JJ denotes major
T3017 6369-6378 NNS denotes fragments
T3018 6379-6381 IN denotes in
T3019 6382-6386 DT denotes each
T3020 6387-6391 NN denotes lane
T3014 6392-6395 VBD denotes was
T3021 6396-6405 JJ denotes different
T3022 6405-6406 . denotes .
T3023 6406-6607 sentence denotes For example, length of major amplified PCR fragments from the sciatic nerve (Fig. 8A, lane 5) and cultured Schwann cells (lane 6) were almost identical, and were shorter than those from other tissues.
T3024 6407-6410 IN denotes For
T3026 6411-6418 NN denotes example
T3027 6418-6420 , denotes ,
T3028 6420-6426 NN denotes length
T3029 6427-6429 IN denotes of
T3030 6430-6435 JJ denotes major
T3032 6436-6445 VBN denotes amplified
T3033 6446-6449 NN denotes PCR
T3031 6450-6459 NNS denotes fragments
T3034 6460-6464 IN denotes from
T3035 6465-6468 DT denotes the
T3037 6469-6476 JJ denotes sciatic
T3036 6477-6482 NN denotes nerve
T3038 6483-6484 -LRB- denotes (
T3040 6484-6488 NN denotes Fig.
T3041 6489-6491 NN denotes 8A
T3042 6491-6493 , denotes ,
T3039 6493-6497 NN denotes lane
T3043 6498-6499 CD denotes 5
T3044 6499-6500 -RRB- denotes )
T3045 6501-6504 CC denotes and
T3046 6505-6513 VBN denotes cultured
T3048 6514-6521 NNP denotes Schwann
T3047 6522-6527 NNS denotes cells
T3049 6528-6529 -LRB- denotes (
T3050 6529-6533 NN denotes lane
T3051 6534-6535 CD denotes 6
T3052 6535-6536 -RRB- denotes )
T3025 6537-6541 VBD denotes were
T3053 6542-6548 RB denotes almost
T3054 6549-6558 JJ denotes identical
T3055 6558-6560 , denotes ,
T3056 6560-6563 CC denotes and
T3057 6564-6568 VBD denotes were
T3058 6569-6576 JJR denotes shorter
T3059 6577-6581 IN denotes than
T3060 6582-6587 DT denotes those
T3061 6588-6592 IN denotes from
T3062 6593-6598 JJ denotes other
T3063 6599-6606 NNS denotes tissues
T3064 6606-6607 . denotes .
T3065 6607-6683 sentence denotes In contrast, major fragments from the DRG (lane 4) were longer than others.
T3066 6608-6610 IN denotes In
T3068 6611-6619 NN denotes contrast
T3069 6619-6621 , denotes ,
T3070 6621-6626 JJ denotes major
T3071 6627-6636 NNS denotes fragments
T3072 6637-6641 IN denotes from
T3073 6642-6645 DT denotes the
T3074 6646-6649 NN denotes DRG
T3075 6650-6651 -LRB- denotes (
T3076 6651-6655 NN denotes lane
T3077 6656-6657 CD denotes 4
T3078 6657-6658 -RRB- denotes )
T3067 6659-6663 VBD denotes were
T3079 6664-6670 JJR denotes longer
T3080 6671-6675 IN denotes than
T3081 6676-6682 NNS denotes others
T3082 6682-6683 . denotes .
T3083 6683-6791 sentence denotes We performed TA-cloning and sequencing to analyse exon organization of the amplified transcripts (Fig. 8C).
T3084 6684-6686 PRP denotes We
T3085 6687-6696 VBD denotes performed
T3086 6697-6699 NN denotes TA
T3087 6699-6700 HYPH denotes -
T3088 6700-6707 VBG denotes cloning
T3089 6708-6711 CC denotes and
T3090 6712-6722 NN denotes sequencing
T3091 6723-6725 TO denotes to
T3092 6726-6733 VB denotes analyse
T3093 6734-6738 NN denotes exon
T3094 6739-6751 NN denotes organization
T3095 6752-6754 IN denotes of
T3096 6755-6758 DT denotes the
T3098 6759-6768 VBN denotes amplified
T3097 6769-6780 NNS denotes transcripts
T3099 6781-6782 -LRB- denotes (
T3101 6782-6786 NN denotes Fig.
T3100 6787-6789 NN denotes 8C
T3102 6789-6790 -RRB- denotes )
T3103 6790-6791 . denotes .
T3104 6791-6891 sentence denotes Forty seven clones were isolated in total and were classified in 16 independent clones (G01 – G23).
T3105 6792-6797 CD denotes Forty
T3106 6798-6803 CD denotes seven
T3107 6804-6810 NNS denotes clones
T3109 6811-6815 VBD denotes were
T3108 6816-6824 VBN denotes isolated
T3110 6825-6827 IN denotes in
T3111 6828-6833 JJ denotes total
T3112 6834-6837 CC denotes and
T3113 6838-6842 VBD denotes were
T3114 6843-6853 VBN denotes classified
T3115 6854-6856 IN denotes in
T3116 6857-6859 CD denotes 16
T3118 6860-6871 JJ denotes independent
T3117 6872-6878 NNS denotes clones
T3119 6879-6880 -LRB- denotes (
T3120 6880-6883 NN denotes G01
T3121 6884-6885 SYM denotes –
T3122 6886-6889 NN denotes G23
T3123 6889-6890 -RRB- denotes )
T3124 6890-6891 . denotes .
T3125 6891-7021 sentence denotes Comparison with the genomic sequence revealed the existence of 8 novel exons (exons 27S, 27L, 29.1, 29.3, 29.5, 29.7, 30 and 31).
T3126 6892-6902 NN denotes Comparison
T3128 6903-6907 IN denotes with
T3129 6908-6911 DT denotes the
T3131 6912-6919 JJ denotes genomic
T3130 6920-6928 NN denotes sequence
T3127 6929-6937 VBD denotes revealed
T3132 6938-6941 DT denotes the
T3133 6942-6951 NN denotes existence
T3134 6952-6954 IN denotes of
T3135 6955-6956 CD denotes 8
T3137 6957-6962 JJ denotes novel
T3136 6963-6968 NNS denotes exons
T3138 6969-6970 -LRB- denotes (
T3139 6970-6975 NNS denotes exons
T3140 6976-6979 NN denotes 27S
T3141 6979-6981 , denotes ,
T3142 6981-6984 NN denotes 27L
T3143 6984-6986 , denotes ,
T3144 6986-6990 CD denotes 29.1
T3145 6990-6992 , denotes ,
T3146 6992-6996 CD denotes 29.3
T3147 6996-6998 , denotes ,
T3148 6998-7002 CD denotes 29.5
T3149 7002-7004 , denotes ,
T3150 7004-7008 CD denotes 29.7
T3151 7008-7010 , denotes ,
T3152 7010-7012 CD denotes 30
T3153 7013-7016 CC denotes and
T3154 7017-7019 CD denotes 31
T3155 7019-7020 -RRB- denotes )
T3156 7020-7021 . denotes .
T3157 7021-7102 sentence denotes These novel exons were flanked by well-defined introns that obey the GT-AG rule.
T3158 7022-7027 DT denotes These
T3160 7028-7033 JJ denotes novel
T3159 7034-7039 NNS denotes exons
T3162 7040-7044 VBD denotes were
T3161 7045-7052 VBN denotes flanked
T3163 7053-7055 IN denotes by
T3164 7056-7060 RB denotes well
T3166 7060-7061 HYPH denotes -
T3165 7061-7068 VBN denotes defined
T3167 7069-7076 NNS denotes introns
T3168 7077-7081 WDT denotes that
T3169 7082-7086 VBP denotes obey
T3170 7087-7090 DT denotes the
T3172 7091-7093 NN denotes GT
T3174 7093-7094 HYPH denotes -
T3173 7094-7096 NN denotes AG
T3171 7097-7101 NN denotes rule
T3175 7101-7102 . denotes .
T3176 7102-7156 sentence denotes Exon organization of each clone was shown in Fig. 8C.
T3177 7103-7107 NN denotes Exon
T3178 7108-7120 NN denotes organization
T3180 7121-7123 IN denotes of
T3181 7124-7128 DT denotes each
T3182 7129-7134 NN denotes clone
T3183 7135-7138 VBD denotes was
T3179 7139-7144 VBN denotes shown
T3184 7145-7147 IN denotes in
T3185 7148-7152 NN denotes Fig.
T3186 7153-7155 NN denotes 8C
T3187 7155-7156 . denotes .
T3188 7156-7252 sentence denotes Characteristic feature of ADAM22 transcripts is tissue specific insertion or skipping of exons.
T3189 7157-7171 JJ denotes Characteristic
T3190 7172-7179 NN denotes feature
T3192 7180-7182 IN denotes of
T3193 7183-7189 NN denotes ADAM22
T3194 7190-7201 NNS denotes transcripts
T3191 7202-7204 VBZ denotes is
T3195 7205-7211 NN denotes tissue
T3196 7212-7220 JJ denotes specific
T3197 7221-7230 NN denotes insertion
T3198 7231-7233 CC denotes or
T3199 7234-7242 NN denotes skipping
T3200 7243-7245 IN denotes of
T3201 7246-7251 NNS denotes exons
T3202 7251-7252 . denotes .
T3203 7252-7431 sentence denotes Because the number of nucleotides of exons 26, 27, 27L, 29.3, 29.5 and 29.7 is a multiple of 3, insertion or skipping of these exons will not change the downstream reading frame.
T3204 7253-7260 IN denotes Because
T3206 7261-7264 DT denotes the
T3207 7265-7271 NN denotes number
T3208 7272-7274 IN denotes of
T3209 7275-7286 NNS denotes nucleotides
T3210 7287-7289 IN denotes of
T3211 7290-7295 NNS denotes exons
T3212 7296-7298 CD denotes 26
T3213 7298-7300 , denotes ,
T3214 7300-7302 CD denotes 27
T3215 7302-7304 , denotes ,
T3216 7304-7307 CD denotes 27L
T3217 7307-7309 , denotes ,
T3218 7309-7313 CD denotes 29.3
T3219 7313-7315 , denotes ,
T3220 7315-7319 CD denotes 29.5
T3221 7320-7323 CC denotes and
T3222 7324-7328 CD denotes 29.7
T3205 7329-7331 VBZ denotes is
T3224 7332-7333 DT denotes a
T3225 7334-7342 NN denotes multiple
T3226 7343-7345 IN denotes of
T3227 7346-7347 CD denotes 3
T3228 7347-7349 , denotes ,
T3229 7349-7358 NN denotes insertion
T3230 7359-7361 CC denotes or
T3231 7362-7370 NN denotes skipping
T3232 7371-7373 IN denotes of
T3233 7374-7379 DT denotes these
T3234 7380-7385 NNS denotes exons
T3235 7386-7390 MD denotes will
T3236 7391-7394 RB denotes not
T3223 7395-7401 VB denotes change
T3237 7402-7405 DT denotes the
T3239 7406-7416 JJ denotes downstream
T3240 7417-7424 NN denotes reading
T3238 7425-7430 NN denotes frame
T3241 7430-7431 . denotes .
