| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1667 |
0-10 |
NN |
denotes |
Expression |
| T1668 |
11-14 |
CC |
denotes |
and |
| T1669 |
15-27 |
NN |
denotes |
purification |
| T1670 |
28-30 |
IN |
denotes |
of |
| T1671 |
31-36 |
NN |
denotes |
mouse |
| T1673 |
37-41 |
NN |
denotes |
Mcm4 |
| T1674 |
41-42 |
HYPH |
denotes |
/ |
| T1675 |
42-43 |
CD |
denotes |
6 |
| T1676 |
43-44 |
HYPH |
denotes |
/ |
| T1677 |
44-45 |
CD |
denotes |
7 |
| T1672 |
46-53 |
NN |
denotes |
complex |
| T1678 |
53-163 |
sentence |
denotes |
The recombinant baculoviruses expressing His6-Mcm4/Mcm6 or Mcm7-FLAG proteins were previously described (11). |
| T1679 |
54-57 |
DT |
denotes |
The |
| T1681 |
58-69 |
JJ |
denotes |
recombinant |
| T1680 |
70-83 |
NNS |
denotes |
baculoviruses |
| T1683 |
84-94 |
VBG |
denotes |
expressing |
| T1684 |
95-99 |
NN |
denotes |
His6 |
| T1686 |
99-100 |
HYPH |
denotes |
- |
| T1687 |
100-104 |
NN |
denotes |
Mcm4 |
| T1688 |
104-105 |
HYPH |
denotes |
/ |
| T1685 |
105-109 |
NN |
denotes |
Mcm6 |
| T1690 |
110-112 |
CC |
denotes |
or |
| T1691 |
113-117 |
NN |
denotes |
Mcm7 |
| T1693 |
117-118 |
HYPH |
denotes |
- |
| T1692 |
118-122 |
NN |
denotes |
FLAG |
| T1689 |
123-131 |
NN |
denotes |
proteins |
| T1694 |
132-136 |
VBD |
denotes |
were |
| T1695 |
137-147 |
RB |
denotes |
previously |
| T1682 |
148-157 |
VBN |
denotes |
described |
| T1696 |
158-159 |
-LRB- |
denotes |
( |
| T1697 |
159-161 |
CD |
denotes |
11 |
| T1698 |
161-162 |
-RRB- |
denotes |
) |
| T1699 |
162-163 |
. |
denotes |
. |
| T1700 |
163-315 |
sentence |
denotes |
Sf9 and High five insect cells were cultured at 27°C in Sf-900 II SFM (Life Technologies, Inc.) and EX-CELL 405 medium (JRH Biosciences), respectively. |
| T1701 |
164-167 |
NN |
denotes |
Sf9 |
| T1703 |
168-171 |
CC |
denotes |
and |
| T1704 |
172-176 |
JJ |
denotes |
High |
| T1705 |
177-181 |
CD |
denotes |
five |
| T1706 |
182-188 |
NN |
denotes |
insect |
| T1702 |
189-194 |
NNS |
denotes |
cells |
| T1708 |
195-199 |
VBD |
denotes |
were |
| T1707 |
200-208 |
VBN |
denotes |
cultured |
| T1709 |
209-211 |
IN |
denotes |
at |
| T1710 |
212-214 |
CD |
denotes |
27 |
| T1711 |
214-216 |
NN |
denotes |
°C |
| T1712 |
217-219 |
IN |
denotes |
in |
| T1713 |
220-222 |
NN |
denotes |
Sf |
| T1715 |
222-223 |
HYPH |
denotes |
- |
| T1716 |
223-226 |
CD |
denotes |
900 |
| T1717 |
227-229 |
CD |
denotes |
II |
| T1714 |
230-233 |
NN |
