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PMC:1142324 / 19740-20681 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T5708 0-7 JJ denotes Primary
T5710 8-15 NNP denotes Schwann
T5709 16-21 NNS denotes cells
T5712 22-26 VBD denotes were
T5711 27-35 VBN denotes prepared
T5713 36-40 IN denotes from
T5714 41-42 CD denotes 4
T5716 42-43 HYPH denotes -
T5715 43-48 NN denotes month
T5718 48-49 HYPH denotes -
T5717 49-52 JJ denotes old
T5720 53-58 NN denotes C57BL
T5721 58-59 HYPH denotes /
T5722 59-60 CD denotes 6
T5719 61-65 NNS denotes mice
T5723 66-75 VBG denotes according
T5724 76-78 IN denotes to
T5725 79-85 NNP denotes Manent
T5727 85-87 POS denotes 's
T5726 88-96 NN denotes protocol
T5728 97-98 -LRB- denotes [
T5729 98-100 CD denotes 32
T5730 100-101 -RRB- denotes ]
T5731 102-106 IN denotes with
T5732 107-112 JJ denotes minor
T5733 113-126 NNS denotes modifications
T5734 126-127 . denotes .
T5735 127-459 sentence denotes Briefly, sciatic nerves were removed and incubated for 7 days in the pre-treatment medium, which consisted of D-MEM (high glucose) supplemented with 10% FCS, 50 mg/ml gentamicin (Invitrogen Corp.), 2.5 μg/ml fungizone (Invitrogen Corp.), 2 μM forskolin (EMD Biosciences Inc.) and 10 ng/ml recombinant heregulin-beta1 (R&D Systems).
T5736 128-135 RB denotes Briefly
T5738 135-137 , denotes ,
T5739 137-144 JJ denotes sciatic
T5740 145-151 NNS denotes nerves
T5741 152-156 VBD denotes were
T5737 157-164 VBN denotes removed
T5742 165-168 CC denotes and
T5743 169-178 VBN denotes incubated
T5744 179-182 IN denotes for
T5745 183-184 CD denotes 7
T5746 185-189 NNS denotes days
T5747 190-192 IN denotes in
T5748 193-196 DT denotes the
T5750 197-210 JJ denotes pre-treatment
T5749 211-217 NN denotes medium
T5751 217-219 , denotes ,
T5752 219-224 WDT denotes which
T5753 225-234 VBD denotes consisted
T5754 235-237 IN denotes of
T5755 238-239 NN denotes D
T5757 239-240 HYPH denotes -
T5756 240-243 NN denotes MEM
T5758 244-245 -LRB- denotes (
T5760 245-249 JJ denotes high
T5759 250-257 NN denotes glucose
T5761 257-258 -RRB- denotes )
T5762 259-271 VBN denotes supplemented
T5763 272-276 IN denotes with
T5764 277-279 CD denotes 10
T5765 279-280 NN denotes %
T5766 281-284 NN denotes FCS
T5767 284-286 , denotes ,
T5768 286-288 CD denotes 50
T5769 289-291 NN denotes mg
T5771 291-292 SYM denotes /
T5772 292-294 NN denotes ml
T5770 295-305 NN denotes gentamicin
T5773 306-307 -LRB- denotes (
T5775 307-317 NNP denotes Invitrogen
T5774 318-323 NNP denotes Corp.
T5776 323-324 -RRB- denotes )
T5777 324-326 , denotes ,
T5778 326-329 CD denotes 2.5
T5779 330-332 NN denotes μg
T5781 332-333 SYM denotes /
T5782 333-335 NN denotes ml
T5780 336-345 NN denotes fungizone
T5783 346-347 -LRB- denotes (
T5785 347-357 NNP denotes Invitrogen
T5784 358-363 NNP denotes Corp.
T5786 363-364 -RRB- denotes )
T5787 364-366 , denotes ,
T5788 366-367 CD denotes 2
T5789 368-370 NN denotes μM
T5790 371-380 NN denotes forskolin
T5791 381-382 -LRB- denotes (
T5793 382-385 NNP denotes EMD
T5794 386-397 NNP denotes Biosciences
T5792 398-402 NNP denotes Inc.
