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PMC:1142324 / 16772-17701 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T4851 0-10 NN denotes Generation
T4852 11-13 IN denotes of
T4853 14-20 NN denotes Adam22
T4854 21-30 JJ denotes deficient
T4855 31-35 NNS denotes mice
T4856 35-127 sentence denotes The linearised targeting vector was electroporated into TT2 embryonic stem (ES) cells [31].
T4857 36-39 DT denotes The
T4859 40-50 VBN denotes linearised
T4860 51-60 NN denotes targeting
T4858 61-67 NN denotes vector
T4862 68-71 VBD denotes was
T4861 72-86 VBN denotes electroporated
T4863 87-91 IN denotes into
T4864 92-95 NN denotes TT2
T4866 96-105 JJ denotes embryonic
T4867 106-110 NN denotes stem
T4868 111-112 -LRB- denotes (
T4869 112-114 NN denotes ES
T4870 114-115 -RRB- denotes )
T4865 116-121 NNS denotes cells
T4871 122-123 -LRB- denotes [
T4872 123-125 CD denotes 31
T4873 125-126 -RRB- denotes ]
T4874 126-127 . denotes .
T4875 127-173 sentence denotes Homologous recombinants were selected by PCR.
T4876 128-138 JJ denotes Homologous
T4877 139-151 NNS denotes recombinants
T4879 152-156 VBD denotes were
T4878 157-165 VBN denotes selected
T4880 166-168 IN denotes by
T4881 169-172 NN denotes PCR
T4882 172-173 . denotes .
T4883 173-418 sentence denotes The extracted genomic DNA from each clone was amplified using the forward primer AGN2: 5'-GCCTGCTTGCCGAATATCATGGTGGAAAAT-3' in the PGKneo cassette, and the reverse primer MFP065R: 5'-ACTATTTCTGTGATGAGGGCACAGCATC-3' outside the targeting vector.
T4884 174-177 DT denotes The
T4886 178-187 VBN denotes extracted
T4887 188-195 JJ denotes genomic
T4885 196-199 NN denotes DNA
T4889 200-204 IN denotes from
T4890 205-209 DT denotes each
T4891 210-215 NN denotes clone
T4892 216-219 VBD denotes was
T4888 220-229 VBN denotes amplified
T4893 230-235 VBG denotes using
T4894 236-239 DT denotes the
T4896 240-247 JJ denotes forward
T4895 248-254 NN denotes primer
T4897 255-259 NN denotes AGN2
T4898 259-261 : denotes :
T4899 261-262 CD denotes 5
T4901 262-263 SYM denotes '
T4902 263-264 HYPH denotes -
T4900 264-294 NN denotes GCCTGCTTGCCGAATATCATGGTGGAAAAT
T4903 294-295 HYPH denotes -
T4904 295-296 CD denotes 3
T4905 296-297 SYM denotes '
T4906 298-300 IN denotes in
T4907 301-304 DT denotes the
T4909 305-311 NN denotes PGKneo
T4908 312-320 NN denotes cassette
T4910 320-322 , denotes ,
T4911 322-325 CC denotes and
T4912 326-329 DT denotes the
T4914 330-337 JJ denotes reverse
T4913 338-344 NN denotes primer
T4915 345-352 NN denotes MFP065R
T4916 352-354 : denotes :
T4917 354-355 CD denotes 5
T4919 355-356 SYM denotes '
T4920 356-357 HYPH denotes -
T4918 357-385 NN denotes ACTATTTCTGTGATGAGGGCACAGCATC
T4921 385-386 HYPH denotes -
T4922 386-387 CD denotes 3
T4923 387-388 SYM denotes '
T4924 389-396 IN denotes outside
T4925 397-400 DT denotes the
T4927 401-410 NN denotes targeting
T4926 411-417 NN denotes vector
T4928 417-418 . denotes .
T4929 418-548 sentence denotes Homologous recombined DNA was efficiently amplified by 35 cycles of the following amplification steps (94°C-30 s and 68°C-5 min).