denotes |
SFM |
| T1718 |
234-235 |
-LRB- |
denotes |
( |
| T1720 |
235-239 |
NNP |
denotes |
Life |
| T1721 |
240-252 |
NNP |
denotes |
Technologies |
| T1722 |
252-254 |
, |
denotes |
, |
| T1719 |
254-258 |
NNP |
denotes |
Inc. |
| T1723 |
258-259 |
-RRB- |
denotes |
) |
| T1724 |
260-263 |
CC |
denotes |
and |
| T1725 |
264-266 |
NN |
denotes |
EX |
| T1727 |
266-267 |
HYPH |
denotes |
- |
| T1726 |
267-271 |
NN |
denotes |
CELL |
| T1729 |
272-275 |
CD |
denotes |
405 |
| T1728 |
276-282 |
NN |
denotes |
medium |
| T1730 |
283-284 |
-LRB- |
denotes |
( |
| T1732 |
284-287 |
NNP |
denotes |
JRH |
| T1731 |
288-299 |
NNP |
denotes |
Biosciences |
| T1733 |
299-300 |
-RRB- |
denotes |
) |
| T1734 |
300-302 |
, |
denotes |
, |
| T1735 |
302-314 |
RB |
denotes |
respectively |
| T1736 |
314-315 |
. |
denotes |
. |
| T1737 |
315-537 |
sentence |
denotes |
For expression of the Mcm4/6/7 proteins, High five cells were coinfected with recombinant baculoviruses expressing the His6-Mcm4/Mcm6 proteins and those expressing the Mcm7-FLAG, and were collected at 48 h post-infection. |
| T1738 |
316-319 |
IN |
denotes |
For |
| T1740 |
320-330 |
NN |
denotes |
expression |
| T1741 |
331-333 |
IN |
denotes |
of |
| T1742 |
334-337 |
DT |
denotes |
the |
| T1744 |
338-342 |
NN |
denotes |
Mcm4 |
| T1745 |
342-343 |
HYPH |
denotes |
/ |
| T1746 |
343-344 |
CD |
denotes |
6 |
| T1747 |
344-345 |
HYPH |
denotes |
/ |
| T1748 |
345-346 |
CD |
denotes |
7 |
| T1743 |
347-355 |
NN |
denotes |
proteins |
| T1749 |
355-357 |
, |
denotes |
, |
| T1750 |
357-361 |
JJ |
denotes |
High |
| T1751 |
362-366 |
CD |
denotes |
five |
| T1752 |
367-372 |
NNS |
denotes |
cells |
| T1753 |
373-377 |
VBD |
denotes |
were |
| T1739 |
378-388 |
VBN |
denotes |
coinfected |
| T1754 |
389-393 |
IN |
denotes |
with |
| T1755 |
394-405 |
JJ |
denotes |
recombinant |
| T1756 |
406-419 |
NNS |
denotes |
baculoviruses |
| T1757 |
420-430 |
VBG |
denotes |
expressing |
| T1758 |
431-434 |
DT |
denotes |
the |
| T1760 |
435-439 |
NN |
denotes |
His6 |
| T1762 |
439-440 |
HYPH |
denotes |
- |
| T1763 |
440-444 |
NN |
denotes |
Mcm4 |
| T1764 |
444-445 |
HYPH |
denotes |
/ |
| T1761 |
445-449 |
NN |
denotes |
Mcm6 |
| T1759 |
450-458 |
NN |
denotes |
proteins |
| T1765 |
459-462 |
CC |
denotes |
and |
| T1766 |
463-468 |
DT |
denotes |
those |
| T1767 |
469-479 |
VBG |
denotes |
expressing |
| T1768 |
480-483 |
DT |
denotes |
the |
