| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T11654 |
10-20 |
NN |
denotes |
Expression |
| T11655 |
21-23 |
IN |
denotes |
of |
| T11656 |
24-31 |
NN |
denotes |
Annexin |
| T11657 |
32-34 |
NN |
denotes |
A7 |
| T11658 |
34-35 |
. |
denotes |
. |
| T11659 |
35-156 |
sentence |
denotes |
(A) Northern blot analysis of RNA from ES-cells and embryos E7, E11, E15, E17 probed with a full length annexin A7 cDNA. |
| T11660 |
36-37 |
-LRB- |
denotes |
( |
| T11661 |
37-38 |
LS |
denotes |
A |
| T11663 |
38-39 |
-RRB- |
denotes |
) |
| T11664 |
40-48 |
NNP |
denotes |
Northern |
| T11665 |
49-53 |
NN |
denotes |
blot |
| T11662 |
54-62 |
NN |
denotes |
analysis |
| T11666 |
63-65 |
IN |
denotes |
of |
| T11667 |
66-69 |
NN |
denotes |
RNA |
| T11668 |
70-74 |
IN |
denotes |
from |
| T11669 |
75-77 |
NN |
denotes |
ES |
| T11671 |
77-78 |
HYPH |
denotes |
- |
| T11670 |
78-83 |
NNS |
denotes |
cells |
| T11672 |
84-87 |
CC |
denotes |
and |
| T11673 |
88-95 |
NNS |
denotes |
embryos |
| T11675 |
96-98 |
NN |
denotes |
E7 |
| T11676 |
98-100 |
, |
denotes |
, |
| T11677 |
100-103 |
NN |
denotes |
E11 |
| T11678 |
103-105 |
, |
denotes |
, |
| T11679 |
105-108 |
NN |
denotes |
E15 |
| T11680 |
108-110 |
, |
denotes |
, |
| T11674 |
110-113 |
NN |
denotes |
E17 |
| T11681 |
114-120 |
VBD |
denotes |
probed |
| T11682 |
121-125 |
IN |
denotes |
with |
| T11683 |
126-127 |
DT |
denotes |
a |
| T11685 |
128-132 |
JJ |
denotes |
full |
| T11686 |
133-139 |
NN |
denotes |
length |
| T11687 |
140-147 |
NN |
denotes |
annexin |
| T11688 |
148-150 |
NN |
denotes |
A7 |
| T11684 |
151-155 |
NN |
denotes |
cDNA |
| T11689 |
155-156 |
. |
denotes |
. |
| T11690 |
156-237 |
sentence |
denotes |
Transcripts of 1.8 and 2.4 kb are detected in all stages examined (upper panel). |
| T11691 |
157-168 |
NNS |
denotes |
Transcripts |
| T11693 |
169-171 |
IN |
denotes |
of |
| T11694 |
172-175 |
CD |
denotes |
1.8 |
| T11696 |
176-179 |
CC |
denotes |
and |
| T11697 |
180-183 |
CD |
denotes |
2.4 |
| T11695 |
184-186 |
NN |
denotes |
kb |
| T11698 |
187-190 |
VBP |
denotes |
are |
| T11692 |
191-199 |
VBN |
denotes |
detected |
| T11699 |
200-202 |
IN |
denotes |
in |
| T11700 |
203-206 |
DT |
denotes |
all |
| T11701 |
207-213 |
NNS |
denotes |
stages |
| T11702 |
214-222 |
VBN |
denotes |
examined |
| T11703 |
223-224 |
-LRB- |
denotes |
( |
| T11705 |
224-229 |
JJ |
denotes |
upper |
| T11704 |
230-235 |
NN |
denotes |
panel |
| T11706 |
235-236 |
-RRB- |
denotes |
) |
| T11707 |
236-237 |
. |
denotes |
. |
| T11708 |
237-319 |
sentence |
denotes |
Reprobing with a β-actin probe verified equal loading (lower panel, 2.0 kb band). |
| T11709 |
238-247 |
VBG |
denotes |
Reprobing |
| T11711 |
248-252 |
IN |
denotes |
with |
| T11712 |
253-254 |
DT |
denotes |
