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PMC:1140370 / 24575-28058 JSONTXT 5 Projects

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Id Subject Object Predicate Lexical cue
T7722 0-3 NN denotes DNA
T7723 4-13 NN denotes unwinding
T7725 14-16 IN denotes by
T7726 17-21 NN denotes Mcm4
T7727 21-22 HYPH denotes /
T7728 22-23 CD denotes 6
T7729 23-24 HYPH denotes /
T7730 24-25 CD denotes 7
T7731 26-28 VBZ denotes is
T7724 29-38 VBN denotes inhibited
T7732 39-41 IN denotes by
T7733 42-45 DT denotes the
T7735 46-48 NN denotes GC
T7737 48-49 HYPH denotes -
T7736 49-53 JJ denotes rich
T7734 54-63 NNS denotes sequences
T7738 64-66 IN denotes on
T7739 67-70 DT denotes the
T7741 71-77 NN denotes duplex
T7740 78-85 NN denotes segment
T7742 85-212 sentence denotes Our results indicate that thymine-rich single-stranded DNA is required for initial loading and activation of the Mcm helicase.
T7743 86-89 PRP$ denotes Our
T7744 90-97 NNS denotes results
T7745 98-106 VBP denotes indicate
T7746 107-111 IN denotes that
T7748 112-119 NN denotes thymine
T7750 119-120 HYPH denotes -
T7749 120-124 JJ denotes rich
T7752 125-131 JJ denotes single
T7754 131-132 HYPH denotes -
T7753 132-140 VBN denotes stranded
T7751 141-144 NN denotes DNA
T7755 145-147 VBZ denotes is
T7747 148-156 VBN denotes required
T7756 157-160 IN denotes for
T7757 161-168 JJ denotes initial
T7758 169-176 NN denotes loading
T7759 177-180 CC denotes and
T7760 181-191 NN denotes activation
T7761 192-194 IN denotes of
T7762 195-198 DT denotes the
T7764 199-202 NN denotes Mcm
T7763 203-211 NN denotes helicase
T7765 211-212 . denotes .
T7766 212-316 sentence denotes However, it is not known whether thymine sequences are required for processive unwinding of duplex DNA.
T7767 213-220 RB denotes However
T7769 220-222 , denotes ,
T7770 222-224 PRP denotes it
T7771 225-227 VBZ denotes is
T7772 228-231 RB denotes not
T7768 232-237 VBN denotes known
T7773 238-245 IN denotes whether
T7775 246-253 NN denotes thymine
T7776 254-263 NNS denotes sequences
T7777 264-267 VBP denotes are
T7774 268-276 VBN denotes required
T7778 277-280 IN denotes for
T7779 281-291 JJ denotes processive
T7780 292-301 NN denotes unwinding
T7781 302-304 IN denotes of
T7782 305-311 NN denotes duplex
T7783 312-315 NN denotes DNA
T7784 315-316 . denotes .
T7785 316-432 sentence denotes Therefore, we have examined whether the nucleotide composition of the duplex region affects its unwinding activity.
T7786 317-326 RB denotes Therefore
T7788 326-328 , denotes ,
T7789 328-330 PRP denotes we
T7790 331-335 VBP denotes have
T7787 336-344 VBN denotes examined
T7791 345-352 IN denotes whether
T7793 353-356 DT denotes the
T7795 357-367 NN denotes nucleotide
T7794 368-379 NN denotes composition
T7796 380-382 IN denotes of
T7797 383-386 DT denotes the
T7799 387-393 NN denotes duplex
T7798 394-400 NN denotes region
T7792 401-408 VBZ denotes affects
T7800 409-412 PRP$ denotes its
T7802 413-422 NN denotes unwinding
T7801 423-431 NN denotes activity
T7803 431-432 . denotes .
T7804 432-570 sentence denotes To address this issue, we constructed a series of T-tailed Y-fork structures (T-fork) containing various sequences in the duplex segment.
T7805 433-435 TO denotes To
T7806 436-443 VB denotes address
T7808 444-448 DT denotes this
T7809 449-454 NN denotes issue
T7810 454-456 , denotes ,
T7811 456-458 PRP denotes we
T7807 459-470 VBD denotes constructed
T7812 471-472 DT denotes a
T7813 473-479 NN denotes series
T7814 480-482 IN denotes of
T7815 483-484 NN denotes T
T7817 484-485 HYPH denotes -
T7816 485-491 VBN denotes tailed
T7819 492-493 NN denotes Y
T7821 493-494 HYPH denotes -
T7820 494-498 NN denotes fork
T7818 499-509 NNS denotes structures
T7822 510-511 -LRB- denotes (
T7823 511-512 NN denotes T
T7825 512-513 HYPH denotes -
T7824 513-517 NN denotes fork
T7826 517-518 -RRB- denotes )
T7827 519-529 VBG denotes containing
T7828 530-537 JJ denotes various
T7829 538-547 NNS denotes sequences
T7830 548-550 IN denotes in
T7831 551-554 DT denotes the
T7833 555-561 NN denotes duplex
T7832 562-569 NN denotes segment
T7834 569-570 . denotes .
T7835 570-643 sentence denotes They carried varied contents of cytosine residues on the 3′-tail strand.
T7836 571-575 PRP denotes They
T7837 576-583 VBD denotes carried
T7838 584-590 VBN denotes varied
T7839 591-599 NNS denotes contents
T7840 600-602 IN denotes of
T7841 603-611 NN denotes cytosine
T7842 612-620 NNS denotes residues
T7843 621-623 IN denotes on
T7844 624-627 DT denotes the
T7846 628-629 CD denotes 3
T7848 629-630 SYM denotes ′
T7849 630-631 HYPH denotes -
T7847 631-635 NN denotes tail
T7845 636-642 NN denotes strand
T7850 642-643 . denotes .
T7851 643-820 sentence denotes In gel shift assays, Mcm4/6/7 bound to these Y-fork substrates with identical affinity (Figure 8A), consistent with the notion that the Mcm4/6/7 binds to single-stranded tails.
T7852 644-646 IN denotes In
T7854 647-650 NN denotes gel
T7855 651-656 NN denotes shift
T7856 657-663 NNS denotes assays
T7857 663-665 , denotes ,
T7858 665-669 NN denotes Mcm4
T7859 669-670 HYPH denotes /
T7860 670-671 CD denotes 6
T7861 671-672 HYPH denotes /
T7862 672-673 CD denotes 7
T7853 674-679 VBD denotes bound
T7863 680-682 IN denotes to
T7864 683-688 DT denotes these
T7866 689-690 NN denotes Y
T7868 690-691 HYPH denotes -
T7867 691-695 NN denotes fork
T7865 696-706 NNS denotes substrates
T7869 707-711 IN denotes with
T7870 712-721 JJ denotes identical
T7871 722-730 NN denotes affinity
T7872 731-732 -LRB- denotes (
T7874 732-738 NN denotes Figure
T7873 739-741 NN denotes 8A
T7875 741-742 -RRB- denotes )
T7876 742-744 , denotes ,
T7877 744-754 JJ denotes consistent
T7878 755-759 IN denotes with
T7879 760-763 DT denotes the
T7880 764-770 NN denotes notion
T7881 771-775 IN denotes that
T7883 776-779 DT denotes the
T7884 780-784 NN denotes Mcm4
T7885 784-785 HYPH denotes /
T7886 785-786 CD denotes 6
T7887 786-787 HYPH denotes /
T7888 787-788 CD denotes 7
T7882 789-794 VBZ denotes binds
T7889 795-797 IN denotes to
T7890 798-804 JJ denotes single
T7892 804-805 HYPH denotes -
T7891 805-813 VBN denotes stranded
T7893 814-819 NNS denotes tails
T7894 819-820 . denotes .
T7895 820-972 sentence denotes However, in DNA helicase assays, T-fork/(C:G)49 was hardly displaced by Mcm4/6/7, but was readily displaced by SV40 T-antigen DNA helicase (Figure 8B).
T7896 821-828 RB denotes However
T7898 828-830 , denotes ,
T7899 830-832 IN denotes in
T7900 833-836 NN denotes DNA
T7901 837-845 NN denotes helicase
T7902 846-852 NNS denotes assays
T7903 852-854 , denotes ,
T7904 854-855 NN denotes T
T7906 855-856 HYPH denotes -
T7905 856-860 NN denotes fork
T7907 860-861 HYPH denotes /
T7908 861-862 -LRB- denotes (
T7910 862-863 NN denotes C
T7911 863-864 : denotes :
T7909 864-865 NN denotes G
T7912 865-866 -RRB- denotes )
T7913 866-868 CD denotes 49
T7914 869-872 VBD denotes was
T7915 873-879 RB denotes hardly
T7897 880-889 VBN denotes displaced
T7916 890-892 IN denotes by
T7917 893-897 NN denotes Mcm4
T7918 897-898 HYPH denotes /
T7919 898-899 CD denotes 6
T7920 899-900 HYPH denotes /
T7921 900-901 CD denotes 7
T7922 901-903 , denotes ,
T7923 903-906 CC denotes but
T7924 907-910 VBD denotes was
T7926 911-918 RB denotes readily
T7925 919-928 VBN denotes displaced
T7927 929-931 IN denotes by
T7928 932-936 NN denotes SV40
T7930 937-938 NN denotes T
T7932 938-939 HYPH denotes -
T7931 939-946 NN denotes antigen
T7933 947-950 NN denotes DNA
T7929 951-959 NN denotes helicase
T7934 960-961 -LRB- denotes (
T7936 961-967 NN denotes Figure
T7935 968-970 NN denotes 8B
T7937 970-971 -RRB- denotes )
T7938 971-972 . denotes .
T7939 972-1138 sentence denotes When thymine or adenine is inserted as every third nucleotide (repeats of CCT or CCA), the extent of unwinding increased (T-fork/(CCT:GGA)16 and T-fork/(CCA:GGT)16).
