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PMC:1140370 / 9612-10893 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T2967 0-8 NN denotes Nuclease
T2968 9-19 NN denotes protection
T2969 20-26 NNS denotes assays
T2970 26-278 sentence denotes The reaction mixtures (25 μl) contained 25 mM HEPES–NaOH (pH 7.5), 50 mM sodium acetate, 10 mM Mg(CH3COO)2, 1 mM DTT, 0.25 mg/ml BSA, 0.5 mM ATP-γ-S, 0.5 mM CaCl2, 10 fmol of 32P-labeled bubble substrate, and the indicated amount of Mcm4/6/7 proteins.
T2971 27-30 DT denotes The
T2973 31-39 NN denotes reaction
T2972 40-48 NNS denotes mixtures
T2975 49-50 -LRB- denotes (
T2977 50-52 CD denotes 25
T2976 53-55 NN denotes μl
T2978 55-56 -RRB- denotes )
T2974 57-66 VBD denotes contained
T2979 67-69 CD denotes 25
T2980 70-72 NN denotes mM
T2982 73-78 NN denotes HEPES
T2983 78-79 HYPH denotes –
T2981 79-83 NN denotes NaOH
T2984 84-85 -LRB- denotes (
T2985 85-87 NN denotes pH
T2986 88-91 CD denotes 7.5
T2987 91-92 -RRB- denotes )
T2988 92-94 , denotes ,
T2989 94-96 CD denotes 50
T2990 97-99 NN denotes mM
T2992 100-106 NN denotes sodium
T2991 107-114 NN denotes acetate
T2993 114-116 , denotes ,
T2994 116-118 CD denotes 10
T2995 119-121 NN denotes mM
T2997 122-124 NN denotes Mg
T2998 124-125 -LRB- denotes (
T2996 125-131 NN denotes CH3COO
T2999 131-132 -RRB- denotes )
T3000 132-133 CD denotes 2
T3001 133-135 , denotes ,
T3002 135-136 CD denotes 1
T3003 137-139 NN denotes mM
T3004 140-143 NN denotes DTT
T3005 143-145 , denotes ,
T3006 145-149 CD denotes 0.25
T3007 150-152 NN denotes mg
T3009 152-153 SYM denotes /
T3010 153-155 NN denotes ml
T3008 156-159 NN denotes BSA
T3011 159-161 , denotes ,
T3012 161-164 CD denotes 0.5
T3013 165-167 NN denotes mM
T3015 168-171 NN denotes ATP
T3016 171-172 HYPH denotes -
T3017 172-173 SYM denotes γ
T3018 173-174 HYPH denotes -
T3014 174-175 NN denotes S
T3019 175-177 , denotes ,
T3020 177-180 CD denotes 0.5
T3021 181-183 NN denotes mM
T3022 184-189 NN denotes CaCl2
T3023 189-191 , denotes ,
T3024 191-193 CD denotes 10
T3025 194-198 NN denotes fmol
T3026 199-201 IN denotes of
T3027 202-205 NN denotes 32P
T3029 205-206 HYPH denotes -
T3028 206-213 VBN denotes labeled
T3031 214-220 NN denotes bubble
T3030 221-230 NN denotes substrate
T3032 230-232 , denotes ,
T3033 232-235 CC denotes and
T3034 236-239 DT denotes the
T3036 240-249 VBN denotes indicated
T3035 250-256 NN denotes amount
T3037 257-259 IN denotes of
T3038 260-264 NN denotes Mcm4
T3040 264-265 HYPH denotes /
T3041 265-266 CD denotes 6
T3042 266-267 HYPH denotes /
T3043 267-268 CD denotes 7
T3039 269-277 NN denotes proteins
T3044 277-278 . denotes .
T3045 278-448 sentence denotes After incubation at 30°C for 30 min, the indicated amount of DNase I (TAKARA Biomedical, Japan) was added, and the mixtures were incubated at room temperature for 1 min.
