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PMC:1140370 / 1525-37852 JSONTXT 6 Projects

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Id Subject Object Predicate Lexical cue
T757 13-16 NN denotes DNA
T758 17-26 NNS denotes helicases
T759 27-33 VBP denotes couple
T760 34-37 DT denotes the
T761 38-48 NN denotes hydrolysis
T762 49-51 IN denotes of
T763 52-62 NN denotes nucleotide
T765 63-65 CC denotes or
T766 66-81 NN denotes deoxynucleotide
T764 82-94 NN denotes triphosphate
T767 95-97 IN denotes to
T768 98-104 NN denotes duplex
T769 105-114 NN denotes unwinding
T770 115-116 -LRB- denotes (
T771 116-117 CD denotes 1
T772 117-118 -RRB- denotes )
T773 118-119 . denotes .
T774 119-266 sentence denotes Coordination of a set of subactivities including nucleotide binding, DNA binding and ATP hydrolysis is required for unwinding of duplex DNA (1,2).
T775 120-132 NN denotes Coordination
T777 133-135 IN denotes of
T778 136-137 DT denotes a
T779 138-141 NN denotes set
T780 142-144 IN denotes of
T781 145-158 NNS denotes subactivities
T782 159-168 VBG denotes including
T783 169-179 NN denotes nucleotide
T784 180-187 NN denotes binding
T785 187-189 , denotes ,
T786 189-192 NN denotes DNA
T787 193-200 NN denotes binding
T788 201-204 CC denotes and
T789 205-208 NN denotes ATP
T790 209-219 NN denotes hydrolysis
T791 220-222 VBZ denotes is
T776 223-231 VBN denotes required
T792 232-235 IN denotes for
T793 236-245 NN denotes unwinding
T794 246-248 IN denotes of
T795 249-255 NN denotes duplex
T796 256-259 NN denotes DNA
T797 260-261 -LRB- denotes (
T799 261-262 CD denotes 1
T800 262-263 , denotes ,
T798 263-264 CD denotes 2
T801 264-265 -RRB- denotes )
T802 265-266 . denotes .
T803 266-402 sentence denotes The hexameric helicases, to which minichromosome maintenance (Mcm) helicase belongs, are known to assemble a ring-like structure (3–5).
T804 267-270 DT denotes The
T806 271-280 JJ denotes hexameric
T805 281-290 NNS denotes helicases
T808 290-292 , denotes ,
T809 292-294 IN denotes to
T811 295-300 WDT denotes which
T812 301-315 NN denotes minichromosome
T813 316-327 NN denotes maintenance
T815 328-329 -LRB- denotes (
T816 329-332 NN denotes Mcm
T817 332-333 -RRB- denotes )
T814 334-342 NN denotes helicase
T810 343-350 VBZ denotes belongs
T818 350-352 , denotes ,
T819 352-355 VBP denotes are
T807 356-361 VBN denotes known
T820 362-364 TO denotes to
T821 365-373 VB denotes assemble
T822 374-375 DT denotes a
T824 376-380 NN denotes ring
T826 380-381 HYPH denotes -
T825 381-385 JJ denotes like
T823 386-395 NN denotes structure
T827 396-397 -LRB- denotes (
T828 397-398 CD denotes 3
T829 398-399 SYM denotes –
T830 399-400 CD denotes 5
T831 400-401 -RRB- denotes )
T832 401-402 . denotes .
T833 402-672 sentence denotes Whereas most of DNA helicases of this group, including simian virus 40 large T-antigen (6), Escherichia coli DnaB (7) and Archaeal Mcm (4,8,9), are composed of a single subunit, the active eukaryotic Mcm helicases are composed of two trimeric Mcm4/6/7 proteins (10–14).
T834 403-410 IN denotes Whereas
T836 411-415 JJS denotes most
T837 416-418 IN denotes of
T838 419-422 NN denotes DNA
T839 423-432 NNS denotes helicases
T840 433-435 IN denotes of
T841 436-440 DT denotes this
T842 441-446 NN denotes group
T843 446-448 , denotes ,
T844 448-457 VBG denotes including
T845 458-464 JJ denotes simian
T846 465-470 NN denotes virus
T848 471-473 CD denotes 40
T849 474-479 JJ denotes large
T850 480-481 NN denotes T
T851 481-482 HYPH denotes -
T847 482-489 NN denotes antigen
T852 490-491 -LRB- denotes (
T853 491-492 CD denotes 6
T854 492-493 -RRB- denotes )
T855 493-495 , denotes ,
T856 495-506 NNP denotes Escherichia
T857 507-511 NNP denotes coli
T858 512-516 NN denotes DnaB
T859 517-518 -LRB- denotes (
T860 518-519 CD denotes 7
T861 519-520 -RRB- denotes )
T862 521-524 CC denotes and
T863 525-533 JJ denotes Archaeal
T864 534-537 NN denotes Mcm
T865 538-539 -LRB- denotes (
T867 539-540 CD denotes 4
T868 540-541 , denotes ,
T869 541-542 CD denotes 8
T870 542-543 , denotes ,
T866 543-544 CD denotes 9
T871 544-545 -RRB- denotes )
T872 545-547 , denotes ,
T873 547-550 VBP denotes are
T835 551-559 VBN denotes composed
T875 560-562 IN denotes of
T876 563-564 DT denotes a
T878 565-571 JJ denotes single
T877 572-579 NN denotes subunit
T879 579-581 , denotes ,
T880 581-584 DT denotes the
T882 585-591 JJ denotes active
T883 592-602 JJ denotes eukaryotic
T884 603-606 NN denotes Mcm
T881 607-616 NNS denotes helicases
T885 617-620 VBP denotes are
T874 621-629 VBN denotes composed
T886 630-632 IN denotes of
T887 633-636 CD denotes two
T889 637-645 JJ denotes trimeric
T890 646-650 NN denotes Mcm4
T891 650-651 HYPH denotes /
T892 651-652 CD denotes 6
T893 652-653 HYPH denotes /
T894 653-654 CD denotes 7
T888 655-663 NN denotes proteins
T895 664-665 -LRB- denotes (
T896 665-667 CD denotes 10
T897 667-668 SYM denotes –
T898 668-670 CD denotes 14
T899 670-671 -RRB- denotes )
T900 671-672 . denotes .
T901 672-788 sentence denotes All the six Mcm 2–7 proteins contain highly conserved DNA-dependent ATPase motifs in their central regions (15,16).
T902 673-676 PDT denotes All
T904 677-680 DT denotes the
T905 681-684 CD denotes six
T906 685-688 NN denotes Mcm
T907 689-690 CD denotes 2
T908 690-691 SYM denotes –
T909 691-692 CD denotes 7
T903 693-701 NN denotes proteins
T910 702-709 VBP denotes contain
T911 710-716 RB denotes highly
T912 717-726 VBN denotes conserved
T914 727-730 NN denotes DNA
T916 730-731 HYPH denotes -
T915 731-740 JJ denotes dependent
T917 741-747 NN denotes ATPase
T913 748-754 NNS denotes motifs
T918 755-757 IN denotes in
T919 758-763 PRP$ denotes their
T921 764-771 JJ denotes central
T920 772-779 NNS denotes regions
T922 780-781 -LRB- denotes (
T924 781-783 CD denotes 15
T925 783-784 , denotes ,
T923 784-786 CD denotes 16
T926 786-787 -RRB- denotes )
T927 787-788 . denotes .
T928 788-961 sentence denotes Among the several stable subcomplexes which MCM proteins can generate, only the Mcm4/6/7 complex has been shown to possess an intrinsic DNA helicase activity (10–14,17–18).
T929 789-794 IN denotes Among
T931 795-798 DT denotes the
T933 799-806 JJ denotes several
T934 807-813 JJ denotes stable
T932 814-826 NNS denotes subcomplexes
T935 827-832 WDT denotes which
T937 833-836 NN denotes MCM
T938 837-845 NN denotes proteins
T939 846-849 MD denotes can
T936 850-858 VB denotes generate
T940 858-860 , denotes ,
T941 860-864 RB denotes only
T943 865-868 DT denotes the
T944 869-873 NN denotes Mcm4
T945 873-874 HYPH denotes /
T946 874-875 CD denotes 6
T947 875-876 HYPH denotes /
T948 876-877 CD denotes 7
T942 878-885 NN denotes complex
T949 886-889 VBZ denotes has
T950 890-894 VBN denotes been
T930 895-900 VBN denotes shown
T951 901-903 TO denotes to
T952 904-911 VB denotes possess
T953 912-914 DT denotes an
T955 915-924 JJ denotes intrinsic
T956 925-928 NN denotes DNA
T957 929-937 NN denotes helicase
T954 938-946 NN denotes activity
T958 947-948 -LRB- denotes (
T959 948-950 CD denotes 10
T960 950-951 SYM denotes –
T961 951-953 CD denotes 14
T962 953-954 , denotes ,
T963 954-956 CD denotes 17
T964 956-957 SYM denotes –
T965 957-959 CD denotes 18
T966 959-960 -RRB- denotes )
T967 960-961 . denotes .
T968 961-1230 sentence denotes While Mcm4, Mcm6 and Mcm7 proteins make distinct contribution to its helicase activity (11–13), Mcm2 or Mcm3/5 inhibit the helicase activity of the Mcm4/6/7 complex by converting its double trimer structure into a heterotetramer or heteropentamer, respectively (3,11).
T969 962-967 IN denotes While
T971 968-972 NN denotes Mcm4
T973 972-974 , denotes ,
T974 974-978 NN denotes Mcm6
T975 979-982 CC denotes and
T976 983-987 NN denotes Mcm7
T972 988-996 NN denotes proteins
T970 997-1001 VBP denotes make
T978 1002-1010 JJ denotes distinct
T979 1011-1023 NN denotes contribution
T980 1024-1026 IN denotes to
T981 1027-1030 PRP$ denotes its
T983 1031-1039 NN denotes helicase
T982 1040-1048 NN denotes activity
T984 1049-1050 -LRB- denotes (
T985 1050-1052 CD denotes 11
T986 1052-1053 SYM denotes –
T987 1053-1055 CD denotes 13
T988 1055-1056 -RRB- denotes )
T989 1056-1058 , denotes ,
T990 1058-1062 NN denotes Mcm2
T991 1063-1065 CC denotes or
T992 1066-1070 NN denotes Mcm3
T993 1070-1071 HYPH denotes /
T994 1071-1072 CD denotes 5
T977 1073-1080 VBP denotes inhibit
T995 1081-1084 DT denotes the
T997 1085-1093 NN denotes helicase
T996 1094-1102 NN denotes activity
T998 1103-1105 IN denotes of
T999 1106-1109 DT denotes the
T1001 1110-1114 NN denotes Mcm4
T1002 1114-1115 HYPH denotes /
T1003 1115-1116 CD denotes 6
T1004 1116-1117 HYPH denotes /
T1005 1117-1118 CD denotes 7
T1000 1119-1126 NN denotes complex
T1006 1127-1129 IN denotes by
T1007 1130-1140 VBG denotes converting
T1008 1141-1144 PRP$ denotes its
T1010 1145-1151 JJ denotes double
T1011 1152-1158 NN denotes trimer
T1009 1159-1168 NN denotes structure
T1012 1169-1173 IN denotes into
T1013 1174-1175 DT denotes a
T1014 1176-1190 NN denotes heterotetramer
T1015 1191-1193 CC denotes or
T1016 1194-1208 NN denotes heteropentamer
T1017 1208-1210 , denotes ,
T1018 1210-1222 RB denotes respectively
T1019 1223-1224 -LRB- denotes (
T1021 1224-1225 CD denotes 3
T1022 1225-1226 , denotes ,
T1020 1226-1228 CD denotes 11
T1023 1228-1229 -RRB- denotes )
T1024 1229-1230 . denotes .
T1025 1230-1465 sentence denotes Chromatin immunoprecipitation assays and genetic characterization in Saccharomyces cerevisiae strongly suggested that Mcm is involved not only in initiation but also in the DNA chain elongation stage as a replicative helicase (19,20).
T1026 1231-1240 NN denotes Chromatin
T1028 1241-1260 NN denotes immunoprecipitation
T1027 1261-1267 NNS denotes assays
T1030 1268-1271 CC denotes and
T1031 1272-1279 JJ denotes genetic
T1032 1280-1296 NN denotes characterization
T1033 1297-1299 IN denotes in
T1034 1300-1313 NNP denotes Saccharomyces
T1035 1314-1324 NNP denotes cerevisiae
T1036 1325-1333 RB denotes strongly
T1029 1334-1343 VBD denotes suggested
T1037 1344-1348 IN denotes that
T1039 1349-1352 NN denotes Mcm
T1040 1353-1355 VBZ denotes is
T1038 1356-1364 VBN denotes involved
T1041 1365-1368 RB denotes not
T1043 1369-1373 RB denotes only
T1042 1374-1376 IN denotes in
T1044 1377-1387 NN denotes initiation
T1045 1388-1391 CC denotes but
T1046 1392-1396 RB denotes also
T1047 1397-1399 IN denotes in
T1048 1400-1403 DT denotes the
T1050 1404-1407 NN denotes DNA
T1052 1408-1413 NN denotes chain
T1051 1414-1424 NN denotes elongation
T1049 1425-1430 NN denotes stage
T1053 1431-1433 IN denotes as
T1054 1434-1435 DT denotes a
T1056 1436-1447 JJ denotes replicative
T1055 1448-1456 NN denotes helicase
T1057 1457-1458 -LRB- denotes (
T1059 1458-1460 CD denotes 19
T1060 1460-1461 , denotes ,
T1058 1461-1463 CD denotes 20
T1061 1463-1464 -RRB- denotes )
T1062 1464-1465 . denotes .
T1063 1465-1678 sentence denotes Consistent with this notion, the processivity of the Schizosaccharomyces pombe and mouse Mcm4/6/7 complexes is significantly stimulated on forked DNA structures and it can unwind duplex DNA of 400–500 bp (13,14).
T1064 1466-1476 JJ denotes Consistent
T1066 1477-1481 IN denotes with
T1067 1482-1486 DT denotes this
T1068 1487-1493 NN denotes notion
T1069 1493-1495 , denotes ,
T1070 1495-1498 DT denotes the
T1071 1499-1511 NN denotes processivity
T1072 1512-1514 IN denotes of
T1073 1515-1518 DT denotes the
T1075 1519-1538 NNP denotes Schizosaccharomyces
T1076 1539-1544 NNP denotes pombe
T1077 1545-1548 CC denotes and
T1078 1549-1554 NN denotes mouse
T1079 1555-1559 NN denotes Mcm4
T1080 1559-1560 HYPH denotes /
T1081 1560-1561 CD denotes 6
T1082 1561-1562 HYPH denotes /
T1083 1562-1563 CD denotes 7
T1074 1564-1573 NNS denotes complexes
T1084 1574-1576 VBZ denotes is
T1085 1577-1590 RB denotes significantly
T1065 1591-1601 VBN denotes stimulated
T1086 1602-1604 IN denotes on
T1087 1605-1611 VBN denotes forked
T1088 1612-1615 NN denotes DNA
T1089 1616-1626 NNS denotes structures
T1090 1627-1630 CC denotes and
T1091 1631-1633 PRP denotes it
T1093 1634-1637 MD denotes can
T1092 1638-1644 VB denotes unwind
T1094 1645-1651 NN denotes duplex
T1095 1652-1655 NN denotes DNA
T1096 1656-1658 IN denotes of
T1097 1659-1662 CD denotes 400
T1099 1662-1663 SYM denotes –
T1098 1663-1666 CD denotes 500
T1100 1667-1669 NN denotes bp
T1101 1670-1671 -LRB- denotes (
T1103 1671-1673 CD denotes 13
T1104 1673-1674 , denotes ,
T1102 1674-1676 CD denotes 14
T1105 1676-1677 -RRB- denotes )
T1106 1677-1678 . denotes .
T1107 1678-1832 sentence denotes Mcm4/6/7 binds to fork and bubble structures in an ATP-dependent manner, and generates a double-hexameric complex, as was shown for T-antigen (13,14,21).
T1108 1679-1683 NN denotes Mcm4
T1110 1683-1684 HYPH denotes /
T1111 1684-1685 CD denotes 6
T1112 1685-1686 HYPH denotes /
T1113 1686-1687 CD denotes 7
T1109 1688-1693 VBZ denotes binds
T1114 1694-1696 IN denotes to
T1115 1697-1701 NN denotes fork
T1117 1702-1705 CC denotes and
T1118 1706-1712 NN denotes bubble
T1116 1713-1723 NNS denotes structures
T1119 1724-1726 IN denotes in
T1120 1727-1729 DT denotes an
T1122 1730-1733 NN denotes ATP
T1124 1733-1734 HYPH denotes -
T1123 1734-1743 JJ denotes dependent
T1121 1744-1750 NN denotes manner
T1125 1750-1752 , denotes ,
T1126 1752-1755 CC denotes and
T1127 1756-1765 VBZ denotes generates
T1128 1766-1767 DT denotes a
T1130 1768-1774 JJ denotes double
T1132 1774-1775 HYPH denotes -
T1131 1775-1784 JJ denotes hexameric
T1129 1785-1792 NN denotes complex
T1133 1792-1794 , denotes ,
T1134 1794-1796 IN denotes as
T1136 1797-1800 VBD denotes was
T1135 1801-1806 VBN denotes shown
T1137 1807-1810 IN denotes for
T1138 1811-1812 NN denotes T
T1140 1812-1813 HYPH denotes -
T1139 1813-1820 NN denotes antigen
T1141 1821-1822 -LRB- denotes (
T1143 1822-1824 CD denotes 13
T1144 1824-1825 , denotes ,
T1145 1825-1827 CD denotes 14
T1146 1827-1828 , denotes ,
T1142 1828-1830 CD denotes 21
T1147 1830-1831 -RRB- denotes )
T1148 1831-1832 . denotes .
T1149 1832-2138 sentence denotes Recently, we reported that the helicase and ATP hydrolysis activities of mammalian Mcm4/6/7 are specifically activated by single-stranded DNA containing stretches of thymine residues and proposed a novel model that Mcm may play a crucial role in selection of replication origins in higher eukaryotes (13).
T1150 1833-1841 RB denotes Recently
T1152 1841-1843 , denotes ,
T1153 1843-1845 PRP denotes we
T1151 1846-1854 VBD denotes reported
T1154 1855-1859 IN denotes that
T1156 1860-1863 DT denotes the
T1158 1864-1872 NN denotes helicase
T1159 1873-1876 CC denotes and
T1160 1877-1880 NN denotes ATP
T1161 1881-1891 NN denotes hydrolysis
T1157 1892-1902 NNS denotes activities
T1162 1903-1905 IN denotes of
T1163 1906-1915 JJ denotes mammalian
T1164 1916-1920 NN denotes Mcm4
T1165 1920-1921 HYPH denotes /
T1166 1921-1922 CD denotes 6
T1167 1922-1923 HYPH denotes /
T1168 1923-1924 CD denotes 7
T1169 1925-1928 VBP denotes are
T1170 1929-1941 RB denotes specifically
T1155 1942-1951 VBN denotes activated
T1171 1952-1954 IN denotes by
T1172 1955-1961 JJ denotes single
T1174 1961-1962 HYPH denotes -
T1173 1962-1970 VBN denotes stranded
T1175 1971-1974 NN denotes DNA
T1176 1975-1985 VBG denotes containing
T1177 1986-1995 NNS denotes stretches
T1178 1996-1998 IN denotes of
T1179 1999-2006 NN denotes thymine
T1180 2007-2015 NNS denotes residues
T1181 2016-2019 CC denotes and
T1182 2020-2028 VBD denotes proposed
T1183 2029-2030 DT denotes a
T1185 2031-2036 JJ denotes novel
T1184 2037-2042 NN denotes model
T1186 2043-2047 IN denotes that
T1188 2048-2051 NN denotes Mcm
T1189 2052-2055 MD denotes may
T1187 2056-2060 VB denotes play
T1190 2061-2062 DT denotes a
T1192 2063-2070 JJ denotes crucial
T1191 2071-2075 NN denotes role
T1193 2076-2078 IN denotes in
T1194 2079-2088 NN denotes selection
T1195 2089-2091 IN denotes of
T1196 2092-2103 NN denotes replication
T1197 2104-2111 NNS denotes origins
T1198 2112-2114 IN denotes in
T1199 2115-2121 JJR denotes higher
T1200 2122-2132 NNS denotes eukaryotes
T1201 2133-2134 -LRB- denotes (
T1202 2134-2136 CD denotes 13
T1203 2136-2137 -RRB- denotes )
T1204 2137-2138 . denotes .
T1205 2138-2437 sentence denotes In this report, in order to clarify the mode of action of the Mcm helicase and obtain insight into how it may function at the replication forks in vivo, we have conducted detailed analyses of helicase action and DNA binding of mouse Mcm4/6/7 helicase using various forked and bubble substrate DNAs.
T1206 2139-2141 IN denotes In
T1208 2142-2146 DT denotes this
T1209 2147-2153 NN denotes report
T1210 2153-2155 , denotes ,
T1211 2155-2157 IN denotes in
T1212 2158-2163 NN denotes order
T1213 2164-2166 TO denotes to
T1214 2167-2174 VB denotes clarify
T1215 2175-2178 DT denotes the
T1216 2179-2183 NN denotes mode
T1217 2184-2186 IN denotes of
T1218 2187-2193 NN denotes action
T1219 2194-2196 IN denotes of
T1220 2197-2200 DT denotes the
T1222 2201-2204 NN denotes Mcm
T1221 2205-2213 NN denotes helicase
T1223 2214-2217 CC denotes and
T1224 2218-2224 VB denotes obtain
T1225 2225-2232 NN denotes insight
T1226 2233-2237 IN denotes into
T1227 2238-2241 WRB denotes how
T1229 2242-2244 PRP denotes it
T1230 2245-2248 MD denotes may
T1228 2249-2257 VB denotes function
T1231 2258-2260 IN denotes at
T1232 2261-2264 DT denotes the
T1234 2265-2276 NN denotes replication
T1233 2277-2282 NNS denotes forks
T1235 2283-2285 FW denotes in
T1236 2286-2290 FW denotes vivo
T1237 2290-2292 , denotes ,
T1238 2292-2294 PRP denotes we
T1239 2295-2299 VBP denotes have
T1207 2300-2309 VBN denotes conducted
T1240 2310-2318 JJ denotes detailed
T1241 2319-2327 NNS denotes analyses
T1242 2328-2330 IN denotes of
T1243 2331-2339 NN denotes helicase
T1244 2340-2346 NN denotes action
T1245 2347-2350 CC denotes and
T1246 2351-2354 NN denotes DNA
T1247 2355-2362 NN denotes binding
T1248 2363-2365 IN denotes of
T1249 2366-2371 NN denotes mouse
T1251 2372-2376 NN denotes Mcm4
T1252 2376-2377 HYPH denotes /
T1253 2377-2378 CD denotes 6
T1254 2378-2379 HYPH denotes /
T1255 2379-2380 CD denotes 7
T1250 2381-2389 NN denotes helicase
T1256 2390-2395 VBG denotes using
T1257 2396-2403 JJ denotes various
T1259 2404-2410 VBN denotes forked
T1260 2411-2414 CC denotes and
T1261 2415-2421 NN denotes bubble
T1262 2422-2431 NN denotes substrate
T1258 2432-2436 NNS denotes DNAs
T1263 2436-2437 . denotes .
T1264 2437-2676 sentence denotes We specifically addressed sequence requirement for the Mcm helicase activation, mode of interaction with DNA substrates and whether the thymine sequences are required for continuous activation of Mcm helicase during the unwinding process.
T1265 2438-2440 PRP denotes We
T1267 2441-2453 RB denotes specifically
T1266 2454-2463 VBD denotes addressed
T1268 2464-2472 NN denotes sequence
T1269 2473-2484 NN denotes requirement
T1270 2485-2488 IN denotes for
T1271 2489-2492 DT denotes the
T1273 2493-2496 NN denotes Mcm
T1274 2497-2505 NN denotes helicase
T1272 2506-2516 NN denotes activation
T1275 2516-2518 , denotes ,
T1276 2518-2522 NN denotes mode
T1277 2523-2525 IN denotes of
T1278 2526-2537 NN denotes interaction
T1279 2538-2542 IN denotes with
T1280 2543-2546 NN denotes DNA
T1281 2547-2557 NNS denotes substrates
T1282 2558-2561 CC denotes and
T1283 2562-2569 IN denotes whether
T1285 2570-2573 DT denotes the
T1287 2574-2581 NN denotes thymine
T1286 2582-2591 NNS denotes sequences
T1288 2592-2595 VBP denotes are
T1284 2596-2604 VBN denotes required
T1289 2605-2608 IN denotes for
T1290 2609-2619 JJ denotes continuous
T1291 2620-2630 NN denotes activation
T1292 2631-2633 IN denotes of
T1293 2634-2637 NN denotes Mcm
T1294 2638-2646 NN denotes helicase
T1295 2647-2653 IN denotes during
T1296 2654-2657 DT denotes the
T1298 2658-2667 JJ denotes unwinding
T1297 2668-2675 NN denotes process
T1299 2675-2676 . denotes .
T1300 2676-2877 sentence denotes The results indicate that mammalian Mcm4/6/7 primarily binds to single-stranded DNA region, and that the extent of helicase activation is related to the thymine content of the single-stranded segment.
T1301 2677-2680 DT denotes The
T1302 2681-2688 NNS denotes results
T1303 2689-2697 VBP denotes indicate
T1304 2698-2702 IN denotes that
T1306 2703-2712 JJ denotes mammalian
T1307 2713-2717 NN denotes Mcm4
T1308 2717-2718 HYPH denotes /
T1309 2718-2719 CD denotes 6
T1310 2719-2720 HYPH denotes /
T1311 2720-2721 CD denotes 7
T1312 2722-2731 RB denotes primarily
T1305 2732-2737 VBZ denotes binds
T1313 2738-2740 IN denotes to
T1314 2741-2747 JJ denotes single
T1316 2747-2748 HYPH denotes -
T1315 2748-2756 VBN denotes stranded
T1318 2757-2760 NN denotes DNA
T1317 2761-2767 NN denotes region
T1319 2767-2769 , denotes ,
T1320 2769-2772 CC denotes and
T1321 2773-2777 IN denotes that
T1323 2778-2781 DT denotes the
T1324 2782-2788 NN denotes extent
T1325 2789-2791 IN denotes of
T1326 2792-2800 NN denotes helicase
T1327 2801-2811 NN denotes activation
T1328 2812-2814 VBZ denotes is
T1322 2815-2822 VBN denotes related
T1329 2823-2825 IN denotes to
T1330 2826-2829 DT denotes the
T1332 2830-2837 NN denotes thymine
T1331 2838-2845 NN denotes content
T1333 2846-2848 IN denotes of
T1334 2849-2852 DT denotes the
T1336 2853-2859 JJ denotes single
T1338 2859-2860 HYPH denotes -
T1337 2860-2868 VBN denotes stranded
T1335 2869-2876 NN denotes segment
T1339 2876-2877 . denotes .
T1340 2877-3082 sentence denotes Unexpectedly, Mcm4/6/7 helicase is not capable of efficiently unwinding the GC-rich duplex segment, suggesting that some other mechanism may be required for completion of replication of the entire genome.
T1341 2878-2890 RB denotes Unexpectedly
T1343 2890-2892 , denotes ,
T1344 2892-2896 NN denotes Mcm4
T1346 2896-2897 HYPH denotes /
T1347 2897-2898 CD denotes 6
T1348 2898-2899 HYPH denotes /
T1349 2899-2900 CD denotes 7
T1345 2901-2909 NN denotes helicase
T1342 2910-2912 VBZ denotes is
T1350 2913-2916 RB denotes not
T1351 2917-2924 JJ denotes capable
T1352 2925-2927 IN denotes of
T1353 2928-2939 RB denotes efficiently
T1354 2940-2949 VBG denotes unwinding
T1355 2950-2953 DT denotes the
T1357 2954-2956 NN denotes GC
T1359 2956-2957 HYPH denotes -
T1358 2957-2961 JJ denotes rich
T1360 2962-2968 NN denotes duplex
T1356 2969-2976 NN denotes segment
T1361 2976-2978 , denotes ,
T1362 2978-2988 VBG denotes suggesting
T1363 2989-2993 IN denotes that
T1365 2994-2998 DT denotes some
T1367 2999-3004 JJ denotes other
T1366 3005-3014 NN denotes mechanism
T1368 3015-3018 MD denotes may
T1369 3019-3021 VB denotes be
T1364 3022-3030 VBN denotes required
T1370 3031-3034 IN denotes for
T1371 3035-3045 NN denotes completion
T1372 3046-3048 IN denotes of
T1373 3049-3060 NN denotes replication
T1374 3061-3063 IN denotes of
T1375 3064-3067 DT denotes the
T1377 3068-3074 JJ denotes entire
T1376 3075-3081 NN denotes genome
T1378 3081-3082 . denotes .
T1448 3107-3115 NNS denotes Reagents
T1449 3115-3203 sentence denotes Labeled and unlabeled dNTPs/rNTPs and Sequenase were purchased from Amersham Pharmacia.
T1450 3116-3123 VBN denotes Labeled
T1452 3124-3127 CC denotes and
T1453 3128-3137 JJ denotes unlabeled
T1454 3138-3143 NNS denotes dNTPs
T1455 3143-3144 HYPH denotes /
T1451 3144-3149 NNS denotes rNTPs
T1457 3150-3153 CC denotes and
T1458 3154-3163 NN denotes Sequenase
T1459 3164-3168 VBD denotes were
T1456 3169-3178 VBN denotes purchased
T1460 3179-3183 IN denotes from
T1461 3184-3192 NNP denotes Amersham
T1462 3193-3202 NNP denotes Pharmacia
T1463 3202-3203 . denotes .
T1464 3203-3430 sentence denotes M13mp18 and M13mp19 single-stranded circular DNA, T4 polynucleotide kinase, DNase I and the Klenow fragment were from TAKARA, Nuclease P1 was from Roche, and anti-FLAG M2 Ab (antibody)-agarose and FLAG peptide were from Sigma.
T1465 3204-3211 NN denotes M13mp18
T1467 3212-3215 CC denotes and
T1468 3216-3223 NN denotes M13mp19
T1469 3224-3230 JJ denotes single
T1471 3230-3231 HYPH denotes -
T1470 3231-3239 VBN denotes stranded
T1472 3240-3248 JJ denotes circular
T1466 3249-3252 NN denotes DNA
T1474 3252-3254 , denotes ,
T1475 3254-3256 NN denotes T4
T1477 3257-3271 NN denotes polynucleotide
T1476 3272-3278 NN denotes kinase
T1478 3278-3280 , denotes ,
T1479 3280-3285 NN denotes DNase
T1480 3286-3287 CD denotes I
T1481 3288-3291 CC denotes and
T1482 3292-3295 DT denotes the
T1484 3296-3302 NN denotes Klenow
T1483 3303-3311 NN denotes fragment
T1473 3312-3316 VBD denotes were
T1485 3317-3321 IN denotes from
T1486 3322-3328 NN denotes TAKARA
T1487 3328-3330 , denotes ,
T1488 3330-3338 NN denotes Nuclease
T1489 3339-3341 NN denotes P1
T1490 3342-3345 VBD denotes was
T1491 3346-3350 IN denotes from
T1492 3351-3356 NNP denotes Roche
T1493 3356-3358 , denotes ,
T1494 3358-3361 CC denotes and
T1495 3362-3371 JJ denotes anti-FLAG
T1497 3372-3374 NN denotes M2
T1498 3375-3377 NN denotes Ab
T1500 3378-3379 -LRB- denotes (
T1499 3379-3387 NN denotes antibody
T1501 3387-3388 -RRB- denotes )
T1502 3388-3389 HYPH denotes -
T1496 3389-3396 NN denotes agarose
T1504 3397-3400 CC denotes and
T1505 3401-3405 NN denotes FLAG
T1506 3406-3413 NN denotes peptide
T1503 3414-3418 VBD denotes were
T1507 3419-3423 IN denotes from
T1508 3424-3429 NNP denotes Sigma
T1509 3429-3430 . denotes .
T1510 3430-3515 sentence denotes SV40 T-antigen was purified from baculovirus-infected insect cells as reported (22).
T1511 3431-3435 NN denotes SV40
T1513 3436-3437 NN denotes T
T1514 3437-3438 HYPH denotes -
T1512 3438-3445 NN denotes antigen
T1516 3446-3449 VBD denotes was
T1515 3450-3458 VBN denotes purified
T1517 3459-3463 IN denotes from
T1518 3464-3475 NN denotes baculovirus
T1520 3475-3476 HYPH denotes -
T1519 3476-3484 VBN denotes infected
T1522 3485-3491 NN denotes insect
T1521 3492-3497 NNS denotes cells
T1523 3498-3500 IN denotes as
T1524 3501-3509 VBN denotes reported
T1525 3510-3511 -LRB- denotes (
T1526 3511-3513 CD denotes 22
T1527 3513-3514 -RRB- denotes )
T1528 3514-3515 . denotes .
T1529 3515-3574 sentence denotes PriA helicase protein was provided by Dr Taku Tanaka (23).
T1530 3516-3520 NN denotes PriA
T1531 3521-3529 NN denotes helicase
T1532 3530-3537 NN denotes protein
T1534 3538-3541 VBD denotes was
T1533 3542-3550 VBN denotes provided
T1535 3551-3553 IN denotes by
T1536 3554-3556 NNP denotes Dr
T1538 3557-3561 NNP denotes Taku
T1537 3562-3568 NNP denotes Tanaka
T1539 3569-3570 -LRB- denotes (
T1540 3570-3572 CD denotes 23
T1541 3572-3573 -RRB- denotes )
T1542 3573-3574 . denotes .
T1543 3574-3776 sentence denotes Oligonucleotides were purchased from Hokkaido system science Co., Ltd (Hokkaido, Japan), and were purified on polyacrylamide gel, followed by purification by Sep-Pak Plus C18 cartridge column (Waters).
T1544 3575-3591 NNS denotes Oligonucleotides
T1546 3592-3596 VBD denotes were
T1545 3597-3606 VBN denotes purchased
T1547 3607-3611 IN denotes from
T1548 3612-3620 NNP denotes Hokkaido
T1550 3621-3627 NNP denotes system
T1551 3628-3635 NNP denotes science
T1552 3636-3639 NNP denotes Co.
T1553 3639-3641 , denotes ,
T1549 3641-3644 NNP denotes Ltd
T1554 3645-3646 -LRB- denotes (
T1555 3646-3654 NNP denotes Hokkaido
T1556 3654-3656 , denotes ,
T1557 3656-3661 NNP denotes Japan
T1558 3661-3662 -RRB- denotes )
T1559 3662-3664 , denotes ,
T1560 3664-3667 CC denotes and
T1561 3668-3672 VBD denotes were
T1562 3673-3681 VBN denotes purified
T1563 3682-3684 IN denotes on
T1564 3685-3699 NN denotes polyacrylamide
T1565 3700-3703 NN denotes gel
T1566 3703-3705 , denotes ,
T1567 3705-3713 VBN denotes followed
T1568 3714-3716 IN denotes by
T1569 3717-3729 NN denotes purification
T1570 3730-3732 IN denotes by
T1571 3733-3736 NN denotes Sep
T1573 3736-3737 HYPH denotes -
T1574 3737-3740 NN denotes Pak
T1572 3741-3745 NN denotes Plus
T1576 3746-3749 NN denotes C18
T1575 3750-3759 NN denotes cartridge
T1577 3760-3766 NN denotes column
T1578 3767-3768 -LRB- denotes (
T1579 3768-3774 NNP denotes Waters
T1580 3774-3775 -RRB- denotes )
T1581 3775-3776 . denotes .
T1667 3778-3788 NN denotes Expression
T1668 3789-3792 CC denotes and
T1669 3793-3805 NN denotes purification
T1670 3806-3808 IN denotes of
T1671 3809-3814 NN denotes mouse
T1673 3815-3819 NN denotes Mcm4
T1674 3819-3820 HYPH denotes /
T1675 3820-3821 CD denotes 6
T1676 3821-3822 HYPH denotes /
T1677 3822-3823 CD denotes 7
T1672 3824-3831 NN denotes complex
T1678 3831-3941 sentence denotes The recombinant baculoviruses expressing His6-Mcm4/Mcm6 or Mcm7-FLAG proteins were previously described (11).
T1679 3832-3835 DT denotes The
T1681 3836-3847 JJ denotes recombinant
T1680 3848-3861 NNS denotes baculoviruses
T1683 3862-3872 VBG denotes expressing
T1684 3873-3877 NN denotes His6
T1686 3877-3878 HYPH denotes -
T1687 3878-3882 NN denotes Mcm4
T1688 3882-3883 HYPH denotes /
T1685 3883-3887 NN denotes Mcm6
T1690 3888-3890 CC denotes or
T1691 3891-3895 NN denotes Mcm7
T1693 3895-3896 HYPH denotes -
T1692 3896-3900 NN denotes FLAG
T1689 3901-3909 NN denotes proteins
T1694 3910-3914 VBD denotes were
T1695 3915-3925 RB denotes previously
T1682 3926-3935 VBN denotes described
T1696 3936-3937 -LRB- denotes (
T1697 3937-3939 CD denotes 11
T1698 3939-3940 -RRB- denotes )
T1699 3940-3941 . denotes .
T1700 3941-4093 sentence denotes Sf9 and High five insect cells were cultured at 27°C in Sf-900 II SFM (Life Technologies, Inc.) and EX-CELL 405 medium (JRH Biosciences), respectively.
T1701 3942-3945 NN denotes Sf9
T1703 3946-3949 CC denotes and
T1704 3950-3954 JJ denotes High
T1705 3955-3959 CD denotes five
T1706 3960-3966 NN denotes insect
T1702 3967-3972 NNS denotes cells
T1708 3973-3977 VBD denotes were
T1707 3978-3986 VBN denotes cultured
T1709 3987-3989 IN denotes at
T1710 3990-3992 CD denotes 27
T1711 3992-3994 NN denotes °C
T1712 3995-3997 IN denotes in
T1713 3998-4000 NN denotes Sf
T1715 4000-4001 HYPH denotes -
T1716 4001-4004 CD denotes 900
T1717 4005-4007 CD denotes II
T1714 4008-4011 NN denotes SFM
T1718 4012-4013 -LRB- denotes (
T1720 4013-4017 NNP denotes Life
T1721 4018-4030 NNP denotes Technologies
T1722 4030-4032 , denotes ,
T1719 4032-4036 NNP denotes Inc.
T1723 4036-4037 -RRB- denotes )
T1724 4038-4041 CC denotes and
T1725 4042-4044 NN denotes EX
T1727 4044-4045 HYPH denotes -
T1726 4045-4049 NN denotes CELL
T1729 4050-4053 CD denotes 405
T1728 4054-4060 NN denotes medium
T1730 4061-4062 -LRB- denotes (
T1732 4062-4065 NNP denotes JRH
T1731 4066-4077 NNP denotes Biosciences
T1733 4077-4078 -RRB- denotes )
T1734 4078-4080 , denotes ,
T1735 4080-4092 RB denotes respectively
T1736 4092-4093 . denotes .
T1737 4093-4315 sentence denotes For expression of the Mcm4/6/7 proteins, High five cells were coinfected with recombinant baculoviruses expressing the His6-Mcm4/Mcm6 proteins and those expressing the Mcm7-FLAG, and were collected at 48 h post-infection.
T1738 4094-4097 IN denotes For
T1740 4098-4108 NN denotes expression
T1741 4109-4111 IN denotes of
T1742 4112-4115 DT denotes the
T1744 4116-4120 NN denotes Mcm4
T1745 4120-4121 HYPH denotes /
T1746 4121-4122 CD denotes 6
T1747 4122-4123 HYPH denotes /
T1748 4123-4124 CD denotes 7
T1743 4125-4133 NN denotes proteins
T1749 4133-4135 , denotes ,
T1750 4135-4139 JJ denotes High
T1751 4140-4144 CD denotes five
T1752 4145-4150 NNS denotes cells
T1753 4151-4155 VBD denotes were
T1739 4156-4166 VBN denotes coinfected
T1754 4167-4171 IN denotes with
T1755 4172-4183 JJ denotes recombinant
T1756 4184-4197 NNS denotes baculoviruses
T1757 4198-4208 VBG denotes expressing
T1758 4209-4212 DT denotes the
T1760 4213-4217 NN denotes His6
T1762 4217-4218 HYPH denotes -
T1763 4218-4222 NN denotes Mcm4
T1764 4222-4223 HYPH denotes /
T1761 4223-4227 NN denotes Mcm6
T1759 4228-4236 NN denotes proteins
T1765 4237-4240 CC denotes and
T1766 4241-4246 DT denotes those
T1767 4247-4257 VBG denotes expressing
T1768 4258-4261 DT denotes the
T1770 4262-4266 NN denotes Mcm7
T1771 4266-4267 HYPH denotes -
T1769 4267-4271 NN denotes FLAG
T1772 4271-4273 , denotes ,
T1773 4273-4276 CC denotes and
T1774 4277-4281 VBD denotes were
T1775 4282-4291 VBN denotes collected
T1776 4292-4294 IN denotes at
T1777 4295-4297 CD denotes 48
T1778 4298-4299 NN denotes h
T1779 4300-4314 RB denotes post-infection
T1780 4314-4315 . denotes .
T1781 4315-4394 sentence denotes The recombinant Mcm4/6/7 complexes were purified as previously described (12).
T1782 4316-4319 DT denotes The
T1784 4320-4331 JJ denotes recombinant
T1785 4332-4336 NN denotes Mcm4
T1786 4336-4337 HYPH denotes /
T1787 4337-4338 CD denotes 6
T1788 4338-4339 HYPH denotes /
T1789 4339-4340 CD denotes 7
T1783 4341-4350 NNS denotes complexes
T1791 4351-4355 VBD denotes were
T1790 4356-4364 VBN denotes purified
T1792 4365-4367 IN denotes as
T1794 4368-4378 RB denotes previously
T1793 4379-4388 VBN denotes described
T1795 4389-4390 -LRB- denotes (
T1796 4390-4392 CD denotes 12
T1797 4392-4393 -RRB- denotes )
T1798 4393-4394 . denotes .
T1980 4396-4399 NN denotes DNA
T1981 4400-4410 NNS denotes substrates
T1982 4410-4524 sentence denotes The sequences for all the oligonucleotides used for constructions of DNA substrates are listed in Tables 1 and 2.
T1983 4411-4414 DT denotes The
T1984 4415-4424 NNS denotes sequences
T1986 4425-4428 IN denotes for
T1987 4429-4432 PDT denotes all
T1989 4433-4436 DT denotes the
T1988 4437-4453 NNS denotes oligonucleotides
T1990 4454-4458 VBN denotes used
T1991 4459-4462 IN denotes for
T1992 4463-4476 NNS denotes constructions
T1993 4477-4479 IN denotes of
T1994 4480-4483 NN denotes DNA
T1995 4484-4494 NNS denotes substrates
T1996 4495-4498 VBP denotes are
T1985 4499-4505 VBN denotes listed
T1997 4506-4508 IN denotes in
T1998 4509-4515 NNS denotes Tables
T1999 4516-4517 CD denotes 1
T2000 4518-4521 CC denotes and
T2001 4522-4523 CD denotes 2
T2002 4523-4524 . denotes .
T2003 4524-4676 sentence denotes The partial heteroduplex substrates were constructed by annealing labeled oligonucleotides with M13mp18 single-stranded circular DNA or its derivative.
T2004 4525-4528 DT denotes The
T2006 4529-4536 JJ denotes partial
T2007 4537-4549 NN denotes heteroduplex
T2005 4550-4560 NNS denotes substrates
T2009 4561-4565 VBD denotes were
T2008 4566-4577 VBN denotes constructed
T2010 4578-4580 IN denotes by
T2011 4581-4590 VBG denotes annealing
T2012 4591-4598 VBN denotes labeled
T2013 4599-4615 NNS denotes oligonucleotides
T2014 4616-4620 IN denotes with
T2015 4621-4628 NN denotes M13mp18
T2017 4629-4635 JJ denotes single
T2019 4635-4636 HYPH denotes -
T2018 4636-4644 VBN denotes stranded
T2020 4645-4653 JJ denotes circular
T2016 4654-4657 NN denotes DNA
T2021 4658-4660 CC denotes or
T2022 4661-4664 PRP$ denotes its
T2023 4665-4675 NN denotes derivative
T2024 4675-4676 . denotes .
T2025 4676-4783 sentence denotes The labeled substrates were purified by Sepharose CL4B column chromatography (Amersham Pharmacia Biotech).
T2026 4677-4680 DT denotes The
T2028 4681-4688 VBN denotes labeled
T2027 4689-4699 NNS denotes substrates
T2030 4700-4704 VBD denotes were
T2029 4705-4713 VBN denotes purified
T2031 4714-4716 IN denotes by
T2032 4717-4726 NN denotes Sepharose
T2033 4727-4731 NN denotes CL4B
T2035 4732-4738 NN denotes column
T2034 4739-4753 NN denotes chromatography
T2036 4754-4755 -LRB- denotes (
T2038 4755-4763 NNP denotes Amersham
T2039 4764-4773 NNP denotes Pharmacia
T2037 4774-4781 NNP denotes Biotech
T2040 4781-4782 -RRB- denotes )
T2041 4782-4783 . denotes .
T2042 4783-4922 sentence denotes The DNA bubble substrates were assembled from two partially complementary oligonucleotides with top and bottom strand sequences (Table 1).
T2043 4784-4787 DT denotes The
T2045 4788-4791 NN denotes DNA
T2046 4792-4798 NN denotes bubble
T2044 4799-4809 NNS denotes substrates
T2048 4810-4814 VBD denotes were
T2047 4815-4824 VBN denotes assembled
T2049 4825-4829 IN denotes from
T2050 4830-4833 CD denotes two
T2052 4834-4843 RB denotes partially
T2053 4844-4857 JJ denotes complementary
T2051 4858-4874 NNS denotes oligonucleotides
T2054 4875-4879 IN denotes with
T2055 4880-4883 JJ denotes top
T2057 4884-4887 CC denotes and
T2058 4888-4894 JJ denotes bottom
T2059 4895-4901 NN denotes strand
T2056 4902-4911 NNS denotes sequences
T2060 4912-4913 -LRB- denotes (
T2061 4913-4918 NN denotes Table
T2062 4919-4920 CD denotes 1
T2063 4920-4921 -RRB- denotes )
T2064 4921-4922 . denotes .
T2065 4922-5021 sentence denotes T-tailed Y-fork substrates were prepared by annealing two oligonucleotides (dT30 and dT60 series).
T2066 4923-4924 NN denotes T
T2068 4924-4925 HYPH denotes -
T2067 4925-4931 VBN denotes tailed
T2070 4932-4933 NN denotes Y
T2072 4933-4934 HYPH denotes -
T2071 4934-4938 NN denotes fork
T2069 4939-4949 NNS denotes substrates
T2074 4950-4954 VBD denotes were
T2073 4955-4963 VBN denotes prepared
T2075 4964-4966 IN denotes by
T2076 4967-4976 VBG denotes annealing
T2077 4977-4980 CD denotes two
T2078 4981-4997 NNS denotes oligonucleotides
T2079 4998-4999 -LRB- denotes (
T2081 4999-5003 NN denotes dT30
T2082 5004-5007 CC denotes and
T2083 5008-5012 NN denotes dT60
T2080 5013-5019 NNS denotes series
T2084 5019-5020 -RRB- denotes )
T2085 5020-5021 . denotes .
T2086 5021-5161 sentence denotes To construct the arrested fork and 3′- or 5′-extension substrates, various combinations of oligonucleotides shown in Table 1 were annealed.
T2087 5022-5024 TO denotes To
T2088 5025-5034 VB denotes construct
T2090 5035-5038 DT denotes the
T2092 5039-5047 VBN denotes arrested
T2091 5048-5052 NN denotes fork
T2093 5053-5056 CC denotes and
T2094 5057-5058 CD denotes 3
T2096 5058-5059 SYM denotes ′
T2097 5059-5060 HYPH denotes -
T2098 5061-5063 CC denotes or
T2099 5064-5065 CD denotes 5
T2100 5065-5066 SYM denotes ′
T2101 5066-5067 HYPH denotes -
T2102 5067-5076 NN denotes extension
T2095 5077-5087 NNS denotes substrates
T2103 5087-5089 , denotes ,
T2104 5089-5096 JJ denotes various
T2105 5097-5109 NNS denotes combinations
T2106 5110-5112 IN denotes of
T2107 5113-5129 NNS denotes oligonucleotides
T2108 5130-5135 VBN denotes shown
T2109 5136-5138 IN denotes in
T2110 5139-5144 NN denotes Table
T2111 5145-5146 CD denotes 1
T2112 5147-5151 VBD denotes were
T2089 5152-5160 VBN denotes annealed
T2113 5160-5161 . denotes .
T2114 5161-5282 sentence denotes Bub-82/lamin B2 containing the T-rich strand of the lamin B2 origin region was constructed as previously described (13).
T2115 5162-5165 NN denotes Bub
T2117 5165-5166 HYPH denotes -
T2118 5166-5168 CD denotes 82
T2119 5168-5169 HYPH denotes /
T2120 5169-5174 NN denotes lamin
T2116 5175-5177 NN denotes B2
T2122 5178-5188 VBG denotes containing
T2123 5189-5192 DT denotes the
T2125 5193-5194 NN denotes T
T2127 5194-5195 HYPH denotes -
T2126 5195-5199 JJ denotes rich
T2124 5200-5206 NN denotes strand
T2128 5207-5209 IN denotes of
T2129 5210-5213 DT denotes the
T2131 5214-5219 NN denotes lamin
T2132 5220-5222 NN denotes B2
T2133 5223-5229 NN denotes origin
T2130 5230-5236 NN denotes region
T2134 5237-5240 VBD denotes was
T2121 5241-5252 VBN denotes constructed
T2135 5253-5255 IN denotes as
T2137 5256-5266 RB denotes previously
T2136 5267-5276 VBN denotes described
T2138 5277-5278 -LRB- denotes (
T2139 5278-5280 CD denotes 13
T2140 5280-5281 -RRB- denotes )
T2141 5281-5282 . denotes .
T2142 5282-5504 sentence denotes The bubble substrate, c-myc/DUE or c-myc/DUE-C, contains sequences (GenBank accession number K01908) from nucleotides 722 to 853 or those from 828 to 747 (complementary strand), respectively, in the central melted region.
T2143 5283-5286 DT denotes The
T2145 5287-5293 NN denotes bubble
T2144 5294-5303 NN denotes substrate
T2147 5303-5305 , denotes ,
T2148 5305-5306 NN denotes c
T2150 5306-5307 HYPH denotes -
T2149 5307-5310 NN denotes myc
T2152 5310-5311 HYPH denotes /
T2151 5311-5314 NN denotes DUE
T2153 5315-5317 CC denotes or
T2154 5318-5319 NN denotes c
T2156 5319-5320 HYPH denotes -
T2155 5320-5323 NN denotes myc
T2158 5323-5324 HYPH denotes /
T2159 5324-5327 NN denotes DUE
T2160 5327-5328 HYPH denotes -
T2157 5328-5329 NN denotes C
T2161 5329-5331 , denotes ,
T2146 5331-5339 VBZ denotes contains
T2162 5340-5349 NNS denotes sequences
T2163 5350-5351 -LRB- denotes (
T2165 5351-5358 NNP denotes GenBank
T2167 5359-5368 NN denotes accession
T2166 5369-5375 NN denotes number
T2164 5376-5382 NN denotes K01908
T2168 5382-5383 -RRB- denotes )
T2169 5384-5388 IN denotes from
T2170 5389-5400 NNS denotes nucleotides
T2172 5401-5404 CD denotes 722
T2173 5405-5407 IN denotes to
T2171 5408-5411 CD denotes 853
T2174 5412-5414 CC denotes or
T2175 5415-5420 DT denotes those
T2176 5421-5425 IN denotes from
T2177 5426-5429 CD denotes 828
T2178 5430-5432 IN denotes to
T2179 5433-5436 CD denotes 747
T2180 5437-5438 -LRB- denotes (
T2182 5438-5451 JJ denotes complementary
T2181 5452-5458 NN denotes strand
T2183 5458-5459 -RRB- denotes )
T2184 5459-5461 , denotes ,
T2185 5461-5473 RB denotes respectively
T2186 5473-5475 , denotes ,
T2187 5475-5477 IN denotes in
T2188 5478-5481 DT denotes the
T2190 5482-5489 JJ denotes central
T2191 5490-5496 VBN denotes melted
T2189 5497-5503 NN denotes region
T2192 5503-5504 . denotes .
T2193 5504-5803 sentence denotes For each substrate, labeled oligonucleotide (3 pmol) was annealed with the unlabeled oligonucleotide (6 pmol) in a reaction mixture (50 μl) containing 20 mM Tris–HCl (pH 7.5), 10 mM MgCl2 and 25 mM NaCl, which were heated to 95°C, kept at 67°C for 1 h, and then allowed to slowly cool down to 37°C.
T2194 5505-5508 IN denotes For
T2196 5509-5513 DT denotes each
T2197 5514-5523 NN denotes substrate
T2198 5523-5525 , denotes ,
T2199 5525-5532 VBN denotes labeled
T2200 5533-5548 NN denotes oligonucleotide
T2201 5549-5550 -LRB- denotes (
T2203 5550-5551 CD denotes 3
T2202 5552-5556 NN denotes pmol
T2204 5556-5557 -RRB- denotes )
T2205 5558-5561 VBD denotes was
T2195 5562-5570 VBN denotes annealed
T2206 5571-5575 IN denotes with
T2207 5576-5579 DT denotes the
T2209 5580-5589 JJ denotes unlabeled
T2208 5590-5605 NN denotes oligonucleotide
T2210 5606-5607 -LRB- denotes (
T2212 5607-5608 CD denotes 6
T2211 5609-5613 NN denotes pmol
T2213 5613-5614 -RRB- denotes )
T2214 5615-5617 IN denotes in
T2215 5618-5619 DT denotes a
T2217 5620-5628 NN denotes reaction
T2216 5629-5636 NN denotes mixture
T2218 5637-5638 -LRB- denotes (
T2220 5638-5640 CD denotes 50
T2219 5641-5643 NN denotes μl
T2221 5643-5644 -RRB- denotes )
T2222 5645-5655 VBG denotes containing
T2223 5656-5658 CD denotes 20
T2224 5659-5661 NN denotes mM
T2226 5662-5666 NN denotes Tris
T2227 5666-5667 HYPH denotes –
T2225 5667-5670 NN denotes HCl
T2228 5671-5672 -LRB- denotes (
T2229 5672-5674 NN denotes pH
T2230 5675-5678 CD denotes 7.5
T2231 5678-5679 -RRB- denotes )
T2232 5679-5681 , denotes ,
T2233 5681-5683 CD denotes 10
T2234 5684-5686 NN denotes mM
T2235 5687-5692 NN denotes MgCl2
T2236 5693-5696 CC denotes and
T2237 5697-5699 CD denotes 25
T2238 5700-5702 NN denotes mM
T2239 5703-5707 NN denotes NaCl
T2240 5707-5709 , denotes ,
T2241 5709-5714 WDT denotes which
T2243 5715-5719 VBD denotes were
T2242 5720-5726 VBN denotes heated
T2244 5727-5729 IN denotes to
T2245 5730-5732 CD denotes 95
T2246 5732-5734 NN denotes °C
T2247 5734-5736 , denotes ,
T2248 5736-5740 VBD denotes kept
T2249 5741-5743 IN denotes at
T2250 5744-5746 CD denotes 67
T2251 5746-5748 NN denotes °C
T2252 5749-5752 IN denotes for
T2253 5753-5754 CD denotes 1
T2254 5755-5756 NN denotes h
T2255 5756-5758 , denotes ,
T2256 5758-5761 CC denotes and
T2257 5762-5766 RB denotes then
T2258 5767-5774 VBN denotes allowed
T2259 5775-5777 TO denotes to
T2261 5778-5784 RB denotes slowly
T2260 5785-5789 VB denotes cool
T2262 5790-5794 RP denotes down
T2263 5795-5797 IN denotes to
T2264 5798-5800 CD denotes 37
T2265 5800-5802 NN denotes °C
T2266 5802-5803 . denotes .
T2267 5803-5898 sentence denotes The assembled substrates were purified from polyacrylamide gel by elution into TE buffer (24).
T2268 5804-5807 DT denotes The
T2270 5808-5817 VBN denotes assembled
T2269 5818-5828 NNS denotes substrates
T2272 5829-5833 VBD denotes were
T2271 5834-5842 VBN denotes purified
T2273 5843-5847 IN denotes from
T2274 5848-5862 NN denotes polyacrylamide
T2275 5863-5866 NN denotes gel
T2276 5867-5869 IN denotes by
T2277 5870-5877 NN denotes elution
T2278 5878-5882 IN denotes into
T2279 5883-5885 NN denotes TE
T2280 5886-5892 NN denotes buffer
T2281 5893-5894 -LRB- denotes (
T2282 5894-5896 CD denotes 24
T2283 5896-5897 -RRB- denotes )
T2284 5897-5898 . denotes .
T2285 5898-6192 sentence denotes The partial heteroduplex substrates on a single-stranded circular DNA with varied lengths of duplex regions were prepared by extending DNA chains from the 3′ end of the dT40-37mer (37mer region hybridizing at positions 6289–6326 of M13mp18 vector) annealed to the single-stranded circular DNA.
T2286 5899-5902 DT denotes The
T2288 5903-5910 JJ denotes partial
T2289 5911-5923 NN denotes heteroduplex
T2287 5924-5934 NNS denotes substrates
T2291 5935-5937 IN denotes on
T2292 5938-5939 DT denotes a
T2294 5940-5946 JJ denotes single
T2296 5946-5947 HYPH denotes -
T2295 5947-5955 VBN denotes stranded
T2297 5956-5964 JJ denotes circular
T2293 5965-5968 NN denotes DNA
T2298 5969-5973 IN denotes with
T2299 5974-5980 VBN denotes varied
T2300 5981-5988 NNS denotes lengths
T2301 5989-5991 IN denotes of
T2302 5992-5998 NN denotes duplex
T2303 5999-6006 NNS denotes regions
T2304 6007-6011 VBD denotes were
T2290 6012-6020 VBN denotes prepared
T2305 6021-6023 IN denotes by
T2306 6024-6033 VBG denotes extending
T2307 6034-6037 NN denotes DNA
T2308 6038-6044 NNS denotes chains
T2309 6045-6049 IN denotes from
T2310 6050-6053 DT denotes the
T2312 6054-6055 CD denotes 3
T2313 6055-6056 SYM denotes ′
T2311 6057-6060 NN denotes end
T2314 6061-6063 IN denotes of
T2315 6064-6067 DT denotes the
T2317 6068-6072 NN denotes dT40
T2318 6072-6073 HYPH denotes -
T2316 6073-6078 NN denotes 37mer
T2319 6079-6080 -LRB- denotes (
T2320 6080-6085 NN denotes 37mer
T2321 6086-6092 NN denotes region
T2322 6093-6104 VBG denotes hybridizing
T2323 6105-6107 IN denotes at
T2324 6108-6117 NNS denotes positions
T2325 6118-6122 CD denotes 6289
T2326 6122-6123 SYM denotes –
T2327 6123-6127 CD denotes 6326
T2328 6128-6130 IN denotes of
T2329 6131-6138 NN denotes M13mp18
T2330 6139-6145 NN denotes vector
T2331 6145-6146 -RRB- denotes )
T2332 6147-6155 VBN denotes annealed
T2333 6156-6158 IN denotes to
T2334 6159-6162 DT denotes the
T2336 6163-6169 JJ denotes single
T2338 6169-6170 HYPH denotes -
T2337 6170-6178 VBN denotes stranded
T2339 6179-6187 JJ denotes circular
T2335 6188-6191 NN denotes DNA
T2340 6191-6192 . denotes .
T2341 6192-6413 sentence denotes DNA chains were elongated with Sequenase in the presence of [α-32P]dGTP, followed by extension after addition of ddGTP and all four dNTPs, resulting in substrates containing labeled duplex regions of varied lengths (13).
T2342 6193-6196 NN denotes DNA
T2343 6197-6203 NNS denotes chains
T2345 6204-6208 VBD denotes were
T2344 6209-6218 VBN denotes elongated
T2346 6219-6223 IN denotes with
T2347 6224-6233 NN denotes Sequenase
T2348 6234-6236 IN denotes in
T2349 6237-6240 DT denotes the
T2350 6241-6249 NN denotes presence
T2351 6250-6252 IN denotes of
T2352 6253-6254 -LRB- denotes [
T2354 6254-6255 NN denotes α
T2356 6255-6256 HYPH denotes -
T2355 6256-6259 NN denotes 32P
T2357 6259-6260 -RRB- denotes ]
T2353 6260-6264 NN denotes dGTP
T2358 6264-6266 , denotes ,
T2359 6266-6274 VBN denotes followed
T2360 6275-6277 IN denotes by
T2361 6278-6287 NN denotes extension
T2362 6288-6293 IN denotes after
T2363 6294-6302 NN denotes addition
T2364 6303-6305 IN denotes of
T2365 6306-6311 NN denotes ddGTP
T2366 6312-6315 CC denotes and
T2367 6316-6319 DT denotes all
T2369 6320-6324 CD denotes four
T2368 6325-6330 NNS denotes dNTPs
T2370 6330-6332 , denotes ,
T2371 6332-6341 VBG denotes resulting
T2372 6342-6344 IN denotes in
T2373 6345-6355 NNS denotes substrates
T2374 6356-6366 VBG denotes containing
T2375 6367-6374 VBN denotes labeled
T2377 6375-6381 NN denotes duplex
T2376 6382-6389 NNS denotes regions
T2378 6390-6392 IN denotes of
T2379 6393-6399 VBN denotes varied
T2380 6400-6407 NNS denotes lengths
T2381 6408-6409 -LRB- denotes (
T2382 6409-6411 CD denotes 13
T2383 6411-6412 -RRB- denotes )
T2384 6412-6413 . denotes .
T2385 6413-6687 sentence denotes The substrate (5 fmol) was first incubated with indicated amount of the Mcm4/6/7 complex at 37°C for 1 h in reaction mixture as described (11), and then reactions were stopped by addition of EDTA (20 mM), SDS (0.1%) and 2 μg proteinase K, followed by incubation for 20 min.
T2386 6414-6417 DT denotes The
T2387 6418-6427 NN denotes substrate
T2389 6428-6429 -LRB- denotes (
T2391 6429-6430 CD denotes 5
T2390 6431-6435 NN denotes fmol
T2392 6435-6436 -RRB- denotes )
T2393 6437-6440 VBD denotes was
T2394 6441-6446 RB denotes first
T2388 6447-6456 VBN denotes incubated
T2395 6457-6461 IN denotes with
T2396 6462-6471 VBN denotes indicated
T2397 6472-6478 NN denotes amount
T2398 6479-6481 IN denotes of
T2399 6482-6485 DT denotes the
T2401 6486-6490 NN denotes Mcm4
T2402 6490-6491 HYPH denotes /
T2403 6491-6492 CD denotes 6
T2404 6492-6493 HYPH denotes /
T2405 6493-6494 CD denotes 7
T2400 6495-6502 NN denotes complex
T2406 6503-6505 IN denotes at
T2407 6506-6508 CD denotes 37
T2408 6508-6510 NN denotes °C
T2409 6511-6514 IN denotes for
T2410 6515-6516 CD denotes 1
T2411 6517-6518 NN denotes h
T2412 6519-6521 IN denotes in
T2413 6522-6530 NN denotes reaction
T2414 6531-6538 NN denotes mixture
T2415 6539-6541 IN denotes as
T2416 6542-6551 VBN denotes described
T2417 6552-6553 -LRB- denotes (
T2418 6553-6555 CD denotes 11
T2419 6555-6556 -RRB- denotes )
T2420 6556-6558 , denotes ,
T2421 6558-6561 CC denotes and
T2422 6562-6566 RB denotes then
T2423 6567-6576 NNS denotes reactions
T2425 6577-6581 VBD denotes were
T2424 6582-6589 VBN denotes stopped
T2426 6590-6592 IN denotes by
T2427 6593-6601 NN denotes addition
T2428 6602-6604 IN denotes of
T2429 6605-6609 NN denotes EDTA
T2430 6610-6611 -LRB- denotes (
T2432 6611-6613 CD denotes 20
T2431 6614-6616 NN denotes mM
T2433 6616-6617 -RRB- denotes )
T2434 6617-6619 , denotes ,
T2435 6619-6622 NN denotes SDS
T2436 6623-6624 -LRB- denotes (
T2438 6624-6627 CD denotes 0.1
T2437 6627-6628 NN denotes %
T2439 6628-6629 -RRB- denotes )
T2440 6630-6633 CC denotes and
T2441 6634-6635 CD denotes 2
T2442 6636-6638 NN denotes μg
T2444 6639-6649 NN denotes proteinase
T2443 6650-6651 NN denotes K
T2445 6651-6653 , denotes ,
T2446 6653-6661 VBN denotes followed
T2447 6662-6664 IN denotes by
T2448 6665-6675 NN denotes incubation
T2449 6676-6679 IN denotes for
T2450 6680-6682 CD denotes 20
T2451 6683-6686 NN denotes min
T2452 6686-6687 . denotes .
T2453 6687-6776 sentence denotes Aliquots were electrophoresed on a 6.5% polyacrylamide gel in 1× TBE at 300 V for 2.5 h.
T2454 6688-6696 NNS denotes Aliquots
T2456 6697-6701 VBD denotes were
T2455 6702-6717 VBN denotes electrophoresed
T2457 6718-6720 IN denotes on
T2458 6721-6722 DT denotes a
T2460 6723-6726 CD denotes 6.5
T2461 6726-6727 NN denotes %
T2462 6728-6742 NN denotes polyacrylamide
T2459 6743-6746 NN denotes gel
T2463 6747-6749 IN denotes in
T2464 6750-6751 CD denotes 1
T2466 6751-6752 SYM denotes ×
T2465 6753-6756 NN denotes TBE
T2467 6757-6759 IN denotes at
T2468 6760-6763 CD denotes 300
T2469 6764-6765 NN denotes V
T2470 6766-6769 IN denotes for
T2471 6770-6773 CD denotes 2.5
T2472 6774-6775 NN denotes h
T2473 6775-6776 . denotes .
T2474 6776-6848 sentence denotes The single-stranded circular DNAs used were M13mp18 and M13mp19+G-rich.
T2475 6777-6780 DT denotes The
T2477 6781-6787 JJ denotes single
T2479 6787-6788 HYPH denotes -
T2478 6788-6796 VBN denotes stranded
T2480 6797-6805 JJ denotes circular
T2476 6806-6810 NNS denotes DNAs
T2482 6811-6815 VBN denotes used
T2481 6816-6820 VBD denotes were
T2483 6821-6828 NN denotes M13mp18
T2484 6829-6832 CC denotes and
T2485 6833-6840 NN denotes M13mp19
T2486 6840-6841 SYM denotes +
T2487 6841-6842 NN denotes G
T2488 6842-6843 HYPH denotes -
T2489 6843-6847 JJ denotes rich
T2490 6847-6848 . denotes .
T2491 6848-7108 sentence denotes The latter was constructed by inserting a G-rich repeat sequence (GGGGCGTGGGC)6, prepared by annealing of two oligonucleotides (5′-GATCC-[GGGGCGTGGGC]6-3′/5′-GATCC-[GCCCACGCCCC]6-3′) at the BamHI site of M13mp19 and the sequences of the insert were confirmed.
T2492 6849-6852 DT denotes The
T2493 6853-6859 JJ denotes latter
T2495 6860-6863 VBD denotes was
T2494 6864-6875 VBN denotes constructed
T2496 6876-6878 IN denotes by
T2497 6879-6888 VBG denotes inserting
T2498 6889-6890 DT denotes a
T2500 6891-6892 NN denotes G
T2501 6892-6893 HYPH denotes -
T2502 6893-6897 JJ denotes rich
T2503 6898-6904 NN denotes repeat
T2499 6905-6913 NN denotes sequence
T2504 6914-6915 -LRB- denotes (
T2505 6915-6926 NN denotes GGGGCGTGGGC
T2506 6926-6927 -RRB- denotes )
T2507 6927-6928 CD denotes 6
T2508 6928-6930 , denotes ,
T2509 6930-6938 VBN denotes prepared
T2510 6939-6941 IN denotes by
T2511 6942-6951 VBG denotes annealing
T2512 6952-6954 IN denotes of
T2513 6955-6958 CD denotes two
T2514 6959-6975 NNS denotes oligonucleotides
T2515 6976-6977 -LRB- denotes (
T2516 6977-6978 CD denotes 5
T2517 6978-6979 SYM denotes ′
T2518 6979-6980 HYPH denotes -
T2519 6980-6985 NN denotes GATCC
T2521 6985-6986 HYPH denotes -
T2522 6986-6987 -LRB- denotes [
T2520 6987-6998 NN denotes GGGGCGTGGGC
T2523 6998-6999 -RRB- denotes ]
T2524 6999-7000 CD denotes 6
T2525 7000-7001 HYPH denotes -
T2526 7001-7002 CD denotes 3
T2527 7002-7003 SYM denotes ′
T2528 7003-7004 HYPH denotes /
T2529 7004-7005 CD denotes 5
T2530 7005-7006 SYM denotes ′
T2531 7006-7007 HYPH denotes -
T2532 7007-7012 NN denotes GATCC
T2534 7012-7013 HYPH denotes -
T2535 7013-7014 -LRB- denotes [
T2533 7014-7025 NN denotes GCCCACGCCCC
T2536 7025-7026 -RRB- denotes ]
T2537 7026-7027 CD denotes 6
T2538 7027-7028 HYPH denotes -
T2539 7028-7029 CD denotes 3
T2540 7029-7030 SYM denotes ′
T2541 7030-7031 -RRB- denotes )
T2542 7032-7034 IN denotes at
T2543 7035-7038 DT denotes the
T2545 7039-7044 NN denotes BamHI
T2544 7045-7049 NN denotes site
T2546 7050-7052 IN denotes of
T2547 7053-7060 NN denotes M13mp19
T2548 7061-7064 CC denotes and
T2549 7065-7068 DT denotes the
T2550 7069-7078 NNS denotes sequences
T2552 7079-7081 IN denotes of
T2553 7082-7085 DT denotes the
T2554 7086-7092 NN denotes insert
T2555 7093-7097 VBD denotes were
T2551 7098-7107 VBN denotes confirmed
T2556 7107-7108 . denotes .
T2629 7110-7113 NN denotes DNA
T2630 7114-7122 NN denotes helicase
T2632 7123-7126 CC denotes and
T2633 7127-7130 NN denotes gel
T2634 7131-7136 NN denotes shift
T2631 7137-7143 NNS denotes assays
T2635 7143-7413 sentence denotes In gel shift assays, Mcm4/6/7 proteins were incubated at 30°C for 30 min in reaction mixtures (15 μl) containing 25 mM HEPES–NaOH (pH 7.5), 50 mM sodium acetate, 10 mM Mg(CH3COO)2, 1 mM DTT, 0.25 μg/ml BSA, 0.5 mM ATP-γ-S and labeled substrates of the amount indicated.
T2636 7144-7146 IN denotes In
T2638 7147-7150 NN denotes gel
T2639 7151-7156 NN denotes shift
T2640 7157-7163 NNS denotes assays
T2641 7163-7165 , denotes ,
T2642 7165-7169 NN denotes Mcm4
T2644 7169-7170 HYPH denotes /
T2645 7170-7171 CD denotes 6
T2646 7171-7172 HYPH denotes /
T2647 7172-7173 CD denotes 7
T2643 7174-7182 NN denotes proteins
T2648 7183-7187 VBD denotes were
T2637 7188-7197 VBN denotes incubated
T2649 7198-7200 IN denotes at
T2650 7201-7203 CD denotes 30
T2651 7203-7205 NN denotes °C
T2652 7206-7209 IN denotes for
T2653 7210-7212 CD denotes 30
T2654 7213-7216 NN denotes min
T2655 7217-7219 IN denotes in
T2656 7220-7228 NN denotes reaction
T2657 7229-7237 NNS denotes mixtures
T2658 7238-7239 -LRB- denotes (
T2660 7239-7241 CD denotes 15
T2659 7242-7244 NN denotes μl
T2661 7244-7245 -RRB- denotes )
T2662 7246-7256 VBG denotes containing
T2663 7257-7259 CD denotes 25
T2664 7260-7262 NN denotes mM
T2666 7263-7268 NN denotes HEPES
T2667 7268-7269 HYPH denotes –
T2665 7269-7273 NN denotes NaOH
T2668 7274-7275 -LRB- denotes (
T2669 7275-7277 NN denotes pH
T2670 7278-7281 CD denotes 7.5
T2671 7281-7282 -RRB- denotes )
T2672 7282-7284 , denotes ,
T2673 7284-7286 CD denotes 50
T2674 7287-7289 NN denotes mM
T2676 7290-7296 NN denotes sodium
T2675 7297-7304 NN denotes acetate
T2677 7304-7306 , denotes ,
T2678 7306-7308 CD denotes 10
T2679 7309-7311 NN denotes mM
T2681 7312-7314 NN denotes Mg
T2682 7314-7315 -LRB- denotes (
T2680 7315-7321 NN denotes CH3COO
T2683 7321-7322 -RRB- denotes )
T2684 7322-7323 CD denotes 2
T2685 7323-7325 , denotes ,
T2686 7325-7326 CD denotes 1
T2687 7327-7329 NN denotes mM
T2688 7330-7333 NN denotes DTT
T2689 7333-7335 , denotes ,
T2690 7335-7339 CD denotes 0.25
T2691 7340-7342 NN denotes μg
T2693 7342-7343 SYM denotes /
T2694 7343-7345 NN denotes ml
T2692 7346-7349 NN denotes BSA
T2695 7349-7351 , denotes ,
T2696 7351-7354 CD denotes 0.5
T2697 7355-7357 NN denotes mM
T2699 7358-7361 NN denotes ATP
T2700 7361-7362 HYPH denotes -
T2701 7362-7363 SYM denotes γ
T2702 7363-7364 HYPH denotes -
T2698 7364-7365 NN denotes S
T2703 7366-7369 CC denotes and
T2704 7370-7377 VBN denotes labeled
T2705 7378-7388 NNS denotes substrates
T2706 7389-7391 IN denotes of
T2707 7392-7395 DT denotes the
T2708 7396-7402 NN denotes amount
T2709 7403-7412 VBN denotes indicated
T2710 7412-7413 . denotes .
T2711 7413-7639 sentence denotes After addition of 2 μl of 50% glycerol, the reaction mixtures were directly applied on a polyacrylamide gel containing 6 mM magnesium acetate and 5% glycerol in 0.5× TBE, and electrophoresis was conducted at room temperature.
T2712 7414-7419 IN denotes After
T2714 7420-7428 NN denotes addition
T2715 7429-7431 IN denotes of
T2716 7432-7433 CD denotes 2
T2717 7434-7436 NN denotes μl
T2718 7437-7439 IN denotes of
T2719 7440-7442 CD denotes 50
T2720 7442-7443 NN denotes %
T2721 7444-7452 NN denotes glycerol
T2722 7452-7454 , denotes ,
T2723 7454-7457 DT denotes the
T2725 7458-7466 NN denotes reaction
T2724 7467-7475 NNS denotes mixtures
T2726 7476-7480 VBD denotes were
T2727 7481-7489 RB denotes directly
T2713 7490-7497 VBN denotes applied
T2728 7498-7500 IN denotes on
T2729 7501-7502 DT denotes a
T2731 7503-7517 NN denotes polyacrylamide
T2730 7518-7521 NN denotes gel
T2732 7522-7532 VBG denotes containing
T2733 7533-7534 CD denotes 6
T2734 7535-7537 NN denotes mM
T2736 7538-7547 NN denotes magnesium
T2735 7548-7555 NN denotes acetate
T2737 7556-7559 CC denotes and
T2738 7560-7561 CD denotes 5
T2739 7561-7562 NN denotes %
T2740 7563-7571 NN denotes glycerol
T2741 7572-7574 IN denotes in
T2742 7575-7578 CD denotes 0.5
T2744 7578-7579 SYM denotes ×
T2743 7580-7583 NN denotes TBE
T2745 7583-7585 , denotes ,
T2746 7585-7588 CC denotes and
T2747 7589-7604 NN denotes electrophoresis
T2749 7605-7608 VBD denotes was
T2748 7609-7618 VBN denotes conducted
T2750 7619-7621 IN denotes at
T2751 7622-7626 NN denotes room
T2752 7627-7638 NN denotes temperature
T2753 7638-7639 . denotes .
T2754 7639-7857 sentence denotes For DNA helicase assays, the reaction mixtures of the gel shift assays, as described above, were incubated at 30°C for 15 min, and then the ATP was added at 10 mM, followed by incubation at 37°C for additional 30 min.
T2755 7640-7643 IN denotes For
T2757 7644-7647 NN denotes DNA
T2759 7648-7656 NN denotes helicase
T2758 7657-7663 NNS denotes assays
T2760 7663-7665 , denotes ,
T2761 7665-7668 DT denotes the
T2763 7669-7677 NN denotes reaction
T2762 7678-7686 NNS denotes mixtures
T2764 7687-7689 IN denotes of
T2765 7690-7693 DT denotes the
T2767 7694-7697 NN denotes gel
T2768 7698-7703 NN denotes shift
T2766 7704-7710 NNS denotes assays
T2769 7710-7712 , denotes ,
T2770 7712-7714 IN denotes as
T2771 7715-7724 VBN denotes described
T2772 7725-7730 RB denotes above
T2773 7730-7732 , denotes ,
T2774 7732-7736 VBD denotes were
T2756 7737-7746 VBN denotes incubated
T2775 7747-7749 IN denotes at
T2776 7750-7752 CD denotes 30
T2777 7752-7754 NN denotes °C
T2778 7755-7758 IN denotes for
T2779 7759-7761 CD denotes 15
T2780 7762-7765 NN denotes min
T2781 7765-7767 , denotes ,
T2782 7767-7770 CC denotes and
T2783 7771-7775 RB denotes then
T2784 7776-7779 DT denotes the
T2785 7780-7783 NN denotes ATP
T2787 7784-7787 VBD denotes was
T2786 7788-7793 VBN denotes added
T2788 7794-7796 IN denotes at
T2789 7797-7799 CD denotes 10
T2790 7800-7802 NN denotes mM
T2791 7802-7804 , denotes ,
T2792 7804-7812 VBN denotes followed
T2793 7813-7815 IN denotes by
T2794 7816-7826 NN denotes incubation
T2795 7827-7829 IN denotes at
T2796 7830-7832 CD denotes 37
T2797 7832-7834 NN denotes °C
T2798 7835-7838 IN denotes for
T2799 7839-7849 JJ denotes additional
T2801 7850-7852 CD denotes 30
T2800 7853-7856 NN denotes min
T2802 7856-7857 . denotes .
T2803 7857-7992 sentence denotes The reactions were terminated by addition of EDTA (20 mM), SDS (0.1%) and 2 μg proteinase K, and were incubated for additional 15 min.
T2804 7858-7861 DT denotes The
T2805 7862-7871 NNS denotes reactions
T2807 7872-7876 VBD denotes were
T2806 7877-7887 VBN denotes terminated
T2808 7888-7890 IN denotes by
T2809 7891-7899 NN denotes addition
T2810 7900-7902 IN denotes of
T2811 7903-7907 NN denotes EDTA
T2812 7908-7909 -LRB- denotes (
T2814 7909-7911 CD denotes 20
T2813 7912-7914 NN denotes mM
T2815 7914-7915 -RRB- denotes )
T2816 7915-7917 , denotes ,
T2817 7917-7920 NN denotes SDS
T2818 7921-7922 -LRB- denotes (
T2820 7922-7925 CD denotes 0.1
T2819 7925-7926 NN denotes %
T2821 7926-7927 -RRB- denotes )
T2822 7928-7931 CC denotes and
T2823 7932-7933 CD denotes 2
T2824 7934-7936 NN denotes μg
T2826 7937-7947 NN denotes proteinase
T2825 7948-7949 NN denotes K
T2827 7949-7951 , denotes ,
T2828 7951-7954 CC denotes and
T2829 7955-7959 VBD denotes were
T2830 7960-7969 VBN denotes incubated
T2831 7970-7973 IN denotes for
T2832 7974-7984 JJ denotes additional
T2834 7985-7987 CD denotes 15
T2833 7988-7991 NN denotes min
T2835 7991-7992 . denotes .
T2836 7992-8085 sentence denotes The samples were separated by electrophoresis on nondenaturing polyacrylamide gel in 1× TBE.
T2837 7993-7996 DT denotes The
T2838 7997-8004 NNS denotes samples
T2840 8005-8009 VBD denotes were
T2839 8010-8019 VBN denotes separated
T2841 8020-8022 IN denotes by
T2842 8023-8038 NN denotes electrophoresis
T2843 8039-8041 IN denotes on
T2844 8042-8055 JJ denotes nondenaturing
T2846 8056-8070 NN denotes polyacrylamide
T2845 8071-8074 NN denotes gel
T2847 8075-8077 IN denotes in
T2848 8078-8079 CD denotes 1
T2850 8079-8080 SYM denotes ×
T2849 8081-8084 NN denotes TBE
T2851 8084-8085 . denotes .
T2967 8087-8095 NN denotes Nuclease
T2968 8096-8106 NN denotes protection
T2969 8107-8113 NNS denotes assays
T2970 8113-8365 sentence denotes The reaction mixtures (25 μl) contained 25 mM HEPES–NaOH (pH 7.5), 50 mM sodium acetate, 10 mM Mg(CH3COO)2, 1 mM DTT, 0.25 mg/ml BSA, 0.5 mM ATP-γ-S, 0.5 mM CaCl2, 10 fmol of 32P-labeled bubble substrate, and the indicated amount of Mcm4/6/7 proteins.
T2971 8114-8117 DT denotes The
T2973 8118-8126 NN denotes reaction
T2972 8127-8135 NNS denotes mixtures
T2975 8136-8137 -LRB- denotes (
T2977 8137-8139 CD denotes 25
T2976 8140-8142 NN denotes μl
T2978 8142-8143 -RRB- denotes )
T2974 8144-8153 VBD denotes contained
T2979 8154-8156 CD denotes 25
T2980 8157-8159 NN denotes mM
T2982 8160-8165 NN denotes HEPES
T2983 8165-8166 HYPH denotes –
T2981 8166-8170 NN denotes NaOH
T2984 8171-8172 -LRB- denotes (
T2985 8172-8174 NN denotes pH
T2986 8175-8178 CD denotes 7.5
T2987 8178-8179 -RRB- denotes )
T2988 8179-8181 , denotes ,
T2989 8181-8183 CD denotes 50
T2990 8184-8186 NN denotes mM
T2992 8187-8193 NN denotes sodium
T2991 8194-8201 NN denotes acetate
T2993 8201-8203 , denotes ,
T2994 8203-8205 CD denotes 10
T2995 8206-8208 NN denotes mM
T2997 8209-8211 NN denotes Mg
T2998 8211-8212 -LRB- denotes (
T2996 8212-8218 NN denotes CH3COO
T2999 8218-8219 -RRB- denotes )
T3000 8219-8220 CD denotes 2
T3001 8220-8222 , denotes ,
T3002 8222-8223 CD denotes 1
T3003 8224-8226 NN denotes mM
T3004 8227-8230 NN denotes DTT
T3005 8230-8232 , denotes ,
T3006 8232-8236 CD denotes 0.25
T3007 8237-8239 NN denotes mg
T3009 8239-8240 SYM denotes /
T3010 8240-8242 NN denotes ml
T3008 8243-8246 NN denotes BSA
T3011 8246-8248 , denotes ,
T3012 8248-8251 CD denotes 0.5
T3013 8252-8254 NN denotes mM
T3015 8255-8258 NN denotes ATP
T3016 8258-8259 HYPH denotes -
T3017 8259-8260 SYM denotes γ
T3018 8260-8261 HYPH denotes -
T3014 8261-8262 NN denotes S
T3019 8262-8264 , denotes ,
T3020 8264-8267 CD denotes 0.5
T3021 8268-8270 NN denotes mM
T3022 8271-8276 NN denotes CaCl2
T3023 8276-8278 , denotes ,
T3024 8278-8280 CD denotes 10
T3025 8281-8285 NN denotes fmol
T3026 8286-8288 IN denotes of
T3027 8289-8292 NN denotes 32P
T3029 8292-8293 HYPH denotes -
T3028 8293-8300 VBN denotes labeled
T3031 8301-8307 NN denotes bubble
T3030 8308-8317 NN denotes substrate
T3032 8317-8319 , denotes ,
T3033 8319-8322 CC denotes and
T3034 8323-8326 DT denotes the
T3036 8327-8336 VBN denotes indicated
T3035 8337-8343 NN denotes amount
T3037 8344-8346 IN denotes of
T3038 8347-8351 NN denotes Mcm4
T3040 8351-8352 HYPH denotes /
T3041 8352-8353 CD denotes 6
T3042 8353-8354 HYPH denotes /
T3043 8354-8355 CD denotes 7
T3039 8356-8364 NN denotes proteins
T3044 8364-8365 . denotes .
T3045 8365-8535 sentence denotes After incubation at 30°C for 30 min, the indicated amount of DNase I (TAKARA Biomedical, Japan) was added, and the mixtures were incubated at room temperature for 1 min.
T3046 8366-8371 IN denotes After
T3048 8372-8382 NN denotes incubation
T3049 8383-8385 IN denotes at
T3050 8386-8388 CD denotes 30
T3051 8388-8390 NN denotes °C
T3052 8391-8394 IN denotes for
T3053 8395-8397 CD denotes 30
T3054 8398-8401 NN denotes min
T3055 8401-8403 , denotes ,
T3056 8403-8406 DT denotes the
T3058 8407-8416 VBN denotes indicated
T3057 8417-8423 NN denotes amount
T3059 8424-8426 IN denotes of
T3060 8427-8432 NN denotes DNase
T3061 8433-8434 CD denotes I
T3062 8435-8436 -LRB- denotes (
T3064 8436-8442 NNP denotes TAKARA
T3063 8443-8453 NNP denotes Biomedical
T3065 8453-8455 , denotes ,
T3066 8455-8460 NNP denotes Japan
T3067 8460-8461 -RRB- denotes )
T3068 8462-8465 VBD denotes was
T3047 8466-8471 VBN denotes added
T3069 8471-8473 , denotes ,
T3070 8473-8476 CC denotes and
T3071 8477-8480 DT denotes the
T3072 8481-8489 NNS denotes mixtures
T3074 8490-8494 VBD denotes were
T3073 8495-8504 VBN denotes incubated
T3075 8505-8507 IN denotes at
T3076 8508-8512 NN denotes room
T3077 8513-8524 NN denotes temperature
T3078 8525-8528 IN denotes for
T3079 8529-8530 CD denotes 1
T3080 8531-8534 NN denotes min
T3081 8534-8535 . denotes .
T3082 8535-8671 sentence denotes DNase I was inactivated by the addition of 250 μl of stop solution (20 mM EDTA, 0.2M sodium chloride, 1% SDS and 12.5 μg/ml yeast RNA).
T3083 8536-8541 NN denotes DNase
T3085 8542-8543 CD denotes I
T3086 8544-8547 VBD denotes was
T3084 8548-8559 VBN denotes inactivated
T3087 8560-8562 IN denotes by
T3088 8563-8566 DT denotes the
T3089 8567-8575 NN denotes addition
T3090 8576-8578 IN denotes of
T3091 8579-8582 CD denotes 250
T3092 8583-8585 NN denotes μl
T3093 8586-8588 IN denotes of
T3094 8589-8593 NN denotes stop
T3095 8594-8602 NN denotes solution
T3096 8603-8604 -LRB- denotes (
T3098 8604-8606 CD denotes 20
T3099 8607-8609 NN denotes mM
T3097 8610-8614 NN denotes EDTA
T3100 8614-8616 , denotes ,
T3101 8616-8620 NN denotes 0.2M
T3103 8621-8627 NN denotes sodium
T3102 8628-8636 NN denotes chloride
T3104 8636-8638 , denotes ,
T3105 8638-8639 CD denotes 1
T3106 8639-8640 NN denotes %
T3107 8641-8644 NN denotes SDS
T3108 8645-8648 CC denotes and
T3109 8649-8653 CD denotes 12.5
T3110 8654-8656 NN denotes μg
T3112 8656-8657 SYM denotes /
T3113 8657-8659 NN denotes ml
T3114 8660-8665 NN denotes yeast
T3111 8666-8669 NN denotes RNA
T3115 8669-8670 -RRB- denotes )
T3116 8670-8671 . denotes .
T3117 8671-8799 sentence denotes For nuclease P1 footprinting assay, binding reactions were carried out under the same conditions except that CaCl2 was omitted.
T3118 8672-8675 IN denotes For
T3120 8676-8684 NN denotes nuclease
T3122 8685-8687 NN denotes P1
T3121 8688-8700 NN denotes footprinting
T3123 8701-8706 NN denotes assay
T3124 8706-8708 , denotes ,
T3125 8708-8715 NN denotes binding
T3126 8716-8725 NNS denotes reactions
T3127 8726-8730 VBD denotes were
T3119 8731-8738 VBN denotes carried
T3128 8739-8742 RP denotes out
T3129 8743-8748 IN denotes under
T3130 8749-8752 DT denotes the
T3132 8753-8757 JJ denotes same
T3131 8758-8768 NNS denotes conditions
T3133 8769-8775 IN denotes except
T3134 8776-8780 IN denotes that
T3136 8781-8786 NN denotes CaCl2
T3137 8787-8790 VBD denotes was
T3135 8791-8798 VBN denotes omitted
T3138 8798-8799 . denotes .
T3139 8799-8884 sentence denotes P1 nuclease (0.3 U, Roche) was added and incubation was continued at 37°C for 1 min.
T3140 8800-8802 NN denotes P1
T3141 8803-8811 NN denotes nuclease
T3143 8812-8813 -LRB- denotes (
T3145 8813-8816 CD denotes 0.3
T3146 8817-8818 NNP denotes U
T3147 8818-8820 , denotes ,
T3144 8820-8825 NNP denotes Roche
T3148 8825-8826 -RRB- denotes )
T3149 8827-8830 VBD denotes was
T3142 8831-8836 VBN denotes added
T3150 8837-8840 CC denotes and
T3151 8841-8851 NN denotes incubation
T3153 8852-8855 VBD denotes was
T3152 8856-8865 VBN denotes continued
T3154 8866-8868 IN denotes at
T3155 8869-8871 CD denotes 37
T3156 8871-8873 NN denotes °C
T3157 8874-8877 IN denotes for
T3158 8878-8879 CD denotes 1
T3159 8880-8883 NN denotes min
T3160 8883-8884 . denotes .
T3161 8884-9079 sentence denotes Cleavage was stopped by addition of 50 μl of 25 mM EDTA and 0.4 M NaOH; the mixture was incubated at room temperature for 10 min followed by the addition of 12 μl of 3 M sodium acetate (pH 4.8).
T3162 8885-8893 NN denotes Cleavage
T3164 8894-8897 VBD denotes was
T3163 8898-8905 VBN denotes stopped
T3166 8906-8908 IN denotes by
T3167 8909-8917 NN denotes addition
T3168 8918-8920 IN denotes of
T3169 8921-8923 CD denotes 50
T3170 8924-8926 NN denotes μl
T3171 8927-8929 IN denotes of
T3172 8930-8932 CD denotes 25
T3173 8933-8935 NN denotes mM
T3174 8936-8940 NN denotes EDTA
T3175 8941-8944 CC denotes and
T3176 8945-8948 CD denotes 0.4
T3177 8949-8950 NN denotes M
T3178 8951-8955 NN denotes NaOH
T3179 8955-8956 : denotes ;
T3180 8957-8960 DT denotes the
T3181 8961-8968 NN denotes mixture
T3182 8969-8972 VBD denotes was
T3165 8973-8982 VBN denotes incubated
T3183 8983-8985 IN denotes at
T3184 8986-8990 NN denotes room
T3185 8991-9002 NN denotes temperature
T3186 9003-9006 IN denotes for
T3187 9007-9009 CD denotes 10
T3188 9010-9013 NN denotes min
T3189 9014-9022 VBN denotes followed
T3190 9023-9025 IN denotes by
T3191 9026-9029 DT denotes the
T3192 9030-9038 NN denotes addition
T3193 9039-9041 IN denotes of
T3194 9042-9044 CD denotes 12
T3195 9045-9047 NN denotes μl
T3196 9048-9050 IN denotes of
T3197 9051-9052 CD denotes 3
T3198 9053-9054 NN denotes M
T3200 9055-9061 NN denotes sodium
T3199 9062-9069 NN denotes acetate
T3201 9070-9071 -LRB- denotes (
T3202 9071-9073 NN denotes pH
T3203 9074-9077 CD denotes 4.8
T3204 9077-9078 -RRB- denotes )
T3205 9078-9079 . denotes .
T3206 9079-9218 sentence denotes Proteins were removed by phenol–chloroform extraction, and DNAs were collected by ethanol precipitation followed by wash with 70% ethanol.
T3207 9080-9088 NNS denotes Proteins
T3209 9089-9093 VBD denotes were
T3208 9094-9101 VBN denotes removed
T3210 9102-9104 IN denotes by
T3211 9105-9111 NN denotes phenol
T3213 9111-9112 HYPH denotes –
T3212 9112-9122 NN denotes chloroform
T3214 9123-9133 NN denotes extraction
T3215 9133-9135 , denotes ,
T3216 9135-9138 CC denotes and
T3217 9139-9143 NNS denotes DNAs
T3219 9144-9148 VBD denotes were
T3218 9149-9158 VBN denotes collected
T3220 9159-9161 IN denotes by
T3221 9162-9169 NN denotes ethanol
T3222 9170-9183 NN denotes precipitation
T3223 9184-9192 VBN denotes followed
T3224 9193-9195 IN denotes by
T3225 9196-9200 NN denotes wash
T3226 9201-9205 IN denotes with
T3227 9206-9208 CD denotes 70
T3228 9208-9209 NN denotes %
T3229 9210-9217 NN denotes ethanol
T3230 9217-9218 . denotes .
