Id |
Subject |
Object |
Predicate |
Lexical cue |
T2636 |
0-2 |
IN |
denotes |
In |
T2638 |
3-6 |
NN |
denotes |
gel |
T2639 |
7-12 |
NN |
denotes |
shift |
T2640 |
13-19 |
NNS |
denotes |
assays |
T2641 |
19-21 |
, |
denotes |
, |
T2642 |
21-25 |
NN |
denotes |
Mcm4 |
T2644 |
25-26 |
HYPH |
denotes |
/ |
T2645 |
26-27 |
CD |
denotes |
6 |
T2646 |
27-28 |
HYPH |
denotes |
/ |
T2647 |
28-29 |
CD |
denotes |
7 |
T2643 |
30-38 |
NN |
denotes |
proteins |
T2648 |
39-43 |
VBD |
denotes |
were |
T2637 |
44-53 |
VBN |
denotes |
incubated |
T2649 |
54-56 |
IN |
denotes |
at |
T2650 |
57-59 |
CD |
denotes |
30 |
T2651 |
59-61 |
NN |
denotes |
°C |
T2652 |
62-65 |
IN |
denotes |
for |
T2653 |
66-68 |
CD |
denotes |
30 |
T2654 |
69-72 |
NN |
denotes |
min |
T2655 |
73-75 |
IN |
denotes |
in |
T2656 |
76-84 |
NN |
denotes |
reaction |
T2657 |
85-93 |
NNS |
denotes |
mixtures |
T2658 |
94-95 |
-LRB- |
denotes |
( |
T2660 |
95-97 |
CD |
denotes |
15 |
T2659 |
98-100 |
NN |
denotes |
μl |
T2661 |
100-101 |
-RRB- |
denotes |
) |
T2662 |
102-112 |
VBG |
denotes |
containing |
T2663 |
113-115 |
CD |
denotes |
25 |
T2664 |
116-118 |
NN |
denotes |
mM |
T2666 |
119-124 |
NN |
denotes |
HEPES |
T2667 |
124-125 |
HYPH |
denotes |
– |
T2665 |
125-129 |
NN |
denotes |
NaOH |
T2668 |
130-131 |
-LRB- |
denotes |
( |
T2669 |
131-133 |
NN |
denotes |
pH |
T2670 |
134-137 |
CD |
denotes |
7.5 |
T2671 |
137-138 |
-RRB- |
denotes |
) |
T2672 |
138-140 |
, |
denotes |
, |
T2673 |
140-142 |
CD |
denotes |
50 |
T2674 |
143-145 |
NN |
denotes |
mM |
T2676 |
146-152 |
NN |
denotes |
sodium |
T2675 |
153-160 |
NN |
denotes |
acetate |
T2677 |
160-162 |
, |
denotes |
, |
T2678 |
162-164 |
CD |
denotes |
10 |
T2679 |
165-167 |
NN |
denotes |
mM |
T2681 |
168-170 |
NN |
denotes |
Mg |
T2682 |
170-171 |
-LRB- |
denotes |
( |
T2680 |
171-177 |
NN |
denotes |
CH3COO |
T2683 |
177-178 |
-RRB- |
denotes |
) |
T2684 |
178-179 |
CD |
denotes |
2 |
T2685 |
179-181 |
, |
denotes |
, |
T2686 |
181-182 |
CD |
denotes |
1 |
T2687 |
183-185 |
NN |
denotes |
mM |
T2688 |
186-189 |
NN |
denotes |
DTT |
T2689 |
189-191 |
, |
denotes |
, |
T2690 |
191-195 |
CD |
denotes |
0.25 |
T2691 |
196-198 |
NN |
denotes |
μg |
T2693 |
198-199 |
SYM |
denotes |
/ |
T2694 |
199-201 |
NN |
denotes |
ml |
T2692 |
202-205 |
NN |
denotes |
BSA |
T2695 |
205-207 |
, |
denotes |
, |
T2696 |
207-210 |
CD |
denotes |
0.5 |
T2697 |
211-213 |
NN |
denotes |
mM |
T2699 |
214-217 |
NN |
denotes |
ATP |
T2700 |
217-218 |
HYPH |
denotes |
- |
T2701 |
218-219 |
SYM |
denotes |
γ |
T2702 |
219-220 |
HYPH |
denotes |
- |
T2698 |
220-221 |
NN |
denotes |
S |
T2703 |
222-225 |
CC |
denotes |
and |
T2704 |