T3046 279-284 IN denotes After
T3048 285-295 NN denotes incubation
T3049 296-298 IN denotes at
T3050 299-301 CD denotes 30
T3051 301-303 NN denotes °C
T3052 304-307 IN denotes for
T3053 308-310 CD denotes 30
T3054 311-314 NN denotes min
T3055 314-316 , denotes ,
T3056 316-319 DT denotes the
T3058 320-329 VBN denotes indicated
T3057 330-336 NN denotes amount
T3059 337-339 IN denotes of
T3060 340-345 NN denotes DNase
T3061 346-347 CD denotes I
T3062 348-349 -LRB- denotes (
T3064 349-355 NNP denotes TAKARA
T3063 356-366 NNP denotes Biomedical
T3065 366-368 , denotes ,
T3066 368-373 NNP denotes Japan
T3067 373-374 -RRB- denotes )
T3068 375-378 VBD denotes was
T3047 379-384 VBN denotes added
T3069 384-386 , denotes ,
T3070 386-389 CC denotes and
T3071 390-393 DT denotes the
T3072 394-402 NNS denotes mixtures
T3074 403-407 VBD denotes were
T3073 408-417 VBN denotes incubated
T3075 418-420 IN denotes at
T3076 421-425 NN denotes room
T3077 426-437 NN denotes temperature
T3078 438-441 IN denotes for
T3079 442-443 CD denotes 1
T3080 444-447 NN denotes min
T3081 447-448 . denotes .
T3082 448-584 sentence denotes DNase I was inactivated by the addition of 250 μl of stop solution (20 mM EDTA, 0.2M sodium chloride, 1% SDS and 12.5 μg/ml yeast RNA).
T3083 449-454 NN denotes DNase
T3085 455-456 CD denotes I
T3086 457-460 VBD denotes was
T3084 461-472 VBN denotes inactivated
T3087 473-475 IN denotes by
T3088 476-479 DT denotes the
T3089 480-488 NN denotes addition
T3090 489-491 IN denotes of
T3091 492-495 CD denotes 250
T3092 496-498 NN denotes μl
T3093 499-501 IN denotes of
T3094 502-506 NN denotes stop
T3095 507-515 NN denotes solution
T3096 516-517 -LRB- denotes (
T3098 517-519 CD denotes 20
T3099 520-522 NN denotes mM
T3097 523-527 NN denotes EDTA
T3100 527-529 , denotes ,
T3101 529-533 NN denotes 0.2M
T3103 534-540 NN denotes sodium
T3102 541-549 NN denotes chloride
T3104 549-551 , denotes ,
T3105 551-552 CD denotes 1
T3106 552-553 NN denotes %
T3107 554-557 NN denotes SDS
T3108 558-561 CC denotes and
T3109 562-566 CD denotes 12.5
T3110 567-569 NN denotes μg
T3112 569-570 SYM denotes /
T3113 570-572 NN denotes ml
T3114 573-578 NN denotes yeast
T3111 579-582 NN denotes RNA
T3115 582-583 -RRB- denotes )
T3116 583-584 . denotes .
T3117 584-712 sentence denotes For nuclease P1 footprinting assay, binding reactions were carried out under the same conditions except that CaCl2 was omitted.
T3118 585-588 IN denotes For
T3120 589-597 NN denotes nuclease
T3122 598-600 NN denotes P1
T3121 601-613 NN denotes footprinting
T3123 614-619 NN denotes assay
T3124 619-621 , denotes ,
T3125 621-628 NN denotes binding
T3126 629-638 NNS denotes reactions
T3127 639-643 VBD denotes were
T3119 644-651 VBN denotes carried
T3128 652-655 RP denotes out
T3129 656-661 IN denotes under
T3130 662-665 DT denotes the
T3132 666-670 JJ denotes same
T3131 671-681 NNS denotes conditions
T3133 682-688 IN denotes except
T3134 689-693 IN denotes that
T3136 694-699 NN denotes CaCl2
T3137 700-703 VBD denotes was
T3135 704-711 VBN denotes omitted
T3138 711-712 . denotes .
T3139 712-797 sentence denotes P1 nuclease (0.3 U, Roche) was added and incubation was continued at 37°C for 1 min.