T4930 419-429 JJ denotes Homologous
T4932 430-440 JJ denotes recombined
T4931 441-444 NN denotes DNA
T4934 445-448 VBD denotes was
T4935 449-460 RB denotes efficiently
T4933 461-470 VBN denotes amplified
T4936 471-473 IN denotes by
T4937 474-476 CD denotes 35
T4938 477-483 NNS denotes cycles
T4939 484-486 IN denotes of
T4940 487-490 DT denotes the
T4942 491-500 VBG denotes following
T4943 501-514 NN denotes amplification
T4941 515-520 NNS denotes steps
T4944 521-522 -LRB- denotes (
T4945 522-524 CD denotes 94
T4946 524-526 NN denotes °C
T4947 526-527 HYPH denotes -
T4948 527-529 CD denotes 30
T4949 530-531 NN denotes s
T4950 532-535 CC denotes and
T4951 536-538 CD denotes 68
T4952 538-540 NN denotes °C
T4953 540-541 HYPH denotes -
T4954 541-542 CD denotes 5
T4955 543-546 NN denotes min
T4956 546-547 -RRB- denotes )
T4957 547-548 . denotes .
T4958 548-605 sentence denotes The targeting efficiency of this construct was about 4%.
T4959 549-552 DT denotes The
T4961 553-562 NN denotes targeting
T4960 563-573 NN denotes efficiency
T4963 574-576 IN denotes of
T4964 577-581 DT denotes this
T4965 582-591 NN denotes construct
T4962 592-595 VBD denotes was
T4966 596-601 RB denotes about
T4967 602-603 CD denotes 4
T4968 603-604 NN denotes %
T4969 604-605 . denotes .
T4970 605-704 sentence denotes Correctly targeted ES clones were injected into fertilized ICR mouse eggs at the eight-cell stage.
T4971 606-615 RB denotes Correctly
T4972 616-624 VBN denotes targeted
T4974 625-627 NN denotes ES
T4973 628-634 NNS denotes clones
T4976 635-639 VBD denotes were
T4975 640-648 VBN denotes injected
T4977 649-653 IN denotes into
T4978 654-664 VBN denotes fertilized
T4980 665-668 NN denotes ICR
T4981 669-674 NN denotes mouse
T4979 675-679 NNS denotes eggs
T4982 680-682 IN denotes at
T4983 683-686 DT denotes the
T4985 687-692 CD denotes eight
T4987 692-693 HYPH denotes -
T4986 693-697 NN denotes cell
T4984 698-703 NN denotes stage
T4988 703-704 . denotes .
T4989 704-848 sentence denotes The resulting male chimeras were mated with C57BL/6N females, and heterozygous male and female mice were interbred to generate homozygous mice.
T4990 705-708 DT denotes The
T4992 709-718 VBG denotes resulting
T4993 719-723 JJ denotes male
T4991 724-732 NNS denotes chimeras
T4995 733-737 VBD denotes were
T4994 738-743 VBN denotes mated
T4996 744-748 IN denotes with
T4997 749-754 NN denotes C57BL
T4999 754-755 HYPH denotes /
T5000 755-757 CD denotes 6N
T4998 758-765 NNS denotes females
T5001 765-767 , denotes ,
T5002 767-770 CC denotes and
T5003 771-783 JJ denotes heterozygous
T5005 784-788 JJ denotes male
T5006 789-792 CC denotes and
T5007 793-799 JJ denotes female
T5004 800-804 NNS denotes mice
T5009 805-809 VBD denotes were
T5008 810-819 VBN denotes interbred
T5010 820-822 TO denotes to
T5011 823-831 VB denotes generate
T5012 832-842 JJ denotes homozygous
T5013 843-847 NNS denotes mice
T5014 847-848 . denotes .
T5015 848-929 sentence denotes The ataxic phenotypes of homozygous mice were observed in two independent lines.
T5016 849-852 DT denotes The
T5018 853-859 JJ denotes ataxic
T5017 860-870 NNS denotes phenotypes
T5020 871-873 IN denotes of
T5021 874-884 JJ denotes homozygous
T5022 885-889 NNS denotes mice
T5023 890-894 VBD denotes were
T5019 895-903 VBN denotes observed
T5024 904-906 IN denotes in
T5025 907-910 CD denotes two
T5027 911-922 JJ denotes independent
T5026 923-928 NNS denotes lines
T5028 928-929 . denotes .