T5795 402-403 -RRB- denotes )
T5796 404-407 CC denotes and
T5797 408-410 CD denotes 10
T5798 411-413 NN denotes ng
T5800 413-414 SYM denotes /
T5801 414-416 NN denotes ml
T5802 417-428 JJ denotes recombinant
T5803 429-438 NN denotes heregulin
T5804 438-439 HYPH denotes -
T5799 439-444 NN denotes beta1
T5805 445-446 -LRB- denotes (
T5807 446-447 NNP denotes R
T5808 447-448 CC denotes &
T5809 448-449 NNP denotes D
T5806 450-457 NNP denotes Systems
T5810 457-458 -RRB- denotes )
T5811 458-459 . denotes .
T5812 459-697 sentence denotes For dissociation, cultured sciatic nerves were cut into pieces and incubated at 37°C for 3 hours in Opti-MEM medium (Invitrogen Corp.) containing 130 U/ml collagenase type I (Invitrogen Corp.) and 0.4 U/ml dispase II (Roche Diagnostics).
T5813 460-463 IN denotes For
T5815 464-476 NN denotes dissociation
T5816 476-478 , denotes ,
T5817 478-486 VBN denotes cultured
T5819 487-494 JJ denotes sciatic
T5818 495-501 NNS denotes nerves
T5820 502-506 VBD denotes were
T5814 507-510 VBN denotes cut
T5821 511-515 IN denotes into
T5822 516-522 NNS denotes pieces
T5823 523-526 CC denotes and
T5824 527-536 VBN denotes incubated
T5825 537-539 IN denotes at
T5826 540-542 CD denotes 37
T5827 542-544 NN denotes °C
T5828 545-548 IN denotes for
T5829 549-550 CD denotes 3
T5830 551-556 NNS denotes hours
T5831 557-559 IN denotes in
T5832 560-564 NN denotes Opti
T5834 564-565 HYPH denotes -
T5833 565-568 NN denotes MEM
T5835 569-575 NN denotes medium
T5836 576-577 -LRB- denotes (
T5838 577-587 NNP denotes Invitrogen
T5837 588-593 NNP denotes Corp.
T5839 593-594 -RRB- denotes )
T5840 595-605 VBG denotes containing
T5841 606-609 CD denotes 130
T5842 610-611 NN denotes U
T5844 611-612 SYM denotes /
T5845 612-614 NN denotes ml
T5846 615-626 NN denotes collagenase
T5843 627-631 NN denotes type
T5847 632-633 CD denotes I
T5848 634-635 -LRB- denotes (
T5850 635-645 NNP denotes Invitrogen
T5849 646-651 NNP denotes Corp.
T5851 651-652 -RRB- denotes )
T5852 653-656 CC denotes and
T5853 657-660 CD denotes 0.4
T5854 661-662 NN denotes U
T5856 662-663 SYM denotes /
T5857 663-665 NN denotes ml
T5855 666-673 NN denotes dispase
T5858 674-676 CD denotes II
T5859 677-678 -LRB- denotes (
T5861 678-683 NNP denotes Roche
T5860 684-695 NNP denotes Diagnostics
T5862 695-696 -RRB- denotes )
T5863 696-697 . denotes .
T5864 697-827 sentence denotes Dissociated cells were resuspended in the pre-treatment medium and plated on Poly-D-Lysine/Laminin coated plate (BD Biosciences).
