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PMC:1140370 / 17348-19199 JSONTXT 5 Projects

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Id Subject Object Predicate Lexical cue
T5260 0-7 NN denotes Binding
T5262 8-11 CC denotes and
T5263 12-20 NN denotes helicase
T5261 21-28 NNS denotes actions
T5264 29-31 IN denotes of
T5265 32-36 NN denotes Mcm4
T5266 36-37 HYPH denotes /
T5267 37-38 CD denotes 6
T5268 38-39 HYPH denotes /
T5269 39-40 CD denotes 7
T5270 41-43 IN denotes on
T5271 44-50 VBN denotes forked
T5273 51-54 CC denotes and
T5274 55-56 CD denotes 3
T5276 56-57 SYM denotes ′
T5277 57-58 HYPH denotes -
T5278 59-61 CC denotes or
T5279 62-63 CD denotes 5
T5280 63-64 SYM denotes ′
T5281 64-65 HYPH denotes -
T5275 65-74 NN denotes extension
T5272 75-85 NNS denotes substrates
T5282 85-388 sentence denotes In order to examine more precisely the interaction and helicase actions with forked substrates, Y-fork substrates containing a nascent leading strand (A-fork[3′]), nascent lagging strand (A-fork[5′]), or both of them (A-fork[3′,5′]), as well as 5′-extension or 3′-extension structures were constructed.
T5283 86-88 IN denotes In
T5285 89-94 NN denotes order
T5286 95-97 TO denotes to
T5287 98-105 VB denotes examine
T5288 106-110 RBR denotes more
T5289 111-120 RB denotes precisely
T5290 121-124 DT denotes the
T5291 125-136 NN denotes interaction
T5292 137-140 CC denotes and
T5293 141-149 NN denotes helicase
T5294 150-157 NNS denotes actions
T5295 158-162 IN denotes with
T5296 163-169 VBN denotes forked
T5297 170-180 NNS denotes substrates
T5298 180-182 , denotes ,
T5299 182-183 NN denotes Y
T5301 183-184 HYPH denotes -
T5300 184-188 NN denotes fork
T5302 189-199 NNS denotes substrates
T5303 200-210 VBG denotes containing
T5304 211-212 DT denotes a
T5306 213-220 JJ denotes nascent
T5307 221-228 VBG denotes leading
T5305 229-235 NN denotes strand
T5308 236-237 -LRB- denotes (
T5309 237-238 NN denotes A
T5311 238-239 HYPH denotes -
T5310 239-243 NN denotes fork
T5312 243-244 -LRB- denotes [
T5313 244-245 CD denotes 3
T5314 245-246 SYM denotes ′
T5315 246-247 -RRB- denotes ]
T5316 247-248 -RRB- denotes )
T5317 248-250 , denotes ,
T5318 250-257 JJ denotes nascent
T5320 258-265 VBG denotes lagging
T5319 266-272 NN denotes strand
T5321 273-274 -LRB- denotes (
T5322 274-275 NN denotes A
T5324 275-276 HYPH denotes -
T5323 276-280 NN denotes fork
T5325 280-281 -LRB- denotes [
T5326 281-282 CD denotes 5
T5327 282-283 SYM denotes ′
T5328 283-284 -RRB- denotes ]
T5329 284-285 -RRB- denotes )
T5330 285-287 , denotes ,
T5331 287-289 CC denotes or
T5332 290-294 DT denotes both
T5333 295-297 IN denotes of
T5334 298-302 PRP denotes them
T5335 303-304 -LRB- denotes (
T5336 304-305 NN denotes A
T5338 305-306 HYPH denotes -
T5337 306-310 NN denotes fork
T5340 310-311 -LRB- denotes [
T5341 311-312 CD denotes 3
T5342 312-313 SYM denotes ′
T5343 313-314 , denotes ,
T5344 314-315 CD denotes 5
T5345 315-316 SYM denotes ′
T5346 316-317 -RRB- denotes ]
T5347 317-318 -RRB- denotes )
T5348 318-320 , denotes ,
T5349 320-322 RB denotes as
T5351 323-327 RB denotes well
T5350 328-330 IN denotes as
T5352 331-332 CD denotes 5
T5354 332-333 SYM denotes ′
T5355 333-334 HYPH denotes -
T5353 334-343 NN denotes extension
T5356 344-346 CC denotes or
T5357 347-348 CD denotes 3
T5359 348-349 SYM denotes ′
T5360 349-350 HYPH denotes -
T5358 350-359 NN denotes extension
T5339 360-370 NNS denotes structures
T5361 371-375 VBD denotes were
T5284 376-387 VBN denotes constructed
T5362 387-388 . denotes .
T5363 388-512 sentence denotes Mcm4/6/7 bound to A-fork[3′] or A-fork[5′] with affinity similar to that of a simple Y-fork (Figure 4A and data not shown).
T5364 389-393 NN denotes Mcm4
T5366 393-394 HYPH denotes /
T5367 394-395 CD denotes 6
T5368 395-396 HYPH denotes /
T5369 396-397 CD denotes 7
T5365 398-403 VBD denotes bound
T5370 404-406 IN denotes to
T5371 407-408 NN denotes A
T5373 408-409 HYPH denotes -
T5372 409-413 NN denotes fork
T5374 413-414 -LRB- denotes [
T5375 414-415 CD denotes 3
T5376 415-416 SYM denotes ′
T5377 416-417 -RRB- denotes ]
T5378 418-420 CC denotes or
T5379 421-422 NN denotes A
T5381 422-423 HYPH denotes -
T5380 423-427 NN denotes fork
T5382 427-428 -LRB- denotes [
T5383 428-429 CD denotes 5
T5384 429-430 SYM denotes ′
T5385 430-431 -RRB- denotes ]
T5386 432-436 IN denotes with
T5387 437-445 NN denotes affinity
T5388 446-453 JJ denotes similar
T5389 454-456 IN denotes to
T5390 457-461 DT denotes that
T5391 462-464 IN denotes of
T5392 465-466 DT denotes a
T5394 467-473 JJ denotes simple
T5395 474-475 NN denotes Y
T5396 475-476 HYPH denotes -
T5393 476-480 NN denotes fork
T5397 481-482 -LRB- denotes (
T5399 482-488 NN denotes Figure
T5398 489-491 NN denotes 4A
T5400 492-495 CC denotes and
T5401 496-500 NNS denotes data
T5403 501-504 RB denotes not
T5402 505-510 VBN denotes shown
T5404 510-511 -RRB- denotes )
T5405 511-512 . denotes .
