PMC:1140370 / 28060-37852 JSON TXT 6 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T8790	11-14	NN	denotes	DNA
T8791	15-23	NN	denotes	helicase
T8792	24-26	VBZ	denotes	is
T8793	27-28	DT	denotes	a
T8795	29-36	JJ	denotes	central
T8794	37-43	NN	denotes	factor
T8796	44-47	IN	denotes	for
T8797	48-57	NN	denotes	operation
T8798	58-60	IN	denotes	of
T8799	61-72	NN	denotes	replication
T8800	73-78	NNS	denotes	forks
T8801	78-79	.	denotes	.
T8802	79-159	sentence	denotes	It also plays a crucial role in the initiation step at the replication origins.
T8803	80-82	PRP	denotes	It
T8805	83-87	RB	denotes	also
T8804	88-93	VBZ	denotes	plays
T8806	94-95	DT	denotes	a
T8808	96-103	JJ	denotes	crucial
T8807	104-108	NN	denotes	role
T8809	109-111	IN	denotes	in
T8810	112-115	DT	denotes	the
T8812	116-126	NN	denotes	initiation
T8811	127-131	NN	denotes	step
T8813	132-134	IN	denotes	at
T8814	135-138	DT	denotes	the
T8816	139-150	NN	denotes	replication
T8815	151-158	NNS	denotes	origins
T8817	158-159	.	denotes	.
T8818	159-357	sentence	denotes	Therefore, the elucidation of how the replicative helicases interact with DNA substrate and how they are activated is crucial for understanding the molecular basis of initiation of DNA replication.
T8819	160-169	RB	denotes	Therefore
T8821	169-171	,	denotes	,
T8822	171-174	DT	denotes	the
T8823	175-186	NN	denotes	elucidation
T8824	187-189	IN	denotes	of
T8825	190-193	WRB	denotes	how
T8827	194-197	DT	denotes	the
T8829	198-209	JJ	denotes	replicative
T8828	210-219	NNS	denotes	helicases
T8826	220-228	VBP	denotes	interact
T8830	229-233	IN	denotes	with
T8831	234-237	NN	denotes	DNA
T8832	238-247	NN	denotes	substrate
T8833	248-251	CC	denotes	and
T8834	252-255	WRB	denotes	how
T8836	256-260	PRP	denotes	they
T8837	261-264	VBP	denotes	are
T8835	265-274	VBN	denotes	activated
T8820	275-277	VBZ	denotes	is
T8838	278-285	JJ	denotes	crucial
T8839	286-289	IN	denotes	for
T8840	290-303	VBG	denotes	understanding
T8841	304-307	DT	denotes	the
T8843	308-317	JJ	denotes	molecular
T8842	318-323	NN	denotes	basis
T8844	324-326	IN	denotes	of
T8845	327-337	NN	denotes	initiation
T8846	338-340	IN	denotes	of
T8847	341-344	NN	denotes	DNA
T8848	345-356	NN	denotes	replication
T8849	356-357	.	denotes	.
T8850	357-446	sentence	denotes	It is now believed that Mcm is a major component of the eukaryotic replicative helicase.
T8851	358-360	PRP	denotes	It
T8853	361-363	VBZ	denotes	is
T8854	364-367	RB	denotes	now
T8852	368-376	VBN	denotes	believed
T8855	377-381	IN	denotes	that
T8857	382-385	NN	denotes	Mcm
T8856	386-388	VBZ	denotes	is
T8858	389-390	DT	denotes	a
T8860	391-396	JJ	denotes	major
T8859	397-406	NN	denotes	component
T8861	407-409	IN	denotes	of
T8862	410-413	DT	denotes	the
T8864	414-424	JJ	denotes	eukaryotic
T8865	425-436	JJ	denotes	replicative
T8863	437-445	NN	denotes	helicase
T8866	445-446	.	denotes	.
T8867	446-611	sentence	denotes	In this report, we have conducted detailed analyses on binding and helicase actions of mouse Mcm4/6/7 using various substrates including fork and bubble structures.
T8868	447-449	IN	denotes	In
T8870	450-454	DT	denotes	this
T8871	455-461	NN	denotes	report
T8872	461-463	,	denotes	,
T8873	463-465	PRP	denotes	we
T8874	466-470	VBP	denotes	have
T8869	471-480	VBN	denotes	conducted
T8875	481-489	JJ	denotes	detailed
T8876	490-498	NNS	denotes	analyses
T8877	499-501	IN	denotes	on
T8878	502-509	NN	denotes	binding
T8880	510-513	CC	denotes	and
T8881	514-522	NN	denotes	helicase
T8879	523-530	NNS	denotes	actions
T8882	531-533	IN	denotes	of
T8883	534-539	NN	denotes	mouse
T8884	540-544	NN	denotes	Mcm4
T8885	544-545	HYPH	denotes	/
T8886	545-546	CD	denotes	6
T8887	546-547	HYPH	denotes	/
T8888	547-548	CD	denotes	7
T8889	549-554	VBG	denotes	using
T8890	555-562	JJ	denotes	various
T8891	563-573	NNS	denotes	substrates
T8892	574-583	VBG	denotes	including
T8893	584-588	NN	denotes	fork
T8895	589-592	CC	denotes	and
T8896	593-599	NN	denotes	bubble
T8894	600-610	NNS	denotes	structures
T8897	610-611	.	denotes	.
T9088	613-618	NNS	denotes	Modes
T9089	619-621	IN	denotes	of
T9090	622-629	NN	denotes	binding
T9091	630-633	CC	denotes	and
T9092	634-644	NN	denotes	activation
T9093	645-647	IN	denotes	of
T9094	648-652	NN	denotes	Mcm4
T9096	652-653	HYPH	denotes	/
T9097	653-654	CD	denotes	6
T9098	654-655	HYPH	denotes	/
T9099	655-656	CD	denotes	7
T9095	657-665	NN	denotes	helicase
T9100	666-668	IN	denotes	on
T9101	669-670	DT	denotes	a
T9103	671-677	NN	denotes	bubble
T9102	678-687	NN	denotes	structure
T9104	687-794	sentence	denotes	Initiation of DNA replication is associated with localized melting of duplex DNA near replication origins.
T9105	688-698	NN	denotes	Initiation
T9107	699-701	IN	denotes	of
T9108	702-705	NN	denotes	DNA
T9109	706-717	NN	denotes	replication
T9110	718-720	VBZ	denotes	is
T9106	721-731	VBN	denotes	associated
T9111	732-736	IN	denotes	with
T9112	737-746	VBN	denotes	localized
T9113	747-754	NN	denotes	melting
T9114	755-757	IN	denotes	of
T9115	758-764	NN	denotes	duplex
T9116	765-768	NN	denotes	DNA
T9117	769-773	JJ	denotes	near
T9118	774-785	NN	denotes	replication
T9119	786-793	NNS	denotes	origins
T9120	793-794	.	denotes	.
T9121	794-913	sentence	denotes	Helicases are loaded onto replication forks through the melted region, induced by initiator binding, in bacteria (32).
T9122	795-804	NNS	denotes	Helicases
T9124	805-808	VBP	denotes	are
T9123	809-815	VBN	denotes	loaded
T9125	816-820	IN	denotes	onto
T9126	821-832	NN	denotes	replication
T9127	833-838	NNS	denotes	forks
T9128	839-846	IN	denotes	through
T9129	847-850	DT	denotes	the
T9131	851-857	VBN	denotes	melted
T9130	858-864	NN	denotes	region
T9132	864-866	,	denotes	,
T9133	866-873	VBN	denotes	induced
T9134	874-876	IN	denotes	by
T9135	877-886	NN	denotes	initiator
T9136	887-894	NN	denotes	binding
T9137	894-896	,	denotes	,
T9138	896-898	IN	denotes	in
T9139	899-907	NNS	denotes	bacteria
T9140	908-909	-LRB-	denotes	(
T9141	909-911	CD	denotes	32
T9142	911-912	-RRB-	denotes	)
T9143	912-913	.	denotes	.
T9144	913-1044	sentence	denotes	We previously reported that Mcm4/6/7 can be loaded onto a bubble-like structure and can serve as a DNA helicase at the forks (13).
T9145	914-916	PRP	denotes	We
T9147	917-927	RB	denotes	previously
T9146	928-936	VBD	denotes	reported
T9148	937-941	IN	denotes	that
T9150	942-946	NN	denotes	Mcm4
T9151	946-947	HYPH	denotes	/
T9152	947-948	CD	denotes	6
T9153	948-949	HYPH	denotes	/
T9154	949-950	CD	denotes	7
T9155	951-954	MD	denotes	can
T9156	955-957	VB	denotes	be
T9149	958-964	VBN	denotes	loaded
T9157	965-969	IN	denotes	onto
T9158	970-971	DT	denotes	a
T9160	972-978	NN	denotes	bubble
T9162	978-979	HYPH	denotes	-
T9161	979-983	JJ	denotes	like
T9159	984-993	NN	denotes	structure
T9163	994-997	CC	denotes	and
T9164	998-1001	MD	denotes	can
T9165	1002-1007	VB	denotes	serve
T9166	1008-1010	IN	denotes	as
T9167	1011-1012	DT	denotes	a
T9169	1013-1016	NN	denotes	DNA
T9168	1017-1025	NN	denotes	helicase
T9170	1026-1028	IN	denotes	at
T9171	1029-1032	DT	denotes	the
T9172	1033-1038	NNS	denotes	forks
T9173	1039-1040	-LRB-	denotes	(
T9174	1040-1042	CD	denotes	13
T9175	1042-1043	-RRB-	denotes	)
T9176	1043-1044	.	denotes	.
T9177	1044-1238	sentence	denotes	The ability of Mcm4/6/7 to unwind the bubble substrate but not the conventional duplex DNA (Figures 2 and 13) indicates that Mcm can be loaded through the single-stranded segment of the bubble.
T9178	1045-1048	DT	denotes	The
T9179	1049-1056	NN	denotes	ability
T9181	1057-1059	IN	denotes	of
T9182	1060-1064	NN	denotes	Mcm4
T9183	1064-1065	HYPH	denotes	/
T9184	1065-1066	CD	denotes	6
T9185	1066-1067	HYPH	denotes	/
T9186	1067-1068	CD	denotes	7
T9187	1069-1071	TO	denotes	to
T9188	1072-1078	VB	denotes	unwind
T9189	1079-1082	DT	denotes	the
T9191	1083-1089	NN	denotes	bubble
T9190	1090-1099	NN	denotes	substrate
T9192	1100-1103	CC	denotes	but
T9193	1104-1107	RB	denotes	not
T9194	1108-1111	DT	denotes	the
T9196	1112-1124	JJ	denotes	conventional
T9197	1125-1131	NN	denotes	duplex
T9195	1132-1135	NN	denotes	DNA
T9198	1136-1137	-LRB-	denotes	(
T9200	1137-1144	NNS	denotes	Figures
T9199	1145-1146	CD	denotes	2
T9201	1147-1150	CC	denotes	and
T9202	1151-1153	CD	denotes	13
T9203	1153-1154	-RRB-	denotes	)
T9180	1155-1164	VBZ	denotes	indicates
T9204	1165-1169	IN	denotes	that
T9206	1170-1173	NN	denotes	Mcm
T9207	1174-1177	MD	denotes	can
T9208	1178-1180	VB	denotes	be
T9205	1181-1187	VBN	denotes	loaded
T9209	1188-1195	IN	denotes	through
T9210	1196-1199	DT	denotes	the
T9212	1200-1206	JJ	denotes	single
T9214	1206-1207	HYPH	denotes	-
T9213	1207-1215	VBN	denotes	stranded
T9211	1216-1223	NN	denotes	segment
T9215	1224-1226	IN	denotes	of
T9216	1227-1230	DT	denotes	the
T9217	1231-1237	NN	denotes	bubble
T9218	1237-1238	.	denotes	.
T9219	1238-1391	sentence	denotes	Furthermore, the Mcm4/6/7 complex displays marked preference for thymine-rich sequences within the single-stranded segment for helicase activation (13).
T9220	1239-1250	RB	denotes	Furthermore
T9222	1250-1252	,	denotes	,
T9223	1252-1255	DT	denotes	the
T9225	1256-1260	NN	denotes	Mcm4
T9226	1260-1261	HYPH	denotes	/
T9227	1261-1262	CD	denotes	6
T9228	1262-1263	HYPH	denotes	/
T9229	1263-1264	CD	denotes	7
T9224	1265-1272	NN	denotes	complex
T9221	1273-1281	VBZ	denotes	displays
T9230	1282-1288	JJ	denotes	marked
T9231	1289-1299	NN	denotes	preference
T9232	1300-1303	IN	denotes	for
T9233	1304-1311	NN	denotes	thymine
T9235	1311-1312	HYPH	denotes	-
T9234	1312-1316	JJ	denotes	rich
T9236	1317-1326	NNS	denotes	sequences
T9237	1327-1333	IN	denotes	within
T9238	1334-1337	DT	denotes	the
T9240	1338-1344	JJ	denotes	single
T9242	1344-1345	HYPH	denotes	-
T9241	1345-1353	VBN	denotes	stranded
T9239	1354-1361	NN	denotes	segment
T9243	1362-1365	IN	denotes	for
T9244	1366-1374	NN	denotes	helicase
T9245	1375-1385	NN	denotes	activation
T9246	1386-1387	-LRB-	denotes	(
T9247	1387-1389	CD	denotes	13
T9248	1389-1390	-RRB-	denotes	)
T9249	1390-1391	.	denotes	.
T9250	1391-1699	sentence	denotes	The results in this report indicate that helicase action of Mcm4/6/7 on synthetic bubbles may depend on the presence of an unpaired region of sufficient length (at least 40 nt), which may permit assembly of a double-hexameric complex on the substrate DNA, similar to SV40 T-antigen protein (Figure 2A) (21).
T9251	1392-1395	DT	denotes	The
T9252	1396-1403	NNS	denotes	results
T9254	1404-1406	IN	denotes	in
T9255	1407-1411	DT	denotes	this
T9256	1412-1418	NN	denotes	report
T9253	1419-1427	VBP	denotes	indicate
T9257	1428-1432	IN	denotes	that
T9259	1433-1441	NN	denotes	helicase
T9260	1442-1448	NN	denotes	action
T9261	1449-1451	IN	denotes	of
T9262	1452-1456	NN	denotes	Mcm4
T9263	1456-1457	HYPH	denotes	/
T9264	1457-1458	CD	denotes	6
T9265	1458-1459	HYPH	denotes	/
T9266	1459-1460	CD	denotes	7
T9267	1461-1463	IN	denotes	on
T9268	1464-1473	JJ	denotes	synthetic
T9269	1474-1481	NNS	denotes	bubbles
T9270	1482-1485	MD	denotes	may
T9258	1486-1492	VB	denotes	depend
T9271	1493-1495	IN	denotes	on
T9272	1496-1499	DT	denotes	the
T9273	1500-1508	NN	denotes	presence
T9274	1509-1511	IN	denotes	of
T9275	1512-1514	DT	denotes	an
T9277	1515-1523	JJ	denotes	unpaired
T9276	1524-1530	NN	denotes	region
T9278	1531-1533	IN	denotes	of
T9279	1534-1544	JJ	denotes	sufficient
T9280	1545-1551	NN	denotes	length
T9281	1552-1553	-LRB-	denotes	(
T9283	1553-1555	RB	denotes	at
T9284	1556-1561	RBS	denotes	least
T9285	1562-1564	CD	denotes	40
T9282	1565-1567	NN	denotes	nt
T9286	1567-1568	-RRB-	denotes	)
T9287	1568-1570	,	denotes	,
T9288	1570-1575	WDT	denotes	which
T9290	1576-1579	MD	denotes	may
T9289	1580-1586	VB	denotes	permit
T9291	1587-1595	NN	denotes	assembly
T9292	1596-1598	IN	denotes	of
T9293	1599-1600	DT	denotes	a
T9295	1601-1607	JJ	denotes	double
T9297	1607-1608	HYPH	denotes	-
T9296	1608-1617	JJ	denotes	hexameric
T9294	1618-1625	NN	denotes	complex
T9298	1626-1628	IN	denotes	on
T9299	1629-1632	DT	denotes	the
T9301	1633-1642	NN	denotes	substrate
T9300	1643-1646	NN	denotes	DNA
T9302	1646-1648	,	denotes	,
T9303	1648-1655	JJ	denotes	similar
T9304	1656-1658	IN	denotes	to
T9305	1659-1663	NN	denotes	SV40
T9307	1664-1665	NN	denotes	T
T9309	1665-1666	HYPH	denotes	-
T9308	1666-1673	NN	denotes	antigen
T9306	1674-1681	NN	denotes	protein
T9310	1682-1683	-LRB-	denotes	(
T9312	1683-1689	NN	denotes	Figure
T9311	1690-1692	NN	denotes	2A
T9313	1692-1693	-RRB-	denotes	)
T9314	1694-1695	-LRB-	denotes	(
T9315	1695-1697	CD	denotes	21
T9316	1697-1698	-RRB-	denotes	)
T9317	1698-1699	.	denotes	.
T9318	1699-1858	sentence	denotes	When one strand of the single-stranded segment in T-rich bubble was replaced by guanine-rich sequences, the efficiency of unwinding was significantly reduced.
T9319	1700-1704	WRB	denotes	When
T9321	1705-1708	CD	denotes	one
T9322	1709-1715	NN	denotes	strand
T9323	1716-1718	IN	denotes	of
T9324	1719-1722	DT	denotes	the
T9326	1723-1729	JJ	denotes	single
T9328	1729-1730	HYPH	denotes	-
T9327	1730-1738	VBN	denotes	stranded
T9325	1739-1746	NN	denotes	segment
T9329	1747-1749	IN	denotes	in
T9330	1750-1751	NN	denotes	T
T9332	1751-1752	HYPH	denotes	-
T9331	1752-1756	JJ	denotes	rich
T9333	1757-1763	NN	denotes	bubble
T9334	1764-1767	VBD	denotes	was
T9320	1768-1776	VBN	denotes	replaced
T9336	1777-1779	IN	denotes	by
T9337	1780-1787	NN	denotes	guanine
T9339	1787-1788	HYPH	denotes	-
T9338	1788-1792	JJ	denotes	rich
T9340	1793-1802	NNS	denotes	sequences
T9341	1802-1804	,	denotes	,
T9342	1804-1807	DT	denotes	the
T9343	1808-1818	NN	denotes	efficiency
T9344	1819-1821	IN	denotes	of
T9345	1822-1831	NN	denotes	unwinding
T9346	1832-1835	VBD	denotes	was
T9347	1836-1849	RB	denotes	significantly
T9335	1850-1857	VBN	denotes	reduced
T9348	1857-1858	.	denotes	.
T9349	1858-2042	sentence	denotes	Our footprinting analyses showed that Mcm4/6/7 strongly protects 25 nt single-stranded DNA segment adjacent to each branch point and proximal to 5′ ends of both strands of the bubble.
T9350	1859-1862	PRP$	denotes	Our
T9352	1863-1875	NN	denotes	footprinting
T9351	1876-1884	NNS	denotes	analyses
T9353	1885-1891	VBD	denotes	showed
T9354	1892-1896	IN	denotes	that
T9356	1897-1901	NN	denotes	Mcm4
T9357	1901-1902	HYPH	denotes	/
T9358	1902-1903	CD	denotes	6
T9359	1903-1904	HYPH	denotes	/
T9360	1904-1905	CD	denotes	7
T9361	1906-1914	RB	denotes	strongly
T9355	1915-1923	VBZ	denotes	protects
T9362	1924-1926	CD	denotes	25
T9363	1927-1929	NN	denotes	nt
T9365	1930-1936	JJ	denotes	single
T9367	1936-1937	HYPH	denotes	-
T9366	1937-1945	VBN	denotes	stranded
T9368	1946-1949	NN	denotes	DNA
T9364	1950-1957	NN	denotes	segment
T9369	1958-1966	JJ	denotes	adjacent
T9370	1967-1969	IN	denotes	to
T9371	1970-1974	DT	denotes	each
T9373	1975-1981	NN	denotes	branch
T9372	1982-1987	NN	denotes	point
T9374	1988-1991	CC	denotes	and
T9375	1992-2000	JJ	denotes	proximal
T9376	2001-2003	IN	denotes	to
T9377	2004-2005	CD	denotes	5
T9379	2005-2006	SYM	denotes	′
T9378	2007-2011	NNS	denotes	ends
T9380	2012-2014	IN	denotes	of
T9381	2015-2019	DT	denotes	both
T9382	2020-2027	NNS	denotes	strands
T9383	2028-2030	IN	denotes	of
T9384	2031-2034	DT	denotes	the
T9385	2035-2041	NN	denotes	bubble
T9386	2041-2042	.	denotes	.
T9387	2042-2284	sentence	denotes	At each fork, one Mcm4/6/7 complex is likely to encircle the single-stranded DNA strand and two hexamers may bind symmetrically to the bubble substrate, forming a double hexameric structure on the bubble (see schematic drawing of Figure 1A).
T9388	2043-2045	IN	denotes	At
T9390	2046-2050	DT	denotes	each
T9391	2051-2055	NN	denotes	fork
T9392	2055-2057	,	denotes	,
T9393	2057-2060	CD	denotes	one
T9395	2061-2065	NN	denotes	Mcm4
T9396	2065-2066	HYPH	denotes	/
T9397	2066-2067	CD	denotes	6
T9398	2067-2068	HYPH	denotes	/
T9399	2068-2069	CD	denotes	7
T9394	2070-2077	NN	denotes	complex
T9389	2078-2080	VBZ	denotes	is
T9400	2081-2087	JJ	denotes	likely
T9401	2088-2090	TO	denotes	to
T9402	2091-2099	VB	denotes	encircle
T9403	2100-2103	DT	denotes	the
T9405	2104-2110	JJ	denotes	single
T9407	2110-2111	HYPH	denotes	-
T9406	2111-2119	VBN	denotes	stranded
T9408	2120-2123	NN	denotes	DNA
T9404	2124-2130	NN	denotes	strand
T9409	2131-2134	CC	denotes	and
T9410	2135-2138	CD	denotes	two
T9411	2139-2147	NNS	denotes	hexamers
T9413	2148-2151	MD	denotes	may
T9412	2152-2156	VB	denotes	bind
T9414	2157-2170	RB	denotes	symmetrically
T9415	2171-2173	IN	denotes	to
T9416	2174-2177	DT	denotes	the
T9418	2178-2184	NN	denotes	bubble
T9417	2185-2194	NN	denotes	substrate
T9419	2194-2196	,	denotes	,
T9420	2196-2203	VBG	denotes	forming
T9421	2204-2205	DT	denotes	a
T9423	2206-2212	JJ	denotes	double
T9424	2213-2222	JJ	denotes	hexameric
T9422	2223-2232	NN	denotes	structure
T9425	2233-2235	IN	denotes	on
T9426	2236-2239	DT	denotes	the
T9427	2240-2246	NN	denotes	bubble
T9428	2247-2248	-LRB-	denotes	(
T9429	2248-2251	VB	denotes	see
T9430	2252-2261	JJ	denotes	schematic
T9431	2262-2269	NN	denotes	drawing
T9432	2270-2272	IN	denotes	of
T9433	2273-2279	NN	denotes	Figure
T9434	2280-2282	NN	denotes	1A
T9435	2282-2283	-RRB-	denotes	)
T9436	2283-2284	.	denotes	.
T9437	2284-2473	sentence	denotes	Efficient unwinding into both directions may require simultaneous activation of both hexamers which may sit at the center while extruding the unwound single-stranded DNA through the rings.
T9438	2285-2294	JJ	denotes	Efficient
T9439	2295-2304	NN	denotes	unwinding
T9441	2305-2309	IN	denotes	into
T9442	2310-2314	DT	denotes	both
T9443	2315-2325	NNS	denotes	directions
T9444	2326-2329	MD	denotes	may
T9440	2330-2337	VB	denotes	require
T9445	2338-2350	JJ	denotes	simultaneous
T9446	2351-2361	NN	denotes	activation
T9447	2362-2364	IN	denotes	of
T9448	2365-2369	DT	denotes	both
T9449	2370-2378	NNS	denotes	hexamers
T9450	2379-2384	WDT	denotes	which
T9452	2385-2388	MD	denotes	may
T9451	2389-2392	VB	denotes	sit
T9453	2393-2395	IN	denotes	at
T9454	2396-2399	DT	denotes	the
T9455	2400-2406	NN	denotes	center
T9456	2407-2412	IN	denotes	while
T9457	2413-2422	VBG	denotes	extruding
T9458	2423-2426	DT	denotes	the
T9460	2427-2434	JJ	denotes	unwound
T9461	2435-2441	JJ	denotes	single
T9463	2441-2442	HYPH	denotes	-
T9462	2442-2450	VBN	denotes	stranded
T9459	2451-2454	NN	denotes	DNA
T9464	2455-2462	IN	denotes	through
T9465	2463-2466	DT	denotes	the
T9466	2467-2472	NNS	denotes	rings
T9467	2472-2473	.	denotes	.
T9468	2473-2569	sentence	denotes	This may closely resemble the proposed modes of binding and helicase actions of T-antigen (21).
