| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1448 |
0-8 |
NNS |
denotes |
Reagents |
| T1449 |
8-96 |
sentence |
denotes |
Labeled and unlabeled dNTPs/rNTPs and Sequenase were purchased from Amersham Pharmacia. |
| T1450 |
9-16 |
VBN |
denotes |
Labeled |
| T1452 |
17-20 |
CC |
denotes |
and |
| T1453 |
21-30 |
JJ |
denotes |
unlabeled |
| T1454 |
31-36 |
NNS |
denotes |
dNTPs |
| T1455 |
36-37 |
HYPH |
denotes |
/ |
| T1451 |
37-42 |
NNS |
denotes |
rNTPs |
| T1457 |
43-46 |
CC |
denotes |
and |
| T1458 |
47-56 |
NN |
denotes |
Sequenase |
| T1459 |
57-61 |
VBD |
denotes |
were |
| T1456 |
62-71 |
VBN |
denotes |
purchased |
| T1460 |
72-76 |
IN |
denotes |
from |
| T1461 |
77-85 |
NNP |
denotes |
Amersham |
| T1462 |
86-95 |
NNP |
denotes |
Pharmacia |
| T1463 |
95-96 |
. |
denotes |
. |
| T1464 |
96-323 |
sentence |
denotes |
M13mp18 and M13mp19 single-stranded circular DNA, T4 polynucleotide kinase, DNase I and the Klenow fragment were from TAKARA, Nuclease P1 was from Roche, and anti-FLAG M2 Ab (antibody)-agarose and FLAG peptide were from Sigma. |
| T1465 |
97-104 |
NN |
denotes |
M13mp18 |
| T1467 |
105-108 |
CC |
denotes |
and |
| T1468 |
109-116 |
NN |
denotes |
M13mp19 |
| T1469 |
117-123 |
JJ |
denotes |
single |
| T1471 |
123-124 |
HYPH |
denotes |
- |
| T1470 |
124-132 |
VBN |
denotes |
stranded |
| T1472 |
133-141 |
JJ |
denotes |
circular |
| T1466 |
142-145 |
NN |
denotes |
DNA |
| T1474 |
145-147 |
, |
denotes |
, |
| T1475 |
147-149 |
NN |
denotes |
T4 |
| T1477 |
150-164 |
NN |
denotes |
polynucleotide |
| T1476 |
165-171 |
NN |
denotes |
kinase |
| T1478 |
171-173 |
, |
denotes |
, |
| T1479 |
173-178 |
NN |
denotes |
DNase |
| T1480 |
179-180 |
CD |
denotes |
I |
| T1481 |
181-184 |
CC |
denotes |
and |
| T1482 |
185-188 |
DT |
denotes |
the |
| T1484 |
189-195 |
NN |
denotes |
Klenow |
| T1483 |
196-204 |
NN |
denotes |
fragment |
| T1473 |
205-209 |
VBD |
denotes |
were |
| T1485 |
210-214 |
IN |
denotes |
from |
| T1486 |
215-221 |
NN |
denotes |
TAKARA |
| T1487 |
221-223 |
, |
denotes |
, |
| T1488 |
223-231 |
NN |
denotes |
Nuclease |
| T1489 |
232-234 |
NN |
denotes |
P1 |
| T1490 |
235-238 |
VBD |
denotes |
was |
| T1491 |
239-243 |
IN |
denotes |
from |
| T1492 |
244-249 |
NNP |
denotes |
Roche |
| T1493 |
249-251 |
, |
denotes |
, |
| T1494 |
251-254 |
CC |
denotes |
and |
| T1495 |
255-264 |
JJ |
denotes |
anti-FLAG |
| T1497 |
265-267 |
NN |
denotes |
M2 |
| T1498 |
268-270 |
NN |
denotes |
Ab |
| T1500 |
271-272 |
-LRB- |
denotes |
( |
| T1499 |
272-280 |
NN |
denotes |
antibody |
| T1501 |
280-281 |
-RRB- |
denotes |
) |
| T1502 |
281-282 |
HYPH |
denotes |
- |
| T1496 |
282-289 |
NN |
denotes |
agarose |
| T1504 |
290-293 |
CC |
denotes |
and |
| T1505 |
294-298 |
NN |
denotes |
FLAG |
| T1506 |
299-306 |
NN |
denotes |
peptide |
| T1503 |
307-311 |
VBD |
denotes |
were |
| T1507 |
312-316 |
IN |
denotes |
from |
| T1508 |
317-322 |
NNP |
denotes |
Sigma |
| T1509 |
322-323 |
. |
denotes |
. |
| T1510 |
323-408 |
sentence |
denotes |
SV40 T-antigen was purified from baculovirus-infected insect cells as reported (22). |
