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PMC:1140370 / 13308-15158 JSONTXT 5 Projects

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Id Subject Object Predicate Lexical cue
T4094 0-3 DT denotes The
T4096 4-9 JJ denotes above
T4095 10-17 NNS denotes results
T4097 18-25 VBP denotes suggest
T4098 26-30 IN denotes that
T4100 31-35 NN denotes Mcm4
T4101 35-36 HYPH denotes /
T4102 36-37 CD denotes 6
T4103 37-38 HYPH denotes /
T4104 38-39 CD denotes 7
T4099 40-49 VBZ denotes generates
T4105 50-51 DT denotes a
T4107 52-58 JJ denotes double
T4108 59-68 JJ denotes hexameric
T4106 69-76 NN denotes complex
T4109 77-79 IN denotes on
T4110 80-81 DT denotes a
T4111 82-88 NN denotes bubble
T4112 88-90 , denotes ,
T4113 90-94 DT denotes each
T4114 95-102 NN denotes hexamer
T4116 103-105 IN denotes of
T4117 106-111 WDT denotes which
T4115 112-117 VBZ denotes makes
T4118 118-127 JJ denotes symmetric
T4119 128-135 NN denotes contact
T4120 136-140 IN denotes with
T4121 141-144 DT denotes the
T4123 145-146 CD denotes 5
T4125 146-147 SYM denotes ′
T4126 147-148 HYPH denotes -
T4124 148-156 JJ denotes proximal
T4127 157-164 JJ denotes central
T4128 165-171 JJ denotes single
T4130 171-172 HYPH denotes -
T4129 172-180 VBN denotes stranded
T4122 181-188 NN denotes segment
T4131 188-189 . denotes .
T4132 189-312 sentence denotes This predicts that the single-stranded segment needs to be of a sufficient length in order to accommodate the Mcm complex.
T4133 190-194 DT denotes This
T4134 195-203 VBZ denotes predicts
T4135 204-208 IN denotes that
T4137 209-212 DT denotes the
T4139 213-219 JJ denotes single
T4141 219-220 HYPH denotes -
T4140 220-228 VBN denotes stranded
T4138 229-236 NN denotes segment
T4136 237-242 VBZ denotes needs
T4142 243-245 TO denotes to
T4143 246-248 VB denotes be
T4144 249-251 IN denotes of
T4145 252-253 DT denotes a
T4147 254-264 JJ denotes sufficient
T4146 265-271 NN denotes length
T4148 272-274 IN denotes in
T4149 275-280 NN denotes order
T4150 281-283 TO denotes to
T4151 284-295 VB denotes accommodate
T4152 296-299 DT denotes the
T4154 300-303 NN denotes Mcm
T4153 304-311 NN denotes complex
T4155 311-312 . denotes .
T4156 312-536 sentence denotes In fact, we previously showed that the Mcm4/6/7 mainly forms a single hexameric complex on bubble-20 (a bubble substrate with 20 nt long central single-stranded segment) and a double hexameric complex on bubble-60 DNA (13).
T4157 313-315 IN denotes In
T4159 316-320 NN denotes fact
T4160 320-322 , denotes ,
T4161 322-324 PRP denotes we
T4162 325-335 RB denotes previously
T4158 336-342 VBD denotes showed
T4163 343-347 IN denotes that
T4165 348-351 DT denotes the
T4166 352-356 NN denotes Mcm4
T4167 356-357 HYPH denotes /
T4168 357-358 CD denotes 6
T4169 358-359 HYPH denotes /
T4170 359-360 CD denotes 7
T4171 361-367 RB denotes mainly
T4164 368-373 VBZ denotes forms
T4172 374-375 DT denotes a
T4174 376-382 JJ denotes single
T4175 383-392 JJ denotes hexameric
T4173 393-400 NN denotes complex
T4176 401-403 IN denotes on
T4177 404-410 NN denotes bubble
T4178 410-411 HYPH denotes -
T4179 411-413 CD denotes 20
T4180 414-415 -LRB- denotes (
T4181 415-416 DT denotes a
T4183 417-423 NN denotes bubble
T4182 424-433 NN denotes substrate
T4184 434-438 IN denotes with
T4185 439-441 CD denotes 20
T4186 442-444 NN denotes nt
T4187 445-449 JJ denotes long
T4189 450-457 JJ denotes central
T4190 458-464 JJ denotes single
T4192 464-465 HYPH denotes -
T4191 465-473 VBN denotes stranded
T4188 474-481 NN denotes segment
T4193 481-482 -RRB- denotes )
T4194 483-486 CC denotes and
T4195 487-488 DT denotes a
T4197 489-495 JJ denotes double
T4198 496-505 JJ denotes hexameric
T4196 506-513 NN denotes complex
T4199 514-516 IN denotes on
T4200 517-523 NN denotes bubble
T4202 523-524 HYPH denotes -
T4203 524-526 CD denotes 60
T4201 527-530 NN denotes DNA
T4204 531-532 -LRB- denotes (
T4205 532-534 CD denotes 13
T4206 534-535 -RRB- denotes )
T4207 535-536 . denotes .