226-233 |
VBN |
denotes |
labeled |
T2705 |
234-244 |
NNS |
denotes |
substrates |
T2706 |
245-247 |
IN |
denotes |
of |
T2707 |
248-251 |
DT |
denotes |
the |
T2708 |
252-258 |
NN |
denotes |
amount |
T2709 |
259-268 |
VBN |
denotes |
indicated |
T2710 |
268-269 |
. |
denotes |
. |
T2711 |
269-495 |
sentence |
denotes |
After addition of 2 μl of 50% glycerol, the reaction mixtures were directly applied on a polyacrylamide gel containing 6 mM magnesium acetate and 5% glycerol in 0.5× TBE, and electrophoresis was conducted at room temperature. |
T2712 |
270-275 |
IN |
denotes |
After |
T2714 |
276-284 |
NN |
denotes |
addition |
T2715 |
285-287 |
IN |
denotes |
of |
T2716 |
288-289 |
CD |
denotes |
2 |
T2717 |
290-292 |
NN |
denotes |
μl |
T2718 |
293-295 |
IN |
denotes |
of |
T2719 |
296-298 |
CD |
denotes |
50 |
T2720 |
298-299 |
NN |
denotes |
% |
T2721 |
300-308 |
NN |
denotes |
glycerol |
T2722 |
308-310 |
, |
denotes |
, |
T2723 |
310-313 |
DT |
denotes |
the |
T2725 |
314-322 |
NN |
denotes |
reaction |
T2724 |
323-331 |
NNS |
denotes |
mixtures |
T2726 |
332-336 |
VBD |
denotes |
were |
T2727 |
337-345 |
RB |
denotes |
directly |
T2713 |
346-353 |
VBN |
denotes |
applied |
T2728 |
354-356 |
IN |
denotes |
on |
T2729 |
357-358 |
DT |
denotes |
a |
T2731 |
359-373 |
NN |
denotes |
polyacrylamide |
T2730 |
374-377 |
NN |
denotes |
gel |
T2732 |
378-388 |
VBG |
denotes |
containing |
T2733 |
389-390 |
CD |
denotes |
6 |
T2734 |
391-393 |
NN |
denotes |
mM |
T2736 |
394-403 |
NN |
denotes |
magnesium |
T2735 |
404-411 |
NN |
denotes |
acetate |
T2737 |
412-415 |
CC |
denotes |
and |
T2738 |
416-417 |
CD |
denotes |
5 |
T2739 |
417-418 |
NN |
denotes |
% |
T2740 |
419-427 |
NN |
denotes |
glycerol |
T2741 |
428-430 |
IN |
denotes |
in |
T2742 |
431-434 |
CD |
denotes |
0.5 |
T2744 |
434-435 |
SYM |
denotes |
× |
T2743 |
436-439 |
NN |
denotes |
TBE |
T2745 |
439-441 |
, |
denotes |
, |
T2746 |
441-444 |
CC |
denotes |
and |
T2747 |
445-460 |
NN |
denotes |
electrophoresis |
T2749 |
461-464 |
VBD |
denotes |
was |
T2748 |
465-474 |
VBN |
denotes |
conducted |
T2750 |
475-477 |
IN |
denotes |
at |
T2751 |
478-482 |
NN |
denotes |
room |
T2752 |
483-494 |
NN |
denotes |
temperature |
T2753 |
494-495 |
. |
denotes |
. |
T2754 |
495-713 |
sentence |
denotes |
For DNA helicase assays, the reaction mixtures of the gel shift assays, as described above, were incubated at 30°C for 15 min, and then the ATP was added at 10 mM, followed by incubation at 37°C for additional 30 min. |
T2755 |
496-499 |
IN |
denotes |
For |
T2757 |
500-503 |
NN |
denotes |
DNA |
T2759 |
504-512 |
NN |
denotes |
helicase |
T2758 |
513-519 |
NNS |