T5865 698-709 VBN denotes Dissociated
T5866 710-715 NNS denotes cells
T5868 716-720 VBD denotes were
T5867 721-732 VBN denotes resuspended
T5869 733-735 IN denotes in
T5870 736-739 DT denotes the
T5872 740-753 JJ denotes pre-treatment
T5871 754-760 NN denotes medium
T5873 761-764 CC denotes and
T5874 765-771 VBN denotes plated
T5875 772-774 IN denotes on
T5876 775-779 NN denotes Poly
T5878 779-780 HYPH denotes -
T5879 780-781 NN denotes D
T5880 781-782 HYPH denotes -
T5877 782-788 NN denotes Lysine
T5882 788-789 HYPH denotes /
T5883 789-796 NN denotes Laminin
T5881 797-803 VBN denotes coated
T5884 804-809 NN denotes plate
T5885 810-811 -LRB- denotes (
T5887 811-813 NN denotes BD
T5886 814-825 NNS denotes Biosciences
T5888 825-826 -RRB- denotes )
T5889 826-827 . denotes .
T5890 827-941 sentence denotes The purity of the cultured Schwann cells, as determined by indirect immunofluorescence analysis, approached 90 %.
T5891 828-831 DT denotes The
T5892 832-838 NN denotes purity
T5894 839-841 IN denotes of
T5895 842-845 DT denotes the
T5897 846-854 VBN denotes cultured
T5898 855-862 NNP denotes Schwann
T5896 863-868 NNS denotes cells
T5899 868-870 , denotes ,
T5900 870-872 IN denotes as
T5901 873-883 VBN denotes determined
T5902 884-886 IN denotes by
T5903 887-895 JJ denotes indirect
T5905 896-914 NN denotes immunofluorescence
T5904 915-923 NN denotes analysis
T5906 923-925 , denotes ,
T5893 925-935 VBD denotes approached
T5907 936-938 CD denotes 90
T5908 939-940 NN denotes %
T5909 940-941 . denotes .
R3560 T5708 T5709 amod Primary,cells
R3561 T5709 T5711 nsubjpass cells,prepared
R3562 T5710 T5709 compound Schwann,cells
R3563 T5712 T5711 auxpass were,prepared
R3564 T5713 T5711 prep from,prepared
R3565 T5714 T5715 nummod 4,month
R3566 T5715 T5717 npadvmod month,old
R3567 T5716 T5715 punct -,month
R3568 T5717 T5719 amod old,mice
R3569 T5718 T5717 punct -,old
R3570 T5719 T5713 pobj mice,from
R3571 T5720 T5719 nmod C57BL,mice
R3572 T5721 T5720 punct /,C57BL
R3573 T5722 T5720 nummod 6,C57BL
R3574 T5723 T5711 prep according,prepared
R3575 T5724 T5723 prep to,according
R3576 T5725 T5726 poss Manent,protocol
R3577 T5726 T5724 pobj protocol,to
R3578 T5727 T5725 case 's,Manent
R3579 T5728 T5729 punct [,32
R3580 T5729 T5726 parataxis 32,protocol
R3581 T5730 T5729 punct ],32
R3582 T5731 T5726 prep with,protocol
R3583 T5732 T5733 amod minor,modifications
R3584 T5733 T5731 pobj modifications,with
R3585 T5734 T5711 punct .,prepared
R3586 T5736 T5737 advmod Briefly,removed
R3587 T5738 T5737 punct ", ",removed
R3588 T5739 T5740 amod sciatic,nerves
R3589 T5740 T5737 nsubjpass nerves,removed
R3590 T5741 T5737 auxpass were,removed
R3591 T5742 T5737 cc and,removed
R3592 T5743 T5737 conj incubated,removed
R3593 T5744 T5743 prep for,incubated
R3594 T5745 T5746 nummod 7,days
R3595 T5746 T5744 pobj days,for
R3596 T5747 T5743 prep in,incubated
R3597 T5748 T5749 det the,medium
R3598 T5749 T5747 pobj medium,in
R3599 T5750 T5749 amod pre-treatment,medium
R3600 T5751 T5749 punct ", ",medium
R3601 T5752 T5753 dep which,consisted
R3602 T5753 T5749 relcl consisted,medium
R3603 T5754 T5753 prep of,consisted
R3604 T5755 T5756 compound D,MEM
R3605 T5756 T5754 pobj MEM,of
R3606 T5757 T5756 punct -,MEM
R3607 T5758 T5759 punct (,glucose
R3608 T5759 T5756 parataxis glucose,MEM
R3609 T5760 T5759 amod high,glucose
R3610 T5761 T5759 punct ),glucose
R3611 T5762 T5756 acl supplemented,MEM
R3612 T5763 T5762 prep with,supplemented
R3613 T5764 T5765 nummod 10,%
R3614 T5765 T5766 compound %,FCS
R3615 T5766 T5763 pobj FCS,with
R3616 T5767 T5766 punct ", ",FCS
R3617 T5768 T5769 nummod 50,mg
R3618 T5769 T5770 nmod mg,gentamicin
R3619 T5770 T5766 conj gentamicin,FCS
R3620 T5771 T5772 punct /,ml
R3621 T5772 T5769 prep ml,mg
R3622 T5773 T5774 punct (,Corp.