T3242 7431-7501 sentence denotes Known peptide motives were not found in the newly isolated sequences.
T3243 7432-7437 JJ denotes Known
T3245 7438-7445 NN denotes peptide
T3244 7446-7453 NNS denotes motives
T3247 7454-7458 VBD denotes were
T3248 7459-7462 RB denotes not
T3246 7463-7468 VBN denotes found
T3249 7469-7471 IN denotes in
T3250 7472-7475 DT denotes the
T3252 7476-7481 RB denotes newly
T3253 7482-7490 VBN denotes isolated
T3251 7491-7500 NNS denotes sequences
T3254 7500-7501 . denotes .
T3255 7501-7715 sentence denotes This RT-PCR analysis is summarized as follows: ADAM22 transcripts were roughly subdivided in 3 groups, CNS-form (containing exon 27), DRG-form (containing exon 27L) and Schwann-cell-form (skipping exon 26 and 27).
T3256 7502-7506 DT denotes This
T3258 7507-7509 NN denotes RT
T3260 7509-7510 HYPH denotes -
T3259 7510-7513 NN denotes PCR
T3257 7514-7522 NN denotes analysis
T3262 7523-7525 VBZ denotes is
T3261 7526-7536 VBN denotes summarized
T3264 7537-7539 IN denotes as
T3265 7540-7547 VBZ denotes follows
T3266 7547-7549 : denotes :
T3267 7549-7555 NN denotes ADAM22
T3268 7556-7567 NNS denotes transcripts
T3269 7568-7572 VBD denotes were
T3270 7573-7580 RB denotes roughly
T3263 7581-7591 VBN denotes subdivided
T3271 7592-7594 IN denotes in
T3272 7595-7596 CD denotes 3
T3273 7597-7603 NNS denotes groups
T3274 7603-7605 , denotes ,
T3275 7605-7608 NN denotes CNS
T3277 7608-7609 HYPH denotes -
T3276 7609-7613 NN denotes form
T3278 7614-7615 -LRB- denotes (
T3279 7615-7625 VBG denotes containing
T3280 7626-7630 NN denotes exon
T3281 7631-7633 CD denotes 27
T3282 7633-7634 -RRB- denotes )
T3283 7634-7636 , denotes ,
T3284 7636-7639 NN denotes DRG
T3286 7639-7640 HYPH denotes -
T3285 7640-7644 NN denotes form
T3287 7645-7646 -LRB- denotes (
T3288 7646-7656 VBG denotes containing
T3289 7657-7661 NN denotes exon
T3290 7662-7665 NN denotes 27L
T3291 7665-7666 -RRB- denotes )
T3292 7667-7670 CC denotes and
T3293 7671-7678 NNP denotes Schwann
T3295 7678-7679 HYPH denotes -
T3294 7679-7683 NN denotes cell
T3297 7683-7684 HYPH denotes -
T3296 7684-7688 NN denotes form
T3298 7689-7690 -LRB- denotes (
T3299 7690-7698 VBG denotes skipping
T3300 7699-7703 NN denotes exon
T3301 7704-7706 CD denotes 26
T3302 7707-7710 CC denotes and
T3303 7711-7713 CD denotes 27
T3304 7713-7714 -RRB- denotes )
T3305 7714-7715 . denotes .
T3306 7715-7863 sentence denotes From these results, we concluded that ADAM22 is expressed in a cell-type specific manner, and plays an essential role in myelinogenesis in the PNS.
T3307 7716-7720 IN denotes From
T3309 7721-7726 DT denotes these
T3310 7727-7734 NNS denotes results
T3311 7734-7736 , denotes ,
T3312 7736-7738 PRP denotes we
T3308 7739-7748 VBD denotes concluded
T3313 7749-7753 IN denotes that
T3315 7754-7760 NN denotes ADAM22
T3316 7761-7763 VBZ denotes is
T3314 7764-7773 VBN denotes expressed
T3317 7774-7776 IN denotes in
T3318 7777-7778 DT denotes a
T3320 7779-7783 NN denotes cell
T3322 7783-7784 HYPH denotes -
T3321 7784-7788 NN denotes type
T3323 7789-7797 JJ denotes specific
T3319 7798-7804 NN denotes manner
T3324 7804-7806 , denotes ,
T3325 7806-7809 CC denotes and
T3326 7810-7815 VBZ denotes plays
T3327 7816-7818 DT denotes an
T3329 7819-7828 JJ denotes essential
T3328 7829-7833 NN denotes role
T3330 7834-7836 IN denotes in
T3331 7837-7851 NN denotes myelinogenesis
T3332 7852-7854 IN denotes in
T3333 7855-7858 DT denotes the
T3334 7859-7862 NN denotes PNS
T3335 7862-7863 . denotes .
R1000 T1605 T1584 parataxis Table,was
R1001 T1606 T1605 nummod 2,Table
R1002 T1607 T1605 punct ),Table
R1003 T1608 T1584 punct .,was
R1004 T1610 T1611 amod Homozygous,mutants
R1005 T1611 T1612 nsubj mutants,died
R1006 T1613 T1612 npadvmod one,died
R1007 T1614 T1613 prep by,one
R1008 T1615 T1614 pobj one,by
R1009 T1616 T1612 prep after,died
R1010 T1617 T1616 pobj P10,after
R1011 T1618 T1612 punct ", ",died
R1012 T1619 T1612 cc and,died
R1013 T1620 T1621 det all,homozygotes
R1014 T1621 T1622 nsubj homozygotes,died
R1015 T1622 T1612 conj died,died
R1016 T1623 T1622 prep before,died
R1017 T1624 T1623 pobj P20,before
R1018 T1625 T1622 punct .,died
R1019 T1627 T1628 prep Of,survived
R1020 T1629 T1630 amod more,100
R1021 T1630 T1632 nummod 100,homozygotes
R1022 T1631 T1630 quantmod than,100
R1023 T1632 T1627 pobj homozygotes,Of
R1024 T1633 T1634 nsubj we,produced
R1025 T1634 T1632 advcl produced,homozygotes
R1026 T1635 T1634 aux have,produced
R1027 T1636 T1628 punct ", ",survived
R1028 T1637 T1628 nsubj none,survived
R1029 T1638 T1628 aux have,survived
R1030 T1639 T1640 amod more,25
R1031 T1640 T1642 nummod 25,days
R1032 T1641 T1640 quantmod than,25
R1033 T1642 T1628 npadvmod days,survived
R1034 T1643 T1642 prep after,days
R1035 T1644 T1643 pobj birth,after
R1036 T1645 T1628 punct .,survived
R1037 T1647 T1648 advmod Meanwhile,looked
R1038 T1649 T1648 punct ", ",looked
R1039 T1650 T1651 amod heterozygous,mutants
R1040 T1651 T1648 nsubj mutants,looked
R1041 T1652 T1648 acomp normal,looked
R1042 T1653 T1648 punct ", ",looked
R1043 T1654 T1648 conj were,looked
R1044 T1655 T1654 acomp fertile,were
R1045 T1656 T1654 punct ", ",were
R1046 T1657 T1654 cc and,were
R1047 T1658 T1654 conj survived,were
R1048 T1659 T1658 prep for,survived
R1049 T1660 T1661 amod more,one
R1050 T1661 T1663 nummod one,year
R1051 T1662 T1661 quantmod than,one
R1052 T1663 T1659 pobj year,for
R1053 T1664 T1658 prep without,survived
R1054 T1665 T1666 amod obvious,defects
R1055 T1666 T1664 pobj defects,without
R1056 T1667 T1648 punct .,looked
R1059 T1961 T1960 prep of,Histopathology
R1060 T1962 T1963 npadvmod ADAM22,deficient
R1061 T1963 T1965 amod deficient,mice
R1062 T1964 T1963 punct -,deficient
R1063 T1965 T1961 pobj mice,of
R1064 T1967 T1968 nsubj We,reported
R1065 T1969 T1968 aux have,reported
R1066 T1970 T1971 det the,expression
R1067 T1971 T1968 dobj expression,reported
R1068 T1972 T1971 amod predominant,expression
R1069 T1973 T1971 prep of,expression
R1070 T1974 T1975 compound ADAM22,mRNA
R1071 T1975 T1973 pobj mRNA,of
R1072 T1976 T1971 prep in,expression
R1073 T1977 T1978 det the,brain
R1074 T1978 T1976 pobj brain,in
R1075 T1979 T1980 amod human,mouse
R1076 T1980 T1978 compound mouse,brain
R1077 T1981 T1980 cc and,mouse
R1078 T1982 T1968 prep by,reported
R1079 T1983 T1984 compound Northern,blot
R1080 T1984 T1985 compound blot,analysis
R1081 T1985 T1982 pobj analysis,by
R1082 T1986 T1987 punct [,14
R1083 T1987 T1968 parataxis 14,reported
R1084 T1988 T1987 nummod 13,14
R1085 T1989 T1987 punct ",",14
R1086 T1990 T1987 punct ],14
R1087 T1991 T1968 punct .,reported
R1088 T1993 T1994 aux To,determine
R1089 T1994 T1995 advcl determine,performed
R1090 T1996 T1997 det the,distribution
R1091 T1997 T1994 dobj distribution,determine
R1092 T1998 T1997 prep of,distribution
R1093 T1999 T2000 compound ADAM22,transcript
R1094 T2000 T1998 pobj transcript,of
R1095 T2001 T1994 prep in,determine
R1096 T2002 T2003 det the,CNS
R1097 T2003 T2001 pobj CNS,in
R1098 T2004 T2003 amod adult,CNS
R1099 T2005 T2003 compound mouse,CNS
R1100 T2006 T1994 advmod precisely,determine
R1101 T2007 T1995 punct ", ",performed
R1102 T2008 T2009 advmod in,situ
R1103 T2009 T2010 amod situ,analysis
R1104 T2010 T1995 nsubjpass analysis,performed
R1105 T2011 T2010 compound hybridisation,analysis
R1106 T2012 T1995 auxpass was,performed
R1107 T2013 T1995 advcl using,performed
R1108 T2014 T2015 advmod 35S,labeled
R1109 T2015 T2017 amod labeled,probe
R1110 T2016 T2015 punct -,labeled
R1111 T2017 T2013 dobj probe,using
R1112 T2018 T2017 compound RNA,probe
R1113 T2019 T1995 punct .,performed
R1114 T2021 T2022 mark As,shown
R1115 T2022 T2023 advcl shown,expressed
R1116 T2024 T2022 prep in,shown
R1117 T2025 T2024 pobj Fig.,in
R1118 T2026 T2025 nummod 3,Fig.
