Id |
Subject |
Object |
Predicate |
Lexical cue |
T5933 |
0-1 |
CD |
denotes |
3 |
T5935 |
1-2 |
SYM |
denotes |
′ |
T5936 |
2-3 |
HYPH |
denotes |
- |
T5934 |
3-12 |
NN |
denotes |
extension |
T5938 |
13-17 |
IN |
denotes |
with |
T5939 |
18-22 |
NN |
denotes |
poly |
T5941 |
22-23 |
-LRB- |
denotes |
( |
T5940 |
23-25 |
NN |
denotes |
dT |
T5943 |
25-26 |
-RRB- |
denotes |
) |
T5942 |
27-31 |
NN |
denotes |
tail |
T5944 |
32-34 |
VBZ |
denotes |
is |
T5937 |
35-44 |
VBN |
denotes |
displaced |
T5945 |
45-47 |
IN |
denotes |
by |
T5946 |
48-52 |
NN |
denotes |
Mcm4 |
T5947 |
52-53 |
HYPH |
denotes |
/ |
T5948 |
53-54 |
CD |
denotes |
6 |
T5949 |
54-55 |
HYPH |
denotes |
/ |
T5950 |
55-56 |
CD |
denotes |
7 |
T5951 |
56-209 |
sentence |
denotes |
It has been reported that the 3′-extension can be unwound by the archaeal Mcm homohexamer but not by fission and budding yeast Mcm complexes (14,26,27). |
T5952 |
57-59 |
PRP |
denotes |
It |
T5954 |
60-63 |
VBZ |
denotes |
has |
T5955 |
64-68 |
VBN |
denotes |
been |
T5953 |
69-77 |
VBN |
denotes |
reported |
T5956 |
78-82 |
IN |
denotes |
that |
T5958 |
83-86 |
DT |
denotes |
the |
T5960 |
87-88 |
CD |
denotes |
3 |
T5961 |
88-89 |
SYM |
denotes |
′ |
T5962 |
89-90 |
HYPH |
denotes |
- |
T5959 |
90-99 |
NN |
denotes |
extension |
T5963 |
100-103 |
MD |
denotes |
can |
T5964 |
104-106 |
VB |
denotes |
be |
T5957 |
107-114 |
JJ |
denotes |
unwound |
T5965 |
115-117 |
IN |
denotes |
by |
T5966 |
118-121 |
DT |
denotes |
the |
T5968 |
122-130 |
JJ |
denotes |
archaeal |
T5969 |
131-134 |
NN |
denotes |
Mcm |
T5967 |
135-146 |
NN |
denotes |
homohexamer |
T5970 |
147-150 |
CC |
denotes |
but |
T5971 |
151-154 |
RB |
denotes |
not |
T5972 |
155-157 |
IN |
denotes |
by |
T5973 |
158-165 |
NN |
denotes |
fission |
T5975 |
166-169 |
CC |
denotes |
and |
T5976 |
170-177 |
NN |
denotes |
budding |
T5977 |
178-183 |
NN |
denotes |
yeast |
T5978 |
184-187 |
NN |
denotes |
Mcm |
T5974 |
188-197 |
NNS |
denotes |
complexes |
T5979 |
198-199 |
-LRB- |
denotes |
( |
T5981 |
199-201 |
CD |
denotes |
14 |
T5982 |
201-202 |
, |
denotes |
, |
T5983 |
202-204 |
CD |
denotes |
26 |
T5984 |
204-205 |
, |
denotes |
, |
T5980 |
205-207 |
CD |
denotes |
27 |
T5985 |
207-208 |
-RRB- |
denotes |
) |
T5986 |
208-209 |
. |
denotes |
. |
T5987 |
209-389 |
sentence |
denotes |
We have constructed 3′-extension substrates containing poly(dT), poly(dA), poly(dC) and poly(dG) 3′-tails in order to examine whether mouse Mcm4/6/7 can displace these substrates. |
T5988 |
210-212 |
PRP |
denotes |
We |
T5990 |
213-217 |
VBP |
denotes |
have |
T5989 |
218-229 |
VBN |
denotes |
constructed |
T5991 |
230-231 |
CD |
denotes |
3 |
T5993 |
231-232 |
SYM |
denotes |
′ |
T5994 |
232-233 |
HYPH |
denotes |
- |
T5992 |
233-242 |
NN |
denotes |
extension |
T5995 |
243-253 |
NNS |
denotes |
substrates |
T5996 |
254-264 |
VBG |
denotes |
containing |
T5997 |
265-269 |
NN |
denotes |
poly |
T5999 |
269-270 |
-LRB- |
denotes |
( |
T5998 |
270-272 |
NN |
denotes |
dT |
T6001 |
272-273 |
-RRB- |
denotes |
) |
T6002 |
273-275 |
, |
denotes |
, |
T6003 |
275-279 |
NN |
denotes |
poly |
T6005 |
279-280 |
-LRB- |
denotes |
( |
T6004 |
280-282 |
NN |
denotes |
dA |
T6006 |
282-283 |
-RRB- |
denotes |
) |
T6007 |
283-285 |
, |
denotes |
, |
T6008 |
285-289 |
NN |
denotes |
poly |
T6010 |
289-290 |
-LRB- |
denotes |
( |
T6009 |
290-292 |
NN |
denotes |
dC |
T6011 |
292-293 |
-RRB- |
denotes |
) |
T6012 |
294-297 |
CC |
denotes |
and |
T6013 |
298-302 |
NN |
denotes |
poly |
T6015 |
302-303 |
-LRB- |
denotes |
( |
T6014 |
303-305 |
NN |
denotes |
dG |
T6016 |
305-306 |
-RRB- |
denotes |
) |
T6017 |
307-308 |
CD |
denotes |
3 |
T6018 |
308-309 |
SYM |
denotes |
′ |
T6019 |
309-310 |
HYPH |
denotes |
- |
T6000 |
310-315 |
NNS |
denotes |
tails |
T6020 |
316-318 |
IN |
denotes |
in |
T6021 |