| T1511 |
324-328 |
NN |
denotes |
SV40 |
| T1513 |
329-330 |
NN |
denotes |
T |
| T1514 |
330-331 |
HYPH |
denotes |
- |
| T1512 |
331-338 |
NN |
denotes |
antigen |
| T1516 |
339-342 |
VBD |
denotes |
was |
| T1515 |
343-351 |
VBN |
denotes |
purified |
| T1517 |
352-356 |
IN |
denotes |
from |
| T1518 |
357-368 |
NN |
denotes |
baculovirus |
| T1520 |
368-369 |
HYPH |
denotes |
- |
| T1519 |
369-377 |
VBN |
denotes |
infected |
| T1522 |
378-384 |
NN |
denotes |
insect |
| T1521 |
385-390 |
NNS |
denotes |
cells |
| T1523 |
391-393 |
IN |
denotes |
as |
| T1524 |
394-402 |
VBN |
denotes |
reported |
| T1525 |
403-404 |
-LRB- |
denotes |
( |
| T1526 |
404-406 |
CD |
denotes |
22 |
| T1527 |
406-407 |
-RRB- |
denotes |
) |
| T1528 |
407-408 |
. |
denotes |
. |
| T1529 |
408-467 |
sentence |
denotes |
PriA helicase protein was provided by Dr Taku Tanaka (23). |
| T1530 |
409-413 |
NN |
denotes |
PriA |
| T1531 |
414-422 |
NN |
denotes |
helicase |
| T1532 |
423-430 |
NN |
denotes |
protein |
| T1534 |
431-434 |
VBD |
denotes |
was |
| T1533 |
435-443 |
VBN |
denotes |
provided |
| T1535 |
444-446 |
IN |
denotes |
by |
| T1536 |
447-449 |
NNP |
denotes |
Dr |
| T1538 |
450-454 |
NNP |
denotes |
Taku |
| T1537 |
455-461 |
NNP |
denotes |
Tanaka |
| T1539 |
462-463 |
-LRB- |
denotes |
( |
| T1540 |
463-465 |
CD |
denotes |
23 |
| T1541 |
465-466 |
-RRB- |
denotes |
) |
| T1542 |
466-467 |
. |
denotes |
. |
| T1543 |
467-669 |
sentence |
denotes |
Oligonucleotides were purchased from Hokkaido system science Co., Ltd (Hokkaido, Japan), and were purified on polyacrylamide gel, followed by purification by Sep-Pak Plus C18 cartridge column (Waters). |
| T1544 |
468-484 |
NNS |
denotes |
Oligonucleotides |
| T1546 |
485-489 |
VBD |
denotes |
were |
| T1545 |
490-499 |
VBN |
denotes |
purchased |
| T1547 |
500-504 |
IN |
denotes |
from |
| T1548 |
505-513 |
NNP |
denotes |
Hokkaido |
| T1550 |
514-520 |
NNP |
denotes |
system |
| T1551 |
521-528 |
NNP |
denotes |
science |
| T1552 |
529-532 |
NNP |
denotes |
Co. |
| T1553 |
532-534 |
, |
denotes |
, |
| T1549 |
534-537 |
NNP |
denotes |
Ltd |
| T1554 |
538-539 |
-LRB- |
denotes |
( |
| T1555 |
539-547 |
NNP |
denotes |
Hokkaido |
| T1556 |
547-549 |
, |
denotes |
, |
| T1557 |
549-554 |
NNP |
denotes |
Japan |
| T1558 |
554-555 |
-RRB- |
denotes |
) |
| T1559 |
555-557 |
, |
denotes |
, |
| T1560 |
557-560 |
CC |
denotes |
and |
| T1561 |
561-565 |
VBD |
denotes |
were |
| T1562 |
566-574 |
VBN |
denotes |
purified |
| T1563 |
575-577 |
IN |
denotes |
on |
| T1564 |
578-592 |
NN |
denotes |
polyacrylamide |
| T1565 |
593-596 |
NN |
denotes |
gel |
| T1566 |
596-598 |
, |
denotes |
, |
| T1567 |
598-606 |
VBN |
denotes |
followed |
| T1568 |
607-609 |
IN |
denotes |
by |
| T1569 |
610-622 |
NN |
denotes |
purification |
| T1570 |
623-625 |
IN |
denotes |
by |
| T1571 |
626-629 |
NN |
denotes |
Sep |
| T1573 |
629-630 |
HYPH |
denotes |
- |
| T1574 |
630-633 |
NN |
denotes |
Pak |
| T1572 |
634-638 |
NN |
denotes |
Plus |
| T1576 |
639-642 |