T7940 973-977 WRB denotes When
T7942 978-985 NN denotes thymine
T7943 986-988 CC denotes or
T7944 989-996 NN denotes adenine
T7945 997-999 VBZ denotes is
T7941 1000-1008 VBN denotes inserted
T7947 1009-1011 IN denotes as
T7948 1012-1017 DT denotes every
T7950 1018-1023 JJ denotes third
T7949 1024-1034 NN denotes nucleotide
T7951 1035-1036 -LRB- denotes (
T7952 1036-1043 NNS denotes repeats
T7953 1044-1046 IN denotes of
T7954 1047-1050 NN denotes CCT
T7955 1051-1053 CC denotes or
T7956 1054-1057 NN denotes CCA
T7957 1057-1058 -RRB- denotes )
T7958 1058-1060 , denotes ,
T7959 1060-1063 DT denotes the
T7960 1064-1070 NN denotes extent
T7961 1071-1073 IN denotes of
T7962 1074-1083 NN denotes unwinding
T7946 1084-1093 VBD denotes increased
T7963 1094-1095 -LRB- denotes (
T7965 1095-1096 NN denotes T
T7967 1096-1097 HYPH denotes -
T7966 1097-1101 NN denotes fork
T7968 1101-1102 HYPH denotes /
T7969 1102-1103 -LRB- denotes (
T7970 1103-1106 NN denotes CCT
T7971 1106-1107 : denotes :
T7964 1107-1110 NN denotes GGA
T7972 1110-1111 -RRB- denotes )
T7973 1111-1113 CD denotes 16
T7974 1114-1117 CC denotes and
T7975 1118-1119 NN denotes T
T7977 1119-1120 HYPH denotes -
T7976 1120-1124 NN denotes fork
T7979 1124-1125 HYPH denotes /
T7980 1125-1126 -LRB- denotes (
T7981 1126-1129 NN denotes CCA
T7982 1129-1130 : denotes :
T7978 1130-1133 NN denotes GGT
T7983 1133-1134 -RRB- denotes )
T7984 1134-1136 CD denotes 16
T7985 1136-1137 -RRB- denotes )
T7986 1137-1138 . denotes .
T7987 1138-1343 sentence denotes The efficiency of unwinding is roughly correlated with the content of GC pairs in the duplex segment [T-fork/(CCAA:GGTT)12, T-fork/(CCTT:GGAA)12, T-fork/(CAAA:GTTT)12 and T-fork/(CTTT:GAAA)12; Figure 8B].
T7988 1139-1142 DT denotes The
T7989 1143-1153 NN denotes efficiency
T7991 1154-1156 IN denotes of
T7992 1157-1166 NN denotes unwinding
T7993 1167-1169 VBZ denotes is
T7994 1170-1177 RB denotes roughly
T7990 1178-1188 VBN denotes correlated
T7995 1189-1193 IN denotes with
T7996 1194-1197 DT denotes the
T7997 1198-1205 NN denotes content
T7998 1206-1208 IN denotes of
T7999 1209-1211 NN denotes GC
T8000 1212-1217 NNS denotes pairs
T8001 1218-1220 IN denotes in
T8002 1221-1224 DT denotes the
T8004 1225-1231 NN denotes duplex
T8003 1232-1239 NN denotes segment
T8005 1240-1241 -LRB- denotes [
T8007 1241-1242 NN denotes T
T8009 1242-1243 HYPH denotes -
T8008 1243-1247 NN denotes fork
T8010 1247-1248 HYPH denotes /
T8011 1248-1249 -LRB- denotes (
T8012 1249-1253 NN denotes CCAA
T8013 1253-1254 : denotes :
T8006 1254-1258 NN denotes GGTT
T8014 1258-1259 -RRB- denotes )
T8015 1259-1261 CD denotes 12
T8016 1261-1263 , denotes ,
T8017 1263-1264 NN denotes T
T8019 1264-1265 HYPH denotes -
T8018 1265-1269 NN denotes fork
T8021 1269-1270 HYPH denotes /
T8022 1270-1271 -LRB- denotes (
T8023 1271-1275 NN denotes CCTT
T8024 1275-1276 : denotes :
T8020 1276-1280 NN denotes GGAA
T8025 1280-1281 -RRB- denotes )
T8026 1281-1283 CD denotes 12
T8027 1283-1285 , denotes ,
T8028 1285-1286 NN denotes T
T8030 1286-1287 HYPH denotes -
T8029 1287-1291 NN denotes fork
T8032 1291-1292 HYPH denotes /
T8033 1292-1293 -LRB- denotes (
T8034 1293-1297 NN denotes CAAA
T8035 1297-1298 : denotes :
T8031 1298-1302 NN denotes GTTT
T8036 1302-1303 -RRB- denotes )
T8037 1303-1305 CD denotes 12
T8038 1306-1309 CC denotes and
T8039 1310-1311 NN denotes T
T8041 1311-1312 HYPH denotes -
T8040 1312-1316 NN denotes fork
T8043 1316-1317 HYPH denotes /
T8044 1317-1318 -LRB- denotes (
T8045 1318-1322 NN denotes CTTT
T8046 1322-1323 : denotes :
T8042 1323-1327 NN denotes GAAA
T8047 1327-1328 -RRB- denotes )
T8048 1328-1330 CD denotes 12
T8049 1330-1331 : denotes ;
T8051 1332-1338 NN denotes Figure
T8050 1339-1341 NN denotes 8B
T8052 1341-1342 -RRB- denotes ]
T8053 1342-1343 . denotes .
T8054 1343-1439 sentence denotes It appears that the duplex segment containing <50% GC pairs is efficiently unwound (Figure 8C).
T8055 1344-1346 PRP denotes It
T8056 1347-1354 VBZ denotes appears
T8057 1355-1359 IN denotes that
T8059 1360-1363 DT denotes the
T8061 1364-1370 NN denotes duplex
T8060 1371-1378 NN denotes segment
T8062 1379-1389 VBG denotes containing
T8063 1390-1391 SYM denotes <
T8064 1391-1393 CD denotes 50
T8065 1393-1394 NN denotes %
T8067 1395-1397 NN denotes GC
T8066 1398-1403 NNS denotes pairs
T8068 1404-1406 VBZ denotes is
T8069 1407-1418 RB denotes efficiently
T8058 1419-1426 VBN denotes unwound
T8070 1427-1428 -LRB- denotes (
T8072 1428-1434 NN denotes Figure
T8071 1435-1437 NN denotes 8C
T8073 1437-1438 -RRB- denotes )
T8074 1438-1439 . denotes .
T8075 1439-1552 sentence denotes We next examined the effect of GC-rich segments on a partial heteroduplex DNA on a single-stranded circular DNA.
T8076 1440-1442 PRP denotes We
T8078 1443-1447 RB denotes next
T8077 1448-1456 VBD denotes examined
T8079 1457-1460 DT denotes the
T8080 1461-1467 NN denotes effect
T8081 1468-1470 IN denotes of
T8082 1471-1473 NN denotes GC
T8084 1473-1474 HYPH denotes -
T8083 1474-1478 JJ denotes rich
T8085 1479-1487 NNS denotes segments
T8086 1488-1490 IN denotes on
T8087 1491-1492 DT denotes a
T8089 1493-1500 JJ denotes partial
T8090 1501-1513 NN denotes heteroduplex
T8088 1514-1517 NN denotes DNA
T8091 1518-1520 IN denotes on
T8092 1521-1522 DT denotes a
T8094 1523-1529 JJ denotes single
T8096 1529-1530 HYPH denotes -
T8095 1530-1538 VBN denotes stranded
T8097 1539-1547 JJ denotes circular
T8093 1548-1551 NN denotes DNA
T8098 1551-1552 . denotes .
T8099 1552-1810 sentence denotes We have constructed two sets of 5′-tailed partial heteroduplex DNA substrates containing duplex regions of variable lengths; one on M13mp18 vector and the other on M13mp19 containing a 66 nt long G-rich segment downstream of the hybridizing oligonucleotide.
T8100 1553-1555 PRP denotes We
T8102 1556-1560 VBP denotes have
T8101 1561-1572 VBN denotes constructed
T8103 1573-1576 CD denotes two
T8104 1577-1581 NNS denotes sets
T8105 1582-1584 IN denotes of
T8106 1585-1586 CD denotes 5
T8108 1586-1587 SYM denotes ′
T8109 1587-1588 HYPH denotes -
T8107 1588-1594 VBN denotes tailed
T8111 1595-1602 JJ denotes partial
T8112 1603-1615 NN denotes heteroduplex
T8113 1616-1619 NN denotes DNA
T8110 1620-1630 NNS denotes substrates
T8114 1631-1641 VBG denotes containing
T8115 1642-1648 NN denotes duplex
T8116 1649-1656 NNS denotes regions
T8117 1657-1659 IN denotes of
T8118 1660-1668 JJ denotes variable
T8119 1669-1676 NNS denotes lengths
T8120 1676-1677 : denotes ;
T8121 1678-1681 CD denotes one
T8122 1682-1684 IN denotes on
T8123 1685-1692 NN denotes M13mp18
T8124 1693-1699 NN denotes vector
T8125 1700-1703 CC denotes and
T8126 1704-1707 DT denotes the
T8127 1708-1713 JJ denotes other
T8128 1714-1716 IN denotes on
T8129 1717-1724 NN denotes M13mp19
T8130 1725-1735 VBG denotes containing
T8131 1736-1737 DT denotes a
T8133 1738-1740 CD denotes 66
T8134 1741-1743 NN denotes nt
T8135 1744-1748 JJ denotes long
T8136 1749-1750 NN denotes G
T8138 1750-1751 HYPH denotes -
T8137 1751-1755 JJ denotes rich
T8132 1756-1763 NN denotes segment
T8139 1764-1774 RB denotes downstream
T8140 1775-1777 IN denotes of
T8141 1778-1781 DT denotes the
T8143 1782-1793 VBG denotes hybridizing
T8142 1794-1809 NN denotes oligonucleotide
T8144 1809-1810 . denotes .