T3140 713-715 NN denotes P1
T3141 716-724 NN denotes nuclease
T3143 725-726 -LRB- denotes (
T3145 726-729 CD denotes 0.3
T3146 730-731 NNP denotes U
T3147 731-733 , denotes ,
T3144 733-738 NNP denotes Roche
T3148 738-739 -RRB- denotes )
T3149 740-743 VBD denotes was
T3142 744-749 VBN denotes added
T3150 750-753 CC denotes and
T3151 754-764 NN denotes incubation
T3153 765-768 VBD denotes was
T3152 769-778 VBN denotes continued
T3154 779-781 IN denotes at
T3155 782-784 CD denotes 37
T3156 784-786 NN denotes °C
T3157 787-790 IN denotes for
T3158 791-792 CD denotes 1
T3159 793-796 NN denotes min
T3160 796-797 . denotes .
T3161 797-992 sentence denotes Cleavage was stopped by addition of 50 μl of 25 mM EDTA and 0.4 M NaOH; the mixture was incubated at room temperature for 10 min followed by the addition of 12 μl of 3 M sodium acetate (pH 4.8).
T3162 798-806 NN denotes Cleavage
T3164 807-810 VBD denotes was
T3163 811-818 VBN denotes stopped
T3166 819-821 IN denotes by
T3167 822-830 NN denotes addition
T3168 831-833 IN denotes of
T3169 834-836 CD denotes 50
T3170 837-839 NN denotes μl
T3171 840-842 IN denotes of
T3172 843-845 CD denotes 25
T3173 846-848 NN denotes mM
T3174 849-853 NN denotes EDTA
T3175 854-857 CC denotes and
T3176 858-861 CD denotes 0.4
T3177 862-863 NN denotes M
T3178 864-868 NN denotes NaOH
T3179 868-869 : denotes ;
T3180 870-873 DT denotes the
T3181 874-881 NN denotes mixture
T3182 882-885 VBD denotes was
T3165 886-895 VBN denotes incubated
T3183 896-898 IN denotes at
T3184 899-903 NN denotes room
T3185 904-915 NN denotes temperature
T3186 916-919 IN denotes for
T3187 920-922 CD denotes 10
T3188 923-926 NN denotes min
T3189 927-935 VBN denotes followed
T3190 936-938 IN denotes by
T3191 939-942 DT denotes the
T3192 943-951 NN denotes addition
T3193 952-954 IN denotes of
T3194 955-957 CD denotes 12
T3195 958-960 NN denotes μl
T3196 961-963 IN denotes of
T3197 964-965 CD denotes 3
T3198 966-967 NN denotes M
T3200 968-974 NN denotes sodium
T3199 975-982 NN denotes acetate
T3201 983-984 -LRB- denotes (
T3202 984-986 NN denotes pH
T3203 987-990 CD denotes 4.8
T3204 990-991 -RRB- denotes )
T3205 991-992 . denotes .
T3206 992-1131 sentence denotes Proteins were removed by phenol–chloroform extraction, and DNAs were collected by ethanol precipitation followed by wash with 70% ethanol.
T3207 993-1001 NNS denotes Proteins
T3209 1002-1006 VBD denotes were
T3208 1007-1014 VBN denotes removed
T3210 1015-1017 IN denotes by
T3211 1018-1024 NN denotes phenol
T3213 1024-1025 HYPH denotes –
T3212 1025-1035 NN denotes chloroform
T3214 1036-1046 NN denotes extraction
T3215 1046-1048 , denotes ,
T3216 1048-1051 CC denotes and
T3217 1052-1056 NNS denotes DNAs
T3219 1057-1061 VBD denotes were
T3218 1062-1071 VBN denotes collected
T3220 1072-1074 IN denotes by
T3221 1075-1082 NN denotes ethanol
T3222 1083-1096 NN denotes precipitation
T3223 1097-1105 VBN denotes followed
T3224 1106-1108 IN denotes by
T3225 1109-1113 NN denotes wash
T3226 1114-1118 IN denotes with
T3227 1119-1121 CD denotes 70
T3228 1121-1122 NN denotes %
T3229 1123-1130 NN denotes ethanol
T3230 1130-1131 . denotes .