a |
| T11714 |
255-256 |
NN |
denotes |
β |
| T11716 |
256-257 |
HYPH |
denotes |
- |
| T11715 |
257-262 |
NN |
denotes |
actin |
| T11713 |
263-268 |
NN |
denotes |
probe |
| T11710 |
269-277 |
VBD |
denotes |
verified |
| T11717 |
278-283 |
JJ |
denotes |
equal |
| T11718 |
284-291 |
NN |
denotes |
loading |
| T11719 |
292-293 |
-LRB- |
denotes |
( |
| T11721 |
293-298 |
JJ |
denotes |
lower |
| T11722 |
299-304 |
NN |
denotes |
panel |
| T11723 |
304-306 |
, |
denotes |
, |
| T11724 |
306-309 |
CD |
denotes |
2.0 |
| T11725 |
310-312 |
NN |
denotes |
kb |
| T11720 |
313-317 |
NN |
denotes |
band |
| T11726 |
317-318 |
-RRB- |
denotes |
) |
| T11727 |
318-319 |
. |
denotes |
. |
| T11728 |
319-387 |
sentence |
denotes |
(B) Murine ES cells (ES) express only the small Annexin A7 isoform. |
| T11729 |
320-321 |
-LRB- |
denotes |
( |
| T11730 |
321-322 |
LS |
denotes |
B |
| T11732 |
322-323 |
-RRB- |
denotes |
) |
| T11733 |
324-330 |
JJ |
denotes |
Murine |
| T11735 |
331-333 |
NN |
denotes |
ES |
| T11734 |
334-339 |
NNS |
denotes |
cells |
| T11736 |
340-341 |
-LRB- |
denotes |
( |
| T11737 |
341-343 |
NN |
denotes |
ES |
| T11738 |
343-344 |
-RRB- |
denotes |
) |
| T11731 |
345-352 |
VBP |
denotes |
express |
| T11739 |
353-357 |
RB |
denotes |
only |
| T11741 |
358-361 |
DT |
denotes |
the |
| T11742 |
362-367 |
JJ |
denotes |
small |
| T11743 |
368-375 |
NN |
denotes |
Annexin |
| T11744 |
376-378 |
NN |
denotes |
A7 |
| T11740 |
379-386 |
NN |
denotes |
isoform |
| T11745 |
386-387 |
. |
denotes |
. |
| T11746 |
387-506 |
sentence |
denotes |
Both isoforms are expressed in neuroblastoma neuro-2a cells (N2A), heart (HRT) and brain tissue (CNS) from adult mice. |
| T11747 |
388-392 |
DT |
denotes |
Both |
| T11748 |
393-401 |
NNS |
denotes |
isoforms |
| T11750 |
402-405 |
VBP |
denotes |
are |
| T11749 |
406-415 |
VBN |
denotes |
expressed |
| T11751 |
416-418 |
IN |
denotes |
in |
| T11752 |
419-432 |
NN |
denotes |
neuroblastoma |
| T11754 |
433-438 |
NN |
denotes |
neuro |
| T11756 |
438-439 |
HYPH |
denotes |
- |
| T11755 |
439-441 |
NN |
denotes |
2a |
| T11753 |
442-447 |
NNS |
denotes |
cells |
| T11757 |
448-449 |
-LRB- |
denotes |
( |
| T11758 |
449-452 |
NN |
denotes |
N2A |
| T11759 |
452-453 |
-RRB- |
denotes |
) |
| T11760 |
453-455 |
, |
denotes |
, |
| T11761 |
455-460 |
NN |
denotes |
heart |
| T11762 |
461-462 |
-LRB- |
denotes |
( |
| T11763 |
462-465 |
NN |
denotes |
HRT |
| T11764 |
465-466 |
-RRB- |
denotes |
) |
| T11765 |
467-470 |
CC |
denotes |
and |
| T11766 |
471-476 |
NN |
denotes |
brain |
| T11767 |
477-483 |
NN |
denotes |
tissue |
| T11768 |
484-485 |
-LRB- |
denotes |
( |
| T11769 |
485-488 |
NN |
denotes |
CNS |
| T11770 |
488-489 |
-RRB- |
denotes |
) |
| T11771 |
490-494 |
IN |
denotes |
from |
