PMC:1087847 / 4467-22347 JSON TXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T2289	9-16	NN	denotes	Annexin
T2290	17-19	NN	denotes	A7
T2292	20-22	VBZ	denotes	is
T2291	23-32	VBN	denotes	expressed
T2293	33-35	IN	denotes	in
T2294	36-39	DT	denotes	the
T2296	40-45	JJ	denotes	early
T2297	46-51	NN	denotes	mouse
T2295	52-58	NN	denotes	embryo
T2298	58-339	sentence	denotes	First we examined the expression of Annexin A7 in ES cells (Bruce4, established from C57BL/6J mice) and the early stages of mouse embryonic development at the mRNA level by northern blot analysis and at the protein level by Western blotting and immunohistochemistry, respectively.
T2299	59-64	RB	denotes	First
T2301	65-67	PRP	denotes	we
T2300	68-76	VBD	denotes	examined
T2302	77-80	DT	denotes	the
T2303	81-91	NN	denotes	expression
T2304	92-94	IN	denotes	of
T2305	95-102	NN	denotes	Annexin
T2306	103-105	NN	denotes	A7
T2307	106-108	IN	denotes	in
T2308	109-111	NN	denotes	ES
T2309	112-117	NNS	denotes	cells
T2310	118-119	-LRB-	denotes	(
T2311	119-125	NN	denotes	Bruce4
T2312	125-127	,	denotes	,
T2313	127-138	VBN	denotes	established
T2314	139-143	IN	denotes	from
T2315	144-149	NN	denotes	C57BL
T2317	149-150	HYPH	denotes	/
T2316	150-152	NN	denotes	6J
T2318	153-157	NNS	denotes	mice
T2319	157-158	-RRB-	denotes	)
T2320	159-162	CC	denotes	and
T2321	163-166	DT	denotes	the
T2323	167-172	JJ	denotes	early
T2322	173-179	NNS	denotes	stages
T2324	180-182	IN	denotes	of
T2325	183-188	NN	denotes	mouse
T2327	189-198	JJ	denotes	embryonic
T2326	199-210	NN	denotes	development
T2328	211-213	IN	denotes	at
T2329	214-217	DT	denotes	the
T2331	218-222	NN	denotes	mRNA
T2330	223-228	NN	denotes	level
T2332	229-231	IN	denotes	by
T2333	232-240	NNP	denotes	northern
T2334	241-245	NN	denotes	blot
T2335	246-254	NN	denotes	analysis
T2336	255-258	CC	denotes	and
T2337	259-261	IN	denotes	at
T2338	262-265	DT	denotes	the
T2340	266-273	NN	denotes	protein
T2339	274-279	NN	denotes	level
T2341	280-282	IN	denotes	by
T2342	283-290	NNP	denotes	Western
T2343	291-299	NN	denotes	blotting
T2344	300-303	CC	denotes	and
T2345	304-324	NN	denotes	immunohistochemistry
T2346	324-326	,	denotes	,
T2347	326-338	RB	denotes	respectively
T2348	338-339	.	denotes	.
T2349	339-544	sentence	denotes	Northern blot analysis shows in ES-cells and at embryonic days E7, E11, E15, and E17 two mRNA species of 1.8 kb and 2.4 kb, which result from alternative splicing in the untranslated 3'end (Fig. 1A) [11].
T2350	340-348	NNP	denotes	Northern
T2351	349-353	NN	denotes	blot
T2352	354-362	NN	denotes	analysis
T2353	363-368	VBZ	denotes	shows
T2354	369-371	IN	denotes	in
T2355	372-374	NN	denotes	ES
T2357	374-375	HYPH	denotes	-
T2356	375-380	NNS	denotes	cells
T2358	381-384	CC	denotes	and
T2359	385-387	IN	denotes	at
T2360	388-397	JJ	denotes	embryonic
T2362	398-402	NNS	denotes	days
T2361	403-405	NN	denotes	E7
T2363	405-407	,	denotes	,
T2364	407-410	NN	denotes	E11
T2365	410-412	,	denotes	,
T2366	412-415	NN	denotes	E15
T2367	415-417	,	denotes	,
T2368	417-420	CC	denotes	and
T2369	421-424	NN	denotes	E17
T2370	425-428	CD	denotes	two
T2372	429-433	NN	denotes	mRNA
T2371	434-441	NNS	denotes	species
T2373	442-444	IN	denotes	of
T2374	445-448	CD	denotes	1.8
T2375	449-451	NN	denotes	kb
T2376	452-455	CC	denotes	and
T2377	456-459	CD	denotes	2.4
T2378	460-462	NN	denotes	kb
T2379	462-464	,	denotes	,
T2380	464-469	WDT	denotes	which
T2381	470-476	VBP	denotes	result
T2382	477-481	IN	denotes	from
T2383	482-493	JJ	denotes	alternative
T2384	494-502	NN	denotes	splicing
T2385	503-505	IN	denotes	in
T2386	506-509	DT	denotes	the
T2388	510-522	JJ	denotes	untranslated
T2389	523-524	CD	denotes	3
T2390	524-525	SYM	denotes	'
T2387	525-528	NN	denotes	end
T2391	529-530	-LRB-	denotes	(
T2392	530-534	NN	denotes	Fig.
T2393	535-537	CD	denotes	1A
T2394	537-538	-RRB-	denotes	)
T2395	539-540	-LRB-	denotes	[
T2396	540-542	CD	denotes	11
T2397	542-543	-RRB-	denotes	]
T2398	543-544	.	denotes	.
T2399	544-614	sentence	denotes	We found similar Annexin A7 mRNA levels in the four embryonic stages.
T2400	545-547	PRP	denotes	We
T2401	548-553	VBD	denotes	found
T2402	554-561	JJ	denotes	similar
T2404	562-569	NN	denotes	Annexin
T2405	570-572	NN	denotes	A7
T2406	573-577	NN	denotes	mRNA
T2403	578-584	NNS	denotes	levels
T2407	585-587	IN	denotes	in
T2408	588-591	DT	denotes	the
T2410	592-596	CD	denotes	four
T2411	597-606	JJ	denotes	embryonic
T2409	607-613	NNS	denotes	stages
T2412	613-614	.	denotes	.
T2413	614-789	sentence	denotes	Reprobing with a β-actin probe verified equal loading; the appearance of a faster migrating mRNA species in addition to the 2.0 kb species is characteristic for β-actin [12].
T2414	615-624	VBG	denotes	Reprobing
T2416	625-629	IN	denotes	with
T2417	630-631	DT	denotes	a
T2419	632-633	NN	denotes	β
T2421	633-634	HYPH	denotes	-
T2420	634-639	NN	denotes	actin
T2418	640-645	NN	denotes	probe
T2415	646-654	VBD	denotes	verified
T2423	655-660	JJ	denotes	equal
T2424	661-668	NN	denotes	loading
T2425	668-669	:	denotes	;
T2426	670-673	DT	denotes	the
T2427	674-684	NN	denotes	appearance
T2428	685-687	IN	denotes	of
T2429	688-689	DT	denotes	a
T2431	690-696	JJR	denotes	faster
T2432	697-706	VBG	denotes	migrating
T2433	707-711	NN	denotes	mRNA
T2430	712-719	NNS	denotes	species
T2434	720-722	IN	denotes	in
T2435	723-731	NN	denotes	addition
T2436	732-734	IN	denotes	to
T2437	735-738	DT	denotes	the
T2439	739-742	CD	denotes	2.0
T2440	743-745	NN	denotes	kb
T2438	746-753	NNS	denotes	species
T2422	754-756	VBZ	denotes	is
T2441	757-771	JJ	denotes	characteristic
T2442	772-775	IN	denotes	for
T2443	776-777	NN	denotes	β
T2445	777-778	HYPH	denotes	-
T2444	778-783	NN	denotes	actin
T2446	784-785	-LRB-	denotes	[
T2447	785-787	CD	denotes	12
T2448	787-788	-RRB-	denotes	]
T2449	788-789	.	denotes	.
T2450	789-943	sentence	denotes	On the protein level, ES cells express only the smaller Annexin A7 isoform of 47 kDa (Fig. 1B, neuro-2a cells and heart tissue are included for control).
T2451	790-792	IN	denotes	On
T2453	793-796	DT	denotes	the
T2455	797-804	NN	denotes	protein
T2454	805-810	NN	denotes	level
T2456	810-812	,	denotes	,
T2457	812-814	NN	denotes	ES
T2458	815-820	NNS	denotes	cells
T2452	821-828	VBP	denotes	express
T2459	829-833	RB	denotes	only
T2461	834-837	DT	denotes	the
T2462	838-845	JJR	denotes	smaller
T2463	846-853	NN	denotes	Annexin
T2464	854-856	NN	denotes	A7
T2460	857-864	NN	denotes	isoform
T2465	865-867	IN	denotes	of
T2466	868-870	CD	denotes	47
T2467	871-874	NN	denotes	kDa
T2468	875-876	-LRB-	denotes	(
T2470	876-880	NN	denotes	Fig.
T2469	881-883	NN	denotes	1B
T2471	883-885	,	denotes	,
T2472	885-890	NN	denotes	neuro
T2474	890-891	HYPH	denotes	-
T2475	891-893	CD	denotes	2a
T2473	894-899	NNS	denotes	cells
T2477	900-903	CC	denotes	and
T2478	904-909	NN	denotes	heart
T2479	910-916	NN	denotes	tissue
T2480	917-920	VBP	denotes	are
T2476	921-929	VBN	denotes	included
T2481	930-933	IN	denotes	for
T2482	934-941	NN	denotes	control
T2483	941-942	-RRB-	denotes	)
T2484	942-943	.	denotes	.
T2485	943-1867	sentence	denotes	Figure 1 Expression of Annexin A7. (A) Northern blot analysis of RNA from ES-cells and embryos E7, E11, E15, E17 probed with a full length annexin A7 cDNA. Transcripts of 1.8 and 2.4 kb are detected in all stages examined (upper panel). Reprobing with a β-actin probe verified equal loading (lower panel, 2.0 kb band). (B) Murine ES cells (ES) express only the small Annexin A7 isoform. Both isoforms are expressed in neuroblastoma neuro-2a cells (N2A), heart (HRT) and brain tissue (CNS) from adult mice. Proteins from neuro-2a and ES cells, heart and brain were resolved by SDS polyacrylamide gel electrophoresis (12% acrylamide) and transferred to nitrocellulose membranes. The Western blot was probed with mAb 203–217 followed by incubation with a peroxidase coupled secondary antibody. Detection was with enhanced chemiluminescence. Immunohistochemistry confirmed the presence of the protein during early development.
T11654	954-964	NN	denotes	Expression
T11655	965-967	IN	denotes	of
T11656	968-975	NN	denotes	Annexin
T11657	976-978	NN	denotes	A7
T11658	978-979	.	denotes	.
T11659	979-1100	sentence	denotes	(A) Northern blot analysis of RNA from ES-cells and embryos E7, E11, E15, E17 probed with a full length annexin A7 cDNA.
T11660	980-981	-LRB-	denotes	(
T11661	981-982	LS	denotes	A
T11663	982-983	-RRB-	denotes	)
T11664	984-992	NNP	denotes	Northern
T11665	993-997	NN	denotes	blot
T11662	998-1006	NN	denotes	analysis
T11666	1007-1009	IN	denotes	of
T11667	1010-1013	NN	denotes	RNA
T11668	1014-1018	IN	denotes	from
T11669	1019-1021	NN	denotes	ES
T11671	1021-1022	HYPH	denotes	-
T11670	1022-1027	NNS	denotes	cells
T11672	1028-1031	CC	denotes	and
T11673	1032-1039	NNS	denotes	embryos
T11675	1040-1042	NN	denotes	E7
T11676	1042-1044	,	denotes	,
T11677	1044-1047	NN	denotes	E11
T11678	1047-1049	,	denotes	,
T11679	1049-1052	NN	denotes	E15
T11680	1052-1054	,	denotes	,
T11674	1054-1057	NN	denotes	E17
T11681	1058-1064	VBD	denotes	probed
T11682	1065-1069	IN	denotes	with
T11683	1070-1071	DT	denotes	a
T11685	1072-1076	JJ	denotes	full
T11686	1077-1083	NN	denotes	length
T11687	1084-1091	NN	denotes	annexin
T11688	1092-1094	NN	denotes	A7
T11684	1095-1099	NN	denotes	cDNA
T11689	1099-1100	.	denotes	.
T11690	1100-1181	sentence	denotes	Transcripts of 1.8 and 2.4 kb are detected in all stages examined (upper panel).
T11691	1101-1112	NNS	denotes	Transcripts
T11693	1113-1115	IN	denotes	of
T11694	1116-1119	CD	denotes	1.8
T11696	1120-1123	CC	denotes	and
T11697	1124-1127	CD	denotes	2.4
T11695	1128-1130	NN	denotes	kb
T11698	1131-1134	VBP	denotes	are
T11692	1135-1143	VBN	denotes	detected
T11699	1144-1146	IN	denotes	in
T11700	1147-1150	DT	denotes	all
T11701	1151-1157	NNS	denotes	stages
T11702	1158-1166	VBN	denotes	examined
T11703	1167-1168	-LRB-	denotes	(
T11705	1168-1173	JJ	denotes	upper
T11704	1174-1179	NN	denotes	panel
T11706	1179-1180	-RRB-	denotes	)
T11707	1180-1181	.	denotes	.
T11708	1181-1263	sentence	denotes	Reprobing with a β-actin probe verified equal loading (lower panel, 2.0 kb band).
T11709	1182-1191	VBG	denotes	Reprobing
T11711	1192-1196	IN	denotes	with
T11712	1197-1198	DT	denotes	a
T11714	1199-1200	NN	denotes	β
T11716	1200-1201	HYPH	denotes	-
T11715	1201-1206	NN	denotes	actin
T11713	1207-1212	NN	denotes	probe
T11710	1213-1221	VBD	denotes	verified
T11717	1222-1227	JJ	denotes	equal
T11718	1228-1235	NN	denotes	loading
T11719	1236-1237	-LRB-	denotes	(
T11721	1237-1242	JJ	denotes	lower
T11722	1243-1248	NN	denotes	panel
T11723	1248-1250	,	denotes	,
T11724	1250-1253	CD	denotes	2.0
T11725	1254-1256	NN	denotes	kb
T11720	1257-1261	NN	denotes	band
T11726	1261-1262	-RRB-	denotes	)
T11727	1262-1263	.	denotes	.
T11728	1263-1331	sentence	denotes	(B) Murine ES cells (ES) express only the small Annexin A7 isoform.
T11729	1264-1265	-LRB-	denotes	(
T11730	1265-1266	LS	denotes	B
T11732	1266-1267	-RRB-	denotes	)
T11733	1268-1274	JJ	denotes	Murine
T11735	1275-1277	NN	denotes	ES
T11734	1278-1283	NNS	denotes	cells
T11736	1284-1285	-LRB-	denotes	(
T11737	1285-1287	NN	denotes	ES
T11738	1287-1288	-RRB-	denotes	)
T11731	1289-1296	VBP	denotes	express
T11739	1297-1301	RB	denotes	only
T11741	1302-1305	DT	denotes	the
T11742	1306-1311	JJ	denotes	small
T11743	1312-1319	NN	denotes	Annexin
T11744	1320-1322	NN	denotes	A7
T11740	1323-1330	NN	denotes	isoform
T11745	1330-1331	.	denotes	.
T11746	1331-1450	sentence	denotes	Both isoforms are expressed in neuroblastoma neuro-2a cells (N2A), heart (HRT) and brain tissue (CNS) from adult mice.
T11747	1332-1336	DT	denotes	Both
T11748	1337-1345	NNS	denotes	isoforms
T11750	1346-1349	VBP	denotes	are
T11749	1350-1359	VBN	denotes	expressed
T11751	1360-1362	IN	denotes	in
T11752	1363-1376	NN	denotes	neuroblastoma
T11754	1377-1382	NN	denotes	neuro
T11756	1382-1383	HYPH	denotes	-
T11755	1383-1385	NN	denotes	2a
T11753	1386-1391	NNS	denotes	cells
T11757	1392-1393	-LRB-	denotes	(
T11758	1393-1396	NN	denotes	N2A
T11759	1396-1397	-RRB-	denotes	)
T11760	1397-1399	,	denotes	,
T11761	1399-1404	NN	denotes	heart
T11762	1405-1406	-LRB-	denotes	(
T11763	1406-1409	NN	denotes	HRT
T11764	1409-1410	-RRB-	denotes	)
T11765	1411-1414	CC	denotes	and
T11766	1415-1420	NN	denotes	brain
T11767	1421-1427	NN	denotes	tissue
T11768	1428-1429	-LRB-	denotes	(
T11769	1429-1432	NN	denotes	CNS
T11770	1432-1433	-RRB-	denotes	)
T11771	1434-1438	IN	denotes	from
T11772	1439-1444	JJ	denotes	adult
T11773	1445-1449	NNS	denotes	mice
T11774	1449-1450	.	denotes	.
T11775	1450-1621	sentence	denotes	Proteins from neuro-2a and ES cells, heart and brain were resolved by SDS polyacrylamide gel electrophoresis (12% acrylamide) and transferred to nitrocellulose membranes.
T11776	1451-1459	NNS	denotes	Proteins
T11778	1460-1464	IN	denotes	from
T11779	1465-1470	NN	denotes	neuro
T11781	1470-1471	HYPH	denotes	-
T11780	1471-1473	NN	denotes	2a
T11782	1474-1477	CC	denotes	and
T11783	1478-1480	NN	denotes	ES
T11784	1481-1486	NNS	denotes	cells
T11785	1486-1488	,	denotes	,
T11786	1488-1493	NN	denotes	heart
T11787	1494-1497	CC	denotes	and
T11788	1498-1503	NN	denotes	brain
T11789	1504-1508	VBD	denotes	were
T11777	1509-1517	VBN	denotes	resolved
T11790	1518-1520	IN	denotes	by
T11791	1521-1524	NN	denotes	SDS
T11793	1525-1539	NN	denotes	polyacrylamide
T11794	1540-1543	NN	denotes	gel
T11792	1544-1559	NN	denotes	electrophoresis
T11795	1560-1561	-LRB-	denotes	(
T11797	1561-1563	CD	denotes	12
T11798	1563-1564	NN	denotes	%
T11796	1565-1575	NN	denotes	acrylamide
T11799	1575-1576	-RRB-	denotes	)
T11800	1577-1580	CC	denotes	and
T11801	1581-1592	VBN	denotes	transferred
T11802	1593-1595	IN	denotes	to
T11803	1596-1610	NN	denotes	nitrocellulose
T11804	1611-1620	NNS	denotes	membranes
T11805	1620-1621	.	denotes	.
