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PMC:1087847 / 7141-8535 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T12193 10-17 NN denotes Annexin
T12194 18-20 NN denotes A7
T12195 21-37 NN denotes immunoreactivity
T12196 38-40 IN denotes in
T12197 41-46 JJ denotes early
T12199 47-52 NN denotes mouse
T12198 53-60 NNS denotes embryos
T12200 60-61 . denotes .
T12201 61-212 sentence denotes (A) Phase contrast, embryo E5: The egg cylinder consists of an inner cell mass (a) representing the ectoderm and an outer layer of endoderm cells (b).
T12202 62-63 -LRB- denotes (
T12203 63-64 LS denotes A
T12205 64-65 -RRB- denotes )
T12206 66-71 NN denotes Phase
T12207 72-80 NN denotes contrast
T12208 80-82 , denotes ,
T12209 82-88 NN denotes embryo
T12210 89-91 NN denotes E5
T12211 91-93 : denotes :
T12212 93-96 DT denotes The
T12214 97-100 NN denotes egg
T12213 101-109 NN denotes cylinder
T12204 110-118 VBZ denotes consists
T12215 119-121 IN denotes of
T12216 122-124 DT denotes an
T12218 125-130 JJ denotes inner
T12219 131-135 NN denotes cell
T12217 136-140 NN denotes mass
T12220 141-142 -LRB- denotes (
T12221 142-143 NN denotes a
T12222 143-144 -RRB- denotes )
T12223 145-157 VBG denotes representing
T12224 158-161 DT denotes the
T12225 162-170 NN denotes ectoderm
T12226 171-174 CC denotes and
T12227 175-177 DT denotes an
T12229 178-183 JJ denotes outer
T12228 184-189 NN denotes layer
T12230 190-192 IN denotes of
T12231 193-201 NN denotes endoderm
T12232 202-207 NNS denotes cells
T12233 208-209 -LRB- denotes (
T12234 209-210 NN denotes b
T12235 210-211 -RRB- denotes )
T12236 211-212 . denotes .
T12237 212-331 sentence denotes (B) Immunostaining of the paraffin section was performed using purified mAb 203–217 and Cy3-conjugated anti-mouse IgG.
T12238 213-214 -LRB- denotes (
T12239 214-215 LS denotes B
T12241 215-216 -RRB- denotes )
T12242 217-231 NN denotes Immunostaining
T12243 232-234 IN denotes of
T12244 235-238 DT denotes the
T12246 239-247 NN denotes paraffin
T12245 248-255 NN denotes section
T12247 256-259 VBD denotes was
T12240 260-269 VBN denotes performed
T12248 270-275 VBG denotes using
T12249 276-284 VBN denotes purified
T12250 285-288 NN denotes mAb
T12251 289-292 CD denotes 203
T12252 292-293 SYM denotes –
T12253 293-296 CD denotes 217
T12254 297-300 CC denotes and
T12255 301-304 NN denotes Cy3
T12257 304-305 HYPH denotes -
T12256 305-315 VBN denotes conjugated
T12259 316-326 JJ denotes anti-mouse
T12258 327-330 NN denotes IgG
T12260 330-331 . denotes .
T12261 331-472 sentence denotes Annexin A7 is expressed in both cell types of the egg cylinder with a strong staining of the endoderm and a weaker staining of the ectoderm.
T12262 332-339 NN denotes Annexin
T12263 340-342 NN denotes A7
T12265 343-345 VBZ denotes is
T12264 346-355 VBN denotes expressed
T12266 356-358 IN denotes in
T12267 359-363 DT denotes both
T12269 364-368 NN denotes cell
T12268 369-374 NNS denotes types
T12270 375-377 IN denotes of
T12271 378-381 DT denotes the
T12273 382-385 NN denotes egg
T12272 386-394 NN denotes cylinder
T12274 395-399 IN denotes with
T12275 400-401 DT denotes a
T12277 402-408 JJ denotes strong
T12276 409-417 NN denotes staining
T12278 418-420 IN denotes of
T12279 421-424 DT denotes the
T12280 425-433 NN denotes endoderm
T12281 434-437 CC denotes and
T12282 438-439 DT denotes a
T12284 440-446 JJR denotes weaker
T12283 447-455 NN denotes staining
T12285 456-458 IN denotes of
T12286 459-462 DT denotes the
T12287 463-471 NN denotes ectoderm
T12288 471-472 . denotes .