| T11772 |
495-500 |
JJ |
denotes |
adult |
| T11773 |
501-505 |
NNS |
denotes |
mice |
| T11774 |
505-506 |
. |
denotes |
. |
| T11775 |
506-677 |
sentence |
denotes |
Proteins from neuro-2a and ES cells, heart and brain were resolved by SDS polyacrylamide gel electrophoresis (12% acrylamide) and transferred to nitrocellulose membranes. |
| T11776 |
507-515 |
NNS |
denotes |
Proteins |
| T11778 |
516-520 |
IN |
denotes |
from |
| T11779 |
521-526 |
NN |
denotes |
neuro |
| T11781 |
526-527 |
HYPH |
denotes |
- |
| T11780 |
527-529 |
NN |
denotes |
2a |
| T11782 |
530-533 |
CC |
denotes |
and |
| T11783 |
534-536 |
NN |
denotes |
ES |
| T11784 |
537-542 |
NNS |
denotes |
cells |
| T11785 |
542-544 |
, |
denotes |
, |
| T11786 |
544-549 |
NN |
denotes |
heart |
| T11787 |
550-553 |
CC |
denotes |
and |
| T11788 |
554-559 |
NN |
denotes |
brain |
| T11789 |
560-564 |
VBD |
denotes |
were |
| T11777 |
565-573 |
VBN |
denotes |
resolved |
| T11790 |
574-576 |
IN |
denotes |
by |
| T11791 |
577-580 |
NN |
denotes |
SDS |
| T11793 |
581-595 |
NN |
denotes |
polyacrylamide |
| T11794 |
596-599 |
NN |
denotes |
gel |
| T11792 |
600-615 |
NN |
denotes |
electrophoresis |
| T11795 |
616-617 |
-LRB- |
denotes |
( |
| T11797 |
617-619 |
CD |
denotes |
12 |
| T11798 |
619-620 |
NN |
denotes |
% |
| T11796 |
621-631 |
NN |
denotes |
acrylamide |
| T11799 |
631-632 |
-RRB- |
denotes |
) |
| T11800 |
633-636 |
CC |
denotes |
and |
| T11801 |
637-648 |
VBN |
denotes |
transferred |
| T11802 |
649-651 |
IN |
denotes |
to |
| T11803 |
652-666 |
NN |
denotes |
nitrocellulose |
| T11804 |
667-676 |
NNS |
denotes |
membranes |
| T11805 |
676-677 |
. |
denotes |
. |
| T11806 |
677-791 |
sentence |
denotes |
The Western blot was probed with mAb 203–217 followed by incubation with a peroxidase coupled secondary antibody. |
| T11807 |
678-681 |
DT |
denotes |
The |
| T11809 |
682-689 |
NNP |
denotes |
Western |
| T11808 |
690-694 |
NN |
denotes |
blot |
| T11811 |
695-698 |
VBD |
denotes |
was |
| T11810 |
699-705 |
VBN |
denotes |
probed |
| T11812 |
706-710 |
IN |
denotes |
with |
| T11813 |
711-714 |
NN |
denotes |
mAb |
| T11814 |
715-718 |
CD |
denotes |
203 |
| T11815 |
718-719 |
SYM |
denotes |
– |
| T11816 |
719-722 |
CD |
denotes |
217 |
| T11817 |
723-731 |
VBN |
denotes |
followed |
| T11818 |
732-734 |
IN |
denotes |
by |
| T11819 |
735-745 |
NN |
denotes |
incubation |
| T11820 |
746-750 |
IN |
denotes |
with |
| T11821 |
751-752 |
DT |
denotes |
a |
| T11823 |
753-763 |
NN |
denotes |
peroxidase |
| T11824 |
764-771 |
VBN |
denotes |
coupled |
| T11825 |
772-781 |
JJ |
denotes |
secondary |
| T11822 |
782-790 |
NN |
denotes |
antibody |
| T11826 |
790-791 |
. |
denotes |
. |
| T11827 |
791-838 |
sentence |
denotes |
Detection was with enhanced chemiluminescence. |