T4208 536-868 sentence denotes In order to determine the minimum requirement for double-hexamer formation, a set of synthetic bubble-like substrates (Bub-T10, Bub-T20, Bub-T30, Bub-T40 and Bub-T50) that differed in the length of the central unpaired segment were constructed, and the binding and helicase activities of Mcm4/6/7 on these substrates were examined.
T4209 537-539 IN denotes In
T4211 540-545 NN denotes order
T4212 546-548 TO denotes to
T4213 549-558 VB denotes determine
T4214 559-562 DT denotes the
T4216 563-570 JJ denotes minimum
T4215 571-582 NN denotes requirement
T4217 583-586 IN denotes for
T4218 587-593 JJ denotes double
T4220 593-594 HYPH denotes -
T4219 594-601 NN denotes hexamer
T4221 602-611 NN denotes formation
T4222 611-613 , denotes ,
T4223 613-614 DT denotes a
T4224 615-618 NN denotes set
T4225 619-621 IN denotes of
T4226 622-631 JJ denotes synthetic
T4228 632-638 NN denotes bubble
T4230 638-639 HYPH denotes -
T4229 639-643 JJ denotes like
T4227 644-654 NNS denotes substrates
T4231 655-656 -LRB- denotes (
T4232 656-659 NN denotes Bub
T4234 659-660 HYPH denotes -
T4233 660-663 NN denotes T10
T4235 663-665 , denotes ,
T4236 665-668 NN denotes Bub
T4238 668-669 HYPH denotes -
T4237 669-672 NN denotes T20
T4239 672-674 , denotes ,
T4240 674-677 NN denotes Bub
T4242 677-678 HYPH denotes -
T4241 678-681 NN denotes T30
T4243 681-683 , denotes ,
T4244 683-686 NN denotes Bub
T4246 686-687 HYPH denotes -
T4245 687-690 NN denotes T40
T4247 691-694 CC denotes and
T4248 695-698 NN denotes Bub
T4250 698-699 HYPH denotes -
T4249 699-702 NN denotes T50
T4251 702-703 -RRB- denotes )
T4252 704-708 WDT denotes that
T4253 709-717 VBD denotes differed
T4254 718-720 IN denotes in
T4255 721-724 DT denotes the
T4256 725-731 NN denotes length
T4257 732-734 IN denotes of
T4258 735-738 DT denotes the
T4260 739-746 JJ denotes central
T4261 747-755 JJ denotes unpaired
T4259 756-763 NN denotes segment
T4262 764-768 VBD denotes were
T4210 769-780 VBN denotes constructed
T4263 780-782 , denotes ,
T4264 782-785 CC denotes and
T4265 786-789 DT denotes the
T4267 790-797 NN denotes binding
T4268 798-801 CC denotes and
T4269 802-810 NN denotes helicase
T4266 811-821 NNS denotes activities
T4271 822-824 IN denotes of
T4272 825-829 NN denotes Mcm4
T4273 829-830 HYPH denotes /
T4274 830-831 CD denotes 6
T4275 831-832 HYPH denotes /
T4276 832-833 CD denotes 7
T4277 834-836 IN denotes on
T4278 837-842 DT denotes these
T4279 843-853 NNS denotes substrates
T4280 854-858 VBD denotes were
T4270 859-867 VBN denotes examined
T4281 867-868 . denotes .
T4282 868-1000 sentence denotes Mcm4/6/7 was incubated with a radiolabeled synthetic bubble substrate in the presence of 0.5 mM ATP-γ-S to allow complex formation.
T4283 869-873 NN denotes Mcm4
T4285 873-874 HYPH denotes /
T4286 874-875 CD denotes 6
T4287 875-876 HYPH denotes /
T4288 876-877 CD denotes 7
T4289 878-881 VBD denotes was
T4284 882-891 VBN denotes incubated
T4290 892-896 IN denotes with
T4291 897-898 DT denotes a
T4293 899-911 VBN denotes radiolabeled
T4294 912-921 JJ denotes synthetic
T4295 922-928 NN denotes bubble
T4292 929-938 NN denotes substrate
T4296 939-941 IN denotes in
T4297 942-945 DT denotes the
T4298 946-954 NN denotes presence
T4299 955-957 IN denotes of
T4300 958-961 CD denotes 0.5
T4301 962-964 NN denotes mM
T4303 965-968 NN denotes ATP
T4304 968-969 HYPH denotes -
T4305 969-970 SYM denotes γ
T4306 970-971 HYPH denotes -
T4302 971-972 NN denotes S
T4307 973-975 TO denotes to
T4308 976-981 VB denotes allow
T4309 982-989 NN denotes complex
T4310 990-999 NN denotes formation
T4311 999-1000 . denotes .