T3231 9218-9368 sentence denotes The digested DNA was subjected to electrophoresis through a 10 or 12% polyacrylamide sequencing gel containing 7 M urea, followed by autoradiography.
T3232 9219-9222 DT denotes The
T3234 9223-9231 VBN denotes digested
T3233 9232-9235 NN denotes DNA
T3236 9236-9239 VBD denotes was
T3235 9240-9249 VBN denotes subjected
T3237 9250-9252 IN denotes to
T3238 9253-9268 NN denotes electrophoresis
T3239 9269-9276 IN denotes through
T3240 9277-9278 DT denotes a
T3242 9279-9281 CD denotes 10
T3244 9282-9284 CC denotes or
T3245 9285-9287 CD denotes 12
T3243 9287-9288 NN denotes %
T3246 9289-9303 NN denotes polyacrylamide
T3247 9304-9314 NN denotes sequencing
T3241 9315-9318 NN denotes gel
T3248 9319-9329 VBG denotes containing
T3249 9330-9331 CD denotes 7
T3250 9332-9333 NN denotes M
T3251 9334-9338 NN denotes urea
T3252 9338-9340 , denotes ,
T3253 9340-9348 VBN denotes followed
T3254 9349-9351 IN denotes by
T3255 9352-9367 NN denotes autoradiography
T3256 9367-9368 . denotes .
T3428 9379-9383 NN denotes Mcm4
T3430 9383-9384 HYPH denotes /
T3431 9384-9385 CD denotes 6
T3432 9385-9386 HYPH denotes /
T3433 9386-9387 CD denotes 7
T3429 9388-9395 NN denotes complex
T3435 9396-9405 RB denotes primarily
T3434 9406-9415 VBZ denotes interacts
T3436 9416-9420 IN denotes with
T3437 9421-9424 DT denotes the
T3439 9425-9431 JJ denotes single
T3441 9431-9432 HYPH denotes -
T3440 9432-9440 VBN denotes stranded
T3442 9441-9444 NN denotes DNA
T3438 9445-9452 NN denotes segment
T3443 9452-9600 sentence denotes Mcm4/6/7 binds to ‘bubble’ DNA substrates and unwinds the duplex segments when the central single-stranded segment contains thymine stretches (13).
T3444 9453-9457 NN denotes Mcm4
T3446 9457-9458 HYPH denotes /
T3447 9458-9459 CD denotes 6
T3448 9459-9460 HYPH denotes /
T3449 9460-9461 CD denotes 7
T3445 9462-9467 VBZ denotes binds
T3450 9468-9470 IN denotes to
T3451 9471-9472 `` denotes ‘
T3453 9472-9478 NN denotes bubble
T3454 9478-9479 '' denotes ’
T3455 9480-9483 NN denotes DNA
T3452 9484-9494 NNS denotes substrates
T3456 9495-9498 CC denotes and
T3457 9499-9506 VBZ denotes unwinds
T3458 9507-9510 DT denotes the
T3460 9511-9517 NN denotes duplex
T3459 9518-9526 NNS denotes segments
T3461 9527-9531 WRB denotes when
T3463 9532-9535 DT denotes the
T3465 9536-9543 JJ denotes central
T3466 9544-9550 JJ denotes single
T3468 9550-9551 HYPH denotes -
T3467 9551-9559 VBN denotes stranded
T3464 9560-9567 NN denotes segment
T3462 9568-9576 VBZ denotes contains
T3469 9577-9584 NN denotes thymine
T3470 9585-9594 NNS denotes stretches
T3471 9595-9596 -LRB- denotes (
T3472 9596-9598 CD denotes 13
T3473 9598-9599 -RRB- denotes )
T3474 9599-9600 . denotes .
T3475 9600-9859 sentence denotes In order to examine the mode of binding of Mcm4/6/7 on bubble DNAs, we conducted nuclease protection assays using labeled bubble substrate DNAs and Mcm4/6/7 in the presence of ATP-γ-S which permits binding to substrate DNA but does not mobilize the helicase.
T3476 9601-9603 IN denotes In
T3478 9604-9609 NN denotes order
T3479 9610-9612 TO denotes to
T3480 9613-9620 VB denotes examine
T3481 9621-9624 DT denotes the
T3482 9625-9629 NN denotes mode
T3483 9630-9632 IN denotes of
T3484 9633-9640 NN denotes binding
T3485 9641-9643 IN denotes of
T3486 9644-9648 NN denotes Mcm4
T3487 9648-9649 HYPH denotes /
T3488 9649-9650 CD denotes 6
T3489 9650-9651 HYPH denotes /
T3490 9651-9652 CD denotes 7
T3491 9653-9655 IN denotes on
T3492 9656-9662 NN denotes bubble
T3493 9663-9667 NNS denotes DNAs
T3494 9667-9669 , denotes ,
T3495 9669-9671 PRP denotes we
T3477 9672-9681 VBD denotes conducted
T3496 9682-9690 NN denotes nuclease
T3498 9691-9701 NN denotes protection
T3497 9702-9708 NNS denotes assays
T3499 9709-9714 VBG denotes using
T3500 9715-9722 VBN denotes labeled
T3502 9723-9729 NN denotes bubble
T3503 9730-9739 NN denotes substrate
T3501 9740-9744 NNS denotes DNAs
T3504 9745-9748 CC denotes and
T3505 9749-9753 NN denotes Mcm4
T3506 9753-9754 HYPH denotes /
T3507 9754-9755 CD denotes 6
T3508 9755-9756 HYPH denotes /
T3509 9756-9757 CD denotes 7
T3510 9758-9760 IN denotes in
T3511 9761-9764 DT denotes the
T3512 9765-9773 NN denotes presence
T3513 9774-9776 IN denotes of
T3514 9777-9780 NN denotes ATP
T3516 9780-9781 HYPH denotes -
T3517 9781-9782 SYM denotes γ
T3518 9782-9783 HYPH denotes -
T3515 9783-9784 NN denotes S
T3519 9785-9790 WDT denotes which
T3520 9791-9798 VBZ denotes permits
T3521 9799-9806 NN denotes binding
T3522 9807-9809 IN denotes to
T3523 9810-9819 NN denotes substrate
T3524 9820-9823 NN denotes DNA
T3525 9824-9827 CC denotes but
T3526 9828-9832 VBZ denotes does
T3528 9833-9836 RB denotes not
T3527 9837-9845 VB denotes mobilize
T3529 9846-9849 DT denotes the
T3530 9850-9858 NN denotes helicase
T3531 9858-9859 . denotes .
T3532 9859-10130 sentence denotes At an optimum concentration of DNase I, strong cleavages were detected on the 21 bp duplex regions at both ends of the substrates, whereas weaker cleavages were detected on the central single-stranded region, consistent with preference of DNase I to double-stranded DNA.
T3533 9860-9862 IN denotes At
T3535 9863-9865 DT denotes an
T3537 9866-9873 JJ denotes optimum
T3536 9874-9887 NN denotes concentration
T3538 9888-9890 IN denotes of
T3539 9891-9896 NN denotes DNase
T3540 9897-9898 CD denotes I
T3541 9898-9900 , denotes ,
T3542 9900-9906 JJ denotes strong
T3543 9907-9916 NNS denotes cleavages
T3544 9917-9921 VBD denotes were
T3534 9922-9930 VBN denotes detected
T3545 9931-9933 IN denotes on
T3546 9934-9937 DT denotes the
T3548 9938-9940 CD denotes 21
T3549 9941-9943 NN denotes bp
T3550 9944-9950 NN denotes duplex
T3547 9951-9958 NNS denotes regions
T3551 9959-9961 IN denotes at
T3552 9962-9966 DT denotes both
T3553 9967-9971 NNS denotes ends
T3554 9972-9974 IN denotes of
T3555 9975-9978 DT denotes the
T3556 9979-9989 NNS denotes substrates
T3557 9989-9991 , denotes ,
T3558 9991-9998 IN denotes whereas
T3560 9999-10005 JJR denotes weaker
T3561 10006-10015 NNS denotes cleavages
T3562 10016-10020 VBD denotes were
T3559 10021-10029 VBN denotes detected
T3563 10030-10032 IN denotes on
T3564 10033-10036 DT denotes the
T3566 10037-10044 JJ denotes central
T3567 10045-10051 JJ denotes single
T3569 10051-10052 HYPH denotes -
T3568 10052-10060 VBN denotes stranded
T3565 10061-10067 NN denotes region
T3570 10067-10069 , denotes ,
T3571 10069-10079 JJ denotes consistent
T3572 10080-10084 IN denotes with
T3573 10085-10095 NN denotes preference
T3574 10096-10098 IN denotes of
T3575 10099-10104 NN denotes DNase
T3576 10105-10106 CD denotes I
T3577 10107-10109 IN denotes to
T3578 10110-10116 JJ denotes double
T3580 10116-10117 HYPH denotes -
T3579 10117-10125 VBN denotes stranded
T3581 10126-10129 NN denotes DNA
T3582 10129-10130 . denotes .
T3583 10130-10388 sentence denotes With the Bub66/T-rich substrate, strong protection was detected on the entire single-stranded regions of both top and bottom strands with increasing concentration of Mcm4/6/7, consistent with high affinity of Mcm4/6/7 to T-rich bubble sequences (Figure 1A).
T3584 10131-10135 IN denotes With
T3586 10136-10139 DT denotes the
T3588 10140-10145 NN denotes Bub66
T3589 10145-10146 HYPH denotes /
T3590 10146-10147 NN denotes T
T3591 10147-10148 HYPH denotes -
T3592 10148-10152 JJ denotes rich
T3587 10153-10162 NN denotes substrate
T3593 10162-10164 , denotes ,
T3594 10164-10170 JJ denotes strong
T3595 10171-10181 NN denotes protection
T3596 10182-10185 VBD denotes was
T3585 10186-10194 VBN denotes detected
T3597 10195-10197 IN denotes on
T3598 10198-10201 DT denotes the
T3600 10202-10208 JJ denotes entire
T3601 10209-10215 JJ denotes single
T3603 10215-10216 HYPH denotes -
T3602 10216-10224 VBN denotes stranded
T3599 10225-10232 NNS denotes regions
T3604 10233-10235 IN denotes of
T3605 10236-10240 CC denotes both
T3606 10241-10244 JJ denotes top
T3608 10245-10248 CC denotes and
T3609 10249-10255 JJ denotes bottom
T3607 10256-10263 NNS denotes strands
T3610 10264-10268 IN denotes with
T3611 10269-10279 VBG denotes increasing
T3612 10280-10293 NN denotes concentration
T3613 10294-10296 IN denotes of
T3614 10297-10301 NN denotes Mcm4
T3615 10301-10302 HYPH denotes /
T3616 10302-10303 CD denotes 6
T3617 10303-10304 HYPH denotes /
T3618 10304-10305 CD denotes 7
T3619 10305-10307 , denotes ,
T3620 10307-10317 JJ denotes consistent
T3621 10318-10322 IN denotes with
T3622 10323-10327 JJ denotes high
T3623 10328-10336 NN denotes affinity
T3624 10337-10339 IN denotes of
T3625 10340-10344 NN denotes Mcm4
T3626 10344-10345 HYPH denotes /
T3627 10345-10346 CD denotes 6
T3628 10346-10347 HYPH denotes /
T3629 10347-10348 CD denotes 7
T3630 10349-10351 IN denotes to
T3631 10352-10353 NN denotes T
T3633 10353-10354 HYPH denotes -
T3634 10354-10358 JJ denotes rich
T3635 10359-10365 NN denotes bubble
T3632 10366-10375 NNS denotes sequences
T3636 10376-10377 -LRB- denotes (
T3638 10377-10383 NN denotes Figure
T3637 10384-10386 NN denotes 1A
T3639 10386-10387 -RRB- denotes )
T3640 10387-10388 . denotes .
T3641 10388-10550 sentence denotes Similar, but less significant protection was detected on the single-stranded DNA segment with P1 nucleases (Figure 1A), which is specific to single-stranded DNA.
T3642 10389-10396 JJ denotes Similar
T3644 10396-10398 , denotes ,
T3645 10398-10401 CC denotes but
T3646 10402-10406 RBR denotes less
T3647 10407-10418 JJ denotes significant
T3643 10419-10429 NN denotes protection
T3649 10430-10433 VBD denotes was
T3648 10434-10442 VBN denotes detected
T3650 10443-10445 IN denotes on
T3651 10446-10449 DT denotes the
T3653 10450-10456 JJ denotes single
T3655 10456-10457 HYPH denotes -
T3654 10457-10465 VBN denotes stranded
T3656 10466-10469 NN denotes DNA
T3652 10470-10477 NN denotes segment
T3657 10478-10482 IN denotes with
T3658 10483-10485 NN denotes P1
T3659 10486-10495 NNS denotes nucleases
T3660 10496-10497 -LRB- denotes (
T3662 10497-10503 NN denotes Figure
T3661 10504-10506 NN denotes 1A
T3663 10506-10507 -RRB- denotes )
T3664 10507-10509 , denotes ,
T3665 10509-10514 WDT denotes which
T3666 10515-10517 VBZ denotes is
T3667 10518-10526 JJ denotes specific
T3668 10527-10529 IN denotes to
T3669 10530-10536 JJ denotes single
T3671 10536-10537 HYPH denotes -
T3670 10537-10545 VBN denotes stranded
T3672 10546-10549 NN denotes DNA
T3673 10549-10550 . denotes .
T3674 10550-10681 sentence denotes The results indicate that the Mcm complex is loaded onto bubble DNA through binding to both strands of the single-stranded region.
T3675 10551-10554 DT denotes The
T3676 10555-10562 NNS denotes results
T3677 10563-10571 VBP denotes indicate
T3678 10572-10576 IN denotes that
T3680 10577-10580 DT denotes the
T3682 10581-10584 NN denotes Mcm
T3681 10585-10592 NN denotes complex
T3683 10593-10595 VBZ denotes is
T3679 10596-10602 VBN denotes loaded
T3684 10603-10607 IN denotes onto
T3685 10608-10614 NN denotes bubble
T3686 10615-10618 NN denotes DNA
T3687 10619-10626 IN denotes through
T3688 10627-10634 NN denotes binding
T3689 10635-10637 IN denotes to
T3690 10638-10642 DT denotes both
T3691 10643-10650 NNS denotes strands
T3692 10651-10653 IN denotes of
T3693 10654-10657 DT denotes the
T3695 10658-10664 JJ denotes single
T3697 10664-10665 HYPH denotes -
T3696 10665-10673 VBN denotes stranded
T3694 10674-10680 NN denotes region
T3698 10680-10681 . denotes .
T3699 10681-10955 sentence denotes Careful examination of Mcm4/6/7 footprints on a bubble indicated strong protection on the 5′-half of the top strand and moderate protection on the remaining 3′-half, as well as on several base-pair duplex segment adjacent to the branch point (Figure 1A and data not shown).
T3700 10682-10689 JJ denotes Careful
T3701 10690-10701 NN denotes examination
T3703 10702-10704 IN denotes of
T3704 10705-10709 NN denotes Mcm4
T3706 10709-10710 HYPH denotes /
T3707 10710-10711 CD denotes 6
T3708 10711-10712 HYPH denotes /
T3709 10712-10713 CD denotes 7
T3705 10714-10724 NNS denotes footprints
T3710 10725-10727 IN denotes on
T3711 10728-10729 DT denotes a
T3712 10730-10736 NN denotes bubble
T3702 10737-10746 VBD denotes indicated
T3713 10747-10753 JJ denotes strong
T3714 10754-10764 NN denotes protection
T3715 10765-10767 IN denotes on
T3716 10768-10771 DT denotes the
T3718 10772-10773 CD denotes 5
T3719 10773-10774 SYM denotes ′
T3720 10774-10775 HYPH denotes -
T3717 10775-10779 NN denotes half
T3721 10780-10782 IN denotes of
T3722 10783-10786 DT denotes the
T3724 10787-10790 JJ denotes top
T3723 10791-10797 NN denotes strand
T3725 10798-10801 CC denotes and
T3726 10802-10810 JJ denotes moderate
T3727 10811-10821 NN denotes protection
T3728 10822-10824 IN denotes on
T3729 10825-10828 DT denotes the
T3731 10829-10838 VBG denotes remaining
T3732 10839-10840 CD denotes 3
T3733 10840-10841 SYM denotes ′
T3734 10841-10842 HYPH denotes -
T3730 10842-10846 NN denotes half
T3735 10846-10848 , denotes ,
T3736 10848-10850 RB denotes as
T3738 10851-10855 RB denotes well
T3737 10856-10858 IN denotes as
T3739 10859-10861 IN denotes on
T3740 10862-10869 JJ denotes several
T3742 10870-10874 NN denotes base
T3744 10874-10875 HYPH denotes -
T3743 10875-10879 NN denotes pair
T3745 10880-10886 NN denotes duplex
T3741 10887-10894 NN denotes segment
T3746 10895-10903 JJ denotes adjacent
T3747 10904-10906 IN denotes to
T3748 10907-10910 DT denotes the
T3750 10911-10917 NN denotes branch
T3749 10918-10923 NN denotes point
T3751 10924-10925 -LRB- denotes (
T3753 10925-10931 NN denotes Figure
T3752 10932-10934 NN denotes 1A
T3754 10935-10938 CC denotes and
T3755 10939-10943 NNS denotes data
T3756 10944-10947 RB denotes not
T3757 10948-10953 VBN denotes shown
T3758 10953-10954 -RRB- denotes )
T3759 10954-10955 . denotes .
T3760 10955-11027 sentence denotes A similar pattern of protection was observed also on the bottom strand.
T3761 10956-10957 DT denotes A
T3763 10958-10965 JJ denotes similar
T3762 10966-10973 NN denotes pattern
T3765 10974-10976 IN denotes of
T3766 10977-10987 NN denotes protection
T3767 10988-10991 VBD denotes was
T3764 10992-11000 VBN denotes observed
T3768 11001-11005 RB denotes also
T3769 11006-11008 IN denotes on
T3770 11009-11012 DT denotes the
T3772 11013-11019 JJ denotes bottom
T3771 11020-11026 NN denotes strand
T3773 11026-11027 . denotes .
T3774 11027-11220 sentence denotes This suggests that Mcm4/6/7 may bind to a replication bubble substrate with a 2-fold symmetry as a double hexamer (Figure 1A, see the lower scheme), in a manner similar to SV40 T-antigen (21).
T3775 11028-11032 DT denotes This
T3776 11033-11041 VBZ denotes suggests
T3777 11042-11046 IN denotes that
T3779 11047-11051 NN denotes Mcm4
T3780 11051-11052 HYPH denotes /
T3781 11052-11053 CD denotes 6
T3782 11053-11054 HYPH denotes /
T3783 11054-11055 CD denotes 7
T3784 11056-11059 MD denotes may
T3778 11060-11064 VB denotes bind
T3785 11065-11067 IN denotes to
T3786 11068-11069 DT denotes a
T3788 11070-11081 NN denotes replication
T3789 11082-11088 NN denotes bubble
T3787 11089-11098 NN denotes substrate
T3790 11099-11103 IN denotes with
T3791 11104-11105 DT denotes a
T3793 11106-11112 CD denotes 2-fold
T3792 11113-11121 NN denotes symmetry
T3794 11122-11124 IN denotes as
T3795 11125-11126 DT denotes a
T3797 11127-11133 JJ denotes double
T3796 11134-11141 NN denotes hexamer
T3798 11142-11143 -LRB- denotes (
T3800 11143-11149 NN denotes Figure
T3799 11150-11152 NN denotes 1A
T3801 11152-11154 , denotes ,
T3802 11154-11157 VBP denotes see
T3803 11158-11161 DT denotes the
T3805 11162-11167 JJ denotes lower
T3804 11168-11174 NN denotes scheme
T3806 11174-11175 -RRB- denotes )
T3807 11175-11177 , denotes ,
T3808 11177-11179 IN denotes in
T3809 11180-11181 DT denotes a
T3810 11182-11188 NN denotes manner
T3811 11189-11196 JJ denotes similar
T3812 11197-11199 IN denotes to
T3813 11200-11204 NN denotes SV40
T3815 11205-11206 NN denotes T
T3816 11206-11207 HYPH denotes -
T3814 11207-11214 NN denotes antigen
T3817 11215-11216 -LRB- denotes (
T3818 11216-11218 CD denotes 21
T3819 11218-11219 -RRB- denotes )
T3820 11219-11220 . denotes .
T3821 11220-11311 sentence denotes Nuclease protection assays were conducted also on a T-tailed Y-fork structure (Figure 1B).
T3822 11221-11229 NN denotes Nuclease
T3823 11230-11240 NN denotes protection
T3824 11241-11247 NNS denotes assays
T3826 11248-11252 VBD denotes were
T3825 11253-11262 VBN denotes conducted
T3827 11263-11267 RB denotes also
T3828 11268-11270 IN denotes on
T3829 11271-11272 DT denotes a
T3831 11273-11274 NN denotes T
T3833 11274-11275 HYPH denotes -
T3832 11275-11281 VBN denotes tailed
T3834 11282-11283 NN denotes Y
T3836 11283-11284 HYPH denotes -
T3835 11284-11288 NN denotes fork
T3830 11289-11298 NN denotes structure
T3837 11299-11300 -LRB- denotes (
T3839 11300-11306 NN denotes Figure
T3838 11307-11309 NN denotes 1B
T3840 11309-11310 -RRB- denotes )
T3841 11310-11311 . denotes .
T3842 11311-11483 sentence denotes Not only the single-stranded tail region but also the 7 bp duplex DNA adjacent to the duplex-to-single-strand junction were protected by Mcm binding from DNaseI digestion.
T3843 11312-11315 RB denotes Not
T3845 11316-11320 RB denotes only
T3846 11321-11324 DT denotes the
T3847 11325-11331 JJ denotes single
T3849 11331-11332 HYPH denotes -
T3848 11332-11340 VBN denotes stranded
T3850 11341-11345 NN denotes tail
T3844 11346-11352 NN denotes region
T3852 11353-11356 CC denotes but
T3853 11357-11361 RB denotes also
T3854 11362-11365 DT denotes the
T3856 11366-11367 CD denotes 7
T3857 11368-11370 NN denotes bp
T3858 11371-11377 NN denotes duplex
T3855 11378-11381 NN denotes DNA
T3859 11382-11390 JJ denotes adjacent
T3860 11391-11393 IN denotes to
T3861 11394-11397 DT denotes the
T3863 11398-11404 NN denotes duplex
T3864 11404-11405 HYPH denotes -
T3865 11405-11407 IN denotes to
T3866 11407-11408 HYPH denotes -
T3867 11408-11414 JJ denotes single
T3869 11414-11415 HYPH denotes -
T3868 11415-11421 NN denotes strand
T3862 11422-11430 NN denotes junction
T3870 11431-11435 VBD denotes were
T3851 11436-11445 VBN denotes protected
T3871 11446-11448 IN denotes by
T3872 11449-11452 NN denotes Mcm
T3873 11453-11460 NN denotes binding
T3874 11461-11465 IN denotes from
T3875 11466-11472 NN denotes DNaseI
T3876 11473-11482 NN denotes digestion
T3877 11482-11483 . denotes .
T3878 11483-11682 sentence denotes These results indicate that Mcm4/6/7 protein primarily interacts with single-stranded DNA region and that the interaction also spans the duplex segments near the branchpoint of the replication fork.
T3879 11484-11489 DT denotes These
T3880 11490-11497 NNS denotes results
T3881 11498-11506 VBP denotes indicate
T3882 11507-11511 IN denotes that
T3884 11512-11516 NN denotes Mcm4
T3886 11516-11517 HYPH denotes /
T3887 11517-11518 CD denotes 6
T3888 11518-11519 HYPH denotes /
T3889 11519-11520 CD denotes 7
T3885 11521-11528 NN denotes protein
T3890 11529-11538 RB denotes primarily
T3883 11539-11548 VBZ denotes interacts
T3891 11549-11553 IN denotes with
T3892 11554-11560 JJ denotes single
T3894 11560-11561 HYPH denotes -
T3893 11561-11569 VBN denotes stranded
T3896 11570-11573 NN denotes DNA
T3895 11574-11580 NN denotes region
T3897 11581-11584 CC denotes and
T3898 11585-11589 IN denotes that
T3900 11590-11593 DT denotes the
T3901 11594-11605 NN denotes interaction
T3902 11606-11610 RB denotes also
T3899 11611-11616 VBZ denotes spans
T3903 11617-11620 DT denotes the
T3905 11621-11627 NN denotes duplex
T3904 11628-11636 NNS denotes segments
T3906 11637-11641 IN denotes near
T3907 11642-11645 DT denotes the
T3908 11646-11657 NN denotes branchpoint
T3909 11658-11660 IN denotes of
T3910 11661-11664 DT denotes the
T3912 11665-11676 NN denotes replication
T3911 11677-11681 NN denotes fork
T3913 11681-11682 . denotes .
T4077 11684-11693 NN denotes Formation
T4079 11694-11696 IN denotes of
T4080 11697-11703 JJ denotes double
T4081 11704-11713 JJ denotes hexameric
T4083 11714-11718 NN denotes Mcm4
T4084 11718-11719 HYPH denotes /
T4085 11719-11720 CD denotes 6
T4086 11720-11721 HYPH denotes /
T4087 11721-11722 CD denotes 7
T4082 11723-11732 NNS denotes complexes
T4078 11733-11744 VBZ denotes facilitates
T4088 11745-11753 NN denotes helicase
T4089 11754-11761 NNS denotes actions
T4090 11762-11764 IN denotes on
T4091 11765-11771 NN denotes bubble
T4092 11772-11782 NNS denotes substrates
T4093 11782-11972 sentence denotes The above results suggest that Mcm4/6/7 generates a double hexameric complex on a bubble, each hexamer of which makes symmetric contact with the 5′-proximal central single-stranded segment.
T4094 11783-11786 DT denotes The
T4096 11787-11792 JJ denotes above
T4095 11793-11800 NNS denotes results
T4097 11801-11808 VBP denotes suggest
T4098 11809-11813 IN denotes that
T4100 11814-11818 NN denotes Mcm4
T4101 11818-11819 HYPH denotes /
T4102 11819-11820 CD denotes 6
T4103 11820-11821 HYPH denotes /
T4104 11821-11822 CD denotes 7
T4099 11823-11832 VBZ denotes generates
T4105 11833-11834 DT denotes a
T4107 11835-11841 JJ denotes double
T4108 11842-11851 JJ denotes hexameric
T4106 11852-11859 NN denotes complex
T4109 11860-11862 IN denotes on
T4110 11863-11864 DT denotes a
T4111 11865-11871 NN denotes bubble
T4112 11871-11873 , denotes ,
T4113 11873-11877 DT denotes each
T4114 11878-11885 NN denotes hexamer
T4116 11886-11888 IN denotes of
T4117 11889-11894 WDT denotes which
T4115 11895-11900 VBZ denotes makes
T4118 11901-11910 JJ denotes symmetric
T4119 11911-11918 NN denotes contact
T4120 11919-11923 IN denotes with
T4121 11924-11927 DT denotes the
T4123 11928-11929 CD denotes 5
T4125 11929-11930 SYM denotes ′
T4126 11930-11931 HYPH denotes -
T4124 11931-11939 JJ denotes proximal
T4127 11940-11947 JJ denotes central
T4128 11948-11954 JJ denotes single
T4130 11954-11955 HYPH denotes -
T4129 11955-11963 VBN denotes stranded
T4122 11964-11971 NN denotes segment
T4131 11971-11972 . denotes .
T4132 11972-12095 sentence denotes This predicts that the single-stranded segment needs to be of a sufficient length in order to accommodate the Mcm complex.
T4133 11973-11977 DT denotes This
T4134 11978-11986 VBZ denotes predicts
T4135 11987-11991 IN denotes that
T4137 11992-11995 DT denotes the
T4139 11996-12002 JJ denotes single
T4141 12002-12003 HYPH denotes -
T4140 12003-12011 VBN denotes stranded
T4138 12012-12019 NN denotes segment
T4136 12020-12025 VBZ denotes needs
T4142 12026-12028 TO denotes to
T4143 12029-12031 VB denotes be
T4144 12032-12034 IN denotes of
T4145 12035-12036 DT denotes a
T4147 12037-12047 JJ denotes sufficient
T4146 12048-12054 NN denotes length
T4148 12055-12057 IN denotes in
T4149 12058-12063 NN denotes order
T4150 12064-12066 TO denotes to
T4151 12067-12078 VB denotes accommodate
T4152 12079-12082 DT denotes the
T4154 12083-12086 NN denotes Mcm
T4153 12087-12094 NN denotes complex
T4155 12094-12095 . denotes .
T4156 12095-12319 sentence denotes In fact, we previously showed that the Mcm4/6/7 mainly forms a single hexameric complex on bubble-20 (a bubble substrate with 20 nt long central single-stranded segment) and a double hexameric complex on bubble-60 DNA (13).
T4157 12096-12098 IN denotes In
T4159 12099-12103 NN denotes fact
T4160 12103-12105 , denotes ,
T4161 12105-12107 PRP denotes we
T4162 12108-12118 RB denotes previously
T4158 12119-12125 VBD denotes showed
T4163 12126-12130 IN denotes that
T4165 12131-12134 DT denotes the
T4166 12135-12139 NN denotes Mcm4
T4167 12139-12140 HYPH denotes /
T4168 12140-12141 CD denotes 6
T4169 12141-12142 HYPH denotes /
T4170 12142-12143 CD denotes 7
T4171 12144-12150 RB denotes mainly
T4164 12151-12156 VBZ denotes forms
T4172 12157-12158 DT denotes a
T4174 12159-12165 JJ denotes single
T4175 12166-12175 JJ denotes hexameric
T4173 12176-12183 NN denotes complex
T4176 12184-12186 IN denotes on
T4177 12187-12193 NN denotes bubble
T4178 12193-12194 HYPH denotes -
T4179 12194-12196 CD denotes 20
T4180 12197-12198 -LRB- denotes (
T4181 12198-12199 DT denotes a
T4183 12200-12206 NN denotes bubble
T4182 12207-12216 NN denotes substrate
T4184 12217-12221 IN denotes with
T4185 12222-12224 CD denotes 20
T4186 12225-12227 NN denotes nt
T4187 12228-12232 JJ denotes long
T4189 12233-12240 JJ denotes central
T4190 12241-12247 JJ denotes single
T4192 12247-12248 HYPH denotes -
T4191 12248-12256 VBN denotes stranded
T4188 12257-12264 NN denotes segment
T4193 12264-12265 -RRB- denotes )
T4194 12266-12269 CC denotes and
T4195 12270-12271 DT denotes a
T4197 12272-12278 JJ denotes double
T4198 12279-12288 JJ denotes hexameric
T4196 12289-12296 NN denotes complex
T4199 12297-12299 IN denotes on
T4200 12300-12306 NN denotes bubble
T4202 12306-12307 HYPH denotes -
T4203 12307-12309 CD denotes 60
T4201 12310-12313 NN denotes DNA
T4204 12314-12315 -LRB- denotes (
T4205 12315-12317 CD denotes 13
T4206 12317-12318 -RRB- denotes )
T4207 12318-12319 . denotes .
T4208 12319-12651 sentence denotes In order to determine the minimum requirement for double-hexamer formation, a set of synthetic bubble-like substrates (Bub-T10, Bub-T20, Bub-T30, Bub-T40 and Bub-T50) that differed in the length of the central unpaired segment were constructed, and the binding and helicase activities of Mcm4/6/7 on these substrates were examined.
T4209 12320-12322 IN denotes In
T4211 12323-12328 NN denotes order
T4212 12329-12331 TO denotes to
T4213 12332-12341 VB denotes determine
T4214 12342-12345 DT denotes the
T4216 12346-12353 JJ denotes minimum
T4215 12354-12365 NN denotes requirement
T4217 12366-12369 IN denotes for
T4218 12370-12376 JJ denotes double
T4220 12376-12377 HYPH denotes -
T4219 12377-12384 NN denotes hexamer
T4221 12385-12394 NN denotes formation
T4222 12394-12396 , denotes ,
T4223 12396-12397 DT denotes a
T4224 12398-12401 NN denotes set
T4225 12402-12404 IN denotes of
T4226 12405-12414 JJ denotes synthetic
T4228 12415-12421 NN denotes bubble
T4230 12421-12422 HYPH denotes -
T4229 12422-12426 JJ denotes like
T4227 12427-12437 NNS denotes substrates
T4231 12438-12439 -LRB- denotes (
T4232 12439-12442 NN denotes Bub
T4234 12442-12443 HYPH denotes -
T4233 12443-12446 NN denotes T10
T4235 12446-12448 , denotes ,
T4236 12448-12451 NN denotes Bub
T4238 12451-12452 HYPH denotes -
T4237 12452-12455 NN denotes T20
T4239 12455-12457 , denotes ,
T4240 12457-12460 NN denotes Bub
T4242 12460-12461 HYPH denotes -
T4241 12461-12464 NN denotes T30
T4243 12464-12466 , denotes ,
T4244 12466-12469 NN denotes Bub
T4246 12469-12470 HYPH denotes -
T4245 12470-12473 NN denotes T40
T4247 12474-12477 CC denotes and
T4248 12478-12481 NN denotes Bub
T4250 12481-12482 HYPH denotes -
T4249 12482-12485 NN denotes T50
T4251 12485-12486 -RRB- denotes )
T4252 12487-12491 WDT denotes that
T4253 12492-12500 VBD denotes differed
T4254 12501-12503 IN denotes in
T4255 12504-12507 DT denotes the
T4256 12508-12514 NN denotes length
T4257 12515-12517 IN denotes of
T4258 12518-12521 DT denotes the
T4260 12522-12529 JJ denotes central
T4261 12530-12538 JJ denotes unpaired
T4259 12539-12546 NN denotes segment
T4262 12547-12551 VBD denotes were
T4210 12552-12563 VBN denotes constructed
T4263 12563-12565 , denotes ,
T4264 12565-12568 CC denotes and
T4265 12569-12572 DT denotes the
T4267 12573-12580 NN denotes binding
T4268 12581-12584 CC denotes and
T4269 12585-12593 NN denotes helicase
T4266 12594-12604 NNS denotes activities
T4271 12605-12607 IN denotes of
T4272 12608-12612 NN denotes Mcm4
T4273 12612-12613 HYPH denotes /
T4274 12613-12614 CD denotes 6
T4275 12614-12615 HYPH denotes /
T4276 12615-12616 CD denotes 7
T4277 12617-12619 IN denotes on
T4278 12620-12625 DT denotes these
T4279 12626-12636 NNS denotes substrates
T4280 12637-12641 VBD denotes were
T4270 12642-12650 VBN denotes examined
T4281 12650-12651 . denotes .
T4282 12651-12783 sentence denotes Mcm4/6/7 was incubated with a radiolabeled synthetic bubble substrate in the presence of 0.5 mM ATP-γ-S to allow complex formation.
T4283 12652-12656 NN denotes Mcm4
T4285 12656-12657 HYPH denotes /
T4286 12657-12658 CD denotes 6
T4287 12658-12659 HYPH denotes /
T4288 12659-12660 CD denotes 7
T4289 12661-12664 VBD denotes was
T4284 12665-12674 VBN denotes incubated
T4290 12675-12679 IN denotes with
T4291 12680-12681 DT denotes a
T4293 12682-12694 VBN denotes radiolabeled
T4294 12695-12704 JJ denotes synthetic
T4295 12705-12711 NN denotes bubble
T4292 12712-12721 NN denotes substrate
T4296 12722-12724 IN denotes in
T4297 12725-12728 DT denotes the
T4298 12729-12737 NN denotes presence
T4299 12738-12740 IN denotes of
T4300 12741-12744 CD denotes 0.5
T4301 12745-12747 NN denotes mM
T4303 12748-12751 NN denotes ATP
T4304 12751-12752 HYPH denotes -
T4305 12752-12753 SYM denotes γ
T4306 12753-12754 HYPH denotes -
T4302 12754-12755 NN denotes S
T4307 12756-12758 TO denotes to
T4308 12759-12764 VB denotes allow
T4309 12765-12772 NN denotes complex
T4310 12773-12782 NN denotes formation
T4311 12782-12783 . denotes .
T4312 12783-12995 sentence denotes A half of these reaction mixtures were analyzed for DNA binding in gel shift assays (Figure 2A), and the remainder was further incubated in the presence of 10 mM ATP to measure DNA helicase activity (Figure 2B).
T4313 12784-12785 DT denotes A
T4314 12786-12790 NN denotes half
T4316 12791-12793 IN denotes of
T4317 12794-12799 DT denotes these
T4319 12800-12808 NN denotes reaction
T4318 12809-12817 NNS denotes mixtures
T4320 12818-12822 VBD denotes were
T4315 12823-12831 VBN denotes analyzed
T4321 12832-12835 IN denotes for
T4322 12836-12839 NN denotes DNA
T4323 12840-12847 NN denotes binding
T4324 12848-12850 IN denotes in
T4325 12851-12854 NN denotes gel
T4326 12855-12860 NN denotes shift
T4327 12861-12867 NNS denotes assays
T4328 12868-12869 -LRB- denotes (
T4330 12869-12875 NN denotes Figure
T4329 12876-12878 NN denotes 2A
T4331 12878-12879 -RRB- denotes )
T4332 12879-12881 , denotes ,
T4333 12881-12884 CC denotes and
T4334 12885-12888 DT denotes the
T4335 12889-12898 NN denotes remainder
T4337 12899-12902 VBD denotes was
T4338 12903-12910 RB denotes further
T4336 12911-12920 VBN denotes incubated
T4339 12921-12923 IN denotes in
T4340 12924-12927 DT denotes the
T4341 12928-12936 NN denotes presence
T4342 12937-12939 IN denotes of
T4343 12940-12942 CD denotes 10
T4344 12943-12945 NN denotes mM
T4345 12946-12949 NN denotes ATP
T4346 12950-12952 TO denotes to
T4347 12953-12960 VB denotes measure
T4348 12961-12964 NN denotes DNA
T4349 12965-12973 NN denotes helicase
T4350 12974-12982 NN denotes activity
T4351 12983-12984 -LRB- denotes (
T4353 12984-12990 NN denotes Figure
T4352 12991-12993 NN denotes 2B
T4354 12993-12994 -RRB- denotes )
T4355 12994-12995 . denotes .
T4356 12995-13168 sentence denotes Bub-10 generated only a low level of complexes with Mcm4/6/7, and Bub-20 generated primarily a single mobility-shifted form, which may contain a single hexamer of Mcm4/6/7.
T4357 12996-12999 NN denotes Bub
T4359 12999-13000 HYPH denotes -
T4360 13000-13002 CD denotes 10
T4358 13003-13012 VBD denotes generated
T4361 13013-13017 RB denotes only
T4363 13018-13019 DT denotes a
T4364 13020-13023 JJ denotes low
T4362 13024-13029 NN denotes level
T4365 13030-13032 IN denotes of
T4366 13033-13042 NNS denotes complexes
T4367 13043-13047 IN denotes with
T4368 13048-13052 NN denotes Mcm4
T4369 13052-13053 HYPH denotes /
T4370 13053-13054 CD denotes 6
T4371 13054-13055 HYPH denotes /
T4372 13055-13056 CD denotes 7
T4373 13056-13058 , denotes ,
T4374 13058-13061 CC denotes and
T4375 13062-13065 NN denotes Bub
T4377 13065-13066 HYPH denotes -
T4378 13066-13068 CD denotes 20
T4376 13069-13078 VBD denotes generated
T4379 13079-13088 RB denotes primarily
T4380 13089-13090 DT denotes a
T4382 13091-13097 JJ denotes single
T4383 13098-13106 NN denotes mobility
T4385 13106-13107 HYPH denotes -
T4384 13107-13114 VBN denotes shifted
T4381 13115-13119 NN denotes form
T4386 13119-13121 , denotes ,
T4387 13121-13126 WDT denotes which
T4389 13127-13130 MD denotes may
T4388 13131-13138 VB denotes contain
T4390 13139-13140 DT denotes a
T4392 13141-13147 JJ denotes single
T4391 13148-13155 NN denotes hexamer
T4393 13156-13158 IN denotes of
T4394 13159-13163 NN denotes Mcm4
T4395 13163-13164 HYPH denotes /
T4396 13164-13165 CD denotes 6
T4397 13165-13166 HYPH denotes /
T4398 13166-13167 CD denotes 7
T4399 13167-13168 . denotes .
T4400 13168-13324 sentence denotes On Bub-30, significant amount of complexes containing a double hexamer were generated in addition to those containing a single hexamer (Figure 2A) (13,21).
T4401 13169-13171 IN denotes On
T4403 13172-13175 NN denotes Bub
T4404 13175-13176 HYPH denotes -
T4405 13176-13178 CD denotes 30
T4406 13178-13180 , denotes ,
T4407 13180-13191 JJ denotes significant
T4408 13192-13198 NN denotes amount
T4409 13199-13201 IN denotes of
T4410 13202-13211 NNS denotes complexes
T4411 13212-13222 VBG denotes containing
T4412 13223-13224 DT denotes a
T4414 13225-13231 JJ denotes double
T4413 13232-13239 NN denotes hexamer
T4415 13240-13244 VBD denotes were
T4402 13245-13254 VBN denotes generated
T4416 13255-13257 IN denotes in
T4417 13258-13266 NN denotes addition
T4418 13267-13269 IN denotes to
T4419 13270-13275 DT denotes those
T4420 13276-13286 VBG denotes containing
T4421 13287-13288 DT denotes a
T4423 13289-13295 JJ denotes single
T4422 13296-13303 NN denotes hexamer
T4424 13304-13305 -LRB- denotes (
T4426 13305-13311 NN denotes Figure
T4425 13312-13314 NN denotes 2A
T4427 13314-13315 -RRB- denotes )
T4428 13316-13317 -LRB- denotes (
T4430 13317-13319 CD denotes 13
T4431 13319-13320 , denotes ,
T4429 13320-13322 CD denotes 21
T4432 13322-13323 -RRB- denotes )
T4433 13323-13324 . denotes .
T4434 13324-13464 sentence denotes The amounts of double hexamer complexes increased on Bub-T40 and Bub-T50 with concomitant decrease of single hexamer complexes (Figure 2A).
T4435 13325-13328 DT denotes The
T4436 13329-13336 NNS denotes amounts
T4438 13337-13339 IN denotes of
T4439 13340-13346 JJ denotes double
T4440 13347-13354 NN denotes hexamer
T4441 13355-13364 NNS denotes complexes
T4437 13365-13374 VBD denotes increased
T4442 13375-13377 IN denotes on
T4443 13378-13381 NN denotes Bub
T4445 13381-13382 HYPH denotes -
T4444 13382-13385 NN denotes T40
T4446 13386-13389 CC denotes and
T4447 13390-13393 NN denotes Bub
T4449 13393-13394 HYPH denotes -
T4448 13394-13397 NN denotes T50
T4450 13398-13402 IN denotes with
T4451 13403-13414 JJ denotes concomitant
T4452 13415-13423 NN denotes decrease
T4453 13424-13426 IN denotes of
T4454 13427-13433 JJ denotes single
T4455 13434-13441 NN denotes hexamer
T4456 13442-13451 NNS denotes complexes
T4457 13452-13453 -LRB- denotes (
T4459 13453-13459 NN denotes Figure
T4458 13460-13462 NN denotes 2A
T4460 13462-13463 -RRB- denotes )
T4461 13463-13464 . denotes .
T4462 13464-13633 sentence denotes The results indicate that a single-stranded segment of at least 40 nt long is required for efficient loading of the double-hexameric Mcm helicase on a bubble substrate.
T4463 13465-13468 DT denotes The
T4464 13469-13476 NNS denotes results
T4465 13477-13485 VBP denotes indicate
T4466 13486-13490 IN denotes that
T4468 13491-13492 DT denotes a
T4470 13493-13499 JJ denotes single
T4472 13499-13500 HYPH denotes -
T4471 13500-13508 VBN denotes stranded
T4469 13509-13516 NN denotes segment
T4473 13517-13519 IN denotes of
T4474 13520-13522 RB denotes at
T4475 13523-13528 RBS denotes least
T4476 13529-13531 CD denotes 40
T4477 13532-13534 NN denotes nt
T4478 13535-13539 JJ denotes long
T4479 13540-13542 VBZ denotes is
T4467 13543-13551 VBN denotes required
T4480 13552-13555 IN denotes for
T4481 13556-13565 JJ denotes efficient
T4482 13566-13573 NN denotes loading
T4483 13574-13576 IN denotes of
T4484 13577-13580 DT denotes the
T4486 13581-13587 JJ denotes double
T4488 13587-13588 HYPH denotes -
T4487 13588-13597 JJ denotes hexameric
T4489 13598-13601 NN denotes Mcm
T4485 13602-13610 NN denotes helicase
T4490 13611-13613 IN denotes on
T4491 13614-13615 DT denotes a
T4493 13616-13622 NN denotes bubble
T4492 13623-13632 NN denotes substrate
T4494 13632-13633 . denotes .
T4495 13633-13743 sentence denotes In DNA helicase assays, Mcm4/6/7 complex did not show helicase activity on either Bub-10 or Bub-20 substrate.
T4496 13634-13636 IN denotes In
T4498 13637-13640 NN denotes DNA
T4499 13641-13649 NN denotes helicase
T4500 13650-13656 NNS denotes assays
T4501 13656-13658 , denotes ,
T4502 13658-13662 NN denotes Mcm4
T4504 13662-13663 HYPH denotes /
T4505 13663-13664 CD denotes 6
T4506 13664-13665 HYPH denotes /
T4507 13665-13666 CD denotes 7
T4503 13667-13674 NN denotes complex
T4508 13675-13678 VBD denotes did
T4509 13679-13682 RB denotes not
T4497 13683-13687 VB denotes show
T4510 13688-13696 NN denotes helicase
T4511 13697-13705 NN denotes activity
T4512 13706-13708 IN denotes on
T4513 13709-13715 CC denotes either
T4514 13716-13719 NN denotes Bub
T4516 13719-13720 HYPH denotes -
T4517 13720-13722 CD denotes 10
T4518 13723-13725 CC denotes or
T4519 13726-13729 NN denotes Bub
T4520 13729-13730 HYPH denotes -
T4521 13730-13732 CD denotes 20
T4515 13733-13742 NN denotes substrate
T4522 13742-13743 . denotes .
T4523 13743-13860 sentence denotes The efficiency of displacement slightly improved as the length of the single-stranded segment increased up to 30 nt.
T4524 13744-13747 DT denotes The
T4525 13748-13758 NN denotes efficiency
T4527 13759-13761 IN denotes of
T4528 13762-13774 NN denotes displacement
T4529 13775-13783 RB denotes slightly
T4526 13784-13792 VBD denotes improved
T4530 13793-13795 IN denotes as
T4532 13796-13799 DT denotes the
T4533 13800-13806 NN denotes length
T4534 13807-13809 IN denotes of
T4535 13810-13813 DT denotes the
T4537 13814-13820 JJ denotes single
T4539 13820-13821 HYPH denotes -
T4538 13821-13829 VBN denotes stranded
T4536 13830-13837 NN denotes segment
T4531 13838-13847 VBD denotes increased
T4540 13848-13850 IN denotes up
T4542 13851-13853 IN denotes to
T4541 13854-13856 CD denotes 30
T4543 13857-13859 NN denotes nt
T4544 13859-13860 . denotes .
T4545 13860-14084 sentence denotes On Bub-T40 and Bub-T50, on the other hand, significant displacement was observed, indicating that a single-stranded segment of at least 40 nt long is required for efficient helicase actions on bubble substrates (Figure 2B).
T4546 13861-13863 IN denotes On
T4548 13864-13867 NN denotes Bub
T4550 13867-13868 HYPH denotes -
T4549 13868-13871 NN denotes T40
T4551 13872-13875 CC denotes and
T4552 13876-13879 NN denotes Bub
T4554 13879-13880 HYPH denotes -
T4553 13880-13883 NN denotes T50
T4555 13883-13885 , denotes ,
T4556 13885-13887 IN denotes on
T4557 13888-13891 DT denotes the
T4559 13892-13897 JJ denotes other
T4558 13898-13902 NN denotes hand
T4560 13902-13904 , denotes ,
T4561 13904-13915 JJ denotes significant
T4562 13916-13928 NN denotes displacement
T4563 13929-13932 VBD denotes was
T4547 13933-13941 VBN denotes observed
T4564 13941-13943 , denotes ,
T4565 13943-13953 VBG denotes indicating
T4566 13954-13958 IN denotes that
T4568 13959-13960 DT denotes a
T4570 13961-13967 JJ denotes single
T4572 13967-13968 HYPH denotes -
T4571 13968-13976 VBN denotes stranded
T4569 13977-13984 NN denotes segment
T4573 13985-13987 IN denotes of
T4574 13988-13990 RB denotes at
T4575 13991-13996 RBS denotes least
T4576 13997-13999 CD denotes 40
T4577 14000-14002 NN denotes nt
T4578 14003-14007 JJ denotes long
T4579 14008-14010 VBZ denotes is
T4567 14011-14019 VBN denotes required
T4580 14020-14023 IN denotes for
T4581 14024-14033 JJ denotes efficient
T4583 14034-14042 NN denotes helicase
T4582 14043-14050 NNS denotes actions
T4584 14051-14053 IN denotes on
T4585 14054-14060 NN denotes bubble
T4586 14061-14071 NNS denotes substrates
T4587 14072-14073 -LRB- denotes (
T4589 14073-14079 NN denotes Figure
T4588 14080-14082 NN denotes 2B
T4590 14082-14083 -RRB- denotes )
T4591 14083-14084 . denotes .
T4592 14084-14283 sentence denotes Taken together, the above results are consistent with the notion that the Mcm4/6/7 complex, loaded onto DNA as a double hexameric complex, efficiently unwinds the duplex region of the substrate DNA.
T4593 14085-14090 VBN denotes Taken
T4595 14091-14099 RB denotes together
T4596 14099-14101 , denotes ,
T4597 14101-14104 DT denotes the
T4599 14105-14110 JJ denotes above
T4598 14111-14118 NNS denotes results
T4594 14119-14122 VBP denotes are
T4600 14123-14133 JJ denotes consistent
T4601 14134-14138 IN denotes with
T4602 14139-14142 DT denotes the
T4603 14143-14149 NN denotes notion
T4604 14150-14154 IN denotes that
T4606 14155-14158 DT denotes the
T4608 14159-14163 NN denotes Mcm4
T4609 14163-14164 HYPH denotes /
T4610 14164-14165 CD denotes 6
T4611 14165-14166 HYPH denotes /
T4612 14166-14167 CD denotes 7
T4607 14168-14175 NN denotes complex
T4613 14175-14177 , denotes ,
T4614 14177-14183 VBN denotes loaded
T4615 14184-14188 IN denotes onto
T4616 14189-14192 NN denotes DNA
T4617 14193-14195 IN denotes as
T4618 14196-14197 DT denotes a
T4620 14198-14204 JJ denotes double
T4621 14205-14214 JJ denotes hexameric
T4619 14215-14222 NN denotes complex
T4622 14222-14224 , denotes ,
T4623 14224-14235 RB denotes efficiently
T4605 14236-14243 VBZ denotes unwinds
T4624 14244-14247 DT denotes the
T4626 14248-14254 NN denotes duplex
T4625 14255-14261 NN denotes region
T4627 14262-14264 IN denotes of
T4628 14265-14268 DT denotes the
T4630 14269-14278 NN denotes substrate
T4629 14279-14282 NN denotes DNA
T4631 14282-14283 . denotes .
T4737 14285-14289 DT denotes Both
T4738 14290-14297 NNS denotes strands
T4740 14298-14300 IN denotes of
T4741 14301-14308 NN denotes thymine
T4743 14308-14309 HYPH denotes -
T4742 14309-14313 JJ denotes rich
T4745 14314-14320 JJ denotes single
T4747 14320-14321 HYPH denotes -
T4746 14321-14329 VBN denotes stranded
T4744 14330-14338 NNS denotes segments
T4748 14339-14342 VBP denotes are
T4739 14343-14351 VBN denotes required
T4749 14352-14355 IN denotes for
T4750 14356-14365 JJ denotes efficient
T4751 14366-14375 NN denotes unwinding
T4752 14376-14378 IN denotes of
T4753 14379-14385 NN denotes bubble
T4754 14386-14389 NN denotes DNA
T4755 14390-14392 IN denotes by
T4756 14393-14397 NN denotes Mcm4
T4757 14397-14398 HYPH denotes /
T4758 14398-14399 CD denotes 6
T4759 14399-14400 HYPH denotes /
T4760 14400-14401 CD denotes 7
T4761 14401-14542 sentence denotes Symmetric binding of the Mcm4/6/7 double hexamer to bubble suggests that each hexamer catalyzes unwinding of one of the two duplex segments.
T4762 14402-14411 JJ denotes Symmetric
T4763 14412-14419 NN denotes binding
T4765 14420-14422 IN denotes of
T4766 14423-14426 DT denotes the
T4768 14427-14431 NN denotes Mcm4
T4769 14431-14432 HYPH denotes /
T4770 14432-14433 CD denotes 6
T4771 14433-14434 HYPH denotes /
T4772 14434-14435 CD denotes 7
T4773 14436-14442 JJ denotes double
T4767 14443-14450 NN denotes hexamer
T4774 14451-14453 IN denotes to
T4775 14454-14460 NN denotes bubble
T4764 14461-14469 VBZ denotes suggests
T4776 14470-14474 IN denotes that
T4778 14475-14479 DT denotes each
T4779 14480-14487 NN denotes hexamer
T4777 14488-14497 VBZ denotes catalyzes
T4780 14498-14507 NN denotes unwinding
T4781 14508-14510 IN denotes of
T4782 14511-14514 CD denotes one
T4783 14515-14517 IN denotes of
T4784 14518-14521 DT denotes the
T4786 14522-14525 CD denotes two
T4787 14526-14532 NN denotes duplex
T4785 14533-14541 NNS denotes segments
T4788 14541-14542 . denotes .
T4789 14542-14638 sentence denotes We asked whether activation of both hexamers is required for unwinding of the bubble substrate.
T4790 14543-14545 PRP denotes We
T4791 14546-14551 VBD denotes asked
T4792 14552-14559 IN denotes whether
T4794 14560-14570 NN denotes activation
T4795 14571-14573 IN denotes of
T4796 14574-14578 DT denotes both
T4797 14579-14587 NNS denotes hexamers
T4798 14588-14590 VBZ denotes is
T4793 14591-14599 VBN denotes required
T4799 14600-14603 IN denotes for
T4800 14604-14613 NN denotes unwinding
T4801 14614-14616 IN denotes of
T4802 14617-14620 DT denotes the
T4804 14621-14627 NN denotes bubble
T4803 14628-14637 NN denotes substrate
T4805 14637-14638 . denotes .
T4806 14638-14785 sentence denotes The preferred bubble substrate which is efficiently unwound by Mcm4/6/7 contains thymine stretches on both strands of the single-stranded segment.
T4807 14639-14642 DT denotes The
T4809 14643-14652 JJ denotes preferred
T4810 14653-14659 NN denotes bubble
T4808 14660-14669 NN denotes substrate
T4812 14670-14675 WDT denotes which
T4814 14676-14678 VBZ denotes is
T4815 14679-14690 RB denotes efficiently
T4813 14691-14698 VBN denotes unwound
T4816 14699-14701 IN denotes by
T4817 14702-14706 NN denotes Mcm4
T4818 14706-14707 HYPH denotes /
T4819 14707-14708 CD denotes 6
T4820 14708-14709 HYPH denotes /
T4821 14709-14710 CD denotes 7
T4811 14711-14719 VBZ denotes contains
T4822 14720-14727 NN denotes thymine
T4823 14728-14737 NNS denotes stretches
T4824 14738-14740 IN denotes on
T4825 14741-14745 DT denotes both
T4826 14746-14753 NNS denotes strands
T4827 14754-14756 IN denotes of
T4828 14757-14760 DT denotes the
T4830 14761-14767 JJ denotes single
T4832 14767-14768 HYPH denotes -
T4831 14768-14776 VBN denotes stranded
T4829 14777-14784 NN denotes segment
T4833 14784-14785 . denotes .
T4834 14785-15017 sentence denotes We constructed a hetero-bubble (Bub66/T–G-rich) in which one strand is T-rich (same as Bub66/T-rich) and the other strand is G-rich (same as Bub66/G-rich) (13), and helicase assays were conducted with increasing amount of Mcm4/6/7.
T4835 14786-14788 PRP denotes We
T4836 14789-14800 VBD denotes constructed
T4837 14801-14802 DT denotes a
T4838 14803-14816 JJ denotes hetero-bubble
T4839 14817-14818 -LRB- denotes (
T4840 14818-14823 NN denotes Bub66
T4842 14823-14824 HYPH denotes /
T4843 14824-14825 NN denotes T
T4844 14825-14826 HYPH denotes –
T4841 14826-14827 NN denotes G
T4845 14827-14828 HYPH denotes -
T4846 14828-14832 JJ denotes rich
T4847 14832-14833 -RRB- denotes )
T4848 14834-14836 IN denotes in
T4850 14837-14842 WDT denotes which
T4851 14843-14846 CD denotes one
T4852 14847-14853 NN denotes strand
T4849 14854-14856 VBZ denotes is
T4853 14857-14858 NN denotes T
T4855 14858-14859 HYPH denotes -
T4854 14859-14863 JJ denotes rich
T4856 14864-14865 -LRB- denotes (
T4857 14865-14869 JJ denotes same
T4858 14870-14872 IN denotes as
T4859 14873-14878 NN denotes Bub66
T4861 14878-14879 HYPH denotes /
T4860 14879-14880 NN denotes T
T4862 14880-14881 HYPH denotes -
T4863 14881-14885 JJ denotes rich
T4864 14885-14886 -RRB- denotes )
T4865 14887-14890 CC denotes and
T4866 14891-14894 DT denotes the
T4868 14895-14900 JJ denotes other
T4867 14901-14907 NN denotes strand
T4869 14908-14910 VBZ denotes is
T4870 14911-14912 NN denotes G
T4872 14912-14913 HYPH denotes -
T4871 14913-14917 JJ denotes rich
T4873 14918-14919 -LRB- denotes (
T4874 14919-14923 JJ denotes same
T4875 14924-14926 IN denotes as
T4876 14927-14932 NN denotes Bub66
T4878 14932-14933 HYPH denotes /
T4877 14933-14934 NN denotes G
T4879 14934-14935 HYPH denotes -
T4880 14935-14939 JJ denotes rich
T4881 14939-14940 -RRB- denotes )
T4882 14941-14942 -LRB- denotes (
T4883 14942-14944 CD denotes 13
T4884 14944-14945 -RRB- denotes )
T4885 14945-14947 , denotes ,
T4886 14947-14950 CC denotes and
T4887 14951-14959 NN denotes helicase
T4888 14960-14966 NNS denotes assays
T4890 14967-14971 VBD denotes were
T4889 14972-14981 VBN denotes conducted
T4891 14982-14986 IN denotes with
T4892 14987-14997 VBG denotes increasing
T4893 14998-15004 NN denotes amount
T4894 15005-15007 IN denotes of
T4895 15008-15012 NN denotes Mcm4
T4896 15012-15013 HYPH denotes /
T4897 15013-15014 CD denotes 6
T4898 15014-15015 HYPH denotes /
T4899 15015-15016 CD denotes 7
T4900 15016-15017 . denotes .
T4901 15017-15106 sentence denotes Only weak unwinding was detected on this substrate compared to Bub66/T-rich (Figure 3A).
T4902 15018-15022 RB denotes Only
T4904 15023-15027 JJ denotes weak
T4903 15028-15037 NN denotes unwinding
T4906 15038-15041 VBD denotes was
T4905 15042-15050 VBN denotes detected
T4907 15051-15053 IN denotes on
T4908 15054-15058 DT denotes this
T4909 15059-15068 NN denotes substrate
T4910 15069-15077 VBN denotes compared
T4911 15078-15080 IN denotes to
T4912 15081-15086 NN denotes Bub66
T4914 15086-15087 HYPH denotes /
T4913 15087-15088 NN denotes T
T4915 15088-15089 HYPH denotes -
T4916 15089-15093 JJ denotes rich
T4917 15094-15095 -LRB- denotes (
T4919 15095-15101 NN denotes Figure
T4918 15102-15104 NN denotes 3A
T4920 15104-15105 -RRB- denotes )
T4921 15105-15106 . denotes .
T4922 15106-15297 sentence denotes Since Mcm4/6/7 can bind to Bub66/T–G-rich and forms a double hexameric complex (data not shown), the defective unwinding is not due to inefficient loading of the helicase onto the substrate.
T4923 15107-15112 IN denotes Since
T4925 15113-15117 NN denotes Mcm4
T4926 15117-15118 HYPH denotes /
T4927 15118-15119 CD denotes 6
T4928 15119-15120 HYPH denotes /
T4929 15120-15121 CD denotes 7
T4930 15122-15125 MD denotes can
T4924 15126-15130 VB denotes bind
T4932 15131-15133 IN denotes to
T4933 15134-15139 NN denotes Bub66
T4935 15139-15140 HYPH denotes /
T4936 15140-15141 NN denotes T
T4937 15141-15142 HYPH denotes –
T4934 15142-15143 NN denotes G
T4938 15143-15144 HYPH denotes -
T4939 15144-15148 JJ denotes rich
T4940 15149-15152 CC denotes and
T4941 15153-15158 VBZ denotes forms
T4942 15159-15160 DT denotes a
T4944 15161-15167 JJ denotes double
T4945 15168-15177 JJ denotes hexameric
T4943 15178-15185 NN denotes complex
T4946 15186-15187 -LRB- denotes (
T4948 15187-15191 NNS denotes data
T4949 15192-15195 RB denotes not
T4947 15196-15201 VBN denotes shown
T4950 15201-15202 -RRB- denotes )
T4951 15202-15204 , denotes ,
T4952 15204-15207 DT denotes the
T4954 15208-15217 JJ denotes defective
T4953 15218-15227 NN denotes unwinding
T4931 15228-15230 VBZ denotes is
T4955 15231-15234 RB denotes not
T4956 15235-15238 IN denotes due
T4957 15239-15241 IN denotes to
T4958 15242-15253 JJ denotes inefficient
T4959 15254-15261 NN denotes loading
T4960 15262-15264 IN denotes of
T4961 15265-15268 DT denotes the
T4962 15269-15277 NN denotes helicase
T4963 15278-15282 IN denotes onto
T4964 15283-15286 DT denotes the
T4965 15287-15296 NN denotes substrate
T4966 15296-15297 . denotes .
T4967 15297-15595 sentence denotes If one hexamer interacting with the thymine-rich strand dissociates from the other hexamer and displaces only one of the duplex segments, then it would result in generation of Y-fork structures which could not be further displaced due to the presence of G-rich 3′-tail and would appear on the gel.
T4968 15298-15300 IN denotes If
T4970 15301-15304 CD denotes one
T4971 15305-15312 NN denotes hexamer
T4972 15313-15324 VBG denotes interacting
T4973 15325-15329 IN denotes with
T4974 15330-15333 DT denotes the
T4976 15334-15341 NN denotes thymine
T4978 15341-15342 HYPH denotes -
T4977 15342-15346 JJ denotes rich
T4975 15347-15353 NN denotes strand
T4969 15354-15365 VBZ denotes dissociates
T4980 15366-15370 IN denotes from
T4981 15371-15374 DT denotes the
T4983 15375-15380 JJ denotes other
T4982 15381-15388 NN denotes hexamer
T4984 15389-15392 CC denotes and
T4985 15393-15402 VBZ denotes displaces
T4986 15403-15407 RB denotes only
T4987 15408-15411 CD denotes one
T4988 15412-15414 IN denotes of
T4989 15415-15418 DT denotes the
T4991 15419-15425 NN denotes duplex
T4990 15426-15434 NNS denotes segments
T4992 15434-15436 , denotes ,
T4993 15436-15440 RB denotes then
T4994 15441-15443 PRP denotes it
T4995 15444-15449 MD denotes would
T4979 15450-15456 VB denotes result
T4996 15457-15459 IN denotes in
T4997 15460-15470 NN denotes generation
T4998 15471-15473 IN denotes of
T4999 15474-15475 NN denotes Y
T5001 15475-15476 HYPH denotes -
T5000 15476-15480 NN denotes fork
T5002 15481-15491 NNS denotes structures
T5003 15492-15497 WDT denotes which
T5005 15498-15503 MD denotes could
T5006 15504-15507 RB denotes not
T5007 15508-15510 VB denotes be
T5008 15511-15518 RB denotes further
T5004 15519-15528 VBN denotes displaced
T5009 15529-15532 IN denotes due
T5010 15533-15535 IN denotes to
T5011 15536-15539 DT denotes the
T5012 15540-15548 NN denotes presence
T5013 15549-15551 IN denotes of
T5014 15552-15553 NN denotes G
T5016 15553-15554 HYPH denotes -
T5015 15554-15558 JJ denotes rich
T5018 15559-15560 CD denotes 3
T5019 15560-15561 SYM denotes ′
T5020 15561-15562 HYPH denotes -
T5017 15562-15566 NN denotes tail
T5021 15567-15570 CC denotes and
T5022 15571-15576 MD denotes would
T5023 15577-15583 VB denotes appear
T5024 15584-15586 IN denotes on
T5025 15587-15590 DT denotes the
T5026 15591-15594 NN denotes gel
T5027 15594-15595 . denotes .
T5028 15595-15821 sentence denotes However, we did not detect such intermediates during the course of the experiment (Figure 3B), suggesting that bubble can be displaced efficiently only when two molecules of the Mcm4/6/7 hexamers are simultaneously activated.
T5029 15596-15603 RB denotes However
T5031 15603-15605 , denotes ,
T5032 15605-15607 PRP denotes we
T5033 15608-15611 VBD denotes did
T5034 15612-15615 RB denotes not
T5030 15616-15622 VB denotes detect
T5035 15623-15627 JJ denotes such
T5036 15628-15641 NNS denotes intermediates
T5037 15642-15648 IN denotes during
T5038 15649-15652 DT denotes the
T5039 15653-15659 NN denotes course
T5040 15660-15662 IN denotes of
T5041 15663-15666 DT denotes the
T5042 15667-15677 NN denotes experiment
T5043 15678-15679 -LRB- denotes (
T5045 15679-15685 NN denotes Figure
T5044 15686-15688 NN denotes 3B
T5046 15688-15689 -RRB- denotes )
T5047 15689-15691 , denotes ,
T5048 15691-15701 VBG denotes suggesting
T5049 15702-15706 IN denotes that
T5051 15707-15713 NN denotes bubble
T5052 15714-15717 MD denotes can
T5053 15718-15720 VB denotes be
T5050 15721-15730 VBN denotes displaced
T5054 15731-15742 RB denotes efficiently
T5055 15743-15747 RB denotes only
T5057 15748-15752 WRB denotes when
T5058 15753-15756 CD denotes two
T5059 15757-15766 NNS denotes molecules
T5060 15767-15769 IN denotes of
T5061 15770-15773 DT denotes the
T5063 15774-15778 NN denotes Mcm4
T5064 15778-15779 HYPH denotes /
T5065 15779-15780 CD denotes 6
T5066 15780-15781 HYPH denotes /
T5067 15781-15782 CD denotes 7
T5062 15783-15791 NNS denotes hexamers
T5068 15792-15795 VBP denotes are
T5069 15796-15810 RB denotes simultaneously
T5056 15811-15820 VBN denotes activated
T5070 15820-15821 . denotes .
T5260 15823-15830 NN denotes Binding
T5262 15831-15834 CC denotes and
T5263 15835-15843 NN denotes helicase
T5261 15844-15851 NNS denotes actions
T5264 15852-15854 IN denotes of
T5265 15855-15859 NN denotes Mcm4
T5266 15859-15860 HYPH denotes /
T5267 15860-15861 CD denotes 6
T5268 15861-15862 HYPH denotes /
T5269 15862-15863 CD denotes 7
T5270 15864-15866 IN denotes on
T5271 15867-15873 VBN denotes forked
T5273 15874-15877 CC denotes and
T5274 15878-15879 CD denotes 3
T5276 15879-15880 SYM denotes ′
T5277 15880-15881 HYPH denotes -
T5278 15882-15884 CC denotes or
T5279 15885-15886 CD denotes 5
T5280 15886-15887 SYM denotes ′
T5281 15887-15888 HYPH denotes -
T5275 15888-15897 NN denotes extension
T5272 15898-15908 NNS denotes substrates
T5282 15908-16211 sentence denotes In order to examine more precisely the interaction and helicase actions with forked substrates, Y-fork substrates containing a nascent leading strand (A-fork[3′]), nascent lagging strand (A-fork[5′]), or both of them (A-fork[3′,5′]), as well as 5′-extension or 3′-extension structures were constructed.
T5283 15909-15911 IN denotes In
T5285 15912-15917 NN denotes order
T5286 15918-15920 TO denotes to
T5287 15921-15928 VB denotes examine
T5288 15929-15933 RBR denotes more
T5289 15934-15943 RB denotes precisely
T5290 15944-15947 DT denotes the
T5291 15948-15959 NN denotes interaction
T5292 15960-15963 CC denotes and
T5293 15964-15972 NN denotes helicase
T5294 15973-15980 NNS denotes actions
T5295 15981-15985 IN denotes with
T5296 15986-15992 VBN denotes forked
T5297 15993-16003 NNS denotes substrates
T5298 16003-16005 , denotes ,
T5299 16005-16006 NN denotes Y
T5301 16006-16007 HYPH denotes -
T5300 16007-16011 NN denotes fork
T5302 16012-16022 NNS denotes substrates
T5303 16023-16033 VBG denotes containing
T5304 16034-16035 DT denotes a
T5306 16036-16043 JJ denotes nascent
T5307 16044-16051 VBG denotes leading
T5305 16052-16058 NN denotes strand
T5308 16059-16060 -LRB- denotes (
T5309 16060-16061 NN denotes A
T5311 16061-16062 HYPH denotes -
T5310 16062-16066 NN denotes fork
T5312 16066-16067 -LRB- denotes [
T5313 16067-16068 CD denotes 3
T5314 16068-16069 SYM denotes ′
T5315 16069-16070 -RRB- denotes ]
T5316 16070-16071 -RRB- denotes )
T5317 16071-16073 , denotes ,
T5318 16073-16080 JJ denotes nascent
T5320 16081-16088 VBG denotes lagging
T5319 16089-16095 NN denotes strand
T5321 16096-16097 -LRB- denotes (
T5322 16097-16098 NN denotes A
T5324 16098-16099 HYPH denotes -
T5323 16099-16103 NN denotes fork
T5325 16103-16104 -LRB- denotes [
T5326 16104-16105 CD denotes 5
T5327 16105-16106 SYM denotes ′
T5328 16106-16107 -RRB- denotes ]
T5329 16107-16108 -RRB- denotes )
T5330 16108-16110 , denotes ,
T5331 16110-16112 CC denotes or
T5332 16113-16117 DT denotes both
T5333 16118-16120 IN denotes of
T5334 16121-16125 PRP denotes them
T5335 16126-16127 -LRB- denotes (
T5336 16127-16128 NN denotes A
T5338 16128-16129 HYPH denotes -
T5337 16129-16133 NN denotes fork
T5340 16133-16134 -LRB- denotes [
T5341 16134-16135 CD denotes 3
T5342 16135-16136 SYM denotes ′
T5343 16136-16137 , denotes ,
T5344 16137-16138 CD denotes 5
T5345 16138-16139 SYM denotes ′
T5346 16139-16140 -RRB- denotes ]
T5347 16140-16141 -RRB- denotes )
T5348 16141-16143 , denotes ,
T5349 16143-16145 RB denotes as
T5351 16146-16150 RB denotes well
T5350 16151-16153 IN denotes as
T5352 16154-16155 CD denotes 5
T5354 16155-16156 SYM denotes ′
T5355 16156-16157 HYPH denotes -
T5353 16157-16166 NN denotes extension
T5356 16167-16169 CC denotes or
T5357 16170-16171 CD denotes 3
T5359 16171-16172 SYM denotes ′
T5360 16172-16173 HYPH denotes -
T5358 16173-16182 NN denotes extension
T5339 16183-16193 NNS denotes structures
T5361 16194-16198 VBD denotes were
T5284 16199-16210 VBN denotes constructed
T5362 16210-16211 . denotes .
T5363 16211-16335 sentence denotes Mcm4/6/7 bound to A-fork[3′] or A-fork[5′] with affinity similar to that of a simple Y-fork (Figure 4A and data not shown).
T5364 16212-16216 NN denotes Mcm4
T5366 16216-16217 HYPH denotes /
T5367 16217-16218 CD denotes 6
T5368 16218-16219 HYPH denotes /
T5369 16219-16220 CD denotes 7
T5365 16221-16226 VBD denotes bound
T5370 16227-16229 IN denotes to
T5371 16230-16231 NN denotes A
T5373 16231-16232 HYPH denotes -
T5372 16232-16236 NN denotes fork
T5374 16236-16237 -LRB- denotes [
T5375 16237-16238 CD denotes 3
T5376 16238-16239 SYM denotes ′
T5377 16239-16240 -RRB- denotes ]
T5378 16241-16243 CC denotes or
T5379 16244-16245 NN denotes A
T5381 16245-16246 HYPH denotes -
T5380 16246-16250 NN denotes fork
T5382 16250-16251 -LRB- denotes [
T5383 16251-16252 CD denotes 5
T5384 16252-16253 SYM denotes ′
T5385 16253-16254 -RRB- denotes ]
T5386 16255-16259 IN denotes with
T5387 16260-16268 NN denotes affinity
T5388 16269-16276 JJ denotes similar
T5389 16277-16279 IN denotes to
T5390 16280-16284 DT denotes that
T5391 16285-16287 IN denotes of
T5392 16288-16289 DT denotes a
T5394 16290-16296 JJ denotes simple
T5395 16297-16298 NN denotes Y
T5396 16298-16299 HYPH denotes -
T5393 16299-16303 NN denotes fork
T5397 16304-16305 -LRB- denotes (
T5399 16305-16311 NN denotes Figure
T5398 16312-16314 NN denotes 4A
T5400 16315-16318 CC denotes and
T5401 16319-16323 NNS denotes data
T5403 16324-16327 RB denotes not
T5402 16328-16333 VBN denotes shown
T5404 16333-16334 -RRB- denotes )
T5405 16334-16335 . denotes .
T5406 16335-16471 sentence denotes In contrast, PriA helicase, used as a control, bound to A-fork[3′] more efficiently than to A-fork[5′], as reported previously (23,25).
T5407 16336-16338 IN denotes In
T5409 16339-16347 NN denotes contrast
T5410 16347-16349 , denotes ,
T5411 16349-16353 NN denotes PriA
T5412 16354-16362 NN denotes helicase
T5413 16362-16364 , denotes ,
T5414 16364-16368 VBN denotes used
T5415 16369-16371 IN denotes as
T5416 16372-16373 DT denotes a
T5417 16374-16381 NN denotes control
T5418 16381-16383 , denotes ,
T5408 16383-16388 VBD denotes bound
T5419 16389-16391 IN denotes to
T5420 16392-16393 NN denotes A
T5422 16393-16394 HYPH denotes -
T5421 16394-16398 NN denotes fork
T5423 16398-16399 -LRB- denotes [
T5424 16399-16400 CD denotes 3
T5425 16400-16401 SYM denotes ′
T5426 16401-16402 -RRB- denotes ]
T5427 16403-16407 RBR denotes more
T5428 16408-16419 RB denotes efficiently
T5429 16420-16424 IN denotes than
T5430 16425-16427 IN denotes to
T5431 16428-16429 NN denotes A
T5433 16429-16430 HYPH denotes -
T5432 16430-16434 NN denotes fork
T5434 16434-16435 -LRB- denotes [
T5435 16435-16436 CD denotes 5
T5436 16436-16437 SYM denotes ′
T5437 16437-16438 -RRB- denotes ]
T5438 16438-16440 , denotes ,
T5439 16440-16442 IN denotes as
T5440 16443-16451 VBN denotes reported
T5441 16452-16462 RB denotes previously
T5442 16463-16464 -LRB- denotes (
T5444 16464-16466 CD denotes 23
T5445 16466-16467 , denotes ,
T5443 16467-16469 CD denotes 25
T5446 16469-16470 -RRB- denotes )
T5447 16470-16471 . denotes .
T5448 16471-16614 sentence denotes Mcm4/6/7 bound also to 5′-extension and 3′-extension, albeit with slightly reduced affinity compared to A-fork[3′] and A-fork[5′] (Figure 4A).
T5449 16472-16476 NN denotes Mcm4
T5451 16476-16477 HYPH denotes /
T5452 16477-16478 CD denotes 6
T5453 16478-16479 HYPH denotes /
T5454 16479-16480 CD denotes 7
T5450 16481-16486 VBD denotes bound
T5455 16487-16491 RB denotes also
T5456 16492-16494 IN denotes to
T5457 16495-16496 CD denotes 5
T5459 16496-16497 SYM denotes ′
T5460 16497-16498 HYPH denotes -
T5458 16498-16507 NN denotes extension
T5461 16508-16511 CC denotes and
T5462 16512-16513 CD denotes 3
T5464 16513-16514 SYM denotes ′
T5465 16514-16515 HYPH denotes -
T5463 16515-16524 NN denotes extension
T5466 16524-16526 , denotes ,
T5467 16526-16532 IN denotes albeit
T5468 16533-16537 IN denotes with
T5469 16538-16546 RB denotes slightly
T5470 16547-16554 VBN denotes reduced
T5471 16555-16563 NN denotes affinity
T5472 16564-16572 VBN denotes compared
T5473 16573-16575 IN denotes to
T5474 16576-16577 NN denotes A
T5476 16577-16578 HYPH denotes -
T5475 16578-16582 NN denotes fork
T5477 16582-16583 -LRB- denotes [
T5478 16583-16584 CD denotes 3
T5479 16584-16585 SYM denotes ′
T5480 16585-16586 -RRB- denotes ]
T5481 16587-16590 CC denotes and
T5482 16591-16592 NN denotes A
T5484 16592-16593 HYPH denotes -
T5483 16593-16597 NN denotes fork
T5485 16597-16598 -LRB- denotes [
T5486 16598-16599 CD denotes 5
T5487 16599-16600 SYM denotes ′
T5488 16600-16601 -RRB- denotes ]
T5489 16602-16603 -LRB- denotes (
T5491 16603-16609 NN denotes Figure
T5490 16610-16612 NN denotes 4A
T5492 16612-16613 -RRB- denotes )
T5493 16613-16614 . denotes .
T5494 16614-16716 sentence denotes Mcm4/6/7 bound to A-fork[3′,5′] with much lower affinity, and the complex gave rise to a single band.
T5495 16615-16619 NN denotes Mcm4
T5497 16619-16620 HYPH denotes /
T5498 16620-16621 CD denotes 6
T5499 16621-16622 HYPH denotes /
T5500 16622-16623 CD denotes 7
T5496 16624-16629 VBD denotes bound
T5501 16630-16632 IN denotes to
T5502 16633-16634 NN denotes A
T5504 16634-16635 HYPH denotes -
T5503 16635-16639 NN denotes fork
T5505 16639-16640 -LRB- denotes [
T5506 16640-16641 CD denotes 3
T5507 16641-16642 SYM denotes ′
T5508 16642-16643 , denotes ,
T5509 16643-16644 CD denotes 5
T5510 16644-16645 SYM denotes ′
T5511 16645-16646 -RRB- denotes ]
T5512 16647-16651 IN denotes with
T5513 16652-16656 RB denotes much
T5514 16657-16662 JJR denotes lower
T5515 16663-16671 NN denotes affinity
T5516 16671-16673 , denotes ,
T5517 16673-16676 CC denotes and
T5518 16677-16680 DT denotes the
T5519 16681-16688 NN denotes complex
T5520 16689-16693 VBD denotes gave
T5521 16694-16698 NN denotes rise
T5522 16699-16701 IN denotes to
T5523 16702-16703 DT denotes a
T5525 16704-16710 JJ denotes single
T5524 16711-16715 NN denotes band
T5526 16715-16716 . denotes .
T5527 16716-16898 sentence denotes These findings indicate that the Mcm4/6/7 complex is loaded onto DNA preferentially and efficiently through single-stranded region, regardless of the presence of 3′- or 5′-terminus.
T5528 16717-16722 DT denotes These
T5529 16723-16731 NNS denotes findings
T5530 16732-16740 VBP denotes indicate
T5531 16741-16745 IN denotes that
T5533 16746-16749 DT denotes the
T5535 16750-16754 NN denotes Mcm4
T5536 16754-16755 HYPH denotes /
T5537 16755-16756 CD denotes 6
T5538 16756-16757 HYPH denotes /
T5539 16757-16758 CD denotes 7
T5534 16759-16766 NN denotes complex
T5540 16767-16769 VBZ denotes is
T5532 16770-16776 VBN denotes loaded
T5541 16777-16781 IN denotes onto
T5542 16782-16785 NN denotes DNA
T5543 16786-16800 RB denotes preferentially
T5544 16801-16804 CC denotes and
T5545 16805-16816 RB denotes efficiently
T5546 16817-16824 IN denotes through
T5547 16825-16831 JJ denotes single
T5549 16831-16832 HYPH denotes -
T5548 16832-16840 VBN denotes stranded
T5550 16841-16847 NN denotes region
T5551 16847-16849 , denotes ,
T5552 16849-16859 RB denotes regardless
T5553 16860-16862 IN denotes of
T5554 16863-16866 DT denotes the
T5555 16867-16875 NN denotes presence
T5556 16876-16878 IN denotes of
T5557 16879-16880 CD denotes 3
T5559 16880-16881 SYM denotes ′
T5560 16881-16882 HYPH denotes -
T5561 16883-16885 CC denotes or
T5562 16886-16887 CD denotes 5
T5563 16887-16888 SYM denotes ′
T5564 16888-16889 HYPH denotes -
T5558 16889-16897 NN denotes terminus
T5565 16897-16898 . denotes .
T5566 16898-17082 sentence denotes In contrast, only the substrate containing a 3′ single-stranded DNA tail (A-fork[5′]) was efficiently unwound and A-fork[3′] or A-fork[3′,5′], was not unwound by Mcm4/6/7 (Figure 4B).
T5567 16899-16901 IN denotes In
T5569 16902-16910 NN denotes contrast
T5570 16910-16912 , denotes ,
T5571 16912-16916 RB denotes only
T5573 16917-16920 DT denotes the
T5572 16921-16930 NN denotes substrate
T5574 16931-16941 VBG denotes containing
T5575 16942-16943 DT denotes a
T5577 16944-16945 CD denotes 3
T5578 16945-16946 SYM denotes ′
T5579 16947-16953 JJ denotes single
T5581 16953-16954 HYPH denotes -
T5580 16954-16962 VBN denotes stranded
T5582 16963-16966 NN denotes DNA
T5576 16967-16971 NN denotes tail
T5583 16972-16973 -LRB- denotes (
T5585 16973-16974 NN denotes A
T5586 16974-16975 HYPH denotes -
T5584 16975-16979 NN denotes fork
T5587 16979-16980 -LRB- denotes [
T5588 16980-16981 CD denotes 5
T5589 16981-16982 SYM denotes ′
T5590 16982-16983 -RRB- denotes ]
T5591 16983-16984 -RRB- denotes )
T5592 16985-16988 VBD denotes was
T5593 16989-17000 RB denotes efficiently
T5568 17001-17008 VBN denotes unwound
T5594 17009-17012 CC denotes and
T5595 17013-17014 NN denotes A
T5597 17014-17015 HYPH denotes -
T5596 17015-17019 NN denotes fork
T5599 17019-17020 -LRB- denotes [
T5600 17020-17021 CD denotes 3
T5601 17021-17022 SYM denotes ′
T5602 17022-17023 -RRB- denotes ]
T5603 17024-17026 CC denotes or
T5604 17027-17028 NN denotes A
T5606 17028-17029 HYPH denotes -
T5605 17029-17033 NN denotes fork
T5607 17033-17034 -LRB- denotes [
T5608 17034-17035 CD denotes 3
T5609 17035-17036 SYM denotes ′
T5610 17036-17037 , denotes ,
T5611 17037-17038 CD denotes 5
T5612 17038-17039 SYM denotes ′
T5613 17039-17040 -RRB- denotes ]
T5614 17040-17042 , denotes ,
T5615 17042-17045 VBD denotes was
T5616 17046-17049 RB denotes not
T5598 17050-17057 VBN denotes unwound
T5617 17058-17060 IN denotes by
T5618 17061-17065 NN denotes Mcm4
T5619 17065-17066 HYPH denotes /
T5620 17066-17067 CD denotes 6
T5621 17067-17068 HYPH denotes /
T5622 17068-17069 CD denotes 7
T5623 17070-17071 -LRB- denotes (
T5625 17071-17077 NN denotes Figure
T5624 17078-17080 NN denotes 4B
T5626 17080-17081 -RRB- denotes )
T5627 17081-17082 . denotes .
T5628 17082-17259 sentence denotes Absence of helicase actions on A-fork[3′,5′] composed only of duplex regions is expected since double-stranded DNA is not able to activate the ATPase activity of Mcm4/6/7 (10).
T5629 17083-17090 NN denotes Absence
T5631 17091-17093 IN denotes of
T5632 17094-17102 NN denotes helicase
T5633 17103-17110 NNS denotes actions
T5634 17111-17113 IN denotes on
T5635 17114-17115 NN denotes A
T5637 17115-17116 HYPH denotes -
T5636 17116-17120 NN denotes fork
T5638 17120-17121 -LRB- denotes [
T5639 17121-17122 CD denotes 3
T5640 17122-17123 SYM denotes ′
T5641 17123-17124 , denotes ,
T5642 17124-17125 CD denotes 5
T5643 17125-17126 SYM denotes ′
T5644 17126-17127 -RRB- denotes ]
T5645 17128-17136 VBN denotes composed
T5646 17137-17141 RB denotes only
T5647 17142-17144 IN denotes of
T5648 17145-17151 NN denotes duplex
T5649 17152-17159 NNS denotes regions
T5650 17160-17162 VBZ denotes is
T5630 17163-17171 VBN denotes expected
T5651 17172-17177 IN denotes since
T5653 17178-17184 JJ denotes double
T5655 17184-17185 HYPH denotes -
T5654 17185-17193 VBN denotes stranded
T5656 17194-17197 NN denotes DNA
T5652 17198-17200 VBZ denotes is
T5657 17201-17204 RB denotes not
T5658 17205-17209 JJ denotes able
T5659 17210-17212 TO denotes to
T5660 17213-17221 VB denotes activate
T5661 17222-17225 DT denotes the
T5663 17226-17232 NN denotes ATPase
T5662 17233-17241 NN denotes activity
T5664 17242-17244 IN denotes of
T5665 17245-17249 NN denotes Mcm4
T5666 17249-17250 HYPH denotes /
T5667 17250-17251 CD denotes 6
T5668 17251-17252 HYPH denotes /
T5669 17252-17253 CD denotes 7
T5670 17254-17255 -LRB- denotes (
T5671 17255-17257 CD denotes 10
T5672 17257-17258 -RRB- denotes )
T5673 17258-17259 . denotes .
T5674 17259-17510 sentence denotes In contrast, the PriA, a structure-specific helicase used as a control, binds at the fork junction and unwinds the fork and the nascent lagging strand DNA on A-fork[5′], or unwinds the nascent lagging strand on A-fork[3′,5′], as reported before (25).
T5675 17260-17262 IN denotes In
T5677 17263-17271 NN denotes contrast
T5678 17271-17273 , denotes ,
T5679 17273-17276 DT denotes the
T5680 17277-17281 NN denotes PriA
T5681 17281-17283 , denotes ,
T5682 17283-17284 DT denotes a
T5684 17285-17294 NN denotes structure
T5686 17294-17295 HYPH denotes -
T5685 17295-17303 JJ denotes specific
T5683 17304-17312 NN denotes helicase
T5687 17313-17317 VBN denotes used
T5688 17318-17320 IN denotes as
T5689 17321-17322 DT denotes a
T5690 17323-17330 NN denotes control
T5691 17330-17332 , denotes ,
T5676 17332-17337 VBZ denotes binds
T5692 17338-17340 IN denotes at
T5693 17341-17344 DT denotes the
T5695 17345-17349 NN denotes fork
T5694 17350-17358 NN denotes junction
T5696 17359-17362 CC denotes and
T5697 17363-17370 VBZ denotes unwinds
T5698 17371-17374 DT denotes the
T5699 17375-17379 NN denotes fork
T5700 17380-17383 CC denotes and
T5701 17384-17387 DT denotes the
T5703 17388-17395 JJ denotes nascent
T5704 17396-17403 VBG denotes lagging
T5705 17404-17410 NN denotes strand
T5702 17411-17414 NN denotes DNA
T5706 17415-17417 IN denotes on
T5707 17418-17419 NN denotes A
T5709 17419-17420 HYPH denotes -
T5708 17420-17424 NN denotes fork
T5710 17424-17425 -LRB- denotes [
T5711 17425-17426 CD denotes 5
T5712 17426-17427 SYM denotes ′
T5713 17427-17428 -RRB- denotes ]
T5714 17428-17430 , denotes ,
T5715 17430-17432 CC denotes or
T5716 17433-17440 VBZ denotes unwinds
T5717 17441-17444 DT denotes the
T5719 17445-17452 JJ denotes nascent
T5720 17453-17460 VBG denotes lagging
T5718 17461-17467 NN denotes strand
T5721 17468-17470 IN denotes on
T5722 17471-17472 NN denotes A
T5724 17472-17473 HYPH denotes -
T5723 17473-17477 NN denotes fork
T5725 17477-17478 -LRB- denotes [
T5726 17478-17479 CD denotes 3
T5727 17479-17480 SYM denotes ′
T5728 17480-17481 , denotes ,
T5729 17481-17482 CD denotes 5
T5730 17482-17483 SYM denotes ′
T5731 17483-17484 -RRB- denotes ]
T5732 17484-17486 , denotes ,
T5733 17486-17488 IN denotes as
T5734 17489-17497 VBN denotes reported
T5735 17498-17504 RB denotes before
T5736 17505-17506 -LRB- denotes (
T5737 17506-17508 CD denotes 25
T5738 17508-17509 -RRB- denotes )
T5739 17509-17510 . denotes .
T5740 17510-17674 sentence denotes These results indicate that the Mcm4/6/7 complex binds to single-stranded DNA and that its helicase is activated only in the presence of a single-stranded 3′-tail.
T5741 17511-17516 DT denotes These
T5742 17517-17524 NNS denotes results
T5743 17525-17533 VBP denotes indicate
T5744 17534-17538 IN denotes that
T5746 17539-17542 DT denotes the
T5748 17543-17547 NN denotes Mcm4
T5749 17547-17548 HYPH denotes /
T5750 17548-17549 CD denotes 6
T5751 17549-17550 HYPH denotes /
T5752 17550-17551 CD denotes 7
T5747 17552-17559 NN denotes complex
T5745 17560-17565 VBZ denotes binds
T5753 17566-17568 IN denotes to
T5754 17569-17575 JJ denotes single
T5756 17575-17576 HYPH denotes -
T5755 17576-17584 VBN denotes stranded
T5757 17585-17588 NN denotes DNA
T5758 17589-17592 CC denotes and
T5759 17593-17597 IN denotes that
T5761 17598-17601 PRP$ denotes its
T5762 17602-17610 NN denotes helicase
T5763 17611-17613 VBZ denotes is
T5760 17614-17623 VBN denotes activated
T5764 17624-17628 RB denotes only
T5765 17629-17631 IN denotes in
T5766 17632-17635 DT denotes the
T5767 17636-17644 NN denotes presence
T5768 17645-17647 IN denotes of
T5769 17648-17649 DT denotes a
T5771 17650-17656 JJ denotes single
T5773 17656-17657 HYPH denotes -
T5772 17657-17665 VBN denotes stranded
T5774 17666-17667 CD denotes 3
T5775 17667-17668 SYM denotes ′
T5776 17668-17669 HYPH denotes -
T5770 17669-17673 NN denotes tail
T5777 17673-17674 . denotes .
T5933 17676-17677 CD denotes 3
T5935 17677-17678 SYM denotes ′
T5936 17678-17679 HYPH denotes -
T5934 17679-17688 NN denotes extension
T5938 17689-17693 IN denotes with
T5939 17694-17698 NN denotes poly
T5941 17698-17699 -LRB- denotes (
T5940 17699-17701 NN denotes dT
T5943 17701-17702 -RRB- denotes )
T5942 17703-17707 NN denotes tail
T5944 17708-17710 VBZ denotes is
T5937 17711-17720 VBN denotes displaced
T5945 17721-17723 IN denotes by
T5946 17724-17728 NN denotes Mcm4
T5947 17728-17729 HYPH denotes /
T5948 17729-17730 CD denotes 6
T5949 17730-17731 HYPH denotes /
T5950 17731-17732 CD denotes 7
T5951 17732-17885 sentence denotes It has been reported that the 3′-extension can be unwound by the archaeal Mcm homohexamer but not by fission and budding yeast Mcm complexes (14,26,27).