T9469	2474-2478	DT	denotes	This
T9471	2479-2482	MD	denotes	may
T9472	2483-2490	RB	denotes	closely
T9470	2491-2499	VB	denotes	resemble
T9473	2500-2503	DT	denotes	the
T9475	2504-2512	VBN	denotes	proposed
T9474	2513-2518	NNS	denotes	modes
T9476	2519-2521	IN	denotes	of
T9477	2522-2529	NN	denotes	binding
T9479	2530-2533	CC	denotes	and
T9480	2534-2542	NN	denotes	helicase
T9478	2543-2550	NNS	denotes	actions
T9481	2551-2553	IN	denotes	of
T9482	2554-2555	NN	denotes	T
T9484	2555-2556	HYPH	denotes	-
T9483	2556-2563	NN	denotes	antigen
T9485	2564-2565	-LRB-	denotes	(
T9486	2565-2567	CD	denotes	21
T9487	2567-2568	-RRB-	denotes	)
T9488	2568-2569	.	denotes	.
T9489	2569-2947	sentence	denotes	Although we cannot totally exclude the possibility that one Mcm4/6/7 hexamer at each fork unwinds the duplex independently, ring-shaped structures of mouse and archea MCM, as revealed by electron microscopy, bear much similarity to the recently solved structure of the SV40 large T-antigen (4,6), and are in favor of the double-hexameric structure of mouse Mcm on a bubble DNA.
T9490	2570-2578	IN	denotes	Although
T9492	2579-2581	PRP	denotes	we
T9493	2582-2585	MD	denotes	can
T9494	2585-2588	RB	denotes	not
T9495	2589-2596	RB	denotes	totally
T9491	2597-2604	VB	denotes	exclude
T9497	2605-2608	DT	denotes	the
T9498	2609-2620	NN	denotes	possibility
T9499	2621-2625	IN	denotes	that
T9501	2626-2629	CD	denotes	one
T9503	2630-2634	NN	denotes	Mcm4
T9504	2634-2635	HYPH	denotes	/
T9505	2635-2636	CD	denotes	6
T9506	2636-2637	HYPH	denotes	/
T9507	2637-2638	CD	denotes	7
T9502	2639-2646	NN	denotes	hexamer
T9508	2647-2649	IN	denotes	at
T9509	2650-2654	DT	denotes	each
T9510	2655-2659	NN	denotes	fork
T9500	2660-2667	VBZ	denotes	unwinds
T9511	2668-2671	DT	denotes	the
T9512	2672-2678	NN	denotes	duplex
T9513	2679-2692	RB	denotes	independently
T9514	2692-2694	,	denotes	,
T9515	2694-2698	NN	denotes	ring
T9517	2698-2699	HYPH	denotes	-
T9516	2699-2705	VBN	denotes	shaped
T9518	2706-2716	NNS	denotes	structures
T9519	2717-2719	IN	denotes	of
T9520	2720-2725	NN	denotes	mouse
T9522	2726-2729	CC	denotes	and
T9523	2730-2736	NN	denotes	archea
T9521	2737-2740	NN	denotes	MCM
T9524	2740-2742	,	denotes	,
T9525	2742-2744	IN	denotes	as
T9526	2745-2753	VBN	denotes	revealed
T9527	2754-2756	IN	denotes	by
T9528	2757-2765	NN	denotes	electron
T9529	2766-2776	NN	denotes	microscopy
T9530	2776-2778	,	denotes	,
T9496	2778-2782	VBP	denotes	bear
T9531	2783-2787	JJ	denotes	much
T9532	2788-2798	NN	denotes	similarity
T9533	2799-2801	IN	denotes	to
T9534	2802-2805	DT	denotes	the
T9536	2806-2814	RB	denotes	recently
T9537	2815-2821	VBN	denotes	solved
T9535	2822-2831	NN	denotes	structure
T9538	2832-2834	IN	denotes	of
T9539	2835-2838	DT	denotes	the
T9541	2839-2843	NN	denotes	SV40
T9542	2844-2849	JJ	denotes	large
T9543	2850-2851	NN	denotes	T
T9544	2851-2852	HYPH	denotes	-
T9540	2852-2859	NN	denotes	antigen
T9545	2860-2861	-LRB-	denotes	(
T9547	2861-2862	CD	denotes	4
T9548	2862-2863	,	denotes	,
T9546	2863-2864	CD	denotes	6
T9549	2864-2865	-RRB-	denotes	)
T9550	2865-2867	,	denotes	,
T9551	2867-2870	CC	denotes	and
T9552	2871-2874	VBP	denotes	are
T9553	2875-2877	IN	denotes	in
T9554	2878-2883	NN	denotes	favor
T9555	2884-2886	IN	denotes	of
T9556	2887-2890	DT	denotes	the
T9558	2891-2897	JJ	denotes	double
T9560	2897-2898	HYPH	denotes	-
T9559	2898-2907	JJ	denotes	hexameric
T9557	2908-2917	NN	denotes	structure
T9561	2918-2920	IN	denotes	of
T9562	2921-2926	NN	denotes	mouse
T9563	2927-2930	NN	denotes	Mcm
T9564	2931-2933	IN	denotes	on
T9565	2934-2935	DT	denotes	a
T9567	2936-2942	NN	denotes	bubble
T9566	2943-2946	NN	denotes	DNA
T9568	2946-2947	.	denotes	.
T9856	2949-2958	NN	denotes	Substrate
T9858	2959-2962	CC	denotes	and
T9859	2963-2971	NN	denotes	sequence
T9857	2972-2983	NN	denotes	requirement
T9860	2984-2987	IN	denotes	for
T9861	2988-2992	NN	denotes	Mcm4
T9863	2992-2993	HYPH	denotes	/
T9864	2993-2994	CD	denotes	6
T9865	2994-2995	HYPH	denotes	/
T9866	2995-2996	CD	denotes	7
T9867	2997-3005	NN	denotes	helicase
T9862	3006-3016	NN	denotes	activation
T9868	3016-3194	sentence	denotes	DNA binding assays indicate that Mcm4/6/7 binds to those substrates containing single-stranded DNA regions regardless of the presence or absence of single-stranded 3′ or 5′ end.
T9869	3017-3020	NN	denotes	DNA
T9870	3021-3028	NN	denotes	binding
T9871	3029-3035	NNS	denotes	assays
T9872	3036-3044	VBP	denotes	indicate
T9873	3045-3049	IN	denotes	that
T9875	3050-3054	NN	denotes	Mcm4
T9876	3054-3055	HYPH	denotes	/
T9877	3055-3056	CD	denotes	6
T9878	3056-3057	HYPH	denotes	/
T9879	3057-3058	CD	denotes	7
T9874	3059-3064	VBZ	denotes	binds
T9880	3065-3067	IN	denotes	to
T9881	3068-3073	DT	denotes	those
T9882	3074-3084	NNS	denotes	substrates
T9883	3085-3095	VBG	denotes	containing
T9884	3096-3102	JJ	denotes	single
T9886	3102-3103	HYPH	denotes	-
T9885	3103-3111	VBN	denotes	stranded
T9888	3112-3115	NN	denotes	DNA
T9887	3116-3123	NNS	denotes	regions
T9889	3124-3134	RB	denotes	regardless
T9890	3135-3137	IN	denotes	of
T9891	3138-3141	DT	denotes	the
T9892	3142-3150	NN	denotes	presence
T9893	3151-3153	CC	denotes	or
T9894	3154-3161	NN	denotes	absence
T9895	3162-3164	IN	denotes	of
T9896	3165-3171	JJ	denotes	single
T9898	3171-3172	HYPH	denotes	-
T9897	3172-3180	VBN	denotes	stranded
T9900	3181-3182	CD	denotes	3
T9901	3182-3183	SYM	denotes	′
T9902	3184-3186	CC	denotes	or
T9903	3187-3188	CD	denotes	5
T9904	3188-3189	SYM	denotes	′
T9899	3190-3193	NN	denotes	end
T9905	3193-3194	.	denotes	.
T9906	3194-3295	sentence	denotes	Unwinding of the duplex DNA depends on translocation of single-stranded DNA from 3′ to 5′ direction.
T9907	3195-3204	NN	denotes	Unwinding
T9909	3205-3207	IN	denotes	of
T9910	3208-3211	DT	denotes	the
T9912	3212-3218	NN	denotes	duplex
T9911	3219-3222	NN	denotes	DNA
T9908	3223-3230	VBZ	denotes	depends
T9913	3231-3233	IN	denotes	on
T9914	3234-3247	NN	denotes	translocation
T9915	3248-3250	IN	denotes	of
T9916	3251-3257	JJ	denotes	single
T9918	3257-3258	HYPH	denotes	-
T9917	3258-3266	VBN	denotes	stranded
T9919	3267-3270	NN	denotes	DNA
T9920	3271-3275	IN	denotes	from
T9921	3276-3277	CD	denotes	3
T9922	3277-3278	SYM	denotes	′
T9923	3279-3281	IN	denotes	to
T9924	3282-3283	CD	denotes	5
T9925	3283-3284	SYM	denotes	′
T9926	3285-3294	NN	denotes	direction
T9927	3294-3295	.	denotes	.
T9928	3295-3431	sentence	denotes	This is most clearly shown by its helicase action on A-fork[5′] and 3′-extension but not on A-fork[3′], A-fork[3′,5′] nor 5′-extension.
T9929	3296-3300	DT	denotes	This
T9931	3301-3303	VBZ	denotes	is
T9932	3304-3308	RBS	denotes	most
T9933	3309-3316	RB	denotes	clearly
T9930	3317-3322	VBN	denotes	shown
T9934	3323-3325	IN	denotes	by
T9935	3326-3329	PRP$	denotes	its
T9937	3330-3338	NN	denotes	helicase
T9936	3339-3345	NN	denotes	action
T9938	3346-3348	IN	denotes	on
T9939	3349-3350	NN	denotes	A
T9941	3350-3351	HYPH	denotes	-
T9940	3351-3355	NN	denotes	fork
T9943	3355-3356	-LRB-	denotes	[
T9944	3356-3357	CD	denotes	5
T9945	3357-3358	SYM	denotes	′
T9946	3358-3359	-RRB-	denotes	]
T9947	3360-3363	CC	denotes	and
T9948	3364-3365	CD	denotes	3
T9949	3365-3366	SYM	denotes	′
T9950	3366-3367	HYPH	denotes	-
T9942	3367-3376	NN	denotes	extension
T9951	3377-3380	CC	denotes	but
T9952	3381-3384	RB	denotes	not
T9953	3385-3387	IN	denotes	on
T9954	3388-3389	NN	denotes	A
T9956	3389-3390	HYPH	denotes	-
T9955	3390-3394	NN	denotes	fork
T9958	3394-3395	-LRB-	denotes	[
T9959	3395-3396	CD	denotes	3
T9960	3396-3397	SYM	denotes	′
T9961	3397-3398	-RRB-	denotes	]
T9962	3398-3400	,	denotes	,
T9963	3400-3401	NN	denotes	A
T9965	3401-3402	HYPH	denotes	-
T9964	3402-3406	NN	denotes	fork
T9966	3406-3407	-LRB-	denotes	[
T9967	3407-3408	CD	denotes	3
T9968	3408-3409	SYM	denotes	′
T9969	3409-3410	,	denotes	,
T9970	3410-3411	CD	denotes	5
T9971	3411-3412	SYM	denotes	′
T9972	3412-3413	-RRB-	denotes	]
T9973	3414-3417	CC	denotes	nor
T9974	3418-3419	CD	denotes	5
T9975	3419-3420	SYM	denotes	′
T9976	3420-3421	HYPH	denotes	-
T9957	3421-3430	NN	denotes	extension
T9977	3430-3431	.	denotes	.
T9978	3431-3592	sentence	denotes	However, it does not necessarily require 3′ end of single-stranded DNA, since Mcm4/6/7 can displace annealing oligonucleotide on a circular single-stranded DNA.
T9979	3432-3439	RB	denotes	However
T9981	3439-3441	,	denotes	,
T9982	3441-3443	PRP	denotes	it
T9983	3444-3448	VBZ	denotes	does
T9984	3449-3452	RB	denotes	not
T9985	3453-3464	RB	denotes	necessarily
T9980	3465-3472	VB	denotes	require
T9986	3473-3474	CD	denotes	3
T9988	3474-3475	SYM	denotes	′
T9987	3476-3479	NN	denotes	end
T9989	3480-3482	IN	denotes	of
T9990	3483-3489	JJ	denotes	single
T9992	3489-3490	HYPH	denotes	-
T9991	3490-3498	VBN	denotes	stranded
T9993	3499-3502	NN	denotes	DNA
T9994	3502-3504	,	denotes	,
T9995	3504-3509	IN	denotes	since
T9997	3510-3514	NN	denotes	Mcm4
T9998	3514-3515	HYPH	denotes	/
T9999	3515-3516	CD	denotes	6
T10000	3516-3517	HYPH	denotes	/
T10001	3517-3518	CD	denotes	7
T10002	3519-3522	MD	denotes	can
T9996	3523-3531	VB	denotes	displace
T10003	3532-3541	VBG	denotes	annealing
T10004	3542-3557	NN	denotes	oligonucleotide
T10005	3558-3560	IN	denotes	on
T10006	3561-3562	DT	denotes	a
T10008	3563-3571	JJ	denotes	circular
T10009	3572-3578	JJ	denotes	single
T10011	3578-3579	HYPH	denotes	-
T10010	3579-3587	VBN	denotes	stranded
T10007	3588-3591	NN	denotes	DNA
T10012	3591-3592	.	denotes	.
T10013	3592-3750	sentence	denotes	The ability of the mouse Mcm4/6/7 to unwind 3′-extension is shared by the archaeal Mcm helicase but not by Mcm4/6/7 from S.pombe and S.cerevisiae (14,26,27).
T10014	3593-3596	DT	denotes	The
T10015	3597-3604	NN	denotes	ability
T10017	3605-3607	IN	denotes	of
T10018	3608-3611	DT	denotes	the
T10020	3612-3617	NN	denotes	mouse
T10019	3618-3622	NN	denotes	Mcm4
T10021	3622-3623	HYPH	denotes	/
T10022	3623-3624	CD	denotes	6
T10023	3624-3625	HYPH	denotes	/
T10024	3625-3626	CD	denotes	7
T10025	3627-3629	TO	denotes	to
T10026	3630-3636	VB	denotes	unwind
T10027	3637-3638	CD	denotes	3
T10029	3638-3639	SYM	denotes	′
T10030	3639-3640	HYPH	denotes	-
T10028	3640-3649	NN	denotes	extension
T10031	3650-3652	VBZ	denotes	is
T10016	3653-3659	VBN	denotes	shared
T10032	3660-3662	IN	denotes	by
T10033	3663-3666	DT	denotes	the
T10035	3667-3675	JJ	denotes	archaeal
T10036	3676-3679	NN	denotes	Mcm
T10034	3680-3688	NN	denotes	helicase
T10037	3689-3692	CC	denotes	but
T10038	3693-3696	RB	denotes	not
T10039	3697-3699	IN	denotes	by
T10040	3700-3704	NN	denotes	Mcm4
T10041	3704-3705	HYPH	denotes	/
T10042	3705-3706	CD	denotes	6
T10043	3706-3707	HYPH	denotes	/
T10044	3707-3708	CD	denotes	7
T10045	3709-3713	IN	denotes	from
T10046	3714-3721	NNP	denotes	S.pombe
T10047	3722-3725	CC	denotes	and
T10048	3726-3738	NNP	denotes	S.cerevisiae
T10049	3739-3740	-LRB-	denotes	(
T10051	3740-3742	CD	denotes	14
T10052	3742-3743	,	denotes	,
T10053	3743-3745	CD	denotes	26
T10054	3745-3746	,	denotes	,
T10050	3746-3748	CD	denotes	27
T10055	3748-3749	-RRB-	denotes	)
T10056	3749-3750	.	denotes	.
T10057	3750-3899	sentence	denotes	The archaeal Mcm can unwind A-fork[3′] but eukaryotic Mcm4/6/7 cannot, since the former binds to double-stranded DNA but the latter does not (5,27).
T10058	3751-3754	DT	denotes	The
T10060	3755-3763	JJ	denotes	archaeal
T10059	3764-3767	NN	denotes	Mcm
T10062	3768-3771	MD	denotes	can
T10061	3772-3778	VB	denotes	unwind
T10063	3779-3780	NN	denotes	A
T10065	3780-3781	HYPH	denotes	-
T10064	3781-3785	NN	denotes	fork
T10066	3785-3786	-LRB-	denotes	[
T10067	3786-3787	CD	denotes	3
T10068	3787-3788	SYM	denotes	′
T10069	3788-3789	-RRB-	denotes	]
T10070	3790-3793	CC	denotes	but
T10071	3794-3804	JJ	denotes	eukaryotic
T10072	3805-3809	NN	denotes	Mcm4
T10074	3809-3810	HYPH	denotes	/
T10075	3810-3811	CD	denotes	6
T10076	3811-3812	HYPH	denotes	/
T10077	3812-3813	CD	denotes	7
T10073	3814-3817	MD	denotes	can
T10078	3817-3820	RB	denotes	not
T10079	3820-3822	,	denotes	,
T10080	3822-3827	IN	denotes	since
T10082	3828-3831	DT	denotes	the
T10083	3832-3838	NN	denotes	former
T10081	3839-3844	VBZ	denotes	binds
T10084	3845-3847	IN	denotes	to
T10085	3848-3854	JJ	denotes	double
T10087	3854-3855	HYPH	denotes	-
T10086	3855-3863	VBN	denotes	stranded
T10088	3864-3867	NN	denotes	DNA
T10089	3868-3871	CC	denotes	but
T10090	3872-3875	DT	denotes	the
T10091	3876-3882	JJ	denotes	latter
T10093	3883-3887	VBZ	denotes	does
T10094	3888-3891	RB	denotes	not
T10095	3892-3893	-LRB-	denotes	(
T10096	3893-3894	CD	denotes	5
T10097	3894-3895	,	denotes	,
T10092	3895-3897	CD	denotes	27
T10098	3897-3898	-RRB-	denotes	)
T10099	3898-3899	.	denotes	.
T10100	3899-4029	sentence	denotes	While yeast Mcm helicases can translocate on duplex DNA, such activity was not observed with mammalian Mcm4/6/7 (data not shown).
T10101	3900-3905	IN	denotes	While
T10103	3906-3911	NN	denotes	yeast
T10104	3912-3915	NN	denotes	Mcm
T10105	3916-3925	NNS	denotes	helicases
T10106	3926-3929	MD	denotes	can
T10102	3930-3941	VB	denotes	translocate
T10108	3942-3944	IN	denotes	on
T10109	3945-3951	NN	denotes	duplex
T10110	3952-3955	NN	denotes	DNA
T10111	3955-3957	,	denotes	,
T10112	3957-3961	JJ	denotes	such
T10113	3962-3970	NN	denotes	activity
T10114	3971-3974	VBD	denotes	was
T10115	3975-3978	RB	denotes	not
T10107	3979-3987	VBN	denotes	observed
T10116	3988-3992	IN	denotes	with
T10117	3993-4002	JJ	denotes	mammalian
T10118	4003-4007	NN	denotes	Mcm4
T10119	4007-4008	HYPH	denotes	/
T10120	4008-4009	CD	denotes	6
T10121	4009-4010	HYPH	denotes	/
T10122	4010-4011	CD	denotes	7
T10123	4012-4013	-LRB-	denotes	(
T10125	4013-4017	NNS	denotes	data
T10126	4018-4021	RB	denotes	not
T10124	4022-4027	VBN	denotes	shown
T10127	4027-4028	-RRB-	denotes	)
T10128	4028-4029	.	denotes	.
T10129	4029-4366	sentence	denotes	Occurrence of AT-rich sequences, with asymmetric distribution of adenine and thymine, near the replication origins, lead us to propose that Mcm may play a role in selection of initiation sites of mammalian DNA replication, and prompted us to examine the ability of sequences from human replication origins to activate Mcm4/6/7 helicase.
T10130	4030-4040	NN	denotes	Occurrence
T10132	4041-4043	IN	denotes	of
T10133	4044-4046	NN	denotes	AT
T10135	4046-4047	HYPH	denotes	-
T10134	4047-4051	JJ	denotes	rich
T10136	4052-4061	NNS	denotes	sequences
T10137	4061-4063	,	denotes	,
T10138	4063-4067	IN	denotes	with
T10139	4068-4078	JJ	denotes	asymmetric
T10140	4079-4091	NN	denotes	distribution
T10141	4092-4094	IN	denotes	of
T10142	4095-4102	NN	denotes	adenine
T10143	4103-4106	CC	denotes	and
T10144	4107-4114	NN	denotes	thymine
T10145	4114-4116	,	denotes	,
T10146	4116-4120	IN	denotes	near
T10147	4121-4124	DT	denotes	the
T10149	4125-4136	NN	denotes	replication
T10148	4137-4144	NNS	denotes	origins
T10150	4144-4146	,	denotes	,
T10131	4146-4150	VBP	denotes	lead
T10151	4151-4153	PRP	denotes	us
T10152	4154-4156	TO	denotes	to
T10153	4157-4164	VB	denotes	propose
T10154	4165-4169	IN	denotes	that
T10156	4170-4173	NNP	denotes	Mcm
T10157	4174-4177	MD	denotes	may
T10155	4178-4182	VB	denotes	play
T10158	4183-4184	DT	denotes	a
T10159	4185-4189	NN	denotes	role
T10160	4190-4192	IN	denotes	in
T10161	4193-4202	NN	denotes	selection
T10162	4203-4205	IN	denotes	of
T10163	4206-4216	NN	denotes	initiation
T10164	4217-4222	NNS	denotes	sites
T10165	4223-4225	IN	denotes	of
T10166	4226-4235	JJ	denotes	mammalian
T10167	4236-4239	NN	denotes	DNA
T10168	4240-4251	NN	denotes	replication
T10169	4251-4253	,	denotes	,
T10170	4253-4256	CC	denotes	and
T10171	4257-4265	VBD	denotes	prompted
T10172	4266-4268	PRP	denotes	us
T10173	4269-4271	TO	denotes	to
T10174	4272-4279	VB	denotes	examine
T10175	4280-4283	DT	denotes	the
T10176	4284-4291	NN	denotes	ability
T10177	4292-4294	IN	denotes	of
T10178	4295-4304	NNS	denotes	sequences
T10179	4305-4309	IN	denotes	from
T10180	4310-4315	JJ	denotes	human
T10182	4316-4327	NN	denotes	replication
T10181	4328-4335	NNS	denotes	origins
T10183	4336-4338	TO	denotes	to
T10184	4339-4347	VB	denotes	activate
T10185	4348-4352	NN	denotes	Mcm4
T10187	4352-4353	HYPH	denotes	/
T10188	4353-4354	CD	denotes	6
T10189	4354-4355	HYPH	denotes	/
T10190	4355-4356	CD	denotes	7
T10186	4357-4365	NN	denotes	helicase
T10191	4365-4366	.	denotes	.
T10192	4366-4455	sentence	denotes	Both lamin-B2 and c-myc origins served as efficient activator for Mcm helicase in vitro.
T10193	4367-4371	CC	denotes	Both
T10195	4372-4377	NN	denotes	lamin
T10196	4377-4378	HYPH	denotes	-
T10194	4378-4380	NN	denotes	B2
T10198	4381-4384	CC	denotes	and
T10199	4385-4386	NN	denotes	c
T10201	4386-4387	HYPH	denotes	-
T10200	4387-4390	NN	denotes	myc
T10197	4391-4398	NNS	denotes	origins
T10202	4399-4405	VBD	denotes	served
T10203	4406-4408	IN	denotes	as
T10204	4409-4418	JJ	denotes	efficient
T10205	4419-4428	NN	denotes	activator
T10206	4429-4432	IN	denotes	for
T10207	4433-4436	NN	denotes	Mcm
T10208	4437-4445	NN	denotes	helicase
T10209	4446-4448	FW	denotes	in
T10210	4449-4454	FW	denotes	vitro
T10211	4454-4455	.	denotes	.
T10212	4455-4584	sentence	denotes	Consistent with it, site-specific loading of Mcm in the DNA replication initiation zone of the c-myc was recently reported (33).
T10213	4456-4466	JJ	denotes	Consistent
T10215	4467-4471	IN	denotes	with
T10216	4472-4474	PRP	denotes	it
T10217	4474-4476	,	denotes	,
T10218	4476-4480	NN	denotes	site
T10220	4480-4481	HYPH	denotes	-
T10219	4481-4489	JJ	denotes	specific
T10221	4490-4497	NN	denotes	loading
T10222	4498-4500	IN	denotes	of
T10223	4501-4504	NN	denotes	Mcm
T10224	4505-4507	IN	denotes	in
T10225	4508-4511	DT	denotes	the
T10227	4512-4515	NN	denotes	DNA
T10229	4516-4527	NN	denotes	replication
T10228	4528-4538	NN	denotes	initiation
T10226	4539-4543	NN	denotes	zone
T10230	4544-4546	IN	denotes	of
T10231	4547-4550	DT	denotes	the
T10233	4551-4552	NN	denotes	c
T10234	4552-4553	HYPH	denotes	-
T10232	4553-4556	NN	denotes	myc
T10235	4557-4560	VBD	denotes	was
T10236	4561-4569	RB	denotes	recently
T10214	4570-4578	VBN	denotes	reported
T10237	4579-4580	-LRB-	denotes	(
T10238	4580-4582	CD	denotes	33
T10239	4582-4583	-RRB-	denotes	)
T10240	4583-4584	.	denotes	.
T10241	4584-4702	sentence	denotes	We have examined in detail the effect of sequence context of the single-stranded DNA on the helicase activity of Mcm.