T4312 1000-1212 sentence denotes A half of these reaction mixtures were analyzed for DNA binding in gel shift assays (Figure 2A), and the remainder was further incubated in the presence of 10 mM ATP to measure DNA helicase activity (Figure 2B).
T4313 1001-1002 DT denotes A
T4314 1003-1007 NN denotes half
T4316 1008-1010 IN denotes of
T4317 1011-1016 DT denotes these
T4319 1017-1025 NN denotes reaction
T4318 1026-1034 NNS denotes mixtures
T4320 1035-1039 VBD denotes were
T4315 1040-1048 VBN denotes analyzed
T4321 1049-1052 IN denotes for
T4322 1053-1056 NN denotes DNA
T4323 1057-1064 NN denotes binding
T4324 1065-1067 IN denotes in
T4325 1068-1071 NN denotes gel
T4326 1072-1077 NN denotes shift
T4327 1078-1084 NNS denotes assays
T4328 1085-1086 -LRB- denotes (
T4330 1086-1092 NN denotes Figure
T4329 1093-1095 NN denotes 2A
T4331 1095-1096 -RRB- denotes )
T4332 1096-1098 , denotes ,
T4333 1098-1101 CC denotes and
T4334 1102-1105 DT denotes the
T4335 1106-1115 NN denotes remainder
T4337 1116-1119 VBD denotes was
T4338 1120-1127 RB denotes further
T4336 1128-1137 VBN denotes incubated
T4339 1138-1140 IN denotes in
T4340 1141-1144 DT denotes the
T4341 1145-1153 NN denotes presence
T4342 1154-1156 IN denotes of
T4343 1157-1159 CD denotes 10
T4344 1160-1162 NN denotes mM
T4345 1163-1166 NN denotes ATP
T4346 1167-1169 TO denotes to
T4347 1170-1177 VB denotes measure
T4348 1178-1181 NN denotes DNA
T4349 1182-1190 NN denotes helicase
T4350 1191-1199 NN denotes activity
T4351 1200-1201 -LRB- denotes (
T4353 1201-1207 NN denotes Figure
T4352 1208-1210 NN denotes 2B
T4354 1210-1211 -RRB- denotes )
T4355 1211-1212 . denotes .
T4356 1212-1385 sentence denotes Bub-10 generated only a low level of complexes with Mcm4/6/7, and Bub-20 generated primarily a single mobility-shifted form, which may contain a single hexamer of Mcm4/6/7.
T4357 1213-1216 NN denotes Bub
T4359 1216-1217 HYPH denotes -
T4360 1217-1219 CD denotes 10
T4358 1220-1229 VBD denotes generated
T4361 1230-1234 RB denotes only
T4363 1235-1236 DT denotes a
T4364 1237-1240 JJ denotes low
T4362 1241-1246 NN denotes level
T4365 1247-1249 IN denotes of
T4366 1250-1259 NNS denotes complexes
T4367 1260-1264 IN denotes with
T4368 1265-1269 NN denotes Mcm4
T4369 1269-1270 HYPH denotes /
T4370 1270-1271 CD denotes 6
T4371 1271-1272 HYPH denotes /
T4372 1272-1273 CD denotes 7
T4373 1273-1275 , denotes ,
T4374 1275-1278 CC denotes and
T4375 1279-1282 NN denotes Bub
T4377 1282-1283 HYPH denotes -
T4378 1283-1285 CD denotes 20
T4376 1286-1295 VBD denotes generated
T4379 1296-1305 RB denotes primarily
T4380 1306-1307 DT denotes a
T4382 1308-1314 JJ denotes single
T4383 1315-1323 NN denotes mobility
T4385 1323-1324 HYPH denotes -
T4384 1324-1331 VBN denotes shifted
T4381 1332-1336 NN denotes form
T4386 1336-1338 , denotes ,
T4387 1338-1343 WDT denotes which
T4389 1344-1347 MD denotes may
T4388 1348-1355 VB denotes contain
T4390 1356-1357 DT denotes a
T4392 1358-1364 JJ denotes single
T4391 1365-1372 NN denotes hexamer
T4393 1373-1375 IN denotes of
T4394 1376-1380 NN denotes Mcm4
T4395 1380-1381 HYPH denotes /
T4396 1381-1382 CD denotes 6
T4397 1382-1383 HYPH denotes /
T4398 1383-1384 CD denotes 7
T4399 1384-1385 . denotes .
T4400 1385-1541 sentence denotes On Bub-30, significant amount of complexes containing a double hexamer were generated in addition to those containing a single hexamer (Figure 2A) (13,21).