319-324 |
NN |
denotes |
order |
T6022 |
325-327 |
TO |
denotes |
to |
T6023 |
328-335 |
VB |
denotes |
examine |
T6024 |
336-343 |
IN |
denotes |
whether |
T6026 |
344-349 |
NN |
denotes |
mouse |
T6027 |
350-354 |
NN |
denotes |
Mcm4 |
T6028 |
354-355 |
HYPH |
denotes |
/ |
T6029 |
355-356 |
CD |
denotes |
6 |
T6030 |
356-357 |
HYPH |
denotes |
/ |
T6031 |
357-358 |
CD |
denotes |
7 |
T6032 |
359-362 |
MD |
denotes |
can |
T6025 |
363-371 |
VB |
denotes |
displace |
T6033 |
372-377 |
DT |
denotes |
these |
T6034 |
378-388 |
NNS |
denotes |
substrates |
T6035 |
388-389 |
. |
denotes |
. |
T6036 |
389-540 |
sentence |
denotes |
We first found that Mcm4/6/7 binds to dT-, dC- and dG-3′-extension with similar affinity, and to dA-3′-extension with much lower affinity (Figure 5A). |
T6037 |
390-392 |
PRP |
denotes |
We |
T6039 |
393-398 |
RB |
denotes |
first |
T6038 |
399-404 |
VBD |
denotes |
found |
T6040 |
405-409 |
IN |
denotes |
that |
T6042 |
410-414 |
NN |
denotes |
Mcm4 |
T6043 |
414-415 |
HYPH |
denotes |
/ |
T6044 |
415-416 |
CD |
denotes |
6 |
T6045 |
416-417 |
HYPH |
denotes |
/ |
T6046 |
417-418 |
CD |
denotes |
7 |
T6041 |
419-424 |
VBZ |
denotes |
binds |
T6047 |
425-427 |
IN |
denotes |
to |
T6048 |
428-430 |
NN |
denotes |
dT |
T6050 |
430-431 |
HYPH |
denotes |
- |
T6051 |
431-433 |
, |
denotes |
, |
T6052 |
433-435 |
NN |
denotes |
dC |
T6053 |
435-436 |
HYPH |
denotes |
- |
T6054 |
437-440 |
CC |
denotes |
and |
T6055 |
441-443 |
NN |
denotes |
dG |
T6056 |
443-444 |
HYPH |
denotes |
- |
T6057 |
444-445 |
CD |
denotes |
3 |
T6058 |
445-446 |
SYM |
denotes |
′ |
T6059 |
446-447 |
HYPH |
denotes |
- |
T6049 |
447-456 |
NN |
denotes |
extension |
T6060 |
457-461 |
IN |
denotes |
with |
T6061 |
462-469 |
JJ |
denotes |
similar |
T6062 |
470-478 |
NN |
denotes |
affinity |
T6063 |
478-480 |
, |
denotes |
, |
T6064 |
480-483 |
CC |
denotes |
and |
T6065 |
484-486 |
IN |
denotes |
to |
T6066 |
487-489 |
NN |
denotes |
dA |
T6068 |
489-490 |
HYPH |
denotes |
- |
T6069 |
490-491 |
CD |
denotes |
3 |
T6070 |
491-492 |
SYM |
denotes |
′ |
T6071 |
492-493 |
HYPH |
denotes |
- |
T6067 |
493-502 |
NN |
denotes |
extension |
T6072 |
503-507 |
IN |
denotes |
with |
T6073 |
508-512 |
RB |
denotes |
much |
T6074 |
513-518 |
JJR |
denotes |
lower |
T6075 |
519-527 |
NN |
denotes |
affinity |
T6076 |
528-529 |
-LRB- |
denotes |
( |
T6078 |
529-535 |
NN |
denotes |
Figure |
T6077 |
536-538 |
NN |
denotes |
5A |
T6079 |
538-539 |
-RRB- |
denotes |
) |
T6080 |
539-540 |
. |
denotes |
. |
T6081 |
540-671 |
sentence |
denotes |
This is consistent with the previous results that Mcm4/6/7 binds to 50mer oligo-dT, -dC and -dG, but not to a 50mer oligo-dA (13). |
T6082 |
541-545 |
DT |
denotes |
This |
T6083 |
546-548 |
VBZ |
denotes |
is |
T6084 |
549-559 |
JJ |
denotes |
consistent |
T6085 |
560-564 |
IN |
denotes |
with |
T6086 |
565-568 |
DT |
denotes |
the |
T6088 |
569-577 |
JJ |
denotes |
previous |
T6087 |
578-585 |
NNS |
denotes |
results |
T6089 |
586-590 |
IN |
denotes |
that |
T6091 |
591-595 |
NN |
denotes |
Mcm4 |
T6092 |
595-596 |
HYPH |
denotes |
/ |
T6093 |
596-597 |
CD |
denotes |
6 |
T6094 |
597-598 |
HYPH |
denotes |
/ |
T6095 |
598-599 |
CD |
denotes |
7 |
T6090 |
600-605 |
VBZ |
denotes |
binds |
T6096 |
606-608 |
IN |
denotes |
to |
T6097 |
609-614 |
NN |
denotes |
50mer |
T6099 |
615-620 |
NN |
denotes |
oligo |
T6100 |
620-621 |
HYPH |
denotes |
- |
T6098 |
621-623 |
NN |
denotes |
dT |
T6101 |
623-625 |
, |
denotes |
, |
T6102 |
625-626 |
HYPH |
denotes |
- |
T6103 |
626-628 |
NN |
denotes |
dC |
T6104 |
629-632 |
CC |
denotes |
and |
T6105 |
633-634 |
HYPH |
denotes |
- |
T6106 |
634-636 |
NN |
denotes |
dG |
T6107 |
636-638 |
, |
denotes |
, |
T6108 |
638-641 |
CC |
denotes |
but |
T6109 |
642-645 |
RB |
denotes |
not |
T6110 |
646-648 |
IN |
denotes |