NN |
denotes |
C18 |
| T1575 |
643-652 |
NN |
denotes |
cartridge |
| T1577 |
653-659 |
NN |
denotes |
column |
| T1578 |
660-661 |
-LRB- |
denotes |
( |
| T1579 |
661-667 |
NNP |
denotes |
Waters |
| T1580 |
667-668 |
-RRB- |
denotes |
) |
| T1581 |
668-669 |
. |
denotes |
. |
| R872 |
T1450 |
T1451 |
amod |
Labeled,rNTPs |
| R873 |
T1451 |
T1456 |
nsubjpass |
rNTPs,purchased |
| R874 |
T1452 |
T1450 |
cc |
and,Labeled |
| R875 |
T1453 |
T1450 |
conj |
unlabeled,Labeled |
| R876 |
T1454 |
T1451 |
compound |
dNTPs,rNTPs |
| R877 |
T1455 |
T1451 |
punct |
/,rNTPs |
| R878 |
T1457 |
T1451 |
cc |
and,rNTPs |
| R879 |
T1458 |
T1451 |
conj |
Sequenase,rNTPs |
| R880 |
T1459 |
T1456 |
auxpass |
were,purchased |
| R881 |
T1460 |
T1456 |
prep |
from,purchased |
| R882 |
T1461 |
T1462 |
compound |
Amersham,Pharmacia |
| R883 |
T1462 |
T1460 |
pobj |
Pharmacia,from |
| R884 |
T1463 |
T1456 |
punct |
.,purchased |
| R885 |
T1465 |
T1466 |
nmod |
M13mp18,DNA |
| R886 |
T1466 |
T1473 |
nsubj |
DNA,were |
| R887 |
T1467 |
T1465 |
cc |
and,M13mp18 |
| R888 |
T1468 |
T1465 |
conj |
M13mp19,M13mp18 |
| R889 |
T1469 |
T1470 |
amod |
single,stranded |
| R890 |
T1470 |
T1466 |
amod |
stranded,DNA |
| R891 |
T1471 |
T1470 |
punct |
-,stranded |
| R892 |
T1472 |
T1466 |
amod |
circular,DNA |
| R893 |
T1474 |
T1466 |
punct |
", ",DNA |
| R894 |
T1475 |
T1476 |
compound |
T4,kinase |
| R895 |
T1476 |
T1466 |
conj |
kinase,DNA |
| R896 |
T1477 |
T1476 |
compound |
polynucleotide,kinase |
| R897 |
T1478 |
T1476 |
punct |
", ",kinase |
| R898 |
T1479 |
T1476 |
conj |
DNase,kinase |
| R899 |
T1480 |
T1479 |
nummod |
I,DNase |
| R900 |
T1481 |
T1479 |
cc |
and,DNase |
| R901 |
T1482 |
T1483 |
det |
the,fragment |
| R902 |
T1483 |
T1479 |
conj |
fragment,DNase |
| R903 |
T1484 |
T1483 |
compound |
Klenow,fragment |
| R904 |
T1485 |
T1473 |
prep |
from,were |
| R905 |
T1486 |
T1485 |
pobj |
TAKARA,from |
| R906 |
T1487 |
T1473 |
punct |
", ",were |
| R907 |
T1488 |
T1489 |
compound |
Nuclease,P1 |
| R908 |
T1489 |
T1490 |
nsubj |
P1,was |
| R909 |
T1490 |
T1473 |
conj |
was,were |
| R910 |
T1491 |
T1490 |
prep |
from,was |
| R911 |
T1492 |
T1491 |
pobj |
Roche,from |
| R912 |
T1493 |
T1490 |
punct |
", ",was |
| R913 |
T1494 |
T1490 |
cc |
and,was |
| R914 |
T1495 |
T1496 |
amod |
anti-FLAG,agarose |
| R915 |
T1496 |
T1503 |
nsubj |
agarose,were |
| R916 |
T1497 |
T1496 |
nmod |
M2,agarose |
| R917 |
T1498 |
T1499 |
nmod |
Ab,antibody |
| R918 |
T1499 |
T1496 |
nmod |
antibody,agarose |
| R919 |
T1500 |
T1499 |
punct |
(,antibody |
| R920 |
T1501 |
T1496 |
punct |
),agarose |
| R921 |
T1502 |
T1496 |
punct |
-,agarose |
| R922 |
T1503 |
T1490 |
conj |
were,was |
| R923 |
T1504 |
T1496 |
cc |
and,agarose |
| R924 |
T1505 |
T1506 |
compound |
FLAG,peptide |
| R925 |
T1506 |
T1496 |
conj |
peptide,agarose |
| R926 |
T1507 |
T1503 |
prep |
from,were |
| R927 |
T1508 |
T1507 |
pobj |
Sigma,from |
| R928 |
T1509 |
T1503 |
punct |
.,were |
| R929 |
T1511 |
T1512 |
compound |
SV40,antigen |
| R930 |
T1512 |
T1515 |
nsubjpass |
antigen,purified |
| R931 |
T1513 |