R1119 T2027 T2023 punct ", ",expressed
R1120 T2028 T2029 compound ADAM22,mRNA
R1121 T2029 T2023 nsubjpass mRNA,expressed
R1122 T2030 T2023 auxpass was,expressed
R1123 T2031 T2023 prep throughout,expressed
R1124 T2032 T2033 det the,CNS
R1125 T2033 T2031 pobj CNS,throughout
R1126 T2034 T2033 amod adult,CNS
R1127 T2035 T2033 compound mouse,CNS
R1128 T2036 T2023 punct .,expressed
R1129 T2038 T2039 amod Strong,signal
R1130 T2039 T2040 nsubjpass signal,detected
R1131 T2041 T2040 auxpass was,detected
R1132 T2042 T2040 prep in,detected
R1133 T2043 T2044 det the,cells
R1134 T2044 T2042 pobj cells,in
R1135 T2045 T2044 amod cerebellar,cells
R1136 T2046 T2044 compound granule,cells
R1137 T2047 T2044 cc and,cells
R1138 T2048 T2049 amod hippocampal,formation
R1139 T2049 T2044 conj formation,cells
R1140 T2050 T2040 punct .,detected
R1141 T2052 T2053 prep In,restricted
R1142 T2054 T2055 det the,cord
R1143 T2055 T2052 pobj cord,In
R1144 T2056 T2055 amod spinal,cord
R1145 T2057 T2053 punct ", ",restricted
R1146 T2058 T2059 compound hybridisation,signal
R1147 T2059 T2053 nsubjpass signal,restricted
R1148 T2060 T2053 auxpass was,restricted
R1149 T2061 T2053 prep to,restricted
R1150 T2062 T2063 det the,matter
R1151 T2063 T2061 pobj matter,to
R1152 T2064 T2063 amod grey,matter
R1153 T2065 T2053 punct .,restricted
R1154 T2067 T2068 det The,distribution
R1155 T2068 T2069 nsubj distribution,was
R1156 T2070 T2068 prep of,distribution
R1157 T2071 T2072 compound ADAM22,transcripts
R1158 T2072 T2070 pobj transcripts,of
R1159 T2073 T2068 prep in,distribution
R1160 T2074 T2075 det the,CNS
R1161 T2075 T2073 pobj CNS,in
R1162 T2076 T2077 advmod quite,similar
R1163 T2077 T2069 acomp similar,was
R1164 T2078 T2077 prep with,similar
R1165 T2079 T2078 pobj that,with
R1166 T2080 T2079 prep of,that
R1167 T2081 T2080 pobj ADAM11,of
R1168 T2082 T2081 punct ", ",ADAM11
R1169 T2083 T2084 poss whose,expression
R1170 T2084 T2086 dep expression,reported
R1171 T2085 T2084 amod neuronal,expression
R1172 T2086 T2081 relcl reported,ADAM11
R1173 T2087 T2086 aux has,reported
R1174 T2088 T2086 auxpass been,reported
R1175 T2089 T2090 punct [,19
R1176 T2090 T2069 parataxis 19,was
R1177 T2091 T2090 punct ],19
R1178 T2092 T2069 punct .,was
R1179 T2094 T2095 det These,results
R1180 T2095 T2096 nsubj results,suggest
R1181 T2097 T2098 mark that,is
R1182 T2098 T2096 ccomp is,suggest
R1183 T2099 T2100 det the,expression
R1184 T2100 T2098 nsubj expression,is
R1185 T2101 T2100 compound ADAM22,expression
R1186 T2102 T2100 compound mRNA,expression
R1187 T2103 T2098 acomp neuronal,is
R1188 T2104 T2098 prep in,is
R1189 T2105 T2106 det the,CNS
R1190 T2106 T2104 pobj CNS,in
R1191 T2107 T2106 compound mouse,CNS
R1192 T2108 T2096 punct .,suggest
R1193 T2110 T2111 prep Based,performed
R1194 T2112 T2110 prep on,Based
R1195 T2113 T2114 det the,pattern
R1196 T2114 T2112 pobj pattern,on
R1197 T2115 T2114 compound mRNA,pattern
R1198 T2116 T2114 compound distribution,pattern
R1199 T2117 T2111 punct ", ",performed
R1200 T2118 T2111 nsubj we,performed
R1201 T2119 T2120 amod immunohistopathological,analysis
R1202 T2120 T2111 dobj analysis,performed
R1203 T2121 T2120 prep of,analysis
R1204 T2122 T2123 det the,cerebellum
R1205 T2123 T2121 pobj cerebellum,of
R1206 T2124 T2123 cc and,cerebellum
R1207 T2125 T2123 conj hippocampus,cerebellum
R1208 T2126 T2111 advmod extensively,performed
R1209 T2127 T2111 punct .,performed
R1210 T2129 T2130 prep Despite,formed
R1211 T2131 T2132 det the,level
R1212 T2132 T2129 pobj level,Despite
R1213 T2133 T2132 amod high,level
R1214 T2134 T2132 prep of,level
R1215 T2135 T2134 pobj expression,of
R1216 T2136 T2135 prep of,expression
R1217 T2137 T2138 compound ADAM22,mRNAs
R1218 T2138 T2136 pobj mRNAs,of
R1219 T2139 T2132 prep in,level
R1220 T2140 T2141 det the,cells
R1221 T2141 T2139 pobj cells,in
R1222 T2142 T2141 amod cerebellar,cells
R1223 T2143 T2141 compound granule,cells
R1224 T2144 T2130 punct ", ",formed
R1225 T2145 T2146 compound granule,layer
R1226 T2146 T2130 nsubjpass layer,formed
R1227 T2147 T2146 compound cell,layer
R1228 T2148 T2130 auxpass was,formed
R1229 T2149 T2130 advmod normally,formed
R1230 T2150 T2130 cc and,formed
R1231 T2151 T2152 compound Purkinje,cell
R1232 T2152 T2153 compound cell,morphology
R1233 T2153 T2154 nsubj morphology,looked
R1234 T2154 T2130 conj looked,formed
R1235 T2155 T2156 punct (,staining
R1236 T2156 T2153 parataxis staining,morphology
R1237 T2157 T2156 npadvmod calbindin,staining
R1238 T2158 T2156 punct -,staining
R1239 T2159 T2156 punct ),staining
R1240 T2160 T2153 prep of,morphology
R1241 T2161 T2162 det the,mouse
R1242 T2162 T2160 pobj mouse,of
R1243 T2163 T2162 compound mutant,mouse
R1244 T2164 T2154 acomp intact,looked
R1245 T2165 T2166 punct (,4A
R1246 T2166 T2154 parataxis 4A,looked
R1247 T2167 T2166 compound Figs.,4A
R1248 T2168 T2169 punct –,D
R1249 T2169 T2166 prep D,4A
R1250 T2170 T2166 punct ),4A
R1251 T2171 T2130 punct .,formed
R1252 T2173 T2174 amod Hippocampal,formation
R1253 T2174 T2175 nsubjpass formation,formed
R1254 T2176 T2175 auxpass was,formed
R1255 T2177 T2175 advmod also,formed
R1256 T2178 T2175 advmod normally,formed
R1257 T2179 T2180 punct (,4I
R1258 T2180 T2175 parataxis 4I,formed
R1259 T2181 T2180 compound Figs.,4I
R1260 T2182 T2183 punct –,J
R1261 T2183 T2180 prep J,4I
R1262 T2184 T2180 punct ),4I
R1263 T2185 T2175 punct .,formed
R1264 T2187 T2188 det These,results
R1265 T2188 T2189 nsubj results,suggest
R1266 T2190 T2191 mark that,impaired
R1267 T2191 T2189 ccomp impaired,suggest
R1268 T2192 T2193 amod neuronal,cell
R1269 T2193 T2194 compound cell,migration
R1270 T2194 T2191 nsubjpass migration,impaired
R1271 T2195 T2191 auxpass was,impaired
R1272 T2196 T2191 neg not,impaired
R1273 T2197 T2191 prep in,impaired
R1274 T2198 T2199 det the,mouse
R1275 T2199 T2197 pobj mouse,in
R1276 T2200 T2199 compound mutant,mouse
R1277 T2201 T2189 punct .,suggest
R1278 T2203 T2204 compound Myelin,formation
R1279 T2204 T2206 nsubj formation,was
R1280 T2205 T2204 punct -,formation
R1281 T2207 T2204 acl detected,formation
R1282 T2208 T2207 prep by,detected
R1283 T2209 T2210 nmod MBP,staining
R1284 T2210 T2208 pobj staining,by
R1285 T2211 T2209 punct (,MBP
R1286 T2212 T2213 nmod myelin,protein
R1287 T2213 T2209 appos protein,MBP
R1288 T2214 T2213 amod basic,protein
R1289 T2215 T2210 punct ),staining
R1290 T2216 T2210 prep of,staining
R1291 T2217 T2218 det the,mutant
R1292 T2218 T2216 pobj mutant,of
R1293 T2219 T2204 prep in,formation
R1294 T2220 T2221 det the,cerebellum
R1295 T2221 T2219 pobj cerebellum,in
R1296 T2222 T2223 punct (,4H
R1297 T2223 T2221 parataxis 4H,cerebellum
R1298 T2224 T2223 compound Fig.,4H
R1299 T2225 T2223 punct ),4H
R1300 T2226 T2221 cc and,cerebellum
R1301 T2227 T2228 amod spinal,cord
R1302 T2228 T2221 conj cord,cerebellum
R1303 T2229 T2230 punct (,4L
R1304 T2230 T2228 parataxis 4L,cord
R1305 T2231 T2230 compound Fig.,4L
R1306 T2232 T2230 punct ),4L
R1307 T2233 T2206 advmod also,was
R1308 T2234 T2206 acomp indistinguishable,was
R1309 T2235 T2234 prep with,indistinguishable
R1310 T2236 T2237 det the,littermate
R1311 T2237 T2235 pobj littermate,with
R1312 T2238 T2239 amod wild,type
R1313 T2239 T2237 compound type,littermate
R1314 T2240 T2239 punct -,type
R1315 T2241 T2242 punct (,K
R1316 T2242 T2206 parataxis K,was
R1317 T2243 T2242 nmod Figs.,K
R1318 T2244 T2242 nmod 4G,K
R1319 T2245 T2242 punct ",",K
R1320 T2246 T2242 punct ),K
R1321 T2247 T2206 punct .,was
R1322 T2249 T2250 prep In,find
R1323 T2251 T2249 pobj summary,In
R1324 T2252 T2250 punct ", ",find
R1325 T2253 T2250 nsubj we,find
R1326 T2254 T2250 aux could,find
R1327 T2255 T2250 neg not,find
R1328 T2256 T2257 det any,signs
R1329 T2257 T2250 dobj signs,find
R1330 T2258 T2257 prep of,signs
R1331 T2259 T2258 pobj abnormalities,of
R1332 T2260 T2250 prep in,find
R1333 T2261 T2262 det the,brain
R1334 T2262 T2260 pobj brain,in
R1335 T2263 T2262 compound mutant,brain
R1336 T2264 T2262 compound mouse,brain
R1337 T2265 T2250 prep by,find
R1338 T2266 T2267 det the,examination
R1339 T2267 T2265 pobj examination,by
R1340 T2268 T2269 npadvmod light,microscopic
R1341 T2269 T2267 amod microscopic,examination
R1342 T2270 T2250 punct .,find
R1343 T2272 T2273 advmod Next,analysed
R1344 T2274 T2273 punct ", ",analysed
R1345 T2275 T2276 det the,cord
