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PMC:1142324 / 20699-21474 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T5985 0-5 JJ denotes Adult
T5987 6-11 NN denotes C57BL
T5988 11-12 HYPH denotes /
T5989 12-13 CD denotes 6
T5990 14-18 JJ denotes male
T5986 19-23 NNS denotes mice
T5992 24-28 VBD denotes were
T5991 29-33 VBN denotes used
T5993 34-36 IN denotes in
T5994 37-41 DT denotes this
T5995 42-47 NN denotes study
T5996 47-48 . denotes .
T5997 48-451 sentence denotes Total RNAs purified from the cerebellum, spinal cord, sciatic nerve, DRG and cultured Schwann cells using TRIzol (Invitrogen Corp.) and RNeasy kit (Qiagen GmbH) were analysed by RT-PCR using SuperScript II and random primer (Invitrogen Corp.), and PCR amplification (40 cycles; 94°C-30 s, 60°C-30 s and 68°C-5 min) with Expand Hi-Fidelity DNA polymerase (Roche Diagnostics) and ADAM22-specific primers.
T5998 49-54 JJ denotes Total
T5999 55-59 NNS denotes RNAs
T6001 60-68 VBN denotes purified
T6002 69-73 IN denotes from
T6003 74-77 DT denotes the
T6004 78-88 NN denotes cerebellum
T6005 88-90 , denotes ,
T6006 90-96 JJ denotes spinal
T6007 97-101 NN denotes cord
T6008 101-103 , denotes ,
T6009 103-110 JJ denotes sciatic
T6010 111-116 NN denotes nerve
T6011 116-118 , denotes ,
T6012 118-121 NN denotes DRG
T6013 122-125 CC denotes and
T6014 126-134 VBN denotes cultured
T6016 135-142 NNP denotes Schwann
T6015 143-148 NNS denotes cells
T6017 149-154 VBG denotes using
T6018 155-161 NN denotes TRIzol
T6019 162-163 -LRB- denotes (
T6021 163-173 NNP denotes Invitrogen
T6020 174-179 NNP denotes Corp.
T6022 179-180 -RRB- denotes )
T6023 181-184 CC denotes and
T6024 185-191 NN denotes RNeasy
T6025 192-195 NN denotes kit
T6026 196-197 -LRB- denotes (
T6028 197-203 NNP denotes Qiagen
T6027 204-208 NNP denotes GmbH
T6029 208-209 -RRB- denotes )
T6030 210-214 VBD denotes were
T6000 215-223 VBN denotes analysed
T6031 224-226 IN denotes by
T6032 227-229 NN denotes RT
T6034 229-230 HYPH denotes -
T6033 230-233 NN denotes PCR
T6035 234-239 VBG denotes using
T6036 240-251 NNP denotes SuperScript
T6037 252-254 CD denotes II
T6038 255-258 CC denotes and
T6039 259-265 JJ denotes random
T6040 266-272 NN denotes primer
T6041 273-274 -LRB- denotes (
T6043 274-284 NNP denotes Invitrogen
T6042 285-290 NNP denotes Corp.
T6044 290-291 -RRB- denotes )
T6045 291-293 , denotes ,
T6046 293-296 CC denotes and
T6047 297-300 NN denotes PCR
T6048 301-314 NN denotes amplification
T6049 315-316 -LRB- denotes (
T6051 316-318 CD denotes 40
T6052 319-325 NNS denotes cycles
T6053 325-326 : denotes ;
T6054 327-329 CD denotes 94
T6050 329-331 NN denotes °C
T6055 331-332 HYPH denotes -
T6056 332-334 CD denotes 30
T6057 335-336 NN denotes s
T6058 336-338 , denotes ,
T6059 338-340 CD denotes 60
T6060 340-342 NN denotes °C
T6061 342-343 HYPH denotes -
T6062 343-345 CD denotes 30
T6063 346-347 NN denotes s
T6064 348-351 CC denotes and
T6065 352-354 CD denotes 68
T6066 354-356 NN denotes °C
T6067 356-357 HYPH denotes -
T6068 357-358 CD denotes 5
T6069 359-362 NN denotes min
T6070 362-363 -RRB- denotes )
T6071 364-368 IN denotes with
T6072 369-375 NNP denotes Expand
T6074 376-378 NNP denotes Hi
T6076 378-379 HYPH denotes -
T6075 379-387 NNP denotes Fidelity
T6077 388-391 NN denotes DNA
T6073 392-402 NN denotes polymerase
T6078 403-404 -LRB- denotes (
T6080 404-409 NNP denotes Roche
T6079 410-421 NNP denotes Diagnostics
T6081 421-422 -RRB- denotes )
T6082 423-426 CC denotes and
T6083 427-433 NN denotes ADAM22
T6085 433-434 HYPH denotes -
T6084 434-442 JJ denotes specific
T6086 443-450 NNS denotes primers
T6087 450-451 . denotes .