T4401 1386-1388 IN denotes On
T4403 1389-1392 NN denotes Bub
T4404 1392-1393 HYPH denotes -
T4405 1393-1395 CD denotes 30
T4406 1395-1397 , denotes ,
T4407 1397-1408 JJ denotes significant
T4408 1409-1415 NN denotes amount
T4409 1416-1418 IN denotes of
T4410 1419-1428 NNS denotes complexes
T4411 1429-1439 VBG denotes containing
T4412 1440-1441 DT denotes a
T4414 1442-1448 JJ denotes double
T4413 1449-1456 NN denotes hexamer
T4415 1457-1461 VBD denotes were
T4402 1462-1471 VBN denotes generated
T4416 1472-1474 IN denotes in
T4417 1475-1483 NN denotes addition
T4418 1484-1486 IN denotes to
T4419 1487-1492 DT denotes those
T4420 1493-1503 VBG denotes containing
T4421 1504-1505 DT denotes a
T4423 1506-1512 JJ denotes single
T4422 1513-1520 NN denotes hexamer
T4424 1521-1522 -LRB- denotes (
T4426 1522-1528 NN denotes Figure
T4425 1529-1531 NN denotes 2A
T4427 1531-1532 -RRB- denotes )
T4428 1533-1534 -LRB- denotes (
T4430 1534-1536 CD denotes 13
T4431 1536-1537 , denotes ,
T4429 1537-1539 CD denotes 21
T4432 1539-1540 -RRB- denotes )
T4433 1540-1541 . denotes .
T4434 1541-1681 sentence denotes The amounts of double hexamer complexes increased on Bub-T40 and Bub-T50 with concomitant decrease of single hexamer complexes (Figure 2A).
T4435 1542-1545 DT denotes The
T4436 1546-1553 NNS denotes amounts
T4438 1554-1556 IN denotes of
T4439 1557-1563 JJ denotes double
T4440 1564-1571 NN denotes hexamer
T4441 1572-1581 NNS denotes complexes
T4437 1582-1591 VBD denotes increased
T4442 1592-1594 IN denotes on
T4443 1595-1598 NN denotes Bub
T4445 1598-1599 HYPH denotes -
T4444 1599-1602 NN denotes T40
T4446 1603-1606 CC denotes and
T4447 1607-1610 NN denotes Bub
T4449 1610-1611 HYPH denotes -
T4448 1611-1614 NN denotes T50
T4450 1615-1619 IN denotes with
T4451 1620-1631 JJ denotes concomitant
T4452 1632-1640 NN denotes decrease
T4453 1641-1643 IN denotes of
T4454 1644-1650 JJ denotes single
T4455 1651-1658 NN denotes hexamer
T4456 1659-1668 NNS denotes complexes
T4457 1669-1670 -LRB- denotes (
T4459 1670-1676 NN denotes Figure
T4458 1677-1679 NN denotes 2A
T4460 1679-1680 -RRB- denotes )
T4461 1680-1681 . denotes .
T4462 1681-1850 sentence denotes The results indicate that a single-stranded segment of at least 40 nt long is required for efficient loading of the double-hexameric Mcm helicase on a bubble substrate.
T4463 1682-1685 DT denotes The
T4464 1686-1693 NNS denotes results
T4465 1694-1702 VBP denotes indicate
T4466 1703-1707 IN denotes that
T4468 1708-1709 DT denotes a
T4470 1710-1716 JJ denotes single
T4472 1716-1717 HYPH denotes -
T4471 1717-1725 VBN denotes stranded
T4469 1726-1733 NN denotes segment
T4473 1734-1736 IN denotes of
T4474 1737-1739 RB denotes at
T4475 1740-1745 RBS denotes least
T4476 1746-1748 CD denotes 40
T4477 1749-1751 NN denotes nt
T4478 1752-1756 JJ denotes long
T4479 1757-1759 VBZ denotes is
T4467 1760-1768 VBN denotes required
T4480 1769-1772 IN denotes for
T4481 1773-1782 JJ denotes efficient
T4482 1783-1790 NN denotes loading
T4483 1791-1793 IN denotes of
T4484 1794-1797 DT denotes the
T4486 1798-1804 JJ denotes double
T4488 1804-1805 HYPH denotes -
T4487 1805-1814 JJ denotes hexameric
T4489 1815-1818 NN denotes Mcm
T4485 1819-1827 NN denotes helicase
T4490 1828-1830 IN denotes on
T4491 1831-1832 DT denotes a
T4493 1833-1839 NN denotes bubble
T4492 1840-1849 NN denotes substrate
T4494 1849-1850 . denotes .