T8145 1810-1981 sentence denotes We found that the Mcm4/6/7 helicase can displace duplex DNA up to 350 nt long on its own on the former substrate (Figure 8D, left), consistent with previous results (13).
T8146 1811-1813 PRP denotes We
T8147 1814-1819 VBD denotes found
T8148 1820-1824 IN denotes that
T8150 1825-1828 DT denotes the
T8152 1829-1833 NN denotes Mcm4
T8153 1833-1834 HYPH denotes /
T8154 1834-1835 CD denotes 6
T8155 1835-1836 HYPH denotes /
T8156 1836-1837 CD denotes 7
T8151 1838-1846 NN denotes helicase
T8157 1847-1850 MD denotes can
T8149 1851-1859 VB denotes displace
T8158 1860-1866 NN denotes duplex
T8159 1867-1870 NN denotes DNA
T8160 1871-1873 IN denotes up
T8162 1874-1876 IN denotes to
T8161 1877-1880 CD denotes 350
T8163 1881-1883 NN denotes nt
T8164 1884-1888 JJ denotes long
T8165 1889-1891 IN denotes on
T8166 1892-1895 PRP$ denotes its
T8167 1896-1899 JJ denotes own
T8168 1900-1902 IN denotes on
T8169 1903-1906 DT denotes the
T8171 1907-1913 JJ denotes former
T8170 1914-1923 NN denotes substrate
T8172 1924-1925 -LRB- denotes (
T8174 1925-1931 NN denotes Figure
T8173 1932-1934 NN denotes 8D
T8175 1934-1936 , denotes ,
T8176 1936-1940 JJ denotes left
T8177 1940-1941 -RRB- denotes )
T8178 1941-1943 , denotes ,
T8179 1943-1953 JJ denotes consistent
T8180 1954-1958 IN denotes with
T8181 1959-1967 JJ denotes previous
T8182 1968-1975 NNS denotes results
T8183 1976-1977 -LRB- denotes (
T8184 1977-1979 CD denotes 13
T8185 1979-1980 -RRB- denotes )
T8186 1980-1981 . denotes .
T8187 1981-2228 sentence denotes In contrast, displacement was inhibited over the GC-rich segments on the latter substrate (Figure 8D, right panel), although displacement up to 250 nt in length, albeit at a reduced level, was observed at a high concentration of Mcm4/6/7 complex.
T8188 1982-1984 IN denotes In
T8190 1985-1993 NN denotes contrast
T8191 1993-1995 , denotes ,
T8192 1995-2007 NN denotes displacement
T8193 2008-2011 VBD denotes was
T8189 2012-2021 VBN denotes inhibited
T8194 2022-2026 IN denotes over
T8195 2027-2030 DT denotes the
T8197 2031-2033 NN denotes GC
T8199 2033-2034 HYPH denotes -
T8198 2034-2038 JJ denotes rich
T8196 2039-2047 NNS denotes segments
T8200 2048-2050 IN denotes on
T8201 2051-2054 DT denotes the
T8203 2055-2061 JJ denotes latter
T8202 2062-2071 NN denotes substrate
T8204 2072-2073 -LRB- denotes (
T8206 2073-2079 NN denotes Figure
T8207 2080-2082 NN denotes 8D
T8208 2082-2084 , denotes ,
T8209 2084-2089 JJ denotes right
T8205 2090-2095 NN denotes panel
T8210 2095-2096 -RRB- denotes )
T8211 2096-2098 , denotes ,
T8212 2098-2106 IN denotes although
T8214 2107-2119 NN denotes displacement
T8215 2120-2122 IN denotes up
T8217 2123-2125 IN denotes to
T8216 2126-2129 CD denotes 250
T8218 2130-2132 NN denotes nt
T8219 2133-2135 IN denotes in
T8220 2136-2142 NN denotes length
T8221 2142-2144 , denotes ,
T8222 2144-2150 IN denotes albeit
T8223 2151-2153 IN denotes at
T8224 2154-2155 DT denotes a
T8226 2156-2163 VBN denotes reduced
T8225 2164-2169 NN denotes level
T8227 2169-2171 , denotes ,
T8228 2171-2174 VBD denotes was
T8213 2175-2183 VBN denotes observed
T8229 2184-2186 IN denotes at
T8230 2187-2188 DT denotes a
T8232 2189-2193 JJ denotes high
T8231 2194-2207 NN denotes concentration
T8233 2208-2210 IN denotes of
T8234 2211-2215 NN denotes Mcm4
T8236 2215-2216 HYPH denotes /
T8237 2216-2217 CD denotes 6
T8238 2217-2218 HYPH denotes /
T8239 2218-2219 CD denotes 7
T8235 2220-2227 NN denotes complex
T8240 2227-2228 . denotes .
T8241 2228-2365 sentence denotes This result is consistent with that on Y-fork and indicates that GC-rich duplex segment is inhibitory for unwinding by the Mcm helicase.
T8242 2229-2233 DT denotes This
T8243 2234-2240 NN denotes result
T8244 2241-2243 VBZ denotes is
T8245 2244-2254 JJ denotes consistent
T8246 2255-2259 IN denotes with
T8247 2260-2264 DT denotes that
T8248 2265-2267 IN denotes on
T8249 2268-2269 NN denotes Y
T8251 2269-2270 HYPH denotes -
T8250 2270-2274 NN denotes fork
T8252 2275-2278 CC denotes and
T8253 2279-2288 VBZ denotes indicates
T8254 2289-2293 IN denotes that
T8256 2294-2296 NN denotes GC
T8258 2296-2297 HYPH denotes -
T8257 2297-2301 JJ denotes rich
T8260 2302-2308 NN denotes duplex
T8259 2309-2316 NN denotes segment
T8255 2317-2319 VBZ denotes is
T8261 2320-2330 JJ denotes inhibitory
T8262 2331-2334 IN denotes for
T8263 2335-2344 NN denotes unwinding
T8264 2345-2347 IN denotes by
T8265 2348-2351 DT denotes the
T8267 2352-2355 NN denotes Mcm
T8266 2356-2364 NN denotes helicase
T8268 2364-2365 . denotes .
T8269 2365-2530 sentence denotes These results may indicate that the presence of thymine–adenine pairs with a certain frequency in the duplex region may facilitate continuous unwinding by Mcm4/6/7.
T8270 2366-2371 DT denotes These
T8271 2372-2379 NNS denotes results
T8273 2380-2383 MD denotes may
T8272 2384-2392 VB denotes indicate
T8274 2393-2397 IN denotes that
T8276 2398-2401 DT denotes the
T8277 2402-2410 NN denotes presence
T8278 2411-2413 IN denotes of
T8279 2414-2421 NN denotes thymine
T8281 2421-2422 HYPH denotes –
T8280 2422-2429 NN denotes adenine
T8282 2430-2435 NNS denotes pairs
T8283 2436-2440 IN denotes with
T8284 2441-2442 DT denotes a
T8286 2443-2450 JJ denotes certain
T8285 2451-2460 NN denotes frequency
T8287 2461-2463 IN denotes in
T8288 2464-2467 DT denotes the
T8290 2468-2474 NN denotes duplex
T8289 2475-2481 NN denotes region
T8291 2482-2485 MD denotes may
T8275 2486-2496 VB denotes facilitate
T8292 2497-2507 JJ denotes continuous
T8293 2508-2517 NN denotes unwinding
T8294 2518-2520 IN denotes by
T8295 2521-2525 NN denotes Mcm4
T8296 2525-2526 HYPH denotes /
T8297 2526-2527 CD denotes 6
T8298 2527-2528 HYPH denotes /
T8299 2528-2529 CD denotes 7
T8300 2529-2530 . denotes .
T8301 2530-2639 sentence denotes Alternatively, Mcm4/6/7 is not efficient enough to displace thermodynamically stable GC-base-paired segment.
T8302 2531-2544 RB denotes Alternatively
T8304 2544-2546 , denotes ,
T8305 2546-2550 NN denotes Mcm4
T8306 2550-2551 HYPH denotes /
T8307 2551-2552 CD denotes 6
T8308 2552-2553 HYPH denotes /
T8309 2553-2554 CD denotes 7
T8303 2555-2557 VBZ denotes is
T8310 2558-2561 RB denotes not
T8311 2562-2571 JJ denotes efficient
T8312 2572-2578 RB denotes enough
T8313 2579-2581 TO denotes to
T8314 2582-2590 VB denotes displace
T8315 2591-2608 RB denotes thermodynamically
T8316 2609-2615 JJ denotes stable
T8318 2616-2618 NN denotes GC
T8320 2618-2619 HYPH denotes -
T8321 2619-2623 NN denotes base
T8322 2623-2624 HYPH denotes -
T8319 2624-2630 VBN denotes paired
T8317 2631-2638 NN denotes segment
T8323 2638-2639 . denotes .
T8324 2639-2784 sentence denotes In order to distinguish these two possibilities, we designed a new T-fork substrate containing inosine (I) residue instead of guanosine residue.
T8325 2640-2642 IN denotes In
T8327 2643-2648 NN denotes order
T8328 2649-2651 TO denotes to
T8329 2652-2663 VB denotes distinguish
T8330 2664-2669 DT denotes these
T8332 2670-2673 CD denotes two
T8331 2674-2687 NNS denotes possibilities
T8333 2687-2689 , denotes ,
T8334 2689-2691 PRP denotes we
T8326 2692-2700 VBD denotes designed
T8335 2701-2702 DT denotes a
T8337 2703-2706 JJ denotes new
T8338 2707-2708 NN denotes T
T8340 2708-2709 HYPH denotes -
T8339 2709-2713 NN denotes fork
T8336 2714-2723 NN denotes substrate
T8341 2724-2734 VBG denotes containing
T8342 2735-2742 NN denotes inosine
T8344 2743-2744 -LRB- denotes (
T8345 2744-2745 NN denotes I
T8346 2745-2746 -RRB- denotes )
T8343 2747-2754 NN denotes residue
T8347 2755-2762 RB denotes instead
T8348 2763-2765 IN denotes of
T8349 2766-2775 NN denotes guanosine
T8350 2776-2783 NN denotes residue
T8351 2783-2784 . denotes .
