PMC:1140370 / 10895-28058 JSON TXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T3428	9-13	NN	denotes	Mcm4
T3430	13-14	HYPH	denotes	/
T3431	14-15	CD	denotes	6
T3432	15-16	HYPH	denotes	/
T3433	16-17	CD	denotes	7
T3429	18-25	NN	denotes	complex
T3435	26-35	RB	denotes	primarily
T3434	36-45	VBZ	denotes	interacts
T3436	46-50	IN	denotes	with
T3437	51-54	DT	denotes	the
T3439	55-61	JJ	denotes	single
T3441	61-62	HYPH	denotes	-
T3440	62-70	VBN	denotes	stranded
T3442	71-74	NN	denotes	DNA
T3438	75-82	NN	denotes	segment
T3443	82-230	sentence	denotes	Mcm4/6/7 binds to ‘bubble’ DNA substrates and unwinds the duplex segments when the central single-stranded segment contains thymine stretches (13).
T3444	83-87	NN	denotes	Mcm4
T3446	87-88	HYPH	denotes	/
T3447	88-89	CD	denotes	6
T3448	89-90	HYPH	denotes	/
T3449	90-91	CD	denotes	7
T3445	92-97	VBZ	denotes	binds
T3450	98-100	IN	denotes	to
T3451	101-102	``	denotes	‘
T3453	102-108	NN	denotes	bubble
T3454	108-109	''	denotes	’
T3455	110-113	NN	denotes	DNA
T3452	114-124	NNS	denotes	substrates
T3456	125-128	CC	denotes	and
T3457	129-136	VBZ	denotes	unwinds
T3458	137-140	DT	denotes	the
T3460	141-147	NN	denotes	duplex
T3459	148-156	NNS	denotes	segments
T3461	157-161	WRB	denotes	when
T3463	162-165	DT	denotes	the
T3465	166-173	JJ	denotes	central
T3466	174-180	JJ	denotes	single
T3468	180-181	HYPH	denotes	-
T3467	181-189	VBN	denotes	stranded
T3464	190-197	NN	denotes	segment
T3462	198-206	VBZ	denotes	contains
T3469	207-214	NN	denotes	thymine
T3470	215-224	NNS	denotes	stretches
T3471	225-226	-LRB-	denotes	(
T3472	226-228	CD	denotes	13
T3473	228-229	-RRB-	denotes	)
T3474	229-230	.	denotes	.
T3475	230-489	sentence	denotes	In order to examine the mode of binding of Mcm4/6/7 on bubble DNAs, we conducted nuclease protection assays using labeled bubble substrate DNAs and Mcm4/6/7 in the presence of ATP-γ-S which permits binding to substrate DNA but does not mobilize the helicase.
T3476	231-233	IN	denotes	In
T3478	234-239	NN	denotes	order
T3479	240-242	TO	denotes	to
T3480	243-250	VB	denotes	examine
T3481	251-254	DT	denotes	the
T3482	255-259	NN	denotes	mode
T3483	260-262	IN	denotes	of
T3484	263-270	NN	denotes	binding
T3485	271-273	IN	denotes	of
T3486	274-278	NN	denotes	Mcm4
T3487	278-279	HYPH	denotes	/
T3488	279-280	CD	denotes	6
T3489	280-281	HYPH	denotes	/
T3490	281-282	CD	denotes	7
T3491	283-285	IN	denotes	on
T3492	286-292	NN	denotes	bubble
T3493	293-297	NNS	denotes	DNAs
T3494	297-299	,	denotes	,
T3495	299-301	PRP	denotes	we
T3477	302-311	VBD	denotes	conducted
T3496	312-320	NN	denotes	nuclease
T3498	321-331	NN	denotes	protection
T3497	332-338	NNS	denotes	assays
T3499	339-344	VBG	denotes	using
T3500	345-352	VBN	denotes	labeled
T3502	353-359	NN	denotes	bubble
T3503	360-369	NN	denotes	substrate
T3501	370-374	NNS	denotes	DNAs
T3504	375-378	CC	denotes	and
T3505	379-383	NN	denotes	Mcm4
T3506	383-384	HYPH	denotes	/
T3507	384-385	CD	denotes	6
T3508	385-386	HYPH	denotes	/
T3509	386-387	CD	denotes	7
T3510	388-390	IN	denotes	in
T3511	391-394	DT	denotes	the
T3512	395-403	NN	denotes	presence
T3513	404-406	IN	denotes	of
T3514	407-410	NN	denotes	ATP
T3516	410-411	HYPH	denotes	-
T3517	411-412	SYM	denotes	γ
T3518	412-413	HYPH	denotes	-
T3515	413-414	NN	denotes	S
T3519	415-420	WDT	denotes	which
T3520	421-428	VBZ	denotes	permits
T3521	429-436	NN	denotes	binding
T3522	437-439	IN	denotes	to
T3523	440-449	NN	denotes	substrate
T3524	450-453	NN	denotes	DNA
T3525	454-457	CC	denotes	but
T3526	458-462	VBZ	denotes	does
T3528	463-466	RB	denotes	not
T3527	467-475	VB	denotes	mobilize
T3529	476-479	DT	denotes	the
T3530	480-488	NN	denotes	helicase
T3531	488-489	.	denotes	.
T3532	489-760	sentence	denotes	At an optimum concentration of DNase I, strong cleavages were detected on the 21 bp duplex regions at both ends of the substrates, whereas weaker cleavages were detected on the central single-stranded region, consistent with preference of DNase I to double-stranded DNA.
T3533	490-492	IN	denotes	At
T3535	493-495	DT	denotes	an
T3537	496-503	JJ	denotes	optimum
T3536	504-517	NN	denotes	concentration
T3538	518-520	IN	denotes	of
T3539	521-526	NN	denotes	DNase
T3540	527-528	CD	denotes	I
T3541	528-530	,	denotes	,
T3542	530-536	JJ	denotes	strong
T3543	537-546	NNS	denotes	cleavages
T3544	547-551	VBD	denotes	were
T3534	552-560	VBN	denotes	detected
T3545	561-563	IN	denotes	on
T3546	564-567	DT	denotes	the
T3548	568-570	CD	denotes	21
T3549	571-573	NN	denotes	bp
T3550	574-580	NN	denotes	duplex
T3547	581-588	NNS	denotes	regions
T3551	589-591	IN	denotes	at
T3552	592-596	DT	denotes	both
T3553	597-601	NNS	denotes	ends
T3554	602-604	IN	denotes	of
T3555	605-608	DT	denotes	the
T3556	609-619	NNS	denotes	substrates
T3557	619-621	,	denotes	,
T3558	621-628	IN	denotes	whereas
T3560	629-635	JJR	denotes	weaker
T3561	636-645	NNS	denotes	cleavages
T3562	646-650	VBD	denotes	were
T3559	651-659	VBN	denotes	detected
T3563	660-662	IN	denotes	on
T3564	663-666	DT	denotes	the
T3566	667-674	JJ	denotes	central
T3567	675-681	JJ	denotes	single
T3569	681-682	HYPH	denotes	-
T3568	682-690	VBN	denotes	stranded
T3565	691-697	NN	denotes	region
T3570	697-699	,	denotes	,
T3571	699-709	JJ	denotes	consistent
T3572	710-714	IN	denotes	with
T3573	715-725	NN	denotes	preference
T3574	726-728	IN	denotes	of
T3575	729-734	NN	denotes	DNase
T3576	735-736	CD	denotes	I
T3577	737-739	IN	denotes	to
T3578	740-746	JJ	denotes	double
T3580	746-747	HYPH	denotes	-
T3579	747-755	VBN	denotes	stranded
T3581	756-759	NN	denotes	DNA
T3582	759-760	.	denotes	.
T3583	760-1018	sentence	denotes	With the Bub66/T-rich substrate, strong protection was detected on the entire single-stranded regions of both top and bottom strands with increasing concentration of Mcm4/6/7, consistent with high affinity of Mcm4/6/7 to T-rich bubble sequences (Figure 1A).
T3584	761-765	IN	denotes	With
T3586	766-769	DT	denotes	the
T3588	770-775	NN	denotes	Bub66
T3589	775-776	HYPH	denotes	/
T3590	776-777	NN	denotes	T
T3591	777-778	HYPH	denotes	-
T3592	778-782	JJ	denotes	rich
T3587	783-792	NN	denotes	substrate
T3593	792-794	,	denotes	,
T3594	794-800	JJ	denotes	strong
T3595	801-811	NN	denotes	protection
T3596	812-815	VBD	denotes	was
T3585	816-824	VBN	denotes	detected
T3597	825-827	IN	denotes	on
T3598	828-831	DT	denotes	the
T3600	832-838	JJ	denotes	entire
T3601	839-845	JJ	denotes	single
T3603	845-846	HYPH	denotes	-
T3602	846-854	VBN	denotes	stranded
T3599	855-862	NNS	denotes	regions
T3604	863-865	IN	denotes	of
T3605	866-870	CC	denotes	both
T3606	871-874	JJ	denotes	top
T3608	875-878	CC	denotes	and
T3609	879-885	JJ	denotes	bottom
T3607	886-893	NNS	denotes	strands
T3610	894-898	IN	denotes	with
T3611	899-909	VBG	denotes	increasing
T3612	910-923	NN	denotes	concentration
T3613	924-926	IN	denotes	of
T3614	927-931	NN	denotes	Mcm4
T3615	931-932	HYPH	denotes	/
T3616	932-933	CD	denotes	6
T3617	933-934	HYPH	denotes	/
T3618	934-935	CD	denotes	7
T3619	935-937	,	denotes	,
T3620	937-947	JJ	denotes	consistent
T3621	948-952	IN	denotes	with
T3622	953-957	JJ	denotes	high
T3623	958-966	NN	denotes	affinity
T3624	967-969	IN	denotes	of
T3625	970-974	NN	denotes	Mcm4
T3626	974-975	HYPH	denotes	/
T3627	975-976	CD	denotes	6
T3628	976-977	HYPH	denotes	/
T3629	977-978	CD	denotes	7
T3630	979-981	IN	denotes	to
T3631	982-983	NN	denotes	T
T3633	983-984	HYPH	denotes	-
T3634	984-988	JJ	denotes	rich
T3635	989-995	NN	denotes	bubble
T3632	996-1005	NNS	denotes	sequences
T3636	1006-1007	-LRB-	denotes	(
T3638	1007-1013	NN	denotes	Figure
T3637	1014-1016	NN	denotes	1A
T3639	1016-1017	-RRB-	denotes	)
T3640	1017-1018	.	denotes	.
T3641	1018-1180	sentence	denotes	Similar, but less significant protection was detected on the single-stranded DNA segment with P1 nucleases (Figure 1A), which is specific to single-stranded DNA.
T3642	1019-1026	JJ	denotes	Similar
T3644	1026-1028	,	denotes	,
T3645	1028-1031	CC	denotes	but
T3646	1032-1036	RBR	denotes	less
T3647	1037-1048	JJ	denotes	significant
T3643	1049-1059	NN	denotes	protection
T3649	1060-1063	VBD	denotes	was
T3648	1064-1072	VBN	denotes	detected
T3650	1073-1075	IN	denotes	on
T3651	1076-1079	DT	denotes	the
T3653	1080-1086	JJ	denotes	single
T3655	1086-1087	HYPH	denotes	-
T3654	1087-1095	VBN	denotes	stranded
T3656	1096-1099	NN	denotes	DNA
T3652	1100-1107	NN	denotes	segment
T3657	1108-1112	IN	denotes	with
T3658	1113-1115	NN	denotes	P1
T3659	1116-1125	NNS	denotes	nucleases
T3660	1126-1127	-LRB-	denotes	(
T3662	1127-1133	NN	denotes	Figure
T3661	1134-1136	NN	denotes	1A
T3663	1136-1137	-RRB-	denotes	)
T3664	1137-1139	,	denotes	,
T3665	1139-1144	WDT	denotes	which
T3666	1145-1147	VBZ	denotes	is
T3667	1148-1156	JJ	denotes	specific
T3668	1157-1159	IN	denotes	to
T3669	1160-1166	JJ	denotes	single
T3671	1166-1167	HYPH	denotes	-
T3670	1167-1175	VBN	denotes	stranded
T3672	1176-1179	NN	denotes	DNA
T3673	1179-1180	.	denotes	.
T3674	1180-1311	sentence	denotes	The results indicate that the Mcm complex is loaded onto bubble DNA through binding to both strands of the single-stranded region.
T3675	1181-1184	DT	denotes	The
T3676	1185-1192	NNS	denotes	results
T3677	1193-1201	VBP	denotes	indicate
T3678	1202-1206	IN	denotes	that
T3680	1207-1210	DT	denotes	the
T3682	1211-1214	NN	denotes	Mcm
T3681	1215-1222	NN	denotes	complex
T3683	1223-1225	VBZ	denotes	is
T3679	1226-1232	VBN	denotes	loaded
T3684	1233-1237	IN	denotes	onto
T3685	1238-1244	NN	denotes	bubble
T3686	1245-1248	NN	denotes	DNA
T3687	1249-1256	IN	denotes	through
T3688	1257-1264	NN	denotes	binding
T3689	1265-1267	IN	denotes	to
T3690	1268-1272	DT	denotes	both
T3691	1273-1280	NNS	denotes	strands
T3692	1281-1283	IN	denotes	of
T3693	1284-1287	DT	denotes	the
T3695	1288-1294	JJ	denotes	single
T3697	1294-1295	HYPH	denotes	-
T3696	1295-1303	VBN	denotes	stranded
T3694	1304-1310	NN	denotes	region
T3698	1310-1311	.	denotes	.
T3699	1311-1585	sentence	denotes	Careful examination of Mcm4/6/7 footprints on a bubble indicated strong protection on the 5′-half of the top strand and moderate protection on the remaining 3′-half, as well as on several base-pair duplex segment adjacent to the branch point (Figure 1A and data not shown).
T3700	1312-1319	JJ	denotes	Careful
T3701	1320-1331	NN	denotes	examination
T3703	1332-1334	IN	denotes	of
T3704	1335-1339	NN	denotes	Mcm4
T3706	1339-1340	HYPH	denotes	/
T3707	1340-1341	CD	denotes	6
T3708	1341-1342	HYPH	denotes	/
T3709	1342-1343	CD	denotes	7
T3705	1344-1354	NNS	denotes	footprints
T3710	1355-1357	IN	denotes	on
T3711	1358-1359	DT	denotes	a
T3712	1360-1366	NN	denotes	bubble
T3702	1367-1376	VBD	denotes	indicated
T3713	1377-1383	JJ	denotes	strong
T3714	1384-1394	NN	denotes	protection
T3715	1395-1397	IN	denotes	on
T3716	1398-1401	DT	denotes	the
T3718	1402-1403	CD	denotes	5
T3719	1403-1404	SYM	denotes	′
T3720	1404-1405	HYPH	denotes	-
T3717	1405-1409	NN	denotes	half
T3721	1410-1412	IN	denotes	of
T3722	1413-1416	DT	denotes	the
T3724	1417-1420	JJ	denotes	top
T3723	1421-1427	NN	denotes	strand
T3725	1428-1431	CC	denotes	and
T3726	1432-1440	JJ	denotes	moderate
T3727	1441-1451	NN	denotes	protection
T3728	1452-1454	IN	denotes	on
T3729	1455-1458	DT	denotes	the
T3731	1459-1468	VBG	denotes	remaining
T3732	1469-1470	CD	denotes	3
T3733	1470-1471	SYM	denotes	′
T3734	1471-1472	HYPH	denotes	-
T3730	1472-1476	NN	denotes	half
T3735	1476-1478	,	denotes	,
T3736	1478-1480	RB	denotes	as
T3738	1481-1485	RB	denotes	well
T3737	1486-1488	IN	denotes	as
T3739	1489-1491	IN	denotes	on
T3740	1492-1499	JJ	denotes	several
T3742	1500-1504	NN	denotes	base
T3744	1504-1505	HYPH	denotes	-
T3743	1505-1509	NN	denotes	pair
T3745	1510-1516	NN	denotes	duplex
T3741	1517-1524	NN	denotes	segment
T3746	1525-1533	JJ	denotes	adjacent
T3747	1534-1536	IN	denotes	to
T3748	1537-1540	DT	denotes	the
T3750	1541-1547	NN	denotes	branch
T3749	1548-1553	NN	denotes	point
T3751	1554-1555	-LRB-	denotes	(
T3753	1555-1561	NN	denotes	Figure
T3752	1562-1564	NN	denotes	1A
T3754	1565-1568	CC	denotes	and
T3755	1569-1573	NNS	denotes	data
T3756	1574-1577	RB	denotes	not
T3757	1578-1583	VBN	denotes	shown
T3758	1583-1584	-RRB-	denotes	)
T3759	1584-1585	.	denotes	.
T3760	1585-1657	sentence	denotes	A similar pattern of protection was observed also on the bottom strand.
T3761	1586-1587	DT	denotes	A
T3763	1588-1595	JJ	denotes	similar
T3762	1596-1603	NN	denotes	pattern
T3765	1604-1606	IN	denotes	of
T3766	1607-1617	NN	denotes	protection
T3767	1618-1621	VBD	denotes	was
T3764	1622-1630	VBN	denotes	observed
T3768	1631-1635	RB	denotes	also
T3769	1636-1638	IN	denotes	on
T3770	1639-1642	DT	denotes	the
T3772	1643-1649	JJ	denotes	bottom
T3771	1650-1656	NN	denotes	strand
T3773	1656-1657	.	denotes	.
T3774	1657-1850	sentence	denotes	This suggests that Mcm4/6/7 may bind to a replication bubble substrate with a 2-fold symmetry as a double hexamer (Figure 1A, see the lower scheme), in a manner similar to SV40 T-antigen (21).
T3775	1658-1662	DT	denotes	This
T3776	1663-1671	VBZ	denotes	suggests
T3777	1672-1676	IN	denotes	that
T3779	1677-1681	NN	denotes	Mcm4
T3780	1681-1682	HYPH	denotes	/
T3781	1682-1683	CD	denotes	6
T3782	1683-1684	HYPH	denotes	/
T3783	1684-1685	CD	denotes	7
T3784	1686-1689	MD	denotes	may
T3778	1690-1694	VB	denotes	bind
T3785	1695-1697	IN	denotes	to
T3786	1698-1699	DT	denotes	a
T3788	1700-1711	NN	denotes	replication
T3789	1712-1718	NN	denotes	bubble
T3787	1719-1728	NN	denotes	substrate
T3790	1729-1733	IN	denotes	with
T3791	1734-1735	DT	denotes	a
T3793	1736-1742	CD	denotes	2-fold
T3792	1743-1751	NN	denotes	symmetry
T3794	1752-1754	IN	denotes	as
T3795	1755-1756	DT	denotes	a
T3797	1757-1763	JJ	denotes	double
T3796	1764-1771	NN	denotes	hexamer
T3798	1772-1773	-LRB-	denotes	(
T3800	1773-1779	NN	denotes	Figure
T3799	1780-1782	NN	denotes	1A
T3801	1782-1784	,	denotes	,
T3802	1784-1787	VBP	denotes	see
T3803	1788-1791	DT	denotes	the
T3805	1792-1797	JJ	denotes	lower
T3804	1798-1804	NN	denotes	scheme
T3806	1804-1805	-RRB-	denotes	)
T3807	1805-1807	,	denotes	,
T3808	1807-1809	IN	denotes	in
T3809	1810-1811	DT	denotes	a
T3810	1812-1818	NN	denotes	manner
T3811	1819-1826	JJ	denotes	similar
T3812	1827-1829	IN	denotes	to
T3813	1830-1834	NN	denotes	SV40
T3815	1835-1836	NN	denotes	T
T3816	1836-1837	HYPH	denotes	-
T3814	1837-1844	NN	denotes	antigen
T3817	1845-1846	-LRB-	denotes	(
T3818	1846-1848	CD	denotes	21
T3819	1848-1849	-RRB-	denotes	)
T3820	1849-1850	.	denotes	.
T3821	1850-1941	sentence	denotes	Nuclease protection assays were conducted also on a T-tailed Y-fork structure (Figure 1B).
T3822	1851-1859	NN	denotes	Nuclease
T3823	1860-1870	NN	denotes	protection
T3824	1871-1877	NNS	denotes	assays
T3826	1878-1882	VBD	denotes	were
T3825	1883-1892	VBN	denotes	conducted
T3827	1893-1897	RB	denotes	also
T3828	1898-1900	IN	denotes	on
T3829	1901-1902	DT	denotes	a
T3831	1903-1904	NN	denotes	T
T3833	1904-1905	HYPH	denotes	-
T3832	1905-1911	VBN	denotes	tailed
T3834	1912-1913	NN	denotes	Y
T3836	1913-1914	HYPH	denotes	-
T3835	1914-1918	NN	denotes	fork
T3830	1919-1928	NN	denotes	structure
T3837	1929-1930	-LRB-	denotes	(
T3839	1930-1936	NN	denotes	Figure
T3838	1937-1939	NN	denotes	1B
T3840	1939-1940	-RRB-	denotes	)
T3841	1940-1941	.	denotes	.
T3842	1941-2113	sentence	denotes	Not only the single-stranded tail region but also the 7 bp duplex DNA adjacent to the duplex-to-single-strand junction were protected by Mcm binding from DNaseI digestion.
T3843	1942-1945	RB	denotes	Not
T3845	1946-1950	RB	denotes	only
T3846	1951-1954	DT	denotes	the
T3847	1955-1961	JJ	denotes	single
T3849	1961-1962	HYPH	denotes	-
T3848	1962-1970	VBN	denotes	stranded
T3850	1971-1975	NN	denotes	tail
T3844	1976-1982	NN	denotes	region
T3852	1983-1986	CC	denotes	but
T3853	1987-1991	RB	denotes	also
T3854	1992-1995	DT	denotes	the
T3856	1996-1997	CD	denotes	7
T3857	1998-2000	NN	denotes	bp
T3858	2001-2007	NN	denotes	duplex
T3855	2008-2011	NN	denotes	DNA
T3859	2012-2020	JJ	denotes	adjacent
T3860	2021-2023	IN	denotes	to
T3861	2024-2027	DT	denotes	the
T3863	2028-2034	NN	denotes	duplex
T3864	2034-2035	HYPH	denotes	-
T3865	2035-2037	IN	denotes	to
T3866	2037-2038	HYPH	denotes	-
T3867	2038-2044	JJ	denotes	single
T3869	2044-2045	HYPH	denotes	-
T3868	2045-2051	NN	denotes	strand
T3862	2052-2060	NN	denotes	junction
T3870	2061-2065	VBD	denotes	were
T3851	2066-2075	VBN	denotes	protected
T3871	2076-2078	IN	denotes	by
T3872	2079-2082	NN	denotes	Mcm
T3873	2083-2090	NN	denotes	binding
T3874	2091-2095	IN	denotes	from
T3875	2096-2102	NN	denotes	DNaseI
T3876	2103-2112	NN	denotes	digestion
T3877	2112-2113	.	denotes	.
T3878	2113-2312	sentence	denotes	These results indicate that Mcm4/6/7 protein primarily interacts with single-stranded DNA region and that the interaction also spans the duplex segments near the branchpoint of the replication fork.
T3879	2114-2119	DT	denotes	These
T3880	2120-2127	NNS	denotes	results
T3881	2128-2136	VBP	denotes	indicate
T3882	2137-2141	IN	denotes	that
T3884	2142-2146	NN	denotes	Mcm4
T3886	2146-2147	HYPH	denotes	/
T3887	2147-2148	CD	denotes	6
T3888	2148-2149	HYPH	denotes	/
T3889	2149-2150	CD	denotes	7
T3885	2151-2158	NN	denotes	protein
T3890	2159-2168	RB	denotes	primarily
T3883	2169-2178	VBZ	denotes	interacts
T3891	2179-2183	IN	denotes	with
T3892	2184-2190	JJ	denotes	single
T3894	2190-2191	HYPH	denotes	-
T3893	2191-2199	VBN	denotes	stranded
T3896	2200-2203	NN	denotes	DNA
T3895	2204-2210	NN	denotes	region
T3897	2211-2214	CC	denotes	and
T3898	2215-2219	IN	denotes	that
T3900	2220-2223	DT	denotes	the
T3901	2224-2235	NN	denotes	interaction
T3902	2236-2240	RB	denotes	also
T3899	2241-2246	VBZ	denotes	spans
T3903	2247-2250	DT	denotes	the
T3905	2251-2257	NN	denotes	duplex
T3904	2258-2266	NNS	denotes	segments
T3906	2267-2271	IN	denotes	near
T3907	2272-2275	DT	denotes	the
T3908	2276-2287	NN	denotes	branchpoint
T3909	2288-2290	IN	denotes	of
T3910	2291-2294	DT	denotes	the
T3912	2295-2306	NN	denotes	replication
T3911	2307-2311	NN	denotes	fork
T3913	2311-2312	.	denotes	.
T4077	2314-2323	NN	denotes	Formation
T4079	2324-2326	IN	denotes	of
T4080	2327-2333	JJ	denotes	double
T4081	2334-2343	JJ	denotes	hexameric
T4083	2344-2348	NN	denotes	Mcm4
T4084	2348-2349	HYPH	denotes	/
T4085	2349-2350	CD	denotes	6
T4086	2350-2351	HYPH	denotes	/
T4087	2351-2352	CD	denotes	7
T4082	2353-2362	NNS	denotes	complexes
T4078	2363-2374	VBZ	denotes	facilitates
T4088	2375-2383	NN	denotes	helicase
T4089	2384-2391	NNS	denotes	actions
T4090	2392-2394	IN	denotes	on
T4091	2395-2401	NN	denotes	bubble
T4092	2402-2412	NNS	denotes	substrates
T4093	2412-2602	sentence	denotes	The above results suggest that Mcm4/6/7 generates a double hexameric complex on a bubble, each hexamer of which makes symmetric contact with the 5′-proximal central single-stranded segment.
T4094	2413-2416	DT	denotes	The
T4096	2417-2422	JJ	denotes	above
T4095	2423-2430	NNS	denotes	results
T4097	2431-2438	VBP	denotes	suggest
T4098	2439-2443	IN	denotes	that
T4100	2444-2448	NN	denotes	Mcm4
T4101	2448-2449	HYPH	denotes	/
T4102	2449-2450	CD	denotes	6
T4103	2450-2451	HYPH	denotes	/
T4104	2451-2452	CD	denotes	7
T4099	2453-2462	VBZ	denotes	generates
T4105	2463-2464	DT	denotes	a
T4107	2465-2471	JJ	denotes	double
T4108	2472-2481	JJ	denotes	hexameric
T4106	2482-2489	NN	denotes	complex
T4109	2490-2492	IN	denotes	on
T4110	2493-2494	DT	denotes	a
T4111	2495-2501	NN	denotes	bubble
T4112	2501-2503	,	denotes	,
T4113	2503-2507	DT	denotes	each
T4114	2508-2515	NN	denotes	hexamer
T4116	2516-2518	IN	denotes	of
T4117	2519-2524	WDT	denotes	which
T4115	2525-2530	VBZ	denotes	makes
T4118	2531-2540	JJ	denotes	symmetric
T4119	2541-2548	NN	denotes	contact
T4120	2549-2553	IN	denotes	with
T4121	2554-2557	DT	denotes	the
T4123	2558-2559	CD	denotes	5
T4125	2559-2560	SYM	denotes	′
T4126	2560-2561	HYPH	denotes	-
T4124	2561-2569	JJ	denotes	proximal
T4127	2570-2577	JJ	denotes	central
T4128	2578-2584	JJ	denotes	single
T4130	2584-2585	HYPH	denotes	-
T4129	2585-2593	VBN	denotes	stranded
T4122	2594-2601	NN	denotes	segment
T4131	2601-2602	.	denotes	.
T4132	2602-2725	sentence	denotes	This predicts that the single-stranded segment needs to be of a sufficient length in order to accommodate the Mcm complex.
T4133	2603-2607	DT	denotes	This
T4134	2608-2616	VBZ	denotes	predicts
T4135	2617-2621	IN	denotes	that
T4137	2622-2625	DT	denotes	the
T4139	2626-2632	JJ	denotes	single
T4141	2632-2633	HYPH	denotes	-
T4140	2633-2641	VBN	denotes	stranded
T4138	2642-2649	NN	denotes	segment
T4136	2650-2655	VBZ	denotes	needs
T4142	2656-2658	TO	denotes	to
T4143	2659-2661	VB	denotes	be
T4144	2662-2664	IN	denotes	of
T4145	2665-2666	DT	denotes	a
T4147	2667-2677	JJ	denotes	sufficient
T4146	2678-2684	NN	denotes	length
T4148	2685-2687	IN	denotes	in
T4149	2688-2693	NN	denotes	order
T4150	2694-2696	TO	denotes	to
T4151	2697-2708	VB	denotes	accommodate
T4152	2709-2712	DT	denotes	the
T4154	2713-2716	NN	denotes	Mcm
T4153	2717-2724	NN	denotes	complex
T4155	2724-2725	.	denotes	.
T4156	2725-2949	sentence	denotes	In fact, we previously showed that the Mcm4/6/7 mainly forms a single hexameric complex on bubble-20 (a bubble substrate with 20 nt long central single-stranded segment) and a double hexameric complex on bubble-60 DNA (13).
T4157	2726-2728	IN	denotes	In
T4159	2729-2733	NN	denotes	fact
T4160	2733-2735	,	denotes	,
T4161	2735-2737	PRP	denotes	we
T4162	2738-2748	RB	denotes	previously
T4158	2749-2755	VBD	denotes	showed
T4163	2756-2760	IN	denotes	that
T4165	2761-2764	DT	denotes	the
T4166	2765-2769	NN	denotes	Mcm4
T4167	2769-2770	HYPH	denotes	/
T4168	2770-2771	CD	denotes	6
T4169	2771-2772	HYPH	denotes	/
T4170	2772-2773	CD	denotes	7
T4171	2774-2780	RB	denotes	mainly
T4164	2781-2786	VBZ	denotes	forms
T4172	2787-2788	DT	denotes	a
T4174	2789-2795	JJ	denotes	single
T4175	2796-2805	JJ	denotes	hexameric
T4173	2806-2813	NN	denotes	complex
T4176	2814-2816	IN	denotes	on
T4177	2817-2823	NN	denotes	bubble
T4178	2823-2824	HYPH	denotes	-
T4179	2824-2826	CD	denotes	20
T4180	2827-2828	-LRB-	denotes	(
T4181	2828-2829	DT	denotes	a
T4183	2830-2836	NN	denotes	bubble
T4182	2837-2846	NN	denotes	substrate
T4184	2847-2851	IN	denotes	with
T4185	2852-2854	CD	denotes	20
T4186	2855-2857	NN	denotes	nt
T4187	2858-2862	JJ	denotes	long
T4189	2863-2870	JJ	denotes	central
T4190	2871-2877	JJ	denotes	single
T4192	2877-2878	HYPH	denotes	-
T4191	2878-2886	VBN	denotes	stranded
T4188	2887-2894	NN	denotes	segment
T4193	2894-2895	-RRB-	denotes	)
T4194	2896-2899	CC	denotes	and
T4195	2900-2901	DT	denotes	a
T4197	2902-2908	JJ	denotes	double
T4198	2909-2918	JJ	denotes	hexameric
T4196	2919-2926	NN	denotes	complex
T4199	2927-2929	IN	denotes	on
T4200	2930-2936	NN	denotes	bubble
T4202	2936-2937	HYPH	denotes	-
T4203	2937-2939	CD	denotes	60
T4201	2940-2943	NN	denotes	DNA
T4204	2944-2945	-LRB-	denotes	(
T4205	2945-2947	CD	denotes	13
T4206	2947-2948	-RRB-	denotes	)
T4207	2948-2949	.	denotes	.
T4208	2949-3281	sentence	denotes	In order to determine the minimum requirement for double-hexamer formation, a set of synthetic bubble-like substrates (Bub-T10, Bub-T20, Bub-T30, Bub-T40 and Bub-T50) that differed in the length of the central unpaired segment were constructed, and the binding and helicase activities of Mcm4/6/7 on these substrates were examined.
T4209	2950-2952	IN	denotes	In
T4211	2953-2958	NN	denotes	order
T4212	2959-2961	TO	denotes	to
T4213	2962-2971	VB	denotes	determine
T4214	2972-2975	DT	denotes	the
T4216	2976-2983	JJ	denotes	minimum
T4215	2984-2995	NN	denotes	requirement
T4217	2996-2999	IN	denotes	for
T4218	3000-3006	JJ	denotes	double
T4220	3006-3007	HYPH	denotes	-
T4219	3007-3014	NN	denotes	hexamer
T4221	3015-3024	NN	denotes	formation
T4222	3024-3026	,	denotes	,
T4223	3026-3027	DT	denotes	a
T4224	3028-3031	NN	denotes	set
T4225	3032-3034	IN	denotes	of
T4226	3035-3044	JJ	denotes	synthetic
T4228	3045-3051	NN	denotes	bubble
T4230	3051-3052	HYPH	denotes	-
T4229	3052-3056	JJ	denotes	like
T4227	3057-3067	NNS	denotes	substrates
T4231	3068-3069	-LRB-	denotes	(
T4232	3069-3072	NN	denotes	Bub
T4234	3072-3073	HYPH	denotes	-
T4233	3073-3076	NN	denotes	T10
T4235	3076-3078	,	denotes	,
T4236	3078-3081	NN	denotes	Bub
T4238	3081-3082	HYPH	denotes	-
T4237	3082-3085	NN	denotes	T20
T4239	3085-3087	,	denotes	,
T4240	3087-3090	NN	denotes	Bub
T4242	3090-3091	HYPH	denotes	-
T4241	3091-3094	NN	denotes	T30
T4243	3094-3096	,	denotes	,
T4244	3096-3099	NN	denotes	Bub
T4246	3099-3100	HYPH	denotes	-
T4245	3100-3103	NN	denotes	T40
T4247	3104-3107	CC	denotes	and
T4248	3108-3111	NN	denotes	Bub
T4250	3111-3112	HYPH	denotes	-
T4249	3112-3115	NN	denotes	T50
T4251	3115-3116	-RRB-	denotes	)
T4252	3117-3121	WDT	denotes	that
T4253	3122-3130	VBD	denotes	differed
T4254	3131-3133	IN	denotes	in
T4255	3134-3137	DT	denotes	the
T4256	3138-3144	NN	denotes	length
T4257	3145-3147	IN	denotes	of
T4258	3148-3151	DT	denotes	the
T4260	3152-3159	JJ	denotes	central
T4261	3160-3168	JJ	denotes	unpaired
T4259	3169-3176	NN	denotes	segment
T4262	3177-3181	VBD	denotes	were
T4210	3182-3193	VBN	denotes	constructed
T4263	3193-3195	,	denotes	,
T4264	3195-3198	CC	denotes	and
T4265	3199-3202	DT	denotes	the
T4267	3203-3210	NN	denotes	binding
T4268	3211-3214	CC	denotes	and
T4269	3215-3223	NN	denotes	helicase
T4266	3224-3234	NNS	denotes	activities
T4271	3235-3237	IN	denotes	of
T4272	3238-3242	NN	denotes	Mcm4
T4273	3242-3243	HYPH	denotes	/
T4274	3243-3244	CD	denotes	6
T4275	3244-3245	HYPH	denotes	/
T4276	3245-3246	CD	denotes	7
T4277	3247-3249	IN	denotes	on
T4278	3250-3255	DT	denotes	these
T4279	3256-3266	NNS	denotes	substrates
T4280	3267-3271	VBD	denotes	were
T4270	3272-3280	VBN	denotes	examined
T4281	3280-3281	.	denotes	.
T4282	3281-3413	sentence	denotes	Mcm4/6/7 was incubated with a radiolabeled synthetic bubble substrate in the presence of 0.5 mM ATP-γ-S to allow complex formation.
T4283	3282-3286	NN	denotes	Mcm4
T4285	3286-3287	HYPH	denotes	/
T4286	3287-3288	CD	denotes	6
T4287	3288-3289	HYPH	denotes	/
T4288	3289-3290	CD	denotes	7
T4289	3291-3294	VBD	denotes	was
T4284	3295-3304	VBN	denotes	incubated
T4290	3305-3309	IN	denotes	with
T4291	3310-3311	DT	denotes	a
T4293	3312-3324	VBN	denotes	radiolabeled
T4294	3325-3334	JJ	denotes	synthetic
T4295	3335-3341	NN	denotes	bubble
T4292	3342-3351	NN	denotes	substrate
T4296	3352-3354	IN	denotes	in
T4297	3355-3358	DT	denotes	the
T4298	3359-3367	NN	denotes	presence
T4299	3368-3370	IN	denotes	of
T4300	3371-3374	CD	denotes	0.5
T4301	3375-3377	NN	denotes	mM
T4303	3378-3381	NN	denotes	ATP
T4304	3381-3382	HYPH	denotes	-
T4305	3382-3383	SYM	denotes	γ
T4306	3383-3384	HYPH	denotes	-
T4302	3384-3385	NN	denotes	S
T4307	3386-3388	TO	denotes	to
T4308	3389-3394	VB	denotes	allow
T4309	3395-3402	NN	denotes	complex
T4310	3403-3412	NN	denotes	formation
T4311	3412-3413	.	denotes	.
T4312	3413-3625	sentence	denotes	A half of these reaction mixtures were analyzed for DNA binding in gel shift assays (Figure 2A), and the remainder was further incubated in the presence of 10 mM ATP to measure DNA helicase activity (Figure 2B).
T4313	3414-3415	DT	denotes	A
T4314	3416-3420	NN	denotes	half
T4316	3421-3423	IN	denotes	of
T4317	3424-3429	DT	denotes	these
T4319	3430-3438	NN	denotes	reaction
T4318	3439-3447	NNS	denotes	mixtures
T4320	3448-3452	VBD	denotes	were
T4315	3453-3461	VBN	denotes	analyzed
T4321	3462-3465	IN	denotes	for
T4322	3466-3469	NN	denotes	DNA
T4323	3470-3477	NN	denotes	binding
T4324	3478-3480	IN	denotes	in
T4325	3481-3484	NN	denotes	gel
T4326	3485-3490	NN	denotes	shift
T4327	3491-3497	NNS	denotes	assays
T4328	3498-3499	-LRB-	denotes	(
T4330	3499-3505	NN	denotes	Figure
T4329	3506-3508	NN	denotes	2A
T4331	3508-3509	-RRB-	denotes	)
T4332	3509-3511	,	denotes	,
T4333	3511-3514	CC	denotes	and
T4334	3515-3518	DT	denotes	the
T4335	3519-3528	NN	denotes	remainder
T4337	3529-3532	VBD	denotes	was
T4338	3533-3540	RB	denotes	further
T4336	3541-3550	VBN	denotes	incubated
T4339	3551-3553	IN	denotes	in
T4340	3554-3557	DT	denotes	the
T4341	3558-3566	NN	denotes	presence
T4342	3567-3569	IN	denotes	of
T4343	3570-3572	CD	denotes	10
T4344	3573-3575	NN	denotes	mM
T4345	3576-3579	NN	denotes	ATP
T4346	3580-3582	TO	denotes	to
T4347	3583-3590	VB	denotes	measure
T4348	3591-3594	NN	denotes	DNA
T4349	3595-3603	NN	denotes	helicase
T4350	3604-3612	NN	denotes	activity
T4351	3613-3614	-LRB-	denotes	(
T4353	3614-3620	NN	denotes	Figure
T4352	3621-3623	NN	denotes	2B
T4354	3623-3624	-RRB-	denotes	)
T4355	3624-3625	.	denotes	.
T4356	3625-3798	sentence	denotes	Bub-10 generated only a low level of complexes with Mcm4/6/7, and Bub-20 generated primarily a single mobility-shifted form, which may contain a single hexamer of Mcm4/6/7.
T4357	3626-3629	NN	denotes	Bub
T4359	3629-3630	HYPH	denotes	-
T4360	3630-3632	CD	denotes	10
T4358	3633-3642	VBD	denotes	generated
T4361	3643-3647	RB	denotes	only
T4363	3648-3649	DT	denotes	a
T4364	3650-3653	JJ	denotes	low
T4362	3654-3659	NN	denotes	level
T4365	3660-3662	IN	denotes	of
T4366	3663-3672	NNS	denotes	complexes
T4367	3673-3677	IN	denotes	with
T4368	3678-3682	NN	denotes	Mcm4
T4369	3682-3683	HYPH	denotes	/
T4370	3683-3684	CD	denotes	6
T4371	3684-3685	HYPH	denotes	/
T4372	3685-3686	CD	denotes	7
T4373	3686-3688	,	denotes	,
T4374	3688-3691	CC	denotes	and
T4375	3692-3695	NN	denotes	Bub
T4377	3695-3696	HYPH	denotes	-
T4378	3696-3698	CD	denotes	20
T4376	3699-3708	VBD	denotes	generated
T4379	3709-3718	RB	denotes	primarily
T4380	3719-3720	DT	denotes	a
T4382	3721-3727	JJ	denotes	single
T4383	3728-3736	NN	denotes	mobility
T4385	3736-3737	HYPH	denotes	-
T4384	3737-3744	VBN	denotes	shifted
T4381	3745-3749	NN	denotes	form
T4386	3749-3751	,	denotes	,
T4387	3751-3756	WDT	denotes	which
T4389	3757-3760	MD	denotes	may
T4388	3761-3768	VB	denotes	contain
T4390	3769-3770	DT	denotes	a
T4392	3771-3777	JJ	denotes	single
T4391	3778-3785	NN	denotes	hexamer
T4393	3786-3788	IN	denotes	of
T4394	3789-3793	NN	denotes	Mcm4
T4395	3793-3794	HYPH	denotes	/
T4396	3794-3795	CD	denotes	6
T4397	3795-3796	HYPH	denotes	/
T4398	3796-3797	CD	denotes	7
T4399	3797-3798	.	denotes	.
T4400	3798-3954	sentence	denotes	On Bub-30, significant amount of complexes containing a double hexamer were generated in addition to those containing a single hexamer (Figure 2A) (13,21).
T4401	3799-3801	IN	denotes	On
T4403	3802-3805	NN	denotes	Bub
T4404	3805-3806	HYPH	denotes	-
T4405	3806-3808	CD	denotes	30
T4406	3808-3810	,	denotes	,
T4407	3810-3821	JJ	denotes	significant
T4408	3822-3828	NN	denotes	amount
T4409	3829-3831	IN	denotes	of
T4410	3832-3841	NNS	denotes	complexes
T4411	3842-3852	VBG	denotes	containing
T4412	3853-3854	DT	denotes	a
T4414	3855-3861	JJ	denotes	double
T4413	3862-3869	NN	denotes	hexamer
T4415	3870-3874	VBD	denotes	were
T4402	3875-3884	VBN	denotes	generated
T4416	3885-3887	IN	denotes	in
T4417	3888-3896	NN	denotes	addition
T4418	3897-3899	IN	denotes	to
T4419	3900-3905	DT	denotes	those
T4420	3906-3916	VBG	denotes	containing
T4421	3917-3918	DT	denotes	a
T4423	3919-3925	JJ	denotes	single
T4422	3926-3933	NN	denotes	hexamer
T4424	3934-3935	-LRB-	denotes	(
T4426	3935-3941	NN	denotes	Figure
T4425	3942-3944	NN	denotes	2A
T4427	3944-3945	-RRB-	denotes	)
T4428	3946-3947	-LRB-	denotes	(
T4430	3947-3949	CD	denotes	13
T4431	3949-3950	,	denotes	,
T4429	3950-3952	CD	denotes	21
T4432	3952-3953	-RRB-	denotes	)
T4433	3953-3954	.	denotes	.
T4434	3954-4094	sentence	denotes	The amounts of double hexamer complexes increased on Bub-T40 and Bub-T50 with concomitant decrease of single hexamer complexes (Figure 2A).
T4435	3955-3958	DT	denotes	The
T4436	3959-3966	NNS	denotes	amounts
T4438	3967-3969	IN	denotes	of
T4439	3970-3976	JJ	denotes	double
T4440	3977-3984	NN	denotes	hexamer
T4441	3985-3994	NNS	denotes	complexes
T4437	3995-4004	VBD	denotes	increased
T4442	4005-4007	IN	denotes	on
T4443	4008-4011	NN	denotes	Bub
T4445	4011-4012	HYPH	denotes	-
T4444	4012-4015	NN	denotes	T40
T4446	4016-4019	CC	denotes	and
T4447	4020-4023	NN	denotes	Bub
T4449	4023-4024	HYPH	denotes	-
T4448	4024-4027	NN	denotes	T50
T4450	4028-4032	IN	denotes	with
T4451	4033-4044	JJ	denotes	concomitant
T4452	4045-4053	NN	denotes	decrease
T4453	4054-4056	IN	denotes	of
T4454	4057-4063	JJ	denotes	single
T4455	4064-4071	NN	denotes	hexamer
T4456	4072-4081	NNS	denotes	complexes
T4457	4082-4083	-LRB-	denotes	(
T4459	4083-4089	NN	denotes	Figure
T4458	4090-4092	NN	denotes	2A
T4460	4092-4093	-RRB-	denotes	)
T4461	4093-4094	.	denotes	.
T4462	4094-4263	sentence	denotes	The results indicate that a single-stranded segment of at least 40 nt long is required for efficient loading of the double-hexameric Mcm helicase on a bubble substrate.
T4463	4095-4098	DT	denotes	The
T4464	4099-4106	NNS	denotes	results
T4465	4107-4115	VBP	denotes	indicate
T4466	4116-4120	IN	denotes	that
T4468	4121-4122	DT	denotes	a
T4470	4123-4129	JJ	denotes	single
T4472	4129-4130	HYPH	denotes	-
T4471	4130-4138	VBN	denotes	stranded
T4469	4139-4146	NN	denotes	segment
T4473	4147-4149	IN	denotes	of
T4474	4150-4152	RB	denotes	at
T4475	4153-4158	RBS	denotes	least
T4476	4159-4161	CD	denotes	40
T4477	4162-4164	NN	denotes	nt
T4478	4165-4169	JJ	denotes	long
T4479	4170-4172	VBZ	denotes	is
T4467	4173-4181	VBN	denotes	required
T4480	4182-4185	IN	denotes	for
T4481	4186-4195	JJ	denotes	efficient
T4482	4196-4203	NN	denotes	loading
T4483	4204-4206	IN	denotes	of
T4484	4207-4210	DT	denotes	the
T4486	4211-4217	JJ	denotes	double
T4488	4217-4218	HYPH	denotes	-
T4487	4218-4227	JJ	denotes	hexameric
T4489	4228-4231	NN	denotes	Mcm
T4485	4232-4240	NN	denotes	helicase
T4490	4241-4243	IN	denotes	on
T4491	4244-4245	DT	denotes	a
T4493	4246-4252	NN	denotes	bubble
T4492	4253-4262	NN	denotes	substrate
T4494	4262-4263	.	denotes	.
T4495	4263-4373	sentence	denotes	In DNA helicase assays, Mcm4/6/7 complex did not show helicase activity on either Bub-10 or Bub-20 substrate.
T4496	4264-4266	IN	denotes	In
T4498	4267-4270	NN	denotes	DNA
T4499	4271-4279	NN	denotes	helicase
T4500	4280-4286	NNS	denotes	assays
T4501	4286-4288	,	denotes	,
T4502	4288-4292	NN	denotes	Mcm4
T4504	4292-4293	HYPH	denotes	/
T4505	4293-4294	CD	denotes	6
T4506	4294-4295	HYPH	denotes	/
T4507	4295-4296	CD	denotes	7
T4503	4297-4304	NN	denotes	complex
T4508	4305-4308	VBD	denotes	did
T4509	4309-4312	RB	denotes	not
T4497	4313-4317	VB	denotes	show
T4510	4318-4326	NN	denotes	helicase
T4511	4327-4335	NN	denotes	activity
T4512	4336-4338	IN	denotes	on
T4513	4339-4345	CC	denotes	either
T4514	4346-4349	NN	denotes	Bub
T4516	4349-4350	HYPH	denotes	-
T4517	4350-4352	CD	denotes	10
T4518	4353-4355	CC	denotes	or
T4519	4356-4359	NN	denotes	Bub
T4520	4359-4360	HYPH	denotes	-
T4521	4360-4362	CD	denotes	20
T4515	4363-4372	NN	denotes	substrate
T4522	4372-4373	.	denotes	.
T4523	4373-4490	sentence	denotes	The efficiency of displacement slightly improved as the length of the single-stranded segment increased up to 30 nt.
T4524	4374-4377	DT	denotes	The
T4525	4378-4388	NN	denotes	efficiency
T4527	4389-4391	IN	denotes	of
T4528	4392-4404	NN	denotes	displacement
T4529	4405-4413	RB	denotes	slightly
T4526	4414-4422	VBD	denotes	improved
T4530	4423-4425	IN	denotes	as
T4532	4426-4429	DT	denotes	the
T4533	4430-4436	NN	denotes	length
T4534	4437-4439	IN	denotes	of
T4535	4440-4443	DT	denotes	the
T4537	4444-4450	JJ	denotes	single
T4539	4450-4451	HYPH	denotes	-
T4538	4451-4459	VBN	denotes	stranded
T4536	4460-4467	NN	denotes	segment
T4531	4468-4477	VBD	denotes	increased
T4540	4478-4480	IN	denotes	up
T4542	4481-4483	IN	denotes	to
T4541	4484-4486	CD	denotes	30
T4543	4487-4489	NN	denotes	nt
T4544	4489-4490	.	denotes	.
T4545	4490-4714	sentence	denotes	On Bub-T40 and Bub-T50, on the other hand, significant displacement was observed, indicating that a single-stranded segment of at least 40 nt long is required for efficient helicase actions on bubble substrates (Figure 2B).