R3623 T5774 T5770 parataxis Corp.,gentamicin
R3624 T5775 T5774 compound Invitrogen,Corp.
R3625 T5776 T5774 punct ),Corp.
R3626 T5777 T5770 punct ", ",gentamicin
R3627 T5778 T5779 nummod 2.5,μg
R3628 T5779 T5780 nmod μg,fungizone
R3629 T5780 T5770 conj fungizone,gentamicin
R3630 T5781 T5782 punct /,ml
R3631 T5782 T5779 prep ml,μg
R3632 T5783 T5784 punct (,Corp.
R3633 T5784 T5780 parataxis Corp.,fungizone
R3634 T5785 T5784 compound Invitrogen,Corp.
R3635 T5786 T5784 punct ),Corp.
R3636 T5787 T5780 punct ", ",fungizone
R3637 T5788 T5789 nummod 2,μM
R3638 T5789 T5790 compound μM,forskolin
R3639 T5790 T5780 conj forskolin,fungizone
R3640 T5791 T5792 punct (,Inc.
R3641 T5792 T5790 parataxis Inc.,forskolin
R3642 T5793 T5792 compound EMD,Inc.
R3643 T5794 T5792 compound Biosciences,Inc.
R3644 T5795 T5792 punct ),Inc.
R3645 T5796 T5790 cc and,forskolin
R3646 T5797 T5798 nummod 10,ng
R3647 T5798 T5799 nmod ng,beta1
R3648 T5799 T5790 conj beta1,forskolin
R3649 T5800 T5801 punct /,ml
R3650 T5801 T5798 prep ml,ng
R3651 T5802 T5799 amod recombinant,beta1
R3652 T5803 T5799 compound heregulin,beta1
R3653 T5804 T5799 punct -,beta1
R3654 T5805 T5806 punct (,Systems
R3655 T5806 T5799 parataxis Systems,beta1
R3656 T5807 T5806 nmod R,Systems
R3657 T5808 T5807 cc &,R
R3658 T5809 T5807 conj D,R
R3659 T5810 T5806 punct ),Systems
R3660 T5811 T5737 punct .,removed
R3661 T5813 T5814 prep For,cut
R3662 T5815 T5813 pobj dissociation,For
R3663 T5816 T5814 punct ", ",cut
R3664 T5817 T5818 amod cultured,nerves
R3665 T5818 T5814 nsubjpass nerves,cut
R3666 T5819 T5818 amod sciatic,nerves
R3667 T5820 T5814 auxpass were,cut
R3668 T5821 T5814 prep into,cut
R3669 T5822 T5821 pobj pieces,into
R3670 T5823 T5814 cc and,cut
R3671 T5824 T5814 conj incubated,cut
R3672 T5825 T5824 prep at,incubated
R3673 T5826 T5827 nummod 37,°C
R3674 T5827 T5825 pobj °C,at
R3675 T5828 T5824 prep for,incubated
R3676 T5829 T5830 nummod 3,hours
R3677 T5830 T5828 pobj hours,for
R3678 T5831 T5824 prep in,incubated
R3679 T5832 T5833 compound Opti,MEM
R3680 T5833 T5835 compound MEM,medium
R3681 T5834 T5833 punct -,MEM
R3682 T5835 T5831 pobj medium,in
R3683 T5836 T5837 punct (,Corp.