T8352 2784-2957 sentence denotes The thermal stability of the I:C base pair is lower than that of the G:C base pair in duplex DNA due to loss of one hydrogen bond and is even lower than A:T base pair (31).
T8353 2785-2788 DT denotes The
T8355 2789-2796 JJ denotes thermal
T8354 2797-2806 NN denotes stability
T8357 2807-2809 IN denotes of
T8358 2810-2813 DT denotes the
T8360 2814-2815 NN denotes I
T8362 2815-2816 : denotes :
T8361 2816-2817 NN denotes C
T8363 2818-2822 NN denotes base
T8359 2823-2827 NN denotes pair
T8356 2828-2830 VBZ denotes is
T8364 2831-2836 JJR denotes lower
T8365 2837-2841 IN denotes than
T8366 2842-2846 DT denotes that
T8367 2847-2849 IN denotes of
T8368 2850-2853 DT denotes the
T8370 2854-2855 NN denotes G
T8372 2855-2856 : denotes :
T8371 2856-2857 NN denotes C
T8373 2858-2862 NN denotes base
T8369 2863-2867 NN denotes pair
T8374 2868-2870 IN denotes in
T8375 2871-2877 NN denotes duplex
T8376 2878-2881 NN denotes DNA
T8377 2882-2885 IN denotes due
T8378 2886-2888 IN denotes to
T8379 2889-2893 NN denotes loss
T8380 2894-2896 IN denotes of
T8381 2897-2900 CD denotes one
T8383 2901-2909 NN denotes hydrogen
T8382 2910-2914 NN denotes bond
T8384 2915-2918 CC denotes and
T8385 2919-2921 VBZ denotes is
T8386 2922-2926 RB denotes even
T8387 2927-2932 JJR denotes lower
T8388 2933-2937 IN denotes than
T8389 2938-2939 NN denotes A
T8391 2939-2940 : denotes :
T8390 2940-2941 NN denotes T
T8393 2942-2946 NN denotes base
T8392 2947-2951 NN denotes pair
T8394 2952-2953 -LRB- denotes (
T8395 2953-2955 CD denotes 31
T8396 2955-2956 -RRB- denotes )
T8397 2956-2957 . denotes .
T8398 2957-3088 sentence denotes We constructed the T-tailed fork substrates containing the (GCC:CGG)10, (GCC:CIG)10 or (GAA:CTT)10 duplex DNA segment (Figure 8E).
T8399 2958-2960 PRP denotes We
T8400 2961-2972 VBD denotes constructed
T8401 2973-2976 DT denotes the
T8403 2977-2978 NN denotes T
T8405 2978-2979 HYPH denotes -
T8404 2979-2985 VBN denotes tailed
T8406 2986-2990 NN denotes fork
T8402 2991-3001 NNS denotes substrates
T8407 3002-3012 VBG denotes containing
T8408 3013-3016 DT denotes the
T8410 3017-3018 -LRB- denotes (
T8412 3018-3021 NN denotes GCC
T8413 3021-3022 : denotes :
T8411 3022-3025 NN denotes CGG
T8414 3025-3026 -RRB- denotes )
T8415 3026-3028 CD denotes 10
T8416 3028-3030 , denotes ,
T8417 3030-3031 -LRB- denotes (
T8419 3031-3034 NN denotes GCC
T8420 3034-3035 : denotes :
T8418 3035-3038 NN denotes CIG
T8421 3038-3039 -RRB- denotes )
T8422 3039-3041 CD denotes 10
T8423 3042-3044 CC denotes or
T8424 3045-3046 -LRB- denotes (
T8426 3046-3049 NN denotes GAA
T8427 3049-3050 : denotes :
T8425 3050-3053 NN denotes CTT
T8428 3053-3054 -RRB- denotes )
T8429 3054-3056 CD denotes 10
T8430 3057-3063 NN denotes duplex
T8431 3064-3067 NN denotes DNA
T8409 3068-3075 NN denotes segment
T8432 3076-3077 -LRB- denotes (
T8434 3077-3083 NN denotes Figure
T8433 3084-3086 NN denotes 8E
T8435 3086-3087 -RRB- denotes )
T8436 3087-3088 . denotes .
T8437 3088-3234 sentence denotes Mcm4/6/7 hardly displaced the 31 nt long duplex of GCC:CGG repeats, whereas the T-fork/(GAA:CTT)10 was displaced efficiently, as described above.
T8438 3089-3093 NN denotes Mcm4
T8440 3093-3094 HYPH denotes /
T8441 3094-3095 CD denotes 6
T8442 3095-3096 HYPH denotes /
T8443 3096-3097 CD denotes 7
T8444 3098-3104 RB denotes hardly
T8439 3105-3114 VBD denotes displaced
T8445 3115-3118 DT denotes the
T8447 3119-3121 CD denotes 31
T8448 3122-3124 NN denotes nt
T8449 3125-3129 JJ denotes long
T8446 3130-3136 NN denotes duplex
T8450 3137-3139 IN denotes of
T8451 3140-3143 NN denotes GCC
T8453 3143-3144 : denotes :
T8452 3144-3147 NN denotes CGG
T8454 3148-3155 NNS denotes repeats
T8455 3155-3157 , denotes ,
T8456 3157-3164 IN denotes whereas
T8458 3165-3168 DT denotes the
T8460 3169-3170 NN denotes T
T8462 3170-3171 HYPH denotes -
T8461 3171-3175 NN denotes fork
T8463 3175-3176 HYPH denotes /
T8464 3176-3177 -LRB- denotes (
T8465 3177-3180 NN denotes GAA
T8466 3180-3181 : denotes :
T8459 3181-3184 NN denotes CTT
T8467 3184-3185 -RRB- denotes )
T8468 3185-3187 CD denotes 10
T8469 3188-3191 VBD denotes was
T8457 3192-3201 VBN denotes displaced
T8470 3202-3213 RB denotes efficiently
T8471 3213-3215 , denotes ,
T8472 3215-3217 IN denotes as
T8473 3218-3227 VBN denotes described
T8474 3228-3233 RB denotes above
T8475 3233-3234 . denotes .
T8476 3234-3483 sentence denotes T-fork/(GCC:CIG)10 was displaced with efficiency much greater than that of T-fork/(GCC:CGG)10, indicating that the thermostability of the duplex segment, not the lack of AT base pair, is responsible for inability of Mcm4/6/7 to displace the duplex.
T8477 3235-3236 NN denotes T
T8479 3236-3237 HYPH denotes -
T8478 3237-3241 NN denotes fork
T8481 3241-3242 HYPH denotes /
T8482 3242-3243 -LRB- denotes (
T8483 3243-3246 NN denotes GCC
T8484 3246-3247 : denotes :
T8480 3247-3250 NN denotes CIG
T8486 3250-3251 -RRB- denotes )
T8487 3251-3253 CD denotes 10
T8488 3254-3257 VBD denotes was
T8485 3258-3267 VBN denotes displaced
T8489 3268-3272 IN denotes with
T8490 3273-3283 NN denotes efficiency
T8491 3284-3288 RB denotes much
T8492 3289-3296 JJR denotes greater
T8493 3297-3301 IN denotes than
T8494 3302-3306 DT denotes that
T8495 3307-3309 IN denotes of
T8496 3310-3311 NN denotes T
T8498 3311-3312 HYPH denotes -
T8497 3312-3316 NN denotes fork
T8500 3316-3317 HYPH denotes /
T8501 3317-3318 -LRB- denotes (
T8502 3318-3321 NN denotes GCC
T8503 3321-3322 : denotes :
T8499 3322-3325 NN denotes CGG
T8504 3325-3326 -RRB- denotes )
T8505 3326-3328 CD denotes 10
T8506 3328-3330 , denotes ,
T8507 3330-3340 VBG denotes indicating
T8508 3341-3345 IN denotes that
T8510 3346-3349 DT denotes the
T8511 3350-3365 NN denotes thermostability
T8512 3366-3368 IN denotes of
T8513 3369-3372 DT denotes the
T8515 3373-3379 NN denotes duplex
T8514 3380-3387 NN denotes segment
T8516 3387-3389 , denotes ,
T8517 3389-3392 CC denotes not
T8518 3393-3396 DT denotes the
T8519 3397-3401 NN denotes lack
T8520 3402-3404 IN denotes of
T8521 3405-3407 NN denotes AT
T8523 3408-3412 NN denotes base
T8522 3413-3417 NN denotes pair
T8524 3417-3419 , denotes ,
T8509 3419-3421 VBZ denotes is
T8525 3422-3433 JJ denotes responsible
T8526 3434-3437 IN denotes for
T8527 3438-3447 NN denotes inability
T8528 3448-3450 IN denotes of
T8529 3451-3455 NN denotes Mcm4
T8530 3455-3456 HYPH denotes /
T8531 3456-3457 CD denotes 6
T8532 3457-3458 HYPH denotes /
T8533 3458-3459 CD denotes 7
T8534 3460-3462 TO denotes to
T8535 3463-3471 VB denotes displace
T8536 3472-3475 DT denotes the
T8537 3476-3482 NN denotes duplex
T8538 3482-3483 . denotes .