T6088 451-630 sentence denotes To detect splicing variants in the cytoplasmic domain, a forward primer was placed just upstream of the transmembrane domain and a reverse primer was set on the terminating exon.
T6089 452-454 TO denotes To
T6090 455-461 VB denotes detect
T6092 462-470 NN denotes splicing
T6093 471-479 NNS denotes variants
T6094 480-482 IN denotes in
T6095 483-486 DT denotes the
T6097 487-498 JJ denotes cytoplasmic
T6096 499-505 NN denotes domain
T6098 505-507 , denotes ,
T6099 507-508 DT denotes a
T6101 509-516 JJ denotes forward
T6100 517-523 NN denotes primer
T6102 524-527 VBD denotes was
T6091 528-534 VBN denotes placed
T6103 535-539 RB denotes just
T6104 540-548 RB denotes upstream
T6105 549-551 IN denotes of
T6106 552-555 DT denotes the
T6108 556-569 JJ denotes transmembrane
T6107 570-576 NN denotes domain
T6109 577-580 CC denotes and
T6110 581-582 DT denotes a
T6112 583-590 JJ denotes reverse
T6111 591-597 NN denotes primer
T6114 598-601 VBD denotes was
T6113 602-605 VBN denotes set
T6115 606-608 IN denotes on
T6116 609-612 DT denotes the
T6118 613-624 VBG denotes terminating
T6117 625-629 NN denotes exon
T6119 629-630 . denotes .
T6120 630-678 sentence denotes The primers used in this study were as follows.
T6121 631-634 DT denotes The
T6122 635-642 NNS denotes primers
T6124 643-647 VBN denotes used
T6125 648-650 IN denotes in
T6126 651-655 DT denotes this
T6127 656-661 NN denotes study
T6123 662-666 VBD denotes were
T6128 667-669 IN denotes as
T6129 670-677 VBZ denotes follows
T6130 677-678 . denotes .
T6131 678-775 sentence denotes ISH04-forward: 5'-AACAGGCACTGGACAGGGGCTGAC-3' and ISH04-reverse: 5'-AATGGATGTCTCCCATAGCCTGGC-3'.
T6132 679-684 NN denotes ISH04
T6133 684-685 HYPH denotes -
T6134 685-692 JJ denotes forward
T6135 692-694 : denotes :
T6136 694-695 CD denotes 5
T6138 695-696 SYM denotes '
T6139 696-697 HYPH denotes -
T6137 697-721 NN denotes AACAGGCACTGGACAGGGGCTGAC
T6140 721-722 HYPH denotes -
T6141 722-723 CD denotes 3
T6142 723-724 SYM denotes '
T6143 725-728 CC denotes and
T6144 729-734 NN denotes ISH04
T6145 734-735 HYPH denotes -
T6146 735-742 JJ denotes reverse
T6147 742-744 : denotes :
T6148 744-745 CD denotes 5
T6150 745-746 SYM denotes '
T6151 746-747 HYPH denotes -
T6149 747-771 NN denotes AATGGATGTCTCCCATAGCCTGGC
T6152 771-772 HYPH denotes -
T6153 772-773 CD denotes 3
T6154 773-774 SYM denotes '
T6155 774-775 . denotes .