T5406 512-648 sentence denotes In contrast, PriA helicase, used as a control, bound to A-fork[3′] more efficiently than to A-fork[5′], as reported previously (23,25).
T5407 513-515 IN denotes In
T5409 516-524 NN denotes contrast
T5410 524-526 , denotes ,
T5411 526-530 NN denotes PriA
T5412 531-539 NN denotes helicase
T5413 539-541 , denotes ,
T5414 541-545 VBN denotes used
T5415 546-548 IN denotes as
T5416 549-550 DT denotes a
T5417 551-558 NN denotes control
T5418 558-560 , denotes ,
T5408 560-565 VBD denotes bound
T5419 566-568 IN denotes to
T5420 569-570 NN denotes A
T5422 570-571 HYPH denotes -
T5421 571-575 NN denotes fork
T5423 575-576 -LRB- denotes [
T5424 576-577 CD denotes 3
T5425 577-578 SYM denotes ′
T5426 578-579 -RRB- denotes ]
T5427 580-584 RBR denotes more
T5428 585-596 RB denotes efficiently
T5429 597-601 IN denotes than
T5430 602-604 IN denotes to
T5431 605-606 NN denotes A
T5433 606-607 HYPH denotes -
T5432 607-611 NN denotes fork
T5434 611-612 -LRB- denotes [
T5435 612-613 CD denotes 5
T5436 613-614 SYM denotes ′
T5437 614-615 -RRB- denotes ]
T5438 615-617 , denotes ,
T5439 617-619 IN denotes as
T5440 620-628 VBN denotes reported
T5441 629-639 RB denotes previously
T5442 640-641 -LRB- denotes (
T5444 641-643 CD denotes 23
T5445 643-644 , denotes ,
T5443 644-646 CD denotes 25
T5446 646-647 -RRB- denotes )
T5447 647-648 . denotes .
T5448 648-791 sentence denotes Mcm4/6/7 bound also to 5′-extension and 3′-extension, albeit with slightly reduced affinity compared to A-fork[3′] and A-fork[5′] (Figure 4A).
T5449 649-653 NN denotes Mcm4
T5451 653-654 HYPH denotes /
T5452 654-655 CD denotes 6
T5453 655-656 HYPH denotes /
T5454 656-657 CD denotes 7
T5450 658-663 VBD denotes bound
T5455 664-668 RB denotes also
T5456 669-671 IN denotes to
T5457 672-673 CD denotes 5
T5459 673-674 SYM denotes ′
T5460 674-675 HYPH denotes -
T5458 675-684 NN denotes extension
T5461 685-688 CC denotes and
T5462 689-690 CD denotes 3
T5464 690-691 SYM denotes ′
T5465 691-692 HYPH denotes -
T5463 692-701 NN denotes extension
T5466 701-703 , denotes ,
T5467 703-709 IN denotes albeit
T5468 710-714 IN denotes with
T5469 715-723 RB denotes slightly
T5470 724-731 VBN denotes reduced
T5471 732-740 NN denotes affinity
T5472 741-749 VBN denotes compared
T5473 750-752 IN denotes to
T5474 753-754 NN denotes A
T5476 754-755 HYPH denotes -
T5475 755-759 NN denotes fork
T5477 759-760 -LRB- denotes [
T5478 760-761 CD denotes 3
T5479 761-762 SYM denotes ′
T5480 762-763 -RRB- denotes ]
T5481 764-767 CC denotes and
T5482 768-769 NN denotes A
T5484 769-770 HYPH denotes -
T5483 770-774 NN denotes fork
T5485 774-775 -LRB- denotes [
T5486 775-776 CD denotes 5
T5487 776-777 SYM denotes ′
T5488 777-778 -RRB- denotes ]
T5489 779-780 -LRB- denotes (
T5491 780-786 NN denotes Figure
T5490 787-789 NN denotes 4A
T5492 789-790 -RRB- denotes )
T5493 790-791 . denotes .
T5494 791-893 sentence denotes Mcm4/6/7 bound to A-fork[3′,5′] with much lower affinity, and the complex gave rise to a single band.
T5495 792-796 NN denotes Mcm4
T5497 796-797 HYPH denotes /
T5498 797-798 CD denotes 6
T5499 798-799 HYPH denotes /
T5500 799-800 CD denotes 7
T5496 801-806 VBD denotes bound
T5501 807-809 IN denotes to
T5502 810-811 NN denotes A
T5504 811-812 HYPH denotes -
T5503 812-816 NN denotes fork
T5505 816-817 -LRB- denotes [
T5506 817-818 CD denotes 3
T5507 818-819 SYM denotes ′
T5508 819-820 , denotes ,
T5509 820-821 CD denotes 5
T5510 821-822 SYM denotes ′
T5511 822-823 -RRB- denotes ]
T5512 824-828 IN denotes with
T5513 829-833 RB denotes much
T5514 834-839 JJR denotes lower
T5515 840-848 NN denotes affinity
T5516 848-850 , denotes ,
T5517 850-853 CC denotes and
T5518 854-857 DT denotes the
T5519 858-865 NN denotes complex
T5520 866-870 VBD denotes gave
T5521 871-875 NN denotes rise
T5522 876-878 IN denotes to
T5523 879-880 DT denotes a
T5525 881-887 JJ denotes single
T5524 888-892 NN denotes band
T5526 892-893 . denotes .
T5527 893-1075 sentence denotes These findings indicate that the Mcm4/6/7 complex is loaded onto DNA preferentially and efficiently through single-stranded region, regardless of the presence of 3′- or 5′-terminus.