T11806	1621-1735	sentence	denotes	The Western blot was probed with mAb 203–217 followed by incubation with a peroxidase coupled secondary antibody.
T11807	1622-1625	DT	denotes	The
T11809	1626-1633	NNP	denotes	Western
T11808	1634-1638	NN	denotes	blot
T11811	1639-1642	VBD	denotes	was
T11810	1643-1649	VBN	denotes	probed
T11812	1650-1654	IN	denotes	with
T11813	1655-1658	NN	denotes	mAb
T11814	1659-1662	CD	denotes	203
T11815	1662-1663	SYM	denotes	–
T11816	1663-1666	CD	denotes	217
T11817	1667-1675	VBN	denotes	followed
T11818	1676-1678	IN	denotes	by
T11819	1679-1689	NN	denotes	incubation
T11820	1690-1694	IN	denotes	with
T11821	1695-1696	DT	denotes	a
T11823	1697-1707	NN	denotes	peroxidase
T11824	1708-1715	VBN	denotes	coupled
T11825	1716-1725	JJ	denotes	secondary
T11822	1726-1734	NN	denotes	antibody
T11826	1734-1735	.	denotes	.
T11827	1735-1782	sentence	denotes	Detection was with enhanced chemiluminescence.
T11828	1736-1745	NN	denotes	Detection
T11829	1746-1749	VBD	denotes	was
T11830	1750-1754	IN	denotes	with
T11831	1755-1763	VBN	denotes	enhanced
T11832	1764-1781	NN	denotes	chemiluminescence
T11833	1781-1782	.	denotes	.
T2486	1783-1803	NN	denotes	Immunohistochemistry
T2487	1804-1813	VBD	denotes	confirmed
T2488	1814-1817	DT	denotes	the
T2489	1818-1826	NN	denotes	presence
T2490	1827-1829	IN	denotes	of
T2491	1830-1833	DT	denotes	the
T2492	1834-1841	NN	denotes	protein
T2493	1842-1848	IN	denotes	during
T2494	1849-1854	JJ	denotes	early
T2495	1855-1866	NN	denotes	development
T2496	1866-1867	.	denotes	.
T2497	1867-2104	sentence	denotes	Annexin A7 immunoreactivity using mAb 203–217, which specifically recognizes both isoforms of Annexin A7, can be observed in the two cell types of the cylinder stage at E5 with a weaker Annexin A7 expression in the ectoderm (Fig. 2A–C).
T2498	1868-1875	NN	denotes	Annexin
T2499	1876-1878	NN	denotes	A7
T2500	1879-1895	NN	denotes	immunoreactivity
T2502	1896-1901	VBG	denotes	using
T2503	1902-1905	NN	denotes	mAb
T2504	1906-1909	CD	denotes	203
T2505	1909-1910	SYM	denotes	–
T2506	1910-1913	CD	denotes	217
T2507	1913-1915	,	denotes	,
T2508	1915-1920	WDT	denotes	which
T2510	1921-1933	RB	denotes	specifically
T2509	1934-1944	VBZ	denotes	recognizes
T2511	1945-1949	DT	denotes	both
T2512	1950-1958	NNS	denotes	isoforms
T2513	1959-1961	IN	denotes	of
T2514	1962-1969	NN	denotes	Annexin
T2515	1970-1972	NN	denotes	A7
T2516	1972-1974	,	denotes	,
T2517	1974-1977	MD	denotes	can
T2518	1978-1980	VB	denotes	be
T2501	1981-1989	VBN	denotes	observed
T2519	1990-1992	IN	denotes	in
T2520	1993-1996	DT	denotes	the
T2522	1997-2000	CD	denotes	two
T2523	2001-2005	NN	denotes	cell
T2521	2006-2011	NNS	denotes	types
T2524	2012-2014	IN	denotes	of
T2525	2015-2018	DT	denotes	the
T2527	2019-2027	NN	denotes	cylinder
T2526	2028-2033	NN	denotes	stage
T2528	2034-2036	IN	denotes	at
T2529	2037-2039	NN	denotes	E5
T2530	2040-2044	IN	denotes	with
T2531	2045-2046	DT	denotes	a
T2533	2047-2053	JJR	denotes	weaker
T2534	2054-2061	NN	denotes	Annexin
T2535	2062-2064	NN	denotes	A7
T2532	2065-2075	NN	denotes	expression
T2536	2076-2078	IN	denotes	in
T2537	2079-2082	DT	denotes	the
T2538	2083-2091	NN	denotes	ectoderm
T2539	2092-2093	-LRB-	denotes	(
T2541	2093-2097	NN	denotes	Fig.
T2540	2098-2100	NN	denotes	2A
T2542	2100-2101	SYM	denotes	–
T2543	2101-2102	NN	denotes	C
T2544	2102-2103	-RRB-	denotes	)
T2545	2103-2104	.	denotes	.
T2546	2104-2191	sentence	denotes	At E8 Annexin A7 can be detected in almost all tissues of the embryo (data not shown).
T2547	2105-2107	IN	denotes	At
T2549	2108-2110	NN	denotes	E8
T2550	2111-2118	NN	denotes	Annexin
T2551	2119-2121	NN	denotes	A7
T2552	2122-2125	MD	denotes	can
T2553	2126-2128	VB	denotes	be
T2548	2129-2137	VBN	denotes	detected
T2554	2138-2140	IN	denotes	in
T2555	2141-2147	RB	denotes	almost
T2557	2148-2151	DT	denotes	all
T2556	2152-2159	NNS	denotes	tissues
T2558	2160-2162	IN	denotes	of
T2559	2163-2166	DT	denotes	the
T2560	2167-2173	NN	denotes	embryo
T2561	2174-2175	-LRB-	denotes	(
T2563	2175-2179	NNS	denotes	data
T2564	2180-2183	RB	denotes	not
T2562	2184-2189	VBN	denotes	shown
T2565	2189-2190	-RRB-	denotes	)
T2566	2190-2191	.	denotes	.
T2567	2191-2321	sentence	denotes	A correlation between the presence of Annexin A7 and the origin of a cell type from one of the three germ layers is not apparent.
T2568	2192-2193	DT	denotes	A
T2569	2194-2205	NN	denotes	correlation
T2571	2206-2213	IN	denotes	between
T2572	2214-2217	DT	denotes	the
T2573	2218-2226	NN	denotes	presence
T2574	2227-2229	IN	denotes	of
T2575	2230-2237	NN	denotes	Annexin
T2576	2238-2240	NN	denotes	A7
T2577	2241-2244	CC	denotes	and
T2578	2245-2248	DT	denotes	the
T2579	2249-2255	NN	denotes	origin
T2580	2256-2258	IN	denotes	of
T2581	2259-2260	DT	denotes	a
T2583	2261-2265	NN	denotes	cell
T2582	2266-2270	NN	denotes	type
T2584	2271-2275	IN	denotes	from
T2585	2276-2279	CD	denotes	one
T2587	2280-2282	IN	denotes	of
T2588	2283-2286	DT	denotes	the
T2586	2287-2292	CD	denotes	three
T2590	2293-2297	NN	denotes	germ
T2589	2298-2304	NNS	denotes	layers
T2570	2305-2307	VBZ	denotes	is
T2591	2308-2311	RB	denotes	not
T2592	2312-2320	JJ	denotes	apparent
T2593	2320-2321	.	denotes	.
T2594	2321-2477	sentence	denotes	We specifically noted the localization of the protein in the neuroepithelium of the neural fold and tube (Fig. 2D–F, proximal, E8; Fig. 2G–I, distal, E13).
T2595	2322-2324	PRP	denotes	We
T2597	2325-2337	RB	denotes	specifically
T2596	2338-2343	VBD	denotes	noted
T2598	2344-2347	DT	denotes	the
T2599	2348-2360	NN	denotes	localization
T2600	2361-2363	IN	denotes	of
T2601	2364-2367	DT	denotes	the
T2602	2368-2375	NN	denotes	protein
T2603	2376-2378	IN	denotes	in
T2604	2379-2382	DT	denotes	the
T2605	2383-2398	NN	denotes	neuroepithelium
T2606	2399-2401	IN	denotes	of
T2607	2402-2405	DT	denotes	the
T2609	2406-2412	JJ	denotes	neural
T2608	2413-2417	NN	denotes	fold
T2610	2418-2421	CC	denotes	and
T2611	2422-2426	NN	denotes	tube
T2612	2427-2428	-LRB-	denotes	(
T2614	2428-2432	NN	denotes	Fig.
T2615	2433-2435	NN	denotes	2D
T2617	2435-2436	SYM	denotes	–
T2618	2436-2437	NN	denotes	F
T2619	2437-2439	,	denotes	,
T2620	2439-2447	JJ	denotes	proximal
T2621	2447-2449	,	denotes	,
T2616	2449-2451	NN	denotes	E8
T2622	2451-2452	:	denotes	;
T2623	2453-2457	NN	denotes	Fig.
T2624	2458-2460	NN	denotes	2G
T2625	2460-2461	SYM	denotes	–
T2626	2461-2462	NN	denotes	I
T2627	2462-2464	,	denotes	,
T2628	2464-2470	JJ	denotes	distal
T2629	2470-2472	,	denotes	,
T2613	2472-2475	NN	denotes	E13
T2630	2475-2476	-RRB-	denotes	)
T2631	2476-2477	.	denotes	.
T2632	2477-2606	sentence	denotes	At the subcellular level, Annexin A7 was present in punctate structures mainly in the cytosol of all examined cells (Fig. 2E,F).
T2633	2478-2480	IN	denotes	At
T2635	2481-2484	DT	denotes	the
T2637	2485-2496	JJ	denotes	subcellular
T2636	2497-2502	NN	denotes	level
T2638	2502-2504	,	denotes	,
T2639	2504-2511	NN	denotes	Annexin
T2640	2512-2514	NN	denotes	A7
T2634	2515-2518	VBD	denotes	was
T2641	2519-2526	JJ	denotes	present
T2642	2527-2529	IN	denotes	in
T2643	2530-2538	NN	denotes	punctate
T2644	2539-2549	NNS	denotes	structures
T2645	2550-2556	RB	denotes	mainly
T2646	2557-2559	IN	denotes	in
T2647	2560-2563	DT	denotes	the
T2648	2564-2571	NN	denotes	cytosol
T2649	2572-2574	IN	denotes	of
T2650	2575-2578	DT	denotes	all
T2652	2579-2587	VBN	denotes	examined
T2651	2588-2593	NNS	denotes	cells
T2653	2594-2595	-LRB-	denotes	(
T2655	2595-2599	NN	denotes	Fig.
T2656	2600-2602	NN	denotes	2E
T2657	2602-2603	,	denotes	,
T2654	2603-2604	NN	denotes	F
T2658	2604-2605	-RRB-	denotes	)
T2659	2605-2606	.	denotes	.
T2660	2606-2673	sentence	denotes	This distribution was also observed during subsequent development.
T2661	2607-2611	DT	denotes	This
T2662	2612-2624	NN	denotes	distribution
T2664	2625-2628	VBD	denotes	was
T2665	2629-2633	RB	denotes	also
T2663	2634-2642	VBN	denotes	observed
T2666	2643-2649	IN	denotes	during
T2667	2650-2660	JJ	denotes	subsequent
T2668	2661-2672	NN	denotes	development
T2669	2672-2673	.	denotes	.
T12193	2684-2691	NN	denotes	Annexin
T12194	2692-2694	NN	denotes	A7
T12195	2695-2711	NN	denotes	immunoreactivity
T12196	2712-2714	IN	denotes	in
T12197	2715-2720	JJ	denotes	early
T12199	2721-2726	NN	denotes	mouse
T12198	2727-2734	NNS	denotes	embryos
T12200	2734-2735	.	denotes	.
T12201	2735-2886	sentence	denotes	(A) Phase contrast, embryo E5: The egg cylinder consists of an inner cell mass (a) representing the ectoderm and an outer layer of endoderm cells (b).
T12202	2736-2737	-LRB-	denotes	(
T12203	2737-2738	LS	denotes	A
T12205	2738-2739	-RRB-	denotes	)
T12206	2740-2745	NN	denotes	Phase
T12207	2746-2754	NN	denotes	contrast
T12208	2754-2756	,	denotes	,
T12209	2756-2762	NN	denotes	embryo
T12210	2763-2765	NN	denotes	E5
T12211	2765-2767	:	denotes	:
T12212	2767-2770	DT	denotes	The
T12214	2771-2774	NN	denotes	egg
T12213	2775-2783	NN	denotes	cylinder
T12204	2784-2792	VBZ	denotes	consists
T12215	2793-2795	IN	denotes	of
T12216	2796-2798	DT	denotes	an
T12218	2799-2804	JJ	denotes	inner
T12219	2805-2809	NN	denotes	cell
T12217	2810-2814	NN	denotes	mass
T12220	2815-2816	-LRB-	denotes	(
T12221	2816-2817	NN	denotes	a
T12222	2817-2818	-RRB-	denotes	)
T12223	2819-2831	VBG	denotes	representing
T12224	2832-2835	DT	denotes	the
T12225	2836-2844	NN	denotes	ectoderm
T12226	2845-2848	CC	denotes	and
T12227	2849-2851	DT	denotes	an
T12229	2852-2857	JJ	denotes	outer
T12228	2858-2863	NN	denotes	layer
T12230	2864-2866	IN	denotes	of
T12231	2867-2875	NN	denotes	endoderm
T12232	2876-2881	NNS	denotes	cells
T12233	2882-2883	-LRB-	denotes	(
T12234	2883-2884	NN	denotes	b
T12235	2884-2885	-RRB-	denotes	)
T12236	2885-2886	.	denotes	.
T12237	2886-3005	sentence	denotes	(B) Immunostaining of the paraffin section was performed using purified mAb 203–217 and Cy3-conjugated anti-mouse IgG.
T12238	2887-2888	-LRB-	denotes	(
T12239	2888-2889	LS	denotes	B
T12241	2889-2890	-RRB-	denotes	)
T12242	2891-2905	NN	denotes	Immunostaining
T12243	2906-2908	IN	denotes	of
T12244	2909-2912	DT	denotes	the
T12246	2913-2921	NN	denotes	paraffin
T12245	2922-2929	NN	denotes	section
T12247	2930-2933	VBD	denotes	was
T12240	2934-2943	VBN	denotes	performed
T12248	2944-2949	VBG	denotes	using
T12249	2950-2958	VBN	denotes	purified
T12250	2959-2962	NN	denotes	mAb
T12251	2963-2966	CD	denotes	203
T12252	2966-2967	SYM	denotes	–
T12253	2967-2970	CD	denotes	217
T12254	2971-2974	CC	denotes	and
T12255	2975-2978	NN	denotes	Cy3
T12257	2978-2979	HYPH	denotes	-
T12256	2979-2989	VBN	denotes	conjugated
T12259	2990-3000	JJ	denotes	anti-mouse
T12258	3001-3004	NN	denotes	IgG
T12260	3004-3005	.	denotes	.
T12261	3005-3146	sentence	denotes	Annexin A7 is expressed in both cell types of the egg cylinder with a strong staining of the endoderm and a weaker staining of the ectoderm.
T12262	3006-3013	NN	denotes	Annexin
T12263	3014-3016	NN	denotes	A7
T12265	3017-3019	VBZ	denotes	is
T12264	3020-3029	VBN	denotes	expressed
T12266	3030-3032	IN	denotes	in
T12267	3033-3037	DT	denotes	both
T12269	3038-3042	NN	denotes	cell
T12268	3043-3048	NNS	denotes	types
T12270	3049-3051	IN	denotes	of
T12271	3052-3055	DT	denotes	the
T12273	3056-3059	NN	denotes	egg
T12272	3060-3068	NN	denotes	cylinder
T12274	3069-3073	IN	denotes	with
T12275	3074-3075	DT	denotes	a
T12277	3076-3082	JJ	denotes	strong
T12276	3083-3091	NN	denotes	staining
T12278	3092-3094	IN	denotes	of
T12279	3095-3098	DT	denotes	the
T12280	3099-3107	NN	denotes	endoderm
T12281	3108-3111	CC	denotes	and
T12282	3112-3113	DT	denotes	a
T12284	3114-3120	JJR	denotes	weaker
T12283	3121-3129	NN	denotes	staining
T12285	3130-3132	IN	denotes	of
T12286	3133-3136	DT	denotes	the
T12287	3137-3145	NN	denotes	ectoderm
T12288	3145-3146	.	denotes	.
T12289	3146-3189	sentence	denotes	The nuclei are devoid of immune reactions.
T12290	3147-3150	DT	denotes	The
T12291	3151-3157	NNS	denotes	nuclei
T12292	3158-3161	VBP	denotes	are
T12293	3162-3168	JJ	denotes	devoid
T12294	3169-3171	IN	denotes	of
T12295	3172-3178	JJ	denotes	immune
T12296	3179-3188	NNS	denotes	reactions
T12297	3188-3189	.	denotes	.
T12298	3189-3249	sentence	denotes	(C) Negative control using the secondary Cy3-antibody only.
T12299	3190-3191	-LRB-	denotes	(
T12301	3191-3192	LS	denotes	C
T12302	3192-3193	-RRB-	denotes	)
T12303	3194-3202	JJ	denotes	Negative
T12300	3203-3210	NN	denotes	control
T12304	3211-3216	VBG	denotes	using
T12305	3217-3220	DT	denotes	the
T12307	3221-3230	JJ	denotes	secondary
T12308	3231-3234	NN	denotes	Cy3
T12309	3234-3235	HYPH	denotes	-
T12306	3235-3243	NN	denotes	antibody
T12310	3244-3248	RB	denotes	only
T12311	3248-3249	.	denotes	.
T12312	3249-3370	sentence	denotes	(D-F) Annexin A7 expression in the proximal neural tube (D) and nearby neural fold (E,F), embryo E8, transverse section.