T10242	4585-4587	PRP	denotes	We
T10244	4588-4592	VBP	denotes	have
T10243	4593-4601	VBN	denotes	examined
T10245	4602-4604	IN	denotes	in
T10246	4605-4611	NN	denotes	detail
T10247	4612-4615	DT	denotes	the
T10248	4616-4622	NN	denotes	effect
T10249	4623-4625	IN	denotes	of
T10250	4626-4634	NN	denotes	sequence
T10251	4635-4642	NN	denotes	context
T10252	4643-4645	IN	denotes	of
T10253	4646-4649	DT	denotes	the
T10255	4650-4656	JJ	denotes	single
T10257	4656-4657	HYPH	denotes	-
T10256	4657-4665	VBN	denotes	stranded
T10254	4666-4669	NN	denotes	DNA
T10258	4670-4672	IN	denotes	on
T10259	4673-4676	DT	denotes	the
T10261	4677-4685	NN	denotes	helicase
T10260	4686-4694	NN	denotes	activity
T10262	4695-4697	IN	denotes	of
T10263	4698-4701	NN	denotes	Mcm
T10264	4701-4702	.	denotes	.
T10265	4702-4800	sentence	denotes	The results indicate that thymine content of 50% is sufficient for the maximum helicase activity.
T10266	4703-4706	DT	denotes	The
T10267	4707-4714	NNS	denotes	results
T10268	4715-4723	VBP	denotes	indicate
T10269	4724-4728	IN	denotes	that
T10271	4729-4736	NN	denotes	thymine
T10272	4737-4744	NN	denotes	content
T10273	4745-4747	IN	denotes	of
T10274	4748-4750	CD	denotes	50
T10275	4750-4751	NN	denotes	%
T10270	4752-4754	VBZ	denotes	is
T10276	4755-4765	JJ	denotes	sufficient
T10277	4766-4769	IN	denotes	for
T10278	4770-4773	DT	denotes	the
T10280	4774-4781	JJ	denotes	maximum
T10281	4782-4790	NN	denotes	helicase
T10279	4791-4799	NN	denotes	activity
T10282	4799-4800	.	denotes	.
T10283	4800-4906	sentence	denotes	The efficiency of displacement decreased as the thymine content of the 3′-tail dropped to 33% (Figure 6).
T10284	4801-4804	DT	denotes	The
T10285	4805-4815	NN	denotes	efficiency
T10287	4816-4818	IN	denotes	of
T10288	4819-4831	NN	denotes	displacement
T10286	4832-4841	VBD	denotes	decreased
T10289	4842-4844	IN	denotes	as
T10291	4845-4848	DT	denotes	the
T10293	4849-4856	NN	denotes	thymine
T10292	4857-4864	NN	denotes	content
T10294	4865-4867	IN	denotes	of
T10295	4868-4871	DT	denotes	the
T10297	4872-4873	CD	denotes	3
T10298	4873-4874	SYM	denotes	′
T10299	4874-4875	HYPH	denotes	-
T10296	4875-4879	NN	denotes	tail
T10290	4880-4887	VBD	denotes	dropped
T10300	4888-4890	IN	denotes	to
T10301	4891-4893	CD	denotes	33
T10302	4893-4894	NN	denotes	%
T10303	4895-4896	-LRB-	denotes	(
T10304	4896-4902	NN	denotes	Figure
T10305	4903-4904	CD	denotes	6
T10306	4904-4905	-RRB-	denotes	)
T10307	4905-4906	.	denotes	.
T10308	4906-5050	sentence	denotes	The stretches of thymine residues may not be necessarily required, since repeats of TC dinucleotides served as a potent activator for Mcm4/6/7.
T10309	4907-4910	DT	denotes	The
T10310	4911-4920	NNS	denotes	stretches
T10312	4921-4923	IN	denotes	of
T10313	4924-4931	NN	denotes	thymine
T10314	4932-4940	NNS	denotes	residues
T10315	4941-4944	MD	denotes	may
T10316	4945-4948	RB	denotes	not
T10317	4949-4951	VB	denotes	be
T10318	4952-4963	RB	denotes	necessarily
T10311	4964-4972	VBN	denotes	required
T10319	4972-4974	,	denotes	,
T10320	4974-4979	IN	denotes	since
T10322	4980-4987	NNS	denotes	repeats
T10323	4988-4990	IN	denotes	of
T10324	4991-4993	NN	denotes	TC
T10325	4994-5007	NNS	denotes	dinucleotides
T10321	5008-5014	VBD	denotes	served
T10326	5015-5017	IN	denotes	as
T10327	5018-5019	DT	denotes	a
T10329	5020-5026	JJ	denotes	potent
T10328	5027-5036	NN	denotes	activator
T10330	5037-5040	IN	denotes	for
T10331	5041-5045	NN	denotes	Mcm4
T10332	5045-5046	HYPH	denotes	/
T10333	5046-5047	CD	denotes	6
T10334	5047-5048	HYPH	denotes	/
T10335	5048-5049	CD	denotes	7
T10336	5049-5050	.	denotes	.
T10337	5050-5175	sentence	denotes	We also noticed that the presence of a secondary structure within the single-stranded DNA is inhibitory for helicase action.
T10338	5051-5053	PRP	denotes	We
T10340	5054-5058	RB	denotes	also
T10339	5059-5066	VBD	denotes	noticed
T10341	5067-5071	IN	denotes	that
T10343	5072-5075	DT	denotes	the
T10344	5076-5084	NN	denotes	presence
T10345	5085-5087	IN	denotes	of
T10346	5088-5089	DT	denotes	a
T10348	5090-5099	JJ	denotes	secondary
T10347	5100-5109	NN	denotes	structure
T10349	5110-5116	IN	denotes	within
T10350	5117-5120	DT	denotes	the
T10352	5121-5127	JJ	denotes	single
T10354	5127-5128	HYPH	denotes	-
T10353	5128-5136	VBN	denotes	stranded
T10351	5137-5140	NN	denotes	DNA
T10342	5141-5143	VBZ	denotes	is
T10355	5144-5154	JJ	denotes	inhibitory
T10356	5155-5158	IN	denotes	for
T10357	5159-5167	NN	denotes	helicase
T10358	5168-5174	NN	denotes	action
T10359	5174-5175	.	denotes	.
T10360	5175-5287	sentence	denotes	Nuclease footprinting assays indicated that binding was interfered by the secondary structure (data not shown).
T10361	5176-5184	NN	denotes	Nuclease
T10362	5185-5197	NN	denotes	footprinting
T10363	5198-5204	NNS	denotes	assays
T10364	5205-5214	VBD	denotes	indicated
T10365	5215-5219	IN	denotes	that
T10367	5220-5227	NN	denotes	binding
T10368	5228-5231	VBD	denotes	was
T10366	5232-5242	VBN	denotes	interfered
T10369	5243-5245	IN	denotes	by
T10370	5246-5249	DT	denotes	the
T10372	5250-5259	JJ	denotes	secondary
T10371	5260-5269	NN	denotes	structure
T10373	5270-5271	-LRB-	denotes	(
T10375	5271-5275	NNS	denotes	data
T10376	5276-5279	RB	denotes	not
T10374	5280-5285	VBN	denotes	shown
T10377	5285-5286	-RRB-	denotes	)
T10378	5286-5287	.	denotes	.
T10379	5287-5695	sentence	denotes	Thus, we have concluded that Mcm4/6/7 helicase is most efficiently activated by non-structured single-stranded DNA with thymine content of 50% or more, although significant stimulation is observed also by DNA with less thymine content (Figure 7), suggesting that the sequence specificity for Mcm helicase activation is rather relaxed and that the extent of the activation may depend on the sequence context.
T10380	5288-5292	RB	denotes	Thus
T10382	5292-5294	,	denotes	,
T10383	5294-5296	PRP	denotes	we
T10384	5297-5301	VBP	denotes	have
T10381	5302-5311	VBN	denotes	concluded
T10385	5312-5316	IN	denotes	that
T10387	5317-5321	NN	denotes	Mcm4
T10389	5321-5322	HYPH	denotes	/
T10390	5322-5323	CD	denotes	6
T10391	5323-5324	HYPH	denotes	/
T10392	5324-5325	CD	denotes	7
T10388	5326-5334	NN	denotes	helicase
T10393	5335-5337	VBZ	denotes	is
T10394	5338-5342	RBS	denotes	most
T10395	5343-5354	RB	denotes	efficiently
T10386	5355-5364	VBN	denotes	activated
T10396	5365-5367	IN	denotes	by
T10397	5368-5382	JJ	denotes	non-structured
T10399	5383-5389	JJ	denotes	single
T10401	5389-5390	HYPH	denotes	-
T10400	5390-5398	VBN	denotes	stranded
T10398	5399-5402	NN	denotes	DNA
T10402	5403-5407	IN	denotes	with
T10403	5408-5415	NN	denotes	thymine
T10404	5416-5423	NN	denotes	content
T10405	5424-5426	IN	denotes	of
T10406	5427-5429	CD	denotes	50
T10407	5429-5430	NN	denotes	%
T10408	5431-5433	CC	denotes	or
T10409	5434-5438	JJR	denotes	more
T10410	5438-5440	,	denotes	,
T10411	5440-5448	IN	denotes	although
T10413	5449-5460	JJ	denotes	significant
T10414	5461-5472	NN	denotes	stimulation
T10415	5473-5475	VBZ	denotes	is
T10412	5476-5484	VBN	denotes	observed
T10416	5485-5489	RB	denotes	also
T10417	5490-5492	IN	denotes	by
T10418	5493-5496	NN	denotes	DNA
T10419	5497-5501	IN	denotes	with
T10420	5502-5506	JJR	denotes	less
T10422	5507-5514	NN	denotes	thymine
T10421	5515-5522	NN	denotes	content
T10423	5523-5524	-LRB-	denotes	(
T10424	5524-5530	NN	denotes	Figure
T10425	5531-5532	CD	denotes	7
T10426	5532-5533	-RRB-	denotes	)
T10427	5533-5535	,	denotes	,
T10428	5535-5545	VBG	denotes	suggesting
T10429	5546-5550	IN	denotes	that
T10431	5551-5554	DT	denotes	the
T10433	5555-5563	NN	denotes	sequence
T10432	5564-5575	NN	denotes	specificity
T10434	5576-5579	IN	denotes	for
T10435	5580-5583	NN	denotes	Mcm
T10436	5584-5592	NN	denotes	helicase
T10437	5593-5603	NN	denotes	activation
T10430	5604-5606	VBZ	denotes	is
T10438	5607-5613	RB	denotes	rather
T10439	5614-5621	JJ	denotes	relaxed
T10440	5622-5625	CC	denotes	and
T10441	5626-5630	IN	denotes	that
T10443	5631-5634	DT	denotes	the
T10444	5635-5641	NN	denotes	extent
T10445	5642-5644	IN	denotes	of
T10446	5645-5648	DT	denotes	the
T10447	5649-5659	NN	denotes	activation
T10448	5660-5663	MD	denotes	may
T10442	5664-5670	VB	denotes	depend
T10449	5671-5673	IN	denotes	on
T10450	5674-5677	DT	denotes	the
T10452	5678-5686	NN	denotes	sequence
T10451	5687-5694	NN	denotes	context
T10453	5694-5695	.	denotes	.
T10454	5695-5993	sentence	denotes	This would be reasonable given the flexibility and differential regulation of site selection for initiation of DNA replication during development or in various cell types, as well as the variability in initiation potential of each replication origin on the genome even within the single cell type.
T10455	5696-5700	DT	denotes	This
T10457	5701-5706	MD	denotes	would
T10456	5707-5709	VB	denotes	be
T10458	5710-5720	JJ	denotes	reasonable
T10459	5721-5726	VBN	denotes	given
T10460	5727-5730	DT	denotes	the
T10461	5731-5742	NN	denotes	flexibility
T10462	5743-5746	CC	denotes	and
T10463	5747-5759	JJ	denotes	differential
T10464	5760-5770	NN	denotes	regulation
T10465	5771-5773	IN	denotes	of
T10466	5774-5778	NN	denotes	site
T10467	5779-5788	NN	denotes	selection
T10468	5789-5792	IN	denotes	for
T10469	5793-5803	NN	denotes	initiation
T10470	5804-5806	IN	denotes	of
T10471	5807-5810	NN	denotes	DNA
T10472	5811-5822	NN	denotes	replication
T10473	5823-5829	IN	denotes	during
T10474	5830-5841	NN	denotes	development
T10475	5842-5844	CC	denotes	or
T10476	5845-5847	IN	denotes	in
T10477	5848-5855	JJ	denotes	various
T10479	5856-5860	NN	denotes	cell
T10478	5861-5866	NNS	denotes	types
T10480	5866-5868	,	denotes	,
T10481	5868-5870	RB	denotes	as
T10483	5871-5875	RB	denotes	well
T10482	5876-5878	IN	denotes	as
T10484	5879-5882	DT	denotes	the
T10485	5883-5894	NN	denotes	variability
T10486	5895-5897	IN	denotes	in
T10487	5898-5908	NN	denotes	initiation
T10488	5909-5918	NN	denotes	potential
T10489	5919-5921	IN	denotes	of
T10490	5922-5926	DT	denotes	each
T10492	5927-5938	NN	denotes	replication
T10491	5939-5945	NN	denotes	origin
T10493	5946-5948	IN	denotes	on
T10494	5949-5952	DT	denotes	the
T10495	5953-5959	NN	denotes	genome
T10496	5960-5964	RB	denotes	even
T10497	5965-5971	IN	denotes	within
T10498	5972-5975	DT	denotes	the
T10500	5976-5982	JJ	denotes	single
T10501	5983-5987	NN	denotes	cell
T10499	5988-5992	NN	denotes	type
T10502	5992-5993	.	denotes	.
T10855	5995-5999	NN	denotes	Mcm4
T10857	5999-6000	HYPH	denotes	/
T10858	6000-6001	CD	denotes	6
T10859	6001-6002	HYPH	denotes	/
T10860	6002-6003	CD	denotes	7
T10856	6004-6012	NN	denotes	helicase
T10861	6013-6019	IN	denotes	during
T10862	6020-6030	JJ	denotes	processive
T10863	6031-6040	NN	denotes	unwinding
T10864	6041-6043	IN	denotes	at
T10865	6044-6047	DT	denotes	the
T10866	6048-6052	NN	denotes	fork
T10867	6052-6238	sentence	denotes	The specific requirement of single-stranded thymine residues for activation of Mcm helicase prompted us to examine whether they are required also for processive unwinding of duplex DNA.
T10868	6053-6056	DT	denotes	The
T10870	6057-6065	JJ	denotes	specific
T10869	6066-6077	NN	denotes	requirement
T10872	6078-6080	IN	denotes	of
T10873	6081-6087	JJ	denotes	single
T10875	6087-6088	HYPH	denotes	-
T10874	6088-6096	VBN	denotes	stranded
T10877	6097-6104	NN	denotes	thymine
T10876	6105-6113	NNS	denotes	residues
T10878	6114-6117	IN	denotes	for
T10879	6118-6128	NN	denotes	activation
T10880	6129-6131	IN	denotes	of
T10881	6132-6135	NN	denotes	Mcm
T10882	6136-6144	NN	denotes	helicase
T10871	6145-6153	VBD	denotes	prompted
T10883	6154-6156	PRP	denotes	us
T10884	6157-6159	TO	denotes	to
T10885	6160-6167	VB	denotes	examine
T10886	6168-6175	IN	denotes	whether
T10888	6176-6180	PRP	denotes	they
T10889	6181-6184	VBP	denotes	are
T10887	6185-6193	VBN	denotes	required
T10890	6194-6198	RB	denotes	also
T10891	6199-6202	IN	denotes	for
T10892	6203-6213	JJ	denotes	processive
T10893	6214-6223	NN	denotes	unwinding
T10894	6224-6226	IN	denotes	of
T10895	6227-6233	NN	denotes	duplex
T10896	6234-6237	NN	denotes	DNA
T10897	6237-6238	.	denotes	.
T10898	6238-6358	sentence	denotes	Our results indicated that increase of GC pair in the duplex segment significantly inhibited the Mcm helicase activity.
T10899	6239-6242	PRP$	denotes	Our
T10900	6243-6250	NNS	denotes	results
T10901	6251-6260	VBD	denotes	indicated
T10902	6261-6265	IN	denotes	that
T10904	6266-6274	NN	denotes	increase
T10905	6275-6277	IN	denotes	of
T10906	6278-6280	NN	denotes	GC
T10907	6281-6285	NN	denotes	pair
T10908	6286-6288	IN	denotes	in
T10909	6289-6292	DT	denotes	the
T10911	6293-6299	NN	denotes	duplex
T10910	6300-6307	NN	denotes	segment
T10912	6308-6321	RB	denotes	significantly
T10903	6322-6331	VBD	denotes	inhibited
T10913	6332-6335	DT	denotes	the
T10915	6336-6339	NN	denotes	Mcm
T10916	6340-6348	NN	denotes	helicase
T10914	6349-6357	NN	denotes	activity
T10917	6357-6358	.	denotes	.
T10918	6358-6530	sentence	denotes	Duplex DNA composed only of GC pairs (10 repeats of CGG) on a Y-fork was not displaced at all, while the same template was readily displaced by SV40 T-antigen (Figure 8B).
T10919	6359-6365	NN	denotes	Duplex
T10920	6366-6369	NN	denotes	DNA
T10922	6370-6378	VBN	denotes	composed
T10923	6379-6383	RB	denotes	only
T10924	6384-6386	IN	denotes	of
T10925	6387-6389	NN	denotes	GC
T10926	6390-6395	NNS	denotes	pairs
T10927	6396-6397	-LRB-	denotes	(
T10929	6397-6399	CD	denotes	10
T10928	6400-6407	NNS	denotes	repeats
T10930	6408-6410	IN	denotes	of
T10931	6411-6414	NN	denotes	CGG
T10932	6414-6415	-RRB-	denotes	)
T10933	6416-6418	IN	denotes	on
T10934	6419-6420	DT	denotes	a
T10936	6421-6422	NN	denotes	Y
T10937	6422-6423	HYPH	denotes	-
T10935	6423-6427	NN	denotes	fork
T10938	6428-6431	VBD	denotes	was
T10939	6432-6435	RB	denotes	not
T10921	6436-6445	VBN	denotes	displaced
T10940	6446-6448	RB	denotes	at
T10941	6449-6452	RB	denotes	all
T10942	6452-6454	,	denotes	,
T10943	6454-6459	IN	denotes	while
T10945	6460-6463	DT	denotes	the
T10947	6464-6468	JJ	denotes	same
T10946	6469-6477	NN	denotes	template
T10948	6478-6481	VBD	denotes	was
T10949	6482-6489	RB	denotes	readily
T10944	6490-6499	VBN	denotes	displaced
T10950	6500-6502	IN	denotes	by
T10951	6503-6507	NN	denotes	SV40
T10953	6508-6509	NN	denotes	T
T10954	6509-6510	HYPH	denotes	-
T10952	6510-6517	NN	denotes	antigen
T10955	6518-6519	-LRB-	denotes	(
T10957	6519-6525	NN	denotes	Figure
T10956	6526-6528	NN	denotes	8B
T10958	6528-6529	-RRB-	denotes	)
T10959	6529-6530	.	denotes	.
T10960	6530-6666	sentence	denotes	Mcm helicase was inhibited by the presence of GC-rich duplex segment also on a circular single-stranded partial heteroduplex substrate.
T10961	6531-6534	NN	denotes	Mcm
T10962	6535-6543	NN	denotes	helicase
T10964	6544-6547	VBD	denotes	was
T10963	6548-6557	VBN	denotes	inhibited
T10965	6558-6560	IN	denotes	by
T10966	6561-6564	DT	denotes	the
T10967	6565-6573	NN	denotes	presence
T10968	6574-6576	IN	denotes	of
T10969	6577-6579	NN	denotes	GC
T10971	6579-6580	HYPH	denotes	-
T10970	6580-6584	JJ	denotes	rich
T10973	6585-6591	NN	denotes	duplex
T10972	6592-6599	NN	denotes	segment
T10974	6600-6604	RB	denotes	also
T10975	6605-6607	IN	denotes	on
T10976	6608-6609	DT	denotes	a
T10978	6610-6618	JJ	denotes	circular
T10979	6619-6625	JJ	denotes	single
T10981	6625-6626	HYPH	denotes	-
T10980	6626-6634	VBN	denotes	stranded
T10982	6635-6642	JJ	denotes	partial
T10983	6643-6655	NN	denotes	heteroduplex
T10977	6656-6665	NN	denotes	substrate
T10984	6665-6666	.	denotes	.
T10985	6666-6824	sentence	denotes	However, on this substrate, Mcm4/6/7 was able to displace DNA past the GC-rich region, albeit to a limited extent, when it was added at a high concentration.
T10986	6667-6674	RB	denotes	However
T10988	6674-6676	,	denotes	,
T10989	6676-6678	IN	denotes	on
T10990	6679-6683	DT	denotes	this
T10991	6684-6693	NN	denotes	substrate
T10992	6693-6695	,	denotes	,
T10993	6695-6699	NN	denotes	Mcm4
T10994	6699-6700	HYPH	denotes	/
T10995	6700-6701	CD	denotes	6
T10996	6701-6702	HYPH	denotes	/
T10997	6702-6703	CD	denotes	7
T10987	6704-6707	VBD	denotes	was
T10998	6708-6712	JJ	denotes	able
T10999	6713-6715	TO	denotes	to
T11000	6716-6724	VB	denotes	displace
T11001	6725-6728	NN	denotes	DNA
T11002	6729-6733	IN	denotes	past
T11003	6734-6737	DT	denotes	the
T11005	6738-6740	NN	denotes	GC
T11007	6740-6741	HYPH	denotes	-
T11006	6741-6745	JJ	denotes	rich
T11004	6746-6752	NN	denotes	region
T11008	6752-6754	,	denotes	,
T11009	6754-6760	IN	denotes	albeit
T11010	6761-6763	IN	denotes	to
T11011	6764-6765	DT	denotes	a
T11013	6766-6773	VBN	denotes	limited
T11012	6774-6780	NN	denotes	extent
T11014	6780-6782	,	denotes	,
T11015	6782-6786	WRB	denotes	when
T11017	6787-6789	PRP	denotes	it
T11018	6790-6793	VBD	denotes	was
T11016	6794-6799	VBN	denotes	added
T11019	6800-6802	IN	denotes	at
T11020	6803-6804	DT	denotes	a
T11022	6805-6809	JJ	denotes	high
T11021	6810-6823	NN	denotes	concentration
T11023	6823-6824	.	denotes	.
T11024	6824-6991	sentence	denotes	On the partial heteroduplex template, Mcm is loaded onto the circular single-stranded DNA of 6.4 kb, while it is loaded onto the 50 nt long 3′-tail DNA on the Y-fork.
T11025	6825-6827	IN	denotes	On
T11027	6828-6831	DT	denotes	the
T11029	6832-6839	JJ	denotes	partial
T11030	6840-6852	NN	denotes	heteroduplex
T11028	6853-6861	NN	denotes	template
T11031	6861-6863	,	denotes	,
T11032	6863-6866	NN	denotes	Mcm
T11033	6867-6869	VBZ	denotes	is
T11026	6870-6876	VBN	denotes	loaded
T11034	6877-6881	IN	denotes	onto
T11035	6882-6885	DT	denotes	the
T11037	6886-6894	JJ	denotes	circular
T11038	6895-6901	JJ	denotes	single
T11040	6901-6902	HYPH	denotes	-
T11039	6902-6910	VBN	denotes	stranded
T11036	6911-6914	NN	denotes	DNA
T11041	6915-6917	IN	denotes	of
T11042	6918-6921	CD	denotes	6.4
T11043	6922-6924	NN	denotes	kb
T11044	6924-6926	,	denotes	,
T11045	6926-6931	IN	denotes	while
T11047	6932-6934	PRP	denotes	it
T11048	6935-6937	VBZ	denotes	is
T11046	6938-6944	VBN	denotes	loaded
T11049	6945-6949	IN	denotes	onto
T11050	6950-6953	DT	denotes	the
T11052	6954-6956	CD	denotes	50
T11053	6957-6959	NN	denotes	nt
T11054	6960-6964	JJ	denotes	long
T11055	6965-6966	CD	denotes	3
T11057	6966-6967	SYM	denotes	′
T11058	6967-6968	HYPH	denotes	-
T11056	6968-6972	NN	denotes	tail
T11051	6973-6976	NN	denotes	DNA
T11059	6977-6979	IN	denotes	on
T11060	6980-6983	DT	denotes	the
T11062	6984-6985	NN	denotes	Y
T11063	6985-6986	HYPH	denotes	-
T11061	6986-6990	NN	denotes	fork
T11064	6990-6991	.	denotes	.
T11065	6991-7075	sentence	denotes	Thus, the difference of helicase actions may reflect the efficiency of Mcm loading.
T11066	6992-6996	RB	denotes	Thus
T11068	6996-6998	,	denotes	,
T11069	6998-7001	DT	denotes	the
T11070	7002-7012	NN	denotes	difference
T11071	7013-7015	IN	denotes	of
T11072	7016-7024	NN	denotes	helicase
T11073	7025-7032	NNS	denotes	actions
T11074	7033-7036	MD	denotes	may
T11067	7037-7044	VB	denotes	reflect
T11075	7045-7048	DT	denotes	the
T11076	7049-7059	NN	denotes	efficiency
T11077	7060-7062	IN	denotes	of
T11078	7063-7066	NN	denotes	Mcm
T11079	7067-7074	NN	denotes	loading
T11080	7074-7075	.	denotes	.