to |
T6111 |
649-650 |
DT |
denotes |
a |
T6113 |
651-656 |
NN |
denotes |
50mer |
T6114 |
657-662 |
NN |
denotes |
oligo |
T6115 |
662-663 |
HYPH |
denotes |
- |
T6112 |
663-665 |
NN |
denotes |
dA |
T6116 |
666-667 |
-LRB- |
denotes |
( |
T6117 |
667-669 |
CD |
denotes |
13 |
T6118 |
669-670 |
-RRB- |
denotes |
) |
T6119 |
670-671 |
. |
denotes |
. |
T6120 |
671-839 |
sentence |
denotes |
We found that dT-tailed 3′-extension was displaced efficiently by Mcm 4/6/7 while almost no or very little activity was detected on other substrates (Figure 5B and C). |
T6121 |
672-674 |
PRP |
denotes |
We |
T6122 |
675-680 |
VBD |
denotes |
found |
T6123 |
681-685 |
IN |
denotes |
that |
T6125 |
686-688 |
NN |
denotes |
dT |
T6127 |
688-689 |
HYPH |
denotes |
- |
T6126 |
689-695 |
VBN |
denotes |
tailed |
T6129 |
696-697 |
CD |
denotes |
3 |
T6130 |
697-698 |
SYM |
denotes |
′ |
T6131 |
698-699 |
HYPH |
denotes |
- |
T6128 |
699-708 |
NN |
denotes |
extension |
T6132 |
709-712 |
VBD |
denotes |
was |
T6124 |
713-722 |
VBN |
denotes |
displaced |
T6133 |
723-734 |
RB |
denotes |
efficiently |
T6134 |
735-737 |
IN |
denotes |
by |
T6135 |
738-741 |
NN |
denotes |
Mcm |
T6136 |
742-743 |
CD |
denotes |
4 |
T6137 |
743-744 |
HYPH |
denotes |
/ |
T6138 |
744-745 |
CD |
denotes |
6 |
T6139 |
745-746 |
HYPH |
denotes |
/ |
T6140 |
746-747 |
CD |
denotes |
7 |
T6141 |
748-753 |
IN |
denotes |
while |
T6143 |
754-760 |
RB |
denotes |
almost |
T6144 |
761-763 |
DT |
denotes |
no |
T6145 |
764-766 |
CC |
denotes |
or |
T6146 |
767-771 |
RB |
denotes |
very |
T6147 |
772-778 |
JJ |
denotes |
little |
T6148 |
779-787 |
NN |
denotes |
activity |
T6149 |
788-791 |
VBD |
denotes |
was |
T6142 |
792-800 |
VBN |
denotes |
detected |
T6150 |
801-803 |
IN |
denotes |
on |
T6151 |
804-809 |
JJ |
denotes |
other |
T6152 |
810-820 |
NNS |
denotes |
substrates |
T6153 |
821-822 |
-LRB- |
denotes |
( |
T6155 |
822-828 |
NN |
denotes |
Figure |
T6154 |
829-831 |
NN |
denotes |
5B |
T6156 |
832-835 |
CC |
denotes |
and |
T6157 |
836-837 |
NN |
denotes |
C |
T6158 |
837-838 |
-RRB- |
denotes |
) |
T6159 |
838-839 |
. |
denotes |
. |
T6160 |
839-953 |
sentence |
denotes |
This is in sharp contrast to yeast Mcm4/6/7 helicases which are not capable of unwinding 3′-extension substrates. |
T6161 |
840-844 |
DT |
denotes |
This |
T6162 |
845-847 |
VBZ |
denotes |
is |
T6163 |
848-850 |
IN |
denotes |
in |
T6164 |
851-856 |
JJ |
denotes |
sharp |
T6165 |
857-865 |
NN |
denotes |
contrast |
T6166 |
866-868 |
IN |
denotes |
to |
T6167 |
869-874 |
NN |
denotes |
yeast |
T6169 |
875-879 |
NN |
denotes |
Mcm4 |
T6170 |
879-880 |
HYPH |
denotes |
/ |
T6171 |
880-881 |
CD |
denotes |
6 |
T6172 |
881-882 |
HYPH |
denotes |
/ |
T6173 |
882-883 |
CD |
denotes |
7 |
T6168 |
884-893 |
NNS |
denotes |
helicases |
T6174 |
894-899 |
WDT |
denotes |
which |
T6175 |
900-903 |
VBP |
denotes |
are |
T6176 |
904-907 |
RB |
denotes |
not |
T6177 |
908-915 |
JJ |
denotes |
capable |
T6178 |
916-918 |
IN |
denotes |
of |
T6179 |
919-928 |
VBG |
denotes |
unwinding |
T6180 |
929-930 |
CD |
denotes |
3 |
T6182 |
930-931 |
SYM |
denotes |
′ |
T6183 |
931-932 |
HYPH |
denotes |
- |
T6181 |
932-941 |
NN |
denotes |
extension |
T6184 |
942-952 |
NNS |
denotes |
substrates |
T6185 |
952-953 |
. |
denotes |
. |
T6186 |
953-1059 |
sentence |
denotes |
Furthermore, dT-tailed 5′-extension, to which Mcm4/6/7 could bind, was not displaced by the Mcm helicase. |
T6187 |
954-965 |
RB |
denotes |
Furthermore |
T6189 |
965-967 |
, |
denotes |
, |
T6190 |
967-969 |
NN |
denotes |
dT |
T6192 |
969-970 |
HYPH |
denotes |
- |
T6191 |
970-976 |
VBN |
denotes |
tailed |
T6194 |
977-978 |
CD |
denotes |
5 |
T6195 |
978-979 |
SYM |
denotes |
′ |
T6196 |
979-980 |
HYPH |
denotes |
- |
T6193 |
980-989 |
NN |
denotes |
extension |
T6197 |
989-991 |
, |