T3231 1131-1281 sentence denotes The digested DNA was subjected to electrophoresis through a 10 or 12% polyacrylamide sequencing gel containing 7 M urea, followed by autoradiography.
T3232 1132-1135 DT denotes The
T3234 1136-1144 VBN denotes digested
T3233 1145-1148 NN denotes DNA
T3236 1149-1152 VBD denotes was
T3235 1153-1162 VBN denotes subjected
T3237 1163-1165 IN denotes to
T3238 1166-1181 NN denotes electrophoresis
T3239 1182-1189 IN denotes through
T3240 1190-1191 DT denotes a
T3242 1192-1194 CD denotes 10
T3244 1195-1197 CC denotes or
T3245 1198-1200 CD denotes 12
T3243 1200-1201 NN denotes %
T3246 1202-1216 NN denotes polyacrylamide
T3247 1217-1227 NN denotes sequencing
T3241 1228-1231 NN denotes gel
T3248 1232-1242 VBG denotes containing
T3249 1243-1244 CD denotes 7
T3250 1245-1246 NN denotes M
T3251 1247-1251 NN denotes urea
T3252 1251-1253 , denotes ,
T3253 1253-1261 VBN denotes followed
T3254 1262-1264 IN denotes by
T3255 1265-1280 NN denotes autoradiography
T3256 1280-1281 . denotes .
R1880 T2967 T2968 compound Nuclease,protection
R1881 T2968 T2969 compound protection,assays
R1882 T2971 T2972 det The,mixtures
R1883 T2972 T2974 nsubj mixtures,contained
R1884 T2973 T2972 compound reaction,mixtures
R1885 T2975 T2976 punct (,μl
R1886 T2976 T2972 parataxis μl,mixtures
R1887 T2977 T2976 nummod 25,μl
R1888 T2978 T2976 punct ),μl
R1889 T2979 T2980 nummod 25,mM
R1890 T2980 T2981 compound mM,NaOH
R1891 T2981 T2974 dobj NaOH,contained
R1892 T2982 T2981 compound HEPES,NaOH
R1893 T2983 T2981 punct –,NaOH
R1894 T2984 T2985 punct (,pH
R1895 T2985 T2981 parataxis pH,NaOH
R1896 T2986 T2985 nummod 7.5,pH
R1897 T2987 T2985 punct ),pH
R1898 T2988 T2981 punct ", ",NaOH
R1899 T2989 T2990 nummod 50,mM
R1900 T2990 T2991 compound mM,acetate
R1901 T2991 T2981 conj acetate,NaOH
R1902 T2992 T2991 compound sodium,acetate
R1903 T2993 T2991 punct ", ",acetate
R1904 T2994 T2995 nummod 10,mM
R1905 T2995 T2996 nmod mM,CH3COO
R1906 T2996 T2991 conj CH3COO,acetate
R1907 T2997 T2996 nmod Mg,CH3COO
R1908 T2998 T2996 punct (,CH3COO
R1909 T2999 T2996 punct ),CH3COO
R1910 T3000 T2996 nummod 2,CH3COO
R1911 T3001 T2996 punct ", ",CH3COO
R1912 T3002 T3003 nummod 1,mM
R1913 T3003 T3004 compound mM,DTT
R1914 T3004 T2996 conj DTT,CH3COO
R1915 T3005 T3004 punct ", ",DTT
R1916 T3006 T3007 nummod 0.25,mg
R1917 T3007 T3008 nmod mg,BSA
R1918 T3008 T3004 conj BSA,DTT
R1919 T3009 T3010 punct /,ml
R1920 T3010 T3007 prep ml,mg
R1921 T3011 T3008 punct ", ",BSA
R1922 T3012 T3013 nummod 0.5,mM
R1923 T3013 T3014 nmod mM,S
R1924 T3014 T3008 conj S,BSA
R1925 T3015 T3014 nmod ATP,S
R1926 T3016 T3014 punct -,S
R1927 T3017 T3014 punct γ,S
R1928 T3018 T3014 punct -,S
R1929 T3019 T3014 punct ", ",S