T4546	4491-4493	IN	denotes	On
T4548	4494-4497	NN	denotes	Bub
T4550	4497-4498	HYPH	denotes	-
T4549	4498-4501	NN	denotes	T40
T4551	4502-4505	CC	denotes	and
T4552	4506-4509	NN	denotes	Bub
T4554	4509-4510	HYPH	denotes	-
T4553	4510-4513	NN	denotes	T50
T4555	4513-4515	,	denotes	,
T4556	4515-4517	IN	denotes	on
T4557	4518-4521	DT	denotes	the
T4559	4522-4527	JJ	denotes	other
T4558	4528-4532	NN	denotes	hand
T4560	4532-4534	,	denotes	,
T4561	4534-4545	JJ	denotes	significant
T4562	4546-4558	NN	denotes	displacement
T4563	4559-4562	VBD	denotes	was
T4547	4563-4571	VBN	denotes	observed
T4564	4571-4573	,	denotes	,
T4565	4573-4583	VBG	denotes	indicating
T4566	4584-4588	IN	denotes	that
T4568	4589-4590	DT	denotes	a
T4570	4591-4597	JJ	denotes	single
T4572	4597-4598	HYPH	denotes	-
T4571	4598-4606	VBN	denotes	stranded
T4569	4607-4614	NN	denotes	segment
T4573	4615-4617	IN	denotes	of
T4574	4618-4620	RB	denotes	at
T4575	4621-4626	RBS	denotes	least
T4576	4627-4629	CD	denotes	40
T4577	4630-4632	NN	denotes	nt
T4578	4633-4637	JJ	denotes	long
T4579	4638-4640	VBZ	denotes	is
T4567	4641-4649	VBN	denotes	required
T4580	4650-4653	IN	denotes	for
T4581	4654-4663	JJ	denotes	efficient
T4583	4664-4672	NN	denotes	helicase
T4582	4673-4680	NNS	denotes	actions
T4584	4681-4683	IN	denotes	on
T4585	4684-4690	NN	denotes	bubble
T4586	4691-4701	NNS	denotes	substrates
T4587	4702-4703	-LRB-	denotes	(
T4589	4703-4709	NN	denotes	Figure
T4588	4710-4712	NN	denotes	2B
T4590	4712-4713	-RRB-	denotes	)
T4591	4713-4714	.	denotes	.
T4592	4714-4913	sentence	denotes	Taken together, the above results are consistent with the notion that the Mcm4/6/7 complex, loaded onto DNA as a double hexameric complex, efficiently unwinds the duplex region of the substrate DNA.
T4593	4715-4720	VBN	denotes	Taken
T4595	4721-4729	RB	denotes	together
T4596	4729-4731	,	denotes	,
T4597	4731-4734	DT	denotes	the
T4599	4735-4740	JJ	denotes	above
T4598	4741-4748	NNS	denotes	results
T4594	4749-4752	VBP	denotes	are
T4600	4753-4763	JJ	denotes	consistent
T4601	4764-4768	IN	denotes	with
T4602	4769-4772	DT	denotes	the
T4603	4773-4779	NN	denotes	notion
T4604	4780-4784	IN	denotes	that
T4606	4785-4788	DT	denotes	the
T4608	4789-4793	NN	denotes	Mcm4
T4609	4793-4794	HYPH	denotes	/
T4610	4794-4795	CD	denotes	6
T4611	4795-4796	HYPH	denotes	/
T4612	4796-4797	CD	denotes	7
T4607	4798-4805	NN	denotes	complex
T4613	4805-4807	,	denotes	,
T4614	4807-4813	VBN	denotes	loaded
T4615	4814-4818	IN	denotes	onto
T4616	4819-4822	NN	denotes	DNA
T4617	4823-4825	IN	denotes	as
T4618	4826-4827	DT	denotes	a
T4620	4828-4834	JJ	denotes	double
T4621	4835-4844	JJ	denotes	hexameric
T4619	4845-4852	NN	denotes	complex
T4622	4852-4854	,	denotes	,
T4623	4854-4865	RB	denotes	efficiently
T4605	4866-4873	VBZ	denotes	unwinds
T4624	4874-4877	DT	denotes	the
T4626	4878-4884	NN	denotes	duplex
T4625	4885-4891	NN	denotes	region
T4627	4892-4894	IN	denotes	of
T4628	4895-4898	DT	denotes	the
T4630	4899-4908	NN	denotes	substrate
T4629	4909-4912	NN	denotes	DNA
T4631	4912-4913	.	denotes	.
T4737	4915-4919	DT	denotes	Both
T4738	4920-4927	NNS	denotes	strands
T4740	4928-4930	IN	denotes	of
T4741	4931-4938	NN	denotes	thymine
T4743	4938-4939	HYPH	denotes	-
T4742	4939-4943	JJ	denotes	rich
T4745	4944-4950	JJ	denotes	single
T4747	4950-4951	HYPH	denotes	-
T4746	4951-4959	VBN	denotes	stranded
T4744	4960-4968	NNS	denotes	segments
T4748	4969-4972	VBP	denotes	are
T4739	4973-4981	VBN	denotes	required
T4749	4982-4985	IN	denotes	for
T4750	4986-4995	JJ	denotes	efficient
T4751	4996-5005	NN	denotes	unwinding
T4752	5006-5008	IN	denotes	of
T4753	5009-5015	NN	denotes	bubble
T4754	5016-5019	NN	denotes	DNA
T4755	5020-5022	IN	denotes	by
T4756	5023-5027	NN	denotes	Mcm4
T4757	5027-5028	HYPH	denotes	/
T4758	5028-5029	CD	denotes	6
T4759	5029-5030	HYPH	denotes	/
T4760	5030-5031	CD	denotes	7
T4761	5031-5172	sentence	denotes	Symmetric binding of the Mcm4/6/7 double hexamer to bubble suggests that each hexamer catalyzes unwinding of one of the two duplex segments.
T4762	5032-5041	JJ	denotes	Symmetric
T4763	5042-5049	NN	denotes	binding
T4765	5050-5052	IN	denotes	of
T4766	5053-5056	DT	denotes	the
T4768	5057-5061	NN	denotes	Mcm4
T4769	5061-5062	HYPH	denotes	/
T4770	5062-5063	CD	denotes	6
T4771	5063-5064	HYPH	denotes	/
T4772	5064-5065	CD	denotes	7
T4773	5066-5072	JJ	denotes	double
T4767	5073-5080	NN	denotes	hexamer
T4774	5081-5083	IN	denotes	to
T4775	5084-5090	NN	denotes	bubble
T4764	5091-5099	VBZ	denotes	suggests
T4776	5100-5104	IN	denotes	that
T4778	5105-5109	DT	denotes	each
T4779	5110-5117	NN	denotes	hexamer
T4777	5118-5127	VBZ	denotes	catalyzes
T4780	5128-5137	NN	denotes	unwinding
T4781	5138-5140	IN	denotes	of
T4782	5141-5144	CD	denotes	one
T4783	5145-5147	IN	denotes	of
T4784	5148-5151	DT	denotes	the
T4786	5152-5155	CD	denotes	two
T4787	5156-5162	NN	denotes	duplex
T4785	5163-5171	NNS	denotes	segments
T4788	5171-5172	.	denotes	.
T4789	5172-5268	sentence	denotes	We asked whether activation of both hexamers is required for unwinding of the bubble substrate.
T4790	5173-5175	PRP	denotes	We
T4791	5176-5181	VBD	denotes	asked
T4792	5182-5189	IN	denotes	whether
T4794	5190-5200	NN	denotes	activation
T4795	5201-5203	IN	denotes	of
T4796	5204-5208	DT	denotes	both
T4797	5209-5217	NNS	denotes	hexamers
T4798	5218-5220	VBZ	denotes	is
T4793	5221-5229	VBN	denotes	required
T4799	5230-5233	IN	denotes	for
T4800	5234-5243	NN	denotes	unwinding
T4801	5244-5246	IN	denotes	of
T4802	5247-5250	DT	denotes	the
T4804	5251-5257	NN	denotes	bubble
T4803	5258-5267	NN	denotes	substrate
T4805	5267-5268	.	denotes	.
T4806	5268-5415	sentence	denotes	The preferred bubble substrate which is efficiently unwound by Mcm4/6/7 contains thymine stretches on both strands of the single-stranded segment.
T4807	5269-5272	DT	denotes	The
T4809	5273-5282	JJ	denotes	preferred
T4810	5283-5289	NN	denotes	bubble
T4808	5290-5299	NN	denotes	substrate
T4812	5300-5305	WDT	denotes	which
T4814	5306-5308	VBZ	denotes	is
T4815	5309-5320	RB	denotes	efficiently
T4813	5321-5328	VBN	denotes	unwound
T4816	5329-5331	IN	denotes	by
T4817	5332-5336	NN	denotes	Mcm4
T4818	5336-5337	HYPH	denotes	/
T4819	5337-5338	CD	denotes	6
T4820	5338-5339	HYPH	denotes	/
T4821	5339-5340	CD	denotes	7
T4811	5341-5349	VBZ	denotes	contains
T4822	5350-5357	NN	denotes	thymine
T4823	5358-5367	NNS	denotes	stretches
T4824	5368-5370	IN	denotes	on
T4825	5371-5375	DT	denotes	both
T4826	5376-5383	NNS	denotes	strands
T4827	5384-5386	IN	denotes	of
T4828	5387-5390	DT	denotes	the
T4830	5391-5397	JJ	denotes	single
T4832	5397-5398	HYPH	denotes	-
T4831	5398-5406	VBN	denotes	stranded
T4829	5407-5414	NN	denotes	segment
T4833	5414-5415	.	denotes	.
T4834	5415-5647	sentence	denotes	We constructed a hetero-bubble (Bub66/T–G-rich) in which one strand is T-rich (same as Bub66/T-rich) and the other strand is G-rich (same as Bub66/G-rich) (13), and helicase assays were conducted with increasing amount of Mcm4/6/7.
T4835	5416-5418	PRP	denotes	We
T4836	5419-5430	VBD	denotes	constructed
T4837	5431-5432	DT	denotes	a
T4838	5433-5446	JJ	denotes	hetero-bubble
T4839	5447-5448	-LRB-	denotes	(
T4840	5448-5453	NN	denotes	Bub66
T4842	5453-5454	HYPH	denotes	/
T4843	5454-5455	NN	denotes	T
T4844	5455-5456	HYPH	denotes	–
T4841	5456-5457	NN	denotes	G
T4845	5457-5458	HYPH	denotes	-
T4846	5458-5462	JJ	denotes	rich
T4847	5462-5463	-RRB-	denotes	)
T4848	5464-5466	IN	denotes	in
T4850	5467-5472	WDT	denotes	which
T4851	5473-5476	CD	denotes	one
T4852	5477-5483	NN	denotes	strand
T4849	5484-5486	VBZ	denotes	is
T4853	5487-5488	NN	denotes	T
T4855	5488-5489	HYPH	denotes	-
T4854	5489-5493	JJ	denotes	rich
T4856	5494-5495	-LRB-	denotes	(
T4857	5495-5499	JJ	denotes	same
T4858	5500-5502	IN	denotes	as
T4859	5503-5508	NN	denotes	Bub66
T4861	5508-5509	HYPH	denotes	/
T4860	5509-5510	NN	denotes	T
T4862	5510-5511	HYPH	denotes	-
T4863	5511-5515	JJ	denotes	rich
T4864	5515-5516	-RRB-	denotes	)
T4865	5517-5520	CC	denotes	and
T4866	5521-5524	DT	denotes	the
T4868	5525-5530	JJ	denotes	other
T4867	5531-5537	NN	denotes	strand
T4869	5538-5540	VBZ	denotes	is
T4870	5541-5542	NN	denotes	G
T4872	5542-5543	HYPH	denotes	-
T4871	5543-5547	JJ	denotes	rich
T4873	5548-5549	-LRB-	denotes	(
T4874	5549-5553	JJ	denotes	same
T4875	5554-5556	IN	denotes	as
T4876	5557-5562	NN	denotes	Bub66
T4878	5562-5563	HYPH	denotes	/
T4877	5563-5564	NN	denotes	G
T4879	5564-5565	HYPH	denotes	-
T4880	5565-5569	JJ	denotes	rich
T4881	5569-5570	-RRB-	denotes	)
T4882	5571-5572	-LRB-	denotes	(
T4883	5572-5574	CD	denotes	13
T4884	5574-5575	-RRB-	denotes	)
T4885	5575-5577	,	denotes	,
T4886	5577-5580	CC	denotes	and
T4887	5581-5589	NN	denotes	helicase
T4888	5590-5596	NNS	denotes	assays
T4890	5597-5601	VBD	denotes	were
T4889	5602-5611	VBN	denotes	conducted
T4891	5612-5616	IN	denotes	with
T4892	5617-5627	VBG	denotes	increasing
T4893	5628-5634	NN	denotes	amount
T4894	5635-5637	IN	denotes	of
T4895	5638-5642	NN	denotes	Mcm4
T4896	5642-5643	HYPH	denotes	/
T4897	5643-5644	CD	denotes	6
T4898	5644-5645	HYPH	denotes	/
T4899	5645-5646	CD	denotes	7
T4900	5646-5647	.	denotes	.
T4901	5647-5736	sentence	denotes	Only weak unwinding was detected on this substrate compared to Bub66/T-rich (Figure 3A).
T4902	5648-5652	RB	denotes	Only
T4904	5653-5657	JJ	denotes	weak
T4903	5658-5667	NN	denotes	unwinding
T4906	5668-5671	VBD	denotes	was
T4905	5672-5680	VBN	denotes	detected
T4907	5681-5683	IN	denotes	on
T4908	5684-5688	DT	denotes	this
T4909	5689-5698	NN	denotes	substrate
T4910	5699-5707	VBN	denotes	compared
T4911	5708-5710	IN	denotes	to
T4912	5711-5716	NN	denotes	Bub66
T4914	5716-5717	HYPH	denotes	/
T4913	5717-5718	NN	denotes	T
T4915	5718-5719	HYPH	denotes	-
T4916	5719-5723	JJ	denotes	rich
T4917	5724-5725	-LRB-	denotes	(
T4919	5725-5731	NN	denotes	Figure
T4918	5732-5734	NN	denotes	3A
T4920	5734-5735	-RRB-	denotes	)
T4921	5735-5736	.	denotes	.
T4922	5736-5927	sentence	denotes	Since Mcm4/6/7 can bind to Bub66/T–G-rich and forms a double hexameric complex (data not shown), the defective unwinding is not due to inefficient loading of the helicase onto the substrate.
T4923	5737-5742	IN	denotes	Since
T4925	5743-5747	NN	denotes	Mcm4
T4926	5747-5748	HYPH	denotes	/
T4927	5748-5749	CD	denotes	6
T4928	5749-5750	HYPH	denotes	/
T4929	5750-5751	CD	denotes	7
T4930	5752-5755	MD	denotes	can
T4924	5756-5760	VB	denotes	bind
T4932	5761-5763	IN	denotes	to
T4933	5764-5769	NN	denotes	Bub66
T4935	5769-5770	HYPH	denotes	/
T4936	5770-5771	NN	denotes	T
T4937	5771-5772	HYPH	denotes	–
T4934	5772-5773	NN	denotes	G
T4938	5773-5774	HYPH	denotes	-
T4939	5774-5778	JJ	denotes	rich
T4940	5779-5782	CC	denotes	and
T4941	5783-5788	VBZ	denotes	forms
T4942	5789-5790	DT	denotes	a
T4944	5791-5797	JJ	denotes	double
T4945	5798-5807	JJ	denotes	hexameric
T4943	5808-5815	NN	denotes	complex
T4946	5816-5817	-LRB-	denotes	(
T4948	5817-5821	NNS	denotes	data
T4949	5822-5825	RB	denotes	not
T4947	5826-5831	VBN	denotes	shown
T4950	5831-5832	-RRB-	denotes	)
T4951	5832-5834	,	denotes	,
T4952	5834-5837	DT	denotes	the
T4954	5838-5847	JJ	denotes	defective
T4953	5848-5857	NN	denotes	unwinding
T4931	5858-5860	VBZ	denotes	is
T4955	5861-5864	RB	denotes	not
T4956	5865-5868	IN	denotes	due
T4957	5869-5871	IN	denotes	to
T4958	5872-5883	JJ	denotes	inefficient
T4959	5884-5891	NN	denotes	loading
T4960	5892-5894	IN	denotes	of
T4961	5895-5898	DT	denotes	the
T4962	5899-5907	NN	denotes	helicase
T4963	5908-5912	IN	denotes	onto
T4964	5913-5916	DT	denotes	the
T4965	5917-5926	NN	denotes	substrate
T4966	5926-5927	.	denotes	.
T4967	5927-6225	sentence	denotes	If one hexamer interacting with the thymine-rich strand dissociates from the other hexamer and displaces only one of the duplex segments, then it would result in generation of Y-fork structures which could not be further displaced due to the presence of G-rich 3′-tail and would appear on the gel.
T4968	5928-5930	IN	denotes	If
T4970	5931-5934	CD	denotes	one
T4971	5935-5942	NN	denotes	hexamer
T4972	5943-5954	VBG	denotes	interacting
T4973	5955-5959	IN	denotes	with
T4974	5960-5963	DT	denotes	the
T4976	5964-5971	NN	denotes	thymine
T4978	5971-5972	HYPH	denotes	-
T4977	5972-5976	JJ	denotes	rich
T4975	5977-5983	NN	denotes	strand
T4969	5984-5995	VBZ	denotes	dissociates
T4980	5996-6000	IN	denotes	from
T4981	6001-6004	DT	denotes	the
T4983	6005-6010	JJ	denotes	other
T4982	6011-6018	NN	denotes	hexamer
T4984	6019-6022	CC	denotes	and
T4985	6023-6032	VBZ	denotes	displaces
T4986	6033-6037	RB	denotes	only
T4987	6038-6041	CD	denotes	one
T4988	6042-6044	IN	denotes	of
T4989	6045-6048	DT	denotes	the
T4991	6049-6055	NN	denotes	duplex
T4990	6056-6064	NNS	denotes	segments
T4992	6064-6066	,	denotes	,
T4993	6066-6070	RB	denotes	then
T4994	6071-6073	PRP	denotes	it
T4995	6074-6079	MD	denotes	would
T4979	6080-6086	VB	denotes	result
T4996	6087-6089	IN	denotes	in
T4997	6090-6100	NN	denotes	generation
T4998	6101-6103	IN	denotes	of
T4999	6104-6105	NN	denotes	Y
T5001	6105-6106	HYPH	denotes	-
T5000	6106-6110	NN	denotes	fork
T5002	6111-6121	NNS	denotes	structures
T5003	6122-6127	WDT	denotes	which
T5005	6128-6133	MD	denotes	could
T5006	6134-6137	RB	denotes	not
T5007	6138-6140	VB	denotes	be
T5008	6141-6148	RB	denotes	further
T5004	6149-6158	VBN	denotes	displaced
T5009	6159-6162	IN	denotes	due
T5010	6163-6165	IN	denotes	to
T5011	6166-6169	DT	denotes	the
T5012	6170-6178	NN	denotes	presence
T5013	6179-6181	IN	denotes	of
T5014	6182-6183	NN	denotes	G
T5016	6183-6184	HYPH	denotes	-
T5015	6184-6188	JJ	denotes	rich
T5018	6189-6190	CD	denotes	3
T5019	6190-6191	SYM	denotes	′
T5020	6191-6192	HYPH	denotes	-
T5017	6192-6196	NN	denotes	tail
T5021	6197-6200	CC	denotes	and
T5022	6201-6206	MD	denotes	would
T5023	6207-6213	VB	denotes	appear
T5024	6214-6216	IN	denotes	on
T5025	6217-6220	DT	denotes	the
T5026	6221-6224	NN	denotes	gel
T5027	6224-6225	.	denotes	.
T5028	6225-6451	sentence	denotes	However, we did not detect such intermediates during the course of the experiment (Figure 3B), suggesting that bubble can be displaced efficiently only when two molecules of the Mcm4/6/7 hexamers are simultaneously activated.
T5029	6226-6233	RB	denotes	However
T5031	6233-6235	,	denotes	,
T5032	6235-6237	PRP	denotes	we
T5033	6238-6241	VBD	denotes	did
T5034	6242-6245	RB	denotes	not
T5030	6246-6252	VB	denotes	detect
T5035	6253-6257	JJ	denotes	such
T5036	6258-6271	NNS	denotes	intermediates
T5037	6272-6278	IN	denotes	during
T5038	6279-6282	DT	denotes	the
T5039	6283-6289	NN	denotes	course
T5040	6290-6292	IN	denotes	of
T5041	6293-6296	DT	denotes	the
T5042	6297-6307	NN	denotes	experiment
T5043	6308-6309	-LRB-	denotes	(
T5045	6309-6315	NN	denotes	Figure
T5044	6316-6318	NN	denotes	3B
T5046	6318-6319	-RRB-	denotes	)
T5047	6319-6321	,	denotes	,
T5048	6321-6331	VBG	denotes	suggesting
T5049	6332-6336	IN	denotes	that
T5051	6337-6343	NN	denotes	bubble
T5052	6344-6347	MD	denotes	can
T5053	6348-6350	VB	denotes	be
T5050	6351-6360	VBN	denotes	displaced
T5054	6361-6372	RB	denotes	efficiently
T5055	6373-6377	RB	denotes	only
T5057	6378-6382	WRB	denotes	when
T5058	6383-6386	CD	denotes	two
T5059	6387-6396	NNS	denotes	molecules
T5060	6397-6399	IN	denotes	of
T5061	6400-6403	DT	denotes	the
T5063	6404-6408	NN	denotes	Mcm4
T5064	6408-6409	HYPH	denotes	/
T5065	6409-6410	CD	denotes	6
T5066	6410-6411	HYPH	denotes	/
T5067	6411-6412	CD	denotes	7
T5062	6413-6421	NNS	denotes	hexamers
T5068	6422-6425	VBP	denotes	are
T5069	6426-6440	RB	denotes	simultaneously
T5056	6441-6450	VBN	denotes	activated
T5070	6450-6451	.	denotes	.
T5260	6453-6460	NN	denotes	Binding
T5262	6461-6464	CC	denotes	and
T5263	6465-6473	NN	denotes	helicase
T5261	6474-6481	NNS	denotes	actions
T5264	6482-6484	IN	denotes	of
T5265	6485-6489	NN	denotes	Mcm4
T5266	6489-6490	HYPH	denotes	/
T5267	6490-6491	CD	denotes	6
T5268	6491-6492	HYPH	denotes	/
T5269	6492-6493	CD	denotes	7
T5270	6494-6496	IN	denotes	on
T5271	6497-6503	VBN	denotes	forked
T5273	6504-6507	CC	denotes	and
T5274	6508-6509	CD	denotes	3
T5276	6509-6510	SYM	denotes	′
T5277	6510-6511	HYPH	denotes	-
T5278	6512-6514	CC	denotes	or
T5279	6515-6516	CD	denotes	5
T5280	6516-6517	SYM	denotes	′
T5281	6517-6518	HYPH	denotes	-
T5275	6518-6527	NN	denotes	extension
T5272	6528-6538	NNS	denotes	substrates
T5282	6538-6841	sentence	denotes	In order to examine more precisely the interaction and helicase actions with forked substrates, Y-fork substrates containing a nascent leading strand (A-fork[3′]), nascent lagging strand (A-fork[5′]), or both of them (A-fork[3′,5′]), as well as 5′-extension or 3′-extension structures were constructed.
T5283	6539-6541	IN	denotes	In
T5285	6542-6547	NN	denotes	order
T5286	6548-6550	TO	denotes	to
T5287	6551-6558	VB	denotes	examine
T5288	6559-6563	RBR	denotes	more
T5289	6564-6573	RB	denotes	precisely
T5290	6574-6577	DT	denotes	the
T5291	6578-6589	NN	denotes	interaction
T5292	6590-6593	CC	denotes	and
T5293	6594-6602	NN	denotes	helicase
T5294	6603-6610	NNS	denotes	actions
T5295	6611-6615	IN	denotes	with
T5296	6616-6622	VBN	denotes	forked
T5297	6623-6633	NNS	denotes	substrates
T5298	6633-6635	,	denotes	,
T5299	6635-6636	NN	denotes	Y
T5301	6636-6637	HYPH	denotes	-
T5300	6637-6641	NN	denotes	fork
T5302	6642-6652	NNS	denotes	substrates
T5303	6653-6663	VBG	denotes	containing
T5304	6664-6665	DT	denotes	a
T5306	6666-6673	JJ	denotes	nascent
T5307	6674-6681	VBG	denotes	leading
T5305	6682-6688	NN	denotes	strand
T5308	6689-6690	-LRB-	denotes	(
T5309	6690-6691	NN	denotes	A
T5311	6691-6692	HYPH	denotes	-
T5310	6692-6696	NN	denotes	fork
T5312	6696-6697	-LRB-	denotes	[
T5313	6697-6698	CD	denotes	3
T5314	6698-6699	SYM	denotes	′
T5315	6699-6700	-RRB-	denotes	]
T5316	6700-6701	-RRB-	denotes	)
T5317	6701-6703	,	denotes	,
T5318	6703-6710	JJ	denotes	nascent
T5320	6711-6718	VBG	denotes	lagging
T5319	6719-6725	NN	denotes	strand
T5321	6726-6727	-LRB-	denotes	(
T5322	6727-6728	NN	denotes	A
T5324	6728-6729	HYPH	denotes	-
T5323	6729-6733	NN	denotes	fork
T5325	6733-6734	-LRB-	denotes	[
T5326	6734-6735	CD	denotes	5
T5327	6735-6736	SYM	denotes	′
T5328	6736-6737	-RRB-	denotes	]
T5329	6737-6738	-RRB-	denotes	)
T5330	6738-6740	,	denotes	,
T5331	6740-6742	CC	denotes	or
T5332	6743-6747	DT	denotes	both
T5333	6748-6750	IN	denotes	of
T5334	6751-6755	PRP	denotes	them
T5335	6756-6757	-LRB-	denotes	(
T5336	6757-6758	NN	denotes	A
T5338	6758-6759	HYPH	denotes	-
T5337	6759-6763	NN	denotes	fork
T5340	6763-6764	-LRB-	denotes	[
T5341	6764-6765	CD	denotes	3
T5342	6765-6766	SYM	denotes	′
T5343	6766-6767	,	denotes	,
T5344	6767-6768	CD	denotes	5
T5345	6768-6769	SYM	denotes	′
T5346	6769-6770	-RRB-	denotes	]
T5347	6770-6771	-RRB-	denotes	)
T5348	6771-6773	,	denotes	,
T5349	6773-6775	RB	denotes	as
T5351	6776-6780	RB	denotes	well
T5350	6781-6783	IN	denotes	as
T5352	6784-6785	CD	denotes	5
T5354	6785-6786	SYM	denotes	′
T5355	6786-6787	HYPH	denotes	-
T5353	6787-6796	NN	denotes	extension
T5356	6797-6799	CC	denotes	or
T5357	6800-6801	CD	denotes	3
T5359	6801-6802	SYM	denotes	′
T5360	6802-6803	HYPH	denotes	-
T5358	6803-6812	NN	denotes	extension
T5339	6813-6823	NNS	denotes	structures
T5361	6824-6828	VBD	denotes	were
T5284	6829-6840	VBN	denotes	constructed
T5362	6840-6841	.	denotes	.
T5363	6841-6965	sentence	denotes	Mcm4/6/7 bound to A-fork[3′] or A-fork[5′] with affinity similar to that of a simple Y-fork (Figure 4A and data not shown).
T5364	6842-6846	NN	denotes	Mcm4
T5366	6846-6847	HYPH	denotes	/
T5367	6847-6848	CD	denotes	6
T5368	6848-6849	HYPH	denotes	/
T5369	6849-6850	CD	denotes	7
T5365	6851-6856	VBD	denotes	bound
T5370	6857-6859	IN	denotes	to
T5371	6860-6861	NN	denotes	A
T5373	6861-6862	HYPH	denotes	-
T5372	6862-6866	NN	denotes	fork
T5374	6866-6867	-LRB-	denotes	[
T5375	6867-6868	CD	denotes	3
T5376	6868-6869	SYM	denotes	′
T5377	6869-6870	-RRB-	denotes	]
T5378	6871-6873	CC	denotes	or
T5379	6874-6875	NN	denotes	A
T5381	6875-6876	HYPH	denotes	-
T5380	6876-6880	NN	denotes	fork
T5382	6880-6881	-LRB-	denotes	[
T5383	6881-6882	CD	denotes	5
T5384	6882-6883	SYM	denotes	′
T5385	6883-6884	-RRB-	denotes	]
T5386	6885-6889	IN	denotes	with
T5387	6890-6898	NN	denotes	affinity
T5388	6899-6906	JJ	denotes	similar
T5389	6907-6909	IN	denotes	to
T5390	6910-6914	DT	denotes	that
T5391	6915-6917	IN	denotes	of
T5392	6918-6919	DT	denotes	a
T5394	6920-6926	JJ	denotes	simple
T5395	6927-6928	NN	denotes	Y
T5396	6928-6929	HYPH	denotes	-
T5393	6929-6933	NN	denotes	fork
T5397	6934-6935	-LRB-	denotes	(
T5399	6935-6941	NN	denotes	Figure
T5398	6942-6944	NN	denotes	4A
T5400	6945-6948	CC	denotes	and
T5401	6949-6953	NNS	denotes	data
T5403	6954-6957	RB	denotes	not
T5402	6958-6963	VBN	denotes	shown
T5404	6963-6964	-RRB-	denotes	)
T5405	6964-6965	.	denotes	.
T5406	6965-7101	sentence	denotes	In contrast, PriA helicase, used as a control, bound to A-fork[3′] more efficiently than to A-fork[5′], as reported previously (23,25).
T5407	6966-6968	IN	denotes	In
T5409	6969-6977	NN	denotes	contrast
T5410	6977-6979	,	denotes	,
T5411	6979-6983	NN	denotes	PriA
T5412	6984-6992	NN	denotes	helicase
T5413	6992-6994	,	denotes	,
T5414	6994-6998	VBN	denotes	used
T5415	6999-7001	IN	denotes	as
T5416	7002-7003	DT	denotes	a
T5417	7004-7011	NN	denotes	control
T5418	7011-7013	,	denotes	,
T5408	7013-7018	VBD	denotes	bound
T5419	7019-7021	IN	denotes	to
T5420	7022-7023	NN	denotes	A
T5422	7023-7024	HYPH	denotes	-
T5421	7024-7028	NN	denotes	fork
T5423	7028-7029	-LRB-	denotes	[
T5424	7029-7030	CD	denotes	3
T5425	7030-7031	SYM	denotes	′
T5426	7031-7032	-RRB-	denotes	]
T5427	7033-7037	RBR	denotes	more
T5428	7038-7049	RB	denotes	efficiently
T5429	7050-7054	IN	denotes	than
T5430	7055-7057	IN	denotes	to
T5431	7058-7059	NN	denotes	A
T5433	7059-7060	HYPH	denotes	-
T5432	7060-7064	NN	denotes	fork
T5434	7064-7065	-LRB-	denotes	[
T5435	7065-7066	CD	denotes	5
T5436	7066-7067	SYM	denotes	′
T5437	7067-7068	-RRB-	denotes	]
T5438	7068-7070	,	denotes	,
T5439	7070-7072	IN	denotes	as
T5440	7073-7081	VBN	denotes	reported
T5441	7082-7092	RB	denotes	previously
T5442	7093-7094	-LRB-	denotes	(
T5444	7094-7096	CD	denotes	23
T5445	7096-7097	,	denotes	,
T5443	7097-7099	CD	denotes	25
T5446	7099-7100	-RRB-	denotes	)
T5447	7100-7101	.	denotes	.
T5448	7101-7244	sentence	denotes	Mcm4/6/7 bound also to 5′-extension and 3′-extension, albeit with slightly reduced affinity compared to A-fork[3′] and A-fork[5′] (Figure 4A).
T5449	7102-7106	NN	denotes	Mcm4
T5451	7106-7107	HYPH	denotes	/
T5452	7107-7108	CD	denotes	6
T5453	7108-7109	HYPH	denotes	/
T5454	7109-7110	CD	denotes	7
T5450	7111-7116	VBD	denotes	bound
T5455	7117-7121	RB	denotes	also
T5456	7122-7124	IN	denotes	to
T5457	7125-7126	CD	denotes	5
T5459	7126-7127	SYM	denotes	′
T5460	7127-7128	HYPH	denotes	-
T5458	7128-7137	NN	denotes	extension
T5461	7138-7141	CC	denotes	and
T5462	7142-7143	CD	denotes	3
T5464	7143-7144	SYM	denotes	′
T5465	7144-7145	HYPH	denotes	-
T5463	7145-7154	NN	denotes	extension
T5466	7154-7156	,	denotes	,
T5467	7156-7162	IN	denotes	albeit
T5468	7163-7167	IN	denotes	with
T5469	7168-7176	RB	denotes	slightly
T5470	7177-7184	VBN	denotes	reduced
T5471	7185-7193	NN	denotes	affinity
T5472	7194-7202	VBN	denotes	compared
T5473	7203-7205	IN	denotes	to
T5474	7206-7207	NN	denotes	A
T5476	7207-7208	HYPH	denotes	-
T5475	7208-7212	NN	denotes	fork
T5477	7212-7213	-LRB-	denotes	[
T5478	7213-7214	CD	denotes	3
T5479	7214-7215	SYM	denotes	′
T5480	7215-7216	-RRB-	denotes	]
T5481	7217-7220	CC	denotes	and
T5482	7221-7222	NN	denotes	A
T5484	7222-7223	HYPH	denotes	-
T5483	7223-7227	NN	denotes	fork
T5485	7227-7228	-LRB-	denotes	[
T5486	7228-7229	CD	denotes	5
T5487	7229-7230	SYM	denotes	′
T5488	7230-7231	-RRB-	denotes	]
T5489	7232-7233	-LRB-	denotes	(
T5491	7233-7239	NN	denotes	Figure
T5490	7240-7242	NN	denotes	4A
T5492	7242-7243	-RRB-	denotes	)
T5493	7243-7244	.	denotes	.
T5494	7244-7346	sentence	denotes	Mcm4/6/7 bound to A-fork[3′,5′] with much lower affinity, and the complex gave rise to a single band.
T5495	7245-7249	NN	denotes	Mcm4
T5497	7249-7250	HYPH	denotes	/
T5498	7250-7251	CD	denotes	6
T5499	7251-7252	HYPH	denotes	/
T5500	7252-7253	CD	denotes	7
T5496	7254-7259	VBD	denotes	bound
T5501	7260-7262	IN	denotes	to
T5502	7263-7264	NN	denotes	A
T5504	7264-7265	HYPH	denotes	-
T5503	7265-7269	NN	denotes	fork
T5505	7269-7270	-LRB-	denotes	[
T5506	7270-7271	CD	denotes	3
T5507	7271-7272	SYM	denotes	′
T5508	7272-7273	,	denotes	,
T5509	7273-7274	CD	denotes	5
T5510	7274-7275	SYM	denotes	′
T5511	7275-7276	-RRB-	denotes	]
T5512	7277-7281	IN	denotes	with
T5513	7282-7286	RB	denotes	much
T5514	7287-7292	JJR	denotes	lower
T5515	7293-7301	NN	denotes	affinity
T5516	7301-7303	,	denotes	,
T5517	7303-7306	CC	denotes	and
T5518	7307-7310	DT	denotes	the
T5519	7311-7318	NN	denotes	complex
T5520	7319-7323	VBD	denotes	gave
T5521	7324-7328	NN	denotes	rise
T5522	7329-7331	IN	denotes	to
T5523	7332-7333	DT	denotes	a
T5525	7334-7340	JJ	denotes	single
T5524	7341-7345	NN	denotes	band
T5526	7345-7346	.	denotes	.
T5527	7346-7528	sentence	denotes	These findings indicate that the Mcm4/6/7 complex is loaded onto DNA preferentially and efficiently through single-stranded region, regardless of the presence of 3′- or 5′-terminus.
T5528	7347-7352	DT	denotes	These
T5529	7353-7361	NNS	denotes	findings
T5530	7362-7370	VBP	denotes	indicate
T5531	7371-7375	IN	denotes	that
T5533	7376-7379	DT	denotes	the
T5535	7380-7384	NN	denotes	Mcm4
T5536	7384-7385	HYPH	denotes	/
T5537	7385-7386	CD	denotes	6
T5538	7386-7387	HYPH	denotes	/
T5539	7387-7388	CD	denotes	7
T5534	7389-7396	NN	denotes	complex
T5540	7397-7399	VBZ	denotes	is
T5532	7400-7406	VBN	denotes	loaded
T5541	7407-7411	IN	denotes	onto
T5542	7412-7415	NN	denotes	DNA
T5543	7416-7430	RB	denotes	preferentially
T5544	7431-7434	CC	denotes	and
T5545	7435-7446	RB	denotes	efficiently
T5546	7447-7454	IN	denotes	through
T5547	7455-7461	JJ	denotes	single
T5549	7461-7462	HYPH	denotes	-
T5548	7462-7470	VBN	denotes	stranded
T5550	7471-7477	NN	denotes	region
T5551	7477-7479	,	denotes	,
T5552	7479-7489	RB	denotes	regardless
T5553	7490-7492	IN	denotes	of
T5554	7493-7496	DT	denotes	the
T5555	7497-7505	NN	denotes	presence
T5556	7506-7508	IN	denotes	of
T5557	7509-7510	CD	denotes	3
T5559	7510-7511	SYM	denotes	′
T5560	7511-7512	HYPH	denotes	-
T5561	7513-7515	CC	denotes	or
T5562	7516-7517	CD	denotes	5
T5563	7517-7518	SYM	denotes	′
T5564	7518-7519	HYPH	denotes	-
T5558	7519-7527	NN	denotes	terminus
T5565	7527-7528	.	denotes	.
T5566	7528-7712	sentence	denotes	In contrast, only the substrate containing a 3′ single-stranded DNA tail (A-fork[5′]) was efficiently unwound and A-fork[3′] or A-fork[3′,5′], was not unwound by Mcm4/6/7 (Figure 4B).
T5567	7529-7531	IN	denotes	In
T5569	7532-7540	NN	denotes	contrast
T5570	7540-7542	,	denotes	,
T5571	7542-7546	RB	denotes	only
T5573	7547-7550	DT	denotes	the
T5572	7551-7560	NN	denotes	substrate
T5574	7561-7571	VBG	denotes	containing
T5575	7572-7573	DT	denotes	a
T5577	7574-7575	CD	denotes	3
T5578	7575-7576	SYM	denotes	′
T5579	7577-7583	JJ	denotes	single
T5581	7583-7584	HYPH	denotes	-
T5580	7584-7592	VBN	denotes	stranded
T5582	7593-7596	NN	denotes	DNA
T5576	7597-7601	NN	denotes	tail
T5583	7602-7603	-LRB-	denotes	(
T5585	7603-7604	NN	denotes	A
T5586	7604-7605	HYPH	denotes	-
T5584	7605-7609	NN	denotes	fork
T5587	7609-7610	-LRB-	denotes	[
T5588	7610-7611	CD	denotes	5
T5589	7611-7612	SYM	denotes	′
T5590	7612-7613	-RRB-	denotes	]
T5591	7613-7614	-RRB-	denotes	)
T5592	7615-7618	VBD	denotes	was
T5593	7619-7630	RB	denotes	efficiently
T5568	7631-7638	VBN	denotes	unwound
T5594	7639-7642	CC	denotes	and
T5595	7643-7644	NN	denotes	A
T5597	7644-7645	HYPH	denotes	-
T5596	7645-7649	NN	denotes	fork
T5599	7649-7650	-LRB-	denotes	[
T5600	7650-7651	CD	denotes	3
T5601	7651-7652	SYM	denotes	′
T5602	7652-7653	-RRB-	denotes	]
T5603	7654-7656	CC	denotes	or
T5604	7657-7658	NN	denotes	A
T5606	7658-7659	HYPH	denotes	-
T5605	7659-7663	NN	denotes	fork
T5607	7663-7664	-LRB-	denotes	[
T5608	7664-7665	CD	denotes	3
T5609	7665-7666	SYM	denotes	′
T5610	7666-7667	,	denotes	,
T5611	7667-7668	CD	denotes	5
T5612	7668-7669	SYM	denotes	′
T5613	7669-7670	-RRB-	denotes	]
T5614	7670-7672	,	denotes	,
T5615	7672-7675	VBD	denotes	was
T5616	7676-7679	RB	denotes	not
T5598	7680-7687	VBN	denotes	unwound
T5617	7688-7690	IN	denotes	by
T5618	7691-7695	NN	denotes	Mcm4
T5619	7695-7696	HYPH	denotes	/
T5620	7696-7697	CD	denotes	6
T5621	7697-7698	HYPH	denotes	/
T5622	7698-7699	CD	denotes	7
T5623	7700-7701	-LRB-	denotes	(
T5625	7701-7707	NN	denotes	Figure
T5624	7708-7710	NN	denotes	4B
T5626	7710-7711	-RRB-	denotes	)
T5627	7711-7712	.	denotes	.
T5628	7712-7889	sentence	denotes	Absence of helicase actions on A-fork[3′,5′] composed only of duplex regions is expected since double-stranded DNA is not able to activate the ATPase activity of Mcm4/6/7 (10).
T5629	7713-7720	NN	denotes	Absence
T5631	7721-7723	IN	denotes	of
T5632	7724-7732	NN	denotes	helicase
T5633	7733-7740	NNS	denotes	actions
T5634	7741-7743	IN	denotes	on
T5635	7744-7745	NN	denotes	A
T5637	7745-7746	HYPH	denotes	-
T5636	7746-7750	NN	denotes	fork
T5638	7750-7751	-LRB-	denotes	[
T5639	7751-7752	CD	denotes	3
T5640	7752-7753	SYM	denotes	′
T5641	7753-7754	,	denotes	,
T5642	7754-7755	CD	denotes	5
T5643	7755-7756	SYM	denotes	′
T5644	7756-7757	-RRB-	denotes	]
T5645	7758-7766	VBN	denotes	composed
T5646	7767-7771	RB	denotes	only
T5647	7772-7774	IN	denotes	of
T5648	7775-7781	NN	denotes	duplex
T5649	7782-7789	NNS	denotes	regions
T5650	7790-7792	VBZ	denotes	is
T5630	7793-7801	VBN	denotes	expected
T5651	7802-7807	IN	denotes	since
T5653	7808-7814	JJ	denotes	double
T5655	7814-7815	HYPH	denotes	-
T5654	7815-7823	VBN	denotes	stranded
T5656	7824-7827	NN	denotes	DNA
T5652	7828-7830	VBZ	denotes	is
T5657	7831-7834	RB	denotes	not
T5658	7835-7839	JJ	denotes	able
T5659	7840-7842	TO	denotes	to
T5660	7843-7851	VB	denotes	activate
T5661	7852-7855	DT	denotes	the
T5663	7856-7862	NN	denotes	ATPase
T5662	7863-7871	NN	denotes	activity
T5664	7872-7874	IN	denotes	of
T5665	7875-7879	NN	denotes	Mcm4
T5666	7879-7880	HYPH	denotes	/
T5667	7880-7881	CD	denotes	6
T5668	7881-7882	HYPH	denotes	/
T5669	7882-7883	CD	denotes	7
T5670	7884-7885	-LRB-	denotes	(
T5671	7885-7887	CD	denotes	10
T5672	7887-7888	-RRB-	denotes	)
T5673	7888-7889	.	denotes	.
T5674	7889-8140	sentence	denotes	In contrast, the PriA, a structure-specific helicase used as a control, binds at the fork junction and unwinds the fork and the nascent lagging strand DNA on A-fork[5′], or unwinds the nascent lagging strand on A-fork[3′,5′], as reported before (25).
T5675	7890-7892	IN	denotes	In
T5677	7893-7901	NN	denotes	contrast
T5678	7901-7903	,	denotes	,
T5679	7903-7906	DT	denotes	the
T5680	7907-7911	NN	denotes	PriA
T5681	7911-7913	,	denotes	,
T5682	7913-7914	DT	denotes	a
T5684	7915-7924	NN	denotes	structure
T5686	7924-7925	HYPH	denotes	-
T5685	7925-7933	JJ	denotes	specific
T5683	7934-7942	NN	denotes	helicase
T5687	7943-7947	VBN	denotes	used
T5688	7948-7950	IN	denotes	as
T5689	7951-7952	DT	denotes	a
T5690	7953-7960	NN	denotes	control
T5691	7960-7962	,	denotes	,
T5676	7962-7967	VBZ	denotes	binds
T5692	7968-7970	IN	denotes	at
T5693	7971-7974	DT	denotes	the
T5695	7975-7979	NN	denotes	fork
T5694	7980-7988	NN	denotes	junction
T5696	7989-7992	CC	denotes	and
T5697	7993-8000	VBZ	denotes	unwinds
T5698	8001-8004	DT	denotes	the
T5699	8005-8009	NN	denotes	fork
T5700	8010-8013	CC	denotes	and
T5701	8014-8017	DT	denotes	the
T5703	8018-8025	JJ	denotes	nascent
T5704	8026-8033	VBG	denotes	lagging
T5705	8034-8040	NN	denotes	strand
T5702	8041-8044	NN	denotes	DNA
T5706	8045-8047	IN	denotes	on
T5707	8048-8049	NN	denotes	A
T5709	8049-8050	HYPH	denotes	-
T5708	8050-8054	NN	denotes	fork
T5710	8054-8055	-LRB-	denotes	[
T5711	8055-8056	CD	denotes	5
T5712	8056-8057	SYM	denotes	′
T5713	8057-8058	-RRB-	denotes	]
T5714	8058-8060	,	denotes	,
T5715	8060-8062	CC	denotes	or
T5716	8063-8070	VBZ	denotes	unwinds
T5717	8071-8074	DT	denotes	the
T5719	8075-8082	JJ	denotes	nascent
T5720	8083-8090	VBG	denotes	lagging
T5718	8091-8097	NN	denotes	strand
T5721	8098-8100	IN	denotes	on
T5722	8101-8102	NN	denotes	A
T5724	8102-8103	HYPH	denotes	-
T5723	8103-8107	NN	denotes	fork
T5725	8107-8108	-LRB-	denotes	[
T5726	8108-8109	CD	denotes	3
T5727	8109-8110	SYM	denotes	′
T5728	8110-8111	,	denotes	,
T5729	8111-8112	CD	denotes	5
T5730	8112-8113	SYM	denotes	′
T5731	8113-8114	-RRB-	denotes	]
T5732	8114-8116	,	denotes	,
T5733	8116-8118	IN	denotes	as
T5734	8119-8127	VBN	denotes	reported
T5735	8128-8134	RB	denotes	before
T5736	8135-8136	-LRB-	denotes	(
T5737	8136-8138	CD	denotes	25
T5738	8138-8139	-RRB-	denotes	)
T5739	8139-8140	.	denotes	.
T5740	8140-8304	sentence	denotes	These results indicate that the Mcm4/6/7 complex binds to single-stranded DNA and that its helicase is activated only in the presence of a single-stranded 3′-tail.
T5741	8141-8146	DT	denotes	These
T5742	8147-8154	NNS	denotes	results
T5743	8155-8163	VBP	denotes	indicate
T5744	8164-8168	IN	denotes	that
T5746	8169-8172	DT	denotes	the
T5748	8173-8177	NN	denotes	Mcm4
T5749	8177-8178	HYPH	denotes	/
T5750	8178-8179	CD	denotes	6
T5751	8179-8180	HYPH	denotes	/
T5752	8180-8181	CD	denotes	7
T5747	8182-8189	NN	denotes	complex
T5745	8190-8195	VBZ	denotes	binds
T5753	8196-8198	IN	denotes	to
T5754	8199-8205	JJ	denotes	single
T5756	8205-8206	HYPH	denotes	-
T5755	8206-8214	VBN	denotes	stranded
T5757	8215-8218	NN	denotes	DNA
T5758	8219-8222	CC	denotes	and
T5759	8223-8227	IN	denotes	that
T5761	8228-8231	PRP$	denotes	its
T5762	8232-8240	NN	denotes	helicase
T5763	8241-8243	VBZ	denotes	is
T5760	8244-8253	VBN	denotes	activated
T5764	8254-8258	RB	denotes	only
T5765	8259-8261	IN	denotes	in
T5766	8262-8265	DT	denotes	the
T5767	8266-8274	NN	denotes	presence
T5768	8275-8277	IN	denotes	of
T5769	8278-8279	DT	denotes	a
T5771	8280-8286	JJ	denotes	single
T5773	8286-8287	HYPH	denotes	-
T5772	8287-8295	VBN	denotes	stranded
T5774	8296-8297	CD	denotes	3
T5775	8297-8298	SYM	denotes	′
T5776	8298-8299	HYPH	denotes	-
T5770	8299-8303	NN	denotes	tail
T5777	8303-8304	.	denotes	.
T5933	8306-8307	CD	denotes	3
T5935	8307-8308	SYM	denotes	′
T5936	8308-8309	HYPH	denotes	-
T5934	8309-8318	NN	denotes	extension
T5938	8319-8323	IN	denotes	with
T5939	8324-8328	NN	denotes	poly
T5941	8328-8329	-LRB-	denotes	(
T5940	8329-8331	NN	denotes	dT
T5943	8331-8332	-RRB-	denotes	)
T5942	8333-8337	NN	denotes	tail
T5944	8338-8340	VBZ	denotes	is
T5937	8341-8350	VBN	denotes	displaced
T5945	8351-8353	IN	denotes	by
T5946	8354-8358	NN	denotes	Mcm4
T5947	8358-8359	HYPH	denotes	/
T5948	8359-8360	CD	denotes	6
T5949	8360-8361	HYPH	denotes	/
T5950	8361-8362	CD	denotes	7
T5951	8362-8515	sentence	denotes	It has been reported that the 3′-extension can be unwound by the archaeal Mcm homohexamer but not by fission and budding yeast Mcm complexes (14,26,27).