denotes |
, |
T6198 |
991-993 |
IN |
denotes |
to |
T6200 |
994-999 |
WDT |
denotes |
which |
T6201 |
1000-1004 |
NN |
denotes |
Mcm4 |
T6202 |
1004-1005 |
HYPH |
denotes |
/ |
T6203 |
1005-1006 |
CD |
denotes |
6 |
T6204 |
1006-1007 |
HYPH |
denotes |
/ |
T6205 |
1007-1008 |
CD |
denotes |
7 |
T6206 |
1009-1014 |
MD |
denotes |
could |
T6199 |
1015-1019 |
VB |
denotes |
bind |
T6207 |
1019-1021 |
, |
denotes |
, |
T6208 |
1021-1024 |
VBD |
denotes |
was |
T6209 |
1025-1028 |
RB |
denotes |
not |
T6188 |
1029-1038 |
VBN |
denotes |
displaced |
T6210 |
1039-1041 |
IN |
denotes |
by |
T6211 |
1042-1045 |
DT |
denotes |
the |
T6213 |
1046-1049 |
NN |
denotes |
Mcm |
T6212 |
1050-1058 |
NN |
denotes |
helicase |
T6214 |
1058-1059 |
. |
denotes |
. |
T6215 |
1059-1250 |
sentence |
denotes |
The addition of the 5′-tail to dT-tailed 3′-extension further stimulated the displacement (Figure 5D), confirming the previous report that the fork structures facilitate the helicase action. |
T6216 |
1060-1063 |
DT |
denotes |
The |
T6217 |
1064-1072 |
NN |
denotes |
addition |
T6219 |
1073-1075 |
IN |
denotes |
of |
T6220 |
1076-1079 |
DT |
denotes |
the |
T6222 |
1080-1081 |
CD |
denotes |
5 |
T6223 |
1081-1082 |
SYM |
denotes |
′ |
T6224 |
1082-1083 |
HYPH |
denotes |
- |
T6221 |
1083-1087 |
NN |
denotes |
tail |
T6225 |
1088-1090 |
IN |
denotes |
to |
T6226 |
1091-1093 |
NN |
denotes |
dT |
T6228 |
1093-1094 |
HYPH |
denotes |
- |
T6227 |
1094-1100 |
VBN |
denotes |
tailed |
T6230 |
1101-1102 |
CD |
denotes |
3 |
T6231 |
1102-1103 |
SYM |
denotes |
′ |
T6232 |
1103-1104 |
HYPH |
denotes |
- |
T6229 |
1104-1113 |
NN |
denotes |
extension |
T6233 |
1114-1121 |
RB |
denotes |
further |
T6218 |
1122-1132 |
VBD |
denotes |
stimulated |
T6234 |
1133-1136 |
DT |
denotes |
the |
T6235 |
1137-1149 |
NN |
denotes |
displacement |
T6236 |
1150-1151 |
-LRB- |
denotes |
( |
T6238 |
1151-1157 |
NN |
denotes |
Figure |
T6237 |
1158-1160 |
NN |
denotes |
5D |
T6239 |
1160-1161 |
-RRB- |
denotes |
) |
T6240 |
1161-1163 |
, |
denotes |
, |
T6241 |
1163-1173 |
VBG |
denotes |
confirming |
T6242 |
1174-1177 |
DT |
denotes |
the |
T6244 |
1178-1186 |
JJ |
denotes |
previous |
T6243 |
1187-1193 |
NN |
denotes |
report |
T6245 |
1194-1198 |
IN |
denotes |
that |
T6247 |
1199-1202 |
DT |
denotes |
the |
T6249 |
1203-1207 |
NN |
denotes |
fork |
T6248 |
1208-1218 |
NNS |
denotes |
structures |
T6246 |
1219-1229 |
VBP |
denotes |
facilitate |
T6250 |
1230-1233 |
DT |
denotes |
the |
T6252 |
1234-1242 |
NN |
denotes |
helicase |
T6251 |
1243-1249 |
NN |
denotes |
action |
T6253 |
1249-1250 |
. |
denotes |
. |
T6254 |
1250-1414 |
sentence |
denotes |
Thus, these results further strengthen our conclusion that the mammalian Mcm helicase is generally activated by the thymine-stretch on the 3′-single-stranded tail. |
T6255 |
1251-1255 |
RB |
denotes |
Thus |
T6257 |
1255-1257 |
, |
denotes |
, |
T6258 |
1257-1262 |
DT |
denotes |
these |
T6259 |
1263-1270 |
NNS |
denotes |
results |
T6260 |
1271-1278 |
RB |
denotes |
further |
T6256 |
1279-1289 |
VB |
denotes |
strengthen |
T6261 |
1290-1293 |
PRP$ |
denotes |
our |
T6262 |
1294-1304 |
NN |
denotes |
conclusion |
T6263 |
1305-1309 |
IN |
denotes |
that |
T6265 |
1310-1313 |
DT |
denotes |
the |
T6267 |
1314-1323 |
JJ |
denotes |
mammalian |
T6268 |
1324-1327 |
NN |
denotes |
Mcm |
T6266 |
1328-1336 |
NN |
denotes |
helicase |
T6269 |
1337-1339 |
VBZ |
denotes |
is |
T6270 |
1340-1349 |
RB |
denotes |
generally |
T6264 |
1350-1359 |
VBN |
denotes |
activated |
T6271 |
1360-1362 |
IN |
denotes |
by |
T6272 |
1363-1366 |
DT |
denotes |
the |
T6274 |
1367-1374 |
NN |
denotes |
thymine |
T6275 |
1374-1375 |
HYPH |
denotes |
- |
T6273 |
1375-1382 |
NN |
denotes |
stretch |
T6276 |
1383-1385 |
IN |
denotes |
on |
T6277 |
1386-1389 |
DT |
denotes |
the |
T6279 |
1390-1391 |