R5124 T7722 T7723 compound DNA,unwinding
R5125 T7723 T7724 nsubjpass unwinding,inhibited
R5126 T7725 T7723 prep by,unwinding
R5127 T7726 T7725 pobj Mcm4,by
R5128 T7727 T7726 punct /,Mcm4
R5129 T7728 T7726 nummod 6,Mcm4
R5130 T7729 T7726 punct /,Mcm4
R5131 T7730 T7726 nummod 7,Mcm4
R5132 T7731 T7724 auxpass is,inhibited
R5133 T7732 T7724 agent by,inhibited
R5134 T7733 T7734 det the,sequences
R5135 T7734 T7732 pobj sequences,by
R5136 T7735 T7736 npadvmod GC,rich
R5137 T7736 T7734 amod rich,sequences
R5138 T7737 T7736 punct -,rich
R5139 T7738 T7734 prep on,sequences
R5140 T7739 T7740 det the,segment
R5141 T7740 T7738 pobj segment,on
R5142 T7741 T7740 compound duplex,segment
R5143 T7743 T7744 poss Our,results
R5144 T7744 T7745 nsubj results,indicate
R5145 T7746 T7747 mark that,required
R5146 T7747 T7745 ccomp required,indicate
R5147 T7748 T7749 npadvmod thymine,rich
R5148 T7749 T7751 amod rich,DNA
R5149 T7750 T7749 punct -,rich
R5150 T7751 T7747 nsubjpass DNA,required
R5151 T7752 T7753 amod single,stranded
R5152 T7753 T7751 amod stranded,DNA
R5153 T7754 T7753 punct -,stranded
R5154 T7755 T7747 auxpass is,required
R5155 T7756 T7747 prep for,required
R5156 T7757 T7758 amod initial,loading
R5157 T7758 T7756 pobj loading,for
R5158 T7759 T7758 cc and,loading
R5159 T7760 T7758 conj activation,loading
R5160 T7761 T7758 prep of,loading
R5161 T7762 T7763 det the,helicase
R5162 T7763 T7761 pobj helicase,of
R5163 T7764 T7763 compound Mcm,helicase
R5164 T7765 T7745 punct .,indicate
R5165 T7767 T7768 advmod However,known
R5166 T7769 T7768 punct ", ",known
R5167 T7770 T7768 nsubjpass it,known
R5168 T7771 T7768 auxpass is,known
R5169 T7772 T7768 neg not,known
R5170 T7773 T7774 mark whether,required
R5171 T7774 T7768 ccomp required,known
R5172 T7775 T7776 compound thymine,sequences
R5173 T7776 T7774 nsubjpass sequences,required
R5174 T7777 T7774 auxpass are,required
R5175 T7778 T7774 prep for,required
R5176 T7779 T7780 amod processive,unwinding
R5177 T7780 T7778 pobj unwinding,for
R5178 T7781 T7780 prep of,unwinding
R5179 T7782 T7783 compound duplex,DNA
R5180 T7783 T7781 pobj DNA,of
R5181 T7784 T7768 punct .,known
R5182 T7786 T7787 advmod Therefore,examined
R5183 T7788 T7787 punct ", ",examined
R5184 T7789 T7787 nsubj we,examined
R5185 T7790 T7787 aux have,examined
R5186 T7791 T7792 mark whether,affects
R5187 T7792 T7787 ccomp affects,examined
R5188 T7793 T7794 det the,composition
R5189 T7794 T7792 nsubj composition,affects
R5190 T7795 T7794 compound nucleotide,composition
R5191 T7796 T7794 prep of,composition
R5192 T7797 T7798 det the,region
R5193 T7798 T7796 pobj region,of
R5194 T7799 T7798 compound duplex,region
R5195 T7800 T7801 poss its,activity
R5196 T7801 T7792 dobj activity,affects
R5197 T7802 T7801 compound unwinding,activity
R5198 T7803 T7787 punct .,examined
R5199 T7805 T7806 aux To,address
R5200 T7806 T7807 advcl address,constructed
R5201 T7808 T7809 det this,issue
R5202 T7809 T7806 dobj issue,address
R5203 T7810 T7807 punct ", ",constructed
R5204 T7811 T7807 nsubj we,constructed
R5205 T7812 T7813 det a,series
R5206 T7813 T7807 dobj series,constructed
R5207 T7814 T7813 prep of,series
R5208 T7815 T7816 npadvmod T,tailed
R5209 T7816 T7818 amod tailed,structures
R5210 T7817 T7816 punct -,tailed
R5211 T7818 T7814 pobj structures,of
R5212 T7819 T7820 compound Y,fork
R5213 T7820 T7818 compound fork,structures
R5214 T7821 T7820 punct -,fork
R5215 T7822 T7818 punct (,structures
R5216 T7823 T7824 compound T,fork
R5217 T7824 T7818 appos fork,structures
R5218 T7825 T7824 punct -,fork
R5219 T7826 T7818 punct ),structures
R5220 T7827 T7818 acl containing,structures
R5221 T7828 T7829 amod various,sequences
R5222 T7829 T7827 dobj sequences,containing
R5223 T7830 T7807 prep in,constructed
R5224 T7831 T7832 det the,segment
R5225 T7832 T7830 pobj segment,in
R5226 T7833 T7832 compound duplex,segment
R5227 T7834 T7807 punct .,constructed
R5228 T7836 T7837 nsubj They,carried
R5229 T7838 T7839 amod varied,contents
R5230 T7839 T7837 dobj contents,carried
R5231 T7840 T7839 prep of,contents
R5232 T7841 T7842 compound cytosine,residues
R5233 T7842 T7840 pobj residues,of
R5234 T7843 T7837 prep on,carried
R5235 T7844 T7845 det the,strand
R5236 T7845 T7843 pobj strand,on
R5237 T7846 T7847 nummod 3,tail
R5238 T7847 T7845 compound tail,strand
R5239 T7848 T7846 punct ′,3
R5240 T7849 T7847 punct -,tail
R5241 T7850 T7837 punct .,carried
R5242 T7852 T7853 prep In,bound
R5243 T7854 T7855 compound gel,shift
R5244 T7855 T7856 compound shift,assays
R5245 T7856 T7852 pobj assays,In
R5246 T7857 T7853 punct ", ",bound
R5247 T7858 T7853 nsubj Mcm4,bound
R5248 T7859 T7858 punct /,Mcm4
R5249 T7860 T7858 nummod 6,Mcm4
R5250 T7861 T7858 punct /,Mcm4
R5251 T7862 T7858 nummod 7,Mcm4
R5252 T7863 T7853 prep to,bound
R5253 T7864 T7865 det these,substrates
R5254 T7865 T7863 pobj substrates,to
R5255 T7866 T7867 compound Y,fork
R5256 T7867 T7865 compound fork,substrates
R5257 T7868 T7867 punct -,fork
R5258 T7869 T7853 prep with,bound
R5259 T7870 T7871 amod identical,affinity
R5260 T7871 T7869 pobj affinity,with
R5261 T7872 T7873 punct (,8A
R5262 T7873 T7871 parataxis 8A,affinity
R5263 T7874 T7873 compound Figure,8A
R5264 T7875 T7873 punct ),8A
R5265 T7876 T7853 punct ", ",bound
R5266 T7877 T7853 advcl consistent,bound
R5267 T7878 T7877 prep with,consistent
R5268 T7879 T7880 det the,notion
R5269 T7880 T7878 pobj notion,with
R5270 T7881 T7882 mark that,binds
R5271 T7882 T7880 acl binds,notion
R5272 T7883 T7884 det the,Mcm4
R5273 T7884 T7882 nsubj Mcm4,binds
R5274 T7885 T7884 punct /,Mcm4
R5275 T7886 T7884 nummod 6,Mcm4
R5276 T7887 T7884 punct /,Mcm4
R5277 T7888 T7884 nummod 7,Mcm4
R5278 T7889 T7882 prep to,binds
R5279 T7890 T7891 amod single,stranded
R5280 T7891 T7893 amod stranded,tails
R5281 T7892 T7891 punct -,stranded
R5282 T7893 T7889 pobj tails,to
R5283 T7894 T7853 punct .,bound
R5284 T7896 T7897 advmod However,displaced
R5285 T7898 T7897 punct ", ",displaced
R5286 T7899 T7897 prep in,displaced
R5287 T7900 T7901 compound DNA,helicase
R5288 T7901 T7902 compound helicase,assays
R5289 T7902 T7899 pobj assays,in
R5290 T7903 T7897 punct ", ",displaced
R5291 T7904 T7905 compound T,fork
R5292 T7905 T7897 nsubjpass fork,displaced
R5293 T7906 T7905 punct -,fork
R5294 T7907 T7905 punct /,fork
R5295 T7908 T7909 punct (,G
R5296 T7909 T7905 appos G,fork
R5297 T7910 T7909 nmod C,G
R5298 T7911 T7909 punct :,G
R5299 T7912 T7909 punct ),G
R5300 T7913 T7909 nummod 49,G
R5301 T7914 T7897 auxpass was,displaced
R5302 T7915 T7897 advmod hardly,displaced
R5303 T7916 T7897 agent by,displaced
R5304 T7917 T7916 pobj Mcm4,by
R5305 T7918 T7917 punct /,Mcm4
R5306 T7919 T7917 nummod 6,Mcm4
R5307 T7920 T7917 punct /,Mcm4
R5308 T7921 T7917 nummod 7,Mcm4
R5309 T7922 T7897 punct ", ",displaced
R5310 T7923 T7897 cc but,displaced
R5311 T7924 T7925 auxpass was,displaced