T5952	8363-8365	PRP	denotes	It
T5954	8366-8369	VBZ	denotes	has
T5955	8370-8374	VBN	denotes	been
T5953	8375-8383	VBN	denotes	reported
T5956	8384-8388	IN	denotes	that
T5958	8389-8392	DT	denotes	the
T5960	8393-8394	CD	denotes	3
T5961	8394-8395	SYM	denotes	′
T5962	8395-8396	HYPH	denotes	-
T5959	8396-8405	NN	denotes	extension
T5963	8406-8409	MD	denotes	can
T5964	8410-8412	VB	denotes	be
T5957	8413-8420	JJ	denotes	unwound
T5965	8421-8423	IN	denotes	by
T5966	8424-8427	DT	denotes	the
T5968	8428-8436	JJ	denotes	archaeal
T5969	8437-8440	NN	denotes	Mcm
T5967	8441-8452	NN	denotes	homohexamer
T5970	8453-8456	CC	denotes	but
T5971	8457-8460	RB	denotes	not
T5972	8461-8463	IN	denotes	by
T5973	8464-8471	NN	denotes	fission
T5975	8472-8475	CC	denotes	and
T5976	8476-8483	NN	denotes	budding
T5977	8484-8489	NN	denotes	yeast
T5978	8490-8493	NN	denotes	Mcm
T5974	8494-8503	NNS	denotes	complexes
T5979	8504-8505	-LRB-	denotes	(
T5981	8505-8507	CD	denotes	14
T5982	8507-8508	,	denotes	,
T5983	8508-8510	CD	denotes	26
T5984	8510-8511	,	denotes	,
T5980	8511-8513	CD	denotes	27
T5985	8513-8514	-RRB-	denotes	)
T5986	8514-8515	.	denotes	.
T5987	8515-8695	sentence	denotes	We have constructed 3′-extension substrates containing poly(dT), poly(dA), poly(dC) and poly(dG) 3′-tails in order to examine whether mouse Mcm4/6/7 can displace these substrates.
T5988	8516-8518	PRP	denotes	We
T5990	8519-8523	VBP	denotes	have
T5989	8524-8535	VBN	denotes	constructed
T5991	8536-8537	CD	denotes	3
T5993	8537-8538	SYM	denotes	′
T5994	8538-8539	HYPH	denotes	-
T5992	8539-8548	NN	denotes	extension
T5995	8549-8559	NNS	denotes	substrates
T5996	8560-8570	VBG	denotes	containing
T5997	8571-8575	NN	denotes	poly
T5999	8575-8576	-LRB-	denotes	(
T5998	8576-8578	NN	denotes	dT
T6001	8578-8579	-RRB-	denotes	)
T6002	8579-8581	,	denotes	,
T6003	8581-8585	NN	denotes	poly
T6005	8585-8586	-LRB-	denotes	(
T6004	8586-8588	NN	denotes	dA
T6006	8588-8589	-RRB-	denotes	)
T6007	8589-8591	,	denotes	,
T6008	8591-8595	NN	denotes	poly
T6010	8595-8596	-LRB-	denotes	(
T6009	8596-8598	NN	denotes	dC
T6011	8598-8599	-RRB-	denotes	)
T6012	8600-8603	CC	denotes	and
T6013	8604-8608	NN	denotes	poly
T6015	8608-8609	-LRB-	denotes	(
T6014	8609-8611	NN	denotes	dG
T6016	8611-8612	-RRB-	denotes	)
T6017	8613-8614	CD	denotes	3
T6018	8614-8615	SYM	denotes	′
T6019	8615-8616	HYPH	denotes	-
T6000	8616-8621	NNS	denotes	tails
T6020	8622-8624	IN	denotes	in
T6021	8625-8630	NN	denotes	order
T6022	8631-8633	TO	denotes	to
T6023	8634-8641	VB	denotes	examine
T6024	8642-8649	IN	denotes	whether
T6026	8650-8655	NN	denotes	mouse
T6027	8656-8660	NN	denotes	Mcm4
T6028	8660-8661	HYPH	denotes	/
T6029	8661-8662	CD	denotes	6
T6030	8662-8663	HYPH	denotes	/
T6031	8663-8664	CD	denotes	7
T6032	8665-8668	MD	denotes	can
T6025	8669-8677	VB	denotes	displace
T6033	8678-8683	DT	denotes	these
T6034	8684-8694	NNS	denotes	substrates
T6035	8694-8695	.	denotes	.
T6036	8695-8846	sentence	denotes	We first found that Mcm4/6/7 binds to dT-, dC- and dG-3′-extension with similar affinity, and to dA-3′-extension with much lower affinity (Figure 5A).
T6037	8696-8698	PRP	denotes	We
T6039	8699-8704	RB	denotes	first
T6038	8705-8710	VBD	denotes	found
T6040	8711-8715	IN	denotes	that
T6042	8716-8720	NN	denotes	Mcm4
T6043	8720-8721	HYPH	denotes	/
T6044	8721-8722	CD	denotes	6
T6045	8722-8723	HYPH	denotes	/
T6046	8723-8724	CD	denotes	7
T6041	8725-8730	VBZ	denotes	binds
T6047	8731-8733	IN	denotes	to
T6048	8734-8736	NN	denotes	dT
T6050	8736-8737	HYPH	denotes	-
T6051	8737-8739	,	denotes	,
T6052	8739-8741	NN	denotes	dC
T6053	8741-8742	HYPH	denotes	-
T6054	8743-8746	CC	denotes	and
T6055	8747-8749	NN	denotes	dG
T6056	8749-8750	HYPH	denotes	-
T6057	8750-8751	CD	denotes	3
T6058	8751-8752	SYM	denotes	′
T6059	8752-8753	HYPH	denotes	-
T6049	8753-8762	NN	denotes	extension
T6060	8763-8767	IN	denotes	with
T6061	8768-8775	JJ	denotes	similar
T6062	8776-8784	NN	denotes	affinity
T6063	8784-8786	,	denotes	,
T6064	8786-8789	CC	denotes	and
T6065	8790-8792	IN	denotes	to
T6066	8793-8795	NN	denotes	dA
T6068	8795-8796	HYPH	denotes	-
T6069	8796-8797	CD	denotes	3
T6070	8797-8798	SYM	denotes	′
T6071	8798-8799	HYPH	denotes	-
T6067	8799-8808	NN	denotes	extension
T6072	8809-8813	IN	denotes	with
T6073	8814-8818	RB	denotes	much
T6074	8819-8824	JJR	denotes	lower
T6075	8825-8833	NN	denotes	affinity
T6076	8834-8835	-LRB-	denotes	(
T6078	8835-8841	NN	denotes	Figure
T6077	8842-8844	NN	denotes	5A
T6079	8844-8845	-RRB-	denotes	)
T6080	8845-8846	.	denotes	.
T6081	8846-8977	sentence	denotes	This is consistent with the previous results that Mcm4/6/7 binds to 50mer oligo-dT, -dC and -dG, but not to a 50mer oligo-dA (13).
T6082	8847-8851	DT	denotes	This
T6083	8852-8854	VBZ	denotes	is
T6084	8855-8865	JJ	denotes	consistent
T6085	8866-8870	IN	denotes	with
T6086	8871-8874	DT	denotes	the
T6088	8875-8883	JJ	denotes	previous
T6087	8884-8891	NNS	denotes	results
T6089	8892-8896	IN	denotes	that
T6091	8897-8901	NN	denotes	Mcm4
T6092	8901-8902	HYPH	denotes	/
T6093	8902-8903	CD	denotes	6
T6094	8903-8904	HYPH	denotes	/
T6095	8904-8905	CD	denotes	7
T6090	8906-8911	VBZ	denotes	binds
T6096	8912-8914	IN	denotes	to
T6097	8915-8920	NN	denotes	50mer
T6099	8921-8926	NN	denotes	oligo
T6100	8926-8927	HYPH	denotes	-
T6098	8927-8929	NN	denotes	dT
T6101	8929-8931	,	denotes	,
T6102	8931-8932	HYPH	denotes	-
T6103	8932-8934	NN	denotes	dC
T6104	8935-8938	CC	denotes	and
T6105	8939-8940	HYPH	denotes	-
T6106	8940-8942	NN	denotes	dG
T6107	8942-8944	,	denotes	,
T6108	8944-8947	CC	denotes	but
T6109	8948-8951	RB	denotes	not
T6110	8952-8954	IN	denotes	to
T6111	8955-8956	DT	denotes	a
T6113	8957-8962	NN	denotes	50mer
T6114	8963-8968	NN	denotes	oligo
T6115	8968-8969	HYPH	denotes	-
T6112	8969-8971	NN	denotes	dA
T6116	8972-8973	-LRB-	denotes	(
T6117	8973-8975	CD	denotes	13
T6118	8975-8976	-RRB-	denotes	)
T6119	8976-8977	.	denotes	.
T6120	8977-9145	sentence	denotes	We found that dT-tailed 3′-extension was displaced efficiently by Mcm 4/6/7 while almost no or very little activity was detected on other substrates (Figure 5B and C).
T6121	8978-8980	PRP	denotes	We
T6122	8981-8986	VBD	denotes	found
T6123	8987-8991	IN	denotes	that
T6125	8992-8994	NN	denotes	dT
T6127	8994-8995	HYPH	denotes	-
T6126	8995-9001	VBN	denotes	tailed
T6129	9002-9003	CD	denotes	3
T6130	9003-9004	SYM	denotes	′
T6131	9004-9005	HYPH	denotes	-
T6128	9005-9014	NN	denotes	extension
T6132	9015-9018	VBD	denotes	was
T6124	9019-9028	VBN	denotes	displaced
T6133	9029-9040	RB	denotes	efficiently
T6134	9041-9043	IN	denotes	by
T6135	9044-9047	NN	denotes	Mcm
T6136	9048-9049	CD	denotes	4
T6137	9049-9050	HYPH	denotes	/
T6138	9050-9051	CD	denotes	6
T6139	9051-9052	HYPH	denotes	/
T6140	9052-9053	CD	denotes	7
T6141	9054-9059	IN	denotes	while
T6143	9060-9066	RB	denotes	almost
T6144	9067-9069	DT	denotes	no
T6145	9070-9072	CC	denotes	or
T6146	9073-9077	RB	denotes	very
T6147	9078-9084	JJ	denotes	little
T6148	9085-9093	NN	denotes	activity
T6149	9094-9097	VBD	denotes	was
T6142	9098-9106	VBN	denotes	detected
T6150	9107-9109	IN	denotes	on
T6151	9110-9115	JJ	denotes	other
T6152	9116-9126	NNS	denotes	substrates
T6153	9127-9128	-LRB-	denotes	(
T6155	9128-9134	NN	denotes	Figure
T6154	9135-9137	NN	denotes	5B
T6156	9138-9141	CC	denotes	and
T6157	9142-9143	NN	denotes	C
T6158	9143-9144	-RRB-	denotes	)
T6159	9144-9145	.	denotes	.
T6160	9145-9259	sentence	denotes	This is in sharp contrast to yeast Mcm4/6/7 helicases which are not capable of unwinding 3′-extension substrates.
T6161	9146-9150	DT	denotes	This
T6162	9151-9153	VBZ	denotes	is
T6163	9154-9156	IN	denotes	in
T6164	9157-9162	JJ	denotes	sharp
T6165	9163-9171	NN	denotes	contrast
T6166	9172-9174	IN	denotes	to
T6167	9175-9180	NN	denotes	yeast
T6169	9181-9185	NN	denotes	Mcm4
T6170	9185-9186	HYPH	denotes	/
T6171	9186-9187	CD	denotes	6
T6172	9187-9188	HYPH	denotes	/
T6173	9188-9189	CD	denotes	7
T6168	9190-9199	NNS	denotes	helicases
T6174	9200-9205	WDT	denotes	which
T6175	9206-9209	VBP	denotes	are
T6176	9210-9213	RB	denotes	not
T6177	9214-9221	JJ	denotes	capable
T6178	9222-9224	IN	denotes	of
T6179	9225-9234	VBG	denotes	unwinding
T6180	9235-9236	CD	denotes	3
T6182	9236-9237	SYM	denotes	′
T6183	9237-9238	HYPH	denotes	-
T6181	9238-9247	NN	denotes	extension
T6184	9248-9258	NNS	denotes	substrates
T6185	9258-9259	.	denotes	.
T6186	9259-9365	sentence	denotes	Furthermore, dT-tailed 5′-extension, to which Mcm4/6/7 could bind, was not displaced by the Mcm helicase.
T6187	9260-9271	RB	denotes	Furthermore
T6189	9271-9273	,	denotes	,
T6190	9273-9275	NN	denotes	dT
T6192	9275-9276	HYPH	denotes	-
T6191	9276-9282	VBN	denotes	tailed
T6194	9283-9284	CD	denotes	5
T6195	9284-9285	SYM	denotes	′
T6196	9285-9286	HYPH	denotes	-
T6193	9286-9295	NN	denotes	extension
T6197	9295-9297	,	denotes	,
T6198	9297-9299	IN	denotes	to
T6200	9300-9305	WDT	denotes	which
T6201	9306-9310	NN	denotes	Mcm4
T6202	9310-9311	HYPH	denotes	/
T6203	9311-9312	CD	denotes	6
T6204	9312-9313	HYPH	denotes	/
T6205	9313-9314	CD	denotes	7
T6206	9315-9320	MD	denotes	could
T6199	9321-9325	VB	denotes	bind
T6207	9325-9327	,	denotes	,
T6208	9327-9330	VBD	denotes	was
T6209	9331-9334	RB	denotes	not
T6188	9335-9344	VBN	denotes	displaced
T6210	9345-9347	IN	denotes	by
T6211	9348-9351	DT	denotes	the
T6213	9352-9355	NN	denotes	Mcm
T6212	9356-9364	NN	denotes	helicase
T6214	9364-9365	.	denotes	.
T6215	9365-9556	sentence	denotes	The addition of the 5′-tail to dT-tailed 3′-extension further stimulated the displacement (Figure 5D), confirming the previous report that the fork structures facilitate the helicase action.
T6216	9366-9369	DT	denotes	The
T6217	9370-9378	NN	denotes	addition
T6219	9379-9381	IN	denotes	of
T6220	9382-9385	DT	denotes	the
T6222	9386-9387	CD	denotes	5
T6223	9387-9388	SYM	denotes	′
T6224	9388-9389	HYPH	denotes	-
T6221	9389-9393	NN	denotes	tail
T6225	9394-9396	IN	denotes	to
T6226	9397-9399	NN	denotes	dT
T6228	9399-9400	HYPH	denotes	-
T6227	9400-9406	VBN	denotes	tailed
T6230	9407-9408	CD	denotes	3
T6231	9408-9409	SYM	denotes	′
T6232	9409-9410	HYPH	denotes	-
T6229	9410-9419	NN	denotes	extension
T6233	9420-9427	RB	denotes	further
T6218	9428-9438	VBD	denotes	stimulated
T6234	9439-9442	DT	denotes	the
T6235	9443-9455	NN	denotes	displacement
T6236	9456-9457	-LRB-	denotes	(
T6238	9457-9463	NN	denotes	Figure
T6237	9464-9466	NN	denotes	5D
T6239	9466-9467	-RRB-	denotes	)
T6240	9467-9469	,	denotes	,
T6241	9469-9479	VBG	denotes	confirming
T6242	9480-9483	DT	denotes	the
T6244	9484-9492	JJ	denotes	previous
T6243	9493-9499	NN	denotes	report
T6245	9500-9504	IN	denotes	that
T6247	9505-9508	DT	denotes	the
T6249	9509-9513	NN	denotes	fork
T6248	9514-9524	NNS	denotes	structures
T6246	9525-9535	VBP	denotes	facilitate
T6250	9536-9539	DT	denotes	the
T6252	9540-9548	NN	denotes	helicase
T6251	9549-9555	NN	denotes	action
T6253	9555-9556	.	denotes	.
T6254	9556-9720	sentence	denotes	Thus, these results further strengthen our conclusion that the mammalian Mcm helicase is generally activated by the thymine-stretch on the 3′-single-stranded tail.
T6255	9557-9561	RB	denotes	Thus
T6257	9561-9563	,	denotes	,
T6258	9563-9568	DT	denotes	these
T6259	9569-9576	NNS	denotes	results
T6260	9577-9584	RB	denotes	further
T6256	9585-9595	VB	denotes	strengthen
T6261	9596-9599	PRP$	denotes	our
T6262	9600-9610	NN	denotes	conclusion
T6263	9611-9615	IN	denotes	that
T6265	9616-9619	DT	denotes	the
T6267	9620-9629	JJ	denotes	mammalian
T6268	9630-9633	NN	denotes	Mcm
T6266	9634-9642	NN	denotes	helicase
T6269	9643-9645	VBZ	denotes	is
T6270	9646-9655	RB	denotes	generally
T6264	9656-9665	VBN	denotes	activated
T6271	9666-9668	IN	denotes	by
T6272	9669-9672	DT	denotes	the
T6274	9673-9680	NN	denotes	thymine
T6275	9680-9681	HYPH	denotes	-
T6273	9681-9688	NN	denotes	stretch
T6276	9689-9691	IN	denotes	on
T6277	9692-9695	DT	denotes	the
T6279	9696-9697	CD	denotes	3
T6280	9697-9698	SYM	denotes	′
T6281	9698-9699	HYPH	denotes	-
T6282	9699-9705	JJ	denotes	single
T6284	9705-9706	HYPH	denotes	-
T6283	9706-9714	VBN	denotes	stranded
T6278	9715-9719	NN	denotes	tail
T6285	9719-9720	.	denotes	.
T6404	9722-9725	DT	denotes	The
T6405	9726-9733	NNS	denotes	effects
T6406	9734-9736	IN	denotes	of
T6407	9737-9740	DT	denotes	the
T6409	9741-9748	NN	denotes	thymine
T6408	9749-9756	NN	denotes	content
T6410	9757-9759	IN	denotes	of
T6411	9760-9763	DT	denotes	the
T6413	9764-9765	CD	denotes	3
T6414	9765-9766	SYM	denotes	′
T6415	9766-9767	HYPH	denotes	-
T6412	9767-9771	NN	denotes	tail
T6416	9772-9774	IN	denotes	on
T6417	9775-9778	NN	denotes	Mcm
T6418	9779-9787	NN	denotes	helicase
T6419	9788-9796	NN	denotes	activity
T6420	9796-9933	sentence	denotes	We next examined more systematically the effect of the compositions of the thymine residues of the 3′-tail on the Mcm helicase activity.
T6421	9797-9799	PRP	denotes	We
T6423	9800-9804	RB	denotes	next
T6422	9805-9813	VBD	denotes	examined
T6424	9814-9818	RBR	denotes	more
T6425	9819-9833	RB	denotes	systematically
T6426	9834-9837	DT	denotes	the
T6427	9838-9844	NN	denotes	effect
T6428	9845-9847	IN	denotes	of
T6429	9848-9851	DT	denotes	the
T6430	9852-9864	NNS	denotes	compositions
T6431	9865-9867	IN	denotes	of
T6432	9868-9871	DT	denotes	the
T6434	9872-9879	NN	denotes	thymine
T6433	9880-9888	NNS	denotes	residues
T6435	9889-9891	IN	denotes	of
T6436	9892-9895	DT	denotes	the
T6438	9896-9897	CD	denotes	3
T6439	9897-9898	SYM	denotes	′
T6440	9898-9899	HYPH	denotes	-
T6437	9899-9903	NN	denotes	tail
T6441	9904-9906	IN	denotes	on
T6442	9907-9910	DT	denotes	the
T6444	9911-9914	NN	denotes	Mcm
T6445	9915-9923	NN	denotes	helicase
T6443	9924-9932	NN	denotes	activity
T6446	9932-9933	.	denotes	.
T6447	9933-10180	sentence	denotes	A partial heteroduplex M13 single-stranded DNA containing a 37 bp duplex region in the presence or absence of the 50 nt long 3′-tail of various nucleotide compositions were constructed and the unwinding by mouse Mcm4/6/7 was examined (Figure 6A).
T6448	9934-9935	DT	denotes	A
T6450	9936-9943	JJ	denotes	partial
T6451	9944-9956	NN	denotes	heteroduplex
T6452	9957-9960	NN	denotes	M13
T6453	9961-9967	JJ	denotes	single
T6455	9967-9968	HYPH	denotes	-
T6454	9968-9976	VBN	denotes	stranded
T6449	9977-9980	NN	denotes	DNA
T6457	9981-9991	VBG	denotes	containing
T6458	9992-9993	DT	denotes	a
T6460	9994-9996	CD	denotes	37
T6461	9997-9999	NN	denotes	bp
T6462	10000-10006	NN	denotes	duplex
T6459	10007-10013	NN	denotes	region
T6463	10014-10016	IN	denotes	in
T6464	10017-10020	DT	denotes	the
T6465	10021-10029	NN	denotes	presence
T6466	10030-10032	CC	denotes	or
T6467	10033-10040	NN	denotes	absence
T6468	10041-10043	IN	denotes	of
T6469	10044-10047	DT	denotes	the
T6471	10048-10050	CD	denotes	50
T6472	10051-10053	NN	denotes	nt
T6473	10054-10058	JJ	denotes	long
T6474	10059-10060	CD	denotes	3
T6475	10060-10061	SYM	denotes	′
T6476	10061-10062	HYPH	denotes	-
T6470	10062-10066	NN	denotes	tail
T6477	10067-10069	IN	denotes	of
T6478	10070-10077	JJ	denotes	various
T6480	10078-10088	NN	denotes	nucleotide
T6479	10089-10101	NNS	denotes	compositions
T6481	10102-10106	VBD	denotes	were
T6456	10107-10118	VBN	denotes	constructed
T6482	10119-10122	CC	denotes	and
T6483	10123-10126	DT	denotes	the
T6484	10127-10136	NN	denotes	unwinding
T6486	10137-10139	IN	denotes	by
T6487	10140-10145	NN	denotes	mouse
T6488	10146-10150	NN	denotes	Mcm4
T6489	10150-10151	HYPH	denotes	/
T6490	10151-10152	CD	denotes	6
T6491	10152-10153	HYPH	denotes	/
T6492	10153-10154	CD	denotes	7
T6493	10155-10158	VBD	denotes	was
T6485	10159-10167	VBN	denotes	examined
T6494	10168-10169	-LRB-	denotes	(
T6496	10169-10175	NN	denotes	Figure
T6495	10176-10178	NN	denotes	6A
T6497	10178-10179	-RRB-	denotes	)
T6498	10179-10180	.	denotes	.
T6499	10180-10308	sentence	denotes	Due to its low processivity, Mcm4/6/7 barely displaced the annealed 37mer oligonucleotide without a 3′-tail (37mer; Figure 6A).
T6500	10181-10184	IN	denotes	Due
T6502	10185-10187	IN	denotes	to
T6503	10188-10191	PRP$	denotes	its
T6505	10192-10195	JJ	denotes	low
T6504	10196-10208	NN	denotes	processivity
T6506	10208-10210	,	denotes	,
T6507	10210-10214	NN	denotes	Mcm4
T6508	10214-10215	HYPH	denotes	/
T6509	10215-10216	CD	denotes	6
T6510	10216-10217	HYPH	denotes	/
T6511	10217-10218	CD	denotes	7
T6512	10219-10225	RB	denotes	barely
T6501	10226-10235	VBD	denotes	displaced
T6513	10236-10239	DT	denotes	the
T6515	10240-10248	JJ	denotes	annealed
T6516	10249-10254	NN	denotes	37mer
T6514	10255-10270	NN	denotes	oligonucleotide
T6517	10271-10278	IN	denotes	without
T6518	10279-10280	DT	denotes	a
T6520	10281-10282	CD	denotes	3
T6521	10282-10283	SYM	denotes	′
T6522	10283-10284	HYPH	denotes	-
T6519	10284-10288	NN	denotes	tail
T6523	10289-10290	-LRB-	denotes	(
T6525	10290-10295	NN	denotes	37mer
T6526	10295-10296	:	denotes	;
T6527	10297-10303	NN	denotes	Figure
T6524	10304-10306	NN	denotes	6A
T6528	10306-10307	-RRB-	denotes	)
T6529	10307-10308	.	denotes	.
T6530	10308-10501	sentence	denotes	Only weak helicase activity was observed on the substrate carrying the 3′-tail of 25 repeats of TA dinucleotide (37-TA25; Figure 6A), whereas strong helicase activity was observed on 37-TTA17.
T6531	10309-10313	RB	denotes	Only
T6533	10314-10318	JJ	denotes	weak
T6534	10319-10327	NN	denotes	helicase
T6532	10328-10336	NN	denotes	activity
T6536	10337-10340	VBD	denotes	was
T6535	10341-10349	VBN	denotes	observed
T6537	10350-10352	IN	denotes	on
T6538	10353-10356	DT	denotes	the
T6539	10357-10366	NN	denotes	substrate
T6540	10367-10375	VBG	denotes	carrying
T6541	10376-10379	DT	denotes	the
T6543	10380-10381	CD	denotes	3
T6544	10381-10382	SYM	denotes	′
T6545	10382-10383	HYPH	denotes	-
T6542	10383-10387	NN	denotes	tail
T6546	10388-10390	IN	denotes	of
T6547	10391-10393	CD	denotes	25
T6548	10394-10401	NNS	denotes	repeats
T6549	10402-10404	IN	denotes	of
T6550	10405-10407	NN	denotes	TA
T6551	10408-10420	NN	denotes	dinucleotide
T6552	10421-10422	-LRB-	denotes	(
T6554	10422-10424	CD	denotes	37
T6556	10424-10425	HYPH	denotes	-
T6555	10425-10429	NN	denotes	TA25
T6557	10429-10430	:	denotes	;
T6558	10431-10437	NN	denotes	Figure
T6553	10438-10440	NN	denotes	6A
T6559	10440-10441	-RRB-	denotes	)
T6560	10441-10443	,	denotes	,
T6561	10443-10450	IN	denotes	whereas
T6563	10451-10457	JJ	denotes	strong
T6565	10458-10466	NN	denotes	helicase
T6564	10467-10475	NN	denotes	activity
T6566	10476-10479	VBD	denotes	was
T6562	10480-10488	VBN	denotes	observed
T6567	10489-10491	IN	denotes	on
T6568	10492-10494	CD	denotes	37
T6570	10494-10495	HYPH	denotes	-
T6569	10495-10500	NN	denotes	TTA17
T6571	10500-10501	.	denotes	.
T6572	10501-10751	sentence	denotes	Increase of thymine content on the 3′-tail did not further increase the extent of unwinding (37-TTTA13, 37-TTTTA10 and 37-T50; Figure 6A), suggesting that 17 repeats of TT dinucleotides (67% thymine) may be sufficient for activation of Mcm helicase.
T6573	10502-10510	NN	denotes	Increase
T6575	10511-10513	IN	denotes	of
T6576	10514-10521	NN	denotes	thymine
T6577	10522-10529	NN	denotes	content
T6578	10530-10532	IN	denotes	on
T6579	10533-10536	DT	denotes	the
T6581	10537-10538	CD	denotes	3
T6582	10538-10539	SYM	denotes	′
T6583	10539-10540	HYPH	denotes	-
T6580	10540-10544	NN	denotes	tail
T6584	10545-10548	VBD	denotes	did
T6585	10549-10552	RB	denotes	not
T6586	10553-10560	RB	denotes	further
T6574	10561-10569	VB	denotes	increase
T6587	10570-10573	DT	denotes	the
T6588	10574-10580	NN	denotes	extent
T6589	10581-10583	IN	denotes	of
T6590	10584-10593	NN	denotes	unwinding
T6591	10594-10595	-LRB-	denotes	(
T6593	10595-10597	CD	denotes	37
T6595	10597-10598	HYPH	denotes	-
T6594	10598-10604	NN	denotes	TTTA13
T6596	10604-10606	,	denotes	,
T6597	10606-10608	CD	denotes	37
T6599	10608-10609	HYPH	denotes	-
T6598	10609-10616	NN	denotes	TTTTA10
T6600	10617-10620	CC	denotes	and
T6601	10621-10623	CD	denotes	37
T6603	10623-10624	HYPH	denotes	-
T6602	10624-10627	NN	denotes	T50
T6604	10627-10628	:	denotes	;
T6605	10629-10635	NN	denotes	Figure
T6592	10636-10638	NN	denotes	6A
T6606	10638-10639	-RRB-	denotes	)
T6607	10639-10641	,	denotes	,
T6608	10641-10651	VBG	denotes	suggesting
T6609	10652-10656	IN	denotes	that
T6611	10657-10659	CD	denotes	17
T6612	10660-10667	NNS	denotes	repeats
T6613	10668-10670	IN	denotes	of
T6614	10671-10673	NN	denotes	TT
T6615	10674-10687	NNS	denotes	dinucleotides
T6616	10688-10689	-LRB-	denotes	(
T6618	10689-10691	CD	denotes	67
T6619	10691-10692	NN	denotes	%
T6617	10693-10700	NN	denotes	thymine
T6620	10700-10701	-RRB-	denotes	)
T6621	10702-10705	MD	denotes	may
T6610	10706-10708	VB	denotes	be
T6622	10709-10719	JJ	denotes	sufficient
T6623	10720-10723	IN	denotes	for
T6624	10724-10734	NN	denotes	activation
T6625	10735-10737	IN	denotes	of
T6626	10738-10741	NN	denotes	Mcm
T6627	10742-10750	NN	denotes	helicase
T6628	10750-10751	.	denotes	.
T6629	10751-10952	sentence	denotes	It should be noted that the TA25 tail would self-anneal and this would prevent the loading of Mcm, since the 3′-tail containing TTAA13 or TTTAAA8 did not enhance the unwinding either (data not shown).
T6630	10752-10754	PRP	denotes	It
T6632	10755-10761	MD	denotes	should
T6633	10762-10764	VB	denotes	be
T6631	10765-10770	VBN	denotes	noted
T6634	10771-10775	IN	denotes	that
T6636	10776-10779	DT	denotes	the
T6638	10780-10784	NN	denotes	TA25
T6637	10785-10789	NN	denotes	tail
T6639	10790-10795	MD	denotes	would
T6640	10796-10800	NN	denotes	self
T6641	10800-10801	HYPH	denotes	-
T6635	10801-10807	VB	denotes	anneal
T6642	10808-10811	CC	denotes	and
T6643	10812-10816	DT	denotes	this
T6645	10817-10822	MD	denotes	would
T6644	10823-10830	VB	denotes	prevent
T6646	10831-10834	DT	denotes	the
T6647	10835-10842	NN	denotes	loading
T6648	10843-10845	IN	denotes	of
T6649	10846-10849	NN	denotes	Mcm
T6650	10849-10851	,	denotes	,
T6651	10851-10856	IN	denotes	since
T6653	10857-10860	DT	denotes	the
T6655	10861-10862	CD	denotes	3
T6656	10862-10863	SYM	denotes	′
T6657	10863-10864	HYPH	denotes	-
T6654	10864-10868	NN	denotes	tail
T6658	10869-10879	VBG	denotes	containing
T6659	10880-10886	NN	denotes	TTAA13
T6660	10887-10889	CC	denotes	or
T6661	10890-10897	NN	denotes	TTTAAA8
T6662	10898-10901	VBD	denotes	did
T6663	10902-10905	RB	denotes	not
T6652	10906-10913	VB	denotes	enhance
T6664	10914-10917	DT	denotes	the
T6665	10918-10927	NN	denotes	unwinding
T6666	10928-10934	RB	denotes	either
T6667	10935-10936	-LRB-	denotes	(
T6669	10936-10940	NNS	denotes	data
T6670	10941-10944	RB	denotes	not
T6668	10945-10950	VBN	denotes	shown
T6671	10950-10951	-RRB-	denotes	)
T6672	10951-10952	.	denotes	.
T6673	10952-11052	sentence	denotes	Therefore, we next examined the effects of repeating sequences of thymine and cytosine (Figure 6B).
T6674	10953-10962	RB	denotes	Therefore
T6676	10962-10964	,	denotes	,
T6677	10964-10966	PRP	denotes	we
T6678	10967-10971	RB	denotes	next
T6675	10972-10980	VBD	denotes	examined
T6679	10981-10984	DT	denotes	the
T6680	10985-10992	NNS	denotes	effects
T6681	10993-10995	IN	denotes	of
T6682	10996-11005	VBG	denotes	repeating
T6683	11006-11015	NNS	denotes	sequences
T6684	11016-11018	IN	denotes	of
T6685	11019-11026	NN	denotes	thymine
T6686	11027-11030	CC	denotes	and
T6687	11031-11039	NN	denotes	cytosine
T6688	11040-11041	-LRB-	denotes	(
T6690	11041-11047	NN	denotes	Figure
T6689	11048-11050	NN	denotes	6B
T6691	11050-11051	-RRB-	denotes	)
T6692	11051-11052	.	denotes	.
T6693	11052-11326	sentence	denotes	Partial heteroduplex substrates containing the 3′-tail of TC25, TTCC13, TTTCCC8, TTTTCCCC6, TTCC17 were displaced by the Mcm4/6/7 complex to the extent similar to that achieved by TTA17, suggesting that 50% thymine content is sufficient for full activation of Mcm helicase.
T6694	11053-11060	JJ	denotes	Partial
T6696	11061-11073	NN	denotes	heteroduplex
T6695	11074-11084	NNS	denotes	substrates
T6698	11085-11095	VBG	denotes	containing
T6699	11096-11099	DT	denotes	the
T6701	11100-11101	CD	denotes	3
T6702	11101-11102	SYM	denotes	′
T6703	11102-11103	HYPH	denotes	-
T6700	11103-11107	NN	denotes	tail
T6704	11108-11110	IN	denotes	of
T6705	11111-11115	NN	denotes	TC25
T6707	11115-11117	,	denotes	,
T6708	11117-11123	NN	denotes	TTCC13
T6709	11123-11125	,	denotes	,
T6710	11125-11132	NN	denotes	TTTCCC8
T6711	11132-11134	,	denotes	,
T6712	11134-11143	NN	denotes	TTTTCCCC6
T6713	11143-11145	,	denotes	,
T6706	11145-11151	NN	denotes	TTCC17
T6714	11152-11156	VBD	denotes	were
T6697	11157-11166	VBN	denotes	displaced
T6715	11167-11169	IN	denotes	by
T6716	11170-11173	DT	denotes	the
T6718	11174-11178	NN	denotes	Mcm4
T6719	11178-11179	HYPH	denotes	/
T6720	11179-11180	CD	denotes	6
T6721	11180-11181	HYPH	denotes	/
T6722	11181-11182	CD	denotes	7
T6717	11183-11190	NN	denotes	complex
T6723	11191-11193	IN	denotes	to
T6724	11194-11197	DT	denotes	the
T6725	11198-11204	NN	denotes	extent
T6726	11205-11212	JJ	denotes	similar
T6727	11213-11215	IN	denotes	to
T6728	11216-11220	DT	denotes	that
T6729	11221-11229	VBN	denotes	achieved
T6730	11230-11232	IN	denotes	by
T6731	11233-11238	NN	denotes	TTA17
T6732	11238-11240	,	denotes	,
T6733	11240-11250	VBG	denotes	suggesting
T6734	11251-11255	IN	denotes	that
T6736	11256-11258	CD	denotes	50
T6737	11258-11259	NN	denotes	%
T6739	11260-11267	NN	denotes	thymine
T6738	11268-11275	NN	denotes	content
T6735	11276-11278	VBZ	denotes	is
T6740	11279-11289	JJ	denotes	sufficient
T6741	11290-11293	IN	denotes	for
T6742	11294-11298	JJ	denotes	full
T6743	11299-11309	NN	denotes	activation
T6744	11310-11312	IN	denotes	of
T6745	11313-11316	NN	denotes	Mcm
T6746	11317-11325	NN	denotes	helicase
T6747	11325-11326	.	denotes	.
T6748	11326-11468	sentence	denotes	TTCC13 and TTCC17 were displaced to a similar extent, consistent with the earlier result that a 40 nt tail is sufficient for activation (13).
T6749	11327-11333	NN	denotes	TTCC13
T6751	11334-11337	CC	denotes	and
T6752	11338-11344	NN	denotes	TTCC17
T6753	11345-11349	VBD	denotes	were
T6750	11350-11359	VBN	denotes	displaced
T6754	11360-11362	IN	denotes	to
T6755	11363-11364	DT	denotes	a
T6757	11365-11372	JJ	denotes	similar
T6756	11373-11379	NN	denotes	extent
T6758	11379-11381	,	denotes	,
T6759	11381-11391	JJ	denotes	consistent
T6760	11392-11396	IN	denotes	with
T6761	11397-11400	DT	denotes	the
T6763	11401-11408	JJR	denotes	earlier
T6762	11409-11415	NN	denotes	result
T6764	11416-11420	IN	denotes	that
T6766	11421-11422	DT	denotes	a
T6768	11423-11425	CD	denotes	40
T6769	11426-11428	NN	denotes	nt
T6767	11429-11433	NN	denotes	tail
T6765	11434-11436	VBZ	denotes	is
T6770	11437-11447	JJ	denotes	sufficient
T6771	11448-11451	IN	denotes	for
T6772	11452-11462	NN	denotes	activation
T6773	11463-11464	-LRB-	denotes	(
T6774	11464-11466	CD	denotes	13
T6775	11466-11467	-RRB-	denotes	)
T6776	11467-11468	.	denotes	.
T6777	11468-11630	sentence	denotes	However, the efficiency of the displacement significantly decreased as the thymine content of the 3′-tail dropped to 33%, as in TAA17 or TCC17 (Figure 6B and C).
T6778	11469-11476	RB	denotes	However
T6780	11476-11478	,	denotes	,
T6781	11478-11481	DT	denotes	the
T6782	11482-11492	NN	denotes	efficiency
T6783	11493-11495	IN	denotes	of
T6784	11496-11499	DT	denotes	the
T6785	11500-11512	NN	denotes	displacement
T6786	11513-11526	RB	denotes	significantly
T6779	11527-11536	VBD	denotes	decreased
T6787	11537-11539	IN	denotes	as
T6789	11540-11543	DT	denotes	the
T6791	11544-11551	NN	denotes	thymine
T6790	11552-11559	NN	denotes	content
T6792	11560-11562	IN	denotes	of
T6793	11563-11566	DT	denotes	the
T6795	11567-11568	CD	denotes	3
T6796	11568-11569	SYM	denotes	′
T6797	11569-11570	HYPH	denotes	-
T6794	11570-11574	NN	denotes	tail
T6788	11575-11582	VBD	denotes	dropped
T6798	11583-11585	IN	denotes	to
T6799	11586-11588	CD	denotes	33
T6800	11588-11589	NN	denotes	%
T6801	11589-11591	,	denotes	,
T6802	11591-11593	IN	denotes	as
T6803	11594-11596	IN	denotes	in
T6804	11597-11602	NN	denotes	TAA17
T6805	11603-11605	CC	denotes	or
T6806	11606-11611	NN	denotes	TCC17
T6807	11612-11613	-LRB-	denotes	(
T6809	11613-11619	NN	denotes	Figure
T6808	11620-11622	NN	denotes	6B
T6810	11623-11626	CC	denotes	and
T6811	11627-11628	NN	denotes	C
T6812	11628-11629	-RRB-	denotes	)
T6813	11629-11630	.	denotes	.
T6814	11630-11745	sentence	denotes	Thus, the minimal thymine content required for activation of the Mcm helicase may be somewhere between 33 and 50%.
T6815	11631-11635	RB	denotes	Thus
T6817	11635-11637	,	denotes	,
T6818	11637-11640	DT	denotes	the
T6820	11641-11648	JJ	denotes	minimal
T6821	11649-11656	NN	denotes	thymine
T6819	11657-11664	NN	denotes	content
T6822	11665-11673	VBN	denotes	required
T6823	11674-11677	IN	denotes	for
T6824	11678-11688	NN	denotes	activation
T6825	11689-11691	IN	denotes	of
T6826	11692-11695	DT	denotes	the
T6828	11696-11699	NN	denotes	Mcm
T6827	11700-11708	NN	denotes	helicase
T6829	11709-11712	MD	denotes	may
T6816	11713-11715	VB	denotes	be
T6830	11716-11725	RB	denotes	somewhere
T6831	11726-11733	IN	denotes	between
T6832	11734-11736	CD	denotes	33
T6834	11737-11740	CC	denotes	and
T6835	11741-11743	CD	denotes	50
T6833	11743-11744	NN	denotes	%
T6836	11744-11745	.	denotes	.
T7008	11747-11750	NN	denotes	DUE
T7009	11751-11760	NNS	denotes	sequences
T7011	11761-11765	IN	denotes	from
T7012	11766-11769	DT	denotes	the
T7014	11770-11771	NN	denotes	c
T7016	11771-11772	HYPH	denotes	-
T7015	11772-11775	NN	denotes	myc
T7013	11776-11782	NN	denotes	origin
T7010	11783-11791	VBP	denotes	activate
T7017	11792-11795	NN	denotes	Mcm
T7018	11796-11804	NN	denotes	helicase
T7019	11804-12104	sentence	denotes	Our previous studies have shown that the sequences containing periodic (dT)n tracts derived from the human lamin B2 origin (48% thymine content) can serve as an efficient activator for the Mcm helicase on a bubble, and replacement of thymines with guanines abolished the helicase activation (13,28).
T7020	11805-11808	PRP$	denotes	Our
T7022	11809-11817	JJ	denotes	previous
T7021	11818-11825	NNS	denotes	studies
T7024	11826-11830	VBP	denotes	have
T7023	11831-11836	VBN	denotes	shown
T7025	11837-11841	IN	denotes	that
T7027	11842-11845	DT	denotes	the
T7028	11846-11855	NNS	denotes	sequences
T7029	11856-11866	VBG	denotes	containing
T7030	11867-11875	JJ	denotes	periodic
T7032	11876-11877	-LRB-	denotes	(
T7034	11877-11879	NN	denotes	dT
T7035	11879-11880	-RRB-	denotes	)
T7033	11880-11881	NN	denotes	n
T7031	11882-11888	NNS	denotes	tracts
T7036	11889-11896	VBN	denotes	derived
T7037	11897-11901	IN	denotes	from
T7038	11902-11905	DT	denotes	the
T7040	11906-11911	JJ	denotes	human
T7041	11912-11917	NN	denotes	lamin
T7042	11918-11920	NN	denotes	B2
T7039	11921-11927	NN	denotes	origin
T7043	11928-11929	-LRB-	denotes	(
T7045	11929-11931	CD	denotes	48
T7046	11931-11932	NN	denotes	%
T7047	11933-11940	NN	denotes	thymine
T7044	11941-11948	NN	denotes	content
T7048	11948-11949	-RRB-	denotes	)
T7049	11950-11953	MD	denotes	can
T7026	11954-11959	VB	denotes	serve
T7050	11960-11962	IN	denotes	as
T7051	11963-11965	DT	denotes	an
T7053	11966-11975	JJ	denotes	efficient
T7052	11976-11985	NN	denotes	activator
T7054	11986-11989	IN	denotes	for
T7055	11990-11993	DT	denotes	the
T7057	11994-11997	NN	denotes	Mcm
T7056	11998-12006	NN	denotes	helicase
T7058	12007-12009	IN	denotes	on
T7059	12010-12011	DT	denotes	a
T7060	12012-12018	NN	denotes	bubble
T7061	12018-12020	,	denotes	,
T7062	12020-12023	CC	denotes	and
T7063	12024-12035	NN	denotes	replacement
T7065	12036-12038	IN	denotes	of
T7066	12039-12047	NNS	denotes	thymines
T7067	12048-12052	IN	denotes	with
T7068	12053-12061	NNS	denotes	guanines
T7064	12062-12071	VBD	denotes	abolished
T7069	12072-12075	DT	denotes	the
T7071	12076-12084	NN	denotes	helicase
T7070	12085-12095	NN	denotes	activation
T7072	12096-12097	-LRB-	denotes	(
T7074	12097-12099	CD	denotes	13
T7075	12099-12100	,	denotes	,
T7073	12100-12102	CD	denotes	28
T7076	12102-12103	-RRB-	denotes	)
T7077	12103-12104	.	denotes	.
T7078	12104-12462	sentence	denotes	In order to examine other natural replication origins with different thymine contents for the ability to activate Mcm helicase on a bubble substrate, we constructed new bubble substrates, Bub82/c-myc, containing sequences derived from the DUE (DNA unwinding element) region of the human c-myc origin which is essential for c-myc replicator activity (29,30).
T7079	12105-12107	IN	denotes	In
T7081	12108-12113	NN	denotes	order
T7082	12114-12116	TO	denotes	to
T7083	12117-12124	VB	denotes	examine
T7084	12125-12130	JJ	denotes	other
T7086	12131-12138	JJ	denotes	natural
T7087	12139-12150	NN	denotes	replication
T7085	12151-12158	NNS	denotes	origins
T7088	12159-12163	IN	denotes	with
T7089	12164-12173	JJ	denotes	different
T7091	12174-12181	NN	denotes	thymine
T7090	12182-12190	NNS	denotes	contents
T7092	12191-12194	IN	denotes	for
T7093	12195-12198	DT	denotes	the
T7094	12199-12206	NN	denotes	ability
T7095	12207-12209	TO	denotes	to
T7096	12210-12218	VB	denotes	activate
T7097	12219-12222	NN	denotes	Mcm
T7098	12223-12231	NN	denotes	helicase
T7099	12232-12234	IN	denotes	on
T7100	12235-12236	DT	denotes	a
T7102	12237-12243	NN	denotes	bubble
T7101	12244-12253	NN	denotes	substrate
T7103	12253-12255	,	denotes	,
T7104	12255-12257	PRP	denotes	we
T7080	12258-12269	VBD	denotes	constructed
T7105	12270-12273	JJ	denotes	new
T7107	12274-12280	NN	denotes	bubble
T7106	12281-12291	NNS	denotes	substrates
T7108	12291-12293	,	denotes	,
T7109	12293-12298	NN	denotes	Bub82
T7111	12298-12299	HYPH	denotes	/
T7112	12299-12300	NN	denotes	c
T7113	12300-12301	HYPH	denotes	-
T7110	12301-12304	NN	denotes	myc
T7114	12304-12306	,	denotes	,
T7115	12306-12316	VBG	denotes	containing
T7116	12317-12326	NNS	denotes	sequences
T7117	12327-12334	VBN	denotes	derived
T7118	12335-12339	IN	denotes	from
T7119	12340-12343	DT	denotes	the
T7121	12344-12347	NN	denotes	DUE
T7122	12348-12349	-LRB-	denotes	(
T7123	12349-12352	NN	denotes	DNA
T7125	12353-12362	NN	denotes	unwinding
T7124	12363-12370	NN	denotes	element
T7126	12370-12371	-RRB-	denotes	)
T7120	12372-12378	NN	denotes	region
T7127	12379-12381	IN	denotes	of
T7128	12382-12385	DT	denotes	the
T7130	12386-12391	JJ	denotes	human
T7131	12392-12393	NN	denotes	c
T7133	12393-12394	HYPH	denotes	-
T7132	12394-12397	NN	denotes	myc
T7129	12398-12404	NN	denotes	origin
T7134	12405-12410	WDT	denotes	which
T7135	12411-12413	VBZ	denotes	is
T7136	12414-12423	JJ	denotes	essential
T7137	12424-12427	IN	denotes	for
T7138	12428-12429	NN	denotes	c
T7140	12429-12430	HYPH	denotes	-
T7139	12430-12433	NN	denotes	myc
T7142	12434-12444	NN	denotes	replicator
T7141	12445-12453	NN	denotes	activity
T7143	12454-12455	-LRB-	denotes	(
T7145	12455-12457	CD	denotes	29
T7146	12457-12458	,	denotes	,
T7144	12458-12460	CD	denotes	30
T7147	12460-12461	-RRB-	denotes	)
T7148	12461-12462	.	denotes	.
T7149	12462-12584	sentence	denotes	The unpaired segment in Bub82/c-myc/DUE contains one strand of DUE and that in Bub82/c-myc/DUE-C contains another strand.
T7150	12463-12466	DT	denotes	The
T7152	12467-12475	JJ	denotes	unpaired
T7151	12476-12483	NN	denotes	segment
T7154	12484-12486	IN	denotes	in
T7155	12487-12492	NN	denotes	Bub82
T7157	12492-12493	HYPH	denotes	/
T7158	12493-12494	NN	denotes	c
T7160	12494-12495	HYPH	denotes	-
T7159	12495-12498	NN	denotes	myc
T7161	12498-12499	HYPH	denotes	/
T7156	12499-12502	NN	denotes	DUE
T7153	12503-12511	VBZ	denotes	contains
T7162	12512-12515	CD	denotes	one
T7163	12516-12522	NN	denotes	strand
T7164	12523-12525	IN	denotes	of
T7165	12526-12529	NN	denotes	DUE
T7166	12530-12533	CC	denotes	and
T7167	12534-12538	DT	denotes	that
T7169	12539-12541	IN	denotes	in
T7170	12542-12547	NN	denotes	Bub82
T7172	12547-12548	HYPH	denotes	/
T7173	12548-12549	NN	denotes	c
T7175	12549-12550	HYPH	denotes	-
T7174	12550-12553	NN	denotes	myc
T7176	12553-12554	HYPH	denotes	/
T7177	12554-12557	NN	denotes	DUE
T7178	12557-12558	HYPH	denotes	-
T7171	12558-12559	NN	denotes	C
T7168	12560-12568	VBZ	denotes	contains
T7179	12569-12576	DT	denotes	another
T7180	12577-12583	NN	denotes	strand
T7181	12583-12584	.	denotes	.
T7182	12584-12746	sentence	denotes	This DUE is believed to coincide with the initially unwound region of the c-myc origin (29), and its deletion substantially reduced the replicator activity (30).