CD |
denotes |
3 |
T6280 |
1391-1392 |
SYM |
denotes |
′ |
T6281 |
1392-1393 |
HYPH |
denotes |
- |
T6282 |
1393-1399 |
JJ |
denotes |
single |
T6284 |
1399-1400 |
HYPH |
denotes |
- |
T6283 |
1400-1408 |
VBN |
denotes |
stranded |
T6278 |
1409-1413 |
NN |
denotes |
tail |
T6285 |
1413-1414 |
. |
denotes |
. |
R3967 |
T5933 |
T5934 |
nummod |
3,extension |
R3968 |
T5934 |
T5937 |
nsubjpass |
extension,displaced |
R3969 |
T5935 |
T5933 |
punct |
′,3 |
R3970 |
T5936 |
T5934 |
punct |
-,extension |
R3971 |
T5938 |
T5934 |
prep |
with,extension |
R3972 |
T5939 |
T5940 |
nmod |
poly,dT |
R3973 |
T5940 |
T5942 |
nmod |
dT,tail |
R3974 |
T5941 |
T5940 |
punct |
(,dT |
R3975 |
T5942 |
T5938 |
pobj |
tail,with |
R3976 |
T5943 |
T5942 |
punct |
),tail |
R3977 |
T5944 |
T5937 |
auxpass |
is,displaced |
R3978 |
T5945 |
T5937 |
agent |
by,displaced |
R3979 |
T5946 |
T5945 |
pobj |
Mcm4,by |
R3980 |
T5947 |
T5946 |
punct |
/,Mcm4 |
R3981 |
T5948 |
T5946 |
nummod |
6,Mcm4 |
R3982 |
T5949 |
T5946 |
punct |
/,Mcm4 |
R3983 |
T5950 |
T5946 |
nummod |
7,Mcm4 |
R3984 |
T5952 |
T5953 |
nsubjpass |
It,reported |
R3985 |
T5954 |
T5953 |
aux |
has,reported |
R3986 |
T5955 |
T5953 |
auxpass |
been,reported |
R3987 |
T5956 |
T5957 |
mark |
that,unwound |
R3988 |
T5957 |
T5953 |
ccomp |
unwound,reported |
R3989 |
T5958 |
T5959 |
det |
the,extension |
R3990 |
T5959 |
T5957 |
nsubj |
extension,unwound |
R3991 |
T5960 |
T5959 |
nummod |
3,extension |
R3992 |
T5961 |
T5960 |
punct |
′,3 |
R3993 |
T5962 |
T5959 |
punct |
-,extension |
R3994 |
T5963 |
T5957 |
aux |
can,unwound |
R3995 |
T5964 |
T5957 |
aux |
be,unwound |
R3996 |
T5965 |
T5957 |
agent |
by,unwound |
R3997 |
T5966 |
T5967 |
det |
the,homohexamer |
R3998 |
T5967 |
T5965 |
pobj |
homohexamer,by |
R3999 |
T5968 |
T5967 |
amod |
archaeal,homohexamer |
R4000 |
T5969 |
T5967 |
compound |
Mcm,homohexamer |
R4001 |
T5970 |
T5957 |
cc |
but,unwound |
R4002 |
T5971 |
T5957 |
conj |
not,unwound |
R4003 |
T5972 |
T5971 |
agent |
by,not |
R4004 |
T5973 |
T5974 |
nmod |
fission,complexes |
R4005 |
T5974 |
T5972 |
pobj |
complexes,by |
R4006 |
T5975 |
T5973 |
cc |
and,fission |
R4007 |
T5976 |
T5973 |
conj |
budding,fission |
R4008 |
T5977 |
T5974 |
compound |
yeast,complexes |
R4009 |
T5978 |
T5974 |
compound |
Mcm,complexes |
R4010 |
T5979 |
T5980 |
punct |
(,27 |
R4011 |
T5980 |
T5953 |
parataxis |
27,reported |
R4012 |
T5981 |
T5980 |
nummod |
14,27 |
R4013 |
T5982 |
T5980 |
punct |
",",27 |
R4014 |
T5983 |
T5980 |
nummod |
26,27 |
R4015 |
T5984 |
T5980 |
punct |
",",27 |
R4016 |
T5985 |
T5980 |
punct |
),27 |
R4017 |
T5986 |
T5953 |
punct |
.,reported |
R4018 |
T5988 |
T5989 |
nsubj |
We,constructed |
R4019 |
T5990 |
T5989 |
aux |
have,constructed |
R4020 |
T5991 |
T5992 |
nummod |
3,extension |
R4021 |
T5992 |
T5995 |
compound |
extension,substrates |
R4022 |
T5993 |
T5991 |
punct |
′,3 |
R4023 |
T5994 |
T5992 |
punct |
-,extension |
R4024 |
T5995 |
T5989 |
dobj |
substrates,constructed |
R4025 |
T5996 |
T5995 |
acl |
containing,substrates |
R4026 |
T5997 |
T5998 |
nmod |
poly,dT |
R4027 |
T5998 |
T6000 |
nmod |
dT,tails |
R4028 |
T5999 |
T5998 |
punct |
(,dT |
R4029 |
T6000 |
T5996 |
dobj |
tails,containing |
R4030 |
T6001 |
T5998 |
punct |
),dT |
R4031 |
T6002 |
T5998 |
punct |
", ",dT |
R4032 |
T6003 |
T6004 |
nmod |
poly,dA |
R4033 |
T6004 |
T5998 |
conj |
dA,dT |
R4034 |
T6005 |
T6004 |
punct |
(,dA |
R4035 |
T6006 |
T6004 |
punct |
),dA |
R4036 |
T6007 |
T6004 |
punct |
", ",dA |
R4037 |
T6008 |
T6009 |
nmod |
poly,dC |
R4038 |
T6009 |
T6004 |
conj |
dC,dA |
R4039 |
T6010 |
T6009 |
punct |
(,dC |
R4040 |
T6011 |
T6009 |
punct |
),dC |
R4041 |
T6012 |
T6009 |
cc |
and,dC |
R4042 |
T6013 |
T6014 |
nmod |
poly,dG |
R4043 |
T6014 |
T6009 |
conj |
dG,dC |
R4044 |
T6015 |
T6014 |
punct |
(,dG |
R4045 |
T6016 |
T6014 |
punct |
),dG |
R4046 |