T5952 17733-17735 PRP denotes It
T5954 17736-17739 VBZ denotes has
T5955 17740-17744 VBN denotes been
T5953 17745-17753 VBN denotes reported
T5956 17754-17758 IN denotes that
T5958 17759-17762 DT denotes the
T5960 17763-17764 CD denotes 3
T5961 17764-17765 SYM denotes ′
T5962 17765-17766 HYPH denotes -
T5959 17766-17775 NN denotes extension
T5963 17776-17779 MD denotes can
T5964 17780-17782 VB denotes be
T5957 17783-17790 JJ denotes unwound
T5965 17791-17793 IN denotes by
T5966 17794-17797 DT denotes the
T5968 17798-17806 JJ denotes archaeal
T5969 17807-17810 NN denotes Mcm
T5967 17811-17822 NN denotes homohexamer
T5970 17823-17826 CC denotes but
T5971 17827-17830 RB denotes not
T5972 17831-17833 IN denotes by
T5973 17834-17841 NN denotes fission
T5975 17842-17845 CC denotes and
T5976 17846-17853 NN denotes budding
T5977 17854-17859 NN denotes yeast
T5978 17860-17863 NN denotes Mcm
T5974 17864-17873 NNS denotes complexes
T5979 17874-17875 -LRB- denotes (
T5981 17875-17877 CD denotes 14
T5982 17877-17878 , denotes ,
T5983 17878-17880 CD denotes 26
T5984 17880-17881 , denotes ,
T5980 17881-17883 CD denotes 27
T5985 17883-17884 -RRB- denotes )
T5986 17884-17885 . denotes .
T5987 17885-18065 sentence denotes We have constructed 3′-extension substrates containing poly(dT), poly(dA), poly(dC) and poly(dG) 3′-tails in order to examine whether mouse Mcm4/6/7 can displace these substrates.
T5988 17886-17888 PRP denotes We
T5990 17889-17893 VBP denotes have
T5989 17894-17905 VBN denotes constructed
T5991 17906-17907 CD denotes 3
T5993 17907-17908 SYM denotes ′
T5994 17908-17909 HYPH denotes -
T5992 17909-17918 NN denotes extension
T5995 17919-17929 NNS denotes substrates
T5996 17930-17940 VBG denotes containing
T5997 17941-17945 NN denotes poly
T5999 17945-17946 -LRB- denotes (
T5998 17946-17948 NN denotes dT
T6001 17948-17949 -RRB- denotes )
T6002 17949-17951 , denotes ,
T6003 17951-17955 NN denotes poly
T6005 17955-17956 -LRB- denotes (
T6004 17956-17958 NN denotes dA
T6006 17958-17959 -RRB- denotes )
T6007 17959-17961 , denotes ,
T6008 17961-17965 NN denotes poly
T6010 17965-17966 -LRB- denotes (
T6009 17966-17968 NN denotes dC
T6011 17968-17969 -RRB- denotes )
T6012 17970-17973 CC denotes and
T6013 17974-17978 NN denotes poly
T6015 17978-17979 -LRB- denotes (
T6014 17979-17981 NN denotes dG
T6016 17981-17982 -RRB- denotes )
T6017 17983-17984 CD denotes 3
T6018 17984-17985 SYM denotes ′
T6019 17985-17986 HYPH denotes -
T6000 17986-17991 NNS denotes tails
T6020 17992-17994 IN denotes in
T6021 17995-18000 NN denotes order
T6022 18001-18003 TO denotes to
T6023 18004-18011 VB denotes examine
T6024 18012-18019 IN denotes whether
T6026 18020-18025 NN denotes mouse
T6027 18026-18030 NN denotes Mcm4
T6028 18030-18031 HYPH denotes /
T6029 18031-18032 CD denotes 6
T6030 18032-18033 HYPH denotes /
T6031 18033-18034 CD denotes 7
T6032 18035-18038 MD denotes can
T6025 18039-18047 VB denotes displace
T6033 18048-18053 DT denotes these
T6034 18054-18064 NNS denotes substrates
T6035 18064-18065 . denotes .
T6036 18065-18216 sentence denotes We first found that Mcm4/6/7 binds to dT-, dC- and dG-3′-extension with similar affinity, and to dA-3′-extension with much lower affinity (Figure 5A).
T6037 18066-18068 PRP denotes We
T6039 18069-18074 RB denotes first
T6038 18075-18080 VBD denotes found
T6040 18081-18085 IN denotes that
T6042 18086-18090 NN denotes Mcm4
T6043 18090-18091 HYPH denotes /
T6044 18091-18092 CD denotes 6
T6045 18092-18093 HYPH denotes /
T6046 18093-18094 CD denotes 7
T6041 18095-18100 VBZ denotes binds
T6047 18101-18103 IN denotes to
T6048 18104-18106 NN denotes dT
T6050 18106-18107 HYPH denotes -
T6051 18107-18109 , denotes ,
T6052 18109-18111 NN denotes dC
T6053 18111-18112 HYPH denotes -
T6054 18113-18116 CC denotes and
T6055 18117-18119 NN denotes dG
T6056 18119-18120 HYPH denotes -
T6057 18120-18121 CD denotes 3
T6058 18121-18122 SYM denotes ′
T6059 18122-18123 HYPH denotes -
T6049 18123-18132 NN denotes extension
T6060 18133-18137 IN denotes with
T6061 18138-18145 JJ denotes similar
T6062 18146-18154 NN denotes affinity
T6063 18154-18156 , denotes ,
T6064 18156-18159 CC denotes and
T6065 18160-18162 IN denotes to
T6066 18163-18165 NN denotes dA
T6068 18165-18166 HYPH denotes -
T6069 18166-18167 CD denotes 3
T6070 18167-18168 SYM denotes ′
T6071 18168-18169 HYPH denotes -
T6067 18169-18178 NN denotes extension
T6072 18179-18183 IN denotes with
T6073 18184-18188 RB denotes much
T6074 18189-18194 JJR denotes lower
T6075 18195-18203 NN denotes affinity
T6076 18204-18205 -LRB- denotes (
T6078 18205-18211 NN denotes Figure
T6077 18212-18214 NN denotes 5A
T6079 18214-18215 -RRB- denotes )
T6080 18215-18216 . denotes .
T6081 18216-18347 sentence denotes This is consistent with the previous results that Mcm4/6/7 binds to 50mer oligo-dT, -dC and -dG, but not to a 50mer oligo-dA (13).
T6082 18217-18221 DT denotes This
T6083 18222-18224 VBZ denotes is
T6084 18225-18235 JJ denotes consistent
T6085 18236-18240 IN denotes with
T6086 18241-18244 DT denotes the
T6088 18245-18253 JJ denotes previous
T6087 18254-18261 NNS denotes results
T6089 18262-18266 IN denotes that
T6091 18267-18271 NN denotes Mcm4
T6092 18271-18272 HYPH denotes /
T6093 18272-18273 CD denotes 6
T6094 18273-18274 HYPH denotes /
T6095 18274-18275 CD denotes 7
T6090 18276-18281 VBZ denotes binds
T6096 18282-18284 IN denotes to
T6097 18285-18290 NN denotes 50mer
T6099 18291-18296 NN denotes oligo
T6100 18296-18297 HYPH denotes -
T6098 18297-18299 NN denotes dT
T6101 18299-18301 , denotes ,
T6102 18301-18302 HYPH denotes -
T6103 18302-18304 NN denotes dC
T6104 18305-18308 CC denotes and
T6105 18309-18310 HYPH denotes -
T6106 18310-18312 NN denotes dG
T6107 18312-18314 , denotes ,
T6108 18314-18317 CC denotes but
T6109 18318-18321 RB denotes not
T6110 18322-18324 IN denotes to
T6111 18325-18326 DT denotes a
T6113 18327-18332 NN denotes 50mer
T6114 18333-18338 NN denotes oligo
T6115 18338-18339 HYPH denotes -
T6112 18339-18341 NN denotes dA
T6116 18342-18343 -LRB- denotes (
T6117 18343-18345 CD denotes 13
T6118 18345-18346 -RRB- denotes )
T6119 18346-18347 . denotes .
T6120 18347-18515 sentence denotes We found that dT-tailed 3′-extension was displaced efficiently by Mcm 4/6/7 while almost no or very little activity was detected on other substrates (Figure 5B and C).
T6121 18348-18350 PRP denotes We
T6122 18351-18356 VBD denotes found
T6123 18357-18361 IN denotes that
T6125 18362-18364 NN denotes dT
T6127 18364-18365 HYPH denotes -
T6126 18365-18371 VBN denotes tailed
T6129 18372-18373 CD denotes 3
T6130 18373-18374 SYM denotes ′
T6131 18374-18375 HYPH denotes -
T6128 18375-18384 NN denotes extension
T6132 18385-18388 VBD denotes was
T6124 18389-18398 VBN denotes displaced
T6133 18399-18410 RB denotes efficiently
T6134 18411-18413 IN denotes by
T6135 18414-18417 NN denotes Mcm
T6136 18418-18419 CD denotes 4
T6137 18419-18420 HYPH denotes /
T6138 18420-18421 CD denotes 6
T6139 18421-18422 HYPH denotes /
T6140 18422-18423 CD denotes 7
T6141 18424-18429 IN denotes while
T6143 18430-18436 RB denotes almost
T6144 18437-18439 DT denotes no
T6145 18440-18442 CC denotes or
T6146 18443-18447 RB denotes very
T6147 18448-18454 JJ denotes little
T6148 18455-18463 NN denotes activity
T6149 18464-18467 VBD denotes was
T6142 18468-18476 VBN denotes detected
T6150 18477-18479 IN denotes on
T6151 18480-18485 JJ denotes other
T6152 18486-18496 NNS denotes substrates
T6153 18497-18498 -LRB- denotes (
T6155 18498-18504 NN denotes Figure
T6154 18505-18507 NN denotes 5B
T6156 18508-18511 CC denotes and
T6157 18512-18513 NN denotes C
T6158 18513-18514 -RRB- denotes )
T6159 18514-18515 . denotes .
T6160 18515-18629 sentence denotes This is in sharp contrast to yeast Mcm4/6/7 helicases which are not capable of unwinding 3′-extension substrates.
T6161 18516-18520 DT denotes This
T6162 18521-18523 VBZ denotes is
T6163 18524-18526 IN denotes in
T6164 18527-18532 JJ denotes sharp
T6165 18533-18541 NN denotes contrast
T6166 18542-18544 IN denotes to
T6167 18545-18550 NN denotes yeast
T6169 18551-18555 NN denotes Mcm4
T6170 18555-18556 HYPH denotes /
T6171 18556-18557 CD denotes 6
T6172 18557-18558 HYPH denotes /
T6173 18558-18559 CD denotes 7
T6168 18560-18569 NNS denotes helicases
T6174 18570-18575 WDT denotes which
T6175 18576-18579 VBP denotes are
T6176 18580-18583 RB denotes not
T6177 18584-18591 JJ denotes capable
T6178 18592-18594 IN denotes of
T6179 18595-18604 VBG denotes unwinding
T6180 18605-18606 CD denotes 3
T6182 18606-18607 SYM denotes ′
T6183 18607-18608 HYPH denotes -
T6181 18608-18617 NN denotes extension
T6184 18618-18628 NNS denotes substrates
T6185 18628-18629 . denotes .
T6186 18629-18735 sentence denotes Furthermore, dT-tailed 5′-extension, to which Mcm4/6/7 could bind, was not displaced by the Mcm helicase.
T6187 18630-18641 RB denotes Furthermore
T6189 18641-18643 , denotes ,
T6190 18643-18645 NN denotes dT
T6192 18645-18646 HYPH denotes -
T6191 18646-18652 VBN denotes tailed
T6194 18653-18654 CD denotes 5
T6195 18654-18655 SYM denotes ′
T6196 18655-18656 HYPH denotes -
T6193 18656-18665 NN denotes extension
T6197 18665-18667 , denotes ,
T6198 18667-18669 IN denotes to
T6200 18670-18675 WDT denotes which
T6201 18676-18680 NN denotes Mcm4
T6202 18680-18681 HYPH denotes /
T6203 18681-18682 CD denotes 6
T6204 18682-18683 HYPH denotes /
T6205 18683-18684 CD denotes 7
T6206 18685-18690 MD denotes could
T6199 18691-18695 VB denotes bind
T6207 18695-18697 , denotes ,
T6208 18697-18700 VBD denotes was
T6209 18701-18704 RB denotes not
T6188 18705-18714 VBN denotes displaced
T6210 18715-18717 IN denotes by
T6211 18718-18721 DT denotes the
T6213 18722-18725 NN denotes Mcm
T6212 18726-18734 NN denotes helicase
T6214 18734-18735 . denotes .
T6215 18735-18926 sentence denotes The addition of the 5′-tail to dT-tailed 3′-extension further stimulated the displacement (Figure 5D), confirming the previous report that the fork structures facilitate the helicase action.
T6216 18736-18739 DT denotes The
T6217 18740-18748 NN denotes addition
T6219 18749-18751 IN denotes of
T6220 18752-18755 DT denotes the
T6222 18756-18757 CD denotes 5
T6223 18757-18758 SYM denotes ′
T6224 18758-18759 HYPH denotes -
T6221 18759-18763 NN denotes tail
T6225 18764-18766 IN denotes to
T6226 18767-18769 NN denotes dT
T6228 18769-18770 HYPH denotes -
T6227 18770-18776 VBN denotes tailed
T6230 18777-18778 CD denotes 3
T6231 18778-18779 SYM denotes ′
T6232 18779-18780 HYPH denotes -
T6229 18780-18789 NN denotes extension
T6233 18790-18797 RB denotes further
T6218 18798-18808 VBD denotes stimulated
T6234 18809-18812 DT denotes the
T6235 18813-18825 NN denotes displacement
T6236 18826-18827 -LRB- denotes (
T6238 18827-18833 NN denotes Figure
T6237 18834-18836 NN denotes 5D
T6239 18836-18837 -RRB- denotes )
T6240 18837-18839 , denotes ,
T6241 18839-18849 VBG denotes confirming
T6242 18850-18853 DT denotes the
T6244 18854-18862 JJ denotes previous
T6243 18863-18869 NN denotes report
T6245 18870-18874 IN denotes that
T6247 18875-18878 DT denotes the
T6249 18879-18883 NN denotes fork
T6248 18884-18894 NNS denotes structures
T6246 18895-18905 VBP denotes facilitate
T6250 18906-18909 DT denotes the
T6252 18910-18918 NN denotes helicase
T6251 18919-18925 NN denotes action
T6253 18925-18926 . denotes .
T6254 18926-19090 sentence denotes Thus, these results further strengthen our conclusion that the mammalian Mcm helicase is generally activated by the thymine-stretch on the 3′-single-stranded tail.
T6255 18927-18931 RB denotes Thus
T6257 18931-18933 , denotes ,
T6258 18933-18938 DT denotes these
T6259 18939-18946 NNS denotes results
T6260 18947-18954 RB denotes further
T6256 18955-18965 VB denotes strengthen
T6261 18966-18969 PRP$ denotes our
T6262 18970-18980 NN denotes conclusion
T6263 18981-18985 IN denotes that
T6265 18986-18989 DT denotes the
T6267 18990-18999 JJ denotes mammalian
T6268 19000-19003 NN denotes Mcm
T6266 19004-19012 NN denotes helicase
T6269 19013-19015 VBZ denotes is
T6270 19016-19025 RB denotes generally
T6264 19026-19035 VBN denotes activated
T6271 19036-19038 IN denotes by
T6272 19039-19042 DT denotes the
T6274 19043-19050 NN denotes thymine
T6275 19050-19051 HYPH denotes -
T6273 19051-19058 NN denotes stretch
T6276 19059-19061 IN denotes on
T6277 19062-19065 DT denotes the
T6279 19066-19067 CD denotes 3
T6280 19067-19068 SYM denotes ′
T6281 19068-19069 HYPH denotes -
T6282 19069-19075 JJ denotes single
T6284 19075-19076 HYPH denotes -
T6283 19076-19084 VBN denotes stranded
T6278 19085-19089 NN denotes tail
T6285 19089-19090 . denotes .
T6404 19092-19095 DT denotes The
T6405 19096-19103 NNS denotes effects
T6406 19104-19106 IN denotes of
T6407 19107-19110 DT denotes the
T6409 19111-19118 NN denotes thymine
T6408 19119-19126 NN denotes content
T6410 19127-19129 IN denotes of
T6411 19130-19133 DT denotes the
T6413 19134-19135 CD denotes 3
T6414 19135-19136 SYM denotes ′
T6415 19136-19137 HYPH denotes -
T6412 19137-19141 NN denotes tail
T6416 19142-19144 IN denotes on
T6417 19145-19148 NN denotes Mcm
T6418 19149-19157 NN denotes helicase
T6419 19158-19166 NN denotes activity
T6420 19166-19303 sentence denotes We next examined more systematically the effect of the compositions of the thymine residues of the 3′-tail on the Mcm helicase activity.
T6421 19167-19169 PRP denotes We
T6423 19170-19174 RB denotes next
T6422 19175-19183 VBD denotes examined
T6424 19184-19188 RBR denotes more
T6425 19189-19203 RB denotes systematically
T6426 19204-19207 DT denotes the
T6427 19208-19214 NN denotes effect
T6428 19215-19217 IN denotes of
T6429 19218-19221 DT denotes the
T6430 19222-19234 NNS denotes compositions
T6431 19235-19237 IN denotes of
T6432 19238-19241 DT denotes the
T6434 19242-19249 NN denotes thymine
T6433 19250-19258 NNS denotes residues
T6435 19259-19261 IN denotes of
T6436 19262-19265 DT denotes the
T6438 19266-19267 CD denotes 3
T6439 19267-19268 SYM denotes ′
T6440 19268-19269 HYPH denotes -
T6437 19269-19273 NN denotes tail
T6441 19274-19276 IN denotes on
T6442 19277-19280 DT denotes the
T6444 19281-19284 NN denotes Mcm
T6445 19285-19293 NN denotes helicase
T6443 19294-19302 NN denotes activity
T6446 19302-19303 . denotes .
T6447 19303-19550 sentence denotes A partial heteroduplex M13 single-stranded DNA containing a 37 bp duplex region in the presence or absence of the 50 nt long 3′-tail of various nucleotide compositions were constructed and the unwinding by mouse Mcm4/6/7 was examined (Figure 6A).
T6448 19304-19305 DT denotes A
T6450 19306-19313 JJ denotes partial
T6451 19314-19326 NN denotes heteroduplex
T6452 19327-19330 NN denotes M13
T6453 19331-19337 JJ denotes single
T6455 19337-19338 HYPH denotes -
T6454 19338-19346 VBN denotes stranded
T6449 19347-19350 NN denotes DNA
T6457 19351-19361 VBG denotes containing
T6458 19362-19363 DT denotes a
T6460 19364-19366 CD denotes 37
T6461 19367-19369 NN denotes bp
T6462 19370-19376 NN denotes duplex
T6459 19377-19383 NN denotes region
T6463 19384-19386 IN denotes in
T6464 19387-19390 DT denotes the
T6465 19391-19399 NN denotes presence
T6466 19400-19402 CC denotes or
T6467 19403-19410 NN denotes absence
T6468 19411-19413 IN denotes of
T6469 19414-19417 DT denotes the
T6471 19418-19420 CD denotes 50
T6472 19421-19423 NN denotes nt
T6473 19424-19428 JJ denotes long
T6474 19429-19430 CD denotes 3
T6475 19430-19431 SYM denotes ′
T6476 19431-19432 HYPH denotes -
T6470 19432-19436 NN denotes tail
T6477 19437-19439 IN denotes of
T6478 19440-19447 JJ denotes various
T6480 19448-19458 NN denotes nucleotide
T6479 19459-19471 NNS denotes compositions
T6481 19472-19476 VBD denotes were
T6456 19477-19488 VBN denotes constructed
T6482 19489-19492 CC denotes and
T6483 19493-19496 DT denotes the
T6484 19497-19506 NN denotes unwinding
T6486 19507-19509 IN denotes by
T6487 19510-19515 NN denotes mouse
T6488 19516-19520 NN denotes Mcm4
T6489 19520-19521 HYPH denotes /
T6490 19521-19522 CD denotes 6
T6491 19522-19523 HYPH denotes /
T6492 19523-19524 CD denotes 7
T6493 19525-19528 VBD denotes was
T6485 19529-19537 VBN denotes examined
T6494 19538-19539 -LRB- denotes (
T6496 19539-19545 NN denotes Figure
T6495 19546-19548 NN denotes 6A
T6497 19548-19549 -RRB- denotes )
T6498 19549-19550 . denotes .
T6499 19550-19678 sentence denotes Due to its low processivity, Mcm4/6/7 barely displaced the annealed 37mer oligonucleotide without a 3′-tail (37mer; Figure 6A).
T6500 19551-19554 IN denotes Due
T6502 19555-19557 IN denotes to
T6503 19558-19561 PRP$ denotes its
T6505 19562-19565 JJ denotes low
T6504 19566-19578 NN denotes processivity
T6506 19578-19580 , denotes ,
T6507 19580-19584 NN denotes Mcm4
T6508 19584-19585 HYPH denotes /
T6509 19585-19586 CD denotes 6
T6510 19586-19587 HYPH denotes /
T6511 19587-19588 CD denotes 7
T6512 19589-19595 RB denotes barely
T6501 19596-19605 VBD denotes displaced
T6513 19606-19609 DT denotes the
T6515 19610-19618 JJ denotes annealed
T6516 19619-19624 NN denotes 37mer
T6514 19625-19640 NN denotes oligonucleotide
T6517 19641-19648 IN denotes without
T6518 19649-19650 DT denotes a
T6520 19651-19652 CD denotes 3
T6521 19652-19653 SYM denotes ′
T6522 19653-19654 HYPH denotes -
T6519 19654-19658 NN denotes tail
T6523 19659-19660 -LRB- denotes (
T6525 19660-19665 NN denotes 37mer
T6526 19665-19666 : denotes ;
T6527 19667-19673 NN denotes Figure
T6524 19674-19676 NN denotes 6A
T6528 19676-19677 -RRB- denotes )
T6529 19677-19678 . denotes .
T6530 19678-19871 sentence denotes Only weak helicase activity was observed on the substrate carrying the 3′-tail of 25 repeats of TA dinucleotide (37-TA25; Figure 6A), whereas strong helicase activity was observed on 37-TTA17.
T6531 19679-19683 RB denotes Only
T6533 19684-19688 JJ denotes weak
T6534 19689-19697 NN denotes helicase
T6532 19698-19706 NN denotes activity
T6536 19707-19710 VBD denotes was
T6535 19711-19719 VBN denotes observed
T6537 19720-19722 IN denotes on
T6538 19723-19726 DT denotes the
T6539 19727-19736 NN denotes substrate
T6540 19737-19745 VBG denotes carrying
T6541 19746-19749 DT denotes the
T6543 19750-19751 CD denotes 3
T6544 19751-19752 SYM denotes ′
T6545 19752-19753 HYPH denotes -
T6542 19753-19757 NN denotes tail
T6546 19758-19760 IN denotes of
T6547 19761-19763 CD denotes 25
T6548 19764-19771 NNS denotes repeats
T6549 19772-19774 IN denotes of
T6550 19775-19777 NN denotes TA
T6551 19778-19790 NN denotes dinucleotide
T6552 19791-19792 -LRB- denotes (
T6554 19792-19794 CD denotes 37
T6556 19794-19795 HYPH denotes -
T6555 19795-19799 NN denotes TA25
T6557 19799-19800 : denotes ;
T6558 19801-19807 NN denotes Figure
T6553 19808-19810 NN denotes 6A
T6559 19810-19811 -RRB- denotes )
T6560 19811-19813 , denotes ,
T6561 19813-19820 IN denotes whereas
T6563 19821-19827 JJ denotes strong
T6565 19828-19836 NN denotes helicase
T6564 19837-19845 NN denotes activity
T6566 19846-19849 VBD denotes was
T6562 19850-19858 VBN denotes observed
T6567 19859-19861 IN denotes on
T6568 19862-19864 CD denotes 37
T6570 19864-19865 HYPH denotes -
T6569 19865-19870 NN denotes TTA17
T6571 19870-19871 . denotes .
T6572 19871-20121 sentence denotes Increase of thymine content on the 3′-tail did not further increase the extent of unwinding (37-TTTA13, 37-TTTTA10 and 37-T50; Figure 6A), suggesting that 17 repeats of TT dinucleotides (67% thymine) may be sufficient for activation of Mcm helicase.
T6573 19872-19880 NN denotes Increase
T6575 19881-19883 IN denotes of
T6576 19884-19891 NN denotes thymine
T6577 19892-19899 NN denotes content
T6578 19900-19902 IN denotes on
T6579 19903-19906 DT denotes the
T6581 19907-19908 CD denotes 3
T6582 19908-19909 SYM denotes ′
T6583 19909-19910 HYPH denotes -
T6580 19910-19914 NN denotes tail
T6584 19915-19918 VBD denotes did
T6585 19919-19922 RB denotes not
T6586 19923-19930 RB denotes further
T6574 19931-19939 VB denotes increase
T6587 19940-19943 DT denotes the
T6588 19944-19950 NN denotes extent
T6589 19951-19953 IN denotes of
T6590 19954-19963 NN denotes unwinding
T6591 19964-19965 -LRB- denotes (
T6593 19965-19967 CD denotes 37
T6595 19967-19968 HYPH denotes -
T6594 19968-19974 NN denotes TTTA13
T6596 19974-19976 , denotes ,
T6597 19976-19978 CD denotes 37
T6599 19978-19979 HYPH denotes -
T6598 19979-19986 NN denotes TTTTA10
T6600 19987-19990 CC denotes and
T6601 19991-19993 CD denotes 37
T6603 19993-19994 HYPH denotes -
T6602 19994-19997 NN denotes T50
T6604 19997-19998 : denotes ;
T6605 19999-20005 NN denotes Figure
T6592 20006-20008 NN denotes 6A
T6606 20008-20009 -RRB- denotes )
T6607 20009-20011 , denotes ,
T6608 20011-20021 VBG denotes suggesting
T6609 20022-20026 IN denotes that
T6611 20027-20029 CD denotes 17
T6612 20030-20037 NNS denotes repeats
T6613 20038-20040 IN denotes of
T6614 20041-20043 NN denotes TT
T6615 20044-20057 NNS denotes dinucleotides
T6616 20058-20059 -LRB- denotes (
T6618 20059-20061 CD denotes 67
T6619 20061-20062 NN denotes %
T6617 20063-20070 NN denotes thymine
T6620 20070-20071 -RRB- denotes )
T6621 20072-20075 MD denotes may
T6610 20076-20078 VB denotes be
T6622 20079-20089 JJ denotes sufficient
T6623 20090-20093 IN denotes for
T6624 20094-20104 NN denotes activation
T6625 20105-20107 IN denotes of
T6626 20108-20111 NN denotes Mcm
T6627 20112-20120 NN denotes helicase
T6628 20120-20121 . denotes .
T6629 20121-20322 sentence denotes It should be noted that the TA25 tail would self-anneal and this would prevent the loading of Mcm, since the 3′-tail containing TTAA13 or TTTAAA8 did not enhance the unwinding either (data not shown).
T6630 20122-20124 PRP denotes It
T6632 20125-20131 MD denotes should
T6633 20132-20134 VB denotes be
T6631 20135-20140 VBN denotes noted
T6634 20141-20145 IN denotes that
T6636 20146-20149 DT denotes the
T6638 20150-20154 NN denotes TA25
T6637 20155-20159 NN denotes tail
T6639 20160-20165 MD denotes would
T6640 20166-20170 NN denotes self
T6641 20170-20171 HYPH denotes -
T6635 20171-20177 VB denotes anneal
T6642 20178-20181 CC denotes and
T6643 20182-20186 DT denotes this
T6645 20187-20192 MD denotes would
T6644 20193-20200 VB denotes prevent
T6646 20201-20204 DT denotes the
T6647 20205-20212 NN denotes loading
T6648 20213-20215 IN denotes of
T6649 20216-20219 NN denotes Mcm
T6650 20219-20221 , denotes ,
T6651 20221-20226 IN denotes since
T6653 20227-20230 DT denotes the
T6655 20231-20232 CD denotes 3
T6656 20232-20233 SYM denotes ′
T6657 20233-20234 HYPH denotes -
T6654 20234-20238 NN denotes tail
T6658 20239-20249 VBG denotes containing
T6659 20250-20256 NN denotes TTAA13
T6660 20257-20259 CC denotes or
T6661 20260-20267 NN denotes TTTAAA8
T6662 20268-20271 VBD denotes did
T6663 20272-20275 RB denotes not
T6652 20276-20283 VB denotes enhance
T6664 20284-20287 DT denotes the
T6665 20288-20297 NN denotes unwinding
T6666 20298-20304 RB denotes either
T6667 20305-20306 -LRB- denotes (
T6669 20306-20310 NNS denotes data
T6670 20311-20314 RB denotes not
T6668 20315-20320 VBN denotes shown
T6671 20320-20321 -RRB- denotes )
T6672 20321-20322 . denotes .
T6673 20322-20422 sentence denotes Therefore, we next examined the effects of repeating sequences of thymine and cytosine (Figure 6B).
T6674 20323-20332 RB denotes Therefore
T6676 20332-20334 , denotes ,
T6677 20334-20336 PRP denotes we
T6678 20337-20341 RB denotes next
T6675 20342-20350 VBD denotes examined
T6679 20351-20354 DT denotes the
T6680 20355-20362 NNS denotes effects
T6681 20363-20365 IN denotes of
T6682 20366-20375 VBG denotes repeating
T6683 20376-20385 NNS denotes sequences
T6684 20386-20388 IN denotes of
T6685 20389-20396 NN denotes thymine
T6686 20397-20400 CC denotes and
T6687 20401-20409 NN denotes cytosine
T6688 20410-20411 -LRB- denotes (
T6690 20411-20417 NN denotes Figure
T6689 20418-20420 NN denotes 6B
T6691 20420-20421 -RRB- denotes )
T6692 20421-20422 . denotes .
T6693 20422-20696 sentence denotes Partial heteroduplex substrates containing the 3′-tail of TC25, TTCC13, TTTCCC8, TTTTCCCC6, TTCC17 were displaced by the Mcm4/6/7 complex to the extent similar to that achieved by TTA17, suggesting that 50% thymine content is sufficient for full activation of Mcm helicase.
T6694 20423-20430 JJ denotes Partial
T6696 20431-20443 NN denotes heteroduplex
T6695 20444-20454 NNS denotes substrates
T6698 20455-20465 VBG denotes containing
T6699 20466-20469 DT denotes the
T6701 20470-20471 CD denotes 3
T6702 20471-20472 SYM denotes ′
T6703 20472-20473 HYPH denotes -
T6700 20473-20477 NN denotes tail
T6704 20478-20480 IN denotes of
T6705 20481-20485 NN denotes TC25
T6707 20485-20487 , denotes ,
T6708 20487-20493 NN denotes TTCC13
T6709 20493-20495 , denotes ,
T6710 20495-20502 NN denotes TTTCCC8
T6711 20502-20504 , denotes ,
T6712 20504-20513 NN denotes TTTTCCCC6
T6713 20513-20515 , denotes ,
T6706 20515-20521 NN denotes TTCC17
T6714 20522-20526 VBD denotes were
T6697 20527-20536 VBN denotes displaced
T6715 20537-20539 IN denotes by
T6716 20540-20543 DT denotes the
T6718 20544-20548 NN denotes Mcm4
T6719 20548-20549 HYPH denotes /
T6720 20549-20550 CD denotes 6
T6721 20550-20551 HYPH denotes /
T6722 20551-20552 CD denotes 7
T6717 20553-20560 NN denotes complex
T6723 20561-20563 IN denotes to
T6724 20564-20567 DT denotes the
T6725 20568-20574 NN denotes extent
T6726 20575-20582 JJ denotes similar
T6727 20583-20585 IN denotes to
T6728 20586-20590 DT denotes that
T6729 20591-20599 VBN denotes achieved
T6730 20600-20602 IN denotes by
T6731 20603-20608 NN denotes TTA17
T6732 20608-20610 , denotes ,
T6733 20610-20620 VBG denotes suggesting
T6734 20621-20625 IN denotes that
T6736 20626-20628 CD denotes 50
T6737 20628-20629 NN denotes %
T6739 20630-20637 NN denotes thymine
T6738 20638-20645 NN denotes content
T6735 20646-20648 VBZ denotes is
T6740 20649-20659 JJ denotes sufficient
T6741 20660-20663 IN denotes for
T6742 20664-20668 JJ denotes full
T6743 20669-20679 NN denotes activation
T6744 20680-20682 IN denotes of
T6745 20683-20686 NN denotes Mcm
T6746 20687-20695 NN denotes helicase
T6747 20695-20696 . denotes .
T6748 20696-20838 sentence denotes TTCC13 and TTCC17 were displaced to a similar extent, consistent with the earlier result that a 40 nt tail is sufficient for activation (13).
T6749 20697-20703 NN denotes TTCC13
T6751 20704-20707 CC denotes and
T6752 20708-20714 NN denotes TTCC17
T6753 20715-20719 VBD denotes were
T6750 20720-20729 VBN denotes displaced
T6754 20730-20732 IN denotes to
T6755 20733-20734 DT denotes a
T6757 20735-20742 JJ denotes similar
T6756 20743-20749 NN denotes extent
T6758 20749-20751 , denotes ,
T6759 20751-20761 JJ denotes consistent
T6760 20762-20766 IN denotes with
T6761 20767-20770 DT denotes the
T6763 20771-20778 JJR denotes earlier
T6762 20779-20785 NN denotes result
T6764 20786-20790 IN denotes that
T6766 20791-20792 DT denotes a
T6768 20793-20795 CD denotes 40
T6769 20796-20798 NN denotes nt
T6767 20799-20803 NN denotes tail
T6765 20804-20806 VBZ denotes is
T6770 20807-20817 JJ denotes sufficient
T6771 20818-20821 IN denotes for
T6772 20822-20832 NN denotes activation
T6773 20833-20834 -LRB- denotes (
T6774 20834-20836 CD denotes 13
T6775 20836-20837 -RRB- denotes )
T6776 20837-20838 . denotes .
T6777 20838-21000 sentence denotes However, the efficiency of the displacement significantly decreased as the thymine content of the 3′-tail dropped to 33%, as in TAA17 or TCC17 (Figure 6B and C).
T6778 20839-20846 RB denotes However
T6780 20846-20848 , denotes ,
T6781 20848-20851 DT denotes the
T6782 20852-20862 NN denotes efficiency
T6783 20863-20865 IN denotes of
T6784 20866-20869 DT denotes the
T6785 20870-20882 NN denotes displacement
T6786 20883-20896 RB denotes significantly
T6779 20897-20906 VBD denotes decreased
T6787 20907-20909 IN denotes as
T6789 20910-20913 DT denotes the
T6791 20914-20921 NN denotes thymine
T6790 20922-20929 NN denotes content
T6792 20930-20932 IN denotes of
T6793 20933-20936 DT denotes the
T6795 20937-20938 CD denotes 3
T6796 20938-20939 SYM denotes ′
T6797 20939-20940 HYPH denotes -
T6794 20940-20944 NN denotes tail
T6788 20945-20952 VBD denotes dropped
T6798 20953-20955 IN denotes to
T6799 20956-20958 CD denotes 33
T6800 20958-20959 NN denotes %
T6801 20959-20961 , denotes ,
T6802 20961-20963 IN denotes as
T6803 20964-20966 IN denotes in
T6804 20967-20972 NN denotes TAA17
T6805 20973-20975 CC denotes or
T6806 20976-20981 NN denotes TCC17
T6807 20982-20983 -LRB- denotes (
T6809 20983-20989 NN denotes Figure
T6808 20990-20992 NN denotes 6B
T6810 20993-20996 CC denotes and
T6811 20997-20998 NN denotes C
T6812 20998-20999 -RRB- denotes )
T6813 20999-21000 . denotes .
T6814 21000-21115 sentence denotes Thus, the minimal thymine content required for activation of the Mcm helicase may be somewhere between 33 and 50%.
T6815 21001-21005 RB denotes Thus
T6817 21005-21007 , denotes ,
T6818 21007-21010 DT denotes the
T6820 21011-21018 JJ denotes minimal
T6821 21019-21026 NN denotes thymine
T6819 21027-21034 NN denotes content
T6822 21035-21043 VBN denotes required
T6823 21044-21047 IN denotes for
T6824 21048-21058 NN denotes activation
T6825 21059-21061 IN denotes of
T6826 21062-21065 DT denotes the
T6828 21066-21069 NN denotes Mcm
T6827 21070-21078 NN denotes helicase
T6829 21079-21082 MD denotes may
T6816 21083-21085 VB denotes be
T6830 21086-21095 RB denotes somewhere
T6831 21096-21103 IN denotes between
T6832 21104-21106 CD denotes 33
T6834 21107-21110 CC denotes and
T6835 21111-21113 CD denotes 50
T6833 21113-21114 NN denotes %
T6836 21114-21115 . denotes .
T7008 21117-21120 NN denotes DUE
T7009 21121-21130 NNS denotes sequences
T7011 21131-21135 IN denotes from
T7012 21136-21139 DT denotes the
T7014 21140-21141 NN denotes c
T7016 21141-21142 HYPH denotes -
T7015 21142-21145 NN denotes myc
T7013 21146-21152 NN denotes origin
T7010 21153-21161 VBP denotes activate
T7017 21162-21165 NN denotes Mcm
T7018 21166-21174 NN denotes helicase
T7019 21174-21474 sentence denotes Our previous studies have shown that the sequences containing periodic (dT)n tracts derived from the human lamin B2 origin (48% thymine content) can serve as an efficient activator for the Mcm helicase on a bubble, and replacement of thymines with guanines abolished the helicase activation (13,28).
T7020 21175-21178 PRP$ denotes Our
T7022 21179-21187 JJ denotes previous
T7021 21188-21195 NNS denotes studies
T7024 21196-21200 VBP denotes have
T7023 21201-21206 VBN denotes shown
T7025 21207-21211 IN denotes that
T7027 21212-21215 DT denotes the
T7028 21216-21225 NNS denotes sequences
T7029 21226-21236 VBG denotes containing
T7030 21237-21245 JJ denotes periodic
T7032 21246-21247 -LRB- denotes (
T7034 21247-21249 NN denotes dT
T7035 21249-21250 -RRB- denotes )
T7033 21250-21251 NN denotes n
T7031 21252-21258 NNS denotes tracts
T7036 21259-21266 VBN denotes derived
T7037 21267-21271 IN denotes from
T7038 21272-21275 DT denotes the
T7040 21276-21281 JJ denotes human
T7041 21282-21287 NN denotes lamin
T7042 21288-21290 NN denotes B2
T7039 21291-21297 NN denotes origin
T7043 21298-21299 -LRB- denotes (
T7045 21299-21301 CD denotes 48
T7046 21301-21302 NN denotes %
T7047 21303-21310 NN denotes thymine
T7044 21311-21318 NN denotes content
T7048 21318-21319 -RRB- denotes )
T7049 21320-21323 MD denotes can
T7026 21324-21329 VB denotes serve
T7050 21330-21332 IN denotes as
T7051 21333-21335 DT denotes an
T7053 21336-21345 JJ denotes efficient
T7052 21346-21355 NN denotes activator
T7054 21356-21359 IN denotes for
T7055 21360-21363 DT denotes the
T7057 21364-21367 NN denotes Mcm
T7056 21368-21376 NN denotes helicase
T7058 21377-21379 IN denotes on
T7059 21380-21381 DT denotes a
T7060 21382-21388 NN denotes bubble
T7061 21388-21390 , denotes ,
T7062 21390-21393 CC denotes and
T7063 21394-21405 NN denotes replacement
T7065 21406-21408 IN denotes of
T7066 21409-21417 NNS denotes thymines
T7067 21418-21422 IN denotes with
T7068 21423-21431 NNS denotes guanines
T7064 21432-21441 VBD denotes abolished
T7069 21442-21445 DT denotes the
T7071 21446-21454 NN denotes helicase
T7070 21455-21465 NN denotes activation
T7072 21466-21467 -LRB- denotes (
T7074 21467-21469 CD denotes 13
T7075 21469-21470 , denotes ,
T7073 21470-21472 CD denotes 28
T7076 21472-21473 -RRB- denotes )
T7077 21473-21474 . denotes .
T7078 21474-21832 sentence denotes In order to examine other natural replication origins with different thymine contents for the ability to activate Mcm helicase on a bubble substrate, we constructed new bubble substrates, Bub82/c-myc, containing sequences derived from the DUE (DNA unwinding element) region of the human c-myc origin which is essential for c-myc replicator activity (29,30).
T7079 21475-21477 IN denotes In
T7081 21478-21483 NN denotes order
T7082 21484-21486 TO denotes to
T7083 21487-21494 VB denotes examine
T7084 21495-21500 JJ denotes other
T7086 21501-21508 JJ denotes natural
T7087 21509-21520 NN denotes replication
T7085 21521-21528 NNS denotes origins
T7088 21529-21533 IN denotes with
T7089 21534-21543 JJ denotes different
T7091 21544-21551 NN denotes thymine
T7090 21552-21560 NNS denotes contents
T7092 21561-21564 IN denotes for
T7093 21565-21568 DT denotes the
T7094 21569-21576 NN denotes ability
T7095 21577-21579 TO denotes to
T7096 21580-21588 VB denotes activate
T7097 21589-21592 NN denotes Mcm
T7098 21593-21601 NN denotes helicase
T7099 21602-21604 IN denotes on
T7100 21605-21606 DT denotes a
T7102 21607-21613 NN denotes bubble
T7101 21614-21623 NN denotes substrate
T7103 21623-21625 , denotes ,
T7104 21625-21627 PRP denotes we
T7080 21628-21639 VBD denotes constructed
T7105 21640-21643 JJ denotes new
T7107 21644-21650 NN denotes bubble
T7106 21651-21661 NNS denotes substrates
T7108 21661-21663 , denotes ,
T7109 21663-21668 NN denotes Bub82
T7111 21668-21669 HYPH denotes /
T7112 21669-21670 NN denotes c
T7113 21670-21671 HYPH denotes -
T7110 21671-21674 NN denotes myc
T7114 21674-21676 , denotes ,
T7115 21676-21686 VBG denotes containing
T7116 21687-21696 NNS denotes sequences
T7117 21697-21704 VBN denotes derived
T7118 21705-21709 IN denotes from
T7119 21710-21713 DT denotes the
T7121 21714-21717 NN denotes DUE
T7122 21718-21719 -LRB- denotes (
T7123 21719-21722 NN denotes DNA
T7125 21723-21732 NN denotes unwinding
T7124 21733-21740 NN denotes element
T7126 21740-21741 -RRB- denotes )
T7120 21742-21748 NN denotes region
T7127 21749-21751 IN denotes of
T7128 21752-21755 DT denotes the
T7130 21756-21761 JJ denotes human
T7131 21762-21763 NN denotes c
T7133 21763-21764 HYPH denotes -
T7132 21764-21767 NN denotes myc
T7129 21768-21774 NN denotes origin
T7134 21775-21780 WDT denotes which
T7135 21781-21783 VBZ denotes is
T7136 21784-21793 JJ denotes essential
T7137 21794-21797 IN denotes for
T7138 21798-21799 NN denotes c
T7140 21799-21800 HYPH denotes -
T7139 21800-21803 NN denotes myc
T7142 21804-21814 NN denotes replicator
T7141 21815-21823 NN denotes activity
T7143 21824-21825 -LRB- denotes (
T7145 21825-21827 CD denotes 29
T7146 21827-21828 , denotes ,
T7144 21828-21830 CD denotes 30
T7147 21830-21831 -RRB- denotes )
T7148 21831-21832 . denotes .
T7149 21832-21954 sentence denotes The unpaired segment in Bub82/c-myc/DUE contains one strand of DUE and that in Bub82/c-myc/DUE-C contains another strand.
T7150 21833-21836 DT denotes The
T7152 21837-21845 JJ denotes unpaired
T7151 21846-21853 NN denotes segment
T7154 21854-21856 IN denotes in
T7155 21857-21862 NN denotes Bub82
T7157 21862-21863 HYPH denotes /
T7158 21863-21864 NN denotes c
T7160 21864-21865 HYPH denotes -
T7159 21865-21868 NN denotes myc
T7161 21868-21869 HYPH denotes /
T7156 21869-21872 NN denotes DUE
T7153 21873-21881 VBZ denotes contains
T7162 21882-21885 CD denotes one
T7163 21886-21892 NN denotes strand
T7164 21893-21895 IN denotes of
T7165 21896-21899 NN denotes DUE
T7166 21900-21903 CC denotes and
T7167 21904-21908 DT denotes that
T7169 21909-21911 IN denotes in
T7170 21912-21917 NN denotes Bub82
T7172 21917-21918 HYPH denotes /
T7173 21918-21919 NN denotes c
T7175 21919-21920 HYPH denotes -
T7174 21920-21923 NN denotes myc
T7176 21923-21924 HYPH denotes /
T7177 21924-21927 NN denotes DUE
T7178 21927-21928 HYPH denotes -
T7171 21928-21929 NN denotes C
T7168 21930-21938 VBZ denotes contains
T7179 21939-21946 DT denotes another
T7180 21947-21953 NN denotes strand
T7181 21953-21954 . denotes .
T7182 21954-22116 sentence denotes This DUE is believed to coincide with the initially unwound region of the c-myc origin (29), and its deletion substantially reduced the replicator activity (30).
T7183 21955-21959 DT denotes This
T7184 21960-21963 NN denotes DUE
T7186 21964-21966 VBZ denotes is
T7185 21967-21975 VBN denotes believed
T7187 21976-21978 TO denotes to
T7188 21979-21987 VB denotes coincide
T7189 21988-21992 IN denotes with
T7190 21993-21996 DT denotes the
T7192 21997-22006 RB denotes initially
T7193 22007-22014 JJ denotes unwound
T7191 22015-22021 NN denotes region
T7194 22022-22024 IN denotes of
T7195 22025-22028 DT denotes the
T7197 22029-22030 NN denotes c
T7199 22030-22031 HYPH denotes -
T7198 22031-22034 NN denotes myc
T7196 22035-22041 NN denotes origin
T7200 22042-22043 -LRB- denotes (
T7201 22043-22045 CD denotes 29
T7202 22045-22046 -RRB- denotes )
T7203 22046-22048 , denotes ,
T7204 22048-22051 CC denotes and
T7205 22052-22055 PRP$ denotes its
T7206 22056-22064 NN denotes deletion
T7208 22065-22078 RB denotes substantially
T7207 22079-22086 VBD denotes reduced
T7209 22087-22090 DT denotes the
T7211 22091-22101 NN denotes replicator
T7210 22102-22110 NN denotes activity
T7212 22111-22112 -LRB- denotes (
T7213 22112-22114 CD denotes 30
T7214 22114-22115 -RRB- denotes )
T7215 22115-22116 . denotes .
T7216 22116-22362 sentence denotes Mcm4/6/7 unwound Bub82/lamin B2 (thymine 48%), Bub82/c-myc/DUE (37%) and Bub82/c-myc/DUE-C (39%) with similar efficiency (Figure 7A), indicating that the sequences from the c-myc origin also can serve as efficient activators of the Mcm helicase.
T7217 22117-22121 NN denotes Mcm4
T7219 22121-22122 HYPH denotes /
T7220 22122-22123 CD denotes 6
T7221 22123-22124 HYPH denotes /
T7222 22124-22125 CD denotes 7
T7218 22126-22133 VBN denotes unwound
T7223 22134-22139 NN denotes Bub82
T7225 22139-22140 HYPH denotes /
T7226 22140-22145 NN denotes lamin
T7224 22146-22148 NN denotes B2
T7227 22149-22150 -LRB- denotes (
T7229 22150-22157 NN denotes thymine
T7230 22158-22160 CD denotes 48
T7228 22160-22161 NN denotes %
T7231 22161-22162 -RRB- denotes )
T7232 22162-22164 , denotes ,
T7233 22164-22169 NN denotes Bub82
T7235 22169-22170 HYPH denotes /
T7236 22170-22171 NN denotes c
T7238 22171-22172 HYPH denotes -
T7237 22172-22175 NN denotes myc
T7239 22175-22176 HYPH denotes /
T7234 22176-22179 NN denotes DUE
T7240 22180-22181 -LRB- denotes (
T7242 22181-22183 CD denotes 37
T7241 22183-22184 NN denotes %
T7243 22184-22185 -RRB- denotes )
T7244 22186-22189 CC denotes and
T7245 22190-22195 NN denotes Bub82
T7247 22195-22196 HYPH denotes /
T7248 22196-22197 NN denotes c
T7250 22197-22198 HYPH denotes -
T7249 22198-22201 NN denotes myc
T7251 22201-22202 HYPH denotes /
T7252 22202-22205 NN denotes DUE
T7253 22205-22206 HYPH denotes -
T7246 22206-22207 NN denotes C
T7254 22208-22209 -LRB- denotes (
T7256 22209-22211 CD denotes 39
T7255 22211-22212 NN denotes %
T7257 22212-22213 -RRB- denotes )
T7258 22214-22218 IN denotes with
T7259 22219-22226 JJ denotes similar
T7260 22227-22237 NN denotes efficiency
T7261 22238-22239 -LRB- denotes (
T7263 22239-22245 NN denotes Figure
T7262 22246-22248 NN denotes 7A
T7264 22248-22249 -RRB- denotes )
T7265 22249-22251 , denotes ,
T7266 22251-22261 VBG denotes indicating
T7267 22262-22266 IN denotes that
T7269 22267-22270 DT denotes the
T7270 22271-22280 NNS denotes sequences
T7271 22281-22285 IN denotes from
T7272 22286-22289 DT denotes the
T7274 22290-22291 NN denotes c
T7276 22291-22292 HYPH denotes -
T7275 22292-22295 NN denotes myc
T7273 22296-22302 NN denotes origin
T7277 22303-22307 RB denotes also
T7278 22308-22311 MD denotes can
T7268 22312-22317 VB denotes serve
T7279 22318-22320 IN denotes as
T7280 22321-22330 JJ denotes efficient
T7281 22331-22341 NNS denotes activators
T7282 22342-22344 IN denotes of
T7283 22345-22348 DT denotes the
T7285 22349-22352 NN denotes Mcm
T7284 22353-22361 NN denotes helicase
T7286 22361-22362 . denotes .
T7287 22362-22628 sentence denotes This result also indicates that sequences with 37% thymine content can activate Mcm4/6/7 to displace 24 nt duplex on both sides in a bubble substrate, suggesting that the not only thymine content but also the sequence context may affect the Mcm helicase activation.
T7288 22363-22367 DT denotes This
T7289 22368-22374 NN denotes result
T7291 22375-22379 RB denotes also
T7290 22380-22389 VBZ denotes indicates
T7292 22390-22394 IN denotes that
T7294 22395-22404 NNS denotes sequences
T7295 22405-22409 IN denotes with
T7296 22410-22412 CD denotes 37
T7297 22412-22413 NN denotes %
T7299 22414-22421 NN denotes thymine
T7298 22422-22429 NN denotes content
T7300 22430-22433 MD denotes can
T7293 22434-22442 VB denotes activate
T7301 22443-22447 NN denotes Mcm4
T7302 22447-22448 HYPH denotes /
T7303 22448-22449 CD denotes 6
T7304 22449-22450 HYPH denotes /
T7305 22450-22451 CD denotes 7
T7306 22452-22454 TO denotes to
T7307 22455-22463 VB denotes displace
T7308 22464-22466 CD denotes 24
T7309 22467-22469 NN denotes nt
T7310 22470-22476 NN denotes duplex
T7311 22477-22479 IN denotes on
T7312 22480-22484 DT denotes both
T7313 22485-22490 NNS denotes sides
T7314 22491-22493 IN denotes in
T7315 22494-22495 DT denotes a
T7317 22496-22502 NN denotes bubble
T7316 22503-22512 NN denotes substrate
T7318 22512-22514 , denotes ,
T7319 22514-22524 VBG denotes suggesting
T7320 22525-22529 IN denotes that
T7322 22530-22533 DT denotes the
T7324 22534-22537 RB denotes not
T7325 22538-22542 RB denotes only
T7326 22543-22550 NN denotes thymine
T7323 22551-22558 NN denotes content
T7327 22559-22562 CC denotes but
T7328 22563-22567 RB denotes also
T7329 22568-22571 DT denotes the
T7331 22572-22580 NN denotes sequence
T7330 22581-22588 NN denotes context
T7332 22589-22592 MD denotes may
T7321 22593-22599 VB denotes affect
T7333 22600-22603 DT denotes the
T7335 22604-22607 NN denotes Mcm
T7336 22608-22616 NN denotes helicase
T7334 22617-22627 NN denotes activation
T7337 22627-22628 . denotes .
T7338 22628-22816 sentence denotes In gel shift assays, MCM4/6/7 bound to all the three bubbles, although the affinity to Bub82/c-myc/DUE and to Bub82/c-myc/DUE-C is slightly lower than that to Bub-82/lamin B2 (Figure 7B).
T7339 22629-22631 IN denotes In
T7341 22632-22635 NN denotes gel
T7342 22636-22641 NN denotes shift
T7343 22642-22648 NNS denotes assays
T7344 22648-22650 , denotes ,
T7345 22650-22654 NN denotes MCM4
T7346 22654-22655 HYPH denotes /
T7347 22655-22656 CD denotes 6
T7348 22656-22657 HYPH denotes /
T7349 22657-22658 CD denotes 7
T7340 22659-22664 VBD denotes bound
T7350 22665-22667 IN denotes to
T7351 22668-22671 PDT denotes all
T7353 22672-22675 DT denotes the
T7354 22676-22681 CD denotes three
T7352 22682-22689 NNS denotes bubbles
T7355 22689-22691 , denotes ,
T7356 22691-22699 IN denotes although
T7358 22700-22703 DT denotes the
T7359 22704-22712 NN denotes affinity
T7360 22713-22715 IN denotes to
T7361 22716-22721 NN denotes Bub82
T7363 22721-22722 HYPH denotes /
T7364 22722-22723 NN denotes c
T7366 22723-22724 HYPH denotes -
T7365 22724-22727 NN denotes myc
T7367 22727-22728 HYPH denotes /
T7362 22728-22731 NN denotes DUE
T7368 22732-22735 CC denotes and
T7369 22736-22738 IN denotes to
T7370 22739-22744 NN denotes Bub82
T7372 22744-22745 HYPH denotes /
T7373 22745-22746 NN denotes c
T7375 22746-22747 HYPH denotes -
T7374 22747-22750 NN denotes myc
T7376 22750-22751 HYPH denotes /
T7377 22751-22754 NN denotes DUE
T7378 22754-22755 HYPH denotes -
T7371 22755-22756 NN denotes C
T7357 22757-22759 VBZ denotes is
T7379 22760-22768 RB denotes slightly
T7380 22769-22774 JJR denotes lower
T7381 22775-22779 IN denotes than
T7382 22780-22784 DT denotes that
T7383 22785-22787 IN denotes to
T7384 22788-22791 NN denotes Bub
T7386 22791-22792 HYPH denotes -
T7387 22792-22794 CD denotes 82
T7388 22794-22795 HYPH denotes /
T7389 22795-22800 NN denotes lamin
T7385 22801-22803 NN denotes B2
T7390 22804-22805 -LRB- denotes (
T7392 22805-22811 NN denotes Figure
T7391 22812-22814 NN denotes 7B
T7393 22814-22815 -RRB- denotes )
T7394 22815-22816 . denotes .
T7395 22816-22909 sentence denotes Therefore, helicase and DNA binding activities do not necessarily correlate with each other.
T7396 22817-22826 RB denotes Therefore
T7398 22826-22828 , denotes ,
T7399 22828-22836 NN denotes helicase
T7401 22837-22840 CC denotes and
T7402 22841-22844 NN denotes DNA
T7403 22845-22852 NN denotes binding
T7400 22853-22863 NNS denotes activities
T7404 22864-22866 VBP denotes do
T7405 22867-22870 RB denotes not
T7406 22871-22882 RB denotes necessarily
T7397 22883-22892 VB denotes correlate
T7407 22893-22897 IN denotes with
T7408 22898-22902 DT denotes each
T7409 22903-22908 JJ denotes other
T7410 22908-22909 . denotes .
T7411 22909-23048 sentence denotes This was also indicated by our previous results that guanine and cytosine stretches can bind to Mcm but cannot activate the helicase (13).
T7412 22910-22914 DT denotes This
T7414 22915-22918 VBD denotes was
T7415 22919-22923 RB denotes also
T7413 22924-22933 VBN denotes indicated
T7416 22934-22936 IN denotes by
T7417 22937-22940 PRP$ denotes our
T7419 22941-22949 JJ denotes previous
T7418 22950-22957 NNS denotes results
T7420 22958-22962 IN denotes that
T7422 22963-22970 NN denotes guanine
T7424 22971-22974 CC denotes and
T7425 22975-22983 NN denotes cytosine
T7423 22984-22993 NNS denotes stretches
T7426 22994-22997 MD denotes can
T7421 22998-23002 VB denotes bind
T7427 23003-23005 IN denotes to
T7428 23006-23009 NN denotes Mcm
T7429 23010-23013 CC denotes but
T7430 23014-23017 MD denotes can
T7432 23017-23020 RB denotes not
T7431 23021-23029 VB denotes activate
T7433 23030-23033 DT denotes the
T7434 23034-23042 NN denotes helicase
T7435 23043-23044 -LRB- denotes (
T7436 23044-23046 CD denotes 13
T7437 23046-23047 -RRB- denotes )
T7438 23047-23048 . denotes .
T7722 23050-23053 NN denotes DNA
T7723 23054-23063 NN denotes unwinding
T7725 23064-23066 IN denotes by
T7726 23067-23071 NN denotes Mcm4
T7727 23071-23072 HYPH denotes /
T7728 23072-23073 CD denotes 6
T7729 23073-23074 HYPH denotes /
T7730 23074-23075 CD denotes 7
T7731 23076-23078 VBZ denotes is
T7724 23079-23088 VBN denotes inhibited
T7732 23089-23091 IN denotes by
T7733 23092-23095 DT denotes the
T7735 23096-23098 NN denotes GC
T7737 23098-23099 HYPH denotes -
T7736 23099-23103 JJ denotes rich
T7734 23104-23113 NNS denotes sequences
T7738 23114-23116 IN denotes on
T7739 23117-23120 DT denotes the
T7741 23121-23127 NN denotes duplex
T7740 23128-23135 NN denotes segment
T7742 23135-23262 sentence denotes Our results indicate that thymine-rich single-stranded DNA is required for initial loading and activation of the Mcm helicase.
T7743 23136-23139 PRP$ denotes Our
T7744 23140-23147 NNS denotes results
T7745 23148-23156 VBP denotes indicate
T7746 23157-23161 IN denotes that
T7748 23162-23169 NN denotes thymine
T7750 23169-23170 HYPH denotes -
T7749 23170-23174 JJ denotes rich
T7752 23175-23181 JJ denotes single
T7754 23181-23182 HYPH denotes -
T7753 23182-23190 VBN denotes stranded
T7751 23191-23194 NN denotes DNA
T7755 23195-23197 VBZ denotes is
T7747 23198-23206 VBN denotes required
T7756 23207-23210 IN denotes for
T7757 23211-23218 JJ denotes initial
T7758 23219-23226 NN denotes loading
T7759 23227-23230 CC denotes and
T7760 23231-23241 NN denotes activation
T7761 23242-23244 IN denotes of
T7762 23245-23248 DT denotes the
T7764 23249-23252 NN denotes Mcm
T7763 23253-23261 NN denotes helicase
T7765 23261-23262 . denotes .
T7766 23262-23366 sentence denotes However, it is not known whether thymine sequences are required for processive unwinding of duplex DNA.
T7767 23263-23270 RB denotes However
T7769 23270-23272 , denotes ,
T7770 23272-23274 PRP denotes it
T7771 23275-23277 VBZ denotes is
T7772 23278-23281 RB denotes not
T7768 23282-23287 VBN denotes known
T7773 23288-23295 IN denotes whether
T7775 23296-23303 NN denotes thymine
T7776 23304-23313 NNS denotes sequences
T7777 23314-23317 VBP denotes are
T7774 23318-23326 VBN denotes required
T7778 23327-23330 IN denotes for
T7779 23331-23341 JJ denotes processive
T7780 23342-23351 NN denotes unwinding
T7781 23352-23354 IN denotes of
T7782 23355-23361 NN denotes duplex
T7783 23362-23365 NN denotes DNA
T7784 23365-23366 . denotes .
T7785 23366-23482 sentence denotes Therefore, we have examined whether the nucleotide composition of the duplex region affects its unwinding activity.
T7786 23367-23376 RB denotes Therefore
T7788 23376-23378 , denotes ,
T7789 23378-23380 PRP denotes we
T7790 23381-23385 VBP denotes have
T7787 23386-23394 VBN denotes examined
T7791 23395-23402 IN denotes whether
T7793 23403-23406 DT denotes the
T7795 23407-23417 NN denotes nucleotide
T7794 23418-23429 NN denotes composition
T7796 23430-23432 IN denotes of
T7797 23433-23436 DT denotes the
T7799 23437-23443 NN denotes duplex
T7798 23444-23450 NN denotes region
T7792 23451-23458 VBZ denotes affects
T7800 23459-23462 PRP$ denotes its
T7802 23463-23472 NN denotes unwinding
T7801 23473-23481 NN denotes activity
T7803 23481-23482 . denotes .
T7804 23482-23620 sentence denotes To address this issue, we constructed a series of T-tailed Y-fork structures (T-fork) containing various sequences in the duplex segment.
T7805 23483-23485 TO denotes To
T7806 23486-23493 VB denotes address
T7808 23494-23498 DT denotes this
T7809 23499-23504 NN denotes issue
T7810 23504-23506 , denotes ,
T7811 23506-23508 PRP denotes we
T7807 23509-23520 VBD denotes constructed
T7812 23521-23522 DT denotes a
T7813 23523-23529 NN denotes series
T7814 23530-23532 IN denotes of
T7815 23533-23534 NN denotes T
T7817 23534-23535 HYPH denotes -
T7816 23535-23541 VBN denotes tailed
T7819 23542-23543 NN denotes Y
T7821 23543-23544 HYPH denotes -
T7820 23544-23548 NN denotes fork
T7818 23549-23559 NNS denotes structures
T7822 23560-23561 -LRB- denotes (
T7823 23561-23562 NN denotes T
T7825 23562-23563 HYPH denotes -
T7824 23563-23567 NN denotes fork
T7826 23567-23568 -RRB- denotes )
T7827 23569-23579 VBG denotes containing
T7828 23580-23587 JJ denotes various
T7829 23588-23597 NNS denotes sequences
T7830 23598-23600 IN denotes in
T7831 23601-23604 DT denotes the
T7833 23605-23611 NN denotes duplex
T7832 23612-23619 NN denotes segment
T7834 23619-23620 . denotes .
T7835 23620-23693 sentence denotes They carried varied contents of cytosine residues on the 3′-tail strand.
T7836 23621-23625 PRP denotes They
T7837 23626-23633 VBD denotes carried
T7838 23634-23640 VBN denotes varied
T7839 23641-23649 NNS denotes contents
T7840 23650-23652 IN denotes of
T7841 23653-23661 NN denotes cytosine
T7842 23662-23670 NNS denotes residues
T7843 23671-23673 IN denotes on
T7844 23674-23677 DT denotes the
T7846 23678-23679 CD denotes 3
T7848 23679-23680 SYM denotes ′
T7849 23680-23681 HYPH denotes -
T7847 23681-23685 NN denotes tail
T7845 23686-23692 NN denotes strand
T7850 23692-23693 . denotes .
T7851 23693-23870 sentence denotes In gel shift assays, Mcm4/6/7 bound to these Y-fork substrates with identical affinity (Figure 8A), consistent with the notion that the Mcm4/6/7 binds to single-stranded tails.
T7852 23694-23696 IN denotes In
T7854 23697-23700 NN denotes gel
T7855 23701-23706 NN denotes shift
T7856 23707-23713 NNS denotes assays
T7857 23713-23715 , denotes ,
T7858 23715-23719 NN denotes Mcm4
T7859 23719-23720 HYPH denotes /
T7860 23720-23721 CD denotes 6
T7861 23721-23722 HYPH denotes /
T7862 23722-23723 CD denotes 7
T7853 23724-23729 VBD denotes bound
T7863 23730-23732 IN denotes to
T7864 23733-23738 DT denotes these
T7866 23739-23740 NN denotes Y
T7868 23740-23741 HYPH denotes -
T7867 23741-23745 NN denotes fork
T7865 23746-23756 NNS denotes substrates
T7869 23757-23761 IN denotes with
T7870 23762-23771 JJ denotes identical
T7871 23772-23780 NN denotes affinity
T7872 23781-23782 -LRB- denotes (
T7874 23782-23788 NN denotes Figure
T7873 23789-23791 NN denotes 8A
T7875 23791-23792 -RRB- denotes )
T7876 23792-23794 , denotes ,
T7877 23794-23804 JJ denotes consistent
T7878 23805-23809 IN denotes with
T7879 23810-23813 DT denotes the
T7880 23814-23820 NN denotes notion
T7881 23821-23825 IN denotes that
T7883 23826-23829 DT denotes the
T7884 23830-23834 NN denotes Mcm4
T7885 23834-23835 HYPH denotes /
T7886 23835-23836 CD denotes 6
T7887 23836-23837 HYPH denotes /
T7888 23837-23838 CD denotes 7
T7882 23839-23844 VBZ denotes binds
T7889 23845-23847 IN denotes to
T7890 23848-23854 JJ denotes single
T7892 23854-23855 HYPH denotes -
T7891 23855-23863 VBN denotes stranded
T7893 23864-23869 NNS denotes tails
T7894 23869-23870 . denotes .
T7895 23870-24022 sentence denotes However, in DNA helicase assays, T-fork/(C:G)49 was hardly displaced by Mcm4/6/7, but was readily displaced by SV40 T-antigen DNA helicase (Figure 8B).
T7896 23871-23878 RB denotes However
T7898 23878-23880 , denotes ,
T7899 23880-23882 IN denotes in
T7900 23883-23886 NN denotes DNA
T7901 23887-23895 NN denotes helicase
T7902 23896-23902 NNS denotes assays
T7903 23902-23904 , denotes ,
T7904 23904-23905 NN denotes T
T7906 23905-23906 HYPH denotes -
T7905 23906-23910 NN denotes fork
T7907 23910-23911 HYPH denotes /
T7908 23911-23912 -LRB- denotes (
T7910 23912-23913 NN denotes C
T7911 23913-23914 : denotes :
T7909 23914-23915 NN denotes G
T7912 23915-23916 -RRB- denotes )
T7913 23916-23918 CD denotes 49
T7914 23919-23922 VBD denotes was
T7915 23923-23929 RB denotes hardly
T7897 23930-23939 VBN denotes displaced
T7916 23940-23942 IN denotes by
T7917 23943-23947 NN denotes Mcm4
T7918 23947-23948 HYPH denotes /
T7919 23948-23949 CD denotes 6
T7920 23949-23950 HYPH denotes /
T7921 23950-23951 CD denotes 7
T7922 23951-23953 , denotes ,
T7923 23953-23956 CC denotes but
T7924 23957-23960 VBD denotes was
T7926 23961-23968 RB denotes readily
T7925 23969-23978 VBN denotes displaced
T7927 23979-23981 IN denotes by
T7928 23982-23986 NN denotes SV40
T7930 23987-23988 NN denotes T
T7932 23988-23989 HYPH denotes -
T7931 23989-23996 NN denotes antigen
T7933 23997-24000 NN denotes DNA
T7929 24001-24009 NN denotes helicase
T7934 24010-24011 -LRB- denotes (
T7936 24011-24017 NN denotes Figure
T7935 24018-24020 NN denotes 8B
T7937 24020-24021 -RRB- denotes )
T7938 24021-24022 . denotes .
T7939 24022-24188 sentence denotes When thymine or adenine is inserted as every third nucleotide (repeats of CCT or CCA), the extent of unwinding increased (T-fork/(CCT:GGA)16 and T-fork/(CCA:GGT)16).
T7940 24023-24027 WRB denotes When
T7942 24028-24035 NN denotes thymine
T7943 24036-24038 CC denotes or
T7944 24039-24046 NN denotes adenine
T7945 24047-24049 VBZ denotes is
T7941 24050-24058 VBN denotes inserted
T7947 24059-24061 IN denotes as
T7948 24062-24067 DT denotes every
T7950 24068-24073 JJ denotes third
T7949 24074-24084 NN denotes nucleotide
T7951 24085-24086 -LRB- denotes (
T7952 24086-24093 NNS denotes repeats
T7953 24094-24096 IN denotes of
T7954 24097-24100 NN denotes CCT
T7955 24101-24103 CC denotes or
T7956 24104-24107 NN denotes CCA
T7957 24107-24108 -RRB- denotes )
T7958 24108-24110 , denotes ,
T7959 24110-24113 DT denotes the
T7960 24114-24120 NN denotes extent
T7961 24121-24123 IN denotes of
T7962 24124-24133 NN denotes unwinding
T7946 24134-24143 VBD denotes increased
T7963 24144-24145 -LRB- denotes (
T7965 24145-24146 NN denotes T
T7967 24146-24147 HYPH denotes -
T7966 24147-24151 NN denotes fork
T7968 24151-24152 HYPH denotes /
T7969 24152-24153 -LRB- denotes (
T7970 24153-24156 NN denotes CCT
T7971 24156-24157 : denotes :
T7964 24157-24160 NN denotes GGA
T7972 24160-24161 -RRB- denotes )
T7973 24161-24163 CD denotes 16
T7974 24164-24167 CC denotes and
T7975 24168-24169 NN denotes T
T7977 24169-24170 HYPH denotes -
T7976 24170-24174 NN denotes fork
T7979 24174-24175 HYPH denotes /
T7980 24175-24176 -LRB- denotes (
T7981 24176-24179 NN denotes CCA
T7982 24179-24180 : denotes :
T7978 24180-24183 NN denotes GGT
T7983 24183-24184 -RRB- denotes )
T7984 24184-24186 CD denotes 16
T7985 24186-24187 -RRB- denotes )
T7986 24187-24188 . denotes .
T7987 24188-24393 sentence denotes The efficiency of unwinding is roughly correlated with the content of GC pairs in the duplex segment [T-fork/(CCAA:GGTT)12, T-fork/(CCTT:GGAA)12, T-fork/(CAAA:GTTT)12 and T-fork/(CTTT:GAAA)12; Figure 8B].
T7988 24189-24192 DT denotes The
T7989 24193-24203 NN denotes efficiency
T7991 24204-24206 IN denotes of
T7992 24207-24216 NN denotes unwinding
T7993 24217-24219 VBZ denotes is
T7994 24220-24227 RB denotes roughly
T7990 24228-24238 VBN denotes correlated
T7995 24239-24243 IN denotes with
T7996 24244-24247 DT denotes the
T7997 24248-24255 NN denotes content
T7998 24256-24258 IN denotes of
T7999 24259-24261 NN denotes GC
T8000 24262-24267 NNS denotes pairs
T8001 24268-24270 IN denotes in
T8002 24271-24274 DT denotes the
T8004 24275-24281 NN denotes duplex
T8003 24282-24289 NN denotes segment
T8005 24290-24291 -LRB- denotes [
T8007 24291-24292 NN denotes T
T8009 24292-24293 HYPH denotes -
T8008 24293-24297 NN denotes fork
T8010 24297-24298 HYPH denotes /
T8011 24298-24299 -LRB- denotes (
T8012 24299-24303 NN denotes CCAA
T8013 24303-24304 : denotes :
T8006 24304-24308 NN denotes GGTT
T8014 24308-24309 -RRB- denotes )
T8015 24309-24311 CD denotes 12
T8016 24311-24313 , denotes ,
T8017 24313-24314 NN denotes T
T8019 24314-24315 HYPH denotes -
T8018 24315-24319 NN denotes fork
T8021 24319-24320 HYPH denotes /
T8022 24320-24321 -LRB- denotes (
T8023 24321-24325 NN denotes CCTT
T8024 24325-24326 : denotes :
T8020 24326-24330 NN denotes GGAA
T8025 24330-24331 -RRB- denotes )
T8026 24331-24333 CD denotes 12
T8027 24333-24335 , denotes ,
T8028 24335-24336 NN denotes T
T8030 24336-24337 HYPH denotes -
T8029 24337-24341 NN denotes fork
T8032 24341-24342 HYPH denotes /
T8033 24342-24343 -LRB- denotes (
T8034 24343-24347 NN denotes CAAA
T8035 24347-24348 : denotes :
T8031 24348-24352 NN denotes GTTT
T8036 24352-24353 -RRB- denotes )
T8037 24353-24355 CD denotes 12
T8038 24356-24359 CC denotes and
T8039 24360-24361 NN denotes T
T8041 24361-24362 HYPH denotes -
T8040 24362-24366 NN denotes fork
T8043 24366-24367 HYPH denotes /
T8044 24367-24368 -LRB- denotes (
T8045 24368-24372 NN denotes CTTT
T8046 24372-24373 : denotes :
T8042 24373-24377 NN denotes GAAA
T8047 24377-24378 -RRB- denotes )
T8048 24378-24380 CD denotes 12
T8049 24380-24381 : denotes ;
T8051 24382-24388 NN denotes Figure
T8050 24389-24391 NN denotes 8B
T8052 24391-24392 -RRB- denotes ]
T8053 24392-24393 . denotes .
T8054 24393-24489 sentence denotes It appears that the duplex segment containing <50% GC pairs is efficiently unwound (Figure 8C).
T8055 24394-24396 PRP denotes It
T8056 24397-24404 VBZ denotes appears
T8057 24405-24409 IN denotes that
T8059 24410-24413 DT denotes the
T8061 24414-24420 NN denotes duplex
T8060 24421-24428 NN denotes segment
T8062 24429-24439 VBG denotes containing
T8063 24440-24441 SYM denotes <
T8064 24441-24443 CD denotes 50
T8065 24443-24444 NN denotes %
T8067 24445-24447 NN denotes GC
T8066 24448-24453 NNS denotes pairs
T8068 24454-24456 VBZ denotes is
T8069 24457-24468 RB denotes efficiently
T8058 24469-24476 VBN denotes unwound
T8070 24477-24478 -LRB- denotes (
T8072 24478-24484 NN denotes Figure
T8071 24485-24487 NN denotes 8C
T8073 24487-24488 -RRB- denotes )
T8074 24488-24489 . denotes .
T8075 24489-24602 sentence denotes We next examined the effect of GC-rich segments on a partial heteroduplex DNA on a single-stranded circular DNA.
T8076 24490-24492 PRP denotes We
T8078 24493-24497 RB denotes next
T8077 24498-24506 VBD denotes examined
T8079 24507-24510 DT denotes the
T8080 24511-24517 NN denotes effect
T8081 24518-24520 IN denotes of
T8082 24521-24523 NN denotes GC
T8084 24523-24524 HYPH denotes -
T8083 24524-24528 JJ denotes rich
T8085 24529-24537 NNS denotes segments
T8086 24538-24540 IN denotes on
T8087 24541-24542 DT denotes a
T8089 24543-24550 JJ denotes partial
T8090 24551-24563 NN denotes heteroduplex
T8088 24564-24567 NN denotes DNA
T8091 24568-24570 IN denotes on
T8092 24571-24572 DT denotes a
T8094 24573-24579 JJ denotes single
T8096 24579-24580 HYPH denotes -
T8095 24580-24588 VBN denotes stranded
T8097 24589-24597 JJ denotes circular
T8093 24598-24601 NN denotes DNA
T8098 24601-24602 . denotes .
T8099 24602-24860 sentence denotes We have constructed two sets of 5′-tailed partial heteroduplex DNA substrates containing duplex regions of variable lengths; one on M13mp18 vector and the other on M13mp19 containing a 66 nt long G-rich segment downstream of the hybridizing oligonucleotide.
T8100 24603-24605 PRP denotes We
T8102 24606-24610 VBP denotes have
T8101 24611-24622 VBN denotes constructed
T8103 24623-24626 CD denotes two
T8104 24627-24631 NNS denotes sets
T8105 24632-24634 IN denotes of
T8106 24635-24636 CD denotes 5
T8108 24636-24637 SYM denotes ′
T8109 24637-24638 HYPH denotes -
T8107 24638-24644 VBN denotes tailed
T8111 24645-24652 JJ denotes partial
T8112 24653-24665 NN denotes heteroduplex
T8113 24666-24669 NN denotes DNA
T8110 24670-24680 NNS denotes substrates
T8114 24681-24691 VBG denotes containing
T8115 24692-24698 NN denotes duplex
T8116 24699-24706 NNS denotes regions
T8117 24707-24709 IN denotes of
T8118 24710-24718 JJ denotes variable
T8119 24719-24726 NNS denotes lengths
T8120 24726-24727 : denotes ;
T8121 24728-24731 CD denotes one
T8122 24732-24734 IN denotes on
T8123 24735-24742 NN denotes M13mp18
T8124 24743-24749 NN denotes vector
T8125 24750-24753 CC denotes and
T8126 24754-24757 DT denotes the
T8127 24758-24763 JJ denotes other
T8128 24764-24766 IN denotes on
T8129 24767-24774 NN denotes M13mp19
T8130 24775-24785 VBG denotes containing
T8131 24786-24787 DT denotes a
T8133 24788-24790 CD denotes 66
T8134 24791-24793 NN denotes nt
T8135 24794-24798 JJ denotes long
T8136 24799-24800 NN denotes G
T8138 24800-24801 HYPH denotes -
T8137 24801-24805 JJ denotes rich
T8132 24806-24813 NN denotes segment
T8139 24814-24824 RB denotes downstream
T8140 24825-24827 IN denotes of
T8141 24828-24831 DT denotes the
T8143 24832-24843 VBG denotes hybridizing
T8142 24844-24859 NN denotes oligonucleotide
T8144 24859-24860 . denotes .
T8145 24860-25031 sentence denotes We found that the Mcm4/6/7 helicase can displace duplex DNA up to 350 nt long on its own on the former substrate (Figure 8D, left), consistent with previous results (13).
T8146 24861-24863 PRP denotes We
T8147 24864-24869 VBD denotes found
T8148 24870-24874 IN denotes that
T8150 24875-24878 DT denotes the
T8152 24879-24883 NN denotes Mcm4
T8153 24883-24884 HYPH denotes /
T8154 24884-24885 CD denotes 6
T8155 24885-24886 HYPH denotes /
T8156 24886-24887 CD denotes 7
T8151 24888-24896 NN denotes helicase
T8157 24897-24900 MD denotes can
T8149 24901-24909 VB denotes displace
T8158 24910-24916 NN denotes duplex
T8159 24917-24920 NN denotes DNA
T8160 24921-24923 IN denotes up
T8162 24924-24926 IN denotes to
T8161 24927-24930 CD denotes 350
T8163 24931-24933 NN denotes nt
T8164 24934-24938 JJ denotes long
T8165 24939-24941 IN denotes on
T8166 24942-24945 PRP$ denotes its
T8167 24946-24949 JJ denotes own
T8168 24950-24952 IN denotes on
T8169 24953-24956 DT denotes the
T8171 24957-24963 JJ denotes former
T8170 24964-24973 NN denotes substrate
T8172 24974-24975 -LRB- denotes (
T8174 24975-24981 NN denotes Figure
T8173 24982-24984 NN denotes 8D
T8175 24984-24986 , denotes ,
T8176 24986-24990 JJ denotes left
T8177 24990-24991 -RRB- denotes )
T8178 24991-24993 , denotes ,
T8179 24993-25003 JJ denotes consistent
T8180 25004-25008 IN denotes with
T8181 25009-25017 JJ denotes previous
T8182 25018-25025 NNS denotes results
T8183 25026-25027 -LRB- denotes (
T8184 25027-25029 CD denotes 13
T8185 25029-25030 -RRB- denotes )
T8186 25030-25031 . denotes .
T8187 25031-25278 sentence denotes In contrast, displacement was inhibited over the GC-rich segments on the latter substrate (Figure 8D, right panel), although displacement up to 250 nt in length, albeit at a reduced level, was observed at a high concentration of Mcm4/6/7 complex.
T8188 25032-25034 IN denotes In
T8190 25035-25043 NN denotes contrast
T8191 25043-25045 , denotes ,
T8192 25045-25057 NN denotes displacement
T8193 25058-25061 VBD denotes was
T8189 25062-25071 VBN denotes inhibited
T8194 25072-25076 IN denotes over
T8195 25077-25080 DT denotes the
T8197 25081-25083 NN denotes GC
T8199 25083-25084 HYPH denotes -
T8198 25084-25088 JJ denotes rich
T8196 25089-25097 NNS denotes segments
T8200 25098-25100 IN denotes on
T8201 25101-25104 DT denotes the
T8203 25105-25111 JJ denotes latter
T8202 25112-25121 NN denotes substrate
T8204 25122-25123 -LRB- denotes (
T8206 25123-25129 NN denotes Figure
T8207 25130-25132 NN denotes 8D
T8208 25132-25134 , denotes ,
T8209 25134-25139 JJ denotes right
T8205 25140-25145 NN denotes panel
T8210 25145-25146 -RRB- denotes )
T8211 25146-25148 , denotes ,
T8212 25148-25156 IN denotes although
T8214 25157-25169 NN denotes displacement
T8215 25170-25172 IN denotes up
T8217 25173-25175 IN denotes to
T8216 25176-25179 CD denotes 250
T8218 25180-25182 NN denotes nt
T8219 25183-25185 IN denotes in
T8220 25186-25192 NN denotes length
T8221 25192-25194 , denotes ,
T8222 25194-25200 IN denotes albeit
T8223 25201-25203 IN denotes at
T8224 25204-25205 DT denotes a
T8226 25206-25213 VBN denotes reduced
T8225 25214-25219 NN denotes level
T8227 25219-25221 , denotes ,
T8228 25221-25224 VBD denotes was
T8213 25225-25233 VBN denotes observed
T8229 25234-25236 IN denotes at
T8230 25237-25238 DT denotes a
T8232 25239-25243 JJ denotes high
T8231 25244-25257 NN denotes concentration
T8233 25258-25260 IN denotes of
T8234 25261-25265 NN denotes Mcm4
T8236 25265-25266 HYPH denotes /
T8237 25266-25267 CD denotes 6
T8238 25267-25268 HYPH denotes /
T8239 25268-25269 CD denotes 7
T8235 25270-25277 NN denotes complex
T8240 25277-25278 . denotes .
T8241 25278-25415 sentence denotes This result is consistent with that on Y-fork and indicates that GC-rich duplex segment is inhibitory for unwinding by the Mcm helicase.
T8242 25279-25283 DT denotes This
T8243 25284-25290 NN denotes result
T8244 25291-25293 VBZ denotes is
T8245 25294-25304 JJ denotes consistent
T8246 25305-25309 IN denotes with
T8247 25310-25314 DT denotes that
T8248 25315-25317 IN denotes on
T8249 25318-25319 NN denotes Y
T8251 25319-25320 HYPH denotes -
T8250 25320-25324 NN denotes fork
T8252 25325-25328 CC denotes and
T8253 25329-25338 VBZ denotes indicates
T8254 25339-25343 IN denotes that
T8256 25344-25346 NN denotes GC
T8258 25346-25347 HYPH denotes -
T8257 25347-25351 JJ denotes rich
T8260 25352-25358 NN denotes duplex
T8259 25359-25366 NN denotes segment
T8255 25367-25369 VBZ denotes is
T8261 25370-25380 JJ denotes inhibitory
T8262 25381-25384 IN denotes for
T8263 25385-25394 NN denotes unwinding
T8264 25395-25397 IN denotes by
T8265 25398-25401 DT denotes the
T8267 25402-25405 NN denotes Mcm
T8266 25406-25414 NN denotes helicase
T8268 25414-25415 . denotes .
T8269 25415-25580 sentence denotes These results may indicate that the presence of thymine–adenine pairs with a certain frequency in the duplex region may facilitate continuous unwinding by Mcm4/6/7.
T8270 25416-25421 DT denotes These
T8271 25422-25429 NNS denotes results
T8273 25430-25433 MD denotes may
T8272 25434-25442 VB denotes indicate
T8274 25443-25447 IN denotes that
T8276 25448-25451 DT denotes the
T8277 25452-25460 NN denotes presence
T8278 25461-25463 IN denotes of
T8279 25464-25471 NN denotes thymine
T8281 25471-25472 HYPH denotes –
T8280 25472-25479 NN denotes adenine
T8282 25480-25485 NNS denotes pairs
T8283 25486-25490 IN denotes with
T8284 25491-25492 DT denotes a
T8286 25493-25500 JJ denotes certain
T8285 25501-25510 NN denotes frequency
T8287 25511-25513 IN denotes in
T8288 25514-25517 DT denotes the
T8290 25518-25524 NN denotes duplex
T8289 25525-25531 NN denotes region
T8291 25532-25535 MD denotes may
T8275 25536-25546 VB denotes facilitate
T8292 25547-25557 JJ denotes continuous
T8293 25558-25567 NN denotes unwinding
T8294 25568-25570 IN denotes by
T8295 25571-25575 NN denotes Mcm4
T8296 25575-25576 HYPH denotes /
T8297 25576-25577 CD denotes 6
T8298 25577-25578 HYPH denotes /
T8299 25578-25579 CD denotes 7
T8300 25579-25580 . denotes .
T8301 25580-25689 sentence denotes Alternatively, Mcm4/6/7 is not efficient enough to displace thermodynamically stable GC-base-paired segment.
T8302 25581-25594 RB denotes Alternatively
T8304 25594-25596 , denotes ,
T8305 25596-25600 NN denotes Mcm4
T8306 25600-25601 HYPH denotes /
T8307 25601-25602 CD denotes 6
T8308 25602-25603 HYPH denotes /
T8309 25603-25604 CD denotes 7
T8303 25605-25607 VBZ denotes is
T8310 25608-25611 RB denotes not
T8311 25612-25621 JJ denotes efficient
T8312 25622-25628 RB denotes enough
T8313 25629-25631 TO denotes to
T8314 25632-25640 VB denotes displace
T8315 25641-25658 RB denotes thermodynamically
T8316 25659-25665 JJ denotes stable
T8318 25666-25668 NN denotes GC
T8320 25668-25669 HYPH denotes -
T8321 25669-25673 NN denotes base
T8322 25673-25674 HYPH denotes -
T8319 25674-25680 VBN denotes paired
T8317 25681-25688 NN denotes segment
T8323 25688-25689 . denotes .
T8324 25689-25834 sentence denotes In order to distinguish these two possibilities, we designed a new T-fork substrate containing inosine (I) residue instead of guanosine residue.
T8325 25690-25692 IN denotes In
T8327 25693-25698 NN denotes order
T8328 25699-25701 TO denotes to
T8329 25702-25713 VB denotes distinguish
T8330 25714-25719 DT denotes these
T8332 25720-25723 CD denotes two
T8331 25724-25737 NNS denotes possibilities
T8333 25737-25739 , denotes ,
T8334 25739-25741 PRP denotes we
T8326 25742-25750 VBD denotes designed
T8335 25751-25752 DT denotes a
T8337 25753-25756 JJ denotes new
T8338 25757-25758 NN denotes T
T8340 25758-25759 HYPH denotes -
T8339 25759-25763 NN denotes fork
T8336 25764-25773 NN denotes substrate
T8341 25774-25784 VBG denotes containing
T8342 25785-25792 NN denotes inosine
T8344 25793-25794 -LRB- denotes (
T8345 25794-25795 NN denotes I
T8346 25795-25796 -RRB- denotes )
T8343 25797-25804 NN denotes residue
T8347 25805-25812 RB denotes instead
T8348 25813-25815 IN denotes of
T8349 25816-25825 NN denotes guanosine
T8350 25826-25833 NN denotes residue
T8351 25833-25834 . denotes .
T8352 25834-26007 sentence denotes The thermal stability of the I:C base pair is lower than that of the G:C base pair in duplex DNA due to loss of one hydrogen bond and is even lower than A:T base pair (31).
T8353 25835-25838 DT denotes The
T8355 25839-25846 JJ denotes thermal
T8354 25847-25856 NN denotes stability
T8357 25857-25859 IN denotes of
T8358 25860-25863 DT denotes the
T8360 25864-25865 NN denotes I
T8362 25865-25866 : denotes :
T8361 25866-25867 NN denotes C
T8363 25868-25872 NN denotes base
T8359 25873-25877 NN denotes pair
T8356 25878-25880 VBZ denotes is
T8364 25881-25886 JJR denotes lower
T8365 25887-25891 IN denotes than
T8366 25892-25896 DT denotes that
T8367 25897-25899 IN denotes of
T8368 25900-25903 DT denotes the
T8370 25904-25905 NN denotes G
T8372 25905-25906 : denotes :
T8371 25906-25907 NN denotes C
T8373 25908-25912 NN denotes base
T8369 25913-25917 NN denotes pair
T8374 25918-25920 IN denotes in
T8375 25921-25927 NN denotes duplex
T8376 25928-25931 NN denotes DNA
T8377 25932-25935 IN denotes due
T8378 25936-25938 IN denotes to
T8379 25939-25943 NN denotes loss
T8380 25944-25946 IN denotes of
T8381 25947-25950 CD denotes one
T8383 25951-25959 NN denotes hydrogen
T8382 25960-25964 NN denotes bond
T8384 25965-25968 CC denotes and
T8385 25969-25971 VBZ denotes is
T8386 25972-25976 RB denotes even
T8387 25977-25982 JJR denotes lower
T8388 25983-25987 IN denotes than
T8389 25988-25989 NN denotes A
T8391 25989-25990 : denotes :
T8390 25990-25991 NN denotes T
T8393 25992-25996 NN denotes base
T8392 25997-26001 NN denotes pair
T8394 26002-26003 -LRB- denotes (
T8395 26003-26005 CD denotes 31
T8396 26005-26006 -RRB- denotes )
T8397 26006-26007 . denotes .
T8398 26007-26138 sentence denotes We constructed the T-tailed fork substrates containing the (GCC:CGG)10, (GCC:CIG)10 or (GAA:CTT)10 duplex DNA segment (Figure 8E).
T8399 26008-26010 PRP denotes We
T8400 26011-26022 VBD denotes constructed
T8401 26023-26026 DT denotes the
T8403 26027-26028 NN denotes T
T8405 26028-26029 HYPH denotes -
T8404 26029-26035 VBN denotes tailed
T8406 26036-26040 NN denotes fork
T8402 26041-26051 NNS denotes substrates
T8407 26052-26062 VBG denotes containing
T8408 26063-26066 DT denotes the
T8410 26067-26068 -LRB- denotes (
T8412 26068-26071 NN denotes GCC
T8413 26071-26072 : denotes :
T8411 26072-26075 NN denotes CGG
T8414 26075-26076 -RRB- denotes )
T8415 26076-26078 CD denotes 10
T8416 26078-26080 , denotes ,
T8417 26080-26081 -LRB- denotes (
T8419 26081-26084 NN denotes GCC
T8420 26084-26085 : denotes :
T8418 26085-26088 NN denotes CIG
T8421 26088-26089 -RRB- denotes )
T8422 26089-26091 CD denotes 10
T8423 26092-26094 CC denotes or
T8424 26095-26096 -LRB- denotes (
T8426 26096-26099 NN denotes GAA
T8427 26099-26100 : denotes :
T8425 26100-26103 NN denotes CTT
T8428 26103-26104 -RRB- denotes )
T8429 26104-26106 CD denotes 10
T8430 26107-26113 NN denotes duplex
T8431 26114-26117 NN denotes DNA
T8409 26118-26125 NN denotes segment
T8432 26126-26127 -LRB- denotes (
T8434 26127-26133 NN denotes Figure
T8433 26134-26136 NN denotes 8E
T8435 26136-26137 -RRB- denotes )
T8436 26137-26138 . denotes .
T8437 26138-26284 sentence denotes Mcm4/6/7 hardly displaced the 31 nt long duplex of GCC:CGG repeats, whereas the T-fork/(GAA:CTT)10 was displaced efficiently, as described above.
T8438 26139-26143 NN denotes Mcm4
T8440 26143-26144 HYPH denotes /
T8441 26144-26145 CD denotes 6
T8442 26145-26146 HYPH denotes /
T8443 26146-26147 CD denotes 7
T8444 26148-26154 RB denotes hardly
T8439 26155-26164 VBD denotes displaced
T8445 26165-26168 DT denotes the
T8447 26169-26171 CD denotes 31
T8448 26172-26174 NN denotes nt
T8449 26175-26179 JJ denotes long
T8446 26180-26186 NN denotes duplex
T8450 26187-26189 IN denotes of
T8451 26190-26193 NN denotes GCC
T8453 26193-26194 : denotes :
T8452 26194-26197 NN denotes CGG
T8454 26198-26205 NNS denotes repeats
T8455 26205-26207 , denotes ,
T8456 26207-26214 IN denotes whereas
T8458 26215-26218 DT denotes the
T8460 26219-26220 NN denotes T
T8462 26220-26221 HYPH denotes -
T8461 26221-26225 NN denotes fork
T8463 26225-26226 HYPH denotes /
T8464 26226-26227 -LRB- denotes (
T8465 26227-26230 NN denotes GAA
T8466 26230-26231 : denotes :
T8459 26231-26234 NN denotes CTT
T8467 26234-26235 -RRB- denotes )
T8468 26235-26237 CD denotes 10
T8469 26238-26241 VBD denotes was
T8457 26242-26251 VBN denotes displaced
T8470 26252-26263 RB denotes efficiently
T8471 26263-26265 , denotes ,
T8472 26265-26267 IN denotes as
T8473 26268-26277 VBN denotes described
T8474 26278-26283 RB denotes above
T8475 26283-26284 . denotes .
T8476 26284-26533 sentence denotes T-fork/(GCC:CIG)10 was displaced with efficiency much greater than that of T-fork/(GCC:CGG)10, indicating that the thermostability of the duplex segment, not the lack of AT base pair, is responsible for inability of Mcm4/6/7 to displace the duplex.