T5528 894-899 DT denotes These
T5529 900-908 NNS denotes findings
T5530 909-917 VBP denotes indicate
T5531 918-922 IN denotes that
T5533 923-926 DT denotes the
T5535 927-931 NN denotes Mcm4
T5536 931-932 HYPH denotes /
T5537 932-933 CD denotes 6
T5538 933-934 HYPH denotes /
T5539 934-935 CD denotes 7
T5534 936-943 NN denotes complex
T5540 944-946 VBZ denotes is
T5532 947-953 VBN denotes loaded
T5541 954-958 IN denotes onto
T5542 959-962 NN denotes DNA
T5543 963-977 RB denotes preferentially
T5544 978-981 CC denotes and
T5545 982-993 RB denotes efficiently
T5546 994-1001 IN denotes through
T5547 1002-1008 JJ denotes single
T5549 1008-1009 HYPH denotes -
T5548 1009-1017 VBN denotes stranded
T5550 1018-1024 NN denotes region
T5551 1024-1026 , denotes ,
T5552 1026-1036 RB denotes regardless
T5553 1037-1039 IN denotes of
T5554 1040-1043 DT denotes the
T5555 1044-1052 NN denotes presence
T5556 1053-1055 IN denotes of
T5557 1056-1057 CD denotes 3
T5559 1057-1058 SYM denotes ′
T5560 1058-1059 HYPH denotes -
T5561 1060-1062 CC denotes or
T5562 1063-1064 CD denotes 5
T5563 1064-1065 SYM denotes ′
T5564 1065-1066 HYPH denotes -
T5558 1066-1074 NN denotes terminus
T5565 1074-1075 . denotes .
T5566 1075-1259 sentence denotes In contrast, only the substrate containing a 3′ single-stranded DNA tail (A-fork[5′]) was efficiently unwound and A-fork[3′] or A-fork[3′,5′], was not unwound by Mcm4/6/7 (Figure 4B).
T5567 1076-1078 IN denotes In
T5569 1079-1087 NN denotes contrast
T5570 1087-1089 , denotes ,
T5571 1089-1093 RB denotes only
T5573 1094-1097 DT denotes the
T5572 1098-1107 NN denotes substrate
T5574 1108-1118 VBG denotes containing
T5575 1119-1120 DT denotes a
T5577 1121-1122 CD denotes 3
T5578 1122-1123 SYM denotes ′
T5579 1124-1130 JJ denotes single
T5581 1130-1131 HYPH denotes -
T5580 1131-1139 VBN denotes stranded
T5582 1140-1143 NN denotes DNA
T5576 1144-1148 NN denotes tail
T5583 1149-1150 -LRB- denotes (
T5585 1150-1151 NN denotes A
T5586 1151-1152 HYPH denotes -
T5584 1152-1156 NN denotes fork
T5587 1156-1157 -LRB- denotes [
T5588 1157-1158 CD denotes 5
T5589 1158-1159 SYM denotes ′
T5590 1159-1160 -RRB- denotes ]
T5591 1160-1161 -RRB- denotes )
T5592 1162-1165 VBD denotes was
T5593 1166-1177 RB denotes efficiently
T5568 1178-1185 VBN denotes unwound
T5594 1186-1189 CC denotes and
T5595 1190-1191 NN denotes A
T5597 1191-1192 HYPH denotes -
T5596 1192-1196 NN denotes fork
T5599 1196-1197 -LRB- denotes [
T5600 1197-1198 CD denotes 3
T5601 1198-1199 SYM denotes ′
T5602 1199-1200 -RRB- denotes ]
T5603 1201-1203 CC denotes or
T5604 1204-1205 NN denotes A
T5606 1205-1206 HYPH denotes -
T5605 1206-1210 NN denotes fork
T5607 1210-1211 -LRB- denotes [
T5608 1211-1212 CD denotes 3
T5609 1212-1213 SYM denotes ′
T5610 1213-1214 , denotes ,
T5611 1214-1215 CD denotes 5
T5612 1215-1216 SYM denotes ′
T5613 1216-1217 -RRB- denotes ]
T5614 1217-1219 , denotes ,
T5615 1219-1222 VBD denotes was
T5616 1223-1226 RB denotes not
T5598 1227-1234 VBN denotes unwound
T5617 1235-1237 IN denotes by
T5618 1238-1242 NN denotes Mcm4
T5619 1242-1243 HYPH denotes /
T5620 1243-1244 CD denotes 6
T5621 1244-1245 HYPH denotes /
T5622 1245-1246 CD denotes 7
T5623 1247-1248 -LRB- denotes (
T5625 1248-1254 NN denotes Figure
T5624 1255-1257 NN denotes 4B
T5626 1257-1258 -RRB- denotes )
T5627 1258-1259 . denotes .
T5628 1259-1436 sentence denotes Absence of helicase actions on A-fork[3′,5′] composed only of duplex regions is expected since double-stranded DNA is not able to activate the ATPase activity of Mcm4/6/7 (10).
T5629 1260-1267 NN denotes Absence
T5631 1268-1270 IN denotes of
T5632 1271-1279 NN denotes helicase
T5633 1280-1287 NNS denotes actions
T5634 1288-1290 IN denotes on
T5635 1291-1292 NN denotes A
T5637 1292-1293 HYPH denotes -
T5636 1293-1297 NN denotes fork
T5638 1297-1298 -LRB- denotes [
T5639 1298-1299 CD denotes 3
T5640 1299-1300 SYM denotes ′
T5641 1300-1301 , denotes ,
T5642 1301-1302 CD denotes 5
T5643 1302-1303 SYM denotes ′
T5644 1303-1304 -RRB- denotes ]
T5645 1305-1313 VBN denotes composed
T5646 1314-1318 RB denotes only
T5647 1319-1321 IN denotes of
T5648 1322-1328 NN denotes duplex
T5649 1329-1336 NNS denotes regions
T5650 1337-1339 VBZ denotes is
T5630 1340-1348 VBN denotes expected
T5651 1349-1354 IN denotes since
T5653 1355-1361 JJ denotes double
T5655 1361-1362 HYPH denotes -
T5654 1362-1370 VBN denotes stranded
T5656 1371-1374 NN denotes DNA
T5652 1375-1377 VBZ denotes is
T5657 1378-1381 RB denotes not
T5658 1382-1386 JJ denotes able
T5659 1387-1389 TO denotes to
T5660 1390-1398 VB denotes activate
T5661 1399-1402 DT denotes the
T5663 1403-1409 NN denotes ATPase
T5662 1410-1418 NN denotes activity
T5664 1419-1421 IN denotes of
T5665 1422-1426 NN denotes Mcm4
T5666 1426-1427 HYPH denotes /
T5667 1427-1428 CD denotes 6
T5668 1428-1429 HYPH denotes /
T5669 1429-1430 CD denotes 7
T5670 1431-1432 -LRB- denotes (
T5671 1432-1434 CD denotes 10
T5672 1434-1435 -RRB- denotes )
T5673 1435-1436 . denotes .