T6017 |
T6000 |
nummod |
3,tails |
R4047 |
T6018 |
T6017 |
punct |
′,3 |
R4048 |
T6019 |
T6000 |
punct |
-,tails |
R4049 |
T6020 |
T5989 |
prep |
in,constructed |
R4050 |
T6021 |
T6020 |
pobj |
order,in |
R4051 |
T6022 |
T6023 |
aux |
to,examine |
R4052 |
T6023 |
T6021 |
acl |
examine,order |
R4053 |
T6024 |
T6025 |
mark |
whether,displace |
R4054 |
T6025 |
T6023 |
ccomp |
displace,examine |
R4055 |
T6026 |
T6027 |
compound |
mouse,Mcm4 |
R4056 |
T6027 |
T6025 |
nsubj |
Mcm4,displace |
R4057 |
T6028 |
T6027 |
punct |
/,Mcm4 |
R4058 |
T6029 |
T6027 |
nummod |
6,Mcm4 |
R4059 |
T6030 |
T6027 |
punct |
/,Mcm4 |
R4060 |
T6031 |
T6027 |
nummod |
7,Mcm4 |
R4061 |
T6032 |
T6025 |
aux |
can,displace |
R4062 |
T6033 |
T6034 |
det |
these,substrates |
R4063 |
T6034 |
T6025 |
dobj |
substrates,displace |
R4064 |
T6035 |
T5989 |
punct |
.,constructed |
R4065 |
T6037 |
T6038 |
nsubj |
We,found |
R4066 |
T6039 |
T6038 |
advmod |
first,found |
R4067 |
T6040 |
T6041 |
mark |
that,binds |
R4068 |
T6041 |
T6038 |
ccomp |
binds,found |
R4069 |
T6042 |
T6041 |
nsubj |
Mcm4,binds |
R4070 |
T6043 |
T6042 |
punct |
/,Mcm4 |
R4071 |
T6044 |
T6042 |
nummod |
6,Mcm4 |
R4072 |
T6045 |
T6042 |
punct |
/,Mcm4 |
R4073 |
T6046 |
T6042 |
nummod |
7,Mcm4 |
R4074 |
T6047 |
T6041 |
prep |
to,binds |
R4075 |
T6048 |
T6049 |
nmod |
dT,extension |
R4076 |
T6049 |
T6047 |
pobj |
extension,to |
R4077 |
T6050 |
T6048 |
punct |
-,dT |
R4078 |
T6051 |
T6048 |
punct |
", ",dT |
R4079 |
T6052 |
T6048 |
conj |
dC,dT |
R4080 |
T6053 |
T6052 |
punct |
-,dC |
R4081 |
T6054 |
T6052 |
cc |
and,dC |
R4082 |
T6055 |
T6052 |
conj |
dG,dC |
R4083 |
T6056 |
T6049 |
punct |
-,extension |
R4084 |
T6057 |
T6049 |
nummod |
3,extension |
R4085 |
T6058 |
T6057 |
punct |
′,3 |
R4086 |
T6059 |
T6049 |
punct |
-,extension |
R4087 |
T6060 |
T6041 |
prep |
with,binds |
R4088 |
T6061 |
T6062 |
amod |
similar,affinity |
R4089 |
T6062 |
T6060 |
pobj |
affinity,with |
R4090 |
T6063 |
T6041 |
punct |
", ",binds |
R4091 |
T6064 |
T6041 |
cc |
and,binds |
R4092 |
T6065 |
T6041 |
conj |
to,binds |
R4093 |
T6066 |
T6067 |
nmod |
dA,extension |
R4094 |
T6067 |
T6065 |
pobj |
extension,to |
R4095 |
T6068 |
T6067 |
punct |
-,extension |
R4096 |
T6069 |
T6067 |
nummod |
3,extension |
R4097 |
T6070 |
T6069 |
punct |
′,3 |
R4098 |
T6071 |
T6067 |
punct |
-,extension |
R4099 |
T6072 |
T6065 |
prep |
with,to |
R4100 |
T6073 |
T6074 |
advmod |
much,lower |
R4101 |
T6074 |
T6075 |
amod |
lower,affinity |
R4102 |
T6075 |
T6072 |
pobj |
affinity,with |
R4103 |
T6076 |
T6077 |
punct |
(,5A |
R4104 |
T6077 |
T6065 |
parataxis |
5A,to |
R4105 |
T6078 |
T6077 |
compound |
Figure,5A |
R4106 |
T6079 |
T6077 |
punct |
),5A |
R4107 |
T6080 |
T6038 |
punct |
.,found |
R4108 |
T6082 |
T6083 |
nsubj |
This,is |
R4109 |
T6084 |
T6083 |
acomp |
consistent,is |
R4110 |
T6085 |
T6084 |
prep |
with,consistent |
R4111 |
T6086 |
T6087 |
det |
the,results |
R4112 |
T6087 |
T6085 |
pobj |
results,with |
R4113 |
T6088 |
T6087 |
amod |
previous,results |
R4114 |
T6089 |
T6090 |
mark |
that,binds |
R4115 |
T6090 |
T6087 |
acl |
binds,results |
R4116 |
T6091 |
T6090 |
nsubj |
Mcm4,binds |
R4117 |
T6092 |
T6091 |
punct |
/,Mcm4 |
R4118 |
T6093 |
T6091 |
nummod |
6,Mcm4 |
R4119 |
T6094 |
T6091 |
punct |
/,Mcm4 |
R4120 |
T6095 |
T6091 |
nummod |
7,Mcm4 |
R4121 |
T6096 |
T6090 |
prep |
to,binds |
R4122 |
T6097 |
T6098 |
compound |
50mer,dT |
R4123 |
T6098 |
T6096 |
pobj |
dT,to |
R4124 |
T6099 |
T6098 |
compound |
oligo,dT |
R4125 |
T6100 |
T6098 |
punct |
-,dT |
R4126 |
T6101 |
T6098 |
punct |
", ",dT |
R4127 |
T6102 |
T6103 |
punct |
-,dC |
R4128 |
T6103 |
T6098 |
conj |
dC,dT |
R4129 |
T6104 |
T6103 |
cc |
and,dC |
R4130 |
T6105 |
T6106 |
punct |
-,dG |
R4131 |
T6106 |
T6103 |
conj |
dG,dC |
R4132 |
T6107 |
T6096 |
punct |
", ",to |
R4133 |
T6108 |
T6096 |
cc |
but,to |