T11081	7075-7292	sentence	denotes	Alternatively, the presence of ‘random’ sequence at the initially unwound duplex segment in the former template may engage the Mcm helicase in a more active conformation which can displace the GC-rich duplex segment.
T11082	7076-7089	RB	denotes	Alternatively
T11084	7089-7091	,	denotes	,
T11085	7091-7094	DT	denotes	the
T11086	7095-7103	NN	denotes	presence
T11087	7104-7106	IN	denotes	of
T11088	7107-7108	``	denotes	‘
T11089	7108-7114	JJ	denotes	random
T11091	7114-7115	''	denotes	’
T11090	7116-7124	NN	denotes	sequence
T11092	7125-7127	IN	denotes	at
T11093	7128-7131	DT	denotes	the
T11095	7132-7141	RB	denotes	initially
T11096	7142-7149	JJ	denotes	unwound
T11097	7150-7156	NN	denotes	duplex
T11094	7157-7164	NN	denotes	segment
T11098	7165-7167	IN	denotes	in
T11099	7168-7171	DT	denotes	the
T11101	7172-7178	JJ	denotes	former
T11100	7179-7187	NN	denotes	template
T11102	7188-7191	MD	denotes	may
T11083	7192-7198	VB	denotes	engage
T11103	7199-7202	DT	denotes	the
T11105	7203-7206	NN	denotes	Mcm
T11104	7207-7215	NN	denotes	helicase
T11106	7216-7218	IN	denotes	in
T11107	7219-7220	DT	denotes	a
T11109	7221-7225	RBR	denotes	more
T11110	7226-7232	JJ	denotes	active
T11108	7233-7245	NN	denotes	conformation
T11111	7246-7251	WDT	denotes	which
T11113	7252-7255	MD	denotes	can
T11112	7256-7264	VB	denotes	displace
T11114	7265-7268	DT	denotes	the
T11116	7269-7271	NN	denotes	GC
T11118	7271-7272	HYPH	denotes	-
T11117	7272-7276	JJ	denotes	rich
T11119	7277-7283	NN	denotes	duplex
T11115	7284-7291	NN	denotes	segment
T11120	7291-7292	.	denotes	.
T11121	7292-7398	sentence	denotes	On Y-fork substrates, increase of AT base pairs in the duplex (10 repeats of CTT) restored the unwinding.
T11122	7293-7295	IN	denotes	On
T11124	7296-7297	NN	denotes	Y
T11126	7297-7298	HYPH	denotes	-
T11125	7298-7302	NN	denotes	fork
T11127	7303-7313	NNS	denotes	substrates
T11128	7313-7315	,	denotes	,
T11129	7315-7323	NN	denotes	increase
T11130	7324-7326	IN	denotes	of
T11131	7327-7329	NN	denotes	AT
T11133	7330-7334	NN	denotes	base
T11132	7335-7340	NNS	denotes	pairs
T11134	7341-7343	IN	denotes	in
T11135	7344-7347	DT	denotes	the
T11136	7348-7354	NN	denotes	duplex
T11137	7355-7356	-LRB-	denotes	(
T11139	7356-7358	CD	denotes	10
T11138	7359-7366	NNS	denotes	repeats
T11140	7367-7369	IN	denotes	of
T11141	7370-7373	NN	denotes	CTT
T11142	7373-7374	-RRB-	denotes	)
T11123	7375-7383	VBD	denotes	restored
T11143	7384-7387	DT	denotes	the
T11144	7388-7397	NN	denotes	unwinding
T11145	7397-7398	.	denotes	.
T11146	7398-7586	sentence	denotes	These results indicate either that thymine residues are required on the duplex DNA for continuous unwinding, or that Mcm4/6/7 is simply not efficient enough to unwind the stable GC pairs.
T11147	7399-7404	DT	denotes	These
T11148	7405-7412	NNS	denotes	results
T11149	7413-7421	VBP	denotes	indicate
T11150	7422-7428	CC	denotes	either
T11152	7429-7433	IN	denotes	that
T11153	7434-7441	NN	denotes	thymine
T11154	7442-7450	NNS	denotes	residues
T11155	7451-7454	VBP	denotes	are
T11151	7455-7463	VBN	denotes	required
T11156	7464-7466	IN	denotes	on
T11157	7467-7470	DT	denotes	the
T11159	7471-7477	NN	denotes	duplex
T11158	7478-7481	NN	denotes	DNA
T11160	7482-7485	IN	denotes	for
T11161	7486-7496	JJ	denotes	continuous
T11162	7497-7506	NN	denotes	unwinding
T11163	7506-7508	,	denotes	,
T11164	7508-7510	CC	denotes	or
T11165	7511-7515	IN	denotes	that
T11167	7516-7520	NN	denotes	Mcm4
T11168	7520-7521	HYPH	denotes	/
T11169	7521-7522	CD	denotes	6
T11170	7522-7523	HYPH	denotes	/
T11171	7523-7524	CD	denotes	7
T11166	7525-7527	VBZ	denotes	is
T11172	7528-7534	RB	denotes	simply
T11173	7535-7538	RB	denotes	not
T11174	7539-7548	JJ	denotes	efficient
T11175	7549-7555	RB	denotes	enough
T11176	7556-7558	TO	denotes	to
T11177	7559-7565	VB	denotes	unwind
T11178	7566-7569	DT	denotes	the
T11180	7570-7576	JJ	denotes	stable
T11181	7577-7579	NN	denotes	GC
T11179	7580-7585	NNS	denotes	pairs
T11182	7585-7586	.	denotes	.
T11183	7586-7908	sentence	denotes	Replacement of the central guanosine with inosine in the CGG repeat duplex DNA resulted in displacement (Figure 8E) (31), suggesting that the continuous presence of AT base pair may not be essential for the Mcm helicase function and that the thermostability of GC base pairs is inhibitory for helicase action of Mcm4/6/7.
T11184	7587-7598	NN	denotes	Replacement
T11186	7599-7601	IN	denotes	of
T11187	7602-7605	DT	denotes	the
T11189	7606-7613	JJ	denotes	central
T11188	7614-7623	NN	denotes	guanosine
T11190	7624-7628	IN	denotes	with
T11191	7629-7636	NN	denotes	inosine
T11192	7637-7639	IN	denotes	in
T11193	7640-7643	DT	denotes	the
T11195	7644-7647	NN	denotes	CGG
T11196	7648-7654	NN	denotes	repeat
T11197	7655-7661	NN	denotes	duplex
T11194	7662-7665	NN	denotes	DNA
T11185	7666-7674	VBD	denotes	resulted
T11198	7675-7677	IN	denotes	in
T11199	7678-7690	NN	denotes	displacement
T11200	7691-7692	-LRB-	denotes	(
T11202	7692-7698	NN	denotes	Figure
T11201	7699-7701	NN	denotes	8E
T11203	7701-7702	-RRB-	denotes	)
T11204	7703-7704	-LRB-	denotes	(
T11205	7704-7706	CD	denotes	31
T11206	7706-7707	-RRB-	denotes	)
T11207	7707-7709	,	denotes	,
T11208	7709-7719	VBG	denotes	suggesting
T11209	7720-7724	IN	denotes	that
T11211	7725-7728	DT	denotes	the
T11213	7729-7739	JJ	denotes	continuous
T11212	7740-7748	NN	denotes	presence
T11214	7749-7751	IN	denotes	of
T11215	7752-7754	NN	denotes	AT
T11217	7755-7759	NN	denotes	base
T11216	7760-7764	NN	denotes	pair
T11218	7765-7768	MD	denotes	may
T11219	7769-7772	RB	denotes	not
T11210	7773-7775	VB	denotes	be
T11220	7776-7785	JJ	denotes	essential
T11221	7786-7789	IN	denotes	for
T11222	7790-7793	DT	denotes	the
T11224	7794-7797	NN	denotes	Mcm
T11225	7798-7806	NN	denotes	helicase
T11223	7807-7815	NN	denotes	function
T11226	7816-7819	CC	denotes	and
T11227	7820-7824	IN	denotes	that
T11229	7825-7828	DT	denotes	the
T11230	7829-7844	NN	denotes	thermostability
T11231	7845-7847	IN	denotes	of
T11232	7848-7850	NN	denotes	GC
T11234	7851-7855	NN	denotes	base
T11233	7856-7861	NNS	denotes	pairs
T11228	7862-7864	VBZ	denotes	is
T11235	7865-7875	JJ	denotes	inhibitory
T11236	7876-7879	IN	denotes	for
T11237	7880-7888	NN	denotes	helicase
T11238	7889-7895	NN	denotes	action
T11239	7896-7898	IN	denotes	of
T11240	7899-7903	NN	denotes	Mcm4
T11241	7903-7904	HYPH	denotes	/
T11242	7904-7905	CD	denotes	6
T11243	7905-7906	HYPH	denotes	/
T11244	7906-7907	CD	denotes	7
T11245	7907-7908	.	denotes	.
T11246	7908-8097	sentence	denotes	The results described in this manuscript reveal potentially important features of mammalian Mcm4/6/7 helicase, which is likely to be a key component of the eukaryotic replicative helicase.
T11247	7909-7912	DT	denotes	The
T11248	7913-7920	NNS	denotes	results
T11250	7921-7930	VBN	denotes	described
T11251	7931-7933	IN	denotes	in
T11252	7934-7938	DT	denotes	this
T11253	7939-7949	NN	denotes	manuscript
T11249	7950-7956	VBP	denotes	reveal
T11254	7957-7968	RB	denotes	potentially
T11255	7969-7978	JJ	denotes	important
T11256	7979-7987	NNS	denotes	features
T11257	7988-7990	IN	denotes	of
T11258	7991-8000	JJ	denotes	mammalian
T11260	8001-8005	NN	denotes	Mcm4
T11261	8005-8006	HYPH	denotes	/
T11262	8006-8007	CD	denotes	6
T11263	8007-8008	HYPH	denotes	/
T11264	8008-8009	CD	denotes	7
T11259	8010-8018	NN	denotes	helicase
T11265	8018-8020	,	denotes	,
T11266	8020-8025	WDT	denotes	which
T11267	8026-8028	VBZ	denotes	is
T11268	8029-8035	JJ	denotes	likely
T11269	8036-8038	TO	denotes	to
T11270	8039-8041	VB	denotes	be
T11271	8042-8043	DT	denotes	a
T11273	8044-8047	JJ	denotes	key
T11272	8048-8057	NN	denotes	component
T11274	8058-8060	IN	denotes	of
T11275	8061-8064	DT	denotes	the
T11277	8065-8075	JJ	denotes	eukaryotic
T11278	8076-8087	JJ	denotes	replicative
T11276	8088-8096	NN	denotes	helicase
T11279	8096-8097	.	denotes	.
T11280	8097-8361	sentence	denotes	Prior to initiation of DNA replication, Mcm helicase may adopt a double hexameric complex at the partially melted origin region, and may catalyze concurrent unwinding of duplex DNA into both directions, while stably associated with the origins of DNA replication.
T11281	8098-8103	JJ	denotes	Prior
T11282	8104-8106	IN	denotes	to
T11284	8107-8117	NN	denotes	initiation
T11285	8118-8120	IN	denotes	of
T11286	8121-8124	NN	denotes	DNA
T11287	8125-8136	NN	denotes	replication
T11288	8136-8138	,	denotes	,
T11289	8138-8141	NN	denotes	Mcm
T11290	8142-8150	NN	denotes	helicase
T11291	8151-8154	MD	denotes	may
T11283	8155-8160	VB	denotes	adopt
T11292	8161-8162	DT	denotes	a
T11294	8163-8169	JJ	denotes	double
T11295	8170-8179	JJ	denotes	hexameric
T11293	8180-8187	NN	denotes	complex
T11296	8188-8190	IN	denotes	at
T11297	8191-8194	DT	denotes	the
T11299	8195-8204	RB	denotes	partially
T11300	8205-8211	VBN	denotes	melted
T11301	8212-8218	NN	denotes	origin
T11298	8219-8225	NN	denotes	region
T11302	8225-8227	,	denotes	,
T11303	8227-8230	CC	denotes	and
T11304	8231-8234	MD	denotes	may
T11305	8235-8243	VB	denotes	catalyze
T11306	8244-8254	JJ	denotes	concurrent
T11307	8255-8264	NN	denotes	unwinding
T11308	8265-8267	IN	denotes	of
T11309	8268-8274	NN	denotes	duplex
T11310	8275-8278	NN	denotes	DNA
T11311	8279-8283	IN	denotes	into
T11312	8284-8288	DT	denotes	both
T11313	8289-8299	NNS	denotes	directions
T11314	8299-8301	,	denotes	,
T11315	8301-8306	IN	denotes	while
T11317	8307-8313	RB	denotes	stably
T11316	8314-8324	VBN	denotes	associated
T11318	8325-8329	IN	denotes	with
T11319	8330-8333	DT	denotes	the
T11320	8334-8341	NNS	denotes	origins
T11321	8342-8344	IN	denotes	of
T11322	8345-8348	NN	denotes	DNA
T11323	8349-8360	NN	denotes	replication
T11324	8360-8361	.	denotes	.
T11325	8361-8451	sentence	denotes	This is similar to the T-antigen model originally proposed by Smelkova and Borowiec (21).
T11326	8362-8366	DT	denotes	This
T11327	8367-8369	VBZ	denotes	is
T11328	8370-8377	JJ	denotes	similar
T11329	8378-8380	IN	denotes	to
T11330	8381-8384	DT	denotes	the
T11332	8385-8386	NN	denotes	T
T11334	8386-8387	HYPH	denotes	-
T11333	8387-8394	NN	denotes	antigen
T11331	8395-8400	NN	denotes	model
T11335	8401-8411	RB	denotes	originally
T11336	8412-8420	VBN	denotes	proposed
T11337	8421-8423	IN	denotes	by
T11338	8424-8432	NNP	denotes	Smelkova
T11339	8433-8436	CC	denotes	and
T11340	8437-8445	NNP	denotes	Borowiec
T11341	8446-8447	-LRB-	denotes	(
T11342	8447-8449	CD	denotes	21
T11343	8449-8450	-RRB-	denotes	)
T11344	8450-8451	.	denotes	.
T11345	8451-8640	sentence	denotes	We propose that, only when both hexamers are activated by the interacting thymine-rich sequences present within the melted region, the Mcm helicase is mobilized and initiation takes place.
T11346	8452-8454	PRP	denotes	We
T11347	8455-8462	VBP	denotes	propose
T11348	8463-8467	IN	denotes	that
T11350	8467-8469	,	denotes	,
T11351	8469-8473	RB	denotes	only
T11353	8474-8478	WRB	denotes	when
T11354	8479-8483	DT	denotes	both
T11355	8484-8492	NNS	denotes	hexamers
T11356	8493-8496	VBP	denotes	are
T11352	8497-8506	VBN	denotes	activated
T11357	8507-8509	IN	denotes	by
T11358	8510-8513	DT	denotes	the
T11360	8514-8525	VBG	denotes	interacting
T11361	8526-8533	NN	denotes	thymine
T11363	8533-8534	HYPH	denotes	-
T11362	8534-8538	JJ	denotes	rich
T11359	8539-8548	NNS	denotes	sequences
T11364	8549-8556	JJ	denotes	present
T11365	8557-8563	IN	denotes	within
T11366	8564-8567	DT	denotes	the
T11368	8568-8574	VBN	denotes	melted
T11367	8575-8581	NN	denotes	region
T11369	8581-8583	,	denotes	,
T11370	8583-8586	DT	denotes	the
T11372	8587-8590	NN	denotes	Mcm
T11371	8591-8599	NN	denotes	helicase
T11373	8600-8602	VBZ	denotes	is
T11349	8603-8612	VBN	denotes	mobilized
T11374	8613-8616	CC	denotes	and
T11375	8617-8627	NN	denotes	initiation
T11376	8628-8633	VBZ	denotes	takes
T11377	8634-8639	NN	denotes	place
T11378	8639-8640	.	denotes	.
T11379	8640-8769	sentence	denotes	A crucial question is how these double hexameric helicase complexes are generated and turn into active helicases at the origins.
T11380	8641-8642	DT	denotes	A
T11382	8643-8650	JJ	denotes	crucial
T11381	8651-8659	NN	denotes	question
T11383	8660-8662	VBZ	denotes	is
T11384	8663-8666	WRB	denotes	how
T11386	8667-8672	DT	denotes	these
T11388	8673-8679	RB	denotes	double
T11389	8680-8689	JJ	denotes	hexameric
T11390	8690-8698	NN	denotes	helicase
T11387	8699-8708	NNS	denotes	complexes
T11391	8709-8712	VBP	denotes	are
T11385	8713-8722	VBN	denotes	generated
T11392	8723-8726	CC	denotes	and
T11393	8727-8731	VB	denotes	turn
T11394	8732-8736	IN	denotes	into
T11395	8737-8743	JJ	denotes	active
T11396	8744-8753	NNS	denotes	helicases
T11397	8754-8756	IN	denotes	at
T11398	8757-8760	DT	denotes	the
T11399	8761-8768	NNS	denotes	origins
T11400	8768-8769	.	denotes	.
T11401	8769-9023	sentence	denotes	This process would involve melting of duplex DNA, which may be facilitated by binding of preRC components in the context of proper chromatin structures, or by other unwinding factors including a topoisomerase or a newly identified Mcm8 helicase (34–36).
T11402	8770-8774	DT	denotes	This
T11403	8775-8782	NN	denotes	process
T11405	8783-8788	MD	denotes	would
T11404	8789-8796	VB	denotes	involve
T11406	8797-8804	NN	denotes	melting
T11407	8805-8807	IN	denotes	of
T11408	8808-8814	NN	denotes	duplex
T11409	8815-8818	NN	denotes	DNA
T11410	8818-8820	,	denotes	,
T11411	8820-8825	WDT	denotes	which
T11413	8826-8829	MD	denotes	may
T11414	8830-8832	VB	denotes	be
T11412	8833-8844	VBN	denotes	facilitated
T11415	8845-8847	IN	denotes	by
T11416	8848-8855	NN	denotes	binding
T11417	8856-8858	IN	denotes	of
T11418	8859-8864	NN	denotes	preRC
T11419	8865-8875	NNS	denotes	components
T11420	8876-8878	IN	denotes	in
T11421	8879-8882	DT	denotes	the
T11422	8883-8890	NN	denotes	context
T11423	8891-8893	IN	denotes	of
T11424	8894-8900	JJ	denotes	proper
T11426	8901-8910	NN	denotes	chromatin
T11425	8911-8921	NNS	denotes	structures
T11427	8921-8923	,	denotes	,
T11428	8923-8925	CC	denotes	or
T11429	8926-8928	IN	denotes	by
T11430	8929-8934	JJ	denotes	other
T11432	8935-8944	JJ	denotes	unwinding
T11431	8945-8952	NNS	denotes	factors
T11433	8953-8962	VBG	denotes	including
T11434	8963-8964	DT	denotes	a
T11435	8965-8978	NN	denotes	topoisomerase
T11436	8979-8981	CC	denotes	or
T11437	8982-8983	DT	denotes	a
T11439	8984-8989	RB	denotes	newly
T11440	8990-9000	VBN	denotes	identified
T11441	9001-9005	NN	denotes	Mcm8
T11438	9006-9014	NN	denotes	helicase
T11442	9015-9016	-LRB-	denotes	(
T11443	9016-9018	CD	denotes	34
T11444	9018-9019	SYM	denotes	–
T11445	9019-9021	CD	denotes	36
T11446	9021-9022	-RRB-	denotes	)
T11447	9022-9023	.	denotes	.
T11448	9023-9171	sentence	denotes	It may also require association of fork-assisting proteins such as Cdc45 (37,38) as well as phosphorylation events by Cdk and Cdc7 kinases (39,40).
T11449	9024-9026	PRP	denotes	It
T11451	9027-9030	MD	denotes	may
T11452	9031-9035	RB	denotes	also
T11450	9036-9043	VB	denotes	require
T11453	9044-9055	NN	denotes	association
T11454	9056-9058	IN	denotes	of
T11455	9059-9063	NN	denotes	fork
T11457	9063-9064	HYPH	denotes	-
T11456	9064-9073	VBG	denotes	assisting
T11458	9074-9082	NN	denotes	proteins
T11459	9083-9087	JJ	denotes	such
T11460	9088-9090	IN	denotes	as
T11461	9091-9096	NN	denotes	Cdc45
T11462	9097-9098	-LRB-	denotes	(
T11464	9098-9100	CD	denotes	37
T11465	9100-9101	,	denotes	,
T11463	9101-9103	CD	denotes	38
T11466	9103-9104	-RRB-	denotes	)
T11467	9105-9107	RB	denotes	as
T11469	9108-9112	RB	denotes	well
T11468	9113-9115	IN	denotes	as
T11470	9116-9131	NN	denotes	phosphorylation
T11471	9132-9138	NNS	denotes	events
T11472	9139-9141	IN	denotes	by
T11473	9142-9145	NN	denotes	Cdk
T11475	9146-9149	CC	denotes	and
T11476	9150-9154	NN	denotes	Cdc7
T11474	9155-9162	NNS	denotes	kinases
T11477	9163-9164	-LRB-	denotes	(
T11479	9164-9166	CD	denotes	39
T11480	9166-9167	,	denotes	,
T11478	9167-9169	CD	denotes	40
T11481	9169-9170	-RRB-	denotes	)
T11482	9170-9171	.	denotes	.
T11483	9171-9268	sentence	denotes	One unexpected finding of this study is low helicase activity of Mcm4/6/7 on GC-rich duplex DNA.
T11484	9172-9175	CD	denotes	One
T11486	9176-9186	JJ	denotes	unexpected
T11485	9187-9194	NN	denotes	finding
T11488	9195-9197	IN	denotes	of
T11489	9198-9202	DT	denotes	this
T11490	9203-9208	NN	denotes	study
T11487	9209-9211	VBZ	denotes	is
T11491	9212-9215	JJ	denotes	low
T11493	9216-9224	NN	denotes	helicase
T11492	9225-9233	NN	denotes	activity
T11494	9234-9236	IN	denotes	of
T11495	9237-9241	NN	denotes	Mcm4
T11496	9241-9242	HYPH	denotes	/
T11497	9242-9243	CD	denotes	6
T11498	9243-9244	HYPH	denotes	/
T11499	9244-9245	CD	denotes	7
T11500	9246-9248	IN	denotes	on
T11501	9249-9251	NN	denotes	GC
T11503	9251-9252	HYPH	denotes	-
T11502	9252-9256	JJ	denotes	rich
T11505	9257-9263	NN	denotes	duplex
T11504	9264-9267	NN	denotes	DNA
T11506	9267-9268	.	denotes	.
T11507	9268-9453	sentence	denotes	This, in conjunction with the low processivity of Mcm4/6/7 helicase, strongly indicates that the replicative helicase at the replication forks would require more than Mcm4/6/7 complex.
T11508	9269-9273	DT	denotes	This
T11510	9273-9275	,	denotes	,
T11511	9275-9277	IN	denotes	in
T11512	9278-9289	NN	denotes	conjunction
T11513	9290-9294	IN	denotes	with
T11514	9295-9298	DT	denotes	the
T11516	9299-9302	JJ	denotes	low
T11515	9303-9315	NN	denotes	processivity
T11517	9316-9318	IN	denotes	of
T11518	9319-9323	NN	denotes	Mcm4
T11520	9323-9324	HYPH	denotes	/
T11521	9324-9325	CD	denotes	6
T11522	9325-9326	HYPH	denotes	/
T11523	9326-9327	CD	denotes	7
T11519	9328-9336	NN	denotes	helicase
T11524	9336-9338	,	denotes	,
T11525	9338-9346	RB	denotes	strongly
T11509	9347-9356	VBZ	denotes	indicates
T11526	9357-9361	IN	denotes	that
T11528	9362-9365	DT	denotes	the
T11530	9366-9377	JJ	denotes	replicative
T11529	9378-9386	NN	denotes	helicase
T11531	9387-9389	IN	denotes	at
T11532	9390-9393	DT	denotes	the
T11534	9394-9405	NN	denotes	replication
T11533	9406-9411	NNS	denotes	forks
T11535	9412-9417	MD	denotes	would
T11527	9418-9425	VB	denotes	require
T11536	9426-9430	JJR	denotes	more
T11537	9431-9435	IN	denotes	than
T11538	9436-9440	NN	denotes	Mcm4
T11540	9440-9441	HYPH	denotes	/
T11541	9441-9442	CD	denotes	6
T11542	9442-9443	HYPH	denotes	/
T11543	9443-9444	CD	denotes	7
T11539	9445-9452	NN	denotes	complex
T11544	9452-9453	.	denotes	.
T11545	9453-9792	sentence	denotes	During the processive unwinding of duplex DNA replication forks, Mcm4/6/7 may be further stimulated by interaction with other proteins, including remaining Mcm subunits, Cdc45 (37,38), GINS (41,42), DNA polymerase subunits (43), Mcm8 (35,36), etc. to become a truly processive and potent helicase capable of replicating the entire genome.