R3758 T5985 T5986 amod Adult,mice
R3759 T5986 T5991 nsubjpass mice,used
R3760 T5987 T5986 nmod C57BL,mice
R3761 T5988 T5987 punct /,C57BL
R3762 T5989 T5987 nummod 6,C57BL
R3763 T5990 T5986 amod male,mice
R3764 T5992 T5991 auxpass were,used
R3765 T5993 T5991 prep in,used
R3766 T5994 T5995 det this,study
R3767 T5995 T5993 pobj study,in
R3768 T5996 T5991 punct .,used
R3769 T5998 T5999 amod Total,RNAs
R3770 T5999 T6000 nsubjpass RNAs,analysed
R3771 T6001 T5999 acl purified,RNAs
R3772 T6002 T6001 prep from,purified
R3773 T6003 T6004 det the,cerebellum
R3774 T6004 T6002 pobj cerebellum,from
R3775 T6005 T6004 punct ", ",cerebellum
R3776 T6006 T6007 amod spinal,cord
R3777 T6007 T6004 conj cord,cerebellum
R3778 T6008 T6007 punct ", ",cord
R3779 T6009 T6010 amod sciatic,nerve
R3780 T6010 T6007 conj nerve,cord
R3781 T6011 T6010 punct ", ",nerve
R3782 T6012 T6010 conj DRG,nerve
R3783 T6013 T6012 cc and,DRG
R3784 T6014 T6015 amod cultured,cells
R3785 T6015 T6012 conj cells,DRG
R3786 T6016 T6015 compound Schwann,cells
R3787 T6017 T6001 advcl using,purified
R3788 T6018 T6017 dobj TRIzol,using
R3789 T6019 T6020 punct (,Corp.
R3790 T6020 T6018 parataxis Corp.,TRIzol
R3791 T6021 T6020 compound Invitrogen,Corp.
R3792 T6022 T6020 punct ),Corp.
R3793 T6023 T6018 cc and,TRIzol
R3794 T6024 T6025 compound RNeasy,kit
R3795 T6025 T6018 conj kit,TRIzol
R3796 T6026 T6027 punct (,GmbH
R3797 T6027 T6025 parataxis GmbH,kit
R3798 T6028 T6027 compound Qiagen,GmbH
R3799 T6029 T6027 punct ),GmbH
R3800 T6030 T6000 auxpass were,analysed
R3801 T6031 T6000 prep by,analysed
R3802 T6032 T6033 compound RT,PCR
R3803 T6033 T6031 pobj PCR,by
R3804 T6034 T6033 punct -,PCR
R3805 T6035 T6000 advcl using,analysed
R3806 T6036 T6035 dobj SuperScript,using
R3807 T6037 T6036 nummod II,SuperScript
R3808 T6038 T6036 cc and,SuperScript
R3809 T6039 T6040 amod random,primer
R3810 T6040 T6036 conj primer,SuperScript
R3811 T6041 T6042 punct (,Corp.
R3812 T6042 T6040 parataxis Corp.,primer
R3813 T6043 T6042 compound Invitrogen,Corp.
R3814 T6044 T6042 punct ),Corp.
R3815 T6045 T6036 punct ", ",SuperScript
R3816 T6046 T6036 cc and,SuperScript
R3817 T6047 T6048 compound PCR,amplification
R3818 T6048 T6036 conj amplification,SuperScript
R3819 T6049 T6050 punct (,°C
R3820 T6050 T6048 parataxis °C,amplification
R3821 T6051 T6052 nummod 40,cycles
R3822 T6052 T6050 dep cycles,°C
R3823 T6053 T6050 punct ;,°C
R3824 T6054 T6050 nummod 94,°C
R3825 T6055 T6050 punct -,°C
R3826 T6056 T6057 nummod 30,s
R3827 T6057 T6050 npadvmod s,°C
R3828 T6058 T6050 punct ", ",°C
R3829 T6059 T6060 nummod 60,°C
R3830 T6060 T6050 conj °C,°C
R3831 T6061 T6060 punct -,°C
R3832 T6062 T6063 nummod 30,s
R3833 T6063 T6060 npadvmod s,°C
R3834 T6064 T6060 cc and,°C
R3835 T6065 T6066 nummod 68,°C
R3836 T6066 T6060 conj °C,°C
R3837 T6067 T6066 punct -,°C
R3838 T6068 T6069 nummod 5,min
R3839 T6069 T6066 npadvmod min,°C
R3840 T6070 T6050 punct ),°C
R3841 T6071 T6048 prep with,amplification
R3842 T6072 T6073 compound Expand,polymerase