T12313	3250-3251	-LRB-	denotes	(
T12314	3251-3252	LS	denotes	D
T12316	3252-3253	SYM	denotes	-
T12317	3253-3254	LS	denotes	F
T12318	3254-3255	-RRB-	denotes	)
T12319	3256-3263	NN	denotes	Annexin
T12320	3264-3266	NN	denotes	A7
T12321	3267-3277	NN	denotes	expression
T12322	3278-3280	IN	denotes	in
T12323	3281-3284	DT	denotes	the
T12325	3285-3293	JJ	denotes	proximal
T12326	3294-3300	JJ	denotes	neural
T12324	3301-3305	NN	denotes	tube
T12327	3306-3307	-LRB-	denotes	(
T12328	3307-3308	NN	denotes	D
T12329	3308-3309	-RRB-	denotes	)
T12330	3310-3313	CC	denotes	and
T12331	3314-3320	JJ	denotes	nearby
T12333	3321-3327	JJ	denotes	neural
T12332	3328-3332	NN	denotes	fold
T12334	3333-3334	-LRB-	denotes	(
T12336	3334-3335	NN	denotes	E
T12337	3335-3336	,	denotes	,
T12335	3336-3337	NN	denotes	F
T12338	3337-3338	-RRB-	denotes	)
T12339	3338-3340	,	denotes	,
T12340	3340-3346	NN	denotes	embryo
T12341	3347-3349	NN	denotes	E8
T12342	3349-3351	,	denotes	,
T12343	3351-3361	JJ	denotes	transverse
T12315	3362-3369	NN	denotes	section
T12344	3369-3370	.	denotes	.
T12345	3370-3512	sentence	denotes	Immunolabeling of Annexin A7 was performed with purified mAb 203–217 and visualization was with an Alexa Fluor 488-conjugated anti-mouse IgG.
T12346	3371-3385	NN	denotes	Immunolabeling
T12348	3386-3388	IN	denotes	of
T12349	3389-3396	NN	denotes	Annexin
T12350	3397-3399	NN	denotes	A7
T12351	3400-3403	VBD	denotes	was
T12347	3404-3413	VBN	denotes	performed
T12352	3414-3418	IN	denotes	with
T12353	3419-3427	VBN	denotes	purified
T12355	3428-3431	NN	denotes	mAb
T12354	3432-3435	CD	denotes	203
T12356	3435-3436	SYM	denotes	–
T12357	3436-3439	CD	denotes	217
T12358	3440-3443	CC	denotes	and
T12359	3444-3457	NN	denotes	visualization
T12360	3458-3461	VBD	denotes	was
T12361	3462-3466	IN	denotes	with
T12362	3467-3469	DT	denotes	an
T12364	3470-3475	NNP	denotes	Alexa
T12365	3476-3481	NNP	denotes	Fluor
T12367	3482-3485	CD	denotes	488
T12368	3485-3486	HYPH	denotes	-
T12366	3486-3496	VBN	denotes	conjugated
T12369	3497-3507	JJ	denotes	anti-mouse
T12363	3508-3511	NN	denotes	IgG
T12370	3511-3512	.	denotes	.
T12371	3512-3636	sentence	denotes	(D) An intense Annexin A7 immunostaining is detectable in the neuroepithelium of the neural tube (a, lumen of neural tube).
T12372	3513-3514	-LRB-	denotes	(
T12373	3514-3515	LS	denotes	D
T12375	3515-3516	-RRB-	denotes	)
T12376	3517-3519	DT	denotes	An
T12378	3520-3527	JJ	denotes	intense
T12379	3528-3535	NN	denotes	Annexin
T12380	3536-3538	NN	denotes	A7
T12377	3539-3553	NN	denotes	immunostaining
T12374	3554-3556	VBZ	denotes	is
T12381	3557-3567	JJ	denotes	detectable
T12382	3568-3570	IN	denotes	in
T12383	3571-3574	DT	denotes	the
T12384	3575-3590	NN	denotes	neuroepithelium
T12385	3591-3593	IN	denotes	of
T12386	3594-3597	DT	denotes	the
T12388	3598-3604	JJ	denotes	neural
T12387	3605-3609	NN	denotes	tube
T12389	3610-3611	-LRB-	denotes	(
T12391	3611-3612	NN	denotes	a
T12392	3612-3614	,	denotes	,
T12390	3614-3619	NN	denotes	lumen
T12393	3620-3622	IN	denotes	of
T12394	3623-3629	JJ	denotes	neural
T12395	3630-3634	NN	denotes	tube
T12396	3634-3635	-RRB-	denotes	)
T12397	3635-3636	.	denotes	.
T12398	3636-3737	sentence	denotes	(E,F) Higher magnifications of the neuroepithelium show that Annexin A7 is expressed in the cytosol.
T12399	3637-3638	-LRB-	denotes	(
T12400	3638-3639	LS	denotes	E
T12402	3639-3640	,	denotes	,
T12403	3640-3641	LS	denotes	F
T12404	3641-3642	-RRB-	denotes	)
T12405	3643-3649	JJR	denotes	Higher
T12406	3650-3664	NNS	denotes	magnifications
T12407	3665-3667	IN	denotes	of
T12408	3668-3671	DT	denotes	the
T12409	3672-3687	NN	denotes	neuroepithelium
T12401	3688-3692	VBP	denotes	show
T12410	3693-3697	IN	denotes	that
T12412	3698-3705	NN	denotes	Annexin
T12413	3706-3708	NN	denotes	A7
T12414	3709-3711	VBZ	denotes	is
T12411	3712-3721	VBN	denotes	expressed
T12415	3722-3724	IN	denotes	in
T12416	3725-3728	DT	denotes	the
T12417	3729-3736	NN	denotes	cytosol
T12418	3736-3737	.	denotes	.
T12419	3737-3801	sentence	denotes	Arrowheads point to Annexin A7 immunoreactivity in the cytosol.
T12420	3738-3748	NNS	denotes	Arrowheads
T12421	3749-3754	VBP	denotes	point
T12422	3755-3757	IN	denotes	to
T12423	3758-3765	NN	denotes	Annexin
T12424	3766-3768	NN	denotes	A7
T12425	3769-3785	NN	denotes	immunoreactivity
T12426	3786-3788	IN	denotes	in
T12427	3789-3792	DT	denotes	the
T12428	3793-3800	NN	denotes	cytosol
T12429	3800-3801	.	denotes	.
T12430	3801-3853	sentence	denotes	(G) Phase contrast, embryo E13, caudal neural tube.
T12431	3802-3803	-LRB-	denotes	(
T12433	3803-3804	LS	denotes	G
T12434	3804-3805	-RRB-	denotes	)
T12435	3806-3811	NN	denotes	Phase
T12436	3812-3820	NN	denotes	contrast
T12437	3820-3822	,	denotes	,
T12438	3822-3828	NN	denotes	embryo
T12439	3829-3832	NN	denotes	E13
T12440	3832-3834	,	denotes	,
T12441	3834-3840	JJ	denotes	caudal
T12442	3841-3847	JJ	denotes	neural
T12432	3848-3852	NN	denotes	tube
T12443	3852-3853	.	denotes	.
T12444	3853-3984	sentence	denotes	(H) Immunostaining of the paraffin section was performed using purified mAb 203–217 and Alexa Fluor 488-conjugated anti-mouse IgG.
T12445	3854-3855	-LRB-	denotes	(
T12446	3855-3856	LS	denotes	H
T12448	3856-3857	-RRB-	denotes	)
T12449	3858-3872	NN	denotes	Immunostaining
T12450	3873-3875	IN	denotes	of
T12451	3876-3879	DT	denotes	the
T12453	3880-3888	NN	denotes	paraffin
T12452	3889-3896	NN	denotes	section
T12454	3897-3900	VBD	denotes	was
T12447	3901-3910	VBN	denotes	performed
T12455	3911-3916	VBG	denotes	using
T12456	3917-3925	VBN	denotes	purified
T12458	3926-3929	NN	denotes	mAb
T12457	3930-3933	CD	denotes	203
T12459	3933-3934	SYM	denotes	–
T12460	3934-3937	CD	denotes	217
T12461	3938-3941	CC	denotes	and
T12462	3942-3947	NNP	denotes	Alexa
T12463	3948-3953	NNP	denotes	Fluor
T12465	3954-3957	CD	denotes	488
T12466	3957-3958	HYPH	denotes	-
T12464	3958-3968	VBN	denotes	conjugated
T12468	3969-3979	JJ	denotes	anti-mouse
T12467	3980-3983	NN	denotes	IgG
T12469	3983-3984	.	denotes	.
T12470	3984-4056	sentence	denotes	(I) Negative control using the secondary Alexa Fluor 488-antibody only.
T12471	3985-3986	-LRB-	denotes	(
T12472	3986-3987	LS	denotes	I
T12474	3987-3988	-RRB-	denotes	)
T12475	3989-3997	JJ	denotes	Negative
T12473	3998-4005	NN	denotes	control
T12476	4006-4011	VBG	denotes	using
T12477	4012-4015	DT	denotes	the
T12479	4016-4025	JJ	denotes	secondary
T12480	4026-4031	NNP	denotes	Alexa
T12481	4032-4037	NNP	denotes	Fluor
T12482	4038-4041	CD	denotes	488
T12483	4041-4042	HYPH	denotes	-
T12478	4042-4050	NN	denotes	antibody
T12484	4051-4055	RB	denotes	only
T12485	4055-4056	.	denotes	.
T12486	4056-4068	sentence	denotes	Bar, 20 μm.
T12487	4057-4060	NN	denotes	Bar
T12488	4060-4062	,	denotes	,
T12489	4062-4064	CD	denotes	20
T12490	4065-4067	NN	denotes	μm
T12491	4067-4068	.	denotes	.
T2973	4070-4077	NN	denotes	Annexin
T2974	4078-4080	NN	denotes	A7
T2975	4081-4088	VBZ	denotes	changes
T2976	4089-4092	PRP$	denotes	its
T2978	4093-4104	JJ	denotes	subcellular
T2977	4105-4117	NN	denotes	localization
T2979	4118-4120	IN	denotes	in
T2980	4121-4124	DT	denotes	the
T2982	4125-4134	JJ	denotes	embryonic
T2981	4135-4140	NN	denotes	brain
T2983	4141-4143	IN	denotes	at
T2984	4144-4147	NN	denotes	E16
T2985	4147-4260	sentence	denotes	Next we analysed the cellular and subcellular distribution of Annexin A7 in the developing mouse brain (Fig. 3).
T2986	4148-4152	RB	denotes	Next
T2988	4153-4155	PRP	denotes	we
T2987	4156-4164	VBD	denotes	analysed
T2989	4165-4168	DT	denotes	the
T2991	4169-4177	JJ	denotes	cellular
T2992	4178-4181	CC	denotes	and
T2993	4182-4193	JJ	denotes	subcellular
T2990	4194-4206	NN	denotes	distribution
T2994	4207-4209	IN	denotes	of
T2995	4210-4217	NN	denotes	Annexin
T2996	4218-4220	NN	denotes	A7
T2997	4221-4223	IN	denotes	in
T2998	4224-4227	DT	denotes	the
T3000	4228-4238	VBG	denotes	developing
T3001	4239-4244	NN	denotes	mouse
T2999	4245-4250	NN	denotes	brain
T3002	4251-4252	-LRB-	denotes	(
T3003	4252-4256	NN	denotes	Fig.
T3004	4257-4258	CD	denotes	3
T3005	4258-4259	-RRB-	denotes	)
T3006	4259-4260	.	denotes	.
T3007	4260-4481	sentence	denotes	At E13–E16 in transverse sections of the embryonic brain, most of the immunoreactive cells are present in the ventricular germinative zone surrounding the lateral ventricle (Fig. 3A, overview E16; Fig. 3H, overview E13).
T3008	4261-4263	IN	denotes	At
T3010	4264-4267	NN	denotes	E13
T3011	4267-4268	SYM	denotes	–
T3012	4268-4271	NN	denotes	E16
T3013	4272-4274	IN	denotes	in
T3014	4275-4285	JJ	denotes	transverse
T3015	4286-4294	NNS	denotes	sections
T3016	4295-4297	IN	denotes	of
T3017	4298-4301	DT	denotes	the
T3019	4302-4311	JJ	denotes	embryonic
T3018	4312-4317	NN	denotes	brain
T3020	4317-4319	,	denotes	,
T3021	4319-4323	JJS	denotes	most
T3022	4324-4326	IN	denotes	of
T3023	4327-4330	DT	denotes	the
T3025	4331-4345	JJ	denotes	immunoreactive
T3024	4346-4351	NNS	denotes	cells
T3009	4352-4355	VBP	denotes	are
T3026	4356-4363	JJ	denotes	present
T3027	4364-4366	IN	denotes	in
T3028	4367-4370	DT	denotes	the
T3030	4371-4382	JJ	denotes	ventricular
T3031	4383-4394	JJ	denotes	germinative
T3029	4395-4399	NN	denotes	zone
T3032	4400-4411	VBG	denotes	surrounding
T3033	4412-4415	DT	denotes	the
T3035	4416-4423	JJ	denotes	lateral
T3034	4424-4433	NN	denotes	ventricle
T3036	4434-4435	-LRB-	denotes	(
T3038	4435-4439	NN	denotes	Fig.
T3040	4440-4442	CD	denotes	3A
T3041	4442-4444	,	denotes	,
T3042	4444-4452	NN	denotes	overview
T3039	4453-4456	NN	denotes	E16
T3043	4456-4457	:	denotes	;
T3044	4458-4462	NN	denotes	Fig.
T3045	4463-4465	CD	denotes	3H
T3046	4465-4467	,	denotes	,
T3047	4467-4475	NN	denotes	overview
T3037	4476-4479	NN	denotes	E13
T3048	4479-4480	-RRB-	denotes	)
T3049	4480-4481	.	denotes	.
T3050	4481-4581	sentence	denotes	A closer inspection revealed that Annexin A7 is mainly localized in the cytosol at E13 (Fig. 3B,E).
T3051	4482-4483	DT	denotes	A
T3053	4484-4490	JJR	denotes	closer
T3052	4491-4501	NN	denotes	inspection
T3054	4502-4510	VBD	denotes	revealed
T3055	4511-4515	IN	denotes	that
T3057	4516-4523	NN	denotes	Annexin
T3058	4524-4526	NN	denotes	A7
T3059	4527-4529	VBZ	denotes	is
T3060	4530-4536	RB	denotes	mainly
T3056	4537-4546	VBN	denotes	localized
T3061	4547-4549	IN	denotes	in
T3062	4550-4553	DT	denotes	the
T3063	4554-4561	NN	denotes	cytosol
T3064	4562-4564	IN	denotes	at
T3065	4565-4568	NN	denotes	E13
T3066	4569-4570	-LRB-	denotes	(
T3068	4570-4574	NN	denotes	Fig.
T3069	4575-4577	CD	denotes	3B
T3070	4577-4578	,	denotes	,
T3067	4578-4579	NN	denotes	E
T3071	4579-4580	-RRB-	denotes	)
T3072	4580-4581	.	denotes	.
T3073	4581-4721	sentence	denotes	During further development the immature cells have rounded up two days later, but they retain Annexin A7 in the cytosol at E15 (Fig. 3C,F).
T3074	4582-4588	IN	denotes	During
T3076	4589-4596	JJ	denotes	further
T3077	4597-4608	NN	denotes	development
T3078	4609-4612	DT	denotes	the
T3080	4613-4621	JJ	denotes	immature
T3079	4622-4627	NNS	denotes	cells
T3081	4628-4632	VBP	denotes	have
T3075	4633-4640	VBN	denotes	rounded
T3082	4641-4643	RP	denotes	up
T3083	4644-4647	CD	denotes	two
T3084	4648-4652	NNS	denotes	days
T3085	4653-4658	RB	denotes	later
T3086	4658-4660	,	denotes	,
T3087	4660-4663	CC	denotes	but
T3088	4664-4668	PRP	denotes	they
T3089	4669-4675	VBP	denotes	retain
T3090	4676-4683	NN	denotes	Annexin
T3091	4684-4686	NN	denotes	A7
T3092	4687-4689	IN	denotes	in
T3093	4690-4693	DT	denotes	the
T3094	4694-4701	NN	denotes	cytosol
T3095	4702-4704	IN	denotes	at
T3096	4705-4708	NN	denotes	E15
T3097	4709-4710	-LRB-	denotes	(
T3099	4710-4714	NN	denotes	Fig.
T3100	4715-4717	CD	denotes	3C
T3101	4717-4718	,	denotes	,
T3098	4718-4719	NN	denotes	F
T3102	4719-4720	-RRB-	denotes	)
T3103	4720-4721	.	denotes	.
T3104	4721-5009	sentence	denotes	At E16 we observed the first prominent nuclear staining of Annexin A7 (Fig. 3D,G) in cells of the intermediate zone (Fig. 3I, oval), which contains neurons radially migrating towards the growing neopallium cortex [13,14] and also glial cells forming the white matter of the adult cortex.
T3105	4722-4724	IN	denotes	At
T3107	4725-4728	NN	denotes	E16
T3108	4729-4731	PRP	denotes	we
T3106	4732-4740	VBD	denotes	observed
T3109	4741-4744	DT	denotes	the
T3111	4745-4750	JJ	denotes	first
T3112	4751-4760	JJ	denotes	prominent
T3113	4761-4768	JJ	denotes	nuclear
T3110	4769-4777	NN	denotes	staining
T3114	4778-4780	IN	denotes	of
T3115	4781-4788	NN	denotes	Annexin
T3116	4789-4791	NN	denotes	A7
T3117	4792-4793	-LRB-	denotes	(
T3119	4793-4797	NN	denotes	Fig.
T3120	4798-4800	NN	denotes	3D
T3121	4800-4801	,	denotes	,
T3118	4801-4802	NN	denotes	G
T3122	4802-4803	-RRB-	denotes	)
T3123	4804-4806	IN	denotes	in
T3124	4807-4812	NNS	denotes	cells
T3125	4813-4815	IN	denotes	of
T3126	4816-4819	DT	denotes	the
T3128	4820-4832	JJ	denotes	intermediate
T3127	4833-4837	NN	denotes	zone
T3129	4838-4839	-LRB-	denotes	(
T3131	4839-4843	NN	denotes	Fig.