denotes |
assays |
T2760 |
519-521 |
, |
denotes |
, |
T2761 |
521-524 |
DT |
denotes |
the |
T2763 |
525-533 |
NN |
denotes |
reaction |
T2762 |
534-542 |
NNS |
denotes |
mixtures |
T2764 |
543-545 |
IN |
denotes |
of |
T2765 |
546-549 |
DT |
denotes |
the |
T2767 |
550-553 |
NN |
denotes |
gel |
T2768 |
554-559 |
NN |
denotes |
shift |
T2766 |
560-566 |
NNS |
denotes |
assays |
T2769 |
566-568 |
, |
denotes |
, |
T2770 |
568-570 |
IN |
denotes |
as |
T2771 |
571-580 |
VBN |
denotes |
described |
T2772 |
581-586 |
RB |
denotes |
above |
T2773 |
586-588 |
, |
denotes |
, |
T2774 |
588-592 |
VBD |
denotes |
were |
T2756 |
593-602 |
VBN |
denotes |
incubated |
T2775 |
603-605 |
IN |
denotes |
at |
T2776 |
606-608 |
CD |
denotes |
30 |
T2777 |
608-610 |
NN |
denotes |
°C |
T2778 |
611-614 |
IN |
denotes |
for |
T2779 |
615-617 |
CD |
denotes |
15 |
T2780 |
618-621 |
NN |
denotes |
min |
T2781 |
621-623 |
, |
denotes |
, |
T2782 |
623-626 |
CC |
denotes |
and |
T2783 |
627-631 |
RB |
denotes |
then |
T2784 |
632-635 |
DT |
denotes |
the |
T2785 |
636-639 |
NN |
denotes |
ATP |
T2787 |
640-643 |
VBD |
denotes |
was |
T2786 |
644-649 |
VBN |
denotes |
added |
T2788 |
650-652 |
IN |
denotes |
at |
T2789 |
653-655 |
CD |
denotes |
10 |
T2790 |
656-658 |
NN |
denotes |
mM |
T2791 |
658-660 |
, |
denotes |
, |
T2792 |
660-668 |
VBN |
denotes |
followed |
T2793 |
669-671 |
IN |
denotes |
by |
T2794 |
672-682 |
NN |
denotes |
incubation |
T2795 |
683-685 |
IN |
denotes |
at |
T2796 |
686-688 |
CD |
denotes |
37 |
T2797 |
688-690 |
NN |
denotes |
°C |
T2798 |
691-694 |
IN |
denotes |
for |
T2799 |
695-705 |
JJ |
denotes |
additional |
T2801 |
706-708 |
CD |
denotes |
30 |
T2800 |
709-712 |
NN |
denotes |
min |
T2802 |
712-713 |
. |
denotes |
. |
T2803 |
713-848 |
sentence |
denotes |
The reactions were terminated by addition of EDTA (20 mM), SDS (0.1%) and 2 μg proteinase K, and were incubated for additional 15 min. |
T2804 |
714-717 |
DT |
denotes |
The |
T2805 |
718-727 |
NNS |
denotes |
reactions |
T2807 |
728-732 |
VBD |
denotes |
were |
T2806 |
733-743 |
VBN |
denotes |
terminated |
T2808 |
744-746 |
IN |
denotes |
by |
T2809 |
747-755 |
NN |
denotes |
addition |
T2810 |
756-758 |
IN |
denotes |
of |
T2811 |
759-763 |
NN |
denotes |
EDTA |
T2812 |
764-765 |
-LRB- |
denotes |
( |
T2814 |
765-767 |
CD |
denotes |
20 |
T2813 |
768-770 |
NN |
denotes |
mM |
T2815 |
770-771 |
-RRB- |
denotes |
) |
T2816 |
771-773 |
, |
denotes |
, |
T2817 |
773-776 |
NN |
denotes |
SDS |
T2818 |
777-778 |
-LRB- |
denotes |
( |
T2820 |
778-781 |
CD |
denotes |
0.1 |
T2819 |
781-782 |
NN |
denotes |
% |
T2821 |
782-783 |
-RRB- |
denotes |
) |
T2822 |