R4134 |
T6109 |
T6108 |
neg |
not,but |
R4135 |
T6110 |
T6096 |
conj |
to,to |
R4136 |
T6111 |
T6112 |
det |
a,dA |
R4137 |
T6112 |
T6110 |
pobj |
dA,to |
R4138 |
T6113 |
T6112 |
compound |
50mer,dA |
R4139 |
T6114 |
T6112 |
compound |
oligo,dA |
R4140 |
T6115 |
T6112 |
punct |
-,dA |
R4141 |
T6116 |
T6117 |
punct |
(,13 |
R4142 |
T6117 |
T6083 |
parataxis |
13,is |
R4143 |
T6118 |
T6117 |
punct |
),13 |
R4144 |
T6119 |
T6083 |
punct |
.,is |
R4145 |
T6121 |
T6122 |
nsubj |
We,found |
R4146 |
T6123 |
T6124 |
mark |
that,displaced |
R4147 |
T6124 |
T6122 |
ccomp |
displaced,found |
R4148 |
T6125 |
T6126 |
npadvmod |
dT,tailed |
R4149 |
T6126 |
T6128 |
amod |
tailed,extension |
R4150 |
T6127 |
T6126 |
punct |
-,tailed |
R4151 |
T6128 |
T6124 |
nsubjpass |
extension,displaced |
R4152 |
T6129 |
T6128 |
nummod |
3,extension |
R4153 |
T6130 |
T6129 |
punct |
′,3 |
R4154 |
T6131 |
T6128 |
punct |
-,extension |
R4155 |
T6132 |
T6124 |
auxpass |
was,displaced |
R4156 |
T6133 |
T6124 |
advmod |
efficiently,displaced |
R4157 |
T6134 |
T6124 |
agent |
by,displaced |
R4158 |
T6135 |
T6134 |
pobj |
Mcm,by |
R4159 |
T6136 |
T6135 |
nummod |
4,Mcm |
R4160 |
T6137 |
T6135 |
punct |
/,Mcm |
R4161 |
T6138 |
T6135 |
nummod |
6,Mcm |
R4162 |
T6139 |
T6135 |
punct |
/,Mcm |
R4163 |
T6140 |
T6135 |
nummod |
7,Mcm |
R4164 |
T6141 |
T6142 |
mark |
while,detected |
R4165 |
T6142 |
T6124 |
advcl |
detected,displaced |
R4166 |
T6143 |
T6144 |
advmod |
almost,no |
R4167 |
T6144 |
T6142 |
nsubjpass |
no,detected |
R4168 |
T6145 |
T6144 |
cc |
or,no |
R4169 |
T6146 |
T6147 |
advmod |
very,little |
R4170 |
T6147 |
T6148 |
nmod |
little,activity |
R4171 |
T6148 |
T6144 |
conj |
activity,no |
R4172 |
T6149 |
T6142 |
auxpass |
was,detected |
R4173 |
T6150 |
T6142 |
prep |
on,detected |
R4174 |
T6151 |
T6152 |
amod |
other,substrates |
R4175 |
T6152 |
T6150 |
pobj |
substrates,on |
R4176 |
T6153 |
T6154 |
punct |
(,5B |
R4177 |
T6154 |
T6142 |
parataxis |
5B,detected |
R4178 |
T6155 |
T6154 |
compound |
Figure,5B |
R4179 |
T6156 |
T6154 |
cc |
and,5B |
R4180 |
T6157 |
T6154 |
conj |
C,5B |
R4181 |
T6158 |
T6154 |
punct |
),5B |
R4182 |
T6159 |
T6122 |
punct |
.,found |
R4183 |
T6161 |
T6162 |
nsubj |
This,is |
R4184 |
T6163 |
T6162 |
prep |
in,is |
R4185 |
T6164 |
T6165 |
amod |
sharp,contrast |
R4186 |
T6165 |
T6163 |
pobj |
contrast,in |
R4187 |
T6166 |
T6165 |
prep |
to,contrast |
R4188 |
T6167 |
T6168 |
nmod |
yeast,helicases |
R4189 |
T6168 |
T6166 |
pobj |
helicases,to |
R4190 |
T6169 |
T6168 |
nmod |
Mcm4,helicases |
R4191 |
T6170 |
T6169 |
punct |
/,Mcm4 |
R4192 |
T6171 |
T6169 |
nummod |
6,Mcm4 |
R4193 |
T6172 |
T6169 |
punct |
/,Mcm4 |
R4194 |
T6173 |
T6169 |
nummod |
7,Mcm4 |
R4195 |
T6174 |
T6175 |
dep |
which,are |
R4196 |
T6175 |
T6168 |
relcl |
are,helicases |
R4197 |
T6176 |
T6175 |
neg |
not,are |
R4198 |
T6177 |
T6175 |
acomp |
capable,are |
R4199 |
T6178 |
T6177 |
prep |
of,capable |
R4200 |
T6179 |
T6178 |
pcomp |
unwinding,of |
R4201 |
T6180 |
T6181 |
nummod |
3,extension |
R4202 |
T6181 |
T6184 |
compound |
extension,substrates |
R4203 |
T6182 |
T6180 |
punct |
′,3 |
R4204 |
T6183 |
T6181 |
punct |
-,extension |
R4205 |
T6184 |
T6179 |
dobj |
substrates,unwinding |
R4206 |
T6185 |
T6162 |
punct |
.,is |
R4207 |
T6187 |
T6188 |
advmod |
Furthermore,displaced |
R4208 |
T6189 |
T6188 |
punct |
", ",displaced |
R4209 |
T6190 |
T6191 |
npadvmod |
dT,tailed |
R4210 |
T6191 |
T6193 |
amod |
tailed,extension |
R4211 |
T6192 |
T6191 |
punct |
-,tailed |
R4212 |
T6193 |
T6188 |
nsubjpass |
extension,displaced |
R4213 |
T6194 |
T6193 |
nummod |
5,extension |
R4214 |
T6195 |
T6194 |
punct |
′,5 |
R4215 |
T6196 |
T6193 |
punct |
-,extension |
R4216 |
T6197 |
T6193 |
punct |
", ",extension |
R4217 |
T6198 |
T6199 |
prep |
to,bind |
R4218 |
T6199 |
T6193 |
relcl |
bind,extension |
R4219 |
T6200 |