T8477 26285-26286 NN denotes T
T8479 26286-26287 HYPH denotes -
T8478 26287-26291 NN denotes fork
T8481 26291-26292 HYPH denotes /
T8482 26292-26293 -LRB- denotes (
T8483 26293-26296 NN denotes GCC
T8484 26296-26297 : denotes :
T8480 26297-26300 NN denotes CIG
T8486 26300-26301 -RRB- denotes )
T8487 26301-26303 CD denotes 10
T8488 26304-26307 VBD denotes was
T8485 26308-26317 VBN denotes displaced
T8489 26318-26322 IN denotes with
T8490 26323-26333 NN denotes efficiency
T8491 26334-26338 RB denotes much
T8492 26339-26346 JJR denotes greater
T8493 26347-26351 IN denotes than
T8494 26352-26356 DT denotes that
T8495 26357-26359 IN denotes of
T8496 26360-26361 NN denotes T
T8498 26361-26362 HYPH denotes -
T8497 26362-26366 NN denotes fork
T8500 26366-26367 HYPH denotes /
T8501 26367-26368 -LRB- denotes (
T8502 26368-26371 NN denotes GCC
T8503 26371-26372 : denotes :
T8499 26372-26375 NN denotes CGG
T8504 26375-26376 -RRB- denotes )
T8505 26376-26378 CD denotes 10
T8506 26378-26380 , denotes ,
T8507 26380-26390 VBG denotes indicating
T8508 26391-26395 IN denotes that
T8510 26396-26399 DT denotes the
T8511 26400-26415 NN denotes thermostability
T8512 26416-26418 IN denotes of
T8513 26419-26422 DT denotes the
T8515 26423-26429 NN denotes duplex
T8514 26430-26437 NN denotes segment
T8516 26437-26439 , denotes ,
T8517 26439-26442 CC denotes not
T8518 26443-26446 DT denotes the
T8519 26447-26451 NN denotes lack
T8520 26452-26454 IN denotes of
T8521 26455-26457 NN denotes AT
T8523 26458-26462 NN denotes base
T8522 26463-26467 NN denotes pair
T8524 26467-26469 , denotes ,
T8509 26469-26471 VBZ denotes is
T8525 26472-26483 JJ denotes responsible
T8526 26484-26487 IN denotes for
T8527 26488-26497 NN denotes inability
T8528 26498-26500 IN denotes of
T8529 26501-26505 NN denotes Mcm4
T8530 26505-26506 HYPH denotes /
T8531 26506-26507 CD denotes 6
T8532 26507-26508 HYPH denotes /
T8533 26508-26509 CD denotes 7
T8534 26510-26512 TO denotes to
T8535 26513-26521 VB denotes displace
T8536 26522-26525 DT denotes the
T8537 26526-26532 NN denotes duplex
T8538 26532-26533 . denotes .
T8790 26546-26549 NN denotes DNA
T8791 26550-26558 NN denotes helicase
T8792 26559-26561 VBZ denotes is
T8793 26562-26563 DT denotes a
T8795 26564-26571 JJ denotes central
T8794 26572-26578 NN denotes factor
T8796 26579-26582 IN denotes for
T8797 26583-26592 NN denotes operation
T8798 26593-26595 IN denotes of
T8799 26596-26607 NN denotes replication
T8800 26608-26613 NNS denotes forks
T8801 26613-26614 . denotes .
T8802 26614-26694 sentence denotes It also plays a crucial role in the initiation step at the replication origins.
T8803 26615-26617 PRP denotes It
T8805 26618-26622 RB denotes also
T8804 26623-26628 VBZ denotes plays
T8806 26629-26630 DT denotes a
T8808 26631-26638 JJ denotes crucial
T8807 26639-26643 NN denotes role
T8809 26644-26646 IN denotes in
T8810 26647-26650 DT denotes the
T8812 26651-26661 NN denotes initiation
T8811 26662-26666 NN denotes step
T8813 26667-26669 IN denotes at
T8814 26670-26673 DT denotes the
T8816 26674-26685 NN denotes replication
T8815 26686-26693 NNS denotes origins
T8817 26693-26694 . denotes .
T8818 26694-26892 sentence denotes Therefore, the elucidation of how the replicative helicases interact with DNA substrate and how they are activated is crucial for understanding the molecular basis of initiation of DNA replication.
T8819 26695-26704 RB denotes Therefore
T8821 26704-26706 , denotes ,
T8822 26706-26709 DT denotes the
T8823 26710-26721 NN denotes elucidation
T8824 26722-26724 IN denotes of
T8825 26725-26728 WRB denotes how
T8827 26729-26732 DT denotes the
T8829 26733-26744 JJ denotes replicative
T8828 26745-26754 NNS denotes helicases
T8826 26755-26763 VBP denotes interact
T8830 26764-26768 IN denotes with
T8831 26769-26772 NN denotes DNA
T8832 26773-26782 NN denotes substrate
T8833 26783-26786 CC denotes and
T8834 26787-26790 WRB denotes how
T8836 26791-26795 PRP denotes they
T8837 26796-26799 VBP denotes are
T8835 26800-26809 VBN denotes activated
T8820 26810-26812 VBZ denotes is
T8838 26813-26820 JJ denotes crucial
T8839 26821-26824 IN denotes for
T8840 26825-26838 VBG denotes understanding
T8841 26839-26842 DT denotes the
T8843 26843-26852 JJ denotes molecular
T8842 26853-26858 NN denotes basis
T8844 26859-26861 IN denotes of
T8845 26862-26872 NN denotes initiation
T8846 26873-26875 IN denotes of
T8847 26876-26879 NN denotes DNA
T8848 26880-26891 NN denotes replication
T8849 26891-26892 . denotes .
T8850 26892-26981 sentence denotes It is now believed that Mcm is a major component of the eukaryotic replicative helicase.
T8851 26893-26895 PRP denotes It
T8853 26896-26898 VBZ denotes is
T8854 26899-26902 RB denotes now
T8852 26903-26911 VBN denotes believed
T8855 26912-26916 IN denotes that
T8857 26917-26920 NN denotes Mcm
T8856 26921-26923 VBZ denotes is
T8858 26924-26925 DT denotes a
T8860 26926-26931 JJ denotes major
T8859 26932-26941 NN denotes component
T8861 26942-26944 IN denotes of
T8862 26945-26948 DT denotes the
T8864 26949-26959 JJ denotes eukaryotic
T8865 26960-26971 JJ denotes replicative
T8863 26972-26980 NN denotes helicase
T8866 26980-26981 . denotes .
T8867 26981-27146 sentence denotes In this report, we have conducted detailed analyses on binding and helicase actions of mouse Mcm4/6/7 using various substrates including fork and bubble structures.
T8868 26982-26984 IN denotes In
T8870 26985-26989 DT denotes this
T8871 26990-26996 NN denotes report
T8872 26996-26998 , denotes ,
T8873 26998-27000 PRP denotes we
T8874 27001-27005 VBP denotes have
T8869 27006-27015 VBN denotes conducted
T8875 27016-27024 JJ denotes detailed
T8876 27025-27033 NNS denotes analyses
T8877 27034-27036 IN denotes on
T8878 27037-27044 NN denotes binding
T8880 27045-27048 CC denotes and
T8881 27049-27057 NN denotes helicase
T8879 27058-27065 NNS denotes actions
T8882 27066-27068 IN denotes of
T8883 27069-27074 NN denotes mouse
T8884 27075-27079 NN denotes Mcm4
T8885 27079-27080 HYPH denotes /
T8886 27080-27081 CD denotes 6
T8887 27081-27082 HYPH denotes /
T8888 27082-27083 CD denotes 7
T8889 27084-27089 VBG denotes using
T8890 27090-27097 JJ denotes various
T8891 27098-27108 NNS denotes substrates
T8892 27109-27118 VBG denotes including
T8893 27119-27123 NN denotes fork
T8895 27124-27127 CC denotes and
T8896 27128-27134 NN denotes bubble
T8894 27135-27145 NNS denotes structures
T8897 27145-27146 . denotes .
T9088 27148-27153 NNS denotes Modes
T9089 27154-27156 IN denotes of
T9090 27157-27164 NN denotes binding
T9091 27165-27168 CC denotes and
T9092 27169-27179 NN denotes activation
T9093 27180-27182 IN denotes of
T9094 27183-27187 NN denotes Mcm4
T9096 27187-27188 HYPH denotes /
T9097 27188-27189 CD denotes 6
T9098 27189-27190 HYPH denotes /
T9099 27190-27191 CD denotes 7
T9095 27192-27200 NN denotes helicase
T9100 27201-27203 IN denotes on
T9101 27204-27205 DT denotes a
T9103 27206-27212 NN denotes bubble
T9102 27213-27222 NN denotes structure
T9104 27222-27329 sentence denotes Initiation of DNA replication is associated with localized melting of duplex DNA near replication origins.
T9105 27223-27233 NN denotes Initiation
T9107 27234-27236 IN denotes of
T9108 27237-27240 NN denotes DNA
T9109 27241-27252 NN denotes replication
T9110 27253-27255 VBZ denotes is
T9106 27256-27266 VBN denotes associated
T9111 27267-27271 IN denotes with
T9112 27272-27281 VBN denotes localized
T9113 27282-27289 NN denotes melting
T9114 27290-27292 IN denotes of
T9115 27293-27299 NN denotes duplex
T9116 27300-27303 NN denotes DNA
T9117 27304-27308 JJ denotes near
T9118 27309-27320 NN denotes replication
T9119 27321-27328 NNS denotes origins
T9120 27328-27329 . denotes .
T9121 27329-27448 sentence denotes Helicases are loaded onto replication forks through the melted region, induced by initiator binding, in bacteria (32).
T9122 27330-27339 NNS denotes Helicases
T9124 27340-27343 VBP denotes are
T9123 27344-27350 VBN denotes loaded
T9125 27351-27355 IN denotes onto
T9126 27356-27367 NN denotes replication
T9127 27368-27373 NNS denotes forks
T9128 27374-27381 IN denotes through
T9129 27382-27385 DT denotes the
T9131 27386-27392 VBN denotes melted
T9130 27393-27399 NN denotes region
T9132 27399-27401 , denotes ,
T9133 27401-27408 VBN denotes induced
T9134 27409-27411 IN denotes by
T9135 27412-27421 NN denotes initiator
T9136 27422-27429 NN denotes binding
T9137 27429-27431 , denotes ,
T9138 27431-27433 IN denotes in
T9139 27434-27442 NNS denotes bacteria
T9140 27443-27444 -LRB- denotes (
T9141 27444-27446 CD denotes 32
T9142 27446-27447 -RRB- denotes )
T9143 27447-27448 . denotes .
T9144 27448-27579 sentence denotes We previously reported that Mcm4/6/7 can be loaded onto a bubble-like structure and can serve as a DNA helicase at the forks (13).
T9145 27449-27451 PRP denotes We
T9147 27452-27462 RB denotes previously
T9146 27463-27471 VBD denotes reported
T9148 27472-27476 IN denotes that
T9150 27477-27481 NN denotes Mcm4
T9151 27481-27482 HYPH denotes /
T9152 27482-27483 CD denotes 6
T9153 27483-27484 HYPH denotes /
T9154 27484-27485 CD denotes 7
T9155 27486-27489 MD denotes can
T9156 27490-27492 VB denotes be
T9149 27493-27499 VBN denotes loaded
T9157 27500-27504 IN denotes onto
T9158 27505-27506 DT denotes a
T9160 27507-27513 NN denotes bubble
T9162 27513-27514 HYPH denotes -
T9161 27514-27518 JJ denotes like
T9159 27519-27528 NN denotes structure
T9163 27529-27532 CC denotes and
T9164 27533-27536 MD denotes can
T9165 27537-27542 VB denotes serve
T9166 27543-27545 IN denotes as
T9167 27546-27547 DT denotes a
T9169 27548-27551 NN denotes DNA
T9168 27552-27560 NN denotes helicase
T9170 27561-27563 IN denotes at
T9171 27564-27567 DT denotes the
T9172 27568-27573 NNS denotes forks
T9173 27574-27575 -LRB- denotes (
T9174 27575-27577 CD denotes 13
T9175 27577-27578 -RRB- denotes )
T9176 27578-27579 . denotes .
T9177 27579-27773 sentence denotes The ability of Mcm4/6/7 to unwind the bubble substrate but not the conventional duplex DNA (Figures 2 and 13) indicates that Mcm can be loaded through the single-stranded segment of the bubble.
T9178 27580-27583 DT denotes The
T9179 27584-27591 NN denotes ability
T9181 27592-27594 IN denotes of
T9182 27595-27599 NN denotes Mcm4
T9183 27599-27600 HYPH denotes /
T9184 27600-27601 CD denotes 6
T9185 27601-27602 HYPH denotes /
T9186 27602-27603 CD denotes 7
T9187 27604-27606 TO denotes to
T9188 27607-27613 VB denotes unwind
T9189 27614-27617 DT denotes the
T9191 27618-27624 NN denotes bubble
T9190 27625-27634 NN denotes substrate
T9192 27635-27638 CC denotes but
T9193 27639-27642 RB denotes not
T9194 27643-27646 DT denotes the
T9196 27647-27659 JJ denotes conventional
T9197 27660-27666 NN denotes duplex
T9195 27667-27670 NN denotes DNA
T9198 27671-27672 -LRB- denotes (
T9200 27672-27679 NNS denotes Figures
T9199 27680-27681 CD denotes 2
T9201 27682-27685 CC denotes and
T9202 27686-27688 CD denotes 13
T9203 27688-27689 -RRB- denotes )
T9180 27690-27699 VBZ denotes indicates
T9204 27700-27704 IN denotes that
T9206 27705-27708 NN denotes Mcm
T9207 27709-27712 MD denotes can
T9208 27713-27715 VB denotes be
T9205 27716-27722 VBN denotes loaded
T9209 27723-27730 IN denotes through
T9210 27731-27734 DT denotes the
T9212 27735-27741 JJ denotes single
T9214 27741-27742 HYPH denotes -
T9213 27742-27750 VBN denotes stranded
T9211 27751-27758 NN denotes segment
T9215 27759-27761 IN denotes of
T9216 27762-27765 DT denotes the
T9217 27766-27772 NN denotes bubble
T9218 27772-27773 . denotes .
T9219 27773-27926 sentence denotes Furthermore, the Mcm4/6/7 complex displays marked preference for thymine-rich sequences within the single-stranded segment for helicase activation (13).
T9220 27774-27785 RB denotes Furthermore
T9222 27785-27787 , denotes ,
T9223 27787-27790 DT denotes the
T9225 27791-27795 NN denotes Mcm4
T9226 27795-27796 HYPH denotes /
T9227 27796-27797 CD denotes 6
T9228 27797-27798 HYPH denotes /
T9229 27798-27799 CD denotes 7
T9224 27800-27807 NN denotes complex
T9221 27808-27816 VBZ denotes displays
T9230 27817-27823 JJ denotes marked
T9231 27824-27834 NN denotes preference
T9232 27835-27838 IN denotes for
T9233 27839-27846 NN denotes thymine
T9235 27846-27847 HYPH denotes -
T9234 27847-27851 JJ denotes rich
T9236 27852-27861 NNS denotes sequences
T9237 27862-27868 IN denotes within
T9238 27869-27872 DT denotes the
T9240 27873-27879 JJ denotes single
T9242 27879-27880 HYPH denotes -
T9241 27880-27888 VBN denotes stranded
T9239 27889-27896 NN denotes segment
T9243 27897-27900 IN denotes for
T9244 27901-27909 NN denotes helicase
T9245 27910-27920 NN denotes activation
T9246 27921-27922 -LRB- denotes (
T9247 27922-27924 CD denotes 13
T9248 27924-27925 -RRB- denotes )
T9249 27925-27926 . denotes .
T9250 27926-28234 sentence denotes The results in this report indicate that helicase action of Mcm4/6/7 on synthetic bubbles may depend on the presence of an unpaired region of sufficient length (at least 40 nt), which may permit assembly of a double-hexameric complex on the substrate DNA, similar to SV40 T-antigen protein (Figure 2A) (21).
T9251 27927-27930 DT denotes The
T9252 27931-27938 NNS denotes results
T9254 27939-27941 IN denotes in
T9255 27942-27946 DT denotes this
T9256 27947-27953 NN denotes report
T9253 27954-27962 VBP denotes indicate
T9257 27963-27967 IN denotes that
T9259 27968-27976 NN denotes helicase
T9260 27977-27983 NN denotes action
T9261 27984-27986 IN denotes of
T9262 27987-27991 NN denotes Mcm4
T9263 27991-27992 HYPH denotes /
T9264 27992-27993 CD denotes 6
T9265 27993-27994 HYPH denotes /
T9266 27994-27995 CD denotes 7
T9267 27996-27998 IN denotes on
T9268 27999-28008 JJ denotes synthetic
T9269 28009-28016 NNS denotes bubbles
T9270 28017-28020 MD denotes may
T9258 28021-28027 VB denotes depend
T9271 28028-28030 IN denotes on
T9272 28031-28034 DT denotes the
T9273 28035-28043 NN denotes presence
T9274 28044-28046 IN denotes of
T9275 28047-28049 DT denotes an
T9277 28050-28058 JJ denotes unpaired
T9276 28059-28065 NN denotes region
T9278 28066-28068 IN denotes of
T9279 28069-28079 JJ denotes sufficient
T9280 28080-28086 NN denotes length
T9281 28087-28088 -LRB- denotes (
T9283 28088-28090 RB denotes at
T9284 28091-28096 RBS denotes least
T9285 28097-28099 CD denotes 40
T9282 28100-28102 NN denotes nt
T9286 28102-28103 -RRB- denotes )
T9287 28103-28105 , denotes ,
T9288 28105-28110 WDT denotes which
T9290 28111-28114 MD denotes may
T9289 28115-28121 VB denotes permit
T9291 28122-28130 NN denotes assembly
T9292 28131-28133 IN denotes of
T9293 28134-28135 DT denotes a
T9295 28136-28142 JJ denotes double
T9297 28142-28143 HYPH denotes -
T9296 28143-28152 JJ denotes hexameric
T9294 28153-28160 NN denotes complex
T9298 28161-28163 IN denotes on
T9299 28164-28167 DT denotes the
T9301 28168-28177 NN denotes substrate
T9300 28178-28181 NN denotes DNA
T9302 28181-28183 , denotes ,
T9303 28183-28190 JJ denotes similar
T9304 28191-28193 IN denotes to
T9305 28194-28198 NN denotes SV40
T9307 28199-28200 NN denotes T
T9309 28200-28201 HYPH denotes -
T9308 28201-28208 NN denotes antigen
T9306 28209-28216 NN denotes protein
T9310 28217-28218 -LRB- denotes (
T9312 28218-28224 NN denotes Figure
T9311 28225-28227 NN denotes 2A
T9313 28227-28228 -RRB- denotes )
T9314 28229-28230 -LRB- denotes (
T9315 28230-28232 CD denotes 21
T9316 28232-28233 -RRB- denotes )
T9317 28233-28234 . denotes .
T9318 28234-28393 sentence denotes When one strand of the single-stranded segment in T-rich bubble was replaced by guanine-rich sequences, the efficiency of unwinding was significantly reduced.
T9319 28235-28239 WRB denotes When
T9321 28240-28243 CD denotes one
T9322 28244-28250 NN denotes strand
T9323 28251-28253 IN denotes of
T9324 28254-28257 DT denotes the
T9326 28258-28264 JJ denotes single
T9328 28264-28265 HYPH denotes -
T9327 28265-28273 VBN denotes stranded
T9325 28274-28281 NN denotes segment
T9329 28282-28284 IN denotes in
T9330 28285-28286 NN denotes T
T9332 28286-28287 HYPH denotes -
T9331 28287-28291 JJ denotes rich
T9333 28292-28298 NN denotes bubble
T9334 28299-28302 VBD denotes was
T9320 28303-28311 VBN denotes replaced
T9336 28312-28314 IN denotes by
T9337 28315-28322 NN denotes guanine
T9339 28322-28323 HYPH denotes -
T9338 28323-28327 JJ denotes rich
T9340 28328-28337 NNS denotes sequences
T9341 28337-28339 , denotes ,
T9342 28339-28342 DT denotes the
T9343 28343-28353 NN denotes efficiency
T9344 28354-28356 IN denotes of
T9345 28357-28366 NN denotes unwinding
T9346 28367-28370 VBD denotes was
T9347 28371-28384 RB denotes significantly
T9335 28385-28392 VBN denotes reduced
T9348 28392-28393 . denotes .
T9349 28393-28577 sentence denotes Our footprinting analyses showed that Mcm4/6/7 strongly protects 25 nt single-stranded DNA segment adjacent to each branch point and proximal to 5′ ends of both strands of the bubble.
T9350 28394-28397 PRP$ denotes Our
T9352 28398-28410 NN denotes footprinting
T9351 28411-28419 NNS denotes analyses
T9353 28420-28426 VBD denotes showed
T9354 28427-28431 IN denotes that
T9356 28432-28436 NN denotes Mcm4
T9357 28436-28437 HYPH denotes /
T9358 28437-28438 CD denotes 6
T9359 28438-28439 HYPH denotes /
T9360 28439-28440 CD denotes 7
T9361 28441-28449 RB denotes strongly
T9355 28450-28458 VBZ denotes protects
T9362 28459-28461 CD denotes 25
T9363 28462-28464 NN denotes nt
T9365 28465-28471 JJ denotes single
T9367 28471-28472 HYPH denotes -
T9366 28472-28480 VBN denotes stranded
T9368 28481-28484 NN denotes DNA
T9364 28485-28492 NN denotes segment
T9369 28493-28501 JJ denotes adjacent
T9370 28502-28504 IN denotes to
T9371 28505-28509 DT denotes each
T9373 28510-28516 NN denotes branch
T9372 28517-28522 NN denotes point
T9374 28523-28526 CC denotes and
T9375 28527-28535 JJ denotes proximal
T9376 28536-28538 IN denotes to
T9377 28539-28540 CD denotes 5
T9379 28540-28541 SYM denotes ′
T9378 28542-28546 NNS denotes ends
T9380 28547-28549 IN denotes of
T9381 28550-28554 DT denotes both
T9382 28555-28562 NNS denotes strands
T9383 28563-28565 IN denotes of
T9384 28566-28569 DT denotes the
T9385 28570-28576 NN denotes bubble
T9386 28576-28577 . denotes .
T9387 28577-28819 sentence denotes At each fork, one Mcm4/6/7 complex is likely to encircle the single-stranded DNA strand and two hexamers may bind symmetrically to the bubble substrate, forming a double hexameric structure on the bubble (see schematic drawing of Figure 1A).
T9388 28578-28580 IN denotes At
T9390 28581-28585 DT denotes each
T9391 28586-28590 NN denotes fork
T9392 28590-28592 , denotes ,
T9393 28592-28595 CD denotes one
T9395 28596-28600 NN denotes Mcm4
T9396 28600-28601 HYPH denotes /
T9397 28601-28602 CD denotes 6
T9398 28602-28603 HYPH denotes /
T9399 28603-28604 CD denotes 7
T9394 28605-28612 NN denotes complex
T9389 28613-28615 VBZ denotes is
T9400 28616-28622 JJ denotes likely
T9401 28623-28625 TO denotes to
T9402 28626-28634 VB denotes encircle
T9403 28635-28638 DT denotes the
T9405 28639-28645 JJ denotes single
T9407 28645-28646 HYPH denotes -
T9406 28646-28654 VBN denotes stranded
T9408 28655-28658 NN denotes DNA
T9404 28659-28665 NN denotes strand
T9409 28666-28669 CC denotes and
T9410 28670-28673 CD denotes two
T9411 28674-28682 NNS denotes hexamers
T9413 28683-28686 MD denotes may
T9412 28687-28691 VB denotes bind
T9414 28692-28705 RB denotes symmetrically
T9415 28706-28708 IN denotes to
T9416 28709-28712 DT denotes the
T9418 28713-28719 NN denotes bubble
T9417 28720-28729 NN denotes substrate
T9419 28729-28731 , denotes ,
T9420 28731-28738 VBG denotes forming
T9421 28739-28740 DT denotes a
T9423 28741-28747 JJ denotes double
T9424 28748-28757 JJ denotes hexameric
T9422 28758-28767 NN denotes structure
T9425 28768-28770 IN denotes on
T9426 28771-28774 DT denotes the
T9427 28775-28781 NN denotes bubble
T9428 28782-28783 -LRB- denotes (
T9429 28783-28786 VB denotes see
T9430 28787-28796 JJ denotes schematic
T9431 28797-28804 NN denotes drawing
T9432 28805-28807 IN denotes of
T9433 28808-28814 NN denotes Figure
T9434 28815-28817 NN denotes 1A
T9435 28817-28818 -RRB- denotes )
T9436 28818-28819 . denotes .
T9437 28819-29008 sentence denotes Efficient unwinding into both directions may require simultaneous activation of both hexamers which may sit at the center while extruding the unwound single-stranded DNA through the rings.
T9438 28820-28829 JJ denotes Efficient
T9439 28830-28839 NN denotes unwinding
T9441 28840-28844 IN denotes into
T9442 28845-28849 DT denotes both
T9443 28850-28860 NNS denotes directions
T9444 28861-28864 MD denotes may
T9440 28865-28872 VB denotes require
T9445 28873-28885 JJ denotes simultaneous
T9446 28886-28896 NN denotes activation
T9447 28897-28899 IN denotes of
T9448 28900-28904 DT denotes both
T9449 28905-28913 NNS denotes hexamers
T9450 28914-28919 WDT denotes which
T9452 28920-28923 MD denotes may
T9451 28924-28927 VB denotes sit
T9453 28928-28930 IN denotes at
T9454 28931-28934 DT denotes the
T9455 28935-28941 NN denotes center
T9456 28942-28947 IN denotes while
T9457 28948-28957 VBG denotes extruding
T9458 28958-28961 DT denotes the
T9460 28962-28969 JJ denotes unwound
T9461 28970-28976 JJ denotes single
T9463 28976-28977 HYPH denotes -
T9462 28977-28985 VBN denotes stranded
T9459 28986-28989 NN denotes DNA
T9464 28990-28997 IN denotes through
T9465 28998-29001 DT denotes the
T9466 29002-29007 NNS denotes rings
T9467 29007-29008 . denotes .
T9468 29008-29104 sentence denotes This may closely resemble the proposed modes of binding and helicase actions of T-antigen (21).
T9469 29009-29013 DT denotes This
T9471 29014-29017 MD denotes may
T9472 29018-29025 RB denotes closely
T9470 29026-29034 VB denotes resemble
T9473 29035-29038 DT denotes the
T9475 29039-29047 VBN denotes proposed
T9474 29048-29053 NNS denotes modes
T9476 29054-29056 IN denotes of
T9477 29057-29064 NN denotes binding
T9479 29065-29068 CC denotes and
T9480 29069-29077 NN denotes helicase
T9478 29078-29085 NNS denotes actions
T9481 29086-29088 IN denotes of
T9482 29089-29090 NN denotes T
T9484 29090-29091 HYPH denotes -
T9483 29091-29098 NN denotes antigen
T9485 29099-29100 -LRB- denotes (
T9486 29100-29102 CD denotes 21
T9487 29102-29103 -RRB- denotes )
T9488 29103-29104 . denotes .
T9489 29104-29482 sentence denotes Although we cannot totally exclude the possibility that one Mcm4/6/7 hexamer at each fork unwinds the duplex independently, ring-shaped structures of mouse and archea MCM, as revealed by electron microscopy, bear much similarity to the recently solved structure of the SV40 large T-antigen (4,6), and are in favor of the double-hexameric structure of mouse Mcm on a bubble DNA.
T9490 29105-29113 IN denotes Although
T9492 29114-29116 PRP denotes we
T9493 29117-29120 MD denotes can
T9494 29120-29123 RB denotes not
T9495 29124-29131 RB denotes totally
T9491 29132-29139 VB denotes exclude
T9497 29140-29143 DT denotes the
T9498 29144-29155 NN denotes possibility
T9499 29156-29160 IN denotes that
T9501 29161-29164 CD denotes one
T9503 29165-29169 NN denotes Mcm4
T9504 29169-29170 HYPH denotes /
T9505 29170-29171 CD denotes 6
T9506 29171-29172 HYPH denotes /
T9507 29172-29173 CD denotes 7
T9502 29174-29181 NN denotes hexamer
T9508 29182-29184 IN denotes at
T9509 29185-29189 DT denotes each
T9510 29190-29194 NN denotes fork
T9500 29195-29202 VBZ denotes unwinds
T9511 29203-29206 DT denotes the
T9512 29207-29213 NN denotes duplex
T9513 29214-29227 RB denotes independently
T9514 29227-29229 , denotes ,
T9515 29229-29233 NN denotes ring
T9517 29233-29234 HYPH denotes -
T9516 29234-29240 VBN denotes shaped
T9518 29241-29251 NNS denotes structures
T9519 29252-29254 IN denotes of
T9520 29255-29260 NN denotes mouse
T9522 29261-29264 CC denotes and
T9523 29265-29271 NN denotes archea
T9521 29272-29275 NN denotes MCM
T9524 29275-29277 , denotes ,
T9525 29277-29279 IN denotes as
T9526 29280-29288 VBN denotes revealed
T9527 29289-29291 IN denotes by
T9528 29292-29300 NN denotes electron
T9529 29301-29311 NN denotes microscopy
T9530 29311-29313 , denotes ,
T9496 29313-29317 VBP denotes bear
T9531 29318-29322 JJ denotes much
T9532 29323-29333 NN denotes similarity
T9533 29334-29336 IN denotes to
T9534 29337-29340 DT denotes the
T9536 29341-29349 RB denotes recently
T9537 29350-29356 VBN denotes solved
T9535 29357-29366 NN denotes structure
T9538 29367-29369 IN denotes of
T9539 29370-29373 DT denotes the
T9541 29374-29378 NN denotes SV40
T9542 29379-29384 JJ denotes large
T9543 29385-29386 NN denotes T
T9544 29386-29387 HYPH denotes -
T9540 29387-29394 NN denotes antigen
T9545 29395-29396 -LRB- denotes (
T9547 29396-29397 CD denotes 4
T9548 29397-29398 , denotes ,
T9546 29398-29399 CD denotes 6
T9549 29399-29400 -RRB- denotes )
T9550 29400-29402 , denotes ,
T9551 29402-29405 CC denotes and
T9552 29406-29409 VBP denotes are
T9553 29410-29412 IN denotes in
T9554 29413-29418 NN denotes favor
T9555 29419-29421 IN denotes of
T9556 29422-29425 DT denotes the
T9558 29426-29432 JJ denotes double
T9560 29432-29433 HYPH denotes -
T9559 29433-29442 JJ denotes hexameric
T9557 29443-29452 NN denotes structure
T9561 29453-29455 IN denotes of
T9562 29456-29461 NN denotes mouse
T9563 29462-29465 NN denotes Mcm
T9564 29466-29468 IN denotes on
T9565 29469-29470 DT denotes a
T9567 29471-29477 NN denotes bubble
T9566 29478-29481 NN denotes DNA
T9568 29481-29482 . denotes .
T9856 29484-29493 NN denotes Substrate
T9858 29494-29497 CC denotes and
T9859 29498-29506 NN denotes sequence
T9857 29507-29518 NN denotes requirement
T9860 29519-29522 IN denotes for
T9861 29523-29527 NN denotes Mcm4
T9863 29527-29528 HYPH denotes /
T9864 29528-29529 CD denotes 6
T9865 29529-29530 HYPH denotes /
T9866 29530-29531 CD denotes 7
T9867 29532-29540 NN denotes helicase
T9862 29541-29551 NN denotes activation
T9868 29551-29729 sentence denotes DNA binding assays indicate that Mcm4/6/7 binds to those substrates containing single-stranded DNA regions regardless of the presence or absence of single-stranded 3′ or 5′ end.
T9869 29552-29555 NN denotes DNA
T9870 29556-29563 NN denotes binding
T9871 29564-29570 NNS denotes assays
T9872 29571-29579 VBP denotes indicate
T9873 29580-29584 IN denotes that
T9875 29585-29589 NN denotes Mcm4
T9876 29589-29590 HYPH denotes /
T9877 29590-29591 CD denotes 6
T9878 29591-29592 HYPH denotes /
T9879 29592-29593 CD denotes 7
T9874 29594-29599 VBZ denotes binds
T9880 29600-29602 IN denotes to
T9881 29603-29608 DT denotes those
T9882 29609-29619 NNS denotes substrates
T9883 29620-29630 VBG denotes containing
T9884 29631-29637 JJ denotes single
T9886 29637-29638 HYPH denotes -
T9885 29638-29646 VBN denotes stranded
T9888 29647-29650 NN denotes DNA
T9887 29651-29658 NNS denotes regions
T9889 29659-29669 RB denotes regardless
T9890 29670-29672 IN denotes of
T9891 29673-29676 DT denotes the
T9892 29677-29685 NN denotes presence
T9893 29686-29688 CC denotes or
T9894 29689-29696 NN denotes absence
T9895 29697-29699 IN denotes of
T9896 29700-29706 JJ denotes single
T9898 29706-29707 HYPH denotes -
T9897 29707-29715 VBN denotes stranded
T9900 29716-29717 CD denotes 3
T9901 29717-29718 SYM denotes ′
T9902 29719-29721 CC denotes or
T9903 29722-29723 CD denotes 5
T9904 29723-29724 SYM denotes ′
T9899 29725-29728 NN denotes end
T9905 29728-29729 . denotes .
T9906 29729-29830 sentence denotes Unwinding of the duplex DNA depends on translocation of single-stranded DNA from 3′ to 5′ direction.
T9907 29730-29739 NN denotes Unwinding
T9909 29740-29742 IN denotes of
T9910 29743-29746 DT denotes the
T9912 29747-29753 NN denotes duplex
T9911 29754-29757 NN denotes DNA
T9908 29758-29765 VBZ denotes depends
T9913 29766-29768 IN denotes on
T9914 29769-29782 NN denotes translocation
T9915 29783-29785 IN denotes of
T9916 29786-29792 JJ denotes single
T9918 29792-29793 HYPH denotes -
T9917 29793-29801 VBN denotes stranded
T9919 29802-29805 NN denotes DNA
T9920 29806-29810 IN denotes from
T9921 29811-29812 CD denotes 3
T9922 29812-29813 SYM denotes ′
T9923 29814-29816 IN denotes to
T9924 29817-29818 CD denotes 5
T9925 29818-29819 SYM denotes ′
T9926 29820-29829 NN denotes direction
T9927 29829-29830 . denotes .
T9928 29830-29966 sentence denotes This is most clearly shown by its helicase action on A-fork[5′] and 3′-extension but not on A-fork[3′], A-fork[3′,5′] nor 5′-extension.
T9929 29831-29835 DT denotes This
T9931 29836-29838 VBZ denotes is
T9932 29839-29843 RBS denotes most
T9933 29844-29851 RB denotes clearly
T9930 29852-29857 VBN denotes shown
T9934 29858-29860 IN denotes by
T9935 29861-29864 PRP$ denotes its
T9937 29865-29873 NN denotes helicase
T9936 29874-29880 NN denotes action
T9938 29881-29883 IN denotes on
T9939 29884-29885 NN denotes A
T9941 29885-29886 HYPH denotes -
T9940 29886-29890 NN denotes fork
T9943 29890-29891 -LRB- denotes [
T9944 29891-29892 CD denotes 5
T9945 29892-29893 SYM denotes ′
T9946 29893-29894 -RRB- denotes ]
T9947 29895-29898 CC denotes and
T9948 29899-29900 CD denotes 3
T9949 29900-29901 SYM denotes ′
T9950 29901-29902 HYPH denotes -
T9942 29902-29911 NN denotes extension
T9951 29912-29915 CC denotes but
T9952 29916-29919 RB denotes not
T9953 29920-29922 IN denotes on
T9954 29923-29924 NN denotes A
T9956 29924-29925 HYPH denotes -
T9955 29925-29929 NN denotes fork
T9958 29929-29930 -LRB- denotes [
T9959 29930-29931 CD denotes 3
T9960 29931-29932 SYM denotes ′
T9961 29932-29933 -RRB- denotes ]
T9962 29933-29935 , denotes ,
T9963 29935-29936 NN denotes A
T9965 29936-29937 HYPH denotes -
T9964 29937-29941 NN denotes fork
T9966 29941-29942 -LRB- denotes [
T9967 29942-29943 CD denotes 3
T9968 29943-29944 SYM denotes ′
T9969 29944-29945 , denotes ,
T9970 29945-29946 CD denotes 5
T9971 29946-29947 SYM denotes ′
T9972 29947-29948 -RRB- denotes ]
T9973 29949-29952 CC denotes nor
T9974 29953-29954 CD denotes 5
T9975 29954-29955 SYM denotes ′
T9976 29955-29956 HYPH denotes -
T9957 29956-29965 NN denotes extension
T9977 29965-29966 . denotes .
T9978 29966-30127 sentence denotes However, it does not necessarily require 3′ end of single-stranded DNA, since Mcm4/6/7 can displace annealing oligonucleotide on a circular single-stranded DNA.
T9979 29967-29974 RB denotes However
T9981 29974-29976 , denotes ,
T9982 29976-29978 PRP denotes it
T9983 29979-29983 VBZ denotes does
T9984 29984-29987 RB denotes not
T9985 29988-29999 RB denotes necessarily
T9980 30000-30007 VB denotes require
T9986 30008-30009 CD denotes 3
T9988 30009-30010 SYM denotes ′
T9987 30011-30014 NN denotes end
T9989 30015-30017 IN denotes of
T9990 30018-30024 JJ denotes single
T9992 30024-30025 HYPH denotes -
T9991 30025-30033 VBN denotes stranded
T9993 30034-30037 NN denotes DNA
T9994 30037-30039 , denotes ,
T9995 30039-30044 IN denotes since
T9997 30045-30049 NN denotes Mcm4
T9998 30049-30050 HYPH denotes /
T9999 30050-30051 CD denotes 6
T10000 30051-30052 HYPH denotes /
T10001 30052-30053 CD denotes 7
T10002 30054-30057 MD denotes can
T9996 30058-30066 VB denotes displace
T10003 30067-30076 VBG denotes annealing
T10004 30077-30092 NN denotes oligonucleotide
T10005 30093-30095 IN denotes on
T10006 30096-30097 DT denotes a
T10008 30098-30106 JJ denotes circular
T10009 30107-30113 JJ denotes single
T10011 30113-30114 HYPH denotes -
T10010 30114-30122 VBN denotes stranded
T10007 30123-30126 NN denotes DNA
T10012 30126-30127 . denotes .
T10013 30127-30285 sentence denotes The ability of the mouse Mcm4/6/7 to unwind 3′-extension is shared by the archaeal Mcm helicase but not by Mcm4/6/7 from S.pombe and S.cerevisiae (14,26,27).
T10014 30128-30131 DT denotes The
T10015 30132-30139 NN denotes ability
T10017 30140-30142 IN denotes of
T10018 30143-30146 DT denotes the
T10020 30147-30152 NN denotes mouse
T10019 30153-30157 NN denotes Mcm4
T10021 30157-30158 HYPH denotes /
T10022 30158-30159 CD denotes 6
T10023 30159-30160 HYPH denotes /
T10024 30160-30161 CD denotes 7
T10025 30162-30164 TO denotes to
T10026 30165-30171 VB denotes unwind
T10027 30172-30173 CD denotes 3
T10029 30173-30174 SYM denotes ′
T10030 30174-30175 HYPH denotes -
T10028 30175-30184 NN denotes extension
T10031 30185-30187 VBZ denotes is
T10016 30188-30194 VBN denotes shared
T10032 30195-30197 IN denotes by
T10033 30198-30201 DT denotes the
T10035 30202-30210 JJ denotes archaeal
T10036 30211-30214 NN denotes Mcm
T10034 30215-30223 NN denotes helicase
T10037 30224-30227 CC denotes but
T10038 30228-30231 RB denotes not
T10039 30232-30234 IN denotes by
T10040 30235-30239 NN denotes Mcm4
T10041 30239-30240 HYPH denotes /
T10042 30240-30241 CD denotes 6
T10043 30241-30242 HYPH denotes /
T10044 30242-30243 CD denotes 7
T10045 30244-30248 IN denotes from
T10046 30249-30256 NNP denotes S.pombe
T10047 30257-30260 CC denotes and
T10048 30261-30273 NNP denotes S.cerevisiae
T10049 30274-30275 -LRB- denotes (
T10051 30275-30277 CD denotes 14
T10052 30277-30278 , denotes ,
T10053 30278-30280 CD denotes 26
T10054 30280-30281 , denotes ,
T10050 30281-30283 CD denotes 27
T10055 30283-30284 -RRB- denotes )
T10056 30284-30285 . denotes .
T10057 30285-30434 sentence denotes The archaeal Mcm can unwind A-fork[3′] but eukaryotic Mcm4/6/7 cannot, since the former binds to double-stranded DNA but the latter does not (5,27).
T10058 30286-30289 DT denotes The
T10060 30290-30298 JJ denotes archaeal
T10059 30299-30302 NN denotes Mcm
T10062 30303-30306 MD denotes can
T10061 30307-30313 VB denotes unwind
T10063 30314-30315 NN denotes A
T10065 30315-30316 HYPH denotes -
T10064 30316-30320 NN denotes fork
T10066 30320-30321 -LRB- denotes [
T10067 30321-30322 CD denotes 3
T10068 30322-30323 SYM denotes ′
T10069 30323-30324 -RRB- denotes ]
T10070 30325-30328 CC denotes but
T10071 30329-30339 JJ denotes eukaryotic
T10072 30340-30344 NN denotes Mcm4
T10074 30344-30345 HYPH denotes /
T10075 30345-30346 CD denotes 6
T10076 30346-30347 HYPH denotes /
T10077 30347-30348 CD denotes 7
T10073 30349-30352 MD denotes can
T10078 30352-30355 RB denotes not
T10079 30355-30357 , denotes ,
T10080 30357-30362 IN denotes since
T10082 30363-30366 DT denotes the
T10083 30367-30373 NN denotes former
T10081 30374-30379 VBZ denotes binds
T10084 30380-30382 IN denotes to
T10085 30383-30389 JJ denotes double
T10087 30389-30390 HYPH denotes -
T10086 30390-30398 VBN denotes stranded
T10088 30399-30402 NN denotes DNA
T10089 30403-30406 CC denotes but
T10090 30407-30410 DT denotes the
T10091 30411-30417 JJ denotes latter
T10093 30418-30422 VBZ denotes does
T10094 30423-30426 RB denotes not
T10095 30427-30428 -LRB- denotes (
T10096 30428-30429 CD denotes 5
T10097 30429-30430 , denotes ,
T10092 30430-30432 CD denotes 27
T10098 30432-30433 -RRB- denotes )
T10099 30433-30434 . denotes .
T10100 30434-30564 sentence denotes While yeast Mcm helicases can translocate on duplex DNA, such activity was not observed with mammalian Mcm4/6/7 (data not shown).
T10101 30435-30440 IN denotes While
T10103 30441-30446 NN denotes yeast
T10104 30447-30450 NN denotes Mcm
T10105 30451-30460 NNS denotes helicases
T10106 30461-30464 MD denotes can
T10102 30465-30476 VB denotes translocate
T10108 30477-30479 IN denotes on
T10109 30480-30486 NN denotes duplex
T10110 30487-30490 NN denotes DNA
T10111 30490-30492 , denotes ,
T10112 30492-30496 JJ denotes such
T10113 30497-30505 NN denotes activity
T10114 30506-30509 VBD denotes was
T10115 30510-30513 RB denotes not
T10107 30514-30522 VBN denotes observed
T10116 30523-30527 IN denotes with
T10117 30528-30537 JJ denotes mammalian
T10118 30538-30542 NN denotes Mcm4
T10119 30542-30543 HYPH denotes /
T10120 30543-30544 CD denotes 6
T10121 30544-30545 HYPH denotes /
T10122 30545-30546 CD denotes 7
T10123 30547-30548 -LRB- denotes (
T10125 30548-30552 NNS denotes data
T10126 30553-30556 RB denotes not
T10124 30557-30562 VBN denotes shown
T10127 30562-30563 -RRB- denotes )
T10128 30563-30564 . denotes .
T10129 30564-30901 sentence denotes Occurrence of AT-rich sequences, with asymmetric distribution of adenine and thymine, near the replication origins, lead us to propose that Mcm may play a role in selection of initiation sites of mammalian DNA replication, and prompted us to examine the ability of sequences from human replication origins to activate Mcm4/6/7 helicase.
T10130 30565-30575 NN denotes Occurrence
T10132 30576-30578 IN denotes of
T10133 30579-30581 NN denotes AT
T10135 30581-30582 HYPH denotes -
T10134 30582-30586 JJ denotes rich
T10136 30587-30596 NNS denotes sequences
T10137 30596-30598 , denotes ,
T10138 30598-30602 IN denotes with
T10139 30603-30613 JJ denotes asymmetric
T10140 30614-30626 NN denotes distribution
T10141 30627-30629 IN denotes of
T10142 30630-30637 NN denotes adenine
T10143 30638-30641 CC denotes and
T10144 30642-30649 NN denotes thymine
T10145 30649-30651 , denotes ,
T10146 30651-30655 IN denotes near
T10147 30656-30659 DT denotes the
T10149 30660-30671 NN denotes replication
T10148 30672-30679 NNS denotes origins
T10150 30679-30681 , denotes ,
T10131 30681-30685 VBP denotes lead
T10151 30686-30688 PRP denotes us
T10152 30689-30691 TO denotes to
T10153 30692-30699 VB denotes propose
T10154 30700-30704 IN denotes that
T10156 30705-30708 NNP denotes Mcm
T10157 30709-30712 MD denotes may
T10155 30713-30717 VB denotes play
T10158 30718-30719 DT denotes a
T10159 30720-30724 NN denotes role
T10160 30725-30727 IN denotes in
T10161 30728-30737 NN denotes selection
T10162 30738-30740 IN denotes of
T10163 30741-30751 NN denotes initiation
T10164 30752-30757 NNS denotes sites
T10165 30758-30760 IN denotes of
T10166 30761-30770 JJ denotes mammalian
T10167 30771-30774 NN denotes DNA
T10168 30775-30786 NN denotes replication
T10169 30786-30788 , denotes ,
T10170 30788-30791 CC denotes and
T10171 30792-30800 VBD denotes prompted
T10172 30801-30803 PRP denotes us
T10173 30804-30806 TO denotes to
T10174 30807-30814 VB denotes examine
T10175 30815-30818 DT denotes the
T10176 30819-30826 NN denotes ability
T10177 30827-30829 IN denotes of
T10178 30830-30839 NNS denotes sequences
T10179 30840-30844 IN denotes from
T10180 30845-30850 JJ denotes human
T10182 30851-30862 NN denotes replication
T10181 30863-30870 NNS denotes origins
T10183 30871-30873 TO denotes to
T10184 30874-30882 VB denotes activate
T10185 30883-30887 NN denotes Mcm4
T10187 30887-30888 HYPH denotes /
T10188 30888-30889 CD denotes 6
T10189 30889-30890 HYPH denotes /
T10190 30890-30891 CD denotes 7
T10186 30892-30900 NN denotes helicase
T10191 30900-30901 . denotes .
T10192 30901-30990 sentence denotes Both lamin-B2 and c-myc origins served as efficient activator for Mcm helicase in vitro.
T10193 30902-30906 CC denotes Both
T10195 30907-30912 NN denotes lamin
T10196 30912-30913 HYPH denotes -
T10194 30913-30915 NN denotes B2
T10198 30916-30919 CC denotes and
T10199 30920-30921 NN denotes c
T10201 30921-30922 HYPH denotes -
T10200 30922-30925 NN denotes myc
T10197 30926-30933 NNS denotes origins
T10202 30934-30940 VBD denotes served
T10203 30941-30943 IN denotes as
T10204 30944-30953 JJ denotes efficient
T10205 30954-30963 NN denotes activator
T10206 30964-30967 IN denotes for
T10207 30968-30971 NN denotes Mcm
T10208 30972-30980 NN denotes helicase
T10209 30981-30983 FW denotes in
T10210 30984-30989 FW denotes vitro
T10211 30989-30990 . denotes .
T10212 30990-31119 sentence denotes Consistent with it, site-specific loading of Mcm in the DNA replication initiation zone of the c-myc was recently reported (33).
T10213 30991-31001 JJ denotes Consistent
T10215 31002-31006 IN denotes with
T10216 31007-31009 PRP denotes it
T10217 31009-31011 , denotes ,
T10218 31011-31015 NN denotes site
T10220 31015-31016 HYPH denotes -
T10219 31016-31024 JJ denotes specific
T10221 31025-31032 NN denotes loading
T10222 31033-31035 IN denotes of
T10223 31036-31039 NN denotes Mcm
T10224 31040-31042 IN denotes in
T10225 31043-31046 DT denotes the
T10227 31047-31050 NN denotes DNA
T10229 31051-31062 NN denotes replication
T10228 31063-31073 NN denotes initiation
T10226 31074-31078 NN denotes zone
T10230 31079-31081 IN denotes of
T10231 31082-31085 DT denotes the
T10233 31086-31087 NN denotes c
T10234 31087-31088 HYPH denotes -
T10232 31088-31091 NN denotes myc
T10235 31092-31095 VBD denotes was
T10236 31096-31104 RB denotes recently
T10214 31105-31113 VBN denotes reported
T10237 31114-31115 -LRB- denotes (
T10238 31115-31117 CD denotes 33
T10239 31117-31118 -RRB- denotes )
T10240 31118-31119 . denotes .
T10241 31119-31237 sentence denotes We have examined in detail the effect of sequence context of the single-stranded DNA on the helicase activity of Mcm.
T10242 31120-31122 PRP denotes We
T10244 31123-31127 VBP denotes have
T10243 31128-31136 VBN denotes examined
T10245 31137-31139 IN denotes in
T10246 31140-31146 NN denotes detail
T10247 31147-31150 DT denotes the
T10248 31151-31157 NN denotes effect
T10249 31158-31160 IN denotes of
T10250 31161-31169 NN denotes sequence
T10251 31170-31177 NN denotes context
T10252 31178-31180 IN denotes of
T10253 31181-31184 DT denotes the
T10255 31185-31191 JJ denotes single
T10257 31191-31192 HYPH denotes -
T10256 31192-31200 VBN denotes stranded
T10254 31201-31204 NN denotes DNA
T10258 31205-31207 IN denotes on
T10259 31208-31211 DT denotes the
T10261 31212-31220 NN denotes helicase
T10260 31221-31229 NN denotes activity
T10262 31230-31232 IN denotes of
T10263 31233-31236 NN denotes Mcm
T10264 31236-31237 . denotes .
T10265 31237-31335 sentence denotes The results indicate that thymine content of 50% is sufficient for the maximum helicase activity.
T10266 31238-31241 DT denotes The
T10267 31242-31249 NNS denotes results
T10268 31250-31258 VBP denotes indicate
T10269 31259-31263 IN denotes that
T10271 31264-31271 NN denotes thymine
T10272 31272-31279 NN denotes content
T10273 31280-31282 IN denotes of
T10274 31283-31285 CD denotes 50
T10275 31285-31286 NN denotes %
T10270 31287-31289 VBZ denotes is
T10276 31290-31300 JJ denotes sufficient
T10277 31301-31304 IN denotes for
T10278 31305-31308 DT denotes the
T10280 31309-31316 JJ denotes maximum
T10281 31317-31325 NN denotes helicase
T10279 31326-31334 NN denotes activity
T10282 31334-31335 . denotes .
T10283 31335-31441 sentence denotes The efficiency of displacement decreased as the thymine content of the 3′-tail dropped to 33% (Figure 6).
T10284 31336-31339 DT denotes The
T10285 31340-31350 NN denotes efficiency
T10287 31351-31353 IN denotes of
T10288 31354-31366 NN denotes displacement
T10286 31367-31376 VBD denotes decreased
T10289 31377-31379 IN denotes as
T10291 31380-31383 DT denotes the
T10293 31384-31391 NN denotes thymine
T10292 31392-31399 NN denotes content
T10294 31400-31402 IN denotes of
T10295 31403-31406 DT denotes the
T10297 31407-31408 CD denotes 3
T10298 31408-31409 SYM denotes ′
T10299 31409-31410 HYPH denotes -
T10296 31410-31414 NN denotes tail
T10290 31415-31422 VBD denotes dropped
T10300 31423-31425 IN denotes to
T10301 31426-31428 CD denotes 33
T10302 31428-31429 NN denotes %
T10303 31430-31431 -LRB- denotes (
T10304 31431-31437 NN denotes Figure
T10305 31438-31439 CD denotes 6
T10306 31439-31440 -RRB- denotes )
T10307 31440-31441 . denotes .
T10308 31441-31585 sentence denotes The stretches of thymine residues may not be necessarily required, since repeats of TC dinucleotides served as a potent activator for Mcm4/6/7.
T10309 31442-31445 DT denotes The
T10310 31446-31455 NNS denotes stretches
T10312 31456-31458 IN denotes of
T10313 31459-31466 NN denotes thymine
T10314 31467-31475 NNS denotes residues
T10315 31476-31479 MD denotes may
T10316 31480-31483 RB denotes not
T10317 31484-31486 VB denotes be
T10318 31487-31498 RB denotes necessarily
T10311 31499-31507 VBN denotes required
T10319 31507-31509 , denotes ,
T10320 31509-31514 IN denotes since
T10322 31515-31522 NNS denotes repeats
T10323 31523-31525 IN denotes of
T10324 31526-31528 NN denotes TC
T10325 31529-31542 NNS denotes dinucleotides
T10321 31543-31549 VBD denotes served
T10326 31550-31552 IN denotes as
T10327 31553-31554 DT denotes a
T10329 31555-31561 JJ denotes potent
T10328 31562-31571 NN denotes activator
T10330 31572-31575 IN denotes for
T10331 31576-31580 NN denotes Mcm4
T10332 31580-31581 HYPH denotes /
T10333 31581-31582 CD denotes 6
T10334 31582-31583 HYPH denotes /
T10335 31583-31584 CD denotes 7
T10336 31584-31585 . denotes .
T10337 31585-31710 sentence denotes We also noticed that the presence of a secondary structure within the single-stranded DNA is inhibitory for helicase action.
T10338 31586-31588 PRP denotes We
T10340 31589-31593 RB denotes also
T10339 31594-31601 VBD denotes noticed
T10341 31602-31606 IN denotes that
T10343 31607-31610 DT denotes the
T10344 31611-31619 NN denotes presence
T10345 31620-31622 IN denotes of
T10346 31623-31624 DT denotes a
T10348 31625-31634 JJ denotes secondary
T10347 31635-31644 NN denotes structure
T10349 31645-31651 IN denotes within
T10350 31652-31655 DT denotes the
T10352 31656-31662 JJ denotes single
T10354 31662-31663 HYPH denotes -
T10353 31663-31671 VBN denotes stranded
T10351 31672-31675 NN denotes DNA
T10342 31676-31678 VBZ denotes is
T10355 31679-31689 JJ denotes inhibitory
T10356 31690-31693 IN denotes for
T10357 31694-31702 NN denotes helicase
T10358 31703-31709 NN denotes action
T10359 31709-31710 . denotes .
T10360 31710-31822 sentence denotes Nuclease footprinting assays indicated that binding was interfered by the secondary structure (data not shown).
T10361 31711-31719 NN denotes Nuclease
T10362 31720-31732 NN denotes footprinting
T10363 31733-31739 NNS denotes assays
T10364 31740-31749 VBD denotes indicated
T10365 31750-31754 IN denotes that
T10367 31755-31762 NN denotes binding
T10368 31763-31766 VBD denotes was
T10366 31767-31777 VBN denotes interfered
T10369 31778-31780 IN denotes by
T10370 31781-31784 DT denotes the
T10372 31785-31794 JJ denotes secondary
T10371 31795-31804 NN denotes structure
T10373 31805-31806 -LRB- denotes (
T10375 31806-31810 NNS denotes data
T10376 31811-31814 RB denotes not
T10374 31815-31820 VBN denotes shown
T10377 31820-31821 -RRB- denotes )
T10378 31821-31822 . denotes .
T10379 31822-32230 sentence denotes Thus, we have concluded that Mcm4/6/7 helicase is most efficiently activated by non-structured single-stranded DNA with thymine content of 50% or more, although significant stimulation is observed also by DNA with less thymine content (Figure 7), suggesting that the sequence specificity for Mcm helicase activation is rather relaxed and that the extent of the activation may depend on the sequence context.
T10380 31823-31827 RB denotes Thus
T10382 31827-31829 , denotes ,
T10383 31829-31831 PRP denotes we
T10384 31832-31836 VBP denotes have
T10381 31837-31846 VBN denotes concluded
T10385 31847-31851 IN denotes that
T10387 31852-31856 NN denotes Mcm4
T10389 31856-31857 HYPH denotes /
T10390 31857-31858 CD denotes 6
T10391 31858-31859 HYPH denotes /
T10392 31859-31860 CD denotes 7
T10388 31861-31869 NN denotes helicase
T10393 31870-31872 VBZ denotes is
T10394 31873-31877 RBS denotes most
T10395 31878-31889 RB denotes efficiently
T10386 31890-31899 VBN denotes activated
T10396 31900-31902 IN denotes by
T10397 31903-31917 JJ denotes non-structured
T10399 31918-31924 JJ denotes single
T10401 31924-31925 HYPH denotes -
T10400 31925-31933 VBN denotes stranded
T10398 31934-31937 NN denotes DNA
T10402 31938-31942 IN denotes with
T10403 31943-31950 NN denotes thymine
T10404 31951-31958 NN denotes content
T10405 31959-31961 IN denotes of
T10406 31962-31964 CD denotes 50
T10407 31964-31965 NN denotes %
T10408 31966-31968 CC denotes or
T10409 31969-31973 JJR denotes more
T10410 31973-31975 , denotes ,
T10411 31975-31983 IN denotes although
T10413 31984-31995 JJ denotes significant
T10414 31996-32007 NN denotes stimulation
T10415 32008-32010 VBZ denotes is
T10412 32011-32019 VBN denotes observed
T10416 32020-32024 RB denotes also
T10417 32025-32027 IN denotes by
T10418 32028-32031 NN denotes DNA
T10419 32032-32036 IN denotes with
T10420 32037-32041 JJR denotes less
T10422 32042-32049 NN denotes thymine
T10421 32050-32057 NN denotes content
T10423 32058-32059 -LRB- denotes (
T10424 32059-32065 NN denotes Figure
T10425 32066-32067 CD denotes 7
T10426 32067-32068 -RRB- denotes )
T10427 32068-32070 , denotes ,
T10428 32070-32080 VBG denotes suggesting
T10429 32081-32085 IN denotes that
T10431 32086-32089 DT denotes the
T10433 32090-32098 NN denotes sequence
T10432 32099-32110 NN denotes specificity
T10434 32111-32114 IN denotes for
T10435 32115-32118 NN denotes Mcm
T10436 32119-32127 NN denotes helicase
T10437 32128-32138 NN denotes activation
T10430 32139-32141 VBZ denotes is
T10438 32142-32148 RB denotes rather
T10439 32149-32156 JJ denotes relaxed
T10440 32157-32160 CC denotes and
T10441 32161-32165 IN denotes that
T10443 32166-32169 DT denotes the
T10444 32170-32176 NN denotes extent
T10445 32177-32179 IN denotes of
T10446 32180-32183 DT denotes the
T10447 32184-32194 NN denotes activation
T10448 32195-32198 MD denotes may
T10442 32199-32205 VB denotes depend
T10449 32206-32208 IN denotes on
T10450 32209-32212 DT denotes the
T10452 32213-32221 NN denotes sequence
T10451 32222-32229 NN denotes context
T10453 32229-32230 . denotes .
T10454 32230-32528 sentence denotes This would be reasonable given the flexibility and differential regulation of site selection for initiation of DNA replication during development or in various cell types, as well as the variability in initiation potential of each replication origin on the genome even within the single cell type.
T10455 32231-32235 DT denotes This
T10457 32236-32241 MD denotes would
T10456 32242-32244 VB denotes be
T10458 32245-32255 JJ denotes reasonable
T10459 32256-32261 VBN denotes given
T10460 32262-32265 DT denotes the
T10461 32266-32277 NN denotes flexibility
T10462 32278-32281 CC denotes and
T10463 32282-32294 JJ denotes differential
T10464 32295-32305 NN denotes regulation
T10465 32306-32308 IN denotes of
T10466 32309-32313 NN denotes site
T10467 32314-32323 NN denotes selection
T10468 32324-32327 IN denotes for
T10469 32328-32338 NN denotes initiation
T10470 32339-32341 IN denotes of
T10471 32342-32345 NN denotes DNA
T10472 32346-32357 NN denotes replication
T10473 32358-32364 IN denotes during
T10474 32365-32376 NN denotes development
T10475 32377-32379 CC denotes or
T10476 32380-32382 IN denotes in
T10477 32383-32390 JJ denotes various
T10479 32391-32395 NN denotes cell
T10478 32396-32401 NNS denotes types
T10480 32401-32403 , denotes ,
T10481 32403-32405 RB denotes as
T10483 32406-32410 RB denotes well
T10482 32411-32413 IN denotes as
T10484 32414-32417 DT denotes the
T10485 32418-32429 NN denotes variability
T10486 32430-32432 IN denotes in
T10487 32433-32443 NN denotes initiation
T10488 32444-32453 NN denotes potential
T10489 32454-32456 IN denotes of
T10490 32457-32461 DT denotes each
T10492 32462-32473 NN denotes replication
T10491 32474-32480 NN denotes origin
T10493 32481-32483 IN denotes on
T10494 32484-32487 DT denotes the
T10495 32488-32494 NN denotes genome
T10496 32495-32499 RB denotes even
T10497 32500-32506 IN denotes within
T10498 32507-32510 DT denotes the
T10500 32511-32517 JJ denotes single
T10501 32518-32522 NN denotes cell
T10499 32523-32527 NN denotes type
T10502 32527-32528 . denotes .
T10855 32530-32534 NN denotes Mcm4
T10857 32534-32535 HYPH denotes /
T10858 32535-32536 CD denotes 6
T10859 32536-32537 HYPH denotes /
T10860 32537-32538 CD denotes 7
T10856 32539-32547 NN denotes helicase
T10861 32548-32554 IN denotes during
T10862 32555-32565 JJ denotes processive
T10863 32566-32575 NN denotes unwinding
T10864 32576-32578 IN denotes at
T10865 32579-32582 DT denotes the
T10866 32583-32587 NN denotes fork
T10867 32587-32773 sentence denotes The specific requirement of single-stranded thymine residues for activation of Mcm helicase prompted us to examine whether they are required also for processive unwinding of duplex DNA.
T10868 32588-32591 DT denotes The
T10870 32592-32600 JJ denotes specific
T10869 32601-32612 NN denotes requirement
T10872 32613-32615 IN denotes of
T10873 32616-32622 JJ denotes single
T10875 32622-32623 HYPH denotes -
T10874 32623-32631 VBN denotes stranded
T10877 32632-32639 NN denotes thymine
T10876 32640-32648 NNS denotes residues
T10878 32649-32652 IN denotes for
T10879 32653-32663 NN denotes activation
T10880 32664-32666 IN denotes of
T10881 32667-32670 NN denotes Mcm
T10882 32671-32679 NN denotes helicase
T10871 32680-32688 VBD denotes prompted
T10883 32689-32691 PRP denotes us
T10884 32692-32694 TO denotes to
T10885 32695-32702 VB denotes examine
T10886 32703-32710 IN denotes whether
T10888 32711-32715 PRP denotes they
T10889 32716-32719 VBP denotes are
T10887 32720-32728 VBN denotes required
T10890 32729-32733 RB denotes also
T10891 32734-32737 IN denotes for
T10892 32738-32748 JJ denotes processive
T10893 32749-32758 NN denotes unwinding
T10894 32759-32761 IN denotes of
T10895 32762-32768 NN denotes duplex
T10896 32769-32772 NN denotes DNA
T10897 32772-32773 . denotes .
T10898 32773-32893 sentence denotes Our results indicated that increase of GC pair in the duplex segment significantly inhibited the Mcm helicase activity.
T10899 32774-32777 PRP$ denotes Our
T10900 32778-32785 NNS denotes results
T10901 32786-32795 VBD denotes indicated
T10902 32796-32800 IN denotes that
T10904 32801-32809 NN denotes increase
T10905 32810-32812 IN denotes of
T10906 32813-32815 NN denotes GC
T10907 32816-32820 NN denotes pair
T10908 32821-32823 IN denotes in
T10909 32824-32827 DT denotes the
T10911 32828-32834 NN denotes duplex
T10910 32835-32842 NN denotes segment
T10912 32843-32856 RB denotes significantly
T10903 32857-32866 VBD denotes inhibited
T10913 32867-32870 DT denotes the
T10915 32871-32874 NN denotes Mcm
T10916 32875-32883 NN denotes helicase
T10914 32884-32892 NN denotes activity
T10917 32892-32893 . denotes .
T10918 32893-33065 sentence denotes Duplex DNA composed only of GC pairs (10 repeats of CGG) on a Y-fork was not displaced at all, while the same template was readily displaced by SV40 T-antigen (Figure 8B).
T10919 32894-32900 NN denotes Duplex
T10920 32901-32904 NN denotes DNA
T10922 32905-32913 VBN denotes composed
T10923 32914-32918 RB denotes only
T10924 32919-32921 IN denotes of
T10925 32922-32924 NN denotes GC
T10926 32925-32930 NNS denotes pairs
T10927 32931-32932 -LRB- denotes (
T10929 32932-32934 CD denotes 10
T10928 32935-32942 NNS denotes repeats
T10930 32943-32945 IN denotes of
T10931 32946-32949 NN denotes CGG
T10932 32949-32950 -RRB- denotes )
T10933 32951-32953 IN denotes on
T10934 32954-32955 DT denotes a
T10936 32956-32957 NN denotes Y
T10937 32957-32958 HYPH denotes -
T10935 32958-32962 NN denotes fork
T10938 32963-32966 VBD denotes was
T10939 32967-32970 RB denotes not
T10921 32971-32980 VBN denotes displaced
T10940 32981-32983 RB denotes at
T10941 32984-32987 RB denotes all
T10942 32987-32989 , denotes ,
T10943 32989-32994 IN denotes while
T10945 32995-32998 DT denotes the
T10947 32999-33003 JJ denotes same
T10946 33004-33012 NN denotes template
T10948 33013-33016 VBD denotes was
T10949 33017-33024 RB denotes readily
T10944 33025-33034 VBN denotes displaced
T10950 33035-33037 IN denotes by
T10951 33038-33042 NN denotes SV40
T10953 33043-33044 NN denotes T
T10954 33044-33045 HYPH denotes -
T10952 33045-33052 NN denotes antigen
T10955 33053-33054 -LRB- denotes (
T10957 33054-33060 NN denotes Figure
T10956 33061-33063 NN denotes 8B
T10958 33063-33064 -RRB- denotes )
T10959 33064-33065 . denotes .
T10960 33065-33201 sentence denotes Mcm helicase was inhibited by the presence of GC-rich duplex segment also on a circular single-stranded partial heteroduplex substrate.
T10961 33066-33069 NN denotes Mcm
T10962 33070-33078 NN denotes helicase
T10964 33079-33082 VBD denotes was
T10963 33083-33092 VBN denotes inhibited
T10965 33093-33095 IN denotes by
T10966 33096-33099 DT denotes the
T10967 33100-33108 NN denotes presence
T10968 33109-33111 IN denotes of
T10969 33112-33114 NN denotes GC
T10971 33114-33115 HYPH denotes -
T10970 33115-33119 JJ denotes rich
T10973 33120-33126 NN denotes duplex
T10972 33127-33134 NN denotes segment
T10974 33135-33139 RB denotes also
T10975 33140-33142 IN denotes on
T10976 33143-33144 DT denotes a
T10978 33145-33153 JJ denotes circular
T10979 33154-33160 JJ denotes single
T10981 33160-33161 HYPH denotes -
T10980 33161-33169 VBN denotes stranded
T10982 33170-33177 JJ denotes partial
T10983 33178-33190 NN denotes heteroduplex
T10977 33191-33200 NN denotes substrate
T10984 33200-33201 . denotes .
T10985 33201-33359 sentence denotes However, on this substrate, Mcm4/6/7 was able to displace DNA past the GC-rich region, albeit to a limited extent, when it was added at a high concentration.
T10986 33202-33209 RB denotes However
T10988 33209-33211 , denotes ,
T10989 33211-33213 IN denotes on
T10990 33214-33218 DT denotes this
T10991 33219-33228 NN denotes substrate
T10992 33228-33230 , denotes ,
T10993 33230-33234 NN denotes Mcm4
T10994 33234-33235 HYPH denotes /
T10995 33235-33236 CD denotes 6
T10996 33236-33237 HYPH denotes /
T10997 33237-33238 CD denotes 7
T10987 33239-33242 VBD denotes was
T10998 33243-33247 JJ denotes able
T10999 33248-33250 TO denotes to
T11000 33251-33259 VB denotes displace
T11001 33260-33263 NN denotes DNA
T11002 33264-33268 IN denotes past
T11003 33269-33272 DT denotes the
T11005 33273-33275 NN denotes GC
T11007 33275-33276 HYPH denotes -
T11006 33276-33280 JJ denotes rich
T11004 33281-33287 NN denotes region
T11008 33287-33289 , denotes ,
T11009 33289-33295 IN denotes albeit
T11010 33296-33298 IN denotes to
T11011 33299-33300 DT denotes a
T11013 33301-33308 VBN denotes limited
T11012 33309-33315 NN denotes extent
T11014 33315-33317 , denotes ,
T11015 33317-33321 WRB denotes when
T11017 33322-33324 PRP denotes it
T11018 33325-33328 VBD denotes was
T11016 33329-33334 VBN denotes added
T11019 33335-33337 IN denotes at
T11020 33338-33339 DT denotes a
T11022 33340-33344 JJ denotes high
T11021 33345-33358 NN denotes concentration
T11023 33358-33359 . denotes .
T11024 33359-33526 sentence denotes On the partial heteroduplex template, Mcm is loaded onto the circular single-stranded DNA of 6.4 kb, while it is loaded onto the 50 nt long 3′-tail DNA on the Y-fork.
T11025 33360-33362 IN denotes On
T11027 33363-33366 DT denotes the
T11029 33367-33374 JJ denotes partial
T11030 33375-33387 NN denotes heteroduplex
T11028 33388-33396 NN denotes template
T11031 33396-33398 , denotes ,
T11032 33398-33401 NN denotes Mcm
T11033 33402-33404 VBZ denotes is
T11026 33405-33411 VBN denotes loaded
T11034 33412-33416 IN denotes onto
T11035 33417-33420 DT denotes the
T11037 33421-33429 JJ denotes circular
T11038 33430-33436 JJ denotes single
T11040 33436-33437 HYPH denotes -
T11039 33437-33445 VBN denotes stranded
T11036 33446-33449 NN denotes DNA
T11041 33450-33452 IN denotes of
T11042 33453-33456 CD denotes 6.4
T11043 33457-33459 NN denotes kb
T11044 33459-33461 , denotes ,
T11045 33461-33466 IN denotes while
T11047 33467-33469 PRP denotes it
T11048 33470-33472 VBZ denotes is
T11046 33473-33479 VBN denotes loaded
T11049 33480-33484 IN denotes onto
T11050 33485-33488 DT denotes the
T11052 33489-33491 CD denotes 50
T11053 33492-33494 NN denotes nt
T11054 33495-33499 JJ denotes long
T11055 33500-33501 CD denotes 3
T11057 33501-33502 SYM denotes ′
T11058 33502-33503 HYPH denotes -
T11056 33503-33507 NN denotes tail
T11051 33508-33511 NN denotes DNA
T11059 33512-33514 IN denotes on
T11060 33515-33518 DT denotes the
T11062 33519-33520 NN denotes Y
T11063 33520-33521 HYPH denotes -
T11061 33521-33525 NN denotes fork
T11064 33525-33526 . denotes .
T11065 33526-33610 sentence denotes Thus, the difference of helicase actions may reflect the efficiency of Mcm loading.
T11066 33527-33531 RB denotes Thus
T11068 33531-33533 , denotes ,
T11069 33533-33536 DT denotes the
T11070 33537-33547 NN denotes difference
T11071 33548-33550 IN denotes of
T11072 33551-33559 NN denotes helicase
T11073 33560-33567 NNS denotes actions
T11074 33568-33571 MD denotes may
T11067 33572-33579 VB denotes reflect
T11075 33580-33583 DT denotes the
T11076 33584-33594 NN denotes efficiency
T11077 33595-33597 IN denotes of
T11078 33598-33601 NN denotes Mcm
T11079 33602-33609 NN denotes loading
T11080 33609-33610 . denotes .
T11081 33610-33827 sentence denotes Alternatively, the presence of ‘random’ sequence at the initially unwound duplex segment in the former template may engage the Mcm helicase in a more active conformation which can displace the GC-rich duplex segment.
T11082 33611-33624 RB denotes Alternatively
T11084 33624-33626 , denotes ,
T11085 33626-33629 DT denotes the
T11086 33630-33638 NN denotes presence
T11087 33639-33641 IN denotes of
T11088 33642-33643 `` denotes ‘
T11089 33643-33649 JJ denotes random
T11091 33649-33650 '' denotes ’
T11090 33651-33659 NN denotes sequence
T11092 33660-33662 IN denotes at
T11093 33663-33666 DT denotes the
T11095 33667-33676 RB denotes initially
T11096 33677-33684 JJ denotes unwound
T11097 33685-33691 NN denotes duplex
T11094 33692-33699 NN denotes segment
T11098 33700-33702 IN denotes in
T11099 33703-33706 DT denotes the
T11101 33707-33713 JJ denotes former
T11100 33714-33722 NN denotes template
T11102 33723-33726 MD denotes may
T11083 33727-33733 VB denotes engage
T11103 33734-33737 DT denotes the
T11105 33738-33741 NN denotes Mcm
T11104 33742-33750 NN denotes helicase
T11106 33751-33753 IN denotes in
T11107 33754-33755 DT denotes a
T11109 33756-33760 RBR denotes more
T11110 33761-33767 JJ denotes active
T11108 33768-33780 NN denotes conformation
T11111 33781-33786 WDT denotes which
T11113 33787-33790 MD denotes can
T11112 33791-33799 VB denotes displace
T11114 33800-33803 DT denotes the
T11116 33804-33806 NN denotes GC
T11118 33806-33807 HYPH denotes -
T11117 33807-33811 JJ denotes rich
T11119 33812-33818 NN denotes duplex
T11115 33819-33826 NN denotes segment
T11120 33826-33827 . denotes .
T11121 33827-33933 sentence denotes On Y-fork substrates, increase of AT base pairs in the duplex (10 repeats of CTT) restored the unwinding.
T11122 33828-33830 IN denotes On
T11124 33831-33832 NN denotes Y
T11126 33832-33833 HYPH denotes -
T11125 33833-33837 NN denotes fork
T11127 33838-33848 NNS denotes substrates
T11128 33848-33850 , denotes ,
T11129 33850-33858 NN denotes increase
T11130 33859-33861 IN denotes of
T11131 33862-33864 NN denotes AT
T11133 33865-33869 NN denotes base
T11132 33870-33875 NNS denotes pairs
T11134 33876-33878 IN denotes in
T11135 33879-33882 DT denotes the
T11136 33883-33889 NN denotes duplex
T11137 33890-33891 -LRB- denotes (
T11139 33891-33893 CD denotes 10
T11138 33894-33901 NNS denotes repeats
T11140 33902-33904 IN denotes of
T11141 33905-33908 NN denotes CTT
T11142 33908-33909 -RRB- denotes )
T11123 33910-33918 VBD denotes restored
T11143 33919-33922 DT denotes the
T11144 33923-33932 NN denotes unwinding
T11145 33932-33933 . denotes .
T11146 33933-34121 sentence denotes These results indicate either that thymine residues are required on the duplex DNA for continuous unwinding, or that Mcm4/6/7 is simply not efficient enough to unwind the stable GC pairs.
T11147 33934-33939 DT denotes These
T11148 33940-33947 NNS denotes results
T11149 33948-33956 VBP denotes indicate
T11150 33957-33963 CC denotes either
T11152 33964-33968 IN denotes that
T11153 33969-33976 NN denotes thymine
T11154 33977-33985 NNS denotes residues
T11155 33986-33989 VBP denotes are
T11151 33990-33998 VBN denotes required
T11156 33999-34001 IN denotes on
T11157 34002-34005 DT denotes the
T11159 34006-34012 NN denotes duplex
T11158 34013-34016 NN denotes DNA
T11160 34017-34020 IN denotes for
T11161 34021-34031 JJ denotes continuous
T11162 34032-34041 NN denotes unwinding
T11163 34041-34043 , denotes ,
T11164 34043-34045 CC denotes or
T11165 34046-34050 IN denotes that
T11167 34051-34055 NN denotes Mcm4
T11168 34055-34056 HYPH denotes /
T11169 34056-34057 CD denotes 6
T11170 34057-34058 HYPH denotes /
T11171 34058-34059 CD denotes 7
T11166 34060-34062 VBZ denotes is
T11172 34063-34069 RB denotes simply
T11173 34070-34073 RB denotes not
T11174 34074-34083 JJ denotes efficient
T11175 34084-34090 RB denotes enough
T11176 34091-34093 TO denotes to
T11177 34094-34100 VB denotes unwind
T11178 34101-34104 DT denotes the
T11180 34105-34111 JJ denotes stable
T11181 34112-34114 NN denotes GC
T11179 34115-34120 NNS denotes pairs
T11182 34120-34121 . denotes .
T11183 34121-34443 sentence denotes Replacement of the central guanosine with inosine in the CGG repeat duplex DNA resulted in displacement (Figure 8E) (31), suggesting that the continuous presence of AT base pair may not be essential for the Mcm helicase function and that the thermostability of GC base pairs is inhibitory for helicase action of Mcm4/6/7.
T11184 34122-34133 NN denotes Replacement
T11186 34134-34136 IN denotes of
T11187 34137-34140 DT denotes the
T11189 34141-34148 JJ denotes central
T11188 34149-34158 NN denotes guanosine
T11190 34159-34163 IN denotes with
T11191 34164-34171 NN denotes inosine
T11192 34172-34174 IN denotes in
T11193 34175-34178 DT denotes the
T11195 34179-34182 NN denotes CGG
T11196 34183-34189 NN denotes repeat
T11197 34190-34196 NN denotes duplex
T11194 34197-34200 NN denotes DNA
T11185 34201-34209 VBD denotes resulted
T11198 34210-34212 IN denotes in
T11199 34213-34225 NN denotes displacement
T11200 34226-34227 -LRB- denotes (
T11202 34227-34233 NN denotes Figure
T11201 34234-34236 NN denotes 8E
T11203 34236-34237 -RRB- denotes )
T11204 34238-34239 -LRB- denotes (
T11205 34239-34241 CD denotes 31
T11206 34241-34242 -RRB- denotes )
T11207 34242-34244 , denotes ,
T11208 34244-34254 VBG denotes suggesting
T11209 34255-34259 IN denotes that
T11211 34260-34263 DT denotes the
T11213 34264-34274 JJ denotes continuous
T11212 34275-34283 NN denotes presence
T11214 34284-34286 IN denotes of
T11215 34287-34289 NN denotes AT
T11217 34290-34294 NN denotes base
T11216 34295-34299 NN denotes pair
T11218 34300-34303 MD denotes may
T11219 34304-34307 RB denotes not
T11210 34308-34310 VB denotes be
T11220 34311-34320 JJ denotes essential
T11221 34321-34324 IN denotes for
T11222 34325-34328 DT denotes the
T11224 34329-34332 NN denotes Mcm
T11225 34333-34341 NN denotes helicase
T11223 34342-34350 NN denotes function
T11226 34351-34354 CC denotes and
T11227 34355-34359 IN denotes that
T11229 34360-34363 DT denotes the
T11230 34364-34379 NN denotes thermostability
T11231 34380-34382 IN denotes of
T11232 34383-34385 NN denotes GC
T11234 34386-34390 NN denotes base
T11233 34391-34396 NNS denotes pairs
T11228 34397-34399 VBZ denotes is
T11235 34400-34410 JJ denotes inhibitory
T11236 34411-34414 IN denotes for
T11237 34415-34423 NN denotes helicase
T11238 34424-34430 NN denotes action
T11239 34431-34433 IN denotes of
T11240 34434-34438 NN denotes Mcm4
T11241 34438-34439 HYPH denotes /
T11242 34439-34440 CD denotes 6
T11243 34440-34441 HYPH denotes /
T11244 34441-34442 CD denotes 7
T11245 34442-34443 . denotes .
T11246 34443-34632 sentence denotes The results described in this manuscript reveal potentially important features of mammalian Mcm4/6/7 helicase, which is likely to be a key component of the eukaryotic replicative helicase.
T11247 34444-34447 DT denotes The
T11248 34448-34455 NNS denotes results
T11250 34456-34465 VBN denotes described
T11251 34466-34468 IN denotes in
T11252 34469-34473 DT denotes this
T11253 34474-34484 NN denotes manuscript
T11249 34485-34491 VBP denotes reveal
T11254 34492-34503 RB denotes potentially
T11255 34504-34513 JJ denotes important
T11256 34514-34522 NNS denotes features
T11257 34523-34525 IN denotes of
T11258 34526-34535 JJ denotes mammalian
T11260 34536-34540 NN denotes Mcm4
T11261 34540-34541 HYPH denotes /
T11262 34541-34542 CD denotes 6
T11263 34542-34543 HYPH denotes /
T11264 34543-34544 CD denotes 7
T11259 34545-34553 NN denotes helicase
T11265 34553-34555 , denotes ,
T11266 34555-34560 WDT denotes which
T11267 34561-34563 VBZ denotes is
T11268 34564-34570 JJ denotes likely
T11269 34571-34573 TO denotes to
T11270 34574-34576 VB denotes be
T11271 34577-34578 DT denotes a
T11273 34579-34582 JJ denotes key
T11272 34583-34592 NN denotes component
T11274 34593-34595 IN denotes of
T11275 34596-34599 DT denotes the
T11277 34600-34610 JJ denotes eukaryotic
T11278 34611-34622 JJ denotes replicative
T11276 34623-34631 NN denotes helicase
T11279 34631-34632 . denotes .
T11280 34632-34896 sentence denotes Prior to initiation of DNA replication, Mcm helicase may adopt a double hexameric complex at the partially melted origin region, and may catalyze concurrent unwinding of duplex DNA into both directions, while stably associated with the origins of DNA replication.
T11281 34633-34638 JJ denotes Prior
T11282 34639-34641 IN denotes to
T11284 34642-34652 NN denotes initiation
T11285 34653-34655 IN denotes of
T11286 34656-34659 NN denotes DNA
T11287 34660-34671 NN denotes replication
T11288 34671-34673 , denotes ,
T11289 34673-34676 NN denotes Mcm
T11290 34677-34685 NN denotes helicase
T11291 34686-34689 MD denotes may
T11283 34690-34695 VB denotes adopt
T11292 34696-34697 DT denotes a
T11294 34698-34704 JJ denotes double
T11295 34705-34714 JJ denotes hexameric
T11293 34715-34722 NN denotes complex
T11296 34723-34725 IN denotes at
T11297 34726-34729 DT denotes the
T11299 34730-34739 RB denotes partially
T11300 34740-34746 VBN denotes melted
T11301 34747-34753 NN denotes origin
T11298 34754-34760 NN denotes region
T11302 34760-34762 , denotes ,
T11303 34762-34765 CC denotes and
T11304 34766-34769 MD denotes may
T11305 34770-34778 VB denotes catalyze
T11306 34779-34789 JJ denotes concurrent
T11307 34790-34799 NN denotes unwinding
T11308 34800-34802 IN denotes of
T11309 34803-34809 NN denotes duplex
T11310 34810-34813 NN denotes DNA
T11311 34814-34818 IN denotes into
T11312 34819-34823 DT denotes both
T11313 34824-34834 NNS denotes directions
T11314 34834-34836 , denotes ,
T11315 34836-34841 IN denotes while
T11317 34842-34848 RB denotes stably
T11316 34849-34859 VBN denotes associated
T11318 34860-34864 IN denotes with
T11319 34865-34868 DT denotes the
T11320 34869-34876 NNS denotes origins
T11321 34877-34879 IN denotes of
T11322 34880-34883 NN denotes DNA
T11323 34884-34895 NN denotes replication
T11324 34895-34896 . denotes .
T11325 34896-34986 sentence denotes This is similar to the T-antigen model originally proposed by Smelkova and Borowiec (21).
T11326 34897-34901 DT denotes This
T11327 34902-34904 VBZ denotes is
T11328 34905-34912 JJ denotes similar
T11329 34913-34915 IN denotes to
T11330 34916-34919 DT denotes the
T11332 34920-34921 NN denotes T
T11334 34921-34922 HYPH denotes -
T11333 34922-34929 NN denotes antigen
T11331 34930-34935 NN denotes model
T11335 34936-34946 RB denotes originally
T11336 34947-34955 VBN denotes proposed
T11337 34956-34958 IN denotes by
T11338 34959-34967 NNP denotes Smelkova
T11339 34968-34971 CC denotes and
T11340 34972-34980 NNP denotes Borowiec
T11341 34981-34982 -LRB- denotes (
T11342 34982-34984 CD denotes 21
T11343 34984-34985 -RRB- denotes )
T11344 34985-34986 . denotes .
T11345 34986-35175 sentence denotes We propose that, only when both hexamers are activated by the interacting thymine-rich sequences present within the melted region, the Mcm helicase is mobilized and initiation takes place.
T11346 34987-34989 PRP denotes We
T11347 34990-34997 VBP denotes propose
T11348 34998-35002 IN denotes that
T11350 35002-35004 , denotes ,
T11351 35004-35008 RB denotes only
T11353 35009-35013 WRB denotes when
T11354 35014-35018 DT denotes both
T11355 35019-35027 NNS denotes hexamers
T11356 35028-35031 VBP denotes are
T11352 35032-35041 VBN denotes activated
T11357 35042-35044 IN denotes by
T11358 35045-35048 DT denotes the
T11360 35049-35060 VBG denotes interacting
T11361 35061-35068 NN denotes thymine
T11363 35068-35069 HYPH denotes -
T11362 35069-35073 JJ denotes rich
T11359 35074-35083 NNS denotes sequences
T11364 35084-35091 JJ denotes present
T11365 35092-35098 IN denotes within
T11366 35099-35102 DT denotes the
T11368 35103-35109 VBN denotes melted
T11367 35110-35116 NN denotes region
T11369 35116-35118 , denotes ,
T11370 35118-35121 DT denotes the
T11372 35122-35125 NN denotes Mcm
T11371 35126-35134 NN denotes helicase
T11373 35135-35137 VBZ denotes is
T11349 35138-35147 VBN denotes mobilized
T11374 35148-35151 CC denotes and
T11375 35152-35162 NN denotes initiation
T11376 35163-35168 VBZ denotes takes
T11377 35169-35174 NN denotes place
T11378 35174-35175 . denotes .
T11379 35175-35304 sentence denotes A crucial question is how these double hexameric helicase complexes are generated and turn into active helicases at the origins.
T11380 35176-35177 DT denotes A
T11382 35178-35185 JJ denotes crucial
T11381 35186-35194 NN denotes question
T11383 35195-35197 VBZ denotes is
T11384 35198-35201 WRB denotes how
T11386 35202-35207 DT denotes these
T11388 35208-35214 RB denotes double
T11389 35215-35224 JJ denotes hexameric
T11390 35225-35233 NN denotes helicase
T11387 35234-35243 NNS denotes complexes
T11391 35244-35247 VBP denotes are
T11385 35248-35257 VBN denotes generated
T11392 35258-35261 CC denotes and
T11393 35262-35266 VB denotes turn
T11394 35267-35271 IN denotes into
T11395 35272-35278 JJ denotes active
T11396 35279-35288 NNS denotes helicases
T11397 35289-35291 IN denotes at
T11398 35292-35295 DT denotes the
T11399 35296-35303 NNS denotes origins
T11400 35303-35304 . denotes .
T11401 35304-35558 sentence denotes This process would involve melting of duplex DNA, which may be facilitated by binding of preRC components in the context of proper chromatin structures, or by other unwinding factors including a topoisomerase or a newly identified Mcm8 helicase (34–36).
T11402 35305-35309 DT denotes This
T11403 35310-35317 NN denotes process
T11405 35318-35323 MD denotes would
T11404 35324-35331 VB denotes involve
T11406 35332-35339 NN denotes melting
T11407 35340-35342 IN denotes of
T11408 35343-35349 NN denotes duplex
T11409 35350-35353 NN denotes DNA
T11410 35353-35355 , denotes ,
T11411 35355-35360 WDT denotes which
T11413 35361-35364 MD denotes may
T11414 35365-35367 VB denotes be
T11412 35368-35379 VBN denotes facilitated
T11415 35380-35382 IN denotes by
T11416 35383-35390 NN denotes binding
T11417 35391-35393 IN denotes of
T11418 35394-35399 NN denotes preRC
T11419 35400-35410 NNS denotes components
T11420 35411-35413 IN denotes in
T11421 35414-35417 DT denotes the
T11422 35418-35425 NN denotes context
T11423 35426-35428 IN denotes of
T11424 35429-35435 JJ denotes proper
T11426 35436-35445 NN denotes chromatin
T11425 35446-35456 NNS denotes structures
T11427 35456-35458 , denotes ,
T11428 35458-35460 CC denotes or
T11429 35461-35463 IN denotes by
T11430 35464-35469 JJ denotes other
T11432 35470-35479 JJ denotes unwinding
T11431 35480-35487 NNS denotes factors
T11433 35488-35497 VBG denotes including
T11434 35498-35499 DT denotes a
T11435 35500-35513 NN denotes topoisomerase
T11436 35514-35516 CC denotes or
T11437 35517-35518 DT denotes a
T11439 35519-35524 RB denotes newly
T11440 35525-35535 VBN denotes identified
T11441 35536-35540 NN denotes Mcm8
T11438 35541-35549 NN denotes helicase
T11442 35550-35551 -LRB- denotes (
T11443 35551-35553 CD denotes 34
T11444 35553-35554 SYM denotes –
T11445 35554-35556 CD denotes 36
T11446 35556-35557 -RRB- denotes )
T11447 35557-35558 . denotes .
T11448 35558-35706 sentence denotes It may also require association of fork-assisting proteins such as Cdc45 (37,38) as well as phosphorylation events by Cdk and Cdc7 kinases (39,40).
T11449 35559-35561 PRP denotes It
T11451 35562-35565 MD denotes may
T11452 35566-35570 RB denotes also
T11450 35571-35578 VB denotes require
T11453 35579-35590 NN denotes association
T11454 35591-35593 IN denotes of
T11455 35594-35598 NN denotes fork
T11457 35598-35599 HYPH denotes -
T11456 35599-35608 VBG denotes assisting
T11458 35609-35617 NN denotes proteins
T11459 35618-35622 JJ denotes such
T11460 35623-35625 IN denotes as
T11461 35626-35631 NN denotes Cdc45
T11462 35632-35633 -LRB- denotes (
T11464 35633-35635 CD denotes 37
T11465 35635-35636 , denotes ,
T11463 35636-35638 CD denotes 38
T11466 35638-35639 -RRB- denotes )
T11467 35640-35642 RB denotes as
T11469 35643-35647 RB denotes well
T11468 35648-35650 IN denotes as
T11470 35651-35666 NN denotes phosphorylation
T11471 35667-35673 NNS denotes events
T11472 35674-35676 IN denotes by
T11473 35677-35680 NN denotes Cdk
T11475 35681-35684 CC denotes and
T11476 35685-35689 NN denotes Cdc7
T11474 35690-35697 NNS denotes kinases
T11477 35698-35699 -LRB- denotes (
T11479 35699-35701 CD denotes 39
T11480 35701-35702 , denotes ,
T11478 35702-35704 CD denotes 40
T11481 35704-35705 -RRB- denotes )
T11482 35705-35706 . denotes .
T11483 35706-35803 sentence denotes One unexpected finding of this study is low helicase activity of Mcm4/6/7 on GC-rich duplex DNA.
T11484 35707-35710 CD denotes One
T11486 35711-35721 JJ denotes unexpected
T11485 35722-35729 NN denotes finding
T11488 35730-35732 IN denotes of
T11489 35733-35737 DT denotes this
T11490 35738-35743 NN denotes study
T11487 35744-35746 VBZ denotes is
T11491 35747-35750 JJ denotes low
T11493 35751-35759 NN denotes helicase
T11492 35760-35768 NN denotes activity
T11494 35769-35771 IN denotes of
T11495 35772-35776 NN denotes Mcm4
T11496 35776-35777 HYPH denotes /
T11497 35777-35778 CD denotes 6
T11498 35778-35779 HYPH denotes /
T11499 35779-35780 CD denotes 7
T11500 35781-35783 IN denotes on
T11501 35784-35786 NN denotes GC
T11503 35786-35787 HYPH denotes -
T11502 35787-35791 JJ denotes rich
T11505 35792-35798 NN denotes duplex
T11504 35799-35802 NN denotes DNA
T11506 35802-35803 . denotes .
T11507 35803-35988 sentence denotes This, in conjunction with the low processivity of Mcm4/6/7 helicase, strongly indicates that the replicative helicase at the replication forks would require more than Mcm4/6/7 complex.