R5312 T7925 T7897 conj displaced,displaced
R5313 T7926 T7925 advmod readily,displaced
R5314 T7927 T7925 agent by,displaced
R5315 T7928 T7929 compound SV40,helicase
R5316 T7929 T7927 pobj helicase,by
R5317 T7930 T7931 compound T,antigen
R5318 T7931 T7929 compound antigen,helicase
R5319 T7932 T7931 punct -,antigen
R5320 T7933 T7929 compound DNA,helicase
R5321 T7934 T7935 punct (,8B
R5322 T7935 T7925 parataxis 8B,displaced
R5323 T7936 T7935 compound Figure,8B
R5324 T7937 T7935 punct ),8B
R5325 T7938 T7897 punct .,displaced
R5326 T7940 T7941 advmod When,inserted
R5327 T7941 T7946 advcl inserted,increased
R5328 T7942 T7941 nsubjpass thymine,inserted
R5329 T7943 T7942 cc or,thymine
R5330 T7944 T7942 conj adenine,thymine
R5331 T7945 T7941 auxpass is,inserted
R5332 T7947 T7941 prep as,inserted
R5333 T7948 T7949 det every,nucleotide
R5334 T7949 T7947 pobj nucleotide,as
R5335 T7950 T7949 amod third,nucleotide
R5336 T7951 T7952 punct (,repeats
R5337 T7952 T7949 parataxis repeats,nucleotide
R5338 T7953 T7952 prep of,repeats
R5339 T7954 T7953 pobj CCT,of
R5340 T7955 T7954 cc or,CCT
R5341 T7956 T7954 conj CCA,CCT
R5342 T7957 T7952 punct ),repeats
R5343 T7958 T7946 punct ", ",increased
R5344 T7959 T7960 det the,extent
R5345 T7960 T7946 nsubj extent,increased
R5346 T7961 T7960 prep of,extent
R5347 T7962 T7961 pobj unwinding,of
R5348 T7963 T7964 punct (,GGA
R5349 T7964 T7946 dobj GGA,increased
R5350 T7965 T7966 nmod T,fork
R5351 T7966 T7964 nmod fork,GGA
R5352 T7967 T7966 punct -,fork
R5353 T7968 T7964 punct /,GGA
R5354 T7969 T7964 punct (,GGA
R5355 T7970 T7964 nmod CCT,GGA
R5356 T7971 T7964 punct :,GGA
R5357 T7972 T7964 punct ),GGA
R5358 T7973 T7964 nummod 16,GGA
R5359 T7974 T7964 cc and,GGA
R5360 T7975 T7976 nmod T,fork
R5361 T7976 T7978 nmod fork,GGT
R5362 T7977 T7976 punct -,fork
R5363 T7978 T7964 conj GGT,GGA
R5364 T7979 T7978 punct /,GGT
R5365 T7980 T7978 punct (,GGT
R5366 T7981 T7978 nmod CCA,GGT
R5367 T7982 T7978 punct :,GGT
R5368 T7983 T7978 punct ),GGT
R5369 T7984 T7978 nummod 16,GGT
R5370 T7985 T7946 punct ),increased
R5371 T7986 T7946 punct .,increased
R5372 T7988 T7989 det The,efficiency
R5373 T7989 T7990 nsubjpass efficiency,correlated
R5374 T7991 T7989 prep of,efficiency
R5375 T7992 T7991 pobj unwinding,of
R5376 T7993 T7990 auxpass is,correlated
R5377 T7994 T7990 advmod roughly,correlated
R5378 T7995 T7990 prep with,correlated
R5379 T7996 T7997 det the,content
R5380 T7997 T7995 pobj content,with
R5381 T7998 T7997 prep of,content
R5382 T7999 T8000 compound GC,pairs
R5383 T8000 T7998 pobj pairs,of
R5384 T8001 T8000 prep in,pairs
R5385 T8002 T8003 det the,segment
R5386 T8003 T8001 pobj segment,in
R5387 T8004 T8003 compound duplex,segment
R5388 T8005 T8006 punct [,GGTT
R5389 T8006 T8000 appos GGTT,pairs
R5390 T8007 T8008 nmod T,fork
R5391 T8008 T8006 nmod fork,GGTT
R5392 T8009 T8008 punct -,fork
R5393 T8010 T8006 punct /,GGTT
R5394 T8011 T8006 punct (,GGTT
R5395 T8012 T8006 nmod CCAA,GGTT
R5396 T8013 T8006 punct :,GGTT
R5397 T8014 T8006 punct ),GGTT
R5398 T8015 T8006 nummod 12,GGTT
R5399 T8016 T8006 punct ", ",GGTT
R5400 T8017 T8018 nmod T,fork
R5401 T8018 T8020 nmod fork,GGAA
R5402 T8019 T8018 punct -,fork
R5403 T8020 T8006 conj GGAA,GGTT
R5404 T8021 T8020 punct /,GGAA
R5405 T8022 T8020 punct (,GGAA
R5406 T8023 T8020 nmod CCTT,GGAA
R5407 T8024 T8020 punct :,GGAA
R5408 T8025 T8020 punct ),GGAA
R5409 T8026 T8020 nummod 12,GGAA
R5410 T8027 T8020 punct ", ",GGAA
R5411 T8028 T8029 nmod T,fork
R5412 T8029 T8031 nmod fork,GTTT
R5413 T8030 T8029 punct -,fork
R5414 T8031 T8020 conj GTTT,GGAA
R5415 T8032 T8031 punct /,GTTT
R5416 T8033 T8031 punct (,GTTT
R5417 T8034 T8031 nmod CAAA,GTTT
R5418 T8035 T8031 punct :,GTTT
R5419 T8036 T8031 punct ),GTTT
R5420 T8037 T8031 nummod 12,GTTT
R5421 T8038 T8031 cc and,GTTT
R5422 T8039 T8040 nmod T,fork
R5423 T8040 T8042 nmod fork,GAAA
R5424 T8041 T8040 punct -,fork
R5425 T8042 T8031 conj GAAA,GTTT
R5426 T8043 T8042 punct /,GAAA
R5427 T8044 T8042 punct (,GAAA
R5428 T8045 T8042 nmod CTTT,GAAA
R5429 T8046 T8042 punct :,GAAA
R5430 T8047 T8042 punct ),GAAA
R5431 T8048 T8042 nummod 12,GAAA
R5432 T8049 T8050 punct ;,8B
R5433 T8050 T7990 parataxis 8B,correlated
R5434 T8051 T8050 compound Figure,8B
R5435 T8052 T8050 punct ],8B
R5436 T8053 T7990 punct .,correlated
R5437 T8055 T8056 nsubj It,appears
R5438 T8057 T8058 mark that,unwound
R5439 T8058 T8056 ccomp unwound,appears
R5440 T8059 T8060 det the,segment
R5441 T8060 T8058 nsubjpass segment,unwound
R5442 T8061 T8060 compound duplex,segment
R5443 T8062 T8060 acl containing,segment
R5444 T8063 T8064 punct <,50
R5445 T8064 T8065 nummod 50,%
R5446 T8065 T8066 compound %,pairs
R5447 T8066 T8062 dobj pairs,containing
R5448 T8067 T8066 compound GC,pairs
R5449 T8068 T8058 auxpass is,unwound
R5450 T8069 T8058 advmod efficiently,unwound
R5451 T8070 T8071 punct (,8C
R5452 T8071 T8058 parataxis 8C,unwound
R5453 T8072 T8071 compound Figure,8C
R5454 T8073 T8071 punct ),8C
R5455 T8074 T8056 punct .,appears
R5456 T8076 T8077 nsubj We,examined
R5457 T8078 T8077 advmod next,examined
R5458 T8079 T8080 det the,effect
R5459 T8080 T8077 dobj effect,examined
R5460 T8081 T8080 prep of,effect
R5461 T8082 T8083 npadvmod GC,rich
R5462 T8083 T8085 amod rich,segments
R5463 T8084 T8083 punct -,rich
R5464 T8085 T8081 pobj segments,of
R5465 T8086 T8080 prep on,effect
R5466 T8087 T8088 det a,DNA
R5467 T8088 T8086 pobj DNA,on
R5468 T8089 T8088 amod partial,DNA
R5469 T8090 T8088 compound heteroduplex,DNA
R5470 T8091 T8088 prep on,DNA
R5471 T8092 T8093 det a,DNA
R5472 T8093 T8091 pobj DNA,on
R5473 T8094 T8095 amod single,stranded
R5474 T8095 T8093 amod stranded,DNA
R5475 T8096 T8095 punct -,stranded
R5476 T8097 T8093 amod circular,DNA
R5477 T8098 T8077 punct .,examined
R5478 T8100 T8101 nsubj We,constructed
R5479 T8102 T8101 aux have,constructed
R5480 T8103 T8104 nummod two,sets
R5481 T8104 T8101 dobj sets,constructed
R5482 T8105 T8104 prep of,sets
R5483 T8106 T8107 npadvmod 5,tailed
R5484 T8107 T8110 amod tailed,substrates
R5485 T8108 T8106 punct ′,5
R5486 T8109 T8107 punct -,tailed
R5487 T8110 T8105 pobj substrates,of
R5488 T8111 T8112 amod partial,heteroduplex
R5489 T8112 T8110 compound heteroduplex,substrates
R5490 T8113 T8110 compound DNA,substrates
R5491 T8114 T8110 acl containing,substrates
R5492 T8115 T8116 compound duplex,regions
R5493 T8116 T8114 dobj regions,containing
R5494 T8117 T8116 prep of,regions
R5495 T8118 T8119 amod variable,lengths
R5496 T8119 T8117 pobj lengths,of
R5497 T8120 T8104 punct ;,sets
R5498 T8121 T8104 appos one,sets
R5499 T8122 T8121 prep on,one
R5500 T8123 T8124 compound M13mp18,vector
R5501 T8124 T8122 pobj vector,on
R5502 T8125 T8121 cc and,one
R5503 T8126 T8127 det the,other
R5504 T8127 T8121 conj other,one
R5505 T8128 T8127 prep on,other
R5506 T8129 T8128 pobj M13mp19,on
R5507 T8130 T8121 acl containing,one
R5508 T8131 T8132 det a,segment
