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PMC:1142324 / 16158-16770 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T4651 0-12 NN denotes Construction
T4652 13-15 IN denotes of
T4653 16-18 DT denotes an
T4655 19-25 NN denotes Adam22
T4656 26-30 NN denotes gene
T4658 30-31 HYPH denotes -
T4657 31-40 VBG denotes targeting
T4654 41-47 NN denotes vector
T4659 47-269 sentence denotes The 18-kb genomic DNA fragments covering exons 5, 6, 7, 8 and 9 of the Adam22 gene were amplified from C57BL/6 genomic DNA by PCR using Expand Hi-Fidelity enzyme mix (Roche Diagnostics) and primers designed for each exon.
T4660 48-51 DT denotes The
T4662 52-54 CD denotes 18
T4664 54-55 HYPH denotes -
T4663 55-57 NN denotes kb
T4665 58-65 JJ denotes genomic
T4666 66-69 NN denotes DNA
T4661 70-79 NNS denotes fragments
T4668 80-88 VBG denotes covering
T4669 89-94 NNS denotes exons
T4670 95-96 CD denotes 5
T4671 96-98 , denotes ,
T4672 98-99 CD denotes 6
T4673 99-101 , denotes ,
T4674 101-102 CD denotes 7
T4675 102-104 , denotes ,
T4676 104-105 CD denotes 8
T4677 106-109 CC denotes and
T4678 110-111 CD denotes 9
T4679 112-114 IN denotes of
T4680 115-118 DT denotes the
T4682 119-125 NN denotes Adam22
T4681 126-130 NN denotes gene
T4683 131-135 VBD denotes were
T4667 136-145 VBN denotes amplified
T4684 146-150 IN denotes from
T4685 151-156 NN denotes C57BL
T4687 156-157 HYPH denotes /
T4688 157-158 CD denotes 6
T4689 159-166 JJ denotes genomic
T4686 167-170 NN denotes DNA
T4690 171-173 IN denotes by
T4691 174-177 NN denotes PCR
T4692 178-183 VBG denotes using
T4693 184-190 NNP denotes Expand
T4695 191-193 NNP denotes Hi
T4697 193-194 HYPH denotes -
T4696 194-202 NNP denotes Fidelity
T4698 203-209 NN denotes enzyme
T4694 210-213 NN denotes mix
T4699 214-215 -LRB- denotes (
T4701 215-220 NNP denotes Roche
T4700 221-232 NNP denotes Diagnostics
T4702 232-233 -RRB- denotes )
T4703 234-237 CC denotes and
T4704 238-245 NNS denotes primers
T4705 246-254 VBN denotes designed
T4706 255-258 IN denotes for
T4707 259-263 DT denotes each
T4708 264-268 NN denotes exon
T4709 268-269 . denotes .
T4710 269-382 sentence denotes To generate a mutation in the mouse Adam22 gene, we inserted the PGKneo cassette into exon 8 of the Adam22 gene.
T4711 270-272 TO denotes To
T4712 273-281 VB denotes generate
T4714 282-283 DT denotes a
T4715 284-292 NN denotes mutation
T4716 293-295 IN denotes in
T4717 296-299 DT denotes the
T4719 300-305 NN denotes mouse
T4720 306-312 NN denotes Adam22
T4718 313-317 NN denotes gene
T4721 317-319 , denotes ,
T4722 319-321 PRP denotes we
T4713 322-330 VBD denotes inserted
T4723 331-334 DT denotes the
T4725 335-341 NN denotes PGKneo
T4724 342-350 NN denotes cassette
T4726 351-355 IN denotes into
T4727 356-360 NN denotes exon
T4728 361-362 CD denotes 8
T4729 363-365 IN denotes of
T4730 366-369 DT denotes the
T4732 370-376 NN denotes Adam22
T4731 377-381 NN denotes gene
T4733 381-382 . denotes .
T4734 382-612 sentence denotes The final targeting construct consisted of a 10.5-kb genomic DNA fragment that was interrupted by the insertion of the PGKneo cassette and contained a MC1/TK cassette as a negative selection marker against random integration [5].