T11508 35804-35808 DT denotes This
T11510 35808-35810 , denotes ,
T11511 35810-35812 IN denotes in
T11512 35813-35824 NN denotes conjunction
T11513 35825-35829 IN denotes with
T11514 35830-35833 DT denotes the
T11516 35834-35837 JJ denotes low
T11515 35838-35850 NN denotes processivity
T11517 35851-35853 IN denotes of
T11518 35854-35858 NN denotes Mcm4
T11520 35858-35859 HYPH denotes /
T11521 35859-35860 CD denotes 6
T11522 35860-35861 HYPH denotes /
T11523 35861-35862 CD denotes 7
T11519 35863-35871 NN denotes helicase
T11524 35871-35873 , denotes ,
T11525 35873-35881 RB denotes strongly
T11509 35882-35891 VBZ denotes indicates
T11526 35892-35896 IN denotes that
T11528 35897-35900 DT denotes the
T11530 35901-35912 JJ denotes replicative
T11529 35913-35921 NN denotes helicase
T11531 35922-35924 IN denotes at
T11532 35925-35928 DT denotes the
T11534 35929-35940 NN denotes replication
T11533 35941-35946 NNS denotes forks
T11535 35947-35952 MD denotes would
T11527 35953-35960 VB denotes require
T11536 35961-35965 JJR denotes more
T11537 35966-35970 IN denotes than
T11538 35971-35975 NN denotes Mcm4
T11540 35975-35976 HYPH denotes /
T11541 35976-35977 CD denotes 6
T11542 35977-35978 HYPH denotes /
T11543 35978-35979 CD denotes 7
T11539 35980-35987 NN denotes complex
T11544 35987-35988 . denotes .
T11545 35988-36327 sentence denotes During the processive unwinding of duplex DNA replication forks, Mcm4/6/7 may be further stimulated by interaction with other proteins, including remaining Mcm subunits, Cdc45 (37,38), GINS (41,42), DNA polymerase subunits (43), Mcm8 (35,36), etc. to become a truly processive and potent helicase capable of replicating the entire genome.
T11546 35989-35995 IN denotes During
T11548 35996-35999 DT denotes the
T11550 36000-36010 JJ denotes processive
T11549 36011-36020 NN denotes unwinding
T11551 36021-36023 IN denotes of
T11552 36024-36030 NN denotes duplex
T11554 36031-36034 NN denotes DNA
T11555 36035-36046 NN denotes replication
T11553 36047-36052 NNS denotes forks
T11556 36052-36054 , denotes ,
T11557 36054-36058 NN denotes Mcm4
T11558 36058-36059 HYPH denotes /
T11559 36059-36060 CD denotes 6
T11560 36060-36061 HYPH denotes /
T11561 36061-36062 CD denotes 7
T11562 36063-36066 MD denotes may
T11563 36067-36069 VB denotes be
T11564 36070-36077 RB denotes further
T11547 36078-36088 VBN denotes stimulated
T11565 36089-36091 IN denotes by
T11566 36092-36103 NN denotes interaction
T11567 36104-36108 IN denotes with
T11568 36109-36114 JJ denotes other
T11569 36115-36123 NN denotes proteins
T11570 36123-36125 , denotes ,
T11571 36125-36134 VBG denotes including
T11572 36135-36144 VBG denotes remaining
T11574 36145-36148 NN denotes Mcm
T11573 36149-36157 NNS denotes subunits
T11575 36157-36159 , denotes ,
T11576 36159-36164 NN denotes Cdc45
T11577 36165-36166 -LRB- denotes (
T11579 36166-36168 CD denotes 37
T11580 36168-36169 , denotes ,
T11578 36169-36171 CD denotes 38
T11581 36171-36172 -RRB- denotes )
T11582 36172-36174 , denotes ,
T11583 36174-36178 NN denotes GINS
T11584 36179-36180 -LRB- denotes (
T11586 36180-36182 CD denotes 41
T11587 36182-36183 , denotes ,
T11585 36183-36185 CD denotes 42
T11588 36185-36186 -RRB- denotes )
T11589 36186-36188 , denotes ,
T11590 36188-36191 NN denotes DNA
T11592 36192-36202 NN denotes polymerase
T11591 36203-36211 NNS denotes subunits
T11593 36212-36213 -LRB- denotes (
T11594 36213-36215 CD denotes 43
T11595 36215-36216 -RRB- denotes )
T11596 36216-36218 , denotes ,
T11597 36218-36222 NN denotes Mcm8
T11598 36223-36224 -LRB- denotes (
T11600 36224-36226 CD denotes 35
T11601 36226-36227 , denotes ,
T11599 36227-36229 CD denotes 36
T11602 36229-36230 -RRB- denotes )
T11603 36230-36232 , denotes ,
T11604 36232-36236 FW denotes etc.
T11605 36237-36239 TO denotes to
T11606 36240-36246 VB denotes become
T11607 36247-36248 DT denotes a
T11609 36249-36254 RB denotes truly
T11610 36255-36265 JJ denotes processive
T11611 36266-36269 CC denotes and
T11612 36270-36276 JJ denotes potent
T11608 36277-36285 NN denotes helicase
T11613 36286-36293 JJ denotes capable
T11614 36294-36296 IN denotes of
T11615 36297-36308 VBG denotes replicating
T11616 36309-36312 DT denotes the
T11618 36313-36319 JJ denotes entire
T11617 36320-36326 NN denotes genome
T11619 36326-36327 . denotes .
R1000 T1673 T1672 nmod Mcm4,complex
R1001 T1674 T1673 punct /,Mcm4
R1002 T1675 T1673 nummod 6,Mcm4
R1003 T1676 T1673 punct /,Mcm4
R1004 T1677 T1673 nummod 7,Mcm4
R1005 T1679 T1680 det The,baculoviruses
R1006 T1680 T1682 nsubjpass baculoviruses,described
R1007 T1681 T1680 amod recombinant,baculoviruses
R1008 T1683 T1680 acl expressing,baculoviruses
R1009 T1684 T1685 nmod His6,Mcm6
R1010 T1685 T1689 nmod Mcm6,proteins
R1011 T1686 T1685 punct -,Mcm6
R1012 T1687 T1685 nmod Mcm4,Mcm6
R1013 T1688 T1685 punct /,Mcm6
R1014 T1689 T1683 dobj proteins,expressing
R1015 T1690 T1685 cc or,Mcm6
R1016 T1691 T1692 compound Mcm7,FLAG
R1017 T1692 T1685 conj FLAG,Mcm6
R1018 T1693 T1692 punct -,FLAG
R1019 T1694 T1682 auxpass were,described
R1020 T1695 T1682 advmod previously,described
R1021 T1696 T1697 punct (,11
R1022 T1697 T1682 parataxis 11,described
R1023 T1698 T1697 punct ),11
R1024 T1699 T1682 punct .,described
R1025 T1701 T1702 nmod Sf9,cells
R1026 T1702 T1707 nsubjpass cells,cultured
R1027 T1703 T1701 cc and,Sf9
R1028 T1704 T1705 amod High,five
R1029 T1705 T1701 conj five,Sf9
R1030 T1706 T1702 compound insect,cells
R1031 T1708 T1707 auxpass were,cultured
R1032 T1709 T1707 prep at,cultured
R1033 T1710 T1711 nummod 27,°C
R1034 T1711 T1709 pobj °C,at
R1035 T1712 T1707 prep in,cultured
R1036 T1713 T1714 nmod Sf,SFM
R1037 T1714 T1712 pobj SFM,in
R1038 T1715 T1713 punct -,Sf
R1039 T1716 T1713 nummod 900,Sf
R1040 T1717 T1714 nummod II,SFM
R1041 T1718 T1719 punct (,Inc.
R1042 T1719 T1714 parataxis Inc.,SFM
R1043 T1720 T1719 nmod Life,Inc.
R1044 T1721 T1719 nmod Technologies,Inc.
R1045 T1722 T1719 punct ", ",Inc.
R1046 T1723 T1719 punct ),Inc.
R1047 T1724 T1714 cc and,SFM
R1048 T1725 T1726 nmod EX,CELL
R1049 T1726 T1728 nmod CELL,medium
R1050 T1727 T1726 punct -,CELL
R1051 T1728 T1714 conj medium,SFM
R1052 T1729 T1726 nummod 405,CELL
R1053 T1730 T1731 punct (,Biosciences
R1054 T1731 T1728 parataxis Biosciences,medium
R1055 T1732 T1731 compound JRH,Biosciences
R1056 T1733 T1731 punct ),Biosciences
R1057 T1734 T1707 punct ", ",cultured
R1058 T1735 T1707 advmod respectively,cultured
R1059 T1736 T1707 punct .,cultured
R1060 T1738 T1739 prep For,coinfected
R1061 T1740 T1738 pobj expression,For
R1062 T1741 T1740 prep of,expression
R1063 T1742 T1743 det the,proteins
R1064 T1743 T1741 pobj proteins,of
R1065 T1744 T1743 nmod Mcm4,proteins
R1066 T1745 T1744 punct /,Mcm4
R1067 T1746 T1744 nummod 6,Mcm4
R1068 T1747 T1744 punct /,Mcm4
R1069 T1748 T1744 nummod 7,Mcm4
R1070 T1749 T1739 punct ", ",coinfected
R1071 T1750 T1751 amod High,five
R1072 T1751 T1752 nummod five,cells
R1073 T1752 T1739 nsubjpass cells,coinfected
R1074 T1753 T1739 auxpass were,coinfected
R1075 T1754 T1739 prep with,coinfected
R1076 T1755 T1756 amod recombinant,baculoviruses
R1077 T1756 T1754 pobj baculoviruses,with
R1078 T1757 T1756 acl expressing,baculoviruses
R1079 T1758 T1759 det the,proteins
R1080 T1759 T1757 dobj proteins,expressing
R1081 T1760 T1761 compound His6,Mcm6
R1082 T1761 T1759 compound Mcm6,proteins
R1083 T1762 T1761 punct -,Mcm6
R1084 T1763 T1761 compound Mcm4,Mcm6
R1085 T1764 T1761 punct /,Mcm6
R1086 T1765 T1756 cc and,baculoviruses
R1087 T1766 T1756 conj those,baculoviruses
R1088 T1767 T1766 acl expressing,those
R1089 T1768 T1769 det the,FLAG
R1090 T1769 T1767 dobj FLAG,expressing
R1091 T1770 T1769 compound Mcm7,FLAG
R1092 T1771 T1769 punct -,FLAG
R1093 T1772 T1739 punct ", ",coinfected
R1094 T1773 T1739 cc and,coinfected
R1095 T1774 T1775 auxpass were,collected
R1096 T1775 T1739 conj collected,coinfected
R1097 T1776 T1775 prep at,collected
R1098 T1777 T1778 nummod 48,h
R1099 T1778 T1776 pobj h,at
R1100 T1779 T1778 advmod post-infection,h
R1101 T1780 T1739 punct .,coinfected
R1102 T1782 T1783 det The,complexes
R1103 T1783 T1790 nsubjpass complexes,purified
R1104 T1784 T1783 amod recombinant,complexes
R1105 T1785 T1783 nmod Mcm4,complexes
R1106 T1786 T1785 punct /,Mcm4
R1107 T1787 T1785 nummod 6,Mcm4
R1108 T1788 T1785 punct /,Mcm4
R1109 T1789 T1785 nummod 7,Mcm4
R1110 T1791 T1790 auxpass were,purified
R1111 T1792 T1793 mark as,described
R1112 T1793 T1790 advcl described,purified
R1113 T1794 T1793 advmod previously,described
R1114 T1795 T1796 punct (,12
R1115 T1796 T1793 parataxis 12,described
R1116 T1797 T1796 punct ),12
R1117 T1798 T1790 punct .,purified
R1124 T1980 T1981 compound DNA,substrates
R1125 T1983 T1984 det The,sequences
R1126 T1984 T1985 nsubjpass sequences,listed
R1127 T1986 T1984 prep for,sequences
R1128 T1987 T1988 predet all,oligonucleotides
R1129 T1988 T1986 pobj oligonucleotides,for
R1130 T1989 T1988 det the,oligonucleotides
R1131 T1990 T1988 acl used,oligonucleotides
R1132 T1991 T1990 prep for,used
R1133 T1992 T1991 pobj constructions,for
R1134 T1993 T1992 prep of,constructions
R1135 T1994 T1995 compound DNA,substrates
R1136 T1995 T1993 pobj substrates,of
R1137 T1996 T1985 auxpass are,listed
R1138 T1997 T1985 prep in,listed
R1139 T1998 T1999 nmod Tables,1
R1140 T1999 T1997 pobj 1,in
R1141 T2000 T1999 cc and,1
R1142 T2001 T1999 conj 2,1
R1143 T2002 T1985 punct .,listed
R1144 T2004 T2005 det The,substrates
R1145 T2005 T2008 nsubjpass substrates,constructed
R1146 T2006 T2005 amod partial,substrates
R1147 T2007 T2005 compound heteroduplex,substrates
R1148 T2009 T2008 auxpass were,constructed
R1149 T2010 T2008 prep by,constructed
R1150 T2011 T2010 pcomp annealing,by
R1151 T2012 T2013 amod labeled,oligonucleotides
R1152 T2013 T2011 dobj oligonucleotides,annealing
R1153 T2014 T2011 prep with,annealing
R1154 T2015 T2016 nmod M13mp18,DNA
R1155 T2016 T2014 pobj DNA,with
R1156 T2017 T2018 amod single,stranded
R1157 T2018 T2016 amod stranded,DNA
R1158 T2019 T2018 punct -,stranded
R1159 T2020 T2016 amod circular,DNA
R1160 T2021 T2016 cc or,DNA
R1161 T2022 T2023 poss its,derivative
R1162 T2023 T2016 conj derivative,DNA
R1163 T2024 T2008 punct .,constructed
R1164 T2026 T2027 det The,substrates
R1165 T2027 T2029 nsubjpass substrates,purified
R1166 T2028 T2027 amod labeled,substrates
R1167 T2030 T2029 auxpass were,purified
R1168 T2031 T2029 agent by,purified
R1169 T2032 T2033 compound Sepharose,CL4B
R1170 T2033 T2034 compound CL4B,chromatography
R1171 T2034 T2031 pobj chromatography,by
R1172 T2035 T2034 compound column,chromatography
R1173 T2036 T2037 punct (,Biotech
R1174 T2037 T2029 parataxis Biotech,purified
R1175 T2038 T2037 compound Amersham,Biotech
R1176 T2039 T2037 compound Pharmacia,Biotech
R1177 T2040 T2037 punct ),Biotech
R1178 T2041 T2029 punct .,purified
R1179 T2043 T2044 det The,substrates
R1180 T2044 T2047 nsubjpass substrates,assembled
R1181 T2045 T2046 compound DNA,bubble
R1182 T2046 T2044 compound bubble,substrates
R1183 T2048 T2047 auxpass were,assembled
R1184 T2049 T2047 prep from,assembled
R1185 T2050 T2051 nummod two,oligonucleotides
R1186 T2051 T2049 pobj oligonucleotides,from
R1187 T2052 T2053 advmod partially,complementary
R1188 T2053 T2051 amod complementary,oligonucleotides
R1189 T2054 T2051 prep with,oligonucleotides
R1190 T2055 T2056 amod top,sequences
R1191 T2056 T2054 pobj sequences,with
R1192 T2057 T2055 cc and,top
R1193 T2058 T2055 conj bottom,top
R1194 T2059 T2056 compound strand,sequences
R1195 T2060 T2061 punct (,Table
R1196 T2061 T2051 parataxis Table,oligonucleotides
R1197 T2062 T2061 nummod 1,Table
R1198 T2063 T2061 punct ),Table
R1199 T2064 T2047 punct .,assembled
R1200 T2066 T2067 npadvmod T,tailed
R1201 T2067 T2069 amod tailed,substrates
R1202 T2068 T2067 punct -,tailed
R1203 T2069 T2073 nsubjpass substrates,prepared
R1204 T2070 T2071 compound Y,fork
R1205 T2071 T2069 compound fork,substrates
R1206 T2072 T2071 punct -,fork
R1207 T2074 T2073 auxpass were,prepared
R1208 T2075 T2073 prep by,prepared
R1209 T2076 T2075 pcomp annealing,by
R1210 T2077 T2078 nummod two,oligonucleotides
R1211 T2078 T2076 dobj oligonucleotides,annealing
R1212 T2079 T2080 punct (,series
R1213 T2080 T2078 parataxis series,oligonucleotides
R1214 T2081 T2080 nmod dT30,series
R1215 T2082 T2081 cc and,dT30
R1216 T2083 T2081 conj dT60,dT30
R1217 T2084 T2080 punct ),series
R1218 T2085 T2073 punct .,prepared
R1219 T2087 T2088 aux To,construct
R1220 T2088 T2089 advcl construct,annealed
R1221 T2090 T2091 det the,fork
R1222 T2091 T2088 dobj fork,construct
R1223 T2092 T2091 amod arrested,fork
R1224 T2093 T2091 cc and,fork
R1225 T2094 T2095 nummod 3,substrates
R1226 T2095 T2091 conj substrates,fork
R1227 T2096 T2094 punct ′,3
R1228 T2097 T2094 punct -,3
R1229 T2098 T2094 cc or,3
R1230 T2099 T2094 conj 5,3
R1231 T2100 T2099 punct ′,5
R1232 T2101 T2095 punct -,substrates
R1233 T2102 T2095 compound extension,substrates
R1234 T2103 T2089 punct ", ",annealed
R1235 T2104 T2105 amod various,combinations
R1236 T2105 T2089 nsubjpass combinations,annealed
R1237 T2106 T2105 prep of,combinations
R1238 T2107 T2106 pobj oligonucleotides,of
R1239 T2108 T2107 acl shown,oligonucleotides
R1240 T2109 T2108 prep in,shown
R1241 T2110 T2109 pobj Table,in
R1242 T2111 T2110 nummod 1,Table
R1243 T2112 T2089 auxpass were,annealed
R1244 T2113 T2089 punct .,annealed
R1245 T2115 T2116 nmod Bub,B2
R1246 T2116 T2121 nsubjpass B2,constructed
R1247 T2117 T2116 punct -,B2
R1248 T2118 T2116 nummod 82,B2
R1249 T2119 T2116 punct /,B2
R1250 T2120 T2116 compound lamin,B2
R1251 T2122 T2116 acl containing,B2
R1252 T2123 T2124 det the,strand
R1253 T2124 T2122 dobj strand,containing
R1254 T2125 T2126 npadvmod T,rich
R1255 T2126 T2124 amod rich,strand
R1256 T2127 T2126 punct -,rich
R1257 T2128 T2124 prep of,strand
R1258 T2129 T2130 det the,region
R1259 T2130 T2128 pobj region,of
R1260 T2131 T2132 compound lamin,B2
R1261 T2132 T2130 compound B2,region
R1262 T2133 T2130 compound origin,region
R1263 T2134 T2121 auxpass was,constructed
R1264 T2135 T2136 mark as,described
R1265 T2136 T2121 advcl described,constructed
R1266 T2137 T2136 advmod previously,described
R1267 T2138 T2139 punct (,13
R1268 T2139 T2136 parataxis 13,described
R1269 T2140 T2139 punct ),13
R1270 T2141 T2121 punct .,constructed
R1271 T2143 T2144 det The,substrate
R1272 T2144 T2146 nsubj substrate,contains
R1273 T2145 T2144 compound bubble,substrate
R1274 T2147 T2144 punct ", ",substrate
R1275 T2148 T2149 compound c,myc
R1276 T2149 T2151 compound myc,DUE
R1277 T2150 T2149 punct -,myc
R1278 T2151 T2144 appos DUE,substrate
R1279 T2152 T2151 punct /,DUE
R1280 T2153 T2151 cc or,DUE
R1281 T2154 T2155 compound c,myc
R1282 T2155 T2157 compound myc,C
R1283 T2156 T2155 punct -,myc
R1284 T2157 T2151 conj C,DUE
R1285 T2158 T2157 punct /,C
R1286 T2159 T2157 compound DUE,C
R1287 T2160 T2157 punct -,C
R1288 T2161 T2146 punct ", ",contains
R1289 T2162 T2146 dobj sequences,contains
R1290 T2163 T2164 punct (,K01908
R1291 T2164 T2162 parataxis K01908,sequences
R1292 T2165 T2166 compound GenBank,number
R1293 T2166 T2164 compound number,K01908
R1294 T2167 T2166 compound accession,number
R1295 T2168 T2164 punct ),K01908
R1296 T2169 T2162 prep from,sequences
R1297 T2170 T2171 nmod nucleotides,853
R1298 T2171 T2169 pobj 853,from
R1299 T2172 T2171 nummod 722,853
R1300 T2173 T2171 nmod to,853
R1301 T2174 T2162 cc or,sequences
R1302 T2175 T2162 conj those,sequences
R1303 T2176 T2175 prep from,those
R1304 T2177 T2176 pobj 828,from
R1305 T2178 T2176 prep to,from
R1306 T2179 T2178 pobj 747,to
R1307 T2180 T2181 punct (,strand
R1308 T2181 T2175 parataxis strand,those
R1309 T2182 T2181 amod complementary,strand
R1310 T2183 T2181 punct ),strand
R1311 T2184 T2146 punct ", ",contains
R1312 T2185 T2146 advmod respectively,contains
R1313 T2186 T2146 punct ", ",contains
R1314 T2187 T2146 prep in,contains
R1315 T2188 T2189 det the,region
R1316 T2189 T2187 pobj region,in
R1317 T2190 T2189 amod central,region
R1318 T2191 T2189 amod melted,region
R1319 T2192 T2146 punct .,contains
R1320 T2194 T2195 prep For,annealed
R1321 T2196 T2197 det each,substrate
R1322 T2197 T2194 pobj substrate,For
R1323 T2198 T2195 punct ", ",annealed
R1324 T2199 T2200 amod labeled,oligonucleotide
R1325 T2200 T2195 nsubjpass oligonucleotide,annealed
R1326 T2201 T2202 punct (,pmol
R1327 T2202 T2200 parataxis pmol,oligonucleotide
R1328 T2203 T2202 nummod 3,pmol
R1329 T2204 T2202 punct ),pmol
R1330 T2205 T2195 auxpass was,annealed
R1331 T2206 T2195 prep with,annealed
R1332 T2207 T2208 det the,oligonucleotide
R1333 T2208 T2206 pobj oligonucleotide,with
R1334 T2209 T2208 amod unlabeled,oligonucleotide
R1335 T2210 T2211 punct (,pmol
R1336 T2211 T2208 parataxis pmol,oligonucleotide
R1337 T2212 T2211 nummod 6,pmol
R1338 T2213 T2211 punct ),pmol
R1339 T2214 T2195 prep in,annealed
R1340 T2215 T2216 det a,mixture
R1341 T2216 T2214 pobj mixture,in
R1342 T2217 T2216 compound reaction,mixture
R1343 T2218 T2219 punct (,μl
R1344 T2219 T2216 parataxis μl,mixture
R1345 T2220 T2219 nummod 50,μl
R1346 T2221 T2219 punct ),μl
R1347 T2222 T2216 acl containing,mixture
R1348 T2223 T2224 nummod 20,mM
R1349 T2224 T2225 compound mM,HCl
R1350 T2225 T2222 dobj HCl,containing
R1351 T2226 T2225 compound Tris,HCl
R1352 T2227 T2225 punct –,HCl
R1353 T2228 T2229 punct (,pH
R1354 T2229 T2225 parataxis pH,HCl
R1355 T2230 T2229 nummod 7.5,pH
R1356 T2231 T2229 punct ),pH
R1357 T2232 T2225 punct ", ",HCl
R1358 T2233 T2234 nummod 10,mM
R1359 T2234 T2235 compound mM,MgCl2
R1360 T2235 T2225 conj MgCl2,HCl
R1361 T2236 T2235 cc and,MgCl2
R1362 T2237 T2238 nummod 25,mM
R1363 T2238 T2239 compound mM,NaCl
R1364 T2239 T2235 conj NaCl,MgCl2
R1365 T2240 T2216 punct ", ",mixture
R1366 T2241 T2242 dep which,heated
R1367 T2242 T2216 relcl heated,mixture
R1368 T2243 T2242 auxpass were,heated
R1369 T2244 T2242 prep to,heated
R1370 T2245 T2246 nummod 95,°C
R1371 T2246 T2244 pobj °C,to
R1372 T2247 T2242 punct ", ",heated
R1373 T2248 T2242 conj kept,heated
R1374 T2249 T2248 prep at,kept
R1375 T2250 T2251 nummod 67,°C
R1376 T2251 T2249 pobj °C,at
R1377 T2252 T2248 prep for,kept
R1378 T2253 T2254 nummod 1,h
R1379 T2254 T2252 pobj h,for
R1380 T2255 T2248 punct ", ",kept
R1381 T2256 T2248 cc and,kept
R1382 T2257 T2258 advmod then,allowed
R1383 T2258 T2248 conj allowed,kept
R1384 T2259 T2260 aux to,cool
R1385 T2260 T2258 xcomp cool,allowed
R1386 T2261 T2260 advmod slowly,cool
R1387 T2262 T2260 prt down,cool
R1388 T2263 T2260 prep to,cool
R1389 T2264 T2265 nummod 37,°C
R1390 T2265 T2263 pobj °C,to
R1391 T2266 T2195 punct .,annealed
R1392 T2268 T2269 det The,substrates
R1393 T2269 T2271 nsubjpass substrates,purified
R1394 T2270 T2269 amod assembled,substrates
R1395 T2272 T2271 auxpass were,purified
R1396 T2273 T2271 prep from,purified
R1397 T2274 T2275 compound polyacrylamide,gel
R1398 T2275 T2273 pobj gel,from
R1399 T2276 T2271 prep by,purified
R1400 T2277 T2276 pobj elution,by
R1401 T2278 T2277 prep into,elution
R1402 T2279 T2280 compound TE,buffer
R1403 T2280 T2278 pobj buffer,into
R1404 T2281 T2282 punct (,24
R1405 T2282 T2271 parataxis 24,purified
R1406 T2283 T2282 punct ),24
R1407 T2284 T2271 punct .,purified
R1408 T2286 T2287 det The,substrates
R1409 T2287 T2290 nsubjpass substrates,prepared
R1410 T2288 T2287 amod partial,substrates
R1411 T2289 T2287 compound heteroduplex,substrates
R1412 T2291 T2287 prep on,substrates
R1413 T2292 T2293 det a,DNA
R1414 T2293 T2291 pobj DNA,on
R1415 T2294 T2295 amod single,stranded
R1416 T2295 T2293 amod stranded,DNA
R1417 T2296 T2295 punct -,stranded
R1418 T2297 T2293 amod circular,DNA
R1419 T2298 T2293 prep with,DNA
R1420 T2299 T2300 amod varied,lengths
R1421 T2300 T2298 pobj lengths,with
R1422 T2301 T2300 prep of,lengths
R1423 T2302 T2303 compound duplex,regions
R1424 T2303 T2301 pobj regions,of
R1425 T2304 T2290 auxpass were,prepared
R1426 T2305 T2290 prep by,prepared
R1427 T2306 T2305 pcomp extending,by
R1428 T2307 T2308 compound DNA,chains
R1429 T2308 T2306 dobj chains,extending
R1430 T2309 T2306 prep from,extending
R1431 T2310 T2311 det the,end
R1432 T2311 T2309 pobj end,from
R1433 T2312 T2311 nummod 3,end
R1434 T2313 T2312 punct ′,3
R1435 T2314 T2311 prep of,end
R1436 T2315 T2316 det the,37mer
R1437 T2316 T2314 pobj 37mer,of
R1438 T2317 T2316 compound dT40,37mer
R1439 T2318 T2316 punct -,37mer
R1440 T2319 T2316 punct (,37mer
R1441 T2320 T2321 compound 37mer,region
R1442 T2321 T2316 appos region,37mer
R1443 T2322 T2321 acl hybridizing,region
R1444 T2323 T2322 prep at,hybridizing
R1445 T2324 T2325 nmod positions,6289
R1446 T2325 T2323 pobj 6289,at
R1447 T2326 T2327 punct –,6326
R1448 T2327 T2325 prep 6326,6289
R1449 T2328 T2325 prep of,6289
R1450 T2329 T2330 compound M13mp18,vector
R1451 T2330 T2328 pobj vector,of
R1452 T2331 T2316 punct ),37mer
R1453 T2332 T2316 acl annealed,37mer
R1454 T2333 T2332 prep to,annealed
R1455 T2334 T2335 det the,DNA
R1456 T2335 T2333 pobj DNA,to
R1457 T2336 T2337 amod single,stranded
R1458 T2337 T2335 amod stranded,DNA
R1459 T2338 T2337 punct -,stranded
R1460 T2339 T2335 amod circular,DNA
R1461 T2340 T2290 punct .,prepared
R1462 T2342 T2343 compound DNA,chains
R1463 T2343 T2344 nsubjpass chains,elongated
R1464 T2345 T2344 auxpass were,elongated
R1465 T2346 T2344 prep with,elongated
R1466 T2347 T2346 pobj Sequenase,with
R1467 T2348 T2344 prep in,elongated
R1468 T2349 T2350 det the,presence
R1469 T2350 T2348 pobj presence,in
R1470 T2351 T2350 prep of,presence
R1471 T2352 T2353 punct [,dGTP
R1472 T2353 T2351 pobj dGTP,of
R1473 T2354 T2355 nmod α,32P
R1474 T2355 T2353 nmod 32P,dGTP
R1475 T2356 T2355 punct -,32P
R1476 T2357 T2353 punct ],dGTP
R1477 T2358 T2353 punct ", ",dGTP
R1478 T2359 T2353 acl followed,dGTP
R1479 T2360 T2359 agent by,followed
R1480 T2361 T2360 pobj extension,by
R1481 T2362 T2359 prep after,followed
R1482 T2363 T2362 pobj addition,after
R1483 T2364 T2363 prep of,addition
R1484 T2365 T2364 pobj ddGTP,of
R1485 T2366 T2365 cc and,ddGTP
R1486 T2367 T2368 det all,dNTPs
R1487 T2368 T2365 conj dNTPs,ddGTP
R1488 T2369 T2368 nummod four,dNTPs
R1489 T2370 T2344 punct ", ",elongated
R1490 T2371 T2344 advcl resulting,elongated
R1491 T2372 T2371 prep in,resulting
R1492 T2373 T2372 pobj substrates,in
R1493 T2374 T2373 acl containing,substrates
R1494 T2375 T2376 amod labeled,regions
R1495 T2376 T2374 dobj regions,containing
R1496 T2377 T2376 compound duplex,regions
R1497 T2378 T2376 prep of,regions
R1498 T2379 T2380 amod varied,lengths
R1499 T2380 T2378 pobj lengths,of
R1500 T2381 T2382 punct (,13
R1501 T2382 T2344 parataxis 13,elongated
R1502 T2383 T2382 punct ),13
R1503 T2384 T2344 punct .,elongated
R1504 T2386 T2387 det The,substrate
R1505 T2387 T2388 nsubjpass substrate,incubated
R1506 T2389 T2390 punct (,fmol
R1507 T2390 T2387 parataxis fmol,substrate
R1508 T2391 T2390 nummod 5,fmol
R1509 T2392 T2390 punct ),fmol
R1510 T2393 T2388 auxpass was,incubated
R1511 T2394 T2388 advmod first,incubated
R1512 T2395 T2388 prep with,incubated
R1513 T2396 T2397 amod indicated,amount
R1514 T2397 T2395 pobj amount,with
R1515 T2398 T2397 prep of,amount
R1516 T2399 T2400 det the,complex
R1517 T2400 T2398 pobj complex,of
R1518 T2401 T2400 nmod Mcm4,complex
R1519 T2402 T2401 punct /,Mcm4
R1520 T2403 T2401 nummod 6,Mcm4
R1521 T2404 T2401 punct /,Mcm4
R1522 T2405 T2401 nummod 7,Mcm4
R1523 T2406 T2388 prep at,incubated
R1524 T2407 T2408 nummod 37,°C
R1525 T2408 T2406 pobj °C,at
R1526 T2409 T2388 prep for,incubated
R1527 T2410 T2411 nummod 1,h
R1528 T2411 T2409 pobj h,for
R1529 T2412 T2388 prep in,incubated
R1530 T2413 T2414 compound reaction,mixture
R1531 T2414 T2412 pobj mixture,in
R1532 T2415 T2416 mark as,described
R1533 T2416 T2388 advcl described,incubated
R1534 T2417 T2418 punct (,11
R1535 T2418 T2416 parataxis 11,described
R1536 T2419 T2418 punct ),11
R1537 T2420 T2388 punct ", ",incubated
R1538 T2421 T2388 cc and,incubated
R1539 T2422 T2421 advmod then,and
R1540 T2423 T2424 nsubjpass reactions,stopped
R1541 T2424 T2388 conj stopped,incubated
R1542 T2425 T2424 auxpass were,stopped
R1543 T2426 T2424 agent by,stopped
R1544 T2427 T2426 pobj addition,by
R1545 T2428 T2427 prep of,addition
R1546 T2429 T2428 pobj EDTA,of
R1547 T2430 T2431 punct (,mM
R1548 T2431 T2429 parataxis mM,EDTA
R1549 T2432 T2431 nummod 20,mM
R1550 T2433 T2431 punct ),mM
R1551 T2434 T2429 punct ", ",EDTA
R1552 T2435 T2429 conj SDS,EDTA
R1553 T2436 T2437 punct (,%
R1554 T2437 T2435 parataxis %,SDS
R1555 T2438 T2437 nummod 0.1,%
R1556 T2439 T2437 punct ),%
R1557 T2440 T2435 cc and,SDS
R1558 T2441 T2442 nummod 2,μg
R1559 T2442 T2443 compound μg,K
R1560 T2443 T2435 conj K,SDS
R1561 T2444 T2443 compound proteinase,K
R1562 T2445 T2427 punct ", ",addition
R1563 T2446 T2427 acl followed,addition
R1564 T2447 T2446 agent by,followed
R1565 T2448 T2447 pobj incubation,by
R1566 T2449 T2448 prep for,incubation
R1567 T2450 T2451 nummod 20,min
R1568 T2451 T2449 pobj min,for
R1569 T2452 T2424 punct .,stopped
R1570 T2454 T2455 nsubjpass Aliquots,electrophoresed
R1571 T2456 T2455 auxpass were,electrophoresed
R1572 T2457 T2455 prep on,electrophoresed
R1573 T2458 T2459 det a,gel
R1574 T2459 T2457 pobj gel,on
R1575 T2460 T2461 nummod 6.5,%
R1576 T2461 T2459 compound %,gel
R1577 T2462 T2459 compound polyacrylamide,gel
R1578 T2463 T2455 prep in,electrophoresed
R1579 T2464 T2465 nummod 1,TBE
R1580 T2465 T2463 pobj TBE,in
R1581 T2466 T2464 punct ×,1
R1582 T2467 T2455 prep at,electrophoresed
R1583 T2468 T2469 nummod 300,V
R1584 T2469 T2467 pobj V,at
R1585 T2470 T2455 prep for,electrophoresed
R1586 T2471 T2472 nummod 2.5,h
R1587 T2472 T2470 pobj h,for
R1588 T2473 T2455 punct .,electrophoresed
R1589 T2475 T2476 det The,DNAs
R1590 T2476 T2481 nsubj DNAs,were
R1591 T2477 T2478 amod single,stranded
R1592 T2478 T2476 amod stranded,DNAs
R1593 T2479 T2478 punct -,stranded
R1594 T2480 T2476 amod circular,DNAs
R1595 T2482 T2476 acl used,DNAs
R1596 T2483 T2481 attr M13mp18,were
R1597 T2484 T2483 cc and,M13mp18
R1598 T2485 T2483 conj M13mp19,M13mp18
R1599 T2486 T2483 punct +,M13mp18
R1600 T2487 T2483 appos G,M13mp18
R1601 T2488 T2487 punct -,G
R1602 T2489 T2487 amod rich,G
R1603 T2490 T2481 punct .,were
R1604 T2492 T2493 det The,latter
R1605 T2493 T2494 nsubjpass latter,constructed
R1606 T2495 T2494 auxpass was,constructed
R1607 T2496 T2494 prep by,constructed
R1608 T2497 T2496 pcomp inserting,by
R1609 T2498 T2499 det a,sequence
R1610 T2499 T2497 dobj sequence,inserting
R1611 T2500 T2499 nmod G,sequence
R1612 T2501 T2500 punct -,G
R1613 T2502 T2500 amod rich,G
R1614 T2503 T2499 compound repeat,sequence
R1615 T2504 T2499 punct (,sequence
R1616 T2505 T2499 appos GGGGCGTGGGC,sequence
R1617 T2506 T2505 punct ),GGGGCGTGGGC
R1618 T2507 T2505 nummod 6,GGGGCGTGGGC
R1619 T2508 T2499 punct ", ",sequence
R1620 T2509 T2499 acl prepared,sequence
R1621 T2510 T2509 prep by,prepared
R1622 T2511 T2510 pcomp annealing,by
R1623 T2512 T2511 prep of,annealing
R1624 T2513 T2514 nummod two,oligonucleotides
R1625 T2514 T2512 pobj oligonucleotides,of
R1626 T2515 T2514 punct (,oligonucleotides
R1627 T2516 T2514 appos 5,oligonucleotides
R1628 T2517 T2516 punct ′,5
R1629 T2518 T2516 punct -,5
R1630 T2519 T2520 nmod GATCC,GGGGCGTGGGC
R1631 T2520 T2516 appos GGGGCGTGGGC,5
R1632 T2521 T2520 punct -,GGGGCGTGGGC
R1633 T2522 T2520 punct [,GGGGCGTGGGC
R1634 T2523 T2520 punct ],GGGGCGTGGGC
R1635 T2524 T2520 nummod 6,GGGGCGTGGGC
R1636 T2525 T2516 punct -,5
R1637 T2526 T2516 nummod 3,5
R1638 T2527 T2516 punct ′,5
R1639 T2528 T2516 punct /,5
R1640 T2529 T2516 appos 5,5
R1641 T2530 T2529 punct ′,5
R1642 T2531 T2529 punct -,5
R1643 T2532 T2533 nmod GATCC,GCCCACGCCCC
R1644 T2533 T2529 appos GCCCACGCCCC,5
R1645 T2534 T2533 punct -,GCCCACGCCCC
R1646 T2535 T2533 punct [,GCCCACGCCCC
R1647 T2536 T2533 punct ],GCCCACGCCCC
R1648 T2537 T2533 nummod 6,GCCCACGCCCC
R1649 T2538 T2529 punct -,5
R1650 T2539 T2529 nummod 3,5
R1651 T2540 T2529 punct ′,5
R1652 T2541 T2514 punct ),oligonucleotides
R1653 T2542 T2497 prep at,inserting
R1654 T2543 T2544 det the,site
R1655 T2544 T2542 pobj site,at
R1656 T2545 T2544 compound BamHI,site
R1657 T2546 T2544 prep of,site
R1658 T2547 T2546 pobj M13mp19,of
R1659 T2548 T2494 cc and,constructed
R1660 T2549 T2550 det the,sequences
R1661 T2550 T2551 nsubjpass sequences,confirmed
R1662 T2551 T2494 conj confirmed,constructed
R1663 T2552 T2550 prep of,sequences
R1664 T2553 T2554 det the,insert
R1665 T2554 T2552 pobj insert,of
R1666 T2555 T2551 auxpass were,confirmed
R1667 T2556 T2551 punct .,confirmed
R1668 T2629 T2630 nmod DNA,helicase
R1669 T2630 T2631 nmod helicase,assays
R1670 T2632 T2630 cc and,helicase
R1671 T2633 T2634 compound gel,shift
R1672 T2634 T2630 conj shift,helicase
R1673 T2636 T2637 prep In,incubated
R1674 T2638 T2639 compound gel,shift
R1675 T2639 T2640 compound shift,assays
R1676 T2640 T2636 pobj assays,In
R1677 T2641 T2637 punct ", ",incubated
R1678 T2642 T2643 nmod Mcm4,proteins
R1679 T2643 T2637 nsubjpass proteins,incubated
R1680 T2644 T2642 punct /,Mcm4
R1681 T2645 T2642 nummod 6,Mcm4
R1682 T2646 T2642 punct /,Mcm4
R1683 T2647 T2642 nummod 7,Mcm4
R1684 T2648 T2637 auxpass were,incubated
R1685 T2649 T2637 prep at,incubated
R1686 T2650 T2651 nummod 30,°C
R1687 T2651 T2649 pobj °C,at
R1688 T2652 T2637 prep for,incubated
R1689 T2653 T2654 nummod 30,min
R1690 T2654 T2652 pobj min,for
R1691 T2655 T2637 prep in,incubated
R1692 T2656 T2657 compound reaction,mixtures
R1693 T2657 T2655 pobj mixtures,in
R1694 T2658 T2659 punct (,μl
R1695 T2659 T2657 parataxis μl,mixtures
R1696 T2660 T2659 nummod 15,μl
R1697 T2661 T2659 punct ),μl
R1698 T2662 T2657 acl containing,mixtures
R1699 T2663 T2664 nummod 25,mM
R1700 T2664 T2665 compound mM,NaOH
R1701 T2665 T2662 dobj NaOH,containing
R1702 T2666 T2665 compound HEPES,NaOH
R1703 T2667 T2665 punct –,NaOH
R1704 T2668 T2669 punct (,pH
R1705 T2669 T2665 parataxis pH,NaOH
R1706 T2670 T2669 nummod 7.5,pH
R1707 T2671 T2669 punct ),pH
R1708 T2672 T2665 punct ", ",NaOH
R1709 T2673 T2674 nummod 50,mM
R1710 T2674 T2675 compound mM,acetate
R1711 T2675 T2665 conj acetate,NaOH
R1712 T2676 T2675 compound sodium,acetate
R1713 T2677 T2675 punct ", ",acetate
R1714 T2678 T2679 nummod 10,mM
R1715 T2679 T2680 nmod mM,CH3COO
R1716 T2680 T2675 conj CH3COO,acetate
R1717 T2681 T2680 nmod Mg,CH3COO
R1718 T2682 T2680 punct (,CH3COO
R1719 T2683 T2680 punct ),CH3COO
R1720 T2684 T2680 nummod 2,CH3COO
R1721 T2685 T2680 punct ", ",CH3COO
R1722 T2686 T2687 nummod 1,mM
R1723 T2687 T2688 compound mM,DTT
R1724 T2688 T2680 conj DTT,CH3COO
R1725 T2689 T2688 punct ", ",DTT
R1726 T2690 T2691 nummod 0.25,μg
R1727 T2691 T2692 nmod μg,BSA
R1728 T2692 T2688 conj BSA,DTT
R1729 T2693 T2694 punct /,ml
R1730 T2694 T2691 prep ml,μg
R1731 T2695 T2692 punct ", ",BSA
R1732 T2696 T2697 nummod 0.5,mM
R1733 T2697 T2698 nmod mM,S
R1734 T2698 T2692 conj S,BSA
R1735 T2699 T2698 nmod ATP,S
R1736 T2700 T2698 punct -,S
R1737 T2701 T2698 punct γ,S
R1738 T2702 T2698 punct -,S
R1739 T2703 T2698 cc and,S
R1740 T2704 T2705 amod labeled,substrates
R1741 T2705 T2698 conj substrates,S
R1742 T2706 T2705 prep of,substrates
R1743 T2707 T2708 det the,amount
R1744 T2708 T2706 pobj amount,of
R1745 T2709 T2708 acl indicated,amount
R1746 T2710 T2637 punct .,incubated
R1747 T2712 T2713 prep After,applied
R1748 T2714 T2712 pobj addition,After
R1749 T2715 T2714 prep of,addition
R1750 T2716 T2717 nummod 2,μl
R1751 T2717 T2715 pobj μl,of
R1752 T2718 T2717 prep of,μl
R1753 T2719 T2720 nummod 50,%
R1754 T2720 T2721 compound %,glycerol
R1755 T2721 T2718 pobj glycerol,of
R1756 T2722 T2713 punct ", ",applied
R1757 T2723 T2724 det the,mixtures
R1758 T2724 T2713 nsubjpass mixtures,applied
R1759 T2725 T2724 compound reaction,mixtures
R1760 T2726 T2713 auxpass were,applied
R1761 T2727 T2713 advmod directly,applied
R1762 T2728 T2713 prep on,applied
R1763 T2729 T2730 det a,gel
R1764 T2730 T2728 pobj gel,on
R1765 T2731 T2730 compound polyacrylamide,gel
R1766 T2732 T2730 acl containing,gel
R1767 T2733 T2734 nummod 6,mM
R1768 T2734 T2735 compound mM,acetate
R1769 T2735 T2732 dobj acetate,containing
R1770 T2736 T2735 compound magnesium,acetate
R1771 T2737 T2735 cc and,acetate
R1772 T2738 T2739 nummod 5,%
R1773 T2739 T2740 compound %,glycerol
R1774 T2740 T2735 conj glycerol,acetate
R1775 T2741 T2732 prep in,containing
R1776 T2742 T2743 nummod 0.5,TBE
R1777 T2743 T2741 pobj TBE,in
R1778 T2744 T2742 punct ×,0.5
R1779 T2745 T2713 punct ", ",applied
R1780 T2746 T2713 cc and,applied
R1781 T2747 T2748 nsubjpass electrophoresis,conducted
R1782 T2748 T2713 conj conducted,applied
R1783 T2749 T2748 auxpass was,conducted
R1784 T2750 T2748 prep at,conducted
R1785 T2751 T2752 compound room,temperature
R1786 T2752 T2750 pobj temperature,at
R1787 T2753 T2748 punct .,conducted
R1788 T2755 T2756 prep For,incubated
R1789 T2757 T2758 compound DNA,assays
R1790 T2758 T2755 pobj assays,For
R1791 T2759 T2758 compound helicase,assays
R1792 T2760 T2756 punct ", ",incubated
R1793 T2761 T2762 det the,mixtures
R1794 T2762 T2756 nsubjpass mixtures,incubated
R1795 T2763 T2762 compound reaction,mixtures
R1796 T2764 T2762 prep of,mixtures
R1797 T2765 T2766 det the,assays
R1798 T2766 T2764 pobj assays,of
R1799 T2767 T2768 compound gel,shift
R1800 T2768 T2766 compound shift,assays
R1801 T2769 T2756 punct ", ",incubated
R1802 T2770 T2771 mark as,described
R1803 T2771 T2756 advcl described,incubated
R1804 T2772 T2771 advmod above,described
R1805 T2773 T2756 punct ", ",incubated
R1806 T2774 T2756 auxpass were,incubated
R1807 T2775 T2756 prep at,incubated
R1808 T2776 T2777 nummod 30,°C
R1809 T2777 T2775 pobj °C,at
R1810 T2778 T2756 prep for,incubated
R1811 T2779 T2780 nummod 15,min
R1812 T2780 T2778 pobj min,for
R1813 T2781 T2756 punct ", ",incubated
R1814 T2782 T2756 cc and,incubated
R1815 T2783 T2782 advmod then,and
R1816 T2784 T2785 det the,ATP
R1817 T2785 T2786 nsubjpass ATP,added
R1818 T2786 T2756 conj added,incubated
R1819 T2787 T2786 auxpass was,added
R1820 T2788 T2786 prep at,added
R1821 T2789 T2790 nummod 10,mM
R1822 T2790 T2788 pobj mM,at
R1823 T2791 T2786 punct ", ",added
R1824 T2792 T2786 advcl followed,added
R1825 T2793 T2792 agent by,followed
R1826 T2794 T2793 pobj incubation,by
R1827 T2795 T2794 prep at,incubation
R1828 T2796 T2797 nummod 37,°C
R1829 T2797 T2795 pobj °C,at
R1830 T2798 T2794 prep for,incubation
R1831 T2799 T2800 amod additional,min
R1832 T2800 T2798 pobj min,for
R1833 T2801 T2800 nummod 30,min
R1834 T2802 T2786 punct .,added
R1835 T2804 T2805 det The,reactions
R1836 T2805 T2806 nsubjpass reactions,terminated
R1837 T2807 T2806 auxpass were,terminated
R1838 T2808 T2806 agent by,terminated
R1839 T2809 T2808 pobj addition,by
R1840 T2810 T2809 prep of,addition
R1841 T2811 T2810 pobj EDTA,of
R1842 T2812 T2813 punct (,mM
R1843 T2813 T2811 parataxis mM,EDTA
R1844 T2814 T2813 nummod 20,mM
R1845 T2815 T2813 punct ),mM
R1846 T2816 T2811 punct ", ",EDTA
R1847 T2817 T2811 conj SDS,EDTA
R1848 T2818 T2819 punct (,%
R1849 T2819 T2817 parataxis %,SDS
R1850 T2820 T2819 nummod 0.1,%
R1851 T2821 T2819 punct ),%
R1852 T2822 T2817 cc and,SDS
R1853 T2823 T2824 nummod 2,μg
R1854 T2824 T2825 compound μg,K
R1855 T2825 T2817 conj K,SDS
R1856 T2826 T2825 compound proteinase,K
R1857 T2827 T2806 punct ", ",terminated
R1858 T2828 T2806 cc and,terminated
R1859 T2829 T2830 auxpass were,incubated
R1860 T2830 T2806 conj incubated,terminated
R1861 T2831 T2830 prep for,incubated
R1862 T2832 T2833 amod additional,min
R1863 T2833 T2831 pobj min,for
R1864 T2834 T2833 nummod 15,min
R1865 T2835 T2806 punct .,terminated
R1866 T2837 T2838 det The,samples
R1867 T2838 T2839 nsubjpass samples,separated
R1868 T2840 T2839 auxpass were,separated
R1869 T2841 T2839 prep by,separated
R1870 T2842 T2841 pobj electrophoresis,by
R1871 T2843 T2839 prep on,separated
R1872 T2844 T2845 amod nondenaturing,gel
R1873 T2845 T2843 pobj gel,on
R1874 T2846 T2845 compound polyacrylamide,gel
R1875 T2847 T2845 prep in,gel
R1876 T2848 T2849 nummod 1,TBE
R1877 T2849 T2847 pobj TBE,in
R1878 T2850 T2848 punct ×,1
R1879 T2851 T2839 punct .,separated
R1880 T2967 T2968 compound Nuclease,protection
R1881 T2968 T2969 compound protection,assays
R1882 T2971 T2972 det The,mixtures
R1883 T2972 T2974 nsubj mixtures,contained
R1884 T2973 T2972 compound reaction,mixtures
R1885 T2975 T2976 punct (,μl
R1886 T2976 T2972 parataxis μl,mixtures
R1887 T2977 T2976 nummod 25,μl
R1888 T2978 T2976 punct ),μl
R1889 T2979 T2980 nummod 25,mM
R1890 T2980 T2981 compound mM,NaOH
R1891 T2981 T2974 dobj NaOH,contained
R1892 T2982 T2981 compound HEPES,NaOH
R1893 T2983 T2981 punct –,NaOH
R1894 T2984 T2985 punct (,pH
R1895 T2985 T2981 parataxis pH,NaOH
R1896 T2986 T2985 nummod 7.5,pH
R1897 T2987 T2985 punct ),pH
R1898 T2988 T2981 punct ", ",NaOH
R1899 T2989 T2990 nummod 50,mM
R1900 T2990 T2991 compound mM,acetate
R1901 T2991 T2981 conj acetate,NaOH
R1902 T2992 T2991 compound sodium,acetate
R1903 T2993 T2991 punct ", ",acetate
R1904 T2994 T2995 nummod 10,mM
R1905 T2995 T2996 nmod mM,CH3COO
R1906 T2996 T2991 conj CH3COO,acetate
R1907 T2997 T2996 nmod Mg,CH3COO
R1908 T2998 T2996 punct (,CH3COO
R1909 T2999 T2996 punct ),CH3COO
R1910 T3000 T2996 nummod 2,CH3COO
R1911 T3001 T2996 punct ", ",CH3COO
R1912 T3002 T3003 nummod 1,mM
R1913 T3003 T3004 compound mM,DTT
R1914 T3004 T2996 conj DTT,CH3COO
R1915 T3005 T3004 punct ", ",DTT
R1916 T3006 T3007 nummod 0.25,mg
R1917 T3007 T3008 nmod mg,BSA
R1918 T3008 T3004 conj BSA,DTT
R1919 T3009 T3010 punct /,ml
R1920 T3010 T3007 prep ml,mg
R1921 T3011 T3008 punct ", ",BSA
R1922 T3012 T3013 nummod 0.5,mM
R1923 T3013 T3014 nmod mM,S
R1924 T3014 T3008 conj S,BSA
R1925 T3015 T3014 nmod ATP,S
R1926 T3016 T3014 punct -,S
R1927 T3017 T3014 punct γ,S
R1928 T3018 T3014 punct -,S
R1929 T3019 T3014 punct ", ",S
R1930 T3020 T3021 nummod 0.5,mM
R1931 T3021 T3022 compound mM,CaCl2
R1932 T3022 T3014 conj CaCl2,S
R1933 T3023 T3022 punct ", ",CaCl2
R1934 T3024 T3025 nummod 10,fmol
R1935 T3025 T3022 conj fmol,CaCl2
R1936 T3026 T3025 prep of,fmol
R1937 T3027 T3028 npadvmod 32P,labeled
R1938 T3028 T3030 amod labeled,substrate
R1939 T3029 T3028 punct -,labeled
R1940 T3030 T3026 pobj substrate,of
R1941 T3031 T3030 compound bubble,substrate
R1942 T3032 T3025 punct ", ",fmol
R1943 T3033 T3025 cc and,fmol
R1944 T3034 T3035 det the,amount
R1945 T3035 T3025 conj amount,fmol
R1946 T3036 T3035 amod indicated,amount
R1947 T3037 T3035 prep of,amount
R1948 T3038 T3039 nmod Mcm4,proteins
R1949 T3039 T3037 pobj proteins,of
R1950 T3040 T3038 punct /,Mcm4
R1951 T3041 T3038 nummod 6,Mcm4
R1952 T3042 T3038 punct /,Mcm4
R1953 T3043 T3038 nummod 7,Mcm4
R1954 T3044 T2974 punct .,contained
R1955 T3046 T3047 prep After,added
R1956 T3048 T3046 pobj incubation,After
R1957 T3049 T3048 prep at,incubation
R1958 T3050 T3051 nummod 30,°C
R1959 T3051 T3049 pobj °C,at
R1960 T3052 T3048 prep for,incubation
R1961 T3053 T3054 nummod 30,min
R1962 T3054 T3052 pobj min,for
R1963 T3055 T3047 punct ", ",added
R1964 T3056 T3057 det the,amount
R1965 T3057 T3047 nsubjpass amount,added
R1966 T3058 T3057 amod indicated,amount
R1967 T3059 T3057 prep of,amount
R1968 T3060 T3059 pobj DNase,of
R1969 T3061 T3060 nummod I,DNase
R1970 T3062 T3063 punct (,Biomedical
R1971 T3063 T3060 parataxis Biomedical,DNase
R1972 T3064 T3063 compound TAKARA,Biomedical
R1973 T3065 T3063 punct ", ",Biomedical
R1974 T3066 T3063 npadvmod Japan,Biomedical
R1975 T3067 T3063 punct ),Biomedical
R1976 T3068 T3047 auxpass was,added
R1977 T3069 T3047 punct ", ",added
R1978 T3070 T3047 cc and,added
R1979 T3071 T3072 det the,mixtures
R1980 T3072 T3073 nsubjpass mixtures,incubated
R1981 T3073 T3047 conj incubated,added
R1982 T3074 T3073 auxpass were,incubated
R1983 T3075 T3073 prep at,incubated
R1984 T3076 T3077 compound room,temperature
R1985 T3077 T3075 pobj temperature,at
R1986 T3078 T3073 prep for,incubated
R1987 T3079 T3080 nummod 1,min
R1988 T3080 T3078 pobj min,for
R1989 T3081 T3047 punct .,added
R1990 T3083 T3084 nsubjpass DNase,inactivated
R1991 T3085 T3083 nummod I,DNase
R1992 T3086 T3084 auxpass was,inactivated
R1993 T3087 T3084 agent by,inactivated
R1994 T3088 T3089 det the,addition
R1995 T3089 T3087 pobj addition,by
R1996 T3090 T3089 prep of,addition
R1997 T3091 T3092 nummod 250,μl
R1998 T3092 T3090 pobj μl,of
R1999 T3093 T3092 prep of,μl
R2000 T3094 T3095 compound stop,solution
R2001 T3095 T3093 pobj solution,of
R2002 T3096 T3097 punct (,EDTA
R2003 T3097 T3084 parataxis EDTA,inactivated
R2004 T3098 T3099 nummod 20,mM
R2005 T3099 T3097 compound mM,EDTA
R2006 T3100 T3097 punct ", ",EDTA
R2007 T3101 T3102 compound 0.2M,chloride
R2008 T3102 T3097 conj chloride,EDTA
R2009 T3103 T3102 compound sodium,chloride
R2010 T3104 T3102 punct ", ",chloride
R2011 T3105 T3106 nummod 1,%
R2012 T3106 T3107 compound %,SDS
R2013 T3107 T3102 conj SDS,chloride
R2014 T3108 T3107 cc and,SDS
R2015 T3109 T3110 nummod 12.5,μg
R2016 T3110 T3111 nmod μg,RNA
R2017 T3111 T3107 conj RNA,SDS
R2018 T3112 T3113 punct /,ml
R2019 T3113 T3110 prep ml,μg
R2020 T3114 T3111 compound yeast,RNA
R2021 T3115 T3111 punct ),RNA
R2022 T3116 T3084 punct .,inactivated
R2023 T3118 T3119 prep For,carried
R2024 T3120 T3121 compound nuclease,footprinting
R2025 T3121 T3123 compound footprinting,assay
R2026 T3122 T3121 compound P1,footprinting
R2027 T3123 T3118 pobj assay,For
R2028 T3124 T3119 punct ", ",carried
R2029 T3125 T3126 compound binding,reactions
R2030 T3126 T3119 nsubjpass reactions,carried
R2031 T3127 T3119 auxpass were,carried
R2032 T3128 T3119 prt out,carried
R2033 T3129 T3119 prep under,carried
R2034 T3130 T3131 det the,conditions
R2035 T3131 T3129 pobj conditions,under
R2036 T3132 T3131 amod same,conditions
R2037 T3133 T3131 prep except,conditions
R2038 T3134 T3135 mark that,omitted
R2039 T3135 T3133 pcomp omitted,except
R2040 T3136 T3135 nsubjpass CaCl2,omitted
R2041 T3137 T3135 auxpass was,omitted
R2042 T3138 T3119 punct .,carried
R2043 T3140 T3141 compound P1,nuclease
R2044 T3141 T3142 nsubjpass nuclease,added
R2045 T3143 T3144 punct (,Roche
R2046 T3144 T3141 parataxis Roche,nuclease
R2047 T3145 T3146 nummod 0.3,U
R2048 T3146 T3144 dep U,Roche
R2049 T3147 T3144 punct ", ",Roche
R2050 T3148 T3144 punct ),Roche
R2051 T3149 T3142 auxpass was,added
R2052 T3150 T3142 cc and,added
R2053 T3151 T3152 nsubjpass incubation,continued
R2054 T3152 T3142 conj continued,added
R2055 T3153 T3152 auxpass was,continued
R2056 T3154 T3152 prep at,continued
R2057 T3155 T3156 nummod 37,°C
R2058 T3156 T3154 pobj °C,at
R2059 T3157 T3152 prep for,continued
R2060 T3158 T3159 nummod 1,min
R2061 T3159 T3157 pobj min,for
R2062 T3160 T3152 punct .,continued
R2063 T3162 T3163 nsubjpass Cleavage,stopped
R2064 T3163 T3165 ccomp stopped,incubated
R2065 T3164 T3163 auxpass was,stopped
R2066 T3166 T3163 prep by,stopped
R2067 T3167 T3166 pobj addition,by
R2068 T3168 T3167 prep of,addition
R2069 T3169 T3170 nummod 50,μl
R2070 T3170 T3168 pobj μl,of
R2071 T3171 T3170 prep of,μl
R2072 T3172 T3173 nummod 25,mM
R2073 T3173 T3174 compound mM,EDTA
R2074 T3174 T3171 pobj EDTA,of
R2075 T3175 T3174 cc and,EDTA
R2076 T3176 T3177 nummod 0.4,M
R2077 T3177 T3178 compound M,NaOH
R2078 T3178 T3174 conj NaOH,EDTA
R2079 T3179 T3165 punct ;,incubated
R2080 T3180 T3181 det the,mixture
R2081 T3181 T3165 nsubjpass mixture,incubated
R2082 T3182 T3165 auxpass was,incubated
R2083 T3183 T3165 prep at,incubated
R2084 T3184 T3185 compound room,temperature
R2085 T3185 T3183 pobj temperature,at
R2086 T3186 T3165 prep for,incubated
R2087 T3187 T3188 nummod 10,min
R2088 T3188 T3186 pobj min,for
R2089 T3189 T3188 acl followed,min
R2090 T3190 T3189 agent by,followed
R2091 T3191 T3192 det the,addition
R2092 T3192 T3190 pobj addition,by
R2093 T3193 T3192 prep of,addition
R2094 T3194 T3195 nummod 12,μl
R2095 T3195 T3193 pobj μl,of
R2096 T3196 T3195 prep of,μl
R2097 T3197 T3198 nummod 3,M
R2098 T3198 T3199 compound M,acetate
R2099 T3199 T3196 pobj acetate,of
R2100 T3200 T3199 compound sodium,acetate
R2101 T3201 T3202 punct (,pH
R2102 T3202 T3199 parataxis pH,acetate
R2103 T3203 T3202 nummod 4.8,pH
R2104 T3204 T3202 punct ),pH
R2105 T3205 T3165 punct .,incubated
R2106 T3207 T3208 nsubjpass Proteins,removed
R2107 T3209 T3208 auxpass were,removed
R2108 T3210 T3208 prep by,removed
R2109 T3211 T3212 compound phenol,chloroform
R2110 T3212 T3214 compound chloroform,extraction
R2111 T3213 T3212 punct –,chloroform
R2112 T3214 T3210 pobj extraction,by
R2113 T3215 T3208 punct ", ",removed
R2114 T3216 T3208 cc and,removed
R2115 T3217 T3218 nsubjpass DNAs,collected
R2116 T3218 T3208 conj collected,removed
R2117 T3219 T3218 auxpass were,collected
R2118 T3220 T3218 prep by,collected
R2119 T3221 T3222 compound ethanol,precipitation
R2120 T3222 T3220 pobj precipitation,by
R2121 T3223 T3222 acl followed,precipitation
R2122 T3224 T3223 agent by,followed
R2123 T3225 T3224 pobj wash,by
R2124 T3226 T3225 prep with,wash
R2125 T3227 T3228 nummod 70,%
R2126 T3228 T3229 compound %,ethanol
R2127 T3229 T3226 pobj ethanol,with
R2128 T3230 T3218 punct .,collected
R2129 T3232 T3233 det The,DNA
R2130 T3233 T3235 nsubjpass DNA,subjected
R2131 T3234 T3233 amod digested,DNA
R2132 T3236 T3235 auxpass was,subjected
R2133 T3237 T3235 prep to,subjected
R2134 T3238 T3237 pobj electrophoresis,to
R2135 T3239 T3238 prep through,electrophoresis
R2136 T3240 T3241 det a,gel
R2137 T3241 T3239 pobj gel,through
R2138 T3242 T3243 nummod 10,%
R2139 T3243 T3241 compound %,gel
R2140 T3244 T3242 cc or,10
R2141 T3245 T3242 conj 12,10
R2142 T3246 T3241 compound polyacrylamide,gel
R2143 T3247 T3241 compound sequencing,gel
R2144 T3248 T3241 acl containing,gel
R2145 T3249 T3250 nummod 7,M
R2146 T3250 T3251 compound M,urea
R2147 T3251 T3248 dobj urea,containing
R2148 T3252 T3238 punct ", ",electrophoresis
R2149 T3253 T3238 acl followed,electrophoresis
R2150 T3254 T3253 agent by,followed
R2151 T3255 T3254 pobj autoradiography,by
R2152 T3256 T3235 punct .,subjected
R2153 T3428 T3429 nmod Mcm4,complex
R2154 T3429 T3434 nsubj complex,interacts
R2155 T3430 T3428 punct /,Mcm4
R2156 T3431 T3428 nummod 6,Mcm4
R2157 T3432 T3428 punct /,Mcm4
R2158 T3433 T3428 nummod 7,Mcm4
R2159 T3435 T3434 advmod primarily,interacts
R2160 T3436 T3434 prep with,interacts
R2161 T3437 T3438 det the,segment
R2162 T3438 T3436 pobj segment,with
R2163 T3439 T3440 amod single,stranded
R2164 T3440 T3438 amod stranded,segment
R2165 T3441 T3440 punct -,stranded
R2166 T3442 T3438 compound DNA,segment
R2167 T3444 T3445 nsubj Mcm4,binds
R2168 T3446 T3444 punct /,Mcm4
R2169 T3447 T3444 nummod 6,Mcm4
R2170 T3448 T3444 punct /,Mcm4
R2171 T3449 T3444 nummod 7,Mcm4
R2172 T3450 T3445 prep to,binds
R2173 T3451 T3452 punct ‘,substrates
R2174 T3452 T3450 pobj substrates,to
R2175 T3453 T3452 nmod bubble,substrates
R2176 T3454 T3452 punct ’,substrates
R2177 T3455 T3452 compound DNA,substrates
R2178 T3456 T3445 cc and,binds
R2179 T3457 T3445 conj unwinds,binds
R2180 T3458 T3459 det the,segments
R2181 T3459 T3457 dobj segments,unwinds
R2182 T3460 T3459 compound duplex,segments
R2183 T3461 T3462 advmod when,contains
R2184 T3462 T3457 advcl contains,unwinds
R2185 T3463 T3464 det the,segment
R2186 T3464 T3462 nsubj segment,contains
R2187 T3465 T3464 amod central,segment
R2188 T3466 T3467 amod single,stranded
R2189 T3467 T3464 amod stranded,segment
R2190 T3468 T3467 punct -,stranded
R2191 T3469 T3470 compound thymine,stretches
R2192 T3470 T3462 dobj stretches,contains
R2193 T3471 T3472 punct (,13
R2194 T3472 T3462 parataxis 13,contains
R2195 T3473 T3472 punct ),13
R2196 T3474 T3445 punct .,binds
R2197 T3476 T3477 prep In,conducted
R2198 T3478 T3476 pobj order,In
R2199 T3479 T3480 aux to,examine
R2200 T3480 T3478 acl examine,order
R2201 T3481 T3482 det the,mode
R2202 T3482 T3480 dobj mode,examine
R2203 T3483 T3482 prep of,mode
R2204 T3484 T3483 pobj binding,of
R2205 T3485 T3484 prep of,binding
R2206 T3486 T3485 pobj Mcm4,of
R2207 T3487 T3486 punct /,Mcm4
R2208 T3488 T3486 nummod 6,Mcm4
R2209 T3489 T3486 punct /,Mcm4
R2210 T3490 T3486 nummod 7,Mcm4
R2211 T3491 T3484 prep on,binding
R2212 T3492 T3493 compound bubble,DNAs
R2213 T3493 T3491 pobj DNAs,on
R2214 T3494 T3477 punct ", ",conducted
R2215 T3495 T3477 nsubj we,conducted
R2216 T3496 T3497 compound nuclease,assays
R2217 T3497 T3477 dobj assays,conducted
R2218 T3498 T3497 compound protection,assays
R2219 T3499 T3477 advcl using,conducted
R2220 T3500 T3501 amod labeled,DNAs
R2221 T3501 T3499 dobj DNAs,using
R2222 T3502 T3503 compound bubble,substrate
R2223 T3503 T3501 compound substrate,DNAs
R2224 T3504 T3501 cc and,DNAs
R2225 T3505 T3501 conj Mcm4,DNAs
R2226 T3506 T3505 punct /,Mcm4
R2227 T3507 T3505 nummod 6,Mcm4
R2228 T3508 T3505 punct /,Mcm4
R2229 T3509 T3505 nummod 7,Mcm4
R2230 T3510 T3477 prep in,conducted
R2231 T3511 T3512 det the,presence
R2232 T3512 T3510 pobj presence,in
R2233 T3513 T3512 prep of,presence
R2234 T3514 T3515 nmod ATP,S
R2235 T3515 T3513 pobj S,of
R2236 T3516 T3515 punct -,S
R2237 T3517 T3515 punct γ,S
R2238 T3518 T3515 punct -,S
R2239 T3519 T3520 dep which,permits
R2240 T3520 T3515 relcl permits,S
R2241 T3521 T3520 dobj binding,permits
R2242 T3522 T3521 prep to,binding
R2243 T3523 T3524 compound substrate,DNA
R2244 T3524 T3522 pobj DNA,to
R2245 T3525 T3520 cc but,permits
R2246 T3526 T3527 aux does,mobilize
R2247 T3527 T3520 conj mobilize,permits
R2248 T3528 T3527 neg not,mobilize
R2249 T3529 T3530 det the,helicase
R2250 T3530 T3527 dobj helicase,mobilize
R2251 T3531 T3477 punct .,conducted
R2252 T3533 T3534 prep At,detected
R2253 T3535 T3536 det an,concentration
R2254 T3536 T3533 pobj concentration,At
R2255 T3537 T3536 amod optimum,concentration
R2256 T3538 T3536 prep of,concentration
R2257 T3539 T3538 pobj DNase,of
R2258 T3540 T3539 nummod I,DNase
R2259 T3541 T3534 punct ", ",detected
R2260 T3542 T3543 amod strong,cleavages
R2261 T3543 T3534 nsubjpass cleavages,detected
R2262 T3544 T3534 auxpass were,detected
R2263 T3545 T3534 prep on,detected
R2264 T3546 T3547 det the,regions
R2265 T3547 T3545 pobj regions,on
R2266 T3548 T3549 nummod 21,bp
R2267 T3549 T3547 compound bp,regions
R2268 T3550 T3547 compound duplex,regions
R2269 T3551 T3534 prep at,detected
R2270 T3552 T3553 det both,ends
R2271 T3553 T3551 pobj ends,at
R2272 T3554 T3553 prep of,ends
R2273 T3555 T3556 det the,substrates
R2274 T3556 T3554 pobj substrates,of
R2275 T3557 T3534 punct ", ",detected
R2276 T3558 T3559 mark whereas,detected
R2277 T3559 T3534 advcl detected,detected
R2278 T3560 T3561 amod weaker,cleavages
R2279 T3561 T3559 nsubjpass cleavages,detected
R2280 T3562 T3559 auxpass were,detected
R2281 T3563 T3559 prep on,detected
R2282 T3564 T3565 det the,region
R2283 T3565 T3563 pobj region,on
R2284 T3566 T3565 amod central,region
R2285 T3567 T3568 amod single,stranded
R2286 T3568 T3565 amod stranded,region
R2287 T3569 T3568 punct -,stranded
R2288 T3570 T3559 punct ", ",detected
R2289 T3571 T3559 advcl consistent,detected
R2290 T3572 T3571 prep with,consistent
R2291 T3573 T3572 pobj preference,with
R2292 T3574 T3573 prep of,preference
R2293 T3575 T3574 pobj DNase,of
R2294 T3576 T3575 nummod I,DNase
R2295 T3577 T3573 prep to,preference
R2296 T3578 T3579 amod double,stranded
R2297 T3579 T3581 amod stranded,DNA
R2298 T3580 T3579 punct -,stranded
R2299 T3581 T3577 pobj DNA,to
R2300 T3582 T3534 punct .,detected
R2301 T3584 T3585 prep With,detected
R2302 T3586 T3587 det the,substrate
R2303 T3587 T3584 pobj substrate,With
R2304 T3588 T3587 nmod Bub66,substrate
R2305 T3589 T3588 punct /,Bub66
R2306 T3590 T3588 appos T,Bub66
R2307 T3591 T3590 punct -,T
R2308 T3592 T3590 amod rich,T
R2309 T3593 T3585 punct ", ",detected
R2310 T3594 T3595 amod strong,protection
R2311 T3595 T3585 nsubjpass protection,detected
R2312 T3596 T3585 auxpass was,detected
R2313 T3597 T3585 prep on,detected
R2314 T3598 T3599 det the,regions
R2315 T3599 T3597 pobj regions,on
R2316 T3600 T3599 amod entire,regions
R2317 T3601 T3602 amod single,stranded
R2318 T3602 T3599 amod stranded,regions
R2319 T3603 T3602 punct -,stranded
R2320 T3604 T3599 prep of,regions
R2321 T3605 T3606 preconj both,top
R2322 T3606 T3607 amod top,strands
R2323 T3607 T3604 pobj strands,of
R2324 T3608 T3606 cc and,top
R2325 T3609 T3606 conj bottom,top
R2326 T3610 T3585 prep with,detected
R2327 T3611 T3612 amod increasing,concentration
R2328 T3612 T3610 pobj concentration,with
R2329 T3613 T3612 prep of,concentration
R2330 T3614 T3613 pobj Mcm4,of
R2331 T3615 T3614 punct /,Mcm4
R2332 T3616 T3614 nummod 6,Mcm4
R2333 T3617 T3614 punct /,Mcm4
R2334 T3618 T3614 nummod 7,Mcm4
R2335 T3619 T3585 punct ", ",detected
R2336 T3620 T3585 advcl consistent,detected
R2337 T3621 T3620 prep with,consistent
R2338 T3622 T3623 amod high,affinity
R2339 T3623 T3621 pobj affinity,with
R2340 T3624 T3623 prep of,affinity
R2341 T3625 T3624 pobj Mcm4,of
R2342 T3626 T3625 punct /,Mcm4
R2343 T3627 T3625 nummod 6,Mcm4
R2344 T3628 T3625 punct /,Mcm4
R2345 T3629 T3625 nummod 7,Mcm4
R2346 T3630 T3623 prep to,affinity
R2347 T3631 T3632 nmod T,sequences
R2348 T3632 T3630 pobj sequences,to
R2349 T3633 T3631 punct -,T
R2350 T3634 T3631 amod rich,T
R2351 T3635 T3632 compound bubble,sequences
R2352 T3636 T3637 punct (,1A
R2353 T3637 T3585 parataxis 1A,detected
R2354 T3638 T3637 compound Figure,1A
R2355 T3639 T3637 punct ),1A
R2356 T3640 T3585 punct .,detected
R2357 T3642 T3643 amod Similar,protection
R2358 T3643 T3648 nsubjpass protection,detected
R2359 T3644 T3642 punct ", ",Similar
R2360 T3645 T3642 cc but,Similar
R2361 T3646 T3647 advmod less,significant
R2362 T3647 T3642 conj significant,Similar
R2363 T3649 T3648 auxpass was,detected
R2364 T3650 T3648 prep on,detected
R2365 T3651 T3652 det the,segment
R2366 T3652 T3650 pobj segment,on
R2367 T3653 T3654 amod single,stranded
R2368 T3654 T3652 amod stranded,segment
R2369 T3655 T3654 punct -,stranded
R2370 T3656 T3652 compound DNA,segment
R2371 T3657 T3652 prep with,segment
R2372 T3658 T3659 compound P1,nucleases
R2373 T3659 T3657 pobj nucleases,with
R2374 T3660 T3661 punct (,1A
R2375 T3661 T3652 parataxis 1A,segment
R2376 T3662 T3661 compound Figure,1A
R2377 T3663 T3661 punct ),1A
R2378 T3664 T3652 punct ", ",segment
R2379 T3665 T3666 dep which,is
R2380 T3666 T3652 relcl is,segment
R2381 T3667 T3666 acomp specific,is
R2382 T3668 T3667 prep to,specific
R2383 T3669 T3670 amod single,stranded
R2384 T3670 T3672 amod stranded,DNA
R2385 T3671 T3670 punct -,stranded
R2386 T3672 T3668 pobj DNA,to
R2387 T3673 T3648 punct .,detected
R2388 T3675 T3676 det The,results
R2389 T3676 T3677 nsubj results,indicate
R2390 T3678 T3679 mark that,loaded
R2391 T3679 T3677 ccomp loaded,indicate
R2392 T3680 T3681 det the,complex
R2393 T3681 T3679 nsubjpass complex,loaded
R2394 T3682 T3681 compound Mcm,complex
R2395 T3683 T3679 auxpass is,loaded
R2396 T3684 T3679 prep onto,loaded
R2397 T3685 T3686 compound bubble,DNA
R2398 T3686 T3684 pobj DNA,onto
R2399 T3687 T3679 prep through,loaded
R2400 T3688 T3687 pobj binding,through
R2401 T3689 T3688 prep to,binding
R2402 T3690 T3691 det both,strands
R2403 T3691 T3689 pobj strands,to
R2404 T3692 T3691 prep of,strands
R2405 T3693 T3694 det the,region
R2406 T3694 T3692 pobj region,of
R2407 T3695 T3696 amod single,stranded
R2408 T3696 T3694 amod stranded,region
R2409 T3697 T3696 punct -,stranded
R2410 T3698 T3677 punct .,indicate
R2411 T3700 T3701 amod Careful,examination
R2412 T3701 T3702 nsubj examination,indicated
R2413 T3703 T3701 prep of,examination
R2414 T3704 T3705 nmod Mcm4,footprints
R2415 T3705 T3703 pobj footprints,of
R2416 T3706 T3704 punct /,Mcm4
R2417 T3707 T3704 nummod 6,Mcm4
R2418 T3708 T3704 punct /,Mcm4
R2419 T3709 T3704 nummod 7,Mcm4
R2420 T3710 T3705 prep on,footprints
R2421 T3711 T3712 det a,bubble
R2422 T3712 T3710 pobj bubble,on
R2423 T3713 T3714 amod strong,protection
R2424 T3714 T3702 dobj protection,indicated
R2425 T3715 T3714 prep on,protection
R2426 T3716 T3717 det the,half
R2427 T3717 T3715 pobj half,on
R2428 T3718 T3717 nummod 5,half
R2429 T3719 T3718 punct ′,5
R2430 T3720 T3717 punct -,half
R2431 T3721 T3717 prep of,half
R2432 T3722 T3723 det the,strand
R2433 T3723 T3721 pobj strand,of
R2434 T3724 T3723 amod top,strand
R2435 T3725 T3714 cc and,protection
R2436 T3726 T3727 amod moderate,protection
R2437 T3727 T3714 conj protection,protection
R2438 T3728 T3727 prep on,protection
R2439 T3729 T3730 det the,half
R2440 T3730 T3728 pobj half,on
R2441 T3731 T3730 amod remaining,half
R2442 T3732 T3730 nummod 3,half
R2443 T3733 T3732 punct ′,3
R2444 T3734 T3730 punct -,half
R2445 T3735 T3728 punct ", ",on
R2446 T3736 T3737 advmod as,as
R2447 T3737 T3728 cc as,on
R2448 T3738 T3737 advmod well,as
R2449 T3739 T3728 conj on,on
R2450 T3740 T3741 amod several,segment
R2451 T3741 T3739 pobj segment,on
R2452 T3742 T3743 compound base,pair
R2453 T3743 T3741 compound pair,segment
R2454 T3744 T3743 punct -,pair
R2455 T3745 T3741 compound duplex,segment
R2456 T3746 T3741 amod adjacent,segment
R2457 T3747 T3746 prep to,adjacent
R2458 T3748 T3749 det the,point
R2459 T3749 T3747 pobj point,to
R2460 T3750 T3749 compound branch,point
R2461 T3751 T3752 punct (,1A
R2462 T3752 T3739 parataxis 1A,on
R2463 T3753 T3752 compound Figure,1A
R2464 T3754 T3752 cc and,1A
R2465 T3755 T3752 conj data,1A
R2466 T3756 T3757 neg not,shown
R2467 T3757 T3755 acl shown,data
R2468 T3758 T3752 punct ),1A
R2469 T3759 T3702 punct .,indicated
R2470 T3761 T3762 det A,pattern
R2471 T3762 T3764 nsubjpass pattern,observed
R2472 T3763 T3762 amod similar,pattern
R2473 T3765 T3762 prep of,pattern
R2474 T3766 T3765 pobj protection,of
R2475 T3767 T3764 auxpass was,observed
R2476 T3768 T3764 advmod also,observed
R2477 T3769 T3764 prep on,observed
R2478 T3770 T3771 det the,strand
R2479 T3771 T3769 pobj strand,on
R2480 T3772 T3771 amod bottom,strand
R2481 T3773 T3764 punct .,observed
R2482 T3775 T3776 nsubj This,suggests
R2483 T3777 T3778 mark that,bind
R2484 T3778 T3776 ccomp bind,suggests
R2485 T3779 T3778 nsubj Mcm4,bind
R2486 T3780 T3779 punct /,Mcm4
R2487 T3781 T3779 nummod 6,Mcm4
R2488 T3782 T3779 punct /,Mcm4
R2489 T3783 T3779 nummod 7,Mcm4
R2490 T3784 T3778 aux may,bind
R2491 T3785 T3778 prep to,bind
R2492 T3786 T3787 det a,substrate
R2493 T3787 T3785 pobj substrate,to
R2494 T3788 T3787 compound replication,substrate
R2495 T3789 T3787 compound bubble,substrate
R2496 T3790 T3787 prep with,substrate
R2497 T3791 T3792 det a,symmetry
R2498 T3792 T3790 pobj symmetry,with
R2499 T3793 T3792 nummod 2-fold,symmetry
R2500 T3794 T3778 prep as,bind
R2501 T3795 T3796 det a,hexamer
R2502 T3796 T3794 pobj hexamer,as
R2503 T3797 T3796 amod double,hexamer
R2504 T3798 T3799 punct (,1A
R2505 T3799 T3796 parataxis 1A,hexamer
R2506 T3800 T3799 compound Figure,1A
R2507 T3801 T3799 punct ", ",1A
R2508 T3802 T3799 advcl see,1A
R2509 T3803 T3804 det the,scheme
R2510 T3804 T3802 dobj scheme,see
R2511 T3805 T3804 amod lower,scheme
R2512 T3806 T3799 punct ),1A
R2513 T3807 T3778 punct ", ",bind
R2514 T3808 T3778 prep in,bind
R2515 T3809 T3810 det a,manner
R2516 T3810 T3808 pobj manner,in
R2517 T3811 T3810 amod similar,manner
R2518 T3812 T3811 prep to,similar
R2519 T3813 T3814 compound SV40,antigen
R2520 T3814 T3812 pobj antigen,to
R2521 T3815 T3814 compound T,antigen
R2522 T3816 T3814 punct -,antigen
R2523 T3817 T3818 punct (,21
R2524 T3818 T3778 parataxis 21,bind
R2525 T3819 T3818 punct ),21
R2526 T3820 T3776 punct .,suggests
R2527 T3822 T3823 compound Nuclease,protection
R2528 T3823 T3824 compound protection,assays
R2529 T3824 T3825 nsubjpass assays,conducted
R2530 T3826 T3825 auxpass were,conducted
R2531 T3827 T3825 advmod also,conducted
R2532 T3828 T3825 prep on,conducted
R2533 T3829 T3830 det a,structure
R2534 T3830 T3828 pobj structure,on
R2535 T3831 T3832 npadvmod T,tailed
R2536 T3832 T3830 amod tailed,structure
R2537 T3833 T3832 punct -,tailed
R2538 T3834 T3835 compound Y,fork
R2539 T3835 T3830 compound fork,structure
R2540 T3836 T3835 punct -,fork
R2541 T3837 T3838 punct (,1B
R2542 T3838 T3825 parataxis 1B,conducted
R2543 T3839 T3838 compound Figure,1B
R2544 T3840 T3838 punct ),1B
R2545 T3841 T3825 punct .,conducted
R2546 T3843 T3844 preconj Not,region
R2547 T3844 T3851 nsubjpass region,protected
R2548 T3845 T3843 advmod only,Not
R2549 T3846 T3844 det the,region
R2550 T3847 T3848 amod single,stranded
R2551 T3848 T3844 amod stranded,region
R2552 T3849 T3848 punct -,stranded
R2553 T3850 T3844 compound tail,region
R2554 T3852 T3844 cc but,region
R2555 T3853 T3852 advmod also,but
R2556 T3854 T3855 det the,DNA
R2557 T3855 T3844 conj DNA,region
R2558 T3856 T3857 nummod 7,bp
R2559 T3857 T3855 compound bp,DNA
R2560 T3858 T3855 compound duplex,DNA
R2561 T3859 T3855 amod adjacent,DNA
R2562 T3860 T3859 prep to,adjacent
R2563 T3861 T3862 det the,junction
R2564 T3862 T3860 pobj junction,to
R2565 T3863 T3862 nmod duplex,junction
R2566 T3864 T3863 punct -,duplex
R2567 T3865 T3863 prep to,duplex
R2568 T3866 T3865 punct -,to
R2569 T3867 T3868 amod single,strand
R2570 T3868 T3865 pobj strand,to
R2571 T3869 T3868 punct -,strand
R2572 T3870 T3851 auxpass were,protected
R2573 T3871 T3851 agent by,protected
R2574 T3872 T3873 compound Mcm,binding
R2575 T3873 T3871 pobj binding,by
R2576 T3874 T3873 prep from,binding
R2577 T3875 T3876 compound DNaseI,digestion
R2578 T3876 T3874 pobj digestion,from
R2579 T3877 T3851 punct .,protected
R2580 T3879 T3880 det These,results
R2581 T3880 T3881 nsubj results,indicate
R2582 T3882 T3883 mark that,interacts
R2583 T3883 T3881 advcl interacts,indicate
R2584 T3884 T3885 nmod Mcm4,protein
R2585 T3885 T3883 nsubj protein,interacts
R2586 T3886 T3884 punct /,Mcm4
R2587 T3887 T3884 nummod 6,Mcm4
R2588 T3888 T3884 punct /,Mcm4
R2589 T3889 T3884 nummod 7,Mcm4
R2590 T3890 T3883 advmod primarily,interacts
R2591 T3891 T3883 prep with,interacts
R2592 T3892 T3893 amod single,stranded
R2593 T3893 T3895 amod stranded,region
R2594 T3894 T3893 punct -,stranded
R2595 T3895 T3891 pobj region,with
R2596 T3896 T3895 compound DNA,region
R2597 T3897 T3883 cc and,interacts
R2598 T3898 T3899 mark that,spans
R2599 T3899 T3883 conj spans,interacts
R2600 T3900 T3901 det the,interaction
R2601 T3901 T3899 nsubj interaction,spans
R2602 T3902 T3899 advmod also,spans
R2603 T3903 T3904 det the,segments
R2604 T3904 T3899 dobj segments,spans
R2605 T3905 T3904 compound duplex,segments
R2606 T3906 T3904 prep near,segments
R2607 T3907 T3908 det the,branchpoint
R2608 T3908 T3906 pobj branchpoint,near
R2609 T3909 T3908 prep of,branchpoint
R2610 T3910 T3911 det the,fork
R2611 T3911 T3909 pobj fork,of
R2612 T3912 T3911 compound replication,fork
R2613 T3913 T3881 punct .,indicate
R2617 T4077 T4078 nsubj Formation,facilitates
R2618 T4079 T4077 prep of,Formation
R2619 T4080 T4081 amod double,hexameric
R2620 T4081 T4082 amod hexameric,complexes
R2621 T4082 T4079 pobj complexes,of
R2622 T4083 T4082 nmod Mcm4,complexes
R2623 T4084 T4083 punct /,Mcm4
R2624 T4085 T4083 nummod 6,Mcm4
R2625 T4086 T4083 punct /,Mcm4
R2626 T4087 T4083 nummod 7,Mcm4
R2627 T4088 T4089 compound helicase,actions
R2628 T4089 T4078 dobj actions,facilitates
R2629 T4090 T4078 prep on,facilitates
R2630 T4091 T4092 compound bubble,substrates
R2631 T4092 T4090 pobj substrates,on
R2632 T4094 T4095 det The,results
R2633 T4095 T4097 nsubj results,suggest
R2634 T4096 T4095 amod above,results
R2635 T4098 T4099 mark that,generates
R2636 T4099 T4097 ccomp generates,suggest
R2637 T4100 T4099 nsubj Mcm4,generates
R2638 T4101 T4100 punct /,Mcm4
R2639 T4102 T4100 nummod 6,Mcm4
R2640 T4103 T4100 punct /,Mcm4
R2641 T4104 T4100 nummod 7,Mcm4
R2642 T4105 T4106 det a,complex
R2643 T4106 T4099 dobj complex,generates
R2644 T4107 T4108 amod double,hexameric
R2645 T4108 T4106 amod hexameric,complex
R2646 T4109 T4099 prep on,generates
R2647 T4110 T4111 det a,bubble
R2648 T4111 T4109 pobj bubble,on
R2649 T4112 T4099 punct ", ",generates
R2650 T4113 T4114 det each,hexamer
R2651 T4114 T4115 dep hexamer,makes
R2652 T4115 T4099 ccomp makes,generates
R2653 T4116 T4114 prep of,hexamer
R2654 T4117 T4116 pobj which,of
R2655 T4118 T4119 amod symmetric,contact
R2656 T4119 T4115 dobj contact,makes
R2657 T4120 T4119 prep with,contact
R2658 T4121 T4122 det the,segment
R2659 T4122 T4120 pobj segment,with
R2660 T4123 T4124 npadvmod 5,proximal
R2661 T4124 T4122 amod proximal,segment
R2662 T4125 T4123 punct ′,5
R2663 T4126 T4124 punct -,proximal
R2664 T4127 T4122 amod central,segment
R2665 T4128 T4129 amod single,stranded
R2666 T4129 T4122 amod stranded,segment
R2667 T4130 T4129 punct -,stranded
R2668 T4131 T4097 punct .,suggest
R2669 T4133 T4134 nsubj This,predicts
R2670 T4135 T4136 mark that,needs
R2671 T4136 T4134 ccomp needs,predicts
R2672 T4137 T4138 det the,segment
R2673 T4138 T4136 nsubj segment,needs
R2674 T4139 T4140 amod single,stranded
R2675 T4140 T4138 amod stranded,segment
R2676 T4141 T4140 punct -,stranded
R2677 T4142 T4143 aux to,be
R2678 T4143 T4136 xcomp be,needs
R2679 T4144 T4143 prep of,be
R2680 T4145 T4146 det a,length
R2681 T4146 T4144 pobj length,of
R2682 T4147 T4146 amod sufficient,length
R2683 T4148 T4143 prep in,be
R2684 T4149 T4148 pobj order,in
R2685 T4150 T4151 aux to,accommodate
R2686 T4151 T4149 acl accommodate,order
R2687 T4152 T4153 det the,complex
R2688 T4153 T4151 dobj complex,accommodate
R2689 T4154 T4153 compound Mcm,complex
R2690 T4155 T4134 punct .,predicts
R2691 T4157 T4158 prep In,showed
R2692 T4159 T4157 pobj fact,In
R2693 T4160 T4158 punct ", ",showed
R2694 T4161 T4158 nsubj we,showed
R2695 T4162 T4158 advmod previously,showed
R2696 T4163 T4164 mark that,forms
R2697 T4164 T4158 ccomp forms,showed
R2698 T4165 T4166 det the,Mcm4
R2699 T4166 T4164 nsubj Mcm4,forms
R2700 T4167 T4166 punct /,Mcm4
R2701 T4168 T4166 nummod 6,Mcm4
R2702 T4169 T4166 punct /,Mcm4
R2703 T4170 T4166 nummod 7,Mcm4
R2704 T4171 T4164 advmod mainly,forms
R2705 T4172 T4173 det a,complex
R2706 T4173 T4164 dobj complex,forms
R2707 T4174 T4175 amod single,hexameric
R2708 T4175 T4173 amod hexameric,complex
R2709 T4176 T4173 prep on,complex
R2710 T4177 T4176 pobj bubble,on
R2711 T4178 T4177 punct -,bubble
R2712 T4179 T4177 nummod 20,bubble
R2713 T4180 T4177 punct (,bubble
R2714 T4181 T4182 det a,substrate
R2715 T4182 T4177 appos substrate,bubble
R2716 T4183 T4182 compound bubble,substrate
R2717 T4184 T4182 prep with,substrate
R2718 T4185 T4186 nummod 20,nt
R2719 T4186 T4187 npadvmod nt,long
R2720 T4187 T4188 amod long,segment
R2721 T4188 T4184 pobj segment,with
R2722 T4189 T4188 amod central,segment
R2723 T4190 T4191 amod single,stranded
R2724 T4191 T4188 amod stranded,segment
R2725 T4192 T4191 punct -,stranded
R2726 T4193 T4173 punct ),complex
R2727 T4194 T4173 cc and,complex
R2728 T4195 T4196 det a,complex
R2729 T4196 T4173 conj complex,complex
R2730 T4197 T4198 amod double,hexameric
R2731 T4198 T4196 amod hexameric,complex
R2732 T4199 T4196 prep on,complex
R2733 T4200 T4201 nmod bubble,DNA
R2734 T4201 T4199 pobj DNA,on
R2735 T4202 T4200 punct -,bubble
R2736 T4203 T4200 nummod 60,bubble
R2737 T4204 T4205 punct (,13
R2738 T4205 T4196 parataxis 13,complex
R2739 T4206 T4205 punct ),13
R2740 T4207 T4158 punct .,showed
R2741 T4209 T4210 prep In,constructed
R2742 T4211 T4209 pobj order,In
R2743 T4212 T4213 aux to,determine
R2744 T4213 T4211 acl determine,order
R2745 T4214 T4215 det the,requirement
R2746 T4215 T4213 dobj requirement,determine
R2747 T4216 T4215 amod minimum,requirement
R2748 T4217 T4215 prep for,requirement
R2749 T4218 T4219 amod double,hexamer
R2750 T4219 T4221 compound hexamer,formation
R2751 T4220 T4219 punct -,hexamer
R2752 T4221 T4217 pobj formation,for
R2753 T4222 T4210 punct ", ",constructed
R2754 T4223 T4224 det a,set
R2755 T4224 T4210 nsubjpass set,constructed
R2756 T4225 T4224 prep of,set
R2757 T4226 T4227 amod synthetic,substrates
R2758 T4227 T4225 pobj substrates,of
R2759 T4228 T4229 npadvmod bubble,like
R2760 T4229 T4227 amod like,substrates
R2761 T4230 T4229 punct -,like
R2762 T4231 T4227 punct (,substrates
R2763 T4232 T4233 compound Bub,T10
R2764 T4233 T4227 appos T10,substrates
R2765 T4234 T4233 punct -,T10
R2766 T4235 T4233 punct ", ",T10
R2767 T4236 T4237 compound Bub,T20
R2768 T4237 T4233 conj T20,T10
R2769 T4238 T4237 punct -,T20
R2770 T4239 T4237 punct ", ",T20
R2771 T4240 T4241 compound Bub,T30
R2772 T4241 T4237 conj T30,T20
R2773 T4242 T4241 punct -,T30
R2774 T4243 T4241 punct ", ",T30
R2775 T4244 T4245 compound Bub,T40
R2776 T4245 T4241 conj T40,T30
R2777 T4246 T4245 punct -,T40
R2778 T4247 T4245 cc and,T40
R2779 T4248 T4249 compound Bub,T50
R278 T757 T758 compound DNA,helicases
R2780 T4249 T4245 conj T50,T40
R2781 T4250 T4249 punct -,T50
R2782 T4251 T4227 punct ),substrates
R2783 T4252 T4253 dep that,differed
R2784 T4253 T4227 relcl differed,substrates
R2785 T4254 T4253 prep in,differed