T11546	9454-9460	IN	denotes	During
T11548	9461-9464	DT	denotes	the
T11550	9465-9475	JJ	denotes	processive
T11549	9476-9485	NN	denotes	unwinding
T11551	9486-9488	IN	denotes	of
T11552	9489-9495	NN	denotes	duplex
T11554	9496-9499	NN	denotes	DNA
T11555	9500-9511	NN	denotes	replication
T11553	9512-9517	NNS	denotes	forks
T11556	9517-9519	,	denotes	,
T11557	9519-9523	NN	denotes	Mcm4
T11558	9523-9524	HYPH	denotes	/
T11559	9524-9525	CD	denotes	6
T11560	9525-9526	HYPH	denotes	/
T11561	9526-9527	CD	denotes	7
T11562	9528-9531	MD	denotes	may
T11563	9532-9534	VB	denotes	be
T11564	9535-9542	RB	denotes	further
T11547	9543-9553	VBN	denotes	stimulated
T11565	9554-9556	IN	denotes	by
T11566	9557-9568	NN	denotes	interaction
T11567	9569-9573	IN	denotes	with
T11568	9574-9579	JJ	denotes	other
T11569	9580-9588	NN	denotes	proteins
T11570	9588-9590	,	denotes	,
T11571	9590-9599	VBG	denotes	including
T11572	9600-9609	VBG	denotes	remaining
T11574	9610-9613	NN	denotes	Mcm
T11573	9614-9622	NNS	denotes	subunits
T11575	9622-9624	,	denotes	,
T11576	9624-9629	NN	denotes	Cdc45
T11577	9630-9631	-LRB-	denotes	(
T11579	9631-9633	CD	denotes	37
T11580	9633-9634	,	denotes	,
T11578	9634-9636	CD	denotes	38
T11581	9636-9637	-RRB-	denotes	)
T11582	9637-9639	,	denotes	,
T11583	9639-9643	NN	denotes	GINS
T11584	9644-9645	-LRB-	denotes	(
T11586	9645-9647	CD	denotes	41
T11587	9647-9648	,	denotes	,
T11585	9648-9650	CD	denotes	42
T11588	9650-9651	-RRB-	denotes	)
T11589	9651-9653	,	denotes	,
T11590	9653-9656	NN	denotes	DNA
T11592	9657-9667	NN	denotes	polymerase
T11591	9668-9676	NNS	denotes	subunits
T11593	9677-9678	-LRB-	denotes	(
T11594	9678-9680	CD	denotes	43
T11595	9680-9681	-RRB-	denotes	)
T11596	9681-9683	,	denotes	,
T11597	9683-9687	NN	denotes	Mcm8
T11598	9688-9689	-LRB-	denotes	(
T11600	9689-9691	CD	denotes	35
T11601	9691-9692	,	denotes	,
T11599	9692-9694	CD	denotes	36
T11602	9694-9695	-RRB-	denotes	)
T11603	9695-9697	,	denotes	,
T11604	9697-9701	FW	denotes	etc.
T11605	9702-9704	TO	denotes	to
T11606	9705-9711	VB	denotes	become
T11607	9712-9713	DT	denotes	a
T11609	9714-9719	RB	denotes	truly
T11610	9720-9730	JJ	denotes	processive
T11611	9731-9734	CC	denotes	and
T11612	9735-9741	JJ	denotes	potent
T11608	9742-9750	NN	denotes	helicase
T11613	9751-9758	JJ	denotes	capable
T11614	9759-9761	IN	denotes	of
T11615	9762-9773	VBG	denotes	replicating
T11616	9774-9777	DT	denotes	the
T11618	9778-9784	JJ	denotes	entire
T11617	9785-9791	NN	denotes	genome
T11619	9791-9792	.	denotes	.
R5896	T8790	T8791	compound	DNA,helicase
R5897	T8791	T8792	nsubj	helicase,is
R5898	T8793	T8794	det	a,factor
R5899	T8794	T8792	attr	factor,is
R5900	T8795	T8794	amod	central,factor
R5901	T8796	T8794	prep	for,factor
R5902	T8797	T8796	pobj	operation,for
R5903	T8798	T8797	prep	of,operation
R5904	T8799	T8800	compound	replication,forks
R5905	T8800	T8798	pobj	forks,of
R5906	T8801	T8792	punct	.,is
R5907	T8803	T8804	nsubj	It,plays
R5908	T8805	T8804	advmod	also,plays
R5909	T8806	T8807	det	a,role
R5910	T8807	T8804	dobj	role,plays
R5911	T8808	T8807	amod	crucial,role
R5912	T8809	T8804	prep	in,plays
R5913	T8810	T8811	det	the,step
R5914	T8811	T8809	pobj	step,in
R5915	T8812	T8811	compound	initiation,step
R5916	T8813	T8804	prep	at,plays
R5917	T8814	T8815	det	the,origins
R5918	T8815	T8813	pobj	origins,at
R5919	T8816	T8815	compound	replication,origins
R5920	T8817	T8804	punct	.,plays
R5921	T8819	T8820	advmod	Therefore,is
R5922	T8821	T8820	punct	", ",is
R5923	T8822	T8823	det	the,elucidation
R5924	T8823	T8820	nsubj	elucidation,is
R5925	T8824	T8823	prep	of,elucidation
R5926	T8825	T8826	advmod	how,interact
R5927	T8826	T8824	pobj	interact,of
R5928	T8827	T8828	det	the,helicases
R5929	T8828	T8826	nsubj	helicases,interact
R5930	T8829	T8828	amod	replicative,helicases
R5931	T8830	T8826	prep	with,interact
R5932	T8831	T8832	compound	DNA,substrate
R5933	T8832	T8830	pobj	substrate,with
R5934	T8833	T8826	cc	and,interact
R5935	T8834	T8835	advmod	how,activated
R5936	T8835	T8826	conj	activated,interact
R5937	T8836	T8835	nsubjpass	they,activated
R5938	T8837	T8835	auxpass	are,activated
R5939	T8838	T8820	acomp	crucial,is
R5940	T8839	T8838	prep	for,crucial
R5941	T8840	T8839	pcomp	understanding,for
R5942	T8841	T8842	det	the,basis
R5943	T8842	T8840	dobj	basis,understanding
R5944	T8843	T8842	amod	molecular,basis
R5945	T8844	T8842	prep	of,basis
R5946	T8845	T8844	pobj	initiation,of
R5947	T8846	T8845	prep	of,initiation
R5948	T8847	T8848	compound	DNA,replication
R5949	T8848	T8846	pobj	replication,of
R5950	T8849	T8820	punct	.,is
R5951	T8851	T8852	nsubjpass	It,believed
R5952	T8853	T8852	auxpass	is,believed
R5953	T8854	T8852	advmod	now,believed
R5954	T8855	T8856	mark	that,is
R5955	T8856	T8852	ccomp	is,believed
R5956	T8857	T8856	nsubj	Mcm,is
R5957	T8858	T8859	det	a,component
R5958	T8859	T8856	attr	component,is
R5959	T8860	T8859	amod	major,component
R5960	T8861	T8859	prep	of,component
R5961	T8862	T8863	det	the,helicase
R5962	T8863	T8861	pobj	helicase,of
R5963	T8864	T8863	amod	eukaryotic,helicase
R5964	T8865	T8863	amod	replicative,helicase
R5965	T8866	T8852	punct	.,believed
R5966	T8868	T8869	prep	In,conducted
R5967	T8870	T8871	det	this,report
R5968	T8871	T8868	pobj	report,In
R5969	T8872	T8869	punct	", ",conducted
R5970	T8873	T8869	nsubj	we,conducted
R5971	T8874	T8869	aux	have,conducted
R5972	T8875	T8876	amod	detailed,analyses
R5973	T8876	T8869	dobj	analyses,conducted
R5974	T8877	T8876	prep	on,analyses
R5975	T8878	T8879	nmod	binding,actions
R5976	T8879	T8877	pobj	actions,on
R5977	T8880	T8878	cc	and,binding
R5978	T8881	T8878	conj	helicase,binding
R5979	T8882	T8879	prep	of,actions
R5980	T8883	T8884	compound	mouse,Mcm4
R5981	T8884	T8882	pobj	Mcm4,of
R5982	T8885	T8884	punct	/,Mcm4
R5983	T8886	T8884	nummod	6,Mcm4
R5984	T8887	T8884	punct	/,Mcm4
R5985	T8888	T8884	nummod	7,Mcm4
R5986	T8889	T8869	advcl	using,conducted
R5987	T8890	T8891	amod	various,substrates
R5988	T8891	T8889	dobj	substrates,using
R5989	T8892	T8891	prep	including,substrates
R5990	T8893	T8894	nmod	fork,structures
R5991	T8894	T8892	pobj	structures,including
R5992	T8895	T8893	cc	and,fork
R5993	T8896	T8893	conj	bubble,fork
R5994	T8897	T8869	punct	.,conducted
R5997	T9089	T9088	prep	of,Modes
R5998	T9090	T9089	pobj	binding,of
R5999	T9091	T9090	cc	and,binding
R6000	T9092	T9090	conj	activation,binding
R6001	T9093	T9090	prep	of,binding
R6002	T9094	T9095	nmod	Mcm4,helicase
R6003	T9095	T9093	pobj	helicase,of
R6004	T9096	T9094	punct	/,Mcm4
R6005	T9097	T9094	nummod	6,Mcm4
R6006	T9098	T9094	punct	/,Mcm4
R6007	T9099	T9094	nummod	7,Mcm4
R6008	T9100	T9090	prep	on,binding
R6009	T9101	T9102	det	a,structure
R6010	T9102	T9100	pobj	structure,on
R6011	T9103	T9102	compound	bubble,structure
R6012	T9105	T9106	nsubjpass	Initiation,associated
R6013	T9107	T9105	prep	of,Initiation
R6014	T9108	T9109	compound	DNA,replication
R6015	T9109	T9107	pobj	replication,of
R6016	T9110	T9106	auxpass	is,associated
R6017	T9111	T9106	prep	with,associated
R6018	T9112	T9113	amod	localized,melting
R6019	T9113	T9111	pobj	melting,with
R6020	T9114	T9113	prep	of,melting
R6021	T9115	T9116	compound	duplex,DNA
R6022	T9116	T9114	pobj	DNA,of
R6023	T9117	T9113	amod	near,melting
R6024	T9118	T9119	compound	replication,origins
R6025	T9119	T9117	npadvmod	origins,near
R6026	T9120	T9106	punct	.,associated
R6027	T9122	T9123	nsubjpass	Helicases,loaded
R6028	T9124	T9123	auxpass	are,loaded
R6029	T9125	T9123	prep	onto,loaded
R6030	T9126	T9127	compound	replication,forks
R6031	T9127	T9125	pobj	forks,onto
R6032	T9128	T9123	prep	through,loaded
R6033	T9129	T9130	det	the,region
R6034	T9130	T9128	pobj	region,through
R6035	T9131	T9130	amod	melted,region
R6036	T9132	T9130	punct	", ",region
R6037	T9133	T9130	acl	induced,region
R6038	T9134	T9133	prep	by,induced
R6039	T9135	T9136	compound	initiator,binding
R6040	T9136	T9134	pobj	binding,by
R6041	T9137	T9123	punct	", ",loaded
R6042	T9138	T9123	prep	in,loaded
R6043	T9139	T9138	pobj	bacteria,in
R6044	T9140	T9141	punct	(,32
R6045	T9141	T9123	parataxis	32,loaded
R6046	T9142	T9141	punct	),32
R6047	T9143	T9123	punct	.,loaded
R6048	T9145	T9146	nsubj	We,reported
R6049	T9147	T9146	advmod	previously,reported
R6050	T9148	T9149	mark	that,loaded
R6051	T9149	T9146	ccomp	loaded,reported
R6052	T9150	T9149	nsubjpass	Mcm4,loaded
R6053	T9151	T9150	punct	/,Mcm4
R6054	T9152	T9150	nummod	6,Mcm4
R6055	T9153	T9150	punct	/,Mcm4
R6056	T9154	T9150	nummod	7,Mcm4
R6057	T9155	T9149	aux	can,loaded
R6058	T9156	T9149	auxpass	be,loaded
R6059	T9157	T9149	prep	onto,loaded
R6060	T9158	T9159	det	a,structure
R6061	T9159	T9157	pobj	structure,onto
R6062	T9160	T9161	npadvmod	bubble,like
R6063	T9161	T9159	amod	like,structure
R6064	T9162	T9161	punct	-,like
R6065	T9163	T9146	cc	and,reported
R6066	T9164	T9165	aux	can,serve
R6067	T9165	T9146	conj	serve,reported
R6068	T9166	T9165	prep	as,serve
R6069	T9167	T9168	det	a,helicase
R6070	T9168	T9166	pobj	helicase,as
R6071	T9169	T9168	compound	DNA,helicase
R6072	T9170	T9165	prep	at,serve
R6073	T9171	T9172	det	the,forks
R6074	T9172	T9170	pobj	forks,at
R6075	T9173	T9174	punct	(,13
R6076	T9174	T9165	parataxis	13,serve
R6077	T9175	T9174	punct	),13
R6078	T9176	T9146	punct	.,reported
R6079	T9178	T9179	det	The,ability
R6080	T9179	T9180	nsubj	ability,indicates
R6081	T9181	T9179	prep	of,ability
R6082	T9182	T9181	pobj	Mcm4,of
R6083	T9183	T9182	punct	/,Mcm4
R6084	T9184	T9182	nummod	6,Mcm4
R6085	T9185	T9182	punct	/,Mcm4
R6086	T9186	T9182	nummod	7,Mcm4
R6087	T9187	T9188	aux	to,unwind
R6088	T9188	T9179	acl	unwind,ability
R6089	T9189	T9190	det	the,substrate
R6090	T9190	T9188	dobj	substrate,unwind
R6091	T9191	T9190	compound	bubble,substrate
R6092	T9192	T9190	cc	but,substrate
R6093	T9193	T9192	neg	not,but
R6094	T9194	T9195	det	the,DNA
R6095	T9195	T9190	conj	DNA,substrate
R6096	T9196	T9195	amod	conventional,DNA
R6097	T9197	T9195	compound	duplex,DNA
R6098	T9198	T9199	punct	(,2
R6099	T9199	T9188	parataxis	2,unwind
R6100	T9200	T9199	nmod	Figures,2
R6101	T9201	T9199	cc	and,2
R6102	T9202	T9199	conj	13,2
R6103	T9203	T9199	punct	),2
R6104	T9204	T9205	mark	that,loaded
R6105	T9205	T9180	ccomp	loaded,indicates
R6106	T9206	T9205	nsubjpass	Mcm,loaded
R6107	T9207	T9205	aux	can,loaded
R6108	T9208	T9205	auxpass	be,loaded
R6109	T9209	T9205	prep	through,loaded
R6110	T9210	T9211	det	the,segment
R6111	T9211	T9209	pobj	segment,through
R6112	T9212	T9213	amod	single,stranded
R6113	T9213	T9211	amod	stranded,segment
R6114	T9214	T9213	punct	-,stranded
R6115	T9215	T9211	prep	of,segment
R6116	T9216	T9217	det	the,bubble
R6117	T9217	T9215	pobj	bubble,of
R6118	T9218	T9180	punct	.,indicates
R6119	T9220	T9221	advmod	Furthermore,displays
R6120	T9222	T9221	punct	", ",displays
R6121	T9223	T9224	det	the,complex
R6122	T9224	T9221	nsubj	complex,displays
R6123	T9225	T9224	nmod	Mcm4,complex
R6124	T9226	T9225	punct	/,Mcm4
R6125	T9227	T9225	nummod	6,Mcm4
R6126	T9228	T9225	punct	/,Mcm4
R6127	T9229	T9225	nummod	7,Mcm4
R6128	T9230	T9231	amod	marked,preference
R6129	T9231	T9221	dobj	preference,displays
R6130	T9232	T9231	prep	for,preference
R6131	T9233	T9234	npadvmod	thymine,rich
R6132	T9234	T9236	amod	rich,sequences
R6133	T9235	T9234	punct	-,rich
R6134	T9236	T9232	pobj	sequences,for
R6135	T9237	T9221	prep	within,displays
R6136	T9238	T9239	det	the,segment
R6137	T9239	T9237	pobj	segment,within
R6138	T9240	T9241	amod	single,stranded
R6139	T9241	T9239	amod	stranded,segment
R6140	T9242	T9241	punct	-,stranded
R6141	T9243	T9239	prep	for,segment
R6142	T9244	T9245	compound	helicase,activation
R6143	T9245	T9243	pobj	activation,for
R6144	T9246	T9247	punct	(,13
R6145	T9247	T9221	parataxis	13,displays
R6146	T9248	T9247	punct	),13
R6147	T9249	T9221	punct	.,displays
R6148	T9251	T9252	det	The,results
R6149	T9252	T9253	nsubj	results,indicate
R6150	T9254	T9252	prep	in,results
R6151	T9255	T9256	det	this,report
R6152	T9256	T9254	pobj	report,in
R6153	T9257	T9258	mark	that,depend
R6154	T9258	T9253	ccomp	depend,indicate
R6155	T9259	T9260	compound	helicase,action
R6156	T9260	T9258	nsubj	action,depend
R6157	T9261	T9260	prep	of,action
R6158	T9262	T9261	pobj	Mcm4,of
R6159	T9263	T9262	punct	/,Mcm4
R6160	T9264	T9262	nummod	6,Mcm4
R6161	T9265	T9262	punct	/,Mcm4
R6162	T9266	T9262	nummod	7,Mcm4
R6163	T9267	T9260	prep	on,action
R6164	T9268	T9269	amod	synthetic,bubbles
R6165	T9269	T9267	pobj	bubbles,on
R6166	T9270	T9258	aux	may,depend
R6167	T9271	T9258	prep	on,depend
R6168	T9272	T9273	det	the,presence
R6169	T9273	T9271	pobj	presence,on
R6170	T9274	T9273	prep	of,presence
R6171	T9275	T9276	det	an,region
R6172	T9276	T9274	pobj	region,of
R6173	T9277	T9276	amod	unpaired,region
R6174	T9278	T9276	prep	of,region
R6175	T9279	T9280	amod	sufficient,length
R6176	T9280	T9278	pobj	length,of
R6177	T9281	T9282	punct	(,nt
R6178	T9282	T9280	parataxis	nt,length
R6179	T9283	T9284	advmod	at,least
R6180	T9284	T9285	advmod	least,40
R6181	T9285	T9282	nummod	40,nt
R6182	T9286	T9282	punct	),nt
R6183	T9287	T9276	punct	", ",region
R6184	T9288	T9289	dep	which,permit
R6185	T9289	T9276	relcl	permit,region
R6186	T9290	T9289	aux	may,permit
R6187	T9291	T9289	dobj	assembly,permit
R6188	T9292	T9291	prep	of,assembly
R6189	T9293	T9294	det	a,complex
R6190	T9294	T9292	pobj	complex,of
R6191	T9295	T9296	amod	double,hexameric
R6192	T9296	T9294	amod	hexameric,complex
R6193	T9297	T9296	punct	-,hexameric
R6194	T9298	T9294	prep	on,complex
R6195	T9299	T9300	det	the,DNA
R6196	T9300	T9298	pobj	DNA,on
R6197	T9301	T9300	compound	substrate,DNA
R6198	T9302	T9294	punct	", ",complex
R6199	T9303	T9294	amod	similar,complex
R6200	T9304	T9303	prep	to,similar
R6201	T9305	T9306	compound	SV40,protein
R6202	T9306	T9304	pobj	protein,to
R6203	T9307	T9308	compound	T,antigen
R6204	T9308	T9306	compound	antigen,protein
R6205	T9309	T9308	punct	-,antigen
R6206	T9310	T9311	punct	(,2A
R6207	T9311	T9304	parataxis	2A,to
R6208	T9312	T9311	compound	Figure,2A
R6209	T9313	T9311	punct	),2A
R6210	T9314	T9315	punct	(,21
R6211	T9315	T9289	parataxis	21,permit
R6212	T9316	T9315	punct	),21
R6213	T9317	T9253	punct	.,indicate
R6214	T9319	T9320	advmod	When,replaced
R6215	T9320	T9335	advcl	replaced,reduced
R6216	T9321	T9322	nummod	one,strand
R6217	T9322	T9320	nsubjpass	strand,replaced
R6218	T9323	T9322	prep	of,strand
R6219	T9324	T9325	det	the,segment
R6220	T9325	T9323	pobj	segment,of
R6221	T9326	T9327	amod	single,stranded
R6222	T9327	T9325	amod	stranded,segment
R6223	T9328	T9327	punct	-,stranded
R6224	T9329	T9322	prep	in,strand
R6225	T9330	T9331	npadvmod	T,rich
R6226	T9331	T9333	amod	rich,bubble
R6227	T9332	T9331	punct	-,rich
R6228	T9333	T9329	pobj	bubble,in
R6229	T9334	T9320	auxpass	was,replaced
R6230	T9336	T9320	agent	by,replaced
R6231	T9337	T9338	npadvmod	guanine,rich
R6232	T9338	T9340	amod	rich,sequences
R6233	T9339	T9338	punct	-,rich
R6234	T9340	T9336	pobj	sequences,by
R6235	T9341	T9335	punct	", ",reduced
R6236	T9342	T9343	det	the,efficiency
R6237	T9343	T9335	nsubjpass	efficiency,reduced
R6238	T9344	T9343	prep	of,efficiency
R6239	T9345	T9344	pobj	unwinding,of
R6240	T9346	T9335	auxpass	was,reduced
R6241	T9347	T9335	advmod	significantly,reduced
R6242	T9348	T9335	punct	.,reduced
R6243	T9350	T9351	poss	Our,analyses
R6244	T9351	T9353	nsubj	analyses,showed
R6245	T9352	T9351	compound	footprinting,analyses
R6246	T9354	T9355	mark	that,protects
R6247	T9355	T9353	ccomp	protects,showed
R6248	T9356	T9355	nsubj	Mcm4,protects
R6249	T9357	T9356	punct	/,Mcm4
R6250	T9358	T9356	nummod	6,Mcm4
R6251	T9359	T9356	punct	/,Mcm4
R6252	T9360	T9356	nummod	7,Mcm4
R6253	T9361	T9355	advmod	strongly,protects
R6254	T9362	T9363	nummod	25,nt
R6255	T9363	T9364	nmod	nt,segment
R6256	T9364	T9355	dobj	segment,protects
R6257	T9365	T9366	amod	single,stranded
R6258	T9366	T9364	amod	stranded,segment
R6259	T9367	T9366	punct	-,stranded
R6260	T9368	T9364	compound	DNA,segment
R6261	T9369	T9364	amod	adjacent,segment
R6262	T9370	T9369	prep	to,adjacent
R6263	T9371	T9372	det	each,point
R6264	T9372	T9370	pobj	point,to
R6265	T9373	T9372	compound	branch,point
R6266	T9374	T9369	cc	and,adjacent
R6267	T9375	T9369	conj	proximal,adjacent
R6268	T9376	T9375	prep	to,proximal
R6269	T9377	T9378	nummod	5,ends
R6270	T9378	T9376	pobj	ends,to
R6271	T9379	T9377	punct	′,5
R6272	T9380	T9378	prep	of,ends
R6273	T9381	T9382	det	both,strands
R6274	T9382	T9380	pobj	strands,of
R6275	T9383	T9382	prep	of,strands
R6276	T9384	T9385	det	the,bubble
R6277	T9385	T9383	pobj	bubble,of
R6278	T9386	T9353	punct	.,showed
R6279	T9388	T9389	prep	At,is
R6280	T9390	T9391	det	each,fork
R6281	T9391	T9388	pobj	fork,At
R6282	T9392	T9389	punct	", ",is
R6283	T9393	T9394	nummod	one,complex
R6284	T9394	T9389	nsubj	complex,is
R6285	T9395	T9394	nmod	Mcm4,complex
R6286	T9396	T9395	punct	/,Mcm4
R6287	T9397	T9395	nummod	6,Mcm4
R6288	T9398	T9395	punct	/,Mcm4
R6289	T9399	T9395	nummod	7,Mcm4
R6290	T9400	T9389	acomp	likely,is
R6291	T9401	T9402	aux	to,encircle
R6292	T9402	T9400	xcomp	encircle,likely
R6293	T9403	T9404	det	the,strand
R6294	T9404	T9402	dobj	strand,encircle
R6295	T9405	T9406	amod	single,stranded
R6296	T9406	T9404	amod	stranded,strand
R6297	T9407	T9406	punct	-,stranded
R6298	T9408	T9404	compound	DNA,strand
R6299	T9409	T9389	cc	and,is
R6300	T9410	T9411	nummod	two,hexamers
R6301	T9411	T9412	nsubj	hexamers,bind
R6302	T9412	T9389	conj	bind,is
R6303	T9413	T9412	aux	may,bind
R6304	T9414	T9412	advmod	symmetrically,bind
R6305	T9415	T9412	prep	to,bind
R6306	T9416	T9417	det	the,substrate
R6307	T9417	T9415	pobj	substrate,to
R6308	T9418	T9417	compound	bubble,substrate
R6309	T9419	T9412	punct	", ",bind
R6310	T9420	T9412	advcl	forming,bind
R6311	T9421	T9422	det	a,structure
R6312	T9422	T9420	dobj	structure,forming
R6313	T9423	T9424	amod	double,hexameric
R6314	T9424	T9422	amod	hexameric,structure
R6315	T9425	T9420	prep	on,forming
R6316	T9426	T9427	det	the,bubble
R6317	T9427	T9425	pobj	bubble,on
R6318	T9428	T9429	punct	(,see
R6319	T9429	T9420	parataxis	see,forming
R6320	T9430	T9431	amod	schematic,drawing
R6321	T9431	T9429	dobj	drawing,see
R6322	T9432	T9431	prep	of,drawing
R6323	T9433	T9434	compound	Figure,1A
R6324	T9434	T9432	pobj	1A,of
R6325	T9435	T9429	punct	),see
R6326	T9436	T9412	punct	.,bind
R6327	T9438	T9439	amod	Efficient,unwinding
R6328	T9439	T9440	nsubj	unwinding,require
R6329	T9441	T9439	prep	into,unwinding
R6330	T9442	T9443	det	both,directions
R6331	T9443	T9441	pobj	directions,into
R6332	T9444	T9440	aux	may,require
R6333	T9445	T9446	amod	simultaneous,activation
R6334	T9446	T9440	dobj	activation,require
R6335	T9447	T9446	prep	of,activation