T7183	12585-12589	DT	denotes	This
T7184	12590-12593	NN	denotes	DUE
T7186	12594-12596	VBZ	denotes	is
T7185	12597-12605	VBN	denotes	believed
T7187	12606-12608	TO	denotes	to
T7188	12609-12617	VB	denotes	coincide
T7189	12618-12622	IN	denotes	with
T7190	12623-12626	DT	denotes	the
T7192	12627-12636	RB	denotes	initially
T7193	12637-12644	JJ	denotes	unwound
T7191	12645-12651	NN	denotes	region
T7194	12652-12654	IN	denotes	of
T7195	12655-12658	DT	denotes	the
T7197	12659-12660	NN	denotes	c
T7199	12660-12661	HYPH	denotes	-
T7198	12661-12664	NN	denotes	myc
T7196	12665-12671	NN	denotes	origin
T7200	12672-12673	-LRB-	denotes	(
T7201	12673-12675	CD	denotes	29
T7202	12675-12676	-RRB-	denotes	)
T7203	12676-12678	,	denotes	,
T7204	12678-12681	CC	denotes	and
T7205	12682-12685	PRP$	denotes	its
T7206	12686-12694	NN	denotes	deletion
T7208	12695-12708	RB	denotes	substantially
T7207	12709-12716	VBD	denotes	reduced
T7209	12717-12720	DT	denotes	the
T7211	12721-12731	NN	denotes	replicator
T7210	12732-12740	NN	denotes	activity
T7212	12741-12742	-LRB-	denotes	(
T7213	12742-12744	CD	denotes	30
T7214	12744-12745	-RRB-	denotes	)
T7215	12745-12746	.	denotes	.
T7216	12746-12992	sentence	denotes	Mcm4/6/7 unwound Bub82/lamin B2 (thymine 48%), Bub82/c-myc/DUE (37%) and Bub82/c-myc/DUE-C (39%) with similar efficiency (Figure 7A), indicating that the sequences from the c-myc origin also can serve as efficient activators of the Mcm helicase.
T7217	12747-12751	NN	denotes	Mcm4
T7219	12751-12752	HYPH	denotes	/
T7220	12752-12753	CD	denotes	6
T7221	12753-12754	HYPH	denotes	/
T7222	12754-12755	CD	denotes	7
T7218	12756-12763	VBN	denotes	unwound
T7223	12764-12769	NN	denotes	Bub82
T7225	12769-12770	HYPH	denotes	/
T7226	12770-12775	NN	denotes	lamin
T7224	12776-12778	NN	denotes	B2
T7227	12779-12780	-LRB-	denotes	(
T7229	12780-12787	NN	denotes	thymine
T7230	12788-12790	CD	denotes	48
T7228	12790-12791	NN	denotes	%
T7231	12791-12792	-RRB-	denotes	)
T7232	12792-12794	,	denotes	,
T7233	12794-12799	NN	denotes	Bub82
T7235	12799-12800	HYPH	denotes	/
T7236	12800-12801	NN	denotes	c
T7238	12801-12802	HYPH	denotes	-
T7237	12802-12805	NN	denotes	myc
T7239	12805-12806	HYPH	denotes	/
T7234	12806-12809	NN	denotes	DUE
T7240	12810-12811	-LRB-	denotes	(
T7242	12811-12813	CD	denotes	37
T7241	12813-12814	NN	denotes	%
T7243	12814-12815	-RRB-	denotes	)
T7244	12816-12819	CC	denotes	and
T7245	12820-12825	NN	denotes	Bub82
T7247	12825-12826	HYPH	denotes	/
T7248	12826-12827	NN	denotes	c
T7250	12827-12828	HYPH	denotes	-
T7249	12828-12831	NN	denotes	myc
T7251	12831-12832	HYPH	denotes	/
T7252	12832-12835	NN	denotes	DUE
T7253	12835-12836	HYPH	denotes	-
T7246	12836-12837	NN	denotes	C
T7254	12838-12839	-LRB-	denotes	(
T7256	12839-12841	CD	denotes	39
T7255	12841-12842	NN	denotes	%
T7257	12842-12843	-RRB-	denotes	)
T7258	12844-12848	IN	denotes	with
T7259	12849-12856	JJ	denotes	similar
T7260	12857-12867	NN	denotes	efficiency
T7261	12868-12869	-LRB-	denotes	(
T7263	12869-12875	NN	denotes	Figure
T7262	12876-12878	NN	denotes	7A
T7264	12878-12879	-RRB-	denotes	)
T7265	12879-12881	,	denotes	,
T7266	12881-12891	VBG	denotes	indicating
T7267	12892-12896	IN	denotes	that
T7269	12897-12900	DT	denotes	the
T7270	12901-12910	NNS	denotes	sequences
T7271	12911-12915	IN	denotes	from
T7272	12916-12919	DT	denotes	the
T7274	12920-12921	NN	denotes	c
T7276	12921-12922	HYPH	denotes	-
T7275	12922-12925	NN	denotes	myc
T7273	12926-12932	NN	denotes	origin
T7277	12933-12937	RB	denotes	also
T7278	12938-12941	MD	denotes	can
T7268	12942-12947	VB	denotes	serve
T7279	12948-12950	IN	denotes	as
T7280	12951-12960	JJ	denotes	efficient
T7281	12961-12971	NNS	denotes	activators
T7282	12972-12974	IN	denotes	of
T7283	12975-12978	DT	denotes	the
T7285	12979-12982	NN	denotes	Mcm
T7284	12983-12991	NN	denotes	helicase
T7286	12991-12992	.	denotes	.
T7287	12992-13258	sentence	denotes	This result also indicates that sequences with 37% thymine content can activate Mcm4/6/7 to displace 24 nt duplex on both sides in a bubble substrate, suggesting that the not only thymine content but also the sequence context may affect the Mcm helicase activation.
T7288	12993-12997	DT	denotes	This
T7289	12998-13004	NN	denotes	result
T7291	13005-13009	RB	denotes	also
T7290	13010-13019	VBZ	denotes	indicates
T7292	13020-13024	IN	denotes	that
T7294	13025-13034	NNS	denotes	sequences
T7295	13035-13039	IN	denotes	with
T7296	13040-13042	CD	denotes	37
T7297	13042-13043	NN	denotes	%
T7299	13044-13051	NN	denotes	thymine
T7298	13052-13059	NN	denotes	content
T7300	13060-13063	MD	denotes	can
T7293	13064-13072	VB	denotes	activate
T7301	13073-13077	NN	denotes	Mcm4
T7302	13077-13078	HYPH	denotes	/
T7303	13078-13079	CD	denotes	6
T7304	13079-13080	HYPH	denotes	/
T7305	13080-13081	CD	denotes	7
T7306	13082-13084	TO	denotes	to
T7307	13085-13093	VB	denotes	displace
T7308	13094-13096	CD	denotes	24
T7309	13097-13099	NN	denotes	nt
T7310	13100-13106	NN	denotes	duplex
T7311	13107-13109	IN	denotes	on
T7312	13110-13114	DT	denotes	both
T7313	13115-13120	NNS	denotes	sides
T7314	13121-13123	IN	denotes	in
T7315	13124-13125	DT	denotes	a
T7317	13126-13132	NN	denotes	bubble
T7316	13133-13142	NN	denotes	substrate
T7318	13142-13144	,	denotes	,
T7319	13144-13154	VBG	denotes	suggesting
T7320	13155-13159	IN	denotes	that
T7322	13160-13163	DT	denotes	the
T7324	13164-13167	RB	denotes	not
T7325	13168-13172	RB	denotes	only
T7326	13173-13180	NN	denotes	thymine
T7323	13181-13188	NN	denotes	content
T7327	13189-13192	CC	denotes	but
T7328	13193-13197	RB	denotes	also
T7329	13198-13201	DT	denotes	the
T7331	13202-13210	NN	denotes	sequence
T7330	13211-13218	NN	denotes	context
T7332	13219-13222	MD	denotes	may
T7321	13223-13229	VB	denotes	affect
T7333	13230-13233	DT	denotes	the
T7335	13234-13237	NN	denotes	Mcm
T7336	13238-13246	NN	denotes	helicase
T7334	13247-13257	NN	denotes	activation
T7337	13257-13258	.	denotes	.
T7338	13258-13446	sentence	denotes	In gel shift assays, MCM4/6/7 bound to all the three bubbles, although the affinity to Bub82/c-myc/DUE and to Bub82/c-myc/DUE-C is slightly lower than that to Bub-82/lamin B2 (Figure 7B).
T7339	13259-13261	IN	denotes	In
T7341	13262-13265	NN	denotes	gel
T7342	13266-13271	NN	denotes	shift
T7343	13272-13278	NNS	denotes	assays
T7344	13278-13280	,	denotes	,
T7345	13280-13284	NN	denotes	MCM4
T7346	13284-13285	HYPH	denotes	/
T7347	13285-13286	CD	denotes	6
T7348	13286-13287	HYPH	denotes	/
T7349	13287-13288	CD	denotes	7
T7340	13289-13294	VBD	denotes	bound
T7350	13295-13297	IN	denotes	to
T7351	13298-13301	PDT	denotes	all
T7353	13302-13305	DT	denotes	the
T7354	13306-13311	CD	denotes	three
T7352	13312-13319	NNS	denotes	bubbles
T7355	13319-13321	,	denotes	,
T7356	13321-13329	IN	denotes	although
T7358	13330-13333	DT	denotes	the
T7359	13334-13342	NN	denotes	affinity
T7360	13343-13345	IN	denotes	to
T7361	13346-13351	NN	denotes	Bub82
T7363	13351-13352	HYPH	denotes	/
T7364	13352-13353	NN	denotes	c
T7366	13353-13354	HYPH	denotes	-
T7365	13354-13357	NN	denotes	myc
T7367	13357-13358	HYPH	denotes	/
T7362	13358-13361	NN	denotes	DUE
T7368	13362-13365	CC	denotes	and
T7369	13366-13368	IN	denotes	to
T7370	13369-13374	NN	denotes	Bub82
T7372	13374-13375	HYPH	denotes	/
T7373	13375-13376	NN	denotes	c
T7375	13376-13377	HYPH	denotes	-
T7374	13377-13380	NN	denotes	myc
T7376	13380-13381	HYPH	denotes	/
T7377	13381-13384	NN	denotes	DUE
T7378	13384-13385	HYPH	denotes	-
T7371	13385-13386	NN	denotes	C
T7357	13387-13389	VBZ	denotes	is
T7379	13390-13398	RB	denotes	slightly
T7380	13399-13404	JJR	denotes	lower
T7381	13405-13409	IN	denotes	than
T7382	13410-13414	DT	denotes	that
T7383	13415-13417	IN	denotes	to
T7384	13418-13421	NN	denotes	Bub
T7386	13421-13422	HYPH	denotes	-
T7387	13422-13424	CD	denotes	82
T7388	13424-13425	HYPH	denotes	/
T7389	13425-13430	NN	denotes	lamin
T7385	13431-13433	NN	denotes	B2
T7390	13434-13435	-LRB-	denotes	(
T7392	13435-13441	NN	denotes	Figure
T7391	13442-13444	NN	denotes	7B
T7393	13444-13445	-RRB-	denotes	)
T7394	13445-13446	.	denotes	.
T7395	13446-13539	sentence	denotes	Therefore, helicase and DNA binding activities do not necessarily correlate with each other.
T7396	13447-13456	RB	denotes	Therefore
T7398	13456-13458	,	denotes	,
T7399	13458-13466	NN	denotes	helicase
T7401	13467-13470	CC	denotes	and
T7402	13471-13474	NN	denotes	DNA
T7403	13475-13482	NN	denotes	binding
T7400	13483-13493	NNS	denotes	activities
T7404	13494-13496	VBP	denotes	do
T7405	13497-13500	RB	denotes	not
T7406	13501-13512	RB	denotes	necessarily
T7397	13513-13522	VB	denotes	correlate
T7407	13523-13527	IN	denotes	with
T7408	13528-13532	DT	denotes	each
T7409	13533-13538	JJ	denotes	other
T7410	13538-13539	.	denotes	.
T7411	13539-13678	sentence	denotes	This was also indicated by our previous results that guanine and cytosine stretches can bind to Mcm but cannot activate the helicase (13).
T7412	13540-13544	DT	denotes	This
T7414	13545-13548	VBD	denotes	was
T7415	13549-13553	RB	denotes	also
T7413	13554-13563	VBN	denotes	indicated
T7416	13564-13566	IN	denotes	by
T7417	13567-13570	PRP$	denotes	our
T7419	13571-13579	JJ	denotes	previous
T7418	13580-13587	NNS	denotes	results
T7420	13588-13592	IN	denotes	that
T7422	13593-13600	NN	denotes	guanine
T7424	13601-13604	CC	denotes	and
T7425	13605-13613	NN	denotes	cytosine
T7423	13614-13623	NNS	denotes	stretches
T7426	13624-13627	MD	denotes	can
T7421	13628-13632	VB	denotes	bind
T7427	13633-13635	IN	denotes	to
T7428	13636-13639	NN	denotes	Mcm
T7429	13640-13643	CC	denotes	but
T7430	13644-13647	MD	denotes	can
T7432	13647-13650	RB	denotes	not
T7431	13651-13659	VB	denotes	activate
T7433	13660-13663	DT	denotes	the
T7434	13664-13672	NN	denotes	helicase
T7435	13673-13674	-LRB-	denotes	(
T7436	13674-13676	CD	denotes	13
T7437	13676-13677	-RRB-	denotes	)
T7438	13677-13678	.	denotes	.
T7722	13680-13683	NN	denotes	DNA
T7723	13684-13693	NN	denotes	unwinding
T7725	13694-13696	IN	denotes	by
T7726	13697-13701	NN	denotes	Mcm4
T7727	13701-13702	HYPH	denotes	/
T7728	13702-13703	CD	denotes	6
T7729	13703-13704	HYPH	denotes	/
T7730	13704-13705	CD	denotes	7
T7731	13706-13708	VBZ	denotes	is
T7724	13709-13718	VBN	denotes	inhibited
T7732	13719-13721	IN	denotes	by
T7733	13722-13725	DT	denotes	the
T7735	13726-13728	NN	denotes	GC
T7737	13728-13729	HYPH	denotes	-
T7736	13729-13733	JJ	denotes	rich
T7734	13734-13743	NNS	denotes	sequences
T7738	13744-13746	IN	denotes	on
T7739	13747-13750	DT	denotes	the
T7741	13751-13757	NN	denotes	duplex
T7740	13758-13765	NN	denotes	segment
T7742	13765-13892	sentence	denotes	Our results indicate that thymine-rich single-stranded DNA is required for initial loading and activation of the Mcm helicase.
T7743	13766-13769	PRP$	denotes	Our
T7744	13770-13777	NNS	denotes	results
T7745	13778-13786	VBP	denotes	indicate
T7746	13787-13791	IN	denotes	that
T7748	13792-13799	NN	denotes	thymine
T7750	13799-13800	HYPH	denotes	-
T7749	13800-13804	JJ	denotes	rich
T7752	13805-13811	JJ	denotes	single
T7754	13811-13812	HYPH	denotes	-
T7753	13812-13820	VBN	denotes	stranded
T7751	13821-13824	NN	denotes	DNA
T7755	13825-13827	VBZ	denotes	is
T7747	13828-13836	VBN	denotes	required
T7756	13837-13840	IN	denotes	for
T7757	13841-13848	JJ	denotes	initial
T7758	13849-13856	NN	denotes	loading
T7759	13857-13860	CC	denotes	and
T7760	13861-13871	NN	denotes	activation
T7761	13872-13874	IN	denotes	of
T7762	13875-13878	DT	denotes	the
T7764	13879-13882	NN	denotes	Mcm
T7763	13883-13891	NN	denotes	helicase
T7765	13891-13892	.	denotes	.
T7766	13892-13996	sentence	denotes	However, it is not known whether thymine sequences are required for processive unwinding of duplex DNA.
T7767	13893-13900	RB	denotes	However
T7769	13900-13902	,	denotes	,
T7770	13902-13904	PRP	denotes	it
T7771	13905-13907	VBZ	denotes	is
T7772	13908-13911	RB	denotes	not
T7768	13912-13917	VBN	denotes	known
T7773	13918-13925	IN	denotes	whether
T7775	13926-13933	NN	denotes	thymine
T7776	13934-13943	NNS	denotes	sequences
T7777	13944-13947	VBP	denotes	are
T7774	13948-13956	VBN	denotes	required
T7778	13957-13960	IN	denotes	for
T7779	13961-13971	JJ	denotes	processive
T7780	13972-13981	NN	denotes	unwinding
T7781	13982-13984	IN	denotes	of
T7782	13985-13991	NN	denotes	duplex
T7783	13992-13995	NN	denotes	DNA
T7784	13995-13996	.	denotes	.
T7785	13996-14112	sentence	denotes	Therefore, we have examined whether the nucleotide composition of the duplex region affects its unwinding activity.
T7786	13997-14006	RB	denotes	Therefore
T7788	14006-14008	,	denotes	,
T7789	14008-14010	PRP	denotes	we
T7790	14011-14015	VBP	denotes	have
T7787	14016-14024	VBN	denotes	examined
T7791	14025-14032	IN	denotes	whether
T7793	14033-14036	DT	denotes	the
T7795	14037-14047	NN	denotes	nucleotide
T7794	14048-14059	NN	denotes	composition
T7796	14060-14062	IN	denotes	of
T7797	14063-14066	DT	denotes	the
T7799	14067-14073	NN	denotes	duplex
T7798	14074-14080	NN	denotes	region
T7792	14081-14088	VBZ	denotes	affects
T7800	14089-14092	PRP$	denotes	its
T7802	14093-14102	NN	denotes	unwinding
T7801	14103-14111	NN	denotes	activity
T7803	14111-14112	.	denotes	.
T7804	14112-14250	sentence	denotes	To address this issue, we constructed a series of T-tailed Y-fork structures (T-fork) containing various sequences in the duplex segment.
T7805	14113-14115	TO	denotes	To
T7806	14116-14123	VB	denotes	address
T7808	14124-14128	DT	denotes	this
T7809	14129-14134	NN	denotes	issue
T7810	14134-14136	,	denotes	,
T7811	14136-14138	PRP	denotes	we
T7807	14139-14150	VBD	denotes	constructed
T7812	14151-14152	DT	denotes	a
T7813	14153-14159	NN	denotes	series
T7814	14160-14162	IN	denotes	of
T7815	14163-14164	NN	denotes	T
T7817	14164-14165	HYPH	denotes	-
T7816	14165-14171	VBN	denotes	tailed
T7819	14172-14173	NN	denotes	Y
T7821	14173-14174	HYPH	denotes	-
T7820	14174-14178	NN	denotes	fork
T7818	14179-14189	NNS	denotes	structures
T7822	14190-14191	-LRB-	denotes	(
T7823	14191-14192	NN	denotes	T
T7825	14192-14193	HYPH	denotes	-
T7824	14193-14197	NN	denotes	fork
T7826	14197-14198	-RRB-	denotes	)
T7827	14199-14209	VBG	denotes	containing
T7828	14210-14217	JJ	denotes	various
T7829	14218-14227	NNS	denotes	sequences
T7830	14228-14230	IN	denotes	in
T7831	14231-14234	DT	denotes	the
T7833	14235-14241	NN	denotes	duplex
T7832	14242-14249	NN	denotes	segment
T7834	14249-14250	.	denotes	.
T7835	14250-14323	sentence	denotes	They carried varied contents of cytosine residues on the 3′-tail strand.
T7836	14251-14255	PRP	denotes	They
T7837	14256-14263	VBD	denotes	carried
T7838	14264-14270	VBN	denotes	varied
T7839	14271-14279	NNS	denotes	contents
T7840	14280-14282	IN	denotes	of
T7841	14283-14291	NN	denotes	cytosine
T7842	14292-14300	NNS	denotes	residues
T7843	14301-14303	IN	denotes	on
T7844	14304-14307	DT	denotes	the
T7846	14308-14309	CD	denotes	3
T7848	14309-14310	SYM	denotes	′
T7849	14310-14311	HYPH	denotes	-
T7847	14311-14315	NN	denotes	tail
T7845	14316-14322	NN	denotes	strand
T7850	14322-14323	.	denotes	.
T7851	14323-14500	sentence	denotes	In gel shift assays, Mcm4/6/7 bound to these Y-fork substrates with identical affinity (Figure 8A), consistent with the notion that the Mcm4/6/7 binds to single-stranded tails.
T7852	14324-14326	IN	denotes	In
T7854	14327-14330	NN	denotes	gel
T7855	14331-14336	NN	denotes	shift
T7856	14337-14343	NNS	denotes	assays
T7857	14343-14345	,	denotes	,
T7858	14345-14349	NN	denotes	Mcm4
T7859	14349-14350	HYPH	denotes	/
T7860	14350-14351	CD	denotes	6
T7861	14351-14352	HYPH	denotes	/
T7862	14352-14353	CD	denotes	7
T7853	14354-14359	VBD	denotes	bound
T7863	14360-14362	IN	denotes	to
T7864	14363-14368	DT	denotes	these
T7866	14369-14370	NN	denotes	Y
T7868	14370-14371	HYPH	denotes	-
T7867	14371-14375	NN	denotes	fork
T7865	14376-14386	NNS	denotes	substrates
T7869	14387-14391	IN	denotes	with
T7870	14392-14401	JJ	denotes	identical
T7871	14402-14410	NN	denotes	affinity
T7872	14411-14412	-LRB-	denotes	(
T7874	14412-14418	NN	denotes	Figure
T7873	14419-14421	NN	denotes	8A
T7875	14421-14422	-RRB-	denotes	)
T7876	14422-14424	,	denotes	,
T7877	14424-14434	JJ	denotes	consistent
T7878	14435-14439	IN	denotes	with
T7879	14440-14443	DT	denotes	the
T7880	14444-14450	NN	denotes	notion
T7881	14451-14455	IN	denotes	that
T7883	14456-14459	DT	denotes	the
T7884	14460-14464	NN	denotes	Mcm4
T7885	14464-14465	HYPH	denotes	/
T7886	14465-14466	CD	denotes	6
T7887	14466-14467	HYPH	denotes	/
T7888	14467-14468	CD	denotes	7
T7882	14469-14474	VBZ	denotes	binds
T7889	14475-14477	IN	denotes	to
T7890	14478-14484	JJ	denotes	single
T7892	14484-14485	HYPH	denotes	-
T7891	14485-14493	VBN	denotes	stranded
T7893	14494-14499	NNS	denotes	tails
T7894	14499-14500	.	denotes	.
T7895	14500-14652	sentence	denotes	However, in DNA helicase assays, T-fork/(C:G)49 was hardly displaced by Mcm4/6/7, but was readily displaced by SV40 T-antigen DNA helicase (Figure 8B).
T7896	14501-14508	RB	denotes	However
T7898	14508-14510	,	denotes	,
T7899	14510-14512	IN	denotes	in
T7900	14513-14516	NN	denotes	DNA
T7901	14517-14525	NN	denotes	helicase
T7902	14526-14532	NNS	denotes	assays
T7903	14532-14534	,	denotes	,
T7904	14534-14535	NN	denotes	T
T7906	14535-14536	HYPH	denotes	-
T7905	14536-14540	NN	denotes	fork
T7907	14540-14541	HYPH	denotes	/
T7908	14541-14542	-LRB-	denotes	(
T7910	14542-14543	NN	denotes	C
T7911	14543-14544	:	denotes	:
T7909	14544-14545	NN	denotes	G
T7912	14545-14546	-RRB-	denotes	)
T7913	14546-14548	CD	denotes	49
T7914	14549-14552	VBD	denotes	was
T7915	14553-14559	RB	denotes	hardly
T7897	14560-14569	VBN	denotes	displaced
T7916	14570-14572	IN	denotes	by
T7917	14573-14577	NN	denotes	Mcm4
T7918	14577-14578	HYPH	denotes	/
T7919	14578-14579	CD	denotes	6
T7920	14579-14580	HYPH	denotes	/
T7921	14580-14581	CD	denotes	7
T7922	14581-14583	,	denotes	,
T7923	14583-14586	CC	denotes	but
T7924	14587-14590	VBD	denotes	was
T7926	14591-14598	RB	denotes	readily
T7925	14599-14608	VBN	denotes	displaced
T7927	14609-14611	IN	denotes	by
T7928	14612-14616	NN	denotes	SV40
T7930	14617-14618	NN	denotes	T
T7932	14618-14619	HYPH	denotes	-
T7931	14619-14626	NN	denotes	antigen
T7933	14627-14630	NN	denotes	DNA
T7929	14631-14639	NN	denotes	helicase
T7934	14640-14641	-LRB-	denotes	(
T7936	14641-14647	NN	denotes	Figure
T7935	14648-14650	NN	denotes	8B
T7937	14650-14651	-RRB-	denotes	)
T7938	14651-14652	.	denotes	.
T7939	14652-14818	sentence	denotes	When thymine or adenine is inserted as every third nucleotide (repeats of CCT or CCA), the extent of unwinding increased (T-fork/(CCT:GGA)16 and T-fork/(CCA:GGT)16).
T7940	14653-14657	WRB	denotes	When
T7942	14658-14665	NN	denotes	thymine
T7943	14666-14668	CC	denotes	or
T7944	14669-14676	NN	denotes	adenine
T7945	14677-14679	VBZ	denotes	is
T7941	14680-14688	VBN	denotes	inserted
T7947	14689-14691	IN	denotes	as
T7948	14692-14697	DT	denotes	every
T7950	14698-14703	JJ	denotes	third
T7949	14704-14714	NN	denotes	nucleotide
T7951	14715-14716	-LRB-	denotes	(
T7952	14716-14723	NNS	denotes	repeats
T7953	14724-14726	IN	denotes	of
T7954	14727-14730	NN	denotes	CCT
T7955	14731-14733	CC	denotes	or
T7956	14734-14737	NN	denotes	CCA
T7957	14737-14738	-RRB-	denotes	)
T7958	14738-14740	,	denotes	,
T7959	14740-14743	DT	denotes	the
T7960	14744-14750	NN	denotes	extent
T7961	14751-14753	IN	denotes	of
T7962	14754-14763	NN	denotes	unwinding
T7946	14764-14773	VBD	denotes	increased
T7963	14774-14775	-LRB-	denotes	(
T7965	14775-14776	NN	denotes	T
T7967	14776-14777	HYPH	denotes	-
T7966	14777-14781	NN	denotes	fork
T7968	14781-14782	HYPH	denotes	/
T7969	14782-14783	-LRB-	denotes	(
T7970	14783-14786	NN	denotes	CCT
T7971	14786-14787	:	denotes	:
T7964	14787-14790	NN	denotes	GGA
T7972	14790-14791	-RRB-	denotes	)
T7973	14791-14793	CD	denotes	16
T7974	14794-14797	CC	denotes	and
T7975	14798-14799	NN	denotes	T
T7977	14799-14800	HYPH	denotes	-
T7976	14800-14804	NN	denotes	fork
T7979	14804-14805	HYPH	denotes	/
T7980	14805-14806	-LRB-	denotes	(
T7981	14806-14809	NN	denotes	CCA
T7982	14809-14810	:	denotes	:
T7978	14810-14813	NN	denotes	GGT
T7983	14813-14814	-RRB-	denotes	)
T7984	14814-14816	CD	denotes	16
T7985	14816-14817	-RRB-	denotes	)
T7986	14817-14818	.	denotes	.
T7987	14818-15023	sentence	denotes	The efficiency of unwinding is roughly correlated with the content of GC pairs in the duplex segment [T-fork/(CCAA:GGTT)12, T-fork/(CCTT:GGAA)12, T-fork/(CAAA:GTTT)12 and T-fork/(CTTT:GAAA)12; Figure 8B].
T7988	14819-14822	DT	denotes	The
T7989	14823-14833	NN	denotes	efficiency
T7991	14834-14836	IN	denotes	of
T7992	14837-14846	NN	denotes	unwinding
T7993	14847-14849	VBZ	denotes	is
T7994	14850-14857	RB	denotes	roughly
T7990	14858-14868	VBN	denotes	correlated
T7995	14869-14873	IN	denotes	with
T7996	14874-14877	DT	denotes	the
T7997	14878-14885	NN	denotes	content
T7998	14886-14888	IN	denotes	of
T7999	14889-14891	NN	denotes	GC
T8000	14892-14897	NNS	denotes	pairs
T8001	14898-14900	IN	denotes	in
T8002	14901-14904	DT	denotes	the
T8004	14905-14911	NN	denotes	duplex
T8003	14912-14919	NN	denotes	segment
T8005	14920-14921	-LRB-	denotes	[
T8007	14921-14922	NN	denotes	T
T8009	14922-14923	HYPH	denotes	-
T8008	14923-14927	NN	denotes	fork
T8010	14927-14928	HYPH	denotes	/
T8011	14928-14929	-LRB-	denotes	(
T8012	14929-14933	NN	denotes	CCAA
T8013	14933-14934	:	denotes	:
T8006	14934-14938	NN	denotes	GGTT
T8014	14938-14939	-RRB-	denotes	)
T8015	14939-14941	CD	denotes	12
T8016	14941-14943	,	denotes	,
T8017	14943-14944	NN	denotes	T
T8019	14944-14945	HYPH	denotes	-
T8018	14945-14949	NN	denotes	fork
T8021	14949-14950	HYPH	denotes	/
T8022	14950-14951	-LRB-	denotes	(
T8023	14951-14955	NN	denotes	CCTT
T8024	14955-14956	:	denotes	:
T8020	14956-14960	NN	denotes	GGAA
T8025	14960-14961	-RRB-	denotes	)
T8026	14961-14963	CD	denotes	12
T8027	14963-14965	,	denotes	,
T8028	14965-14966	NN	denotes	T
T8030	14966-14967	HYPH	denotes	-
T8029	14967-14971	NN	denotes	fork
T8032	14971-14972	HYPH	denotes	/
T8033	14972-14973	-LRB-	denotes	(
T8034	14973-14977	NN	denotes	CAAA
T8035	14977-14978	:	denotes	:
T8031	14978-14982	NN	denotes	GTTT
T8036	14982-14983	-RRB-	denotes	)
T8037	14983-14985	CD	denotes	12
T8038	14986-14989	CC	denotes	and
T8039	14990-14991	NN	denotes	T
T8041	14991-14992	HYPH	denotes	-
T8040	14992-14996	NN	denotes	fork
T8043	14996-14997	HYPH	denotes	/
T8044	14997-14998	-LRB-	denotes	(
T8045	14998-15002	NN	denotes	CTTT
T8046	15002-15003	:	denotes	:
T8042	15003-15007	NN	denotes	GAAA
T8047	15007-15008	-RRB-	denotes	)
T8048	15008-15010	CD	denotes	12
T8049	15010-15011	:	denotes	;
T8051	15012-15018	NN	denotes	Figure
T8050	15019-15021	NN	denotes	8B
T8052	15021-15022	-RRB-	denotes	]
T8053	15022-15023	.	denotes	.
T8054	15023-15119	sentence	denotes	It appears that the duplex segment containing <50% GC pairs is efficiently unwound (Figure 8C).
T8055	15024-15026	PRP	denotes	It
T8056	15027-15034	VBZ	denotes	appears
T8057	15035-15039	IN	denotes	that
T8059	15040-15043	DT	denotes	the
T8061	15044-15050	NN	denotes	duplex
T8060	15051-15058	NN	denotes	segment
T8062	15059-15069	VBG	denotes	containing
T8063	15070-15071	SYM	denotes	<
T8064	15071-15073	CD	denotes	50
T8065	15073-15074	NN	denotes	%
T8067	15075-15077	NN	denotes	GC
T8066	15078-15083	NNS	denotes	pairs
T8068	15084-15086	VBZ	denotes	is
T8069	15087-15098	RB	denotes	efficiently
T8058	15099-15106	VBN	denotes	unwound
T8070	15107-15108	-LRB-	denotes	(
T8072	15108-15114	NN	denotes	Figure
T8071	15115-15117	NN	denotes	8C
T8073	15117-15118	-RRB-	denotes	)
T8074	15118-15119	.	denotes	.
T8075	15119-15232	sentence	denotes	We next examined the effect of GC-rich segments on a partial heteroduplex DNA on a single-stranded circular DNA.
T8076	15120-15122	PRP	denotes	We
T8078	15123-15127	RB	denotes	next
T8077	15128-15136	VBD	denotes	examined
T8079	15137-15140	DT	denotes	the
T8080	15141-15147	NN	denotes	effect
T8081	15148-15150	IN	denotes	of
T8082	15151-15153	NN	denotes	GC
T8084	15153-15154	HYPH	denotes	-
T8083	15154-15158	JJ	denotes	rich
T8085	15159-15167	NNS	denotes	segments
T8086	15168-15170	IN	denotes	on
T8087	15171-15172	DT	denotes	a
T8089	15173-15180	JJ	denotes	partial
T8090	15181-15193	NN	denotes	heteroduplex
T8088	15194-15197	NN	denotes	DNA
T8091	15198-15200	IN	denotes	on
T8092	15201-15202	DT	denotes	a
T8094	15203-15209	JJ	denotes	single
T8096	15209-15210	HYPH	denotes	-
T8095	15210-15218	VBN	denotes	stranded
T8097	15219-15227	JJ	denotes	circular
T8093	15228-15231	NN	denotes	DNA
T8098	15231-15232	.	denotes	.
T8099	15232-15490	sentence	denotes	We have constructed two sets of 5′-tailed partial heteroduplex DNA substrates containing duplex regions of variable lengths; one on M13mp18 vector and the other on M13mp19 containing a 66 nt long G-rich segment downstream of the hybridizing oligonucleotide.
T8100	15233-15235	PRP	denotes	We
T8102	15236-15240	VBP	denotes	have
T8101	15241-15252	VBN	denotes	constructed
T8103	15253-15256	CD	denotes	two
T8104	15257-15261	NNS	denotes	sets
T8105	15262-15264	IN	denotes	of
T8106	15265-15266	CD	denotes	5
T8108	15266-15267	SYM	denotes	′
T8109	15267-15268	HYPH	denotes	-
T8107	15268-15274	VBN	denotes	tailed
T8111	15275-15282	JJ	denotes	partial
T8112	15283-15295	NN	denotes	heteroduplex
T8113	15296-15299	NN	denotes	DNA
T8110	15300-15310	NNS	denotes	substrates
T8114	15311-15321	VBG	denotes	containing
T8115	15322-15328	NN	denotes	duplex
T8116	15329-15336	NNS	denotes	regions
T8117	15337-15339	IN	denotes	of
T8118	15340-15348	JJ	denotes	variable
T8119	15349-15356	NNS	denotes	lengths
T8120	15356-15357	:	denotes	;
T8121	15358-15361	CD	denotes	one
T8122	15362-15364	IN	denotes	on
T8123	15365-15372	NN	denotes	M13mp18
T8124	15373-15379	NN	denotes	vector
T8125	15380-15383	CC	denotes	and
T8126	15384-15387	DT	denotes	the
T8127	15388-15393	JJ	denotes	other
T8128	15394-15396	IN	denotes	on
T8129	15397-15404	NN	denotes	M13mp19
T8130	15405-15415	VBG	denotes	containing
T8131	15416-15417	DT	denotes	a
T8133	15418-15420	CD	denotes	66
T8134	15421-15423	NN	denotes	nt
T8135	15424-15428	JJ	denotes	long
T8136	15429-15430	NN	denotes	G
T8138	15430-15431	HYPH	denotes	-
T8137	15431-15435	JJ	denotes	rich
T8132	15436-15443	NN	denotes	segment
T8139	15444-15454	RB	denotes	downstream
T8140	15455-15457	IN	denotes	of
T8141	15458-15461	DT	denotes	the
T8143	15462-15473	VBG	denotes	hybridizing
T8142	15474-15489	NN	denotes	oligonucleotide
T8144	15489-15490	.	denotes	.
T8145	15490-15661	sentence	denotes	We found that the Mcm4/6/7 helicase can displace duplex DNA up to 350 nt long on its own on the former substrate (Figure 8D, left), consistent with previous results (13).
T8146	15491-15493	PRP	denotes	We
T8147	15494-15499	VBD	denotes	found
T8148	15500-15504	IN	denotes	that
T8150	15505-15508	DT	denotes	the
T8152	15509-15513	NN	denotes	Mcm4
T8153	15513-15514	HYPH	denotes	/
T8154	15514-15515	CD	denotes	6
T8155	15515-15516	HYPH	denotes	/
T8156	15516-15517	CD	denotes	7
T8151	15518-15526	NN	denotes	helicase
T8157	15527-15530	MD	denotes	can
T8149	15531-15539	VB	denotes	displace
T8158	15540-15546	NN	denotes	duplex
T8159	15547-15550	NN	denotes	DNA
T8160	15551-15553	IN	denotes	up
T8162	15554-15556	IN	denotes	to
T8161	15557-15560	CD	denotes	350
T8163	15561-15563	NN	denotes	nt
T8164	15564-15568	JJ	denotes	long
T8165	15569-15571	IN	denotes	on
T8166	15572-15575	PRP$	denotes	its
T8167	15576-15579	JJ	denotes	own
T8168	15580-15582	IN	denotes	on
T8169	15583-15586	DT	denotes	the
T8171	15587-15593	JJ	denotes	former
T8170	15594-15603	NN	denotes	substrate
T8172	15604-15605	-LRB-	denotes	(
T8174	15605-15611	NN	denotes	Figure
T8173	15612-15614	NN	denotes	8D
T8175	15614-15616	,	denotes	,
T8176	15616-15620	JJ	denotes	left
T8177	15620-15621	-RRB-	denotes	)
T8178	15621-15623	,	denotes	,
T8179	15623-15633	JJ	denotes	consistent
T8180	15634-15638	IN	denotes	with
T8181	15639-15647	JJ	denotes	previous
T8182	15648-15655	NNS	denotes	results
T8183	15656-15657	-LRB-	denotes	(
T8184	15657-15659	CD	denotes	13
T8185	15659-15660	-RRB-	denotes	)
T8186	15660-15661	.	denotes	.
T8187	15661-15908	sentence	denotes	In contrast, displacement was inhibited over the GC-rich segments on the latter substrate (Figure 8D, right panel), although displacement up to 250 nt in length, albeit at a reduced level, was observed at a high concentration of Mcm4/6/7 complex.
T8188	15662-15664	IN	denotes	In
T8190	15665-15673	NN	denotes	contrast
T8191	15673-15675	,	denotes	,
T8192	15675-15687	NN	denotes	displacement
T8193	15688-15691	VBD	denotes	was
T8189	15692-15701	VBN	denotes	inhibited
T8194	15702-15706	IN	denotes	over
T8195	15707-15710	DT	denotes	the
T8197	15711-15713	NN	denotes	GC
T8199	15713-15714	HYPH	denotes	-
T8198	15714-15718	JJ	denotes	rich
T8196	15719-15727	NNS	denotes	segments
T8200	15728-15730	IN	denotes	on
T8201	15731-15734	DT	denotes	the
T8203	15735-15741	JJ	denotes	latter
T8202	15742-15751	NN	denotes	substrate
T8204	15752-15753	-LRB-	denotes	(
T8206	15753-15759	NN	denotes	Figure
T8207	15760-15762	NN	denotes	8D
T8208	15762-15764	,	denotes	,
T8209	15764-15769	JJ	denotes	right
T8205	15770-15775	NN	denotes	panel
T8210	15775-15776	-RRB-	denotes	)
T8211	15776-15778	,	denotes	,
T8212	15778-15786	IN	denotes	although
T8214	15787-15799	NN	denotes	displacement
T8215	15800-15802	IN	denotes	up
T8217	15803-15805	IN	denotes	to
T8216	15806-15809	CD	denotes	250
T8218	15810-15812	NN	denotes	nt
T8219	15813-15815	IN	denotes	in
T8220	15816-15822	NN	denotes	length
T8221	15822-15824	,	denotes	,
T8222	15824-15830	IN	denotes	albeit
T8223	15831-15833	IN	denotes	at
T8224	15834-15835	DT	denotes	a
T8226	15836-15843	VBN	denotes	reduced
T8225	15844-15849	NN	denotes	level
T8227	15849-15851	,	denotes	,
T8228	15851-15854	VBD	denotes	was
T8213	15855-15863	VBN	denotes	observed
T8229	15864-15866	IN	denotes	at
T8230	15867-15868	DT	denotes	a
T8232	15869-15873	JJ	denotes	high
T8231	15874-15887	NN	denotes	concentration
T8233	15888-15890	IN	denotes	of
T8234	15891-15895	NN	denotes	Mcm4
T8236	15895-15896	HYPH	denotes	/
T8237	15896-15897	CD	denotes	6
T8238	15897-15898	HYPH	denotes	/
T8239	15898-15899	CD	denotes	7
T8235	15900-15907	NN	denotes	complex
T8240	15907-15908	.	denotes	.
T8241	15908-16045	sentence	denotes	This result is consistent with that on Y-fork and indicates that GC-rich duplex segment is inhibitory for unwinding by the Mcm helicase.
T8242	15909-15913	DT	denotes	This
T8243	15914-15920	NN	denotes	result
T8244	15921-15923	VBZ	denotes	is
T8245	15924-15934	JJ	denotes	consistent
T8246	15935-15939	IN	denotes	with
T8247	15940-15944	DT	denotes	that
T8248	15945-15947	IN	denotes	on
T8249	15948-15949	NN	denotes	Y
T8251	15949-15950	HYPH	denotes	-
T8250	15950-15954	NN	denotes	fork
T8252	15955-15958	CC	denotes	and
T8253	15959-15968	VBZ	denotes	indicates
T8254	15969-15973	IN	denotes	that
T8256	15974-15976	NN	denotes	GC
T8258	15976-15977	HYPH	denotes	-
T8257	15977-15981	JJ	denotes	rich
T8260	15982-15988	NN	denotes	duplex
T8259	15989-15996	NN	denotes	segment
T8255	15997-15999	VBZ	denotes	is
T8261	16000-16010	JJ	denotes	inhibitory
T8262	16011-16014	IN	denotes	for
T8263	16015-16024	NN	denotes	unwinding
T8264	16025-16027	IN	denotes	by
T8265	16028-16031	DT	denotes	the
T8267	16032-16035	NN	denotes	Mcm
T8266	16036-16044	NN	denotes	helicase
T8268	16044-16045	.	denotes	.
T8269	16045-16210	sentence	denotes	These results may indicate that the presence of thymine–adenine pairs with a certain frequency in the duplex region may facilitate continuous unwinding by Mcm4/6/7.
T8270	16046-16051	DT	denotes	These
T8271	16052-16059	NNS	denotes	results
T8273	16060-16063	MD	denotes	may
T8272	16064-16072	VB	denotes	indicate
T8274	16073-16077	IN	denotes	that
T8276	16078-16081	DT	denotes	the
T8277	16082-16090	NN	denotes	presence
T8278	16091-16093	IN	denotes	of
T8279	16094-16101	NN	denotes	thymine
T8281	16101-16102	HYPH	denotes	–
T8280	16102-16109	NN	denotes	adenine
T8282	16110-16115	NNS	denotes	pairs
T8283	16116-16120	IN	denotes	with
T8284	16121-16122	DT	denotes	a
T8286	16123-16130	JJ	denotes	certain
T8285	16131-16140	NN	denotes	frequency
T8287	16141-16143	IN	denotes	in
T8288	16144-16147	DT	denotes	the
T8290	16148-16154	NN	denotes	duplex
T8289	16155-16161	NN	denotes	region
T8291	16162-16165	MD	denotes	may
T8275	16166-16176	VB	denotes	facilitate
T8292	16177-16187	JJ	denotes	continuous
T8293	16188-16197	NN	denotes	unwinding
T8294	16198-16200	IN	denotes	by
T8295	16201-16205	NN	denotes	Mcm4
T8296	16205-16206	HYPH	denotes	/
T8297	16206-16207	CD	denotes	6
T8298	16207-16208	HYPH	denotes	/
T8299	16208-16209	CD	denotes	7
T8300	16209-16210	.	denotes	.
T8301	16210-16319	sentence	denotes	Alternatively, Mcm4/6/7 is not efficient enough to displace thermodynamically stable GC-base-paired segment.
T8302	16211-16224	RB	denotes	Alternatively
T8304	16224-16226	,	denotes	,
T8305	16226-16230	NN	denotes	Mcm4
T8306	16230-16231	HYPH	denotes	/
T8307	16231-16232	CD	denotes	6
T8308	16232-16233	HYPH	denotes	/
T8309	16233-16234	CD	denotes	7
T8303	16235-16237	VBZ	denotes	is
T8310	16238-16241	RB	denotes	not
T8311	16242-16251	JJ	denotes	efficient
T8312	16252-16258	RB	denotes	enough
T8313	16259-16261	TO	denotes	to
T8314	16262-16270	VB	denotes	displace
T8315	16271-16288	RB	denotes	thermodynamically
T8316	16289-16295	JJ	denotes	stable
T8318	16296-16298	NN	denotes	GC
T8320	16298-16299	HYPH	denotes	-
T8321	16299-16303	NN	denotes	base
T8322	16303-16304	HYPH	denotes	-
T8319	16304-16310	VBN	denotes	paired
T8317	16311-16318	NN	denotes	segment
T8323	16318-16319	.	denotes	.
T8324	16319-16464	sentence	denotes	In order to distinguish these two possibilities, we designed a new T-fork substrate containing inosine (I) residue instead of guanosine residue.
T8325	16320-16322	IN	denotes	In
T8327	16323-16328	NN	denotes	order
T8328	16329-16331	TO	denotes	to
T8329	16332-16343	VB	denotes	distinguish
T8330	16344-16349	DT	denotes	these
T8332	16350-16353	CD	denotes	two
T8331	16354-16367	NNS	denotes	possibilities
T8333	16367-16369	,	denotes	,
T8334	16369-16371	PRP	denotes	we
T8326	16372-16380	VBD	denotes	designed
T8335	16381-16382	DT	denotes	a
T8337	16383-16386	JJ	denotes	new
T8338	16387-16388	NN	denotes	T
T8340	16388-16389	HYPH	denotes	-
T8339	16389-16393	NN	denotes	fork
T8336	16394-16403	NN	denotes	substrate
T8341	16404-16414	VBG	denotes	containing
T8342	16415-16422	NN	denotes	inosine
T8344	16423-16424	-LRB-	denotes	(
T8345	16424-16425	NN	denotes	I
T8346	16425-16426	-RRB-	denotes	)
T8343	16427-16434	NN	denotes	residue
T8347	16435-16442	RB	denotes	instead
T8348	16443-16445	IN	denotes	of
T8349	16446-16455	NN	denotes	guanosine
T8350	16456-16463	NN	denotes	residue
T8351	16463-16464	.	denotes	.
T8352	16464-16637	sentence	denotes	The thermal stability of the I:C base pair is lower than that of the G:C base pair in duplex DNA due to loss of one hydrogen bond and is even lower than A:T base pair (31).
T8353	16465-16468	DT	denotes	The
T8355	16469-16476	JJ	denotes	thermal
T8354	16477-16486	NN	denotes	stability
T8357	16487-16489	IN	denotes	of
T8358	16490-16493	DT	denotes	the
T8360	16494-16495	NN	denotes	I
T8362	16495-16496	:	denotes	:
T8361	16496-16497	NN	denotes	C
T8363	16498-16502	NN	denotes	base
T8359	16503-16507	NN	denotes	pair
T8356	16508-16510	VBZ	denotes	is
T8364	16511-16516	JJR	denotes	lower
T8365	16517-16521	IN	denotes	than
T8366	16522-16526	DT	denotes	that
T8367	16527-16529	IN	denotes	of
T8368	16530-16533	DT	denotes	the
T8370	16534-16535	NN	denotes	G
T8372	16535-16536	:	denotes	:
T8371	16536-16537	NN	denotes	C
T8373	16538-16542	NN	denotes	base
T8369	16543-16547	NN	denotes	pair
T8374	16548-16550	IN	denotes	in
T8375	16551-16557	NN	denotes	duplex
T8376	16558-16561	NN	denotes	DNA
T8377	16562-16565	IN	denotes	due
T8378	16566-16568	IN	denotes	to
T8379	16569-16573	NN	denotes	loss
T8380	16574-16576	IN	denotes	of
T8381	16577-16580	CD	denotes	one
T8383	16581-16589	NN	denotes	hydrogen
T8382	16590-16594	NN	denotes	bond
T8384	16595-16598	CC	denotes	and
T8385	16599-16601	VBZ	denotes	is
T8386	16602-16606	RB	denotes	even
T8387	16607-16612	JJR	denotes	lower
T8388	16613-16617	IN	denotes	than
T8389	16618-16619	NN	denotes	A
T8391	16619-16620	:	denotes	:
T8390	16620-16621	NN	denotes	T
T8393	16622-16626	NN	denotes	base
T8392	16627-16631	NN	denotes	pair
T8394	16632-16633	-LRB-	denotes	(
T8395	16633-16635	CD	denotes	31
T8396	16635-16636	-RRB-	denotes	)
T8397	16636-16637	.	denotes	.
T8398	16637-16768	sentence	denotes	We constructed the T-tailed fork substrates containing the (GCC:CGG)10, (GCC:CIG)10 or (GAA:CTT)10 duplex DNA segment (Figure 8E).