R2786 T4255 T4256 det the,length
R2787 T4256 T4254 pobj length,in
R2788 T4257 T4256 prep of,length
R2789 T4258 T4259 det the,segment
R279 T758 T759 nsubj helicases,couple
R2790 T4259 T4257 pobj segment,of
R2791 T4260 T4259 amod central,segment
R2792 T4261 T4259 amod unpaired,segment
R2793 T4262 T4210 auxpass were,constructed
R2794 T4263 T4210 punct ", ",constructed
R2795 T4264 T4210 cc and,constructed
R2796 T4265 T4266 det the,activities
R2797 T4266 T4270 nsubjpass activities,examined
R2798 T4267 T4266 nmod binding,activities
R2799 T4268 T4267 cc and,binding
R280 T760 T761 det the,hydrolysis
R2800 T4269 T4267 conj helicase,binding
R2801 T4270 T4210 conj examined,constructed
R2802 T4271 T4266 prep of,activities
R2803 T4272 T4271 pobj Mcm4,of
R2804 T4273 T4272 punct /,Mcm4
R2805 T4274 T4272 nummod 6,Mcm4
R2806 T4275 T4272 punct /,Mcm4
R2807 T4276 T4272 nummod 7,Mcm4
R2808 T4277 T4266 prep on,activities
R2809 T4278 T4279 det these,substrates
R281 T761 T759 dobj hydrolysis,couple
R2810 T4279 T4277 pobj substrates,on
R2811 T4280 T4270 auxpass were,examined
R2812 T4281 T4210 punct .,constructed
R2813 T4283 T4284 nsubjpass Mcm4,incubated
R2814 T4285 T4283 punct /,Mcm4
R2815 T4286 T4283 nummod 6,Mcm4
R2816 T4287 T4283 punct /,Mcm4
R2817 T4288 T4283 nummod 7,Mcm4
R2818 T4289 T4284 auxpass was,incubated
R2819 T4290 T4284 prep with,incubated
R282 T762 T761 prep of,hydrolysis
R2820 T4291 T4292 det a,substrate
R2821 T4292 T4290 pobj substrate,with
R2822 T4293 T4292 amod radiolabeled,substrate
R2823 T4294 T4292 amod synthetic,substrate
R2824 T4295 T4292 compound bubble,substrate
R2825 T4296 T4284 prep in,incubated
R2826 T4297 T4298 det the,presence
R2827 T4298 T4296 pobj presence,in
R2828 T4299 T4298 prep of,presence
R2829 T4300 T4301 nummod 0.5,mM
R283 T763 T764 nmod nucleotide,triphosphate
R2830 T4301 T4302 nmod mM,S
R2831 T4302 T4299 pobj S,of
R2832 T4303 T4302 nmod ATP,S
R2833 T4304 T4302 punct -,S
R2834 T4305 T4302 punct γ,S
R2835 T4306 T4302 punct -,S
R2836 T4307 T4308 aux to,allow
R2837 T4308 T4284 advcl allow,incubated
R2838 T4309 T4310 compound complex,formation
R2839 T4310 T4308 dobj formation,allow
R284 T764 T762 pobj triphosphate,of
R2840 T4311 T4284 punct .,incubated
R2841 T4313 T4314 det A,half
R2842 T4314 T4315 nsubjpass half,analyzed
R2843 T4316 T4314 prep of,half
R2844 T4317 T4318 det these,mixtures
R2845 T4318 T4316 pobj mixtures,of
R2846 T4319 T4318 compound reaction,mixtures
R2847 T4320 T4315 auxpass were,analyzed
R2848 T4321 T4315 prep for,analyzed
R2849 T4322 T4323 compound DNA,binding
R285 T765 T763 cc or,nucleotide
R2850 T4323 T4321 pobj binding,for
R2851 T4324 T4315 prep in,analyzed
R2852 T4325 T4326 compound gel,shift
R2853 T4326 T4327 compound shift,assays
R2854 T4327 T4324 pobj assays,in
R2855 T4328 T4329 punct (,2A
R2856 T4329 T4315 parataxis 2A,analyzed
R2857 T4330 T4329 compound Figure,2A
R2858 T4331 T4329 punct ),2A
R2859 T4332 T4315 punct ", ",analyzed
R286 T766 T763 conj deoxynucleotide,nucleotide
R2860 T4333 T4315 cc and,analyzed
R2861 T4334 T4335 det the,remainder
R2862 T4335 T4336 nsubjpass remainder,incubated
R2863 T4336 T4315 conj incubated,analyzed
R2864 T4337 T4336 auxpass was,incubated
R2865 T4338 T4336 advmod further,incubated
R2866 T4339 T4336 prep in,incubated
R2867 T4340 T4341 det the,presence
R2868 T4341 T4339 pobj presence,in
R2869 T4342 T4341 prep of,presence
R287 T767 T759 prep to,couple
R2870 T4343 T4344 nummod 10,mM
R2871 T4344 T4345 compound mM,ATP
R2872 T4345 T4342 pobj ATP,of
R2873 T4346 T4347 aux to,measure
R2874 T4347 T4336 advcl measure,incubated
R2875 T4348 T4349 compound DNA,helicase
R2876 T4349 T4350 compound helicase,activity
R2877 T4350 T4347 dobj activity,measure
R2878 T4351 T4352 punct (,2B
R2879 T4352 T4347 parataxis 2B,measure
R288 T768 T769 compound duplex,unwinding
R2880 T4353 T4352 compound Figure,2B
R2881 T4354 T4352 punct ),2B
R2882 T4355 T4336 punct .,incubated
R2883 T4357 T4358 nsubj Bub,generated
R2884 T4359 T4357 punct -,Bub
R2885 T4360 T4357 nummod 10,Bub
R2886 T4361 T4362 advmod only,level
R2887 T4362 T4358 dobj level,generated
R2888 T4363 T4362 det a,level
R2889 T4364 T4362 amod low,level
R289 T769 T767 pobj unwinding,to
R2890 T4365 T4362 prep of,level
R2891 T4366 T4365 pobj complexes,of
R2892 T4367 T4366 prep with,complexes
R2893 T4368 T4367 pobj Mcm4,with
R2894 T4369 T4368 punct /,Mcm4
R2895 T4370 T4368 nummod 6,Mcm4
R2896 T4371 T4368 punct /,Mcm4
R2897 T4372 T4368 nummod 7,Mcm4
R2898 T4373 T4358 punct ", ",generated
R2899 T4374 T4358 cc and,generated
R290 T770 T771 punct (,1
R2900 T4375 T4376 nsubj Bub,generated
R2901 T4376 T4358 conj generated,generated
R2902 T4377 T4375 punct -,Bub
R2903 T4378 T4375 nummod 20,Bub
R2904 T4379 T4376 advmod primarily,generated
R2905 T4380 T4381 det a,form
R2906 T4381 T4376 dobj form,generated
R2907 T4382 T4381 amod single,form
R2908 T4383 T4384 npadvmod mobility,shifted
R2909 T4384 T4381 amod shifted,form
R291 T771 T759 parataxis 1,couple
R2910 T4385 T4384 punct -,shifted
R2911 T4386 T4381 punct ", ",form
R2912 T4387 T4388 dep which,contain
R2913 T4388 T4381 relcl contain,form
R2914 T4389 T4388 aux may,contain
R2915 T4390 T4391 det a,hexamer
R2916 T4391 T4388 dobj hexamer,contain
R2917 T4392 T4391 amod single,hexamer
R2918 T4393 T4391 prep of,hexamer
R2919 T4394 T4393 pobj Mcm4,of
R292 T772 T771 punct ),1
R2920 T4395 T4394 punct /,Mcm4
R2921 T4396 T4394 nummod 6,Mcm4
R2922 T4397 T4394 punct /,Mcm4
R2923 T4398 T4394 nummod 7,Mcm4
R2924 T4399 T4376 punct .,generated
R2925 T4401 T4402 prep On,generated
R2926 T4403 T4401 pobj Bub,On
R2927 T4404 T4403 punct -,Bub
R2928 T4405 T4403 nummod 30,Bub
R2929 T4406 T4402 punct ", ",generated
R293 T773 T759 punct .,couple
R2930 T4407 T4408 amod significant,amount
R2931 T4408 T4402 nsubjpass amount,generated
R2932 T4409 T4408 prep of,amount
R2933 T4410 T4409 pobj complexes,of
R2934 T4411 T4410 acl containing,complexes
R2935 T4412 T4413 det a,hexamer
R2936 T4413 T4411 dobj hexamer,containing
R2937 T4414 T4413 amod double,hexamer
R2938 T4415 T4402 auxpass were,generated
R2939 T4416 T4402 prep in,generated
R294 T775 T776 nsubjpass Coordination,required
R2940 T4417 T4416 pobj addition,in
R2941 T4418 T4417 prep to,addition
R2942 T4419 T4418 pobj those,to
R2943 T4420 T4419 acl containing,those
R2944 T4421 T4422 det a,hexamer
R2945 T4422 T4420 dobj hexamer,containing
R2946 T4423 T4422 amod single,hexamer
R2947 T4424 T4425 punct (,2A
R2948 T4425 T4402 parataxis 2A,generated
R2949 T4426 T4425 compound Figure,2A
R295 T777 T775 prep of,Coordination
R2950 T4427 T4425 punct ),2A
R2951 T4428 T4429 punct (,21
R2952 T4429 T4402 parataxis 21,generated
R2953 T4430 T4429 nummod 13,21
R2954 T4431 T4429 punct ",",21
R2955 T4432 T4429 punct ),21
R2956 T4433 T4402 punct .,generated
R2957 T4435 T4436 det The,amounts
R2958 T4436 T4437 nsubj amounts,increased
R2959 T4438 T4436 prep of,amounts
R296 T778 T779 det a,set
R2960 T4439 T4440 amod double,hexamer
R2961 T4440 T4441 compound hexamer,complexes
R2962 T4441 T4438 pobj complexes,of
R2963 T4442 T4437 prep on,increased
R2964 T4443 T4444 compound Bub,T40
R2965 T4444 T4442 pobj T40,on
R2966 T4445 T4444 punct -,T40
R2967 T4446 T4444 cc and,T40
R2968 T4447 T4448 compound Bub,T50
R2969 T4448 T4444 conj T50,T40
R297 T779 T777 pobj set,of
R2970 T4449 T4448 punct -,T50
R2971 T4450 T4437 prep with,increased
R2972 T4451 T4452 amod concomitant,decrease
R2973 T4452 T4450 pobj decrease,with
R2974 T4453 T4452 prep of,decrease
R2975 T4454 T4455 amod single,hexamer
R2976 T4455 T4456 compound hexamer,complexes
R2977 T4456 T4453 pobj complexes,of
R2978 T4457 T4458 punct (,2A
R2979 T4458 T4437 parataxis 2A,increased
R298 T780 T779 prep of,set
R2980 T4459 T4458 compound Figure,2A
R2981 T4460 T4458 punct ),2A
R2982 T4461 T4437 punct .,increased
R2983 T4463 T4464 det The,results
R2984 T4464 T4465 nsubj results,indicate
R2985 T4466 T4467 mark that,required
R2986 T4467 T4465 ccomp required,indicate
R2987 T4468 T4469 det a,segment
R2988 T4469 T4467 nsubjpass segment,required
R2989 T4470 T4471 amod single,stranded
R299 T781 T780 pobj subactivities,of
R2990 T4471 T4469 amod stranded,segment
R2991 T4472 T4471 punct -,stranded
R2992 T4473 T4469 prep of,segment
R2993 T4474 T4475 advmod at,least
R2994 T4475 T4476 advmod least,40
R2995 T4476 T4477 nummod 40,nt
R2996 T4477 T4473 pobj nt,of
R2997 T4478 T4477 amod long,nt
R2998 T4479 T4467 auxpass is,required
R2999 T4480 T4467 prep for,required
R300 T782 T781 prep including,subactivities
R3000 T4481 T4482 amod efficient,loading
R3001 T4482 T4480 pobj loading,for
R3002 T4483 T4482 prep of,loading
R3003 T4484 T4485 det the,helicase
R3004 T4485 T4483 pobj helicase,of
R3005 T4486 T4487 amod double,hexameric
R3006 T4487 T4485 amod hexameric,helicase
R3007 T4488 T4487 punct -,hexameric
R3008 T4489 T4485 compound Mcm,helicase
R3009 T4490 T4482 prep on,loading
R301 T783 T784 compound nucleotide,binding
R3010 T4491 T4492 det a,substrate
R3011 T4492 T4490 pobj substrate,on
R3012 T4493 T4492 compound bubble,substrate
R3013 T4494 T4465 punct .,indicate
R3014 T4496 T4497 prep In,show
R3015 T4498 T4499 compound DNA,helicase
R3016 T4499 T4500 compound helicase,assays
R3017 T4500 T4496 pobj assays,In
R3018 T4501 T4497 punct ", ",show
R3019 T4502 T4503 nmod Mcm4,complex
R302 T784 T782 pobj binding,including
R3020 T4503 T4497 nsubj complex,show
R3021 T4504 T4502 punct /,Mcm4
R3022 T4505 T4502 nummod 6,Mcm4
R3023 T4506 T4502 punct /,Mcm4
R3024 T4507 T4502 nummod 7,Mcm4
R3025 T4508 T4497 aux did,show
R3026 T4509 T4497 neg not,show
R3027 T4510 T4511 compound helicase,activity
R3028 T4511 T4497 dobj activity,show
R3029 T4512 T4497 prep on,show
R303 T785 T784 punct ", ",binding
R3030 T4513 T4514 preconj either,Bub
R3031 T4514 T4515 nmod Bub,substrate
R3032 T4515 T4512 pobj substrate,on
R3033 T4516 T4514 punct -,Bub
R3034 T4517 T4514 nummod 10,Bub
R3035 T4518 T4514 cc or,Bub
R3036 T4519 T4514 conj Bub,Bub
R3037 T4520 T4519 punct -,Bub
R3038 T4521 T4519 nummod 20,Bub
R3039 T4522 T4497 punct .,show
R304 T786 T787 compound DNA,binding
R3040 T4524 T4525 det The,efficiency
R3041 T4525 T4526 nsubj efficiency,improved
R3042 T4527 T4525 prep of,efficiency
R3043 T4528 T4527 pobj displacement,of
R3044 T4529 T4526 advmod slightly,improved
R3045 T4530 T4531 mark as,increased
R3046 T4531 T4526 advcl increased,improved
R3047 T4532 T4533 det the,length
R3048 T4533 T4531 nsubj length,increased
R3049 T4534 T4533 prep of,length
R305 T787 T784 conj binding,binding
R3050 T4535 T4536 det the,segment
R3051 T4536 T4534 pobj segment,of
R3052 T4537 T4538 amod single,stranded
R3053 T4538 T4536 amod stranded,segment
R3054 T4539 T4538 punct -,stranded
R3055 T4540 T4541 quantmod up,30
R3056 T4541 T4543 nummod 30,nt
R3057 T4542 T4541 quantmod to,30
R3058 T4543 T4531 npadvmod nt,increased
R3059 T4544 T4526 punct .,improved
R306 T788 T787 cc and,binding
R3060 T4546 T4547 prep On,observed
R3061 T4548 T4549 compound Bub,T40
R3062 T4549 T4546 pobj T40,On
R3063 T4550 T4549 punct -,T40
R3064 T4551 T4549 cc and,T40
R3065 T4552 T4553 compound Bub,T50
R3066 T4553 T4549 conj T50,T40
R3067 T4554 T4553 punct -,T50
R3068 T4555 T4547 punct ", ",observed
R3069 T4556 T4547 prep on,observed
R307 T789 T790 compound ATP,hydrolysis
R3070 T4557 T4558 det the,hand
R3071 T4558 T4556 pobj hand,on
R3072 T4559 T4558 amod other,hand
R3073 T4560 T4547 punct ", ",observed
R3074 T4561 T4562 amod significant,displacement
R3075 T4562 T4547 nsubjpass displacement,observed
R3076 T4563 T4547 auxpass was,observed
R3077 T4564 T4547 punct ", ",observed
R3078 T4565 T4547 advcl indicating,observed
R3079 T4566 T4567 mark that,required
R308 T790 T787 conj hydrolysis,binding
R3080 T4567 T4565 ccomp required,indicating
R3081 T4568 T4569 det a,segment
R3082 T4569 T4567 nsubjpass segment,required
R3083 T4570 T4571 amod single,stranded
R3084 T4571 T4569 amod stranded,segment
R3085 T4572 T4571 punct -,stranded
R3086 T4573 T4569 prep of,segment
R3087 T4574 T4575 advmod at,least
R3088 T4575 T4576 advmod least,40
R3089 T4576 T4577 nummod 40,nt
R309 T791 T776 auxpass is,required
R3090 T4577 T4573 pobj nt,of
R3091 T4578 T4577 amod long,nt
R3092 T4579 T4567 auxpass is,required
R3093 T4580 T4567 prep for,required
R3094 T4581 T4582 amod efficient,actions
R3095 T4582 T4580 pobj actions,for
R3096 T4583 T4582 compound helicase,actions
R3097 T4584 T4582 prep on,actions
R3098 T4585 T4586 compound bubble,substrates
R3099 T4586 T4584 pobj substrates,on
R310 T792 T776 prep for,required
R3100 T4587 T4588 punct (,2B
R3101 T4588 T4567 parataxis 2B,required
R3102 T4589 T4588 compound Figure,2B
R3103 T4590 T4588 punct ),2B
R3104 T4591 T4547 punct .,observed
R3105 T4593 T4594 advcl Taken,are
R3106 T4595 T4593 advmod together,Taken
R3107 T4596 T4594 punct ", ",are
R3108 T4597 T4598 det the,results
R3109 T4598 T4594 nsubj results,are
R311 T793 T792 pobj unwinding,for
R3110 T4599 T4598 amod above,results
R3111 T4600 T4594 acomp consistent,are
R3112 T4601 T4600 prep with,consistent
R3113 T4602 T4603 det the,notion
R3114 T4603 T4601 pobj notion,with
R3115 T4604 T4605 mark that,unwinds
R3116 T4605 T4603 acl unwinds,notion
R3117 T4606 T4607 det the,complex
R3118 T4607 T4605 nsubj complex,unwinds
R3119 T4608 T4607 nmod Mcm4,complex
R312 T794 T793 prep of,unwinding
R3120 T4609 T4608 punct /,Mcm4
R3121 T4610 T4608 nummod 6,Mcm4
R3122 T4611 T4608 punct /,Mcm4
R3123 T4612 T4608 nummod 7,Mcm4
R3124 T4613 T4607 punct ", ",complex
R3125 T4614 T4607 acl loaded,complex
R3126 T4615 T4614 prep onto,loaded
R3127 T4616 T4615 pobj DNA,onto
R3128 T4617 T4614 prep as,loaded
R3129 T4618 T4619 det a,complex
R313 T795 T796 compound duplex,DNA
R3130 T4619 T4617 pobj complex,as
R3131 T4620 T4621 amod double,hexameric
R3132 T4621 T4619 amod hexameric,complex
R3133 T4622 T4605 punct ", ",unwinds
R3134 T4623 T4605 advmod efficiently,unwinds
R3135 T4624 T4625 det the,region
R3136 T4625 T4605 dobj region,unwinds
R3137 T4626 T4625 compound duplex,region
R3138 T4627 T4625 prep of,region
R3139 T4628 T4629 det the,DNA
R314 T796 T794 pobj DNA,of
R3140 T4629 T4627 pobj DNA,of
R3141 T4630 T4629 compound substrate,DNA
R3142 T4631 T4594 punct .,are
R3145 T4737 T4738 det Both,strands
R3146 T4738 T4739 nsubjpass strands,required
R3147 T4740 T4738 prep of,strands
R3148 T4741 T4742 npadvmod thymine,rich
R3149 T4742 T4744 amod rich,segments
R315 T797 T798 punct (,2
R3150 T4743 T4742 punct -,rich
R3151 T4744 T4740 pobj segments,of
R3152 T4745 T4746 amod single,stranded
R3153 T4746 T4744 amod stranded,segments
R3154 T4747 T4746 punct -,stranded
R3155 T4748 T4739 auxpass are,required
R3156 T4749 T4739 prep for,required
R3157 T4750 T4751 amod efficient,unwinding
R3158 T4751 T4749 pobj unwinding,for
R3159 T4752 T4751 prep of,unwinding
R316 T798 T776 parataxis 2,required
R3160 T4753 T4754 compound bubble,DNA
R3161 T4754 T4752 pobj DNA,of
R3162 T4755 T4751 prep by,unwinding
R3163 T4756 T4755 pobj Mcm4,by
R3164 T4757 T4756 punct /,Mcm4
R3165 T4758 T4756 nummod 6,Mcm4
R3166 T4759 T4756 punct /,Mcm4
R3167 T4760 T4756 nummod 7,Mcm4
R3168 T4762 T4763 amod Symmetric,binding
R3169 T4763 T4764 nsubj binding,suggests
R317 T799 T798 nummod 1,2
R3170 T4765 T4763 prep of,binding
R3171 T4766 T4767 det the,hexamer
R3172 T4767 T4765 pobj hexamer,of
R3173 T4768 T4767 nmod Mcm4,hexamer
R3174 T4769 T4768 punct /,Mcm4
R3175 T4770 T4768 nummod 6,Mcm4
R3176 T4771 T4768 punct /,Mcm4
R3177 T4772 T4768 nummod 7,Mcm4
R3178 T4773 T4767 amod double,hexamer
R3179 T4774 T4763 prep to,binding
R318 T800 T798 punct ",",2
R3180 T4775 T4774 pobj bubble,to
R3181 T4776 T4777 mark that,catalyzes
R3182 T4777 T4764 ccomp catalyzes,suggests
R3183 T4778 T4779 det each,hexamer
R3184 T4779 T4777 nsubj hexamer,catalyzes
R3185 T4780 T4777 dobj unwinding,catalyzes
R3186 T4781 T4780 prep of,unwinding
R3187 T4782 T4781 pobj one,of
R3188 T4783 T4782 prep of,one
R3189 T4784 T4785 det the,segments
R319 T801 T798 punct ),2
R3190 T4785 T4783 pobj segments,of
R3191 T4786 T4785 nummod two,segments
R3192 T4787 T4785 compound duplex,segments
R3193 T4788 T4764 punct .,suggests
R3194 T4790 T4791 nsubj We,asked
R3195 T4792 T4793 mark whether,required
R3196 T4793 T4791 ccomp required,asked
R3197 T4794 T4793 nsubjpass activation,required
R3198 T4795 T4794 prep of,activation
R3199 T4796 T4797 det both,hexamers
R320 T802 T776 punct .,required
R3200 T4797 T4795 pobj hexamers,of
R3201 T4798 T4793 auxpass is,required
R3202 T4799 T4793 prep for,required
R3203 T4800 T4799 pobj unwinding,for
R3204 T4801 T4800 prep of,unwinding
R3205 T4802 T4803 det the,substrate
R3206 T4803 T4801 pobj substrate,of
R3207 T4804 T4803 compound bubble,substrate
R3208 T4805 T4791 punct .,asked
R3209 T4807 T4808 det The,substrate
R321 T804 T805 det The,helicases
R3210 T4808 T4811 nsubj substrate,contains
R3211 T4809 T4808 amod preferred,substrate
R3212 T4810 T4808 compound bubble,substrate
R3213 T4812 T4813 dep which,unwound
R3214 T4813 T4808 relcl unwound,substrate
R3215 T4814 T4813 auxpass is,unwound
R3216 T4815 T4813 advmod efficiently,unwound
R3217 T4816 T4813 agent by,unwound
R3218 T4817 T4816 pobj Mcm4,by
R3219 T4818 T4817 punct /,Mcm4
R322 T805 T807 nsubjpass helicases,known
R3220 T4819 T4817 nummod 6,Mcm4
R3221 T4820 T4817 punct /,Mcm4
R3222 T4821 T4817 nummod 7,Mcm4
R3223 T4822 T4823 compound thymine,stretches
R3224 T4823 T4811 dobj stretches,contains
R3225 T4824 T4823 prep on,stretches
R3226 T4825 T4826 det both,strands
R3227 T4826 T4824 pobj strands,on
R3228 T4827 T4826 prep of,strands
R3229 T4828 T4829 det the,segment
R323 T806 T805 amod hexameric,helicases
R3230 T4829 T4827 pobj segment,of
R3231 T4830 T4831 amod single,stranded
R3232 T4831 T4829 amod stranded,segment
R3233 T4832 T4831 punct -,stranded
R3234 T4833 T4811 punct .,contains
R3235 T4835 T4836 nsubj We,constructed
R3236 T4837 T4838 det a,hetero-bubble
R3237 T4838 T4836 dobj hetero-bubble,constructed
R3238 T4839 T4838 punct (,hetero-bubble
R3239 T4840 T4841 compound Bub66,G
R324 T808 T805 punct ", ",helicases
R3240 T4841 T4838 appos G,hetero-bubble
R3241 T4842 T4841 punct /,G
R3242 T4843 T4841 compound T,G
R3243 T4844 T4841 punct –,G
R3244 T4845 T4841 punct -,G
R3245 T4846 T4841 amod rich,G
R3246 T4847 T4838 punct ),hetero-bubble
R3247 T4848 T4849 prep in,is
R3248 T4849 T4838 relcl is,hetero-bubble
R3249 T4850 T4848 pobj which,in
R325 T809 T810 prep to,belongs
R3250 T4851 T4852 nummod one,strand
R3251 T4852 T4849 nsubj strand,is
R3252 T4853 T4854 npadvmod T,rich
R3253 T4854 T4849 acomp rich,is
R3254 T4855 T4854 punct -,rich
R3255 T4856 T4857 punct (,same
R3256 T4857 T4854 parataxis same,rich
R3257 T4858 T4857 prep as,same
R3258 T4859 T4860 compound Bub66,T
R3259 T4860 T4858 pobj T,as
R326 T810 T805 relcl belongs,helicases
R3260 T4861 T4860 punct /,T
R3261 T4862 T4860 punct -,T
R3262 T4863 T4860 amod rich,T
R3263 T4864 T4857 punct ),same
R3264 T4865 T4849 cc and,is
R3265 T4866 T4867 det the,strand
R3266 T4867 T4869 nsubj strand,is
R3267 T4868 T4867 amod other,strand
R3268 T4869 T4849 conj is,is
R3269 T4870 T4871 npadvmod G,rich
R327 T811 T809 pobj which,to
R3270 T4871 T4869 acomp rich,is
R3271 T4872 T4871 punct -,rich
R3272 T4873 T4874 punct (,same
R3273 T4874 T4871 parataxis same,rich
R3274 T4875 T4874 prep as,same
R3275 T4876 T4877 compound Bub66,G
R3276 T4877 T4875 pobj G,as
R3277 T4878 T4877 punct /,G
R3278 T4879 T4877 punct -,G
R3279 T4880 T4877 amod rich,G
R328 T812 T813 nmod minichromosome,maintenance
R3280 T4881 T4874 punct ),same
R3281 T4882 T4883 punct (,13
R3282 T4883 T4871 parataxis 13,rich
R3283 T4884 T4883 punct ),13
R3284 T4885 T4836 punct ", ",constructed
R3285 T4886 T4836 cc and,constructed
R3286 T4887 T4888 compound helicase,assays
R3287 T4888 T4889 nsubjpass assays,conducted
R3288 T4889 T4836 conj conducted,constructed
R3289 T4890 T4889 auxpass were,conducted
R329 T813 T814 nmod maintenance,helicase
R3290 T4891 T4889 prep with,conducted
R3291 T4892 T4893 amod increasing,amount
R3292 T4893 T4891 pobj amount,with
R3293 T4894 T4893 prep of,amount
R3294 T4895 T4894 pobj Mcm4,of
R3295 T4896 T4895 punct /,Mcm4
R3296 T4897 T4895 nummod 6,Mcm4
R3297 T4898 T4895 punct /,Mcm4
R3298 T4899 T4895 nummod 7,Mcm4
R3299 T4900 T4889 punct .,conducted
R330 T814 T810 nsubj helicase,belongs
R3300 T4902 T4903 advmod Only,unwinding
R3301 T4903 T4905 nsubjpass unwinding,detected
R3302 T4904 T4903 amod weak,unwinding
R3303 T4906 T4905 auxpass was,detected
R3304 T4907 T4905 prep on,detected
R3305 T4908 T4909 det this,substrate
R3306 T4909 T4907 pobj substrate,on
R3307 T4910 T4905 prep compared,detected
R3308 T4911 T4910 prep to,compared
R3309 T4912 T4913 compound Bub66,T
R331 T815 T813 punct (,maintenance
R3310 T4913 T4911 pobj T,to
R3311 T4914 T4913 punct /,T
R3312 T4915 T4913 punct -,T
R3313 T4916 T4913 amod rich,T
R3314 T4917 T4918 punct (,3A
R3315 T4918 T4905 parataxis 3A,detected
R3316 T4919 T4918 compound Figure,3A
R3317 T4920 T4918 punct ),3A
R3318 T4921 T4905 punct .,detected
R3319 T4923 T4924 mark Since,bind
R332 T816 T813 appos Mcm,maintenance
R3320 T4924 T4931 advcl bind,is
R3321 T4925 T4924 nsubj Mcm4,bind
R3322 T4926 T4925 punct /,Mcm4
R3323 T4927 T4925 nummod 6,Mcm4
R3324 T4928 T4925 punct /,Mcm4
R3325 T4929 T4925 nummod 7,Mcm4
R3326 T4930 T4924 aux can,bind
R3327 T4932 T4924 prep to,bind
R3328 T4933 T4934 compound Bub66,G
R3329 T4934 T4932 pobj G,to
R333 T817 T813 punct ),maintenance
R3330 T4935 T4934 punct /,G
R3331 T4936 T4934 compound T,G
R3332 T4937 T4934 punct –,G
R3333 T4938 T4934 punct -,G
R3334 T4939 T4934 amod rich,G
R3335 T4940 T4924 cc and,bind
R3336 T4941 T4924 conj forms,bind
R3337 T4942 T4943 det a,complex
R3338 T4943 T4941 dobj complex,forms
R3339 T4944 T4945 amod double,hexameric
R334 T818 T807 punct ", ",known
R3340 T4945 T4943 amod hexameric,complex
R3341 T4946 T4947 punct (,shown
R3342 T4947 T4941 parataxis shown,forms
R3343 T4948 T4947 nsubj data,shown
R3344 T4949 T4947 neg not,shown
R3345 T4950 T4947 punct ),shown
R3346 T4951 T4931 punct ", ",is
R3347 T4952 T4953 det the,unwinding
R3348 T4953 T4931 nsubj unwinding,is
R3349 T4954 T4953 amod defective,unwinding
R335 T819 T807 auxpass are,known
R3350 T4955 T4931 neg not,is
R3351 T4956 T4931 prep due,is
R3352 T4957 T4956 pcomp to,due
R3353 T4958 T4959 amod inefficient,loading
R3354 T4959 T4956 pobj loading,due
R3355 T4960 T4959 prep of,loading
R3356 T4961 T4962 det the,helicase
R3357 T4962 T4960 pobj helicase,of
R3358 T4963 T4959 prep onto,loading
R3359 T4964 T4965 det the,substrate
R336 T820 T821 aux to,assemble
R3360 T4965 T4963 pobj substrate,onto
R3361 T4966 T4931 punct .,is
R3362 T4968 T4969 mark If,dissociates
R3363 T4969 T4979 advcl dissociates,result
R3364 T4970 T4971 nummod one,hexamer
R3365 T4971 T4969 nsubj hexamer,dissociates
R3366 T4972 T4971 acl interacting,hexamer
R3367 T4973 T4972 prep with,interacting
R3368 T4974 T4975 det the,strand
R3369 T4975 T4973 pobj strand,with
R337 T821 T807 xcomp assemble,known
R3370 T4976 T4977 npadvmod thymine,rich
R3371 T4977 T4975 amod rich,strand
R3372 T4978 T4977 punct -,rich
R3373 T4980 T4969 prep from,dissociates
R3374 T4981 T4982 det the,hexamer
R3375 T4982 T4980 pobj hexamer,from
R3376 T4983 T4982 amod other,hexamer
R3377 T4984 T4969 cc and,dissociates
R3378 T4985 T4969 conj displaces,dissociates
R3379 T4986 T4987 advmod only,one
R338 T822 T823 det a,structure
R3380 T4987 T4985 dobj one,displaces
R3381 T4988 T4987 prep of,one
R3382 T4989 T4990 det the,segments
R3383 T4990 T4988 pobj segments,of
R3384 T4991 T4990 compound duplex,segments
R3385 T4992 T4979 punct ", ",result
R3386 T4993 T4979 advmod then,result
R3387 T4994 T4979 nsubj it,result
R3388 T4995 T4979 aux would,result
R3389 T4996 T4979 prep in,result
R339 T823 T821 dobj structure,assemble
R3390 T4997 T4996 pobj generation,in
R3391 T4998 T4997 prep of,generation
R3392 T4999 T5000 compound Y,fork
R3393 T5000 T5002 compound fork,structures
R3394 T5001 T5000 punct -,fork
R3395 T5002 T4998 pobj structures,of
R3396 T5003 T5004 dep which,displaced
R3397 T5004 T5002 relcl displaced,structures
R3398 T5005 T5004 aux could,displaced
R3399 T5006 T5004 neg not,displaced
R340 T824 T825 npadvmod ring,like
R3400 T5007 T5004 auxpass be,displaced
R3401 T5008 T5004 advmod further,displaced
R3402 T5009 T5004 prep due,displaced
R3403 T5010 T5009 pcomp to,due
R3404 T5011 T5012 det the,presence
R3405 T5012 T5009 pobj presence,due
R3406 T5013 T5012 prep of,presence
R3407 T5014 T5015 npadvmod G,rich
R3408 T5015 T5017 amod rich,tail
R3409 T5016 T5015 punct -,rich
R341 T825 T823 amod like,structure
R3410 T5017 T5013 pobj tail,of
R3411 T5018 T5017 nummod 3,tail
R3412 T5019 T5018 punct ′,3
R3413 T5020 T5017 punct -,tail
R3414 T5021 T5004 cc and,displaced
R3415 T5022 T5023 aux would,appear
R3416 T5023 T5004 conj appear,displaced
R3417 T5024 T5023 prep on,appear
R3418 T5025 T5026 det the,gel
R3419 T5026 T5024 pobj gel,on
R342 T826 T825 punct -,like
R3420 T5027 T4979 punct .,result
R3421 T5029 T5030 advmod However,detect
R3422 T5031 T5030 punct ", ",detect
R3423 T5032 T5030 nsubj we,detect
R3424 T5033 T5030 aux did,detect
R3425 T5034 T5030 neg not,detect
R3426 T5035 T5036 amod such,intermediates
R3427 T5036 T5030 dobj intermediates,detect
R3428 T5037 T5030 prep during,detect
R3429 T5038 T5039 det the,course
R343 T827 T828 punct (,3
R3430 T5039 T5037 pobj course,during
R3431 T5040 T5039 prep of,course
R3432 T5041 T5042 det the,experiment
R3433 T5042 T5040 pobj experiment,of
R3434 T5043 T5044 punct (,3B
R3435 T5044 T5030 parataxis 3B,detect
R3436 T5045 T5044 compound Figure,3B
R3437 T5046 T5044 punct ),3B
R3438 T5047 T5030 punct ", ",detect
R3439 T5048 T5030 advcl suggesting,detect
R344 T828 T807 parataxis 3,known
R3440 T5049 T5050 mark that,displaced
R3441 T5050 T5048 ccomp displaced,suggesting
R3442 T5051 T5050 nsubjpass bubble,displaced
R3443 T5052 T5050 aux can,displaced
R3444 T5053 T5050 auxpass be,displaced
R3445 T5054 T5050 advmod efficiently,displaced
R3446 T5055 T5056 advmod only,activated
R3447 T5056 T5050 advcl activated,displaced
R3448 T5057 T5056 advmod when,activated
R3449 T5058 T5059 nummod two,molecules
R345 T829 T830 punct –,5
R3450 T5059 T5056 nsubjpass molecules,activated
R3451 T5060 T5059 prep of,molecules
R3452 T5061 T5062 det the,hexamers
R3453 T5062 T5060 pobj hexamers,of
R3454 T5063 T5062 nmod Mcm4,hexamers
R3455 T5064 T5063 punct /,Mcm4
R3456 T5065 T5063 nummod 6,Mcm4
R3457 T5066 T5063 punct /,Mcm4
R3458 T5067 T5063 nummod 7,Mcm4
R3459 T5068 T5056 auxpass are,activated
R346 T830 T828 prep 5,3
R3460 T5069 T5056 advmod simultaneously,activated
R3461 T5070 T5030 punct .,detect
R3464 T5260 T5261 nmod Binding,actions
R3465 T5262 T5260 cc and,Binding
R3466 T5263 T5260 conj helicase,Binding
R3467 T5264 T5261 prep of,actions
R3468 T5265 T5264 pobj Mcm4,of
R3469 T5266 T5265 punct /,Mcm4
R347 T831 T828 punct ),3
R3470 T5267 T5265 nummod 6,Mcm4
R3471 T5268 T5265 punct /,Mcm4
R3472 T5269 T5265 nummod 7,Mcm4
R3473 T5270 T5261 prep on,actions
R3474 T5271 T5272 amod forked,substrates
R3475 T5272 T5270 pobj substrates,on
R3476 T5273 T5271 cc and,forked
R3477 T5274 T5275 nummod 3,extension
R3478 T5275 T5271 conj extension,forked
R3479 T5276 T5274 punct ′,3
R348 T832 T807 punct .,known
R3480 T5277 T5274 punct -,3
R3481 T5278 T5274 cc or,3
R3482 T5279 T5274 conj 5,3
R3483 T5280 T5279 punct ′,5
R3484 T5281 T5275 punct -,extension
R3485 T5283 T5284 prep In,constructed
R3486 T5285 T5283 pobj order,In
R3487 T5286 T5287 aux to,examine
R3488 T5287 T5285 acl examine,order
R3489 T5288 T5289 advmod more,precisely
R349 T834 T835 mark Whereas,composed
R3490 T5289 T5287 advmod precisely,examine
R3491 T5290 T5291 det the,interaction
R3492 T5291 T5287 dobj interaction,examine
R3493 T5292 T5291 cc and,interaction
R3494 T5293 T5294 compound helicase,actions
R3495 T5294 T5291 conj actions,interaction
R3496 T5295 T5291 prep with,interaction
R3497 T5296 T5297 amod forked,substrates
R3498 T5297 T5295 pobj substrates,with
R3499 T5298 T5284 punct ", ",constructed
R350 T835 T874 advcl composed,composed
R3500 T5299 T5300 compound Y,fork
R3501 T5300 T5302 compound fork,substrates
R3502 T5301 T5300 punct -,fork
R3503 T5302 T5284 nsubjpass substrates,constructed
R3504 T5303 T5302 acl containing,substrates
R3505 T5304 T5305 det a,strand
R3506 T5305 T5303 dobj strand,containing
R3507 T5306 T5305 amod nascent,strand
R3508 T5307 T5305 amod leading,strand
R3509 T5308 T5305 punct (,strand
R351 T836 T835 nsubjpass most,composed
R3510 T5309 T5310 compound A,fork
R3511 T5310 T5305 appos fork,strand
R3512 T5311 T5310 punct -,fork
R3513 T5312 T5310 punct [,fork
R3514 T5313 T5310 nummod 3,fork
R3515 T5314 T5310 punct ′,fork
R3516 T5315 T5305 punct ],strand
R3517 T5316 T5305 punct ),strand
R3518 T5317 T5305 punct ", ",strand
R3519 T5318 T5319 amod nascent,strand
R352 T837 T836 prep of,most
R3520 T5319 T5305 appos strand,strand
R3521 T5320 T5319 amod lagging,strand
R3522 T5321 T5319 punct (,strand
R3523 T5322 T5323 compound A,fork
R3524 T5323 T5319 appos fork,strand
R3525 T5324 T5323 punct -,fork
R3526 T5325 T5323 punct [,fork
R3527 T5326 T5323 nummod 5,fork
R3528 T5327 T5323 punct ′,fork
R3529 T5328 T5305 punct ],strand
R353 T838 T839 compound DNA,helicases
R3530 T5329 T5305 punct ),strand
R3531 T5330 T5305 punct ", ",strand
R3532 T5331 T5305 cc or,strand
R3533 T5332 T5305 conj both,strand
R3534 T5333 T5332 prep of,both
R3535 T5334 T5333 pobj them,of
R3536 T5335 T5332 punct (,both
R3537 T5336 T5337 nmod A,fork
R3538 T5337 T5339 nmod fork,structures
R3539 T5338 T5337 punct -,fork
R354 T839 T837 pobj helicases,of
R3540 T5339 T5332 appos structures,both
R3541 T5340 T5337 punct [,fork
R3542 T5341 T5337 appos 3,fork
R3543 T5342 T5341 punct ′,3
R3544 T5343 T5341 punct ",",3
R3545 T5344 T5341 appos 5,3
R3546 T5345 T5344 punct ′,5
R3547 T5346 T5337 punct ],fork
R3548 T5347 T5337 punct ),fork
R3549 T5348 T5337 punct ", ",fork
R355 T840 T839 prep of,helicases
R3550 T5349 T5350 advmod as,as
R3551 T5350 T5337 cc as,fork
R3552 T5351 T5350 advmod well,as
R3553 T5352 T5353 nummod 5,extension
R3554 T5353 T5337 conj extension,fork
R3555 T5354 T5352 punct ′,5
R3556 T5355 T5353 punct -,extension
R3557 T5356 T5353 cc or,extension
R3558 T5357 T5358 nummod 3,extension
R3559 T5358 T5353 conj extension,extension
R356 T841 T842 det this,group
R3560 T5359 T5357 punct ′,3
R3561 T5360 T5358 punct -,extension
R3562 T5361 T5284 auxpass were,constructed
R3563 T5362 T5284 punct .,constructed
R3564 T5364 T5365 nsubj Mcm4,bound
R3565 T5366 T5364 punct /,Mcm4
R3566 T5367 T5364 nummod 6,Mcm4
R3567 T5368 T5364 punct /,Mcm4
R3568 T5369 T5364 nummod 7,Mcm4
R3569 T5370 T5365 prep to,bound
R357 T842 T840 pobj group,of
R3570 T5371 T5372 compound A,fork
R3571 T5372 T5370 pobj fork,to
R3572 T5373 T5372 punct -,fork
R3573 T5374 T5372 punct [,fork
R3574 T5375 T5372 nummod 3,fork
R3575 T5376 T5372 punct ′,fork
R3576 T5377 T5372 punct ],fork
R3577 T5378 T5372 cc or,fork
R3578 T5379 T5380 compound A,fork
R3579 T5380 T5372 conj fork,fork
R358 T843 T836 punct ", ",most
R3580 T5381 T5380 punct -,fork
R3581 T5382 T5380 punct [,fork
R3582 T5383 T5380 nummod 5,fork
R3583 T5384 T5380 punct ′,fork
R3584 T5385 T5365 punct ],bound
R3585 T5386 T5365 prep with,bound
R3586 T5387 T5386 pobj affinity,with
R3587 T5388 T5387 amod similar,affinity
R3588 T5389 T5388 prep to,similar
R3589 T5390 T5389 pobj that,to
R359 T844 T836 prep including,most
R3590 T5391 T5390 prep of,that
R3591 T5392 T5393 det a,fork
R3592 T5393 T5391 pobj fork,of
R3593 T5394 T5393 amod simple,fork
R3594 T5395 T5393 compound Y,fork
R3595 T5396 T5393 punct -,fork
R3596 T5397 T5398 punct (,4A
R3597 T5398 T5365 parataxis 4A,bound
R3598 T5399 T5398 compound Figure,4A
R3599 T5400 T5398 cc and,4A
R360 T845 T846 amod simian,virus
R3600 T5401 T5402 nsubj data,shown
R3601 T5402 T5398 conj shown,4A
R3602 T5403 T5402 neg not,shown
R3603 T5404 T5398 punct ),4A
R3604 T5405 T5365 punct .,bound
R3605 T5407 T5408 prep In,bound
R3606 T5409 T5407 pobj contrast,In
R3607 T5410 T5408 punct ", ",bound
R3608 T5411 T5412 compound PriA,helicase
R3609 T5412 T5408 nsubj helicase,bound
R361 T846 T847 nmod virus,antigen
R3610 T5413 T5412 punct ", ",helicase
R3611 T5414 T5412 acl used,helicase
R3612 T5415 T5414 prep as,used
R3613 T5416 T5417 det a,control
R3614 T5417 T5415 pobj control,as
R3615 T5418 T5408 punct ", ",bound
R3616 T5419 T5408 prep to,bound
R3617 T5420 T5421 compound A,fork
R3618 T5421 T5419 pobj fork,to
R3619 T5422 T5421 punct -,fork
R362 T847 T844 pobj antigen,including
R3620 T5423 T5421 punct [,fork
R3621 T5424 T5421 nummod 3,fork
R3622 T5425 T5421 punct ′,fork
R3623 T5426 T5408 punct ],bound
R3624 T5427 T5428 advmod more,efficiently
R3625 T5428 T5408 advmod efficiently,bound
R3626 T5429 T5428 prep than,efficiently
R3627 T5430 T5429 prep to,than
R3628 T5431 T5432 compound A,fork
R3629 T5432 T5430 pobj fork,to
R363 T848 T846 nummod 40,virus
R3630 T5433 T5432 punct -,fork
R3631 T5434 T5432 punct [,fork
R3632 T5435 T5432 nummod 5,fork
R3633 T5436 T5432 punct ′,fork
R3634 T5437 T5408 punct ],bound
R3635 T5438 T5408 punct ", ",bound
R3636 T5439 T5440 mark as,reported
R3637 T5440 T5408 advcl reported,bound
R3638 T5441 T5440 advmod previously,reported
R3639 T5442 T5443 punct (,25
R364 T849 T847 amod large,antigen
R3640 T5443 T5440 parataxis 25,reported
R3641 T5444 T5443 nummod 23,25
R3642 T5445 T5443 punct ",",25
R3643 T5446 T5443 punct ),25
R3644 T5447 T5408 punct .,bound
R3645 T5449 T5450 nsubj Mcm4,bound
R3646 T5451 T5449 punct /,Mcm4
R3647 T5452 T5449 nummod 6,Mcm4
R3648 T5453 T5449 punct /,Mcm4
R3649 T5454 T5449 nummod 7,Mcm4
R365 T850 T847 compound T,antigen
R3650 T5455 T5450 advmod also,bound
R3651 T5456 T5450 prep to,bound
R3652 T5457 T5458 nummod 5,extension
R3653 T5458 T5456 pobj extension,to
R3654 T5459 T5457 punct ′,5
R3655 T5460 T5458 punct -,extension
R3656 T5461 T5458 cc and,extension
R3657 T5462 T5463 nummod 3,extension
R3658 T5463 T5458 conj extension,extension
R3659 T5464 T5462 punct ′,3
R366 T851 T847 punct -,antigen
R3660 T5465 T5463 punct -,extension
R3661 T5466 T5450 punct ", ",bound
R3662 T5467 T5468 dep albeit,with
R3663 T5468 T5450 prep with,bound
R3664 T5469 T5470 advmod slightly,reduced
R3665 T5470 T5471 amod reduced,affinity
R3666 T5471 T5468 pobj affinity,with
R3667 T5472 T5471 prep compared,affinity
R3668 T5473 T5472 prep to,compared
R3669 T5474 T5475 compound A,fork
R367 T852 T853 punct (,6
R3670 T5475 T5473 pobj fork,to
R3671 T5476 T5475 punct -,fork
R3672 T5477 T5475 punct [,fork
R3673 T5478 T5475 nummod 3,fork
R3674 T5479 T5475 punct ′,fork
R3675 T5480 T5475 punct ],fork
R3676 T5481 T5475 cc and,fork
R3677 T5482 T5483 compound A,fork
R3678 T5483 T5475 conj fork,fork
R3679 T5484 T5483 punct -,fork
R368 T853 T847 parataxis 6,antigen
R3680 T5485 T5483 punct [,fork
R3681 T5486 T5483 nummod 5,fork
R3682 T5487 T5483 punct ′,fork
R3683 T5488 T5450 punct ],bound
R3684 T5489 T5490 punct (,4A
R3685 T5490 T5450 parataxis 4A,bound
R3686 T5491 T5490 compound Figure,4A
R3687 T5492 T5490 punct ),4A
R3688 T5493 T5450 punct .,bound
R3689 T5495 T5496 nsubj Mcm4,bound
R369 T854 T853 punct ),6
R3690 T5497 T5495 punct /,Mcm4
R3691 T5498 T5495 nummod 6,Mcm4
R3692 T5499 T5495 punct /,Mcm4
R3693 T5500 T5495 nummod 7,Mcm4
R3694 T5501 T5496 prep to,bound
R3695 T5502 T5503 compound A,fork
R3696 T5503 T5501 pobj fork,to
R3697 T5504 T5503 punct -,fork
R3698 T5505 T5503 punct [,fork
R3699 T5506 T5503 appos 3,fork
R370 T855 T847 punct ", ",antigen
R3700 T5507 T5506 punct ′,3
R3701 T5508 T5506 punct ",",3
R3702 T5509 T5506 appos 5,3
R3703 T5510 T5509 punct ′,5
R3704 T5511 T5496 punct ],bound
R3705 T5512 T5496 prep with,bound
R3706 T5513 T5514 advmod much,lower
R3707 T5514 T5515 amod lower,affinity
R3708 T5515 T5512 pobj affinity,with
R3709 T5516 T5496 punct ", ",bound
R371 T856 T857 compound Escherichia,coli
R3710 T5517 T5496 cc and,bound
R3711 T5518 T5519 det the,complex
R3712 T5519 T5520 nsubj complex,gave
R3713 T5520 T5496 conj gave,bound
R3714 T5521 T5520 dobj rise,gave
R3715 T5522 T5520 prep to,gave
R3716 T5523 T5524 det a,band
R3717 T5524 T5522 pobj band,to
R3718 T5525 T5524 amod single,band
R3719 T5526 T5520 punct .,gave
R372 T857 T858 compound coli,DnaB
R3720 T5528 T5529 det These,findings
R3721 T5529 T5530 nsubj findings,indicate
R3722 T5531 T5532 mark that,loaded
R3723 T5532 T5530 ccomp loaded,indicate
R3724 T5533 T5534 det the,complex
R3725 T5534 T5532 nsubjpass complex,loaded
R3726 T5535 T5534 nmod Mcm4,complex
R3727 T5536 T5535 punct /,Mcm4
R3728 T5537 T5535 nummod 6,Mcm4
R3729 T5538 T5535 punct /,Mcm4
R373 T858 T847 conj DnaB,antigen
R3730 T5539 T5535 nummod 7,Mcm4
R3731 T5540 T5532 auxpass is,loaded
R3732 T5541 T5532 prep onto,loaded
R3733 T5542 T5541 pobj DNA,onto
R3734 T5543 T5532 advmod preferentially,loaded
R3735 T5544 T5543 cc and,preferentially
R3736 T5545 T5543 conj efficiently,preferentially
R3737 T5546 T5532 prep through,loaded
R3738 T5547 T5548 amod single,stranded
R3739 T5548 T5550 amod stranded,region
R374 T859 T860 punct (,7
R3740 T5549 T5548 punct -,stranded
R3741 T5550 T5546 pobj region,through
R3742 T5551 T5532 punct ", ",loaded
R3743 T5552 T5532 advmod regardless,loaded
R3744 T5553 T5552 prep of,regardless
R3745 T5554 T5555 det the,presence
R3746 T5555 T5553 pobj presence,of
R3747 T5556 T5555 prep of,presence
R3748 T5557 T5558 nummod 3,terminus
R3749 T5558 T5556 pobj terminus,of
R375 T860 T858 parataxis 7,DnaB
R3750 T5559 T5557 punct ′,3
R3751 T5560 T5557 punct -,3
R3752 T5561 T5557 cc or,3
R3753 T5562 T5557 conj 5,3
R3754 T5563 T5562 punct ′,5
R3755 T5564 T5558 punct -,terminus
R3756 T5565 T5530 punct .,indicate
R3757 T5567 T5568 prep In,unwound
R3758 T5569 T5567 pobj contrast,In
R3759 T5570 T5568 punct ", ",unwound
R376 T861 T860 punct ),7
R3760 T5571 T5572 advmod only,substrate
R3761 T5572 T5568 nsubjpass substrate,unwound
R3762 T5573 T5572 det the,substrate
R3763 T5574 T5572 acl containing,substrate
R3764 T5575 T5576 det a,tail
R3765 T5576 T5574 dobj tail,containing
R3766 T5577 T5576 nummod 3,tail
R3767 T5578 T5577 punct ′,3
R3768 T5579 T5580 amod single,stranded
R3769 T5580 T5576 amod stranded,tail
R377 T862 T858 cc and,DnaB
R3770 T5581 T5580 punct -,stranded
R3771 T5582 T5576 compound DNA,tail
R3772 T5583 T5584 punct (,fork
R3773 T5584 T5572 appos fork,substrate
R3774 T5585 T5584 compound A,fork
R3775 T5586 T5584 punct -,fork
R3776 T5587 T5584 punct [,fork
R3777 T5588 T5584 nummod 5,fork
R3778 T5589 T5584 punct ′,fork
R3779 T5590 T5568 punct ],unwound
R378 T863 T864 amod Archaeal,Mcm
R3780 T5591 T5568 punct ),unwound
R3781 T5592 T5568 auxpass was,unwound
R3782 T5593 T5568 advmod efficiently,unwound
R3783 T5594 T5568 cc and,unwound
R3784 T5595 T5596 compound A,fork
R3785 T5596 T5598 nsubjpass fork,unwound
R3786 T5597 T5596 punct -,fork
R3787 T5598 T5568 conj unwound,unwound
R3788 T5599 T5596 punct [,fork
R3789 T5600 T5596 nummod 3,fork
R379 T864 T858 conj Mcm,DnaB
R3790 T5601 T5596 punct ′,fork
R3791 T5602 T5596 punct ],fork
R3792 T5603 T5596 cc or,fork
R3793 T5604 T5605 compound A,fork
R3794 T5605 T5596 conj fork,fork
R3795 T5606 T5605 punct -,fork
R3796 T5607 T5605 punct [,fork
R3797 T5608 T5605 appos 3,fork
R3798 T5609 T5608 punct ′,3
R3799 T5610 T5608 punct ",",3
R380 T865 T866 punct (,9
R3800 T5611 T5608 appos 5,3
R3801 T5612 T5611 punct ′,5
R3802 T5613 T5598 punct ],unwound
R3803 T5614 T5598 punct ", ",unwound
R3804 T5615 T5598 auxpass was,unwound
R3805 T5616 T5598 neg not,unwound
R3806 T5617 T5598 agent by,unwound
R3807 T5618 T5617 pobj Mcm4,by
R3808 T5619 T5618 punct /,Mcm4
R3809 T5620 T5618 nummod 6,Mcm4
R381 T866 T864 parataxis 9,Mcm
R3810 T5621 T5618 punct /,Mcm4
R3811 T5622 T5618 nummod 7,Mcm4
R3812 T5623 T5624 punct (,4B
R3813 T5624 T5598 parataxis 4B,unwound
R3814 T5625 T5624 compound Figure,4B
R3815 T5626 T5624 punct ),4B
R3816 T5627 T5568 punct .,unwound
R3817 T5629 T5630 nsubjpass Absence,expected
R3818 T5631 T5629 prep of,Absence
R3819 T5632 T5633 compound helicase,actions
R382 T867 T866 nummod 4,9
R3820 T5633 T5631 pobj actions,of
R3821 T5634 T5629 prep on,Absence
R3822 T5635 T5636 compound A,fork
R3823 T5636 T5634 pobj fork,on
R3824 T5637 T5636 punct -,fork
R3825 T5638 T5636 punct [,fork
R3826 T5639 T5636 appos 3,fork
R3827 T5640 T5639 punct ′,3
R3828 T5641 T5639 punct ",",3
R3829 T5642 T5639 appos 5,3
R383 T868 T866 punct ",",9
R3830 T5643 T5642 punct ′,5
R3831 T5644 T5636 punct ],fork
R3832 T5645 T5636 acl composed,fork
R3833 T5646 T5647 advmod only,of
R3834 T5647 T5645 prep of,composed
R3835 T5648 T5649 compound duplex,regions
R3836 T5649 T5647 pobj regions,of
R3837 T5650 T5630 auxpass is,expected
R3838 T5651 T5652 mark since,is
R3839 T5652 T5630 advcl is,expected
R384 T869 T866 nummod 8,9
R3840 T5653 T5654 amod double,stranded
R3841 T5654 T5656 amod stranded,DNA
R3842 T5655 T5654 punct -,stranded
R3843 T5656 T5652 nsubj DNA,is
R3844 T5657 T5652 neg not,is
R3845 T5658 T5652 acomp able,is
R3846 T5659 T5660 aux to,activate
R3847 T5660 T5658 xcomp activate,able
R3848 T5661 T5662 det the,activity
R3849 T5662 T5660 dobj activity,activate
R385 T870 T866 punct ",",9
R3850 T5663 T5662 compound ATPase,activity
R3851 T5664 T5662 prep of,activity
R3852 T5665 T5664 pobj Mcm4,of
R3853 T5666 T5665 punct /,Mcm4
R3854 T5667 T5665 nummod 6,Mcm4
R3855 T5668 T5665 punct /,Mcm4
R3856 T5669 T5665 nummod 7,Mcm4
R3857 T5670 T5671 punct (,10
R3858 T5671 T5652 parataxis 10,is
R3859 T5672 T5671 punct ),10
R386 T871 T866 punct ),9
R3860 T5673 T5630 punct .,expected
R3861 T5675 T5676 prep In,binds
R3862 T5677 T5675 pobj contrast,In
R3863 T5678 T5676 punct ", ",binds
R3864 T5679 T5680 det the,PriA
R3865 T5680 T5676 nsubj PriA,binds
R3866 T5681 T5680 punct ", ",PriA
R3867 T5682 T5683 det a,helicase
R3868 T5683 T5680 appos helicase,PriA
R3869 T5684 T5685 npadvmod structure,specific
R387 T872 T835 punct ", ",composed
R3870 T5685 T5683 amod specific,helicase
R3871 T5686 T5685 punct -,specific
R3872 T5687 T5683 acl used,helicase
R3873 T5688 T5687 prep as,used
R3874 T5689 T5690 det a,control
R3875 T5690 T5688 pobj control,as
R3876 T5691 T5676 punct ", ",binds
R3877 T5692 T5676 prep at,binds
R3878 T5693 T5694 det the,junction
R3879 T5694 T5692 pobj junction,at
R388 T873 T835 auxpass are,composed
R3880 T5695 T5694 compound fork,junction
R3881 T5696 T5676 cc and,binds
R3882 T5697 T5676 conj unwinds,binds
R3883 T5698 T5699 det the,fork
R3884 T5699 T5697 dobj fork,unwinds
R3885 T5700 T5699 cc and,fork
R3886 T5701 T5702 det the,DNA
R3887 T5702 T5699 conj DNA,fork
R3888 T5703 T5702 amod nascent,DNA
R3889 T5704 T5702 amod lagging,DNA
R389 T875 T835 prep of,composed
R3890 T5705 T5702 compound strand,DNA
R3891 T5706 T5702 prep on,DNA
R3892 T5707 T5708 compound A,fork
R3893 T5708 T5706 pobj fork,on
R3894 T5709 T5708 punct -,fork
R3895 T5710 T5708 punct [,fork
R3896 T5711 T5708 nummod 5,fork
R3897 T5712 T5708 punct ′,fork
R3898 T5713 T5676 punct ],binds
R3899 T5714 T5676 punct ", ",binds
R390 T876 T877 det a,subunit
R3900 T5715 T5676 cc or,binds
R3901 T5716 T5676 conj unwinds,binds
R3902 T5717 T5718 det the,strand
R3903 T5718 T5716 dobj strand,unwinds
R3904 T5719 T5718 amod nascent,strand
R3905 T5720 T5718 amod lagging,strand
R3906 T5721 T5716 prep on,unwinds
R3907 T5722 T5723 compound A,fork
R3908 T5723 T5721 pobj fork,on
R3909 T5724 T5723 punct -,fork
R391 T877 T875 pobj subunit,of
R3910 T5725 T5723 punct [,fork
R3911 T5726 T5723 appos 3,fork
R3912 T5727 T5726 punct ′,3
R3913 T5728 T5726 punct ",",3
R3914 T5729 T5726 appos 5,3
R3915 T5730 T5729 punct ′,5
R3916 T5731 T5676 punct ],binds
R3917 T5732 T5676 punct ", ",binds
R3918 T5733 T5734 mark as,reported
R3919 T5734 T5676 advcl reported,binds
R392 T878 T877 amod single,subunit
R3920 T5735 T5734 advmod before,reported
R3921 T5736 T5737 punct (,25
R3922 T5737 T5734 parataxis 25,reported
R3923 T5738 T5737 punct ),25
R3924 T5739 T5676 punct .,binds
R3925 T5741 T5742 det These,results
R3926 T5742 T5743 nsubj results,indicate
R3927 T5744 T5745 mark that,binds
R3928 T5745 T5743 advcl binds,indicate
R3929 T5746 T5747 det the,complex
R393 T879 T874 punct ", ",composed
R3930 T5747 T5745 nsubj complex,binds
R3931 T5748 T5747 nmod Mcm4,complex
R3932 T5749 T5748 punct /,Mcm4
R3933 T5750 T5748 nummod 6,Mcm4
R3934 T5751 T5748 punct /,Mcm4
R3935 T5752 T5748 nummod 7,Mcm4
R3936 T5753 T5745 prep to,binds
R3937 T5754 T5755 amod single,stranded
R3938 T5755 T5757 amod stranded,DNA
R3939 T5756 T5755 punct -,stranded
R394 T880 T881 det the,helicases
R3940 T5757 T5753 pobj DNA,to
R3941 T5758 T5745 cc and,binds
R3942 T5759 T5760 mark that,activated
R3943 T5760 T5745 conj activated,binds
R3944 T5761 T5762 poss its,helicase
R3945 T5762 T5760 nsubjpass helicase,activated
R3946 T5763 T5760 auxpass is,activated
R3947 T5764 T5765 advmod only,in
R3948 T5765 T5760 prep in,activated
R3949 T5766 T5767 det the,presence
R395 T881 T874 nsubjpass helicases,composed
R3950 T5767 T5765 pobj presence,in
R3951 T5768 T5767 prep of,presence
R3952 T5769 T5770 det a,tail
R3953 T5770 T5768 pobj tail,of
R3954 T5771 T5772 amod single,stranded
R3955 T5772 T5770 amod stranded,tail
R3956 T5773 T5772 punct -,stranded
R3957 T5774 T5770 nummod 3,tail
R3958 T5775 T5774 punct ′,3
R3959 T5776 T5770 punct -,tail
R396 T882 T881 amod active,helicases
R3960 T5777 T5743 punct .,indicate
R3967 T5933 T5934 nummod 3,extension
R3968 T5934 T5937 nsubjpass extension,displaced
R3969 T5935 T5933 punct ′,3
R397 T883 T881 amod eukaryotic,helicases
R3970 T5936 T5934 punct -,extension
R3971 T5938 T5934 prep with,extension
R3972 T5939 T5940 nmod poly,dT
R3973 T5940 T5942 nmod dT,tail
R3974 T5941 T5940 punct (,dT
R3975 T5942 T5938 pobj tail,with
R3976 T5943 T5942 punct ),tail
R3977 T5944 T5937 auxpass is,displaced
R3978 T5945 T5937 agent by,displaced
R3979 T5946 T5945 pobj Mcm4,by
R398 T884 T881 compound Mcm,helicases
R3980 T5947 T5946 punct /,Mcm4
R3981 T5948 T5946 nummod 6,Mcm4
R3982 T5949 T5946 punct /,Mcm4
R3983 T5950 T5946 nummod 7,Mcm4
R3984 T5952 T5953 nsubjpass It,reported
R3985 T5954 T5953 aux has,reported
R3986 T5955 T5953 auxpass been,reported
R3987 T5956 T5957 mark that,unwound
R3988 T5957 T5953 ccomp unwound,reported
R3989 T5958 T5959 det the,extension
R399 T885 T874 auxpass are,composed
R3990 T5959 T5957 nsubj extension,unwound
R3991 T5960 T5959 nummod 3,extension
R3992 T5961 T5960 punct ′,3
R3993 T5962 T5959 punct -,extension
R3994 T5963 T5957 aux can,unwound
R3995 T5964 T5957 aux be,unwound
R3996 T5965 T5957 agent by,unwound
R3997 T5966 T5967 det the,homohexamer
R3998 T5967 T5965 pobj homohexamer,by
R3999 T5968 T5967 amod archaeal,homohexamer
R400 T886 T874 prep of,composed
R4000 T5969 T5967 compound Mcm,homohexamer
R4001 T5970 T5957 cc but,unwound
R4002 T5971 T5957 conj not,unwound
R4003 T5972 T5971 agent by,not
R4004 T5973 T5974 nmod fission,complexes
R4005 T5974 T5972 pobj complexes,by
R4006 T5975 T5973 cc and,fission
R4007 T5976 T5973 conj budding,fission
R4008 T5977 T5974 compound yeast,complexes
R4009 T5978 T5974 compound Mcm,complexes
R401 T887 T888 nummod two,proteins
R4010 T5979 T5980 punct (,27
R4011 T5980 T5953 parataxis 27,reported
R4012 T5981 T5980 nummod 14,27
R4013 T5982 T5980 punct ",",27
R4014 T5983 T5980 nummod 26,27
R4015 T5984 T5980 punct ",",27
R4016 T5985 T5980 punct ),27
R4017 T5986 T5953 punct .,reported
R4018 T5988 T5989 nsubj We,constructed
R4019 T5990 T5989 aux have,constructed
R402 T888 T886 pobj proteins,of
R4020 T5991 T5992 nummod 3,extension
R4021 T5992 T5995 compound extension,substrates
R4022 T5993 T5991 punct ′,3
R4023 T5994 T5992 punct -,extension
R4024 T5995 T5989 dobj substrates,constructed
R4025 T5996 T5995 acl containing,substrates
R4026 T5997 T5998 nmod poly,dT
R4027 T5998 T6000 nmod dT,tails
R4028 T5999 T5998 punct (,dT
R4029 T6000 T5996 dobj tails,containing
R403 T889 T888 amod trimeric,proteins
R4030 T6001 T5998 punct ),dT
R4031 T6002 T5998 punct ", ",dT
R4032 T6003 T6004 nmod poly,dA
R4033 T6004 T5998 conj dA,dT
R4034 T6005 T6004 punct (,dA
R4035 T6006 T6004 punct ),dA
R4036 T6007 T6004 punct ", ",dA
R4037 T6008 T6009 nmod poly,dC
R4038 T6009 T6004 conj dC,dA
R4039 T6010 T6009 punct (,dC
R404 T890 T888 nmod Mcm4,proteins
R4040 T6011 T6009 punct ),dC
R4041 T6012 T6009 cc and,dC
R4042 T6013 T6014 nmod poly,dG
R4043 T6014 T6009 conj dG,dC
R4044 T6015 T6014 punct (,dG
R4045 T6016 T6014 punct ),dG
R4046 T6017 T6000 nummod 3,tails
R4047 T6018 T6017 punct ′,3
R4048 T6019 T6000 punct -,tails
R4049 T6020 T5989 prep in,constructed
R405 T891 T890 punct /,Mcm4
R4050 T6021 T6020 pobj order,in
R4051 T6022 T6023 aux to,examine
R4052 T6023 T6021 acl examine,order
R4053 T6024 T6025 mark whether,displace
R4054 T6025 T6023 ccomp displace,examine
R4055 T6026 T6027 compound mouse,Mcm4
R4056 T6027 T6025 nsubj Mcm4,displace
R4057 T6028 T6027 punct /,Mcm4
R4058 T6029 T6027 nummod 6,Mcm4
R4059 T6030 T6027 punct /,Mcm4
R406 T892 T890 nummod 6,Mcm4
R4060 T6031 T6027 nummod 7,Mcm4
R4061 T6032 T6025 aux can,displace
R4062 T6033 T6034 det these,substrates
R4063 T6034 T6025 dobj substrates,displace
R4064 T6035 T5989 punct .,constructed
R4065 T6037 T6038 nsubj We,found
R4066 T6039 T6038 advmod first,found
R4067 T6040 T6041 mark that,binds
R4068 T6041 T6038 ccomp binds,found
R4069 T6042 T6041 nsubj Mcm4,binds
R407 T893 T890 punct /,Mcm4
R4070 T6043 T6042 punct /,Mcm4
R4071 T6044 T6042 nummod 6,Mcm4
R4072 T6045 T6042 punct /,Mcm4
R4073 T6046 T6042 nummod 7,Mcm4
R4074 T6047 T6041 prep to,binds
R4075 T6048 T6049 nmod dT,extension
R4076 T6049 T6047 pobj extension,to
R4077 T6050 T6048 punct -,dT
R4078 T6051 T6048 punct ", ",dT
R4079 T6052 T6048 conj dC,dT
R408 T894 T890 nummod 7,Mcm4
R4080 T6053 T6052 punct -,dC
R4081 T6054 T6052 cc and,dC
R4082 T6055 T6052 conj dG,dC
R4083 T6056 T6049 punct -,extension
R4084 T6057 T6049 nummod 3,extension
R4085 T6058 T6057 punct ′,3
R4086 T6059 T6049 punct -,extension
R4087 T6060 T6041 prep with,binds
R4088 T6061 T6062 amod similar,affinity
R4089 T6062 T6060 pobj affinity,with
R409 T895 T896 punct (,10
R4090 T6063 T6041 punct ", ",binds
R4091 T6064 T6041 cc and,binds
R4092 T6065 T6041 conj to,binds
R4093 T6066 T6067 nmod dA,extension
R4094 T6067 T6065 pobj extension,to
R4095 T6068 T6067 punct -,extension
R4096 T6069 T6067 nummod 3,extension
R4097 T6070 T6069 punct ′,3
R4098 T6071 T6067 punct -,extension
R4099 T6072 T6065 prep with,to
R410 T896 T874 parataxis 10,composed
R4100 T6073 T6074 advmod much,lower
R4101 T6074 T6075 amod lower,affinity
R4102 T6075 T6072 pobj affinity,with
R4103 T6076 T6077 punct (,5A
R4104 T6077 T6065 parataxis 5A,to
R4105 T6078 T6077 compound Figure,5A
R4106 T6079 T6077 punct ),5A
R4107 T6080 T6038 punct .,found
R4108 T6082 T6083 nsubj This,is
R4109 T6084 T6083 acomp consistent,is
R411 T897 T898 punct –,14
R4110 T6085 T6084 prep with,consistent
R4111 T6086 T6087 det the,results
R4112 T6087 T6085 pobj results,with
R4113 T6088 T6087 amod previous,results
R4114 T6089 T6090 mark that,binds
R4115 T6090 T6087 acl binds,results
R4116 T6091 T6090 nsubj Mcm4,binds
R4117 T6092 T6091 punct /,Mcm4
R4118 T6093 T6091 nummod 6,Mcm4
R4119 T6094 T6091 punct /,Mcm4
R412 T898 T896 prep 14,10
R4120 T6095 T6091 nummod 7,Mcm4
R4121 T6096 T6090 prep to,binds
R4122 T6097 T6098 compound 50mer,dT
R4123 T6098 T6096 pobj dT,to
R4124 T6099 T6098 compound oligo,dT
R4125 T6100 T6098 punct -,dT
R4126 T6101 T6098 punct ", ",dT
R4127 T6102 T6103 punct -,dC
R4128 T6103 T6098 conj dC,dT
R4129 T6104 T6103 cc and,dC
R413 T899 T896 punct ),10
R4130 T6105 T6106 punct -,dG
R4131 T6106 T6103 conj dG,dC
R4132 T6107 T6096 punct ", ",to
R4133 T6108 T6096 cc but,to
R4134 T6109 T6108 neg not,but
R4135 T6110 T6096 conj to,to
R4136 T6111 T6112 det a,dA
R4137 T6112 T6110 pobj dA,to
R4138 T6113 T6112 compound 50mer,dA
R4139 T6114 T6112 compound oligo,dA
R414 T900 T874 punct .,composed
R4140 T6115 T6112 punct -,dA
R4141 T6116 T6117 punct (,13
R4142 T6117 T6083 parataxis 13,is
R4143 T6118 T6117 punct ),13
R4144 T6119 T6083 punct .,is
R4145 T6121 T6122 nsubj We,found
R4146 T6123 T6124 mark that,displaced
R4147 T6124 T6122 ccomp displaced,found
R4148 T6125 T6126 npadvmod dT,tailed
R4149 T6126 T6128 amod tailed,extension
R415 T902 T903 predet All,proteins
R4150 T6127 T6126 punct -,tailed
R4151 T6128 T6124 nsubjpass extension,displaced
R4152 T6129 T6128 nummod 3,extension
R4153 T6130 T6129 punct ′,3
R4154 T6131 T6128 punct -,extension
R4155 T6132 T6124 auxpass was,displaced
R4156 T6133 T6124 advmod efficiently,displaced
R4157 T6134 T6124 agent by,displaced
R4158 T6135 T6134 pobj Mcm,by
R4159 T6136 T6135 nummod 4,Mcm
R416 T903 T910 nsubj proteins,contain
R4160 T6137 T6135 punct /,Mcm
R4161 T6138 T6135 nummod 6,Mcm
R4162 T6139 T6135 punct /,Mcm
R4163 T6140 T6135 nummod 7,Mcm
R4164 T6141 T6142 mark while,detected
R4165 T6142 T6124 advcl detected,displaced
R4166 T6143 T6144 advmod almost,no
R4167 T6144 T6142 nsubjpass no,detected
R4168 T6145 T6144 cc or,no
R4169 T6146 T6147 advmod very,little
R417 T904 T903 det the,proteins
R4170 T6147 T6148 nmod little,activity
R4171 T6148 T6144 conj activity,no
R4172 T6149 T6142 auxpass was,detected
R4173 T6150 T6142 prep on,detected
R4174 T6151 T6152 amod other,substrates
R4175 T6152 T6150 pobj substrates,on
R4176 T6153 T6154 punct (,5B
R4177 T6154 T6142 parataxis 5B,detected
R4178 T6155 T6154 compound Figure,5B
R4179 T6156 T6154 cc and,5B
R418 T905 T903 nummod six,proteins
R4180 T6157 T6154 conj C,5B
R4181 T6158 T6154 punct ),5B
R4182 T6159 T6122 punct .,found
R4183 T6161 T6162 nsubj This,is
R4184 T6163 T6162 prep in,is
R4185 T6164 T6165 amod sharp,contrast
R4186 T6165 T6163 pobj contrast,in
R4187 T6166 T6165 prep to,contrast
R4188 T6167 T6168 nmod yeast,helicases
R4189 T6168 T6166 pobj helicases,to
R419 T906 T907 nmod Mcm,2
R4190 T6169 T6168 nmod Mcm4,helicases
R4191 T6170 T6169 punct /,Mcm4
R4192 T6171 T6169 nummod 6,Mcm4
R4193 T6172 T6169 punct /,Mcm4
R4194 T6173 T6169 nummod 7,Mcm4
R4195 T6174 T6175 dep which,are
R4196 T6175 T6168 relcl are,helicases
R4197 T6176 T6175 neg not,are
R4198 T6177 T6175 acomp capable,are
R4199 T6178 T6177 prep of,capable
R420 T907 T903 nummod 2,proteins
R4200 T6179 T6178 pcomp unwinding,of
R4201 T6180 T6181 nummod 3,extension
R4202 T6181 T6184 compound extension,substrates
R4203 T6182 T6180 punct ′,3
R4204 T6183 T6181 punct -,extension
R4205 T6184 T6179 dobj substrates,unwinding
R4206 T6185 T6162 punct .,is
R4207 T6187 T6188 advmod Furthermore,displaced
R4208 T6189 T6188 punct ", ",displaced
R4209 T6190 T6191 npadvmod dT,tailed
R421 T908 T909 punct –,7
R4210 T6191 T6193 amod tailed,extension
R4211 T6192 T6191 punct -,tailed
R4212 T6193 T6188 nsubjpass extension,displaced
R4213 T6194 T6193 nummod 5,extension
R4214 T6195 T6194 punct ′,5
R4215 T6196 T6193 punct -,extension
R4216 T6197 T6193 punct ", ",extension
R4217 T6198 T6199 prep to,bind
R4218 T6199 T6193 relcl bind,extension
R4219 T6200 T6198 pobj which,to
R422 T909 T907 prep 7,2
R4220 T6201 T6199 nsubj Mcm4,bind
R4221 T6202 T6201 punct /,Mcm4
R4222 T6203 T6201 nummod 6,Mcm4
R4223 T6204 T6201 punct /,Mcm4
R4224 T6205 T6201 nummod 7,Mcm4
R4225 T6206 T6199 aux could,bind
R4226 T6207 T6188 punct ", ",displaced
R4227 T6208 T6188 auxpass was,displaced
R4228 T6209 T6188 neg not,displaced
R4229 T6210 T6188 agent by,displaced
R423 T911 T912 advmod highly,conserved
R4230 T6211 T6212 det the,helicase
R4231 T6212 T6210 pobj helicase,by
R4232 T6213 T6212 compound Mcm,helicase
R4233 T6214 T6188 punct .,displaced
R4234 T6216 T6217 det The,addition
R4235 T6217 T6218 nsubj addition,stimulated
R4236 T6219 T6217 prep of,addition
R4237 T6220 T6221 det the,tail
R4238 T6221 T6219 pobj tail,of
R4239 T6222 T6221 nummod 5,tail
R424 T912 T913 amod conserved,motifs
R4240 T6223 T6222 punct ′,5
R4241 T6224 T6221 punct -,tail
R4242 T6225 T6217 prep to,addition
R4243 T6226 T6227 npadvmod dT,tailed
R4244 T6227 T6229 amod tailed,extension
R4245 T6228 T6227 punct -,tailed
R4246 T6229 T6225 pobj extension,to
R4247 T6230 T6229 nummod 3,extension
R4248 T6231 T6230 punct ′,3
R4249 T6232 T6229 punct -,extension
R425 T913 T910 dobj motifs,contain
R4250 T6233 T6218 advmod further,stimulated
R4251 T6234 T6235 det the,displacement
R4252 T6235 T6218 dobj displacement,stimulated
R4253 T6236 T6237 punct (,5D
R4254 T6237 T6235 parataxis 5D,displacement
R4255 T6238 T6237 compound Figure,5D
R4256 T6239 T6237 punct ),5D
R4257 T6240 T6218 punct ", ",stimulated
R4258 T6241 T6218 advcl confirming,stimulated
R4259 T6242 T6243 det the,report
R426 T914 T915 npadvmod DNA,dependent
R4260 T6243 T6241 dobj report,confirming
R4261 T6244 T6243 amod previous,report
R4262 T6245 T6246 mark that,facilitate
R4263 T6246 T6243 acl facilitate,report
R4264 T6247 T6248 det the,structures
R4265 T6248 T6246 nsubj structures,facilitate
R4266 T6249 T6248 compound fork,structures
R4267 T6250 T6251 det the,action
R4268 T6251 T6246 dobj action,facilitate
R4269 T6252 T6251 compound helicase,action
R427 T915 T913 amod dependent,motifs
R4270 T6253 T6218 punct .,stimulated
R4271 T6255 T6256 advmod Thus,strengthen
R4272 T6257 T6256 punct ", ",strengthen
R4273 T6258 T6259 det these,results
R4274 T6259 T6256 nsubj results,strengthen
R4275 T6260 T6256 advmod further,strengthen
R4276 T6261 T6262 poss our,conclusion
R4277 T6262 T6256 dobj conclusion,strengthen
R4278 T6263 T6264 mark that,activated
R4279 T6264 T6262 acl activated,conclusion
R428 T916 T915 punct -,dependent
R4280 T6265 T6266 det the,helicase
R4281 T6266 T6264 nsubjpass helicase,activated
R4282 T6267 T6266 amod mammalian,helicase
R4283 T6268 T6266 compound Mcm,helicase
R4284 T6269 T6264 auxpass is,activated
R4285 T6270 T6264 advmod generally,activated
R4286 T6271 T6264 agent by,activated
R4287 T6272 T6273 det the,stretch
R4288 T6273 T6271 pobj stretch,by
R4289 T6274 T6273 compound thymine,stretch
R429 T917 T913 compound ATPase,motifs
R4290 T6275 T6273 punct -,stretch
R4291 T6276 T6273 prep on,stretch
R4292 T6277 T6278 det the,tail
R4293 T6278 T6276 pobj tail,on
R4294 T6279 T6278 nummod 3,tail
R4295 T6280 T6279 punct ′,3
R4296 T6281 T6278 punct -,tail
R4297 T6282 T6283 amod single,stranded
R4298 T6283 T6278 amod stranded,tail
R4299 T6284 T6283 punct -,stranded
R430 T918 T910 prep in,contain
R4300 T6285 T6256 punct .,strengthen
R4301 T6404 T6405 det The,effects
R4302 T6406 T6405 prep of,effects
R4303 T6407 T6408 det the,content
R4304 T6408 T6406 pobj content,of
R4305 T6409 T6408 compound thymine,content
R4306 T6410 T6408 prep of,content
R4307 T6411 T6412 det the,tail
R4308 T6412 T6410 pobj tail,of
R4309 T6413 T6412 nummod 3,tail
R431 T919 T920 poss their,regions
R4310 T6414 T6413 punct ′,3
R4311 T6415 T6412 punct -,tail
R4312 T6416 T6405 prep on,effects
R4313 T6417 T6418 compound Mcm,helicase
R4314 T6418 T6419 compound helicase,activity
R4315 T6419 T6416 pobj activity,on
R4316 T6421 T6422 nsubj We,examined
R4317 T6423 T6422 advmod next,examined
R4318 T6424 T6425 advmod more,systematically
R4319 T6425 T6422 advmod systematically,examined
R432 T920 T918 pobj regions,in
R4320 T6426 T6427 det the,effect
R4321 T6427 T6422 dobj effect,examined
R4322 T6428 T6427 prep of,effect
R4323 T6429 T6430 det the,compositions
R4324 T6430 T6428 pobj compositions,of
R4325 T6431 T6430 prep of,compositions
R4326 T6432 T6433 det the,residues
R4327 T6433 T6431 pobj residues,of
R4328 T6434 T6433 compound thymine,residues
R4329 T6435 T6433 prep of,residues
R433 T921 T920 amod central,regions
R4330 T6436 T6437 det the,tail
R4331 T6437 T6435 pobj tail,of
R4332 T6438 T6437 nummod 3,tail
R4333 T6439 T6438 punct ′,3
R4334 T6440 T6437 punct -,tail
R4335 T6441 T6427 prep on,effect
R4336 T6442 T6443 det the,activity
R4337 T6443 T6441 pobj activity,on
R4338 T6444 T6445 compound Mcm,helicase
R4339 T6445 T6443 compound helicase,activity
R434 T922 T923 punct (,16
R4340 T6446 T6422 punct .,examined
R4341 T6448 T6449 det A,DNA
R4342 T6449 T6456 nsubjpass DNA,constructed
R4343 T6450 T6449 amod partial,DNA
R4344 T6451 T6449 nmod heteroduplex,DNA
R4345 T6452 T6449 nmod M13,DNA
R4346 T6453 T6454 amod single,stranded
R4347 T6454 T6449 amod stranded,DNA
R4348 T6455 T6454 punct -,stranded
R4349 T6457 T6449 acl containing,DNA
R435 T923 T910 parataxis 16,contain
R4350 T6458 T6459 det a,region
R4351 T6459 T6457 dobj region,containing
R4352 T6460 T6461 nummod 37,bp
R4353 T6461 T6459 compound bp,region
R4354 T6462 T6459 compound duplex,region
R4355 T6463 T6457 prep in,containing
R4356 T6464 T6465 det the,presence
R4357 T6465 T6463 pobj presence,in
R4358 T6466 T6465 cc or,presence
R4359 T6467 T6465 conj absence,presence
R436 T924 T923 nummod 15,16
R4360 T6468 T6465 prep of,presence
R4361 T6469 T6470 det the,tail
R4362 T6470 T6468 pobj tail,of
R4363 T6471 T6472 nummod 50,nt
R4364 T6472 T6473 npadvmod nt,long
R4365 T6473 T6470 amod long,tail
R4366 T6474 T6470 nummod 3,tail
R4367 T6475 T6474 punct ′,3
R4368 T6476 T6470 punct -,tail
R4369 T6477 T6470 prep of,tail
R437 T925 T923 punct ",",16
R4370 T6478 T6479 amod various,compositions
R4371 T6479 T6477 pobj compositions,of
R4372 T6480 T6479 compound nucleotide,compositions
R4373 T6481 T6456 auxpass were,constructed
R4374 T6482 T6456 cc and,constructed
R4375 T6483 T6484 det the,unwinding
R4376 T6484 T6485 nsubjpass unwinding,examined
R4377 T6485 T6456 conj examined,constructed
R4378 T6486 T6484 prep by,unwinding
R4379 T6487 T6488 compound mouse,Mcm4
R438 T926 T923 punct ),16
R4380 T6488 T6486 pobj Mcm4,by
R4381 T6489 T6488 punct /,Mcm4
R4382 T6490 T6488 nummod 6,Mcm4
R4383 T6491 T6488 punct /,Mcm4
R4384 T6492 T6488 nummod 7,Mcm4
R4385 T6493 T6485 auxpass was,examined
R4386 T6494 T6495 punct (,6A
R4387 T6495 T6485 parataxis 6A,examined
R4388 T6496 T6495 compound Figure,6A
R4389 T6497 T6495 punct ),6A
R439 T927 T910 punct .,contain
R4390 T6498 T6485 punct .,examined
R4391 T6500 T6501 prep Due,displaced
R4392 T6502 T6500 pcomp to,Due
R4393 T6503 T6504 poss its,processivity
R4394 T6504 T6500 pobj processivity,Due
R4395 T6505 T6504 amod low,processivity
R4396 T6506 T6501 punct ", ",displaced
R4397 T6507 T6501 nsubj Mcm4,displaced
R4398 T6508 T6507 punct /,Mcm4
R4399 T6509 T6507 nummod 6,Mcm4
R440 T929 T930 prep Among,shown
R4400 T6510 T6507 punct /,Mcm4
R4401 T6511 T6507 nummod 7,Mcm4
R4402 T6512 T6501 advmod barely,displaced
R4403 T6513 T6514 det the,oligonucleotide
R4404 T6514 T6501 dobj oligonucleotide,displaced
R4405 T6515 T6514 amod annealed,oligonucleotide
R4406 T6516 T6514 compound 37mer,oligonucleotide
R4407 T6517 T6514 prep without,oligonucleotide
R4408 T6518 T6519 det a,tail
R4409 T6519 T6517 pobj tail,without
R441 T931 T932 det the,subcomplexes
R4410 T6520 T6519 nummod 3,tail
R4411 T6521 T6520 punct ′,3
R4412 T6522 T6519 punct -,tail
R4413 T6523 T6524 punct (,6A
R4414 T6524 T6514 parataxis 6A,oligonucleotide
R4415 T6525 T6524 dep 37mer,6A
R4416 T6526 T6524 punct ;,6A
R4417 T6527 T6524 compound Figure,6A
R4418 T6528 T6524 punct ),6A
R4419 T6529 T6501 punct .,displaced
R442 T932 T929 pobj subcomplexes,Among
R4420 T6531 T6532 advmod Only,activity
R4421 T6532 T6535 nsubjpass activity,observed
R4422 T6533 T6532 amod weak,activity
R4423 T6534 T6532 compound helicase,activity
R4424 T6536 T6535 auxpass was,observed
R4425 T6537 T6535 prep on,observed
R4426 T6538 T6539 det the,substrate
R4427 T6539 T6537 pobj substrate,on
R4428 T6540 T6539 acl carrying,substrate
R4429 T6541 T6542 det the,tail
R443 T933 T932 amod several,subcomplexes
R4430 T6542 T6540 dobj tail,carrying
R4431 T6543 T6542 nummod 3,tail
R4432 T6544 T6543 punct ′,3
R4433 T6545 T6542 punct -,tail
R4434 T6546 T6542 prep of,tail
R4435 T6547 T6548 nummod 25,repeats
R4436 T6548 T6546 pobj repeats,of
R4437 T6549 T6548 prep of,repeats
R4438 T6550 T6551 compound TA,dinucleotide
R4439 T6551 T6549 pobj dinucleotide,of
R444 T934 T932 amod stable,subcomplexes
R4440 T6552 T6553 punct (,6A
R4441 T6553 T6540 parataxis 6A,carrying
R4442 T6554 T6555 nummod 37,TA25
R4443 T6555 T6553 dep TA25,6A
R4444 T6556 T6555 punct -,TA25
R4445 T6557 T6553 punct ;,6A
R4446 T6558 T6553 compound Figure,6A
R4447 T6559 T6553 punct ),6A
R4448 T6560 T6535 punct ", ",observed
R4449 T6561 T6562 mark whereas,observed
R445 T935 T936 dep which,generate
R4450 T6562 T6535 advcl observed,observed
R4451 T6563 T6564 amod strong,activity
R4452 T6564 T6562 nsubjpass activity,observed
R4453 T6565 T6564 compound helicase,activity
R4454 T6566 T6562 auxpass was,observed
R4455 T6567 T6562 prep on,observed
R4456 T6568 T6569 nummod 37,TTA17
R4457 T6569 T6567 pobj TTA17,on
R4458 T6570 T6569 punct -,TTA17
R4459 T6571 T6535 punct .,observed
R446 T936 T932 relcl generate,subcomplexes
R4460 T6573 T6574 nsubj Increase,increase
R4461 T6575 T6573 prep of,Increase
R4462 T6576 T6577 compound thymine,content
R4463 T6577 T6575 pobj content,of
R4464 T6578 T6573 prep on,Increase
R4465 T6579 T6580 det the,tail
R4466 T6580 T6578 pobj tail,on
R4467 T6581 T6580 nummod 3,tail
R4468 T6582 T6581 punct ′,3
R4469 T6583 T6580 punct -,tail
R447 T937 T938 compound MCM,proteins
R4470 T6584 T6574 aux did,increase
R4471 T6585 T6574 neg not,increase
R4472 T6586 T6574 advmod further,increase
R4473 T6587 T6588 det the,extent
R4474 T6588 T6574 dobj extent,increase
R4475 T6589 T6588 prep of,extent
R4476 T6590 T6589 pobj unwinding,of
R4477 T6591 T6592 punct (,6A
R4478 T6592 T6590 parataxis 6A,unwinding
R4479 T6593 T6594 nummod 37,TTTA13
R448 T938 T936 nsubj proteins,generate
R4480 T6594 T6592 dep TTTA13,6A
R4481 T6595 T6594 punct -,TTTA13
R4482 T6596 T6594 punct ", ",TTTA13
R4483 T6597 T6598 nummod 37,TTTTA10
R4484 T6598 T6594 conj TTTTA10,TTTA13
R4485 T6599 T6598 punct -,TTTTA10
R4486 T6600 T6598 cc and,TTTTA10
R4487 T6601 T6602 nummod 37,T50
R4488 T6602 T6598 conj T50,TTTTA10
R4489 T6603 T6602 punct -,T50
R449 T939 T936 aux can,generate
R4490 T6604 T6592 punct ;,6A
R4491 T6605 T6592 compound Figure,6A
R4492 T6606 T6592 punct ),6A
R4493 T6607 T6574 punct ", ",increase
R4494 T6608 T6574 advcl suggesting,increase
R4495 T6609 T6610 mark that,be
R4496 T6610 T6608 ccomp be,suggesting
R4497 T6611 T6612 nummod 17,repeats
R4498 T6612 T6610 nsubj repeats,be
R4499 T6613 T6612 prep of,repeats
R450 T940 T930 punct ", ",shown
R4500 T6614 T6615 compound TT,dinucleotides
R4501 T6615 T6613 pobj dinucleotides,of
R4502 T6616 T6617 punct (,thymine
R4503 T6617 T6615 parataxis thymine,dinucleotides
R4504 T6618 T6619 nummod 67,%
R4505 T6619 T6617 compound %,thymine
R4506 T6620 T6617 punct ),thymine
R4507 T6621 T6610 aux may,be
R4508 T6622 T6610 acomp sufficient,be
R4509 T6623 T6622 prep for,sufficient
R451 T941 T942 advmod only,complex
R4510 T6624 T6623 pobj activation,for
R4511 T6625 T6624 prep of,activation
R4512 T6626 T6627 compound Mcm,helicase
R4513 T6627 T6625 pobj helicase,of
R4514 T6628 T6574 punct .,increase
R4515 T6630 T6631 nsubjpass It,noted
R4516 T6632 T6631 aux should,noted
R4517 T6633 T6631 auxpass be,noted
R4518 T6634 T6635 mark that,anneal
R4519 T6635 T6631 ccomp anneal,noted
R452 T942 T930 nsubjpass complex,shown
R4520 T6636 T6637 det the,tail
R4521 T6637 T6635 nsubj tail,anneal
R4522 T6638 T6637 compound TA25,tail
R4523 T6639 T6635 aux would,anneal
R4524 T6640 T6635 dep self,anneal
R4525 T6641 T6635 punct -,anneal
R4526 T6642 T6635 cc and,anneal
R4527 T6643 T6644 nsubj this,prevent
R4528 T6644 T6635 conj prevent,anneal
R4529 T6645 T6644 aux would,prevent
R453 T943 T942 det the,complex
R4530 T6646 T6647 det the,loading
R4531 T6647 T6644 dobj loading,prevent
R4532 T6648 T6647 prep of,loading
R4533 T6649 T6648 pobj Mcm,of
R4534 T6650 T6631 punct ", ",noted
R4535 T6651 T6652 mark since,enhance
R4536 T6652 T6631 advcl enhance,noted
R4537 T6653 T6654 det the,tail
R4538 T6654 T6652 nsubj tail,enhance
R4539 T6655 T6654 nummod 3,tail
R454 T944 T942 nmod Mcm4,complex
R4540 T6656 T6655 punct ′,3
R4541 T6657 T6654 punct -,tail
R4542 T6658 T6654 acl containing,tail
R4543 T6659 T6658 dobj TTAA13,containing
R4544 T6660 T6659 cc or,TTAA13
R4545 T6661 T6659 conj TTTAAA8,TTAA13
R4546 T6662 T6652 aux did,enhance
R4547 T6663 T6652 neg not,enhance
R4548 T6664 T6665 det the,unwinding
R4549 T6665 T6652 dobj unwinding,enhance
R455 T945 T944 punct /,Mcm4
R4550 T6666 T6652 advmod either,enhance
R4551 T6667 T6668 punct (,shown
R4552 T6668 T6652 parataxis shown,enhance
R4553 T6669 T6668 nsubj data,shown
R4554 T6670 T6668 neg not,shown
R4555 T6671 T6668 punct ),shown
R4556 T6672 T6631 punct .,noted
R4557 T6674 T6675 advmod Therefore,examined
R4558 T6676 T6675 punct ", ",examined
R4559 T6677 T6675 nsubj we,examined
R456 T946 T944 nummod 6,Mcm4
R4560 T6678 T6675 advmod next,examined
R4561 T6679 T6680 det the,effects
R4562 T6680 T6675 dobj effects,examined
R4563 T6681 T6680 prep of,effects
R4564 T6682 T6683 amod repeating,sequences
R4565 T6683 T6681 pobj sequences,of
R4566 T6684 T6683 prep of,sequences
R4567 T6685 T6684 pobj thymine,of