T3132	4844-4846	NN	denotes	3I
T3133	4846-4848	,	denotes	,
T3130	4848-4852	NN	denotes	oval
T3134	4852-4853	-RRB-	denotes	)
T3135	4853-4855	,	denotes	,
T3136	4855-4860	WDT	denotes	which
T3137	4861-4869	VBZ	denotes	contains
T3138	4870-4877	NNS	denotes	neurons
T3139	4878-4886	RB	denotes	radially
T3140	4887-4896	VBG	denotes	migrating
T3141	4897-4904	IN	denotes	towards
T3142	4905-4908	DT	denotes	the
T3144	4909-4916	VBG	denotes	growing
T3145	4917-4927	NN	denotes	neopallium
T3143	4928-4934	NN	denotes	cortex
T3146	4935-4936	-LRB-	denotes	[
T3148	4936-4938	CD	denotes	13
T3149	4938-4939	,	denotes	,
T3147	4939-4941	CD	denotes	14
T3150	4941-4942	-RRB-	denotes	]
T3151	4943-4946	CC	denotes	and
T3152	4947-4951	RB	denotes	also
T3153	4952-4957	JJ	denotes	glial
T3154	4958-4963	NNS	denotes	cells
T3155	4964-4971	VBG	denotes	forming
T3156	4972-4975	DT	denotes	the
T3158	4976-4981	JJ	denotes	white
T3157	4982-4988	NN	denotes	matter
T3159	4989-4991	IN	denotes	of
T3160	4992-4995	DT	denotes	the
T3162	4996-5001	JJ	denotes	adult
T3161	5002-5008	NN	denotes	cortex
T3163	5008-5009	.	denotes	.
T3164	5009-5089	sentence	denotes	Cells located marginally in the neopallial cortex also exhibit a nuclear stain.
T3165	5010-5015	NNS	denotes	Cells
T3167	5016-5023	VBN	denotes	located
T3168	5024-5034	RB	denotes	marginally
T3169	5035-5037	IN	denotes	in
T3170	5038-5041	DT	denotes	the
T3172	5042-5052	JJ	denotes	neopallial
T3171	5053-5059	NN	denotes	cortex
T3173	5060-5064	RB	denotes	also
T3166	5065-5072	VBP	denotes	exhibit
T3174	5073-5074	DT	denotes	a
T3176	5075-5082	JJ	denotes	nuclear
T3175	5083-5088	NN	denotes	stain
T3177	5088-5089	.	denotes	.
T3178	5089-5222	sentence	denotes	Labelling in the ventricular germinative zone is less prominent and the cytosol is no longer more strongly stained than the nucleus.
T3179	5090-5099	NN	denotes	Labelling
T3181	5100-5102	IN	denotes	in
T3182	5103-5106	DT	denotes	the
T3184	5107-5118	JJ	denotes	ventricular
T3185	5119-5130	JJ	denotes	germinative
T3183	5131-5135	NN	denotes	zone
T3180	5136-5138	VBZ	denotes	is
T3186	5139-5143	RBR	denotes	less
T3187	5144-5153	JJ	denotes	prominent
T3188	5154-5157	CC	denotes	and
T3189	5158-5161	DT	denotes	the
T3190	5162-5169	NN	denotes	cytosol
T3191	5170-5172	VBZ	denotes	is
T3192	5173-5175	RB	denotes	no
T3193	5176-5182	RB	denotes	longer
T3194	5183-5187	RBR	denotes	more
T3195	5188-5196	RB	denotes	strongly
T3196	5197-5204	VBN	denotes	stained
T3197	5205-5209	IN	denotes	than
T3198	5210-5213	DT	denotes	the
T3199	5214-5221	NN	denotes	nucleus
T3200	5221-5222	.	denotes	.
T12790	5233-5244	JJ	denotes	Subcellular
T12791	5245-5257	NN	denotes	localization
T12792	5258-5260	IN	denotes	of
T12793	5261-5268	NN	denotes	Annexin
T12794	5269-5271	NN	denotes	A7
T12795	5272-5274	IN	denotes	in
T12796	5275-5282	NNS	denotes	embryos
T12797	5283-5286	NN	denotes	E13
T12798	5286-5288	,	denotes	,
T12799	5288-5291	NN	denotes	E15
T12800	5292-5295	CC	denotes	and
T12801	5296-5299	NN	denotes	E16
T12802	5299-5300	.	denotes	.
T12803	5300-5377	sentence	denotes	Paraffin sections of embryonic brain were stained with purified mAb 203–217.
T12804	5301-5309	NN	denotes	Paraffin
T12805	5310-5318	NNS	denotes	sections
T12807	5319-5321	IN	denotes	of
T12808	5322-5331	JJ	denotes	embryonic
T12809	5332-5337	NN	denotes	brain
T12810	5338-5342	VBD	denotes	were
T12806	5343-5350	VBN	denotes	stained
T12811	5351-5355	IN	denotes	with
T12812	5356-5364	VBN	denotes	purified
T12814	5365-5368	NN	denotes	mAb
T12813	5369-5372	CD	denotes	203
T12815	5372-5373	SYM	denotes	–
T12816	5373-5376	CD	denotes	217
T12817	5376-5377	.	denotes	.
T12818	5377-5455	sentence	denotes	Annexin A7 was visualized with Alexa Fluor 488-conjugated secondary antibody.
T12819	5378-5385	NN	denotes	Annexin
T12820	5386-5388	NN	denotes	A7
T12822	5389-5392	VBD	denotes	was
T12821	5393-5403	VBN	denotes	visualized
T12823	5404-5408	IN	denotes	with
T12824	5409-5414	NNP	denotes	Alexa
T12825	5415-5420	NNP	denotes	Fluor
T12827	5421-5424	CD	denotes	488
T12828	5424-5425	HYPH	denotes	-
T12826	5425-5435	VBN	denotes	conjugated
T12830	5436-5445	JJ	denotes	secondary
T12829	5446-5454	NN	denotes	antibody
T12831	5454-5455	.	denotes	.
T12832	5455-5668	sentence	denotes	(A) Overview, at E16 the immature GFAP-negative cells of the forming cerebral neocortex (b) surrounding the lateral ventricle (a) are strongly stained; square, a higher magnification of this area is given in (I).
T12833	5456-5457	-LRB-	denotes	(
T12834	5457-5458	LS	denotes	A
T12836	5458-5459	-RRB-	denotes	)
T12837	5460-5468	NN	denotes	Overview
T12839	5468-5470	,	denotes	,
T12840	5470-5472	IN	denotes	at
T12841	5473-5476	NN	denotes	E16
T12842	5477-5480	DT	denotes	the
T12844	5481-5489	JJ	denotes	immature
T12845	5490-5494	NN	denotes	GFAP
T12847	5494-5495	HYPH	denotes	-
T12846	5495-5503	JJ	denotes	negative
T12843	5504-5509	NNS	denotes	cells
T12848	5510-5512	IN	denotes	of
T12849	5513-5516	DT	denotes	the
T12851	5517-5524	VBG	denotes	forming
T12852	5525-5533	JJ	denotes	cerebral
T12850	5534-5543	NN	denotes	neocortex
T12853	5544-5545	-LRB-	denotes	(
T12854	5545-5546	NN	denotes	b
T12855	5546-5547	-RRB-	denotes	)
T12856	5548-5559	VBG	denotes	surrounding
T12857	5560-5563	DT	denotes	the
T12859	5564-5571	JJ	denotes	lateral
T12858	5572-5581	NN	denotes	ventricle
T12860	5582-5583	-LRB-	denotes	(
T12861	5583-5584	NN	denotes	a
T12862	5584-5585	-RRB-	denotes	)
T12863	5586-5589	VBP	denotes	are
T12864	5590-5598	RB	denotes	strongly
T12838	5599-5606	VBN	denotes	stained
T12865	5606-5607	:	denotes	;
T12866	5608-5614	NN	denotes	square
T12867	5614-5616	,	denotes	,
T12868	5616-5617	DT	denotes	a
T12870	5618-5624	JJR	denotes	higher
T12869	5625-5638	NN	denotes	magnification
T12871	5639-5641	IN	denotes	of
T12872	5642-5646	DT	denotes	this
T12873	5647-5651	NN	denotes	area
T12874	5652-5654	VBZ	denotes	is
T12835	5655-5660	VBN	denotes	given
T12875	5661-5663	IN	denotes	in
T12876	5664-5665	-LRB-	denotes	(
T12877	5665-5666	NN	denotes	I
T12878	5666-5667	-RRB-	denotes	)
T12879	5667-5668	.	denotes	.
T12880	5668-5784	sentence	denotes	(B) Higher magnification of the earlier stage E13 (H) shows, that the cells are stained in the cytosol (arrowhead).
T12881	5669-5670	-LRB-	denotes	(
T12882	5670-5671	LS	denotes	B
T12884	5671-5672	-RRB-	denotes	)
T12885	5673-5679	JJR	denotes	Higher
T12886	5680-5693	NN	denotes	magnification
T12887	5694-5696	IN	denotes	of
T12888	5697-5700	DT	denotes	the
T12890	5701-5708	JJR	denotes	earlier
T12891	5709-5714	NN	denotes	stage
T12889	5715-5718	NN	denotes	E13
T12892	5719-5720	-LRB-	denotes	(
T12893	5720-5721	NN	denotes	H
T12894	5721-5722	-RRB-	denotes	)
T12883	5723-5728	VBZ	denotes	shows
T12895	5728-5730	,	denotes	,
T12896	5730-5734	IN	denotes	that
T12898	5735-5738	DT	denotes	the
T12899	5739-5744	NNS	denotes	cells
T12900	5745-5748	VBP	denotes	are
T12897	5749-5756	VBN	denotes	stained
T12901	5757-5759	IN	denotes	in
T12902	5760-5763	DT	denotes	the
T12903	5764-5771	NN	denotes	cytosol
T12904	5772-5773	-LRB-	denotes	(
T12905	5773-5782	NN	denotes	arrowhead
T12906	5782-5783	-RRB-	denotes	)
T12907	5783-5784	.	denotes	.
T12908	5784-5874	sentence	denotes	(C) Two days later at E15 the cells have rounded up, and Annexin A7 stays in the cytosol.
T12909	5785-5786	-LRB-	denotes	(
T12910	5786-5787	LS	denotes	C
T12912	5787-5788	-RRB-	denotes	)
T12913	5789-5792	CD	denotes	Two
T12914	5793-5797	NNS	denotes	days
T12915	5798-5803	RBR	denotes	later
T12916	5804-5806	IN	denotes	at
T12917	5807-5810	NN	denotes	E15
T12918	5811-5814	DT	denotes	the
T12919	5815-5820	NNS	denotes	cells
T12920	5821-5825	VBP	denotes	have
T12911	5826-5833	VBN	denotes	rounded
T12921	5834-5836	RP	denotes	up
T12922	5836-5838	,	denotes	,
T12923	5838-5841	CC	denotes	and
T12924	5842-5849	NN	denotes	Annexin
T12925	5850-5852	NN	denotes	A7
T12926	5853-5858	VBZ	denotes	stays
T12927	5859-5861	IN	denotes	in
T12928	5862-5865	DT	denotes	the
T12929	5866-5873	NN	denotes	cytosol
T12930	5873-5874	.	denotes	.
T12931	5874-6073	sentence	denotes	(D) At E16 the first nuclear staining becomes apparent (arrowhead) in cells of the intermediate zone located between ventricular germinative zone and marginal neopallial cortex as seen in (I), oval.
T12932	5875-5876	-LRB-	denotes	(
T12933	5876-5877	LS	denotes	D
T12935	5877-5878	-RRB-	denotes	)
T12936	5879-5881	IN	denotes	At
T12937	5882-5885	NN	denotes	E16
T12938	5886-5889	DT	denotes	the
T12940	5890-5895	JJ	denotes	first
T12941	5896-5903	JJ	denotes	nuclear
T12939	5904-5912	NN	denotes	staining
T12934	5913-5920	VBZ	denotes	becomes
T12942	5921-5929	JJ	denotes	apparent
T12943	5930-5931	-LRB-	denotes	(
T12944	5931-5940	NN	denotes	arrowhead
T12945	5940-5941	-RRB-	denotes	)
T12946	5942-5944	IN	denotes	in
T12947	5945-5950	NNS	denotes	cells
T12948	5951-5953	IN	denotes	of
T12949	5954-5957	DT	denotes	the
T12951	5958-5970	JJ	denotes	intermediate
T12950	5971-5975	NN	denotes	zone
T12952	5976-5983	VBN	denotes	located
T12953	5984-5991	IN	denotes	between
T12954	5992-6003	JJ	denotes	ventricular
T12956	6004-6015	JJ	denotes	germinative
T12955	6016-6020	NN	denotes	zone
T12957	6021-6024	CC	denotes	and
T12958	6025-6033	JJ	denotes	marginal
T12960	6034-6044	JJ	denotes	neopallial
T12959	6045-6051	NN	denotes	cortex
T12961	6052-6054	IN	denotes	as
T12962	6055-6059	VBN	denotes	seen
T12963	6060-6062	IN	denotes	in
T12964	6063-6064	-LRB-	denotes	(
T12965	6064-6065	NN	denotes	I
T12966	6065-6066	-RRB-	denotes	)
T12967	6066-6068	,	denotes	,
T12968	6068-6072	NN	denotes	oval
T12969	6072-6073	.	denotes	.
T12970	6073-6128	sentence	denotes	(E-G) Confocal microscopy confirms the results in B-D.
T12971	6074-6075	-LRB-	denotes	(
T12972	6075-6076	LS	denotes	E
T12974	6076-6077	SYM	denotes	-
T12975	6077-6078	LS	denotes	G
T12976	6078-6079	-RRB-	denotes	)
T12977	6080-6088	JJ	denotes	Confocal
T12978	6089-6099	NN	denotes	microscopy
T12973	6100-6108	VBZ	denotes	confirms
T12979	6109-6112	DT	denotes	the
T12980	6113-6120	NNS	denotes	results
T12981	6121-6123	IN	denotes	in
T12982	6124-6125	NN	denotes	B
T12983	6125-6126	SYM	denotes	-
T12984	6126-6127	NN	denotes	D
T12985	6127-6128	.	denotes	.
T12986	6128-6226	sentence	denotes	(H) Overview, at E13 the immature GFAP-negative cells are strongly stained; a, lateral ventricle.
T12987	6129-6130	-LRB-	denotes	(
T12988	6130-6131	LS	denotes	H
T12990	6131-6132	-RRB-	denotes	)
T12991	6133-6141	NN	denotes	Overview
T12992	6141-6143	,	denotes	,
T12993	6143-6145	IN	denotes	at
T12994	6146-6149	NN	denotes	E13
T12995	6150-6153	DT	denotes	the
T12997	6154-6162	JJ	denotes	immature
T12998	6163-6167	NN	denotes	GFAP
T13000	6167-6168	HYPH	denotes	-
T12999	6168-6176	JJ	denotes	negative
T12996	6177-6182	NNS	denotes	cells
T13001	6183-6186	VBP	denotes	are
T13002	6187-6195	RB	denotes	strongly
T12989	6196-6203	VBN	denotes	stained
T13003	6203-6204	:	denotes	;
T13005	6205-6206	NN	denotes	a
T13006	6206-6208	,	denotes	,
T13007	6208-6215	JJ	denotes	lateral
T13004	6216-6225	NN	denotes	ventricle
T13008	6225-6226	.	denotes	.
T13009	6226-6410	sentence	denotes	(I) Higher magnification of a cortical section of (A, square) demonstrating ventricular, intermediate, and marginal zones; oval, a higher magnification of this area is given in (D,G).
T13010	6227-6228	-LRB-	denotes	(
T13011	6228-6229	LS	denotes	I
T13013	6229-6230	-RRB-	denotes	)
T13014	6231-6237	JJR	denotes	Higher
T13012	6238-6251	NN	denotes	magnification
T13015	6252-6254	IN	denotes	of
T13016	6255-6256	DT	denotes	a
T13018	6257-6265	JJ	denotes	cortical
T13017	6266-6273	NN	denotes	section
T13019	6274-6276	IN	denotes	of
T13020	6277-6278	-LRB-	denotes	(
T13021	6278-6279	NN	denotes	A
T13022	6279-6281	,	denotes	,
T13023	6281-6287	NN	denotes	square
T13024	6287-6288	-RRB-	denotes	)
T13025	6289-6302	VBG	denotes	demonstrating
T13026	6303-6314	JJ	denotes	ventricular
T13028	6314-6316	,	denotes	,
T13029	6316-6328	JJ	denotes	intermediate
T13030	6328-6330	,	denotes	,
T13031	6330-6333	CC	denotes	and
T13032	6334-6342	JJ	denotes	marginal
T13027	6343-6348	NNS	denotes	zones
T13033	6348-6349	:	denotes	;
T13034	6350-6354	NN	denotes	oval
T13036	6354-6356	,	denotes	,
T13037	6356-6357	DT	denotes	a
T13039	6358-6364	JJR	denotes	higher
T13038	6365-6378	NN	denotes	magnification
T13040	6379-6381	IN	denotes	of
T13041	6382-6386	DT	denotes	this
T13042	6387-6391	NN	denotes	area
T13043	6392-6394	VBZ	denotes	is
T13035	6395-6400	VBN	denotes	given
T13044	6401-6403	IN	denotes	in
T13045	6404-6405	-LRB-	denotes	(
T13046	6405-6406	NN	denotes	D
T13048	6406-6407	,	denotes	,
T13047	6407-6408	NN	denotes	G
T13049	6408-6409	-RRB-	denotes	)
T13050	6409-6410	.	denotes	.
T3569	6412-6418	JJ	denotes	Mature
T3570	6419-6426	NNS	denotes	neurons
T3572	6427-6429	IN	denotes	in
T3573	6430-6435	JJ	denotes	adult
T3575	6436-6441	NN	denotes	mouse
T3574	6442-6447	NN	denotes	brain
T3571	6448-6452	VBP	denotes	show
T3576	6453-6455	DT	denotes	an
T3578	6456-6463	JJ	denotes	intense
T3579	6464-6471	JJ	denotes	nuclear
T3577	6472-6480	NN	denotes	staining
T3580	6481-6483	IN	denotes	of
T3581	6484-6491	NN	denotes	Annexin
T3582	6492-6494	NN	denotes	A7
T3583	6494-6562	sentence	denotes	Both Annexin A7 isoforms are found in adult brain tissue (Fig. 1B).
T3584	6495-6499	CC	denotes	Both
T3586	6500-6507	NN	denotes	Annexin
T3587	6508-6510	NN	denotes	A7
T3585	6511-6519	NNS	denotes	isoforms
T3589	6520-6523	VBP	denotes	are
T3588	6524-6529	VBN	denotes	found
T3590	6530-6532	IN	denotes	in
T3591	6533-6538	JJ	denotes	adult
T3593	6539-6544	NN	denotes	brain
T3592	6545-6551	NN	denotes	tissue
T3594	6552-6553	-LRB-	denotes	(
T3596	6553-6557	NN	denotes	Fig.