R3684 T5837 T5835 parataxis Corp.,medium
R3685 T5838 T5837 compound Invitrogen,Corp.
R3686 T5839 T5837 punct ),Corp.
R3687 T5840 T5835 acl containing,medium
R3688 T5841 T5842 nummod 130,U
R3689 T5842 T5843 nmod U,type
R3690 T5843 T5840 dobj type,containing
R3691 T5844 T5845 punct /,ml
R3692 T5845 T5842 prep ml,U
R3693 T5846 T5843 compound collagenase,type
R3694 T5847 T5843 nummod I,type
R3695 T5848 T5849 punct (,Corp.
R3696 T5849 T5843 parataxis Corp.,type
R3697 T5850 T5849 compound Invitrogen,Corp.
R3698 T5851 T5849 punct ),Corp.
R3699 T5852 T5843 cc and,type
R3700 T5853 T5854 nummod 0.4,U
R3701 T5854 T5855 nmod U,dispase
R3702 T5855 T5843 conj dispase,type
R3703 T5856 T5857 punct /,ml
R3704 T5857 T5854 prep ml,U
R3705 T5858 T5855 nummod II,dispase
R3706 T5859 T5860 punct (,Diagnostics
R3707 T5860 T5855 parataxis Diagnostics,dispase
R3708 T5861 T5860 compound Roche,Diagnostics
R3709 T5862 T5860 punct ),Diagnostics
R3710 T5863 T5814 punct .,cut
R3711 T5865 T5866 amod Dissociated,cells
R3712 T5866 T5867 nsubjpass cells,resuspended
R3713 T5868 T5867 auxpass were,resuspended
R3714 T5869 T5867 prep in,resuspended
R3715 T5870 T5871 det the,medium
R3716 T5871 T5869 pobj medium,in
R3717 T5872 T5871 amod pre-treatment,medium
R3718 T5873 T5867 cc and,resuspended
R3719 T5874 T5867 conj plated,resuspended
R3720 T5875 T5874 prep on,plated
R3721 T5876 T5877 compound Poly,Lysine
R3722 T5877 T5881 npadvmod Lysine,coated
R3723 T5878 T5877 punct -,Lysine
R3724 T5879 T5877 compound D,Lysine
R3725 T5880 T5877 punct -,Lysine
R3726 T5881 T5884 amod coated,plate
R3727 T5882 T5877 punct /,Lysine
R3728 T5883 T5877 appos Laminin,Lysine
R3729 T5884 T5875 pobj plate,on
R3730 T5885 T5886 punct (,Biosciences
R3731 T5886 T5884 parataxis Biosciences,plate
R3732 T5887 T5886 compound BD,Biosciences
R3733 T5888 T5886 punct ),Biosciences
R3734 T5889 T5867 punct .,resuspended
R3735 T5891 T5892 det The,purity
R3736 T5892 T5893 nsubj purity,approached
R3737 T5894 T5892 prep of,purity
R3738 T5895 T5896 det the,cells
R3739 T5896 T5894 pobj cells,of
R3740 T5897 T5896 amod cultured,cells
R3741 T5898 T5896 compound Schwann,cells
R3742 T5899 T5892 punct ", ",purity
R3743 T5900 T5901 mark as,determined
R3744 T5901 T5892 advcl determined,purity
R3745 T5902 T5901 prep by,determined
R3746 T5903 T5904 amod indirect,analysis
R3747 T5904 T5902 pobj analysis,by
R3748 T5905 T5904 compound immunofluorescence,analysis
R3749 T5906 T5893 punct ", ",approached
R3750 T5907 T5908 nummod 90,%
R3751 T5908 T5893 dobj %,approached
R3752 T5909 T5893 punct .,approached