| T1770 |
484-488 |
NN |
denotes |
Mcm7 |
| T1771 |
488-489 |
HYPH |
denotes |
- |
| T1769 |
489-493 |
NN |
denotes |
FLAG |
| T1772 |
493-495 |
, |
denotes |
, |
| T1773 |
495-498 |
CC |
denotes |
and |
| T1774 |
499-503 |
VBD |
denotes |
were |
| T1775 |
504-513 |
VBN |
denotes |
collected |
| T1776 |
514-516 |
IN |
denotes |
at |
| T1777 |
517-519 |
CD |
denotes |
48 |
| T1778 |
520-521 |
NN |
denotes |
h |
| T1779 |
522-536 |
RB |
denotes |
post-infection |
| T1780 |
536-537 |
. |
denotes |
. |
| T1781 |
537-616 |
sentence |
denotes |
The recombinant Mcm4/6/7 complexes were purified as previously described (12). |
| T1782 |
538-541 |
DT |
denotes |
The |
| T1784 |
542-553 |
JJ |
denotes |
recombinant |
| T1785 |
554-558 |
NN |
denotes |
Mcm4 |
| T1786 |
558-559 |
HYPH |
denotes |
/ |
| T1787 |
559-560 |
CD |
denotes |
6 |
| T1788 |
560-561 |
HYPH |
denotes |
/ |
| T1789 |
561-562 |
CD |
denotes |
7 |
| T1783 |
563-572 |
NNS |
denotes |
complexes |
| T1791 |
573-577 |
VBD |
denotes |
were |
| T1790 |
578-586 |
VBN |
denotes |
purified |
| T1792 |
587-589 |
IN |
denotes |
as |
| T1794 |
590-600 |
RB |
denotes |
previously |
| T1793 |
601-610 |
VBN |
denotes |
described |
| T1795 |
611-612 |
-LRB- |
denotes |
( |
| T1796 |
612-614 |
CD |
denotes |
12 |
| T1797 |
614-615 |
-RRB- |
denotes |
) |
| T1798 |
615-616 |
. |
denotes |
. |
| R1000 |
T1673 |
T1672 |
nmod |
Mcm4,complex |
| R1001 |
T1674 |
T1673 |
punct |
/,Mcm4 |
| R1002 |
T1675 |
T1673 |
nummod |
6,Mcm4 |
| R1003 |
T1676 |
T1673 |
punct |
/,Mcm4 |
| R1004 |
T1677 |
T1673 |
nummod |
7,Mcm4 |
| R1005 |
T1679 |
T1680 |
det |
The,baculoviruses |
| R1006 |
T1680 |
T1682 |
nsubjpass |
baculoviruses,described |
| R1007 |
T1681 |
T1680 |
amod |
recombinant,baculoviruses |
| R1008 |
T1683 |
T1680 |
acl |
expressing,baculoviruses |
| R1009 |
T1684 |
T1685 |
nmod |
His6,Mcm6 |
| R1010 |
T1685 |
T1689 |
nmod |
Mcm6,proteins |
| R1011 |
T1686 |
T1685 |
punct |
-,Mcm6 |
| R1012 |
T1687 |
T1685 |
nmod |
Mcm4,Mcm6 |
| R1013 |
T1688 |
T1685 |
punct |
/,Mcm6 |
| R1014 |
T1689 |
T1683 |
dobj |
proteins,expressing |
| R1015 |
T1690 |
T1685 |
cc |
or,Mcm6 |
| R1016 |
T1691 |
T1692 |
compound |
Mcm7,FLAG |
| R1017 |
T1692 |
T1685 |
conj |
FLAG,Mcm6 |
| R1018 |
T1693 |
T1692 |
punct |
-,FLAG |
| R1019 |
T1694 |
T1682 |
auxpass |
were,described |
| R1020 |
T1695 |
T1682 |
advmod |
previously,described |
| R1021 |
T1696 |
T1697 |
punct |
(,11 |
| R1022 |
T1697 |