R1346 T2276 T2273 nsubjpass cord,analysed
R1347 T2277 T2276 amod spinal,cord
R1348 T2278 T2276 cc and,cord
R1349 T2279 T2280 amod peripheral,nerves
R1350 T2280 T2276 conj nerves,cord
R1351 T2281 T2276 prep of,cord
R1352 T2282 T2283 det each,genotype
R1353 T2283 T2281 pobj genotype,of
R1354 T2284 T2273 auxpass were,analysed
R1355 T2285 T2273 prep by,analysed
R1356 T2286 T2287 nmod toluidine,stain
R1357 T2287 T2285 pobj stain,by
R1358 T2288 T2287 amod blue,stain
R1359 T2289 T2287 punct ", ",stain
R1360 T2290 T2291 dep which,reveals
R1361 T2291 T2287 relcl reveals,stain
R1362 T2292 T2293 compound myelin,formation
R1363 T2293 T2291 dobj formation,reveals
R1364 T2294 T2273 punct .,analysed
R1365 T2296 T2297 advmod Surprisingly,observed
R1366 T2298 T2297 punct ", ",observed
R1367 T2299 T2297 nsubjpass lack,observed
R1368 T2300 T2299 prep of,lack
R1369 T2301 T2300 pobj myelin,of
R1370 T2302 T2301 cc or,myelin
R1371 T2303 T2304 amod thin,myelin
R1372 T2304 T2301 conj myelin,myelin
R1373 T2305 T2297 auxpass was,observed
R1374 T2306 T2297 prep in,observed
R1375 T2307 T2308 det the,nerves
R1376 T2308 T2306 pobj nerves,in
R1377 T2309 T2308 amod sciatic,nerves
R1378 T2310 T2311 punct (,5B
R1379 T2311 T2308 parataxis 5B,nerves
R1380 T2312 T2311 compound Fig.,5B
R1381 T2313 T2311 punct ),5B
R1382 T2314 T2308 cc and,nerves
R1383 T2315 T2316 amod trigeminal,nerves
R1384 T2316 T2308 conj nerves,nerves
R1385 T2317 T2318 punct (,5D
R1386 T2318 T2316 parataxis 5D,nerves
R1387 T2319 T2318 compound Fig.,5D
R1388 T2320 T2318 punct ),5D
R1389 T2321 T2297 prep in,observed
R1390 T2322 T2323 amod homozygous,mutants
R1391 T2323 T2321 pobj mutants,in
R1392 T2324 T2297 punct .,observed
R1393 T2326 T2327 mark Because,observed
R1394 T2327 T2331 advcl observed,suggested
R1395 T2328 T2329 det no,lesions
R1396 T2329 T2327 nsubjpass lesions,observed
R1397 T2330 T2327 auxpass were,observed
R1398 T2332 T2327 prep in,observed
R1399 T2333 T2334 det the,cord
R1400 T2334 T2332 pobj cord,in
R1401 T2335 T2334 amod spinal,cord
R1402 T2336 T2337 punct (,5F
R1403 T2337 T2327 parataxis 5F,observed
R1404 T2338 T2337 compound Fig.,5F
R1405 T2339 T2337 punct ),5F
R1406 T2340 T2331 punct ", ",suggested
R1407 T2341 T2331 nsubjpass it,suggested
R1408 T2342 T2331 auxpass was,suggested
R1409 T2343 T2344 mark that,occurred
R1410 T2344 T2331 ccomp occurred,suggested
R1411 T2345 T2346 compound Schwann,cell
R1412 T2346 T2347 npadvmod cell,specific
R1413 T2347 T2348 amod specific,defect
R1414 T2348 T2344 nsubj defect,occurred
R1415 T2349 T2348 compound myelination,defect
R1416 T2350 T2344 prep in,occurred
R1417 T2351 T2352 det the,mice
R1418 T2352 T2350 pobj mice,in
R1419 T2353 T2352 amod homozygous,mice
R1420 T2354 T2331 punct .,suggested
R1421 T2356 T2357 aux To,analyse
R1422 T2357 T2358 advcl analyse,performed
R1423 T2359 T2360 det the,state
R1424 T2360 T2357 dobj state,analyse
R1425 T2361 T2360 prep of,state
R1426 T2362 T2363 amod myelinating,cells
R1427 T2363 T2361 pobj cells,of
R1428 T2364 T2363 compound Schwann,cells
R1429 T2365 T2363 cc and,cells
R1430 T2366 T2363 conj axons,cells
R1431 T2367 T2358 punct ", ",performed
R1432 T2368 T2369 npadvmod electron,microscopic
R1433 T2369 T2370 amod microscopic,analysis
R1434 T2370 T2358 nsubjpass analysis,performed
R1435 T2371 T2372 punct (,EM
R1436 T2372 T2369 parataxis EM,microscopic
R1437 T2373 T2372 punct ),EM
R1438 T2374 T2370 prep of,analysis
R1439 T2375 T2376 det the,nerve
R1440 T2376 T2374 pobj nerve,of
R1441 T2377 T2376 amod sciatic,nerve
R1442 T2378 T2358 auxpass was,performed
R1443 T2379 T2380 punct (,Fig.
R1444 T2380 T2358 parataxis Fig.,performed
R1445 T2381 T2380 nummod 6,Fig.
R1446 T2382 T2380 punct ),Fig.
R1447 T2383 T2358 punct .,performed
R1448 T2385 T2386 compound Schwann,cells
R1449 T2386 T2387 nsubj cells,formed
R1450 T2388 T2387 dobj thin,formed
R1451 T2389 T2388 cc or,thin
R1452 T2390 T2391 nmod no,myelin
R1453 T2391 T2388 conj myelin,thin
R1454 T2392 T2387 prep in,formed
R1455 T2393 T2394 det the,mutant
R1456 T2394 T2392 pobj mutant,in
R1457 T2395 T2394 amod homozygous,mutant
R1458 T2396 T2397 punct (,6B
R1459 T2397 T2387 parataxis 6B,formed
R1460 T2398 T2397 compound Fig.,6B
R1461 T2399 T2397 punct ),6B
R1462 T2400 T2387 punct .,formed
R1463 T2402 T2403 prep In,showed
R1464 T2404 T2402 pobj contrast,In
R1465 T2405 T2403 punct ", ",showed
R1466 T2406 T2407 amod heterozygous,mice
R1467 T2407 T2403 nsubj mice,showed
R1468 T2408 T2409 amod complete,myelination
R1469 T2409 T2403 dobj myelination,showed
R1470 T2410 T2411 punct (,6A
R1471 T2411 T2403 parataxis 6A,showed
R1472 T2412 T2411 compound Fig.,6A
R1473 T2413 T2411 punct ),6A
R1474 T2414 T2403 punct .,showed
R1475 T2416 T2417 nsubj Morphology,looked
R1476 T2418 T2416 prep of,Morphology
R1477 T2419 T2420 det the,axons
R1478 T2420 T2418 pobj axons,of
R1479 T2421 T2417 acomp normal,looked
R1480 T2422 T2417 prep in,looked
R1481 T2423 T2424 det each,genotype
R1482 T2424 T2422 pobj genotype,in
R1483 T2425 T2417 punct .,looked
R1484 T2427 T2428 amod Immunohistochemical,analysis
R1485 T2428 T2429 nsubj analysis,showed
R1486 T2430 T2428 prep of,analysis
R1487 T2431 T2432 det the,nerve
R1488 T2432 T2430 pobj nerve,of
R1489 T2433 T2432 amod sciatic,nerve
R1490 T2434 T2435 mark that,were
R1491 T2435 T2429 ccomp were,showed
R1492 T2436 T2437 amod increased,number
R1493 T2437 T2435 nsubj number,were
R1494 T2438 T2437 prep of,number
R1495 T2439 T2438 pobj nuclei,of
R1496 T2440 T2441 punct (,7B
R1497 T2441 T2437 parataxis 7B,number
R1498 T2442 T2441 compound Fig.,7B
R1499 T2443 T2441 punct ),7B
R1500 T2444 T2437 cc and,number
R1501 T2445 T2446 amod reduced,staining
R1502 T2446 T2437 conj staining,number
R1503 T2447 T2446 prep of,staining
R1504 T2448 T2447 pobj MBP,of
R1505 T2449 T2450 punct (,7D
R1506 T2450 T2446 parataxis 7D,staining
R1507 T2451 T2450 compound Fig.,7D
R1508 T2452 T2450 punct ),7D
R1509 T2453 T2435 acomp remarkable,were
R1510 T2454 T2435 prep in,were
R1511 T2455 T2456 det the,mutant
R1512 T2456 T2454 pobj mutant,in
R1513 T2457 T2456 amod homozygous,mutant
R1514 T2458 T2429 punct .,showed
R1515 T2460 T2461 det The,marker
R1516 T2461 T2464 nsubjpass marker,observed
R1517 T2462 T2463 compound Schwann,cell
R1518 T2463 T2461 compound cell,marker
R1519 T2465 T2461 punct ", ",marker
R1520 T2466 T2467 compound S100,signal
R1521 T2467 T2461 appos signal,marker
R1522 T2468 T2467 compound staining,signal
R1523 T2469 T2464 auxpass was,observed
R1524 T2470 T2464 advmod intensely,observed
R1525 T2471 T2464 prep in,observed
R1526 T2472 T2473 det the,homozygote
R1527 T2473 T2471 pobj homozygote,in
R1528 T2474 T2475 advmod as,as
R1529 T2475 T2473 cc as,homozygote
R1530 T2476 T2475 advmod well,as
R1531 T2477 T2478 amod wild,type
R1532 T2478 T2473 conj type,homozygote
R1533 T2479 T2478 punct -,type
R1534 T2480 T2481 punct (,F
R1535 T2481 T2464 parataxis F,observed
R1536 T2482 T2481 nmod Figs.,F
R1537 T2483 T2481 nmod 7E,F
R1538 T2484 T2481 punct ", ",F
R1539 T2485 T2481 punct ),F
R1540 T2486 T2464 punct .,observed
R1541 T2488 T2489 det These,results
R1542 T2489 T2490 nsubj results,suggest
R1543 T2491 T2492 mark that,was
R1544 T2492 T2490 ccomp was,suggest
R1545 T2493 T2492 nsubj proliferation,was
R1546 T2494 T2493 prep of,proliferation
R1547 T2495 T2496 det the,cells
R1548 T2496 T2494 pobj cells,of
R1549 T2497 T2496 compound Schwann,cells
R1550 T2498 T2492 neg not,was
R1551 T2499 T2492 acomp impaired,was
R1552 T2500 T2492 cc but,was
R1553 T2501 T2502 nsubj differentiation,is
R1554 T2502 T2492 conj is,was
R1555 T2503 T2504 advmod severely,delayed
R1556 T2504 T2502 acomp delayed,is
R1557 T2505 T2502 prep in,is
R1558 T2506 T2507 det the,mice
R1559 T2507 T2505 pobj mice,in
R1560 T2508 T2509 npadvmod ADAM22,deficient
R1561 T2509 T2507 amod deficient,mice
R1562 T2510 T2509 punct -,deficient
R1563 T2511 T2490 punct .,suggest
R1568 T2746 T2747 amod Novel,variants
R1569 T2747 T2750 nsubj variants,isolated
R1570 T2748 T2747 compound ADAM22,variants
R1571 T2749 T2747 compound transcript,variants
R1572 T2751 T2750 prep from,isolated
R1573 T2752 T2753 det the,system
R1574 T2753 T2751 pobj system,from
R1575 T2754 T2753 amod peripheral,system
R1576 T2755 T2753 amod nervous,system
R1577 T2757 T2758 prep In,reported
R1578 T2759 T2760 det the,case
R1579 T2760 T2757 pobj case,In
R1580 T2761 T2760 prep of,case