T12289 472-515 sentence denotes The nuclei are devoid of immune reactions.
T12290 473-476 DT denotes The
T12291 477-483 NNS denotes nuclei
T12292 484-487 VBP denotes are
T12293 488-494 JJ denotes devoid
T12294 495-497 IN denotes of
T12295 498-504 JJ denotes immune
T12296 505-514 NNS denotes reactions
T12297 514-515 . denotes .
T12298 515-575 sentence denotes (C) Negative control using the secondary Cy3-antibody only.
T12299 516-517 -LRB- denotes (
T12301 517-518 LS denotes C
T12302 518-519 -RRB- denotes )
T12303 520-528 JJ denotes Negative
T12300 529-536 NN denotes control
T12304 537-542 VBG denotes using
T12305 543-546 DT denotes the
T12307 547-556 JJ denotes secondary
T12308 557-560 NN denotes Cy3
T12309 560-561 HYPH denotes -
T12306 561-569 NN denotes antibody
T12310 570-574 RB denotes only
T12311 574-575 . denotes .
T12312 575-696 sentence denotes (D-F) Annexin A7 expression in the proximal neural tube (D) and nearby neural fold (E,F), embryo E8, transverse section.
T12313 576-577 -LRB- denotes (
T12314 577-578 LS denotes D
T12316 578-579 SYM denotes -
T12317 579-580 LS denotes F
T12318 580-581 -RRB- denotes )
T12319 582-589 NN denotes Annexin
T12320 590-592 NN denotes A7
T12321 593-603 NN denotes expression
T12322 604-606 IN denotes in
T12323 607-610 DT denotes the
T12325 611-619 JJ denotes proximal
T12326 620-626 JJ denotes neural
T12324 627-631 NN denotes tube
T12327 632-633 -LRB- denotes (
T12328 633-634 NN denotes D
T12329 634-635 -RRB- denotes )
T12330 636-639 CC denotes and
T12331 640-646 JJ denotes nearby
T12333 647-653 JJ denotes neural
T12332 654-658 NN denotes fold
T12334 659-660 -LRB- denotes (
T12336 660-661 NN denotes E
T12337 661-662 , denotes ,
T12335 662-663 NN denotes F
T12338 663-664 -RRB- denotes )
T12339 664-666 , denotes ,
T12340 666-672 NN denotes embryo
T12341 673-675 NN denotes E8
T12342 675-677 , denotes ,
T12343 677-687 JJ denotes transverse
T12315 688-695 NN denotes section
T12344 695-696 . denotes .
T12345 696-838 sentence denotes Immunolabeling of Annexin A7 was performed with purified mAb 203–217 and visualization was with an Alexa Fluor 488-conjugated anti-mouse IgG.
T12346 697-711 NN denotes Immunolabeling
T12348 712-714 IN denotes of
T12349 715-722 NN denotes Annexin
T12350 723-725 NN denotes A7
T12351 726-729 VBD denotes was
T12347 730-739 VBN denotes performed
T12352 740-744 IN denotes with
T12353 745-753 VBN denotes purified
T12355 754-757 NN denotes mAb
T12354 758-761 CD denotes 203
T12356 761-762 SYM denotes –
T12357 762-765 CD denotes 217
T12358 766-769 CC denotes and
T12359 770-783 NN denotes visualization
T12360 784-787 VBD denotes was
T12361 788-792 IN denotes with
T12362 793-795 DT denotes an
T12364 796-801 NNP denotes Alexa
T12365 802-807 NNP denotes Fluor
T12367 808-811 CD denotes 488
T12368 811-812 HYPH denotes -
T12366 812-822 VBN denotes conjugated
T12369 823-833 JJ denotes anti-mouse
T12363 834-837 NN denotes IgG
T12370 837-838 . denotes .