| T11828 |
792-801 |
NN |
denotes |
Detection |
| T11829 |
802-805 |
VBD |
denotes |
was |
| T11830 |
806-810 |
IN |
denotes |
with |
| T11831 |
811-819 |
VBN |
denotes |
enhanced |
| T11832 |
820-837 |
NN |
denotes |
chemiluminescence |
| T11833 |
837-838 |
. |
denotes |
. |
| R5559 |
T11655 |
T11654 |
prep |
of,Expression |
| R5560 |
T11656 |
T11657 |
compound |
Annexin,A7 |
| R5561 |
T11657 |
T11655 |
pobj |
A7,of |
| R5562 |
T11658 |
T11654 |
punct |
.,Expression |
| R5563 |
T11660 |
T11661 |
punct |
(,A |
| R5564 |
T11661 |
T11662 |
meta |
A,analysis |
| R5565 |
T11663 |
T11661 |
punct |
),A |
| R5566 |
T11664 |
T11665 |
compound |
Northern,blot |
| R5567 |
T11665 |
T11662 |
compound |
blot,analysis |
| R5568 |
T11666 |
T11662 |
prep |
of,analysis |
| R5569 |
T11667 |
T11666 |
pobj |
RNA,of |
| R5570 |
T11668 |
T11667 |
prep |
from,RNA |
| R5571 |
T11669 |
T11670 |
compound |
ES,cells |
| R5572 |
T11670 |
T11668 |
pobj |
cells,from |
| R5573 |
T11671 |
T11670 |
punct |
-,cells |
| R5574 |
T11672 |
T11670 |
cc |
and,cells |
| R5575 |
T11673 |
T11674 |
nmod |
embryos,E17 |
| R5576 |
T11674 |
T11670 |
conj |
E17,cells |
| R5577 |
T11675 |
T11674 |
nmod |
E7,E17 |
| R5578 |
T11676 |
T11674 |
punct |
", ",E17 |
| R5579 |
T11677 |
T11674 |
nmod |
E11,E17 |
| R5580 |
T11678 |
T11674 |
punct |
", ",E17 |
| R5581 |
T11679 |
T11674 |
nmod |
E15,E17 |
| R5582 |
T11680 |
T11674 |
punct |
", ",E17 |
| R5583 |
T11681 |
T11667 |
acl |
probed,RNA |
| R5584 |
T11682 |
T11681 |
prep |
with,probed |
| R5585 |
T11683 |
T11684 |
det |
a,cDNA |
| R5586 |
T11684 |
T11682 |
pobj |
cDNA,with |
| R5587 |
T11685 |
T11686 |
amod |
full,length |
| R5588 |
T11686 |
T11684 |
compound |
length,cDNA |
| R5589 |
T11687 |
T11688 |
compound |
annexin,A7 |
| R5590 |
T11688 |
T11684 |
compound |
A7,cDNA |
| R5591 |
T11689 |
T11662 |
punct |
.,analysis |
| R5592 |
T11691 |
T11692 |
nsubjpass |
Transcripts,detected |
| R5593 |
T11693 |
T11691 |
prep |
of,Transcripts |
| R5594 |
T11694 |
T11695 |
nummod |
1.8,kb |
| R5595 |
T11695 |
T11693 |
pobj |
kb,of |
| R5596 |
T11696 |
T11694 |
cc |
and,1.8 |
| R5597 |
T11697 |
T11694 |
conj |
2.4,1.8 |
| R5598 |
T11698 |
T11692 |
auxpass |
are,detected |
| R5599 |
T11699 |
T11692 |
prep |
in,detected |
| R5600 |
T11700 |
T11701 |
det |
all,stages |
| R5601 |
T11701 |
T11699 |
pobj |
stages,in |
| R5602 |
T11702 |
T11701 |
acl |
examined,stages |
| R5603 |
T11703 |
T11704 |
punct |
(,panel |
| R5604 |
T11704 |
T11692 |
parataxis |
panel,detected |
| R5605 |
T11705 |
T11704 |
amod |
upper,panel |
| R5606 |
T11706 |
T11704 |
punct |
),panel |
| R5607 |
T11707 |
T11692 |
punct |
.,detected |
| R5608 |
T11709 |
T11710 |
nsubj |
Reprobing,verified |
| R5609 |
T11711 |
T11709 |
prep |
with,Reprobing |
| R5610 |
T11712 |
T11713 |