T1682 |
parataxis |
11,described |
| R1023 |
T1698 |
T1697 |
punct |
),11 |
| R1024 |
T1699 |
T1682 |
punct |
.,described |
| R1025 |
T1701 |
T1702 |
nmod |
Sf9,cells |
| R1026 |
T1702 |
T1707 |
nsubjpass |
cells,cultured |
| R1027 |
T1703 |
T1701 |
cc |
and,Sf9 |
| R1028 |
T1704 |
T1705 |
amod |
High,five |
| R1029 |
T1705 |
T1701 |
conj |
five,Sf9 |
| R1030 |
T1706 |
T1702 |
compound |
insect,cells |
| R1031 |
T1708 |
T1707 |
auxpass |
were,cultured |
| R1032 |
T1709 |
T1707 |
prep |
at,cultured |
| R1033 |
T1710 |
T1711 |
nummod |
27,°C |
| R1034 |
T1711 |
T1709 |
pobj |
°C,at |
| R1035 |
T1712 |
T1707 |
prep |
in,cultured |
| R1036 |
T1713 |
T1714 |
nmod |
Sf,SFM |
| R1037 |
T1714 |
T1712 |
pobj |
SFM,in |
| R1038 |
T1715 |
T1713 |
punct |
-,Sf |
| R1039 |
T1716 |
T1713 |
nummod |
900,Sf |
| R1040 |
T1717 |
T1714 |
nummod |
II,SFM |
| R1041 |
T1718 |
T1719 |
punct |
(,Inc. |
| R1042 |
T1719 |
T1714 |
parataxis |
Inc.,SFM |
| R1043 |
T1720 |
T1719 |
nmod |
Life,Inc. |
| R1044 |
T1721 |
T1719 |
nmod |
Technologies,Inc. |
| R1045 |
T1722 |
T1719 |
punct |
", ",Inc. |
| R1046 |
T1723 |
T1719 |
punct |
),Inc. |
| R1047 |
T1724 |
T1714 |
cc |
and,SFM |
| R1048 |
T1725 |
T1726 |
nmod |
EX,CELL |
| R1049 |
T1726 |
T1728 |
nmod |
CELL,medium |
| R1050 |
T1727 |
T1726 |
punct |
-,CELL |
| R1051 |
T1728 |
T1714 |
conj |
medium,SFM |
| R1052 |
T1729 |
T1726 |
nummod |
405,CELL |
| R1053 |
T1730 |
T1731 |
punct |
(,Biosciences |
| R1054 |
T1731 |
T1728 |
parataxis |
Biosciences,medium |
| R1055 |
T1732 |
T1731 |
compound |
JRH,Biosciences |
| R1056 |
T1733 |
T1731 |
punct |
),Biosciences |
| R1057 |
T1734 |
T1707 |
punct |
", ",cultured |
| R1058 |
T1735 |
T1707 |
advmod |
respectively,cultured |
| R1059 |
T1736 |
T1707 |
punct |
.,cultured |
| R1060 |
T1738 |
T1739 |
prep |
For,coinfected |
| R1061 |
T1740 |
T1738 |
pobj |
expression,For |
| R1062 |
T1741 |
T1740 |
prep |
of,expression |
| R1063 |
T1742 |
T1743 |
det |
the,proteins |
| R1064 |
T1743 |
T1741 |
pobj |
proteins,of |
| R1065 |
T1744 |
T1743 |
nmod |
Mcm4,proteins |
| R1066 |
T1745 |
T1744 |
punct |
/,Mcm4 |
| R1067 |
T1746 |
T1744 |
nummod |
6,Mcm4 |
| R1068 |
T1747 |
T1744 |
punct |
/,Mcm4 |
| R1069 |
T1748 |
T1744 |
nummod |
7,Mcm4 |
| R1070 |
T1749 |
T1739 |
punct |
", ",coinfected |
| R1071 |
T1750 |
T1751 |
amod |
High,five |
| R1072 |
T1751 |
T1752 |
nummod |
five,cells |
| R1073 |
T1752 |
T1739 |
nsubjpass |
cells,coinfected |