T4735 383-386 DT denotes The
T4737 387-392 JJ denotes final
T4738 393-402 NN denotes targeting
T4736 403-412 NN denotes construct
T4739 413-422 VBD denotes consisted
T4740 423-425 IN denotes of
T4741 426-427 DT denotes a
T4743 428-432 CD denotes 10.5
T4745 432-433 HYPH denotes -
T4744 433-435 NN denotes kb
T4746 436-443 JJ denotes genomic
T4747 444-447 NN denotes DNA
T4742 448-456 NN denotes fragment
T4748 457-461 WDT denotes that
T4750 462-465 VBD denotes was
T4749 466-477 VBN denotes interrupted
T4751 478-480 IN denotes by
T4752 481-484 DT denotes the
T4753 485-494 NN denotes insertion
T4754 495-497 IN denotes of
T4755 498-501 DT denotes the
T4757 502-508 NN denotes PGKneo
T4756 509-517 NN denotes cassette
T4758 518-521 CC denotes and
T4759 522-531 VBD denotes contained
T4760 532-533 DT denotes a
T4762 534-537 NN denotes MC1
T4764 537-538 HYPH denotes /
T4763 538-540 NN denotes TK
T4761 541-549 NN denotes cassette
T4765 550-552 IN denotes as
T4766 553-554 DT denotes a
T4768 555-563 JJ denotes negative
T4769 564-573 NN denotes selection
T4767 574-580 NN denotes marker
T4770 581-588 IN denotes against
T4771 589-595 JJ denotes random
T4772 596-607 NN denotes integration
T4773 608-609 -LRB- denotes [
T4774 609-610 CD denotes 5
T4775 610-611 -RRB- denotes ]
T4776 611-612 . denotes .
R2896 T4652 T4651 prep of,Construction
R2897 T4653 T4654 det an,vector
R2898 T4654 T4652 pobj vector,of
R2899 T4655 T4654 nmod Adam22,vector
R2900 T4656 T4657 npadvmod gene,targeting
R2901 T4657 T4654 amod targeting,vector
R2902 T4658 T4657 punct -,targeting
R2903 T4660 T4661 det The,fragments
R2904 T4661 T4667 nsubjpass fragments,amplified
R2905 T4662 T4663 nummod 18,kb
R2906 T4663 T4661 nmod kb,fragments
R2907 T4664 T4663 punct -,kb
R2908 T4665 T4661 amod genomic,fragments
R2909 T4666 T4661 compound DNA,fragments
R2910 T4668 T4661 acl covering,fragments
R2911 T4669 T4670 nmod exons,5
R2912 T4670 T4668 dobj 5,covering
R2913 T4671 T4670 punct ", ",5
R2914 T4672 T4670 conj 6,5
R2915 T4673 T4672 punct ", ",6
R2916 T4674 T4672 conj 7,6
R2917 T4675 T4674 punct ", ",7
R2918 T4676 T4674 conj 8,7
R2919 T4677 T4676 cc and,8
R2920 T4678 T4676 conj 9,8
R2921 T4679 T4670 prep of,5
R2922 T4680 T4681 det the,gene
R2923 T4681 T4679 pobj gene,of
R2924 T4682 T4681 compound Adam22,gene
R2925 T4683 T4667 auxpass were,amplified
R2926 T4684 T4667 prep from,amplified
R2927 T4685 T4686 nmod C57BL,DNA
R2928 T4686 T4684 pobj DNA,from
R2929 T4687 T4685 punct /,C57BL
R2930 T4688 T4685 nummod 6,C57BL
R2931 T4689 T4686 amod genomic,DNA
R2932 T4690 T4667 prep by,amplified
R2933 T4691 T4690 pobj PCR,by
R2934 T4692 T4667 advcl using,amplified
R2935 T4693 T4694 compound Expand,mix
R2936 T4694 T4692 dobj mix,using
R2937 T4695 T4696 compound Hi,Fidelity
R2938 T4696 T4694 compound Fidelity,mix