R2632 T4094 T4095 det The,results
R2633 T4095 T4097 nsubj results,suggest
R2634 T4096 T4095 amod above,results
R2635 T4098 T4099 mark that,generates
R2636 T4099 T4097 ccomp generates,suggest
R2637 T4100 T4099 nsubj Mcm4,generates
R2638 T4101 T4100 punct /,Mcm4
R2639 T4102 T4100 nummod 6,Mcm4
R2640 T4103 T4100 punct /,Mcm4
R2641 T4104 T4100 nummod 7,Mcm4
R2642 T4105 T4106 det a,complex
R2643 T4106 T4099 dobj complex,generates
R2644 T4107 T4108 amod double,hexameric
R2645 T4108 T4106 amod hexameric,complex
R2646 T4109 T4099 prep on,generates
R2647 T4110 T4111 det a,bubble
R2648 T4111 T4109 pobj bubble,on
R2649 T4112 T4099 punct ", ",generates
R2650 T4113 T4114 det each,hexamer
R2651 T4114 T4115 dep hexamer,makes
R2652 T4115 T4099 ccomp makes,generates
R2653 T4116 T4114 prep of,hexamer
R2654 T4117 T4116 pobj which,of
R2655 T4118 T4119 amod symmetric,contact
R2656 T4119 T4115 dobj contact,makes
R2657 T4120 T4119 prep with,contact
R2658 T4121 T4122 det the,segment
R2659 T4122 T4120 pobj segment,with
R2660 T4123 T4124 npadvmod 5,proximal
R2661 T4124 T4122 amod proximal,segment
R2662 T4125 T4123 punct ′,5
R2663 T4126 T4124 punct -,proximal
R2664 T4127 T4122 amod central,segment
R2665 T4128 T4129 amod single,stranded
R2666 T4129 T4122 amod stranded,segment
R2667 T4130 T4129 punct -,stranded
R2668 T4131 T4097 punct .,suggest
R2669 T4133 T4134 nsubj This,predicts
R2670 T4135 T4136 mark that,needs
R2671 T4136 T4134 ccomp needs,predicts
R2672 T4137 T4138 det the,segment
R2673 T4138 T4136 nsubj segment,needs
R2674 T4139 T4140 amod single,stranded
R2675 T4140 T4138 amod stranded,segment
R2676 T4141 T4140 punct -,stranded
R2677 T4142 T4143 aux to,be
R2678 T4143 T4136 xcomp be,needs
R2679 T4144 T4143 prep of,be
R2680 T4145 T4146 det a,length
R2681 T4146 T4144 pobj length,of
R2682 T4147 T4146 amod sufficient,length
R2683 T4148 T4143 prep in,be
R2684 T4149 T4148 pobj order,in
R2685 T4150 T4151 aux to,accommodate
R2686 T4151 T4149 acl accommodate,order
R2687 T4152 T4153 det the,complex
R2688 T4153 T4151 dobj complex,accommodate
R2689 T4154 T4153 compound Mcm,complex
R2690 T4155 T4134 punct .,predicts
R2691 T4157 T4158 prep In,showed
R2692 T4159 T4157 pobj fact,In
R2693 T4160 T4158 punct ", ",showed
R2694 T4161 T4158 nsubj we,showed
R2695 T4162 T4158 advmod previously,showed
R2696 T4163 T4164 mark that,forms
R2697 T4164 T4158 ccomp forms,showed
R2698 T4165 T4166 det the,Mcm4
R2699 T4166 T4164 nsubj Mcm4,forms
R2700 T4167 T4166 punct /,Mcm4
R2701 T4168 T4166 nummod 6,Mcm4
R2702 T4169 T4166 punct /,Mcm4
R2703 T4170 T4166 nummod 7,Mcm4
R2704 T4171 T4164 advmod mainly,forms
R2705 T4172 T4173 det a,complex
R2706 T4173 T4164 dobj complex,forms
R2707 T4174 T4175 amod single,hexameric
R2708 T4175 T4173 amod hexameric,complex
R2709 T4176 T4173 prep on,complex
R2710 T4177 T4176 pobj bubble,on
R2711 T4178 T4177 punct -,bubble
R2712 T4179 T4177 nummod 20,bubble
R2713 T4180 T4177 punct (,bubble
R2714 T4181 T4182 det a,substrate
R2715 T4182 T4177 appos substrate,bubble
R2716 T4183 T4182 compound bubble,substrate
R2717 T4184 T4182 prep with,substrate
R2718 T4185 T4186 nummod 20,nt
R2719 T4186 T4187 npadvmod nt,long
R2720 T4187 T4188 amod long,segment
R2721 T4188 T4184 pobj segment,with
R2722 T4189 T4188 amod central,segment
R2723 T4190 T4191 amod single,stranded
R2724 T4191 T4188 amod stranded,segment
R2725 T4192 T4191 punct -,stranded
R2726 T4193 T4173 punct ),complex
R2727 T4194 T4173 cc and,complex