R1930 T3020 T3021 nummod 0.5,mM
R1931 T3021 T3022 compound mM,CaCl2
R1932 T3022 T3014 conj CaCl2,S
R1933 T3023 T3022 punct ", ",CaCl2
R1934 T3024 T3025 nummod 10,fmol
R1935 T3025 T3022 conj fmol,CaCl2
R1936 T3026 T3025 prep of,fmol
R1937 T3027 T3028 npadvmod 32P,labeled
R1938 T3028 T3030 amod labeled,substrate
R1939 T3029 T3028 punct -,labeled
R1940 T3030 T3026 pobj substrate,of
R1941 T3031 T3030 compound bubble,substrate
R1942 T3032 T3025 punct ", ",fmol
R1943 T3033 T3025 cc and,fmol
R1944 T3034 T3035 det the,amount
R1945 T3035 T3025 conj amount,fmol
R1946 T3036 T3035 amod indicated,amount
R1947 T3037 T3035 prep of,amount
R1948 T3038 T3039 nmod Mcm4,proteins
R1949 T3039 T3037 pobj proteins,of
R1950 T3040 T3038 punct /,Mcm4
R1951 T3041 T3038 nummod 6,Mcm4
R1952 T3042 T3038 punct /,Mcm4
R1953 T3043 T3038 nummod 7,Mcm4
R1954 T3044 T2974 punct .,contained
R1955 T3046 T3047 prep After,added
R1956 T3048 T3046 pobj incubation,After
R1957 T3049 T3048 prep at,incubation
R1958 T3050 T3051 nummod 30,°C
R1959 T3051 T3049 pobj °C,at
R1960 T3052 T3048 prep for,incubation
R1961 T3053 T3054 nummod 30,min
R1962 T3054 T3052 pobj min,for
R1963 T3055 T3047 punct ", ",added
R1964 T3056 T3057 det the,amount
R1965 T3057 T3047 nsubjpass amount,added
R1966 T3058 T3057 amod indicated,amount
R1967 T3059 T3057 prep of,amount
R1968 T3060 T3059 pobj DNase,of
R1969 T3061 T3060 nummod I,DNase
R1970 T3062 T3063 punct (,Biomedical
R1971 T3063 T3060 parataxis Biomedical,DNase
R1972 T3064 T3063 compound TAKARA,Biomedical
R1973 T3065 T3063 punct ", ",Biomedical
R1974 T3066 T3063 npadvmod Japan,Biomedical
R1975 T3067 T3063 punct ),Biomedical
R1976 T3068 T3047 auxpass was,added
R1977 T3069 T3047 punct ", ",added
R1978 T3070 T3047 cc and,added
R1979 T3071 T3072 det the,mixtures
R1980 T3072 T3073 nsubjpass mixtures,incubated
R1981 T3073 T3047 conj incubated,added
R1982 T3074 T3073 auxpass were,incubated
R1983 T3075 T3073 prep at,incubated
R1984 T3076 T3077 compound room,temperature
R1985 T3077 T3075 pobj temperature,at
R1986 T3078 T3073 prep for,incubated
R1987 T3079 T3080 nummod 1,min
R1988 T3080 T3078 pobj min,for
R1989 T3081 T3047 punct .,added
R1990 T3083 T3084 nsubjpass DNase,inactivated
R1991 T3085 T3083 nummod I,DNase
R1992 T3086 T3084 auxpass was,inactivated
R1993 T3087 T3084 agent by,inactivated
R1994 T3088 T3089 det the,addition
R1995 T3089 T3087 pobj addition,by
R1996 T3090 T3089 prep of,addition
R1997 T3091 T3092 nummod 250,μl
R1998 T3092 T3090 pobj μl,of
R1999 T3093 T3092 prep of,μl
R2000 T3094 T3095 compound stop,solution
R2001 T3095 T3093 pobj solution,of
R2002 T3096 T3097 punct (,EDTA
R2003 T3097 T3084 parataxis EDTA,inactivated
R2004 T3098 T3099 nummod 20,mM