R5509 T8132 T8130 dobj segment,containing
R5510 T8133 T8134 nummod 66,nt
R5511 T8134 T8135 npadvmod nt,long
R5512 T8135 T8132 amod long,segment
R5513 T8136 T8137 npadvmod G,rich
R5514 T8137 T8132 amod rich,segment
R5515 T8138 T8137 punct -,rich
R5516 T8139 T8132 advmod downstream,segment
R5517 T8140 T8139 prep of,downstream
R5518 T8141 T8142 det the,oligonucleotide
R5519 T8142 T8140 pobj oligonucleotide,of
R5520 T8143 T8142 amod hybridizing,oligonucleotide
R5521 T8144 T8101 punct .,constructed
R5522 T8146 T8147 nsubj We,found
R5523 T8148 T8149 mark that,displace
R5524 T8149 T8147 ccomp displace,found
R5525 T8150 T8151 det the,helicase
R5526 T8151 T8149 nsubj helicase,displace
R5527 T8152 T8151 nmod Mcm4,helicase
R5528 T8153 T8152 punct /,Mcm4
R5529 T8154 T8152 nummod 6,Mcm4
R5530 T8155 T8152 punct /,Mcm4
R5531 T8156 T8152 nummod 7,Mcm4
R5532 T8157 T8149 aux can,displace
R5533 T8158 T8159 compound duplex,DNA
R5534 T8159 T8149 dobj DNA,displace
R5535 T8160 T8161 quantmod up,350
R5536 T8161 T8163 nummod 350,nt
R5537 T8162 T8161 quantmod to,350
R5538 T8163 T8164 npadvmod nt,long
R5539 T8164 T8159 amod long,DNA
R5540 T8165 T8149 prep on,displace
R5541 T8166 T8167 poss its,own
R5542 T8167 T8165 pobj own,on
R5543 T8168 T8149 prep on,displace
R5544 T8169 T8170 det the,substrate
R5545 T8170 T8168 pobj substrate,on
R5546 T8171 T8170 amod former,substrate
R5547 T8172 T8173 punct (,8D
R5548 T8173 T8149 parataxis 8D,displace
R5549 T8174 T8173 compound Figure,8D
R5550 T8175 T8173 punct ", ",8D
R5551 T8176 T8173 amod left,8D
R5552 T8177 T8173 punct ),8D
R5553 T8178 T8147 punct ", ",found
R5554 T8179 T8147 advcl consistent,found
R5555 T8180 T8179 prep with,consistent
R5556 T8181 T8182 amod previous,results
R5557 T8182 T8180 pobj results,with
R5558 T8183 T8184 punct (,13
R5559 T8184 T8182 parataxis 13,results
R5560 T8185 T8184 punct ),13
R5561 T8186 T8147 punct .,found
R5562 T8188 T8189 prep In,inhibited
R5563 T8190 T8188 pobj contrast,In
R5564 T8191 T8189 punct ", ",inhibited
R5565 T8192 T8189 nsubjpass displacement,inhibited
R5566 T8193 T8189 auxpass was,inhibited
R5567 T8194 T8189 prep over,inhibited
R5568 T8195 T8196 det the,segments
R5569 T8196 T8194 pobj segments,over
R5570 T8197 T8198 npadvmod GC,rich
R5571 T8198 T8196 amod rich,segments
R5572 T8199 T8198 punct -,rich
R5573 T8200 T8196 prep on,segments
R5574 T8201 T8202 det the,substrate
R5575 T8202 T8200 pobj substrate,on
R5576 T8203 T8202 amod latter,substrate
R5577 T8204 T8205 punct (,panel
R5578 T8205 T8196 parataxis panel,segments
R5579 T8206 T8207 compound Figure,8D
R5580 T8207 T8205 dep 8D,panel
R5581 T8208 T8205 punct ", ",panel
R5582 T8209 T8205 amod right,panel
R5583 T8210 T8205 punct ),panel
R5584 T8211 T8189 punct ", ",inhibited
R5585 T8212 T8213 mark although,observed
R5586 T8213 T8189 advcl observed,inhibited
R5587 T8214 T8213 nsubjpass displacement,observed
R5588 T8215 T8216 quantmod up,250
R5589 T8216 T8218 nummod 250,nt
R5590 T8217 T8216 quantmod to,250
R5591 T8218 T8214 appos nt,displacement
R5592 T8219 T8218 prep in,nt
R5593 T8220 T8219 pobj length,in
R5594 T8221 T8218 punct ", ",nt
R5595 T8222 T8223 dep albeit,at
R5596 T8223 T8218 prep at,nt
R5597 T8224 T8225 det a,level
R5598 T8225 T8223 pobj level,at
R5599 T8226 T8225 amod reduced,level
R5600 T8227 T8213 punct ", ",observed
R5601 T8228 T8213 auxpass was,observed
R5602 T8229 T8213 prep at,observed
R5603 T8230 T8231 det a,concentration
R5604 T8231 T8229 pobj concentration,at
R5605 T8232 T8231 amod high,concentration
R5606 T8233 T8231 prep of,concentration
R5607 T8234 T8235 nmod Mcm4,complex
R5608 T8235 T8233 pobj complex,of
R5609 T8236 T8234 punct /,Mcm4
R5610 T8237 T8234 nummod 6,Mcm4
R5611 T8238 T8234 punct /,Mcm4
R5612 T8239 T8234 nummod 7,Mcm4
R5613 T8240 T8189 punct .,inhibited
R5614 T8242 T8243 det This,result
R5615 T8243 T8244 nsubj result,is
R5616 T8245 T8244 acomp consistent,is
R5617 T8246 T8245 prep with,consistent
R5618 T8247 T8246 pobj that,with
R5619 T8248 T8247 prep on,that
R5620 T8249 T8250 compound Y,fork
R5621 T8250 T8248 pobj fork,on
R5622 T8251 T8250 punct -,fork
R5623 T8252 T8244 cc and,is
R5624 T8253 T8244 conj indicates,is
R5625 T8254 T8255 mark that,is
R5626 T8255 T8253 ccomp is,indicates
R5627 T8256 T8257 npadvmod GC,rich
R5628 T8257 T8259 amod rich,segment
R5629 T8258 T8257 punct -,rich
R5630 T8259 T8255 nsubj segment,is
R5631 T8260 T8259 compound duplex,segment
R5632 T8261 T8255 acomp inhibitory,is
R5633 T8262 T8261 prep for,inhibitory
R5634 T8263 T8262 pobj unwinding,for
R5635 T8264 T8263 prep by,unwinding
R5636 T8265 T8266 det the,helicase
R5637 T8266 T8264 pobj helicase,by
R5638 T8267 T8266 compound Mcm,helicase
R5639 T8268 T8244 punct .,is
R5640 T8270 T8271 det These,results
R5641 T8271 T8272 nsubj results,indicate
R5642 T8273 T8272 aux may,indicate
R5643 T8274 T8275 mark that,facilitate
R5644 T8275 T8272 ccomp facilitate,indicate
R5645 T8276 T8277 det the,presence
R5646 T8277 T8275 nsubj presence,facilitate
R5647 T8278 T8277 prep of,presence
R5648 T8279 T8280 compound thymine,adenine
R5649 T8280 T8282 compound adenine,pairs
R5650 T8281 T8280 punct –,adenine
R5651 T8282 T8278 pobj pairs,of
R5652 T8283 T8282 prep with,pairs
R5653 T8284 T8285 det a,frequency
R5654 T8285 T8283 pobj frequency,with
R5655 T8286 T8285 amod certain,frequency
R5656 T8287 T8277 prep in,presence
R5657 T8288 T8289 det the,region
R5658 T8289 T8287 pobj region,in
R5659 T8290 T8289 compound duplex,region
R5660 T8291 T8275 aux may,facilitate
R5661 T8292 T8293 amod continuous,unwinding
R5662 T8293 T8275 dobj unwinding,facilitate
R5663 T8294 T8293 prep by,unwinding
R5664 T8295 T8294 pobj Mcm4,by
R5665 T8296 T8295 punct /,Mcm4
R5666 T8297 T8295 nummod 6,Mcm4
R5667 T8298 T8295 punct /,Mcm4
R5668 T8299 T8295 nummod 7,Mcm4
R5669 T8300 T8272 punct .,indicate
R5670 T8302 T8303 advmod Alternatively,is
R5671 T8304 T8303 punct ", ",is
R5672 T8305 T8303 nsubj Mcm4,is
R5673 T8306 T8305 punct /,Mcm4
R5674 T8307 T8305 nummod 6,Mcm4
R5675 T8308 T8305 punct /,Mcm4
R5676 T8309 T8305 nummod 7,Mcm4
R5677 T8310 T8303 neg not,is
R5678 T8311 T8303 acomp efficient,is
R5679 T8312 T8311 advmod enough,efficient
R5680 T8313 T8314 aux to,displace
R5681 T8314 T8311 xcomp displace,efficient
R5682 T8315 T8316 advmod thermodynamically,stable
R5683 T8316 T8317 amod stable,segment
R5684 T8317 T8314 dobj segment,displace
R5685 T8318 T8319 npadvmod GC,paired
R5686 T8319 T8317 amod paired,segment
R5687 T8320 T8319 punct -,paired
R5688 T8321 T8319 npadvmod base,paired
R5689 T8322 T8319 punct -,paired
R5690 T8323 T8303 punct .,is
R5691 T8325 T8326 prep In,designed
R5692 T8327 T8325 pobj order,In
R5693 T8328 T8329 aux to,distinguish
R5694 T8329 T8327 acl distinguish,order
R5695 T8330 T8331 det these,possibilities
R5696 T8331 T8329 dobj possibilities,distinguish
R5697 T8332 T8331 nummod two,possibilities