T12371 838-962 sentence denotes (D) An intense Annexin A7 immunostaining is detectable in the neuroepithelium of the neural tube (a, lumen of neural tube).
T12372 839-840 -LRB- denotes (
T12373 840-841 LS denotes D
T12375 841-842 -RRB- denotes )
T12376 843-845 DT denotes An
T12378 846-853 JJ denotes intense
T12379 854-861 NN denotes Annexin
T12380 862-864 NN denotes A7
T12377 865-879 NN denotes immunostaining
T12374 880-882 VBZ denotes is
T12381 883-893 JJ denotes detectable
T12382 894-896 IN denotes in
T12383 897-900 DT denotes the
T12384 901-916 NN denotes neuroepithelium
T12385 917-919 IN denotes of
T12386 920-923 DT denotes the
T12388 924-930 JJ denotes neural
T12387 931-935 NN denotes tube
T12389 936-937 -LRB- denotes (
T12391 937-938 NN denotes a
T12392 938-940 , denotes ,
T12390 940-945 NN denotes lumen
T12393 946-948 IN denotes of
T12394 949-955 JJ denotes neural
T12395 956-960 NN denotes tube
T12396 960-961 -RRB- denotes )
T12397 961-962 . denotes .
T12398 962-1063 sentence denotes (E,F) Higher magnifications of the neuroepithelium show that Annexin A7 is expressed in the cytosol.
T12399 963-964 -LRB- denotes (
T12400 964-965 LS denotes E
T12402 965-966 , denotes ,
T12403 966-967 LS denotes F
T12404 967-968 -RRB- denotes )
T12405 969-975 JJR denotes Higher
T12406 976-990 NNS denotes magnifications
T12407 991-993 IN denotes of
T12408 994-997 DT denotes the
T12409 998-1013 NN denotes neuroepithelium
T12401 1014-1018 VBP denotes show
T12410 1019-1023 IN denotes that
T12412 1024-1031 NN denotes Annexin
T12413 1032-1034 NN denotes A7
T12414 1035-1037 VBZ denotes is
T12411 1038-1047 VBN denotes expressed
T12415 1048-1050 IN denotes in
T12416 1051-1054 DT denotes the
T12417 1055-1062 NN denotes cytosol
T12418 1062-1063 . denotes .
T12419 1063-1127 sentence denotes Arrowheads point to Annexin A7 immunoreactivity in the cytosol.
T12420 1064-1074 NNS denotes Arrowheads
T12421 1075-1080 VBP denotes point
T12422 1081-1083 IN denotes to
T12423 1084-1091 NN denotes Annexin
T12424 1092-1094 NN denotes A7
T12425 1095-1111 NN denotes immunoreactivity
T12426 1112-1114 IN denotes in
T12427 1115-1118 DT denotes the
T12428 1119-1126 NN denotes cytosol
T12429 1126-1127 . denotes .
T12430 1127-1179 sentence denotes (G) Phase contrast, embryo E13, caudal neural tube.
T12431 1128-1129 -LRB- denotes (
T12433 1129-1130 LS denotes G
T12434 1130-1131 -RRB- denotes )
T12435 1132-1137 NN denotes Phase
T12436 1138-1146 NN denotes contrast
T12437 1146-1148 , denotes ,
T12438 1148-1154 NN denotes embryo
T12439 1155-1158 NN denotes E13
T12440 1158-1160 , denotes ,
T12441 1160-1166 JJ denotes caudal
T12442 1167-1173 JJ denotes neural
T12432 1174-1178 NN denotes tube
T12443 1178-1179 . denotes .
T12444 1179-1310 sentence denotes (H) Immunostaining of the paraffin section was performed using purified mAb 203–217 and Alexa Fluor 488-conjugated anti-mouse IgG.