784-787 |
CC |
denotes |
and |
T2823 |
788-789 |
CD |
denotes |
2 |
T2824 |
790-792 |
NN |
denotes |
μg |
T2826 |
793-803 |
NN |
denotes |
proteinase |
T2825 |
804-805 |
NN |
denotes |
K |
T2827 |
805-807 |
, |
denotes |
, |
T2828 |
807-810 |
CC |
denotes |
and |
T2829 |
811-815 |
VBD |
denotes |
were |
T2830 |
816-825 |
VBN |
denotes |
incubated |
T2831 |
826-829 |
IN |
denotes |
for |
T2832 |
830-840 |
JJ |
denotes |
additional |
T2834 |
841-843 |
CD |
denotes |
15 |
T2833 |
844-847 |
NN |
denotes |
min |
T2835 |
847-848 |
. |
denotes |
. |
T2836 |
848-941 |
sentence |
denotes |
The samples were separated by electrophoresis on nondenaturing polyacrylamide gel in 1× TBE. |
T2837 |
849-852 |
DT |
denotes |
The |
T2838 |
853-860 |
NNS |
denotes |
samples |
T2840 |
861-865 |
VBD |
denotes |
were |
T2839 |
866-875 |
VBN |
denotes |
separated |
T2841 |
876-878 |
IN |
denotes |
by |
T2842 |
879-894 |
NN |
denotes |
electrophoresis |
T2843 |
895-897 |
IN |
denotes |
on |
T2844 |
898-911 |
JJ |
denotes |
nondenaturing |
T2846 |
912-926 |
NN |
denotes |
polyacrylamide |
T2845 |
927-930 |
NN |
denotes |
gel |
T2847 |
931-933 |
IN |
denotes |
in |
T2848 |
934-935 |
CD |
denotes |
1 |
T2850 |
935-936 |
SYM |
denotes |
× |
T2849 |
937-940 |
NN |
denotes |
TBE |
T2851 |
940-941 |
. |
denotes |
. |
R1673 |
T2636 |
T2637 |
prep |
In,incubated |
R1674 |
T2638 |
T2639 |
compound |
gel,shift |
R1675 |
T2639 |
T2640 |
compound |
shift,assays |
R1676 |
T2640 |
T2636 |
pobj |
assays,In |
R1677 |
T2641 |
T2637 |
punct |
", ",incubated |
R1678 |
T2642 |
T2643 |
nmod |
Mcm4,proteins |
R1679 |
T2643 |
T2637 |
nsubjpass |
proteins,incubated |
R1680 |
T2644 |
T2642 |
punct |
/,Mcm4 |
R1681 |
T2645 |
T2642 |
nummod |
6,Mcm4 |
R1682 |
T2646 |
T2642 |
punct |
/,Mcm4 |
R1683 |
T2647 |
T2642 |
nummod |
7,Mcm4 |
R1684 |
T2648 |
T2637 |
auxpass |
were,incubated |
R1685 |
T2649 |
T2637 |
prep |
at,incubated |
R1686 |
T2650 |
T2651 |
nummod |
30,°C |
R1687 |
T2651 |
T2649 |
pobj |
°C,at |
R1688 |
T2652 |
T2637 |
prep |
for,incubated |
R1689 |
T2653 |
T2654 |
nummod |
30,min |
R1690 |
T2654 |
T2652 |
pobj |
min,for |
R1691 |
T2655 |
T2637 |
prep |
in,incubated |
R1692 |
T2656 |
T2657 |
compound |
reaction,mixtures |
R1693 |
T2657 |
T2655 |
pobj |
mixtures,in |
R1694 |
T2658 |
T2659 |
punct |
(,μl |
R1695 |
T2659 |
T2657 |
parataxis |
μl,mixtures |
R1696 |
T2660 |
T2659 |
nummod |
15,μl |
R1697 |
T2661 |
T2659 |
punct |
),μl |
R1698 |
T2662 |
T2657 |
acl |
containing,mixtures |
R1699 |
T2663 |
T2664 |
nummod |
25,mM |
R1700 |
T2664 |
T2665 |
compound |
mM,NaOH |
R1701 |
T2665 |
T2662 |
dobj |
NaOH,containing |
R1702 |
T2666 |
T2665 |
compound |
HEPES,NaOH |