R2005 T3099 T3097 compound mM,EDTA
R2006 T3100 T3097 punct ", ",EDTA
R2007 T3101 T3102 compound 0.2M,chloride
R2008 T3102 T3097 conj chloride,EDTA
R2009 T3103 T3102 compound sodium,chloride
R2010 T3104 T3102 punct ", ",chloride
R2011 T3105 T3106 nummod 1,%
R2012 T3106 T3107 compound %,SDS
R2013 T3107 T3102 conj SDS,chloride
R2014 T3108 T3107 cc and,SDS
R2015 T3109 T3110 nummod 12.5,μg
R2016 T3110 T3111 nmod μg,RNA
R2017 T3111 T3107 conj RNA,SDS
R2018 T3112 T3113 punct /,ml
R2019 T3113 T3110 prep ml,μg
R2020 T3114 T3111 compound yeast,RNA
R2021 T3115 T3111 punct ),RNA
R2022 T3116 T3084 punct .,inactivated
R2023 T3118 T3119 prep For,carried
R2024 T3120 T3121 compound nuclease,footprinting
R2025 T3121 T3123 compound footprinting,assay
R2026 T3122 T3121 compound P1,footprinting
R2027 T3123 T3118 pobj assay,For
R2028 T3124 T3119 punct ", ",carried
R2029 T3125 T3126 compound binding,reactions
R2030 T3126 T3119 nsubjpass reactions,carried
R2031 T3127 T3119 auxpass were,carried
R2032 T3128 T3119 prt out,carried
R2033 T3129 T3119 prep under,carried
R2034 T3130 T3131 det the,conditions
R2035 T3131 T3129 pobj conditions,under
R2036 T3132 T3131 amod same,conditions
R2037 T3133 T3131 prep except,conditions
R2038 T3134 T3135 mark that,omitted
R2039 T3135 T3133 pcomp omitted,except
R2040 T3136 T3135 nsubjpass CaCl2,omitted
R2041 T3137 T3135 auxpass was,omitted
R2042 T3138 T3119 punct .,carried
R2043 T3140 T3141 compound P1,nuclease
R2044 T3141 T3142 nsubjpass nuclease,added
R2045 T3143 T3144 punct (,Roche
R2046 T3144 T3141 parataxis Roche,nuclease
R2047 T3145 T3146 nummod 0.3,U
R2048 T3146 T3144 dep U,Roche
R2049 T3147 T3144 punct ", ",Roche
R2050 T3148 T3144 punct ),Roche
R2051 T3149 T3142 auxpass was,added
R2052 T3150 T3142 cc and,added
R2053 T3151 T3152 nsubjpass incubation,continued
R2054 T3152 T3142 conj continued,added
R2055 T3153 T3152 auxpass was,continued
R2056 T3154 T3152 prep at,continued
R2057 T3155 T3156 nummod 37,°C
R2058 T3156 T3154 pobj °C,at
R2059 T3157 T3152 prep for,continued
R2060 T3158 T3159 nummod 1,min
R2061 T3159 T3157 pobj min,for
R2062 T3160 T3152 punct .,continued
R2063 T3162 T3163 nsubjpass Cleavage,stopped
R2064 T3163 T3165 ccomp stopped,incubated
R2065 T3164 T3163 auxpass was,stopped
R2066 T3166 T3163 prep by,stopped
R2067 T3167 T3166 pobj addition,by
R2068 T3168 T3167 prep of,addition
R2069 T3169 T3170 nummod 50,μl
R2070 T3170 T3168 pobj μl,of
R2071 T3171 T3170 prep of,μl
R2072 T3172 T3173 nummod 25,mM
R2073 T3173 T3174 compound mM,EDTA
R2074 T3174 T3171 pobj EDTA,of
R2075 T3175 T3174 cc and,EDTA
R2076 T3176 T3177 nummod 0.4,M
R2077 T3177 T3178 compound M,NaOH
R2078 T3178 T3174 conj NaOH,EDTA
R2079 T3179 T3165 punct ;,incubated