T5674 1436-1687 sentence denotes In contrast, the PriA, a structure-specific helicase used as a control, binds at the fork junction and unwinds the fork and the nascent lagging strand DNA on A-fork[5′], or unwinds the nascent lagging strand on A-fork[3′,5′], as reported before (25).
T5675 1437-1439 IN denotes In
T5677 1440-1448 NN denotes contrast
T5678 1448-1450 , denotes ,
T5679 1450-1453 DT denotes the
T5680 1454-1458 NN denotes PriA
T5681 1458-1460 , denotes ,
T5682 1460-1461 DT denotes a
T5684 1462-1471 NN denotes structure
T5686 1471-1472 HYPH denotes -
T5685 1472-1480 JJ denotes specific
T5683 1481-1489 NN denotes helicase
T5687 1490-1494 VBN denotes used
T5688 1495-1497 IN denotes as
T5689 1498-1499 DT denotes a
T5690 1500-1507 NN denotes control
T5691 1507-1509 , denotes ,
T5676 1509-1514 VBZ denotes binds
T5692 1515-1517 IN denotes at
T5693 1518-1521 DT denotes the
T5695 1522-1526 NN denotes fork
T5694 1527-1535 NN denotes junction
T5696 1536-1539 CC denotes and
T5697 1540-1547 VBZ denotes unwinds
T5698 1548-1551 DT denotes the
T5699 1552-1556 NN denotes fork
T5700 1557-1560 CC denotes and
T5701 1561-1564 DT denotes the
T5703 1565-1572 JJ denotes nascent
T5704 1573-1580 VBG denotes lagging
T5705 1581-1587 NN denotes strand
T5702 1588-1591 NN denotes DNA
T5706 1592-1594 IN denotes on
T5707 1595-1596 NN denotes A
T5709 1596-1597 HYPH denotes -
T5708 1597-1601 NN denotes fork
T5710 1601-1602 -LRB- denotes [
T5711 1602-1603 CD denotes 5
T5712 1603-1604 SYM denotes ′
T5713 1604-1605 -RRB- denotes ]
T5714 1605-1607 , denotes ,
T5715 1607-1609 CC denotes or
T5716 1610-1617 VBZ denotes unwinds
T5717 1618-1621 DT denotes the
T5719 1622-1629 JJ denotes nascent
T5720 1630-1637 VBG denotes lagging
T5718 1638-1644 NN denotes strand
T5721 1645-1647 IN denotes on
T5722 1648-1649 NN denotes A
T5724 1649-1650 HYPH denotes -
T5723 1650-1654 NN denotes fork
T5725 1654-1655 -LRB- denotes [
T5726 1655-1656 CD denotes 3
T5727 1656-1657 SYM denotes ′
T5728 1657-1658 , denotes ,
T5729 1658-1659 CD denotes 5
T5730 1659-1660 SYM denotes ′
T5731 1660-1661 -RRB- denotes ]
T5732 1661-1663 , denotes ,
T5733 1663-1665 IN denotes as
T5734 1666-1674 VBN denotes reported
T5735 1675-1681 RB denotes before
T5736 1682-1683 -LRB- denotes (
T5737 1683-1685 CD denotes 25
T5738 1685-1686 -RRB- denotes )
T5739 1686-1687 . denotes .
T5740 1687-1851 sentence denotes These results indicate that the Mcm4/6/7 complex binds to single-stranded DNA and that its helicase is activated only in the presence of a single-stranded 3′-tail.
T5741 1688-1693 DT denotes These
T5742 1694-1701 NNS denotes results
T5743 1702-1710 VBP denotes indicate
T5744 1711-1715 IN denotes that
T5746 1716-1719 DT denotes the
T5748 1720-1724 NN denotes Mcm4
T5749 1724-1725 HYPH denotes /
T5750 1725-1726 CD denotes 6
T5751 1726-1727 HYPH denotes /
T5752 1727-1728 CD denotes 7
T5747 1729-1736 NN denotes complex
T5745 1737-1742 VBZ denotes binds
T5753 1743-1745 IN denotes to
T5754 1746-1752 JJ denotes single
T5756 1752-1753 HYPH denotes -
T5755 1753-1761 VBN denotes stranded
T5757 1762-1765 NN denotes DNA
T5758 1766-1769 CC denotes and
T5759 1770-1774 IN denotes that
T5761 1775-1778 PRP$ denotes its
T5762 1779-1787 NN denotes helicase
T5763 1788-1790 VBZ denotes is
T5760 1791-1800 VBN denotes activated
T5764 1801-1805 RB denotes only
T5765 1806-1808 IN denotes in
T5766 1809-1812 DT denotes the
T5767 1813-1821 NN denotes presence
T5768 1822-1824 IN denotes of
T5769 1825-1826 DT denotes a
T5771 1827-1833 JJ denotes single
T5773 1833-1834 HYPH denotes -
T5772 1834-1842 VBN denotes stranded
T5774 1843-1844 CD denotes 3
T5775 1844-1845 SYM denotes ′
T5776 1845-1846 HYPH denotes -
T5770 1846-1850 NN denotes tail
T5777 1850-1851 . denotes .