R5698 T8333 T8326 punct ", ",designed
R5699 T8334 T8326 nsubj we,designed
R5700 T8335 T8336 det a,substrate
R5701 T8336 T8326 dobj substrate,designed
R5702 T8337 T8336 amod new,substrate
R5703 T8338 T8339 compound T,fork
R5704 T8339 T8336 compound fork,substrate
R5705 T8340 T8339 punct -,fork
R5706 T8341 T8336 acl containing,substrate
R5707 T8342 T8343 nmod inosine,residue
R5708 T8343 T8341 dobj residue,containing
R5709 T8344 T8342 punct (,inosine
R5710 T8345 T8342 appos I,inosine
R5711 T8346 T8343 punct ),residue
R5712 T8347 T8348 advmod instead,of
R5713 T8348 T8343 prep of,residue
R5714 T8349 T8350 compound guanosine,residue
R5715 T8350 T8348 pobj residue,of
R5716 T8351 T8326 punct .,designed
R5717 T8353 T8354 det The,stability
R5718 T8354 T8356 nsubj stability,is
R5719 T8355 T8354 amod thermal,stability
R5720 T8357 T8354 prep of,stability
R5721 T8358 T8359 det the,pair
R5722 T8359 T8357 pobj pair,of
R5723 T8360 T8361 nmod I,C
R5724 T8361 T8359 compound C,pair
R5725 T8362 T8361 punct :,C
R5726 T8363 T8359 compound base,pair
R5727 T8364 T8356 acomp lower,is
R5728 T8365 T8364 prep than,lower
R5729 T8366 T8365 pobj that,than
R5730 T8367 T8366 prep of,that
R5731 T8368 T8369 det the,pair
R5732 T8369 T8367 pobj pair,of
R5733 T8370 T8371 nmod G,C
R5734 T8371 T8369 compound C,pair
R5735 T8372 T8371 punct :,C
R5736 T8373 T8369 compound base,pair
R5737 T8374 T8356 prep in,is
R5738 T8375 T8376 compound duplex,DNA
R5739 T8376 T8374 pobj DNA,in
R5740 T8377 T8356 prep due,is
R5741 T8378 T8377 pcomp to,due
R5742 T8379 T8377 pobj loss,due
R5743 T8380 T8379 prep of,loss
R5744 T8381 T8382 nummod one,bond
R5745 T8382 T8380 pobj bond,of
R5746 T8383 T8382 compound hydrogen,bond
R5747 T8384 T8356 cc and,is
R5748 T8385 T8356 conj is,is
R5749 T8386 T8387 advmod even,lower
R5750 T8387 T8385 acomp lower,is
R5751 T8388 T8387 prep than,lower
R5752 T8389 T8390 nmod A,T
R5753 T8390 T8392 compound T,pair
R5754 T8391 T8390 punct :,T
R5755 T8392 T8388 pobj pair,than
R5756 T8393 T8392 compound base,pair
R5757 T8394 T8395 punct (,31
R5758 T8395 T8392 parataxis 31,pair
R5759 T8396 T8395 punct ),31
R5760 T8397 T8356 punct .,is
R5761 T8399 T8400 nsubj We,constructed
R5762 T8401 T8402 det the,substrates
R5763 T8402 T8400 dobj substrates,constructed
R5764 T8403 T8404 npadvmod T,tailed
R5765 T8404 T8402 amod tailed,substrates
R5766 T8405 T8404 punct -,tailed
R5767 T8406 T8402 compound fork,substrates
R5768 T8407 T8402 acl containing,substrates
R5769 T8408 T8409 det the,segment
R5770 T8409 T8407 dobj segment,containing
R5771 T8410 T8411 punct (,CGG
R5772 T8411 T8409 nmod CGG,segment
R5773 T8412 T8411 nmod GCC,CGG
R5774 T8413 T8411 punct :,CGG
R5775 T8414 T8411 punct ),CGG
R5776 T8415 T8411 nummod 10,CGG
R5777 T8416 T8411 punct ", ",CGG
R5778 T8417 T8418 punct (,CIG
R5779 T8418 T8411 conj CIG,CGG
R5780 T8419 T8418 nmod GCC,CIG
R5781 T8420 T8418 punct :,CIG
R5782 T8421 T8418 punct ),CIG
R5783 T8422 T8418 nummod 10,CIG
R5784 T8423 T8418 cc or,CIG
R5785 T8424 T8425 punct (,CTT
R5786 T8425 T8418 conj CTT,CIG
R5787 T8426 T8425 nmod GAA,CTT
R5788 T8427 T8425 punct :,CTT
R5789 T8428 T8425 punct ),CTT
R5790 T8429 T8425 nummod 10,CTT
R5791 T8430 T8409 compound duplex,segment
R5792 T8431 T8409 compound DNA,segment
R5793 T8432 T8433 punct (,8E
R5794 T8433 T8407 parataxis 8E,containing
R5795 T8434 T8433 compound Figure,8E
R5796 T8435 T8433 punct ),8E
R5797 T8436 T8400 punct .,constructed
R5798 T8438 T8439 nsubj Mcm4,displaced
R5799 T8440 T8438 punct /,Mcm4
R5800 T8441 T8438 nummod 6,Mcm4
R5801 T8442 T8438 punct /,Mcm4
R5802 T8443 T8438 nummod 7,Mcm4
R5803 T8444 T8439 advmod hardly,displaced
R5804 T8445 T8446 det the,duplex
R5805 T8446 T8439 dobj duplex,displaced
R5806 T8447 T8448 nummod 31,nt
R5807 T8448 T8449 npadvmod nt,long
R5808 T8449 T8446 amod long,duplex
R5809 T8450 T8446 prep of,duplex
R5810 T8451 T8452 nmod GCC,CGG
R5811 T8452 T8454 compound CGG,repeats
R5812 T8453 T8452 punct :,CGG
R5813 T8454 T8450 pobj repeats,of
R5814 T8455 T8439 punct ", ",displaced
R5815 T8456 T8457 mark whereas,displaced
R5816 T8457 T8439 advcl displaced,displaced
R5817 T8458 T8459 det the,CTT
R5818 T8459 T8457 nsubjpass CTT,displaced
R5819 T8460 T8461 nmod T,fork
R5820 T8461 T8459 nmod fork,CTT
R5821 T8462 T8461 punct -,fork
R5822 T8463 T8459 punct /,CTT
R5823 T8464 T8459 punct (,CTT
R5824 T8465 T8459 nmod GAA,CTT
R5825 T8466 T8459 punct :,CTT
R5826 T8467 T8459 punct ),CTT
R5827 T8468 T8459 nummod 10,CTT
R5828 T8469 T8457 auxpass was,displaced
R5829 T8470 T8457 advmod efficiently,displaced
R5830 T8471 T8457 punct ", ",displaced
R5831 T8472 T8473 mark as,described
R5832 T8473 T8457 advcl described,displaced
R5833 T8474 T8473 advmod above,described
R5834 T8475 T8439 punct .,displaced
R5835 T8477 T8478 nmod T,fork
R5836 T8478 T8480 nmod fork,CIG
R5837 T8479 T8478 punct -,fork
R5838 T8480 T8485 nsubjpass CIG,displaced
R5839 T8481 T8480 punct /,CIG
R5840 T8482 T8480 punct (,CIG
R5841 T8483 T8480 nmod GCC,CIG
R5842 T8484 T8480 punct :,CIG
R5843 T8486 T8480 punct ),CIG
R5844 T8487 T8480 nummod 10,CIG
R5845 T8488 T8485 auxpass was,displaced
R5846 T8489 T8485 prep with,displaced
R5847 T8490 T8489 pobj efficiency,with
R5848 T8491 T8492 advmod much,greater
R5849 T8492 T8490 amod greater,efficiency
R5850 T8493 T8492 prep than,greater
R5851 T8494 T8493 pobj that,than
R5852 T8495 T8494 prep of,that
R5853 T8496 T8497 nmod T,fork
R5854 T8497 T8499 nmod fork,CGG
R5855 T8498 T8497 punct -,fork
R5856 T8499 T8495 pobj CGG,of
R5857 T8500 T8497 punct /,fork
R5858 T8501 T8499 punct (,CGG
R5859 T8502 T8499 nmod GCC,CGG
R5860 T8503 T8499 punct :,CGG
R5861 T8504 T8499 punct ),CGG
R5862 T8505 T8499 nummod 10,CGG
R5863 T8506 T8485 punct ", ",displaced
R5864 T8507 T8485 advcl indicating,displaced
R5865 T8508 T8509 mark that,is
R5866 T8509 T8507 ccomp is,indicating
R5867 T8510 T8511 det the,thermostability
R5868 T8511 T8509 nsubj thermostability,is
R5869 T8512 T8511 prep of,thermostability
R5870 T8513 T8514 det the,segment
R5871 T8514 T8512 pobj segment,of
R5872 T8515 T8514 compound duplex,segment
R5873 T8516 T8511 punct ", ",thermostability
R5874 T8517 T8511 cc not,thermostability
R5875 T8518 T8519 det the,lack
R5876 T8519 T8511 conj lack,thermostability
R5877 T8520 T8519 prep of,lack
R5878 T8521 T8522 compound AT,pair
R5879 T8522 T8520 pobj pair,of
R5880 T8523 T8522 compound base,pair
R5881 T8524 T8509 punct ", ",is
R5882 T8525 T8509 acomp responsible,is
R5883 T8526 T8525 prep for,responsible
R5884 T8527 T8526 pobj inability,for
R5885 T8528 T8527 prep of,inability
R5886 T8529 T8528 pobj Mcm4,of
R5887 T8530 T8529 punct /,Mcm4
R5888 T8531 T8529 nummod 6,Mcm4
R5889 T8532 T8529 punct /,Mcm4
R5890 T8533 T8529 nummod 7,Mcm4
R5891 T8534 T8535 aux to,displace
R5892 T8535 T8527 acl displace,inability
R5893 T8536 T8537 det the,duplex
R5894 T8537 T8535 dobj duplex,displace
R5895 T8538 T8485 punct .,displaced