R1581 T2762 T2763 amod human,ADAM22
R1582 T2763 T2761 pobj ADAM22,of
R1583 T2764 T2758 punct ", ",reported
R1584 T2765 T2758 nsubjpass isolation,reported
R1585 T2766 T2765 prep of,isolation
R1586 T2767 T2768 nummod five,variants
R1587 T2768 T2766 pobj variants,of
R1588 T2769 T2768 compound splicing,variants
R1589 T2770 T2765 cc and,isolation
R1590 T2771 T2765 conj existence,isolation
R1591 T2772 T2771 prep of,existence
R1592 T2773 T2774 nummod two,exons
R1593 T2774 T2772 pobj exons,of
R1594 T2775 T2774 amod terminating,exons
R1595 T2776 T2758 aux have,reported
R1596 T2777 T2758 auxpass been,reported
R1597 T2778 T2779 punct [,21
R1598 T2779 T2758 parataxis 21,reported
R1599 T2780 T2779 nummod 13,21
R1600 T2781 T2779 punct ",",21
R1601 T2782 T2779 nummod 20,21
R1602 T2783 T2779 punct ",",21
R1603 T2784 T2779 punct ],21
R1604 T2785 T2758 punct .,reported
R1605 T2787 T2788 advmod However,identified
R1606 T2789 T2788 punct ", ",identified
R1607 T2790 T2791 det the,exon
R1608 T2791 T2788 nsubjpass exon,identified
R1609 T2792 T2791 amod latter,exon
R1610 T2793 T2791 amod terminating,exon
R1611 T2794 T2791 punct (,exon
R1612 T2795 T2791 appos exon,exon
R1613 T2796 T2795 nummod 31,exon
R1614 T2797 T2791 punct ),exon
R1615 T2798 T2791 prep of,exon
R1616 T2799 T2800 det the,species
R1617 T2800 T2798 pobj species,of
R1618 T2801 T2800 compound mouse,species
R1619 T2802 T2788 aux has,identified
R1620 T2803 T2788 neg not,identified
R1621 T2804 T2788 auxpass been,identified
R1622 T2805 T2788 advmod yet,identified
R1623 T2806 T2788 punct .,identified
R1624 T2808 T2809 aux To,isolate
R1625 T2809 T2810 advcl isolate,sought
R1626 T2811 T2812 det the,form
R1627 T2812 T2809 dobj form,isolate
R1628 T2813 T2812 amod long,form
R1629 T2814 T2812 prep of,form
R1630 T2815 T2816 compound mouse,ADAM22
R1631 T2816 T2817 compound ADAM22,transcripts
R1632 T2817 T2814 pobj transcripts,of
R1633 T2818 T2810 punct ", ",sought
R1634 T2819 T2810 prep at,sought
R1635 T2820 T2819 pcomp first,at
R1636 T2821 T2810 punct ", ",sought
R1637 T2822 T2810 nsubj we,sought
R1638 T2823 T2824 det the,genome
R1639 T2824 T2810 dobj genome,sought
R1640 T2825 T2824 compound mouse,genome
R1641 T2826 T2810 prep by,sought
R1642 T2827 T2828 compound BLAST,homology
R1643 T2828 T2829 compound homology,search
R1644 T2829 T2826 pobj search,by
R1645 T2830 T2810 punct .,sought
R1646 T2832 T2833 compound Search,results
R1647 T2833 T2834 nsubj results,showed
R1648 T2835 T2836 mark that,contained
R1649 T2836 T2834 ccomp contained,showed
R1650 T2837 T2838 det the,genome
R1651 T2838 T2836 nsubj genome,contained
R1652 T2839 T2838 compound mouse,genome
R1653 T2840 T2841 compound contig,NT_039297
R1654 T2841 T2838 appos NT_039297,genome
R1655 T2842 T2836 dobj most,contained
R1656 T2843 T2842 prep of,most
R1657 T2844 T2845 det the,gene
R1658 T2845 T2843 pobj gene,of
R1659 T2846 T2845 compound mouse,gene
R1660 T2847 T2845 compound Adam22,gene
R1661 T2848 T2834 punct .,showed
R1662 T2850 T2851 prep In,found
R1663 T2851 T2857 ccomp found,were
R1664 T2852 T2853 det this,sequence
R1665 T2853 T2850 pobj sequence,In
R1666 T2854 T2853 compound contig,sequence
R1667 T2855 T2851 punct ", ",found
R1668 T2856 T2851 nsubj we,found
R1669 T2858 T2859 amod putative,30
R1670 T2859 T2851 dobj 30,found
R1671 T2860 T2859 nmod exons,30
R1672 T2861 T2859 cc and,30
R1673 T2862 T2859 conj 31,30
R1674 T2863 T2857 punct ", ",were
R1675 T2864 T2865 det those,sequences
R1676 T2865 T2857 nsubj sequences,were
R1677 T2866 T2867 advmod quite,similar
R1678 T2867 T2857 acomp similar,were
R1679 T2868 T2867 prep to,similar
R1680 T2869 T2870 amod human,isoform
R1681 T2870 T2868 pobj isoform,to
R1682 T2871 T2870 compound ADAM22,isoform
R1683 T2872 T2870 nummod 1,isoform
R1684 T2873 T2874 punct (,NM_021723
R1685 T2874 T2870 parataxis NM_021723,isoform
R1686 T2875 T2874 nmod GenBank,NM_021723
R1687 T2876 T2874 punct #,NM_021723
R1688 T2877 T2874 punct ),NM_021723
R1689 T2878 T2857 punct .,were
R1690 T2880 T2881 aux To,confirm
R1691 T2881 T2882 advcl confirm,designed
R1692 T2883 T2881 dobj existence,confirm
R1693 T2884 T2883 prep of,existence
R1694 T2885 T2884 pobj transcripts,of
R1695 T2886 T2882 punct ", ",designed
R1696 T2887 T2882 nsubj we,designed
R1697 T2888 T2882 dobj primers,designed
R1698 T2889 T2888 prep on,primers
R1699 T2890 T2891 det the,exon
R1700 T2891 T2889 pobj exon,on
R1701 T2892 T2891 amod putative,exon
R1702 T2893 T2891 nummod 31,exon
R1703 T2894 T2882 cc and,designed
R1704 T2895 T2882 conj performed,designed
R1705 T2896 T2897 compound RT,PCR
R1706 T2897 T2895 dobj PCR,performed
R1707 T2898 T2897 punct -,PCR
R1708 T2899 T2895 advcl using,performed
R1709 T2900 T2901 compound mouse,mRNA
R1710 T2901 T2899 dobj mRNA,using
R1711 T2902 T2901 compound cerebellum,mRNA
R1712 T2903 T2899 prep as,using
R1713 T2904 T2905 det a,template
R1714 T2905 T2903 pobj template,as
R1715 T2906 T2882 punct .,designed
R1716 T2908 T2909 compound TA,cloning
R1717 T2909 T2911 nsubj cloning,showed
R1718 T2910 T2909 punct -,cloning
R1719 T2912 T2909 prep of,cloning
R1720 T2913 T2914 det the,fragments
R1721 T2914 T2912 pobj fragments,of
R1722 T2915 T2914 amod amplified,fragments
R1723 T2916 T2909 cc and,cloning
R1724 T2917 T2918 compound sequencing,analysis
R1725 T2918 T2909 conj analysis,cloning
R1726 T2919 T2920 mark that,were
R1727 T2920 T2911 ccomp were,showed
R1728 T2921 T2922 quantmod 5,8
R1729 T2922 T2924 nummod 8,clones
R1730 T2923 T2922 quantmod of,8
R1731 T2924 T2920 nsubj clones,were
R1732 T2925 T2926 nmod type,isoform
R1733 T2926 T2920 attr isoform,were
R1734 T2927 T2925 nummod 1,type
R1735 T2928 T2929 punct (,G01
R1736 T2929 T2920 parataxis G01,were
R1737 T2930 T2929 punct ),G01
R1738 T2931 T2920 punct ", ",were
R1739 T2932 T2933 nummod 2,clones
R1740 T2933 T2934 nsubj clones,were
R1741 T2934 T2920 conj were,were
R1742 T2935 T2936 nmod type,isoform
R1743 T2936 T2934 attr isoform,were
R1744 T2937 T2935 nummod 3,type
R1745 T2938 T2939 punct (,G03
R1746 T2939 T2934 parataxis G03,were
R1747 T2940 T2939 punct ),G03
R1748 T2941 T2934 cc and,were
R1749 T2942 T2943 nummod 1,clone
R1750 T2943 T2944 nsubj clone,contained
R1751 T2944 T2934 conj contained,were
R1752 T2945 T2946 punct (,G08
R1753 T2946 T2943 parataxis G08,clone
R1754 T2947 T2946 punct ),G08
R1755 T2948 T2949 det a,exon
R1756 T2949 T2944 dobj exon,contained
R1757 T2950 T2949 amod novel,exon
R1758 T2951 T2949 prep between,exon
R1759 T2952 T2953 nmod exons,29
R1760 T2953 T2951 pobj 29,between
R1761 T2954 T2953 cc and,29
R1762 T2955 T2953 conj 30,29
R1763 T2956 T2911 punct .,showed
R1764 T2958 T2959 advmod Next,performed
R1765 T2960 T2959 punct ", ",performed
R1766 T2961 T2959 nsubj we,performed
R1767 T2962 T2963 compound RT,PCR
R1768 T2963 T2965 compound PCR,analysis
R1769 T2964 T2963 punct -,PCR
R1770 T2965 T2959 dobj analysis,performed
R1771 T2966 T2965 prep of,analysis
R1772 T2967 T2966 pobj mRNAs,of
R1773 T2968 T2967 acl purified,mRNAs
R1774 T2969 T2968 prep from,purified
R1775 T2970 T2971 amod several,tissues
R1776 T2971 T2969 pobj tissues,from
R1777 T2972 T2971 amod adult,tissues
R1778 T2973 T2971 compound mouse,tissues
R1779 T2974 T2971 punct ", ",tissues
R1780 T2975 T2976 amod such,as
R1781 T2976 T2971 prep as,tissues
R1782 T2977 T2978 det the,cord
R1783 T2978 T2976 pobj cord,as
R1784 T2979 T2978 amod spinal,cord
R1785 T2980 T2978 punct ", ",cord
R1786 T2981 T2982 amod dorsal,root
R1787 T2982 T2983 compound root,ganglion
R1788 T2983 T2978 conj ganglion,cord
R1789 T2984 T2983 punct (,ganglion
R1790 T2985 T2983 appos DRG,ganglion
R1791 T2986 T2983 punct ),ganglion
R1792 T2987 T2983 punct ", ",ganglion
R1793 T2988 T2989 amod sciatic,nerve
R1794 T2989 T2983 conj nerve,ganglion
R1795 T2990 T2989 cc and,nerve
R1796 T2991 T2992 amod cultured,cells
R1797 T2992 T2989 conj cells,nerve
R1798 T2993 T2992 amod primary,cells
R1799 T2994 T2992 compound Schwann,cells
R1800 T2995 T2959 punct .,performed
R1801 T2997 T2998 advmod Interestingly,amplified
R1802 T2999 T2998 punct ", ",amplified
R1803 T3000 T3001 amod multiple,fragments
R1804 T3001 T2998 nsubjpass fragments,amplified
R1805 T3002 T3001 compound DNA,fragments
R1806 T3003 T2998 auxpass were,amplified
R1807 T3004 T2998 prep from,amplified
R1808 T3005 T3006 det each,tissue
R1809 T3006 T3004 pobj tissue,from
R1810 T3007 T3008 punct (,Fig.