R1703 |
T2667 |
T2665 |
punct |
–,NaOH |
R1704 |
T2668 |
T2669 |
punct |
(,pH |
R1705 |
T2669 |
T2665 |
parataxis |
pH,NaOH |
R1706 |
T2670 |
T2669 |
nummod |
7.5,pH |
R1707 |
T2671 |
T2669 |
punct |
),pH |
R1708 |
T2672 |
T2665 |
punct |
", ",NaOH |
R1709 |
T2673 |
T2674 |
nummod |
50,mM |
R1710 |
T2674 |
T2675 |
compound |
mM,acetate |
R1711 |
T2675 |
T2665 |
conj |
acetate,NaOH |
R1712 |
T2676 |
T2675 |
compound |
sodium,acetate |
R1713 |
T2677 |
T2675 |
punct |
", ",acetate |
R1714 |
T2678 |
T2679 |
nummod |
10,mM |
R1715 |
T2679 |
T2680 |
nmod |
mM,CH3COO |
R1716 |
T2680 |
T2675 |
conj |
CH3COO,acetate |
R1717 |
T2681 |
T2680 |
nmod |
Mg,CH3COO |
R1718 |
T2682 |
T2680 |
punct |
(,CH3COO |
R1719 |
T2683 |
T2680 |
punct |
),CH3COO |
R1720 |
T2684 |
T2680 |
nummod |
2,CH3COO |
R1721 |
T2685 |
T2680 |
punct |
", ",CH3COO |
R1722 |
T2686 |
T2687 |
nummod |
1,mM |
R1723 |
T2687 |
T2688 |
compound |
mM,DTT |
R1724 |
T2688 |
T2680 |
conj |
DTT,CH3COO |
R1725 |
T2689 |
T2688 |
punct |
", ",DTT |
R1726 |
T2690 |
T2691 |
nummod |
0.25,μg |
R1727 |
T2691 |
T2692 |
nmod |
μg,BSA |
R1728 |
T2692 |
T2688 |
conj |
BSA,DTT |
R1729 |
T2693 |
T2694 |
punct |
/,ml |
R1730 |
T2694 |
T2691 |
prep |
ml,μg |
R1731 |
T2695 |
T2692 |
punct |
", ",BSA |
R1732 |
T2696 |
T2697 |
nummod |
0.5,mM |
R1733 |
T2697 |
T2698 |
nmod |
mM,S |
R1734 |
T2698 |
T2692 |
conj |
S,BSA |
R1735 |
T2699 |
T2698 |
nmod |
ATP,S |
R1736 |
T2700 |
T2698 |
punct |
-,S |
R1737 |
T2701 |
T2698 |
punct |
γ,S |
R1738 |
T2702 |
T2698 |
punct |
-,S |
R1739 |
T2703 |
T2698 |
cc |
and,S |
R1740 |
T2704 |
T2705 |
amod |
labeled,substrates |
R1741 |
T2705 |
T2698 |
conj |
substrates,S |
R1742 |
T2706 |
T2705 |
prep |
of,substrates |
R1743 |
T2707 |
T2708 |
det |
the,amount |
R1744 |
T2708 |
T2706 |
pobj |
amount,of |
R1745 |
T2709 |
T2708 |
acl |
indicated,amount |
R1746 |
T2710 |
T2637 |
punct |
.,incubated |
R1747 |
T2712 |
T2713 |
prep |
After,applied |
R1748 |
T2714 |
T2712 |
pobj |
addition,After |
R1749 |
T2715 |
T2714 |
prep |
of,addition |
R1750 |
T2716 |
T2717 |
nummod |
2,μl |
R1751 |
T2717 |
T2715 |
pobj |
μl,of |
R1752 |
T2718 |
T2717 |
prep |
of,μl |
R1753 |
T2719 |
T2720 |
nummod |
50,% |
R1754 |
T2720 |
T2721 |
compound |
%,glycerol |
R1755 |
T2721 |
T2718 |
pobj |
glycerol,of |
R1756 |
T2722 |
T2713 |
punct |
", ",applied |
R1757 |
T2723 |
T2724 |
det |
the,mixtures |
R1758 |
T2724 |
T2713 |
nsubjpass |
mixtures,applied |
R1759 |
T2725 |
T2724 |
compound |
reaction,mixtures |
R1760 |
T2726 |
T2713 |
auxpass |
were,applied |
R1761 |
T2727 |
T2713 |
advmod |
directly,applied |
R1762 |
T2728 |
T2713 |
prep |
on,applied |
R1763 |
T2729 |
T2730 |
det |
a,gel |