det |
a,probe |
| R5611 |
T11713 |
T11711 |
pobj |
probe,with |
| R5612 |
T11714 |
T11715 |
compound |
β,actin |
| R5613 |
T11715 |
T11713 |
compound |
actin,probe |
| R5614 |
T11716 |
T11715 |
punct |
-,actin |
| R5615 |
T11717 |
T11718 |
amod |
equal,loading |
| R5616 |
T11718 |
T11710 |
dobj |
loading,verified |
| R5617 |
T11719 |
T11720 |
punct |
(,band |
| R5618 |
T11720 |
T11710 |
parataxis |
band,verified |
| R5619 |
T11721 |
T11722 |
amod |
lower,panel |
| R5620 |
T11722 |
T11720 |
dep |
panel,band |
| R5621 |
T11723 |
T11720 |
punct |
", ",band |
| R5622 |
T11724 |
T11725 |
nummod |
2.0,kb |
| R5623 |
T11725 |
T11720 |
compound |
kb,band |
| R5624 |
T11726 |
T11720 |
punct |
),band |
| R5625 |
T11727 |
T11710 |
punct |
.,verified |
| R5626 |
T11729 |
T11730 |
punct |
(,B |
| R5627 |
T11730 |
T11731 |
meta |
B,express |
| R5628 |
T11732 |
T11730 |
punct |
),B |
| R5629 |
T11733 |
T11734 |
amod |
Murine,cells |
| R5630 |
T11734 |
T11731 |
nsubj |
cells,express |
| R5631 |
T11735 |
T11734 |
compound |
ES,cells |
| R5632 |
T11736 |
T11734 |
punct |
(,cells |
| R5633 |
T11737 |
T11734 |
appos |
ES,cells |
| R5634 |
T11738 |
T11731 |
punct |
),express |
| R5635 |
T11739 |
T11740 |
advmod |
only,isoform |
| R5636 |
T11740 |
T11731 |
dobj |
isoform,express |
| R5637 |
T11741 |
T11740 |
det |
the,isoform |
| R5638 |
T11742 |
T11740 |
amod |
small,isoform |
| R5639 |
T11743 |
T11744 |
compound |
Annexin,A7 |
| R5640 |
T11744 |
T11740 |
compound |
A7,isoform |
| R5641 |
T11745 |
T11731 |
punct |
.,express |
| R5642 |
T11747 |
T11748 |
det |
Both,isoforms |
| R5643 |
T11748 |
T11749 |
nsubjpass |
isoforms,expressed |
| R5644 |
T11750 |
T11749 |
auxpass |
are,expressed |
| R5645 |
T11751 |
T11749 |
prep |
in,expressed |
| R5646 |
T11752 |
T11753 |
compound |
neuroblastoma,cells |
| R5647 |
T11753 |
T11751 |
pobj |
cells,in |
| R5648 |
T11754 |
T11755 |
compound |
neuro,2a |
| R5649 |
T11755 |
T11753 |
compound |
2a,cells |
| R5650 |
T11756 |
T11755 |
punct |
-,2a |
| R5651 |
T11757 |
T11753 |
punct |
(,cells |
| R5652 |
T11758 |
T11753 |
appos |
N2A,cells |
| R5653 |
T11759 |
T11753 |
punct |
),cells |
| R5654 |
T11760 |
T11753 |
punct |
", ",cells |
| R5655 |
T11761 |
T11753 |
conj |
heart,cells |
| R5656 |
T11762 |
T11761 |
punct |
(,heart |
| R5657 |
T11763 |
T11761 |
appos |
HRT,heart |
| R5658 |
T11764 |
T11761 |
punct |
),heart |
| R5659 |
T11765 |
T11761 |
cc |
and,heart |
| R5660 |
T11766 |
T11767 |
compound |
brain,tissue |
| R5661 |
T11767 |
T11761 |
conj |
tissue,heart |
| R5662 |
T11768 |
T11767 |
punct |
(,tissue |
| R5663 |
T11769 |
T11767 |
appos |
CNS,tissue |
| R5664 |
T11770 |
T11753 |
punct |
),cells |
| R5665 |
T11771 |
T11753 |
prep |
from,cells |
| R5666 |
T11772 |
T11773 |
amod |
adult,mice |
| R5667 |
T11773 |
T11771 |