R1811 T3008 T2998 parataxis Fig.,amplified
R1812 T3009 T3008 nummod 8,Fig.
R1813 T3010 T3008 punct ),Fig.
R1814 T3011 T2998 punct ", ",amplified
R1815 T3012 T2998 cc and,amplified
R1816 T3013 T3014 nsubj length,was
R1817 T3014 T2998 conj was,amplified
R1818 T3015 T3013 prep of,length
R1819 T3016 T3017 amod major,fragments
R1820 T3017 T3015 pobj fragments,of
R1821 T3018 T3013 prep in,length
R1822 T3019 T3020 det each,lane
R1823 T3020 T3018 pobj lane,in
R1824 T3021 T3014 acomp different,was
R1825 T3022 T3014 punct .,was
R1826 T3024 T3025 prep For,were
R1827 T3026 T3024 pobj example,For
R1828 T3027 T3025 punct ", ",were
R1829 T3028 T3025 nsubj length,were
R1830 T3029 T3028 prep of,length
R1831 T3030 T3031 amod major,fragments
R1832 T3031 T3029 pobj fragments,of
R1833 T3032 T3031 amod amplified,fragments
R1834 T3033 T3031 compound PCR,fragments
R1835 T3034 T3031 prep from,fragments
R1836 T3035 T3036 det the,nerve
R1837 T3036 T3034 pobj nerve,from
R1838 T3037 T3036 amod sciatic,nerve
R1839 T3038 T3039 punct (,lane
R1840 T3039 T3036 parataxis lane,nerve
R1841 T3040 T3041 compound Fig.,8A
R1842 T3041 T3039 dep 8A,lane
R1843 T3042 T3039 punct ", ",lane
R1844 T3043 T3039 nummod 5,lane
R1845 T3044 T3039 punct ),lane
R1846 T3045 T3036 cc and,nerve
R1847 T3046 T3047 amod cultured,cells
R1848 T3047 T3036 conj cells,nerve
R1849 T3048 T3047 compound Schwann,cells
R1850 T3049 T3050 punct (,lane
R1851 T3050 T3047 parataxis lane,cells
R1852 T3051 T3050 nummod 6,lane
R1853 T3052 T3050 punct ),lane
R1854 T3053 T3054 advmod almost,identical
R1855 T3054 T3025 acomp identical,were
R1856 T3055 T3025 punct ", ",were
R1857 T3056 T3025 cc and,were
R1858 T3057 T3025 conj were,were
R1859 T3058 T3057 acomp shorter,were
R1860 T3059 T3058 prep than,shorter
R1861 T3060 T3059 pobj those,than
R1862 T3061 T3060 prep from,those
R1863 T3062 T3063 amod other,tissues
R1864 T3063 T3061 pobj tissues,from
R1865 T3064 T3025 punct .,were
R1866 T3066 T3067 prep In,were
R1867 T3068 T3066 pobj contrast,In
R1868 T3069 T3067 punct ", ",were
R1869 T3070 T3071 amod major,fragments
R1870 T3071 T3067 nsubj fragments,were
R1871 T3072 T3071 prep from,fragments
R1872 T3073 T3074 det the,DRG
R1873 T3074 T3072 pobj DRG,from
R1874 T3075 T3076 punct (,lane
R1875 T3076 T3071 parataxis lane,fragments
R1876 T3077 T3076 nummod 4,lane
R1877 T3078 T3076 punct ),lane
R1878 T3079 T3067 acomp longer,were
R1879 T3080 T3079 prep than,longer
R1880 T3081 T3080 pobj others,than
R1881 T3082 T3067 punct .,were
R1882 T3084 T3085 nsubj We,performed
R1883 T3086 T3085 dobj TA,performed
R1884 T3087 T3086 punct -,TA
R1885 T3088 T3086 amod cloning,TA
R1886 T3089 T3086 cc and,TA
R1887 T3090 T3086 conj sequencing,TA
R1888 T3091 T3092 aux to,analyse
R1889 T3092 T3085 advcl analyse,performed
R1890 T3093 T3094 compound exon,organization
R1891 T3094 T3092 dobj organization,analyse
R1892 T3095 T3094 prep of,organization
R1893 T3096 T3097 det the,transcripts
R1894 T3097 T3095 pobj transcripts,of
R1895 T3098 T3097 amod amplified,transcripts
R1896 T3099 T3100 punct (,8C
R1897 T3100 T3092 parataxis 8C,analyse
R1898 T3101 T3100 compound Fig.,8C
R1899 T3102 T3100 punct ),8C
R1900 T3103 T3085 punct .,performed
R1901 T3105 T3106 compound Forty,seven
R1902 T3106 T3107 nummod seven,clones
R1903 T3107 T3108 nsubjpass clones,isolated
R1904 T3109 T3108 auxpass were,isolated
R1905 T3110 T3108 prep in,isolated
R1906 T3111 T3110 amod total,in
R1907 T3112 T3108 cc and,isolated
R1908 T3113 T3114 auxpass were,classified
R1909 T3114 T3108 conj classified,isolated
R1910 T3115 T3114 prep in,classified
R1911 T3116 T3117 nummod 16,clones
R1912 T3117 T3115 pobj clones,in
R1913 T3118 T3117 amod independent,clones
R1914 T3119 T3120 punct (,G01
R1915 T3120 T3114 parataxis G01,classified
R1916 T3121 T3122 punct –,G23
R1917 T3122 T3120 prep G23,G01
R1918 T3123 T3120 punct ),G01
R1919 T3124 T3108 punct .,isolated
R1920 T3126 T3127 nsubj Comparison,revealed
R1921 T3128 T3126 prep with,Comparison
R1922 T3129 T3130 det the,sequence
R1923 T3130 T3128 pobj sequence,with
R1924 T3131 T3130 amod genomic,sequence
R1925 T3132 T3133 det the,existence
R1926 T3133 T3127 dobj existence,revealed
R1927 T3134 T3133 prep of,existence
R1928 T3135 T3136 nummod 8,exons
R1929 T3136 T3134 pobj exons,of
R1930 T3137 T3136 amod novel,exons
R1931 T3138 T3136 punct (,exons
R1932 T3139 T3140 compound exons,27S
R1933 T3140 T3136 appos 27S,exons
R1934 T3141 T3140 punct ", ",27S
R1935 T3142 T3140 conj 27L,27S
R1936 T3143 T3142 punct ", ",27L
R1937 T3144 T3142 nummod 29.1,27L
R1938 T3145 T3142 punct ", ",27L
R1939 T3146 T3142 nummod 29.3,27L
R1940 T3147 T3142 punct ", ",27L
R1941 T3148 T3142 nummod 29.5,27L
R1942 T3149 T3142 punct ", ",27L
R1943 T3150 T3142 nummod 29.7,27L
R1944 T3151 T3142 punct ", ",27L
R1945 T3152 T3142 nummod 30,27L
R1946 T3153 T3142 cc and,27L
R1947 T3154 T3142 conj 31,27L
R1948 T3155 T3127 punct ),revealed
R1949 T3156 T3127 punct .,revealed
R1950 T3158 T3159 det These,exons
R1951 T3159 T3161 nsubjpass exons,flanked
R1952 T3160 T3159 amod novel,exons
R1953 T3162 T3161 auxpass were,flanked
R1954 T3163 T3161 agent by,flanked
R1955 T3164 T3165 advmod well,defined
R1956 T3165 T3167 amod defined,introns
R1957 T3166 T3165 punct -,defined
R1958 T3167 T3163 pobj introns,by
R1959 T3168 T3169 dep that,obey
R1960 T3169 T3167 relcl obey,introns
R1961 T3170 T3171 det the,rule
R1962 T3171 T3169 dobj rule,obey
R1963 T3172 T3173 compound GT,AG
R1964 T3173 T3171 compound AG,rule
R1965 T3174 T3173 punct -,AG
R1966 T3175 T3161 punct .,flanked
R1967 T3177 T3178 compound Exon,organization
R1968 T3178 T3179 nsubjpass organization,shown
R1969 T3180 T3178 prep of,organization
R1970 T3181 T3182 det each,clone
R1971 T3182 T3180 pobj clone,of
R1972 T3183 T3179 auxpass was,shown
R1973 T3184 T3179 prep in,shown
R1974 T3185 T3186 compound Fig.,8C
R1975 T3186 T3184 pobj 8C,in
R1976 T3187 T3179 punct .,shown
R1977 T3189 T3190 amod Characteristic,feature
R1978 T3190 T3191 nsubj feature,is
R1979 T3192 T3190 prep of,feature
R1980 T3193 T3194 compound ADAM22,transcripts
R1981 T3194 T3192 pobj transcripts,of
R1982 T3195 T3196 npadvmod tissue,specific
R1983 T3196 T3197 amod specific,insertion
R1984 T3197 T3191 attr insertion,is
R1985 T3198 T3197 cc or,insertion
R1986 T3199 T3197 conj skipping,insertion
R1987 T3200 T3199 prep of,skipping
R1988 T3201 T3200 pobj exons,of
R1989 T3202 T3191 punct .,is
R1990 T3204 T3205 mark Because,is
R1991 T3205 T3223 advcl is,change
R1992 T3206 T3207 det the,number
R1993 T3207 T3205 nsubj number,is
R1994 T3208 T3207 prep of,number
R1995 T3209 T3208 pobj nucleotides,of
R1996 T3210 T3209 prep of,nucleotides
R1997 T3211 T3212 nmod exons,26
R1998 T3212 T3210 pobj 26,of
R1999 T3213 T3212 punct ", ",26
R2000 T3214 T3212 conj 27,26
R2001 T3215 T3214 punct ", ",27
R2002 T3216 T3214 conj 27L,27
R2003 T3217 T3216 punct ", ",27L
R2004 T3218 T3216 conj 29.3,27L
R2005 T3219 T3218 punct ", ",29.3
R2006 T3220 T3218 conj 29.5,29.3
R2007 T3221 T3220 cc and,29.5
R2008 T3222 T3220 conj 29.7,29.5
R2009 T3224 T3225 det a,multiple
R2010 T3225 T3205 attr multiple,is
R2011 T3226 T3225 prep of,multiple
R2012 T3227 T3226 pobj 3,of
R2013 T3228 T3223 punct ", ",change
R2014 T3229 T3223 nsubj insertion,change
R2015 T3230 T3229 cc or,insertion
R2016 T3231 T3229 conj skipping,insertion
R2017 T3232 T3229 prep of,insertion
R2018 T3233 T3234 det these,exons
R2019 T3234 T3232 pobj exons,of
R2020 T3235 T3223 aux will,change
R2021 T3236 T3223 neg not,change
R2022 T3237 T3238 det the,frame