T1512 |
compound |
T,antigen |
| R932 |
T1514 |
T1512 |
punct |
-,antigen |
| R933 |
T1516 |
T1515 |
auxpass |
was,purified |
| R934 |
T1517 |
T1515 |
prep |
from,purified |
| R935 |
T1518 |
T1519 |
npadvmod |
baculovirus,infected |
| R936 |
T1519 |
T1521 |
amod |
infected,cells |
| R937 |
T1520 |
T1519 |
punct |
-,infected |
| R938 |
T1521 |
T1517 |
pobj |
cells,from |
| R939 |
T1522 |
T1521 |
compound |
insect,cells |
| R940 |
T1523 |
T1524 |
mark |
as,reported |
| R941 |
T1524 |
T1515 |
advcl |
reported,purified |
| R942 |
T1525 |
T1526 |
punct |
(,22 |
| R943 |
T1526 |
T1524 |
parataxis |
22,reported |
| R944 |
T1527 |
T1526 |
punct |
),22 |
| R945 |
T1528 |
T1515 |
punct |
.,purified |
| R946 |
T1530 |
T1531 |
compound |
PriA,helicase |
| R947 |
T1531 |
T1532 |
compound |
helicase,protein |
| R948 |
T1532 |
T1533 |
nsubjpass |
protein,provided |
| R949 |
T1534 |
T1533 |
auxpass |
was,provided |
| R950 |
T1535 |
T1533 |
agent |
by,provided |
| R951 |
T1536 |
T1537 |
compound |
Dr,Tanaka |
| R952 |
T1537 |
T1535 |
pobj |
Tanaka,by |
| R953 |
T1538 |
T1537 |
compound |
Taku,Tanaka |
| R954 |
T1539 |
T1540 |
punct |
(,23 |
| R955 |
T1540 |
T1533 |
parataxis |
23,provided |
| R956 |
T1541 |
T1540 |
punct |
),23 |
| R957 |
T1542 |
T1533 |
punct |
.,provided |
| R958 |
T1544 |
T1545 |
nsubjpass |
Oligonucleotides,purchased |
| R959 |
T1546 |
T1545 |
auxpass |
were,purchased |
| R960 |
T1547 |
T1545 |
prep |
from,purchased |
| R961 |
T1548 |
T1549 |
nmod |
Hokkaido,Ltd |
| R962 |
T1549 |
T1547 |
pobj |
Ltd,from |
| R963 |
T1550 |
T1549 |
nmod |
system,Ltd |
| R964 |
T1551 |
T1549 |
nmod |
science,Ltd |
| R965 |
T1552 |
T1549 |
nmod |
Co.,Ltd |
| R966 |
T1553 |
T1549 |
punct |
", ",Ltd |
| R967 |
T1554 |
T1555 |
punct |
(,Hokkaido |
| R968 |
T1555 |
T1549 |
parataxis |
Hokkaido,Ltd |
| R969 |
T1556 |
T1555 |
punct |
", ",Hokkaido |
| R970 |
T1557 |
T1555 |
npadvmod |
Japan,Hokkaido |
| R971 |
T1558 |
T1555 |
punct |
),Hokkaido |
| R972 |
T1559 |
T1545 |
punct |
", ",purchased |
| R973 |
T1560 |
T1545 |
cc |
and,purchased |
| R974 |
T1561 |
T1562 |
auxpass |
were,purified |
| R975 |
T1562 |
T1545 |
conj |
purified,purchased |
| R976 |
T1563 |
T1562 |
prep |
on,purified |
| R977 |
T1564 |
T1565 |
compound |
polyacrylamide,gel |
| R978 |
T1565 |
T1563 |
pobj |
gel,on |
| R979 |
T1566 |
T1562 |
punct |
", ",purified |
| R980 |
T1567 |
T1562 |
advcl |
followed,purified |
| R981 |
T1568 |
T1567 |
agent |
by,followed |
| R982 |
T1569 |
T1568 |
pobj |
purification,by |
| R983 |
T1570 |
T1569 |
prep |
by,purification |
| R984 |
T1571 |
T1572 |
compound |
Sep,Plus |
| R985 |
T1572 |
T1575 |
compound |
Plus,cartridge |
| R986 |
T1573 |
T1572 |
punct |
-,Plus |
| R987 |
T1574 |
T1572 |
compound |
Pak,Plus |
| R988 |
T1575 |
T1577 |
compound |
cartridge,column |
| R989 |
T1576 |
T1575 |
compound |
C18,cartridge |
| R990 |
T1577 |
T1570 |
pobj |
column,by |
| R991 |
T1578 |
T1579 |
punct |
(,Waters |
| R992 |
T1579 |
T1577 |
parataxis |
Waters,column |
| R993 |
T1580 |
T1579 |
punct |
),Waters |
| R994 |
T1581 |
T1545 |
punct |
.,purchased |