R6336	T9448	T9449	det	both,hexamers
R6337	T9449	T9447	pobj	hexamers,of
R6338	T9450	T9451	dep	which,sit
R6339	T9451	T9449	relcl	sit,hexamers
R6340	T9452	T9451	aux	may,sit
R6341	T9453	T9451	prep	at,sit
R6342	T9454	T9455	det	the,center
R6343	T9455	T9453	pobj	center,at
R6344	T9456	T9457	mark	while,extruding
R6345	T9457	T9451	advcl	extruding,sit
R6346	T9458	T9459	det	the,DNA
R6347	T9459	T9457	dobj	DNA,extruding
R6348	T9460	T9459	amod	unwound,DNA
R6349	T9461	T9462	amod	single,stranded
R6350	T9462	T9459	amod	stranded,DNA
R6351	T9463	T9462	punct	-,stranded
R6352	T9464	T9457	prep	through,extruding
R6353	T9465	T9466	det	the,rings
R6354	T9466	T9464	pobj	rings,through
R6355	T9467	T9440	punct	.,require
R6356	T9469	T9470	nsubj	This,resemble
R6357	T9471	T9470	aux	may,resemble
R6358	T9472	T9470	advmod	closely,resemble
R6359	T9473	T9474	det	the,modes
R6360	T9474	T9470	dobj	modes,resemble
R6361	T9475	T9474	amod	proposed,modes
R6362	T9476	T9474	prep	of,modes
R6363	T9477	T9478	nmod	binding,actions
R6364	T9478	T9476	pobj	actions,of
R6365	T9479	T9477	cc	and,binding
R6366	T9480	T9477	conj	helicase,binding
R6367	T9481	T9478	prep	of,actions
R6368	T9482	T9483	compound	T,antigen
R6369	T9483	T9481	pobj	antigen,of
R6370	T9484	T9483	punct	-,antigen
R6371	T9485	T9486	punct	(,21
R6372	T9486	T9470	parataxis	21,resemble
R6373	T9487	T9486	punct	),21
R6374	T9488	T9470	punct	.,resemble
R6375	T9490	T9491	mark	Although,exclude
R6376	T9491	T9496	advcl	exclude,bear
R6377	T9492	T9491	nsubj	we,exclude
R6378	T9493	T9491	aux	can,exclude
R6379	T9494	T9491	neg	not,exclude
R6380	T9495	T9491	advmod	totally,exclude
R6381	T9497	T9498	det	the,possibility
R6382	T9498	T9491	dobj	possibility,exclude
R6383	T9499	T9500	mark	that,unwinds
R6384	T9500	T9498	acl	unwinds,possibility
R6385	T9501	T9502	nummod	one,hexamer
R6386	T9502	T9500	nsubj	hexamer,unwinds
R6387	T9503	T9502	nmod	Mcm4,hexamer
R6388	T9504	T9503	punct	/,Mcm4
R6389	T9505	T9503	nummod	6,Mcm4
R6390	T9506	T9503	punct	/,Mcm4
R6391	T9507	T9503	nummod	7,Mcm4
R6392	T9508	T9502	prep	at,hexamer
R6393	T9509	T9510	det	each,fork
R6394	T9510	T9508	pobj	fork,at
R6395	T9511	T9512	det	the,duplex
R6396	T9512	T9500	dobj	duplex,unwinds
R6397	T9513	T9500	advmod	independently,unwinds
R6398	T9514	T9496	punct	", ",bear
R6399	T9515	T9516	npadvmod	ring,shaped
R6400	T9516	T9518	amod	shaped,structures
R6401	T9517	T9516	punct	-,shaped
R6402	T9518	T9496	nsubj	structures,bear
R6403	T9519	T9518	prep	of,structures
R6404	T9520	T9521	nmod	mouse,MCM
R6405	T9521	T9519	pobj	MCM,of
R6406	T9522	T9520	cc	and,mouse
R6407	T9523	T9520	conj	archea,mouse
R6408	T9524	T9496	punct	", ",bear
R6409	T9525	T9526	mark	as,revealed
R6410	T9526	T9496	advcl	revealed,bear
R6411	T9527	T9526	agent	by,revealed
R6412	T9528	T9529	compound	electron,microscopy
R6413	T9529	T9527	pobj	microscopy,by
R6414	T9530	T9496	punct	", ",bear
R6415	T9531	T9532	amod	much,similarity
R6416	T9532	T9496	dobj	similarity,bear
R6417	T9533	T9532	prep	to,similarity
R6418	T9534	T9535	det	the,structure
R6419	T9535	T9533	pobj	structure,to
R6420	T9536	T9537	advmod	recently,solved
R6421	T9537	T9535	amod	solved,structure
R6422	T9538	T9535	prep	of,structure
R6423	T9539	T9540	det	the,antigen
R6424	T9540	T9538	pobj	antigen,of
R6425	T9541	T9540	nmod	SV40,antigen
R6426	T9542	T9540	amod	large,antigen
R6427	T9543	T9540	compound	T,antigen
R6428	T9544	T9540	punct	-,antigen
R6429	T9545	T9546	punct	(,6
R6430	T9546	T9535	parataxis	6,structure
R6431	T9547	T9546	nummod	4,6
R6432	T9548	T9546	punct	",",6
R6433	T9549	T9546	punct	),6
R6434	T9550	T9496	punct	", ",bear
R6435	T9551	T9496	cc	and,bear
R6436	T9552	T9496	conj	are,bear
R6437	T9553	T9552	prep	in,are
R6438	T9554	T9553	pobj	favor,in
R6439	T9555	T9554	prep	of,favor
R6440	T9556	T9557	det	the,structure
R6441	T9557	T9555	pobj	structure,of
R6442	T9558	T9559	amod	double,hexameric
R6443	T9559	T9557	amod	hexameric,structure
R6444	T9560	T9559	punct	-,hexameric
R6445	T9561	T9557	prep	of,structure
R6446	T9562	T9563	compound	mouse,Mcm
R6447	T9563	T9561	pobj	Mcm,of
R6448	T9564	T9557	prep	on,structure
R6449	T9565	T9566	det	a,DNA
R6450	T9566	T9564	pobj	DNA,on
R6451	T9567	T9566	compound	bubble,DNA
R6452	T9568	T9496	punct	.,bear
R6455	T9856	T9857	nmod	Substrate,requirement
R6456	T9858	T9856	cc	and,Substrate
R6457	T9859	T9856	conj	sequence,Substrate
R6458	T9860	T9857	prep	for,requirement
R6459	T9861	T9862	nmod	Mcm4,activation
R6460	T9862	T9860	pobj	activation,for
R6461	T9863	T9861	punct	/,Mcm4
R6462	T9864	T9861	nummod	6,Mcm4
R6463	T9865	T9861	punct	/,Mcm4
R6464	T9866	T9861	nummod	7,Mcm4
R6465	T9867	T9862	compound	helicase,activation
R6466	T9869	T9870	compound	DNA,binding
R6467	T9870	T9871	compound	binding,assays
R6468	T9871	T9872	nsubj	assays,indicate
R6469	T9873	T9874	mark	that,binds
R6470	T9874	T9872	ccomp	binds,indicate
R6471	T9875	T9874	nsubj	Mcm4,binds
R6472	T9876	T9875	punct	/,Mcm4
R6473	T9877	T9875	nummod	6,Mcm4
R6474	T9878	T9875	punct	/,Mcm4
R6475	T9879	T9875	nummod	7,Mcm4
R6476	T9880	T9874	prep	to,binds
R6477	T9881	T9882	det	those,substrates
R6478	T9882	T9880	pobj	substrates,to
R6479	T9883	T9882	acl	containing,substrates
R6480	T9884	T9885	amod	single,stranded
R6481	T9885	T9887	amod	stranded,regions
R6482	T9886	T9885	punct	-,stranded
R6483	T9887	T9883	dobj	regions,containing
R6484	T9888	T9887	compound	DNA,regions
R6485	T9889	T9874	advmod	regardless,binds
R6486	T9890	T9889	prep	of,regardless
R6487	T9891	T9892	det	the,presence
R6488	T9892	T9890	pobj	presence,of
R6489	T9893	T9892	cc	or,presence
R6490	T9894	T9892	conj	absence,presence
R6491	T9895	T9892	prep	of,presence
R6492	T9896	T9897	amod	single,stranded
R6493	T9897	T9899	amod	stranded,end
R6494	T9898	T9897	punct	-,stranded
R6495	T9899	T9895	pobj	end,of
R6496	T9900	T9899	nummod	3,end
R6497	T9901	T9900	punct	′,3
R6498	T9902	T9900	cc	or,3
R6499	T9903	T9900	conj	5,3
R6500	T9904	T9903	punct	′,5
R6501	T9905	T9872	punct	.,indicate
R6502	T9907	T9908	nsubj	Unwinding,depends
R6503	T9909	T9907	prep	of,Unwinding
R6504	T9910	T9911	det	the,DNA
R6505	T9911	T9909	pobj	DNA,of
R6506	T9912	T9911	compound	duplex,DNA
R6507	T9913	T9908	prep	on,depends
R6508	T9914	T9913	pobj	translocation,on
R6509	T9915	T9914	prep	of,translocation
R6510	T9916	T9917	amod	single,stranded
R6511	T9917	T9919	amod	stranded,DNA
R6512	T9918	T9917	punct	-,stranded
R6513	T9919	T9915	pobj	DNA,of
R6514	T9920	T9914	prep	from,translocation
R6515	T9921	T9920	pobj	3,from
R6516	T9922	T9921	punct	′,3
R6517	T9923	T9920	prep	to,from
R6518	T9924	T9923	pobj	5,to
R6519	T9925	T9924	punct	′,5
R6520	T9926	T9920	pobj	direction,from
R6521	T9927	T9908	punct	.,depends
R6522	T9929	T9930	nsubjpass	This,shown
R6523	T9931	T9930	auxpass	is,shown
R6524	T9932	T9933	advmod	most,clearly
R6525	T9933	T9930	advmod	clearly,shown
R6526	T9934	T9930	prep	by,shown
R6527	T9935	T9936	poss	its,action
R6528	T9936	T9934	pobj	action,by
R6529	T9937	T9936	compound	helicase,action
R6530	T9938	T9936	prep	on,action
R6531	T9939	T9940	nmod	A,fork
R6532	T9940	T9942	nmod	fork,extension
R6533	T9941	T9940	punct	-,fork
R6534	T9942	T9938	pobj	extension,on
R6535	T9943	T9940	punct	[,fork
R6536	T9944	T9940	appos	5,fork
R6537	T9945	T9944	punct	′,5
R6538	T9946	T9944	punct	],5
R6539	T9947	T9944	cc	and,5
R6540	T9948	T9944	conj	3,5
R6541	T9949	T9948	punct	′,3
R6542	T9950	T9942	punct	-,extension
R6543	T9951	T9938	cc	but,on
R6544	T9952	T9951	neg	not,but
R6545	T9953	T9938	conj	on,on
R6546	T9954	T9955	nmod	A,fork
R6547	T9955	T9957	nmod	fork,extension
R6548	T9956	T9955	punct	-,fork
R6549	T9957	T9953	pobj	extension,on
R6550	T9958	T9955	punct	[,fork
R6551	T9959	T9955	nummod	3,fork
R6552	T9960	T9955	punct	′,fork
R6553	T9961	T9955	punct	],fork
R6554	T9962	T9955	punct	", ",fork
R6555	T9963	T9964	compound	A,fork
R6556	T9964	T9955	conj	fork,fork
R6557	T9965	T9964	punct	-,fork
R6558	T9966	T9964	punct	[,fork
R6559	T9967	T9964	appos	3,fork
R6560	T9968	T9967	punct	′,3
R6561	T9969	T9967	punct	",",3
R6562	T9970	T9967	appos	5,3
R6563	T9971	T9970	punct	′,5
R6564	T9972	T9964	punct	],fork
R6565	T9973	T9964	cc	nor,fork
R6566	T9974	T9964	conj	5,fork
R6567	T9975	T9974	punct	′,5
R6568	T9976	T9957	punct	-,extension
R6569	T9977	T9930	punct	.,shown
R6570	T9979	T9980	advmod	However,require
R6571	T9981	T9980	punct	", ",require
R6572	T9982	T9980	nsubj	it,require
R6573	T9983	T9980	aux	does,require
R6574	T9984	T9980	neg	not,require
R6575	T9985	T9980	advmod	necessarily,require
R6576	T9986	T9987	nummod	3,end
R6577	T9987	T9980	dobj	end,require
R6578	T9988	T9986	punct	′,3
R6579	T9989	T9987	prep	of,end
R6580	T9990	T9991	amod	single,stranded
R6581	T9991	T9993	amod	stranded,DNA
R6582	T9992	T9991	punct	-,stranded
R6583	T9993	T9989	pobj	DNA,of
R6584	T9994	T9980	punct	", ",require
R6585	T9995	T9996	mark	since,displace
R6586	T9996	T9980	advcl	displace,require
R6587	T9997	T9996	nsubj	Mcm4,displace
R6588	T9998	T9997	punct	/,Mcm4
R6589	T9999	T9997	nummod	6,Mcm4
R6590	T10000	T9997	punct	/,Mcm4
R6591	T10001	T9997	nummod	7,Mcm4
R6592	T10002	T9996	aux	can,displace
R6593	T10003	T10004	amod	annealing,oligonucleotide
R6594	T10004	T9996	dobj	oligonucleotide,displace
R6595	T10005	T9996	prep	on,displace
R6596	T10006	T10007	det	a,DNA
R6597	T10007	T10005	pobj	DNA,on
R6598	T10008	T10007	amod	circular,DNA
R6599	T10009	T10010	amod	single,stranded
R6600	T10010	T10007	amod	stranded,DNA
R6601	T10011	T10010	punct	-,stranded
R6602	T10012	T9980	punct	.,require
R6603	T10014	T10015	det	The,ability
R6604	T10015	T10016	nsubjpass	ability,shared
R6605	T10017	T10015	prep	of,ability
R6606	T10018	T10019	det	the,Mcm4
R6607	T10019	T10017	pobj	Mcm4,of
R6608	T10020	T10019	compound	mouse,Mcm4
R6609	T10021	T10019	punct	/,Mcm4
R6610	T10022	T10019	nummod	6,Mcm4
R6611	T10023	T10019	punct	/,Mcm4
R6612	T10024	T10019	nummod	7,Mcm4
R6613	T10025	T10026	aux	to,unwind
R6614	T10026	T10015	acl	unwind,ability
R6615	T10027	T10028	nummod	3,extension
R6616	T10028	T10026	dobj	extension,unwind
R6617	T10029	T10027	punct	′,3
R6618	T10030	T10028	punct	-,extension
R6619	T10031	T10016	auxpass	is,shared
R6620	T10032	T10016	prep	by,shared
R6621	T10033	T10034	det	the,helicase
R6622	T10034	T10032	pobj	helicase,by
R6623	T10035	T10034	amod	archaeal,helicase
R6624	T10036	T10034	compound	Mcm,helicase
R6625	T10037	T10032	cc	but,by
R6626	T10038	T10037	neg	not,but
R6627	T10039	T10032	conj	by,by
R6628	T10040	T10039	pobj	Mcm4,by
R6629	T10041	T10040	punct	/,Mcm4
R6630	T10042	T10040	nummod	6,Mcm4
R6631	T10043	T10040	punct	/,Mcm4
R6632	T10044	T10040	nummod	7,Mcm4
R6633	T10045	T10040	prep	from,Mcm4
R6634	T10046	T10045	pobj	S.pombe,from
R6635	T10047	T10046	cc	and,S.pombe
R6636	T10048	T10046	conj	S.cerevisiae,S.pombe
R6637	T10049	T10050	punct	(,27
R6638	T10050	T10016	parataxis	27,shared
R6639	T10051	T10050	nummod	14,27
R6640	T10052	T10050	punct	",",27
R6641	T10053	T10050	nummod	26,27
R6642	T10054	T10050	punct	",",27
R6643	T10055	T10050	punct	),27
R6644	T10056	T10016	punct	.,shared
R6645	T10058	T10059	det	The,Mcm
R6646	T10059	T10061	nsubj	Mcm,unwind
R6647	T10060	T10059	amod	archaeal,Mcm
R6648	T10062	T10061	aux	can,unwind
R6649	T10063	T10064	compound	A,fork
R6650	T10064	T10061	dobj	fork,unwind
R6651	T10065	T10064	punct	-,fork
R6652	T10066	T10064	punct	[,fork
R6653	T10067	T10064	nummod	3,fork
R6654	T10068	T10064	punct	′,fork
R6655	T10069	T10061	punct	],unwind
R6656	T10070	T10061	cc	but,unwind
R6657	T10071	T10072	amod	eukaryotic,Mcm4
R6658	T10072	T10073	nsubj	Mcm4,can
R6659	T10073	T10061	conj	can,unwind
R6660	T10074	T10072	punct	/,Mcm4
R6661	T10075	T10072	nummod	6,Mcm4
R6662	T10076	T10072	punct	/,Mcm4
R6663	T10077	T10072	nummod	7,Mcm4
R6664	T10078	T10073	neg	not,can
R6665	T10079	T10061	punct	", ",unwind
R6666	T10080	T10081	mark	since,binds
R6667	T10081	T10061	advcl	binds,unwind
R6668	T10082	T10083	det	the,former
R6669	T10083	T10081	nsubj	former,binds
R6670	T10084	T10081	prep	to,binds
R6671	T10085	T10086	amod	double,stranded
R6672	T10086	T10088	amod	stranded,DNA
R6673	T10087	T10086	punct	-,stranded
R6674	T10088	T10084	pobj	DNA,to
R6675	T10089	T10081	cc	but,binds
R6676	T10090	T10091	det	the,latter
R6677	T10091	T10092	nsubj	latter,27
R6678	T10092	T10081	conj	27,binds
R6679	T10093	T10092	aux	does,27
R6680	T10094	T10092	neg	not,27
R6681	T10095	T10092	punct	(,27
R6682	T10096	T10092	nummod	5,27
R6683	T10097	T10092	punct	",",27
R6684	T10098	T10092	punct	),27
R6685	T10099	T10061	punct	.,unwind
R6686	T10101	T10102	mark	While,translocate
R6687	T10102	T10107	advcl	translocate,observed
R6688	T10103	T10104	compound	yeast,Mcm
R6689	T10104	T10105	compound	Mcm,helicases
R6690	T10105	T10102	nsubj	helicases,translocate
R6691	T10106	T10102	aux	can,translocate
R6692	T10108	T10102	prep	on,translocate
R6693	T10109	T10110	compound	duplex,DNA
R6694	T10110	T10108	pobj	DNA,on
R6695	T10111	T10107	punct	", ",observed
R6696	T10112	T10113	amod	such,activity
R6697	T10113	T10107	nsubjpass	activity,observed
R6698	T10114	T10107	auxpass	was,observed
R6699	T10115	T10107	neg	not,observed
R6700	T10116	T10107	prep	with,observed
R6701	T10117	T10118	amod	mammalian,Mcm4
R6702	T10118	T10116	pobj	Mcm4,with
R6703	T10119	T10118	punct	/,Mcm4
R6704	T10120	T10118	nummod	6,Mcm4
R6705	T10121	T10118	punct	/,Mcm4
R6706	T10122	T10118	nummod	7,Mcm4
R6707	T10123	T10124	punct	(,shown
R6708	T10124	T10107	parataxis	shown,observed
R6709	T10125	T10124	nsubj	data,shown
R6710	T10126	T10124	neg	not,shown
R6711	T10127	T10124	punct	),shown
R6712	T10128	T10107	punct	.,observed
R6713	T10130	T10131	nsubj	Occurrence,lead
R6714	T10132	T10130	prep	of,Occurrence
R6715	T10133	T10134	npadvmod	AT,rich
R6716	T10134	T10136	amod	rich,sequences
R6717	T10135	T10134	punct	-,rich
R6718	T10136	T10132	pobj	sequences,of
R6719	T10137	T10130	punct	", ",Occurrence
R6720	T10138	T10130	prep	with,Occurrence
R6721	T10139	T10140	amod	asymmetric,distribution
R6722	T10140	T10138	pobj	distribution,with
R6723	T10141	T10140	prep	of,distribution
R6724	T10142	T10141	pobj	adenine,of
R6725	T10143	T10142	cc	and,adenine
R6726	T10144	T10142	conj	thymine,adenine
R6727	T10145	T10140	punct	", ",distribution
R6728	T10146	T10140	prep	near,distribution
R6729	T10147	T10148	det	the,origins
R6730	T10148	T10146	pobj	origins,near
R6731	T10149	T10148	compound	replication,origins
R6732	T10150	T10131	punct	", ",lead
R6733	T10151	T10131	dobj	us,lead
R6734	T10152	T10153	aux	to,propose
R6735	T10153	T10131	xcomp	propose,lead
R6736	T10154	T10155	mark	that,play
R6737	T10155	T10153	ccomp	play,propose
R6738	T10156	T10155	nsubj	Mcm,play
R6739	T10157	T10155	aux	may,play
R6740	T10158	T10159	det	a,role
R6741	T10159	T10155	dobj	role,play
R6742	T10160	T10155	prep	in,play
R6743	T10161	T10160	pobj	selection,in
R6744	T10162	T10161	prep	of,selection
R6745	T10163	T10164	compound	initiation,sites
R6746	T10164	T10162	pobj	sites,of
R6747	T10165	T10164	prep	of,sites
R6748	T10166	T10167	amod	mammalian,DNA
R6749	T10167	T10168	compound	DNA,replication
R6750	T10168	T10165	pobj	replication,of
R6751	T10169	T10131	punct	", ",lead
R6752	T10170	T10131	cc	and,lead
R6753	T10171	T10131	conj	prompted,lead
R6754	T10172	T10171	dobj	us,prompted
R6755	T10173	T10174	aux	to,examine
R6756	T10174	T10171	xcomp	examine,prompted
R6757	T10175	T10176	det	the,ability
R6758	T10176	T10174	dobj	ability,examine
R6759	T10177	T10176	prep	of,ability
R6760	T10178	T10177	pobj	sequences,of
R6761	T10179	T10178	prep	from,sequences
R6762	T10180	T10181	amod	human,origins
R6763	T10181	T10179	pobj	origins,from
R6764	T10182	T10181	compound	replication,origins
R6765	T10183	T10184	aux	to,activate
R6766	T10184	T10176	acl	activate,ability
R6767	T10185	T10186	nmod	Mcm4,helicase
R6768	T10186	T10184	dobj	helicase,activate
R6769	T10187	T10185	punct	/,Mcm4
R6770	T10188	T10185	nummod	6,Mcm4
R6771	T10189	T10185	punct	/,Mcm4
R6772	T10190	T10185	nummod	7,Mcm4
R6773	T10191	T10131	punct	.,lead
R6774	T10193	T10194	preconj	Both,B2
R6775	T10194	T10197	nmod	B2,origins
R6776	T10195	T10194	nmod	lamin,B2
R6777	T10196	T10194	punct	-,B2
R6778	T10197	T10202	nsubj	origins,served
R6779	T10198	T10194	cc	and,B2
R6780	T10199	T10200	compound	c,myc
R6781	T10200	T10194	conj	myc,B2
R6782	T10201	T10200	punct	-,myc
R6783	T10203	T10202	prep	as,served
R6784	T10204	T10205	amod	efficient,activator
R6785	T10205	T10203	pobj	activator,as
R6786	T10206	T10205	prep	for,activator
R6787	T10207	T10208	compound	Mcm,helicase
R6788	T10208	T10206	pobj	helicase,for
R6789	T10209	T10210	advmod	in,vitro
R6790	T10210	T10202	advmod	vitro,served
R6791	T10211	T10202	punct	.,served
R6792	T10213	T10214	advcl	Consistent,reported
R6793	T10215	T10213	prep	with,Consistent
R6794	T10216	T10215	pobj	it,with
R6795	T10217	T10214	punct	", ",reported
R6796	T10218	T10219	npadvmod	site,specific
R6797	T10219	T10221	amod	specific,loading
R6798	T10220	T10219	punct	-,specific
R6799	T10221	T10214	nsubjpass	loading,reported
R6800	T10222	T10221	prep	of,loading
R6801	T10223	T10222	pobj	Mcm,of
R6802	T10224	T10221	prep	in,loading
R6803	T10225	T10226	det	the,zone
R6804	T10226	T10224	pobj	zone,in
R6805	T10227	T10228	compound	DNA,initiation
R6806	T10228	T10226	compound	initiation,zone
R6807	T10229	T10228	compound	replication,initiation
R6808	T10230	T10226	prep	of,zone
R6809	T10231	T10232	det	the,myc
R6810	T10232	T10230	pobj	myc,of
R6811	T10233	T10232	compound	c,myc
R6812	T10234	T10232	punct	-,myc
R6813	T10235	T10214	auxpass	was,reported
R6814	T10236	T10214	advmod	recently,reported
R6815	T10237	T10238	punct	(,33
R6816	T10238	T10214	parataxis	33,reported
R6817	T10239	T10238	punct	),33
R6818	T10240	T10214	punct	.,reported
R6819	T10242	T10243	nsubj	We,examined
R6820	T10244	T10243	aux	have,examined
R6821	T10245	T10243	prep	in,examined
R6822	T10246	T10245	pobj	detail,in
R6823	T10247	T10248	det	the,effect
R6824	T10248	T10243	dobj	effect,examined
R6825	T10249	T10248	prep	of,effect
R6826	T10250	T10251	compound	sequence,context
R6827	T10251	T10249	pobj	context,of
R6828	T10252	T10251	prep	of,context
R6829	T10253	T10254	det	the,DNA
R6830	T10254	T10252	pobj	DNA,of
R6831	T10255	T10256	amod	single,stranded
R6832	T10256	T10254	amod	stranded,DNA
R6833	T10257	T10256	punct	-,stranded
R6834	T10258	T10248	prep	on,effect
R6835	T10259	T10260	det	the,activity
R6836	T10260	T10258	pobj	activity,on
R6837	T10261	T10260	compound	helicase,activity
R6838	T10262	T10260	prep	of,activity
R6839	T10263	T10262	pobj	Mcm,of
R6840	T10264	T10243	punct	.,examined
R6841	T10266	T10267	det	The,results
R6842	T10267	T10268	nsubj	results,indicate
R6843	T10269	T10270	mark	that,is
R6844	T10270	T10268	ccomp	is,indicate