T8399	16638-16640	PRP	denotes	We
T8400	16641-16652	VBD	denotes	constructed
T8401	16653-16656	DT	denotes	the
T8403	16657-16658	NN	denotes	T
T8405	16658-16659	HYPH	denotes	-
T8404	16659-16665	VBN	denotes	tailed
T8406	16666-16670	NN	denotes	fork
T8402	16671-16681	NNS	denotes	substrates
T8407	16682-16692	VBG	denotes	containing
T8408	16693-16696	DT	denotes	the
T8410	16697-16698	-LRB-	denotes	(
T8412	16698-16701	NN	denotes	GCC
T8413	16701-16702	:	denotes	:
T8411	16702-16705	NN	denotes	CGG
T8414	16705-16706	-RRB-	denotes	)
T8415	16706-16708	CD	denotes	10
T8416	16708-16710	,	denotes	,
T8417	16710-16711	-LRB-	denotes	(
T8419	16711-16714	NN	denotes	GCC
T8420	16714-16715	:	denotes	:
T8418	16715-16718	NN	denotes	CIG
T8421	16718-16719	-RRB-	denotes	)
T8422	16719-16721	CD	denotes	10
T8423	16722-16724	CC	denotes	or
T8424	16725-16726	-LRB-	denotes	(
T8426	16726-16729	NN	denotes	GAA
T8427	16729-16730	:	denotes	:
T8425	16730-16733	NN	denotes	CTT
T8428	16733-16734	-RRB-	denotes	)
T8429	16734-16736	CD	denotes	10
T8430	16737-16743	NN	denotes	duplex
T8431	16744-16747	NN	denotes	DNA
T8409	16748-16755	NN	denotes	segment
T8432	16756-16757	-LRB-	denotes	(
T8434	16757-16763	NN	denotes	Figure
T8433	16764-16766	NN	denotes	8E
T8435	16766-16767	-RRB-	denotes	)
T8436	16767-16768	.	denotes	.
T8437	16768-16914	sentence	denotes	Mcm4/6/7 hardly displaced the 31 nt long duplex of GCC:CGG repeats, whereas the T-fork/(GAA:CTT)10 was displaced efficiently, as described above.
T8438	16769-16773	NN	denotes	Mcm4
T8440	16773-16774	HYPH	denotes	/
T8441	16774-16775	CD	denotes	6
T8442	16775-16776	HYPH	denotes	/
T8443	16776-16777	CD	denotes	7
T8444	16778-16784	RB	denotes	hardly
T8439	16785-16794	VBD	denotes	displaced
T8445	16795-16798	DT	denotes	the
T8447	16799-16801	CD	denotes	31
T8448	16802-16804	NN	denotes	nt
T8449	16805-16809	JJ	denotes	long
T8446	16810-16816	NN	denotes	duplex
T8450	16817-16819	IN	denotes	of
T8451	16820-16823	NN	denotes	GCC
T8453	16823-16824	:	denotes	:
T8452	16824-16827	NN	denotes	CGG
T8454	16828-16835	NNS	denotes	repeats
T8455	16835-16837	,	denotes	,
T8456	16837-16844	IN	denotes	whereas
T8458	16845-16848	DT	denotes	the
T8460	16849-16850	NN	denotes	T
T8462	16850-16851	HYPH	denotes	-
T8461	16851-16855	NN	denotes	fork
T8463	16855-16856	HYPH	denotes	/
T8464	16856-16857	-LRB-	denotes	(
T8465	16857-16860	NN	denotes	GAA
T8466	16860-16861	:	denotes	:
T8459	16861-16864	NN	denotes	CTT
T8467	16864-16865	-RRB-	denotes	)
T8468	16865-16867	CD	denotes	10
T8469	16868-16871	VBD	denotes	was
T8457	16872-16881	VBN	denotes	displaced
T8470	16882-16893	RB	denotes	efficiently
T8471	16893-16895	,	denotes	,
T8472	16895-16897	IN	denotes	as
T8473	16898-16907	VBN	denotes	described
T8474	16908-16913	RB	denotes	above
T8475	16913-16914	.	denotes	.
T8476	16914-17163	sentence	denotes	T-fork/(GCC:CIG)10 was displaced with efficiency much greater than that of T-fork/(GCC:CGG)10, indicating that the thermostability of the duplex segment, not the lack of AT base pair, is responsible for inability of Mcm4/6/7 to displace the duplex.
T8477	16915-16916	NN	denotes	T
T8479	16916-16917	HYPH	denotes	-
T8478	16917-16921	NN	denotes	fork
T8481	16921-16922	HYPH	denotes	/
T8482	16922-16923	-LRB-	denotes	(
T8483	16923-16926	NN	denotes	GCC
T8484	16926-16927	:	denotes	:
T8480	16927-16930	NN	denotes	CIG
T8486	16930-16931	-RRB-	denotes	)
T8487	16931-16933	CD	denotes	10
T8488	16934-16937	VBD	denotes	was
T8485	16938-16947	VBN	denotes	displaced
T8489	16948-16952	IN	denotes	with
T8490	16953-16963	NN	denotes	efficiency
T8491	16964-16968	RB	denotes	much
T8492	16969-16976	JJR	denotes	greater
T8493	16977-16981	IN	denotes	than
T8494	16982-16986	DT	denotes	that
T8495	16987-16989	IN	denotes	of
T8496	16990-16991	NN	denotes	T
T8498	16991-16992	HYPH	denotes	-
T8497	16992-16996	NN	denotes	fork
T8500	16996-16997	HYPH	denotes	/
T8501	16997-16998	-LRB-	denotes	(
T8502	16998-17001	NN	denotes	GCC
T8503	17001-17002	:	denotes	:
T8499	17002-17005	NN	denotes	CGG
T8504	17005-17006	-RRB-	denotes	)
T8505	17006-17008	CD	denotes	10
T8506	17008-17010	,	denotes	,
T8507	17010-17020	VBG	denotes	indicating
T8508	17021-17025	IN	denotes	that
T8510	17026-17029	DT	denotes	the
T8511	17030-17045	NN	denotes	thermostability
T8512	17046-17048	IN	denotes	of
T8513	17049-17052	DT	denotes	the
T8515	17053-17059	NN	denotes	duplex
T8514	17060-17067	NN	denotes	segment
T8516	17067-17069	,	denotes	,
T8517	17069-17072	CC	denotes	not
T8518	17073-17076	DT	denotes	the
T8519	17077-17081	NN	denotes	lack
T8520	17082-17084	IN	denotes	of
T8521	17085-17087	NN	denotes	AT
T8523	17088-17092	NN	denotes	base
T8522	17093-17097	NN	denotes	pair
T8524	17097-17099	,	denotes	,
T8509	17099-17101	VBZ	denotes	is
T8525	17102-17113	JJ	denotes	responsible
T8526	17114-17117	IN	denotes	for
T8527	17118-17127	NN	denotes	inability
T8528	17128-17130	IN	denotes	of
T8529	17131-17135	NN	denotes	Mcm4
T8530	17135-17136	HYPH	denotes	/
T8531	17136-17137	CD	denotes	6
T8532	17137-17138	HYPH	denotes	/
T8533	17138-17139	CD	denotes	7
T8534	17140-17142	TO	denotes	to
T8535	17143-17151	VB	denotes	displace
T8536	17152-17155	DT	denotes	the
T8537	17156-17162	NN	denotes	duplex
T8538	17162-17163	.	denotes	.
R2153	T3428	T3429	nmod	Mcm4,complex
R2154	T3429	T3434	nsubj	complex,interacts
R2155	T3430	T3428	punct	/,Mcm4
R2156	T3431	T3428	nummod	6,Mcm4
R2157	T3432	T3428	punct	/,Mcm4
R2158	T3433	T3428	nummod	7,Mcm4
R2159	T3435	T3434	advmod	primarily,interacts
R2160	T3436	T3434	prep	with,interacts
R2161	T3437	T3438	det	the,segment
R2162	T3438	T3436	pobj	segment,with
R2163	T3439	T3440	amod	single,stranded
R2164	T3440	T3438	amod	stranded,segment
R2165	T3441	T3440	punct	-,stranded
R2166	T3442	T3438	compound	DNA,segment
R2167	T3444	T3445	nsubj	Mcm4,binds
R2168	T3446	T3444	punct	/,Mcm4
R2169	T3447	T3444	nummod	6,Mcm4
R2170	T3448	T3444	punct	/,Mcm4
R2171	T3449	T3444	nummod	7,Mcm4
R2172	T3450	T3445	prep	to,binds
R2173	T3451	T3452	punct	‘,substrates
R2174	T3452	T3450	pobj	substrates,to
R2175	T3453	T3452	nmod	bubble,substrates
R2176	T3454	T3452	punct	’,substrates
R2177	T3455	T3452	compound	DNA,substrates
R2178	T3456	T3445	cc	and,binds
R2179	T3457	T3445	conj	unwinds,binds
R2180	T3458	T3459	det	the,segments
R2181	T3459	T3457	dobj	segments,unwinds
R2182	T3460	T3459	compound	duplex,segments
R2183	T3461	T3462	advmod	when,contains
R2184	T3462	T3457	advcl	contains,unwinds
R2185	T3463	T3464	det	the,segment
R2186	T3464	T3462	nsubj	segment,contains
R2187	T3465	T3464	amod	central,segment
R2188	T3466	T3467	amod	single,stranded
R2189	T3467	T3464	amod	stranded,segment
R2190	T3468	T3467	punct	-,stranded
R2191	T3469	T3470	compound	thymine,stretches
R2192	T3470	T3462	dobj	stretches,contains
R2193	T3471	T3472	punct	(,13
R2194	T3472	T3462	parataxis	13,contains
R2195	T3473	T3472	punct	),13
R2196	T3474	T3445	punct	.,binds
R2197	T3476	T3477	prep	In,conducted
R2198	T3478	T3476	pobj	order,In
R2199	T3479	T3480	aux	to,examine
R2200	T3480	T3478	acl	examine,order
R2201	T3481	T3482	det	the,mode
R2202	T3482	T3480	dobj	mode,examine
R2203	T3483	T3482	prep	of,mode
R2204	T3484	T3483	pobj	binding,of
R2205	T3485	T3484	prep	of,binding
R2206	T3486	T3485	pobj	Mcm4,of
R2207	T3487	T3486	punct	/,Mcm4
R2208	T3488	T3486	nummod	6,Mcm4
R2209	T3489	T3486	punct	/,Mcm4
R2210	T3490	T3486	nummod	7,Mcm4
R2211	T3491	T3484	prep	on,binding
R2212	T3492	T3493	compound	bubble,DNAs
R2213	T3493	T3491	pobj	DNAs,on
R2214	T3494	T3477	punct	", ",conducted
R2215	T3495	T3477	nsubj	we,conducted
R2216	T3496	T3497	compound	nuclease,assays
R2217	T3497	T3477	dobj	assays,conducted
R2218	T3498	T3497	compound	protection,assays
R2219	T3499	T3477	advcl	using,conducted
R2220	T3500	T3501	amod	labeled,DNAs
R2221	T3501	T3499	dobj	DNAs,using
R2222	T3502	T3503	compound	bubble,substrate
R2223	T3503	T3501	compound	substrate,DNAs
R2224	T3504	T3501	cc	and,DNAs
R2225	T3505	T3501	conj	Mcm4,DNAs
R2226	T3506	T3505	punct	/,Mcm4
R2227	T3507	T3505	nummod	6,Mcm4
R2228	T3508	T3505	punct	/,Mcm4
R2229	T3509	T3505	nummod	7,Mcm4
R2230	T3510	T3477	prep	in,conducted
R2231	T3511	T3512	det	the,presence
R2232	T3512	T3510	pobj	presence,in
R2233	T3513	T3512	prep	of,presence
R2234	T3514	T3515	nmod	ATP,S
R2235	T3515	T3513	pobj	S,of
R2236	T3516	T3515	punct	-,S
R2237	T3517	T3515	punct	γ,S
R2238	T3518	T3515	punct	-,S
R2239	T3519	T3520	dep	which,permits
R2240	T3520	T3515	relcl	permits,S
R2241	T3521	T3520	dobj	binding,permits
R2242	T3522	T3521	prep	to,binding
R2243	T3523	T3524	compound	substrate,DNA
R2244	T3524	T3522	pobj	DNA,to
R2245	T3525	T3520	cc	but,permits
R2246	T3526	T3527	aux	does,mobilize
R2247	T3527	T3520	conj	mobilize,permits
R2248	T3528	T3527	neg	not,mobilize
R2249	T3529	T3530	det	the,helicase
R2250	T3530	T3527	dobj	helicase,mobilize
R2251	T3531	T3477	punct	.,conducted
R2252	T3533	T3534	prep	At,detected
R2253	T3535	T3536	det	an,concentration
R2254	T3536	T3533	pobj	concentration,At
R2255	T3537	T3536	amod	optimum,concentration
R2256	T3538	T3536	prep	of,concentration
R2257	T3539	T3538	pobj	DNase,of
R2258	T3540	T3539	nummod	I,DNase
R2259	T3541	T3534	punct	", ",detected
R2260	T3542	T3543	amod	strong,cleavages
R2261	T3543	T3534	nsubjpass	cleavages,detected
R2262	T3544	T3534	auxpass	were,detected
R2263	T3545	T3534	prep	on,detected
R2264	T3546	T3547	det	the,regions
R2265	T3547	T3545	pobj	regions,on
R2266	T3548	T3549	nummod	21,bp
R2267	T3549	T3547	compound	bp,regions
R2268	T3550	T3547	compound	duplex,regions
R2269	T3551	T3534	prep	at,detected
R2270	T3552	T3553	det	both,ends
R2271	T3553	T3551	pobj	ends,at
R2272	T3554	T3553	prep	of,ends
R2273	T3555	T3556	det	the,substrates
R2274	T3556	T3554	pobj	substrates,of
R2275	T3557	T3534	punct	", ",detected
R2276	T3558	T3559	mark	whereas,detected
R2277	T3559	T3534	advcl	detected,detected
R2278	T3560	T3561	amod	weaker,cleavages
R2279	T3561	T3559	nsubjpass	cleavages,detected
R2280	T3562	T3559	auxpass	were,detected
R2281	T3563	T3559	prep	on,detected
R2282	T3564	T3565	det	the,region
R2283	T3565	T3563	pobj	region,on
R2284	T3566	T3565	amod	central,region
R2285	T3567	T3568	amod	single,stranded
R2286	T3568	T3565	amod	stranded,region
R2287	T3569	T3568	punct	-,stranded
R2288	T3570	T3559	punct	", ",detected
R2289	T3571	T3559	advcl	consistent,detected
R2290	T3572	T3571	prep	with,consistent
R2291	T3573	T3572	pobj	preference,with
R2292	T3574	T3573	prep	of,preference
R2293	T3575	T3574	pobj	DNase,of
R2294	T3576	T3575	nummod	I,DNase
R2295	T3577	T3573	prep	to,preference
R2296	T3578	T3579	amod	double,stranded
R2297	T3579	T3581	amod	stranded,DNA
R2298	T3580	T3579	punct	-,stranded
R2299	T3581	T3577	pobj	DNA,to
R2300	T3582	T3534	punct	.,detected
R2301	T3584	T3585	prep	With,detected
R2302	T3586	T3587	det	the,substrate
R2303	T3587	T3584	pobj	substrate,With
R2304	T3588	T3587	nmod	Bub66,substrate
R2305	T3589	T3588	punct	/,Bub66
R2306	T3590	T3588	appos	T,Bub66
R2307	T3591	T3590	punct	-,T
R2308	T3592	T3590	amod	rich,T
R2309	T3593	T3585	punct	", ",detected
R2310	T3594	T3595	amod	strong,protection
R2311	T3595	T3585	nsubjpass	protection,detected
R2312	T3596	T3585	auxpass	was,detected
R2313	T3597	T3585	prep	on,detected
R2314	T3598	T3599	det	the,regions
R2315	T3599	T3597	pobj	regions,on
R2316	T3600	T3599	amod	entire,regions
R2317	T3601	T3602	amod	single,stranded
R2318	T3602	T3599	amod	stranded,regions
R2319	T3603	T3602	punct	-,stranded
R2320	T3604	T3599	prep	of,regions
R2321	T3605	T3606	preconj	both,top
R2322	T3606	T3607	amod	top,strands
R2323	T3607	T3604	pobj	strands,of
R2324	T3608	T3606	cc	and,top
R2325	T3609	T3606	conj	bottom,top
R2326	T3610	T3585	prep	with,detected
R2327	T3611	T3612	amod	increasing,concentration
R2328	T3612	T3610	pobj	concentration,with
R2329	T3613	T3612	prep	of,concentration
R2330	T3614	T3613	pobj	Mcm4,of
R2331	T3615	T3614	punct	/,Mcm4
R2332	T3616	T3614	nummod	6,Mcm4
R2333	T3617	T3614	punct	/,Mcm4
R2334	T3618	T3614	nummod	7,Mcm4
R2335	T3619	T3585	punct	", ",detected
R2336	T3620	T3585	advcl	consistent,detected
R2337	T3621	T3620	prep	with,consistent
R2338	T3622	T3623	amod	high,affinity
R2339	T3623	T3621	pobj	affinity,with
R2340	T3624	T3623	prep	of,affinity
R2341	T3625	T3624	pobj	Mcm4,of
R2342	T3626	T3625	punct	/,Mcm4
R2343	T3627	T3625	nummod	6,Mcm4
R2344	T3628	T3625	punct	/,Mcm4
R2345	T3629	T3625	nummod	7,Mcm4
R2346	T3630	T3623	prep	to,affinity
R2347	T3631	T3632	nmod	T,sequences
R2348	T3632	T3630	pobj	sequences,to
R2349	T3633	T3631	punct	-,T
R2350	T3634	T3631	amod	rich,T
R2351	T3635	T3632	compound	bubble,sequences
R2352	T3636	T3637	punct	(,1A
R2353	T3637	T3585	parataxis	1A,detected
R2354	T3638	T3637	compound	Figure,1A
R2355	T3639	T3637	punct	),1A
R2356	T3640	T3585	punct	.,detected
R2357	T3642	T3643	amod	Similar,protection
R2358	T3643	T3648	nsubjpass	protection,detected
R2359	T3644	T3642	punct	", ",Similar
R2360	T3645	T3642	cc	but,Similar
R2361	T3646	T3647	advmod	less,significant
R2362	T3647	T3642	conj	significant,Similar
R2363	T3649	T3648	auxpass	was,detected
R2364	T3650	T3648	prep	on,detected
R2365	T3651	T3652	det	the,segment
R2366	T3652	T3650	pobj	segment,on
R2367	T3653	T3654	amod	single,stranded
R2368	T3654	T3652	amod	stranded,segment
R2369	T3655	T3654	punct	-,stranded
R2370	T3656	T3652	compound	DNA,segment
R2371	T3657	T3652	prep	with,segment
R2372	T3658	T3659	compound	P1,nucleases
R2373	T3659	T3657	pobj	nucleases,with
R2374	T3660	T3661	punct	(,1A
R2375	T3661	T3652	parataxis	1A,segment
R2376	T3662	T3661	compound	Figure,1A
R2377	T3663	T3661	punct	),1A
R2378	T3664	T3652	punct	", ",segment
R2379	T3665	T3666	dep	which,is
R2380	T3666	T3652	relcl	is,segment
R2381	T3667	T3666	acomp	specific,is
R2382	T3668	T3667	prep	to,specific
R2383	T3669	T3670	amod	single,stranded
R2384	T3670	T3672	amod	stranded,DNA
R2385	T3671	T3670	punct	-,stranded
R2386	T3672	T3668	pobj	DNA,to
R2387	T3673	T3648	punct	.,detected
R2388	T3675	T3676	det	The,results
R2389	T3676	T3677	nsubj	results,indicate
R2390	T3678	T3679	mark	that,loaded
R2391	T3679	T3677	ccomp	loaded,indicate
R2392	T3680	T3681	det	the,complex
R2393	T3681	T3679	nsubjpass	complex,loaded
R2394	T3682	T3681	compound	Mcm,complex
R2395	T3683	T3679	auxpass	is,loaded
R2396	T3684	T3679	prep	onto,loaded
R2397	T3685	T3686	compound	bubble,DNA
R2398	T3686	T3684	pobj	DNA,onto
R2399	T3687	T3679	prep	through,loaded
R2400	T3688	T3687	pobj	binding,through
R2401	T3689	T3688	prep	to,binding
R2402	T3690	T3691	det	both,strands
R2403	T3691	T3689	pobj	strands,to
R2404	T3692	T3691	prep	of,strands
R2405	T3693	T3694	det	the,region
R2406	T3694	T3692	pobj	region,of
R2407	T3695	T3696	amod	single,stranded
R2408	T3696	T3694	amod	stranded,region
R2409	T3697	T3696	punct	-,stranded
R2410	T3698	T3677	punct	.,indicate
R2411	T3700	T3701	amod	Careful,examination
R2412	T3701	T3702	nsubj	examination,indicated
R2413	T3703	T3701	prep	of,examination
R2414	T3704	T3705	nmod	Mcm4,footprints
R2415	T3705	T3703	pobj	footprints,of
R2416	T3706	T3704	punct	/,Mcm4
R2417	T3707	T3704	nummod	6,Mcm4
R2418	T3708	T3704	punct	/,Mcm4
R2419	T3709	T3704	nummod	7,Mcm4
R2420	T3710	T3705	prep	on,footprints
R2421	T3711	T3712	det	a,bubble
R2422	T3712	T3710	pobj	bubble,on
R2423	T3713	T3714	amod	strong,protection
R2424	T3714	T3702	dobj	protection,indicated
R2425	T3715	T3714	prep	on,protection
R2426	T3716	T3717	det	the,half
R2427	T3717	T3715	pobj	half,on
R2428	T3718	T3717	nummod	5,half
R2429	T3719	T3718	punct	′,5
R2430	T3720	T3717	punct	-,half
R2431	T3721	T3717	prep	of,half
R2432	T3722	T3723	det	the,strand
R2433	T3723	T3721	pobj	strand,of
R2434	T3724	T3723	amod	top,strand
R2435	T3725	T3714	cc	and,protection
R2436	T3726	T3727	amod	moderate,protection
R2437	T3727	T3714	conj	protection,protection
R2438	T3728	T3727	prep	on,protection
R2439	T3729	T3730	det	the,half
R2440	T3730	T3728	pobj	half,on
R2441	T3731	T3730	amod	remaining,half
R2442	T3732	T3730	nummod	3,half
R2443	T3733	T3732	punct	′,3
R2444	T3734	T3730	punct	-,half
R2445	T3735	T3728	punct	", ",on
R2446	T3736	T3737	advmod	as,as
R2447	T3737	T3728	cc	as,on
R2448	T3738	T3737	advmod	well,as
R2449	T3739	T3728	conj	on,on
R2450	T3740	T3741	amod	several,segment
R2451	T3741	T3739	pobj	segment,on
R2452	T3742	T3743	compound	base,pair
R2453	T3743	T3741	compound	pair,segment
R2454	T3744	T3743	punct	-,pair
R2455	T3745	T3741	compound	duplex,segment
R2456	T3746	T3741	amod	adjacent,segment
R2457	T3747	T3746	prep	to,adjacent
R2458	T3748	T3749	det	the,point
R2459	T3749	T3747	pobj	point,to
R2460	T3750	T3749	compound	branch,point
R2461	T3751	T3752	punct	(,1A
R2462	T3752	T3739	parataxis	1A,on
R2463	T3753	T3752	compound	Figure,1A
R2464	T3754	T3752	cc	and,1A
R2465	T3755	T3752	conj	data,1A
R2466	T3756	T3757	neg	not,shown
R2467	T3757	T3755	acl	shown,data
R2468	T3758	T3752	punct	),1A
R2469	T3759	T3702	punct	.,indicated
R2470	T3761	T3762	det	A,pattern
R2471	T3762	T3764	nsubjpass	pattern,observed
R2472	T3763	T3762	amod	similar,pattern
R2473	T3765	T3762	prep	of,pattern
R2474	T3766	T3765	pobj	protection,of
R2475	T3767	T3764	auxpass	was,observed
R2476	T3768	T3764	advmod	also,observed
R2477	T3769	T3764	prep	on,observed
R2478	T3770	T3771	det	the,strand
R2479	T3771	T3769	pobj	strand,on
R2480	T3772	T3771	amod	bottom,strand
R2481	T3773	T3764	punct	.,observed
R2482	T3775	T3776	nsubj	This,suggests
R2483	T3777	T3778	mark	that,bind
R2484	T3778	T3776	ccomp	bind,suggests
R2485	T3779	T3778	nsubj	Mcm4,bind
R2486	T3780	T3779	punct	/,Mcm4
R2487	T3781	T3779	nummod	6,Mcm4
R2488	T3782	T3779	punct	/,Mcm4
R2489	T3783	T3779	nummod	7,Mcm4
R2490	T3784	T3778	aux	may,bind
R2491	T3785	T3778	prep	to,bind
R2492	T3786	T3787	det	a,substrate
R2493	T3787	T3785	pobj	substrate,to
R2494	T3788	T3787	compound	replication,substrate
R2495	T3789	T3787	compound	bubble,substrate
R2496	T3790	T3787	prep	with,substrate
R2497	T3791	T3792	det	a,symmetry
R2498	T3792	T3790	pobj	symmetry,with
R2499	T3793	T3792	nummod	2-fold,symmetry
R2500	T3794	T3778	prep	as,bind
R2501	T3795	T3796	det	a,hexamer
R2502	T3796	T3794	pobj	hexamer,as
R2503	T3797	T3796	amod	double,hexamer
R2504	T3798	T3799	punct	(,1A
R2505	T3799	T3796	parataxis	1A,hexamer
R2506	T3800	T3799	compound	Figure,1A
R2507	T3801	T3799	punct	", ",1A
R2508	T3802	T3799	advcl	see,1A
R2509	T3803	T3804	det	the,scheme
R2510	T3804	T3802	dobj	scheme,see
R2511	T3805	T3804	amod	lower,scheme
R2512	T3806	T3799	punct	),1A
R2513	T3807	T3778	punct	", ",bind
R2514	T3808	T3778	prep	in,bind
R2515	T3809	T3810	det	a,manner
R2516	T3810	T3808	pobj	manner,in
R2517	T3811	T3810	amod	similar,manner
R2518	T3812	T3811	prep	to,similar
R2519	T3813	T3814	compound	SV40,antigen
R2520	T3814	T3812	pobj	antigen,to
R2521	T3815	T3814	compound	T,antigen
R2522	T3816	T3814	punct	-,antigen
R2523	T3817	T3818	punct	(,21
R2524	T3818	T3778	parataxis	21,bind
R2525	T3819	T3818	punct	),21
R2526	T3820	T3776	punct	.,suggests
R2527	T3822	T3823	compound	Nuclease,protection
R2528	T3823	T3824	compound	protection,assays
R2529	T3824	T3825	nsubjpass	assays,conducted
R2530	T3826	T3825	auxpass	were,conducted
R2531	T3827	T3825	advmod	also,conducted
R2532	T3828	T3825	prep	on,conducted
R2533	T3829	T3830	det	a,structure
R2534	T3830	T3828	pobj	structure,on
R2535	T3831	T3832	npadvmod	T,tailed
R2536	T3832	T3830	amod	tailed,structure
R2537	T3833	T3832	punct	-,tailed
R2538	T3834	T3835	compound	Y,fork
R2539	T3835	T3830	compound	fork,structure
R2540	T3836	T3835	punct	-,fork
R2541	T3837	T3838	punct	(,1B
R2542	T3838	T3825	parataxis	1B,conducted
R2543	T3839	T3838	compound	Figure,1B
R2544	T3840	T3838	punct	),1B
R2545	T3841	T3825	punct	.,conducted
R2546	T3843	T3844	preconj	Not,region
R2547	T3844	T3851	nsubjpass	region,protected
R2548	T3845	T3843	advmod	only,Not
R2549	T3846	T3844	det	the,region
R2550	T3847	T3848	amod	single,stranded
R2551	T3848	T3844	amod	stranded,region
R2552	T3849	T3848	punct	-,stranded
R2553	T3850	T3844	compound	tail,region
R2554	T3852	T3844	cc	but,region
R2555	T3853	T3852	advmod	also,but
R2556	T3854	T3855	det	the,DNA
R2557	T3855	T3844	conj	DNA,region
R2558	T3856	T3857	nummod	7,bp
R2559	T3857	T3855	compound	bp,DNA
R2560	T3858	T3855	compound	duplex,DNA
R2561	T3859	T3855	amod	adjacent,DNA
R2562	T3860	T3859	prep	to,adjacent
R2563	T3861	T3862	det	the,junction
R2564	T3862	T3860	pobj	junction,to
R2565	T3863	T3862	nmod	duplex,junction
R2566	T3864	T3863	punct	-,duplex
R2567	T3865	T3863	prep	to,duplex
R2568	T3866	T3865	punct	-,to
R2569	T3867	T3868	amod	single,strand
R2570	T3868	T3865	pobj	strand,to
R2571	T3869	T3868	punct	-,strand
R2572	T3870	T3851	auxpass	were,protected
R2573	T3871	T3851	agent	by,protected
R2574	T3872	T3873	compound	Mcm,binding
R2575	T3873	T3871	pobj	binding,by
R2576	T3874	T3873	prep	from,binding
R2577	T3875	T3876	compound	DNaseI,digestion
R2578	T3876	T3874	pobj	digestion,from
R2579	T3877	T3851	punct	.,protected
R2580	T3879	T3880	det	These,results
R2581	T3880	T3881	nsubj	results,indicate
R2582	T3882	T3883	mark	that,interacts
R2583	T3883	T3881	advcl	interacts,indicate
R2584	T3884	T3885	nmod	Mcm4,protein
R2585	T3885	T3883	nsubj	protein,interacts
R2586	T3886	T3884	punct	/,Mcm4
R2587	T3887	T3884	nummod	6,Mcm4
R2588	T3888	T3884	punct	/,Mcm4
R2589	T3889	T3884	nummod	7,Mcm4
R2590	T3890	T3883	advmod	primarily,interacts
R2591	T3891	T3883	prep	with,interacts
R2592	T3892	T3893	amod	single,stranded
R2593	T3893	T3895	amod	stranded,region
R2594	T3894	T3893	punct	-,stranded
R2595	T3895	T3891	pobj	region,with
R2596	T3896	T3895	compound	DNA,region
R2597	T3897	T3883	cc	and,interacts
R2598	T3898	T3899	mark	that,spans
R2599	T3899	T3883	conj	spans,interacts
R2600	T3900	T3901	det	the,interaction
R2601	T3901	T3899	nsubj	interaction,spans
R2602	T3902	T3899	advmod	also,spans
R2603	T3903	T3904	det	the,segments
R2604	T3904	T3899	dobj	segments,spans
R2605	T3905	T3904	compound	duplex,segments
R2606	T3906	T3904	prep	near,segments
R2607	T3907	T3908	det	the,branchpoint
R2608	T3908	T3906	pobj	branchpoint,near
R2609	T3909	T3908	prep	of,branchpoint
R2610	T3910	T3911	det	the,fork
R2611	T3911	T3909	pobj	fork,of
R2612	T3912	T3911	compound	replication,fork
R2613	T3913	T3881	punct	.,indicate
R2617	T4077	T4078	nsubj	Formation,facilitates
R2618	T4079	T4077	prep	of,Formation
R2619	T4080	T4081	amod	double,hexameric
R2620	T4081	T4082	amod	hexameric,complexes
R2621	T4082	T4079	pobj	complexes,of
R2622	T4083	T4082	nmod	Mcm4,complexes
R2623	T4084	T4083	punct	/,Mcm4
R2624	T4085	T4083	nummod	6,Mcm4
R2625	T4086	T4083	punct	/,Mcm4
R2626	T4087	T4083	nummod	7,Mcm4
R2627	T4088	T4089	compound	helicase,actions
R2628	T4089	T4078	dobj	actions,facilitates
R2629	T4090	T4078	prep	on,facilitates
R2630	T4091	T4092	compound	bubble,substrates
R2631	T4092	T4090	pobj	substrates,on
R2632	T4094	T4095	det	The,results
R2633	T4095	T4097	nsubj	results,suggest
R2634	T4096	T4095	amod	above,results
R2635	T4098	T4099	mark	that,generates
R2636	T4099	T4097	ccomp	generates,suggest
R2637	T4100	T4099	nsubj	Mcm4,generates
R2638	T4101	T4100	punct	/,Mcm4
R2639	T4102	T4100	nummod	6,Mcm4
R2640	T4103	T4100	punct	/,Mcm4
R2641	T4104	T4100	nummod	7,Mcm4
R2642	T4105	T4106	det	a,complex
R2643	T4106	T4099	dobj	complex,generates
R2644	T4107	T4108	amod	double,hexameric
R2645	T4108	T4106	amod	hexameric,complex
R2646	T4109	T4099	prep	on,generates
R2647	T4110	T4111	det	a,bubble
R2648	T4111	T4109	pobj	bubble,on
R2649	T4112	T4099	punct	", ",generates
R2650	T4113	T4114	det	each,hexamer
R2651	T4114	T4115	dep	hexamer,makes
R2652	T4115	T4099	ccomp	makes,generates
R2653	T4116	T4114	prep	of,hexamer
R2654	T4117	T4116	pobj	which,of
R2655	T4118	T4119	amod	symmetric,contact
R2656	T4119	T4115	dobj	contact,makes
R2657	T4120	T4119	prep	with,contact
R2658	T4121	T4122	det	the,segment
R2659	T4122	T4120	pobj	segment,with
R2660	T4123	T4124	npadvmod	5,proximal
R2661	T4124	T4122	amod	proximal,segment
R2662	T4125	T4123	punct	′,5
R2663	T4126	T4124	punct	-,proximal
R2664	T4127	T4122	amod	central,segment
R2665	T4128	T4129	amod	single,stranded
R2666	T4129	T4122	amod	stranded,segment
R2667	T4130	T4129	punct	-,stranded
R2668	T4131	T4097	punct	.,suggest
R2669	T4133	T4134	nsubj	This,predicts
R2670	T4135	T4136	mark	that,needs
R2671	T4136	T4134	ccomp	needs,predicts
R2672	T4137	T4138	det	the,segment
R2673	T4138	T4136	nsubj	segment,needs
R2674	T4139	T4140	amod	single,stranded
R2675	T4140	T4138	amod	stranded,segment
R2676	T4141	T4140	punct	-,stranded
R2677	T4142	T4143	aux	to,be
R2678	T4143	T4136	xcomp	be,needs
R2679	T4144	T4143	prep	of,be
R2680	T4145	T4146	det	a,length
R2681	T4146	T4144	pobj	length,of
R2682	T4147	T4146	amod	sufficient,length
R2683	T4148	T4143	prep	in,be
R2684	T4149	T4148	pobj	order,in
R2685	T4150	T4151	aux	to,accommodate
R2686	T4151	T4149	acl	accommodate,order
R2687	T4152	T4153	det	the,complex
R2688	T4153	T4151	dobj	complex,accommodate
R2689	T4154	T4153	compound	Mcm,complex
R2690	T4155	T4134	punct	.,predicts
R2691	T4157	T4158	prep	In,showed
R2692	T4159	T4157	pobj	fact,In
R2693	T4160	T4158	punct	", ",showed
R2694	T4161	T4158	nsubj	we,showed
R2695	T4162	T4158	advmod	previously,showed
R2696	T4163	T4164	mark	that,forms
R2697	T4164	T4158	ccomp	forms,showed
R2698	T4165	T4166	det	the,Mcm4
R2699	T4166	T4164	nsubj	Mcm4,forms
R2700	T4167	T4166	punct	/,Mcm4
R2701	T4168	T4166	nummod	6,Mcm4
R2702	T4169	T4166	punct	/,Mcm4
R2703	T4170	T4166	nummod	7,Mcm4
R2704	T4171	T4164	advmod	mainly,forms
R2705	T4172	T4173	det	a,complex
R2706	T4173	T4164	dobj	complex,forms
R2707	T4174	T4175	amod	single,hexameric
R2708	T4175	T4173	amod	hexameric,complex
R2709	T4176	T4173	prep	on,complex
R2710	T4177	T4176	pobj	bubble,on
R2711	T4178	T4177	punct	-,bubble
R2712	T4179	T4177	nummod	20,bubble
R2713	T4180	T4177	punct	(,bubble
R2714	T4181	T4182	det	a,substrate
R2715	T4182	T4177	appos	substrate,bubble
R2716	T4183	T4182	compound	bubble,substrate
R2717	T4184	T4182	prep	with,substrate
R2718	T4185	T4186	nummod	20,nt
R2719	T4186	T4187	npadvmod	nt,long
R2720	T4187	T4188	amod	long,segment
R2721	T4188	T4184	pobj	segment,with
R2722	T4189	T4188	amod	central,segment
R2723	T4190	T4191	amod	single,stranded
R2724	T4191	T4188	amod	stranded,segment
R2725	T4192	T4191	punct	-,stranded
R2726	T4193	T4173	punct	),complex
R2727	T4194	T4173	cc	and,complex
R2728	T4195	T4196	det	a,complex
R2729	T4196	T4173	conj	complex,complex
R2730	T4197	T4198	amod	double,hexameric
R2731	T4198	T4196	amod	hexameric,complex
R2732	T4199	T4196	prep	on,complex
R2733	T4200	T4201	nmod	bubble,DNA
R2734	T4201	T4199	pobj	DNA,on
R2735	T4202	T4200	punct	-,bubble
R2736	T4203	T4200	nummod	60,bubble
R2737	T4204	T4205	punct	(,13
R2738	T4205	T4196	parataxis	13,complex
R2739	T4206	T4205	punct	),13
R2740	T4207	T4158	punct	.,showed
R2741	T4209	T4210	prep	In,constructed
R2742	T4211	T4209	pobj	order,In
R2743	T4212	T4213	aux	to,determine
R2744	T4213	T4211	acl	determine,order
R2745	T4214	T4215	det	the,requirement
R2746	T4215	T4213	dobj	requirement,determine
R2747	T4216	T4215	amod	minimum,requirement
R2748	T4217	T4215	prep	for,requirement
R2749	T4218	T4219	amod	double,hexamer
R2750	T4219	T4221	compound	hexamer,formation
R2751	T4220	T4219	punct	-,hexamer
R2752	T4221	T4217	pobj	formation,for
R2753	T4222	T4210	punct	", ",constructed
R2754	T4223	T4224	det	a,set
R2755	T4224	T4210	nsubjpass	set,constructed
R2756	T4225	T4224	prep	of,set
R2757	T4226	T4227	amod	synthetic,substrates
R2758	T4227	T4225	pobj	substrates,of
R2759	T4228	T4229	npadvmod	bubble,like
R2760	T4229	T4227	amod	like,substrates
R2761	T4230	T4229	punct	-,like
R2762	T4231	T4227	punct	(,substrates
R2763	T4232	T4233	compound	Bub,T10
R2764	T4233	T4227	appos	T10,substrates
R2765	T4234	T4233	punct	-,T10
R2766	T4235	T4233	punct	", ",T10
R2767	T4236	T4237	compound	Bub,T20
R2768	T4237	T4233	conj	T20,T10
R2769	T4238	T4237	punct	-,T20
R2770	T4239	T4237	punct	", ",T20
R2771	T4240	T4241	compound	Bub,T30
R2772	T4241	T4237	conj	T30,T20
R2773	T4242	T4241	punct	-,T30
R2774	T4243	T4241	punct	", ",T30
R2775	T4244	T4245	compound	Bub,T40
R2776	T4245	T4241	conj	T40,T30
R2777	T4246	T4245	punct	-,T40
R2778	T4247	T4245	cc	and,T40
R2779	T4248	T4249	compound	Bub,T50
R2780	T4249	T4245	conj	T50,T40
R2781	T4250	T4249	punct	-,T50
R2782	T4251	T4227	punct	),substrates
R2783	T4252	T4253	dep	that,differed
R2784	T4253	T4227	relcl	differed,substrates
R2785	T4254	T4253	prep	in,differed
R2786	T4255	T4256	det	the,length
R2787	T4256	T4254	pobj	length,in
R2788	T4257	T4256	prep	of,length
R2789	T4258	T4259	det	the,segment
R2790	T4259	T4257	pobj	segment,of
R2791	T4260	T4259	amod	central,segment
R2792	T4261	T4259	amod	unpaired,segment
R2793	T4262	T4210	auxpass	were,constructed
R2794	T4263	T4210	punct	", ",constructed
R2795	T4264	T4210	cc	and,constructed
R2796	T4265	T4266	det	the,activities
R2797	T4266	T4270	nsubjpass	activities,examined
R2798	T4267	T4266	nmod	binding,activities
R2799	T4268	T4267	cc	and,binding
R2800	T4269	T4267	conj	helicase,binding
R2801	T4270	T4210	conj	examined,constructed
R2802	T4271	T4266	prep	of,activities
R2803	T4272	T4271	pobj	Mcm4,of
R2804	T4273	T4272	punct	/,Mcm4
R2805	T4274	T4272	nummod	6,Mcm4
R2806	T4275	T4272	punct	/,Mcm4
R2807	T4276	T4272	nummod	7,Mcm4
R2808	T4277	T4266	prep	on,activities
R2809	T4278	T4279	det	these,substrates
R2810	T4279	T4277	pobj	substrates,on
R2811	T4280	T4270	auxpass	were,examined
R2812	T4281	T4210	punct	.,constructed
R2813	T4283	T4284	nsubjpass	Mcm4,incubated
R2814	T4285	T4283	punct	/,Mcm4
R2815	T4286	T4283	nummod	6,Mcm4
R2816	T4287	T4283	punct	/,Mcm4
R2817	T4288	T4283	nummod	7,Mcm4
R2818	T4289	T4284	auxpass	was,incubated
R2819	T4290	T4284	prep	with,incubated
R2820	T4291	T4292	det	a,substrate
R2821	T4292	T4290	pobj	substrate,with
R2822	T4293	T4292	amod	radiolabeled,substrate
R2823	T4294	T4292	amod	synthetic,substrate
R2824	T4295	T4292	compound	bubble,substrate
R2825	T4296	T4284	prep	in,incubated
R2826	T4297	T4298	det	the,presence
R2827	T4298	T4296	pobj	presence,in
R2828	T4299	T4298	prep	of,presence
R2829	T4300	T4301	nummod	0.5,mM
R2830	T4301	T4302	nmod	mM,S
R2831	T4302	T4299	pobj	S,of
R2832	T4303	T4302	nmod	ATP,S
R2833	T4304	T4302	punct	-,S
R2834	T4305	T4302	punct	γ,S
R2835	T4306	T4302	punct	-,S
R2836	T4307	T4308	aux	to,allow
R2837	T4308	T4284	advcl	allow,incubated
R2838	T4309	T4310	compound	complex,formation
R2839	T4310	T4308	dobj	formation,allow
R2840	T4311	T4284	punct	.,incubated
R2841	T4313	T4314	det	A,half
R2842	T4314	T4315	nsubjpass	half,analyzed
R2843	T4316	T4314	prep	of,half
R2844	T4317	T4318	det	these,mixtures
R2845	T4318	T4316	pobj	mixtures,of
R2846	T4319	T4318	compound	reaction,mixtures
R2847	T4320	T4315	auxpass	were,analyzed
R2848	T4321	T4315	prep	for,analyzed
R2849	T4322	T4323	compound	DNA,binding
R2850	T4323	T4321	pobj	binding,for
R2851	T4324	T4315	prep	in,analyzed
R2852	T4325	T4326	compound	gel,shift
R2853	T4326	T4327	compound	shift,assays
R2854	T4327	T4324	pobj	assays,in
R2855	T4328	T4329	punct	(,2A
R2856	T4329	T4315	parataxis	2A,analyzed
R2857	T4330	T4329	compound	Figure,2A
R2858	T4331	T4329	punct	),2A
R2859	T4332	T4315	punct	", ",analyzed
R2860	T4333	T4315	cc	and,analyzed
R2861	T4334	T4335	det	the,remainder
R2862	T4335	T4336	nsubjpass	remainder,incubated
R2863	T4336	T4315	conj	incubated,analyzed
R2864	T4337	T4336	auxpass	was,incubated
R2865	T4338	T4336	advmod	further,incubated
R2866	T4339	T4336	prep	in,incubated
R2867	T4340	T4341	det	the,presence
R2868	T4341	T4339	pobj	presence,in
R2869	T4342	T4341	prep	of,presence
R2870	T4343	T4344	nummod	10,mM
R2871	T4344	T4345	compound	mM,ATP
R2872	T4345	T4342	pobj	ATP,of
R2873	T4346	T4347	aux	to,measure
R2874	T4347	T4336	advcl	measure,incubated
R2875	T4348	T4349	compound	DNA,helicase
R2876	T4349	T4350	compound	helicase,activity
R2877	T4350	T4347	dobj	activity,measure
R2878	T4351	T4352	punct	(,2B
R2879	T4352	T4347	parataxis	2B,measure
R2880	T4353	T4352	compound	Figure,2B
R2881	T4354	T4352	punct	),2B
R2882	T4355	T4336	punct	.,incubated
R2883	T4357	T4358	nsubj	Bub,generated
R2884	T4359	T4357	punct	-,Bub
R2885	T4360	T4357	nummod	10,Bub
R2886	T4361	T4362	advmod	only,level
R2887	T4362	T4358	dobj	level,generated
R2888	T4363	T4362	det	a,level
R2889	T4364	T4362	amod	low,level
R2890	T4365	T4362	prep	of,level
R2891	T4366	T4365	pobj	complexes,of
R2892	T4367	T4366	prep	with,complexes
R2893	T4368	T4367	pobj	Mcm4,with
R2894	T4369	T4368	punct	/,Mcm4
R2895	T4370	T4368	nummod	6,Mcm4
R2896	T4371	T4368	punct	/,Mcm4
R2897	T4372	T4368	nummod	7,Mcm4
R2898	T4373	T4358	punct	", ",generated
R2899	T4374	T4358	cc	and,generated
R2900	T4375	T4376	nsubj	Bub,generated
R2901	T4376	T4358	conj	generated,generated
R2902	T4377	T4375	punct	-,Bub
R2903	T4378	T4375	nummod	20,Bub
R2904	T4379	T4376	advmod	primarily,generated
R2905	T4380	T4381	det	a,form
R2906	T4381	T4376	dobj	form,generated
R2907	T4382	T4381	amod	single,form
R2908	T4383	T4384	npadvmod	mobility,shifted
R2909	T4384	T4381	amod	shifted,form
R2910	T4385	T4384	punct	-,shifted
R2911	T4386	T4381	punct	", ",form
R2912	T4387	T4388	dep	which,contain
R2913	T4388	T4381	relcl	contain,form
R2914	T4389	T4388	aux	may,contain
R2915	T4390	T4391	det	a,hexamer
R2916	T4391	T4388	dobj	hexamer,contain
R2917	T4392	T4391	amod	single,hexamer
R2918	T4393	T4391	prep	of,hexamer
R2919	T4394	T4393	pobj	Mcm4,of
R2920	T4395	T4394	punct	/,Mcm4
R2921	T4396	T4394	nummod	6,Mcm4
R2922	T4397	T4394	punct	/,Mcm4
R2923	T4398	T4394	nummod	7,Mcm4
R2924	T4399	T4376	punct	.,generated
R2925	T4401	T4402	prep	On,generated
R2926	T4403	T4401	pobj	Bub,On
R2927	T4404	T4403	punct	-,Bub
R2928	T4405	T4403	nummod	30,Bub
R2929	T4406	T4402	punct	", ",generated
R2930	T4407	T4408	amod	significant,amount
R2931	T4408	T4402	nsubjpass	amount,generated
R2932	T4409	T4408	prep	of,amount
R2933	T4410	T4409	pobj	complexes,of
R2934	T4411	T4410	acl	containing,complexes
R2935	T4412	T4413	det	a,hexamer
R2936	T4413	T4411	dobj	hexamer,containing
R2937	T4414	T4413	amod	double,hexamer
R2938	T4415	T4402	auxpass	were,generated
R2939	T4416	T4402	prep	in,generated
R2940	T4417	T4416	pobj	addition,in
R2941	T4418	T4417	prep	to,addition
R2942	T4419	T4418	pobj	those,to
R2943	T4420	T4419	acl	containing,those
R2944	T4421	T4422	det	a,hexamer
R2945	T4422	T4420	dobj	hexamer,containing
R2946	T4423	T4422	amod	single,hexamer
R2947	T4424	T4425	punct	(,2A
R2948	T4425	T4402	parataxis	2A,generated
R2949	T4426	T4425	compound	Figure,2A
R2950	T4427	T4425	punct	),2A
R2951	T4428	T4429	punct	(,21
R2952	T4429	T4402	parataxis	21,generated
R2953	T4430	T4429	nummod	13,21
R2954	T4431	T4429	punct	",",21
R2955	T4432	T4429	punct	),21
R2956	T4433	T4402	punct	.,generated
R2957	T4435	T4436	det	The,amounts
R2958	T4436	T4437	nsubj	amounts,increased
R2959	T4438	T4436	prep	of,amounts
R2960	T4439	T4440	amod	double,hexamer
R2961	T4440	T4441	compound	hexamer,complexes
R2962	T4441	T4438	pobj	complexes,of
R2963	T4442	T4437	prep	on,increased
R2964	T4443	T4444	compound	Bub,T40
R2965	T4444	T4442	pobj	T40,on
R2966	T4445	T4444	punct	-,T40
R2967	T4446	T4444	cc	and,T40
R2968	T4447	T4448	compound	Bub,T50