T3595	6558-6560	NN	denotes	1B
T3597	6560-6561	-RRB-	denotes	)
T3598	6561-6562	.	denotes	.
T3599	6562-6855	sentence	denotes	In general, we observed two characteristic staining patterns for Annexin A7, a prominent nuclear stain in neurons (determined by lack of GFAP-immunoreactivity, localization, morphology) and a cytoplasmic and nuclear stain in astrocytes (GFAP-positive) in all areas of the mature murine brain.
T3600	6563-6565	IN	denotes	In
T3602	6566-6573	JJ	denotes	general
T3603	6573-6575	,	denotes	,
T3604	6575-6577	PRP	denotes	we
T3601	6578-6586	VBD	denotes	observed
T3605	6587-6590	CD	denotes	two
T3607	6591-6605	JJ	denotes	characteristic
T3608	6606-6614	NN	denotes	staining
T3606	6615-6623	NNS	denotes	patterns
T3609	6624-6627	IN	denotes	for
T3610	6628-6635	NN	denotes	Annexin
T3611	6636-6638	NN	denotes	A7
T3612	6638-6640	,	denotes	,
T3613	6640-6641	DT	denotes	a
T3615	6642-6651	JJ	denotes	prominent
T3616	6652-6659	JJ	denotes	nuclear
T3614	6660-6665	NN	denotes	stain
T3617	6666-6668	IN	denotes	in
T3618	6669-6676	NNS	denotes	neurons
T3619	6677-6678	-LRB-	denotes	(
T3620	6678-6688	VBN	denotes	determined
T3621	6689-6691	IN	denotes	by
T3622	6692-6696	NN	denotes	lack
T3623	6697-6699	IN	denotes	of
T3624	6700-6704	NN	denotes	GFAP
T3626	6704-6705	HYPH	denotes	-
T3627	6705-6721	NN	denotes	immunoreactivity
T3628	6721-6723	,	denotes	,
T3629	6723-6735	NN	denotes	localization
T3630	6735-6737	,	denotes	,
T3625	6737-6747	NN	denotes	morphology
T3631	6747-6748	-RRB-	denotes	)
T3632	6749-6752	CC	denotes	and
T3633	6753-6754	DT	denotes	a
T3635	6755-6766	JJ	denotes	cytoplasmic
T3636	6767-6770	CC	denotes	and
T3637	6771-6778	JJ	denotes	nuclear
T3634	6779-6784	NN	denotes	stain
T3638	6785-6787	IN	denotes	in
T3639	6788-6798	NNS	denotes	astrocytes
T3640	6799-6800	-LRB-	denotes	(
T3642	6800-6804	NN	denotes	GFAP
T3643	6804-6805	HYPH	denotes	-
T3641	6805-6813	JJ	denotes	positive
T3644	6813-6814	-RRB-	denotes	)
T3645	6815-6817	IN	denotes	in
T3646	6818-6821	DT	denotes	all
T3647	6822-6827	NNS	denotes	areas
T3648	6828-6830	IN	denotes	of
T3649	6831-6834	DT	denotes	the
T3651	6835-6841	JJ	denotes	mature
T3652	6842-6848	JJ	denotes	murine
T3650	6849-6854	NN	denotes	brain
T3653	6854-6855	.	denotes	.
T3654	6855-7017	sentence	denotes	In the neocortex (isocortex) Annexin A7 was strongly enriched in nuclei of neurons of all six cortical laminae (Fig. 4A, section derived from cortex temporalis).
T3655	6856-6858	IN	denotes	In
T3657	6859-6862	DT	denotes	the
T3658	6863-6872	NN	denotes	neocortex
T3659	6873-6874	-LRB-	denotes	(
T3660	6874-6883	NN	denotes	isocortex
T3661	6883-6884	-RRB-	denotes	)
T3662	6885-6892	NN	denotes	Annexin
T3663	6893-6895	NN	denotes	A7
T3664	6896-6899	VBD	denotes	was
T3665	6900-6908	RB	denotes	strongly
T3656	6909-6917	VBN	denotes	enriched
T3666	6918-6920	IN	denotes	in
T3667	6921-6927	NNS	denotes	nuclei
T3668	6928-6930	IN	denotes	of
T3669	6931-6938	NNS	denotes	neurons
T3670	6939-6941	IN	denotes	of
T3671	6942-6945	DT	denotes	all
T3673	6946-6949	CD	denotes	six
T3674	6950-6958	JJ	denotes	cortical
T3672	6959-6966	NNS	denotes	laminae
T3675	6967-6968	-LRB-	denotes	(
T3677	6968-6972	NN	denotes	Fig.
T3678	6973-6975	NN	denotes	4A
T3679	6975-6977	,	denotes	,
T3676	6977-6984	NN	denotes	section
T3680	6985-6992	VBN	denotes	derived
T3681	6993-6997	IN	denotes	from
T3682	6998-7004	NN	denotes	cortex
T3683	7005-7015	NN	denotes	temporalis
T3684	7015-7016	-RRB-	denotes	)
T3685	7016-7017	.	denotes	.
T3686	7017-7124	sentence	denotes	For control, in the adult brain type-1 astrocytes can be characterized by a positive GFAP-stain (Fig. 4B).
T3687	7018-7021	IN	denotes	For
T3689	7022-7029	NN	denotes	control
T3690	7029-7031	,	denotes	,
T3691	7031-7033	IN	denotes	in
T3692	7034-7037	DT	denotes	the
T3694	7038-7043	JJ	denotes	adult
T3693	7044-7049	NN	denotes	brain
T3695	7050-7054	NN	denotes	type
T3697	7054-7055	HYPH	denotes	-
T3698	7055-7056	CD	denotes	1
T3696	7057-7067	NNS	denotes	astrocytes
T3699	7068-7071	MD	denotes	can
T3700	7072-7074	VB	denotes	be
T3688	7075-7088	VBN	denotes	characterized
T3701	7089-7091	IN	denotes	by
T3702	7092-7093	DT	denotes	a
T3704	7094-7102	JJ	denotes	positive
T3705	7103-7107	NN	denotes	GFAP
T3706	7107-7108	HYPH	denotes	-
T3703	7108-7113	NN	denotes	stain
T3707	7114-7115	-LRB-	denotes	(
T3709	7115-7119	NN	denotes	Fig.
T3708	7120-7122	NN	denotes	4B
T3710	7122-7123	-RRB-	denotes	)
T3711	7123-7124	.	denotes	.
T3712	7124-7406	sentence	denotes	Fig. 4F demonstrates the presence of nuclear Annexin A7 in a neocortical neuron of the external granular layer (layer II) and in an astrocytic cell of the neocortical molecular layer (layer I) which additionally exhibits an intense signal in the cytoplasm and in cellular branches.
T3713	7125-7129	NN	denotes	Fig.
T3714	7130-7132	NN	denotes	4F
T3715	7133-7145	VBZ	denotes	demonstrates
T3716	7146-7149	DT	denotes	the
T3717	7150-7158	NN	denotes	presence
T3718	7159-7161	IN	denotes	of
T3719	7162-7169	JJ	denotes	nuclear
T3721	7170-7177	NN	denotes	Annexin
T3720	7178-7180	NN	denotes	A7
T3722	7181-7183	IN	denotes	in
T3723	7184-7185	DT	denotes	a
T3725	7186-7197	JJ	denotes	neocortical
T3724	7198-7204	NN	denotes	neuron
T3726	7205-7207	IN	denotes	of
T3727	7208-7211	DT	denotes	the
T3729	7212-7220	JJ	denotes	external
T3730	7221-7229	JJ	denotes	granular
T3728	7230-7235	NN	denotes	layer
T3731	7236-7237	-LRB-	denotes	(
T3732	7237-7242	NN	denotes	layer
T3733	7243-7245	CD	denotes	II
T3734	7245-7246	-RRB-	denotes	)
T3735	7247-7250	CC	denotes	and
T3736	7251-7253	IN	denotes	in
T3737	7254-7256	DT	denotes	an
T3739	7257-7267	JJ	denotes	astrocytic
T3738	7268-7272	NN	denotes	cell
T3740	7273-7275	IN	denotes	of
T3741	7276-7279	DT	denotes	the
T3743	7280-7291	JJ	denotes	neocortical
T3744	7292-7301	JJ	denotes	molecular
T3742	7302-7307	NN	denotes	layer
T3745	7308-7309	-LRB-	denotes	(
T3746	7309-7314	NN	denotes	layer
T3747	7315-7316	CD	denotes	I
T3748	7316-7317	-RRB-	denotes	)
T3749	7318-7323	WDT	denotes	which
T3751	7324-7336	RB	denotes	additionally
T3750	7337-7345	VBZ	denotes	exhibits
T3752	7346-7348	DT	denotes	an
T3754	7349-7356	JJ	denotes	intense
T3753	7357-7363	NN	denotes	signal
T3755	7364-7366	IN	denotes	in
T3756	7367-7370	DT	denotes	the
T3757	7371-7380	NN	denotes	cytoplasm
T3758	7381-7384	CC	denotes	and
T3759	7385-7387	IN	denotes	in
T3760	7388-7396	JJ	denotes	cellular
T3761	7397-7405	NNS	denotes	branches
T3762	7405-7406	.	denotes	.
T3763	7406-7603	sentence	denotes	A further cell type of the isocortex showed a strong immunoreactivity in both, nucleus and cytosol, and was identified as pyramidal neuron based on morphology and absence of GFAP immunoreactivity.
T3764	7407-7408	DT	denotes	A
T3766	7409-7416	JJ	denotes	further
T3767	7417-7421	NN	denotes	cell
T3765	7422-7426	NN	denotes	type
T3769	7427-7429	IN	denotes	of
T3770	7430-7433	DT	denotes	the
T3771	7434-7443	NN	denotes	isocortex
T3768	7444-7450	VBD	denotes	showed
T3772	7451-7452	DT	denotes	a
T3774	7453-7459	JJ	denotes	strong
T3773	7460-7476	NN	denotes	immunoreactivity
T3775	7477-7479	IN	denotes	in
T3776	7480-7484	CC	denotes	both
T3778	7484-7486	,	denotes	,
T3777	7486-7493	NN	denotes	nucleus
T3779	7494-7497	CC	denotes	and
T3780	7498-7505	NN	denotes	cytosol
T3781	7505-7507	,	denotes	,
T3782	7507-7510	CC	denotes	and
T3783	7511-7514	VBD	denotes	was
T3784	7515-7525	VBN	denotes	identified
T3785	7526-7528	IN	denotes	as
T3786	7529-7538	JJ	denotes	pyramidal
T3787	7539-7545	NN	denotes	neuron
T3788	7546-7551	VBN	denotes	based
T3789	7552-7554	IN	denotes	on
T3790	7555-7565	NN	denotes	morphology
T3791	7566-7569	CC	denotes	and
T3792	7570-7577	NN	denotes	absence
T3793	7578-7580	IN	denotes	of
T3794	7581-7585	NN	denotes	GFAP
T3795	7586-7602	NN	denotes	immunoreactivity
T3796	7602-7603	.	denotes	.
T3797	7603-7677	sentence	denotes	However, Annexin A7 apparently is more enriched in the nucleus (Fig. 4E).
T3798	7604-7611	RB	denotes	However
T3800	7611-7613	,	denotes	,
T3801	7613-7620	NN	denotes	Annexin
T3802	7621-7623	NN	denotes	A7
T3803	7624-7634	RB	denotes	apparently
T3799	7635-7637	VBZ	denotes	is
T3804	7638-7642	RBR	denotes	more
T3805	7643-7651	JJ	denotes	enriched
T3806	7652-7654	IN	denotes	in
T3807	7655-7658	DT	denotes	the
T3808	7659-7666	NN	denotes	nucleus
T3809	7667-7668	-LRB-	denotes	(
T3811	7668-7672	NN	denotes	Fig.
T3810	7673-7675	NN	denotes	4E
T3812	7675-7676	-RRB-	denotes	)
T3813	7676-7677	.	denotes	.
T3814	7677-7678	sentence	denotes
T13536	7688-7695	NN	denotes	Annexin
T13537	7696-7698	NN	denotes	A7
T13538	7699-7701	VBZ	denotes	is
T13539	7702-7709	JJ	denotes	present
T13540	7710-7712	IN	denotes	in
T13541	7713-7720	NNS	denotes	neurons
T13542	7721-7724	CC	denotes	and
T13543	7725-7735	NNS	denotes	astrocytes
T13544	7736-7738	IN	denotes	of
T13545	7739-7742	DT	denotes	the
T13547	7743-7749	NN	denotes	cortex
T13546	7750-7760	NN	denotes	temporalis
T13548	7761-7764	CC	denotes	and
T13549	7765-7776	JJ	denotes	hippocampal
T13550	7777-7786	NN	denotes	formation
T13551	7787-7789	IN	denotes	of
T13552	7790-7792	CD	denotes	10
T13554	7792-7793	HYPH	denotes	-
T13553	7793-7798	NNS	denotes	weeks
T13556	7798-7799	HYPH	denotes	-
T13555	7799-7802	JJ	denotes	old
T13557	7803-7807	NNS	denotes	mice
T13558	7807-7808	.	denotes	.
T13559	7808-8009	sentence	denotes	(A) Low magnification of the cortex temporalis presents an Annexin A7 expression in cells of the pial border, in neurons of all six isocortical laminae, and a weak signal in the adjacent white matter.
T13560	7809-7810	-LRB-	denotes	(
T13561	7810-7811	LS	denotes	A
T13563	7811-7812	-RRB-	denotes	)
T13564	7813-7816	JJ	denotes	Low
T13565	7817-7830	NN	denotes	magnification
T13566	7831-7833	IN	denotes	of
T13567	7834-7837	DT	denotes	the
T13569	7838-7844	NN	denotes	cortex
T13568	7845-7855	NN	denotes	temporalis
T13562	7856-7864	VBZ	denotes	presents
T13570	7865-7867	DT	denotes	an
T13572	7868-7875	NN	denotes	Annexin
T13573	7876-7878	NN	denotes	A7
T13571	7879-7889	NN	denotes	expression
T13574	7890-7892	IN	denotes	in
T13575	7893-7898	NNS	denotes	cells
T13576	7899-7901	IN	denotes	of
T13577	7902-7905	DT	denotes	the
T13579	7906-7910	JJ	denotes	pial
T13578	7911-7917	NN	denotes	border
T13580	7917-7919	,	denotes	,
T13581	7919-7921	IN	denotes	in
T13582	7922-7929	NNS	denotes	neurons
T13583	7930-7932	IN	denotes	of
T13584	7933-7936	DT	denotes	all
T13586	7937-7940	CD	denotes	six
T13587	7941-7952	JJ	denotes	isocortical
T13585	7953-7960	NNS	denotes	laminae
T13588	7960-7962	,	denotes	,
T13589	7962-7965	CC	denotes	and
T13590	7966-7967	DT	denotes	a
T13592	7968-7972	JJ	denotes	weak
T13591	7973-7979	NN	denotes	signal
T13593	7980-7982	IN	denotes	in
T13594	7983-7986	DT	denotes	the
T13596	7987-7995	JJ	denotes	adjacent
T13597	7996-8001	JJ	denotes	white
T13595	8002-8008	NN	denotes	matter
T13598	8008-8009	.	denotes	.
T13599	8009-8054	sentence	denotes	(B) Corresponding section stained with GFAP.
T13600	8010-8011	-LRB-	denotes	(
T13601	8011-8012	LS	denotes	B
T13603	8012-8013	-RRB-	denotes	)
T13604	8014-8027	JJ	denotes	Corresponding
T13602	8028-8035	NN	denotes	section
T13605	8036-8043	VBD	denotes	stained
T13606	8044-8048	IN	denotes	with
T13607	8049-8053	NN	denotes	GFAP
T13608	8053-8054	.	denotes	.
T13609	8054-8216	sentence	denotes	(C) Staining in the Stratum pyramidalis (a) and in the dentate gyrus of the hippocampus; square, a higher magnification of acorresponding area is given in (G,H).
T13610	8055-8056	-LRB-	denotes	(
T13611	8056-8057	LS	denotes	C
T13613	8057-8058	-RRB-	denotes	)
T13612	8059-8067	NN	denotes	Staining
T13614	8068-8070	IN	denotes	in
T13615	8071-8074	DT	denotes	the
T13617	8075-8082	NN	denotes	Stratum
T13616	8083-8094	NN	denotes	pyramidalis
T13618	8095-8096	-LRB-	denotes	(
T13619	8096-8097	NN	denotes	a
T13620	8097-8098	-RRB-	denotes	)
T13621	8099-8102	CC	denotes	and
T13622	8103-8105	IN	denotes	in
T13623	8106-8109	DT	denotes	the
T13625	8110-8117	NN	denotes	dentate
T13624	8118-8123	NN	denotes	gyrus
T13626	8124-8126	IN	denotes	of
T13627	8127-8130	DT	denotes	the
T13628	8131-8142	NN	denotes	hippocampus
T13629	8142-8143	:	denotes	;
T13630	8144-8150	NN	denotes	square
T13632	8150-8152	,	denotes	,
T13633	8152-8153	DT	denotes	a
T13635	8154-8160	JJR	denotes	higher
T13634	8161-8174	NN	denotes	magnification
T13636	8175-8177	IN	denotes	of
T13637	8178-8192	JJ	denotes	acorresponding
T13638	8193-8197	NN	denotes	area
T13639	8198-8200	VBZ	denotes	is
T13631	8201-8206	VBN	denotes	given
T13640	8207-8209	IN	denotes	in
T13641	8210-8211	-LRB-	denotes	(
T13642	8211-8212	NN	denotes	G
T13644	8212-8213	,	denotes	,
T13643	8213-8214	NN	denotes	H
T13645	8214-8215	-RRB-	denotes	)
T13646	8215-8216	.	denotes	.
T13647	8216-8268	sentence	denotes	An intense Annexin A7 immunostaining is detectable.
T13648	8217-8219	DT	denotes	An
T13650	8220-8227	JJ	denotes	intense
T13651	8228-8235	NN	denotes	Annexin
T13652	8236-8238	NN	denotes	A7
T13649	8239-8253	NN	denotes	immunostaining
T13653	8254-8256	VBZ	denotes	is
T13654	8257-8267	JJ	denotes	detectable
T13655	8267-8268	.	denotes	.
T13656	8268-8332	sentence	denotes	(D) Corresponding section stained with secondary antibody only.