pobj |
mice,from |
| R5668 |
T11774 |
T11749 |
punct |
.,expressed |
| R5669 |
T11776 |
T11777 |
nsubjpass |
Proteins,resolved |
| R5670 |
T11778 |
T11776 |
prep |
from,Proteins |
| R5671 |
T11779 |
T11780 |
compound |
neuro,2a |
| R5672 |
T11780 |
T11778 |
pobj |
2a,from |
| R5673 |
T11781 |
T11780 |
punct |
-,2a |
| R5674 |
T11782 |
T11780 |
cc |
and,2a |
| R5675 |
T11783 |
T11780 |
conj |
ES,2a |
| R5676 |
T11784 |
T11780 |
appos |
cells,2a |
| R5677 |
T11785 |
T11784 |
punct |
", ",cells |
| R5678 |
T11786 |
T11784 |
conj |
heart,cells |
| R5679 |
T11787 |
T11786 |
cc |
and,heart |
| R5680 |
T11788 |
T11786 |
conj |
brain,heart |
| R5681 |
T11789 |
T11777 |
auxpass |
were,resolved |
| R5682 |
T11790 |
T11777 |
prep |
by,resolved |
| R5683 |
T11791 |
T11792 |
compound |
SDS,electrophoresis |
| R5684 |
T11792 |
T11790 |
pobj |
electrophoresis,by |
| R5685 |
T11793 |
T11794 |
compound |
polyacrylamide,gel |
| R5686 |
T11794 |
T11792 |
compound |
gel,electrophoresis |
| R5687 |
T11795 |
T11796 |
punct |
(,acrylamide |
| R5688 |
T11796 |
T11777 |
parataxis |
acrylamide,resolved |
| R5689 |
T11797 |
T11798 |
nummod |
12,% |
| R5690 |
T11798 |
T11796 |
compound |
%,acrylamide |
| R5691 |
T11799 |
T11796 |
punct |
),acrylamide |
| R5692 |
T11800 |
T11777 |
cc |
and,resolved |
| R5693 |
T11801 |
T11777 |
conj |
transferred,resolved |
| R5694 |
T11802 |
T11801 |
prep |
to,transferred |
| R5695 |
T11803 |
T11804 |
compound |
nitrocellulose,membranes |
| R5696 |
T11804 |
T11802 |
pobj |
membranes,to |
| R5697 |
T11805 |
T11777 |
punct |
.,resolved |
| R5698 |
T11807 |
T11808 |
det |
The,blot |
| R5699 |
T11808 |
T11810 |
nsubjpass |
blot,probed |
| R5700 |
T11809 |
T11808 |
compound |
Western,blot |
| R5701 |
T11811 |
T11810 |
auxpass |
was,probed |
| R5702 |
T11812 |
T11810 |
prep |
with,probed |
| R5703 |
T11813 |
T11814 |
nmod |
mAb,203 |
| R5704 |
T11814 |
T11812 |
pobj |
203,with |
| R5705 |
T11815 |
T11816 |
punct |
–,217 |
| R5706 |
T11816 |
T11814 |
prep |
217,203 |
| R5707 |
T11817 |
T11814 |
acl |
followed,203 |
| R5708 |
T11818 |
T11817 |
agent |
by,followed |
| R5709 |
T11819 |
T11818 |
pobj |
incubation,by |
| R5710 |
T11820 |
T11819 |
prep |
with,incubation |
| R5711 |
T11821 |
T11822 |
det |
a,antibody |
| R5712 |
T11822 |
T11820 |
pobj |
antibody,with |
| R5713 |
T11823 |
T11824 |
npadvmod |
peroxidase,coupled |
| R5714 |
T11824 |
T11822 |
amod |
coupled,antibody |
| R5715 |
T11825 |
T11822 |
amod |
secondary,antibody |
| R5716 |
T11826 |
T11810 |
punct |
.,probed |
| R5717 |
T11828 |
T11829 |
nsubj |
Detection,was |
| R5718 |
T11830 |
T11829 |
prep |
with,was |
| R5719 |
T11831 |
T11832 |
amod |
enhanced,chemiluminescence |
| R5720 |
T11832 |
T11830 |
pobj |
chemiluminescence,with |
| R5721 |
T11833 |
T11829 |
punct |
.,was |