R2969	T4448	T4444	conj	T50,T40
R2970	T4449	T4448	punct	-,T50
R2971	T4450	T4437	prep	with,increased
R2972	T4451	T4452	amod	concomitant,decrease
R2973	T4452	T4450	pobj	decrease,with
R2974	T4453	T4452	prep	of,decrease
R2975	T4454	T4455	amod	single,hexamer
R2976	T4455	T4456	compound	hexamer,complexes
R2977	T4456	T4453	pobj	complexes,of
R2978	T4457	T4458	punct	(,2A
R2979	T4458	T4437	parataxis	2A,increased
R2980	T4459	T4458	compound	Figure,2A
R2981	T4460	T4458	punct	),2A
R2982	T4461	T4437	punct	.,increased
R2983	T4463	T4464	det	The,results
R2984	T4464	T4465	nsubj	results,indicate
R2985	T4466	T4467	mark	that,required
R2986	T4467	T4465	ccomp	required,indicate
R2987	T4468	T4469	det	a,segment
R2988	T4469	T4467	nsubjpass	segment,required
R2989	T4470	T4471	amod	single,stranded
R2990	T4471	T4469	amod	stranded,segment
R2991	T4472	T4471	punct	-,stranded
R2992	T4473	T4469	prep	of,segment
R2993	T4474	T4475	advmod	at,least
R2994	T4475	T4476	advmod	least,40
R2995	T4476	T4477	nummod	40,nt
R2996	T4477	T4473	pobj	nt,of
R2997	T4478	T4477	amod	long,nt
R2998	T4479	T4467	auxpass	is,required
R2999	T4480	T4467	prep	for,required
R3000	T4481	T4482	amod	efficient,loading
R3001	T4482	T4480	pobj	loading,for
R3002	T4483	T4482	prep	of,loading
R3003	T4484	T4485	det	the,helicase
R3004	T4485	T4483	pobj	helicase,of
R3005	T4486	T4487	amod	double,hexameric
R3006	T4487	T4485	amod	hexameric,helicase
R3007	T4488	T4487	punct	-,hexameric
R3008	T4489	T4485	compound	Mcm,helicase
R3009	T4490	T4482	prep	on,loading
R3010	T4491	T4492	det	a,substrate
R3011	T4492	T4490	pobj	substrate,on
R3012	T4493	T4492	compound	bubble,substrate
R3013	T4494	T4465	punct	.,indicate
R3014	T4496	T4497	prep	In,show
R3015	T4498	T4499	compound	DNA,helicase
R3016	T4499	T4500	compound	helicase,assays
R3017	T4500	T4496	pobj	assays,In
R3018	T4501	T4497	punct	", ",show
R3019	T4502	T4503	nmod	Mcm4,complex
R3020	T4503	T4497	nsubj	complex,show
R3021	T4504	T4502	punct	/,Mcm4
R3022	T4505	T4502	nummod	6,Mcm4
R3023	T4506	T4502	punct	/,Mcm4
R3024	T4507	T4502	nummod	7,Mcm4
R3025	T4508	T4497	aux	did,show
R3026	T4509	T4497	neg	not,show
R3027	T4510	T4511	compound	helicase,activity
R3028	T4511	T4497	dobj	activity,show
R3029	T4512	T4497	prep	on,show
R3030	T4513	T4514	preconj	either,Bub
R3031	T4514	T4515	nmod	Bub,substrate
R3032	T4515	T4512	pobj	substrate,on
R3033	T4516	T4514	punct	-,Bub
R3034	T4517	T4514	nummod	10,Bub
R3035	T4518	T4514	cc	or,Bub
R3036	T4519	T4514	conj	Bub,Bub
R3037	T4520	T4519	punct	-,Bub
R3038	T4521	T4519	nummod	20,Bub
R3039	T4522	T4497	punct	.,show
R3040	T4524	T4525	det	The,efficiency
R3041	T4525	T4526	nsubj	efficiency,improved
R3042	T4527	T4525	prep	of,efficiency
R3043	T4528	T4527	pobj	displacement,of
R3044	T4529	T4526	advmod	slightly,improved
R3045	T4530	T4531	mark	as,increased
R3046	T4531	T4526	advcl	increased,improved
R3047	T4532	T4533	det	the,length
R3048	T4533	T4531	nsubj	length,increased
R3049	T4534	T4533	prep	of,length
R3050	T4535	T4536	det	the,segment
R3051	T4536	T4534	pobj	segment,of
R3052	T4537	T4538	amod	single,stranded
R3053	T4538	T4536	amod	stranded,segment
R3054	T4539	T4538	punct	-,stranded
R3055	T4540	T4541	quantmod	up,30
R3056	T4541	T4543	nummod	30,nt
R3057	T4542	T4541	quantmod	to,30
R3058	T4543	T4531	npadvmod	nt,increased
R3059	T4544	T4526	punct	.,improved
R3060	T4546	T4547	prep	On,observed
R3061	T4548	T4549	compound	Bub,T40
R3062	T4549	T4546	pobj	T40,On
R3063	T4550	T4549	punct	-,T40
R3064	T4551	T4549	cc	and,T40
R3065	T4552	T4553	compound	Bub,T50
R3066	T4553	T4549	conj	T50,T40
R3067	T4554	T4553	punct	-,T50
R3068	T4555	T4547	punct	", ",observed
R3069	T4556	T4547	prep	on,observed
R3070	T4557	T4558	det	the,hand
R3071	T4558	T4556	pobj	hand,on
R3072	T4559	T4558	amod	other,hand
R3073	T4560	T4547	punct	", ",observed
R3074	T4561	T4562	amod	significant,displacement
R3075	T4562	T4547	nsubjpass	displacement,observed
R3076	T4563	T4547	auxpass	was,observed
R3077	T4564	T4547	punct	", ",observed
R3078	T4565	T4547	advcl	indicating,observed
R3079	T4566	T4567	mark	that,required
R3080	T4567	T4565	ccomp	required,indicating
R3081	T4568	T4569	det	a,segment
R3082	T4569	T4567	nsubjpass	segment,required
R3083	T4570	T4571	amod	single,stranded
R3084	T4571	T4569	amod	stranded,segment
R3085	T4572	T4571	punct	-,stranded
R3086	T4573	T4569	prep	of,segment
R3087	T4574	T4575	advmod	at,least
R3088	T4575	T4576	advmod	least,40
R3089	T4576	T4577	nummod	40,nt
R3090	T4577	T4573	pobj	nt,of
R3091	T4578	T4577	amod	long,nt
R3092	T4579	T4567	auxpass	is,required
R3093	T4580	T4567	prep	for,required
R3094	T4581	T4582	amod	efficient,actions
R3095	T4582	T4580	pobj	actions,for
R3096	T4583	T4582	compound	helicase,actions
R3097	T4584	T4582	prep	on,actions
R3098	T4585	T4586	compound	bubble,substrates
R3099	T4586	T4584	pobj	substrates,on
R3100	T4587	T4588	punct	(,2B
R3101	T4588	T4567	parataxis	2B,required
R3102	T4589	T4588	compound	Figure,2B
R3103	T4590	T4588	punct	),2B
R3104	T4591	T4547	punct	.,observed
R3105	T4593	T4594	advcl	Taken,are
R3106	T4595	T4593	advmod	together,Taken
R3107	T4596	T4594	punct	", ",are
R3108	T4597	T4598	det	the,results
R3109	T4598	T4594	nsubj	results,are
R3110	T4599	T4598	amod	above,results
R3111	T4600	T4594	acomp	consistent,are
R3112	T4601	T4600	prep	with,consistent
R3113	T4602	T4603	det	the,notion
R3114	T4603	T4601	pobj	notion,with
R3115	T4604	T4605	mark	that,unwinds
R3116	T4605	T4603	acl	unwinds,notion
R3117	T4606	T4607	det	the,complex
R3118	T4607	T4605	nsubj	complex,unwinds
R3119	T4608	T4607	nmod	Mcm4,complex
R3120	T4609	T4608	punct	/,Mcm4
R3121	T4610	T4608	nummod	6,Mcm4
R3122	T4611	T4608	punct	/,Mcm4
R3123	T4612	T4608	nummod	7,Mcm4
R3124	T4613	T4607	punct	", ",complex
R3125	T4614	T4607	acl	loaded,complex
R3126	T4615	T4614	prep	onto,loaded
R3127	T4616	T4615	pobj	DNA,onto
R3128	T4617	T4614	prep	as,loaded
R3129	T4618	T4619	det	a,complex
R3130	T4619	T4617	pobj	complex,as
R3131	T4620	T4621	amod	double,hexameric
R3132	T4621	T4619	amod	hexameric,complex
R3133	T4622	T4605	punct	", ",unwinds
R3134	T4623	T4605	advmod	efficiently,unwinds
R3135	T4624	T4625	det	the,region
R3136	T4625	T4605	dobj	region,unwinds
R3137	T4626	T4625	compound	duplex,region
R3138	T4627	T4625	prep	of,region
R3139	T4628	T4629	det	the,DNA
R3140	T4629	T4627	pobj	DNA,of
R3141	T4630	T4629	compound	substrate,DNA
R3142	T4631	T4594	punct	.,are
R3145	T4737	T4738	det	Both,strands
R3146	T4738	T4739	nsubjpass	strands,required
R3147	T4740	T4738	prep	of,strands
R3148	T4741	T4742	npadvmod	thymine,rich
R3149	T4742	T4744	amod	rich,segments
R3150	T4743	T4742	punct	-,rich
R3151	T4744	T4740	pobj	segments,of
R3152	T4745	T4746	amod	single,stranded
R3153	T4746	T4744	amod	stranded,segments
R3154	T4747	T4746	punct	-,stranded
R3155	T4748	T4739	auxpass	are,required
R3156	T4749	T4739	prep	for,required
R3157	T4750	T4751	amod	efficient,unwinding
R3158	T4751	T4749	pobj	unwinding,for
R3159	T4752	T4751	prep	of,unwinding
R3160	T4753	T4754	compound	bubble,DNA
R3161	T4754	T4752	pobj	DNA,of
R3162	T4755	T4751	prep	by,unwinding
R3163	T4756	T4755	pobj	Mcm4,by
R3164	T4757	T4756	punct	/,Mcm4
R3165	T4758	T4756	nummod	6,Mcm4
R3166	T4759	T4756	punct	/,Mcm4
R3167	T4760	T4756	nummod	7,Mcm4
R3168	T4762	T4763	amod	Symmetric,binding
R3169	T4763	T4764	nsubj	binding,suggests
R3170	T4765	T4763	prep	of,binding
R3171	T4766	T4767	det	the,hexamer
R3172	T4767	T4765	pobj	hexamer,of
R3173	T4768	T4767	nmod	Mcm4,hexamer
R3174	T4769	T4768	punct	/,Mcm4
R3175	T4770	T4768	nummod	6,Mcm4
R3176	T4771	T4768	punct	/,Mcm4
R3177	T4772	T4768	nummod	7,Mcm4
R3178	T4773	T4767	amod	double,hexamer
R3179	T4774	T4763	prep	to,binding
R3180	T4775	T4774	pobj	bubble,to
R3181	T4776	T4777	mark	that,catalyzes
R3182	T4777	T4764	ccomp	catalyzes,suggests
R3183	T4778	T4779	det	each,hexamer
R3184	T4779	T4777	nsubj	hexamer,catalyzes
R3185	T4780	T4777	dobj	unwinding,catalyzes
R3186	T4781	T4780	prep	of,unwinding
R3187	T4782	T4781	pobj	one,of
R3188	T4783	T4782	prep	of,one
R3189	T4784	T4785	det	the,segments
R3190	T4785	T4783	pobj	segments,of
R3191	T4786	T4785	nummod	two,segments
R3192	T4787	T4785	compound	duplex,segments
R3193	T4788	T4764	punct	.,suggests
R3194	T4790	T4791	nsubj	We,asked
R3195	T4792	T4793	mark	whether,required
R3196	T4793	T4791	ccomp	required,asked
R3197	T4794	T4793	nsubjpass	activation,required
R3198	T4795	T4794	prep	of,activation
R3199	T4796	T4797	det	both,hexamers
R3200	T4797	T4795	pobj	hexamers,of
R3201	T4798	T4793	auxpass	is,required
R3202	T4799	T4793	prep	for,required
R3203	T4800	T4799	pobj	unwinding,for
R3204	T4801	T4800	prep	of,unwinding
R3205	T4802	T4803	det	the,substrate
R3206	T4803	T4801	pobj	substrate,of
R3207	T4804	T4803	compound	bubble,substrate
R3208	T4805	T4791	punct	.,asked
R3209	T4807	T4808	det	The,substrate
R3210	T4808	T4811	nsubj	substrate,contains
R3211	T4809	T4808	amod	preferred,substrate
R3212	T4810	T4808	compound	bubble,substrate
R3213	T4812	T4813	dep	which,unwound
R3214	T4813	T4808	relcl	unwound,substrate
R3215	T4814	T4813	auxpass	is,unwound
R3216	T4815	T4813	advmod	efficiently,unwound
R3217	T4816	T4813	agent	by,unwound
R3218	T4817	T4816	pobj	Mcm4,by
R3219	T4818	T4817	punct	/,Mcm4
R3220	T4819	T4817	nummod	6,Mcm4
R3221	T4820	T4817	punct	/,Mcm4
R3222	T4821	T4817	nummod	7,Mcm4
R3223	T4822	T4823	compound	thymine,stretches
R3224	T4823	T4811	dobj	stretches,contains
R3225	T4824	T4823	prep	on,stretches
R3226	T4825	T4826	det	both,strands
R3227	T4826	T4824	pobj	strands,on
R3228	T4827	T4826	prep	of,strands
R3229	T4828	T4829	det	the,segment
R3230	T4829	T4827	pobj	segment,of
R3231	T4830	T4831	amod	single,stranded
R3232	T4831	T4829	amod	stranded,segment
R3233	T4832	T4831	punct	-,stranded
R3234	T4833	T4811	punct	.,contains
R3235	T4835	T4836	nsubj	We,constructed
R3236	T4837	T4838	det	a,hetero-bubble
R3237	T4838	T4836	dobj	hetero-bubble,constructed
R3238	T4839	T4838	punct	(,hetero-bubble
R3239	T4840	T4841	compound	Bub66,G
R3240	T4841	T4838	appos	G,hetero-bubble
R3241	T4842	T4841	punct	/,G
R3242	T4843	T4841	compound	T,G
R3243	T4844	T4841	punct	–,G
R3244	T4845	T4841	punct	-,G
R3245	T4846	T4841	amod	rich,G
R3246	T4847	T4838	punct	),hetero-bubble
R3247	T4848	T4849	prep	in,is
R3248	T4849	T4838	relcl	is,hetero-bubble
R3249	T4850	T4848	pobj	which,in
R3250	T4851	T4852	nummod	one,strand
R3251	T4852	T4849	nsubj	strand,is
R3252	T4853	T4854	npadvmod	T,rich
R3253	T4854	T4849	acomp	rich,is
R3254	T4855	T4854	punct	-,rich
R3255	T4856	T4857	punct	(,same
R3256	T4857	T4854	parataxis	same,rich
R3257	T4858	T4857	prep	as,same
R3258	T4859	T4860	compound	Bub66,T
R3259	T4860	T4858	pobj	T,as
R3260	T4861	T4860	punct	/,T
R3261	T4862	T4860	punct	-,T
R3262	T4863	T4860	amod	rich,T
R3263	T4864	T4857	punct	),same
R3264	T4865	T4849	cc	and,is
R3265	T4866	T4867	det	the,strand
R3266	T4867	T4869	nsubj	strand,is
R3267	T4868	T4867	amod	other,strand
R3268	T4869	T4849	conj	is,is
R3269	T4870	T4871	npadvmod	G,rich
R3270	T4871	T4869	acomp	rich,is
R3271	T4872	T4871	punct	-,rich
R3272	T4873	T4874	punct	(,same
R3273	T4874	T4871	parataxis	same,rich
R3274	T4875	T4874	prep	as,same
R3275	T4876	T4877	compound	Bub66,G
R3276	T4877	T4875	pobj	G,as
R3277	T4878	T4877	punct	/,G
R3278	T4879	T4877	punct	-,G
R3279	T4880	T4877	amod	rich,G
R3280	T4881	T4874	punct	),same
R3281	T4882	T4883	punct	(,13
R3282	T4883	T4871	parataxis	13,rich
R3283	T4884	T4883	punct	),13
R3284	T4885	T4836	punct	", ",constructed
R3285	T4886	T4836	cc	and,constructed
R3286	T4887	T4888	compound	helicase,assays
R3287	T4888	T4889	nsubjpass	assays,conducted
R3288	T4889	T4836	conj	conducted,constructed
R3289	T4890	T4889	auxpass	were,conducted
R3290	T4891	T4889	prep	with,conducted
R3291	T4892	T4893	amod	increasing,amount
R3292	T4893	T4891	pobj	amount,with
R3293	T4894	T4893	prep	of,amount
R3294	T4895	T4894	pobj	Mcm4,of
R3295	T4896	T4895	punct	/,Mcm4
R3296	T4897	T4895	nummod	6,Mcm4
R3297	T4898	T4895	punct	/,Mcm4
R3298	T4899	T4895	nummod	7,Mcm4
R3299	T4900	T4889	punct	.,conducted
R3300	T4902	T4903	advmod	Only,unwinding
R3301	T4903	T4905	nsubjpass	unwinding,detected
R3302	T4904	T4903	amod	weak,unwinding
R3303	T4906	T4905	auxpass	was,detected
R3304	T4907	T4905	prep	on,detected
R3305	T4908	T4909	det	this,substrate
R3306	T4909	T4907	pobj	substrate,on
R3307	T4910	T4905	prep	compared,detected
R3308	T4911	T4910	prep	to,compared
R3309	T4912	T4913	compound	Bub66,T
R3310	T4913	T4911	pobj	T,to
R3311	T4914	T4913	punct	/,T
R3312	T4915	T4913	punct	-,T
R3313	T4916	T4913	amod	rich,T
R3314	T4917	T4918	punct	(,3A
R3315	T4918	T4905	parataxis	3A,detected
R3316	T4919	T4918	compound	Figure,3A
R3317	T4920	T4918	punct	),3A
R3318	T4921	T4905	punct	.,detected
R3319	T4923	T4924	mark	Since,bind
R3320	T4924	T4931	advcl	bind,is
R3321	T4925	T4924	nsubj	Mcm4,bind
R3322	T4926	T4925	punct	/,Mcm4
R3323	T4927	T4925	nummod	6,Mcm4
R3324	T4928	T4925	punct	/,Mcm4
R3325	T4929	T4925	nummod	7,Mcm4
R3326	T4930	T4924	aux	can,bind
R3327	T4932	T4924	prep	to,bind
R3328	T4933	T4934	compound	Bub66,G
R3329	T4934	T4932	pobj	G,to
R3330	T4935	T4934	punct	/,G
R3331	T4936	T4934	compound	T,G
R3332	T4937	T4934	punct	–,G
R3333	T4938	T4934	punct	-,G
R3334	T4939	T4934	amod	rich,G
R3335	T4940	T4924	cc	and,bind
R3336	T4941	T4924	conj	forms,bind
R3337	T4942	T4943	det	a,complex
R3338	T4943	T4941	dobj	complex,forms
R3339	T4944	T4945	amod	double,hexameric
R3340	T4945	T4943	amod	hexameric,complex
R3341	T4946	T4947	punct	(,shown
R3342	T4947	T4941	parataxis	shown,forms
R3343	T4948	T4947	nsubj	data,shown
R3344	T4949	T4947	neg	not,shown
R3345	T4950	T4947	punct	),shown
R3346	T4951	T4931	punct	", ",is
R3347	T4952	T4953	det	the,unwinding
R3348	T4953	T4931	nsubj	unwinding,is
R3349	T4954	T4953	amod	defective,unwinding
R3350	T4955	T4931	neg	not,is
R3351	T4956	T4931	prep	due,is
R3352	T4957	T4956	pcomp	to,due
R3353	T4958	T4959	amod	inefficient,loading
R3354	T4959	T4956	pobj	loading,due
R3355	T4960	T4959	prep	of,loading
R3356	T4961	T4962	det	the,helicase
R3357	T4962	T4960	pobj	helicase,of
R3358	T4963	T4959	prep	onto,loading
R3359	T4964	T4965	det	the,substrate
R3360	T4965	T4963	pobj	substrate,onto
R3361	T4966	T4931	punct	.,is
R3362	T4968	T4969	mark	If,dissociates
R3363	T4969	T4979	advcl	dissociates,result
R3364	T4970	T4971	nummod	one,hexamer
R3365	T4971	T4969	nsubj	hexamer,dissociates
R3366	T4972	T4971	acl	interacting,hexamer
R3367	T4973	T4972	prep	with,interacting
R3368	T4974	T4975	det	the,strand
R3369	T4975	T4973	pobj	strand,with
R3370	T4976	T4977	npadvmod	thymine,rich
R3371	T4977	T4975	amod	rich,strand
R3372	T4978	T4977	punct	-,rich
R3373	T4980	T4969	prep	from,dissociates
R3374	T4981	T4982	det	the,hexamer
R3375	T4982	T4980	pobj	hexamer,from
R3376	T4983	T4982	amod	other,hexamer
R3377	T4984	T4969	cc	and,dissociates
R3378	T4985	T4969	conj	displaces,dissociates
R3379	T4986	T4987	advmod	only,one
R3380	T4987	T4985	dobj	one,displaces
R3381	T4988	T4987	prep	of,one
R3382	T4989	T4990	det	the,segments
R3383	T4990	T4988	pobj	segments,of
R3384	T4991	T4990	compound	duplex,segments
R3385	T4992	T4979	punct	", ",result
R3386	T4993	T4979	advmod	then,result
R3387	T4994	T4979	nsubj	it,result
R3388	T4995	T4979	aux	would,result
R3389	T4996	T4979	prep	in,result
R3390	T4997	T4996	pobj	generation,in
R3391	T4998	T4997	prep	of,generation
R3392	T4999	T5000	compound	Y,fork
R3393	T5000	T5002	compound	fork,structures
R3394	T5001	T5000	punct	-,fork
R3395	T5002	T4998	pobj	structures,of
R3396	T5003	T5004	dep	which,displaced
R3397	T5004	T5002	relcl	displaced,structures
R3398	T5005	T5004	aux	could,displaced
R3399	T5006	T5004	neg	not,displaced
R3400	T5007	T5004	auxpass	be,displaced
R3401	T5008	T5004	advmod	further,displaced
R3402	T5009	T5004	prep	due,displaced
R3403	T5010	T5009	pcomp	to,due
R3404	T5011	T5012	det	the,presence
R3405	T5012	T5009	pobj	presence,due
R3406	T5013	T5012	prep	of,presence
R3407	T5014	T5015	npadvmod	G,rich
R3408	T5015	T5017	amod	rich,tail
R3409	T5016	T5015	punct	-,rich
R3410	T5017	T5013	pobj	tail,of
R3411	T5018	T5017	nummod	3,tail
R3412	T5019	T5018	punct	′,3
R3413	T5020	T5017	punct	-,tail
R3414	T5021	T5004	cc	and,displaced
R3415	T5022	T5023	aux	would,appear
R3416	T5023	T5004	conj	appear,displaced
R3417	T5024	T5023	prep	on,appear
R3418	T5025	T5026	det	the,gel
R3419	T5026	T5024	pobj	gel,on
R3420	T5027	T4979	punct	.,result
R3421	T5029	T5030	advmod	However,detect
R3422	T5031	T5030	punct	", ",detect
R3423	T5032	T5030	nsubj	we,detect
R3424	T5033	T5030	aux	did,detect
R3425	T5034	T5030	neg	not,detect
R3426	T5035	T5036	amod	such,intermediates
R3427	T5036	T5030	dobj	intermediates,detect
R3428	T5037	T5030	prep	during,detect
R3429	T5038	T5039	det	the,course
R3430	T5039	T5037	pobj	course,during
R3431	T5040	T5039	prep	of,course
R3432	T5041	T5042	det	the,experiment
R3433	T5042	T5040	pobj	experiment,of
R3434	T5043	T5044	punct	(,3B
R3435	T5044	T5030	parataxis	3B,detect
R3436	T5045	T5044	compound	Figure,3B
R3437	T5046	T5044	punct	),3B
R3438	T5047	T5030	punct	", ",detect
R3439	T5048	T5030	advcl	suggesting,detect
R3440	T5049	T5050	mark	that,displaced
R3441	T5050	T5048	ccomp	displaced,suggesting
R3442	T5051	T5050	nsubjpass	bubble,displaced
R3443	T5052	T5050	aux	can,displaced
R3444	T5053	T5050	auxpass	be,displaced
R3445	T5054	T5050	advmod	efficiently,displaced
R3446	T5055	T5056	advmod	only,activated
R3447	T5056	T5050	advcl	activated,displaced
R3448	T5057	T5056	advmod	when,activated
R3449	T5058	T5059	nummod	two,molecules
R3450	T5059	T5056	nsubjpass	molecules,activated
R3451	T5060	T5059	prep	of,molecules
R3452	T5061	T5062	det	the,hexamers
R3453	T5062	T5060	pobj	hexamers,of
R3454	T5063	T5062	nmod	Mcm4,hexamers
R3455	T5064	T5063	punct	/,Mcm4
R3456	T5065	T5063	nummod	6,Mcm4
R3457	T5066	T5063	punct	/,Mcm4
R3458	T5067	T5063	nummod	7,Mcm4
R3459	T5068	T5056	auxpass	are,activated
R3460	T5069	T5056	advmod	simultaneously,activated
R3461	T5070	T5030	punct	.,detect
R3464	T5260	T5261	nmod	Binding,actions
R3465	T5262	T5260	cc	and,Binding
R3466	T5263	T5260	conj	helicase,Binding
R3467	T5264	T5261	prep	of,actions
R3468	T5265	T5264	pobj	Mcm4,of
R3469	T5266	T5265	punct	/,Mcm4
R3470	T5267	T5265	nummod	6,Mcm4
R3471	T5268	T5265	punct	/,Mcm4
R3472	T5269	T5265	nummod	7,Mcm4
R3473	T5270	T5261	prep	on,actions
R3474	T5271	T5272	amod	forked,substrates
R3475	T5272	T5270	pobj	substrates,on
R3476	T5273	T5271	cc	and,forked
R3477	T5274	T5275	nummod	3,extension
R3478	T5275	T5271	conj	extension,forked
R3479	T5276	T5274	punct	′,3
R3480	T5277	T5274	punct	-,3
R3481	T5278	T5274	cc	or,3
R3482	T5279	T5274	conj	5,3
R3483	T5280	T5279	punct	′,5
R3484	T5281	T5275	punct	-,extension
R3485	T5283	T5284	prep	In,constructed
R3486	T5285	T5283	pobj	order,In
R3487	T5286	T5287	aux	to,examine
R3488	T5287	T5285	acl	examine,order
R3489	T5288	T5289	advmod	more,precisely
R3490	T5289	T5287	advmod	precisely,examine
R3491	T5290	T5291	det	the,interaction
R3492	T5291	T5287	dobj	interaction,examine
R3493	T5292	T5291	cc	and,interaction
R3494	T5293	T5294	compound	helicase,actions
R3495	T5294	T5291	conj	actions,interaction
R3496	T5295	T5291	prep	with,interaction
R3497	T5296	T5297	amod	forked,substrates
R3498	T5297	T5295	pobj	substrates,with
R3499	T5298	T5284	punct	", ",constructed
R3500	T5299	T5300	compound	Y,fork
R3501	T5300	T5302	compound	fork,substrates
R3502	T5301	T5300	punct	-,fork
R3503	T5302	T5284	nsubjpass	substrates,constructed
R3504	T5303	T5302	acl	containing,substrates
R3505	T5304	T5305	det	a,strand
R3506	T5305	T5303	dobj	strand,containing
R3507	T5306	T5305	amod	nascent,strand
R3508	T5307	T5305	amod	leading,strand
R3509	T5308	T5305	punct	(,strand
R3510	T5309	T5310	compound	A,fork
R3511	T5310	T5305	appos	fork,strand
R3512	T5311	T5310	punct	-,fork
R3513	T5312	T5310	punct	[,fork
R3514	T5313	T5310	nummod	3,fork
R3515	T5314	T5310	punct	′,fork
R3516	T5315	T5305	punct	],strand
R3517	T5316	T5305	punct	),strand
R3518	T5317	T5305	punct	", ",strand
R3519	T5318	T5319	amod	nascent,strand
R3520	T5319	T5305	appos	strand,strand
R3521	T5320	T5319	amod	lagging,strand
R3522	T5321	T5319	punct	(,strand
R3523	T5322	T5323	compound	A,fork
R3524	T5323	T5319	appos	fork,strand
R3525	T5324	T5323	punct	-,fork
R3526	T5325	T5323	punct	[,fork
R3527	T5326	T5323	nummod	5,fork
R3528	T5327	T5323	punct	′,fork
R3529	T5328	T5305	punct	],strand
R3530	T5329	T5305	punct	),strand
R3531	T5330	T5305	punct	", ",strand
R3532	T5331	T5305	cc	or,strand
R3533	T5332	T5305	conj	both,strand
R3534	T5333	T5332	prep	of,both
R3535	T5334	T5333	pobj	them,of
R3536	T5335	T5332	punct	(,both
R3537	T5336	T5337	nmod	A,fork
R3538	T5337	T5339	nmod	fork,structures
R3539	T5338	T5337	punct	-,fork
R3540	T5339	T5332	appos	structures,both
R3541	T5340	T5337	punct	[,fork
R3542	T5341	T5337	appos	3,fork
R3543	T5342	T5341	punct	′,3
R3544	T5343	T5341	punct	",",3
R3545	T5344	T5341	appos	5,3
R3546	T5345	T5344	punct	′,5
R3547	T5346	T5337	punct	],fork
R3548	T5347	T5337	punct	),fork
R3549	T5348	T5337	punct	", ",fork
R3550	T5349	T5350	advmod	as,as
R3551	T5350	T5337	cc	as,fork
R3552	T5351	T5350	advmod	well,as
R3553	T5352	T5353	nummod	5,extension
R3554	T5353	T5337	conj	extension,fork
R3555	T5354	T5352	punct	′,5
R3556	T5355	T5353	punct	-,extension
R3557	T5356	T5353	cc	or,extension
R3558	T5357	T5358	nummod	3,extension
R3559	T5358	T5353	conj	extension,extension
R3560	T5359	T5357	punct	′,3
R3561	T5360	T5358	punct	-,extension
R3562	T5361	T5284	auxpass	were,constructed
R3563	T5362	T5284	punct	.,constructed
R3564	T5364	T5365	nsubj	Mcm4,bound
R3565	T5366	T5364	punct	/,Mcm4
R3566	T5367	T5364	nummod	6,Mcm4
R3567	T5368	T5364	punct	/,Mcm4
R3568	T5369	T5364	nummod	7,Mcm4
R3569	T5370	T5365	prep	to,bound
R3570	T5371	T5372	compound	A,fork
R3571	T5372	T5370	pobj	fork,to
R3572	T5373	T5372	punct	-,fork
R3573	T5374	T5372	punct	[,fork
R3574	T5375	T5372	nummod	3,fork
R3575	T5376	T5372	punct	′,fork
R3576	T5377	T5372	punct	],fork
R3577	T5378	T5372	cc	or,fork
R3578	T5379	T5380	compound	A,fork
R3579	T5380	T5372	conj	fork,fork
R3580	T5381	T5380	punct	-,fork
R3581	T5382	T5380	punct	[,fork
R3582	T5383	T5380	nummod	5,fork
R3583	T5384	T5380	punct	′,fork
R3584	T5385	T5365	punct	],bound
R3585	T5386	T5365	prep	with,bound
R3586	T5387	T5386	pobj	affinity,with
R3587	T5388	T5387	amod	similar,affinity
R3588	T5389	T5388	prep	to,similar
R3589	T5390	T5389	pobj	that,to
R3590	T5391	T5390	prep	of,that
R3591	T5392	T5393	det	a,fork
R3592	T5393	T5391	pobj	fork,of
R3593	T5394	T5393	amod	simple,fork
R3594	T5395	T5393	compound	Y,fork
R3595	T5396	T5393	punct	-,fork
R3596	T5397	T5398	punct	(,4A
R3597	T5398	T5365	parataxis	4A,bound
R3598	T5399	T5398	compound	Figure,4A
R3599	T5400	T5398	cc	and,4A
R3600	T5401	T5402	nsubj	data,shown
R3601	T5402	T5398	conj	shown,4A
R3602	T5403	T5402	neg	not,shown
R3603	T5404	T5398	punct	),4A
R3604	T5405	T5365	punct	.,bound
R3605	T5407	T5408	prep	In,bound
R3606	T5409	T5407	pobj	contrast,In
R3607	T5410	T5408	punct	", ",bound
R3608	T5411	T5412	compound	PriA,helicase
R3609	T5412	T5408	nsubj	helicase,bound
R3610	T5413	T5412	punct	", ",helicase
R3611	T5414	T5412	acl	used,helicase
R3612	T5415	T5414	prep	as,used
R3613	T5416	T5417	det	a,control
R3614	T5417	T5415	pobj	control,as
R3615	T5418	T5408	punct	", ",bound
R3616	T5419	T5408	prep	to,bound
R3617	T5420	T5421	compound	A,fork
R3618	T5421	T5419	pobj	fork,to
R3619	T5422	T5421	punct	-,fork
R3620	T5423	T5421	punct	[,fork
R3621	T5424	T5421	nummod	3,fork
R3622	T5425	T5421	punct	′,fork
R3623	T5426	T5408	punct	],bound
R3624	T5427	T5428	advmod	more,efficiently
R3625	T5428	T5408	advmod	efficiently,bound
R3626	T5429	T5428	prep	than,efficiently
R3627	T5430	T5429	prep	to,than
R3628	T5431	T5432	compound	A,fork
R3629	T5432	T5430	pobj	fork,to
R3630	T5433	T5432	punct	-,fork
R3631	T5434	T5432	punct	[,fork
R3632	T5435	T5432	nummod	5,fork
R3633	T5436	T5432	punct	′,fork
R3634	T5437	T5408	punct	],bound
R3635	T5438	T5408	punct	", ",bound
R3636	T5439	T5440	mark	as,reported
R3637	T5440	T5408	advcl	reported,bound
R3638	T5441	T5440	advmod	previously,reported
R3639	T5442	T5443	punct	(,25
R3640	T5443	T5440	parataxis	25,reported
R3641	T5444	T5443	nummod	23,25
R3642	T5445	T5443	punct	",",25
R3643	T5446	T5443	punct	),25
R3644	T5447	T5408	punct	.,bound
R3645	T5449	T5450	nsubj	Mcm4,bound
R3646	T5451	T5449	punct	/,Mcm4
R3647	T5452	T5449	nummod	6,Mcm4
R3648	T5453	T5449	punct	/,Mcm4
R3649	T5454	T5449	nummod	7,Mcm4
R3650	T5455	T5450	advmod	also,bound
R3651	T5456	T5450	prep	to,bound
R3652	T5457	T5458	nummod	5,extension
R3653	T5458	T5456	pobj	extension,to
R3654	T5459	T5457	punct	′,5
R3655	T5460	T5458	punct	-,extension
R3656	T5461	T5458	cc	and,extension
R3657	T5462	T5463	nummod	3,extension
R3658	T5463	T5458	conj	extension,extension
R3659	T5464	T5462	punct	′,3
R3660	T5465	T5463	punct	-,extension
R3661	T5466	T5450	punct	", ",bound
R3662	T5467	T5468	dep	albeit,with
R3663	T5468	T5450	prep	with,bound
R3664	T5469	T5470	advmod	slightly,reduced
R3665	T5470	T5471	amod	reduced,affinity
R3666	T5471	T5468	pobj	affinity,with
R3667	T5472	T5471	prep	compared,affinity
R3668	T5473	T5472	prep	to,compared
R3669	T5474	T5475	compound	A,fork
R3670	T5475	T5473	pobj	fork,to
R3671	T5476	T5475	punct	-,fork
R3672	T5477	T5475	punct	[,fork
R3673	T5478	T5475	nummod	3,fork
R3674	T5479	T5475	punct	′,fork
R3675	T5480	T5475	punct	],fork
R3676	T5481	T5475	cc	and,fork
R3677	T5482	T5483	compound	A,fork
R3678	T5483	T5475	conj	fork,fork
R3679	T5484	T5483	punct	-,fork
R3680	T5485	T5483	punct	[,fork
R3681	T5486	T5483	nummod	5,fork
R3682	T5487	T5483	punct	′,fork
R3683	T5488	T5450	punct	],bound
R3684	T5489	T5490	punct	(,4A
R3685	T5490	T5450	parataxis	4A,bound
R3686	T5491	T5490	compound	Figure,4A
R3687	T5492	T5490	punct	),4A
R3688	T5493	T5450	punct	.,bound
R3689	T5495	T5496	nsubj	Mcm4,bound
R3690	T5497	T5495	punct	/,Mcm4
R3691	T5498	T5495	nummod	6,Mcm4
R3692	T5499	T5495	punct	/,Mcm4
R3693	T5500	T5495	nummod	7,Mcm4
R3694	T5501	T5496	prep	to,bound
R3695	T5502	T5503	compound	A,fork
R3696	T5503	T5501	pobj	fork,to
R3697	T5504	T5503	punct	-,fork
R3698	T5505	T5503	punct	[,fork
R3699	T5506	T5503	appos	3,fork
R3700	T5507	T5506	punct	′,3
R3701	T5508	T5506	punct	",",3
R3702	T5509	T5506	appos	5,3
R3703	T5510	T5509	punct	′,5
R3704	T5511	T5496	punct	],bound
R3705	T5512	T5496	prep	with,bound
R3706	T5513	T5514	advmod	much,lower
R3707	T5514	T5515	amod	lower,affinity
R3708	T5515	T5512	pobj	affinity,with
R3709	T5516	T5496	punct	", ",bound
R3710	T5517	T5496	cc	and,bound
R3711	T5518	T5519	det	the,complex
R3712	T5519	T5520	nsubj	complex,gave
R3713	T5520	T5496	conj	gave,bound
R3714	T5521	T5520	dobj	rise,gave
R3715	T5522	T5520	prep	to,gave
R3716	T5523	T5524	det	a,band
R3717	T5524	T5522	pobj	band,to
R3718	T5525	T5524	amod	single,band
R3719	T5526	T5520	punct	.,gave
R3720	T5528	T5529	det	These,findings
R3721	T5529	T5530	nsubj	findings,indicate
R3722	T5531	T5532	mark	that,loaded
R3723	T5532	T5530	ccomp	loaded,indicate
R3724	T5533	T5534	det	the,complex
R3725	T5534	T5532	nsubjpass	complex,loaded
R3726	T5535	T5534	nmod	Mcm4,complex
R3727	T5536	T5535	punct	/,Mcm4
R3728	T5537	T5535	nummod	6,Mcm4
R3729	T5538	T5535	punct	/,Mcm4
R3730	T5539	T5535	nummod	7,Mcm4
R3731	T5540	T5532	auxpass	is,loaded
R3732	T5541	T5532	prep	onto,loaded
R3733	T5542	T5541	pobj	DNA,onto
R3734	T5543	T5532	advmod	preferentially,loaded
R3735	T5544	T5543	cc	and,preferentially
R3736	T5545	T5543	conj	efficiently,preferentially
R3737	T5546	T5532	prep	through,loaded
R3738	T5547	T5548	amod	single,stranded
R3739	T5548	T5550	amod	stranded,region
R3740	T5549	T5548	punct	-,stranded
R3741	T5550	T5546	pobj	region,through
R3742	T5551	T5532	punct	", ",loaded
R3743	T5552	T5532	advmod	regardless,loaded
R3744	T5553	T5552	prep	of,regardless
R3745	T5554	T5555	det	the,presence
R3746	T5555	T5553	pobj	presence,of
R3747	T5556	T5555	prep	of,presence
R3748	T5557	T5558	nummod	3,terminus
R3749	T5558	T5556	pobj	terminus,of
R3750	T5559	T5557	punct	′,3
R3751	T5560	T5557	punct	-,3
R3752	T5561	T5557	cc	or,3
R3753	T5562	T5557	conj	5,3
R3754	T5563	T5562	punct	′,5
R3755	T5564	T5558	punct	-,terminus
R3756	T5565	T5530	punct	.,indicate
R3757	T5567	T5568	prep	In,unwound
R3758	T5569	T5567	pobj	contrast,In
R3759	T5570	T5568	punct	", ",unwound
R3760	T5571	T5572	advmod	only,substrate
R3761	T5572	T5568	nsubjpass	substrate,unwound
R3762	T5573	T5572	det	the,substrate
R3763	T5574	T5572	acl	containing,substrate
R3764	T5575	T5576	det	a,tail
R3765	T5576	T5574	dobj	tail,containing
R3766	T5577	T5576	nummod	3,tail
R3767	T5578	T5577	punct	′,3
R3768	T5579	T5580	amod	single,stranded
R3769	T5580	T5576	amod	stranded,tail
R3770	T5581	T5580	punct	-,stranded
R3771	T5582	T5576	compound	DNA,tail
R3772	T5583	T5584	punct	(,fork
R3773	T5584	T5572	appos	fork,substrate
R3774	T5585	T5584	compound	A,fork
R3775	T5586	T5584	punct	-,fork
R3776	T5587	T5584	punct	[,fork
R3777	T5588	T5584	nummod	5,fork
R3778	T5589	T5584	punct	′,fork
R3779	T5590	T5568	punct	],unwound
R3780	T5591	T5568	punct	),unwound
R3781	T5592	T5568	auxpass	was,unwound
R3782	T5593	T5568	advmod	efficiently,unwound
R3783	T5594	T5568	cc	and,unwound
R3784	T5595	T5596	compound	A,fork
R3785	T5596	T5598	nsubjpass	fork,unwound
R3786	T5597	T5596	punct	-,fork
R3787	T5598	T5568	conj	unwound,unwound
R3788	T5599	T5596	punct	[,fork
R3789	T5600	T5596	nummod	3,fork
R3790	T5601	T5596	punct	′,fork
R3791	T5602	T5596	punct	],fork
R3792	T5603	T5596	cc	or,fork
R3793	T5604	T5605	compound	A,fork
R3794	T5605	T5596	conj	fork,fork
R3795	T5606	T5605	punct	-,fork
R3796	T5607	T5605	punct	[,fork
R3797	T5608	T5605	appos	3,fork
R3798	T5609	T5608	punct	′,3
R3799	T5610	T5608	punct	",",3
R3800	T5611	T5608	appos	5,3
R3801	T5612	T5611	punct	′,5
R3802	T5613	T5598	punct	],unwound
R3803	T5614	T5598	punct	", ",unwound
R3804	T5615	T5598	auxpass	was,unwound
R3805	T5616	T5598	neg	not,unwound
R3806	T5617	T5598	agent	by,unwound
R3807	T5618	T5617	pobj	Mcm4,by
R3808	T5619	T5618	punct	/,Mcm4
R3809	T5620	T5618	nummod	6,Mcm4
R3810	T5621	T5618	punct	/,Mcm4
R3811	T5622	T5618	nummod	7,Mcm4
R3812	T5623	T5624	punct	(,4B
R3813	T5624	T5598	parataxis	4B,unwound
R3814	T5625	T5624	compound	Figure,4B
R3815	T5626	T5624	punct	),4B
R3816	T5627	T5568	punct	.,unwound
R3817	T5629	T5630	nsubjpass	Absence,expected
R3818	T5631	T5629	prep	of,Absence
R3819	T5632	T5633	compound	helicase,actions
R3820	T5633	T5631	pobj	actions,of
R3821	T5634	T5629	prep	on,Absence
R3822	T5635	T5636	compound	A,fork
R3823	T5636	T5634	pobj	fork,on
R3824	T5637	T5636	punct	-,fork
R3825	T5638	T5636	punct	[,fork
R3826	T5639	T5636	appos	3,fork
R3827	T5640	T5639	punct	′,3
R3828	T5641	T5639	punct	",",3
R3829	T5642	T5639	appos	5,3
R3830	T5643	T5642	punct	′,5
R3831	T5644	T5636	punct	],fork
R3832	T5645	T5636	acl	composed,fork
R3833	T5646	T5647	advmod	only,of
R3834	T5647	T5645	prep	of,composed
R3835	T5648	T5649	compound	duplex,regions
R3836	T5649	T5647	pobj	regions,of
R3837	T5650	T5630	auxpass	is,expected
R3838	T5651	T5652	mark	since,is
R3839	T5652	T5630	advcl	is,expected
R3840	T5653	T5654	amod	double,stranded
R3841	T5654	T5656	amod	stranded,DNA
R3842	T5655	T5654	punct	-,stranded
R3843	T5656	T5652	nsubj	DNA,is
R3844	T5657	T5652	neg	not,is
R3845	T5658	T5652	acomp	able,is
R3846	T5659	T5660	aux	to,activate
R3847	T5660	T5658	xcomp	activate,able
R3848	T5661	T5662	det	the,activity
R3849	T5662	T5660	dobj	activity,activate
R3850	T5663	T5662	compound	ATPase,activity
R3851	T5664	T5662	prep	of,activity
R3852	T5665	T5664	pobj	Mcm4,of
R3853	T5666	T5665	punct	/,Mcm4
R3854	T5667	T5665	nummod	6,Mcm4
R3855	T5668	T5665	punct	/,Mcm4
R3856	T5669	T5665	nummod	7,Mcm4
R3857	T5670	T5671	punct	(,10
R3858	T5671	T5652	parataxis	10,is
R3859	T5672	T5671	punct	),10
R3860	T5673	T5630	punct	.,expected
R3861	T5675	T5676	prep	In,binds
R3862	T5677	T5675	pobj	contrast,In
R3863	T5678	T5676	punct	", ",binds
R3864	T5679	T5680	det	the,PriA
R3865	T5680	T5676	nsubj	PriA,binds
R3866	T5681	T5680	punct	", ",PriA
R3867	T5682	T5683	det	a,helicase
R3868	T5683	T5680	appos	helicase,PriA
R3869	T5684	T5685	npadvmod	structure,specific
R3870	T5685	T5683	amod	specific,helicase
R3871	T5686	T5685	punct	-,specific
R3872	T5687	T5683	acl	used,helicase
R3873	T5688	T5687	prep	as,used
R3874	T5689	T5690	det	a,control
R3875	T5690	T5688	pobj	control,as
R3876	T5691	T5676	punct	", ",binds
R3877	T5692	T5676	prep	at,binds
R3878	T5693	T5694	det	the,junction
R3879	T5694	T5692	pobj	junction,at
R3880	T5695	T5694	compound	fork,junction
R3881	T5696	T5676	cc	and,binds
R3882	T5697	T5676	conj	unwinds,binds
R3883	T5698	T5699	det	the,fork
R3884	T5699	T5697	dobj	fork,unwinds
R3885	T5700	T5699	cc	and,fork
R3886	T5701	T5702	det	the,DNA
R3887	T5702	T5699	conj	DNA,fork
R3888	T5703	T5702	amod	nascent,DNA
R3889	T5704	T5702	amod	lagging,DNA
R3890	T5705	T5702	compound	strand,DNA
R3891	T5706	T5702	prep	on,DNA
R3892	T5707	T5708	compound	A,fork
R3893	T5708	T5706	pobj	fork,on
R3894	T5709	T5708	punct	-,fork
R3895	T5710	T5708	punct	[,fork
R3896	T5711	T5708	nummod	5,fork
R3897	T5712	T5708	punct	′,fork
R3898	T5713	T5676	punct	],binds
R3899	T5714	T5676	punct	", ",binds
R3900	T5715	T5676	cc	or,binds
R3901	T5716	T5676	conj	unwinds,binds
R3902	T5717	T5718	det	the,strand
R3903	T5718	T5716	dobj	strand,unwinds
R3904	T5719	T5718	amod	nascent,strand
R3905	T5720	T5718	amod	lagging,strand
R3906	T5721	T5716	prep	on,unwinds
R3907	T5722	T5723	compound	A,fork
R3908	T5723	T5721	pobj	fork,on
R3909	T5724	T5723	punct	-,fork
R3910	T5725	T5723	punct	[,fork
R3911	T5726	T5723	appos	3,fork
R3912	T5727	T5726	punct	′,3
R3913	T5728	T5726	punct	",",3
R3914	T5729	T5726	appos	5,3
R3915	T5730	T5729	punct	′,5
R3916	T5731	T5676	punct	],binds
R3917	T5732	T5676	punct	", ",binds
R3918	T5733	T5734	mark	as,reported
R3919	T5734	T5676	advcl	reported,binds
R3920	T5735	T5734	advmod	before,reported
R3921	T5736	T5737	punct	(,25
R3922	T5737	T5734	parataxis	25,reported
R3923	T5738	T5737	punct	),25
R3924	T5739	T5676	punct	.,binds
R3925	T5741	T5742	det	These,results
R3926	T5742	T5743	nsubj	results,indicate
R3927	T5744	T5745	mark	that,binds
R3928	T5745	T5743	advcl	binds,indicate
R3929	T5746	T5747	det	the,complex
R3930	T5747	T5745	nsubj	complex,binds
R3931	T5748	T5747	nmod	Mcm4,complex
R3932	T5749	T5748	punct	/,Mcm4
R3933	T5750	T5748	nummod	6,Mcm4
R3934	T5751	T5748	punct	/,Mcm4
R3935	T5752	T5748	nummod	7,Mcm4
R3936	T5753	T5745	prep	to,binds
R3937	T5754	T5755	amod	single,stranded
R3938	T5755	T5757	amod	stranded,DNA
R3939	T5756	T5755	punct	-,stranded
R3940	T5757	T5753	pobj	DNA,to
R3941	T5758	T5745	cc	and,binds
R3942	T5759	T5760	mark	that,activated
R3943	T5760	T5745	conj	activated,binds
R3944	T5761	T5762	poss	its,helicase
R3945	T5762	T5760	nsubjpass	helicase,activated
R3946	T5763	T5760	auxpass	is,activated
R3947	T5764	T5765	advmod	only,in
R3948	T5765	T5760	prep	in,activated
R3949	T5766	T5767	det	the,presence
R3950	T5767	T5765	pobj	presence,in
R3951	T5768	T5767	prep	of,presence
R3952	T5769	T5770	det	a,tail
R3953	T5770	T5768	pobj	tail,of
R3954	T5771	T5772	amod	single,stranded