R6845	T10271	T10272	compound	thymine,content
R6846	T10272	T10270	nsubj	content,is
R6847	T10273	T10272	prep	of,content
R6848	T10274	T10275	nummod	50,%
R6849	T10275	T10273	pobj	%,of
R6850	T10276	T10270	acomp	sufficient,is
R6851	T10277	T10276	prep	for,sufficient
R6852	T10278	T10279	det	the,activity
R6853	T10279	T10277	pobj	activity,for
R6854	T10280	T10279	amod	maximum,activity
R6855	T10281	T10279	compound	helicase,activity
R6856	T10282	T10268	punct	.,indicate
R6857	T10284	T10285	det	The,efficiency
R6858	T10285	T10286	nsubj	efficiency,decreased
R6859	T10287	T10285	prep	of,efficiency
R6860	T10288	T10287	pobj	displacement,of
R6861	T10289	T10290	mark	as,dropped
R6862	T10290	T10286	advcl	dropped,decreased
R6863	T10291	T10292	det	the,content
R6864	T10292	T10290	nsubj	content,dropped
R6865	T10293	T10292	compound	thymine,content
R6866	T10294	T10292	prep	of,content
R6867	T10295	T10296	det	the,tail
R6868	T10296	T10294	pobj	tail,of
R6869	T10297	T10296	nummod	3,tail
R6870	T10298	T10297	punct	′,3
R6871	T10299	T10296	punct	-,tail
R6872	T10300	T10290	prep	to,dropped
R6873	T10301	T10302	nummod	33,%
R6874	T10302	T10300	pobj	%,to
R6875	T10303	T10304	punct	(,Figure
R6876	T10304	T10290	parataxis	Figure,dropped
R6877	T10305	T10304	nummod	6,Figure
R6878	T10306	T10304	punct	),Figure
R6879	T10307	T10286	punct	.,decreased
R6880	T10309	T10310	det	The,stretches
R6881	T10310	T10311	nsubjpass	stretches,required
R6882	T10312	T10310	prep	of,stretches
R6883	T10313	T10314	compound	thymine,residues
R6884	T10314	T10312	pobj	residues,of
R6885	T10315	T10311	aux	may,required
R6886	T10316	T10311	neg	not,required
R6887	T10317	T10311	auxpass	be,required
R6888	T10318	T10311	advmod	necessarily,required
R6889	T10319	T10311	punct	", ",required
R6890	T10320	T10321	mark	since,served
R6891	T10321	T10311	advcl	served,required
R6892	T10322	T10321	nsubj	repeats,served
R6893	T10323	T10322	prep	of,repeats
R6894	T10324	T10325	compound	TC,dinucleotides
R6895	T10325	T10323	pobj	dinucleotides,of
R6896	T10326	T10321	prep	as,served
R6897	T10327	T10328	det	a,activator
R6898	T10328	T10326	pobj	activator,as
R6899	T10329	T10328	amod	potent,activator
R6900	T10330	T10328	prep	for,activator
R6901	T10331	T10330	pobj	Mcm4,for
R6902	T10332	T10331	punct	/,Mcm4
R6903	T10333	T10331	nummod	6,Mcm4
R6904	T10334	T10331	punct	/,Mcm4
R6905	T10335	T10331	nummod	7,Mcm4
R6906	T10336	T10311	punct	.,required
R6907	T10338	T10339	nsubj	We,noticed
R6908	T10340	T10339	advmod	also,noticed
R6909	T10341	T10342	mark	that,is
R6910	T10342	T10339	ccomp	is,noticed
R6911	T10343	T10344	det	the,presence
R6912	T10344	T10342	nsubj	presence,is
R6913	T10345	T10344	prep	of,presence
R6914	T10346	T10347	det	a,structure
R6915	T10347	T10345	pobj	structure,of
R6916	T10348	T10347	amod	secondary,structure
R6917	T10349	T10347	prep	within,structure
R6918	T10350	T10351	det	the,DNA
R6919	T10351	T10349	pobj	DNA,within
R6920	T10352	T10353	amod	single,stranded
R6921	T10353	T10351	amod	stranded,DNA
R6922	T10354	T10353	punct	-,stranded
R6923	T10355	T10342	acomp	inhibitory,is
R6924	T10356	T10355	prep	for,inhibitory
R6925	T10357	T10358	compound	helicase,action
R6926	T10358	T10356	pobj	action,for
R6927	T10359	T10339	punct	.,noticed
R6928	T10361	T10362	compound	Nuclease,footprinting
R6929	T10362	T10363	compound	footprinting,assays
R6930	T10363	T10364	nsubj	assays,indicated
R6931	T10365	T10366	mark	that,interfered
R6932	T10366	T10364	ccomp	interfered,indicated
R6933	T10367	T10366	nsubjpass	binding,interfered
R6934	T10368	T10366	auxpass	was,interfered
R6935	T10369	T10366	agent	by,interfered
R6936	T10370	T10371	det	the,structure
R6937	T10371	T10369	pobj	structure,by
R6938	T10372	T10371	amod	secondary,structure
R6939	T10373	T10374	punct	(,shown
R6940	T10374	T10366	parataxis	shown,interfered
R6941	T10375	T10374	nsubj	data,shown
R6942	T10376	T10374	neg	not,shown
R6943	T10377	T10374	punct	),shown
R6944	T10378	T10364	punct	.,indicated
R6945	T10380	T10381	advmod	Thus,concluded
R6946	T10382	T10381	punct	", ",concluded
R6947	T10383	T10381	nsubj	we,concluded
R6948	T10384	T10381	aux	have,concluded
R6949	T10385	T10386	mark	that,activated
R6950	T10386	T10381	ccomp	activated,concluded
R6951	T10387	T10388	nmod	Mcm4,helicase
R6952	T10388	T10386	nsubjpass	helicase,activated
R6953	T10389	T10387	punct	/,Mcm4
R6954	T10390	T10387	nummod	6,Mcm4
R6955	T10391	T10387	punct	/,Mcm4
R6956	T10392	T10387	nummod	7,Mcm4
R6957	T10393	T10386	auxpass	is,activated
R6958	T10394	T10395	advmod	most,efficiently
R6959	T10395	T10386	advmod	efficiently,activated
R6960	T10396	T10386	agent	by,activated
R6961	T10397	T10398	amod	non-structured,DNA
R6962	T10398	T10396	pobj	DNA,by
R6963	T10399	T10400	amod	single,stranded
R6964	T10400	T10398	amod	stranded,DNA
R6965	T10401	T10400	punct	-,stranded
R6966	T10402	T10398	prep	with,DNA
R6967	T10403	T10404	compound	thymine,content
R6968	T10404	T10402	pobj	content,with
R6969	T10405	T10404	prep	of,content
R6970	T10406	T10407	nummod	50,%
R6971	T10407	T10405	pobj	%,of
R6972	T10408	T10409	cc	or,more
R6973	T10409	T10407	nummod	more,%
R6974	T10410	T10386	punct	", ",activated
R6975	T10411	T10412	mark	although,observed
R6976	T10412	T10386	advcl	observed,activated
R6977	T10413	T10414	amod	significant,stimulation
R6978	T10414	T10412	nsubjpass	stimulation,observed
R6979	T10415	T10412	auxpass	is,observed
R6980	T10416	T10412	advmod	also,observed
R6981	T10417	T10412	agent	by,observed
R6982	T10418	T10417	pobj	DNA,by
R6983	T10419	T10418	prep	with,DNA
R6984	T10420	T10421	amod	less,content
R6985	T10421	T10419	pobj	content,with
R6986	T10422	T10421	compound	thymine,content
R6987	T10423	T10424	punct	(,Figure
R6988	T10424	T10418	parataxis	Figure,DNA
R6989	T10425	T10424	nummod	7,Figure
R6990	T10426	T10424	punct	),Figure
R6991	T10427	T10412	punct	", ",observed
R6992	T10428	T10412	advcl	suggesting,observed
R6993	T10429	T10430	mark	that,is
R6994	T10430	T10428	advcl	is,suggesting
R6995	T10431	T10432	det	the,specificity
R6996	T10432	T10430	nsubj	specificity,is
R6997	T10433	T10432	compound	sequence,specificity
R6998	T10434	T10432	prep	for,specificity
R6999	T10435	T10436	compound	Mcm,helicase
R7000	T10436	T10437	compound	helicase,activation
R7001	T10437	T10434	pobj	activation,for
R7002	T10438	T10439	advmod	rather,relaxed
R7003	T10439	T10430	acomp	relaxed,is
R7004	T10440	T10430	cc	and,is
R7005	T10441	T10442	mark	that,depend
R7006	T10442	T10430	conj	depend,is
R7007	T10443	T10444	det	the,extent
R7008	T10444	T10442	nsubj	extent,depend
R7009	T10445	T10444	prep	of,extent
R7010	T10446	T10447	det	the,activation
R7011	T10447	T10445	pobj	activation,of
R7012	T10448	T10442	aux	may,depend
R7013	T10449	T10442	prep	on,depend
R7014	T10450	T10451	det	the,context
R7015	T10451	T10449	pobj	context,on
R7016	T10452	T10451	compound	sequence,context
R7017	T10453	T10381	punct	.,concluded
R7018	T10455	T10456	nsubj	This,be
R7019	T10457	T10456	aux	would,be
R7020	T10458	T10456	acomp	reasonable,be
R7021	T10459	T10456	prep	given,be
R7022	T10460	T10461	det	the,flexibility
R7023	T10461	T10459	pobj	flexibility,given
R7024	T10462	T10461	cc	and,flexibility
R7025	T10463	T10464	amod	differential,regulation
R7026	T10464	T10461	conj	regulation,flexibility
R7027	T10465	T10461	prep	of,flexibility
R7028	T10466	T10467	compound	site,selection
R7029	T10467	T10465	pobj	selection,of
R7030	T10468	T10461	prep	for,flexibility
R7031	T10469	T10468	pobj	initiation,for
R7032	T10470	T10469	prep	of,initiation
R7033	T10471	T10472	compound	DNA,replication
R7034	T10472	T10470	pobj	replication,of
R7035	T10473	T10469	prep	during,initiation
R7036	T10474	T10473	pobj	development,during
R7037	T10475	T10468	cc	or,for
R7038	T10476	T10468	conj	in,for
R7039	T10477	T10478	amod	various,types
R7040	T10478	T10476	pobj	types,in
R7041	T10479	T10478	compound	cell,types
R7042	T10480	T10461	punct	", ",flexibility
R7043	T10481	T10482	advmod	as,as
R7044	T10482	T10461	cc	as,flexibility
R7045	T10483	T10482	advmod	well,as
R7046	T10484	T10485	det	the,variability
R7047	T10485	T10461	conj	variability,flexibility
R7048	T10486	T10485	prep	in,variability
R7049	T10487	T10488	compound	initiation,potential
R7050	T10488	T10486	pobj	potential,in
R7051	T10489	T10488	prep	of,potential
R7052	T10490	T10491	det	each,origin
R7053	T10491	T10489	pobj	origin,of
R7054	T10492	T10491	compound	replication,origin
R7055	T10493	T10491	prep	on,origin
R7056	T10494	T10495	det	the,genome
R7057	T10495	T10493	pobj	genome,on
R7058	T10496	T10497	advmod	even,within
R7059	T10497	T10485	prep	within,variability
R7060	T10498	T10499	det	the,type
R7061	T10499	T10497	pobj	type,within
R7062	T10500	T10499	amod	single,type
R7063	T10501	T10499	compound	cell,type
R7064	T10502	T10456	punct	.,be
R7067	T10855	T10856	nmod	Mcm4,helicase
R7068	T10857	T10855	punct	/,Mcm4
R7069	T10858	T10855	nummod	6,Mcm4
R7070	T10859	T10855	punct	/,Mcm4
R7071	T10860	T10855	nummod	7,Mcm4
R7072	T10861	T10856	prep	during,helicase
R7073	T10862	T10863	amod	processive,unwinding
R7074	T10863	T10861	pobj	unwinding,during
R7075	T10864	T10856	prep	at,helicase
R7076	T10865	T10866	det	the,fork
R7077	T10866	T10864	pobj	fork,at
R7078	T10868	T10869	det	The,requirement
R7079	T10869	T10871	nsubj	requirement,prompted
R7080	T10870	T10869	amod	specific,requirement
R7081	T10872	T10869	prep	of,requirement
R7082	T10873	T10874	amod	single,stranded
R7083	T10874	T10876	amod	stranded,residues
R7084	T10875	T10874	punct	-,stranded
R7085	T10876	T10872	pobj	residues,of
R7086	T10877	T10876	compound	thymine,residues
R7087	T10878	T10869	prep	for,requirement
R7088	T10879	T10878	pobj	activation,for
R7089	T10880	T10879	prep	of,activation
R7090	T10881	T10882	compound	Mcm,helicase
R7091	T10882	T10880	pobj	helicase,of
R7092	T10883	T10871	dobj	us,prompted
R7093	T10884	T10885	aux	to,examine
R7094	T10885	T10871	xcomp	examine,prompted
R7095	T10886	T10887	mark	whether,required
R7096	T10887	T10885	ccomp	required,examine
R7097	T10888	T10887	nsubjpass	they,required
R7098	T10889	T10887	auxpass	are,required
R7099	T10890	T10887	advmod	also,required
R7100	T10891	T10887	prep	for,required
R7101	T10892	T10893	amod	processive,unwinding
R7102	T10893	T10891	pobj	unwinding,for
R7103	T10894	T10893	prep	of,unwinding
R7104	T10895	T10896	compound	duplex,DNA
R7105	T10896	T10894	pobj	DNA,of
R7106	T10897	T10871	punct	.,prompted
R7107	T10899	T10900	poss	Our,results
R7108	T10900	T10901	nsubj	results,indicated
R7109	T10902	T10903	mark	that,inhibited
R7110	T10903	T10901	ccomp	inhibited,indicated
R7111	T10904	T10903	nsubj	increase,inhibited
R7112	T10905	T10904	prep	of,increase
R7113	T10906	T10907	compound	GC,pair
R7114	T10907	T10905	pobj	pair,of
R7115	T10908	T10904	prep	in,increase
R7116	T10909	T10910	det	the,segment
R7117	T10910	T10908	pobj	segment,in
R7118	T10911	T10910	compound	duplex,segment
R7119	T10912	T10903	advmod	significantly,inhibited
R7120	T10913	T10914	det	the,activity
R7121	T10914	T10903	dobj	activity,inhibited
R7122	T10915	T10916	compound	Mcm,helicase
R7123	T10916	T10914	compound	helicase,activity
R7124	T10917	T10901	punct	.,indicated
R7125	T10919	T10920	compound	Duplex,DNA
R7126	T10920	T10921	nsubjpass	DNA,displaced
R7127	T10922	T10920	acl	composed,DNA
R7128	T10923	T10924	advmod	only,of
R7129	T10924	T10922	prep	of,composed
R7130	T10925	T10926	compound	GC,pairs
R7131	T10926	T10924	pobj	pairs,of
R7132	T10927	T10928	punct	(,repeats
R7133	T10928	T10926	parataxis	repeats,pairs
R7134	T10929	T10928	nummod	10,repeats
R7135	T10930	T10928	prep	of,repeats
R7136	T10931	T10930	pobj	CGG,of
R7137	T10932	T10928	punct	),repeats
R7138	T10933	T10926	prep	on,pairs
R7139	T10934	T10935	det	a,fork
R7140	T10935	T10933	pobj	fork,on
R7141	T10936	T10935	compound	Y,fork
R7142	T10937	T10935	punct	-,fork
R7143	T10938	T10921	auxpass	was,displaced
R7144	T10939	T10921	neg	not,displaced
R7145	T10940	T10941	advmod	at,all
R7146	T10941	T10921	advmod	all,displaced
R7147	T10942	T10921	punct	", ",displaced
R7148	T10943	T10944	mark	while,displaced
R7149	T10944	T10921	advcl	displaced,displaced
R7150	T10945	T10946	det	the,template
R7151	T10946	T10944	nsubjpass	template,displaced
R7152	T10947	T10946	amod	same,template
R7153	T10948	T10944	auxpass	was,displaced
R7154	T10949	T10944	advmod	readily,displaced
R7155	T10950	T10944	agent	by,displaced
R7156	T10951	T10952	compound	SV40,antigen
R7157	T10952	T10950	pobj	antigen,by
R7158	T10953	T10952	compound	T,antigen
R7159	T10954	T10952	punct	-,antigen
R7160	T10955	T10956	punct	(,8B
R7161	T10956	T10944	parataxis	8B,displaced
R7162	T10957	T10956	compound	Figure,8B
R7163	T10958	T10956	punct	),8B
R7164	T10959	T10921	punct	.,displaced
R7165	T10961	T10962	compound	Mcm,helicase
R7166	T10962	T10963	nsubjpass	helicase,inhibited
R7167	T10964	T10963	auxpass	was,inhibited
R7168	T10965	T10963	agent	by,inhibited
R7169	T10966	T10967	det	the,presence
R7170	T10967	T10965	pobj	presence,by
R7171	T10968	T10967	prep	of,presence
R7172	T10969	T10970	npadvmod	GC,rich
R7173	T10970	T10972	amod	rich,segment
R7174	T10971	T10970	punct	-,rich
R7175	T10972	T10968	pobj	segment,of
R7176	T10973	T10972	compound	duplex,segment
R7177	T10974	T10975	advmod	also,on
R7178	T10975	T10967	prep	on,presence
R7179	T10976	T10977	det	a,substrate
R7180	T10977	T10975	pobj	substrate,on
R7181	T10978	T10977	amod	circular,substrate
R7182	T10979	T10980	amod	single,stranded
R7183	T10980	T10977	amod	stranded,substrate
R7184	T10981	T10980	punct	-,stranded
R7185	T10982	T10983	amod	partial,heteroduplex
R7186	T10983	T10977	compound	heteroduplex,substrate
R7187	T10984	T10963	punct	.,inhibited
R7188	T10986	T10987	advmod	However,was
R7189	T10988	T10987	punct	", ",was
R7190	T10989	T10987	prep	on,was
R7191	T10990	T10991	det	this,substrate
R7192	T10991	T10989	pobj	substrate,on
R7193	T10992	T10987	punct	", ",was
R7194	T10993	T10987	nsubj	Mcm4,was
R7195	T10994	T10993	punct	/,Mcm4
R7196	T10995	T10993	nummod	6,Mcm4
R7197	T10996	T10993	punct	/,Mcm4
R7198	T10997	T10993	nummod	7,Mcm4
R7199	T10998	T10987	acomp	able,was
R7200	T10999	T11000	aux	to,displace
R7201	T11000	T10998	xcomp	displace,able
R7202	T11001	T11000	dobj	DNA,displace
R7203	T11002	T11000	prep	past,displace
R7204	T11003	T11004	det	the,region
R7205	T11004	T11002	pobj	region,past
R7206	T11005	T11006	npadvmod	GC,rich
R7207	T11006	T11004	amod	rich,region
R7208	T11007	T11006	punct	-,rich
R7209	T11008	T11000	punct	", ",displace
R7210	T11009	T11010	dep	albeit,to
R7211	T11010	T11000	prep	to,displace
R7212	T11011	T11012	det	a,extent
R7213	T11012	T11010	pobj	extent,to
R7214	T11013	T11012	amod	limited,extent
R7215	T11014	T11000	punct	", ",displace
R7216	T11015	T11016	advmod	when,added
R7217	T11016	T11000	advcl	added,displace
R7218	T11017	T11016	nsubjpass	it,added
R7219	T11018	T11016	auxpass	was,added
R7220	T11019	T11016	prep	at,added
R7221	T11020	T11021	det	a,concentration
R7222	T11021	T11019	pobj	concentration,at
R7223	T11022	T11021	amod	high,concentration
R7224	T11023	T10987	punct	.,was
R7225	T11025	T11026	prep	On,loaded
R7226	T11027	T11028	det	the,template
R7227	T11028	T11025	pobj	template,On
R7228	T11029	T11030	amod	partial,heteroduplex
R7229	T11030	T11028	compound	heteroduplex,template
R7230	T11031	T11026	punct	", ",loaded
R7231	T11032	T11026	nsubjpass	Mcm,loaded
R7232	T11033	T11026	auxpass	is,loaded
R7233	T11034	T11026	prep	onto,loaded
R7234	T11035	T11036	det	the,DNA
R7235	T11036	T11034	pobj	DNA,onto
R7236	T11037	T11036	amod	circular,DNA
R7237	T11038	T11039	amod	single,stranded
R7238	T11039	T11036	amod	stranded,DNA
R7239	T11040	T11039	punct	-,stranded
R7240	T11041	T11036	prep	of,DNA
R7241	T11042	T11043	nummod	6.4,kb
R7242	T11043	T11041	pobj	kb,of
R7243	T11044	T11026	punct	", ",loaded
R7244	T11045	T11046	mark	while,loaded
R7245	T11046	T11026	advcl	loaded,loaded
R7246	T11047	T11046	nsubjpass	it,loaded
R7247	T11048	T11046	auxpass	is,loaded
R7248	T11049	T11046	prep	onto,loaded
R7249	T11050	T11051	det	the,DNA
R7250	T11051	T11049	pobj	DNA,onto
R7251	T11052	T11053	nummod	50,nt
R7252	T11053	T11054	npadvmod	nt,long
R7253	T11054	T11051	amod	long,DNA
R7254	T11055	T11056	nummod	3,tail
R7255	T11056	T11051	compound	tail,DNA
R7256	T11057	T11055	punct	′,3
R7257	T11058	T11056	punct	-,tail
R7258	T11059	T11051	prep	on,DNA
R7259	T11060	T11061	det	the,fork
R7260	T11061	T11059	pobj	fork,on
R7261	T11062	T11061	compound	Y,fork
R7262	T11063	T11061	punct	-,fork
R7263	T11064	T11026	punct	.,loaded
R7264	T11066	T11067	advmod	Thus,reflect
R7265	T11068	T11067	punct	", ",reflect
R7266	T11069	T11070	det	the,difference
R7267	T11070	T11067	nsubj	difference,reflect
R7268	T11071	T11070	prep	of,difference
R7269	T11072	T11073	compound	helicase,actions
R7270	T11073	T11071	pobj	actions,of
R7271	T11074	T11067	aux	may,reflect
R7272	T11075	T11076	det	the,efficiency
R7273	T11076	T11067	dobj	efficiency,reflect
R7274	T11077	T11076	prep	of,efficiency
R7275	T11078	T11079	compound	Mcm,loading
R7276	T11079	T11077	pobj	loading,of
R7277	T11080	T11067	punct	.,reflect
R7278	T11082	T11083	advmod	Alternatively,engage
R7279	T11084	T11083	punct	", ",engage
R7280	T11085	T11086	det	the,presence
R7281	T11086	T11083	nsubj	presence,engage
R7282	T11087	T11086	prep	of,presence
R7283	T11088	T11087	punct	‘,of
R7284	T11089	T11090	amod	random,sequence
R7285	T11090	T11087	pobj	sequence,of
R7286	T11091	T11090	punct	’,sequence
R7287	T11092	T11086	prep	at,presence
R7288	T11093	T11094	det	the,segment
R7289	T11094	T11092	pobj	segment,at
R7290	T11095	T11096	advmod	initially,unwound
R7291	T11096	T11094	amod	unwound,segment
R7292	T11097	T11094	compound	duplex,segment
R7293	T11098	T11094	prep	in,segment
R7294	T11099	T11100	det	the,template
R7295	T11100	T11098	pobj	template,in
R7296	T11101	T11100	amod	former,template
R7297	T11102	T11083	aux	may,engage
R7298	T11103	T11104	det	the,helicase
R7299	T11104	T11083	dobj	helicase,engage
R7300	T11105	T11104	compound	Mcm,helicase
R7301	T11106	T11083	prep	in,engage
R7302	T11107	T11108	det	a,conformation
R7303	T11108	T11106	pobj	conformation,in
R7304	T11109	T11110	advmod	more,active
R7305	T11110	T11108	amod	active,conformation
R7306	T11111	T11112	dep	which,displace
R7307	T11112	T11108	relcl	displace,conformation
R7308	T11113	T11112	aux	can,displace
R7309	T11114	T11115	det	the,segment
R7310	T11115	T11112	dobj	segment,displace
R7311	T11116	T11117	npadvmod	GC,rich
R7312	T11117	T11115	amod	rich,segment
R7313	T11118	T11117	punct	-,rich
R7314	T11119	T11115	compound	duplex,segment
R7315	T11120	T11083	punct	.,engage
R7316	T11122	T11123	prep	On,restored
R7317	T11124	T11125	compound	Y,fork