R3464 T5260 T5261 nmod Binding,actions
R3465 T5262 T5260 cc and,Binding
R3466 T5263 T5260 conj helicase,Binding
R3467 T5264 T5261 prep of,actions
R3468 T5265 T5264 pobj Mcm4,of
R3469 T5266 T5265 punct /,Mcm4
R3470 T5267 T5265 nummod 6,Mcm4
R3471 T5268 T5265 punct /,Mcm4
R3472 T5269 T5265 nummod 7,Mcm4
R3473 T5270 T5261 prep on,actions
R3474 T5271 T5272 amod forked,substrates
R3475 T5272 T5270 pobj substrates,on
R3476 T5273 T5271 cc and,forked
R3477 T5274 T5275 nummod 3,extension
R3478 T5275 T5271 conj extension,forked
R3479 T5276 T5274 punct ′,3
R3480 T5277 T5274 punct -,3
R3481 T5278 T5274 cc or,3
R3482 T5279 T5274 conj 5,3
R3483 T5280 T5279 punct ′,5
R3484 T5281 T5275 punct -,extension
R3485 T5283 T5284 prep In,constructed
R3486 T5285 T5283 pobj order,In
R3487 T5286 T5287 aux to,examine
R3488 T5287 T5285 acl examine,order
R3489 T5288 T5289 advmod more,precisely
R3490 T5289 T5287 advmod precisely,examine
R3491 T5290 T5291 det the,interaction
R3492 T5291 T5287 dobj interaction,examine
R3493 T5292 T5291 cc and,interaction
R3494 T5293 T5294 compound helicase,actions
R3495 T5294 T5291 conj actions,interaction
R3496 T5295 T5291 prep with,interaction
R3497 T5296 T5297 amod forked,substrates
R3498 T5297 T5295 pobj substrates,with
R3499 T5298 T5284 punct ", ",constructed
R3500 T5299 T5300 compound Y,fork
R3501 T5300 T5302 compound fork,substrates
R3502 T5301 T5300 punct -,fork
R3503 T5302 T5284 nsubjpass substrates,constructed
R3504 T5303 T5302 acl containing,substrates
R3505 T5304 T5305 det a,strand
R3506 T5305 T5303 dobj strand,containing
R3507 T5306 T5305 amod nascent,strand
R3508 T5307 T5305 amod leading,strand
R3509 T5308 T5305 punct (,strand
R3510 T5309 T5310 compound A,fork
R3511 T5310 T5305 appos fork,strand
R3512 T5311 T5310 punct -,fork
R3513 T5312 T5310 punct [,fork
R3514 T5313 T5310 nummod 3,fork
R3515 T5314 T5310 punct ′,fork
R3516 T5315 T5305 punct ],strand
R3517 T5316 T5305 punct ),strand
R3518 T5317 T5305 punct ", ",strand
R3519 T5318 T5319 amod nascent,strand
R3520 T5319 T5305 appos strand,strand
R3521 T5320 T5319 amod lagging,strand
R3522 T5321 T5319 punct (,strand
R3523 T5322 T5323 compound A,fork
R3524 T5323 T5319 appos fork,strand
R3525 T5324 T5323 punct -,fork
R3526 T5325 T5323 punct [,fork
R3527 T5326 T5323 nummod 5,fork
R3528 T5327 T5323 punct ′,fork
R3529 T5328 T5305 punct ],strand
R3530 T5329 T5305 punct ),strand
R3531 T5330 T5305 punct ", ",strand
R3532 T5331 T5305 cc or,strand
R3533 T5332 T5305 conj both,strand
R3534 T5333 T5332 prep of,both
R3535 T5334 T5333 pobj them,of
R3536 T5335 T5332 punct (,both
R3537 T5336 T5337 nmod A,fork
R3538 T5337 T5339 nmod fork,structures
R3539 T5338 T5337 punct -,fork
R3540 T5339 T5332 appos structures,both
R3541 T5340 T5337 punct [,fork
R3542 T5341 T5337 appos 3,fork
R3543 T5342 T5341 punct ′,3
R3544 T5343 T5341 punct ",",3
R3545 T5344 T5341 appos 5,3
R3546 T5345 T5344 punct ′,5
R3547 T5346 T5337 punct ],fork
R3548 T5347 T5337 punct ),fork
R3549 T5348 T5337 punct ", ",fork
R3550 T5349 T5350 advmod as,as
R3551 T5350 T5337 cc as,fork
R3552 T5351 T5350 advmod well,as
R3553 T5352 T5353 nummod 5,extension
R3554 T5353 T5337 conj extension,fork
R3555 T5354 T5352 punct ′,5
R3556 T5355 T5353 punct -,extension
R3557 T5356 T5353 cc or,extension
R3558 T5357 T5358 nummod 3,extension
R3559 T5358 T5353 conj extension,extension
R3560 T5359 T5357 punct ′,3
R3561 T5360 T5358 punct -,extension
R3562 T5361 T5284 auxpass were,constructed
R3563 T5362 T5284 punct .,constructed
R3564 T5364 T5365 nsubj Mcm4,bound
R3565 T5366 T5364 punct /,Mcm4
R3566 T5367 T5364 nummod 6,Mcm4
R3567 T5368 T5364 punct /,Mcm4
R3568 T5369 T5364 nummod 7,Mcm4
R3569 T5370 T5365 prep to,bound
R3570 T5371 T5372 compound A,fork
R3571 T5372 T5370 pobj fork,to
R3572 T5373 T5372 punct -,fork
R3573 T5374 T5372 punct [,fork
R3574 T5375 T5372 nummod 3,fork
R3575 T5376 T5372 punct ′,fork
R3576 T5377 T5372 punct ],fork
R3577 T5378 T5372 cc or,fork
R3578 T5379 T5380 compound A,fork
R3579 T5380 T5372 conj fork,fork
R3580 T5381 T5380 punct -,fork
R3581 T5382 T5380 punct [,fork
R3582 T5383 T5380 nummod 5,fork
R3583 T5384 T5380 punct ′,fork
R3584 T5385 T5365 punct ],bound
R3585 T5386 T5365 prep with,bound
R3586 T5387 T5386 pobj affinity,with