| R1074 |
T1753 |
T1739 |
auxpass |
were,coinfected |
| R1075 |
T1754 |
T1739 |
prep |
with,coinfected |
| R1076 |
T1755 |
T1756 |
amod |
recombinant,baculoviruses |
| R1077 |
T1756 |
T1754 |
pobj |
baculoviruses,with |
| R1078 |
T1757 |
T1756 |
acl |
expressing,baculoviruses |
| R1079 |
T1758 |
T1759 |
det |
the,proteins |
| R1080 |
T1759 |
T1757 |
dobj |
proteins,expressing |
| R1081 |
T1760 |
T1761 |
compound |
His6,Mcm6 |
| R1082 |
T1761 |
T1759 |
compound |
Mcm6,proteins |
| R1083 |
T1762 |
T1761 |
punct |
-,Mcm6 |
| R1084 |
T1763 |
T1761 |
compound |
Mcm4,Mcm6 |
| R1085 |
T1764 |
T1761 |
punct |
/,Mcm6 |
| R1086 |
T1765 |
T1756 |
cc |
and,baculoviruses |
| R1087 |
T1766 |
T1756 |
conj |
those,baculoviruses |
| R1088 |
T1767 |
T1766 |
acl |
expressing,those |
| R1089 |
T1768 |
T1769 |
det |
the,FLAG |
| R1090 |
T1769 |
T1767 |
dobj |
FLAG,expressing |
| R1091 |
T1770 |
T1769 |
compound |
Mcm7,FLAG |
| R1092 |
T1771 |
T1769 |
punct |
-,FLAG |
| R1093 |
T1772 |
T1739 |
punct |
", ",coinfected |
| R1094 |
T1773 |
T1739 |
cc |
and,coinfected |
| R1095 |
T1774 |
T1775 |
auxpass |
were,collected |
| R1096 |
T1775 |
T1739 |
conj |
collected,coinfected |
| R1097 |
T1776 |
T1775 |
prep |
at,collected |
| R1098 |
T1777 |
T1778 |
nummod |
48,h |
| R1099 |
T1778 |
T1776 |
pobj |
h,at |
| R1100 |
T1779 |
T1778 |
advmod |
post-infection,h |
| R1101 |
T1780 |
T1739 |
punct |
.,coinfected |
| R1102 |
T1782 |
T1783 |
det |
The,complexes |
| R1103 |
T1783 |
T1790 |
nsubjpass |
complexes,purified |
| R1104 |
T1784 |
T1783 |
amod |
recombinant,complexes |
| R1105 |
T1785 |
T1783 |
nmod |
Mcm4,complexes |
| R1106 |
T1786 |
T1785 |
punct |
/,Mcm4 |
| R1107 |
T1787 |
T1785 |
nummod |
6,Mcm4 |
| R1108 |
T1788 |
T1785 |
punct |
/,Mcm4 |
| R1109 |
T1789 |
T1785 |
nummod |
7,Mcm4 |
| R1110 |
T1791 |
T1790 |
auxpass |
were,purified |
| R1111 |
T1792 |
T1793 |
mark |
as,described |
| R1112 |
T1793 |
T1790 |
advcl |
described,purified |
| R1113 |
T1794 |
T1793 |
advmod |
previously,described |
| R1114 |
T1795 |
T1796 |
punct |
(,12 |
| R1115 |
T1796 |
T1793 |
parataxis |
12,described |
| R1116 |
T1797 |
T1796 |
punct |
),12 |
| R1117 |
T1798 |
T1790 |
punct |
.,purified |
| R995 |
T1668 |
T1667 |
cc |
and,Expression |
| R996 |
T1669 |
T1667 |
conj |
purification,Expression |
| R997 |
T1670 |
T1667 |
prep |
of,Expression |
| R998 |
T1671 |
T1672 |
nmod |
mouse,complex |
| R999 |
T1672 |
T1670 |
pobj |
complex,of |