R7318	T11125	T11127	compound	fork,substrates
R7319	T11126	T11125	punct	-,fork
R7320	T11127	T11122	pobj	substrates,On
R7321	T11128	T11123	punct	", ",restored
R7322	T11129	T11123	nsubj	increase,restored
R7323	T11130	T11129	prep	of,increase
R7324	T11131	T11132	compound	AT,pairs
R7325	T11132	T11130	pobj	pairs,of
R7326	T11133	T11132	compound	base,pairs
R7327	T11134	T11129	prep	in,increase
R7328	T11135	T11136	det	the,duplex
R7329	T11136	T11134	pobj	duplex,in
R7330	T11137	T11138	punct	(,repeats
R7331	T11138	T11136	parataxis	repeats,duplex
R7332	T11139	T11138	nummod	10,repeats
R7333	T11140	T11138	prep	of,repeats
R7334	T11141	T11140	pobj	CTT,of
R7335	T11142	T11138	punct	),repeats
R7336	T11143	T11144	det	the,unwinding
R7337	T11144	T11123	dobj	unwinding,restored
R7338	T11145	T11123	punct	.,restored
R7339	T11147	T11148	det	These,results
R7340	T11148	T11149	nsubj	results,indicate
R7341	T11150	T11151	preconj	either,required
R7342	T11151	T11149	advcl	required,indicate
R7343	T11152	T11151	mark	that,required
R7344	T11153	T11154	compound	thymine,residues
R7345	T11154	T11151	nsubjpass	residues,required
R7346	T11155	T11151	auxpass	are,required
R7347	T11156	T11151	prep	on,required
R7348	T11157	T11158	det	the,DNA
R7349	T11158	T11156	pobj	DNA,on
R7350	T11159	T11158	compound	duplex,DNA
R7351	T11160	T11151	prep	for,required
R7352	T11161	T11162	amod	continuous,unwinding
R7353	T11162	T11160	pobj	unwinding,for
R7354	T11163	T11151	punct	", ",required
R7355	T11164	T11151	cc	or,required
R7356	T11165	T11166	mark	that,is
R7357	T11166	T11151	conj	is,required
R7358	T11167	T11166	nsubj	Mcm4,is
R7359	T11168	T11167	punct	/,Mcm4
R7360	T11169	T11167	nummod	6,Mcm4
R7361	T11170	T11167	punct	/,Mcm4
R7362	T11171	T11167	nummod	7,Mcm4
R7363	T11172	T11166	advmod	simply,is
R7364	T11173	T11166	neg	not,is
R7365	T11174	T11166	acomp	efficient,is
R7366	T11175	T11174	advmod	enough,efficient
R7367	T11176	T11177	aux	to,unwind
R7368	T11177	T11174	xcomp	unwind,efficient
R7369	T11178	T11179	det	the,pairs
R7370	T11179	T11177	dobj	pairs,unwind
R7371	T11180	T11179	amod	stable,pairs
R7372	T11181	T11179	compound	GC,pairs
R7373	T11182	T11149	punct	.,indicate
R7374	T11184	T11185	nsubj	Replacement,resulted
R7375	T11186	T11184	prep	of,Replacement
R7376	T11187	T11188	det	the,guanosine
R7377	T11188	T11186	pobj	guanosine,of
R7378	T11189	T11188	amod	central,guanosine
R7379	T11190	T11184	prep	with,Replacement
R7380	T11191	T11190	pobj	inosine,with
R7381	T11192	T11184	prep	in,Replacement
R7382	T11193	T11194	det	the,DNA
R7383	T11194	T11192	pobj	DNA,in
R7384	T11195	T11196	compound	CGG,repeat
R7385	T11196	T11194	compound	repeat,DNA
R7386	T11197	T11194	compound	duplex,DNA
R7387	T11198	T11185	prep	in,resulted
R7388	T11199	T11198	pobj	displacement,in
R7389	T11200	T11201	punct	(,8E
R7390	T11201	T11185	parataxis	8E,resulted
R7391	T11202	T11201	compound	Figure,8E
R7392	T11203	T11201	punct	),8E
R7393	T11204	T11205	punct	(,31
R7394	T11205	T11185	parataxis	31,resulted
R7395	T11206	T11205	punct	),31
R7396	T11207	T11185	punct	", ",resulted
R7397	T11208	T11185	advcl	suggesting,resulted
R7398	T11209	T11210	mark	that,be
R7399	T11210	T11208	advcl	be,suggesting
R7400	T11211	T11212	det	the,presence
R7401	T11212	T11210	nsubj	presence,be
R7402	T11213	T11212	amod	continuous,presence
R7403	T11214	T11212	prep	of,presence
R7404	T11215	T11216	compound	AT,pair
R7405	T11216	T11214	pobj	pair,of
R7406	T11217	T11216	compound	base,pair
R7407	T11218	T11210	aux	may,be
R7408	T11219	T11210	neg	not,be
R7409	T11220	T11210	acomp	essential,be
R7410	T11221	T11220	prep	for,essential
R7411	T11222	T11223	det	the,function
R7412	T11223	T11221	pobj	function,for
R7413	T11224	T11225	compound	Mcm,helicase
R7414	T11225	T11223	compound	helicase,function
R7415	T11226	T11210	cc	and,be
R7416	T11227	T11228	mark	that,is
R7417	T11228	T11210	conj	is,be
R7418	T11229	T11230	det	the,thermostability
R7419	T11230	T11228	nsubj	thermostability,is
R7420	T11231	T11230	prep	of,thermostability
R7421	T11232	T11233	compound	GC,pairs
R7422	T11233	T11231	pobj	pairs,of
R7423	T11234	T11233	compound	base,pairs
R7424	T11235	T11228	acomp	inhibitory,is
R7425	T11236	T11235	prep	for,inhibitory
R7426	T11237	T11238	compound	helicase,action
R7427	T11238	T11236	pobj	action,for
R7428	T11239	T11238	prep	of,action
R7429	T11240	T11239	pobj	Mcm4,of
R7430	T11241	T11240	punct	/,Mcm4
R7431	T11242	T11240	nummod	6,Mcm4
R7432	T11243	T11240	punct	/,Mcm4
R7433	T11244	T11240	nummod	7,Mcm4
R7434	T11245	T11185	punct	.,resulted
R7435	T11247	T11248	det	The,results
R7436	T11248	T11249	nsubj	results,reveal
R7437	T11250	T11248	acl	described,results
R7438	T11251	T11250	prep	in,described
R7439	T11252	T11253	det	this,manuscript
R7440	T11253	T11251	pobj	manuscript,in
R7441	T11254	T11255	advmod	potentially,important
R7442	T11255	T11256	amod	important,features
R7443	T11256	T11249	dobj	features,reveal
R7444	T11257	T11256	prep	of,features
R7445	T11258	T11259	amod	mammalian,helicase
R7446	T11259	T11257	pobj	helicase,of
R7447	T11260	T11259	nmod	Mcm4,helicase
R7448	T11261	T11260	punct	/,Mcm4
R7449	T11262	T11260	nummod	6,Mcm4
R7450	T11263	T11260	punct	/,Mcm4
R7451	T11264	T11260	nummod	7,Mcm4
R7452	T11265	T11259	punct	", ",helicase
R7453	T11266	T11267	dep	which,is
R7454	T11267	T11259	relcl	is,helicase
R7455	T11268	T11267	acomp	likely,is
R7456	T11269	T11270	aux	to,be
R7457	T11270	T11268	xcomp	be,likely
R7458	T11271	T11272	det	a,component
R7459	T11272	T11270	attr	component,be
R7460	T11273	T11272	amod	key,component
R7461	T11274	T11272	prep	of,component
R7462	T11275	T11276	det	the,helicase
R7463	T11276	T11274	pobj	helicase,of
R7464	T11277	T11276	amod	eukaryotic,helicase
R7465	T11278	T11276	amod	replicative,helicase
R7466	T11279	T11249	punct	.,reveal
R7467	T11281	T11282	amod	Prior,to
R7468	T11282	T11283	prep	to,adopt
R7469	T11284	T11282	pobj	initiation,to
R7470	T11285	T11284	prep	of,initiation
R7471	T11286	T11287	compound	DNA,replication
R7472	T11287	T11285	pobj	replication,of
R7473	T11288	T11283	punct	", ",adopt
R7474	T11289	T11290	compound	Mcm,helicase
R7475	T11290	T11283	nsubj	helicase,adopt
R7476	T11291	T11283	aux	may,adopt
R7477	T11292	T11293	det	a,complex
R7478	T11293	T11283	dobj	complex,adopt
R7479	T11294	T11295	amod	double,hexameric
R7480	T11295	T11293	amod	hexameric,complex
R7481	T11296	T11283	prep	at,adopt
R7482	T11297	T11298	det	the,region
R7483	T11298	T11296	pobj	region,at
R7484	T11299	T11300	advmod	partially,melted
R7485	T11300	T11298	amod	melted,region
R7486	T11301	T11298	compound	origin,region
R7487	T11302	T11283	punct	", ",adopt
R7488	T11303	T11283	cc	and,adopt
R7489	T11304	T11305	aux	may,catalyze
R7490	T11305	T11283	conj	catalyze,adopt
R7491	T11306	T11307	amod	concurrent,unwinding
R7492	T11307	T11305	dobj	unwinding,catalyze
R7493	T11308	T11307	prep	of,unwinding
R7494	T11309	T11310	compound	duplex,DNA
R7495	T11310	T11308	pobj	DNA,of
R7496	T11311	T11305	prep	into,catalyze
R7497	T11312	T11313	det	both,directions
R7498	T11313	T11311	pobj	directions,into
R7499	T11314	T11283	punct	", ",adopt
R7500	T11315	T11316	mark	while,associated
R7501	T11316	T11283	advcl	associated,adopt
R7502	T11317	T11316	advmod	stably,associated
R7503	T11318	T11316	prep	with,associated
R7504	T11319	T11320	det	the,origins
R7505	T11320	T11318	pobj	origins,with
R7506	T11321	T11320	prep	of,origins
R7507	T11322	T11323	compound	DNA,replication
R7508	T11323	T11321	pobj	replication,of
R7509	T11324	T11283	punct	.,adopt
R7510	T11326	T11327	nsubj	This,is
R7511	T11328	T11327	acomp	similar,is
R7512	T11329	T11328	prep	to,similar
R7513	T11330	T11331	det	the,model
R7514	T11331	T11329	pobj	model,to
R7515	T11332	T11333	compound	T,antigen
R7516	T11333	T11331	compound	antigen,model
R7517	T11334	T11333	punct	-,antigen
R7518	T11335	T11336	advmod	originally,proposed
R7519	T11336	T11331	acl	proposed,model
R7520	T11337	T11336	agent	by,proposed
R7521	T11338	T11337	pobj	Smelkova,by
R7522	T11339	T11338	cc	and,Smelkova
R7523	T11340	T11338	conj	Borowiec,Smelkova
R7524	T11341	T11342	punct	(,21
R7525	T11342	T11340	parataxis	21,Borowiec
R7526	T11343	T11342	punct	),21
R7527	T11344	T11327	punct	.,is
R7528	T11346	T11347	nsubj	We,propose
R7529	T11348	T11349	mark	that,mobilized
R7530	T11349	T11347	ccomp	mobilized,propose
R7531	T11350	T11349	punct	", ",mobilized
R7532	T11351	T11352	advmod	only,activated
R7533	T11352	T11349	advcl	activated,mobilized
R7534	T11353	T11352	advmod	when,activated
R7535	T11354	T11355	det	both,hexamers
R7536	T11355	T11352	nsubjpass	hexamers,activated
R7537	T11356	T11352	auxpass	are,activated
R7538	T11357	T11352	agent	by,activated
R7539	T11358	T11359	det	the,sequences
R7540	T11359	T11357	pobj	sequences,by
R7541	T11360	T11359	amod	interacting,sequences
R7542	T11361	T11362	npadvmod	thymine,rich
R7543	T11362	T11359	amod	rich,sequences
R7544	T11363	T11362	punct	-,rich
R7545	T11364	T11359	relcl	present,sequences
R7546	T11365	T11364	prep	within,present
R7547	T11366	T11367	det	the,region
R7548	T11367	T11365	pobj	region,within
R7549	T11368	T11367	amod	melted,region
R7550	T11369	T11349	punct	", ",mobilized
R7551	T11370	T11371	det	the,helicase
R7552	T11371	T11349	nsubjpass	helicase,mobilized
R7553	T11372	T11371	compound	Mcm,helicase
R7554	T11373	T11349	auxpass	is,mobilized
R7555	T11374	T11349	cc	and,mobilized
R7556	T11375	T11376	nsubj	initiation,takes
R7557	T11376	T11349	conj	takes,mobilized
R7558	T11377	T11376	dobj	place,takes
R7559	T11378	T11347	punct	.,propose
R7560	T11380	T11381	det	A,question
R7561	T11381	T11383	nsubj	question,is
R7562	T11382	T11381	amod	crucial,question
R7563	T11384	T11385	advmod	how,generated
R7564	T11385	T11383	ccomp	generated,is
R7565	T11386	T11387	det	these,complexes
R7566	T11387	T11385	nsubjpass	complexes,generated
R7567	T11388	T11389	advmod	double,hexameric
R7568	T11389	T11387	amod	hexameric,complexes
R7569	T11390	T11387	compound	helicase,complexes
R7570	T11391	T11385	auxpass	are,generated
R7571	T11392	T11385	cc	and,generated
R7572	T11393	T11385	conj	turn,generated
R7573	T11394	T11393	prep	into,turn
R7574	T11395	T11396	amod	active,helicases
R7575	T11396	T11394	pobj	helicases,into
R7576	T11397	T11393	prep	at,turn
R7577	T11398	T11399	det	the,origins
R7578	T11399	T11397	pobj	origins,at
R7579	T11400	T11383	punct	.,is
R7580	T11402	T11403	det	This,process
R7581	T11403	T11404	nsubj	process,involve
R7582	T11405	T11404	aux	would,involve
R7583	T11406	T11404	dobj	melting,involve
R7584	T11407	T11406	prep	of,melting
R7585	T11408	T11409	compound	duplex,DNA
R7586	T11409	T11407	pobj	DNA,of
R7587	T11410	T11406	punct	", ",melting
R7588	T11411	T11412	dep	which,facilitated
R7589	T11412	T11406	relcl	facilitated,melting
R7590	T11413	T11412	aux	may,facilitated
R7591	T11414	T11412	auxpass	be,facilitated
R7592	T11415	T11412	prep	by,facilitated
R7593	T11416	T11415	pobj	binding,by
R7594	T11417	T11416	prep	of,binding
R7595	T11418	T11419	compound	preRC,components
R7596	T11419	T11417	pobj	components,of
R7597	T11420	T11416	prep	in,binding
R7598	T11421	T11422	det	the,context
R7599	T11422	T11420	pobj	context,in
R7600	T11423	T11422	prep	of,context
R7601	T11424	T11425	amod	proper,structures
R7602	T11425	T11423	pobj	structures,of
R7603	T11426	T11425	compound	chromatin,structures
R7604	T11427	T11415	punct	", ",by
R7605	T11428	T11415	cc	or,by
R7606	T11429	T11415	conj	by,by
R7607	T11430	T11431	amod	other,factors
R7608	T11431	T11429	pobj	factors,by
R7609	T11432	T11431	amod	unwinding,factors
R7610	T11433	T11431	prep	including,factors
R7611	T11434	T11435	det	a,topoisomerase
R7612	T11435	T11433	pobj	topoisomerase,including
R7613	T11436	T11435	cc	or,topoisomerase
R7614	T11437	T11438	det	a,helicase
R7615	T11438	T11435	conj	helicase,topoisomerase
R7616	T11439	T11440	advmod	newly,identified
R7617	T11440	T11438	amod	identified,helicase
R7618	T11441	T11438	compound	Mcm8,helicase
R7619	T11442	T11443	punct	(,34
R7620	T11443	T11404	parataxis	34,involve
R7621	T11444	T11445	punct	–,36
R7622	T11445	T11443	prep	36,34
R7623	T11446	T11443	punct	),34
R7624	T11447	T11404	punct	.,involve
R7625	T11449	T11450	nsubj	It,require
R7626	T11451	T11450	aux	may,require
R7627	T11452	T11450	advmod	also,require
R7628	T11453	T11450	dobj	association,require
R7629	T11454	T11453	prep	of,association
R7630	T11455	T11456	npadvmod	fork,assisting
R7631	T11456	T11458	amod	assisting,proteins
R7632	T11457	T11456	punct	-,assisting
R7633	T11458	T11454	pobj	proteins,of
R7634	T11459	T11460	amod	such,as
R7635	T11460	T11458	prep	as,proteins
R7636	T11461	T11460	pobj	Cdc45,as
R7637	T11462	T11463	punct	(,38
R7638	T11463	T11461	parataxis	38,Cdc45
R7639	T11464	T11463	nummod	37,38
R7640	T11465	T11463	punct	",",38
R7641	T11466	T11463	punct	),38
R7642	T11467	T11468	advmod	as,as
R7643	T11468	T11458	cc	as,proteins
R7644	T11469	T11468	advmod	well,as
R7645	T11470	T11471	compound	phosphorylation,events
R7646	T11471	T11458	conj	events,proteins
R7647	T11472	T11471	prep	by,events
R7648	T11473	T11474	nmod	Cdk,kinases
R7649	T11474	T11472	pobj	kinases,by
R7650	T11475	T11473	cc	and,Cdk
R7651	T11476	T11473	conj	Cdc7,Cdk
R7652	T11477	T11478	punct	(,40
R7653	T11478	T11474	parataxis	40,kinases
R7654	T11479	T11478	nummod	39,40
R7655	T11480	T11478	punct	",",40
R7656	T11481	T11478	punct	),40
R7657	T11482	T11450	punct	.,require
R7658	T11484	T11485	nummod	One,finding
R7659	T11485	T11487	nsubj	finding,is
R7660	T11486	T11485	amod	unexpected,finding
R7661	T11488	T11485	prep	of,finding
R7662	T11489	T11490	det	this,study
R7663	T11490	T11488	pobj	study,of
R7664	T11491	T11492	amod	low,activity
R7665	T11492	T11487	attr	activity,is
R7666	T11493	T11492	compound	helicase,activity
R7667	T11494	T11492	prep	of,activity
R7668	T11495	T11494	pobj	Mcm4,of
R7669	T11496	T11495	punct	/,Mcm4
R7670	T11497	T11495	nummod	6,Mcm4
R7671	T11498	T11495	punct	/,Mcm4
R7672	T11499	T11495	nummod	7,Mcm4
R7673	T11500	T11492	prep	on,activity
R7674	T11501	T11502	npadvmod	GC,rich
R7675	T11502	T11504	amod	rich,DNA
R7676	T11503	T11502	punct	-,rich
R7677	T11504	T11500	pobj	DNA,on
R7678	T11505	T11504	compound	duplex,DNA
R7679	T11506	T11487	punct	.,is
R7680	T11508	T11509	nsubj	This,indicates
R7681	T11510	T11508	punct	", ",This
R7682	T11511	T11508	prep	in,This
R7683	T11512	T11511	pobj	conjunction,in
R7684	T11513	T11512	prep	with,conjunction
R7685	T11514	T11515	det	the,processivity
R7686	T11515	T11513	pobj	processivity,with
R7687	T11516	T11515	amod	low,processivity
R7688	T11517	T11515	prep	of,processivity
R7689	T11518	T11519	nmod	Mcm4,helicase
R7690	T11519	T11517	pobj	helicase,of
R7691	T11520	T11518	punct	/,Mcm4
R7692	T11521	T11518	nummod	6,Mcm4
R7693	T11522	T11518	punct	/,Mcm4
R7694	T11523	T11518	nummod	7,Mcm4
R7695	T11524	T11509	punct	", ",indicates
R7696	T11525	T11509	advmod	strongly,indicates
R7697	T11526	T11527	mark	that,require
R7698	T11527	T11509	ccomp	require,indicates
R7699	T11528	T11529	det	the,helicase
R7700	T11529	T11527	nsubj	helicase,require
R7701	T11530	T11529	amod	replicative,helicase
R7702	T11531	T11529	prep	at,helicase
R7703	T11532	T11533	det	the,forks
R7704	T11533	T11531	pobj	forks,at
R7705	T11534	T11533	compound	replication,forks
R7706	T11535	T11527	aux	would,require
R7707	T11536	T11527	dobj	more,require
R7708	T11537	T11536	prep	than,more
R7709	T11538	T11539	nmod	Mcm4,complex
R7710	T11539	T11537	pobj	complex,than
R7711	T11540	T11538	punct	/,Mcm4
R7712	T11541	T11538	nummod	6,Mcm4
R7713	T11542	T11538	punct	/,Mcm4
R7714	T11543	T11538	nummod	7,Mcm4
R7715	T11544	T11509	punct	.,indicates
R7716	T11546	T11547	prep	During,stimulated
R7717	T11548	T11549	det	the,unwinding
R7718	T11549	T11546	pobj	unwinding,During
R7719	T11550	T11549	amod	processive,unwinding
R7720	T11551	T11549	prep	of,unwinding
R7721	T11552	T11553	compound	duplex,forks
R7722	T11553	T11551	pobj	forks,of
R7723	T11554	T11553	compound	DNA,forks
R7724	T11555	T11553	compound	replication,forks
R7725	T11556	T11547	punct	", ",stimulated
R7726	T11557	T11547	nsubjpass	Mcm4,stimulated
R7727	T11558	T11557	punct	/,Mcm4
R7728	T11559	T11557	nummod	6,Mcm4
R7729	T11560	T11557	punct	/,Mcm4
R7730	T11561	T11557	nummod	7,Mcm4
R7731	T11562	T11547	aux	may,stimulated
R7732	T11563	T11547	auxpass	be,stimulated
R7733	T11564	T11547	advmod	further,stimulated
R7734	T11565	T11547	agent	by,stimulated
R7735	T11566	T11565	pobj	interaction,by
R7736	T11567	T11566	prep	with,interaction
R7737	T11568	T11569	amod	other,proteins
R7738	T11569	T11567	pobj	proteins,with
R7739	T11570	T11569	punct	", ",proteins
R7740	T11571	T11569	prep	including,proteins
R7741	T11572	T11573	amod	remaining,subunits
R7742	T11573	T11571	pobj	subunits,including
R7743	T11574	T11573	compound	Mcm,subunits
R7744	T11575	T11573	punct	", ",subunits
R7745	T11576	T11573	conj	Cdc45,subunits
R7746	T11577	T11578	punct	(,38
R7747	T11578	T11576	parataxis	38,Cdc45
R7748	T11579	T11578	nummod	37,38
R7749	T11580	T11578	punct	",",38
R7750	T11581	T11578	punct	),38
R7751	T11582	T11576	punct	", ",Cdc45
R7752	T11583	T11576	conj	GINS,Cdc45
R7753	T11584	T11585	punct	(,42
R7754	T11585	T11583	parataxis	42,GINS
R7755	T11586	T11585	nummod	41,42
R7756	T11587	T11585	punct	",",42
R7757	T11588	T11585	punct	),42
R7758	T11589	T11583	punct	", ",GINS
R7759	T11590	T11591	compound	DNA,subunits
R7760	T11591	T11583	conj	subunits,GINS
R7761	T11592	T11591	compound	polymerase,subunits
R7762	T11593	T11594	punct	(,43
R7763	T11594	T11591	parataxis	43,subunits
R7764	T11595	T11594	punct	),43
R7765	T11596	T11591	punct	", ",subunits
R7766	T11597	T11591	conj	Mcm8,subunits
R7767	T11598	T11599	punct	(,36
R7768	T11599	T11597	parataxis	36,Mcm8
R7769	T11600	T11599	nummod	35,36
R7770	T11601	T11599	punct	",",36
R7771	T11602	T11599	punct	),36
R7772	T11603	T11597	punct	", ",Mcm8
R7773	T11604	T11597	conj	etc.,Mcm8
R7774	T11605	T11606	aux	to,become
R7775	T11606	T11547	advcl	become,stimulated
R7776	T11607	T11608	det	a,helicase
R7777	T11608	T11606	attr	helicase,become
R7778	T11609	T11610	advmod	truly,processive
R7779	T11610	T11608	amod	processive,helicase
R7780	T11611	T11610	cc	and,processive
R7781	T11612	T11610	conj	potent,processive
R7782	T11613	T11608	amod	capable,helicase
R7783	T11614	T11613	prep	of,capable
R7784	T11615	T11614	pcomp	replicating,of
R7785	T11616	T11617	det	the,genome
R7786	T11617	T11615	dobj	genome,replicating
R7787	T11618	T11617	amod	entire,genome
R7788	T11619	T11547	punct	.,stimulated

PMC:1140370 / 28060-37852 JSONTXT 6 Projects

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PMC:1140370 / 28060-37852 JSON TXT 6 Projects