R1764 |
T2730 |
T2728 |
pobj |
gel,on |
R1765 |
T2731 |
T2730 |
compound |
polyacrylamide,gel |
R1766 |
T2732 |
T2730 |
acl |
containing,gel |
R1767 |
T2733 |
T2734 |
nummod |
6,mM |
R1768 |
T2734 |
T2735 |
compound |
mM,acetate |
R1769 |
T2735 |
T2732 |
dobj |
acetate,containing |
R1770 |
T2736 |
T2735 |
compound |
magnesium,acetate |
R1771 |
T2737 |
T2735 |
cc |
and,acetate |
R1772 |
T2738 |
T2739 |
nummod |
5,% |
R1773 |
T2739 |
T2740 |
compound |
%,glycerol |
R1774 |
T2740 |
T2735 |
conj |
glycerol,acetate |
R1775 |
T2741 |
T2732 |
prep |
in,containing |
R1776 |
T2742 |
T2743 |
nummod |
0.5,TBE |
R1777 |
T2743 |
T2741 |
pobj |
TBE,in |
R1778 |
T2744 |
T2742 |
punct |
×,0.5 |
R1779 |
T2745 |
T2713 |
punct |
", ",applied |
R1780 |
T2746 |
T2713 |
cc |
and,applied |
R1781 |
T2747 |
T2748 |
nsubjpass |
electrophoresis,conducted |
R1782 |
T2748 |
T2713 |
conj |
conducted,applied |
R1783 |
T2749 |
T2748 |
auxpass |
was,conducted |
R1784 |
T2750 |
T2748 |
prep |
at,conducted |
R1785 |
T2751 |
T2752 |
compound |
room,temperature |
R1786 |
T2752 |
T2750 |
pobj |
temperature,at |
R1787 |
T2753 |
T2748 |
punct |
.,conducted |
R1788 |
T2755 |
T2756 |
prep |
For,incubated |
R1789 |
T2757 |
T2758 |
compound |
DNA,assays |
R1790 |
T2758 |
T2755 |
pobj |
assays,For |
R1791 |
T2759 |
T2758 |
compound |
helicase,assays |
R1792 |
T2760 |
T2756 |
punct |
", ",incubated |
R1793 |
T2761 |
T2762 |
det |
the,mixtures |
R1794 |
T2762 |
T2756 |
nsubjpass |
mixtures,incubated |
R1795 |
T2763 |
T2762 |
compound |
reaction,mixtures |
R1796 |
T2764 |
T2762 |
prep |
of,mixtures |
R1797 |
T2765 |
T2766 |
det |
the,assays |
R1798 |
T2766 |
T2764 |
pobj |
assays,of |
R1799 |
T2767 |
T2768 |
compound |
gel,shift |
R1800 |
T2768 |
T2766 |
compound |
shift,assays |
R1801 |
T2769 |
T2756 |
punct |
", ",incubated |
R1802 |
T2770 |
T2771 |
mark |
as,described |
R1803 |
T2771 |
T2756 |
advcl |
described,incubated |
R1804 |
T2772 |
T2771 |
advmod |
above,described |
R1805 |
T2773 |
T2756 |
punct |
", ",incubated |
R1806 |
T2774 |
T2756 |
auxpass |
were,incubated |
R1807 |
T2775 |
T2756 |
prep |
at,incubated |
R1808 |
T2776 |
T2777 |
nummod |
30,°C |
R1809 |
T2777 |
T2775 |
pobj |
°C,at |
R1810 |
T2778 |
T2756 |
prep |
for,incubated |
R1811 |
T2779 |
T2780 |
nummod |
15,min |
R1812 |
T2780 |
T2778 |
pobj |
min,for |
R1813 |
T2781 |
T2756 |
punct |
", ",incubated |
R1814 |
T2782 |
T2756 |
cc |
and,incubated |
R1815 |
T2783 |
T2782 |
advmod |
then,and |
R1816 |
T2784 |
T2785 |
det |
the,ATP |
R1817 |
T2785 |
T2786 |
nsubjpass |
ATP,added |
R1818 |
T2786 |
T2756 |
conj |
added,incubated |
R1819 |
T2787 |
T2786 |
auxpass |
was,added |
R1820 |
T2788 |
T2786 |