T6198 |
pobj |
which,to |
R4220 |
T6201 |
T6199 |
nsubj |
Mcm4,bind |
R4221 |
T6202 |
T6201 |
punct |
/,Mcm4 |
R4222 |
T6203 |
T6201 |
nummod |
6,Mcm4 |
R4223 |
T6204 |
T6201 |
punct |
/,Mcm4 |
R4224 |
T6205 |
T6201 |
nummod |
7,Mcm4 |
R4225 |
T6206 |
T6199 |
aux |
could,bind |
R4226 |
T6207 |
T6188 |
punct |
", ",displaced |
R4227 |
T6208 |
T6188 |
auxpass |
was,displaced |
R4228 |
T6209 |
T6188 |
neg |
not,displaced |
R4229 |
T6210 |
T6188 |
agent |
by,displaced |
R4230 |
T6211 |
T6212 |
det |
the,helicase |
R4231 |
T6212 |
T6210 |
pobj |
helicase,by |
R4232 |
T6213 |
T6212 |
compound |
Mcm,helicase |
R4233 |
T6214 |
T6188 |
punct |
.,displaced |
R4234 |
T6216 |
T6217 |
det |
The,addition |
R4235 |
T6217 |
T6218 |
nsubj |
addition,stimulated |
R4236 |
T6219 |
T6217 |
prep |
of,addition |
R4237 |
T6220 |
T6221 |
det |
the,tail |
R4238 |
T6221 |
T6219 |
pobj |
tail,of |
R4239 |
T6222 |
T6221 |
nummod |
5,tail |
R4240 |
T6223 |
T6222 |
punct |
′,5 |
R4241 |
T6224 |
T6221 |
punct |
-,tail |
R4242 |
T6225 |
T6217 |
prep |
to,addition |
R4243 |
T6226 |
T6227 |
npadvmod |
dT,tailed |
R4244 |
T6227 |
T6229 |
amod |
tailed,extension |
R4245 |
T6228 |
T6227 |
punct |
-,tailed |
R4246 |
T6229 |
T6225 |
pobj |
extension,to |
R4247 |
T6230 |
T6229 |
nummod |
3,extension |
R4248 |
T6231 |
T6230 |
punct |
′,3 |
R4249 |
T6232 |
T6229 |
punct |
-,extension |
R4250 |
T6233 |
T6218 |
advmod |
further,stimulated |
R4251 |
T6234 |
T6235 |
det |
the,displacement |
R4252 |
T6235 |
T6218 |
dobj |
displacement,stimulated |
R4253 |
T6236 |
T6237 |
punct |
(,5D |
R4254 |
T6237 |
T6235 |
parataxis |
5D,displacement |
R4255 |
T6238 |
T6237 |
compound |
Figure,5D |
R4256 |
T6239 |
T6237 |
punct |
),5D |
R4257 |
T6240 |
T6218 |
punct |
", ",stimulated |
R4258 |
T6241 |
T6218 |
advcl |
confirming,stimulated |
R4259 |
T6242 |
T6243 |
det |
the,report |
R4260 |
T6243 |
T6241 |
dobj |
report,confirming |
R4261 |
T6244 |
T6243 |
amod |
previous,report |
R4262 |
T6245 |
T6246 |
mark |
that,facilitate |
R4263 |
T6246 |
T6243 |
acl |
facilitate,report |
R4264 |
T6247 |
T6248 |
det |
the,structures |
R4265 |
T6248 |
T6246 |
nsubj |
structures,facilitate |
R4266 |
T6249 |
T6248 |
compound |
fork,structures |
R4267 |
T6250 |
T6251 |
det |
the,action |
R4268 |
T6251 |
T6246 |
dobj |
action,facilitate |
R4269 |
T6252 |
T6251 |
compound |
helicase,action |
R4270 |
T6253 |
T6218 |
punct |
.,stimulated |
R4271 |
T6255 |
T6256 |
advmod |
Thus,strengthen |
R4272 |
T6257 |
T6256 |
punct |
", ",strengthen |
R4273 |
T6258 |
T6259 |
det |
these,results |
R4274 |
T6259 |
T6256 |
nsubj |
results,strengthen |
R4275 |
T6260 |
T6256 |
advmod |
further,strengthen |
R4276 |
T6261 |
T6262 |
poss |
our,conclusion |
R4277 |
T6262 |
T6256 |
dobj |
conclusion,strengthen |
R4278 |
T6263 |
T6264 |
mark |
that,activated |
R4279 |
T6264 |
T6262 |
acl |
activated,conclusion |
R4280 |
T6265 |
T6266 |
det |
the,helicase |
R4281 |
T6266 |
T6264 |
nsubjpass |
helicase,activated |
R4282 |
T6267 |
T6266 |
amod |
mammalian,helicase |
R4283 |
T6268 |
T6266 |
compound |
Mcm,helicase |
R4284 |
T6269 |
T6264 |
auxpass |
is,activated |
R4285 |
T6270 |
T6264 |
advmod |
generally,activated |
R4286 |
T6271 |
T6264 |
agent |
by,activated |
R4287 |
T6272 |
T6273 |
det |
the,stretch |
R4288 |
T6273 |
T6271 |
pobj |
stretch,by |
R4289 |
T6274 |
T6273 |
compound |
thymine,stretch |
R4290 |
T6275 |
T6273 |
punct |
-,stretch |
R4291 |
T6276 |
T6273 |
prep |
on,stretch |
R4292 |
T6277 |
T6278 |
det |
the,tail |
R4293 |
T6278 |
T6276 |
pobj |
tail,on |
R4294 |
T6279 |
T6278 |
nummod |
3,tail |
R4295 |
T6280 |
T6279 |
punct |
′,3 |
R4296 |
T6281 |
T6278 |
punct |
-,tail |
R4297 |
T6282 |
T6283 |
amod |
single,stranded |
R4298 |
T6283 |
T6278 |
amod |
stranded,tail |
R4299 |
T6284 |
T6283 |
punct |
-,stranded |
R4300 |
T6285 |
T6256 |
punct |
.,strengthen |