R2080 T3180 T3181 det the,mixture
R2081 T3181 T3165 nsubjpass mixture,incubated
R2082 T3182 T3165 auxpass was,incubated
R2083 T3183 T3165 prep at,incubated
R2084 T3184 T3185 compound room,temperature
R2085 T3185 T3183 pobj temperature,at
R2086 T3186 T3165 prep for,incubated
R2087 T3187 T3188 nummod 10,min
R2088 T3188 T3186 pobj min,for
R2089 T3189 T3188 acl followed,min
R2090 T3190 T3189 agent by,followed
R2091 T3191 T3192 det the,addition
R2092 T3192 T3190 pobj addition,by
R2093 T3193 T3192 prep of,addition
R2094 T3194 T3195 nummod 12,μl
R2095 T3195 T3193 pobj μl,of
R2096 T3196 T3195 prep of,μl
R2097 T3197 T3198 nummod 3,M
R2098 T3198 T3199 compound M,acetate
R2099 T3199 T3196 pobj acetate,of
R2100 T3200 T3199 compound sodium,acetate
R2101 T3201 T3202 punct (,pH
R2102 T3202 T3199 parataxis pH,acetate
R2103 T3203 T3202 nummod 4.8,pH
R2104 T3204 T3202 punct ),pH
R2105 T3205 T3165 punct .,incubated
R2106 T3207 T3208 nsubjpass Proteins,removed
R2107 T3209 T3208 auxpass were,removed
R2108 T3210 T3208 prep by,removed
R2109 T3211 T3212 compound phenol,chloroform
R2110 T3212 T3214 compound chloroform,extraction
R2111 T3213 T3212 punct –,chloroform
R2112 T3214 T3210 pobj extraction,by
R2113 T3215 T3208 punct ", ",removed
R2114 T3216 T3208 cc and,removed
R2115 T3217 T3218 nsubjpass DNAs,collected
R2116 T3218 T3208 conj collected,removed
R2117 T3219 T3218 auxpass were,collected
R2118 T3220 T3218 prep by,collected
R2119 T3221 T3222 compound ethanol,precipitation
R2120 T3222 T3220 pobj precipitation,by
R2121 T3223 T3222 acl followed,precipitation
R2122 T3224 T3223 agent by,followed
R2123 T3225 T3224 pobj wash,by
R2124 T3226 T3225 prep with,wash
R2125 T3227 T3228 nummod 70,%
R2126 T3228 T3229 compound %,ethanol
R2127 T3229 T3226 pobj ethanol,with
R2128 T3230 T3218 punct .,collected
R2129 T3232 T3233 det The,DNA
R2130 T3233 T3235 nsubjpass DNA,subjected
R2131 T3234 T3233 amod digested,DNA
R2132 T3236 T3235 auxpass was,subjected
R2133 T3237 T3235 prep to,subjected
R2134 T3238 T3237 pobj electrophoresis,to
R2135 T3239 T3238 prep through,electrophoresis
R2136 T3240 T3241 det a,gel
R2137 T3241 T3239 pobj gel,through
R2138 T3242 T3243 nummod 10,%
R2139 T3243 T3241 compound %,gel
R2140 T3244 T3242 cc or,10
R2141 T3245 T3242 conj 12,10
R2142 T3246 T3241 compound polyacrylamide,gel
R2143 T3247 T3241 compound sequencing,gel
R2144 T3248 T3241 acl containing,gel
R2145 T3249 T3250 nummod 7,M
R2146 T3250 T3251 compound M,urea
R2147 T3251 T3248 dobj urea,containing
R2148 T3252 T3238 punct ", ",electrophoresis
R2149 T3253 T3238 acl followed,electrophoresis
R2150 T3254 T3253 agent by,followed
R2151 T3255 T3254 pobj autoradiography,by
R2152 T3256 T3235 punct .,subjected