R3587 T5388 T5387 amod similar,affinity
R3588 T5389 T5388 prep to,similar
R3589 T5390 T5389 pobj that,to
R3590 T5391 T5390 prep of,that
R3591 T5392 T5393 det a,fork
R3592 T5393 T5391 pobj fork,of
R3593 T5394 T5393 amod simple,fork
R3594 T5395 T5393 compound Y,fork
R3595 T5396 T5393 punct -,fork
R3596 T5397 T5398 punct (,4A
R3597 T5398 T5365 parataxis 4A,bound
R3598 T5399 T5398 compound Figure,4A
R3599 T5400 T5398 cc and,4A
R3600 T5401 T5402 nsubj data,shown
R3601 T5402 T5398 conj shown,4A
R3602 T5403 T5402 neg not,shown
R3603 T5404 T5398 punct ),4A
R3604 T5405 T5365 punct .,bound
R3605 T5407 T5408 prep In,bound
R3606 T5409 T5407 pobj contrast,In
R3607 T5410 T5408 punct ", ",bound
R3608 T5411 T5412 compound PriA,helicase
R3609 T5412 T5408 nsubj helicase,bound
R3610 T5413 T5412 punct ", ",helicase
R3611 T5414 T5412 acl used,helicase
R3612 T5415 T5414 prep as,used
R3613 T5416 T5417 det a,control
R3614 T5417 T5415 pobj control,as
R3615 T5418 T5408 punct ", ",bound
R3616 T5419 T5408 prep to,bound
R3617 T5420 T5421 compound A,fork
R3618 T5421 T5419 pobj fork,to
R3619 T5422 T5421 punct -,fork
R3620 T5423 T5421 punct [,fork
R3621 T5424 T5421 nummod 3,fork
R3622 T5425 T5421 punct ′,fork
R3623 T5426 T5408 punct ],bound
R3624 T5427 T5428 advmod more,efficiently
R3625 T5428 T5408 advmod efficiently,bound
R3626 T5429 T5428 prep than,efficiently
R3627 T5430 T5429 prep to,than
R3628 T5431 T5432 compound A,fork
R3629 T5432 T5430 pobj fork,to
R3630 T5433 T5432 punct -,fork
R3631 T5434 T5432 punct [,fork
R3632 T5435 T5432 nummod 5,fork
R3633 T5436 T5432 punct ′,fork
R3634 T5437 T5408 punct ],bound
R3635 T5438 T5408 punct ", ",bound
R3636 T5439 T5440 mark as,reported
R3637 T5440 T5408 advcl reported,bound
R3638 T5441 T5440 advmod previously,reported
R3639 T5442 T5443 punct (,25
R3640 T5443 T5440 parataxis 25,reported
R3641 T5444 T5443 nummod 23,25
R3642 T5445 T5443 punct ",",25
R3643 T5446 T5443 punct ),25
R3644 T5447 T5408 punct .,bound
R3645 T5449 T5450 nsubj Mcm4,bound
R3646 T5451 T5449 punct /,Mcm4
R3647 T5452 T5449 nummod 6,Mcm4
R3648 T5453 T5449 punct /,Mcm4
R3649 T5454 T5449 nummod 7,Mcm4
R3650 T5455 T5450 advmod also,bound
R3651 T5456 T5450 prep to,bound
R3652 T5457 T5458 nummod 5,extension
R3653 T5458 T5456 pobj extension,to
R3654 T5459 T5457 punct ′,5
R3655 T5460 T5458 punct -,extension
R3656 T5461 T5458 cc and,extension
R3657 T5462 T5463 nummod 3,extension
R3658 T5463 T5458 conj extension,extension
R3659 T5464 T5462 punct ′,3
R3660 T5465 T5463 punct -,extension
R3661 T5466 T5450 punct ", ",bound
R3662 T5467 T5468 dep albeit,with
R3663 T5468 T5450 prep with,bound
R3664 T5469 T5470 advmod slightly,reduced
R3665 T5470 T5471 amod reduced,affinity
R3666 T5471 T5468 pobj affinity,with
R3667 T5472 T5471 prep compared,affinity
R3668 T5473 T5472 prep to,compared
R3669 T5474 T5475 compound A,fork
R3670 T5475 T5473 pobj fork,to
R3671 T5476 T5475 punct -,fork
R3672 T5477 T5475 punct [,fork
R3673 T5478 T5475 nummod 3,fork
R3674 T5479 T5475 punct ′,fork
R3675 T5480 T5475 punct ],fork
R3676 T5481 T5475 cc and,fork
R3677 T5482 T5483 compound A,fork
R3678 T5483 T5475 conj fork,fork
R3679 T5484 T5483 punct -,fork
R3680 T5485 T5483 punct [,fork
R3681 T5486 T5483 nummod 5,fork
R3682 T5487 T5483 punct ′,fork
R3683 T5488 T5450 punct ],bound
R3684 T5489 T5490 punct (,4A
R3685 T5490 T5450 parataxis 4A,bound
R3686 T5491 T5490 compound Figure,4A
R3687 T5492 T5490 punct ),4A
R3688 T5493 T5450 punct .,bound
R3689 T5495 T5496 nsubj Mcm4,bound
R3690 T5497 T5495 punct /,Mcm4
R3691 T5498 T5495 nummod 6,Mcm4
R3692 T5499 T5495 punct /,Mcm4
R3693 T5500 T5495 nummod 7,Mcm4
R3694 T5501 T5496 prep to,bound
R3695 T5502 T5503 compound A,fork
R3696 T5503 T5501 pobj fork,to
R3697 T5504 T5503 punct -,fork
R3698 T5505 T5503 punct [,fork
R3699 T5506 T5503 appos 3,fork
R3700 T5507 T5506 punct ′,3
R3701 T5508 T5506 punct ",",3
R3702 T5509 T5506 appos 5,3
R3703 T5510 T5509 punct ′,5
R3704 T5511 T5496 punct ],bound
R3705 T5512 T5496 prep with,bound
R3706 T5513 T5514 advmod much,lower
R3707 T5514 T5515 amod lower,affinity
R3708 T5515 T5512 pobj affinity,with
R3709 T5516 T5496 punct ", ",bound
R3710 T5517 T5496 cc and,bound
R3711 T5518 T5519 det the,complex
R3712 T5519 T5520 nsubj complex,gave
R3713 T5520 T5496 conj gave,bound