R4568 T6686 T6685 cc and,thymine
R4569 T6687 T6685 conj cytosine,thymine
R457 T947 T944 punct /,Mcm4
R4570 T6688 T6689 punct (,6B
R4571 T6689 T6675 parataxis 6B,examined
R4572 T6690 T6689 compound Figure,6B
R4573 T6691 T6689 punct ),6B
R4574 T6692 T6675 punct .,examined
R4575 T6694 T6695 amod Partial,substrates
R4576 T6695 T6697 nsubjpass substrates,displaced
R4577 T6696 T6695 compound heteroduplex,substrates
R4578 T6698 T6695 acl containing,substrates
R4579 T6699 T6700 det the,tail
R458 T948 T944 nummod 7,Mcm4
R4580 T6700 T6698 dobj tail,containing
R4581 T6701 T6700 nummod 3,tail
R4582 T6702 T6701 punct ′,3
R4583 T6703 T6700 punct -,tail
R4584 T6704 T6700 prep of,tail
R4585 T6705 T6706 nmod TC25,TTCC17
R4586 T6706 T6704 pobj TTCC17,of
R4587 T6707 T6706 punct ", ",TTCC17
R4588 T6708 T6706 nmod TTCC13,TTCC17
R4589 T6709 T6706 punct ", ",TTCC17
R459 T949 T930 aux has,shown
R4590 T6710 T6706 nmod TTTCCC8,TTCC17
R4591 T6711 T6706 punct ", ",TTCC17
R4592 T6712 T6706 nmod TTTTCCCC6,TTCC17
R4593 T6713 T6706 punct ", ",TTCC17
R4594 T6714 T6697 auxpass were,displaced
R4595 T6715 T6697 agent by,displaced
R4596 T6716 T6717 det the,complex
R4597 T6717 T6715 pobj complex,by
R4598 T6718 T6717 nmod Mcm4,complex
R4599 T6719 T6718 punct /,Mcm4
R460 T950 T930 auxpass been,shown
R4600 T6720 T6718 nummod 6,Mcm4
R4601 T6721 T6718 punct /,Mcm4
R4602 T6722 T6718 nummod 7,Mcm4
R4603 T6723 T6697 prep to,displaced
R4604 T6724 T6725 det the,extent
R4605 T6725 T6723 pobj extent,to
R4606 T6726 T6725 amod similar,extent
R4607 T6727 T6726 prep to,similar
R4608 T6728 T6727 pobj that,to
R4609 T6729 T6728 acl achieved,that
R461 T951 T952 aux to,possess
R4610 T6730 T6729 agent by,achieved
R4611 T6731 T6730 pobj TTA17,by
R4612 T6732 T6697 punct ", ",displaced
R4613 T6733 T6697 advcl suggesting,displaced
R4614 T6734 T6735 mark that,is
R4615 T6735 T6733 ccomp is,suggesting
R4616 T6736 T6737 nummod 50,%
R4617 T6737 T6738 compound %,content
R4618 T6738 T6735 nsubj content,is
R4619 T6739 T6738 compound thymine,content
R462 T952 T930 xcomp possess,shown
R4620 T6740 T6735 acomp sufficient,is
R4621 T6741 T6740 prep for,sufficient
R4622 T6742 T6743 amod full,activation
R4623 T6743 T6741 pobj activation,for
R4624 T6744 T6743 prep of,activation
R4625 T6745 T6746 compound Mcm,helicase
R4626 T6746 T6744 pobj helicase,of
R4627 T6747 T6697 punct .,displaced
R4628 T6749 T6750 nsubjpass TTCC13,displaced
R4629 T6751 T6749 cc and,TTCC13
R463 T953 T954 det an,activity
R4630 T6752 T6749 conj TTCC17,TTCC13
R4631 T6753 T6750 auxpass were,displaced
R4632 T6754 T6750 prep to,displaced
R4633 T6755 T6756 det a,extent
R4634 T6756 T6754 pobj extent,to
R4635 T6757 T6756 amod similar,extent
R4636 T6758 T6750 punct ", ",displaced
R4637 T6759 T6750 advcl consistent,displaced
R4638 T6760 T6759 prep with,consistent
R4639 T6761 T6762 det the,result
R464 T954 T952 dobj activity,possess
R4640 T6762 T6760 pobj result,with
R4641 T6763 T6762 amod earlier,result
R4642 T6764 T6765 mark that,is
R4643 T6765 T6762 acl is,result
R4644 T6766 T6767 det a,tail
R4645 T6767 T6765 nsubj tail,is
R4646 T6768 T6769 nummod 40,nt
R4647 T6769 T6767 compound nt,tail
R4648 T6770 T6765 acomp sufficient,is
R4649 T6771 T6770 prep for,sufficient
R465 T955 T954 amod intrinsic,activity
R4650 T6772 T6771 pobj activation,for
R4651 T6773 T6774 punct (,13
R4652 T6774 T6765 parataxis 13,is
R4653 T6775 T6774 punct ),13
R4654 T6776 T6750 punct .,displaced
R4655 T6778 T6779 advmod However,decreased
R4656 T6780 T6779 punct ", ",decreased
R4657 T6781 T6782 det the,efficiency
R4658 T6782 T6779 nsubj efficiency,decreased
R4659 T6783 T6782 prep of,efficiency
R466 T956 T957 compound DNA,helicase
R4660 T6784 T6785 det the,displacement
R4661 T6785 T6783 pobj displacement,of
R4662 T6786 T6779 advmod significantly,decreased
R4663 T6787 T6788 mark as,dropped
R4664 T6788 T6779 advcl dropped,decreased
R4665 T6789 T6790 det the,content
R4666 T6790 T6788 nsubj content,dropped
R4667 T6791 T6790 compound thymine,content
R4668 T6792 T6790 prep of,content
R4669 T6793 T6794 det the,tail
R467 T957 T954 compound helicase,activity
R4670 T6794 T6792 pobj tail,of
R4671 T6795 T6794 nummod 3,tail
R4672 T6796 T6795 punct ′,3
R4673 T6797 T6794 punct -,tail
R4674 T6798 T6788 prep to,dropped
R4675 T6799 T6800 nummod 33,%
R4676 T6800 T6798 pobj %,to
R4677 T6801 T6788 punct ", ",dropped
R4678 T6802 T6788 prep as,dropped
R4679 T6803 T6802 prep in,as
R468 T958 T959 punct (,10
R4680 T6804 T6803 pobj TAA17,in
R4681 T6805 T6804 cc or,TAA17
R4682 T6806 T6804 conj TCC17,TAA17
R4683 T6807 T6808 punct (,6B
R4684 T6808 T6788 parataxis 6B,dropped
R4685 T6809 T6808 compound Figure,6B
R4686 T6810 T6808 cc and,6B
R4687 T6811 T6808 conj C,6B
R4688 T6812 T6808 punct ),6B
R4689 T6813 T6779 punct .,decreased
R469 T959 T930 parataxis 10,shown
R4690 T6815 T6816 advmod Thus,be
R4691 T6817 T6816 punct ", ",be
R4692 T6818 T6819 det the,content
R4693 T6819 T6816 nsubj content,be
R4694 T6820 T6819 amod minimal,content
R4695 T6821 T6819 compound thymine,content
R4696 T6822 T6819 acl required,content
R4697 T6823 T6822 prep for,required
R4698 T6824 T6823 pobj activation,for
R4699 T6825 T6824 prep of,activation
R470 T960 T961 punct –,14
R4700 T6826 T6827 det the,helicase
R4701 T6827 T6825 pobj helicase,of
R4702 T6828 T6827 compound Mcm,helicase
R4703 T6829 T6816 aux may,be
R4704 T6830 T6816 advmod somewhere,be
R4705 T6831 T6830 prep between,somewhere
R4706 T6832 T6833 nummod 33,%
R4707 T6833 T6831 pobj %,between
R4708 T6834 T6832 cc and,33
R4709 T6835 T6832 conj 50,33
R471 T961 T959 prep 14,10
R4710 T6836 T6816 punct .,be
R4711 T7008 T7009 compound DUE,sequences
R4712 T7009 T7010 nsubj sequences,activate
R4713 T7011 T7009 prep from,sequences
R4714 T7012 T7013 det the,origin
R4715 T7013 T7011 pobj origin,from
R4716 T7014 T7015 compound c,myc
R4717 T7015 T7013 compound myc,origin
R4718 T7016 T7015 punct -,myc
R4719 T7017 T7018 compound Mcm,helicase
R472 T962 T959 punct ",",10
R4720 T7018 T7010 dobj helicase,activate
R4721 T7020 T7021 poss Our,studies
R4722 T7021 T7023 nsubj studies,shown
R4723 T7022 T7021 amod previous,studies
R4724 T7024 T7023 aux have,shown
R4725 T7025 T7026 mark that,serve
R4726 T7026 T7023 ccomp serve,shown
R4727 T7027 T7028 det the,sequences
R4728 T7028 T7026 nsubj sequences,serve
R4729 T7029 T7028 acl containing,sequences
R473 T963 T959 appos 17,10
R4730 T7030 T7031 amod periodic,tracts
R4731 T7031 T7029 dobj tracts,containing
R4732 T7032 T7033 punct (,n
R4733 T7033 T7031 compound n,tracts
R4734 T7034 T7033 nmod dT,n
R4735 T7035 T7033 punct ),n
R4736 T7036 T7031 acl derived,tracts
R4737 T7037 T7036 prep from,derived
R4738 T7038 T7039 det the,origin
R4739 T7039 T7037 pobj origin,from
R474 T964 T965 punct –,18
R4740 T7040 T7039 amod human,origin
R4741 T7041 T7042 compound lamin,B2
R4742 T7042 T7039 compound B2,origin
R4743 T7043 T7044 punct (,content
R4744 T7044 T7039 parataxis content,origin
R4745 T7045 T7046 nummod 48,%
R4746 T7046 T7044 compound %,content
R4747 T7047 T7044 compound thymine,content
R4748 T7048 T7044 punct ),content
R4749 T7049 T7026 aux can,serve
R475 T965 T963 prep 18,17
R4750 T7050 T7026 prep as,serve
R4751 T7051 T7052 det an,activator
R4752 T7052 T7050 pobj activator,as
R4753 T7053 T7052 amod efficient,activator
R4754 T7054 T7052 prep for,activator
R4755 T7055 T7056 det the,helicase
R4756 T7056 T7054 pobj helicase,for
R4757 T7057 T7056 compound Mcm,helicase
R4758 T7058 T7026 prep on,serve
R4759 T7059 T7060 det a,bubble
R476 T966 T959 punct ),10
R4760 T7060 T7058 pobj bubble,on
R4761 T7061 T7023 punct ", ",shown
R4762 T7062 T7023 cc and,shown
R4763 T7063 T7064 nsubj replacement,abolished
R4764 T7064 T7023 conj abolished,shown
R4765 T7065 T7063 prep of,replacement
R4766 T7066 T7065 pobj thymines,of
R4767 T7067 T7063 prep with,replacement
R4768 T7068 T7067 pobj guanines,with
R4769 T7069 T7070 det the,activation
R477 T967 T930 punct .,shown
R4770 T7070 T7064 dobj activation,abolished
R4771 T7071 T7070 compound helicase,activation
R4772 T7072 T7073 punct (,28
R4773 T7073 T7064 parataxis 28,abolished
R4774 T7074 T7073 nummod 13,28
R4775 T7075 T7073 punct ",",28
R4776 T7076 T7073 punct ),28
R4777 T7077 T7064 punct .,abolished
R4778 T7079 T7080 prep In,constructed
R4779 T7081 T7079 pobj order,In
R478 T969 T970 mark While,make
R4780 T7082 T7083 aux to,examine
R4781 T7083 T7081 acl examine,order
R4782 T7084 T7085 amod other,origins
R4783 T7085 T7083 dobj origins,examine
R4784 T7086 T7085 amod natural,origins
R4785 T7087 T7085 compound replication,origins
R4786 T7088 T7085 prep with,origins
R4787 T7089 T7090 amod different,contents
R4788 T7090 T7088 pobj contents,with
R4789 T7091 T7090 compound thymine,contents
R479 T970 T977 advcl make,inhibit
R4790 T7092 T7083 prep for,examine
R4791 T7093 T7094 det the,ability
R4792 T7094 T7092 pobj ability,for
R4793 T7095 T7096 aux to,activate
R4794 T7096 T7094 acl activate,ability
R4795 T7097 T7098 compound Mcm,helicase
R4796 T7098 T7096 dobj helicase,activate
R4797 T7099 T7096 prep on,activate
R4798 T7100 T7101 det a,substrate
R4799 T7101 T7099 pobj substrate,on
R480 T971 T972 nmod Mcm4,proteins
R4800 T7102 T7101 compound bubble,substrate
R4801 T7103 T7080 punct ", ",constructed
R4802 T7104 T7080 nsubj we,constructed
R4803 T7105 T7106 amod new,substrates
R4804 T7106 T7080 dobj substrates,constructed
R4805 T7107 T7106 compound bubble,substrates
R4806 T7108 T7106 punct ", ",substrates
R4807 T7109 T7110 compound Bub82,myc
R4808 T7110 T7106 appos myc,substrates
R4809 T7111 T7110 punct /,myc
R481 T972 T970 nsubj proteins,make
R4810 T7112 T7110 compound c,myc
R4811 T7113 T7110 punct -,myc
R4812 T7114 T7106 punct ", ",substrates
R4813 T7115 T7106 acl containing,substrates
R4814 T7116 T7115 dobj sequences,containing
R4815 T7117 T7116 acl derived,sequences
R4816 T7118 T7117 prep from,derived
R4817 T7119 T7120 det the,region
R4818 T7120 T7118 pobj region,from
R4819 T7121 T7120 nmod DUE,region
R482 T973 T971 punct ", ",Mcm4
R4820 T7122 T7121 punct (,DUE
R4821 T7123 T7124 compound DNA,element
R4822 T7124 T7121 appos element,DUE
R4823 T7125 T7124 compound unwinding,element
R4824 T7126 T7120 punct ),region
R4825 T7127 T7120 prep of,region
R4826 T7128 T7129 det the,origin
R4827 T7129 T7127 pobj origin,of
R4828 T7130 T7129 amod human,origin
R4829 T7131 T7132 compound c,myc
R483 T974 T971 conj Mcm6,Mcm4
R4830 T7132 T7129 compound myc,origin
R4831 T7133 T7132 punct -,myc
R4832 T7134 T7135 dep which,is
R4833 T7135 T7120 relcl is,region
R4834 T7136 T7135 acomp essential,is
R4835 T7137 T7136 prep for,essential
R4836 T7138 T7139 compound c,myc
R4837 T7139 T7141 compound myc,activity
R4838 T7140 T7139 punct -,myc
R4839 T7141 T7137 pobj activity,for
R484 T975 T974 cc and,Mcm6
R4840 T7142 T7141 compound replicator,activity
R4841 T7143 T7144 punct (,30
R4842 T7144 T7135 parataxis 30,is
R4843 T7145 T7144 nummod 29,30
R4844 T7146 T7144 punct ",",30
R4845 T7147 T7144 punct ),30
R4846 T7148 T7080 punct .,constructed
R4847 T7150 T7151 det The,segment
R4848 T7151 T7153 nsubj segment,contains
R4849 T7152 T7151 amod unpaired,segment
R485 T976 T974 conj Mcm7,Mcm6
R4850 T7154 T7151 prep in,segment
R4851 T7155 T7156 compound Bub82,DUE
R4852 T7156 T7154 pobj DUE,in
R4853 T7157 T7156 punct /,DUE
R4854 T7158 T7159 compound c,myc
R4855 T7159 T7156 compound myc,DUE
R4856 T7160 T7159 punct -,myc
R4857 T7161 T7156 punct /,DUE
R4858 T7162 T7163 nummod one,strand
R4859 T7163 T7153 dobj strand,contains
R486 T978 T979 amod distinct,contribution
R4860 T7164 T7163 prep of,strand
R4861 T7165 T7164 pobj DUE,of
R4862 T7166 T7153 cc and,contains
R4863 T7167 T7168 nsubj that,contains
R4864 T7168 T7153 conj contains,contains
R4865 T7169 T7167 prep in,that
R4866 T7170 T7171 compound Bub82,C
R4867 T7171 T7169 pobj C,in
R4868 T7172 T7171 punct /,C
R4869 T7173 T7174 compound c,myc
R487 T979 T970 dobj contribution,make
R4870 T7174 T7171 compound myc,C
R4871 T7175 T7174 punct -,myc
R4872 T7176 T7171 punct /,C
R4873 T7177 T7171 compound DUE,C
R4874 T7178 T7171 punct -,C
R4875 T7179 T7180 det another,strand
R4876 T7180 T7168 dobj strand,contains
R4877 T7181 T7168 punct .,contains
R4878 T7183 T7184 det This,DUE
R4879 T7184 T7185 nsubjpass DUE,believed
R488 T980 T979 prep to,contribution
R4880 T7186 T7185 auxpass is,believed
R4881 T7187 T7188 aux to,coincide
R4882 T7188 T7185 xcomp coincide,believed
R4883 T7189 T7188 prep with,coincide
R4884 T7190 T7191 det the,region
R4885 T7191 T7189 pobj region,with
R4886 T7192 T7193 advmod initially,unwound
R4887 T7193 T7191 amod unwound,region
R4888 T7194 T7191 prep of,region
R4889 T7195 T7196 det the,origin
R489 T981 T982 poss its,activity
R4890 T7196 T7194 pobj origin,of
R4891 T7197 T7198 compound c,myc
R4892 T7198 T7196 compound myc,origin
R4893 T7199 T7198 punct -,myc
R4894 T7200 T7201 punct (,29
R4895 T7201 T7188 parataxis 29,coincide
R4896 T7202 T7201 punct ),29
R4897 T7203 T7185 punct ", ",believed
R4898 T7204 T7185 cc and,believed
R4899 T7205 T7206 poss its,deletion
R490 T982 T980 pobj activity,to
R4900 T7206 T7207 nsubj deletion,reduced
R4901 T7207 T7185 conj reduced,believed
R4902 T7208 T7207 advmod substantially,reduced
R4903 T7209 T7210 det the,activity
R4904 T7210 T7207 dobj activity,reduced
R4905 T7211 T7210 compound replicator,activity
R4906 T7212 T7213 punct (,30
R4907 T7213 T7207 parataxis 30,reduced
R4908 T7214 T7213 punct ),30
R4909 T7215 T7207 punct .,reduced
R491 T983 T982 compound helicase,activity
R4910 T7217 T7218 nsubj Mcm4,unwound
R4911 T7219 T7217 punct /,Mcm4
R4912 T7220 T7217 nummod 6,Mcm4
R4913 T7221 T7217 punct /,Mcm4
R4914 T7222 T7217 nummod 7,Mcm4
R4915 T7223 T7224 compound Bub82,B2
R4916 T7224 T7218 dobj B2,unwound
R4917 T7225 T7224 punct /,B2
R4918 T7226 T7224 compound lamin,B2
R4919 T7227 T7228 punct (,%
R492 T984 T985 punct (,11
R4920 T7228 T7224 parataxis %,B2
R4921 T7229 T7228 nmod thymine,%
R4922 T7230 T7228 nummod 48,%
R4923 T7231 T7228 punct ),%
R4924 T7232 T7224 punct ", ",B2
R4925 T7233 T7234 compound Bub82,DUE
R4926 T7234 T7224 conj DUE,B2
R4927 T7235 T7234 punct /,DUE
R4928 T7236 T7237 compound c,myc
R4929 T7237 T7234 compound myc,DUE
R493 T985 T970 parataxis 11,make
R4930 T7238 T7237 punct -,myc
R4931 T7239 T7234 punct /,DUE
R4932 T7240 T7241 punct (,%
R4933 T7241 T7234 parataxis %,DUE
R4934 T7242 T7241 nummod 37,%
R4935 T7243 T7241 punct ),%
R4936 T7244 T7234 cc and,DUE
R4937 T7245 T7246 compound Bub82,C
R4938 T7246 T7234 conj C,DUE
R4939 T7247 T7246 punct /,C
R494 T986 T987 punct –,13
R4940 T7248 T7249 compound c,myc
R4941 T7249 T7246 compound myc,C
R4942 T7250 T7249 punct -,myc
R4943 T7251 T7246 punct /,C
R4944 T7252 T7246 compound DUE,C
R4945 T7253 T7246 punct -,C
R4946 T7254 T7255 punct (,%
R4947 T7255 T7246 parataxis %,C
R4948 T7256 T7255 nummod 39,%
R4949 T7257 T7255 punct ),%
R495 T987 T985 prep 13,11
R4950 T7258 T7218 prep with,unwound
R4951 T7259 T7260 amod similar,efficiency
R4952 T7260 T7258 pobj efficiency,with
R4953 T7261 T7262 punct (,7A
R4954 T7262 T7260 parataxis 7A,efficiency
R4955 T7263 T7262 compound Figure,7A
R4956 T7264 T7262 punct ),7A
R4957 T7265 T7218 punct ", ",unwound
R4958 T7266 T7218 advcl indicating,unwound
R4959 T7267 T7268 mark that,serve
R496 T988 T985 punct ),11
R4960 T7268 T7266 ccomp serve,indicating
R4961 T7269 T7270 det the,sequences
R4962 T7270 T7268 nsubj sequences,serve
R4963 T7271 T7270 prep from,sequences
R4964 T7272 T7273 det the,origin
R4965 T7273 T7271 pobj origin,from
R4966 T7274 T7275 compound c,myc
R4967 T7275 T7273 compound myc,origin
R4968 T7276 T7275 punct -,myc
R4969 T7277 T7268 advmod also,serve
R497 T989 T977 punct ", ",inhibit
R4970 T7278 T7268 aux can,serve
R4971 T7279 T7268 prep as,serve
R4972 T7280 T7281 amod efficient,activators
R4973 T7281 T7279 pobj activators,as
R4974 T7282 T7281 prep of,activators
R4975 T7283 T7284 det the,helicase
R4976 T7284 T7282 pobj helicase,of
R4977 T7285 T7284 compound Mcm,helicase
R4978 T7286 T7218 punct .,unwound
R4979 T7288 T7289 det This,result
R498 T990 T977 nsubj Mcm2,inhibit
R4980 T7289 T7290 nsubj result,indicates
R4981 T7291 T7290 advmod also,indicates
R4982 T7292 T7293 mark that,activate
R4983 T7293 T7290 ccomp activate,indicates
R4984 T7294 T7293 nsubj sequences,activate
R4985 T7295 T7294 prep with,sequences
R4986 T7296 T7297 nummod 37,%
R4987 T7297 T7298 compound %,content
R4988 T7298 T7295 pobj content,with
R4989 T7299 T7298 compound thymine,content
R499 T991 T990 cc or,Mcm2
R4990 T7300 T7293 aux can,activate
R4991 T7301 T7293 dobj Mcm4,activate
R4992 T7302 T7301 punct /,Mcm4
R4993 T7303 T7301 nummod 6,Mcm4
R4994 T7304 T7301 punct /,Mcm4
R4995 T7305 T7301 nummod 7,Mcm4
R4996 T7306 T7307 aux to,displace
R4997 T7307 T7293 advcl displace,activate
R4998 T7308 T7309 nummod 24,nt
R4999 T7309 T7310 compound nt,duplex
R500 T992 T990 conj Mcm3,Mcm2
R5000 T7310 T7307 dobj duplex,displace
R5001 T7311 T7307 prep on,displace
R5002 T7312 T7313 det both,sides
R5003 T7313 T7311 pobj sides,on
R5004 T7314 T7307 prep in,displace
R5005 T7315 T7316 det a,substrate
R5006 T7316 T7314 pobj substrate,in
R5007 T7317 T7316 compound bubble,substrate
R5008 T7318 T7293 punct ", ",activate
R5009 T7319 T7293 advcl suggesting,activate
R501 T993 T992 punct /,Mcm3
R5010 T7320 T7321 mark that,affect
R5011 T7321 T7319 ccomp affect,suggesting
R5012 T7322 T7323 det the,content
R5013 T7323 T7321 nsubj content,affect
R5014 T7324 T7323 preconj not,content
R5015 T7325 T7324 advmod only,not
R5016 T7326 T7323 compound thymine,content
R5017 T7327 T7323 cc but,content
R5018 T7328 T7327 advmod also,but
R5019 T7329 T7330 det the,context
R502 T994 T992 nummod 5,Mcm3
R5020 T7330 T7323 conj context,content
R5021 T7331 T7330 compound sequence,context
R5022 T7332 T7321 aux may,affect
R5023 T7333 T7334 det the,activation
R5024 T7334 T7321 dobj activation,affect
R5025 T7335 T7336 compound Mcm,helicase
R5026 T7336 T7334 compound helicase,activation
R5027 T7337 T7290 punct .,indicates
R5028 T7339 T7340 prep In,bound
R5029 T7341 T7342 compound gel,shift
R503 T995 T996 det the,activity
R5030 T7342 T7343 compound shift,assays
R5031 T7343 T7339 pobj assays,In
R5032 T7344 T7340 punct ", ",bound
R5033 T7345 T7340 nsubj MCM4,bound
R5034 T7346 T7345 punct /,MCM4
R5035 T7347 T7345 nummod 6,MCM4
R5036 T7348 T7345 punct /,MCM4
R5037 T7349 T7345 nummod 7,MCM4
R5038 T7350 T7340 prep to,bound
R5039 T7351 T7352 predet all,bubbles
R504 T996 T977 dobj activity,inhibit
R5040 T7352 T7350 pobj bubbles,to
R5041 T7353 T7352 det the,bubbles
R5042 T7354 T7352 nummod three,bubbles
R5043 T7355 T7340 punct ", ",bound
R5044 T7356 T7357 mark although,is
R5045 T7357 T7340 advcl is,bound
R5046 T7358 T7359 det the,affinity
R5047 T7359 T7357 nsubj affinity,is
R5048 T7360 T7359 prep to,affinity
R5049 T7361 T7362 compound Bub82,DUE
R505 T997 T996 compound helicase,activity
R5050 T7362 T7360 pobj DUE,to
R5051 T7363 T7362 punct /,DUE
R5052 T7364 T7365 compound c,myc
R5053 T7365 T7362 compound myc,DUE
R5054 T7366 T7365 punct -,myc
R5055 T7367 T7362 punct /,DUE
R5056 T7368 T7360 cc and,to
R5057 T7369 T7360 conj to,to
R5058 T7370 T7371 compound Bub82,C
R5059 T7371 T7369 pobj C,to
R506 T998 T996 prep of,activity
R5060 T7372 T7371 punct /,C
R5061 T7373 T7374 compound c,myc
R5062 T7374 T7371 compound myc,C
R5063 T7375 T7374 punct -,myc
R5064 T7376 T7371 punct /,C
R5065 T7377 T7371 compound DUE,C
R5066 T7378 T7371 punct -,C
R5067 T7379 T7380 advmod slightly,lower
R5068 T7380 T7357 acomp lower,is
R5069 T7381 T7380 prep than,lower
R507 T999 T1000 det the,complex
R5070 T7382 T7381 pobj that,than
R5071 T7383 T7382 prep to,that
R5072 T7384 T7385 nmod Bub,B2
R5073 T7385 T7383 pobj B2,to
R5074 T7386 T7385 punct -,B2
R5075 T7387 T7385 nummod 82,B2
R5076 T7388 T7385 punct /,B2
R5077 T7389 T7385 compound lamin,B2
R5078 T7390 T7391 punct (,7B
R5079 T7391 T7357 parataxis 7B,is
R508 T1000 T998 pobj complex,of
R5080 T7392 T7391 compound Figure,7B
R5081 T7393 T7391 punct ),7B
R5082 T7394 T7340 punct .,bound
R5083 T7396 T7397 advmod Therefore,correlate
R5084 T7398 T7397 punct ", ",correlate
R5085 T7399 T7400 nmod helicase,activities
R5086 T7400 T7397 nsubj activities,correlate
R5087 T7401 T7399 cc and,helicase
R5088 T7402 T7403 compound DNA,binding
R5089 T7403 T7399 conj binding,helicase
R509 T1001 T1000 nmod Mcm4,complex
R5090 T7404 T7397 aux do,correlate
R5091 T7405 T7397 neg not,correlate
R5092 T7406 T7397 advmod necessarily,correlate
R5093 T7407 T7397 prep with,correlate
R5094 T7408 T7409 det each,other
R5095 T7409 T7407 pobj other,with
R5096 T7410 T7397 punct .,correlate
R5097 T7412 T7413 nsubjpass This,indicated
R5098 T7414 T7413 auxpass was,indicated
R5099 T7415 T7413 advmod also,indicated
R510 T1002 T1001 punct /,Mcm4
R5100 T7416 T7413 agent by,indicated
R5101 T7417 T7418 poss our,results
R5102 T7418 T7416 pobj results,by
R5103 T7419 T7418 amod previous,results
R5104 T7420 T7421 mark that,bind
R5105 T7421 T7418 acl bind,results
R5106 T7422 T7423 nmod guanine,stretches
R5107 T7423 T7421 nsubj stretches,bind
R5108 T7424 T7422 cc and,guanine
R5109 T7425 T7422 conj cytosine,guanine
R511 T1003 T1001 nummod 6,Mcm4
R5110 T7426 T7421 aux can,bind
R5111 T7427 T7421 prep to,bind
R5112 T7428 T7427 pobj Mcm,to
R5113 T7429 T7421 cc but,bind
R5114 T7430 T7431 aux can,activate
R5115 T7431 T7421 conj activate,bind
R5116 T7432 T7431 neg not,activate
R5117 T7433 T7434 det the,helicase
R5118 T7434 T7431 dobj helicase,activate
R5119 T7435 T7436 punct (,13
R512 T1004 T1001 punct /,Mcm4
R5120 T7436 T7431 parataxis 13,activate
R5121 T7437 T7436 punct ),13
R5122 T7438 T7413 punct .,indicated
R5124 T7722 T7723 compound DNA,unwinding
R5125 T7723 T7724 nsubjpass unwinding,inhibited
R5126 T7725 T7723 prep by,unwinding
R5127 T7726 T7725 pobj Mcm4,by
R5128 T7727 T7726 punct /,Mcm4
R5129 T7728 T7726 nummod 6,Mcm4
R513 T1005 T1001 nummod 7,Mcm4
R5130 T7729 T7726 punct /,Mcm4
R5131 T7730 T7726 nummod 7,Mcm4
R5132 T7731 T7724 auxpass is,inhibited
R5133 T7732 T7724 agent by,inhibited
R5134 T7733 T7734 det the,sequences
R5135 T7734 T7732 pobj sequences,by
R5136 T7735 T7736 npadvmod GC,rich
R5137 T7736 T7734 amod rich,sequences
R5138 T7737 T7736 punct -,rich
R5139 T7738 T7734 prep on,sequences
R514 T1006 T977 prep by,inhibit
R5140 T7739 T7740 det the,segment
R5141 T7740 T7738 pobj segment,on
R5142 T7741 T7740 compound duplex,segment
R5143 T7743 T7744 poss Our,results
R5144 T7744 T7745 nsubj results,indicate
R5145 T7746 T7747 mark that,required
R5146 T7747 T7745 ccomp required,indicate
R5147 T7748 T7749 npadvmod thymine,rich
R5148 T7749 T7751 amod rich,DNA
R5149 T7750 T7749 punct -,rich
R515 T1007 T1006 pcomp converting,by
R5150 T7751 T7747 nsubjpass DNA,required
R5151 T7752 T7753 amod single,stranded
R5152 T7753 T7751 amod stranded,DNA
R5153 T7754 T7753 punct -,stranded
R5154 T7755 T7747 auxpass is,required
R5155 T7756 T7747 prep for,required
R5156 T7757 T7758 amod initial,loading
R5157 T7758 T7756 pobj loading,for
R5158 T7759 T7758 cc and,loading
R5159 T7760 T7758 conj activation,loading
R516 T1008 T1009 poss its,structure
R5160 T7761 T7758 prep of,loading
R5161 T7762 T7763 det the,helicase
R5162 T7763 T7761 pobj helicase,of
R5163 T7764 T7763 compound Mcm,helicase
R5164 T7765 T7745 punct .,indicate
R5165 T7767 T7768 advmod However,known
R5166 T7769 T7768 punct ", ",known
R5167 T7770 T7768 nsubjpass it,known
R5168 T7771 T7768 auxpass is,known
R5169 T7772 T7768 neg not,known
R517 T1009 T1007 dobj structure,converting
R5170 T7773 T7774 mark whether,required
R5171 T7774 T7768 ccomp required,known
R5172 T7775 T7776 compound thymine,sequences
R5173 T7776 T7774 nsubjpass sequences,required
R5174 T7777 T7774 auxpass are,required
R5175 T7778 T7774 prep for,required
R5176 T7779 T7780 amod processive,unwinding
R5177 T7780 T7778 pobj unwinding,for
R5178 T7781 T7780 prep of,unwinding
R5179 T7782 T7783 compound duplex,DNA
R518 T1010 T1009 amod double,structure
R5180 T7783 T7781 pobj DNA,of
R5181 T7784 T7768 punct .,known
R5182 T7786 T7787 advmod Therefore,examined
R5183 T7788 T7787 punct ", ",examined
R5184 T7789 T7787 nsubj we,examined
R5185 T7790 T7787 aux have,examined
R5186 T7791 T7792 mark whether,affects
R5187 T7792 T7787 ccomp affects,examined
R5188 T7793 T7794 det the,composition
R5189 T7794 T7792 nsubj composition,affects
R519 T1011 T1009 compound trimer,structure
R5190 T7795 T7794 compound nucleotide,composition
R5191 T7796 T7794 prep of,composition
R5192 T7797 T7798 det the,region
R5193 T7798 T7796 pobj region,of
R5194 T7799 T7798 compound duplex,region
R5195 T7800 T7801 poss its,activity
R5196 T7801 T7792 dobj activity,affects
R5197 T7802 T7801 compound unwinding,activity
R5198 T7803 T7787 punct .,examined
R5199 T7805 T7806 aux To,address
R520 T1012 T1007 prep into,converting
R5200 T7806 T7807 advcl address,constructed
R5201 T7808 T7809 det this,issue
R5202 T7809 T7806 dobj issue,address
R5203 T7810 T7807 punct ", ",constructed
R5204 T7811 T7807 nsubj we,constructed
R5205 T7812 T7813 det a,series
R5206 T7813 T7807 dobj series,constructed
R5207 T7814 T7813 prep of,series
R5208 T7815 T7816 npadvmod T,tailed
R5209 T7816 T7818 amod tailed,structures
R521 T1013 T1014 det a,heterotetramer
R5210 T7817 T7816 punct -,tailed
R5211 T7818 T7814 pobj structures,of
R5212 T7819 T7820 compound Y,fork
R5213 T7820 T7818 compound fork,structures
R5214 T7821 T7820 punct -,fork
R5215 T7822 T7818 punct (,structures
R5216 T7823 T7824 compound T,fork
R5217 T7824 T7818 appos fork,structures
R5218 T7825 T7824 punct -,fork
R5219 T7826 T7818 punct ),structures
R522 T1014 T1012 pobj heterotetramer,into
R5220 T7827 T7818 acl containing,structures
R5221 T7828 T7829 amod various,sequences
R5222 T7829 T7827 dobj sequences,containing
R5223 T7830 T7807 prep in,constructed
R5224 T7831 T7832 det the,segment
R5225 T7832 T7830 pobj segment,in
R5226 T7833 T7832 compound duplex,segment
R5227 T7834 T7807 punct .,constructed
R5228 T7836 T7837 nsubj They,carried
R5229 T7838 T7839 amod varied,contents
R523 T1015 T1014 cc or,heterotetramer
R5230 T7839 T7837 dobj contents,carried
R5231 T7840 T7839 prep of,contents
R5232 T7841 T7842 compound cytosine,residues
R5233 T7842 T7840 pobj residues,of
R5234 T7843 T7837 prep on,carried
R5235 T7844 T7845 det the,strand
R5236 T7845 T7843 pobj strand,on
R5237 T7846 T7847 nummod 3,tail
R5238 T7847 T7845 compound tail,strand
R5239 T7848 T7846 punct ′,3
R524 T1016 T1014 conj heteropentamer,heterotetramer
R5240 T7849 T7847 punct -,tail
R5241 T7850 T7837 punct .,carried
R5242 T7852 T7853 prep In,bound
R5243 T7854 T7855 compound gel,shift
R5244 T7855 T7856 compound shift,assays
R5245 T7856 T7852 pobj assays,In
R5246 T7857 T7853 punct ", ",bound
R5247 T7858 T7853 nsubj Mcm4,bound
R5248 T7859 T7858 punct /,Mcm4
R5249 T7860 T7858 nummod 6,Mcm4
R525 T1017 T1007 punct ", ",converting
R5250 T7861 T7858 punct /,Mcm4
R5251 T7862 T7858 nummod 7,Mcm4
R5252 T7863 T7853 prep to,bound
R5253 T7864 T7865 det these,substrates
R5254 T7865 T7863 pobj substrates,to
R5255 T7866 T7867 compound Y,fork
R5256 T7867 T7865 compound fork,substrates
R5257 T7868 T7867 punct -,fork
R5258 T7869 T7853 prep with,bound
R5259 T7870 T7871 amod identical,affinity
R526 T1018 T1007 advmod respectively,converting
R5260 T7871 T7869 pobj affinity,with
R5261 T7872 T7873 punct (,8A
R5262 T7873 T7871 parataxis 8A,affinity
R5263 T7874 T7873 compound Figure,8A
R5264 T7875 T7873 punct ),8A
R5265 T7876 T7853 punct ", ",bound
R5266 T7877 T7853 advcl consistent,bound
R5267 T7878 T7877 prep with,consistent
R5268 T7879 T7880 det the,notion
R5269 T7880 T7878 pobj notion,with
R527 T1019 T1020 punct (,11
R5270 T7881 T7882 mark that,binds
R5271 T7882 T7880 acl binds,notion
R5272 T7883 T7884 det the,Mcm4
R5273 T7884 T7882 nsubj Mcm4,binds
R5274 T7885 T7884 punct /,Mcm4
R5275 T7886 T7884 nummod 6,Mcm4
R5276 T7887 T7884 punct /,Mcm4
R5277 T7888 T7884 nummod 7,Mcm4
R5278 T7889 T7882 prep to,binds
R5279 T7890 T7891 amod single,stranded
R528 T1020 T1007 parataxis 11,converting
R5280 T7891 T7893 amod stranded,tails
R5281 T7892 T7891 punct -,stranded
R5282 T7893 T7889 pobj tails,to
R5283 T7894 T7853 punct .,bound
R5284 T7896 T7897 advmod However,displaced
R5285 T7898 T7897 punct ", ",displaced
R5286 T7899 T7897 prep in,displaced
R5287 T7900 T7901 compound DNA,helicase
R5288 T7901 T7902 compound helicase,assays
R5289 T7902 T7899 pobj assays,in
R529 T1021 T1020 nummod 3,11
R5290 T7903 T7897 punct ", ",displaced
R5291 T7904 T7905 compound T,fork
R5292 T7905 T7897 nsubjpass fork,displaced
R5293 T7906 T7905 punct -,fork
R5294 T7907 T7905 punct /,fork
R5295 T7908 T7909 punct (,G
R5296 T7909 T7905 appos G,fork
R5297 T7910 T7909 nmod C,G
R5298 T7911 T7909 punct :,G
R5299 T7912 T7909 punct ),G
R530 T1022 T1020 punct ",",11
R5300 T7913 T7909 nummod 49,G
R5301 T7914 T7897 auxpass was,displaced
R5302 T7915 T7897 advmod hardly,displaced
R5303 T7916 T7897 agent by,displaced
R5304 T7917 T7916 pobj Mcm4,by
R5305 T7918 T7917 punct /,Mcm4
R5306 T7919 T7917 nummod 6,Mcm4
R5307 T7920 T7917 punct /,Mcm4
R5308 T7921 T7917 nummod 7,Mcm4
R5309 T7922 T7897 punct ", ",displaced
R531 T1023 T1020 punct ),11
R5310 T7923 T7897 cc but,displaced
R5311 T7924 T7925 auxpass was,displaced
R5312 T7925 T7897 conj displaced,displaced
R5313 T7926 T7925 advmod readily,displaced
R5314 T7927 T7925 agent by,displaced
R5315 T7928 T7929 compound SV40,helicase
R5316 T7929 T7927 pobj helicase,by
R5317 T7930 T7931 compound T,antigen
R5318 T7931 T7929 compound antigen,helicase
R5319 T7932 T7931 punct -,antigen
R532 T1024 T977 punct .,inhibit
R5320 T7933 T7929 compound DNA,helicase
R5321 T7934 T7935 punct (,8B
R5322 T7935 T7925 parataxis 8B,displaced
R5323 T7936 T7935 compound Figure,8B
R5324 T7937 T7935 punct ),8B
R5325 T7938 T7897 punct .,displaced
R5326 T7940 T7941 advmod When,inserted
R5327 T7941 T7946 advcl inserted,increased
R5328 T7942 T7941 nsubjpass thymine,inserted
R5329 T7943 T7942 cc or,thymine
R533 T1026 T1027 compound Chromatin,assays
R5330 T7944 T7942 conj adenine,thymine
R5331 T7945 T7941 auxpass is,inserted
R5332 T7947 T7941 prep as,inserted
R5333 T7948 T7949 det every,nucleotide
R5334 T7949 T7947 pobj nucleotide,as
R5335 T7950 T7949 amod third,nucleotide
R5336 T7951 T7952 punct (,repeats
R5337 T7952 T7949 parataxis repeats,nucleotide
R5338 T7953 T7952 prep of,repeats
R5339 T7954 T7953 pobj CCT,of
R534 T1027 T1029 nsubj assays,suggested
R5340 T7955 T7954 cc or,CCT
R5341 T7956 T7954 conj CCA,CCT
R5342 T7957 T7952 punct ),repeats
R5343 T7958 T7946 punct ", ",increased
R5344 T7959 T7960 det the,extent
R5345 T7960 T7946 nsubj extent,increased
R5346 T7961 T7960 prep of,extent
R5347 T7962 T7961 pobj unwinding,of
R5348 T7963 T7964 punct (,GGA
R5349 T7964 T7946 dobj GGA,increased
R535 T1028 T1027 compound immunoprecipitation,assays
R5350 T7965 T7966 nmod T,fork
R5351 T7966 T7964 nmod fork,GGA
R5352 T7967 T7966 punct -,fork
R5353 T7968 T7964 punct /,GGA
R5354 T7969 T7964 punct (,GGA
R5355 T7970 T7964 nmod CCT,GGA
R5356 T7971 T7964 punct :,GGA
R5357 T7972 T7964 punct ),GGA
R5358 T7973 T7964 nummod 16,GGA
R5359 T7974 T7964 cc and,GGA
R536 T1030 T1027 cc and,assays
R5360 T7975 T7976 nmod T,fork
R5361 T7976 T7978 nmod fork,GGT
R5362 T7977 T7976 punct -,fork
R5363 T7978 T7964 conj GGT,GGA
R5364 T7979 T7978 punct /,GGT
R5365 T7980 T7978 punct (,GGT
R5366 T7981 T7978 nmod CCA,GGT
R5367 T7982 T7978 punct :,GGT
R5368 T7983 T7978 punct ),GGT
R5369 T7984 T7978 nummod 16,GGT
R537 T1031 T1032 amod genetic,characterization
R5370 T7985 T7946 punct ),increased
R5371 T7986 T7946 punct .,increased
R5372 T7988 T7989 det The,efficiency
R5373 T7989 T7990 nsubjpass efficiency,correlated
R5374 T7991 T7989 prep of,efficiency
R5375 T7992 T7991 pobj unwinding,of
R5376 T7993 T7990 auxpass is,correlated
R5377 T7994 T7990 advmod roughly,correlated
R5378 T7995 T7990 prep with,correlated
R5379 T7996 T7997 det the,content
R538 T1032 T1027 conj characterization,assays
R5380 T7997 T7995 pobj content,with
R5381 T7998 T7997 prep of,content
R5382 T7999 T8000 compound GC,pairs
R5383 T8000 T7998 pobj pairs,of
R5384 T8001 T8000 prep in,pairs
R5385 T8002 T8003 det the,segment
R5386 T8003 T8001 pobj segment,in
R5387 T8004 T8003 compound duplex,segment
R5388 T8005 T8006 punct [,GGTT
R5389 T8006 T8000 appos GGTT,pairs
R539 T1033 T1027 prep in,assays
R5390 T8007 T8008 nmod T,fork
R5391 T8008 T8006 nmod fork,GGTT
R5392 T8009 T8008 punct -,fork
R5393 T8010 T8006 punct /,GGTT
R5394 T8011 T8006 punct (,GGTT
R5395 T8012 T8006 nmod CCAA,GGTT
R5396 T8013 T8006 punct :,GGTT
R5397 T8014 T8006 punct ),GGTT
R5398 T8015 T8006 nummod 12,GGTT
R5399 T8016 T8006 punct ", ",GGTT
R540 T1034 T1035 compound Saccharomyces,cerevisiae
R5400 T8017 T8018 nmod T,fork
R5401 T8018 T8020 nmod fork,GGAA
R5402 T8019 T8018 punct -,fork
R5403 T8020 T8006 conj GGAA,GGTT
R5404 T8021 T8020 punct /,GGAA
R5405 T8022 T8020 punct (,GGAA
R5406 T8023 T8020 nmod CCTT,GGAA
R5407 T8024 T8020 punct :,GGAA
R5408 T8025 T8020 punct ),GGAA
R5409 T8026 T8020 nummod 12,GGAA
R541 T1035 T1033 pobj cerevisiae,in
R5410 T8027 T8020 punct ", ",GGAA
R5411 T8028 T8029 nmod T,fork
R5412 T8029 T8031 nmod fork,GTTT
R5413 T8030 T8029 punct -,fork
R5414 T8031 T8020 conj GTTT,GGAA
R5415 T8032 T8031 punct /,GTTT
R5416 T8033 T8031 punct (,GTTT
R5417 T8034 T8031 nmod CAAA,GTTT
R5418 T8035 T8031 punct :,GTTT
R5419 T8036 T8031 punct ),GTTT
R542 T1036 T1029 advmod strongly,suggested
R5420 T8037 T8031 nummod 12,GTTT
R5421 T8038 T8031 cc and,GTTT
R5422 T8039 T8040 nmod T,fork
R5423 T8040 T8042 nmod fork,GAAA
R5424 T8041 T8040 punct -,fork
R5425 T8042 T8031 conj GAAA,GTTT
R5426 T8043 T8042 punct /,GAAA
R5427 T8044 T8042 punct (,GAAA
R5428 T8045 T8042 nmod CTTT,GAAA
R5429 T8046 T8042 punct :,GAAA
R543 T1037 T1038 mark that,involved
R5430 T8047 T8042 punct ),GAAA
R5431 T8048 T8042 nummod 12,GAAA
R5432 T8049 T8050 punct ;,8B
R5433 T8050 T7990 parataxis 8B,correlated
R5434 T8051 T8050 compound Figure,8B
R5435 T8052 T8050 punct ],8B
R5436 T8053 T7990 punct .,correlated
R5437 T8055 T8056 nsubj It,appears
R5438 T8057 T8058 mark that,unwound
R5439 T8058 T8056 ccomp unwound,appears
R544 T1038 T1029 ccomp involved,suggested
R5440 T8059 T8060 det the,segment
R5441 T8060 T8058 nsubjpass segment,unwound
R5442 T8061 T8060 compound duplex,segment
R5443 T8062 T8060 acl containing,segment
R5444 T8063 T8064 punct <,50
R5445 T8064 T8065 nummod 50,%
R5446 T8065 T8066 compound %,pairs
R5447 T8066 T8062 dobj pairs,containing
R5448 T8067 T8066 compound GC,pairs
R5449 T8068 T8058 auxpass is,unwound
R545 T1039 T1038 nsubjpass Mcm,involved
R5450 T8069 T8058 advmod efficiently,unwound
R5451 T8070 T8071 punct (,8C
R5452 T8071 T8058 parataxis 8C,unwound
R5453 T8072 T8071 compound Figure,8C
R5454 T8073 T8071 punct ),8C
R5455 T8074 T8056 punct .,appears
R5456 T8076 T8077 nsubj We,examined
R5457 T8078 T8077 advmod next,examined
R5458 T8079 T8080 det the,effect
R5459 T8080 T8077 dobj effect,examined
R546 T1040 T1038 auxpass is,involved
R5460 T8081 T8080 prep of,effect
R5461 T8082 T8083 npadvmod GC,rich
R5462 T8083 T8085 amod rich,segments
R5463 T8084 T8083 punct -,rich
R5464 T8085 T8081 pobj segments,of
R5465 T8086 T8080 prep on,effect
R5466 T8087 T8088 det a,DNA
R5467 T8088 T8086 pobj DNA,on
R5468 T8089 T8088 amod partial,DNA
R5469 T8090 T8088 compound heteroduplex,DNA
R547 T1041 T1042 preconj not,in
R5470 T8091 T8088 prep on,DNA
R5471 T8092 T8093 det a,DNA
R5472 T8093 T8091 pobj DNA,on
R5473 T8094 T8095 amod single,stranded
R5474 T8095 T8093 amod stranded,DNA
R5475 T8096 T8095 punct -,stranded
R5476 T8097 T8093 amod circular,DNA
R5477 T8098 T8077 punct .,examined
R5478 T8100 T8101 nsubj We,constructed
R5479 T8102 T8101 aux have,constructed
R548 T1042 T1038 prep in,involved
R5480 T8103 T8104 nummod two,sets
R5481 T8104 T8101 dobj sets,constructed
R5482 T8105 T8104 prep of,sets
R5483 T8106 T8107 npadvmod 5,tailed
R5484 T8107 T8110 amod tailed,substrates
R5485 T8108 T8106 punct ′,5
R5486 T8109 T8107 punct -,tailed
R5487 T8110 T8105 pobj substrates,of
R5488 T8111 T8112 amod partial,heteroduplex
R5489 T8112 T8110 compound heteroduplex,substrates
R549 T1043 T1041 advmod only,not
R5490 T8113 T8110 compound DNA,substrates
R5491 T8114 T8110 acl containing,substrates
R5492 T8115 T8116 compound duplex,regions
R5493 T8116 T8114 dobj regions,containing
R5494 T8117 T8116 prep of,regions
R5495 T8118 T8119 amod variable,lengths
R5496 T8119 T8117 pobj lengths,of
R5497 T8120 T8104 punct ;,sets
R5498 T8121 T8104 appos one,sets
R5499 T8122 T8121 prep on,one
R550 T1044 T1042 pobj initiation,in
R5500 T8123 T8124 compound M13mp18,vector
R5501 T8124 T8122 pobj vector,on
R5502 T8125 T8121 cc and,one
R5503 T8126 T8127 det the,other
R5504 T8127 T8121 conj other,one
R5505 T8128 T8127 prep on,other
R5506 T8129 T8128 pobj M13mp19,on
R5507 T8130 T8121 acl containing,one
R5508 T8131 T8132 det a,segment
R5509 T8132 T8130 dobj segment,containing
R551 T1045 T1042 cc but,in
R5510 T8133 T8134 nummod 66,nt
R5511 T8134 T8135 npadvmod nt,long
R5512 T8135 T8132 amod long,segment
R5513 T8136 T8137 npadvmod G,rich
R5514 T8137 T8132 amod rich,segment
R5515 T8138 T8137 punct -,rich
R5516 T8139 T8132 advmod downstream,segment
R5517 T8140 T8139 prep of,downstream
R5518 T8141 T8142 det the,oligonucleotide
R5519 T8142 T8140 pobj oligonucleotide,of
R552 T1046 T1045 advmod also,but
R5520 T8143 T8142 amod hybridizing,oligonucleotide
R5521 T8144 T8101 punct .,constructed
R5522 T8146 T8147 nsubj We,found
R5523 T8148 T8149 mark that,displace
R5524 T8149 T8147 ccomp displace,found
R5525 T8150 T8151 det the,helicase
R5526 T8151 T8149 nsubj helicase,displace
R5527 T8152 T8151 nmod Mcm4,helicase
R5528 T8153 T8152 punct /,Mcm4
R5529 T8154 T8152 nummod 6,Mcm4
R553 T1047 T1042 conj in,in
R5530 T8155 T8152 punct /,Mcm4
R5531 T8156 T8152 nummod 7,Mcm4
R5532 T8157 T8149 aux can,displace
R5533 T8158 T8159 compound duplex,DNA
R5534 T8159 T8149 dobj DNA,displace
R5535 T8160 T8161 quantmod up,350
R5536 T8161 T8163 nummod 350,nt
R5537 T8162 T8161 quantmod to,350
R5538 T8163 T8164 npadvmod nt,long
R5539 T8164 T8159 amod long,DNA
R554 T1048 T1049 det the,stage
R5540 T8165 T8149 prep on,displace
R5541 T8166 T8167 poss its,own
R5542 T8167 T8165 pobj own,on
R5543 T8168 T8149 prep on,displace
R5544 T8169 T8170 det the,substrate
R5545 T8170 T8168 pobj substrate,on
R5546 T8171 T8170 amod former,substrate
R5547 T8172 T8173 punct (,8D
R5548 T8173 T8149 parataxis 8D,displace
R5549 T8174 T8173 compound Figure,8D
R555 T1049 T1047 pobj stage,in
R5550 T8175 T8173 punct ", ",8D
R5551 T8176 T8173 amod left,8D
R5552 T8177 T8173 punct ),8D
R5553 T8178 T8147 punct ", ",found
R5554 T8179 T8147 advcl consistent,found
R5555 T8180 T8179 prep with,consistent
R5556 T8181 T8182 amod previous,results
R5557 T8182 T8180 pobj results,with
R5558 T8183 T8184 punct (,13
R5559 T8184 T8182 parataxis 13,results
R556 T1050 T1051 compound DNA,elongation
R5560 T8185 T8184 punct ),13
R5561 T8186 T8147 punct .,found
R5562 T8188 T8189 prep In,inhibited
R5563 T8190 T8188 pobj contrast,In
R5564 T8191 T8189 punct ", ",inhibited
R5565 T8192 T8189 nsubjpass displacement,inhibited
R5566 T8193 T8189 auxpass was,inhibited
R5567 T8194 T8189 prep over,inhibited
R5568 T8195 T8196 det the,segments
R5569 T8196 T8194 pobj segments,over
R557 T1051 T1049 compound elongation,stage
R5570 T8197 T8198 npadvmod GC,rich
R5571 T8198 T8196 amod rich,segments
R5572 T8199 T8198 punct -,rich
R5573 T8200 T8196 prep on,segments
R5574 T8201 T8202 det the,substrate
R5575 T8202 T8200 pobj substrate,on
R5576 T8203 T8202 amod latter,substrate
R5577 T8204 T8205 punct (,panel
R5578 T8205 T8196 parataxis panel,segments
R5579 T8206 T8207 compound Figure,8D
R558 T1052 T1051 compound chain,elongation
R5580 T8207 T8205 dep 8D,panel
R5581 T8208 T8205 punct ", ",panel
R5582 T8209 T8205 amod right,panel
R5583 T8210 T8205 punct ),panel
R5584 T8211 T8189 punct ", ",inhibited
R5585 T8212 T8213 mark although,observed
R5586 T8213 T8189 advcl observed,inhibited
R5587 T8214 T8213 nsubjpass displacement,observed
R5588 T8215 T8216 quantmod up,250
R5589 T8216 T8218 nummod 250,nt
R559 T1053 T1038 prep as,involved
R5590 T8217 T8216 quantmod to,250
R5591 T8218 T8214 appos nt,displacement
R5592 T8219 T8218 prep in,nt
R5593 T8220 T8219 pobj length,in
R5594 T8221 T8218 punct ", ",nt
R5595 T8222 T8223 dep albeit,at
R5596 T8223 T8218 prep at,nt
R5597 T8224 T8225 det a,level
R5598 T8225 T8223 pobj level,at
R5599 T8226 T8225 amod reduced,level
R560 T1054 T1055 det a,helicase
R5600 T8227 T8213 punct ", ",observed
R5601 T8228 T8213 auxpass was,observed
R5602 T8229 T8213 prep at,observed
R5603 T8230 T8231 det a,concentration
R5604 T8231 T8229 pobj concentration,at
R5605 T8232 T8231 amod high,concentration
R5606 T8233 T8231 prep of,concentration
R5607 T8234 T8235 nmod Mcm4,complex
R5608 T8235 T8233 pobj complex,of
R5609 T8236 T8234 punct /,Mcm4
R561 T1055 T1053 pobj helicase,as
R5610 T8237 T8234 nummod 6,Mcm4
R5611 T8238 T8234 punct /,Mcm4
R5612 T8239 T8234 nummod 7,Mcm4
R5613 T8240 T8189 punct .,inhibited
R5614 T8242 T8243 det This,result
R5615 T8243 T8244 nsubj result,is
R5616 T8245 T8244 acomp consistent,is
R5617 T8246 T8245 prep with,consistent
R5618 T8247 T8246 pobj that,with
R5619 T8248 T8247 prep on,that
R562 T1056 T1055 amod replicative,helicase
R5620 T8249 T8250 compound Y,fork
R5621 T8250 T8248 pobj fork,on
R5622 T8251 T8250 punct -,fork
R5623 T8252 T8244 cc and,is
R5624 T8253 T8244 conj indicates,is
R5625 T8254 T8255 mark that,is
R5626 T8255 T8253 ccomp is,indicates
R5627 T8256 T8257 npadvmod GC,rich
R5628 T8257 T8259 amod rich,segment
R5629 T8258 T8257 punct -,rich
R563 T1057 T1058 punct (,20
R5630 T8259 T8255 nsubj segment,is
R5631 T8260 T8259 compound duplex,segment
R5632 T8261 T8255 acomp inhibitory,is
R5633 T8262 T8261 prep for,inhibitory
R5634 T8263 T8262 pobj unwinding,for
R5635 T8264 T8263 prep by,unwinding
R5636 T8265 T8266 det the,helicase
R5637 T8266 T8264 pobj helicase,by
R5638 T8267 T8266 compound Mcm,helicase
R5639 T8268 T8244 punct .,is
R564 T1058 T1029 parataxis 20,suggested
R5640 T8270 T8271 det These,results
R5641 T8271 T8272 nsubj results,indicate
R5642 T8273 T8272 aux may,indicate
R5643 T8274 T8275 mark that,facilitate
R5644 T8275 T8272 ccomp facilitate,indicate
R5645 T8276 T8277 det the,presence
R5646 T8277 T8275 nsubj presence,facilitate
R5647 T8278 T8277 prep of,presence
R5648 T8279 T8280 compound thymine,adenine
R5649 T8280 T8282 compound adenine,pairs
R565 T1059 T1058 nummod 19,20
R5650 T8281 T8280 punct –,adenine
R5651 T8282 T8278 pobj pairs,of
R5652 T8283 T8282 prep with,pairs
R5653 T8284 T8285 det a,frequency
R5654 T8285 T8283 pobj frequency,with
R5655 T8286 T8285 amod certain,frequency
R5656 T8287 T8277 prep in,presence
R5657 T8288 T8289 det the,region
R5658 T8289 T8287 pobj region,in
R5659 T8290 T8289 compound duplex,region
R566 T1060 T1058 punct ",",20
R5660 T8291 T8275 aux may,facilitate
R5661 T8292 T8293 amod continuous,unwinding
R5662 T8293 T8275 dobj unwinding,facilitate
R5663 T8294 T8293 prep by,unwinding
R5664 T8295 T8294 pobj Mcm4,by
R5665 T8296 T8295 punct /,Mcm4
R5666 T8297 T8295 nummod 6,Mcm4
R5667 T8298 T8295 punct /,Mcm4
R5668 T8299 T8295 nummod 7,Mcm4
R5669 T8300 T8272 punct .,indicate
R567 T1061 T1058 punct ),20
R5670 T8302 T8303 advmod Alternatively,is
R5671 T8304 T8303 punct ", ",is
R5672 T8305 T8303 nsubj Mcm4,is
R5673 T8306 T8305 punct /,Mcm4
R5674 T8307 T8305 nummod 6,Mcm4
R5675 T8308 T8305 punct /,Mcm4
R5676 T8309 T8305 nummod 7,Mcm4
R5677 T8310 T8303 neg not,is
R5678 T8311 T8303 acomp efficient,is
R5679 T8312 T8311 advmod enough,efficient
R568 T1062 T1029 punct .,suggested
R5680 T8313 T8314 aux to,displace
R5681 T8314 T8311 xcomp displace,efficient
R5682 T8315 T8316 advmod thermodynamically,stable
R5683 T8316 T8317 amod stable,segment
R5684 T8317 T8314 dobj segment,displace
R5685 T8318 T8319 npadvmod GC,paired
R5686 T8319 T8317 amod paired,segment
R5687 T8320 T8319 punct -,paired
R5688 T8321 T8319 npadvmod base,paired
R5689 T8322 T8319 punct -,paired
R569 T1064 T1065 advcl Consistent,stimulated
R5690 T8323 T8303 punct .,is
R5691 T8325 T8326 prep In,designed
R5692 T8327 T8325 pobj order,In
R5693 T8328 T8329 aux to,distinguish
R5694 T8329 T8327 acl distinguish,order
R5695 T8330 T8331 det these,possibilities
R5696 T8331 T8329 dobj possibilities,distinguish
R5697 T8332 T8331 nummod two,possibilities
R5698 T8333 T8326 punct ", ",designed
R5699 T8334 T8326 nsubj we,designed
R570 T1066 T1064 prep with,Consistent
R5700 T8335 T8336 det a,substrate
R5701 T8336 T8326 dobj substrate,designed
R5702 T8337 T8336 amod new,substrate
R5703 T8338 T8339 compound T,fork
R5704 T8339 T8336 compound fork,substrate
R5705 T8340 T8339 punct -,fork
R5706 T8341 T8336 acl containing,substrate
R5707 T8342 T8343 nmod inosine,residue
R5708 T8343 T8341 dobj residue,containing
R5709 T8344 T8342 punct (,inosine
R571 T1067 T1068 det this,notion
R5710 T8345 T8342 appos I,inosine
R5711 T8346 T8343 punct ),residue
R5712 T8347 T8348 advmod instead,of
R5713 T8348 T8343 prep of,residue
R5714 T8349 T8350 compound guanosine,residue
R5715 T8350 T8348 pobj residue,of
R5716 T8351 T8326 punct .,designed
R5717 T8353 T8354 det The,stability
R5718 T8354 T8356 nsubj stability,is
R5719 T8355 T8354 amod thermal,stability
R572 T1068 T1066 pobj notion,with
R5720 T8357 T8354 prep of,stability
R5721 T8358 T8359 det the,pair
R5722 T8359 T8357 pobj pair,of
R5723 T8360 T8361 nmod I,C
R5724 T8361 T8359 compound C,pair
R5725 T8362 T8361 punct :,C
R5726 T8363 T8359 compound base,pair
R5727 T8364 T8356 acomp lower,is
R5728 T8365 T8364 prep than,lower
R5729 T8366 T8365 pobj that,than
R573 T1069 T1065 punct ", ",stimulated
R5730 T8367 T8366 prep of,that
R5731 T8368 T8369 det the,pair
R5732 T8369 T8367 pobj pair,of
R5733 T8370 T8371 nmod G,C
R5734 T8371 T8369 compound C,pair
R5735 T8372 T8371 punct :,C
R5736 T8373 T8369 compound base,pair
R5737 T8374 T8356 prep in,is
R5738 T8375 T8376 compound duplex,DNA
R5739 T8376 T8374 pobj DNA,in
R574 T1070 T1071 det the,processivity
R5740 T8377 T8356 prep due,is
R5741 T8378 T8377 pcomp to,due
R5742 T8379 T8377 pobj loss,due
R5743 T8380 T8379 prep of,loss
R5744 T8381 T8382 nummod one,bond
R5745 T8382 T8380 pobj bond,of
R5746 T8383 T8382 compound hydrogen,bond
R5747 T8384 T8356 cc and,is
R5748 T8385 T8356 conj is,is
R5749 T8386 T8387 advmod even,lower
R575 T1071 T1065 nsubjpass processivity,stimulated
R5750 T8387 T8385 acomp lower,is
R5751 T8388 T8387 prep than,lower
R5752 T8389 T8390 nmod A,T
R5753 T8390 T8392 compound T,pair
R5754 T8391 T8390 punct :,T
R5755 T8392 T8388 pobj pair,than
R5756 T8393 T8392 compound base,pair
R5757 T8394 T8395 punct (,31
R5758 T8395 T8392 parataxis 31,pair
R5759 T8396 T8395 punct ),31
R576 T1072 T1071 prep of,processivity
R5760 T8397 T8356 punct .,is
R5761 T8399 T8400 nsubj We,constructed
R5762 T8401 T8402 det the,substrates
R5763 T8402 T8400 dobj substrates,constructed
R5764 T8403 T8404 npadvmod T,tailed
R5765 T8404 T8402 amod tailed,substrates
R5766 T8405 T8404 punct -,tailed
R5767 T8406 T8402 compound fork,substrates
R5768 T8407 T8402 acl containing,substrates
R5769 T8408 T8409 det the,segment
R577 T1073 T1074 det the,complexes
R5770 T8409 T8407 dobj segment,containing
R5771 T8410 T8411 punct (,CGG
R5772 T8411 T8409 nmod CGG,segment
R5773 T8412 T8411 nmod GCC,CGG
R5774 T8413 T8411 punct :,CGG
R5775 T8414 T8411 punct ),CGG
R5776 T8415 T8411 nummod 10,CGG
R5777 T8416 T8411 punct ", ",CGG
R5778 T8417 T8418 punct (,CIG
R5779 T8418 T8411 conj CIG,CGG
R578 T1074 T1072 pobj complexes,of
R5780 T8419 T8418 nmod GCC,CIG
R5781 T8420 T8418 punct :,CIG
R5782 T8421 T8418 punct ),CIG
R5783 T8422 T8418 nummod 10,CIG
R5784 T8423 T8418 cc or,CIG
R5785 T8424 T8425 punct (,CTT
R5786 T8425 T8418 conj CTT,CIG
R5787 T8426 T8425 nmod GAA,CTT
R5788 T8427 T8425 punct :,CTT
R5789 T8428 T8425 punct ),CTT
R579 T1075 T1076 nmod Schizosaccharomyces,pombe
R5790 T8429 T8425 nummod 10,CTT
R5791 T8430 T8409 compound duplex,segment
R5792 T8431 T8409 compound DNA,segment
R5793 T8432 T8433 punct (,8E
R5794 T8433 T8407 parataxis 8E,containing
R5795 T8434 T8433 compound Figure,8E
R5796 T8435 T8433 punct ),8E
R5797 T8436 T8400 punct .,constructed
R5798 T8438 T8439 nsubj Mcm4,displaced
R5799 T8440 T8438 punct /,Mcm4
R580 T1076 T1074 nmod pombe,complexes
R5800 T8441 T8438 nummod 6,Mcm4
R5801 T8442 T8438 punct /,Mcm4
R5802 T8443 T8438 nummod 7,Mcm4
R5803 T8444 T8439 advmod hardly,displaced
R5804 T8445 T8446 det the,duplex
R5805 T8446 T8439 dobj duplex,displaced
R5806 T8447 T8448 nummod 31,nt
R5807 T8448 T8449 npadvmod nt,long
R5808 T8449 T8446 amod long,duplex
R5809 T8450 T8446 prep of,duplex
R581 T1077 T1076 cc and,pombe
R5810 T8451 T8452 nmod GCC,CGG
R5811 T8452 T8454 compound CGG,repeats
R5812 T8453 T8452 punct :,CGG
R5813 T8454 T8450 pobj repeats,of
R5814 T8455 T8439 punct ", ",displaced
R5815 T8456 T8457 mark whereas,displaced
R5816 T8457 T8439 advcl displaced,displaced
R5817 T8458 T8459 det the,CTT
R5818 T8459 T8457 nsubjpass CTT,displaced
R5819 T8460 T8461 nmod T,fork
R582 T1078 T1079 compound mouse,Mcm4
R5820 T8461 T8459 nmod fork,CTT
R5821 T8462 T8461 punct -,fork
R5822 T8463 T8459 punct /,CTT
R5823 T8464 T8459 punct (,CTT
R5824 T8465 T8459 nmod GAA,CTT
R5825 T8466 T8459 punct :,CTT
R5826 T8467 T8459 punct ),CTT
R5827 T8468 T8459 nummod 10,CTT
R5828 T8469 T8457 auxpass was,displaced
R5829 T8470 T8457 advmod efficiently,displaced
R583 T1079 T1076 conj Mcm4,pombe
R5830 T8471 T8457 punct ", ",displaced
R5831 T8472 T8473 mark as,described
R5832 T8473 T8457 advcl described,displaced
R5833 T8474 T8473 advmod above,described
R5834 T8475 T8439 punct .,displaced
R5835 T8477 T8478 nmod T,fork
R5836 T8478 T8480 nmod fork,CIG
R5837 T8479 T8478 punct -,fork
R5838 T8480 T8485 nsubjpass CIG,displaced
R5839 T8481 T8480 punct /,CIG
R584 T1080 T1079 punct /,Mcm4
R5840 T8482 T8480 punct (,CIG
R5841 T8483 T8480 nmod GCC,CIG
R5842 T8484 T8480 punct :,CIG
R5843 T8486 T8480 punct ),CIG
R5844 T8487 T8480 nummod 10,CIG
R5845 T8488 T8485 auxpass was,displaced
R5846 T8489 T8485 prep with,displaced
R5847 T8490 T8489 pobj efficiency,with
R5848 T8491 T8492 advmod much,greater
R5849 T8492 T8490 amod greater,efficiency
R585 T1081 T1079 nummod 6,Mcm4
R5850 T8493 T8492 prep than,greater
R5851 T8494 T8493 pobj that,than
R5852 T8495 T8494 prep of,that
R5853 T8496 T8497 nmod T,fork
R5854 T8497 T8499 nmod fork,CGG
R5855 T8498 T8497 punct -,fork
R5856 T8499 T8495 pobj CGG,of
R5857 T8500 T8497 punct /,fork
R5858 T8501 T8499 punct (,CGG
R5859 T8502 T8499 nmod GCC,CGG
R586 T1082 T1079 punct /,Mcm4
R5860 T8503 T8499 punct :,CGG
R5861 T8504 T8499 punct ),CGG
R5862 T8505 T8499 nummod 10,CGG
R5863 T8506 T8485 punct ", ",displaced
R5864 T8507 T8485 advcl indicating,displaced
R5865 T8508 T8509 mark that,is
R5866 T8509 T8507 ccomp is,indicating
R5867 T8510 T8511 det the,thermostability
R5868 T8511 T8509 nsubj thermostability,is
R5869 T8512 T8511 prep of,thermostability
R587 T1083 T1079 nummod 7,Mcm4
R5870 T8513 T8514 det the,segment
R5871 T8514 T8512 pobj segment,of
R5872 T8515 T8514 compound duplex,segment
R5873 T8516 T8511 punct ", ",thermostability
R5874 T8517 T8511 cc not,thermostability
R5875 T8518 T8519 det the,lack
R5876 T8519 T8511 conj lack,thermostability
R5877 T8520 T8519 prep of,lack
R5878 T8521 T8522 compound AT,pair
R5879 T8522 T8520 pobj pair,of
R588 T1084 T1065 auxpass is,stimulated
R5880 T8523 T8522 compound base,pair
R5881 T8524 T8509 punct ", ",is
R5882 T8525 T8509 acomp responsible,is
R5883 T8526 T8525 prep for,responsible
R5884 T8527 T8526 pobj inability,for
R5885 T8528 T8527 prep of,inability
R5886 T8529 T8528 pobj Mcm4,of
R5887 T8530 T8529 punct /,Mcm4
R5888 T8531 T8529 nummod 6,Mcm4
R5889 T8532 T8529 punct /,Mcm4
R589 T1085 T1065 advmod significantly,stimulated
R5890 T8533 T8529 nummod 7,Mcm4
R5891 T8534 T8535 aux to,displace
R5892 T8535 T8527 acl displace,inability
R5893 T8536 T8537 det the,duplex
R5894 T8537 T8535 dobj duplex,displace
R5895 T8538 T8485 punct .,displaced
R5896 T8790 T8791 compound DNA,helicase
R5897 T8791 T8792 nsubj helicase,is
R5898 T8793 T8794 det a,factor
R5899 T8794 T8792 attr factor,is
R590 T1086 T1065 prep on,stimulated
R5900 T8795 T8794 amod central,factor
R5901 T8796 T8794 prep for,factor
R5902 T8797 T8796 pobj operation,for
R5903 T8798 T8797 prep of,operation
R5904 T8799 T8800 compound replication,forks
R5905 T8800 T8798 pobj forks,of
R5906 T8801 T8792 punct .,is
R5907 T8803 T8804 nsubj It,plays
R5908 T8805 T8804 advmod also,plays
R5909 T8806 T8807 det a,role
R591 T1087 T1088 amod forked,DNA
R5910 T8807 T8804 dobj role,plays
R5911 T8808 T8807 amod crucial,role
R5912 T8809 T8804 prep in,plays
R5913 T8810 T8811 det the,step
R5914 T8811 T8809 pobj step,in
R5915 T8812 T8811 compound initiation,step
R5916 T8813 T8804 prep at,plays
R5917 T8814 T8815 det the,origins
R5918 T8815 T8813 pobj origins,at
R5919 T8816 T8815 compound replication,origins
R592 T1088 T1089 compound DNA,structures
R5920 T8817 T8804 punct .,plays
R5921 T8819 T8820 advmod Therefore,is
R5922 T8821 T8820 punct ", ",is
R5923 T8822 T8823 det the,elucidation
R5924 T8823 T8820 nsubj elucidation,is
R5925 T8824 T8823 prep of,elucidation
R5926 T8825 T8826 advmod how,interact
R5927 T8826 T8824 pobj interact,of
R5928 T8827 T8828 det the,helicases
R5929 T8828 T8826 nsubj helicases,interact
R593 T1089 T1086 pobj structures,on
R5930 T8829 T8828 amod replicative,helicases
R5931 T8830 T8826 prep with,interact
R5932 T8831 T8832 compound DNA,substrate
R5933 T8832 T8830 pobj substrate,with
R5934 T8833 T8826 cc and,interact
R5935 T8834 T8835 advmod how,activated
R5936 T8835 T8826 conj activated,interact
R5937 T8836 T8835 nsubjpass they,activated
R5938 T8837 T8835 auxpass are,activated
R5939 T8838 T8820 acomp crucial,is
R594 T1090 T1065 cc and,stimulated
R5940 T8839 T8838 prep for,crucial
R5941 T8840 T8839 pcomp understanding,for
R5942 T8841 T8842 det the,basis
R5943 T8842 T8840 dobj basis,understanding
R5944 T8843 T8842 amod molecular,basis
R5945 T8844 T8842 prep of,basis
R5946 T8845 T8844 pobj initiation,of
R5947 T8846 T8845 prep of,initiation
R5948 T8847 T8848 compound DNA,replication
R5949 T8848 T8846 pobj replication,of
R595 T1091 T1092 nsubj it,unwind
R5950 T8849 T8820 punct .,is
R5951 T8851 T8852 nsubjpass It,believed
R5952 T8853 T8852 auxpass is,believed
R5953 T8854 T8852 advmod now,believed
R5954 T8855 T8856 mark that,is
R5955 T8856 T8852 ccomp is,believed
R5956 T8857 T8856 nsubj Mcm,is
R5957 T8858 T8859 det a,component
R5958 T8859 T8856 attr component,is
R5959 T8860 T8859 amod major,component
R596 T1092 T1065 conj unwind,stimulated
R5960 T8861 T8859 prep of,component
R5961 T8862 T8863 det the,helicase
R5962 T8863 T8861 pobj helicase,of
R5963 T8864 T8863 amod eukaryotic,helicase
R5964 T8865 T8863 amod replicative,helicase
R5965 T8866 T8852 punct .,believed
R5966 T8868 T8869 prep In,conducted
R5967 T8870 T8871 det this,report
R5968 T8871 T8868 pobj report,In
R5969 T8872 T8869 punct ", ",conducted
R597 T1093 T1092 aux can,unwind
R5970 T8873 T8869 nsubj we,conducted
R5971 T8874 T8869 aux have,conducted
R5972 T8875 T8876 amod detailed,analyses
R5973 T8876 T8869 dobj analyses,conducted
R5974 T8877 T8876 prep on,analyses
R5975 T8878 T8879 nmod binding,actions
R5976 T8879 T8877 pobj actions,on
R5977 T8880 T8878 cc and,binding
R5978 T8881 T8878 conj helicase,binding
R5979 T8882 T8879 prep of,actions
R598 T1094 T1095 compound duplex,DNA
R5980 T8883 T8884 compound mouse,Mcm4
R5981 T8884 T8882 pobj Mcm4,of
R5982 T8885 T8884 punct /,Mcm4
R5983 T8886 T8884 nummod 6,Mcm4
R5984 T8887 T8884 punct /,Mcm4
R5985 T8888 T8884 nummod 7,Mcm4
R5986 T8889 T8869 advcl using,conducted
R5987 T8890 T8891 amod various,substrates
R5988 T8891 T8889 dobj substrates,using
R5989 T8892 T8891 prep including,substrates
R599 T1095 T1092 dobj DNA,unwind
R5990 T8893 T8894 nmod fork,structures
R5991 T8894 T8892 pobj structures,including
R5992 T8895 T8893 cc and,fork
R5993 T8896 T8893 conj bubble,fork
R5994 T8897 T8869 punct .,conducted
R5997 T9089 T9088 prep of,Modes
R5998 T9090 T9089 pobj binding,of
R5999 T9091 T9090 cc and,binding
R600 T1096 T1095 prep of,DNA
R6000 T9092 T9090 conj activation,binding
R6001 T9093 T9090 prep of,binding
R6002 T9094 T9095 nmod Mcm4,helicase
R6003 T9095 T9093 pobj helicase,of
R6004 T9096 T9094 punct /,Mcm4
R6005 T9097 T9094 nummod 6,Mcm4
R6006 T9098 T9094 punct /,Mcm4
R6007 T9099 T9094 nummod 7,Mcm4
R6008 T9100 T9090 prep on,binding
R6009 T9101 T9102 det a,structure
R601 T1097 T1098 quantmod 400,500
R6010 T9102 T9100 pobj structure,on
R6011 T9103 T9102 compound bubble,structure
R6012 T9105 T9106 nsubjpass Initiation,associated
R6013 T9107 T9105 prep of,Initiation
R6014 T9108 T9109 compound DNA,replication
R6015 T9109 T9107 pobj replication,of
R6016 T9110 T9106 auxpass is,associated
R6017 T9111 T9106 prep with,associated
R6018 T9112 T9113 amod localized,melting
R6019 T9113 T9111 pobj melting,with
R602 T1098 T1100 nummod 500,bp
R6020 T9114 T9113 prep of,melting
R6021 T9115 T9116 compound duplex,DNA
R6022 T9116 T9114 pobj DNA,of
R6023 T9117 T9113 amod near,melting
R6024 T9118 T9119 compound replication,origins
R6025 T9119 T9117 npadvmod origins,near
R6026 T9120 T9106 punct .,associated
R6027 T9122 T9123 nsubjpass Helicases,loaded
R6028 T9124 T9123 auxpass are,loaded
R6029 T9125 T9123 prep onto,loaded
R603 T1099 T1098 punct –,500
R6030 T9126 T9127 compound replication,forks
R6031 T9127 T9125 pobj forks,onto
R6032 T9128 T9123 prep through,loaded
R6033 T9129 T9130 det the,region
R6034 T9130 T9128 pobj region,through
R6035 T9131 T9130 amod melted,region
R6036 T9132 T9130 punct ", ",region
R6037 T9133 T9130 acl induced,region
R6038 T9134 T9133 prep by,induced
R6039 T9135 T9136 compound initiator,binding
R604 T1100 T1096 pobj bp,of
R6040 T9136 T9134 pobj binding,by
R6041 T9137 T9123 punct ", ",loaded
R6042 T9138 T9123 prep in,loaded
R6043 T9139 T9138 pobj bacteria,in
R6044 T9140 T9141 punct (,32
R6045 T9141 T9123 parataxis 32,loaded
R6046 T9142 T9141 punct ),32
R6047 T9143 T9123 punct .,loaded
R6048 T9145 T9146 nsubj We,reported
R6049 T9147 T9146 advmod previously,reported
R605 T1101 T1102 punct (,14
R6050 T9148 T9149 mark that,loaded
R6051 T9149 T9146 ccomp loaded,reported
R6052 T9150 T9149 nsubjpass Mcm4,loaded
R6053 T9151 T9150 punct /,Mcm4
R6054 T9152 T9150 nummod 6,Mcm4
R6055 T9153 T9150 punct /,Mcm4
R6056 T9154 T9150 nummod 7,Mcm4
R6057 T9155 T9149 aux can,loaded
R6058 T9156 T9149 auxpass be,loaded
R6059 T9157 T9149 prep onto,loaded
R606 T1102 T1092 parataxis 14,unwind
R6060 T9158 T9159 det a,structure
R6061 T9159 T9157 pobj structure,onto
R6062 T9160 T9161 npadvmod bubble,like
R6063 T9161 T9159 amod like,structure
R6064 T9162 T9161 punct -,like
R6065 T9163 T9146 cc and,reported
R6066 T9164 T9165 aux can,serve
R6067 T9165 T9146 conj serve,reported
R6068 T9166 T9165 prep as,serve
R6069 T9167 T9168 det a,helicase
R607 T1103 T1102 nummod 13,14
R6070 T9168 T9166 pobj helicase,as
R6071 T9169 T9168 compound DNA,helicase
R6072 T9170 T9165 prep at,serve
R6073 T9171 T9172 det the,forks
R6074 T9172 T9170 pobj forks,at
R6075 T9173 T9174 punct (,13
R6076 T9174 T9165 parataxis 13,serve
R6077 T9175 T9174 punct ),13
R6078 T9176 T9146 punct .,reported
R6079 T9178 T9179 det The,ability
R608 T1104 T1102 punct ",",14
R6080 T9179 T9180 nsubj ability,indicates
R6081 T9181 T9179 prep of,ability
R6082 T9182 T9181 pobj Mcm4,of
R6083 T9183 T9182 punct /,Mcm4
R6084 T9184 T9182 nummod 6,Mcm4
R6085 T9185 T9182 punct /,Mcm4
R6086 T9186 T9182 nummod 7,Mcm4
R6087 T9187 T9188 aux to,unwind
R6088 T9188 T9179 acl unwind,ability
R6089 T9189 T9190 det the,substrate
R609 T1105 T1102 punct ),14
R6090 T9190 T9188 dobj substrate,unwind
R6091 T9191 T9190 compound bubble,substrate
R6092 T9192 T9190 cc but,substrate
R6093 T9193 T9192 neg not,but
R6094 T9194 T9195 det the,DNA
R6095 T9195 T9190 conj DNA,substrate
R6096 T9196 T9195 amod conventional,DNA
R6097 T9197 T9195 compound duplex,DNA
R6098 T9198 T9199 punct (,2
R6099 T9199 T9188 parataxis 2,unwind
R610 T1106 T1092 punct .,unwind
R6100 T9200 T9199 nmod Figures,2
R6101 T9201 T9199 cc and,2
R6102 T9202 T9199 conj 13,2
R6103 T9203 T9199 punct ),2
R6104 T9204 T9205 mark that,loaded
R6105 T9205 T9180 ccomp loaded,indicates
R6106 T9206 T9205 nsubjpass Mcm,loaded
R6107 T9207 T9205 aux can,loaded
R6108 T9208 T9205 auxpass be,loaded
R6109 T9209 T9205 prep through,loaded
R611 T1108 T1109 nsubj Mcm4,binds
R6110 T9210 T9211 det the,segment
R6111 T9211 T9209 pobj segment,through
R6112 T9212 T9213 amod single,stranded
R6113 T9213 T9211 amod stranded,segment
R6114 T9214 T9213 punct -,stranded
R6115 T9215 T9211 prep of,segment
R6116 T9216 T9217 det the,bubble
R6117 T9217 T9215 pobj bubble,of
R6118 T9218 T9180 punct .,indicates
R6119 T9220 T9221 advmod Furthermore,displays
R612 T1110 T1108 punct /,Mcm4
R6120 T9222 T9221 punct ", ",displays
R6121 T9223 T9224 det the,complex
R6122 T9224 T9221 nsubj complex,displays
R6123 T9225 T9224 nmod Mcm4,complex
R6124 T9226 T9225 punct /,Mcm4
R6125 T9227 T9225 nummod 6,Mcm4
R6126 T9228 T9225 punct /,Mcm4
R6127 T9229 T9225 nummod 7,Mcm4
R6128 T9230 T9231 amod marked,preference
R6129 T9231 T9221 dobj preference,displays
R613 T1111 T1108 nummod 6,Mcm4
R6130 T9232 T9231 prep for,preference
R6131 T9233 T9234 npadvmod thymine,rich
R6132 T9234 T9236 amod rich,sequences
R6133 T9235 T9234 punct -,rich
R6134 T9236 T9232 pobj sequences,for
R6135 T9237 T9221 prep within,displays
R6136 T9238 T9239 det the,segment
R6137 T9239 T9237 pobj segment,within
R6138 T9240 T9241 amod single,stranded
R6139 T9241 T9239 amod stranded,segment
R614 T1112 T1108 punct /,Mcm4
R6140 T9242 T9241 punct -,stranded
R6141 T9243 T9239 prep for,segment
R6142 T9244 T9245 compound helicase,activation
R6143 T9245 T9243 pobj activation,for
R6144 T9246 T9247 punct (,13
R6145 T9247 T9221 parataxis 13,displays
R6146 T9248 T9247 punct ),13
R6147 T9249 T9221 punct .,displays
R6148 T9251 T9252 det The,results
R6149 T9252 T9253 nsubj results,indicate
R615 T1113 T1108 nummod 7,Mcm4
R6150 T9254 T9252 prep in,results
R6151 T9255 T9256 det this,report
R6152 T9256 T9254 pobj report,in
R6153 T9257 T9258 mark that,depend
R6154 T9258 T9253 ccomp depend,indicate
R6155 T9259 T9260 compound helicase,action
R6156 T9260 T9258 nsubj action,depend
R6157 T9261 T9260 prep of,action
R6158 T9262 T9261 pobj Mcm4,of
R6159 T9263 T9262 punct /,Mcm4
R616 T1114 T1109 prep to,binds
R6160 T9264 T9262 nummod 6,Mcm4
R6161 T9265 T9262 punct /,Mcm4
R6162 T9266 T9262 nummod 7,Mcm4
R6163 T9267 T9260 prep on,action
R6164 T9268 T9269 amod synthetic,bubbles
R6165 T9269 T9267 pobj bubbles,on
R6166 T9270 T9258 aux may,depend
R6167 T9271 T9258 prep on,depend
R6168 T9272 T9273 det the,presence
R6169 T9273 T9271 pobj presence,on
R617 T1115 T1116 nmod fork,structures
R6170 T9274 T9273 prep of,presence
R6171 T9275 T9276 det an,region
R6172 T9276 T9274 pobj region,of
R6173 T9277 T9276 amod unpaired,region
R6174 T9278 T9276 prep of,region
R6175 T9279 T9280 amod sufficient,length
R6176 T9280 T9278 pobj length,of
R6177 T9281 T9282 punct (,nt
R6178 T9282 T9280 parataxis nt,length
R6179 T9283 T9284 advmod at,least
R618 T1116 T1114 pobj structures,to
R6180 T9284 T9285 advmod least,40
R6181 T9285 T9282 nummod 40,nt
R6182 T9286 T9282 punct ),nt
R6183 T9287 T9276 punct ", ",region
R6184 T9288 T9289 dep which,permit
R6185 T9289 T9276 relcl permit,region
R6186 T9290 T9289 aux may,permit
R6187 T9291 T9289 dobj assembly,permit
R6188 T9292 T9291 prep of,assembly
R6189 T9293 T9294 det a,complex
R619 T1117 T1115 cc and,fork
R6190 T9294 T9292 pobj complex,of
R6191 T9295 T9296 amod double,hexameric
R6192 T9296 T9294 amod hexameric,complex
R6193 T9297 T9296 punct -,hexameric
R6194 T9298 T9294 prep on,complex
R6195 T9299 T9300 det the,DNA
R6196 T9300 T9298 pobj DNA,on
R6197 T9301 T9300 compound substrate,DNA
R6198 T9302 T9294 punct ", ",complex
R6199 T9303 T9294 amod similar,complex
R620 T1118 T1115 conj bubble,fork
R6200 T9304 T9303 prep to,similar
R6201 T9305 T9306 compound SV40,protein
R6202 T9306 T9304 pobj protein,to
R6203 T9307 T9308 compound T,antigen
R6204 T9308 T9306 compound antigen,protein
R6205 T9309 T9308 punct -,antigen
R6206 T9310 T9311 punct (,2A
R6207 T9311 T9304 parataxis 2A,to
R6208 T9312 T9311 compound Figure,2A
R6209 T9313 T9311 punct ),2A
R621 T1119 T1109 prep in,binds
R6210 T9314 T9315 punct (,21
R6211 T9315 T9289 parataxis 21,permit
R6212 T9316 T9315 punct ),21
R6213 T9317 T9253 punct .,indicate
R6214 T9319 T9320 advmod When,replaced
R6215 T9320 T9335 advcl replaced,reduced
R6216 T9321 T9322 nummod one,strand
R6217 T9322 T9320 nsubjpass strand,replaced
R6218 T9323 T9322 prep of,strand
R6219 T9324 T9325 det the,segment
R622 T1120 T1121 det an,manner
R6220 T9325 T9323 pobj segment,of
R6221 T9326 T9327 amod single,stranded
R6222 T9327 T9325 amod stranded,segment
R6223 T9328 T9327 punct -,stranded
R6224 T9329 T9322 prep in,strand
R6225 T9330 T9331 npadvmod T,rich
R6226 T9331 T9333 amod rich,bubble
R6227 T9332 T9331 punct -,rich
R6228 T9333 T9329 pobj bubble,in
R6229 T9334 T9320 auxpass was,replaced
R623 T1121 T1119 pobj manner,in
R6230 T9336 T9320 agent by,replaced
R6231 T9337 T9338 npadvmod guanine,rich
R6232 T9338 T9340 amod rich,sequences
R6233 T9339 T9338 punct -,rich
R6234 T9340 T9336 pobj sequences,by
R6235 T9341 T9335 punct ", ",reduced
R6236 T9342 T9343 det the,efficiency
R6237 T9343 T9335 nsubjpass efficiency,reduced
R6238 T9344 T9343 prep of,efficiency
R6239 T9345 T9344 pobj unwinding,of
R624 T1122 T1123 npadvmod ATP,dependent
R6240 T9346 T9335 auxpass was,reduced
R6241 T9347 T9335 advmod significantly,reduced
R6242 T9348 T9335 punct .,reduced
R6243 T9350 T9351 poss Our,analyses
R6244 T9351 T9353 nsubj analyses,showed
R6245 T9352 T9351 compound footprinting,analyses
R6246 T9354 T9355 mark that,protects
R6247 T9355 T9353 ccomp protects,showed
R6248 T9356 T9355 nsubj Mcm4,protects
R6249 T9357 T9356 punct /,Mcm4
R625 T1123 T1121 amod dependent,manner
R6250 T9358 T9356 nummod 6,Mcm4
R6251 T9359 T9356 punct /,Mcm4
R6252 T9360 T9356 nummod 7,Mcm4
R6253 T9361 T9355 advmod strongly,protects
R6254 T9362 T9363 nummod 25,nt
R6255 T9363 T9364 nmod nt,segment
R6256 T9364 T9355 dobj segment,protects
R6257 T9365 T9366 amod single,stranded
R6258 T9366 T9364 amod stranded,segment
R6259 T9367 T9366 punct -,stranded
R626 T1124 T1123 punct -,dependent
R6260 T9368 T9364 compound DNA,segment
R6261 T9369 T9364 amod adjacent,segment
R6262 T9370 T9369 prep to,adjacent
R6263 T9371 T9372 det each,point
R6264 T9372 T9370 pobj point,to
R6265 T9373 T9372 compound branch,point
R6266 T9374 T9369 cc and,adjacent
R6267 T9375 T9369 conj proximal,adjacent
R6268 T9376 T9375 prep to,proximal
R6269 T9377 T9378 nummod 5,ends
R627 T1125 T1109 punct ", ",binds
R6270 T9378 T9376 pobj ends,to
R6271 T9379 T9377 punct ′,5
R6272 T9380 T9378 prep of,ends
R6273 T9381 T9382 det both,strands
R6274 T9382 T9380 pobj strands,of
R6275 T9383 T9382 prep of,strands
R6276 T9384 T9385 det the,bubble
R6277 T9385 T9383 pobj bubble,of
R6278 T9386 T9353 punct .,showed
R6279 T9388 T9389 prep At,is
R628 T1126 T1109 cc and,binds
R6280 T9390 T9391 det each,fork
R6281 T9391 T9388 pobj fork,At
R6282 T9392 T9389 punct ", ",is
R6283 T9393 T9394 nummod one,complex
R6284 T9394 T9389 nsubj complex,is
R6285 T9395 T9394 nmod Mcm4,complex
R6286 T9396 T9395 punct /,Mcm4
R6287 T9397 T9395 nummod 6,Mcm4
R6288 T9398 T9395 punct /,Mcm4
R6289 T9399 T9395 nummod 7,Mcm4
R629 T1127 T1109 conj generates,binds
R6290 T9400 T9389 acomp likely,is
R6291 T9401 T9402 aux to,encircle
R6292 T9402 T9400 xcomp encircle,likely
R6293 T9403 T9404 det the,strand
R6294 T9404 T9402 dobj strand,encircle
R6295 T9405 T9406 amod single,stranded
R6296 T9406 T9404 amod stranded,strand
R6297 T9407 T9406 punct -,stranded
R6298 T9408 T9404 compound DNA,strand
R6299 T9409 T9389 cc and,is
R630 T1128 T1129 det a,complex
R6300 T9410 T9411 nummod two,hexamers
R6301 T9411 T9412 nsubj hexamers,bind
R6302 T9412 T9389 conj bind,is
R6303 T9413 T9412 aux may,bind
R6304 T9414 T9412 advmod symmetrically,bind
R6305 T9415 T9412 prep to,bind
R6306 T9416 T9417 det the,substrate
R6307 T9417 T9415 pobj substrate,to
R6308 T9418 T9417 compound bubble,substrate
R6309 T9419 T9412 punct ", ",bind
R631 T1129 T1127 dobj complex,generates
R6310 T9420 T9412 advcl forming,bind
R6311 T9421 T9422 det a,structure
R6312 T9422 T9420 dobj structure,forming
R6313 T9423 T9424 amod double,hexameric
R6314 T9424 T9422 amod hexameric,structure
R6315 T9425 T9420 prep on,forming
R6316 T9426 T9427 det the,bubble
R6317 T9427 T9425 pobj bubble,on
R6318 T9428 T9429 punct (,see
R6319 T9429 T9420 parataxis see,forming
R632 T1130 T1131 amod double,hexameric