T12445 1180-1181 -LRB- denotes (
T12446 1181-1182 LS denotes H
T12448 1182-1183 -RRB- denotes )
T12449 1184-1198 NN denotes Immunostaining
T12450 1199-1201 IN denotes of
T12451 1202-1205 DT denotes the
T12453 1206-1214 NN denotes paraffin
T12452 1215-1222 NN denotes section
T12454 1223-1226 VBD denotes was
T12447 1227-1236 VBN denotes performed
T12455 1237-1242 VBG denotes using
T12456 1243-1251 VBN denotes purified
T12458 1252-1255 NN denotes mAb
T12457 1256-1259 CD denotes 203
T12459 1259-1260 SYM denotes –
T12460 1260-1263 CD denotes 217
T12461 1264-1267 CC denotes and
T12462 1268-1273 NNP denotes Alexa
T12463 1274-1279 NNP denotes Fluor
T12465 1280-1283 CD denotes 488
T12466 1283-1284 HYPH denotes -
T12464 1284-1294 VBN denotes conjugated
T12468 1295-1305 JJ denotes anti-mouse
T12467 1306-1309 NN denotes IgG
T12469 1309-1310 . denotes .
T12470 1310-1382 sentence denotes (I) Negative control using the secondary Alexa Fluor 488-antibody only.
T12471 1311-1312 -LRB- denotes (
T12472 1312-1313 LS denotes I
T12474 1313-1314 -RRB- denotes )
T12475 1315-1323 JJ denotes Negative
T12473 1324-1331 NN denotes control
T12476 1332-1337 VBG denotes using
T12477 1338-1341 DT denotes the
T12479 1342-1351 JJ denotes secondary
T12480 1352-1357 NNP denotes Alexa
T12481 1358-1363 NNP denotes Fluor
T12482 1364-1367 CD denotes 488
T12483 1367-1368 HYPH denotes -
T12478 1368-1376 NN denotes antibody
T12484 1377-1381 RB denotes only
T12485 1381-1382 . denotes .
T12486 1382-1394 sentence denotes Bar, 20 μm.
T12487 1383-1386 NN denotes Bar
T12488 1386-1388 , denotes ,
T12489 1388-1390 CD denotes 20
T12490 1391-1393 NN denotes μm
T12491 1393-1394 . denotes .
R5724 T12193 T12194 compound Annexin,A7
R5725 T12194 T12195 compound A7,immunoreactivity
R5726 T12196 T12195 prep in,immunoreactivity
R5727 T12197 T12198 amod early,embryos
R5728 T12198 T12196 pobj embryos,in
R5729 T12199 T12198 compound mouse,embryos
R5730 T12200 T12195 punct .,immunoreactivity
R5731 T12202 T12203 punct (,A
R5732 T12203 T12204 meta A,consists
R5733 T12205 T12203 punct ),A
R5734 T12206 T12207 compound Phase,contrast
R5735 T12207 T12204 dep contrast,consists
R5736 T12208 T12204 punct ", ",consists
R5737 T12209 T12210 compound embryo,E5
R5738 T12210 T12204 dep E5,consists
R5739 T12211 T12204 punct : ,consists
R5740 T12212 T12213 det The,cylinder
R5741 T12213 T12204 nsubj cylinder,consists
R5742 T12214 T12213 compound egg,cylinder
R5743 T12215 T12204 prep of,consists
R5744 T12216 T12217 det an,mass
R5745 T12217 T12215 pobj mass,of
R5746 T12218 T12217 amod inner,mass
R5747 T12219 T12217 compound cell,mass
R5748 T12220 T12221 punct (,a
R5749 T12221 T12217 parataxis a,mass
R5750 T12222 T12221 punct ),a
R5751 T12223 T12217 acl representing,mass
R5752 T12224 T12225 det the,ectoderm
R5753 T12225 T12223 dobj ectoderm,representing
R5754 T12226 T12217 cc and,mass
R5755 T12227 T12228 det an,layer
R5756 T12228 T12217 conj layer,mass
R5757 T12229 T12228 amod outer,layer