R2728 T4195 T4196 det a,complex
R2729 T4196 T4173 conj complex,complex
R2730 T4197 T4198 amod double,hexameric
R2731 T4198 T4196 amod hexameric,complex
R2732 T4199 T4196 prep on,complex
R2733 T4200 T4201 nmod bubble,DNA
R2734 T4201 T4199 pobj DNA,on
R2735 T4202 T4200 punct -,bubble
R2736 T4203 T4200 nummod 60,bubble
R2737 T4204 T4205 punct (,13
R2738 T4205 T4196 parataxis 13,complex
R2739 T4206 T4205 punct ),13
R2740 T4207 T4158 punct .,showed
R2741 T4209 T4210 prep In,constructed
R2742 T4211 T4209 pobj order,In
R2743 T4212 T4213 aux to,determine
R2744 T4213 T4211 acl determine,order
R2745 T4214 T4215 det the,requirement
R2746 T4215 T4213 dobj requirement,determine
R2747 T4216 T4215 amod minimum,requirement
R2748 T4217 T4215 prep for,requirement
R2749 T4218 T4219 amod double,hexamer
R2750 T4219 T4221 compound hexamer,formation
R2751 T4220 T4219 punct -,hexamer
R2752 T4221 T4217 pobj formation,for
R2753 T4222 T4210 punct ", ",constructed
R2754 T4223 T4224 det a,set
R2755 T4224 T4210 nsubjpass set,constructed
R2756 T4225 T4224 prep of,set
R2757 T4226 T4227 amod synthetic,substrates
R2758 T4227 T4225 pobj substrates,of
R2759 T4228 T4229 npadvmod bubble,like
R2760 T4229 T4227 amod like,substrates
R2761 T4230 T4229 punct -,like
R2762 T4231 T4227 punct (,substrates
R2763 T4232 T4233 compound Bub,T10
R2764 T4233 T4227 appos T10,substrates
R2765 T4234 T4233 punct -,T10
R2766 T4235 T4233 punct ", ",T10
R2767 T4236 T4237 compound Bub,T20
R2768 T4237 T4233 conj T20,T10
R2769 T4238 T4237 punct -,T20
R2770 T4239 T4237 punct ", ",T20
R2771 T4240 T4241 compound Bub,T30
R2772 T4241 T4237 conj T30,T20
R2773 T4242 T4241 punct -,T30
R2774 T4243 T4241 punct ", ",T30
R2775 T4244 T4245 compound Bub,T40
R2776 T4245 T4241 conj T40,T30
R2777 T4246 T4245 punct -,T40
R2778 T4247 T4245 cc and,T40
R2779 T4248 T4249 compound Bub,T50
R2780 T4249 T4245 conj T50,T40
R2781 T4250 T4249 punct -,T50
R2782 T4251 T4227 punct ),substrates
R2783 T4252 T4253 dep that,differed
R2784 T4253 T4227 relcl differed,substrates
R2785 T4254 T4253 prep in,differed
R2786 T4255 T4256 det the,length
R2787 T4256 T4254 pobj length,in
R2788 T4257 T4256 prep of,length
R2789 T4258 T4259 det the,segment
R2790 T4259 T4257 pobj segment,of
R2791 T4260 T4259 amod central,segment
R2792 T4261 T4259 amod unpaired,segment
R2793 T4262 T4210 auxpass were,constructed
R2794 T4263 T4210 punct ", ",constructed
R2795 T4264 T4210 cc and,constructed
R2796 T4265 T4266 det the,activities
R2797 T4266 T4270 nsubjpass activities,examined
R2798 T4267 T4266 nmod binding,activities
R2799 T4268 T4267 cc and,binding
R2800 T4269 T4267 conj helicase,binding
R2801 T4270 T4210 conj examined,constructed
R2802 T4271 T4266 prep of,activities
R2803 T4272 T4271 pobj Mcm4,of
R2804 T4273 T4272 punct /,Mcm4
R2805 T4274 T4272 nummod 6,Mcm4
R2806 T4275 T4272 punct /,Mcm4
R2807 T4276 T4272 nummod 7,Mcm4
R2808 T4277 T4266 prep on,activities
R2809 T4278 T4279 det these,substrates
R2810 T4279 T4277 pobj substrates,on
R2811 T4280 T4270 auxpass were,examined
R2812 T4281 T4210 punct .,constructed
R2813 T4283 T4284 nsubjpass Mcm4,incubated
R2814 T4285 T4283 punct /,Mcm4
R2815 T4286 T4283 nummod 6,Mcm4
R2816 T4287 T4283 punct /,Mcm4
R2817 T4288 T4283 nummod 7,Mcm4
R2818 T4289 T4284 auxpass was,incubated
R2819 T4290 T4284 prep with,incubated
R2820 T4291 T4292 det a,substrate
R2821 T4292 T4290 pobj substrate,with
R2822 T4293 T4292 amod radiolabeled,substrate