R3714 T5521 T5520 dobj rise,gave
R3715 T5522 T5520 prep to,gave
R3716 T5523 T5524 det a,band
R3717 T5524 T5522 pobj band,to
R3718 T5525 T5524 amod single,band
R3719 T5526 T5520 punct .,gave
R3720 T5528 T5529 det These,findings
R3721 T5529 T5530 nsubj findings,indicate
R3722 T5531 T5532 mark that,loaded
R3723 T5532 T5530 ccomp loaded,indicate
R3724 T5533 T5534 det the,complex
R3725 T5534 T5532 nsubjpass complex,loaded
R3726 T5535 T5534 nmod Mcm4,complex
R3727 T5536 T5535 punct /,Mcm4
R3728 T5537 T5535 nummod 6,Mcm4
R3729 T5538 T5535 punct /,Mcm4
R3730 T5539 T5535 nummod 7,Mcm4
R3731 T5540 T5532 auxpass is,loaded
R3732 T5541 T5532 prep onto,loaded
R3733 T5542 T5541 pobj DNA,onto
R3734 T5543 T5532 advmod preferentially,loaded
R3735 T5544 T5543 cc and,preferentially
R3736 T5545 T5543 conj efficiently,preferentially
R3737 T5546 T5532 prep through,loaded
R3738 T5547 T5548 amod single,stranded
R3739 T5548 T5550 amod stranded,region
R3740 T5549 T5548 punct -,stranded
R3741 T5550 T5546 pobj region,through
R3742 T5551 T5532 punct ", ",loaded
R3743 T5552 T5532 advmod regardless,loaded
R3744 T5553 T5552 prep of,regardless
R3745 T5554 T5555 det the,presence
R3746 T5555 T5553 pobj presence,of
R3747 T5556 T5555 prep of,presence
R3748 T5557 T5558 nummod 3,terminus
R3749 T5558 T5556 pobj terminus,of
R3750 T5559 T5557 punct ′,3
R3751 T5560 T5557 punct -,3
R3752 T5561 T5557 cc or,3
R3753 T5562 T5557 conj 5,3
R3754 T5563 T5562 punct ′,5
R3755 T5564 T5558 punct -,terminus
R3756 T5565 T5530 punct .,indicate
R3757 T5567 T5568 prep In,unwound
R3758 T5569 T5567 pobj contrast,In
R3759 T5570 T5568 punct ", ",unwound
R3760 T5571 T5572 advmod only,substrate
R3761 T5572 T5568 nsubjpass substrate,unwound
R3762 T5573 T5572 det the,substrate
R3763 T5574 T5572 acl containing,substrate
R3764 T5575 T5576 det a,tail
R3765 T5576 T5574 dobj tail,containing
R3766 T5577 T5576 nummod 3,tail
R3767 T5578 T5577 punct ′,3
R3768 T5579 T5580 amod single,stranded
R3769 T5580 T5576 amod stranded,tail
R3770 T5581 T5580 punct -,stranded
R3771 T5582 T5576 compound DNA,tail
R3772 T5583 T5584 punct (,fork
R3773 T5584 T5572 appos fork,substrate
R3774 T5585 T5584 compound A,fork
R3775 T5586 T5584 punct -,fork
R3776 T5587 T5584 punct [,fork
R3777 T5588 T5584 nummod 5,fork
R3778 T5589 T5584 punct ′,fork
R3779 T5590 T5568 punct ],unwound
R3780 T5591 T5568 punct ),unwound
R3781 T5592 T5568 auxpass was,unwound
R3782 T5593 T5568 advmod efficiently,unwound
R3783 T5594 T5568 cc and,unwound
R3784 T5595 T5596 compound A,fork
R3785 T5596 T5598 nsubjpass fork,unwound
R3786 T5597 T5596 punct -,fork
R3787 T5598 T5568 conj unwound,unwound
R3788 T5599 T5596 punct [,fork
R3789 T5600 T5596 nummod 3,fork
R3790 T5601 T5596 punct ′,fork
R3791 T5602 T5596 punct ],fork
R3792 T5603 T5596 cc or,fork
R3793 T5604 T5605 compound A,fork
R3794 T5605 T5596 conj fork,fork
R3795 T5606 T5605 punct -,fork
R3796 T5607 T5605 punct [,fork
R3797 T5608 T5605 appos 3,fork
R3798 T5609 T5608 punct ′,3
R3799 T5610 T5608 punct ",",3
R3800 T5611 T5608 appos 5,3
R3801 T5612 T5611 punct ′,5
R3802 T5613 T5598 punct ],unwound
R3803 T5614 T5598 punct ", ",unwound
R3804 T5615 T5598 auxpass was,unwound
R3805 T5616 T5598 neg not,unwound
R3806 T5617 T5598 agent by,unwound
R3807 T5618 T5617 pobj Mcm4,by
R3808 T5619 T5618 punct /,Mcm4
R3809 T5620 T5618 nummod 6,Mcm4
R3810 T5621 T5618 punct /,Mcm4
R3811 T5622 T5618 nummod 7,Mcm4
R3812 T5623 T5624 punct (,4B
R3813 T5624 T5598 parataxis 4B,unwound
R3814 T5625 T5624 compound Figure,4B
R3815 T5626 T5624 punct ),4B
R3816 T5627 T5568 punct .,unwound
R3817 T5629 T5630 nsubjpass Absence,expected
R3818 T5631 T5629 prep of,Absence
R3819 T5632 T5633 compound helicase,actions
R3820 T5633 T5631 pobj actions,of
R3821 T5634 T5629 prep on,Absence
R3822 T5635 T5636 compound A,fork
R3823 T5636 T5634 pobj fork,on
R3824 T5637 T5636 punct -,fork
R3825 T5638 T5636 punct [,fork
R3826 T5639 T5636 appos 3,fork
R3827 T5640 T5639 punct ′,3
R3828 T5641 T5639 punct ",",3
R3829 T5642 T5639 appos 5,3
R3830 T5643 T5642 punct ′,5
R3831 T5644 T5636 punct ],fork
R3832 T5645 T5636 acl composed,fork
R3833 T5646 T5647 advmod only,of
R3834 T5647 T5645 prep of,composed
R3835 T5648 T5649 compound duplex,regions
R3836 T5649 T5647 pobj regions,of