R5758 T12230 T12228 prep of,layer
R5759 T12231 T12232 compound endoderm,cells
R5760 T12232 T12230 pobj cells,of
R5761 T12233 T12234 punct (,b
R5762 T12234 T12228 parataxis b,layer
R5763 T12235 T12234 punct ),b
R5764 T12236 T12204 punct .,consists
R5765 T12238 T12239 punct (,B
R5766 T12239 T12240 meta B,performed
R5767 T12241 T12239 punct ),B
R5768 T12242 T12240 nsubjpass Immunostaining,performed
R5769 T12243 T12242 prep of,Immunostaining
R5770 T12244 T12245 det the,section
R5771 T12245 T12243 pobj section,of
R5772 T12246 T12245 compound paraffin,section
R5773 T12247 T12240 auxpass was,performed
R5774 T12248 T12249 aux using,purified
R5775 T12249 T12240 advcl purified,performed
R5776 T12250 T12251 nmod mAb,203
R5777 T12251 T12249 dobj 203,purified
R5778 T12252 T12253 punct –,217
R5779 T12253 T12251 prep 217,203
R5780 T12254 T12251 cc and,203
R5781 T12255 T12256 npadvmod Cy3,conjugated
R5782 T12256 T12258 amod conjugated,IgG
R5783 T12257 T12256 punct -,conjugated
R5784 T12258 T12251 conj IgG,203
R5785 T12259 T12258 amod anti-mouse,IgG
R5786 T12260 T12240 punct .,performed
R5787 T12262 T12263 compound Annexin,A7
R5788 T12263 T12264 nsubjpass A7,expressed
R5789 T12265 T12264 auxpass is,expressed
R5790 T12266 T12264 prep in,expressed
R5791 T12267 T12268 det both,types
R5792 T12268 T12266 pobj types,in
R5793 T12269 T12268 compound cell,types
R5794 T12270 T12268 prep of,types
R5795 T12271 T12272 det the,cylinder
R5796 T12272 T12270 pobj cylinder,of
R5797 T12273 T12272 compound egg,cylinder
R5798 T12274 T12264 prep with,expressed
R5799 T12275 T12276 det a,staining
R5800 T12276 T12274 pobj staining,with
R5801 T12277 T12276 amod strong,staining
R5802 T12278 T12276 prep of,staining
R5803 T12279 T12280 det the,endoderm
R5804 T12280 T12278 pobj endoderm,of
R5805 T12281 T12276 cc and,staining
R5806 T12282 T12283 det a,staining
R5807 T12283 T12276 conj staining,staining
R5808 T12284 T12283 amod weaker,staining
R5809 T12285 T12283 prep of,staining
R5810 T12286 T12287 det the,ectoderm
R5811 T12287 T12285 pobj ectoderm,of
R5812 T12288 T12264 punct .,expressed
R5813 T12290 T12291 det The,nuclei
R5814 T12291 T12292 nsubj nuclei,are
R5815 T12293 T12292 acomp devoid,are
R5816 T12294 T12293 prep of,devoid
R5817 T12295 T12296 amod immune,reactions
R5818 T12296 T12294 pobj reactions,of
R5819 T12297 T12292 punct .,are
R5820 T12299 T12300 punct (,control
R5821 T12301 T12300 meta C,control
R5822 T12302 T12300 punct ),control
R5823 T12303 T12300 amod Negative,control
R5824 T12304 T12300 acl using,control
R5825 T12305 T12306 det the,antibody
R5826 T12306 T12304 dobj antibody,using
R5827 T12307 T12306 amod secondary,antibody
R5828 T12308 T12306 compound Cy3,antibody
R5829 T12309 T12306 punct -,antibody
R5830 T12310 T12306 advmod only,antibody
R5831 T12311 T12300 punct .,control
R5832 T12313 T12314 punct (,D
R5833 T12314 T12315 meta D,section
R5834 T12316 T12314 punct -,D
R5835 T12317 T12314 dep F,D