T13657	8269-8270	-LRB-	denotes	(
T13658	8270-8271	LS	denotes	D
T13660	8271-8272	-RRB-	denotes	)
T13661	8273-8286	JJ	denotes	Corresponding
T13659	8287-8294	NN	denotes	section
T13662	8295-8302	VBN	denotes	stained
T13663	8303-8307	IN	denotes	with
T13664	8308-8317	JJ	denotes	secondary
T13665	8318-8326	NN	denotes	antibody
T13666	8327-8331	RB	denotes	only
T13667	8331-8332	.	denotes	.
T13668	8332-8438	sentence	denotes	(E) Presence of Annexin A7 in pyramidal neurons (lamina pyramidalis externa) of the isocortex temporalis.
T13669	8333-8334	-LRB-	denotes	(
T13670	8334-8335	LS	denotes	E
T13672	8335-8336	-RRB-	denotes	)
T13671	8337-8345	NN	denotes	Presence
T13673	8346-8348	IN	denotes	of
T13674	8349-8356	NN	denotes	Annexin
T13675	8357-8359	NN	denotes	A7
T13676	8360-8362	IN	denotes	in
T13677	8363-8372	JJ	denotes	pyramidal
T13678	8373-8380	NNS	denotes	neurons
T13679	8381-8382	-LRB-	denotes	(
T13681	8382-8388	NN	denotes	lamina
T13682	8389-8400	NN	denotes	pyramidalis
T13680	8401-8408	NN	denotes	externa
T13683	8408-8409	-RRB-	denotes	)
T13684	8410-8412	IN	denotes	of
T13685	8413-8416	DT	denotes	the
T13687	8417-8426	NN	denotes	isocortex
T13686	8427-8437	NN	denotes	temporalis
T13688	8437-8438	.	denotes	.
T13689	8438-8535	sentence	denotes	These neurons were identified based on their morphology, distribution and lack of GFAP staining.
T13690	8439-8444	DT	denotes	These
T13691	8445-8452	NNS	denotes	neurons
T13693	8453-8457	VBD	denotes	were
T13692	8458-8468	VBN	denotes	identified
T13694	8469-8474	VBN	denotes	based
T13695	8475-8477	IN	denotes	on
T13696	8478-8483	PRP$	denotes	their
T13697	8484-8494	NN	denotes	morphology
T13698	8494-8496	,	denotes	,
T13699	8496-8508	NN	denotes	distribution
T13700	8509-8512	CC	denotes	and
T13701	8513-8517	NN	denotes	lack
T13702	8518-8520	IN	denotes	of
T13703	8521-8525	NN	denotes	GFAP
T13704	8526-8534	NN	denotes	staining
T13705	8534-8535	.	denotes	.
T13706	8535-8623	sentence	denotes	AnnexinA7 exhibits a punctate staining, which is pronounced in the nucleus (arrowhead).
T13707	8536-8545	NN	denotes	AnnexinA7
T13708	8546-8554	VBZ	denotes	exhibits
T13709	8555-8556	DT	denotes	a
T13711	8557-8565	NN	denotes	punctate
T13710	8566-8574	NN	denotes	staining
T13712	8574-8576	,	denotes	,
T13713	8576-8581	WDT	denotes	which
T13714	8582-8584	VBZ	denotes	is
T13715	8585-8595	JJ	denotes	pronounced
T13716	8596-8598	IN	denotes	in
T13717	8599-8602	DT	denotes	the
T13718	8603-8610	NN	denotes	nucleus
T13719	8611-8612	-LRB-	denotes	(
T13720	8612-8621	NN	denotes	arrowhead
T13721	8621-8622	-RRB-	denotes	)
T13722	8622-8623	.	denotes	.
T13723	8623-8850	sentence	denotes	(F) Higher magnification of image (A) also shows Annexin A7 in nuclei of neurons (lamina granularis externa (corpuscularis), arrowhead) and in the cytoplasm and nuclei of astrocytes (lamina molecularis, arrow; GFAP-confirmed).
T13724	8624-8625	-LRB-	denotes	(
T13725	8625-8626	LS	denotes	F
T13727	8626-8627	-RRB-	denotes	)
T13728	8628-8634	JJR	denotes	Higher
T13729	8635-8648	NN	denotes	magnification
T13730	8649-8651	IN	denotes	of
T13731	8652-8657	NN	denotes	image
T13732	8658-8659	-LRB-	denotes	(
T13733	8659-8660	NN	denotes	A
T13734	8660-8661	-RRB-	denotes	)
T13735	8662-8666	RB	denotes	also
T13726	8667-8672	VBZ	denotes	shows
T13736	8673-8680	NN	denotes	Annexin
T13737	8681-8683	NN	denotes	A7
T13738	8684-8686	IN	denotes	in
T13739	8687-8693	NNS	denotes	nuclei
T13740	8694-8696	IN	denotes	of
T13741	8697-8704	NNS	denotes	neurons
T13742	8705-8706	-LRB-	denotes	(
T13744	8706-8712	NN	denotes	lamina
T13746	8713-8723	NN	denotes	granularis
T13745	8724-8731	NN	denotes	externa
T13747	8732-8733	-LRB-	denotes	(
T13748	8733-8746	NN	denotes	corpuscularis
T13749	8746-8747	-RRB-	denotes	)
T13750	8747-8749	,	denotes	,
T13743	8749-8758	NN	denotes	arrowhead
T13751	8758-8759	-RRB-	denotes	)
T13752	8760-8763	CC	denotes	and
T13753	8764-8766	IN	denotes	in
T13754	8767-8770	DT	denotes	the
T13755	8771-8780	NN	denotes	cytoplasm
T13756	8781-8784	CC	denotes	and
T13757	8785-8791	NNS	denotes	nuclei
T13758	8792-8794	IN	denotes	of
T13759	8795-8805	NNS	denotes	astrocytes
T13760	8806-8807	-LRB-	denotes	(
T13762	8807-8813	NN	denotes	lamina
T13763	8814-8825	NN	denotes	molecularis
T13764	8825-8827	,	denotes	,
T13761	8827-8832	NN	denotes	arrow
T13765	8832-8833	:	denotes	;
T13766	8834-8838	NN	denotes	GFAP
T13768	8838-8839	HYPH	denotes	-
T13767	8839-8848	VBN	denotes	confirmed
T13769	8848-8849	-RRB-	denotes	)
T13770	8849-8850	.	denotes	.
T13771	8850-9013	sentence	denotes	(G) Higher magnification of the pyramidal neurons in the hippocampus confirms the presence of the Annexin A7 protein in the nucleus (arrowhead) of mature neurons.
T13772	8851-8852	-LRB-	denotes	(
T13773	8852-8853	LS	denotes	G
T13775	8853-8854	-RRB-	denotes	)
T13776	8855-8861	JJR	denotes	Higher
T13777	8862-8875	NN	denotes	magnification
T13778	8876-8878	IN	denotes	of
T13779	8879-8882	DT	denotes	the
T13781	8883-8892	JJ	denotes	pyramidal
T13780	8893-8900	NNS	denotes	neurons
T13782	8901-8903	IN	denotes	in
T13783	8904-8907	DT	denotes	the
T13784	8908-8919	NN	denotes	hippocampus
T13774	8920-8928	VBZ	denotes	confirms
T13785	8929-8932	DT	denotes	the
T13786	8933-8941	NN	denotes	presence
T13787	8942-8944	IN	denotes	of
T13788	8945-8948	DT	denotes	the
T13790	8949-8956	NN	denotes	Annexin
T13791	8957-8959	NN	denotes	A7
T13789	8960-8967	NN	denotes	protein
T13792	8968-8970	IN	denotes	in
T13793	8971-8974	DT	denotes	the
T13794	8975-8982	NN	denotes	nucleus
T13795	8983-8984	-LRB-	denotes	(
T13796	8984-8993	NN	denotes	arrowhead
T13797	8993-8994	-RRB-	denotes	)
T13798	8995-8997	IN	denotes	of
T13799	8998-9004	JJ	denotes	mature
T13800	9005-9012	NNS	denotes	neurons
T13801	9012-9013	.	denotes	.
T13802	9013-9169	sentence	denotes	(H) To further confirm this, a similar section derived from an AnxA7-/- mouse was stained with the annexin specific antibody and lacked the nuclear signal.
T13803	9014-9015	-LRB-	denotes	(
T13804	9015-9016	LS	denotes	H
T13806	9016-9017	-RRB-	denotes	)
T13807	9018-9020	TO	denotes	To
T13809	9021-9028	RB	denotes	further
T13808	9029-9036	VB	denotes	confirm
T13810	9037-9041	DT	denotes	this
T13811	9041-9043	,	denotes	,
T13812	9043-9044	DT	denotes	a
T13814	9045-9052	JJ	denotes	similar
T13813	9053-9060	NN	denotes	section
T13815	9061-9068	VBN	denotes	derived
T13816	9069-9073	IN	denotes	from
T13817	9074-9076	DT	denotes	an
T13819	9077-9082	NN	denotes	AnxA7
T13820	9082-9083	SYM	denotes	-
T13821	9083-9084	HYPH	denotes	/
T13822	9084-9085	SYM	denotes	-
T13818	9086-9091	NN	denotes	mouse
T13823	9092-9095	VBD	denotes	was
T13805	9096-9103	VBN	denotes	stained
T13824	9104-9108	IN	denotes	with
T13825	9109-9112	DT	denotes	the
T13827	9113-9120	NN	denotes	annexin
T13828	9121-9129	JJ	denotes	specific
T13826	9130-9138	NN	denotes	antibody
T13829	9139-9142	CC	denotes	and
T13830	9143-9149	VBD	denotes	lacked
T13831	9150-9153	DT	denotes	the
T13833	9154-9161	JJ	denotes	nuclear
T13832	9162-9168	NN	denotes	signal
T13834	9168-9169	.	denotes	.
T13835	9169-9322	sentence	denotes	The residual stain of the tissue is unspecific, as it is also observed in controls of the AnxA7-/- brain omitting the primary antibody (data not shown).
T13836	9170-9173	DT	denotes	The
T13838	9174-9182	JJ	denotes	residual
T13837	9183-9188	NN	denotes	stain
T13840	9189-9191	IN	denotes	of
T13841	9192-9195	DT	denotes	the
T13842	9196-9202	NN	denotes	tissue
T13839	9203-9205	VBZ	denotes	is
T13843	9206-9216	JJ	denotes	unspecific
T13844	9216-9218	,	denotes	,
T13845	9218-9220	IN	denotes	as
T13847	9221-9223	PRP	denotes	it
T13848	9224-9226	VBZ	denotes	is
T13849	9227-9231	RB	denotes	also
T13846	9232-9240	VBN	denotes	observed
T13850	9241-9243	IN	denotes	in
T13851	9244-9252	NNS	denotes	controls
T13852	9253-9255	IN	denotes	of
T13853	9256-9259	DT	denotes	the
T13855	9260-9265	NN	denotes	AnxA7
T13856	9265-9266	SYM	denotes	-
T13857	9266-9267	HYPH	denotes	/
T13858	9267-9268	SYM	denotes	-
T13854	9269-9274	NN	denotes	brain
T13859	9275-9283	VBG	denotes	omitting
T13860	9284-9287	DT	denotes	the
T13862	9288-9295	JJ	denotes	primary
T13861	9296-9304	NN	denotes	antibody
T13863	9305-9306	-LRB-	denotes	(
T13865	9306-9310	NNS	denotes	data
T13866	9311-9314	RB	denotes	not
T13864	9315-9320	VBN	denotes	shown
T13867	9320-9321	-RRB-	denotes	)
T13868	9321-9322	.	denotes	.
T13869	9322-9417	sentence	denotes	All paraffin sections were stained with mAb 203–217 (A, C, E, F, G) or anti-GFAP-antibody (B).
T13870	9323-9326	DT	denotes	All
T13872	9327-9335	NN	denotes	paraffin
T13871	9336-9344	NNS	denotes	sections
T13874	9345-9349	VBD	denotes	were
T13873	9350-9357	VBN	denotes	stained
T13875	9358-9362	IN	denotes	with
T13876	9363-9366	NN	denotes	mAb
T13877	9367-9370	CD	denotes	203
T13878	9370-9371	SYM	denotes	–
T13879	9371-9374	CD	denotes	217
T13880	9375-9376	-LRB-	denotes	(
T13882	9376-9377	NN	denotes	A
T13883	9377-9379	,	denotes	,
T13884	9379-9380	NN	denotes	C
T13885	9380-9382	,	denotes	,
T13886	9382-9383	NN	denotes	E
T13887	9383-9385	,	denotes	,
T13888	9385-9386	NN	denotes	F
T13889	9386-9388	,	denotes	,
T13881	9388-9389	NN	denotes	G
T13890	9389-9390	-RRB-	denotes	)
T13891	9391-9393	CC	denotes	or
T13892	9394-9403	JJ	denotes	anti-GFAP
T13894	9403-9404	HYPH	denotes	-
T13893	9404-9412	NN	denotes	antibody
T13895	9413-9414	-LRB-	denotes	(
T13896	9414-9415	NN	denotes	B
T13897	9415-9416	-RRB-	denotes	)
T13898	9416-9417	.	denotes	.
T13899	9417-9481	sentence	denotes	The hippocampal control section (D) lacks the primary antibody.
T13900	9418-9421	DT	denotes	The
T13902	9422-9433	JJ	denotes	hippocampal
T13903	9434-9441	NN	denotes	control
T13901	9442-9449	NN	denotes	section
T13905	9450-9451	-LRB-	denotes	(
T13906	9451-9452	NN	denotes	D
T13907	9452-9453	-RRB-	denotes	)
T13904	9454-9459	VBZ	denotes	lacks
T13908	9460-9463	DT	denotes	the
T13910	9464-9471	JJ	denotes	primary
T13909	9472-9480	NN	denotes	antibody
T13911	9480-9481	.	denotes	.
T3816	9482-9484	IN	denotes	In
T3815	9482-9657	sentence	denotes	In the hippocampal formation we detected prominent Annexin A7 immunostaining in the stratum pyramidalis and in the dentate gyrus and also a weak astrocytic staining (Fig. 4C).
T3818	9485-9488	DT	denotes	the
T3820	9489-9500	JJ	denotes	hippocampal
T3819	9501-9510	NN	denotes	formation
T3821	9511-9513	PRP	denotes	we
T3817	9514-9522	VBD	denotes	detected
T3822	9523-9532	JJ	denotes	prominent
T3824	9533-9540	NN	denotes	Annexin
T3825	9541-9543	NN	denotes	A7
T3823	9544-9558	NN	denotes	immunostaining
T3826	9559-9561	IN	denotes	in
T3827	9562-9565	DT	denotes	the
T3829	9566-9573	NN	denotes	stratum
T3828	9574-9585	NN	denotes	pyramidalis
T3830	9586-9589	CC	denotes	and
T3831	9590-9592	IN	denotes	in
T3832	9593-9596	DT	denotes	the
T3834	9597-9604	NN	denotes	dentate
T3833	9605-9610	NN	denotes	gyrus
T3835	9611-9614	CC	denotes	and
T3836	9615-9619	RB	denotes	also
T3837	9620-9621	DT	denotes	a
T3839	9622-9626	JJ	denotes	weak
T3840	9627-9637	JJ	denotes	astrocytic
T3838	9638-9646	NN	denotes	staining
T3841	9647-9648	-LRB-	denotes	(
T3843	9648-9652	NN	denotes	Fig.
T3842	9653-9655	NN	denotes	4C
T3844	9655-9656	-RRB-	denotes	)
T3845	9656-9657	.	denotes	.
T3846	9657-9835	sentence	denotes	The astrocytes are mainly localized in the area between cells exhibiting nuclear Annexin A7 staining and show the protein also in the nucleus and the cytoplasm (data not shown).
T3847	9658-9661	DT	denotes	The
T3848	9662-9672	NNS	denotes	astrocytes
T3850	9673-9676	VBP	denotes	are
T3851	9677-9683	RB	denotes	mainly
T3849	9684-9693	VBN	denotes	localized
T3852	9694-9696	IN	denotes	in
T3853	9697-9700	DT	denotes	the
T3854	9701-9705	NN	denotes	area
T3855	9706-9713	IN	denotes	between
T3856	9714-9719	NNS	denotes	cells
T3857	9720-9730	VBG	denotes	exhibiting
T3858	9731-9738	JJ	denotes	nuclear
T3860	9739-9746	NN	denotes	Annexin
T3861	9747-9749	NN	denotes	A7
T3859	9750-9758	NN	denotes	staining
T3862	9759-9762	CC	denotes	and
T3863	9763-9767	VBP	denotes	show
T3864	9768-9771	DT	denotes	the
T3865	9772-9779	NN	denotes	protein
T3866	9780-9784	RB	denotes	also
T3867	9785-9787	IN	denotes	in
T3868	9788-9791	DT	denotes	the
T3869	9792-9799	NN	denotes	nucleus
T3870	9800-9803	CC	denotes	and
T3871	9804-9807	DT	denotes	the
T3872	9808-9817	NN	denotes	cytoplasm
T3873	9818-9819	-LRB-	denotes	(
T3875	9819-9823	NNS	denotes	data
T3876	9824-9827	RB	denotes	not
T3874	9828-9833	VBN	denotes	shown
T3877	9833-9834	-RRB-	denotes	)
T3878	9834-9835	.	denotes	.
T3879	9835-9992	sentence	denotes	When we analyzed the pyramidal neurons in the hippocampus at a higher magnification we found that Annexin A7 again mainly localized to the nuclei (Fig. 4G).
T3880	9836-9840	WRB	denotes	When
T3882	9841-9843	PRP	denotes	we
T3881	9844-9852	VBD	denotes	analyzed
T3884	9853-9856	DT	denotes	the
T3886	9857-9866	JJ	denotes	pyramidal
T3885	9867-9874	NNS	denotes	neurons
T3887	9875-9877	IN	denotes	in
T3888	9878-9881	DT	denotes	the
T3889	9882-9893	NN	denotes	hippocampus
T3890	9894-9896	IN	denotes	at
T3891	9897-9898	DT	denotes	a
T3893	9899-9905	JJR	denotes	higher
T3892	9906-9919	NN	denotes	magnification
T3894	9920-9922	PRP	denotes	we
T3883	9923-9928	VBD	denotes	found
T3895	9929-9933	IN	denotes	that
T3897	9934-9941	NN	denotes	Annexin
T3898	9942-9944	NN	denotes	A7
T3899	9945-9950	RB	denotes	again
T3900	9951-9957	RB	denotes	mainly
T3896	9958-9967	VBN	denotes	localized
T3901	9968-9970	IN	denotes	to
T3902	9971-9974	DT	denotes	the
T3903	9975-9981	NNS	denotes	nuclei
T3904	9982-9983	-LRB-	denotes	(
T3906	9983-9987	NN	denotes	Fig.