R3955	T5772	T5770	amod	stranded,tail
R3956	T5773	T5772	punct	-,stranded
R3957	T5774	T5770	nummod	3,tail
R3958	T5775	T5774	punct	′,3
R3959	T5776	T5770	punct	-,tail
R3960	T5777	T5743	punct	.,indicate
R3967	T5933	T5934	nummod	3,extension
R3968	T5934	T5937	nsubjpass	extension,displaced
R3969	T5935	T5933	punct	′,3
R3970	T5936	T5934	punct	-,extension
R3971	T5938	T5934	prep	with,extension
R3972	T5939	T5940	nmod	poly,dT
R3973	T5940	T5942	nmod	dT,tail
R3974	T5941	T5940	punct	(,dT
R3975	T5942	T5938	pobj	tail,with
R3976	T5943	T5942	punct	),tail
R3977	T5944	T5937	auxpass	is,displaced
R3978	T5945	T5937	agent	by,displaced
R3979	T5946	T5945	pobj	Mcm4,by
R3980	T5947	T5946	punct	/,Mcm4
R3981	T5948	T5946	nummod	6,Mcm4
R3982	T5949	T5946	punct	/,Mcm4
R3983	T5950	T5946	nummod	7,Mcm4
R3984	T5952	T5953	nsubjpass	It,reported
R3985	T5954	T5953	aux	has,reported
R3986	T5955	T5953	auxpass	been,reported
R3987	T5956	T5957	mark	that,unwound
R3988	T5957	T5953	ccomp	unwound,reported
R3989	T5958	T5959	det	the,extension
R3990	T5959	T5957	nsubj	extension,unwound
R3991	T5960	T5959	nummod	3,extension
R3992	T5961	T5960	punct	′,3
R3993	T5962	T5959	punct	-,extension
R3994	T5963	T5957	aux	can,unwound
R3995	T5964	T5957	aux	be,unwound
R3996	T5965	T5957	agent	by,unwound
R3997	T5966	T5967	det	the,homohexamer
R3998	T5967	T5965	pobj	homohexamer,by
R3999	T5968	T5967	amod	archaeal,homohexamer
R4000	T5969	T5967	compound	Mcm,homohexamer
R4001	T5970	T5957	cc	but,unwound
R4002	T5971	T5957	conj	not,unwound
R4003	T5972	T5971	agent	by,not
R4004	T5973	T5974	nmod	fission,complexes
R4005	T5974	T5972	pobj	complexes,by
R4006	T5975	T5973	cc	and,fission
R4007	T5976	T5973	conj	budding,fission
R4008	T5977	T5974	compound	yeast,complexes
R4009	T5978	T5974	compound	Mcm,complexes
R4010	T5979	T5980	punct	(,27
R4011	T5980	T5953	parataxis	27,reported
R4012	T5981	T5980	nummod	14,27
R4013	T5982	T5980	punct	",",27
R4014	T5983	T5980	nummod	26,27
R4015	T5984	T5980	punct	",",27
R4016	T5985	T5980	punct	),27
R4017	T5986	T5953	punct	.,reported
R4018	T5988	T5989	nsubj	We,constructed
R4019	T5990	T5989	aux	have,constructed
R4020	T5991	T5992	nummod	3,extension
R4021	T5992	T5995	compound	extension,substrates
R4022	T5993	T5991	punct	′,3
R4023	T5994	T5992	punct	-,extension
R4024	T5995	T5989	dobj	substrates,constructed
R4025	T5996	T5995	acl	containing,substrates
R4026	T5997	T5998	nmod	poly,dT
R4027	T5998	T6000	nmod	dT,tails
R4028	T5999	T5998	punct	(,dT
R4029	T6000	T5996	dobj	tails,containing
R4030	T6001	T5998	punct	),dT
R4031	T6002	T5998	punct	", ",dT
R4032	T6003	T6004	nmod	poly,dA
R4033	T6004	T5998	conj	dA,dT
R4034	T6005	T6004	punct	(,dA
R4035	T6006	T6004	punct	),dA
R4036	T6007	T6004	punct	", ",dA
R4037	T6008	T6009	nmod	poly,dC
R4038	T6009	T6004	conj	dC,dA
R4039	T6010	T6009	punct	(,dC
R4040	T6011	T6009	punct	),dC
R4041	T6012	T6009	cc	and,dC
R4042	T6013	T6014	nmod	poly,dG
R4043	T6014	T6009	conj	dG,dC
R4044	T6015	T6014	punct	(,dG
R4045	T6016	T6014	punct	),dG
R4046	T6017	T6000	nummod	3,tails
R4047	T6018	T6017	punct	′,3
R4048	T6019	T6000	punct	-,tails
R4049	T6020	T5989	prep	in,constructed
R4050	T6021	T6020	pobj	order,in
R4051	T6022	T6023	aux	to,examine
R4052	T6023	T6021	acl	examine,order
R4053	T6024	T6025	mark	whether,displace
R4054	T6025	T6023	ccomp	displace,examine
R4055	T6026	T6027	compound	mouse,Mcm4
R4056	T6027	T6025	nsubj	Mcm4,displace
R4057	T6028	T6027	punct	/,Mcm4
R4058	T6029	T6027	nummod	6,Mcm4
R4059	T6030	T6027	punct	/,Mcm4
R4060	T6031	T6027	nummod	7,Mcm4
R4061	T6032	T6025	aux	can,displace
R4062	T6033	T6034	det	these,substrates
R4063	T6034	T6025	dobj	substrates,displace
R4064	T6035	T5989	punct	.,constructed
R4065	T6037	T6038	nsubj	We,found
R4066	T6039	T6038	advmod	first,found
R4067	T6040	T6041	mark	that,binds
R4068	T6041	T6038	ccomp	binds,found
R4069	T6042	T6041	nsubj	Mcm4,binds
R4070	T6043	T6042	punct	/,Mcm4
R4071	T6044	T6042	nummod	6,Mcm4
R4072	T6045	T6042	punct	/,Mcm4
R4073	T6046	T6042	nummod	7,Mcm4
R4074	T6047	T6041	prep	to,binds
R4075	T6048	T6049	nmod	dT,extension
R4076	T6049	T6047	pobj	extension,to
R4077	T6050	T6048	punct	-,dT
R4078	T6051	T6048	punct	", ",dT
R4079	T6052	T6048	conj	dC,dT
R4080	T6053	T6052	punct	-,dC
R4081	T6054	T6052	cc	and,dC
R4082	T6055	T6052	conj	dG,dC
R4083	T6056	T6049	punct	-,extension
R4084	T6057	T6049	nummod	3,extension
R4085	T6058	T6057	punct	′,3
R4086	T6059	T6049	punct	-,extension
R4087	T6060	T6041	prep	with,binds
R4088	T6061	T6062	amod	similar,affinity
R4089	T6062	T6060	pobj	affinity,with
R4090	T6063	T6041	punct	", ",binds
R4091	T6064	T6041	cc	and,binds
R4092	T6065	T6041	conj	to,binds
R4093	T6066	T6067	nmod	dA,extension
R4094	T6067	T6065	pobj	extension,to
R4095	T6068	T6067	punct	-,extension
R4096	T6069	T6067	nummod	3,extension
R4097	T6070	T6069	punct	′,3
R4098	T6071	T6067	punct	-,extension
R4099	T6072	T6065	prep	with,to
R4100	T6073	T6074	advmod	much,lower
R4101	T6074	T6075	amod	lower,affinity
R4102	T6075	T6072	pobj	affinity,with
R4103	T6076	T6077	punct	(,5A
R4104	T6077	T6065	parataxis	5A,to
R4105	T6078	T6077	compound	Figure,5A
R4106	T6079	T6077	punct	),5A
R4107	T6080	T6038	punct	.,found
R4108	T6082	T6083	nsubj	This,is
R4109	T6084	T6083	acomp	consistent,is
R4110	T6085	T6084	prep	with,consistent
R4111	T6086	T6087	det	the,results
R4112	T6087	T6085	pobj	results,with
R4113	T6088	T6087	amod	previous,results
R4114	T6089	T6090	mark	that,binds
R4115	T6090	T6087	acl	binds,results
R4116	T6091	T6090	nsubj	Mcm4,binds
R4117	T6092	T6091	punct	/,Mcm4
R4118	T6093	T6091	nummod	6,Mcm4
R4119	T6094	T6091	punct	/,Mcm4
R4120	T6095	T6091	nummod	7,Mcm4
R4121	T6096	T6090	prep	to,binds
R4122	T6097	T6098	compound	50mer,dT
R4123	T6098	T6096	pobj	dT,to
R4124	T6099	T6098	compound	oligo,dT
R4125	T6100	T6098	punct	-,dT
R4126	T6101	T6098	punct	", ",dT
R4127	T6102	T6103	punct	-,dC
R4128	T6103	T6098	conj	dC,dT
R4129	T6104	T6103	cc	and,dC
R4130	T6105	T6106	punct	-,dG
R4131	T6106	T6103	conj	dG,dC
R4132	T6107	T6096	punct	", ",to
R4133	T6108	T6096	cc	but,to
R4134	T6109	T6108	neg	not,but
R4135	T6110	T6096	conj	to,to
R4136	T6111	T6112	det	a,dA
R4137	T6112	T6110	pobj	dA,to
R4138	T6113	T6112	compound	50mer,dA
R4139	T6114	T6112	compound	oligo,dA
R4140	T6115	T6112	punct	-,dA
R4141	T6116	T6117	punct	(,13
R4142	T6117	T6083	parataxis	13,is
R4143	T6118	T6117	punct	),13
R4144	T6119	T6083	punct	.,is
R4145	T6121	T6122	nsubj	We,found
R4146	T6123	T6124	mark	that,displaced
R4147	T6124	T6122	ccomp	displaced,found
R4148	T6125	T6126	npadvmod	dT,tailed
R4149	T6126	T6128	amod	tailed,extension
R4150	T6127	T6126	punct	-,tailed
R4151	T6128	T6124	nsubjpass	extension,displaced
R4152	T6129	T6128	nummod	3,extension
R4153	T6130	T6129	punct	′,3
R4154	T6131	T6128	punct	-,extension
R4155	T6132	T6124	auxpass	was,displaced
R4156	T6133	T6124	advmod	efficiently,displaced
R4157	T6134	T6124	agent	by,displaced
R4158	T6135	T6134	pobj	Mcm,by
R4159	T6136	T6135	nummod	4,Mcm
R4160	T6137	T6135	punct	/,Mcm
R4161	T6138	T6135	nummod	6,Mcm
R4162	T6139	T6135	punct	/,Mcm
R4163	T6140	T6135	nummod	7,Mcm
R4164	T6141	T6142	mark	while,detected
R4165	T6142	T6124	advcl	detected,displaced
R4166	T6143	T6144	advmod	almost,no
R4167	T6144	T6142	nsubjpass	no,detected
R4168	T6145	T6144	cc	or,no
R4169	T6146	T6147	advmod	very,little
R4170	T6147	T6148	nmod	little,activity
R4171	T6148	T6144	conj	activity,no
R4172	T6149	T6142	auxpass	was,detected
R4173	T6150	T6142	prep	on,detected
R4174	T6151	T6152	amod	other,substrates
R4175	T6152	T6150	pobj	substrates,on
R4176	T6153	T6154	punct	(,5B
R4177	T6154	T6142	parataxis	5B,detected
R4178	T6155	T6154	compound	Figure,5B
R4179	T6156	T6154	cc	and,5B
R4180	T6157	T6154	conj	C,5B
R4181	T6158	T6154	punct	),5B
R4182	T6159	T6122	punct	.,found
R4183	T6161	T6162	nsubj	This,is
R4184	T6163	T6162	prep	in,is
R4185	T6164	T6165	amod	sharp,contrast
R4186	T6165	T6163	pobj	contrast,in
R4187	T6166	T6165	prep	to,contrast
R4188	T6167	T6168	nmod	yeast,helicases
R4189	T6168	T6166	pobj	helicases,to
R4190	T6169	T6168	nmod	Mcm4,helicases
R4191	T6170	T6169	punct	/,Mcm4
R4192	T6171	T6169	nummod	6,Mcm4
R4193	T6172	T6169	punct	/,Mcm4
R4194	T6173	T6169	nummod	7,Mcm4
R4195	T6174	T6175	dep	which,are
R4196	T6175	T6168	relcl	are,helicases
R4197	T6176	T6175	neg	not,are
R4198	T6177	T6175	acomp	capable,are
R4199	T6178	T6177	prep	of,capable
R4200	T6179	T6178	pcomp	unwinding,of
R4201	T6180	T6181	nummod	3,extension
R4202	T6181	T6184	compound	extension,substrates
R4203	T6182	T6180	punct	′,3
R4204	T6183	T6181	punct	-,extension
R4205	T6184	T6179	dobj	substrates,unwinding
R4206	T6185	T6162	punct	.,is
R4207	T6187	T6188	advmod	Furthermore,displaced
R4208	T6189	T6188	punct	", ",displaced
R4209	T6190	T6191	npadvmod	dT,tailed
R4210	T6191	T6193	amod	tailed,extension
R4211	T6192	T6191	punct	-,tailed
R4212	T6193	T6188	nsubjpass	extension,displaced
R4213	T6194	T6193	nummod	5,extension
R4214	T6195	T6194	punct	′,5
R4215	T6196	T6193	punct	-,extension
R4216	T6197	T6193	punct	", ",extension
R4217	T6198	T6199	prep	to,bind
R4218	T6199	T6193	relcl	bind,extension
R4219	T6200	T6198	pobj	which,to
R4220	T6201	T6199	nsubj	Mcm4,bind
R4221	T6202	T6201	punct	/,Mcm4
R4222	T6203	T6201	nummod	6,Mcm4
R4223	T6204	T6201	punct	/,Mcm4
R4224	T6205	T6201	nummod	7,Mcm4
R4225	T6206	T6199	aux	could,bind
R4226	T6207	T6188	punct	", ",displaced
R4227	T6208	T6188	auxpass	was,displaced
R4228	T6209	T6188	neg	not,displaced
R4229	T6210	T6188	agent	by,displaced
R4230	T6211	T6212	det	the,helicase
R4231	T6212	T6210	pobj	helicase,by
R4232	T6213	T6212	compound	Mcm,helicase
R4233	T6214	T6188	punct	.,displaced
R4234	T6216	T6217	det	The,addition
R4235	T6217	T6218	nsubj	addition,stimulated
R4236	T6219	T6217	prep	of,addition
R4237	T6220	T6221	det	the,tail
R4238	T6221	T6219	pobj	tail,of
R4239	T6222	T6221	nummod	5,tail
R4240	T6223	T6222	punct	′,5
R4241	T6224	T6221	punct	-,tail
R4242	T6225	T6217	prep	to,addition
R4243	T6226	T6227	npadvmod	dT,tailed
R4244	T6227	T6229	amod	tailed,extension
R4245	T6228	T6227	punct	-,tailed
R4246	T6229	T6225	pobj	extension,to
R4247	T6230	T6229	nummod	3,extension
R4248	T6231	T6230	punct	′,3
R4249	T6232	T6229	punct	-,extension
R4250	T6233	T6218	advmod	further,stimulated
R4251	T6234	T6235	det	the,displacement
R4252	T6235	T6218	dobj	displacement,stimulated
R4253	T6236	T6237	punct	(,5D
R4254	T6237	T6235	parataxis	5D,displacement
R4255	T6238	T6237	compound	Figure,5D
R4256	T6239	T6237	punct	),5D
R4257	T6240	T6218	punct	", ",stimulated
R4258	T6241	T6218	advcl	confirming,stimulated
R4259	T6242	T6243	det	the,report
R4260	T6243	T6241	dobj	report,confirming
R4261	T6244	T6243	amod	previous,report
R4262	T6245	T6246	mark	that,facilitate
R4263	T6246	T6243	acl	facilitate,report
R4264	T6247	T6248	det	the,structures
R4265	T6248	T6246	nsubj	structures,facilitate
R4266	T6249	T6248	compound	fork,structures
R4267	T6250	T6251	det	the,action
R4268	T6251	T6246	dobj	action,facilitate
R4269	T6252	T6251	compound	helicase,action
R4270	T6253	T6218	punct	.,stimulated
R4271	T6255	T6256	advmod	Thus,strengthen
R4272	T6257	T6256	punct	", ",strengthen
R4273	T6258	T6259	det	these,results
R4274	T6259	T6256	nsubj	results,strengthen
R4275	T6260	T6256	advmod	further,strengthen
R4276	T6261	T6262	poss	our,conclusion
R4277	T6262	T6256	dobj	conclusion,strengthen
R4278	T6263	T6264	mark	that,activated
R4279	T6264	T6262	acl	activated,conclusion
R4280	T6265	T6266	det	the,helicase
R4281	T6266	T6264	nsubjpass	helicase,activated
R4282	T6267	T6266	amod	mammalian,helicase
R4283	T6268	T6266	compound	Mcm,helicase
R4284	T6269	T6264	auxpass	is,activated
R4285	T6270	T6264	advmod	generally,activated
R4286	T6271	T6264	agent	by,activated
R4287	T6272	T6273	det	the,stretch
R4288	T6273	T6271	pobj	stretch,by
R4289	T6274	T6273	compound	thymine,stretch
R4290	T6275	T6273	punct	-,stretch
R4291	T6276	T6273	prep	on,stretch
R4292	T6277	T6278	det	the,tail
R4293	T6278	T6276	pobj	tail,on
R4294	T6279	T6278	nummod	3,tail
R4295	T6280	T6279	punct	′,3
R4296	T6281	T6278	punct	-,tail
R4297	T6282	T6283	amod	single,stranded
R4298	T6283	T6278	amod	stranded,tail
R4299	T6284	T6283	punct	-,stranded
R4300	T6285	T6256	punct	.,strengthen
R4301	T6404	T6405	det	The,effects
R4302	T6406	T6405	prep	of,effects
R4303	T6407	T6408	det	the,content
R4304	T6408	T6406	pobj	content,of
R4305	T6409	T6408	compound	thymine,content
R4306	T6410	T6408	prep	of,content
R4307	T6411	T6412	det	the,tail
R4308	T6412	T6410	pobj	tail,of
R4309	T6413	T6412	nummod	3,tail
R4310	T6414	T6413	punct	′,3
R4311	T6415	T6412	punct	-,tail
R4312	T6416	T6405	prep	on,effects
R4313	T6417	T6418	compound	Mcm,helicase
R4314	T6418	T6419	compound	helicase,activity
R4315	T6419	T6416	pobj	activity,on
R4316	T6421	T6422	nsubj	We,examined
R4317	T6423	T6422	advmod	next,examined
R4318	T6424	T6425	advmod	more,systematically
R4319	T6425	T6422	advmod	systematically,examined
R4320	T6426	T6427	det	the,effect
R4321	T6427	T6422	dobj	effect,examined
R4322	T6428	T6427	prep	of,effect
R4323	T6429	T6430	det	the,compositions
R4324	T6430	T6428	pobj	compositions,of
R4325	T6431	T6430	prep	of,compositions
R4326	T6432	T6433	det	the,residues
R4327	T6433	T6431	pobj	residues,of
R4328	T6434	T6433	compound	thymine,residues
R4329	T6435	T6433	prep	of,residues
R4330	T6436	T6437	det	the,tail
R4331	T6437	T6435	pobj	tail,of
R4332	T6438	T6437	nummod	3,tail
R4333	T6439	T6438	punct	′,3
R4334	T6440	T6437	punct	-,tail
R4335	T6441	T6427	prep	on,effect
R4336	T6442	T6443	det	the,activity
R4337	T6443	T6441	pobj	activity,on
R4338	T6444	T6445	compound	Mcm,helicase
R4339	T6445	T6443	compound	helicase,activity
R4340	T6446	T6422	punct	.,examined
R4341	T6448	T6449	det	A,DNA
R4342	T6449	T6456	nsubjpass	DNA,constructed
R4343	T6450	T6449	amod	partial,DNA
R4344	T6451	T6449	nmod	heteroduplex,DNA
R4345	T6452	T6449	nmod	M13,DNA
R4346	T6453	T6454	amod	single,stranded
R4347	T6454	T6449	amod	stranded,DNA
R4348	T6455	T6454	punct	-,stranded
R4349	T6457	T6449	acl	containing,DNA
R4350	T6458	T6459	det	a,region
R4351	T6459	T6457	dobj	region,containing
R4352	T6460	T6461	nummod	37,bp
R4353	T6461	T6459	compound	bp,region
R4354	T6462	T6459	compound	duplex,region
R4355	T6463	T6457	prep	in,containing
R4356	T6464	T6465	det	the,presence
R4357	T6465	T6463	pobj	presence,in
R4358	T6466	T6465	cc	or,presence
R4359	T6467	T6465	conj	absence,presence
R4360	T6468	T6465	prep	of,presence
R4361	T6469	T6470	det	the,tail
R4362	T6470	T6468	pobj	tail,of
R4363	T6471	T6472	nummod	50,nt
R4364	T6472	T6473	npadvmod	nt,long
R4365	T6473	T6470	amod	long,tail
R4366	T6474	T6470	nummod	3,tail
R4367	T6475	T6474	punct	′,3
R4368	T6476	T6470	punct	-,tail
R4369	T6477	T6470	prep	of,tail
R4370	T6478	T6479	amod	various,compositions
R4371	T6479	T6477	pobj	compositions,of
R4372	T6480	T6479	compound	nucleotide,compositions
R4373	T6481	T6456	auxpass	were,constructed
R4374	T6482	T6456	cc	and,constructed
R4375	T6483	T6484	det	the,unwinding
R4376	T6484	T6485	nsubjpass	unwinding,examined
R4377	T6485	T6456	conj	examined,constructed
R4378	T6486	T6484	prep	by,unwinding
R4379	T6487	T6488	compound	mouse,Mcm4
R4380	T6488	T6486	pobj	Mcm4,by
R4381	T6489	T6488	punct	/,Mcm4
R4382	T6490	T6488	nummod	6,Mcm4
R4383	T6491	T6488	punct	/,Mcm4
R4384	T6492	T6488	nummod	7,Mcm4
R4385	T6493	T6485	auxpass	was,examined
R4386	T6494	T6495	punct	(,6A
R4387	T6495	T6485	parataxis	6A,examined
R4388	T6496	T6495	compound	Figure,6A
R4389	T6497	T6495	punct	),6A
R4390	T6498	T6485	punct	.,examined
R4391	T6500	T6501	prep	Due,displaced
R4392	T6502	T6500	pcomp	to,Due
R4393	T6503	T6504	poss	its,processivity
R4394	T6504	T6500	pobj	processivity,Due
R4395	T6505	T6504	amod	low,processivity
R4396	T6506	T6501	punct	", ",displaced
R4397	T6507	T6501	nsubj	Mcm4,displaced
R4398	T6508	T6507	punct	/,Mcm4
R4399	T6509	T6507	nummod	6,Mcm4
R4400	T6510	T6507	punct	/,Mcm4
R4401	T6511	T6507	nummod	7,Mcm4
R4402	T6512	T6501	advmod	barely,displaced
R4403	T6513	T6514	det	the,oligonucleotide
R4404	T6514	T6501	dobj	oligonucleotide,displaced
R4405	T6515	T6514	amod	annealed,oligonucleotide
R4406	T6516	T6514	compound	37mer,oligonucleotide
R4407	T6517	T6514	prep	without,oligonucleotide
R4408	T6518	T6519	det	a,tail
R4409	T6519	T6517	pobj	tail,without
R4410	T6520	T6519	nummod	3,tail
R4411	T6521	T6520	punct	′,3
R4412	T6522	T6519	punct	-,tail
R4413	T6523	T6524	punct	(,6A
R4414	T6524	T6514	parataxis	6A,oligonucleotide
R4415	T6525	T6524	dep	37mer,6A
R4416	T6526	T6524	punct	;,6A
R4417	T6527	T6524	compound	Figure,6A
R4418	T6528	T6524	punct	),6A
R4419	T6529	T6501	punct	.,displaced
R4420	T6531	T6532	advmod	Only,activity
R4421	T6532	T6535	nsubjpass	activity,observed
R4422	T6533	T6532	amod	weak,activity
R4423	T6534	T6532	compound	helicase,activity
R4424	T6536	T6535	auxpass	was,observed
R4425	T6537	T6535	prep	on,observed
R4426	T6538	T6539	det	the,substrate
R4427	T6539	T6537	pobj	substrate,on
R4428	T6540	T6539	acl	carrying,substrate
R4429	T6541	T6542	det	the,tail
R4430	T6542	T6540	dobj	tail,carrying
R4431	T6543	T6542	nummod	3,tail
R4432	T6544	T6543	punct	′,3
R4433	T6545	T6542	punct	-,tail
R4434	T6546	T6542	prep	of,tail
R4435	T6547	T6548	nummod	25,repeats
R4436	T6548	T6546	pobj	repeats,of
R4437	T6549	T6548	prep	of,repeats
R4438	T6550	T6551	compound	TA,dinucleotide
R4439	T6551	T6549	pobj	dinucleotide,of
R4440	T6552	T6553	punct	(,6A
R4441	T6553	T6540	parataxis	6A,carrying
R4442	T6554	T6555	nummod	37,TA25
R4443	T6555	T6553	dep	TA25,6A
R4444	T6556	T6555	punct	-,TA25
R4445	T6557	T6553	punct	;,6A
R4446	T6558	T6553	compound	Figure,6A
R4447	T6559	T6553	punct	),6A
R4448	T6560	T6535	punct	", ",observed
R4449	T6561	T6562	mark	whereas,observed
R4450	T6562	T6535	advcl	observed,observed
R4451	T6563	T6564	amod	strong,activity
R4452	T6564	T6562	nsubjpass	activity,observed
R4453	T6565	T6564	compound	helicase,activity
R4454	T6566	T6562	auxpass	was,observed
R4455	T6567	T6562	prep	on,observed
R4456	T6568	T6569	nummod	37,TTA17
R4457	T6569	T6567	pobj	TTA17,on
R4458	T6570	T6569	punct	-,TTA17
R4459	T6571	T6535	punct	.,observed
R4460	T6573	T6574	nsubj	Increase,increase
R4461	T6575	T6573	prep	of,Increase
R4462	T6576	T6577	compound	thymine,content
R4463	T6577	T6575	pobj	content,of
R4464	T6578	T6573	prep	on,Increase
R4465	T6579	T6580	det	the,tail
R4466	T6580	T6578	pobj	tail,on
R4467	T6581	T6580	nummod	3,tail
R4468	T6582	T6581	punct	′,3
R4469	T6583	T6580	punct	-,tail
R4470	T6584	T6574	aux	did,increase
R4471	T6585	T6574	neg	not,increase
R4472	T6586	T6574	advmod	further,increase
R4473	T6587	T6588	det	the,extent
R4474	T6588	T6574	dobj	extent,increase
R4475	T6589	T6588	prep	of,extent
R4476	T6590	T6589	pobj	unwinding,of
R4477	T6591	T6592	punct	(,6A
R4478	T6592	T6590	parataxis	6A,unwinding
R4479	T6593	T6594	nummod	37,TTTA13
R4480	T6594	T6592	dep	TTTA13,6A
R4481	T6595	T6594	punct	-,TTTA13
R4482	T6596	T6594	punct	", ",TTTA13
R4483	T6597	T6598	nummod	37,TTTTA10
R4484	T6598	T6594	conj	TTTTA10,TTTA13
R4485	T6599	T6598	punct	-,TTTTA10
R4486	T6600	T6598	cc	and,TTTTA10
R4487	T6601	T6602	nummod	37,T50
R4488	T6602	T6598	conj	T50,TTTTA10
R4489	T6603	T6602	punct	-,T50
R4490	T6604	T6592	punct	;,6A
R4491	T6605	T6592	compound	Figure,6A
R4492	T6606	T6592	punct	),6A
R4493	T6607	T6574	punct	", ",increase
R4494	T6608	T6574	advcl	suggesting,increase
R4495	T6609	T6610	mark	that,be
R4496	T6610	T6608	ccomp	be,suggesting
R4497	T6611	T6612	nummod	17,repeats
R4498	T6612	T6610	nsubj	repeats,be
R4499	T6613	T6612	prep	of,repeats
R4500	T6614	T6615	compound	TT,dinucleotides
R4501	T6615	T6613	pobj	dinucleotides,of
R4502	T6616	T6617	punct	(,thymine
R4503	T6617	T6615	parataxis	thymine,dinucleotides
R4504	T6618	T6619	nummod	67,%
R4505	T6619	T6617	compound	%,thymine
R4506	T6620	T6617	punct	),thymine
R4507	T6621	T6610	aux	may,be
R4508	T6622	T6610	acomp	sufficient,be
R4509	T6623	T6622	prep	for,sufficient
R4510	T6624	T6623	pobj	activation,for
R4511	T6625	T6624	prep	of,activation
R4512	T6626	T6627	compound	Mcm,helicase
R4513	T6627	T6625	pobj	helicase,of
R4514	T6628	T6574	punct	.,increase
R4515	T6630	T6631	nsubjpass	It,noted
R4516	T6632	T6631	aux	should,noted
R4517	T6633	T6631	auxpass	be,noted
R4518	T6634	T6635	mark	that,anneal
R4519	T6635	T6631	ccomp	anneal,noted
R4520	T6636	T6637	det	the,tail
R4521	T6637	T6635	nsubj	tail,anneal
R4522	T6638	T6637	compound	TA25,tail
R4523	T6639	T6635	aux	would,anneal
R4524	T6640	T6635	dep	self,anneal
R4525	T6641	T6635	punct	-,anneal
R4526	T6642	T6635	cc	and,anneal
R4527	T6643	T6644	nsubj	this,prevent
R4528	T6644	T6635	conj	prevent,anneal
R4529	T6645	T6644	aux	would,prevent
R4530	T6646	T6647	det	the,loading
R4531	T6647	T6644	dobj	loading,prevent
R4532	T6648	T6647	prep	of,loading
R4533	T6649	T6648	pobj	Mcm,of
R4534	T6650	T6631	punct	", ",noted
R4535	T6651	T6652	mark	since,enhance
R4536	T6652	T6631	advcl	enhance,noted
R4537	T6653	T6654	det	the,tail
R4538	T6654	T6652	nsubj	tail,enhance
R4539	T6655	T6654	nummod	3,tail
R4540	T6656	T6655	punct	′,3
R4541	T6657	T6654	punct	-,tail
R4542	T6658	T6654	acl	containing,tail
R4543	T6659	T6658	dobj	TTAA13,containing
R4544	T6660	T6659	cc	or,TTAA13
R4545	T6661	T6659	conj	TTTAAA8,TTAA13
R4546	T6662	T6652	aux	did,enhance
R4547	T6663	T6652	neg	not,enhance
R4548	T6664	T6665	det	the,unwinding
R4549	T6665	T6652	dobj	unwinding,enhance
R4550	T6666	T6652	advmod	either,enhance
R4551	T6667	T6668	punct	(,shown
R4552	T6668	T6652	parataxis	shown,enhance
R4553	T6669	T6668	nsubj	data,shown
R4554	T6670	T6668	neg	not,shown
R4555	T6671	T6668	punct	),shown
R4556	T6672	T6631	punct	.,noted
R4557	T6674	T6675	advmod	Therefore,examined
R4558	T6676	T6675	punct	", ",examined
R4559	T6677	T6675	nsubj	we,examined
R4560	T6678	T6675	advmod	next,examined
R4561	T6679	T6680	det	the,effects
R4562	T6680	T6675	dobj	effects,examined
R4563	T6681	T6680	prep	of,effects
R4564	T6682	T6683	amod	repeating,sequences
R4565	T6683	T6681	pobj	sequences,of
R4566	T6684	T6683	prep	of,sequences
R4567	T6685	T6684	pobj	thymine,of
R4568	T6686	T6685	cc	and,thymine
R4569	T6687	T6685	conj	cytosine,thymine
R4570	T6688	T6689	punct	(,6B
R4571	T6689	T6675	parataxis	6B,examined
R4572	T6690	T6689	compound	Figure,6B
R4573	T6691	T6689	punct	),6B
R4574	T6692	T6675	punct	.,examined
R4575	T6694	T6695	amod	Partial,substrates
R4576	T6695	T6697	nsubjpass	substrates,displaced
R4577	T6696	T6695	compound	heteroduplex,substrates
R4578	T6698	T6695	acl	containing,substrates
R4579	T6699	T6700	det	the,tail
R4580	T6700	T6698	dobj	tail,containing
R4581	T6701	T6700	nummod	3,tail
R4582	T6702	T6701	punct	′,3
R4583	T6703	T6700	punct	-,tail
R4584	T6704	T6700	prep	of,tail
R4585	T6705	T6706	nmod	TC25,TTCC17
R4586	T6706	T6704	pobj	TTCC17,of
R4587	T6707	T6706	punct	", ",TTCC17
R4588	T6708	T6706	nmod	TTCC13,TTCC17
R4589	T6709	T6706	punct	", ",TTCC17
R4590	T6710	T6706	nmod	TTTCCC8,TTCC17
R4591	T6711	T6706	punct	", ",TTCC17
R4592	T6712	T6706	nmod	TTTTCCCC6,TTCC17
R4593	T6713	T6706	punct	", ",TTCC17
R4594	T6714	T6697	auxpass	were,displaced
R4595	T6715	T6697	agent	by,displaced
R4596	T6716	T6717	det	the,complex
R4597	T6717	T6715	pobj	complex,by
R4598	T6718	T6717	nmod	Mcm4,complex
R4599	T6719	T6718	punct	/,Mcm4
R4600	T6720	T6718	nummod	6,Mcm4
R4601	T6721	T6718	punct	/,Mcm4
R4602	T6722	T6718	nummod	7,Mcm4
R4603	T6723	T6697	prep	to,displaced
R4604	T6724	T6725	det	the,extent
R4605	T6725	T6723	pobj	extent,to
R4606	T6726	T6725	amod	similar,extent
R4607	T6727	T6726	prep	to,similar
R4608	T6728	T6727	pobj	that,to
R4609	T6729	T6728	acl	achieved,that
R4610	T6730	T6729	agent	by,achieved
R4611	T6731	T6730	pobj	TTA17,by
R4612	T6732	T6697	punct	", ",displaced
R4613	T6733	T6697	advcl	suggesting,displaced
R4614	T6734	T6735	mark	that,is
R4615	T6735	T6733	ccomp	is,suggesting
R4616	T6736	T6737	nummod	50,%
R4617	T6737	T6738	compound	%,content
R4618	T6738	T6735	nsubj	content,is
R4619	T6739	T6738	compound	thymine,content
R4620	T6740	T6735	acomp	sufficient,is
R4621	T6741	T6740	prep	for,sufficient
R4622	T6742	T6743	amod	full,activation
R4623	T6743	T6741	pobj	activation,for
R4624	T6744	T6743	prep	of,activation
R4625	T6745	T6746	compound	Mcm,helicase
R4626	T6746	T6744	pobj	helicase,of
R4627	T6747	T6697	punct	.,displaced
R4628	T6749	T6750	nsubjpass	TTCC13,displaced
R4629	T6751	T6749	cc	and,TTCC13
R4630	T6752	T6749	conj	TTCC17,TTCC13
R4631	T6753	T6750	auxpass	were,displaced
R4632	T6754	T6750	prep	to,displaced
R4633	T6755	T6756	det	a,extent
R4634	T6756	T6754	pobj	extent,to
R4635	T6757	T6756	amod	similar,extent
R4636	T6758	T6750	punct	", ",displaced
R4637	T6759	T6750	advcl	consistent,displaced
R4638	T6760	T6759	prep	with,consistent
R4639	T6761	T6762	det	the,result
R4640	T6762	T6760	pobj	result,with
R4641	T6763	T6762	amod	earlier,result
R4642	T6764	T6765	mark	that,is
R4643	T6765	T6762	acl	is,result
R4644	T6766	T6767	det	a,tail
R4645	T6767	T6765	nsubj	tail,is
R4646	T6768	T6769	nummod	40,nt
R4647	T6769	T6767	compound	nt,tail
R4648	T6770	T6765	acomp	sufficient,is
R4649	T6771	T6770	prep	for,sufficient
R4650	T6772	T6771	pobj	activation,for
R4651	T6773	T6774	punct	(,13
R4652	T6774	T6765	parataxis	13,is
R4653	T6775	T6774	punct	),13
R4654	T6776	T6750	punct	.,displaced
R4655	T6778	T6779	advmod	However,decreased
R4656	T6780	T6779	punct	", ",decreased
R4657	T6781	T6782	det	the,efficiency
R4658	T6782	T6779	nsubj	efficiency,decreased
R4659	T6783	T6782	prep	of,efficiency
R4660	T6784	T6785	det	the,displacement
R4661	T6785	T6783	pobj	displacement,of
R4662	T6786	T6779	advmod	significantly,decreased
R4663	T6787	T6788	mark	as,dropped
R4664	T6788	T6779	advcl	dropped,decreased
R4665	T6789	T6790	det	the,content
R4666	T6790	T6788	nsubj	content,dropped
R4667	T6791	T6790	compound	thymine,content
R4668	T6792	T6790	prep	of,content
R4669	T6793	T6794	det	the,tail
R4670	T6794	T6792	pobj	tail,of
R4671	T6795	T6794	nummod	3,tail
R4672	T6796	T6795	punct	′,3
R4673	T6797	T6794	punct	-,tail
R4674	T6798	T6788	prep	to,dropped
R4675	T6799	T6800	nummod	33,%
R4676	T6800	T6798	pobj	%,to
R4677	T6801	T6788	punct	", ",dropped
R4678	T6802	T6788	prep	as,dropped
R4679	T6803	T6802	prep	in,as
R4680	T6804	T6803	pobj	TAA17,in
R4681	T6805	T6804	cc	or,TAA17
R4682	T6806	T6804	conj	TCC17,TAA17
R4683	T6807	T6808	punct	(,6B
R4684	T6808	T6788	parataxis	6B,dropped
R4685	T6809	T6808	compound	Figure,6B
R4686	T6810	T6808	cc	and,6B
R4687	T6811	T6808	conj	C,6B
R4688	T6812	T6808	punct	),6B
R4689	T6813	T6779	punct	.,decreased
R4690	T6815	T6816	advmod	Thus,be
R4691	T6817	T6816	punct	", ",be
R4692	T6818	T6819	det	the,content
R4693	T6819	T6816	nsubj	content,be
R4694	T6820	T6819	amod	minimal,content
R4695	T6821	T6819	compound	thymine,content
R4696	T6822	T6819	acl	required,content
R4697	T6823	T6822	prep	for,required
R4698	T6824	T6823	pobj	activation,for
R4699	T6825	T6824	prep	of,activation
R4700	T6826	T6827	det	the,helicase
R4701	T6827	T6825	pobj	helicase,of
R4702	T6828	T6827	compound	Mcm,helicase
R4703	T6829	T6816	aux	may,be
R4704	T6830	T6816	advmod	somewhere,be
R4705	T6831	T6830	prep	between,somewhere
R4706	T6832	T6833	nummod	33,%
R4707	T6833	T6831	pobj	%,between
R4708	T6834	T6832	cc	and,33
R4709	T6835	T6832	conj	50,33
R4710	T6836	T6816	punct	.,be
R4711	T7008	T7009	compound	DUE,sequences
R4712	T7009	T7010	nsubj	sequences,activate
R4713	T7011	T7009	prep	from,sequences
R4714	T7012	T7013	det	the,origin
R4715	T7013	T7011	pobj	origin,from
R4716	T7014	T7015	compound	c,myc
R4717	T7015	T7013	compound	myc,origin
R4718	T7016	T7015	punct	-,myc
R4719	T7017	T7018	compound	Mcm,helicase
R4720	T7018	T7010	dobj	helicase,activate
R4721	T7020	T7021	poss	Our,studies
R4722	T7021	T7023	nsubj	studies,shown
R4723	T7022	T7021	amod	previous,studies
R4724	T7024	T7023	aux	have,shown
R4725	T7025	T7026	mark	that,serve
R4726	T7026	T7023	ccomp	serve,shown
R4727	T7027	T7028	det	the,sequences
R4728	T7028	T7026	nsubj	sequences,serve
R4729	T7029	T7028	acl	containing,sequences
R4730	T7030	T7031	amod	periodic,tracts
R4731	T7031	T7029	dobj	tracts,containing
R4732	T7032	T7033	punct	(,n
R4733	T7033	T7031	compound	n,tracts
R4734	T7034	T7033	nmod	dT,n
R4735	T7035	T7033	punct	),n
R4736	T7036	T7031	acl	derived,tracts
R4737	T7037	T7036	prep	from,derived
R4738	T7038	T7039	det	the,origin
R4739	T7039	T7037	pobj	origin,from
R4740	T7040	T7039	amod	human,origin
R4741	T7041	T7042	compound	lamin,B2
R4742	T7042	T7039	compound	B2,origin
R4743	T7043	T7044	punct	(,content
R4744	T7044	T7039	parataxis	content,origin
R4745	T7045	T7046	nummod	48,%
R4746	T7046	T7044	compound	%,content
R4747	T7047	T7044	compound	thymine,content
R4748	T7048	T7044	punct	),content
R4749	T7049	T7026	aux	can,serve
R4750	T7050	T7026	prep	as,serve
R4751	T7051	T7052	det	an,activator
R4752	T7052	T7050	pobj	activator,as
R4753	T7053	T7052	amod	efficient,activator
R4754	T7054	T7052	prep	for,activator
R4755	T7055	T7056	det	the,helicase
R4756	T7056	T7054	pobj	helicase,for
R4757	T7057	T7056	compound	Mcm,helicase
R4758	T7058	T7026	prep	on,serve
R4759	T7059	T7060	det	a,bubble
R4760	T7060	T7058	pobj	bubble,on
R4761	T7061	T7023	punct	", ",shown
R4762	T7062	T7023	cc	and,shown
R4763	T7063	T7064	nsubj	replacement,abolished
R4764	T7064	T7023	conj	abolished,shown
R4765	T7065	T7063	prep	of,replacement
R4766	T7066	T7065	pobj	thymines,of
R4767	T7067	T7063	prep	with,replacement
R4768	T7068	T7067	pobj	guanines,with
R4769	T7069	T7070	det	the,activation
R4770	T7070	T7064	dobj	activation,abolished
R4771	T7071	T7070	compound	helicase,activation
R4772	T7072	T7073	punct	(,28
R4773	T7073	T7064	parataxis	28,abolished
R4774	T7074	T7073	nummod	13,28
R4775	T7075	T7073	punct	",",28
R4776	T7076	T7073	punct	),28
R4777	T7077	T7064	punct	.,abolished
R4778	T7079	T7080	prep	In,constructed
R4779	T7081	T7079	pobj	order,In
R4780	T7082	T7083	aux	to,examine
R4781	T7083	T7081	acl	examine,order
R4782	T7084	T7085	amod	other,origins
R4783	T7085	T7083	dobj	origins,examine
R4784	T7086	T7085	amod	natural,origins
R4785	T7087	T7085	compound	replication,origins
R4786	T7088	T7085	prep	with,origins
R4787	T7089	T7090	amod	different,contents
R4788	T7090	T7088	pobj	contents,with
R4789	T7091	T7090	compound	thymine,contents
R4790	T7092	T7083	prep	for,examine
R4791	T7093	T7094	det	the,ability
R4792	T7094	T7092	pobj	ability,for
R4793	T7095	T7096	aux	to,activate
R4794	T7096	T7094	acl	activate,ability
R4795	T7097	T7098	compound	Mcm,helicase
R4796	T7098	T7096	dobj	helicase,activate
R4797	T7099	T7096	prep	on,activate
R4798	T7100	T7101	det	a,substrate
R4799	T7101	T7099	pobj	substrate,on
R4800	T7102	T7101	compound	bubble,substrate
R4801	T7103	T7080	punct	", ",constructed
R4802	T7104	T7080	nsubj	we,constructed
R4803	T7105	T7106	amod	new,substrates
R4804	T7106	T7080	dobj	substrates,constructed
R4805	T7107	T7106	compound	bubble,substrates
R4806	T7108	T7106	punct	", ",substrates
R4807	T7109	T7110	compound	Bub82,myc
R4808	T7110	T7106	appos	myc,substrates
R4809	T7111	T7110	punct	/,myc
R4810	T7112	T7110	compound	c,myc
R4811	T7113	T7110	punct	-,myc
R4812	T7114	T7106	punct	", ",substrates
R4813	T7115	T7106	acl	containing,substrates
R4814	T7116	T7115	dobj	sequences,containing
R4815	T7117	T7116	acl	derived,sequences
R4816	T7118	T7117	prep	from,derived
R4817	T7119	T7120	det	the,region
R4818	T7120	T7118	pobj	region,from
R4819	T7121	T7120	nmod	DUE,region
R4820	T7122	T7121	punct	(,DUE
R4821	T7123	T7124	compound	DNA,element
R4822	T7124	T7121	appos	element,DUE
R4823	T7125	T7124	compound	unwinding,element
R4824	T7126	T7120	punct	),region
R4825	T7127	T7120	prep	of,region
R4826	T7128	T7129	det	the,origin
R4827	T7129	T7127	pobj	origin,of
R4828	T7130	T7129	amod	human,origin
R4829	T7131	T7132	compound	c,myc
R4830	T7132	T7129	compound	myc,origin
R4831	T7133	T7132	punct	-,myc
R4832	T7134	T7135	dep	which,is
R4833	T7135	T7120	relcl	is,region
R4834	T7136	T7135	acomp	essential,is
R4835	T7137	T7136	prep	for,essential
R4836	T7138	T7139	compound	c,myc
R4837	T7139	T7141	compound	myc,activity
R4838	T7140	T7139	punct	-,myc
R4839	T7141	T7137	pobj	activity,for
R4840	T7142	T7141	compound	replicator,activity
R4841	T7143	T7144	punct	(,30
R4842	T7144	T7135	parataxis	30,is
R4843	T7145	T7144	nummod	29,30
R4844	T7146	T7144	punct	",",30
R4845	T7147	T7144	punct	),30
R4846	T7148	T7080	punct	.,constructed
R4847	T7150	T7151	det	The,segment
R4848	T7151	T7153	nsubj	segment,contains
R4849	T7152	T7151	amod	unpaired,segment
R4850	T7154	T7151	prep	in,segment
R4851	T7155	T7156	compound	Bub82,DUE
R4852	T7156	T7154	pobj	DUE,in
R4853	T7157	T7156	punct	/,DUE
R4854	T7158	T7159	compound	c,myc
R4855	T7159	T7156	compound	myc,DUE
R4856	T7160	T7159	punct	-,myc
R4857	T7161	T7156	punct	/,DUE
R4858	T7162	T7163	nummod	one,strand
R4859	T7163	T7153	dobj	strand,contains
R4860	T7164	T7163	prep	of,strand
R4861	T7165	T7164	pobj	DUE,of
R4862	T7166	T7153	cc	and,contains
R4863	T7167	T7168	nsubj	that,contains
R4864	T7168	T7153	conj	contains,contains
R4865	T7169	T7167	prep	in,that
R4866	T7170	T7171	compound	Bub82,C
R4867	T7171	T7169	pobj	C,in
R4868	T7172	T7171	punct	/,C
R4869	T7173	T7174	compound	c,myc
R4870	T7174	T7171	compound	myc,C
R4871	T7175	T7174	punct	-,myc
R4872	T7176	T7171	punct	/,C
R4873	T7177	T7171	compound	DUE,C
R4874	T7178	T7171	punct	-,C
R4875	T7179	T7180	det	another,strand
R4876	T7180	T7168	dobj	strand,contains
R4877	T7181	T7168	punct	.,contains
R4878	T7183	T7184	det	This,DUE
R4879	T7184	T7185	nsubjpass	DUE,believed
R4880	T7186	T7185	auxpass	is,believed
R4881	T7187	T7188	aux	to,coincide
R4882	T7188	T7185	xcomp	coincide,believed
R4883	T7189	T7188	prep	with,coincide
R4884	T7190	T7191	det	the,region
R4885	T7191	T7189	pobj	region,with
R4886	T7192	T7193	advmod	initially,unwound
R4887	T7193	T7191	amod	unwound,region
R4888	T7194	T7191	prep	of,region
R4889	T7195	T7196	det	the,origin
R4890	T7196	T7194	pobj	origin,of
R4891	T7197	T7198	compound	c,myc
R4892	T7198	T7196	compound	myc,origin
R4893	T7199	T7198	punct	-,myc
R4894	T7200	T7201	punct	(,29
R4895	T7201	T7188	parataxis	29,coincide
R4896	T7202	T7201	punct	),29
R4897	T7203	T7185	punct	", ",believed
R4898	T7204	T7185	cc	and,believed
R4899	T7205	T7206	poss	its,deletion
R4900	T7206	T7207	nsubj	deletion,reduced
R4901	T7207	T7185	conj	reduced,believed
R4902	T7208	T7207	advmod	substantially,reduced
R4903	T7209	T7210	det	the,activity
R4904	T7210	T7207	dobj	activity,reduced
R4905	T7211	T7210	compound	replicator,activity
R4906	T7212	T7213	punct	(,30
R4907	T7213	T7207	parataxis	30,reduced
R4908	T7214	T7213	punct	),30
R4909	T7215	T7207	punct	.,reduced
R4910	T7217	T7218	nsubj	Mcm4,unwound
R4911	T7219	T7217	punct	/,Mcm4
R4912	T7220	T7217	nummod	6,Mcm4
R4913	T7221	T7217	punct	/,Mcm4
R4914	T7222	T7217	nummod	7,Mcm4
R4915	T7223	T7224	compound	Bub82,B2
R4916	T7224	T7218	dobj	B2,unwound
R4917	T7225	T7224	punct	/,B2
R4918	T7226	T7224	compound	lamin,B2
R4919	T7227	T7228	punct	(,%
R4920	T7228	T7224	parataxis	%,B2
R4921	T7229	T7228	nmod	thymine,%
R4922	T7230	T7228	nummod	48,%
R4923	T7231	T7228	punct	),%
R4924	T7232	T7224	punct	", ",B2
R4925	T7233	T7234	compound	Bub82,DUE
R4926	T7234	T7224	conj	DUE,B2