R5836 T12318 T12314 punct ),D
R5837 T12319 T12320 compound Annexin,A7
R5838 T12320 T12321 compound A7,expression
R5839 T12321 T12315 dep expression,section
R5840 T12322 T12321 prep in,expression
R5841 T12323 T12324 det the,tube
R5842 T12324 T12322 pobj tube,in
R5843 T12325 T12324 amod proximal,tube
R5844 T12326 T12324 amod neural,tube
R5845 T12327 T12328 punct (,D
R5846 T12328 T12324 parataxis D,tube
R5847 T12329 T12328 punct ),D
R5848 T12330 T12324 cc and,tube
R5849 T12331 T12332 amod nearby,fold
R5850 T12332 T12324 conj fold,tube
R5851 T12333 T12332 amod neural,fold
R5852 T12334 T12335 punct (,F
R5853 T12335 T12332 parataxis F,fold
R5854 T12336 T12335 nmod E,F
R5855 T12337 T12335 punct ",",F
R5856 T12338 T12335 punct ),F
R5857 T12339 T12315 punct ", ",section
R5858 T12340 T12341 compound embryo,E8
R5859 T12341 T12315 dep E8,section
R5860 T12342 T12315 punct ", ",section
R5861 T12343 T12315 amod transverse,section
R5862 T12344 T12315 punct .,section
R5863 T12346 T12347 nsubjpass Immunolabeling,performed
R5864 T12348 T12346 prep of,Immunolabeling
R5865 T12349 T12350 compound Annexin,A7
R5866 T12350 T12348 pobj A7,of
R5867 T12351 T12347 auxpass was,performed
R5868 T12352 T12347 prep with,performed
R5869 T12353 T12354 amod purified,203
R5870 T12354 T12352 pobj 203,with
R5871 T12355 T12354 nmod mAb,203
R5872 T12356 T12357 punct –,217
R5873 T12357 T12354 prep 217,203
R5874 T12358 T12347 cc and,performed
R5875 T12359 T12360 nsubj visualization,was
R5876 T12360 T12347 conj was,performed
R5877 T12361 T12360 prep with,was
R5878 T12362 T12363 det an,IgG
R5879 T12363 T12361 pobj IgG,with
R5880 T12364 T12365 compound Alexa,Fluor
R5881 T12365 T12366 npadvmod Fluor,conjugated
R5882 T12366 T12363 amod conjugated,IgG
R5883 T12367 T12365 nummod 488,Fluor
R5884 T12368 T12366 punct -,conjugated
R5885 T12369 T12363 amod anti-mouse,IgG
R5886 T12370 T12360 punct .,was
R5887 T12372 T12373 punct (,D
R5888 T12373 T12374 meta D,is
R5889 T12375 T12373 punct ),D
R5890 T12376 T12377 det An,immunostaining
R5891 T12377 T12374 nsubj immunostaining,is
R5892 T12378 T12377 amod intense,immunostaining
R5893 T12379 T12380 compound Annexin,A7
R5894 T12380 T12377 compound A7,immunostaining
R5895 T12381 T12374 acomp detectable,is
R5896 T12382 T12374 prep in,is
R5897 T12383 T12384 det the,neuroepithelium
R5898 T12384 T12382 pobj neuroepithelium,in
R5899 T12385 T12384 prep of,neuroepithelium
R5900 T12386 T12387 det the,tube
R5901 T12387 T12385 pobj tube,of
R5902 T12388 T12387 amod neural,tube
R5903 T12389 T12390 punct (,lumen
R5904 T12390 T12374 parataxis lumen,is
R5905 T12391 T12390 dep a,lumen
R5906 T12392 T12390 punct ", ",lumen
R5907 T12393 T12390 prep of,lumen
R5908 T12394 T12395 amod neural,tube
R5909 T12395 T12393 pobj tube,of
R5910 T12396 T12390 punct ),lumen
R5911 T12397 T12374 punct .,is
R5912 T12399 T12400 punct (,E
R5913 T12400 T12401 meta E,show
R5914 T12402 T12400 punct ",",E
R5915 T12403 T12400 dep F,E