R2939 T4697 T4696 punct -,Fidelity
R2940 T4698 T4694 compound enzyme,mix
R2941 T4699 T4700 punct (,Diagnostics
R2942 T4700 T4694 parataxis Diagnostics,mix
R2943 T4701 T4700 compound Roche,Diagnostics
R2944 T4702 T4700 punct ),Diagnostics
R2945 T4703 T4694 cc and,mix
R2946 T4704 T4694 conj primers,mix
R2947 T4705 T4704 acl designed,primers
R2948 T4706 T4705 prep for,designed
R2949 T4707 T4708 det each,exon
R2950 T4708 T4706 pobj exon,for
R2951 T4709 T4667 punct .,amplified
R2952 T4711 T4712 aux To,generate
R2953 T4712 T4713 advcl generate,inserted
R2954 T4714 T4715 det a,mutation
R2955 T4715 T4712 dobj mutation,generate
R2956 T4716 T4712 prep in,generate
R2957 T4717 T4718 det the,gene
R2958 T4718 T4716 pobj gene,in
R2959 T4719 T4718 compound mouse,gene
R2960 T4720 T4718 compound Adam22,gene
R2961 T4721 T4713 punct ", ",inserted
R2962 T4722 T4713 nsubj we,inserted
R2963 T4723 T4724 det the,cassette
R2964 T4724 T4713 dobj cassette,inserted
R2965 T4725 T4724 compound PGKneo,cassette
R2966 T4726 T4713 prep into,inserted
R2967 T4727 T4726 pobj exon,into
R2968 T4728 T4727 nummod 8,exon
R2969 T4729 T4727 prep of,exon
R2970 T4730 T4731 det the,gene
R2971 T4731 T4729 pobj gene,of
R2972 T4732 T4731 compound Adam22,gene
R2973 T4733 T4713 punct .,inserted
R2974 T4735 T4736 det The,construct
R2975 T4736 T4739 nsubj construct,consisted
R2976 T4737 T4736 amod final,construct
R2977 T4738 T4736 compound targeting,construct
R2978 T4740 T4739 prep of,consisted
R2979 T4741 T4742 det a,fragment
R2980 T4742 T4740 pobj fragment,of
R2981 T4743 T4744 nummod 10.5,kb
R2982 T4744 T4742 nmod kb,fragment
R2983 T4745 T4744 punct -,kb
R2984 T4746 T4747 amod genomic,DNA
R2985 T4747 T4742 compound DNA,fragment
R2986 T4748 T4749 dep that,interrupted
R2987 T4749 T4742 relcl interrupted,fragment
R2988 T4750 T4749 auxpass was,interrupted
R2989 T4751 T4749 agent by,interrupted
R2990 T4752 T4753 det the,insertion
R2991 T4753 T4751 pobj insertion,by
R2992 T4754 T4753 prep of,insertion
R2993 T4755 T4756 det the,cassette
R2994 T4756 T4754 pobj cassette,of
R2995 T4757 T4756 compound PGKneo,cassette
R2996 T4758 T4749 cc and,interrupted
R2997 T4759 T4749 conj contained,interrupted
R2998 T4760 T4761 det a,cassette
R2999 T4761 T4759 dobj cassette,contained
R3000 T4762 T4763 compound MC1,TK
R3001 T4763 T4761 compound TK,cassette
R3002 T4764 T4763 punct /,TK
R3003 T4765 T4759 prep as,contained
R3004 T4766 T4767 det a,marker
R3005 T4767 T4765 pobj marker,as
R3006 T4768 T4767 amod negative,marker
R3007 T4769 T4767 compound selection,marker
R3008 T4770 T4767 prep against,marker
R3009 T4771 T4772 amod random,integration
R3010 T4772 T4770 pobj integration,against
R3011 T4773 T4774 punct [,5
R3012 T4774 T4739 parataxis 5,consisted
R3013 T4775 T4774 punct ],5
R3014 T4776 T4739 punct .,consisted