R3019 T4852 T4851 prep of,Generation
R3020 T4853 T4854 npadvmod Adam22,deficient
R3021 T4854 T4855 amod deficient,mice
R3022 T4855 T4852 pobj mice,of
R3023 T4857 T4858 det The,vector
R3024 T4858 T4861 nsubjpass vector,electroporated
R3025 T4859 T4858 amod linearised,vector
R3026 T4860 T4858 compound targeting,vector
R3027 T4862 T4861 auxpass was,electroporated
R3028 T4863 T4861 prep into,electroporated
R3029 T4864 T4865 nmod TT2,cells
R3030 T4865 T4863 pobj cells,into
R3031 T4866 T4867 amod embryonic,stem
R3032 T4867 T4865 nmod stem,cells
R3033 T4868 T4867 punct (,stem
R3034 T4869 T4867 appos ES,stem
R3035 T4870 T4865 punct ),cells
R3036 T4871 T4872 punct [,31
R3037 T4872 T4861 parataxis 31,electroporated
R3038 T4873 T4872 punct ],31
R3039 T4874 T4861 punct .,electroporated
R3040 T4876 T4877 amod Homologous,recombinants
R3041 T4877 T4878 nsubjpass recombinants,selected
R3042 T4879 T4878 auxpass were,selected
R3043 T4880 T4878 prep by,selected
R3044 T4881 T4880 pobj PCR,by
R3045 T4882 T4878 punct .,selected
R3046 T4884 T4885 det The,DNA
R3047 T4885 T4888 nsubjpass DNA,amplified
R3048 T4886 T4885 amod extracted,DNA
R3049 T4887 T4885 amod genomic,DNA
R3050 T4889 T4885 prep from,DNA
R3051 T4890 T4891 det each,clone
R3052 T4891 T4889 pobj clone,from
R3053 T4892 T4888 auxpass was,amplified
R3054 T4893 T4888 advcl using,amplified
R3055 T4894 T4895 det the,primer
R3056 T4895 T4893 dobj primer,using
R3057 T4896 T4895 amod forward,primer
R3058 T4897 T4895 appos AGN2,primer
R3059 T4898 T4895 punct : ,primer
R3060 T4899 T4900 nummod 5,GCCTGCTTGCCGAATATCATGGTGGAAAAT
R3061 T4900 T4895 appos GCCTGCTTGCCGAATATCATGGTGGAAAAT,primer
R3062 T4901 T4899 punct ',5
R3063 T4902 T4900 punct -,GCCTGCTTGCCGAATATCATGGTGGAAAAT
R3064 T4903 T4900 punct -,GCCTGCTTGCCGAATATCATGGTGGAAAAT
R3065 T4904 T4900 nummod 3,GCCTGCTTGCCGAATATCATGGTGGAAAAT
R3066 T4905 T4900 punct ',GCCTGCTTGCCGAATATCATGGTGGAAAAT
R3067 T4906 T4895 prep in,primer
R3068 T4907 T4908 det the,cassette
R3069 T4908 T4906 pobj cassette,in
R3070 T4909 T4908 compound PGKneo,cassette
R3071 T4910 T4895 punct ", ",primer
R3072 T4911 T4895 cc and,primer
R3073 T4912 T4913 det the,primer
R3074 T4913 T4895 conj primer,primer
R3075 T4914 T4913 amod reverse,primer
R3076 T4915 T4913 appos MFP065R,primer
R3077 T4916 T4913 punct : ,primer
R3078 T4917 T4918 nummod 5,ACTATTTCTGTGATGAGGGCACAGCATC
R3079 T4918 T4913 appos ACTATTTCTGTGATGAGGGCACAGCATC,primer
R3080 T4919 T4917 punct ',5
R3081 T4920 T4918 punct -,ACTATTTCTGTGATGAGGGCACAGCATC
R3082 T4921 T4918 punct -,ACTATTTCTGTGATGAGGGCACAGCATC
R3083 T4922 T4918 nummod 3,ACTATTTCTGTGATGAGGGCACAGCATC
R3084 T4923 T4918 punct ',ACTATTTCTGTGATGAGGGCACAGCATC
R3085 T4924 T4913 prep outside,primer
R3086 T4925 T4926 det the,vector
R3087 T4926 T4924 pobj vector,outside
R3088 T4927 T4926 compound targeting,vector
R3089 T4928 T4888 punct .,amplified
R3090 T4930 T4931 amod Homologous,DNA
R3091 T4931 T4933 nsubjpass DNA,amplified
R3092 T4932 T4931 amod recombined,DNA
R3093 T4934 T4933 auxpass was,amplified
R3094 T4935 T4933 advmod efficiently,amplified