T3905	9988-9990	NN	denotes	4G
T3907	9990-9991	-RRB-	denotes	)
T3908	9991-9992	.	denotes	.
T3909	9992-10064	sentence	denotes	Polyclonal antibodies gave a similar staining pattern (data not shown).
T3910	9993-10003	JJ	denotes	Polyclonal
T3911	10004-10014	NNS	denotes	antibodies
T3912	10015-10019	VBD	denotes	gave
T3913	10020-10021	DT	denotes	a
T3915	10022-10029	JJ	denotes	similar
T3916	10030-10038	NN	denotes	staining
T3914	10039-10046	NN	denotes	pattern
T3917	10047-10048	-LRB-	denotes	(
T3919	10048-10052	NNS	denotes	data
T3920	10053-10056	RB	denotes	not
T3918	10057-10062	VBN	denotes	shown
T3921	10062-10063	-RRB-	denotes	)
T3922	10063-10064	.	denotes	.
T3923	10064-10256	sentence	denotes	The intense nuclear staining was absent in controls either stained only with the secondary antibody (Fig. 4D) or in brain sections derived from AnxA7-/- mice stained for Annexin A7 (Fig. 4H).
T3924	10065-10068	DT	denotes	The
T3926	10069-10076	JJ	denotes	intense
T3927	10077-10084	JJ	denotes	nuclear
T3925	10085-10093	NN	denotes	staining
T3928	10094-10097	VBD	denotes	was
T3929	10098-10104	JJ	denotes	absent
T3930	10105-10107	IN	denotes	in
T3931	10108-10116	NNS	denotes	controls
T3932	10117-10123	CC	denotes	either
T3933	10124-10131	VBN	denotes	stained
T3934	10132-10136	RB	denotes	only
T3935	10137-10141	IN	denotes	with
T3936	10142-10145	DT	denotes	the
T3938	10146-10155	JJ	denotes	secondary
T3937	10156-10164	NN	denotes	antibody
T3939	10165-10166	-LRB-	denotes	(
T3941	10166-10170	NN	denotes	Fig.
T3940	10171-10173	NN	denotes	4D
T3942	10173-10174	-RRB-	denotes	)
T3943	10175-10177	CC	denotes	or
T3944	10178-10180	IN	denotes	in
T3945	10181-10186	NN	denotes	brain
T3946	10187-10195	NNS	denotes	sections
T3947	10196-10203	VBN	denotes	derived
T3948	10204-10208	IN	denotes	from
T3949	10209-10214	NN	denotes	AnxA7
T3951	10214-10215	SYM	denotes	-
T3952	10215-10216	HYPH	denotes	/
T3953	10216-10217	SYM	denotes	-
T3950	10218-10222	NNS	denotes	mice
T3954	10223-10230	VBN	denotes	stained
T3955	10231-10234	IN	denotes	for
T3956	10235-10242	NN	denotes	Annexin
T3957	10243-10245	NN	denotes	A7
T3958	10246-10247	-LRB-	denotes	(
T3960	10247-10251	NN	denotes	Fig.
T3959	10252-10254	NN	denotes	4H
T3961	10254-10255	-RRB-	denotes	)
T3962	10255-10256	.	denotes	.
T3963	10256-10406	sentence	denotes	The residual staining seen in the AnxA7-/- brain is unspecific as it is also present in corresponding negative controls lacking the primary antibody.
T3964	10257-10260	DT	denotes	The
T3966	10261-10269	JJ	denotes	residual
T3965	10270-10278	NN	denotes	staining
T3968	10279-10283	VBN	denotes	seen
T3969	10284-10286	IN	denotes	in
T3970	10287-10290	DT	denotes	the
T3972	10291-10296	NN	denotes	AnxA7
T3973	10296-10297	SYM	denotes	-
T3974	10297-10298	HYPH	denotes	/
T3975	10298-10299	SYM	denotes	-
T3971	10300-10305	NN	denotes	brain
T3967	10306-10308	VBZ	denotes	is
T3976	10309-10319	JJ	denotes	unspecific
T3977	10320-10322	IN	denotes	as
T3979	10323-10325	PRP	denotes	it
T3978	10326-10328	VBZ	denotes	is
T3980	10329-10333	RB	denotes	also
T3981	10334-10341	JJ	denotes	present
T3982	10342-10344	IN	denotes	in
T3983	10345-10358	VBG	denotes	corresponding
T3985	10359-10367	JJ	denotes	negative
T3984	10368-10376	NNS	denotes	controls
T3986	10377-10384	VBG	denotes	lacking
T3987	10385-10388	DT	denotes	the
T3989	10389-10396	JJ	denotes	primary
T3988	10397-10405	NN	denotes	antibody
T3990	10405-10406	.	denotes	.
T3991	10406-10516	sentence	denotes	The absence of Annexin A7 protein in brain and other tissues of the AnxA7-/- mouse has been verified in [10].
T3992	10407-10410	DT	denotes	The
T3993	10411-10418	NN	denotes	absence
T3995	10419-10421	IN	denotes	of
T3996	10422-10429	NN	denotes	Annexin
T3997	10430-10432	NN	denotes	A7
T3998	10433-10440	NN	denotes	protein
T3999	10441-10443	IN	denotes	in
T4000	10444-10449	NN	denotes	brain
T4001	10450-10453	CC	denotes	and
T4002	10454-10459	JJ	denotes	other
T4003	10460-10467	NNS	denotes	tissues
T4004	10468-10470	IN	denotes	of
T4005	10471-10474	DT	denotes	the
T4007	10475-10480	NN	denotes	AnxA7
T4008	10480-10481	SYM	denotes	-
T4009	10481-10482	HYPH	denotes	/
T4010	10482-10483	SYM	denotes	-
T4006	10484-10489	NN	denotes	mouse
T4011	10490-10493	VBZ	denotes	has
T4012	10494-10498	VBN	denotes	been
T3994	10499-10507	VBN	denotes	verified
T4013	10508-10510	IN	denotes	in
T4014	10511-10512	-LRB-	denotes	[
T4015	10512-10514	CD	denotes	10
T4016	10514-10515	-RRB-	denotes	]
T4017	10515-10516	.	denotes	.
T4018	10516-10637	sentence	denotes	In another actual study of the knock out mouse we detected a thickened basal membrane and a widened intercellular space.
T4019	10517-10519	IN	denotes	In
T4021	10520-10527	DT	denotes	another
T4023	10528-10534	JJ	denotes	actual
T4022	10535-10540	NN	denotes	study
T4024	10541-10543	IN	denotes	of
T4025	10544-10547	DT	denotes	the
T4027	10548-10553	VB	denotes	knock
T4028	10554-10557	RP	denotes	out
T4026	10558-10563	NN	denotes	mouse
T4029	10564-10566	PRP	denotes	we
T4020	10567-10575	VBD	denotes	detected
T4030	10576-10577	DT	denotes	a
T4032	10578-10587	VBN	denotes	thickened
T4033	10588-10593	JJ	denotes	basal
T4031	10594-10602	NN	denotes	membrane
T4034	10603-10606	CC	denotes	and
T4035	10607-10608	DT	denotes	a
T4037	10609-10616	VBN	denotes	widened
T4038	10617-10630	JJ	denotes	intercellular
T4036	10631-10636	NN	denotes	space
T4039	10636-10637	.	denotes	.
T4040	10637-10698	sentence	denotes	This may cause unspecific binding of the secondary antibody.
T4041	10638-10642	DT	denotes	This
T4043	10643-10646	MD	denotes	may
T4042	10647-10652	VB	denotes	cause
T4044	10653-10663	JJ	denotes	unspecific
T4045	10664-10671	NN	denotes	binding
T4046	10672-10674	IN	denotes	of
T4047	10675-10678	DT	denotes	the
T4049	10679-10688	JJ	denotes	secondary
T4048	10689-10697	NN	denotes	antibody
T4050	10697-10698	.	denotes	.
T4051	10698-10774	sentence	denotes	An equal staining of Annexin A7 was also found in the cerebellum (Fig. 5A).
T4052	10699-10701	DT	denotes	An
T4054	10702-10707	JJ	denotes	equal
T4053	10708-10716	NN	denotes	staining
T4056	10717-10719	IN	denotes	of
T4057	10720-10727	NN	denotes	Annexin
T4058	10728-10730	NN	denotes	A7
T4059	10731-10734	VBD	denotes	was
T4060	10735-10739	RB	denotes	also
T4055	10740-10745	VBN	denotes	found
T4061	10746-10748	IN	denotes	in
T4062	10749-10752	DT	denotes	the
T4063	10753-10763	NN	denotes	cerebellum
T4064	10764-10765	-LRB-	denotes	(
T4066	10765-10769	NN	denotes	Fig.
T4065	10770-10772	NN	denotes	5A
T4067	10772-10773	-RRB-	denotes	)
T4068	10773-10774	.	denotes	.
T4069	10774-10864	sentence	denotes	Nuclei of neurons in the stratum granulosum show the most prominent staining (Fig. 5C,D).
T4070	10775-10781	NNS	denotes	Nuclei
T4072	10782-10784	IN	denotes	of
T4073	10785-10792	NNS	denotes	neurons
T4074	10793-10795	IN	denotes	in
T4075	10796-10799	DT	denotes	the
T4077	10800-10807	NN	denotes	stratum
T4076	10808-10818	NN	denotes	granulosum
T4071	10819-10823	VBP	denotes	show
T4078	10824-10827	DT	denotes	the
T4080	10828-10832	RBS	denotes	most
T4081	10833-10842	JJ	denotes	prominent
T4079	10843-10851	NN	denotes	staining
T4082	10852-10853	-LRB-	denotes	(
T4084	10853-10857	NN	denotes	Fig.
T4085	10858-10860	NN	denotes	5C
T4086	10860-10861	,	denotes	,
T4083	10861-10862	NN	denotes	D
T4087	10862-10863	-RRB-	denotes	)
T4088	10863-10864	.	denotes	.
T4089	10864-10999	sentence	denotes	In the stratum moleculare, which is poor in cell bodies of neurons, only a few dots appear which also correspond to nuclei of neurons.
T4090	10865-10867	IN	denotes	In
T4092	10868-10871	DT	denotes	the
T4094	10872-10879	NN	denotes	stratum
T4093	10880-10890	NN	denotes	moleculare
T4095	10890-10892	,	denotes	,
T4096	10892-10897	WDT	denotes	which
T4097	10898-10900	VBZ	denotes	is
T4098	10901-10905	JJ	denotes	poor
T4099	10906-10908	IN	denotes	in
T4100	10909-10913	NN	denotes	cell
T4101	10914-10920	NNS	denotes	bodies
T4102	10921-10923	IN	denotes	of
T4103	10924-10931	NNS	denotes	neurons
T4104	10931-10933	,	denotes	,
T4105	10933-10937	RB	denotes	only
T4107	10938-10939	DT	denotes	a
T4108	10940-10943	JJ	denotes	few
T4106	10944-10948	NNS	denotes	dots
T4091	10949-10955	VBP	denotes	appear
T4109	10956-10961	WDT	denotes	which
T4111	10962-10966	RB	denotes	also
T4110	10967-10977	VBP	denotes	correspond
T4112	10978-10980	IN	denotes	to
T4113	10981-10987	NNS	denotes	nuclei
T4114	10988-10990	IN	denotes	of
T4115	10991-10998	NNS	denotes	neurons
T4116	10998-10999	.	denotes	.
T4117	10999-11089	sentence	denotes	Astrocytes are also positive for Annexin A7 in the nuclei and cytoplasm (data not shown).
T4118	11000-11010	NNS	denotes	Astrocytes
T4119	11011-11014	VBP	denotes	are
T4120	11015-11019	RB	denotes	also
T4121	11020-11028	JJ	denotes	positive
T4122	11029-11032	IN	denotes	for
T4123	11033-11040	NN	denotes	Annexin
T4124	11041-11043	NN	denotes	A7
T4125	11044-11046	IN	denotes	in
T4126	11047-11050	DT	denotes	the
T4127	11051-11057	NNS	denotes	nuclei
T4128	11058-11061	CC	denotes	and
T4129	11062-11071	NN	denotes	cytoplasm
T4130	11072-11073	-LRB-	denotes	(
T4132	11073-11077	NNS	denotes	data
T4133	11078-11081	RB	denotes	not
T4131	11082-11087	VBN	denotes	shown
T4134	11087-11088	-RRB-	denotes	)
T4135	11088-11089	.	denotes	.
T4136	11089-11247	sentence	denotes	The signal of the pial border and the prominent stain of the white matter tracts (laminae medullares) are due to Annexin A7 positive astrocytes (Fig. 5 A–C).
T4137	11090-11093	DT	denotes	The
T4138	11094-11100	NN	denotes	signal
T4140	11101-11103	IN	denotes	of
T4141	11104-11107	DT	denotes	the
T4143	11108-11112	JJ	denotes	pial
T4142	11113-11119	NN	denotes	border
T4144	11120-11123	CC	denotes	and
T4145	11124-11127	DT	denotes	the
T4147	11128-11137	JJ	denotes	prominent
T4146	11138-11143	NN	denotes	stain
T4148	11144-11146	IN	denotes	of
T4149	11147-11150	DT	denotes	the
T4151	11151-11156	JJ	denotes	white
T4152	11157-11163	NN	denotes	matter
T4150	11164-11170	NNS	denotes	tracts
T4153	11171-11172	-LRB-	denotes	(
T4154	11172-11179	NNS	denotes	laminae
T4155	11180-11190	NNS	denotes	medullares
T4156	11190-11191	-RRB-	denotes	)
T4139	11192-11195	VBP	denotes	are
T4157	11196-11199	IN	denotes	due
T4158	11200-11202	IN	denotes	to
T4159	11203-11210	NN	denotes	Annexin
T4160	11211-11213	NN	denotes	A7
T4161	11214-11222	JJ	denotes	positive
T4162	11223-11233	NNS	denotes	astrocytes
T4163	11234-11235	-LRB-	denotes	(
T4165	11235-11239	NN	denotes	Fig.
T4166	11240-11241	CD	denotes	5
T4164	11242-11243	NN	denotes	A
T4167	11243-11244	SYM	denotes	–
T4168	11244-11245	NN	denotes	C
T4169	11245-11246	-RRB-	denotes	)
T4170	11246-11247	.	denotes	.
T4171	11247-11465	sentence	denotes	Higher magnification of the boundary layer between stratum moleculare and granulosum (Fig. 5D) revealed an Annexin A7 signal in nuclei, but also in the cytoplasm and at the plasma membrane of Purkinje cells (Fig. 5E).
T4172	11248-11254	JJR	denotes	Higher
T4173	11255-11268	NN	denotes	magnification
T4175	11269-11271	IN	denotes	of
T4176	11272-11275	DT	denotes	the
T4178	11276-11284	JJ	denotes	boundary
T4177	11285-11290	NN	denotes	layer
T4179	11291-11298	IN	denotes	between
T4180	11299-11306	NN	denotes	stratum
T4181	11307-11317	NN	denotes	moleculare
T4182	11318-11321	CC	denotes	and
T4183	11322-11332	NN	denotes	granulosum
T4184	11333-11334	-LRB-	denotes	(
T4186	11334-11338	NN	denotes	Fig.
T4185	11339-11341	NN	denotes	5D
T4187	11341-11342	-RRB-	denotes	)
T4174	11343-11351	VBD	denotes	revealed
T4188	11352-11354	DT	denotes	an
T4190	11355-11362	NN	denotes	Annexin
T4191	11363-11365	NN	denotes	A7
T4189	11366-11372	NN	denotes	signal
T4192	11373-11375	IN	denotes	in
T4193	11376-11382	NNS	denotes	nuclei
T4194	11382-11384	,	denotes	,
T4195	11384-11387	CC	denotes	but
T4196	11388-11392	RB	denotes	also
T4197	11393-11395	IN	denotes	in
T4198	11396-11399	DT	denotes	the
T4199	11400-11409	NN	denotes	cytoplasm
T4200	11410-11413	CC	denotes	and
T4201	11414-11416	IN	denotes	at
T4202	11417-11420	DT	denotes	the
T4204	11421-11427	NN	denotes	plasma
T4203	11428-11436	NN	denotes	membrane
T4205	11437-11439	IN	denotes	of
T4206	11440-11448	NNP	denotes	Purkinje
T4207	11449-11454	NNS	denotes	cells
T4208	11455-11456	-LRB-	denotes	(
T4210	11456-11460	NN	denotes	Fig.
T4209	11461-11463	NN	denotes	5E
T4211	11463-11464	-RRB-	denotes	)
T4212	11464-11465	.	denotes	.
T4213	11465-11542	sentence	denotes	The typical pair of dendrites points to the margin of the cerebellar cortex.
T4214	11466-11469	DT	denotes	The
T4216	11470-11477	JJ	denotes	typical
T4215	11478-11482	NN	denotes	pair
T4218	11483-11485	IN	denotes	of
T4219	11486-11495	NNS	denotes	dendrites
T4217	11496-11502	VBZ	denotes	points
T4220	11503-11505	IN	denotes	to
T4221	11506-11509	DT	denotes	the
T4222	11510-11516	NN	denotes	margin
T4223	11517-11519	IN	denotes	of
T4224	11520-11523	DT	denotes	the
T4226	11524-11534	JJ	denotes	cerebellar
T4225	11535-11541	NN	denotes	cortex
T4227	11541-11542	.	denotes	.
T4228	11542-11664	sentence	denotes	The specificity of the Annexin A7 signal was further confirmed in similar brain sections of the AnxA7-/- mouse (Fig. 5F).