R5916 T12404 T12400 punct ),E
R5917 T12405 T12406 amod Higher,magnifications
R5918 T12406 T12401 nsubj magnifications,show
R5919 T12407 T12406 prep of,magnifications
R5920 T12408 T12409 det the,neuroepithelium
R5921 T12409 T12407 pobj neuroepithelium,of
R5922 T12410 T12411 mark that,expressed
R5923 T12411 T12401 ccomp expressed,show
R5924 T12412 T12413 compound Annexin,A7
R5925 T12413 T12411 nsubjpass A7,expressed
R5926 T12414 T12411 auxpass is,expressed
R5927 T12415 T12411 prep in,expressed
R5928 T12416 T12417 det the,cytosol
R5929 T12417 T12415 pobj cytosol,in
R5930 T12418 T12401 punct .,show
R5931 T12420 T12421 nsubj Arrowheads,point
R5932 T12422 T12421 prep to,point
R5933 T12423 T12424 compound Annexin,A7
R5934 T12424 T12425 compound A7,immunoreactivity
R5935 T12425 T12422 pobj immunoreactivity,to
R5936 T12426 T12421 prep in,point
R5937 T12427 T12428 det the,cytosol
R5938 T12428 T12426 pobj cytosol,in
R5939 T12429 T12421 punct .,point
R5940 T12431 T12432 punct (,tube
R5941 T12433 T12432 meta G,tube
R5942 T12434 T12432 punct ),tube
R5943 T12435 T12436 compound Phase,contrast
R5944 T12436 T12432 dep contrast,tube
R5945 T12437 T12432 punct ", ",tube
R5946 T12438 T12439 compound embryo,E13
R5947 T12439 T12432 dep E13,tube
R5948 T12440 T12432 punct ", ",tube
R5949 T12441 T12432 amod caudal,tube
R5950 T12442 T12432 amod neural,tube
R5951 T12443 T12432 punct .,tube
R5952 T12445 T12446 punct (,H
R5953 T12446 T12447 meta H,performed
R5954 T12448 T12446 punct ),H
R5955 T12449 T12447 nsubjpass Immunostaining,performed
R5956 T12450 T12449 prep of,Immunostaining
R5957 T12451 T12452 det the,section
R5958 T12452 T12450 pobj section,of
R5959 T12453 T12452 compound paraffin,section
R5960 T12454 T12447 auxpass was,performed
R5961 T12455 T12447 advcl using,performed
R5962 T12456 T12457 amod purified,203
R5963 T12457 T12455 dobj 203,using
R5964 T12458 T12457 nmod mAb,203
R5965 T12459 T12460 punct –,217
R5966 T12460 T12457 prep 217,203
R5967 T12461 T12457 cc and,203
R5968 T12462 T12463 compound Alexa,Fluor
R5969 T12463 T12464 npadvmod Fluor,conjugated
R5970 T12464 T12467 amod conjugated,IgG
R5971 T12465 T12463 nummod 488,Fluor
R5972 T12466 T12464 punct -,conjugated
R5973 T12467 T12457 conj IgG,203
R5974 T12468 T12467 amod anti-mouse,IgG
R5975 T12469 T12447 punct .,performed
R5976 T12471 T12472 punct (,I
R5977 T12472 T12473 meta I,control
R5978 T12474 T12472 punct ),I
R5979 T12475 T12473 amod Negative,control
R5980 T12476 T12473 acl using,control
R5981 T12477 T12478 det the,antibody
R5982 T12478 T12476 dobj antibody,using
R5983 T12479 T12478 amod secondary,antibody
R5984 T12480 T12481 nmod Alexa,Fluor
R5985 T12481 T12478 nmod Fluor,antibody
R5986 T12482 T12481 nummod 488,Fluor
R5987 T12483 T12478 punct -,antibody
R5988 T12484 T12478 advmod only,antibody
R5989 T12485 T12473 punct .,control
R5990 T12488 T12487 punct ", ",Bar
R5991 T12489 T12490 nummod 20,μm
R5992 T12490 T12487 appos μm,Bar
R5993 T12491 T12487 punct .,Bar