R2823 T4294 T4292 amod synthetic,substrate
R2824 T4295 T4292 compound bubble,substrate
R2825 T4296 T4284 prep in,incubated
R2826 T4297 T4298 det the,presence
R2827 T4298 T4296 pobj presence,in
R2828 T4299 T4298 prep of,presence
R2829 T4300 T4301 nummod 0.5,mM
R2830 T4301 T4302 nmod mM,S
R2831 T4302 T4299 pobj S,of
R2832 T4303 T4302 nmod ATP,S
R2833 T4304 T4302 punct -,S
R2834 T4305 T4302 punct γ,S
R2835 T4306 T4302 punct -,S
R2836 T4307 T4308 aux to,allow
R2837 T4308 T4284 advcl allow,incubated
R2838 T4309 T4310 compound complex,formation
R2839 T4310 T4308 dobj formation,allow
R2840 T4311 T4284 punct .,incubated
R2841 T4313 T4314 det A,half
R2842 T4314 T4315 nsubjpass half,analyzed
R2843 T4316 T4314 prep of,half
R2844 T4317 T4318 det these,mixtures
R2845 T4318 T4316 pobj mixtures,of
R2846 T4319 T4318 compound reaction,mixtures
R2847 T4320 T4315 auxpass were,analyzed
R2848 T4321 T4315 prep for,analyzed
R2849 T4322 T4323 compound DNA,binding
R2850 T4323 T4321 pobj binding,for
R2851 T4324 T4315 prep in,analyzed
R2852 T4325 T4326 compound gel,shift
R2853 T4326 T4327 compound shift,assays
R2854 T4327 T4324 pobj assays,in
R2855 T4328 T4329 punct (,2A
R2856 T4329 T4315 parataxis 2A,analyzed
R2857 T4330 T4329 compound Figure,2A
R2858 T4331 T4329 punct ),2A
R2859 T4332 T4315 punct ", ",analyzed
R2860 T4333 T4315 cc and,analyzed
R2861 T4334 T4335 det the,remainder
R2862 T4335 T4336 nsubjpass remainder,incubated
R2863 T4336 T4315 conj incubated,analyzed
R2864 T4337 T4336 auxpass was,incubated
R2865 T4338 T4336 advmod further,incubated
R2866 T4339 T4336 prep in,incubated
R2867 T4340 T4341 det the,presence
R2868 T4341 T4339 pobj presence,in
R2869 T4342 T4341 prep of,presence
R2870 T4343 T4344 nummod 10,mM
R2871 T4344 T4345 compound mM,ATP
R2872 T4345 T4342 pobj ATP,of
R2873 T4346 T4347 aux to,measure
R2874 T4347 T4336 advcl measure,incubated
R2875 T4348 T4349 compound DNA,helicase
R2876 T4349 T4350 compound helicase,activity
R2877 T4350 T4347 dobj activity,measure
R2878 T4351 T4352 punct (,2B
R2879 T4352 T4347 parataxis 2B,measure
R2880 T4353 T4352 compound Figure,2B
R2881 T4354 T4352 punct ),2B
R2882 T4355 T4336 punct .,incubated
R2883 T4357 T4358 nsubj Bub,generated
R2884 T4359 T4357 punct -,Bub
R2885 T4360 T4357 nummod 10,Bub
R2886 T4361 T4362 advmod only,level
R2887 T4362 T4358 dobj level,generated
R2888 T4363 T4362 det a,level
R2889 T4364 T4362 amod low,level
R2890 T4365 T4362 prep of,level
R2891 T4366 T4365 pobj complexes,of
R2892 T4367 T4366 prep with,complexes
R2893 T4368 T4367 pobj Mcm4,with
R2894 T4369 T4368 punct /,Mcm4
R2895 T4370 T4368 nummod 6,Mcm4
R2896 T4371 T4368 punct /,Mcm4
R2897 T4372 T4368 nummod 7,Mcm4
R2898 T4373 T4358 punct ", ",generated
R2899 T4374 T4358 cc and,generated
R2900 T4375 T4376 nsubj Bub,generated
R2901 T4376 T4358 conj generated,generated
R2902 T4377 T4375 punct -,Bub
R2903 T4378 T4375 nummod 20,Bub
R2904 T4379 T4376 advmod primarily,generated
R2905 T4380 T4381 det a,form
R2906 T4381 T4376 dobj form,generated
R2907 T4382 T4381 amod single,form
R2908 T4383 T4384 npadvmod mobility,shifted
R2909 T4384 T4381 amod shifted,form
R2910 T4385 T4384 punct -,shifted
R2911 T4386 T4381 punct ", ",form
R2912 T4387 T4388 dep which,contain
R2913 T4388 T4381 relcl contain,form
R2914 T4389 T4388 aux may,contain
R2915 T4390 T4391 det a,hexamer
R2916 T4391 T4388 dobj hexamer,contain
R2917 T4392 T4391 amod single,hexamer