R6320 T9430 T9431 amod schematic,drawing
R6321 T9431 T9429 dobj drawing,see
R6322 T9432 T9431 prep of,drawing
R6323 T9433 T9434 compound Figure,1A
R6324 T9434 T9432 pobj 1A,of
R6325 T9435 T9429 punct ),see
R6326 T9436 T9412 punct .,bind
R6327 T9438 T9439 amod Efficient,unwinding
R6328 T9439 T9440 nsubj unwinding,require
R6329 T9441 T9439 prep into,unwinding
R633 T1131 T1129 amod hexameric,complex
R6330 T9442 T9443 det both,directions
R6331 T9443 T9441 pobj directions,into
R6332 T9444 T9440 aux may,require
R6333 T9445 T9446 amod simultaneous,activation
R6334 T9446 T9440 dobj activation,require
R6335 T9447 T9446 prep of,activation
R6336 T9448 T9449 det both,hexamers
R6337 T9449 T9447 pobj hexamers,of
R6338 T9450 T9451 dep which,sit
R6339 T9451 T9449 relcl sit,hexamers
R634 T1132 T1131 punct -,hexameric
R6340 T9452 T9451 aux may,sit
R6341 T9453 T9451 prep at,sit
R6342 T9454 T9455 det the,center
R6343 T9455 T9453 pobj center,at
R6344 T9456 T9457 mark while,extruding
R6345 T9457 T9451 advcl extruding,sit
R6346 T9458 T9459 det the,DNA
R6347 T9459 T9457 dobj DNA,extruding
R6348 T9460 T9459 amod unwound,DNA
R6349 T9461 T9462 amod single,stranded
R635 T1133 T1127 punct ", ",generates
R6350 T9462 T9459 amod stranded,DNA
R6351 T9463 T9462 punct -,stranded
R6352 T9464 T9457 prep through,extruding
R6353 T9465 T9466 det the,rings
R6354 T9466 T9464 pobj rings,through
R6355 T9467 T9440 punct .,require
R6356 T9469 T9470 nsubj This,resemble
R6357 T9471 T9470 aux may,resemble
R6358 T9472 T9470 advmod closely,resemble
R6359 T9473 T9474 det the,modes
R636 T1134 T1135 mark as,shown
R6360 T9474 T9470 dobj modes,resemble
R6361 T9475 T9474 amod proposed,modes
R6362 T9476 T9474 prep of,modes
R6363 T9477 T9478 nmod binding,actions
R6364 T9478 T9476 pobj actions,of
R6365 T9479 T9477 cc and,binding
R6366 T9480 T9477 conj helicase,binding
R6367 T9481 T9478 prep of,actions
R6368 T9482 T9483 compound T,antigen
R6369 T9483 T9481 pobj antigen,of
R637 T1135 T1127 advcl shown,generates
R6370 T9484 T9483 punct -,antigen
R6371 T9485 T9486 punct (,21
R6372 T9486 T9470 parataxis 21,resemble
R6373 T9487 T9486 punct ),21
R6374 T9488 T9470 punct .,resemble
R6375 T9490 T9491 mark Although,exclude
R6376 T9491 T9496 advcl exclude,bear
R6377 T9492 T9491 nsubj we,exclude
R6378 T9493 T9491 aux can,exclude
R6379 T9494 T9491 neg not,exclude
R638 T1136 T1135 auxpass was,shown
R6380 T9495 T9491 advmod totally,exclude
R6381 T9497 T9498 det the,possibility
R6382 T9498 T9491 dobj possibility,exclude
R6383 T9499 T9500 mark that,unwinds
R6384 T9500 T9498 acl unwinds,possibility
R6385 T9501 T9502 nummod one,hexamer
R6386 T9502 T9500 nsubj hexamer,unwinds
R6387 T9503 T9502 nmod Mcm4,hexamer
R6388 T9504 T9503 punct /,Mcm4
R6389 T9505 T9503 nummod 6,Mcm4
R639 T1137 T1135 prep for,shown
R6390 T9506 T9503 punct /,Mcm4
R6391 T9507 T9503 nummod 7,Mcm4
R6392 T9508 T9502 prep at,hexamer
R6393 T9509 T9510 det each,fork
R6394 T9510 T9508 pobj fork,at
R6395 T9511 T9512 det the,duplex
R6396 T9512 T9500 dobj duplex,unwinds
R6397 T9513 T9500 advmod independently,unwinds
R6398 T9514 T9496 punct ", ",bear
R6399 T9515 T9516 npadvmod ring,shaped
R640 T1138 T1139 compound T,antigen
R6400 T9516 T9518 amod shaped,structures
R6401 T9517 T9516 punct -,shaped
R6402 T9518 T9496 nsubj structures,bear
R6403 T9519 T9518 prep of,structures
R6404 T9520 T9521 nmod mouse,MCM
R6405 T9521 T9519 pobj MCM,of
R6406 T9522 T9520 cc and,mouse
R6407 T9523 T9520 conj archea,mouse
R6408 T9524 T9496 punct ", ",bear
R6409 T9525 T9526 mark as,revealed
R641 T1139 T1137 pobj antigen,for
R6410 T9526 T9496 advcl revealed,bear
R6411 T9527 T9526 agent by,revealed
R6412 T9528 T9529 compound electron,microscopy
R6413 T9529 T9527 pobj microscopy,by
R6414 T9530 T9496 punct ", ",bear
R6415 T9531 T9532 amod much,similarity
R6416 T9532 T9496 dobj similarity,bear
R6417 T9533 T9532 prep to,similarity
R6418 T9534 T9535 det the,structure
R6419 T9535 T9533 pobj structure,to
R642 T1140 T1139 punct -,antigen
R6420 T9536 T9537 advmod recently,solved
R6421 T9537 T9535 amod solved,structure
R6422 T9538 T9535 prep of,structure
R6423 T9539 T9540 det the,antigen
R6424 T9540 T9538 pobj antigen,of
R6425 T9541 T9540 nmod SV40,antigen
R6426 T9542 T9540 amod large,antigen
R6427 T9543 T9540 compound T,antigen
R6428 T9544 T9540 punct -,antigen
R6429 T9545 T9546 punct (,6
R643 T1141 T1142 punct (,21
R6430 T9546 T9535 parataxis 6,structure
R6431 T9547 T9546 nummod 4,6
R6432 T9548 T9546 punct ",",6
R6433 T9549 T9546 punct ),6
R6434 T9550 T9496 punct ", ",bear
R6435 T9551 T9496 cc and,bear
R6436 T9552 T9496 conj are,bear
R6437 T9553 T9552 prep in,are
R6438 T9554 T9553 pobj favor,in
R6439 T9555 T9554 prep of,favor
R644 T1142 T1135 parataxis 21,shown
R6440 T9556 T9557 det the,structure
R6441 T9557 T9555 pobj structure,of
R6442 T9558 T9559 amod double,hexameric
R6443 T9559 T9557 amod hexameric,structure
R6444 T9560 T9559 punct -,hexameric
R6445 T9561 T9557 prep of,structure
R6446 T9562 T9563 compound mouse,Mcm
R6447 T9563 T9561 pobj Mcm,of
R6448 T9564 T9557 prep on,structure
R6449 T9565 T9566 det a,DNA
R645 T1143 T1142 nummod 13,21
R6450 T9566 T9564 pobj DNA,on
R6451 T9567 T9566 compound bubble,DNA
R6452 T9568 T9496 punct .,bear
R6455 T9856 T9857 nmod Substrate,requirement
R6456 T9858 T9856 cc and,Substrate
R6457 T9859 T9856 conj sequence,Substrate
R6458 T9860 T9857 prep for,requirement
R6459 T9861 T9862 nmod Mcm4,activation
R646 T1144 T1142 punct ",",21
R6460 T9862 T9860 pobj activation,for
R6461 T9863 T9861 punct /,Mcm4
R6462 T9864 T9861 nummod 6,Mcm4
R6463 T9865 T9861 punct /,Mcm4
R6464 T9866 T9861 nummod 7,Mcm4
R6465 T9867 T9862 compound helicase,activation
R6466 T9869 T9870 compound DNA,binding
R6467 T9870 T9871 compound binding,assays
R6468 T9871 T9872 nsubj assays,indicate
R6469 T9873 T9874 mark that,binds
R647 T1145 T1142 nummod 14,21
R6470 T9874 T9872 ccomp binds,indicate
R6471 T9875 T9874 nsubj Mcm4,binds
R6472 T9876 T9875 punct /,Mcm4
R6473 T9877 T9875 nummod 6,Mcm4
R6474 T9878 T9875 punct /,Mcm4
R6475 T9879 T9875 nummod 7,Mcm4
R6476 T9880 T9874 prep to,binds
R6477 T9881 T9882 det those,substrates
R6478 T9882 T9880 pobj substrates,to
R6479 T9883 T9882 acl containing,substrates
R648 T1146 T1142 punct ",",21
R6480 T9884 T9885 amod single,stranded
R6481 T9885 T9887 amod stranded,regions
R6482 T9886 T9885 punct -,stranded
R6483 T9887 T9883 dobj regions,containing
R6484 T9888 T9887 compound DNA,regions
R6485 T9889 T9874 advmod regardless,binds
R6486 T9890 T9889 prep of,regardless
R6487 T9891 T9892 det the,presence
R6488 T9892 T9890 pobj presence,of
R6489 T9893 T9892 cc or,presence
R649 T1147 T1142 punct ),21
R6490 T9894 T9892 conj absence,presence
R6491 T9895 T9892 prep of,presence
R6492 T9896 T9897 amod single,stranded
R6493 T9897 T9899 amod stranded,end
R6494 T9898 T9897 punct -,stranded
R6495 T9899 T9895 pobj end,of
R6496 T9900 T9899 nummod 3,end
R6497 T9901 T9900 punct ′,3
R6498 T9902 T9900 cc or,3
R6499 T9903 T9900 conj 5,3
R650 T1148 T1109 punct .,binds
R6500 T9904 T9903 punct ′,5
R6501 T9905 T9872 punct .,indicate
R6502 T9907 T9908 nsubj Unwinding,depends
R6503 T9909 T9907 prep of,Unwinding
R6504 T9910 T9911 det the,DNA
R6505 T9911 T9909 pobj DNA,of
R6506 T9912 T9911 compound duplex,DNA
R6507 T9913 T9908 prep on,depends
R6508 T9914 T9913 pobj translocation,on
R6509 T9915 T9914 prep of,translocation
R651 T1150 T1151 advmod Recently,reported
R6510 T9916 T9917 amod single,stranded
R6511 T9917 T9919 amod stranded,DNA
R6512 T9918 T9917 punct -,stranded
R6513 T9919 T9915 pobj DNA,of
R6514 T9920 T9914 prep from,translocation
R6515 T9921 T9920 pobj 3,from
R6516 T9922 T9921 punct ′,3
R6517 T9923 T9920 prep to,from
R6518 T9924 T9923 pobj 5,to
R6519 T9925 T9924 punct ′,5
R652 T1152 T1151 punct ", ",reported
R6520 T9926 T9920 pobj direction,from
R6521 T9927 T9908 punct .,depends
R6522 T9929 T9930 nsubjpass This,shown
R6523 T9931 T9930 auxpass is,shown
R6524 T9932 T9933 advmod most,clearly
R6525 T9933 T9930 advmod clearly,shown
R6526 T9934 T9930 prep by,shown
R6527 T9935 T9936 poss its,action
R6528 T9936 T9934 pobj action,by
R6529 T9937 T9936 compound helicase,action
R653 T1153 T1151 nsubj we,reported
R6530 T9938 T9936 prep on,action
R6531 T9939 T9940 nmod A,fork
R6532 T9940 T9942 nmod fork,extension
R6533 T9941 T9940 punct -,fork
R6534 T9942 T9938 pobj extension,on
R6535 T9943 T9940 punct [,fork
R6536 T9944 T9940 appos 5,fork
R6537 T9945 T9944 punct ′,5
R6538 T9946 T9944 punct ],5
R6539 T9947 T9944 cc and,5
R654 T1154 T1155 mark that,activated
R6540 T9948 T9944 conj 3,5
R6541 T9949 T9948 punct ′,3
R6542 T9950 T9942 punct -,extension
R6543 T9951 T9938 cc but,on
R6544 T9952 T9951 neg not,but
R6545 T9953 T9938 conj on,on
R6546 T9954 T9955 nmod A,fork
R6547 T9955 T9957 nmod fork,extension
R6548 T9956 T9955 punct -,fork
R6549 T9957 T9953 pobj extension,on
R655 T1155 T1151 ccomp activated,reported
R6550 T9958 T9955 punct [,fork
R6551 T9959 T9955 nummod 3,fork
R6552 T9960 T9955 punct ′,fork
R6553 T9961 T9955 punct ],fork
R6554 T9962 T9955 punct ", ",fork
R6555 T9963 T9964 compound A,fork
R6556 T9964 T9955 conj fork,fork
R6557 T9965 T9964 punct -,fork
R6558 T9966 T9964 punct [,fork
R6559 T9967 T9964 appos 3,fork
R656 T1156 T1157 det the,activities
R6560 T9968 T9967 punct ′,3
R6561 T9969 T9967 punct ",",3
R6562 T9970 T9967 appos 5,3
R6563 T9971 T9970 punct ′,5
R6564 T9972 T9964 punct ],fork
R6565 T9973 T9964 cc nor,fork
R6566 T9974 T9964 conj 5,fork
R6567 T9975 T9974 punct ′,5
R6568 T9976 T9957 punct -,extension
R6569 T9977 T9930 punct .,shown
R657 T1157 T1155 nsubjpass activities,activated
R6570 T9979 T9980 advmod However,require
R6571 T9981 T9980 punct ", ",require
R6572 T9982 T9980 nsubj it,require
R6573 T9983 T9980 aux does,require
R6574 T9984 T9980 neg not,require
R6575 T9985 T9980 advmod necessarily,require
R6576 T9986 T9987 nummod 3,end
R6577 T9987 T9980 dobj end,require
R6578 T9988 T9986 punct ′,3
R6579 T9989 T9987 prep of,end
R658 T1158 T1157 nmod helicase,activities
R6580 T9990 T9991 amod single,stranded
R6581 T9991 T9993 amod stranded,DNA
R6582 T9992 T9991 punct -,stranded
R6583 T9993 T9989 pobj DNA,of
R6584 T9994 T9980 punct ", ",require
R6585 T9995 T9996 mark since,displace
R6586 T9996 T9980 advcl displace,require
R6587 T9997 T9996 nsubj Mcm4,displace
R6588 T9998 T9997 punct /,Mcm4
R6589 T9999 T9997 nummod 6,Mcm4
R659 T1159 T1158 cc and,helicase
R6590 T10000 T9997 punct /,Mcm4
R6591 T10001 T9997 nummod 7,Mcm4
R6592 T10002 T9996 aux can,displace
R6593 T10003 T10004 amod annealing,oligonucleotide
R6594 T10004 T9996 dobj oligonucleotide,displace
R6595 T10005 T9996 prep on,displace
R6596 T10006 T10007 det a,DNA
R6597 T10007 T10005 pobj DNA,on
R6598 T10008 T10007 amod circular,DNA
R6599 T10009 T10010 amod single,stranded
R660 T1160 T1161 compound ATP,hydrolysis
R6600 T10010 T10007 amod stranded,DNA
R6601 T10011 T10010 punct -,stranded
R6602 T10012 T9980 punct .,require
R6603 T10014 T10015 det The,ability
R6604 T10015 T10016 nsubjpass ability,shared
R6605 T10017 T10015 prep of,ability
R6606 T10018 T10019 det the,Mcm4
R6607 T10019 T10017 pobj Mcm4,of
R6608 T10020 T10019 compound mouse,Mcm4
R6609 T10021 T10019 punct /,Mcm4
R661 T1161 T1158 conj hydrolysis,helicase
R6610 T10022 T10019 nummod 6,Mcm4
R6611 T10023 T10019 punct /,Mcm4
R6612 T10024 T10019 nummod 7,Mcm4
R6613 T10025 T10026 aux to,unwind
R6614 T10026 T10015 acl unwind,ability
R6615 T10027 T10028 nummod 3,extension
R6616 T10028 T10026 dobj extension,unwind
R6617 T10029 T10027 punct ′,3
R6618 T10030 T10028 punct -,extension
R6619 T10031 T10016 auxpass is,shared
R662 T1162 T1157 prep of,activities
R6620 T10032 T10016 prep by,shared
R6621 T10033 T10034 det the,helicase
R6622 T10034 T10032 pobj helicase,by
R6623 T10035 T10034 amod archaeal,helicase
R6624 T10036 T10034 compound Mcm,helicase
R6625 T10037 T10032 cc but,by
R6626 T10038 T10037 neg not,but
R6627 T10039 T10032 conj by,by
R6628 T10040 T10039 pobj Mcm4,by
R6629 T10041 T10040 punct /,Mcm4
R663 T1163 T1164 amod mammalian,Mcm4
R6630 T10042 T10040 nummod 6,Mcm4
R6631 T10043 T10040 punct /,Mcm4
R6632 T10044 T10040 nummod 7,Mcm4
R6633 T10045 T10040 prep from,Mcm4
R6634 T10046 T10045 pobj S.pombe,from
R6635 T10047 T10046 cc and,S.pombe
R6636 T10048 T10046 conj S.cerevisiae,S.pombe
R6637 T10049 T10050 punct (,27
R6638 T10050 T10016 parataxis 27,shared
R6639 T10051 T10050 nummod 14,27
R664 T1164 T1162 pobj Mcm4,of
R6640 T10052 T10050 punct ",",27
R6641 T10053 T10050 nummod 26,27
R6642 T10054 T10050 punct ",",27
R6643 T10055 T10050 punct ),27
R6644 T10056 T10016 punct .,shared
R6645 T10058 T10059 det The,Mcm
R6646 T10059 T10061 nsubj Mcm,unwind
R6647 T10060 T10059 amod archaeal,Mcm
R6648 T10062 T10061 aux can,unwind
R6649 T10063 T10064 compound A,fork
R665 T1165 T1164 punct /,Mcm4
R6650 T10064 T10061 dobj fork,unwind
R6651 T10065 T10064 punct -,fork
R6652 T10066 T10064 punct [,fork
R6653 T10067 T10064 nummod 3,fork
R6654 T10068 T10064 punct ′,fork
R6655 T10069 T10061 punct ],unwind
R6656 T10070 T10061 cc but,unwind
R6657 T10071 T10072 amod eukaryotic,Mcm4
R6658 T10072 T10073 nsubj Mcm4,can
R6659 T10073 T10061 conj can,unwind
R666 T1166 T1164 nummod 6,Mcm4
R6660 T10074 T10072 punct /,Mcm4
R6661 T10075 T10072 nummod 6,Mcm4
R6662 T10076 T10072 punct /,Mcm4
R6663 T10077 T10072 nummod 7,Mcm4
R6664 T10078 T10073 neg not,can
R6665 T10079 T10061 punct ", ",unwind
R6666 T10080 T10081 mark since,binds
R6667 T10081 T10061 advcl binds,unwind
R6668 T10082 T10083 det the,former
R6669 T10083 T10081 nsubj former,binds
R667 T1167 T1164 punct /,Mcm4
R6670 T10084 T10081 prep to,binds
R6671 T10085 T10086 amod double,stranded
R6672 T10086 T10088 amod stranded,DNA
R6673 T10087 T10086 punct -,stranded
R6674 T10088 T10084 pobj DNA,to
R6675 T10089 T10081 cc but,binds
R6676 T10090 T10091 det the,latter
R6677 T10091 T10092 nsubj latter,27
R6678 T10092 T10081 conj 27,binds
R6679 T10093 T10092 aux does,27
R668 T1168 T1164 nummod 7,Mcm4
R6680 T10094 T10092 neg not,27
R6681 T10095 T10092 punct (,27
R6682 T10096 T10092 nummod 5,27
R6683 T10097 T10092 punct ",",27
R6684 T10098 T10092 punct ),27
R6685 T10099 T10061 punct .,unwind
R6686 T10101 T10102 mark While,translocate
R6687 T10102 T10107 advcl translocate,observed
R6688 T10103 T10104 compound yeast,Mcm
R6689 T10104 T10105 compound Mcm,helicases
R669 T1169 T1155 auxpass are,activated
R6690 T10105 T10102 nsubj helicases,translocate
R6691 T10106 T10102 aux can,translocate
R6692 T10108 T10102 prep on,translocate
R6693 T10109 T10110 compound duplex,DNA
R6694 T10110 T10108 pobj DNA,on
R6695 T10111 T10107 punct ", ",observed
R6696 T10112 T10113 amod such,activity
R6697 T10113 T10107 nsubjpass activity,observed
R6698 T10114 T10107 auxpass was,observed
R6699 T10115 T10107 neg not,observed
R670 T1170 T1155 advmod specifically,activated
R6700 T10116 T10107 prep with,observed
R6701 T10117 T10118 amod mammalian,Mcm4
R6702 T10118 T10116 pobj Mcm4,with
R6703 T10119 T10118 punct /,Mcm4
R6704 T10120 T10118 nummod 6,Mcm4
R6705 T10121 T10118 punct /,Mcm4
R6706 T10122 T10118 nummod 7,Mcm4
R6707 T10123 T10124 punct (,shown
R6708 T10124 T10107 parataxis shown,observed
R6709 T10125 T10124 nsubj data,shown
R671 T1171 T1155 agent by,activated
R6710 T10126 T10124 neg not,shown
R6711 T10127 T10124 punct ),shown
R6712 T10128 T10107 punct .,observed
R6713 T10130 T10131 nsubj Occurrence,lead
R6714 T10132 T10130 prep of,Occurrence
R6715 T10133 T10134 npadvmod AT,rich
R6716 T10134 T10136 amod rich,sequences
R6717 T10135 T10134 punct -,rich
R6718 T10136 T10132 pobj sequences,of
R6719 T10137 T10130 punct ", ",Occurrence
R672 T1172 T1173 amod single,stranded
R6720 T10138 T10130 prep with,Occurrence
R6721 T10139 T10140 amod asymmetric,distribution
R6722 T10140 T10138 pobj distribution,with
R6723 T10141 T10140 prep of,distribution
R6724 T10142 T10141 pobj adenine,of
R6725 T10143 T10142 cc and,adenine
R6726 T10144 T10142 conj thymine,adenine
R6727 T10145 T10140 punct ", ",distribution
R6728 T10146 T10140 prep near,distribution
R6729 T10147 T10148 det the,origins
R673 T1173 T1175 amod stranded,DNA
R6730 T10148 T10146 pobj origins,near
R6731 T10149 T10148 compound replication,origins
R6732 T10150 T10131 punct ", ",lead
R6733 T10151 T10131 dobj us,lead
R6734 T10152 T10153 aux to,propose
R6735 T10153 T10131 xcomp propose,lead
R6736 T10154 T10155 mark that,play
R6737 T10155 T10153 ccomp play,propose
R6738 T10156 T10155 nsubj Mcm,play
R6739 T10157 T10155 aux may,play
R674 T1174 T1173 punct -,stranded
R6740 T10158 T10159 det a,role
R6741 T10159 T10155 dobj role,play
R6742 T10160 T10155 prep in,play
R6743 T10161 T10160 pobj selection,in
R6744 T10162 T10161 prep of,selection
R6745 T10163 T10164 compound initiation,sites
R6746 T10164 T10162 pobj sites,of
R6747 T10165 T10164 prep of,sites
R6748 T10166 T10167 amod mammalian,DNA
R6749 T10167 T10168 compound DNA,replication
R675 T1175 T1171 pobj DNA,by
R6750 T10168 T10165 pobj replication,of
R6751 T10169 T10131 punct ", ",lead
R6752 T10170 T10131 cc and,lead
R6753 T10171 T10131 conj prompted,lead
R6754 T10172 T10171 dobj us,prompted
R6755 T10173 T10174 aux to,examine
R6756 T10174 T10171 xcomp examine,prompted
R6757 T10175 T10176 det the,ability
R6758 T10176 T10174 dobj ability,examine
R6759 T10177 T10176 prep of,ability
R676 T1176 T1175 acl containing,DNA
R6760 T10178 T10177 pobj sequences,of
R6761 T10179 T10178 prep from,sequences
R6762 T10180 T10181 amod human,origins
R6763 T10181 T10179 pobj origins,from
R6764 T10182 T10181 compound replication,origins
R6765 T10183 T10184 aux to,activate
R6766 T10184 T10176 acl activate,ability
R6767 T10185 T10186 nmod Mcm4,helicase
R6768 T10186 T10184 dobj helicase,activate
R6769 T10187 T10185 punct /,Mcm4
R677 T1177 T1176 dobj stretches,containing
R6770 T10188 T10185 nummod 6,Mcm4
R6771 T10189 T10185 punct /,Mcm4
R6772 T10190 T10185 nummod 7,Mcm4
R6773 T10191 T10131 punct .,lead
R6774 T10193 T10194 preconj Both,B2
R6775 T10194 T10197 nmod B2,origins
R6776 T10195 T10194 nmod lamin,B2
R6777 T10196 T10194 punct -,B2
R6778 T10197 T10202 nsubj origins,served
R6779 T10198 T10194 cc and,B2
R678 T1178 T1177 prep of,stretches
R6780 T10199 T10200 compound c,myc
R6781 T10200 T10194 conj myc,B2
R6782 T10201 T10200 punct -,myc
R6783 T10203 T10202 prep as,served
R6784 T10204 T10205 amod efficient,activator
R6785 T10205 T10203 pobj activator,as
R6786 T10206 T10205 prep for,activator
R6787 T10207 T10208 compound Mcm,helicase
R6788 T10208 T10206 pobj helicase,for
R6789 T10209 T10210 advmod in,vitro
R679 T1179 T1180 compound thymine,residues
R6790 T10210 T10202 advmod vitro,served
R6791 T10211 T10202 punct .,served
R6792 T10213 T10214 advcl Consistent,reported
R6793 T10215 T10213 prep with,Consistent
R6794 T10216 T10215 pobj it,with
R6795 T10217 T10214 punct ", ",reported
R6796 T10218 T10219 npadvmod site,specific
R6797 T10219 T10221 amod specific,loading
R6798 T10220 T10219 punct -,specific
R6799 T10221 T10214 nsubjpass loading,reported
R680 T1180 T1178 pobj residues,of
R6800 T10222 T10221 prep of,loading
R6801 T10223 T10222 pobj Mcm,of
R6802 T10224 T10221 prep in,loading
R6803 T10225 T10226 det the,zone
R6804 T10226 T10224 pobj zone,in
R6805 T10227 T10228 compound DNA,initiation
R6806 T10228 T10226 compound initiation,zone
R6807 T10229 T10228 compound replication,initiation
R6808 T10230 T10226 prep of,zone
R6809 T10231 T10232 det the,myc
R681 T1181 T1151 cc and,reported
R6810 T10232 T10230 pobj myc,of
R6811 T10233 T10232 compound c,myc
R6812 T10234 T10232 punct -,myc
R6813 T10235 T10214 auxpass was,reported
R6814 T10236 T10214 advmod recently,reported
R6815 T10237 T10238 punct (,33
R6816 T10238 T10214 parataxis 33,reported
R6817 T10239 T10238 punct ),33
R6818 T10240 T10214 punct .,reported
R6819 T10242 T10243 nsubj We,examined
R682 T1182 T1151 conj proposed,reported
R6820 T10244 T10243 aux have,examined
R6821 T10245 T10243 prep in,examined
R6822 T10246 T10245 pobj detail,in
R6823 T10247 T10248 det the,effect
R6824 T10248 T10243 dobj effect,examined
R6825 T10249 T10248 prep of,effect
R6826 T10250 T10251 compound sequence,context
R6827 T10251 T10249 pobj context,of
R6828 T10252 T10251 prep of,context
R6829 T10253 T10254 det the,DNA
R683 T1183 T1184 det a,model
R6830 T10254 T10252 pobj DNA,of
R6831 T10255 T10256 amod single,stranded
R6832 T10256 T10254 amod stranded,DNA
R6833 T10257 T10256 punct -,stranded
R6834 T10258 T10248 prep on,effect
R6835 T10259 T10260 det the,activity
R6836 T10260 T10258 pobj activity,on
R6837 T10261 T10260 compound helicase,activity
R6838 T10262 T10260 prep of,activity
R6839 T10263 T10262 pobj Mcm,of
R684 T1184 T1182 dobj model,proposed
R6840 T10264 T10243 punct .,examined
R6841 T10266 T10267 det The,results
R6842 T10267 T10268 nsubj results,indicate
R6843 T10269 T10270 mark that,is
R6844 T10270 T10268 ccomp is,indicate
R6845 T10271 T10272 compound thymine,content
R6846 T10272 T10270 nsubj content,is
R6847 T10273 T10272 prep of,content
R6848 T10274 T10275 nummod 50,%
R6849 T10275 T10273 pobj %,of
R685 T1185 T1184 amod novel,model
R6850 T10276 T10270 acomp sufficient,is
R6851 T10277 T10276 prep for,sufficient
R6852 T10278 T10279 det the,activity
R6853 T10279 T10277 pobj activity,for
R6854 T10280 T10279 amod maximum,activity
R6855 T10281 T10279 compound helicase,activity
R6856 T10282 T10268 punct .,indicate
R6857 T10284 T10285 det The,efficiency
R6858 T10285 T10286 nsubj efficiency,decreased
R6859 T10287 T10285 prep of,efficiency
R686 T1186 T1187 mark that,play
R6860 T10288 T10287 pobj displacement,of
R6861 T10289 T10290 mark as,dropped
R6862 T10290 T10286 advcl dropped,decreased
R6863 T10291 T10292 det the,content
R6864 T10292 T10290 nsubj content,dropped
R6865 T10293 T10292 compound thymine,content
R6866 T10294 T10292 prep of,content
R6867 T10295 T10296 det the,tail
R6868 T10296 T10294 pobj tail,of
R6869 T10297 T10296 nummod 3,tail
R687 T1187 T1184 acl play,model
R6870 T10298 T10297 punct ′,3
R6871 T10299 T10296 punct -,tail
R6872 T10300 T10290 prep to,dropped
R6873 T10301 T10302 nummod 33,%
R6874 T10302 T10300 pobj %,to
R6875 T10303 T10304 punct (,Figure
R6876 T10304 T10290 parataxis Figure,dropped
R6877 T10305 T10304 nummod 6,Figure
R6878 T10306 T10304 punct ),Figure
R6879 T10307 T10286 punct .,decreased
R688 T1188 T1187 nsubj Mcm,play
R6880 T10309 T10310 det The,stretches
R6881 T10310 T10311 nsubjpass stretches,required
R6882 T10312 T10310 prep of,stretches
R6883 T10313 T10314 compound thymine,residues
R6884 T10314 T10312 pobj residues,of
R6885 T10315 T10311 aux may,required
R6886 T10316 T10311 neg not,required
R6887 T10317 T10311 auxpass be,required
R6888 T10318 T10311 advmod necessarily,required
R6889 T10319 T10311 punct ", ",required
R689 T1189 T1187 aux may,play
R6890 T10320 T10321 mark since,served
R6891 T10321 T10311 advcl served,required
R6892 T10322 T10321 nsubj repeats,served
R6893 T10323 T10322 prep of,repeats
R6894 T10324 T10325 compound TC,dinucleotides
R6895 T10325 T10323 pobj dinucleotides,of
R6896 T10326 T10321 prep as,served
R6897 T10327 T10328 det a,activator
R6898 T10328 T10326 pobj activator,as
R6899 T10329 T10328 amod potent,activator
R690 T1190 T1191 det a,role
R6900 T10330 T10328 prep for,activator
R6901 T10331 T10330 pobj Mcm4,for
R6902 T10332 T10331 punct /,Mcm4
R6903 T10333 T10331 nummod 6,Mcm4
R6904 T10334 T10331 punct /,Mcm4
R6905 T10335 T10331 nummod 7,Mcm4
R6906 T10336 T10311 punct .,required
R6907 T10338 T10339 nsubj We,noticed
R6908 T10340 T10339 advmod also,noticed
R6909 T10341 T10342 mark that,is
R691 T1191 T1187 dobj role,play
R6910 T10342 T10339 ccomp is,noticed
R6911 T10343 T10344 det the,presence
R6912 T10344 T10342 nsubj presence,is
R6913 T10345 T10344 prep of,presence
R6914 T10346 T10347 det a,structure
R6915 T10347 T10345 pobj structure,of
R6916 T10348 T10347 amod secondary,structure
R6917 T10349 T10347 prep within,structure
R6918 T10350 T10351 det the,DNA
R6919 T10351 T10349 pobj DNA,within
R692 T1192 T1191 amod crucial,role
R6920 T10352 T10353 amod single,stranded
R6921 T10353 T10351 amod stranded,DNA
R6922 T10354 T10353 punct -,stranded
R6923 T10355 T10342 acomp inhibitory,is
R6924 T10356 T10355 prep for,inhibitory
R6925 T10357 T10358 compound helicase,action
R6926 T10358 T10356 pobj action,for
R6927 T10359 T10339 punct .,noticed
R6928 T10361 T10362 compound Nuclease,footprinting
R6929 T10362 T10363 compound footprinting,assays
R693 T1193 T1187 prep in,play
R6930 T10363 T10364 nsubj assays,indicated
R6931 T10365 T10366 mark that,interfered
R6932 T10366 T10364 ccomp interfered,indicated
R6933 T10367 T10366 nsubjpass binding,interfered
R6934 T10368 T10366 auxpass was,interfered
R6935 T10369 T10366 agent by,interfered
R6936 T10370 T10371 det the,structure
R6937 T10371 T10369 pobj structure,by
R6938 T10372 T10371 amod secondary,structure
R6939 T10373 T10374 punct (,shown
R694 T1194 T1193 pobj selection,in
R6940 T10374 T10366 parataxis shown,interfered
R6941 T10375 T10374 nsubj data,shown
R6942 T10376 T10374 neg not,shown
R6943 T10377 T10374 punct ),shown
R6944 T10378 T10364 punct .,indicated
R6945 T10380 T10381 advmod Thus,concluded
R6946 T10382 T10381 punct ", ",concluded
R6947 T10383 T10381 nsubj we,concluded
R6948 T10384 T10381 aux have,concluded
R6949 T10385 T10386 mark that,activated
R695 T1195 T1194 prep of,selection
R6950 T10386 T10381 ccomp activated,concluded
R6951 T10387 T10388 nmod Mcm4,helicase
R6952 T10388 T10386 nsubjpass helicase,activated
R6953 T10389 T10387 punct /,Mcm4
R6954 T10390 T10387 nummod 6,Mcm4
R6955 T10391 T10387 punct /,Mcm4
R6956 T10392 T10387 nummod 7,Mcm4
R6957 T10393 T10386 auxpass is,activated
R6958 T10394 T10395 advmod most,efficiently
R6959 T10395 T10386 advmod efficiently,activated
R696 T1196 T1197 compound replication,origins
R6960 T10396 T10386 agent by,activated
R6961 T10397 T10398 amod non-structured,DNA
R6962 T10398 T10396 pobj DNA,by
R6963 T10399 T10400 amod single,stranded
R6964 T10400 T10398 amod stranded,DNA
R6965 T10401 T10400 punct -,stranded
R6966 T10402 T10398 prep with,DNA
R6967 T10403 T10404 compound thymine,content
R6968 T10404 T10402 pobj content,with
R6969 T10405 T10404 prep of,content
R697 T1197 T1195 pobj origins,of
R6970 T10406 T10407 nummod 50,%
R6971 T10407 T10405 pobj %,of
R6972 T10408 T10409 cc or,more
R6973 T10409 T10407 nummod more,%
R6974 T10410 T10386 punct ", ",activated
R6975 T10411 T10412 mark although,observed
R6976 T10412 T10386 advcl observed,activated
R6977 T10413 T10414 amod significant,stimulation
R6978 T10414 T10412 nsubjpass stimulation,observed
R6979 T10415 T10412 auxpass is,observed
R698 T1198 T1187 prep in,play
R6980 T10416 T10412 advmod also,observed
R6981 T10417 T10412 agent by,observed
R6982 T10418 T10417 pobj DNA,by
R6983 T10419 T10418 prep with,DNA
R6984 T10420 T10421 amod less,content
R6985 T10421 T10419 pobj content,with
R6986 T10422 T10421 compound thymine,content
R6987 T10423 T10424 punct (,Figure
R6988 T10424 T10418 parataxis Figure,DNA
R6989 T10425 T10424 nummod 7,Figure
R699 T1199 T1200 amod higher,eukaryotes
R6990 T10426 T10424 punct ),Figure
R6991 T10427 T10412 punct ", ",observed
R6992 T10428 T10412 advcl suggesting,observed
R6993 T10429 T10430 mark that,is
R6994 T10430 T10428 advcl is,suggesting
R6995 T10431 T10432 det the,specificity
R6996 T10432 T10430 nsubj specificity,is
R6997 T10433 T10432 compound sequence,specificity
R6998 T10434 T10432 prep for,specificity
R6999 T10435 T10436 compound Mcm,helicase
R700 T1200 T1198 pobj eukaryotes,in
R7000 T10436 T10437 compound helicase,activation
R7001 T10437 T10434 pobj activation,for
R7002 T10438 T10439 advmod rather,relaxed
R7003 T10439 T10430 acomp relaxed,is
R7004 T10440 T10430 cc and,is
R7005 T10441 T10442 mark that,depend
R7006 T10442 T10430 conj depend,is
R7007 T10443 T10444 det the,extent
R7008 T10444 T10442 nsubj extent,depend
R7009 T10445 T10444 prep of,extent
R701 T1201 T1202 punct (,13
R7010 T10446 T10447 det the,activation
R7011 T10447 T10445 pobj activation,of
R7012 T10448 T10442 aux may,depend
R7013 T10449 T10442 prep on,depend
R7014 T10450 T10451 det the,context
R7015 T10451 T10449 pobj context,on
R7016 T10452 T10451 compound sequence,context
R7017 T10453 T10381 punct .,concluded
R7018 T10455 T10456 nsubj This,be
R7019 T10457 T10456 aux would,be
R702 T1202 T1182 parataxis 13,proposed
R7020 T10458 T10456 acomp reasonable,be
R7021 T10459 T10456 prep given,be
R7022 T10460 T10461 det the,flexibility
R7023 T10461 T10459 pobj flexibility,given
R7024 T10462 T10461 cc and,flexibility
R7025 T10463 T10464 amod differential,regulation
R7026 T10464 T10461 conj regulation,flexibility
R7027 T10465 T10461 prep of,flexibility
R7028 T10466 T10467 compound site,selection
R7029 T10467 T10465 pobj selection,of
R703 T1203 T1202 punct ),13
R7030 T10468 T10461 prep for,flexibility
R7031 T10469 T10468 pobj initiation,for
R7032 T10470 T10469 prep of,initiation
R7033 T10471 T10472 compound DNA,replication
R7034 T10472 T10470 pobj replication,of
R7035 T10473 T10469 prep during,initiation
R7036 T10474 T10473 pobj development,during
R7037 T10475 T10468 cc or,for
R7038 T10476 T10468 conj in,for
R7039 T10477 T10478 amod various,types
R704 T1204 T1151 punct .,reported
R7040 T10478 T10476 pobj types,in
R7041 T10479 T10478 compound cell,types
R7042 T10480 T10461 punct ", ",flexibility
R7043 T10481 T10482 advmod as,as
R7044 T10482 T10461 cc as,flexibility
R7045 T10483 T10482 advmod well,as
R7046 T10484 T10485 det the,variability
R7047 T10485 T10461 conj variability,flexibility
R7048 T10486 T10485 prep in,variability
R7049 T10487 T10488 compound initiation,potential
R705 T1206 T1207 prep In,conducted
R7050 T10488 T10486 pobj potential,in
R7051 T10489 T10488 prep of,potential
R7052 T10490 T10491 det each,origin
R7053 T10491 T10489 pobj origin,of
R7054 T10492 T10491 compound replication,origin
R7055 T10493 T10491 prep on,origin
R7056 T10494 T10495 det the,genome
R7057 T10495 T10493 pobj genome,on
R7058 T10496 T10497 advmod even,within
R7059 T10497 T10485 prep within,variability
R706 T1208 T1209 det this,report
R7060 T10498 T10499 det the,type
R7061 T10499 T10497 pobj type,within
R7062 T10500 T10499 amod single,type
R7063 T10501 T10499 compound cell,type
R7064 T10502 T10456 punct .,be
R7067 T10855 T10856 nmod Mcm4,helicase
R7068 T10857 T10855 punct /,Mcm4
R7069 T10858 T10855 nummod 6,Mcm4
R707 T1209 T1206 pobj report,In
R7070 T10859 T10855 punct /,Mcm4
R7071 T10860 T10855 nummod 7,Mcm4
R7072 T10861 T10856 prep during,helicase
R7073 T10862 T10863 amod processive,unwinding
R7074 T10863 T10861 pobj unwinding,during
R7075 T10864 T10856 prep at,helicase
R7076 T10865 T10866 det the,fork
R7077 T10866 T10864 pobj fork,at
R7078 T10868 T10869 det The,requirement
R7079 T10869 T10871 nsubj requirement,prompted
R708 T1210 T1207 punct ", ",conducted
R7080 T10870 T10869 amod specific,requirement
R7081 T10872 T10869 prep of,requirement
R7082 T10873 T10874 amod single,stranded
R7083 T10874 T10876 amod stranded,residues
R7084 T10875 T10874 punct -,stranded
R7085 T10876 T10872 pobj residues,of
R7086 T10877 T10876 compound thymine,residues
R7087 T10878 T10869 prep for,requirement
R7088 T10879 T10878 pobj activation,for
R7089 T10880 T10879 prep of,activation
R709 T1211 T1207 prep in,conducted
R7090 T10881 T10882 compound Mcm,helicase
R7091 T10882 T10880 pobj helicase,of
R7092 T10883 T10871 dobj us,prompted
R7093 T10884 T10885 aux to,examine
R7094 T10885 T10871 xcomp examine,prompted
R7095 T10886 T10887 mark whether,required
R7096 T10887 T10885 ccomp required,examine
R7097 T10888 T10887 nsubjpass they,required
R7098 T10889 T10887 auxpass are,required
R7099 T10890 T10887 advmod also,required
R710 T1212 T1211 pobj order,in
R7100 T10891 T10887 prep for,required
R7101 T10892 T10893 amod processive,unwinding
R7102 T10893 T10891 pobj unwinding,for
R7103 T10894 T10893 prep of,unwinding
R7104 T10895 T10896 compound duplex,DNA
R7105 T10896 T10894 pobj DNA,of
R7106 T10897 T10871 punct .,prompted
R7107 T10899 T10900 poss Our,results
R7108 T10900 T10901 nsubj results,indicated
R7109 T10902 T10903 mark that,inhibited
R711 T1213 T1214 aux to,clarify
R7110 T10903 T10901 ccomp inhibited,indicated
R7111 T10904 T10903 nsubj increase,inhibited
R7112 T10905 T10904 prep of,increase
R7113 T10906 T10907 compound GC,pair
R7114 T10907 T10905 pobj pair,of
R7115 T10908 T10904 prep in,increase
R7116 T10909 T10910 det the,segment
R7117 T10910 T10908 pobj segment,in
R7118 T10911 T10910 compound duplex,segment
R7119 T10912 T10903 advmod significantly,inhibited
R712 T1214 T1212 acl clarify,order
R7120 T10913 T10914 det the,activity
R7121 T10914 T10903 dobj activity,inhibited
R7122 T10915 T10916 compound Mcm,helicase
R7123 T10916 T10914 compound helicase,activity
R7124 T10917 T10901 punct .,indicated
R7125 T10919 T10920 compound Duplex,DNA
R7126 T10920 T10921 nsubjpass DNA,displaced
R7127 T10922 T10920 acl composed,DNA
R7128 T10923 T10924 advmod only,of
R7129 T10924 T10922 prep of,composed
R713 T1215 T1216 det the,mode
R7130 T10925 T10926 compound GC,pairs
R7131 T10926 T10924 pobj pairs,of
R7132 T10927 T10928 punct (,repeats
R7133 T10928 T10926 parataxis repeats,pairs
R7134 T10929 T10928 nummod 10,repeats
R7135 T10930 T10928 prep of,repeats
R7136 T10931 T10930 pobj CGG,of
R7137 T10932 T10928 punct ),repeats
R7138 T10933 T10926 prep on,pairs
R7139 T10934 T10935 det a,fork
R714 T1216 T1214 dobj mode,clarify
R7140 T10935 T10933 pobj fork,on
R7141 T10936 T10935 compound Y,fork
R7142 T10937 T10935 punct -,fork
R7143 T10938 T10921 auxpass was,displaced
R7144 T10939 T10921 neg not,displaced
R7145 T10940 T10941 advmod at,all
R7146 T10941 T10921 advmod all,displaced
R7147 T10942 T10921 punct ", ",displaced
R7148 T10943 T10944 mark while,displaced
R7149 T10944 T10921 advcl displaced,displaced
R715 T1217 T1216 prep of,mode
R7150 T10945 T10946 det the,template
R7151 T10946 T10944 nsubjpass template,displaced
R7152 T10947 T10946 amod same,template
R7153 T10948 T10944 auxpass was,displaced
R7154 T10949 T10944 advmod readily,displaced
R7155 T10950 T10944 agent by,displaced
R7156 T10951 T10952 compound SV40,antigen
R7157 T10952 T10950 pobj antigen,by
R7158 T10953 T10952 compound T,antigen
R7159 T10954 T10952 punct -,antigen
R716 T1218 T1217 pobj action,of
R7160 T10955 T10956 punct (,8B
R7161 T10956 T10944 parataxis 8B,displaced
R7162 T10957 T10956 compound Figure,8B
R7163 T10958 T10956 punct ),8B
R7164 T10959 T10921 punct .,displaced
R7165 T10961 T10962 compound Mcm,helicase
R7166 T10962 T10963 nsubjpass helicase,inhibited
R7167 T10964 T10963 auxpass was,inhibited
R7168 T10965 T10963 agent by,inhibited
R7169 T10966 T10967 det the,presence
R717 T1219 T1216 prep of,mode
R7170 T10967 T10965 pobj presence,by
R7171 T10968 T10967 prep of,presence
R7172 T10969 T10970 npadvmod GC,rich
R7173 T10970 T10972 amod rich,segment
R7174 T10971 T10970 punct -,rich
R7175 T10972 T10968 pobj segment,of
R7176 T10973 T10972 compound duplex,segment
R7177 T10974 T10975 advmod also,on
R7178 T10975 T10967 prep on,presence
R7179 T10976 T10977 det a,substrate
R718 T1220 T1221 det the,helicase
R7180 T10977 T10975 pobj substrate,on
R7181 T10978 T10977 amod circular,substrate
R7182 T10979 T10980 amod single,stranded
R7183 T10980 T10977 amod stranded,substrate
R7184 T10981 T10980 punct -,stranded
R7185 T10982 T10983 amod partial,heteroduplex
R7186 T10983 T10977 compound heteroduplex,substrate
R7187 T10984 T10963 punct .,inhibited
R7188 T10986 T10987 advmod However,was
R7189 T10988 T10987 punct ", ",was
R719 T1221 T1219 pobj helicase,of
R7190 T10989 T10987 prep on,was
R7191 T10990 T10991 det this,substrate
R7192 T10991 T10989 pobj substrate,on
R7193 T10992 T10987 punct ", ",was
R7194 T10993 T10987 nsubj Mcm4,was
R7195 T10994 T10993 punct /,Mcm4
R7196 T10995 T10993 nummod 6,Mcm4
R7197 T10996 T10993 punct /,Mcm4
R7198 T10997 T10993 nummod 7,Mcm4
R7199 T10998 T10987 acomp able,was
R720 T1222 T1221 compound Mcm,helicase
R7200 T10999 T11000 aux to,displace
R7201 T11000 T10998 xcomp displace,able
R7202 T11001 T11000 dobj DNA,displace
R7203 T11002 T11000 prep past,displace
R7204 T11003 T11004 det the,region
R7205 T11004 T11002 pobj region,past
R7206 T11005 T11006 npadvmod GC,rich
R7207 T11006 T11004 amod rich,region
R7208 T11007 T11006 punct -,rich
R7209 T11008 T11000 punct ", ",displace
R721 T1223 T1214 cc and,clarify
R7210 T11009 T11010 dep albeit,to
R7211 T11010 T11000 prep to,displace
R7212 T11011 T11012 det a,extent
R7213 T11012 T11010 pobj extent,to
R7214 T11013 T11012 amod limited,extent
R7215 T11014 T11000 punct ", ",displace
R7216 T11015 T11016 advmod when,added
R7217 T11016 T11000 advcl added,displace
R7218 T11017 T11016 nsubjpass it,added
R7219 T11018 T11016 auxpass was,added
R722 T1224 T1214 conj obtain,clarify
R7220 T11019 T11016 prep at,added
R7221 T11020 T11021 det a,concentration
R7222 T11021 T11019 pobj concentration,at
R7223 T11022 T11021 amod high,concentration
R7224 T11023 T10987 punct .,was
R7225 T11025 T11026 prep On,loaded
R7226 T11027 T11028 det the,template
R7227 T11028 T11025 pobj template,On
R7228 T11029 T11030 amod partial,heteroduplex
R7229 T11030 T11028 compound heteroduplex,template
R723 T1225 T1224 dobj insight,obtain
R7230 T11031 T11026 punct ", ",loaded
R7231 T11032 T11026 nsubjpass Mcm,loaded
R7232 T11033 T11026 auxpass is,loaded
R7233 T11034 T11026 prep onto,loaded
R7234 T11035 T11036 det the,DNA
R7235 T11036 T11034 pobj DNA,onto
R7236 T11037 T11036 amod circular,DNA
R7237 T11038 T11039 amod single,stranded
R7238 T11039 T11036 amod stranded,DNA
R7239 T11040 T11039 punct -,stranded
R724 T1226 T1225 prep into,insight
R7240 T11041 T11036 prep of,DNA
R7241 T11042 T11043 nummod 6.4,kb
R7242 T11043 T11041 pobj kb,of
R7243 T11044 T11026 punct ", ",loaded
R7244 T11045 T11046 mark while,loaded
R7245 T11046 T11026 advcl loaded,loaded
R7246 T11047 T11046 nsubjpass it,loaded
R7247 T11048 T11046 auxpass is,loaded
R7248 T11049 T11046 prep onto,loaded
R7249 T11050 T11051 det the,DNA
R725 T1227 T1228 advmod how,function
R7250 T11051 T11049 pobj DNA,onto
R7251 T11052 T11053 nummod 50,nt
R7252 T11053 T11054 npadvmod nt,long
R7253 T11054 T11051 amod long,DNA
R7254 T11055 T11056 nummod 3,tail
R7255 T11056 T11051 compound tail,DNA
R7256 T11057 T11055 punct ′,3
R7257 T11058 T11056 punct -,tail
R7258 T11059 T11051 prep on,DNA
R7259 T11060 T11061 det the,fork
R726 T1228 T1226 pcomp function,into
R7260 T11061 T11059 pobj fork,on
R7261 T11062 T11061 compound Y,fork
R7262 T11063 T11061 punct -,fork
R7263 T11064 T11026 punct .,loaded
R7264 T11066 T11067 advmod Thus,reflect
R7265 T11068 T11067 punct ", ",reflect
R7266 T11069 T11070 det the,difference
R7267 T11070 T11067 nsubj difference,reflect
R7268 T11071 T11070 prep of,difference
R7269 T11072 T11073 compound helicase,actions
R727 T1229 T1228 nsubj it,function
R7270 T11073 T11071 pobj actions,of
R7271 T11074 T11067 aux may,reflect
R7272 T11075 T11076 det the,efficiency
R7273 T11076 T11067 dobj efficiency,reflect
R7274 T11077 T11076 prep of,efficiency
R7275 T11078 T11079 compound Mcm,loading
R7276 T11079 T11077 pobj loading,of
R7277 T11080 T11067 punct .,reflect
R7278 T11082 T11083 advmod Alternatively,engage
R7279 T11084 T11083 punct ", ",engage
R728 T1230 T1228 aux may,function
R7280 T11085 T11086 det the,presence
R7281 T11086 T11083 nsubj presence,engage
R7282 T11087 T11086 prep of,presence
R7283 T11088 T11087 punct ‘,of
R7284 T11089 T11090 amod random,sequence
R7285 T11090 T11087 pobj sequence,of
R7286 T11091 T11090 punct ’,sequence
R7287 T11092 T11086 prep at,presence
R7288 T11093 T11094 det the,segment
R7289 T11094 T11092 pobj segment,at
R729 T1231 T1228 prep at,function
R7290 T11095 T11096 advmod initially,unwound
R7291 T11096 T11094 amod unwound,segment
R7292 T11097 T11094 compound duplex,segment
R7293 T11098 T11094 prep in,segment
R7294 T11099 T11100 det the,template
R7295 T11100 T11098 pobj template,in
R7296 T11101 T11100 amod former,template
R7297 T11102 T11083 aux may,engage
R7298 T11103 T11104 det the,helicase
R7299 T11104 T11083 dobj helicase,engage
R730 T1232 T1233 det the,forks
R7300 T11105 T11104 compound Mcm,helicase
R7301 T11106 T11083 prep in,engage
R7302 T11107 T11108 det a,conformation
R7303 T11108 T11106 pobj conformation,in
R7304 T11109 T11110 advmod more,active
R7305 T11110 T11108 amod active,conformation
R7306 T11111 T11112 dep which,displace
R7307 T11112 T11108 relcl displace,conformation
R7308 T11113 T11112 aux can,displace
R7309 T11114 T11115 det the,segment
R731 T1233 T1231 pobj forks,at
R7310 T11115 T11112 dobj segment,displace
R7311 T11116 T11117 npadvmod GC,rich
R7312 T11117 T11115 amod rich,segment
R7313 T11118 T11117 punct -,rich
R7314 T11119 T11115 compound duplex,segment
R7315 T11120 T11083 punct .,engage
R7316 T11122 T11123 prep On,restored
R7317 T11124 T11125 compound Y,fork
R7318 T11125 T11127 compound fork,substrates
R7319 T11126 T11125 punct -,fork
R732 T1234 T1233 compound replication,forks
R7320 T11127 T11122 pobj substrates,On
R7321 T11128 T11123 punct ", ",restored
R7322 T11129 T11123 nsubj increase,restored
R7323 T11130 T11129 prep of,increase
R7324 T11131 T11132 compound AT,pairs
R7325 T11132 T11130 pobj pairs,of
R7326 T11133 T11132 compound base,pairs
R7327 T11134 T11129 prep in,increase
R7328 T11135 T11136 det the,duplex
R7329 T11136 T11134 pobj duplex,in
R733 T1235 T1236 advmod in,vivo
R7330 T11137 T11138 punct (,repeats
R7331 T11138 T11136 parataxis repeats,duplex
R7332 T11139 T11138 nummod 10,repeats
R7333 T11140 T11138 prep of,repeats
R7334 T11141 T11140 pobj CTT,of
R7335 T11142 T11138 punct ),repeats
R7336 T11143 T11144 det the,unwinding
R7337 T11144 T11123 dobj unwinding,restored
R7338 T11145 T11123 punct .,restored
R7339 T11147 T11148 det These,results
R734 T1236 T1228 advmod vivo,function
R7340 T11148 T11149 nsubj results,indicate
R7341 T11150 T11151 preconj either,required
R7342 T11151 T11149 advcl required,indicate
R7343 T11152 T11151 mark that,required
R7344 T11153 T11154 compound thymine,residues
R7345 T11154 T11151 nsubjpass residues,required
R7346 T11155 T11151 auxpass are,required
R7347 T11156 T11151 prep on,required
R7348 T11157 T11158 det the,DNA
R7349 T11158 T11156 pobj DNA,on
R735 T1237 T1207 punct ", ",conducted
R7350 T11159 T11158 compound duplex,DNA
R7351 T11160 T11151 prep for,required
R7352 T11161 T11162 amod continuous,unwinding
R7353 T11162 T11160 pobj unwinding,for
R7354 T11163 T11151 punct ", ",required
R7355 T11164 T11151 cc or,required
R7356 T11165 T11166 mark that,is
R7357 T11166 T11151 conj is,required
R7358 T11167 T11166 nsubj Mcm4,is
R7359 T11168 T11167 punct /,Mcm4
R736 T1238 T1207 nsubj we,conducted
R7360 T11169 T11167 nummod 6,Mcm4
R7361 T11170 T11167 punct /,Mcm4
R7362 T11171 T11167 nummod 7,Mcm4
R7363 T11172 T11166 advmod simply,is
R7364 T11173 T11166 neg not,is
R7365 T11174 T11166 acomp efficient,is
R7366 T11175 T11174 advmod enough,efficient
R7367 T11176 T11177 aux to,unwind
R7368 T11177 T11174 xcomp unwind,efficient
R7369 T11178 T11179 det the,pairs
R737 T1239 T1207 aux have,conducted
R7370 T11179 T11177 dobj pairs,unwind
R7371 T11180 T11179 amod stable,pairs
R7372 T11181 T11179 compound GC,pairs
R7373 T11182 T11149 punct .,indicate
R7374 T11184 T11185 nsubj Replacement,resulted
R7375 T11186 T11184 prep of,Replacement
R7376 T11187 T11188 det the,guanosine
R7377 T11188 T11186 pobj guanosine,of
R7378 T11189 T11188 amod central,guanosine
R7379 T11190 T11184 prep with,Replacement
R738 T1240 T1241 amod detailed,analyses
R7380 T11191 T11190 pobj inosine,with
R7381 T11192 T11184 prep in,Replacement
R7382 T11193 T11194 det the,DNA
R7383 T11194 T11192 pobj DNA,in
R7384 T11195 T11196 compound CGG,repeat
R7385 T11196 T11194 compound repeat,DNA
R7386 T11197 T11194 compound duplex,DNA
R7387 T11198 T11185 prep in,resulted
R7388 T11199 T11198 pobj displacement,in
R7389 T11200 T11201 punct (,8E
R739 T1241 T1207 dobj analyses,conducted
R7390 T11201 T11185 parataxis 8E,resulted
R7391 T11202 T11201 compound Figure,8E
R7392 T11203 T11201 punct ),8E
R7393 T11204 T11205 punct (,31
R7394 T11205 T11185 parataxis 31,resulted
R7395 T11206 T11205 punct ),31
R7396 T11207 T11185 punct ", ",resulted
R7397 T11208 T11185 advcl suggesting,resulted
R7398 T11209 T11210 mark that,be
R7399 T11210 T11208 advcl be,suggesting
R740 T1242 T1241 prep of,analyses
R7400 T11211 T11212 det the,presence
R7401 T11212 T11210 nsubj presence,be
R7402 T11213 T11212 amod continuous,presence
R7403 T11214 T11212 prep of,presence
R7404 T11215 T11216 compound AT,pair
R7405 T11216 T11214 pobj pair,of
R7406 T11217 T11216 compound base,pair
R7407 T11218 T11210 aux may,be
R7408 T11219 T11210 neg not,be
R7409 T11220 T11210 acomp essential,be
R741 T1243 T1244 compound helicase,action
R7410 T11221 T11220 prep for,essential
R7411 T11222 T11223 det the,function
R7412 T11223 T11221 pobj function,for
R7413 T11224 T11225 compound Mcm,helicase
R7414 T11225 T11223 compound helicase,function
R7415 T11226 T11210 cc and,be
R7416 T11227 T11228 mark that,is
R7417 T11228 T11210 conj is,be
R7418 T11229 T11230 det the,thermostability
R7419 T11230 T11228 nsubj thermostability,is
R742 T1244 T1242 pobj action,of
R7420 T11231 T11230 prep of,thermostability
R7421 T11232 T11233 compound GC,pairs
R7422 T11233 T11231 pobj pairs,of
R7423 T11234 T11233 compound base,pairs
R7424 T11235 T11228 acomp inhibitory,is
R7425 T11236 T11235 prep for,inhibitory
R7426 T11237 T11238 compound helicase,action
R7427 T11238 T11236 pobj action,for
R7428 T11239 T11238 prep of,action
R7429 T11240 T11239 pobj Mcm4,of
R743 T1245 T1244 cc and,action
R7430 T11241 T11240 punct /,Mcm4
R7431 T11242 T11240 nummod 6,Mcm4
R7432 T11243 T11240 punct /,Mcm4
R7433 T11244 T11240 nummod 7,Mcm4
R7434 T11245 T11185 punct .,resulted
R7435 T11247 T11248 det The,results
R7436 T11248 T11249 nsubj results,reveal
R7437 T11250 T11248 acl described,results
R7438 T11251 T11250 prep in,described
R7439 T11252 T11253 det this,manuscript
R744 T1246 T1247 compound DNA,binding
R7440 T11253 T11251 pobj manuscript,in
R7441 T11254 T11255 advmod potentially,important
R7442 T11255 T11256 amod important,features
R7443 T11256 T11249 dobj features,reveal
R7444 T11257 T11256 prep of,features
R7445 T11258 T11259 amod mammalian,helicase
R7446 T11259 T11257 pobj helicase,of
R7447 T11260 T11259 nmod Mcm4,helicase
R7448 T11261 T11260 punct /,Mcm4
R7449 T11262 T11260 nummod 6,Mcm4
R745 T1247 T1244 conj binding,action
R7450 T11263 T11260 punct /,Mcm4
R7451 T11264 T11260 nummod 7,Mcm4
R7452 T11265 T11259 punct ", ",helicase
R7453 T11266 T11267 dep which,is
R7454 T11267 T11259 relcl is,helicase
R7455 T11268 T11267 acomp likely,is
R7456 T11269 T11270 aux to,be
R7457 T11270 T11268 xcomp be,likely
R7458 T11271 T11272 det a,component
R7459 T11272 T11270 attr component,be
R746 T1248 T1244 prep of,action
R7460 T11273 T11272 amod key,component
R7461 T11274 T11272 prep of,component
R7462 T11275 T11276 det the,helicase
R7463 T11276 T11274 pobj helicase,of
R7464 T11277 T11276 amod eukaryotic,helicase
R7465 T11278 T11276 amod replicative,helicase
R7466 T11279 T11249 punct .,reveal
R7467 T11281 T11282 amod Prior,to
R7468 T11282 T11283 prep to,adopt
R7469 T11284 T11282 pobj initiation,to
R747 T1249 T1250 nmod mouse,helicase
R7470 T11285 T11284 prep of,initiation
R7471 T11286 T11287 compound DNA,replication
R7472 T11287 T11285 pobj replication,of
R7473 T11288 T11283 punct ", ",adopt
R7474 T11289 T11290 compound Mcm,helicase
R7475 T11290 T11283 nsubj helicase,adopt
R7476 T11291 T11283 aux may,adopt
R7477 T11292 T11293 det a,complex
R7478 T11293 T11283 dobj complex,adopt
R7479 T11294 T11295 amod double,hexameric
R748 T1250 T1248 pobj helicase,of
R7480 T11295 T11293 amod hexameric,complex
R7481 T11296 T11283 prep at,adopt
R7482 T11297 T11298 det the,region
R7483 T11298 T11296 pobj region,at
R7484 T11299 T11300 advmod partially,melted
R7485 T11300 T11298 amod melted,region
R7486 T11301 T11298 compound origin,region
R7487 T11302 T11283 punct ", ",adopt
R7488 T11303 T11283 cc and,adopt
R7489 T11304 T11305 aux may,catalyze
R749 T1251 T1250 nmod Mcm4,helicase
R7490 T11305 T11283 conj catalyze,adopt
R7491 T11306 T11307 amod concurrent,unwinding
R7492 T11307 T11305 dobj unwinding,catalyze
R7493 T11308 T11307 prep of,unwinding
R7494 T11309 T11310 compound duplex,DNA
R7495 T11310 T11308 pobj DNA,of
R7496 T11311 T11305 prep into,catalyze
R7497 T11312 T11313 det both,directions
R7498 T11313 T11311 pobj directions,into
R7499 T11314 T11283 punct ", ",adopt
R750 T1252 T1251 punct /,Mcm4
R7500 T11315 T11316 mark while,associated
R7501 T11316 T11283 advcl associated,adopt
R7502 T11317 T11316 advmod stably,associated
R7503 T11318 T11316 prep with,associated
R7504 T11319 T11320 det the,origins
R7505 T11320 T11318 pobj origins,with
R7506 T11321 T11320 prep of,origins
R7507 T11322 T11323 compound DNA,replication
R7508 T11323 T11321 pobj replication,of
R7509 T11324 T11283 punct .,adopt
R751 T1253 T1251 nummod 6,Mcm4
R7510 T11326 T11327 nsubj This,is
R7511 T11328 T11327 acomp similar,is
R7512 T11329 T11328 prep to,similar
R7513 T11330 T11331 det the,model
R7514 T11331 T11329 pobj model,to
R7515 T11332 T11333 compound T,antigen
R7516 T11333 T11331 compound antigen,model
R7517 T11334 T11333 punct -,antigen
R7518 T11335 T11336 advmod originally,proposed
R7519 T11336 T11331 acl proposed,model
R752 T1254 T1251 punct /,Mcm4
R7520 T11337 T11336 agent by,proposed
R7521 T11338 T11337 pobj Smelkova,by
R7522 T11339 T11338 cc and,Smelkova
R7523 T11340 T11338 conj Borowiec,Smelkova
R7524 T11341 T11342 punct (,21
R7525 T11342 T11340 parataxis 21,Borowiec
R7526 T11343 T11342 punct ),21
R7527 T11344 T11327 punct .,is
R7528 T11346 T11347 nsubj We,propose
R7529 T11348 T11349 mark that,mobilized
R753 T1255 T1251 nummod 7,Mcm4
R7530 T11349 T11347 ccomp mobilized,propose
R7531 T11350 T11349 punct ", ",mobilized
R7532 T11351 T11352 advmod only,activated
R7533 T11352 T11349 advcl activated,mobilized
R7534 T11353 T11352 advmod when,activated
R7535 T11354 T11355 det both,hexamers
R7536 T11355 T11352 nsubjpass hexamers,activated
R7537 T11356 T11352 auxpass are,activated
R7538 T11357 T11352 agent by,activated
R7539 T11358 T11359 det the,sequences
R754 T1256 T1207 advcl using,conducted
R7540 T11359 T11357 pobj sequences,by
R7541 T11360 T11359 amod interacting,sequences
R7542 T11361 T11362 npadvmod thymine,rich
R7543 T11362 T11359 amod rich,sequences
R7544 T11363 T11362 punct -,rich
R7545 T11364 T11359 relcl present,sequences
R7546 T11365 T11364 prep within,present
R7547 T11366 T11367 det the,region
R7548 T11367 T11365 pobj region,within
R7549 T11368 T11367 amod melted,region
R755 T1257 T1258 amod various,DNAs
R7550 T11369 T11349 punct ", ",mobilized
R7551 T11370 T11371 det the,helicase
R7552 T11371 T11349 nsubjpass helicase,mobilized
R7553 T11372 T11371 compound Mcm,helicase
R7554 T11373 T11349 auxpass is,mobilized
R7555 T11374 T11349 cc and,mobilized
R7556 T11375 T11376 nsubj initiation,takes
R7557 T11376 T11349 conj takes,mobilized
R7558 T11377 T11376 dobj place,takes
R7559 T11378 T11347 punct .,propose
R756 T1258 T1256 dobj DNAs,using
R7560 T11380 T11381 det A,question
R7561 T11381 T11383 nsubj question,is
R7562 T11382 T11381 amod crucial,question
R7563 T11384 T11385 advmod how,generated
R7564 T11385 T11383 ccomp generated,is
R7565 T11386 T11387 det these,complexes
R7566 T11387 T11385 nsubjpass complexes,generated
R7567 T11388 T11389 advmod double,hexameric
R7568 T11389 T11387 amod hexameric,complexes
R7569 T11390 T11387 compound helicase,complexes
R757 T1259 T1258 amod forked,DNAs
R7570 T11391 T11385 auxpass are,generated
R7571 T11392 T11385 cc and,generated
R7572 T11393 T11385 conj turn,generated
R7573 T11394 T11393 prep into,turn
R7574 T11395 T11396 amod active,helicases
R7575 T11396 T11394 pobj helicases,into
R7576 T11397 T11393 prep at,turn
R7577 T11398 T11399 det the,origins
R7578 T11399 T11397 pobj origins,at
R7579 T11400 T11383 punct .,is
R758 T1260 T1259 cc and,forked
R7580 T11402 T11403 det This,process
R7581 T11403 T11404 nsubj process,involve
R7582 T11405 T11404 aux would,involve
R7583 T11406 T11404 dobj melting,involve
R7584 T11407 T11406 prep of,melting
R7585 T11408 T11409 compound duplex,DNA
R7586 T11409 T11407 pobj DNA,of
R7587 T11410 T11406 punct ", ",melting
R7588 T11411 T11412 dep which,facilitated
R7589 T11412 T11406 relcl facilitated,melting
R759 T1261 T1262 compound bubble,substrate
R7590 T11413 T11412 aux may,facilitated
R7591 T11414 T11412 auxpass be,facilitated
R7592 T11415 T11412 prep by,facilitated
R7593 T11416 T11415 pobj binding,by
R7594 T11417 T11416 prep of,binding
R7595 T11418 T11419 compound preRC,components
R7596 T11419 T11417 pobj components,of
R7597 T11420 T11416 prep in,binding
R7598 T11421 T11422 det the,context
R7599 T11422 T11420 pobj context,in
R760 T1262 T1259 conj substrate,forked
R7600 T11423 T11422 prep of,context
R7601 T11424 T11425 amod proper,structures
R7602 T11425 T11423 pobj structures,of
R7603 T11426 T11425 compound chromatin,structures
R7604 T11427 T11415 punct ", ",by
R7605 T11428 T11415 cc or,by
R7606 T11429 T11415 conj by,by
R7607 T11430 T11431 amod other,factors
R7608 T11431 T11429 pobj factors,by
R7609 T11432 T11431 amod unwinding,factors
R761 T1263 T1207 punct .,conducted
R7610 T11433 T11431 prep including,factors
R7611 T11434 T11435 det a,topoisomerase
R7612 T11435 T11433 pobj topoisomerase,including
R7613 T11436 T11435 cc or,topoisomerase
R7614 T11437 T11438 det a,helicase
R7615 T11438 T11435 conj helicase,topoisomerase
R7616 T11439 T11440 advmod newly,identified
R7617 T11440 T11438 amod identified,helicase
R7618 T11441 T11438 compound Mcm8,helicase
R7619 T11442 T11443 punct (,34
R762 T1265 T1266 nsubj We,addressed
R7620 T11443 T11404 parataxis 34,involve
R7621 T11444 T11445 punct –,36
R7622 T11445 T11443 prep 36,34
R7623 T11446 T11443 punct ),34
R7624 T11447 T11404 punct .,involve
R7625 T11449 T11450 nsubj It,require
R7626 T11451 T11450 aux may,require
R7627 T11452 T11450 advmod also,require
R7628 T11453 T11450 dobj association,require
R7629 T11454 T11453 prep of,association
R763 T1267 T1266 advmod specifically,addressed
R7630 T11455 T11456 npadvmod fork,assisting
R7631 T11456 T11458 amod assisting,proteins
R7632 T11457 T11456 punct -,assisting
R7633 T11458 T11454 pobj proteins,of
R7634 T11459 T11460 amod such,as
R7635 T11460 T11458 prep as,proteins
R7636 T11461 T11460 pobj Cdc45,as
R7637 T11462 T11463 punct (,38
R7638 T11463 T11461 parataxis 38,Cdc45
R7639 T11464 T11463 nummod 37,38
R764 T1268 T1269 compound sequence,requirement
R7640 T11465 T11463 punct ",",38
R7641 T11466 T11463 punct ),38
R7642 T11467 T11468 advmod as,as
R7643 T11468 T11458 cc as,proteins
R7644 T11469 T11468 advmod well,as
R7645 T11470 T11471 compound phosphorylation,events
R7646 T11471 T11458 conj events,proteins
R7647 T11472 T11471 prep by,events
R7648 T11473 T11474 nmod Cdk,kinases
R7649 T11474 T11472 pobj kinases,by
R765 T1269 T1266 dobj requirement,addressed
R7650 T11475 T11473 cc and,Cdk
R7651 T11476 T11473 conj Cdc7,Cdk
R7652 T11477 T11478 punct (,40
R7653 T11478 T11474 parataxis 40,kinases
R7654 T11479 T11478 nummod 39,40
R7655 T11480 T11478 punct ",",40
R7656 T11481 T11478 punct ),40
R7657 T11482 T11450 punct .,require
R7658 T11484 T11485 nummod One,finding
R7659 T11485 T11487 nsubj finding,is
R766 T1270 T1269 prep for,requirement
R7660 T11486 T11485 amod unexpected,finding
R7661 T11488 T11485 prep of,finding
R7662 T11489 T11490 det this,study
R7663 T11490 T11488 pobj study,of
R7664 T11491 T11492 amod low,activity
R7665 T11492 T11487 attr activity,is
R7666 T11493 T11492 compound helicase,activity
R7667 T11494 T11492 prep of,activity
R7668 T11495 T11494 pobj Mcm4,of
R7669 T11496 T11495 punct /,Mcm4
R767 T1271 T1272 det the,activation
R7670 T11497 T11495 nummod 6,Mcm4
R7671 T11498 T11495 punct /,Mcm4
R7672 T11499 T11495 nummod 7,Mcm4
R7673 T11500 T11492 prep on,activity
R7674 T11501 T11502 npadvmod GC,rich
R7675 T11502 T11504 amod rich,DNA
R7676 T11503 T11502 punct -,rich
R7677 T11504 T11500 pobj DNA,on
R7678 T11505 T11504 compound duplex,DNA
R7679 T11506 T11487 punct .,is
R768 T1272 T1270 pobj activation,for
R7680 T11508 T11509 nsubj This,indicates
R7681 T11510 T11508 punct ", ",This
R7682 T11511 T11508 prep in,This
R7683 T11512 T11511 pobj conjunction,in
R7684 T11513 T11512 prep with,conjunction
R7685 T11514 T11515 det the,processivity
R7686 T11515 T11513 pobj processivity,with
R7687 T11516 T11515 amod low,processivity
R7688 T11517 T11515 prep of,processivity
R7689 T11518 T11519 nmod Mcm4,helicase
R769 T1273 T1274 compound Mcm,helicase
R7690 T11519 T11517 pobj helicase,of
R7691 T11520 T11518 punct /,Mcm4
R7692 T11521 T11518 nummod 6,Mcm4
R7693 T11522 T11518 punct /,Mcm4
R7694 T11523 T11518 nummod 7,Mcm4
R7695 T11524 T11509 punct ", ",indicates
R7696 T11525 T11509 advmod strongly,indicates
R7697 T11526 T11527 mark that,require
R7698 T11527 T11509 ccomp require,indicates
R7699 T11528 T11529 det the,helicase
R770 T1274 T1272 compound helicase,activation
R7700 T11529 T11527 nsubj helicase,require
R7701 T11530 T11529 amod replicative,helicase
R7702 T11531 T11529 prep at,helicase
R7703 T11532 T11533 det the,forks
R7704 T11533 T11531 pobj forks,at
R7705 T11534 T11533 compound replication,forks
R7706 T11535 T11527 aux would,require
R7707 T11536 T11527 dobj more,require
R7708 T11537 T11536 prep than,more
R7709 T11538 T11539 nmod Mcm4,complex
R771 T1275 T1272 punct ", ",activation
R7710 T11539 T11537 pobj complex,than
R7711 T11540 T11538 punct /,Mcm4
R7712 T11541 T11538 nummod 6,Mcm4
R7713 T11542 T11538 punct /,Mcm4
R7714 T11543 T11538 nummod 7,Mcm4
R7715 T11544 T11509 punct .,indicates
R7716 T11546 T11547 prep During,stimulated
R7717 T11548 T11549 det the,unwinding
R7718 T11549 T11546 pobj unwinding,During
R7719 T11550 T11549 amod processive,unwinding
R772 T1276 T1272 conj mode,activation
R7720 T11551 T11549 prep of,unwinding
R7721 T11552 T11553 compound duplex,forks
R7722 T11553 T11551 pobj forks,of
R7723 T11554 T11553 compound DNA,forks
R7724 T11555 T11553 compound replication,forks
R7725 T11556 T11547 punct ", ",stimulated
R7726 T11557 T11547 nsubjpass Mcm4,stimulated
R7727 T11558 T11557 punct /,Mcm4
R7728 T11559 T11557 nummod 6,Mcm4
R7729 T11560 T11557 punct /,Mcm4
R773 T1277 T1276 prep of,mode
R7730 T11561 T11557 nummod 7,Mcm4
R7731 T11562 T11547 aux may,stimulated
R7732 T11563 T11547 auxpass be,stimulated
R7733 T11564 T11547 advmod further,stimulated
R7734 T11565 T11547 agent by,stimulated
R7735 T11566 T11565 pobj interaction,by
R7736 T11567 T11566 prep with,interaction
R7737 T11568 T11569 amod other,proteins
R7738 T11569 T11567 pobj proteins,with
R7739 T11570 T11569 punct ", ",proteins
R774 T1278 T1277 pobj interaction,of
R7740 T11571 T11569 prep including,proteins
R7741 T11572 T11573 amod remaining,subunits
R7742 T11573 T11571 pobj subunits,including
R7743 T11574 T11573 compound Mcm,subunits
R7744 T11575 T11573 punct ", ",subunits
R7745 T11576 T11573 conj Cdc45,subunits
R7746 T11577 T11578 punct (,38
R7747 T11578 T11576 parataxis 38,Cdc45
R7748 T11579 T11578 nummod 37,38
R7749 T11580 T11578 punct ",",38
R775 T1279 T1278 prep with,interaction
R7750 T11581 T11578 punct ),38
R7751 T11582 T11576 punct ", ",Cdc45
R7752 T11583 T11576 conj GINS,Cdc45
R7753 T11584 T11585 punct (,42
R7754 T11585 T11583 parataxis 42,GINS
R7755 T11586 T11585 nummod 41,42
R7756 T11587 T11585 punct ",",42
R7757 T11588 T11585 punct ),42
R7758 T11589 T11583 punct ", ",GINS
R7759 T11590 T11591 compound DNA,subunits
R776 T1280 T1281 compound DNA,substrates
R7760 T11591 T11583 conj subunits,GINS
R7761 T11592 T11591 compound polymerase,subunits
R7762 T11593 T11594 punct (,43
R7763 T11594 T11591 parataxis 43,subunits
R7764 T11595 T11594 punct ),43
R7765 T11596 T11591 punct ", ",subunits
R7766 T11597 T11591 conj Mcm8,subunits
R7767 T11598 T11599 punct (,36
R7768 T11599 T11597 parataxis 36,Mcm8
R7769 T11600 T11599 nummod 35,36
R777 T1281 T1279 pobj substrates,with
R7770 T11601 T11599 punct ",",36
R7771 T11602 T11599 punct ),36
R7772 T11603 T11597 punct ", ",Mcm8
R7773 T11604 T11597 conj etc.,Mcm8
R7774 T11605 T11606 aux to,become
R7775 T11606 T11547 advcl become,stimulated
R7776 T11607 T11608 det a,helicase
R7777 T11608 T11606 attr helicase,become
R7778 T11609 T11610 advmod truly,processive
R7779 T11610 T11608 amod processive,helicase
R778 T1282 T1276 cc and,mode
R7780 T11611 T11610 cc and,processive
R7781 T11612 T11610 conj potent,processive
R7782 T11613 T11608 amod capable,helicase
R7783 T11614 T11613 prep of,capable
R7784 T11615 T11614 pcomp replicating,of
R7785 T11616 T11617 det the,genome
R7786 T11617 T11615 dobj genome,replicating
R7787 T11618 T11617 amod entire,genome
R7788 T11619 T11547 punct .,stimulated
R779 T1283 T1284 mark whether,required
R780 T1284 T1276 conj required,mode
R781 T1285 T1286 det the,sequences
R782 T1286 T1284 nsubjpass sequences,required
R783 T1287 T1286 compound thymine,sequences
R784 T1288 T1284 auxpass are,required
R785 T1289 T1284 prep for,required
R786 T1290 T1291 amod continuous,activation
R787 T1291 T1289 pobj activation,for
R788 T1292 T1291 prep of,activation
R789 T1293 T1294 compound Mcm,helicase
R790 T1294 T1292 pobj helicase,of
R791 T1295 T1284 prep during,required
R792 T1296 T1297 det the,process
R793 T1297 T1295 pobj process,during
R794 T1298 T1297 amod unwinding,process
R795 T1299 T1266 punct .,addressed
R796 T1301 T1302 det The,results
R797 T1302 T1303 nsubj results,indicate
R798 T1304 T1305 mark that,binds
R799 T1305 T1303 advcl binds,indicate
R800 T1306 T1307 amod mammalian,Mcm4
R801 T1307 T1305 nsubj Mcm4,binds
R802 T1308 T1307 punct /,Mcm4
R803 T1309 T1307 nummod 6,Mcm4
R804 T1310 T1307 punct /,Mcm4
R805 T1311 T1307 nummod 7,Mcm4
R806 T1312 T1305 advmod primarily,binds
R807 T1313 T1305 prep to,binds
R808 T1314 T1315 amod single,stranded
R809 T1315 T1317 amod stranded,region
R810 T1316 T1315 punct -,stranded
R811 T1317 T1313 pobj region,to
R812 T1318 T1317 compound DNA,region
R813 T1319 T1305 punct ", ",binds
R814 T1320 T1305 cc and,binds
R815 T1321 T1322 mark that,related
R816 T1322 T1305 conj related,binds
R817 T1323 T1324 det the,extent
R818 T1324 T1322 nsubjpass extent,related
R819 T1325 T1324 prep of,extent
R820 T1326 T1327 compound helicase,activation
R821 T1327 T1325 pobj activation,of
R822 T1328 T1322 auxpass is,related
R823 T1329 T1322 prep to,related
R824 T1330 T1331 det the,content
R825 T1331 T1329 pobj content,to
R826 T1332 T1331 compound thymine,content
R827 T1333 T1331 prep of,content
R828 T1334 T1335 det the,segment
R829 T1335 T1333 pobj segment,of
R830 T1336 T1337 amod single,stranded
R831 T1337 T1335 amod stranded,segment
R832 T1338 T1337 punct -,stranded
R833 T1339 T1303 punct .,indicate
R834 T1341 T1342 advmod Unexpectedly,is
R835 T1343 T1342 punct ", ",is
R836 T1344 T1345 nmod Mcm4,helicase
R837 T1345 T1342 nsubj helicase,is
R838 T1346 T1344 punct /,Mcm4
R839 T1347 T1344 nummod 6,Mcm4
R840 T1348 T1344 punct /,Mcm4
R841 T1349 T1344 nummod 7,Mcm4
R842 T1350 T1342 neg not,is
R843 T1351 T1342 acomp capable,is
R844 T1352 T1351 prep of,capable
R845 T1353 T1354 advmod efficiently,unwinding
R846 T1354 T1352 pcomp unwinding,of
R847 T1355 T1356 det the,segment
R848 T1356 T1354 dobj segment,unwinding
R849 T1357 T1358 npadvmod GC,rich
R850 T1358 T1356 amod rich,segment
R851 T1359 T1358 punct -,rich
R852 T1360 T1356 compound duplex,segment
R853 T1361 T1342 punct ", ",is
R854 T1362 T1342 advcl suggesting,is
R855 T1363 T1364 mark that,required
R856 T1364 T1362 ccomp required,suggesting
R857 T1365 T1366 det some,mechanism
R858 T1366 T1364 nsubjpass mechanism,required
R859 T1367 T1366 amod other,mechanism
R860 T1368 T1364 aux may,required
R861 T1369 T1364 auxpass be,required
R862 T1370 T1364 prep for,required
R863 T1371 T1370 pobj completion,for
R864 T1372 T1371 prep of,completion
R865 T1373 T1372 pobj replication,of
R866 T1374 T1373 prep of,replication
R867 T1375 T1376 det the,genome
R868 T1376 T1374 pobj genome,of
R869 T1377 T1376 amod entire,genome
R870 T1378 T1342 punct .,is
R872 T1450 T1451 amod Labeled,rNTPs
R873 T1451 T1456 nsubjpass rNTPs,purchased
R874 T1452 T1450 cc and,Labeled
R875 T1453 T1450 conj unlabeled,Labeled
R876 T1454 T1451 compound dNTPs,rNTPs
R877 T1455 T1451 punct /,rNTPs
R878 T1457 T1451 cc and,rNTPs
R879 T1458 T1451 conj Sequenase,rNTPs
R880 T1459 T1456 auxpass were,purchased
R881 T1460 T1456 prep from,purchased
R882 T1461 T1462 compound Amersham,Pharmacia
R883 T1462 T1460 pobj Pharmacia,from
R884 T1463 T1456 punct .,purchased
R885 T1465 T1466 nmod M13mp18,DNA
R886 T1466 T1473 nsubj DNA,were
R887 T1467 T1465 cc and,M13mp18
R888 T1468 T1465 conj M13mp19,M13mp18
R889 T1469 T1470 amod single,stranded
R890 T1470 T1466 amod stranded,DNA
R891 T1471 T1470 punct -,stranded
R892 T1472 T1466 amod circular,DNA
R893 T1474 T1466 punct ", ",DNA
R894 T1475 T1476 compound T4,kinase
R895 T1476 T1466 conj kinase,DNA
R896 T1477 T1476 compound polynucleotide,kinase
R897 T1478 T1476 punct ", ",kinase
R898 T1479 T1476 conj DNase,kinase
R899 T1480 T1479 nummod I,DNase
R900 T1481 T1479 cc and,DNase
R901 T1482 T1483 det the,fragment
R902 T1483 T1479 conj fragment,DNase
R903 T1484 T1483 compound Klenow,fragment
R904 T1485 T1473 prep from,were
R905 T1486 T1485 pobj TAKARA,from
R906 T1487 T1473 punct ", ",were
R907 T1488 T1489 compound Nuclease,P1
R908 T1489 T1490 nsubj P1,was
R909 T1490 T1473 conj was,were
R910 T1491 T1490 prep from,was
R911 T1492 T1491 pobj Roche,from
R912 T1493 T1490 punct ", ",was
R913 T1494 T1490 cc and,was
R914 T1495 T1496 amod anti-FLAG,agarose
R915 T1496 T1503 nsubj agarose,were
R916 T1497 T1496 nmod M2,agarose
R917 T1498 T1499 nmod Ab,antibody
R918 T1499 T1496 nmod antibody,agarose
R919 T1500 T1499 punct (,antibody
R920 T1501 T1496 punct ),agarose
R921 T1502 T1496 punct -,agarose
R922 T1503 T1490 conj were,was
R923 T1504 T1496 cc and,agarose
R924 T1505 T1506 compound FLAG,peptide
R925 T1506 T1496 conj peptide,agarose
R926 T1507 T1503 prep from,were
R927 T1508 T1507 pobj Sigma,from
R928 T1509 T1503 punct .,were
R929 T1511 T1512 compound SV40,antigen
R930 T1512 T1515 nsubjpass antigen,purified
R931 T1513 T1512 compound T,antigen
R932 T1514 T1512 punct -,antigen
R933 T1516 T1515 auxpass was,purified
R934 T1517 T1515 prep from,purified
R935 T1518 T1519 npadvmod baculovirus,infected
R936 T1519 T1521 amod infected,cells
R937 T1520 T1519 punct -,infected
R938 T1521 T1517 pobj cells,from
R939 T1522 T1521 compound insect,cells
R940 T1523 T1524 mark as,reported
R941 T1524 T1515 advcl reported,purified
R942 T1525 T1526 punct (,22
R943 T1526 T1524 parataxis 22,reported
R944 T1527 T1526 punct ),22
R945 T1528 T1515 punct .,purified
R946 T1530 T1531 compound PriA,helicase
R947 T1531 T1532 compound helicase,protein
R948 T1532 T1533 nsubjpass protein,provided
R949 T1534 T1533 auxpass was,provided
R950 T1535 T1533 agent by,provided
R951 T1536 T1537 compound Dr,Tanaka
R952 T1537 T1535 pobj Tanaka,by
R953 T1538 T1537 compound Taku,Tanaka
R954 T1539 T1540 punct (,23
R955 T1540 T1533 parataxis 23,provided
R956 T1541 T1540 punct ),23
R957 T1542 T1533 punct .,provided
R958 T1544 T1545 nsubjpass Oligonucleotides,purchased
R959 T1546 T1545 auxpass were,purchased
R960 T1547 T1545 prep from,purchased
R961 T1548 T1549 nmod Hokkaido,Ltd
R962 T1549 T1547 pobj Ltd,from
R963 T1550 T1549 nmod system,Ltd
R964 T1551 T1549 nmod science,Ltd
R965 T1552 T1549 nmod Co.,Ltd
R966 T1553 T1549 punct ", ",Ltd
R967 T1554 T1555 punct (,Hokkaido
R968 T1555 T1549 parataxis Hokkaido,Ltd
R969 T1556 T1555 punct ", ",Hokkaido
R970 T1557 T1555 npadvmod Japan,Hokkaido
R971 T1558 T1555 punct ),Hokkaido
R972 T1559 T1545 punct ", ",purchased
R973 T1560 T1545 cc and,purchased
R974 T1561 T1562 auxpass were,purified
R975 T1562 T1545 conj purified,purchased
R976 T1563 T1562 prep on,purified
R977 T1564 T1565 compound polyacrylamide,gel
R978 T1565 T1563 pobj gel,on
R979 T1566 T1562 punct ", ",purified
R980 T1567 T1562 advcl followed,purified
R981 T1568 T1567 agent by,followed
R982 T1569 T1568 pobj purification,by
R983 T1570 T1569 prep by,purification
R984 T1571 T1572 compound Sep,Plus
R985 T1572 T1575 compound Plus,cartridge
R986 T1573 T1572 punct -,Plus
R987 T1574 T1572 compound Pak,Plus
R988 T1575 T1577 compound cartridge,column
R989 T1576 T1575 compound C18,cartridge
R990 T1577 T1570 pobj column,by
R991 T1578 T1579 punct (,Waters
R992 T1579 T1577 parataxis Waters,column
R993 T1580 T1579 punct ),Waters
R994 T1581 T1545 punct .,purchased
R995 T1668 T1667 cc and,Expression
R996 T1669 T1667 conj purification,Expression
R997 T1670 T1667 prep of,Expression
R998 T1671 T1672 nmod mouse,complex
R999 T1672 T1670 pobj complex,of