R3837 T5650 T5630 auxpass is,expected
R3838 T5651 T5652 mark since,is
R3839 T5652 T5630 advcl is,expected
R3840 T5653 T5654 amod double,stranded
R3841 T5654 T5656 amod stranded,DNA
R3842 T5655 T5654 punct -,stranded
R3843 T5656 T5652 nsubj DNA,is
R3844 T5657 T5652 neg not,is
R3845 T5658 T5652 acomp able,is
R3846 T5659 T5660 aux to,activate
R3847 T5660 T5658 xcomp activate,able
R3848 T5661 T5662 det the,activity
R3849 T5662 T5660 dobj activity,activate
R3850 T5663 T5662 compound ATPase,activity
R3851 T5664 T5662 prep of,activity
R3852 T5665 T5664 pobj Mcm4,of
R3853 T5666 T5665 punct /,Mcm4
R3854 T5667 T5665 nummod 6,Mcm4
R3855 T5668 T5665 punct /,Mcm4
R3856 T5669 T5665 nummod 7,Mcm4
R3857 T5670 T5671 punct (,10
R3858 T5671 T5652 parataxis 10,is
R3859 T5672 T5671 punct ),10
R3860 T5673 T5630 punct .,expected
R3861 T5675 T5676 prep In,binds
R3862 T5677 T5675 pobj contrast,In
R3863 T5678 T5676 punct ", ",binds
R3864 T5679 T5680 det the,PriA
R3865 T5680 T5676 nsubj PriA,binds
R3866 T5681 T5680 punct ", ",PriA
R3867 T5682 T5683 det a,helicase
R3868 T5683 T5680 appos helicase,PriA
R3869 T5684 T5685 npadvmod structure,specific
R3870 T5685 T5683 amod specific,helicase
R3871 T5686 T5685 punct -,specific
R3872 T5687 T5683 acl used,helicase
R3873 T5688 T5687 prep as,used
R3874 T5689 T5690 det a,control
R3875 T5690 T5688 pobj control,as
R3876 T5691 T5676 punct ", ",binds
R3877 T5692 T5676 prep at,binds
R3878 T5693 T5694 det the,junction
R3879 T5694 T5692 pobj junction,at
R3880 T5695 T5694 compound fork,junction
R3881 T5696 T5676 cc and,binds
R3882 T5697 T5676 conj unwinds,binds
R3883 T5698 T5699 det the,fork
R3884 T5699 T5697 dobj fork,unwinds
R3885 T5700 T5699 cc and,fork
R3886 T5701 T5702 det the,DNA
R3887 T5702 T5699 conj DNA,fork
R3888 T5703 T5702 amod nascent,DNA
R3889 T5704 T5702 amod lagging,DNA
R3890 T5705 T5702 compound strand,DNA
R3891 T5706 T5702 prep on,DNA
R3892 T5707 T5708 compound A,fork
R3893 T5708 T5706 pobj fork,on
R3894 T5709 T5708 punct -,fork
R3895 T5710 T5708 punct [,fork
R3896 T5711 T5708 nummod 5,fork
R3897 T5712 T5708 punct ′,fork
R3898 T5713 T5676 punct ],binds
R3899 T5714 T5676 punct ", ",binds
R3900 T5715 T5676 cc or,binds
R3901 T5716 T5676 conj unwinds,binds
R3902 T5717 T5718 det the,strand
R3903 T5718 T5716 dobj strand,unwinds
R3904 T5719 T5718 amod nascent,strand
R3905 T5720 T5718 amod lagging,strand
R3906 T5721 T5716 prep on,unwinds
R3907 T5722 T5723 compound A,fork
R3908 T5723 T5721 pobj fork,on
R3909 T5724 T5723 punct -,fork
R3910 T5725 T5723 punct [,fork
R3911 T5726 T5723 appos 3,fork
R3912 T5727 T5726 punct ′,3
R3913 T5728 T5726 punct ",",3
R3914 T5729 T5726 appos 5,3
R3915 T5730 T5729 punct ′,5
R3916 T5731 T5676 punct ],binds
R3917 T5732 T5676 punct ", ",binds
R3918 T5733 T5734 mark as,reported
R3919 T5734 T5676 advcl reported,binds
R3920 T5735 T5734 advmod before,reported
R3921 T5736 T5737 punct (,25
R3922 T5737 T5734 parataxis 25,reported
R3923 T5738 T5737 punct ),25
R3924 T5739 T5676 punct .,binds
R3925 T5741 T5742 det These,results
R3926 T5742 T5743 nsubj results,indicate
R3927 T5744 T5745 mark that,binds
R3928 T5745 T5743 advcl binds,indicate
R3929 T5746 T5747 det the,complex
R3930 T5747 T5745 nsubj complex,binds
R3931 T5748 T5747 nmod Mcm4,complex
R3932 T5749 T5748 punct /,Mcm4
R3933 T5750 T5748 nummod 6,Mcm4
R3934 T5751 T5748 punct /,Mcm4
R3935 T5752 T5748 nummod 7,Mcm4
R3936 T5753 T5745 prep to,binds
R3937 T5754 T5755 amod single,stranded
R3938 T5755 T5757 amod stranded,DNA
R3939 T5756 T5755 punct -,stranded
R3940 T5757 T5753 pobj DNA,to
R3941 T5758 T5745 cc and,binds
R3942 T5759 T5760 mark that,activated
R3943 T5760 T5745 conj activated,binds
R3944 T5761 T5762 poss its,helicase
R3945 T5762 T5760 nsubjpass helicase,activated
R3946 T5763 T5760 auxpass is,activated
R3947 T5764 T5765 advmod only,in
R3948 T5765 T5760 prep in,activated
R3949 T5766 T5767 det the,presence
R3950 T5767 T5765 pobj presence,in
R3951 T5768 T5767 prep of,presence
R3952 T5769 T5770 det a,tail
R3953 T5770 T5768 pobj tail,of
R3954 T5771 T5772 amod single,stranded
R3955 T5772 T5770 amod stranded,tail
R3956 T5773 T5772 punct -,stranded
R3957 T5774 T5770 nummod 3,tail
R3958 T5775 T5774 punct ′,3
R3959 T5776 T5770 punct -,tail
R3960 T5777 T5743 punct .,indicate