R3843 T6073 T6071 pobj polymerase,with
R3844 T6074 T6075 compound Hi,Fidelity
R3845 T6075 T6073 compound Fidelity,polymerase
R3846 T6076 T6075 punct -,Fidelity
R3847 T6077 T6073 compound DNA,polymerase
R3848 T6078 T6079 punct (,Diagnostics
R3849 T6079 T6073 parataxis Diagnostics,polymerase
R3850 T6080 T6079 compound Roche,Diagnostics
R3851 T6081 T6079 punct ),Diagnostics
R3852 T6082 T6073 cc and,polymerase
R3853 T6083 T6084 npadvmod ADAM22,specific
R3854 T6084 T6086 amod specific,primers
R3855 T6085 T6084 punct -,specific
R3856 T6086 T6073 conj primers,polymerase
R3857 T6087 T6000 punct .,analysed
R3858 T6089 T6090 aux To,detect
R3859 T6090 T6091 advcl detect,placed
R3860 T6092 T6093 compound splicing,variants
R3861 T6093 T6090 dobj variants,detect
R3862 T6094 T6090 prep in,detect
R3863 T6095 T6096 det the,domain
R3864 T6096 T6094 pobj domain,in
R3865 T6097 T6096 amod cytoplasmic,domain
R3866 T6098 T6091 punct ", ",placed
R3867 T6099 T6100 det a,primer
R3868 T6100 T6091 nsubjpass primer,placed
R3869 T6101 T6100 amod forward,primer
R3870 T6102 T6091 auxpass was,placed
R3871 T6103 T6104 advmod just,upstream
R3872 T6104 T6091 advmod upstream,placed
R3873 T6105 T6104 prep of,upstream
R3874 T6106 T6107 det the,domain
R3875 T6107 T6105 pobj domain,of
R3876 T6108 T6107 amod transmembrane,domain
R3877 T6109 T6091 cc and,placed
R3878 T6110 T6111 det a,primer
R3879 T6111 T6113 nsubjpass primer,set
R3880 T6112 T6111 amod reverse,primer
R3881 T6113 T6091 conj set,placed
R3882 T6114 T6113 auxpass was,set
R3883 T6115 T6113 prep on,set
R3884 T6116 T6117 det the,exon
R3885 T6117 T6115 pobj exon,on
R3886 T6118 T6117 amod terminating,exon
R3887 T6119 T6091 punct .,placed
R3888 T6121 T6122 det The,primers
R3889 T6122 T6123 nsubj primers,were
R3890 T6124 T6122 acl used,primers
R3891 T6125 T6124 prep in,used
R3892 T6126 T6127 det this,study
R3893 T6127 T6125 pobj study,in
R3894 T6128 T6129 mark as,follows
R3895 T6129 T6123 advcl follows,were
R3896 T6130 T6123 punct .,were
R3897 T6133 T6132 punct -,ISH04
R3898 T6134 T6132 amod forward,ISH04
R3899 T6135 T6132 punct : ,ISH04
R3900 T6136 T6137 nummod 5,AACAGGCACTGGACAGGGGCTGAC
R3901 T6137 T6132 appos AACAGGCACTGGACAGGGGCTGAC,ISH04
R3902 T6138 T6136 punct ',5
R3903 T6139 T6137 punct -,AACAGGCACTGGACAGGGGCTGAC
R3904 T6140 T6137 punct -,AACAGGCACTGGACAGGGGCTGAC
R3905 T6141 T6137 nummod 3,AACAGGCACTGGACAGGGGCTGAC
R3906 T6142 T6137 punct ',AACAGGCACTGGACAGGGGCTGAC
R3907 T6143 T6132 cc and,ISH04
R3908 T6144 T6132 conj ISH04,ISH04
R3909 T6145 T6144 punct -,ISH04
R3910 T6146 T6144 amod reverse,ISH04
R3911 T6147 T6144 punct : ,ISH04
R3912 T6148 T6149 nummod 5,AATGGATGTCTCCCATAGCCTGGC
R3913 T6149 T6144 appos AATGGATGTCTCCCATAGCCTGGC,ISH04
R3914 T6150 T6148 punct ',5
R3915 T6151 T6149 punct -,AATGGATGTCTCCCATAGCCTGGC
R3916 T6152 T6149 punct -,AATGGATGTCTCCCATAGCCTGGC
R3917 T6153 T6149 nummod 3,AATGGATGTCTCCCATAGCCTGGC
R3918 T6154 T6149 punct ',AATGGATGTCTCCCATAGCCTGGC
R3919 T6155 T6144 punct .,ISH04