R2023 T3238 T3223 dobj frame,change
R2024 T3239 T3238 amod downstream,frame
R2025 T3240 T3238 compound reading,frame
R2026 T3241 T3223 punct .,change
R2027 T3243 T3244 amod Known,motives
R2028 T3244 T3246 nsubjpass motives,found
R2029 T3245 T3244 compound peptide,motives
R2030 T3247 T3246 auxpass were,found
R2031 T3248 T3246 neg not,found
R2032 T3249 T3246 prep in,found
R2033 T3250 T3251 det the,sequences
R2034 T3251 T3249 pobj sequences,in
R2035 T3252 T3253 advmod newly,isolated
R2036 T3253 T3251 amod isolated,sequences
R2037 T3254 T3246 punct .,found
R2038 T3256 T3257 det This,analysis
R2039 T3257 T3261 nsubjpass analysis,summarized
R2040 T3258 T3259 compound RT,PCR
R2041 T3259 T3257 compound PCR,analysis
R2042 T3260 T3259 punct -,PCR
R2043 T3261 T3263 ccomp summarized,subdivided
R2044 T3262 T3261 auxpass is,summarized
R2045 T3264 T3265 mark as,follows
R2046 T3265 T3261 advcl follows,summarized
R2047 T3266 T3263 punct : ,subdivided
R2048 T3267 T3268 compound ADAM22,transcripts
R2049 T3268 T3263 nsubjpass transcripts,subdivided
R2050 T3269 T3263 auxpass were,subdivided
R2051 T3270 T3263 advmod roughly,subdivided
R2052 T3271 T3263 prep in,subdivided
R2053 T3272 T3273 nummod 3,groups
R2054 T3273 T3271 pobj groups,in
R2055 T3274 T3273 punct ", ",groups
R2056 T3275 T3276 compound CNS,form
R2057 T3276 T3273 appos form,groups
R2058 T3277 T3276 punct -,form
R2059 T3278 T3276 punct (,form
R2060 T3279 T3276 acl containing,form
R2061 T3280 T3279 dobj exon,containing
R2062 T3281 T3280 nummod 27,exon
R2063 T3282 T3276 punct ),form
R2064 T3283 T3276 punct ", ",form
R2065 T3284 T3285 compound DRG,form
R2066 T3285 T3276 conj form,form
R2067 T3286 T3285 punct -,form
R2068 T3287 T3285 punct (,form
R2069 T3288 T3285 acl containing,form
R2070 T3289 T3290 compound exon,27L
R2071 T3290 T3288 dobj 27L,containing
R2072 T3291 T3285 punct ),form
R2073 T3292 T3285 cc and,form
R2074 T3293 T3294 compound Schwann,cell
R2075 T3294 T3296 compound cell,form
R2076 T3295 T3294 punct -,cell
R2077 T3296 T3285 conj form,form
R2078 T3297 T3296 punct -,form
R2079 T3298 T3296 punct (,form
R2080 T3299 T3296 acl skipping,form
R2081 T3300 T3301 nmod exon,26
R2082 T3301 T3299 dobj 26,skipping
R2083 T3302 T3301 cc and,26
R2084 T3303 T3301 conj 27,26
R2085 T3304 T3263 punct ),subdivided
R2086 T3305 T3263 punct .,subdivided
R2087 T3307 T3308 prep From,concluded
R2088 T3309 T3310 det these,results
R2089 T3310 T3307 pobj results,From
R2090 T3311 T3308 punct ", ",concluded
R2091 T3312 T3308 nsubj we,concluded
R2092 T3313 T3314 mark that,expressed
R2093 T3314 T3308 ccomp expressed,concluded
R2094 T3315 T3314 nsubjpass ADAM22,expressed
R2095 T3316 T3314 auxpass is,expressed
R2096 T3317 T3314 prep in,expressed
R2097 T3318 T3319 det a,manner
R2098 T3319 T3317 pobj manner,in
R2099 T3320 T3321 compound cell,type
R2100 T3321 T3323 npadvmod type,specific
R2101 T3322 T3321 punct -,type
R2102 T3323 T3319 amod specific,manner
R2103 T3324 T3314 punct ", ",expressed
R2104 T3325 T3314 cc and,expressed
R2105 T3326 T3314 conj plays,expressed
R2106 T3327 T3328 det an,role
R2107 T3328 T3326 dobj role,plays
R2108 T3329 T3328 amod essential,role
R2109 T3330 T3326 prep in,plays
R2110 T3331 T3330 pobj myelinogenesis,in
R2111 T3332 T3326 prep in,plays
R2112 T3333 T3334 det the,PNS
R2113 T3334 T3332 pobj PNS,in
R2114 T3335 T3308 punct .,concluded
R667 T1244 T1243 prep of,Generation
R668 T1245 T1246 npadvmod ADAM22,deficient
R669 T1246 T1248 amod deficient,mice
R670 T1247 T1246 punct -,deficient
R671 T1248 T1244 pobj mice,of
R672 T1250 T1251 nsubjpass Mice,generated
R673 T1252 T1250 acl carrying,Mice
R674 T1253 T1254 det a,mutation
R675 T1254 T1252 dobj mutation,carrying
R676 T1255 T1254 amod targeted,mutation
R677 T1256 T1252 prep in,carrying
R678 T1257 T1258 poss their,gene
R679 T1258 T1256 pobj gene,in
R680 T1259 T1258 compound Adam22,gene
R681 T1260 T1251 auxpass were,generated
R682 T1261 T1251 prep by,generated
R683 T1262 T1263 amod homologous,recombination
R684 T1263 T1261 pobj recombination,by
R685 T1264 T1265 punct (,1A
R686 T1265 T1251 parataxis 1A,generated
R687 T1266 T1265 compound Fig.,1A
R688 T1267 T1265 punct ),1A
R689 T1268 T1251 punct .,generated
R690 T1270 T1271 amod Correct,events
R691 T1271 T1273 nsubjpass events,confirmed
R692 T1272 T1271 amod targeting,events
R693 T1274 T1273 auxpass were,confirmed
R694 T1275 T1273 prep by,confirmed
R695 T1276 T1277 compound Southern,blot
R696 T1277 T1278 compound blot,analysis
R697 T1278 T1275 pobj analysis,by
R698 T1279 T1280 punct (,1B
R699 T1280 T1273 parataxis 1B,confirmed
R700 T1281 T1280 compound Fig.,1B
R701 T1282 T1280 punct ),1B
R702 T1283 T1273 punct .,confirmed
R703 T1285 T1286 mark Since,introduced
R704 T1286 T1291 advcl introduced,synthesized
R705 T1287 T1288 det the,codon
R706 T1288 T1286 nsubjpass codon,introduced
R707 T1289 T1288 compound termination,codon
R708 T1290 T1286 auxpass was,introduced
R709 T1292 T1286 prep in,introduced
R710 T1293 T1292 pobj exon,in
R711 T1294 T1293 nummod 8,exon
R712 T1295 T1286 prep in,introduced
R713 T1296 T1297 det the,domain
R714 T1297 T1295 pobj domain,in
R715 T1298 T1297 amod pro-protein,domain
R716 T1299 T1291 punct ", ",synthesized
R717 T1300 T1301 advmod only,form
R718 T1301 T1291 nsubjpass form,synthesized
R719 T1302 T1301 det the,form
R720 T1303 T1301 amod truncated,form
R721 T1304 T1301 prep of,form
R722 T1305 T1306 det the,protein
R723 T1306 T1304 pobj protein,of
R724 T1307 T1306 compound ADAM22,protein
R725 T1308 T1291 aux would,synthesized
R726 T1309 T1291 auxpass be,synthesized
R727 T1310 T1291 prep from,synthesized
R728 T1311 T1312 det this,allele
R729 T1312 T1310 pobj allele,from
R730 T1313 T1312 amod targeted,allele
R731 T1314 T1291 punct .,synthesized
R732 T1316 T1317 mark Because,removed
R733 T1317 T1323 advcl removed,considered
R734 T1318 T1319 det a,domain
R735 T1319 T1317 nsubjpass domain,removed
R736 T1320 T1319 amod pro-protein,domain
R737 T1321 T1317 auxpass is,removed
R738 T1322 T1317 advmod always,removed
R739 T1324 T1317 prep in,removed
R740 T1325 T1326 det the,proteins
R741 T1326 T1324 pobj proteins,in
R742 T1327 T1326 amod mature,proteins
R743 T1328 T1326 amod functional,proteins
R744 T1329 T1326 compound ADAM,proteins
R745 T1330 T1326 punct -,proteins
R746 T1331 T1317 agent by,removed
R747 T1332 T1333 det the,proteases
R748 T1333 T1331 pobj proteases,by
R749 T1334 T1335 npadvmod Furin,like
R750 T1335 T1333 amod like,proteases
R751 T1336 T1335 punct -,like
R752 T1337 T1317 cc and,removed
R753 T1338 T1339 auxpass is,thought
R754 T1339 T1317 conj thought,removed
R755 T1340 T1341 aux to,be
R756 T1341 T1339 xcomp be,thought
R757 T1342 T1341 acomp non-functional,be
R758 T1343 T1341 npadvmod itself,be
R759 T1344 T1323 punct ", ",considered
R760 T1345 T1323 nsubj we,considered
R761 T1346 T1347 mark that,has
R762 T1347 T1323 ccomp has,considered
R763 T1348 T1349 det this,form
R764 T1349 T1347 nsubj form,has
R765 T1350 T1349 amod truncated,form
R766 T1351 T1349 prep of,form
R767 T1352 T1353 compound ADAM22,protein
R768 T1353 T1351 pobj protein,of
R769 T1354 T1355 det no,function
R770 T1355 T1347 dobj function,has
R771 T1356 T1323 punct .,considered
R772 T1358 T1359 nsubjpass Absence,confirmed
R773 T1360 T1358 prep of,Absence
R774 T1361 T1362 amod mature,protein
R775 T1362 T1360 pobj protein,of
R776 T1363 T1362 compound ADAM22,protein
R777 T1364 T1358 prep in,Absence
R778 T1365 T1366 amod homozygous,mutants
R779 T1366 T1364 pobj mutants,in
R780 T1367 T1359 auxpass was,confirmed
R781 T1368 T1359 prep by,confirmed