prep |
at,added |
R1821 |
T2789 |
T2790 |
nummod |
10,mM |
R1822 |
T2790 |
T2788 |
pobj |
mM,at |
R1823 |
T2791 |
T2786 |
punct |
", ",added |
R1824 |
T2792 |
T2786 |
advcl |
followed,added |
R1825 |
T2793 |
T2792 |
agent |
by,followed |
R1826 |
T2794 |
T2793 |
pobj |
incubation,by |
R1827 |
T2795 |
T2794 |
prep |
at,incubation |
R1828 |
T2796 |
T2797 |
nummod |
37,°C |
R1829 |
T2797 |
T2795 |
pobj |
°C,at |
R1830 |
T2798 |
T2794 |
prep |
for,incubation |
R1831 |
T2799 |
T2800 |
amod |
additional,min |
R1832 |
T2800 |
T2798 |
pobj |
min,for |
R1833 |
T2801 |
T2800 |
nummod |
30,min |
R1834 |
T2802 |
T2786 |
punct |
.,added |
R1835 |
T2804 |
T2805 |
det |
The,reactions |
R1836 |
T2805 |
T2806 |
nsubjpass |
reactions,terminated |
R1837 |
T2807 |
T2806 |
auxpass |
were,terminated |
R1838 |
T2808 |
T2806 |
agent |
by,terminated |
R1839 |
T2809 |
T2808 |
pobj |
addition,by |
R1840 |
T2810 |
T2809 |
prep |
of,addition |
R1841 |
T2811 |
T2810 |
pobj |
EDTA,of |
R1842 |
T2812 |
T2813 |
punct |
(,mM |
R1843 |
T2813 |
T2811 |
parataxis |
mM,EDTA |
R1844 |
T2814 |
T2813 |
nummod |
20,mM |
R1845 |
T2815 |
T2813 |
punct |
),mM |
R1846 |
T2816 |
T2811 |
punct |
", ",EDTA |
R1847 |
T2817 |
T2811 |
conj |
SDS,EDTA |
R1848 |
T2818 |
T2819 |
punct |
(,% |
R1849 |
T2819 |
T2817 |
parataxis |
%,SDS |
R1850 |
T2820 |
T2819 |
nummod |
0.1,% |
R1851 |
T2821 |
T2819 |
punct |
),% |
R1852 |
T2822 |
T2817 |
cc |
and,SDS |
R1853 |
T2823 |
T2824 |
nummod |
2,μg |
R1854 |
T2824 |
T2825 |
compound |
μg,K |
R1855 |
T2825 |
T2817 |
conj |
K,SDS |
R1856 |
T2826 |
T2825 |
compound |
proteinase,K |
R1857 |
T2827 |
T2806 |
punct |
", ",terminated |
R1858 |
T2828 |
T2806 |
cc |
and,terminated |
R1859 |
T2829 |
T2830 |
auxpass |
were,incubated |
R1860 |
T2830 |
T2806 |
conj |
incubated,terminated |
R1861 |
T2831 |
T2830 |
prep |
for,incubated |
R1862 |
T2832 |
T2833 |
amod |
additional,min |
R1863 |
T2833 |
T2831 |
pobj |
min,for |
R1864 |
T2834 |
T2833 |
nummod |
15,min |
R1865 |
T2835 |
T2806 |
punct |
.,terminated |
R1866 |
T2837 |
T2838 |
det |
The,samples |
R1867 |
T2838 |
T2839 |
nsubjpass |
samples,separated |
R1868 |
T2840 |
T2839 |
auxpass |
were,separated |
R1869 |
T2841 |
T2839 |
prep |
by,separated |
R1870 |
T2842 |
T2841 |
pobj |
electrophoresis,by |
R1871 |
T2843 |
T2839 |
prep |
on,separated |
R1872 |
T2844 |
T2845 |
amod |
nondenaturing,gel |
R1873 |
T2845 |
T2843 |
pobj |
gel,on |
R1874 |
T2846 |
T2845 |
compound |
polyacrylamide,gel |
R1875 |
T2847 |
T2845 |
prep |
in,gel |
R1876 |
T2848 |
T2849 |
nummod |
1,TBE |
R1877 |
T2849 |
T2847 |
pobj |
TBE,in |
R1878 |
T2850 |
T2848 |
punct |
×,1 |
R1879 |
T2851 |
T2839 |
punct |
.,separated |