R3095 T4936 T4933 prep by,amplified
R3096 T4937 T4938 nummod 35,cycles
R3097 T4938 T4936 pobj cycles,by
R3098 T4939 T4938 prep of,cycles
R3099 T4940 T4941 det the,steps
R3100 T4941 T4939 pobj steps,of
R3101 T4942 T4941 amod following,steps
R3102 T4943 T4941 compound amplification,steps
R3103 T4944 T4941 punct (,steps
R3104 T4945 T4946 nummod 94,°C
R3105 T4946 T4941 appos °C,steps
R3106 T4947 T4946 punct -,°C
R3107 T4948 T4949 nummod 30,s
R3108 T4949 T4946 npadvmod s,°C
R3109 T4950 T4946 cc and,°C
R3110 T4951 T4952 nummod 68,°C
R3111 T4952 T4946 conj °C,°C
R3112 T4953 T4952 punct -,°C
R3113 T4954 T4955 nummod 5,min
R3114 T4955 T4952 npadvmod min,°C
R3115 T4956 T4933 punct ),amplified
R3116 T4957 T4933 punct .,amplified
R3117 T4959 T4960 det The,efficiency
R3118 T4960 T4962 nsubj efficiency,was
R3119 T4961 T4960 compound targeting,efficiency
R3120 T4963 T4960 prep of,efficiency
R3121 T4964 T4965 det this,construct
R3122 T4965 T4963 pobj construct,of
R3123 T4966 T4967 advmod about,4
R3124 T4967 T4968 nummod 4,%
R3125 T4968 T4962 attr %,was
R3126 T4969 T4962 punct .,was
R3127 T4971 T4972 advmod Correctly,targeted
R3128 T4972 T4973 amod targeted,clones
R3129 T4973 T4975 nsubjpass clones,injected
R3130 T4974 T4973 compound ES,clones
R3131 T4976 T4975 auxpass were,injected
R3132 T4977 T4975 prep into,injected
R3133 T4978 T4979 amod fertilized,eggs
R3134 T4979 T4977 pobj eggs,into
R3135 T4980 T4979 compound ICR,eggs
R3136 T4981 T4979 compound mouse,eggs
R3137 T4982 T4975 prep at,injected
R3138 T4983 T4984 det the,stage
R3139 T4984 T4982 pobj stage,at
R3140 T4985 T4986 nummod eight,cell
R3141 T4986 T4984 compound cell,stage
R3142 T4987 T4986 punct -,cell
R3143 T4988 T4975 punct .,injected
R3144 T4990 T4991 det The,chimeras
R3145 T4991 T4994 nsubjpass chimeras,mated
R3146 T4992 T4991 amod resulting,chimeras
R3147 T4993 T4991 amod male,chimeras
R3148 T4995 T4994 auxpass were,mated
R3149 T4996 T4994 prep with,mated
R3150 T4997 T4998 nmod C57BL,females
R3151 T4998 T4996 pobj females,with
R3152 T4999 T4997 punct /,C57BL
R3153 T5000 T4997 nummod 6N,C57BL
R3154 T5001 T4994 punct ", ",mated
R3155 T5002 T4994 cc and,mated
R3156 T5003 T5004 amod heterozygous,mice
R3157 T5004 T5008 nsubjpass mice,interbred
R3158 T5005 T5004 amod male,mice
R3159 T5006 T5005 cc and,male
R3160 T5007 T5005 conj female,male
R3161 T5008 T4994 conj interbred,mated
R3162 T5009 T5008 auxpass were,interbred
R3163 T5010 T5011 aux to,generate
R3164 T5011 T5008 advcl generate,interbred
R3165 T5012 T5013 amod homozygous,mice
R3166 T5013 T5011 dobj mice,generate
R3167 T5014 T5008 punct .,interbred
R3168 T5016 T5017 det The,phenotypes
R3169 T5017 T5019 nsubjpass phenotypes,observed
R3170 T5018 T5017 amod ataxic,phenotypes
R3171 T5020 T5017 prep of,phenotypes
R3172 T5021 T5022 amod homozygous,mice
R3173 T5022 T5020 pobj mice,of
R3174 T5023 T5019 auxpass were,observed
R3175 T5024 T5019 prep in,observed
R3176 T5025 T5026 nummod two,lines
R3177 T5026 T5024 pobj lines,in
R3178 T5027 T5026 amod independent,lines
R3179 T5028 T5019 punct .,observed