R782 T1369 T1370 compound Western,blot
R783 T1370 T1371 compound blot,analysis
R784 T1371 T1368 pobj analysis,by
R785 T1372 T1371 acl using,analysis
R786 T1373 T1374 det the,antibody
R787 T1374 T1372 dobj antibody,using
R788 T1375 T1374 amod specific,antibody
R789 T1376 T1374 punct ", ",antibody
R790 T1377 T1378 dep which,recognizes
R791 T1378 T1374 relcl recognizes,antibody
R792 T1379 T1380 det the,domain
R793 T1380 T1378 dobj domain,recognizes
R794 T1381 T1380 amod cytoplasmic,domain
R795 T1382 T1380 prep of,domain
R796 T1383 T1384 det the,protein
R797 T1384 T1382 pobj protein,of
R798 T1385 T1384 compound ADAM22,protein
R799 T1386 T1387 punct (,1C
R800 T1387 T1359 parataxis 1C,confirmed
R801 T1388 T1387 compound Fig.,1C
R802 T1389 T1387 punct ),1C
R803 T1390 T1359 punct .,confirmed
R804 T1392 T1393 amod Homozygous,mutants
R805 T1393 T1394 nsubj mutants,showed
R806 T1395 T1396 det no,defects
R807 T1396 T1394 dobj defects,showed
R808 T1397 T1396 amod noticeable,defects
R809 T1398 T1394 prep at,showed
R810 T1399 T1398 pobj birth,at
R811 T1400 T1394 cc and,showed
R812 T1401 T1394 conj were,showed
R813 T1402 T1401 acomp indistinguishable,were
R814 T1403 T1402 prep from,indistinguishable
R815 T1404 T1405 amod wild,type
R816 T1405 T1407 nmod type,littermates
R817 T1406 T1405 punct -,type
R818 T1407 T1403 pobj littermates,from
R819 T1408 T1405 cc or,type
R820 T1409 T1405 conj heterozygous,type
R821 T1410 T1401 prep during,were
R822 T1411 T1412 det the,week
R823 T1412 T1410 pobj week,during
R824 T1413 T1412 amod first,week
R825 T1414 T1394 punct .,showed
R826 T1416 T1417 prep At,were
R827 T1418 T1419 amod postnatal,day
R828 T1419 T1416 pobj day,At
R829 T1420 T1419 nummod 10,day
R830 T1421 T1419 punct (,day
R831 T1422 T1419 appos P10,day
R832 T1423 T1417 punct ),were
R833 T1424 T1417 punct ", ",were
R834 T1425 T1417 nsubj most,were
R835 T1426 T1425 prep of,most
R836 T1427 T1428 det the,mutants
R837 T1428 T1426 pobj mutants,of
R838 T1429 T1428 amod homozygous,mutants
R839 T1430 T1417 acomp distinguishable,were
R840 T1431 T1430 prep by,distinguishable
R841 T1432 T1431 pobj abnormalities,by
R842 T1433 T1434 amod such,as
R843 T1434 T1432 prep as,abnormalities
R844 T1435 T1436 amod reduced,weight
R845 T1436 T1434 pobj weight,as
R846 T1437 T1436 compound body,weight
R847 T1438 T1436 cc and,weight
R848 T1439 T1440 amod uncoordinated,movements
R849 T1440 T1436 conj movements,weight
R850 T1441 T1440 prep of,movements
R851 T1442 T1443 poss their,limbs
R852 T1443 T1441 pobj limbs,of
R853 T1444 T1417 punct .,were
R854 T1446 T1447 prep After,displayed
R855 T1448 T1446 pobj P10,After
R856 T1449 T1447 punct ", ",displayed
R857 T1450 T1451 det all,homozygotes
R858 T1451 T1447 nsubj homozygotes,displayed
R859 T1452 T1453 amod severe,ataxia
R860 T1453 T1447 dobj ataxia,displayed
R861 T1454 T1455 punct (,Fig.
R862 T1455 T1447 parataxis Fig.,displayed
R863 T1456 T1455 nummod 2,Fig.
R864 T1457 T1455 punct ),Fig.
R865 T1458 T1447 cc and,displayed
R866 T1459 T1447 conj began,displayed
R867 T1460 T1461 aux to,die
R868 T1461 T1459 xcomp die,began
R869 T1462 T1447 punct .,displayed
R870 T1464 T1465 aux To,measure
R871 T1465 T1466 advcl measure,backcrossed
R872 T1467 T1468 det the,rate
R873 T1468 T1465 dobj rate,measure
R874 T1469 T1468 compound survival,rate
R875 T1470 T1468 cc and,rate
R876 T1471 T1472 compound body,weight
R877 T1472 T1468 conj weight,rate
R878 T1473 T1468 prep of,rate
R879 T1474 T1475 det each,genotype
R880 T1475 T1473 pobj genotype,of
R881 T1476 T1465 advmod precisely,measure
R882 T1477 T1466 punct ", ",backcrossed
R883 T1478 T1466 nsubj we,backcrossed
R884 T1479 T1480 amod heterozygous,mutants
R885 T1480 T1466 dobj mutants,backcrossed
R886 T1481 T1480 amod male,mutants
R887 T1482 T1466 prep to,backcrossed
R888 T1483 T1484 nmod C57BL,females
R889 T1484 T1482 pobj females,to
R890 T1485 T1483 punct /,C57BL
R891 T1486 T1483 nummod 6,C57BL
R892 T1487 T1488 amod more,6
R893 T1488 T1490 nummod 6,times
R894 T1489 T1488 quantmod than,6
R895 T1490 T1466 npadvmod times,backcrossed
R896 T1491 T1466 punct .,backcrossed
R897 T1493 T1494 det The,males
R898 T1494 T1501 nsubjpass males,intercrossed
R899 T1495 T1494 amod resulting,males
R900 T1496 T1497 punct (,N
R901 T1497 T1494 parataxis N,males
R902 T1498 T1497 nummod 6,N
R903 T1499 T1497 punct ),N
R904 T1500 T1494 amod heterozygous,males
R905 T1502 T1494 cc and,males
R906 T1503 T1494 conj females,males
R907 T1504 T1501 auxpass were,intercrossed
R908 T1505 T1501 cc and,intercrossed
R909 T1506 T1507 det the,offspring
R910 T1507 T1509 nsubjpass offspring,analysed
R911 T1508 T1507 amod produced,offspring
R912 T1509 T1501 conj analysed,intercrossed
R913 T1510 T1509 auxpass were,analysed
R914 T1511 T1509 punct .,analysed
R915 T1513 T1514 det The,numbers
R916 T1514 T1515 nsubjpass numbers,shown
R917 T1516 T1514 prep of,numbers
R918 T1517 T1516 pobj survivors,of
R919 T1518 T1517 prep of,survivors
R920 T1519 T1520 det each,genotype
R921 T1520 T1518 pobj genotype,of
R922 T1521 T1522 det every,days
R923 T1522 T1514 npadvmod days,numbers
R924 T1523 T1522 nummod 5,days
R925 T1524 T1515 auxpass are,shown
R926 T1525 T1515 prep in,shown
R927 T1526 T1525 pobj Table,in
R928 T1527 T1526 nummod 1,Table
R929 T1528 T1515 punct .,shown
R930 T1530 T1531 prep At,was
R931 T1532 T1530 pobj P10,At
R932 T1533 T1531 punct ", ",was
R933 T1534 T1535 det the,ratio
R934 T1535 T1531 nsubj ratio,was
R935 T1536 T1535 prep of,ratio
R936 T1537 T1538 det each,genotype
R937 T1538 T1536 pobj genotype,of
R938 T1539 T1531 prep in,was
R939 T1540 T1541 amod close,accordance
R940 T1541 T1539 pobj accordance,in
R941 T1542 T1541 prep with,accordance
R942 T1543 T1544 det the,ratio
R943 T1544 T1542 pobj ratio,with
R944 T1545 T1544 amod Mendelian,ratio
R945 T1546 T1547 punct (,%
R946 T1547 T1541 parataxis %,accordance
R947 T1548 T1547 nummod 20.5,%
R948 T1549 T1547 punct +,%
R949 T1550 T1547 punct /,%
R950 T1551 T1547 punct +,%
R951 T1552 T1547 punct ", ",%
R952 T1553 T1554 nummod 55.1,%
R953 T1554 T1547 appos %,%
R954 T1555 T1554 punct +,%
R955 T1556 T1554 punct /,%
R956 T1557 T1554 punct -,%
R957 T1558 T1547 punct ", ",%
R958 T1559 T1560 nummod 24.4,%
R959 T1560 T1547 appos %,%
R960 T1561 T1560 punct -,%
R961 T1562 T1560 punct /,%
R962 T1563 T1560 punct -,%
R963 T1564 T1547 punct ;,%
R964 T1565 T1566 nsubj n,78
R965 T1566 T1547 ccomp 78,%
R966 T1567 T1566 punct =,78
R967 T1568 T1547 punct ),%
R968 T1569 T1531 punct .,was
R969 T1571 T1572 det This,result
R970 T1572 T1573 nsubj result,shows
R971 T1574 T1575 mark that,is
R972 T1575 T1573 ccomp is,shows
R973 T1576 T1575 nsubj ADAM22,is
R974 T1577 T1575 neg not,is
R975 T1578 T1575 acomp essential,is
R976 T1579 T1578 prep for,essential
R977 T1580 T1579 pobj embryogenesis,for
R978 T1581 T1573 punct .,shows
R979 T1583 T1584 prep At,was
R980 T1585 T1583 pobj P10,At
R981 T1586 T1584 punct ", ",was
R982 T1587 T1588 det the,weight
R983 T1588 T1584 nsubj weight,was
R984 T1589 T1588 amod average,weight
R985 T1590 T1588 compound body,weight
R986 T1591 T1588 prep of,weight
R987 T1592 T1593 amod homozygous,mutants
R988 T1593 T1591 pobj mutants,of
R989 T1594 T1595 advmod approximately,half
R990 T1595 T1584 attr half,was
R991 T1596 T1595 det that,half
R992 T1597 T1595 prep of,half
R993 T1598 T1599 amod wild,type
R994 T1599 T1601 nmod type,littermates
R995 T1600 T1599 punct -,type
R996 T1601 T1597 pobj littermates,of
R997 T1602 T1599 cc and,type
R998 T1603 T1599 conj heterozygous,type
R999 T1604 T1605 punct (,Table