R2918 T4393 T4391 prep of,hexamer
R2919 T4394 T4393 pobj Mcm4,of
R2920 T4395 T4394 punct /,Mcm4
R2921 T4396 T4394 nummod 6,Mcm4
R2922 T4397 T4394 punct /,Mcm4
R2923 T4398 T4394 nummod 7,Mcm4
R2924 T4399 T4376 punct .,generated
R2925 T4401 T4402 prep On,generated
R2926 T4403 T4401 pobj Bub,On
R2927 T4404 T4403 punct -,Bub
R2928 T4405 T4403 nummod 30,Bub
R2929 T4406 T4402 punct ", ",generated
R2930 T4407 T4408 amod significant,amount
R2931 T4408 T4402 nsubjpass amount,generated
R2932 T4409 T4408 prep of,amount
R2933 T4410 T4409 pobj complexes,of
R2934 T4411 T4410 acl containing,complexes
R2935 T4412 T4413 det a,hexamer
R2936 T4413 T4411 dobj hexamer,containing
R2937 T4414 T4413 amod double,hexamer
R2938 T4415 T4402 auxpass were,generated
R2939 T4416 T4402 prep in,generated
R2940 T4417 T4416 pobj addition,in
R2941 T4418 T4417 prep to,addition
R2942 T4419 T4418 pobj those,to
R2943 T4420 T4419 acl containing,those
R2944 T4421 T4422 det a,hexamer
R2945 T4422 T4420 dobj hexamer,containing
R2946 T4423 T4422 amod single,hexamer
R2947 T4424 T4425 punct (,2A
R2948 T4425 T4402 parataxis 2A,generated
R2949 T4426 T4425 compound Figure,2A
R2950 T4427 T4425 punct ),2A
R2951 T4428 T4429 punct (,21
R2952 T4429 T4402 parataxis 21,generated
R2953 T4430 T4429 nummod 13,21
R2954 T4431 T4429 punct ",",21
R2955 T4432 T4429 punct ),21
R2956 T4433 T4402 punct .,generated
R2957 T4435 T4436 det The,amounts
R2958 T4436 T4437 nsubj amounts,increased
R2959 T4438 T4436 prep of,amounts
R2960 T4439 T4440 amod double,hexamer
R2961 T4440 T4441 compound hexamer,complexes
R2962 T4441 T4438 pobj complexes,of
R2963 T4442 T4437 prep on,increased
R2964 T4443 T4444 compound Bub,T40
R2965 T4444 T4442 pobj T40,on
R2966 T4445 T4444 punct -,T40
R2967 T4446 T4444 cc and,T40
R2968 T4447 T4448 compound Bub,T50
R2969 T4448 T4444 conj T50,T40
R2970 T4449 T4448 punct -,T50
R2971 T4450 T4437 prep with,increased
R2972 T4451 T4452 amod concomitant,decrease
R2973 T4452 T4450 pobj decrease,with
R2974 T4453 T4452 prep of,decrease
R2975 T4454 T4455 amod single,hexamer
R2976 T4455 T4456 compound hexamer,complexes
R2977 T4456 T4453 pobj complexes,of
R2978 T4457 T4458 punct (,2A
R2979 T4458 T4437 parataxis 2A,increased
R2980 T4459 T4458 compound Figure,2A
R2981 T4460 T4458 punct ),2A
R2982 T4461 T4437 punct .,increased
R2983 T4463 T4464 det The,results
R2984 T4464 T4465 nsubj results,indicate
R2985 T4466 T4467 mark that,required
R2986 T4467 T4465 ccomp required,indicate
R2987 T4468 T4469 det a,segment
R2988 T4469 T4467 nsubjpass segment,required
R2989 T4470 T4471 amod single,stranded
R2990 T4471 T4469 amod stranded,segment
R2991 T4472 T4471 punct -,stranded
R2992 T4473 T4469 prep of,segment
R2993 T4474 T4475 advmod at,least
R2994 T4475 T4476 advmod least,40
R2995 T4476 T4477 nummod 40,nt
R2996 T4477 T4473 pobj nt,of
R2997 T4478 T4477 amod long,nt
R2998 T4479 T4467 auxpass is,required
R2999 T4480 T4467 prep for,required
R3000 T4481 T4482 amod efficient,loading
R3001 T4482 T4480 pobj loading,for
R3002 T4483 T4482 prep of,loading
R3003 T4484 T4485 det the,helicase
R3004 T4485 T4483 pobj helicase,of
R3005 T4486 T4487 amod double,hexameric
R3006 T4487 T4485 amod hexameric,helicase
R3007 T4488 T4487 punct -,hexameric
R3008 T4489 T4485 compound Mcm,helicase
R3009 T4490 T4482 prep on,loading
R3010 T4491 T4492 det a,substrate
R3011 T4492 T4490 pobj substrate,on
R3012 T4493 T4492 compound bubble,substrate
R3013 T4494 T4465 punct .,indicate