R4927	T7235	T7234	punct	/,DUE
R4928	T7236	T7237	compound	c,myc
R4929	T7237	T7234	compound	myc,DUE
R4930	T7238	T7237	punct	-,myc
R4931	T7239	T7234	punct	/,DUE
R4932	T7240	T7241	punct	(,%
R4933	T7241	T7234	parataxis	%,DUE
R4934	T7242	T7241	nummod	37,%
R4935	T7243	T7241	punct	),%
R4936	T7244	T7234	cc	and,DUE
R4937	T7245	T7246	compound	Bub82,C
R4938	T7246	T7234	conj	C,DUE
R4939	T7247	T7246	punct	/,C
R4940	T7248	T7249	compound	c,myc
R4941	T7249	T7246	compound	myc,C
R4942	T7250	T7249	punct	-,myc
R4943	T7251	T7246	punct	/,C
R4944	T7252	T7246	compound	DUE,C
R4945	T7253	T7246	punct	-,C
R4946	T7254	T7255	punct	(,%
R4947	T7255	T7246	parataxis	%,C
R4948	T7256	T7255	nummod	39,%
R4949	T7257	T7255	punct	),%
R4950	T7258	T7218	prep	with,unwound
R4951	T7259	T7260	amod	similar,efficiency
R4952	T7260	T7258	pobj	efficiency,with
R4953	T7261	T7262	punct	(,7A
R4954	T7262	T7260	parataxis	7A,efficiency
R4955	T7263	T7262	compound	Figure,7A
R4956	T7264	T7262	punct	),7A
R4957	T7265	T7218	punct	", ",unwound
R4958	T7266	T7218	advcl	indicating,unwound
R4959	T7267	T7268	mark	that,serve
R4960	T7268	T7266	ccomp	serve,indicating
R4961	T7269	T7270	det	the,sequences
R4962	T7270	T7268	nsubj	sequences,serve
R4963	T7271	T7270	prep	from,sequences
R4964	T7272	T7273	det	the,origin
R4965	T7273	T7271	pobj	origin,from
R4966	T7274	T7275	compound	c,myc
R4967	T7275	T7273	compound	myc,origin
R4968	T7276	T7275	punct	-,myc
R4969	T7277	T7268	advmod	also,serve
R4970	T7278	T7268	aux	can,serve
R4971	T7279	T7268	prep	as,serve
R4972	T7280	T7281	amod	efficient,activators
R4973	T7281	T7279	pobj	activators,as
R4974	T7282	T7281	prep	of,activators
R4975	T7283	T7284	det	the,helicase
R4976	T7284	T7282	pobj	helicase,of
R4977	T7285	T7284	compound	Mcm,helicase
R4978	T7286	T7218	punct	.,unwound
R4979	T7288	T7289	det	This,result
R4980	T7289	T7290	nsubj	result,indicates
R4981	T7291	T7290	advmod	also,indicates
R4982	T7292	T7293	mark	that,activate
R4983	T7293	T7290	ccomp	activate,indicates
R4984	T7294	T7293	nsubj	sequences,activate
R4985	T7295	T7294	prep	with,sequences
R4986	T7296	T7297	nummod	37,%
R4987	T7297	T7298	compound	%,content
R4988	T7298	T7295	pobj	content,with
R4989	T7299	T7298	compound	thymine,content
R4990	T7300	T7293	aux	can,activate
R4991	T7301	T7293	dobj	Mcm4,activate
R4992	T7302	T7301	punct	/,Mcm4
R4993	T7303	T7301	nummod	6,Mcm4
R4994	T7304	T7301	punct	/,Mcm4
R4995	T7305	T7301	nummod	7,Mcm4
R4996	T7306	T7307	aux	to,displace
R4997	T7307	T7293	advcl	displace,activate
R4998	T7308	T7309	nummod	24,nt
R4999	T7309	T7310	compound	nt,duplex
R5000	T7310	T7307	dobj	duplex,displace
R5001	T7311	T7307	prep	on,displace
R5002	T7312	T7313	det	both,sides
R5003	T7313	T7311	pobj	sides,on
R5004	T7314	T7307	prep	in,displace
R5005	T7315	T7316	det	a,substrate
R5006	T7316	T7314	pobj	substrate,in
R5007	T7317	T7316	compound	bubble,substrate
R5008	T7318	T7293	punct	", ",activate
R5009	T7319	T7293	advcl	suggesting,activate
R5010	T7320	T7321	mark	that,affect
R5011	T7321	T7319	ccomp	affect,suggesting
R5012	T7322	T7323	det	the,content
R5013	T7323	T7321	nsubj	content,affect
R5014	T7324	T7323	preconj	not,content
R5015	T7325	T7324	advmod	only,not
R5016	T7326	T7323	compound	thymine,content
R5017	T7327	T7323	cc	but,content
R5018	T7328	T7327	advmod	also,but
R5019	T7329	T7330	det	the,context
R5020	T7330	T7323	conj	context,content
R5021	T7331	T7330	compound	sequence,context
R5022	T7332	T7321	aux	may,affect
R5023	T7333	T7334	det	the,activation
R5024	T7334	T7321	dobj	activation,affect
R5025	T7335	T7336	compound	Mcm,helicase
R5026	T7336	T7334	compound	helicase,activation
R5027	T7337	T7290	punct	.,indicates
R5028	T7339	T7340	prep	In,bound
R5029	T7341	T7342	compound	gel,shift
R5030	T7342	T7343	compound	shift,assays
R5031	T7343	T7339	pobj	assays,In
R5032	T7344	T7340	punct	", ",bound
R5033	T7345	T7340	nsubj	MCM4,bound
R5034	T7346	T7345	punct	/,MCM4
R5035	T7347	T7345	nummod	6,MCM4
R5036	T7348	T7345	punct	/,MCM4
R5037	T7349	T7345	nummod	7,MCM4
R5038	T7350	T7340	prep	to,bound
R5039	T7351	T7352	predet	all,bubbles
R5040	T7352	T7350	pobj	bubbles,to
R5041	T7353	T7352	det	the,bubbles
R5042	T7354	T7352	nummod	three,bubbles
R5043	T7355	T7340	punct	", ",bound
R5044	T7356	T7357	mark	although,is
R5045	T7357	T7340	advcl	is,bound
R5046	T7358	T7359	det	the,affinity
R5047	T7359	T7357	nsubj	affinity,is
R5048	T7360	T7359	prep	to,affinity
R5049	T7361	T7362	compound	Bub82,DUE
R5050	T7362	T7360	pobj	DUE,to
R5051	T7363	T7362	punct	/,DUE
R5052	T7364	T7365	compound	c,myc
R5053	T7365	T7362	compound	myc,DUE
R5054	T7366	T7365	punct	-,myc
R5055	T7367	T7362	punct	/,DUE
R5056	T7368	T7360	cc	and,to
R5057	T7369	T7360	conj	to,to
R5058	T7370	T7371	compound	Bub82,C
R5059	T7371	T7369	pobj	C,to
R5060	T7372	T7371	punct	/,C
R5061	T7373	T7374	compound	c,myc
R5062	T7374	T7371	compound	myc,C
R5063	T7375	T7374	punct	-,myc
R5064	T7376	T7371	punct	/,C
R5065	T7377	T7371	compound	DUE,C
R5066	T7378	T7371	punct	-,C
R5067	T7379	T7380	advmod	slightly,lower
R5068	T7380	T7357	acomp	lower,is
R5069	T7381	T7380	prep	than,lower
R5070	T7382	T7381	pobj	that,than
R5071	T7383	T7382	prep	to,that
R5072	T7384	T7385	nmod	Bub,B2
R5073	T7385	T7383	pobj	B2,to
R5074	T7386	T7385	punct	-,B2
R5075	T7387	T7385	nummod	82,B2
R5076	T7388	T7385	punct	/,B2
R5077	T7389	T7385	compound	lamin,B2
R5078	T7390	T7391	punct	(,7B
R5079	T7391	T7357	parataxis	7B,is
R5080	T7392	T7391	compound	Figure,7B
R5081	T7393	T7391	punct	),7B
R5082	T7394	T7340	punct	.,bound
R5083	T7396	T7397	advmod	Therefore,correlate
R5084	T7398	T7397	punct	", ",correlate
R5085	T7399	T7400	nmod	helicase,activities
R5086	T7400	T7397	nsubj	activities,correlate
R5087	T7401	T7399	cc	and,helicase
R5088	T7402	T7403	compound	DNA,binding
R5089	T7403	T7399	conj	binding,helicase
R5090	T7404	T7397	aux	do,correlate
R5091	T7405	T7397	neg	not,correlate
R5092	T7406	T7397	advmod	necessarily,correlate
R5093	T7407	T7397	prep	with,correlate
R5094	T7408	T7409	det	each,other
R5095	T7409	T7407	pobj	other,with
R5096	T7410	T7397	punct	.,correlate
R5097	T7412	T7413	nsubjpass	This,indicated
R5098	T7414	T7413	auxpass	was,indicated
R5099	T7415	T7413	advmod	also,indicated
R5100	T7416	T7413	agent	by,indicated
R5101	T7417	T7418	poss	our,results
R5102	T7418	T7416	pobj	results,by
R5103	T7419	T7418	amod	previous,results
R5104	T7420	T7421	mark	that,bind
R5105	T7421	T7418	acl	bind,results
R5106	T7422	T7423	nmod	guanine,stretches
R5107	T7423	T7421	nsubj	stretches,bind
R5108	T7424	T7422	cc	and,guanine
R5109	T7425	T7422	conj	cytosine,guanine
R5110	T7426	T7421	aux	can,bind
R5111	T7427	T7421	prep	to,bind
R5112	T7428	T7427	pobj	Mcm,to
R5113	T7429	T7421	cc	but,bind
R5114	T7430	T7431	aux	can,activate
R5115	T7431	T7421	conj	activate,bind
R5116	T7432	T7431	neg	not,activate
R5117	T7433	T7434	det	the,helicase
R5118	T7434	T7431	dobj	helicase,activate
R5119	T7435	T7436	punct	(,13
R5120	T7436	T7431	parataxis	13,activate
R5121	T7437	T7436	punct	),13
R5122	T7438	T7413	punct	.,indicated
R5124	T7722	T7723	compound	DNA,unwinding
R5125	T7723	T7724	nsubjpass	unwinding,inhibited
R5126	T7725	T7723	prep	by,unwinding
R5127	T7726	T7725	pobj	Mcm4,by
R5128	T7727	T7726	punct	/,Mcm4
R5129	T7728	T7726	nummod	6,Mcm4
R5130	T7729	T7726	punct	/,Mcm4
R5131	T7730	T7726	nummod	7,Mcm4
R5132	T7731	T7724	auxpass	is,inhibited
R5133	T7732	T7724	agent	by,inhibited
R5134	T7733	T7734	det	the,sequences
R5135	T7734	T7732	pobj	sequences,by
R5136	T7735	T7736	npadvmod	GC,rich
R5137	T7736	T7734	amod	rich,sequences
R5138	T7737	T7736	punct	-,rich
R5139	T7738	T7734	prep	on,sequences
R5140	T7739	T7740	det	the,segment
R5141	T7740	T7738	pobj	segment,on
R5142	T7741	T7740	compound	duplex,segment
R5143	T7743	T7744	poss	Our,results
R5144	T7744	T7745	nsubj	results,indicate
R5145	T7746	T7747	mark	that,required
R5146	T7747	T7745	ccomp	required,indicate
R5147	T7748	T7749	npadvmod	thymine,rich
R5148	T7749	T7751	amod	rich,DNA
R5149	T7750	T7749	punct	-,rich
R5150	T7751	T7747	nsubjpass	DNA,required
R5151	T7752	T7753	amod	single,stranded
R5152	T7753	T7751	amod	stranded,DNA
R5153	T7754	T7753	punct	-,stranded
R5154	T7755	T7747	auxpass	is,required
R5155	T7756	T7747	prep	for,required
R5156	T7757	T7758	amod	initial,loading
R5157	T7758	T7756	pobj	loading,for
R5158	T7759	T7758	cc	and,loading
R5159	T7760	T7758	conj	activation,loading
R5160	T7761	T7758	prep	of,loading
R5161	T7762	T7763	det	the,helicase
R5162	T7763	T7761	pobj	helicase,of
R5163	T7764	T7763	compound	Mcm,helicase
R5164	T7765	T7745	punct	.,indicate
R5165	T7767	T7768	advmod	However,known
R5166	T7769	T7768	punct	", ",known
R5167	T7770	T7768	nsubjpass	it,known
R5168	T7771	T7768	auxpass	is,known
R5169	T7772	T7768	neg	not,known
R5170	T7773	T7774	mark	whether,required
R5171	T7774	T7768	ccomp	required,known
R5172	T7775	T7776	compound	thymine,sequences
R5173	T7776	T7774	nsubjpass	sequences,required
R5174	T7777	T7774	auxpass	are,required
R5175	T7778	T7774	prep	for,required
R5176	T7779	T7780	amod	processive,unwinding
R5177	T7780	T7778	pobj	unwinding,for
R5178	T7781	T7780	prep	of,unwinding
R5179	T7782	T7783	compound	duplex,DNA
R5180	T7783	T7781	pobj	DNA,of
R5181	T7784	T7768	punct	.,known
R5182	T7786	T7787	advmod	Therefore,examined
R5183	T7788	T7787	punct	", ",examined
R5184	T7789	T7787	nsubj	we,examined
R5185	T7790	T7787	aux	have,examined
R5186	T7791	T7792	mark	whether,affects
R5187	T7792	T7787	ccomp	affects,examined
R5188	T7793	T7794	det	the,composition
R5189	T7794	T7792	nsubj	composition,affects
R5190	T7795	T7794	compound	nucleotide,composition
R5191	T7796	T7794	prep	of,composition
R5192	T7797	T7798	det	the,region
R5193	T7798	T7796	pobj	region,of
R5194	T7799	T7798	compound	duplex,region
R5195	T7800	T7801	poss	its,activity
R5196	T7801	T7792	dobj	activity,affects
R5197	T7802	T7801	compound	unwinding,activity
R5198	T7803	T7787	punct	.,examined
R5199	T7805	T7806	aux	To,address
R5200	T7806	T7807	advcl	address,constructed
R5201	T7808	T7809	det	this,issue
R5202	T7809	T7806	dobj	issue,address
R5203	T7810	T7807	punct	", ",constructed
R5204	T7811	T7807	nsubj	we,constructed
R5205	T7812	T7813	det	a,series
R5206	T7813	T7807	dobj	series,constructed
R5207	T7814	T7813	prep	of,series
R5208	T7815	T7816	npadvmod	T,tailed
R5209	T7816	T7818	amod	tailed,structures
R5210	T7817	T7816	punct	-,tailed
R5211	T7818	T7814	pobj	structures,of
R5212	T7819	T7820	compound	Y,fork
R5213	T7820	T7818	compound	fork,structures
R5214	T7821	T7820	punct	-,fork
R5215	T7822	T7818	punct	(,structures
R5216	T7823	T7824	compound	T,fork
R5217	T7824	T7818	appos	fork,structures
R5218	T7825	T7824	punct	-,fork
R5219	T7826	T7818	punct	),structures
R5220	T7827	T7818	acl	containing,structures
R5221	T7828	T7829	amod	various,sequences
R5222	T7829	T7827	dobj	sequences,containing
R5223	T7830	T7807	prep	in,constructed
R5224	T7831	T7832	det	the,segment
R5225	T7832	T7830	pobj	segment,in
R5226	T7833	T7832	compound	duplex,segment
R5227	T7834	T7807	punct	.,constructed
R5228	T7836	T7837	nsubj	They,carried
R5229	T7838	T7839	amod	varied,contents
R5230	T7839	T7837	dobj	contents,carried
R5231	T7840	T7839	prep	of,contents
R5232	T7841	T7842	compound	cytosine,residues
R5233	T7842	T7840	pobj	residues,of
R5234	T7843	T7837	prep	on,carried
R5235	T7844	T7845	det	the,strand
R5236	T7845	T7843	pobj	strand,on
R5237	T7846	T7847	nummod	3,tail
R5238	T7847	T7845	compound	tail,strand
R5239	T7848	T7846	punct	′,3
R5240	T7849	T7847	punct	-,tail
R5241	T7850	T7837	punct	.,carried
R5242	T7852	T7853	prep	In,bound
R5243	T7854	T7855	compound	gel,shift
R5244	T7855	T7856	compound	shift,assays
R5245	T7856	T7852	pobj	assays,In
R5246	T7857	T7853	punct	", ",bound
R5247	T7858	T7853	nsubj	Mcm4,bound
R5248	T7859	T7858	punct	/,Mcm4
R5249	T7860	T7858	nummod	6,Mcm4
R5250	T7861	T7858	punct	/,Mcm4
R5251	T7862	T7858	nummod	7,Mcm4
R5252	T7863	T7853	prep	to,bound
R5253	T7864	T7865	det	these,substrates
R5254	T7865	T7863	pobj	substrates,to
R5255	T7866	T7867	compound	Y,fork
R5256	T7867	T7865	compound	fork,substrates
R5257	T7868	T7867	punct	-,fork
R5258	T7869	T7853	prep	with,bound
R5259	T7870	T7871	amod	identical,affinity
R5260	T7871	T7869	pobj	affinity,with
R5261	T7872	T7873	punct	(,8A
R5262	T7873	T7871	parataxis	8A,affinity
R5263	T7874	T7873	compound	Figure,8A
R5264	T7875	T7873	punct	),8A
R5265	T7876	T7853	punct	", ",bound
R5266	T7877	T7853	advcl	consistent,bound
R5267	T7878	T7877	prep	with,consistent
R5268	T7879	T7880	det	the,notion
R5269	T7880	T7878	pobj	notion,with
R5270	T7881	T7882	mark	that,binds
R5271	T7882	T7880	acl	binds,notion
R5272	T7883	T7884	det	the,Mcm4
R5273	T7884	T7882	nsubj	Mcm4,binds
R5274	T7885	T7884	punct	/,Mcm4
R5275	T7886	T7884	nummod	6,Mcm4
R5276	T7887	T7884	punct	/,Mcm4
R5277	T7888	T7884	nummod	7,Mcm4
R5278	T7889	T7882	prep	to,binds
R5279	T7890	T7891	amod	single,stranded
R5280	T7891	T7893	amod	stranded,tails
R5281	T7892	T7891	punct	-,stranded
R5282	T7893	T7889	pobj	tails,to
R5283	T7894	T7853	punct	.,bound
R5284	T7896	T7897	advmod	However,displaced
R5285	T7898	T7897	punct	", ",displaced
R5286	T7899	T7897	prep	in,displaced
R5287	T7900	T7901	compound	DNA,helicase
R5288	T7901	T7902	compound	helicase,assays
R5289	T7902	T7899	pobj	assays,in
R5290	T7903	T7897	punct	", ",displaced
R5291	T7904	T7905	compound	T,fork
R5292	T7905	T7897	nsubjpass	fork,displaced
R5293	T7906	T7905	punct	-,fork
R5294	T7907	T7905	punct	/,fork
R5295	T7908	T7909	punct	(,G
R5296	T7909	T7905	appos	G,fork
R5297	T7910	T7909	nmod	C,G
R5298	T7911	T7909	punct	:,G
R5299	T7912	T7909	punct	),G
R5300	T7913	T7909	nummod	49,G
R5301	T7914	T7897	auxpass	was,displaced
R5302	T7915	T7897	advmod	hardly,displaced
R5303	T7916	T7897	agent	by,displaced
R5304	T7917	T7916	pobj	Mcm4,by
R5305	T7918	T7917	punct	/,Mcm4
R5306	T7919	T7917	nummod	6,Mcm4
R5307	T7920	T7917	punct	/,Mcm4
R5308	T7921	T7917	nummod	7,Mcm4
R5309	T7922	T7897	punct	", ",displaced
R5310	T7923	T7897	cc	but,displaced
R5311	T7924	T7925	auxpass	was,displaced
R5312	T7925	T7897	conj	displaced,displaced
R5313	T7926	T7925	advmod	readily,displaced
R5314	T7927	T7925	agent	by,displaced
R5315	T7928	T7929	compound	SV40,helicase
R5316	T7929	T7927	pobj	helicase,by
R5317	T7930	T7931	compound	T,antigen
R5318	T7931	T7929	compound	antigen,helicase
R5319	T7932	T7931	punct	-,antigen
R5320	T7933	T7929	compound	DNA,helicase
R5321	T7934	T7935	punct	(,8B
R5322	T7935	T7925	parataxis	8B,displaced
R5323	T7936	T7935	compound	Figure,8B
R5324	T7937	T7935	punct	),8B
R5325	T7938	T7897	punct	.,displaced
R5326	T7940	T7941	advmod	When,inserted
R5327	T7941	T7946	advcl	inserted,increased
R5328	T7942	T7941	nsubjpass	thymine,inserted
R5329	T7943	T7942	cc	or,thymine
R5330	T7944	T7942	conj	adenine,thymine
R5331	T7945	T7941	auxpass	is,inserted
R5332	T7947	T7941	prep	as,inserted
R5333	T7948	T7949	det	every,nucleotide
R5334	T7949	T7947	pobj	nucleotide,as
R5335	T7950	T7949	amod	third,nucleotide
R5336	T7951	T7952	punct	(,repeats
R5337	T7952	T7949	parataxis	repeats,nucleotide
R5338	T7953	T7952	prep	of,repeats
R5339	T7954	T7953	pobj	CCT,of
R5340	T7955	T7954	cc	or,CCT
R5341	T7956	T7954	conj	CCA,CCT
R5342	T7957	T7952	punct	),repeats
R5343	T7958	T7946	punct	", ",increased
R5344	T7959	T7960	det	the,extent
R5345	T7960	T7946	nsubj	extent,increased
R5346	T7961	T7960	prep	of,extent
R5347	T7962	T7961	pobj	unwinding,of
R5348	T7963	T7964	punct	(,GGA
R5349	T7964	T7946	dobj	GGA,increased
R5350	T7965	T7966	nmod	T,fork
R5351	T7966	T7964	nmod	fork,GGA
R5352	T7967	T7966	punct	-,fork
R5353	T7968	T7964	punct	/,GGA
R5354	T7969	T7964	punct	(,GGA
R5355	T7970	T7964	nmod	CCT,GGA
R5356	T7971	T7964	punct	:,GGA
R5357	T7972	T7964	punct	),GGA
R5358	T7973	T7964	nummod	16,GGA
R5359	T7974	T7964	cc	and,GGA
R5360	T7975	T7976	nmod	T,fork
R5361	T7976	T7978	nmod	fork,GGT
R5362	T7977	T7976	punct	-,fork
R5363	T7978	T7964	conj	GGT,GGA
R5364	T7979	T7978	punct	/,GGT
R5365	T7980	T7978	punct	(,GGT
R5366	T7981	T7978	nmod	CCA,GGT
R5367	T7982	T7978	punct	:,GGT
R5368	T7983	T7978	punct	),GGT
R5369	T7984	T7978	nummod	16,GGT
R5370	T7985	T7946	punct	),increased
R5371	T7986	T7946	punct	.,increased
R5372	T7988	T7989	det	The,efficiency
R5373	T7989	T7990	nsubjpass	efficiency,correlated
R5374	T7991	T7989	prep	of,efficiency
R5375	T7992	T7991	pobj	unwinding,of
R5376	T7993	T7990	auxpass	is,correlated
R5377	T7994	T7990	advmod	roughly,correlated
R5378	T7995	T7990	prep	with,correlated
R5379	T7996	T7997	det	the,content
R5380	T7997	T7995	pobj	content,with
R5381	T7998	T7997	prep	of,content
R5382	T7999	T8000	compound	GC,pairs
R5383	T8000	T7998	pobj	pairs,of
R5384	T8001	T8000	prep	in,pairs
R5385	T8002	T8003	det	the,segment
R5386	T8003	T8001	pobj	segment,in
R5387	T8004	T8003	compound	duplex,segment
R5388	T8005	T8006	punct	[,GGTT
R5389	T8006	T8000	appos	GGTT,pairs
R5390	T8007	T8008	nmod	T,fork
R5391	T8008	T8006	nmod	fork,GGTT
R5392	T8009	T8008	punct	-,fork
R5393	T8010	T8006	punct	/,GGTT
R5394	T8011	T8006	punct	(,GGTT
R5395	T8012	T8006	nmod	CCAA,GGTT
R5396	T8013	T8006	punct	:,GGTT
R5397	T8014	T8006	punct	),GGTT
R5398	T8015	T8006	nummod	12,GGTT
R5399	T8016	T8006	punct	", ",GGTT
R5400	T8017	T8018	nmod	T,fork
R5401	T8018	T8020	nmod	fork,GGAA
R5402	T8019	T8018	punct	-,fork
R5403	T8020	T8006	conj	GGAA,GGTT
R5404	T8021	T8020	punct	/,GGAA
R5405	T8022	T8020	punct	(,GGAA
R5406	T8023	T8020	nmod	CCTT,GGAA
R5407	T8024	T8020	punct	:,GGAA
R5408	T8025	T8020	punct	),GGAA
R5409	T8026	T8020	nummod	12,GGAA
R5410	T8027	T8020	punct	", ",GGAA
R5411	T8028	T8029	nmod	T,fork
R5412	T8029	T8031	nmod	fork,GTTT
R5413	T8030	T8029	punct	-,fork
R5414	T8031	T8020	conj	GTTT,GGAA
R5415	T8032	T8031	punct	/,GTTT
R5416	T8033	T8031	punct	(,GTTT
R5417	T8034	T8031	nmod	CAAA,GTTT
R5418	T8035	T8031	punct	:,GTTT
R5419	T8036	T8031	punct	),GTTT
R5420	T8037	T8031	nummod	12,GTTT
R5421	T8038	T8031	cc	and,GTTT
R5422	T8039	T8040	nmod	T,fork
R5423	T8040	T8042	nmod	fork,GAAA
R5424	T8041	T8040	punct	-,fork
R5425	T8042	T8031	conj	GAAA,GTTT
R5426	T8043	T8042	punct	/,GAAA
R5427	T8044	T8042	punct	(,GAAA
R5428	T8045	T8042	nmod	CTTT,GAAA
R5429	T8046	T8042	punct	:,GAAA
R5430	T8047	T8042	punct	),GAAA
R5431	T8048	T8042	nummod	12,GAAA
R5432	T8049	T8050	punct	;,8B
R5433	T8050	T7990	parataxis	8B,correlated
R5434	T8051	T8050	compound	Figure,8B
R5435	T8052	T8050	punct	],8B
R5436	T8053	T7990	punct	.,correlated
R5437	T8055	T8056	nsubj	It,appears
R5438	T8057	T8058	mark	that,unwound
R5439	T8058	T8056	ccomp	unwound,appears
R5440	T8059	T8060	det	the,segment
R5441	T8060	T8058	nsubjpass	segment,unwound
R5442	T8061	T8060	compound	duplex,segment
R5443	T8062	T8060	acl	containing,segment
R5444	T8063	T8064	punct	<,50
R5445	T8064	T8065	nummod	50,%
R5446	T8065	T8066	compound	%,pairs
R5447	T8066	T8062	dobj	pairs,containing
R5448	T8067	T8066	compound	GC,pairs
R5449	T8068	T8058	auxpass	is,unwound
R5450	T8069	T8058	advmod	efficiently,unwound
R5451	T8070	T8071	punct	(,8C
R5452	T8071	T8058	parataxis	8C,unwound
R5453	T8072	T8071	compound	Figure,8C
R5454	T8073	T8071	punct	),8C
R5455	T8074	T8056	punct	.,appears
R5456	T8076	T8077	nsubj	We,examined
R5457	T8078	T8077	advmod	next,examined
R5458	T8079	T8080	det	the,effect
R5459	T8080	T8077	dobj	effect,examined
R5460	T8081	T8080	prep	of,effect
R5461	T8082	T8083	npadvmod	GC,rich
R5462	T8083	T8085	amod	rich,segments
R5463	T8084	T8083	punct	-,rich
R5464	T8085	T8081	pobj	segments,of
R5465	T8086	T8080	prep	on,effect
R5466	T8087	T8088	det	a,DNA
R5467	T8088	T8086	pobj	DNA,on
R5468	T8089	T8088	amod	partial,DNA
R5469	T8090	T8088	compound	heteroduplex,DNA
R5470	T8091	T8088	prep	on,DNA
R5471	T8092	T8093	det	a,DNA
R5472	T8093	T8091	pobj	DNA,on
R5473	T8094	T8095	amod	single,stranded
R5474	T8095	T8093	amod	stranded,DNA
R5475	T8096	T8095	punct	-,stranded
R5476	T8097	T8093	amod	circular,DNA
R5477	T8098	T8077	punct	.,examined
R5478	T8100	T8101	nsubj	We,constructed
R5479	T8102	T8101	aux	have,constructed
R5480	T8103	T8104	nummod	two,sets
R5481	T8104	T8101	dobj	sets,constructed
R5482	T8105	T8104	prep	of,sets
R5483	T8106	T8107	npadvmod	5,tailed
R5484	T8107	T8110	amod	tailed,substrates
R5485	T8108	T8106	punct	′,5
R5486	T8109	T8107	punct	-,tailed
R5487	T8110	T8105	pobj	substrates,of
R5488	T8111	T8112	amod	partial,heteroduplex
R5489	T8112	T8110	compound	heteroduplex,substrates
R5490	T8113	T8110	compound	DNA,substrates
R5491	T8114	T8110	acl	containing,substrates
R5492	T8115	T8116	compound	duplex,regions
R5493	T8116	T8114	dobj	regions,containing
R5494	T8117	T8116	prep	of,regions
R5495	T8118	T8119	amod	variable,lengths
R5496	T8119	T8117	pobj	lengths,of
R5497	T8120	T8104	punct	;,sets
R5498	T8121	T8104	appos	one,sets
R5499	T8122	T8121	prep	on,one
R5500	T8123	T8124	compound	M13mp18,vector
R5501	T8124	T8122	pobj	vector,on
R5502	T8125	T8121	cc	and,one
R5503	T8126	T8127	det	the,other
R5504	T8127	T8121	conj	other,one
R5505	T8128	T8127	prep	on,other
R5506	T8129	T8128	pobj	M13mp19,on
R5507	T8130	T8121	acl	containing,one
R5508	T8131	T8132	det	a,segment
R5509	T8132	T8130	dobj	segment,containing
R5510	T8133	T8134	nummod	66,nt
R5511	T8134	T8135	npadvmod	nt,long
R5512	T8135	T8132	amod	long,segment
R5513	T8136	T8137	npadvmod	G,rich
R5514	T8137	T8132	amod	rich,segment
R5515	T8138	T8137	punct	-,rich
R5516	T8139	T8132	advmod	downstream,segment
R5517	T8140	T8139	prep	of,downstream
R5518	T8141	T8142	det	the,oligonucleotide
R5519	T8142	T8140	pobj	oligonucleotide,of
R5520	T8143	T8142	amod	hybridizing,oligonucleotide
R5521	T8144	T8101	punct	.,constructed
R5522	T8146	T8147	nsubj	We,found
R5523	T8148	T8149	mark	that,displace
R5524	T8149	T8147	ccomp	displace,found
R5525	T8150	T8151	det	the,helicase
R5526	T8151	T8149	nsubj	helicase,displace
R5527	T8152	T8151	nmod	Mcm4,helicase
R5528	T8153	T8152	punct	/,Mcm4
R5529	T8154	T8152	nummod	6,Mcm4
R5530	T8155	T8152	punct	/,Mcm4
R5531	T8156	T8152	nummod	7,Mcm4
R5532	T8157	T8149	aux	can,displace
R5533	T8158	T8159	compound	duplex,DNA
R5534	T8159	T8149	dobj	DNA,displace
R5535	T8160	T8161	quantmod	up,350
R5536	T8161	T8163	nummod	350,nt
R5537	T8162	T8161	quantmod	to,350
R5538	T8163	T8164	npadvmod	nt,long
R5539	T8164	T8159	amod	long,DNA
R5540	T8165	T8149	prep	on,displace
R5541	T8166	T8167	poss	its,own
R5542	T8167	T8165	pobj	own,on
R5543	T8168	T8149	prep	on,displace
R5544	T8169	T8170	det	the,substrate
R5545	T8170	T8168	pobj	substrate,on
R5546	T8171	T8170	amod	former,substrate
R5547	T8172	T8173	punct	(,8D
R5548	T8173	T8149	parataxis	8D,displace
R5549	T8174	T8173	compound	Figure,8D
R5550	T8175	T8173	punct	", ",8D
R5551	T8176	T8173	amod	left,8D
R5552	T8177	T8173	punct	),8D
R5553	T8178	T8147	punct	", ",found
R5554	T8179	T8147	advcl	consistent,found
R5555	T8180	T8179	prep	with,consistent
R5556	T8181	T8182	amod	previous,results
R5557	T8182	T8180	pobj	results,with
R5558	T8183	T8184	punct	(,13
R5559	T8184	T8182	parataxis	13,results
R5560	T8185	T8184	punct	),13
R5561	T8186	T8147	punct	.,found
R5562	T8188	T8189	prep	In,inhibited
R5563	T8190	T8188	pobj	contrast,In
R5564	T8191	T8189	punct	", ",inhibited
R5565	T8192	T8189	nsubjpass	displacement,inhibited
R5566	T8193	T8189	auxpass	was,inhibited
R5567	T8194	T8189	prep	over,inhibited
R5568	T8195	T8196	det	the,segments
R5569	T8196	T8194	pobj	segments,over
R5570	T8197	T8198	npadvmod	GC,rich
R5571	T8198	T8196	amod	rich,segments
R5572	T8199	T8198	punct	-,rich
R5573	T8200	T8196	prep	on,segments
R5574	T8201	T8202	det	the,substrate
R5575	T8202	T8200	pobj	substrate,on
R5576	T8203	T8202	amod	latter,substrate
R5577	T8204	T8205	punct	(,panel
R5578	T8205	T8196	parataxis	panel,segments
R5579	T8206	T8207	compound	Figure,8D
R5580	T8207	T8205	dep	8D,panel
R5581	T8208	T8205	punct	", ",panel
R5582	T8209	T8205	amod	right,panel
R5583	T8210	T8205	punct	),panel
R5584	T8211	T8189	punct	", ",inhibited
R5585	T8212	T8213	mark	although,observed
R5586	T8213	T8189	advcl	observed,inhibited
R5587	T8214	T8213	nsubjpass	displacement,observed
R5588	T8215	T8216	quantmod	up,250
R5589	T8216	T8218	nummod	250,nt
R5590	T8217	T8216	quantmod	to,250
R5591	T8218	T8214	appos	nt,displacement
R5592	T8219	T8218	prep	in,nt
R5593	T8220	T8219	pobj	length,in
R5594	T8221	T8218	punct	", ",nt
R5595	T8222	T8223	dep	albeit,at
R5596	T8223	T8218	prep	at,nt
R5597	T8224	T8225	det	a,level
R5598	T8225	T8223	pobj	level,at
R5599	T8226	T8225	amod	reduced,level
R5600	T8227	T8213	punct	", ",observed
R5601	T8228	T8213	auxpass	was,observed
R5602	T8229	T8213	prep	at,observed
R5603	T8230	T8231	det	a,concentration
R5604	T8231	T8229	pobj	concentration,at
R5605	T8232	T8231	amod	high,concentration
R5606	T8233	T8231	prep	of,concentration
R5607	T8234	T8235	nmod	Mcm4,complex
R5608	T8235	T8233	pobj	complex,of
R5609	T8236	T8234	punct	/,Mcm4
R5610	T8237	T8234	nummod	6,Mcm4
R5611	T8238	T8234	punct	/,Mcm4
R5612	T8239	T8234	nummod	7,Mcm4
R5613	T8240	T8189	punct	.,inhibited
R5614	T8242	T8243	det	This,result
R5615	T8243	T8244	nsubj	result,is
R5616	T8245	T8244	acomp	consistent,is
R5617	T8246	T8245	prep	with,consistent
R5618	T8247	T8246	pobj	that,with
R5619	T8248	T8247	prep	on,that
R5620	T8249	T8250	compound	Y,fork
R5621	T8250	T8248	pobj	fork,on
R5622	T8251	T8250	punct	-,fork
R5623	T8252	T8244	cc	and,is
R5624	T8253	T8244	conj	indicates,is
R5625	T8254	T8255	mark	that,is
R5626	T8255	T8253	ccomp	is,indicates
R5627	T8256	T8257	npadvmod	GC,rich
R5628	T8257	T8259	amod	rich,segment
R5629	T8258	T8257	punct	-,rich
R5630	T8259	T8255	nsubj	segment,is
R5631	T8260	T8259	compound	duplex,segment
R5632	T8261	T8255	acomp	inhibitory,is
R5633	T8262	T8261	prep	for,inhibitory
R5634	T8263	T8262	pobj	unwinding,for
R5635	T8264	T8263	prep	by,unwinding
R5636	T8265	T8266	det	the,helicase
R5637	T8266	T8264	pobj	helicase,by
R5638	T8267	T8266	compound	Mcm,helicase
R5639	T8268	T8244	punct	.,is
R5640	T8270	T8271	det	These,results
R5641	T8271	T8272	nsubj	results,indicate
R5642	T8273	T8272	aux	may,indicate
R5643	T8274	T8275	mark	that,facilitate
R5644	T8275	T8272	ccomp	facilitate,indicate
R5645	T8276	T8277	det	the,presence
R5646	T8277	T8275	nsubj	presence,facilitate
R5647	T8278	T8277	prep	of,presence
R5648	T8279	T8280	compound	thymine,adenine
R5649	T8280	T8282	compound	adenine,pairs
R5650	T8281	T8280	punct	–,adenine
R5651	T8282	T8278	pobj	pairs,of
R5652	T8283	T8282	prep	with,pairs
R5653	T8284	T8285	det	a,frequency
R5654	T8285	T8283	pobj	frequency,with
R5655	T8286	T8285	amod	certain,frequency
R5656	T8287	T8277	prep	in,presence
R5657	T8288	T8289	det	the,region
R5658	T8289	T8287	pobj	region,in
R5659	T8290	T8289	compound	duplex,region
R5660	T8291	T8275	aux	may,facilitate
R5661	T8292	T8293	amod	continuous,unwinding
R5662	T8293	T8275	dobj	unwinding,facilitate
R5663	T8294	T8293	prep	by,unwinding
R5664	T8295	T8294	pobj	Mcm4,by
R5665	T8296	T8295	punct	/,Mcm4
R5666	T8297	T8295	nummod	6,Mcm4
R5667	T8298	T8295	punct	/,Mcm4
R5668	T8299	T8295	nummod	7,Mcm4
R5669	T8300	T8272	punct	.,indicate
R5670	T8302	T8303	advmod	Alternatively,is
R5671	T8304	T8303	punct	", ",is
R5672	T8305	T8303	nsubj	Mcm4,is
R5673	T8306	T8305	punct	/,Mcm4
R5674	T8307	T8305	nummod	6,Mcm4
R5675	T8308	T8305	punct	/,Mcm4
R5676	T8309	T8305	nummod	7,Mcm4
R5677	T8310	T8303	neg	not,is
R5678	T8311	T8303	acomp	efficient,is
R5679	T8312	T8311	advmod	enough,efficient
R5680	T8313	T8314	aux	to,displace
R5681	T8314	T8311	xcomp	displace,efficient
R5682	T8315	T8316	advmod	thermodynamically,stable
R5683	T8316	T8317	amod	stable,segment
R5684	T8317	T8314	dobj	segment,displace
R5685	T8318	T8319	npadvmod	GC,paired
R5686	T8319	T8317	amod	paired,segment
R5687	T8320	T8319	punct	-,paired
R5688	T8321	T8319	npadvmod	base,paired
R5689	T8322	T8319	punct	-,paired
R5690	T8323	T8303	punct	.,is
R5691	T8325	T8326	prep	In,designed
R5692	T8327	T8325	pobj	order,In
R5693	T8328	T8329	aux	to,distinguish
R5694	T8329	T8327	acl	distinguish,order
R5695	T8330	T8331	det	these,possibilities
R5696	T8331	T8329	dobj	possibilities,distinguish
R5697	T8332	T8331	nummod	two,possibilities
R5698	T8333	T8326	punct	", ",designed
R5699	T8334	T8326	nsubj	we,designed
R5700	T8335	T8336	det	a,substrate
R5701	T8336	T8326	dobj	substrate,designed
R5702	T8337	T8336	amod	new,substrate
R5703	T8338	T8339	compound	T,fork
R5704	T8339	T8336	compound	fork,substrate
R5705	T8340	T8339	punct	-,fork
R5706	T8341	T8336	acl	containing,substrate
R5707	T8342	T8343	nmod	inosine,residue
R5708	T8343	T8341	dobj	residue,containing
R5709	T8344	T8342	punct	(,inosine
R5710	T8345	T8342	appos	I,inosine
R5711	T8346	T8343	punct	),residue
R5712	T8347	T8348	advmod	instead,of
R5713	T8348	T8343	prep	of,residue
R5714	T8349	T8350	compound	guanosine,residue
R5715	T8350	T8348	pobj	residue,of
R5716	T8351	T8326	punct	.,designed
R5717	T8353	T8354	det	The,stability
R5718	T8354	T8356	nsubj	stability,is
R5719	T8355	T8354	amod	thermal,stability
R5720	T8357	T8354	prep	of,stability
R5721	T8358	T8359	det	the,pair
R5722	T8359	T8357	pobj	pair,of
R5723	T8360	T8361	nmod	I,C
R5724	T8361	T8359	compound	C,pair
R5725	T8362	T8361	punct	:,C
R5726	T8363	T8359	compound	base,pair
R5727	T8364	T8356	acomp	lower,is
R5728	T8365	T8364	prep	than,lower
R5729	T8366	T8365	pobj	that,than
R5730	T8367	T8366	prep	of,that
R5731	T8368	T8369	det	the,pair
R5732	T8369	T8367	pobj	pair,of
R5733	T8370	T8371	nmod	G,C
R5734	T8371	T8369	compound	C,pair
R5735	T8372	T8371	punct	:,C
R5736	T8373	T8369	compound	base,pair
R5737	T8374	T8356	prep	in,is
R5738	T8375	T8376	compound	duplex,DNA
R5739	T8376	T8374	pobj	DNA,in
R5740	T8377	T8356	prep	due,is
R5741	T8378	T8377	pcomp	to,due
R5742	T8379	T8377	pobj	loss,due
R5743	T8380	T8379	prep	of,loss
R5744	T8381	T8382	nummod	one,bond
R5745	T8382	T8380	pobj	bond,of
R5746	T8383	T8382	compound	hydrogen,bond
R5747	T8384	T8356	cc	and,is
R5748	T8385	T8356	conj	is,is
R5749	T8386	T8387	advmod	even,lower
R5750	T8387	T8385	acomp	lower,is
R5751	T8388	T8387	prep	than,lower
R5752	T8389	T8390	nmod	A,T
R5753	T8390	T8392	compound	T,pair
R5754	T8391	T8390	punct	:,T
R5755	T8392	T8388	pobj	pair,than
R5756	T8393	T8392	compound	base,pair
R5757	T8394	T8395	punct	(,31
R5758	T8395	T8392	parataxis	31,pair
R5759	T8396	T8395	punct	),31
R5760	T8397	T8356	punct	.,is
R5761	T8399	T8400	nsubj	We,constructed
R5762	T8401	T8402	det	the,substrates
R5763	T8402	T8400	dobj	substrates,constructed
R5764	T8403	T8404	npadvmod	T,tailed
R5765	T8404	T8402	amod	tailed,substrates
R5766	T8405	T8404	punct	-,tailed
R5767	T8406	T8402	compound	fork,substrates
R5768	T8407	T8402	acl	containing,substrates
R5769	T8408	T8409	det	the,segment
R5770	T8409	T8407	dobj	segment,containing
R5771	T8410	T8411	punct	(,CGG
R5772	T8411	T8409	nmod	CGG,segment
R5773	T8412	T8411	nmod	GCC,CGG
R5774	T8413	T8411	punct	:,CGG
R5775	T8414	T8411	punct	),CGG
R5776	T8415	T8411	nummod	10,CGG
R5777	T8416	T8411	punct	", ",CGG
R5778	T8417	T8418	punct	(,CIG
R5779	T8418	T8411	conj	CIG,CGG
R5780	T8419	T8418	nmod	GCC,CIG
R5781	T8420	T8418	punct	:,CIG
R5782	T8421	T8418	punct	),CIG
R5783	T8422	T8418	nummod	10,CIG
R5784	T8423	T8418	cc	or,CIG
R5785	T8424	T8425	punct	(,CTT
R5786	T8425	T8418	conj	CTT,CIG
R5787	T8426	T8425	nmod	GAA,CTT
R5788	T8427	T8425	punct	:,CTT
R5789	T8428	T8425	punct	),CTT
R5790	T8429	T8425	nummod	10,CTT
R5791	T8430	T8409	compound	duplex,segment
R5792	T8431	T8409	compound	DNA,segment
R5793	T8432	T8433	punct	(,8E
R5794	T8433	T8407	parataxis	8E,containing
R5795	T8434	T8433	compound	Figure,8E
R5796	T8435	T8433	punct	),8E
R5797	T8436	T8400	punct	.,constructed
R5798	T8438	T8439	nsubj	Mcm4,displaced
R5799	T8440	T8438	punct	/,Mcm4
R5800	T8441	T8438	nummod	6,Mcm4
R5801	T8442	T8438	punct	/,Mcm4
R5802	T8443	T8438	nummod	7,Mcm4
R5803	T8444	T8439	advmod	hardly,displaced
R5804	T8445	T8446	det	the,duplex
R5805	T8446	T8439	dobj	duplex,displaced
R5806	T8447	T8448	nummod	31,nt
R5807	T8448	T8449	npadvmod	nt,long
R5808	T8449	T8446	amod	long,duplex
R5809	T8450	T8446	prep	of,duplex
R5810	T8451	T8452	nmod	GCC,CGG
R5811	T8452	T8454	compound	CGG,repeats
R5812	T8453	T8452	punct	:,CGG
R5813	T8454	T8450	pobj	repeats,of
R5814	T8455	T8439	punct	", ",displaced
R5815	T8456	T8457	mark	whereas,displaced
R5816	T8457	T8439	advcl	displaced,displaced
R5817	T8458	T8459	det	the,CTT
R5818	T8459	T8457	nsubjpass	CTT,displaced
R5819	T8460	T8461	nmod	T,fork
R5820	T8461	T8459	nmod	fork,CTT
R5821	T8462	T8461	punct	-,fork
R5822	T8463	T8459	punct	/,CTT
R5823	T8464	T8459	punct	(,CTT
R5824	T8465	T8459	nmod	GAA,CTT
R5825	T8466	T8459	punct	:,CTT
R5826	T8467	T8459	punct	),CTT
R5827	T8468	T8459	nummod	10,CTT
R5828	T8469	T8457	auxpass	was,displaced
R5829	T8470	T8457	advmod	efficiently,displaced
R5830	T8471	T8457	punct	", ",displaced
R5831	T8472	T8473	mark	as,described
R5832	T8473	T8457	advcl	described,displaced
R5833	T8474	T8473	advmod	above,described
R5834	T8475	T8439	punct	.,displaced
R5835	T8477	T8478	nmod	T,fork
R5836	T8478	T8480	nmod	fork,CIG
R5837	T8479	T8478	punct	-,fork
R5838	T8480	T8485	nsubjpass	CIG,displaced
R5839	T8481	T8480	punct	/,CIG
R5840	T8482	T8480	punct	(,CIG
R5841	T8483	T8480	nmod	GCC,CIG
R5842	T8484	T8480	punct	:,CIG
R5843	T8486	T8480	punct	),CIG
R5844	T8487	T8480	nummod	10,CIG
R5845	T8488	T8485	auxpass	was,displaced
R5846	T8489	T8485	prep	with,displaced
R5847	T8490	T8489	pobj	efficiency,with
R5848	T8491	T8492	advmod	much,greater
R5849	T8492	T8490	amod	greater,efficiency
R5850	T8493	T8492	prep	than,greater
R5851	T8494	T8493	pobj	that,than
R5852	T8495	T8494	prep	of,that
R5853	T8496	T8497	nmod	T,fork
R5854	T8497	T8499	nmod	fork,CGG
R5855	T8498	T8497	punct	-,fork
R5856	T8499	T8495	pobj	CGG,of
R5857	T8500	T8497	punct	/,fork
R5858	T8501	T8499	punct	(,CGG
R5859	T8502	T8499	nmod	GCC,CGG
R5860	T8503	T8499	punct	:,CGG
R5861	T8504	T8499	punct	),CGG
R5862	T8505	T8499	nummod	10,CGG
R5863	T8506	T8485	punct	", ",displaced
R5864	T8507	T8485	advcl	indicating,displaced
R5865	T8508	T8509	mark	that,is
R5866	T8509	T8507	ccomp	is,indicating
R5867	T8510	T8511	det	the,thermostability
R5868	T8511	T8509	nsubj	thermostability,is
R5869	T8512	T8511	prep	of,thermostability
R5870	T8513	T8514	det	the,segment
R5871	T8514	T8512	pobj	segment,of
R5872	T8515	T8514	compound	duplex,segment
R5873	T8516	T8511	punct	", ",thermostability
R5874	T8517	T8511	cc	not,thermostability
R5875	T8518	T8519	det	the,lack
R5876	T8519	T8511	conj	lack,thermostability
R5877	T8520	T8519	prep	of,lack
R5878	T8521	T8522	compound	AT,pair
R5879	T8522	T8520	pobj	pair,of
R5880	T8523	T8522	compound	base,pair
R5881	T8524	T8509	punct	", ",is
R5882	T8525	T8509	acomp	responsible,is
R5883	T8526	T8525	prep	for,responsible
R5884	T8527	T8526	pobj	inability,for
R5885	T8528	T8527	prep	of,inability
R5886	T8529	T8528	pobj	Mcm4,of
R5887	T8530	T8529	punct	/,Mcm4
R5888	T8531	T8529	nummod	6,Mcm4
R5889	T8532	T8529	punct	/,Mcm4
R5890	T8533	T8529	nummod	7,Mcm4
R5891	T8534	T8535	aux	to,displace
R5892	T8535	T8527	acl	displace,inability
R5893	T8536	T8537	det	the,duplex
R5894	T8537	T8535	dobj	duplex,displace
R5895	T8538	T8485	punct	.,displaced

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PMC:1140370 / 10895-28058 JSON TXT 5 Projects