T4229	11543-11546	DT	denotes	The
T4230	11547-11558	NN	denotes	specificity
T4232	11559-11561	IN	denotes	of
T4233	11562-11565	DT	denotes	the
T4235	11566-11573	NN	denotes	Annexin
T4236	11574-11576	NN	denotes	A7
T4234	11577-11583	NN	denotes	signal
T4237	11584-11587	VBD	denotes	was
T4238	11588-11595	RBR	denotes	further
T4231	11596-11605	VBN	denotes	confirmed
T4239	11606-11608	IN	denotes	in
T4240	11609-11616	JJ	denotes	similar
T4242	11617-11622	NN	denotes	brain
T4241	11623-11631	NNS	denotes	sections
T4243	11632-11634	IN	denotes	of
T4244	11635-11638	DT	denotes	the
T4246	11639-11644	NN	denotes	AnxA7
T4247	11644-11645	SYM	denotes	-
T4248	11645-11646	HYPH	denotes	/
T4249	11646-11647	SYM	denotes	-
T4245	11648-11653	NN	denotes	mouse
T4250	11654-11655	-LRB-	denotes	(
T4252	11655-11659	NN	denotes	Fig.
T4251	11660-11662	NN	denotes	5F
T4253	11662-11663	-RRB-	denotes	)
T4254	11663-11664	.	denotes	.
T4255	11664-11773	sentence	denotes	Fig. 5G shows Annexin A7 in neurites (axons) connecting the laminae medullares with the Purkinje-cell layer.
T4256	11665-11669	NN	denotes	Fig.
T4257	11670-11672	NN	denotes	5G
T4258	11673-11678	VBZ	denotes	shows
T4259	11679-11686	NN	denotes	Annexin
T4260	11687-11689	NN	denotes	A7
T4261	11690-11692	IN	denotes	in
T4262	11693-11701	NNS	denotes	neurites
T4263	11702-11703	-LRB-	denotes	(
T4264	11703-11708	NNS	denotes	axons
T4265	11708-11709	-RRB-	denotes	)
T4266	11710-11720	VBG	denotes	connecting
T4267	11721-11724	DT	denotes	the
T4269	11725-11732	NNS	denotes	laminae
T4268	11733-11743	NNS	denotes	medullares
T4270	11744-11748	IN	denotes	with
T4271	11749-11752	DT	denotes	the
T4273	11753-11761	NNP	denotes	Purkinje
T4275	11761-11762	HYPH	denotes	-
T4274	11762-11766	NN	denotes	cell
T4272	11767-11772	NN	denotes	layer
T4276	11772-11773	.	denotes	.
T4277	11773-11828	sentence	denotes	Most likely these are the axons of the Purkinje-cells.
T4278	11774-11778	RBS	denotes	Most
T4279	11779-11785	RB	denotes	likely
T4281	11786-11791	DT	denotes	these
T4280	11792-11795	VBP	denotes	are
T4282	11796-11799	DT	denotes	the
T4283	11800-11805	NNS	denotes	axons
T4284	11806-11808	IN	denotes	of
T4285	11809-11812	DT	denotes	the
T4287	11813-11821	NNP	denotes	Purkinje
T4288	11821-11822	HYPH	denotes	-
T4286	11822-11827	NNS	denotes	cells
T4289	11827-11828	.	denotes	.
T4290	11828-11958	sentence	denotes	Apart from these efferent neurites the laminae medullares (lamina alba, white matter) contain afferent mossy and climbing fibers.
T4291	11829-11834	RB	denotes	Apart
T4293	11835-11839	IN	denotes	from
T4294	11840-11845	DT	denotes	these
T4296	11846-11854	JJ	denotes	efferent
T4295	11855-11863	NNS	denotes	neurites
T4297	11864-11867	DT	denotes	the
T4299	11868-11875	NNS	denotes	laminae
T4298	11876-11886	NNS	denotes	medullares
T4300	11887-11888	-LRB-	denotes	(
T4302	11888-11894	NN	denotes	lamina
T4303	11895-11899	NN	denotes	alba
T4304	11899-11901	,	denotes	,
T4305	11901-11906	JJ	denotes	white
T4301	11907-11913	NN	denotes	matter
T4306	11913-11914	-RRB-	denotes	)
T4292	11915-11922	VBP	denotes	contain
T4307	11923-11931	JJ	denotes	afferent
T4309	11932-11937	JJ	denotes	mossy
T4310	11938-11941	CC	denotes	and
T4311	11942-11950	VBG	denotes	climbing
T4308	11951-11957	NNS	denotes	fibers
T4312	11957-11958	.	denotes	.
T4313	11958-12072	sentence	denotes	Thus, the intense stain of the cerebellar white matter arises from Annexin A7 located in astrocytes and neurites.
T4314	11959-11963	RB	denotes	Thus
T4316	11963-11965	,	denotes	,
T4317	11965-11968	DT	denotes	the
T4319	11969-11976	JJ	denotes	intense
T4318	11977-11982	NN	denotes	stain
T4320	11983-11985	IN	denotes	of
T4321	11986-11989	DT	denotes	the
T4323	11990-12000	JJ	denotes	cerebellar
T4324	12001-12006	JJ	denotes	white
T4322	12007-12013	NN	denotes	matter
T4315	12014-12020	VBZ	denotes	arises
T4325	12021-12025	IN	denotes	from
T4326	12026-12033	NN	denotes	Annexin
T4327	12034-12036	NN	denotes	A7
T4328	12037-12044	VBN	denotes	located
T4329	12045-12047	IN	denotes	in
T4330	12048-12058	NNS	denotes	astrocytes
T4331	12059-12062	CC	denotes	and
T4332	12063-12071	NNS	denotes	neurites
T4333	12071-12072	.	denotes	.
T14188	12083-12090	NN	denotes	Annexin
T14189	12091-12093	NN	denotes	A7
T14190	12094-12108	NN	denotes	immunostaining
T14191	12109-12111	IN	denotes	in
T14192	12112-12115	DT	denotes	the
T14193	12116-12126	NN	denotes	cerebellum
T14194	12127-12129	IN	denotes	of
T14195	12130-12135	JJ	denotes	adult
T14196	12136-12140	NNS	denotes	mice
T14197	12140-12141	.	denotes	.
T14198	12141-12281	sentence	denotes	(A) Low magnification of the cerebellum presents an Annexin A7 expression mainly in cells of the stratum granulosum and laminae medullares.
T14199	12142-12143	-LRB-	denotes	(
T14200	12143-12144	LS	denotes	A
T14202	12144-12145	-RRB-	denotes	)
T14203	12146-12149	JJ	denotes	Low
T14204	12150-12163	NN	denotes	magnification
T14205	12164-12166	IN	denotes	of
T14206	12167-12170	DT	denotes	the
T14207	12171-12181	NN	denotes	cerebellum
T14201	12182-12190	VBZ	denotes	presents
T14208	12191-12193	DT	denotes	an
T14210	12194-12201	NN	denotes	Annexin
T14211	12202-12204	NN	denotes	A7
T14209	12205-12215	NN	denotes	expression
T14212	12216-12222	RB	denotes	mainly
T14213	12223-12225	IN	denotes	in
T14214	12226-12231	NNS	denotes	cells
T14215	12232-12234	IN	denotes	of
T14216	12235-12238	DT	denotes	the
T14218	12239-12246	NN	denotes	stratum
T14217	12247-12257	NN	denotes	granulosum
T14219	12258-12261	CC	denotes	and
T14220	12262-12269	NNS	denotes	laminae
T14221	12270-12280	NNS	denotes	medullares
T14222	12280-12281	.	denotes	.
T14223	12281-12325	sentence	denotes	(B) Corresponding section stained for GFAP.
T14224	12282-12283	-LRB-	denotes	(
T14225	12283-12284	LS	denotes	B
T14227	12284-12285	-RRB-	denotes	)
T14228	12286-12299	JJ	denotes	Corresponding
T14226	12300-12307	NN	denotes	section
T14229	12308-12315	VBN	denotes	stained
T14230	12316-12319	IN	denotes	for
T14231	12320-12324	NN	denotes	GFAP
T14232	12324-12325	.	denotes	.
T14233	12325-12527	sentence	denotes	(C) Higher magnification of folia of the cerebellum, where a polyclonal anti-AnnexinA7 antibody was used; square, a higher magnification of acorresponding area stained with mAb 203–217 is given in (D).
T14234	12326-12327	-LRB-	denotes	(
T14235	12327-12328	LS	denotes	C
T14237	12328-12329	-RRB-	denotes	)
T14238	12330-12336	JJR	denotes	Higher
T14236	12337-12350	NN	denotes	magnification
T14239	12351-12353	IN	denotes	of
T14240	12354-12359	NNS	denotes	folia
T14241	12360-12362	IN	denotes	of
T14242	12363-12366	DT	denotes	the
T14243	12367-12377	NN	denotes	cerebellum
T14244	12377-12379	,	denotes	,
T14245	12379-12384	WRB	denotes	where
T14247	12385-12386	DT	denotes	a
T14249	12387-12397	JJ	denotes	polyclonal
T14250	12398-12412	JJ	denotes	anti-AnnexinA7
T14248	12413-12421	NN	denotes	antibody
T14251	12422-12425	VBD	denotes	was
T14246	12426-12430	VBN	denotes	used
T14252	12430-12431	:	denotes	;
T14253	12432-12438	NN	denotes	square
T14255	12438-12440	,	denotes	,
T14256	12440-12441	DT	denotes	a
T14258	12442-12448	JJR	denotes	higher
T14257	12449-12462	NN	denotes	magnification
T14259	12463-12465	IN	denotes	of
T14260	12466-12480	JJ	denotes	acorresponding
T14261	12481-12485	NN	denotes	area
T14262	12486-12493	VBN	denotes	stained
T14263	12494-12498	IN	denotes	with
T14264	12499-12502	NN	denotes	mAb
T14265	12503-12506	CD	denotes	203
T14266	12506-12507	SYM	denotes	–
T14267	12507-12510	CD	denotes	217
T14268	12511-12513	VBZ	denotes	is
T14254	12514-12519	VBN	denotes	given
T14269	12520-12522	IN	denotes	in
T14270	12523-12524	-LRB-	denotes	(
T14271	12524-12525	NN	denotes	D
T14272	12525-12526	-RRB-	denotes	)
T14273	12526-12527	.	denotes	.
T14274	12527-12669	sentence	denotes	Between stratum granulosum and stratum moleculare the layer of Purkinje-cells (stratum neuronorum piriformium (ganglionare)) can be observed.
T14275	12528-12535	IN	denotes	Between
T14277	12536-12543	NN	denotes	stratum
T14278	12544-12554	NN	denotes	granulosum
T14279	12555-12558	CC	denotes	and
T14280	12559-12566	NN	denotes	stratum
T14281	12567-12577	NN	denotes	moleculare
T14282	12578-12581	DT	denotes	the
T14283	12582-12587	NN	denotes	layer
T14284	12588-12590	IN	denotes	of
T14285	12591-12599	NNP	denotes	Purkinje
T14287	12599-12600	HYPH	denotes	-
T14286	12600-12605	NNS	denotes	cells
T14288	12606-12607	-LRB-	denotes	(
T14290	12607-12614	NN	denotes	stratum
T14291	12615-12625	NN	denotes	neuronorum
T14289	12626-12637	NN	denotes	piriformium
T14292	12638-12639	-LRB-	denotes	(
T14293	12639-12650	NN	denotes	ganglionare
T14294	12650-12651	-RRB-	denotes	)
T14295	12651-12652	-RRB-	denotes	)
T14296	12653-12656	MD	denotes	can
T14297	12657-12659	VB	denotes	be
T14276	12660-12668	VBN	denotes	observed
T14298	12668-12669	.	denotes	.
T14299	12669-12722	sentence	denotes	(D) Staining of the band of Purkinje-cells (arrows).
T14300	12670-12671	-LRB-	denotes	(
T14301	12671-12672	LS	denotes	D
T14303	12672-12673	-RRB-	denotes	)
T14302	12674-12682	NN	denotes	Staining
T14304	12683-12685	IN	denotes	of
T14305	12686-12689	DT	denotes	the
T14306	12690-12694	NN	denotes	band
T14307	12695-12697	IN	denotes	of
T14308	12698-12706	NNP	denotes	Purkinje
T14310	12706-12707	HYPH	denotes	-
T14309	12707-12712	NNS	denotes	cells
T14311	12713-12714	-LRB-	denotes	(
T14312	12714-12720	NNS	denotes	arrows
T14313	12720-12721	-RRB-	denotes	)
T14314	12721-12722	.	denotes	.
T14315	12722-12823	sentence	denotes	The positive Annexin A7-stain in the stratum granulosum is due to staining of the nuclei of neurons.
T14316	12723-12726	DT	denotes	The
T14318	12727-12735	JJ	denotes	positive
T14319	12736-12743	NN	denotes	Annexin
T14320	12744-12746	NN	denotes	A7
T14321	12746-12747	HYPH	denotes	-
T14317	12747-12752	NN	denotes	stain
T14323	12753-12755	IN	denotes	in
T14324	12756-12759	DT	denotes	the
T14326	12760-12767	NN	denotes	stratum
T14325	12768-12778	NN	denotes	granulosum
T14322	12779-12781	VBZ	denotes	is
T14327	12782-12785	IN	denotes	due
T14328	12786-12788	IN	denotes	to
T14329	12789-12797	NN	denotes	staining
T14330	12798-12800	IN	denotes	of
T14331	12801-12804	DT	denotes	the
T14332	12805-12811	NNS	denotes	nuclei
T14333	12812-12814	IN	denotes	of
T14334	12815-12822	NNS	denotes	neurons
T14335	12822-12823	.	denotes	.
T14336	12823-12967	sentence	denotes	(E) In addition to an intense staining of the nucleus, the cell body is AnnexinA7-positive including both dendrites of the Purkinje-cell shown.
T14337	12824-12825	-LRB-	denotes	(
T14338	12825-12826	LS	denotes	E
T14340	12826-12827	-RRB-	denotes	)
T14341	12828-12830	IN	denotes	In
T14342	12831-12839	NN	denotes	addition
T14343	12840-12842	IN	denotes	to
T14344	12843-12845	DT	denotes	an
T14346	12846-12853	JJ	denotes	intense
T14345	12854-12862	NN	denotes	staining
T14347	12863-12865	IN	denotes	of
T14348	12866-12869	DT	denotes	the
T14349	12870-12877	NN	denotes	nucleus
T14350	12877-12879	,	denotes	,
T14351	12879-12882	DT	denotes	the
T14353	12883-12887	NN	denotes	cell
T14352	12888-12892	NN	denotes	body
T14339	12893-12895	VBZ	denotes	is
T14354	12896-12905	NN	denotes	AnnexinA7
T14356	12905-12906	HYPH	denotes	-
T14355	12906-12914	JJ	denotes	positive
T14357	12915-12924	VBG	denotes	including
T14358	12925-12929	DT	denotes	both
T14359	12930-12939	NNS	denotes	dendrites
T14360	12940-12942	IN	denotes	of
T14361	12943-12946	DT	denotes	the
T14363	12947-12955	NNP	denotes	Purkinje
T14364	12955-12956	HYPH	denotes	-
T14362	12956-12960	NN	denotes	cell
T14365	12961-12966	VBN	denotes	shown
T14366	12966-12967	.	denotes	.
T14367	12967-13017	sentence	denotes	(F) Corresponding section from an AnxA7-/- mouse.
T14368	12968-12969	-LRB-	denotes	(
T14369	12969-12970	LS	denotes	F
T14371	12970-12971	-RRB-	denotes	)
T14372	12972-12985	JJ	denotes	Corresponding
T14370	12986-12993	NN	denotes	section
T14373	12994-12998	IN	denotes	from
T14374	12999-13001	DT	denotes	an
T14376	13002-13007	NN	denotes	AnxA7
T14377	13007-13008	SYM	denotes	-
T14378	13008-13009	HYPH	denotes	/
T14379	13009-13010	SYM	denotes	-
T14375	13011-13016	NN	denotes	mouse
T14380	13016-13017	.	denotes	.
T14381	13017-13168	sentence	denotes	(G) AnnexinA7 staining of axons (arrowheads) running from the laminae medullares to the Purkinje-cell layer located in the round end of a convolution.
T14382	13018-13019	-LRB-	denotes	(
T14383	13019-13020	LS	denotes	G
T14385	13020-13021	-RRB-	denotes	)
T14386	13022-13031	NN	denotes	AnnexinA7
T14384	13032-13040	NN	denotes	staining
T14387	13041-13043	IN	denotes	of
T14388	13044-13049	NNS	denotes	axons
T14389	13050-13051	-LRB-	denotes	(
T14390	13051-13061	NNS	denotes	arrowheads
T14391	13061-13062	-RRB-	denotes	)
T14392	13063-13070	VBG	denotes	running
T14393	13071-13075	IN	denotes	from
T14394	13076-13079	DT	denotes	the
T14396	13080-13087	NNS	denotes	laminae
T14395	13088-13098	NNS	denotes	medullares
T14397	13099-13101	IN	denotes	to
T14398	13102-13105	DT	denotes	the
T14400	13106-13114	NNP	denotes	Purkinje
T14402	13114-13115	HYPH	denotes	-
T14401	13115-13119	NN	denotes	cell
T14399	13120-13125	NN	denotes	layer
T14403	13126-13133	VBN	denotes	located
T14404	13134-13136	IN	denotes	in
T14405	13137-13140	DT	denotes	the
T14407	13141-13146	JJ	denotes	round
T14406	13147-13150	NN	denotes	end
T14408	13151-13153	IN	denotes	of
T14409	13154-13155	DT	denotes	a
T14410	13156-13167	NN	denotes	convolution
T14411	13167-13168	.	denotes	.
T14412	13168-13222	sentence	denotes	Sections A, D, E, F, G were stained with mAb 203–217.
T14413	13169-13177	NNS	denotes	Sections
T14415	13178-13179	NN	denotes	A
T14416	13179-13181	,	denotes	,
T14417	13181-13182	NN	denotes	D
T14418	13182-13184	,	denotes	,
T14419	13184-13185	NN	denotes	E
T14420	13185-13187	,	denotes	,
T14421	13187-13188	NN	denotes	F
T14422	13188-13190	,	denotes	,
T14414	13190-13191	NN	denotes	G
T14424	13192-13196	VBD	denotes	were
T14423	13197-13204	VBN	denotes	stained
T14425	13205-13209	IN	denotes	with
T14426	13210-13213	NN	denotes	mAb
T14427	13214-13217	CD	denotes	203
T14428	13217-13218	SYM	denotes	–
T14429	13218-13221	CD	denotes	217
T14430	13221-13222	.	denotes	.
T4422	13224-13234	NN	denotes	Expression
T4423	13235-13237	IN	denotes	of
T4424	13238-13245	NN	denotes	Annexin
T4425	13246-13248	NN	denotes	A7
T4426	13249-13251	IN	denotes	in
T4427	13252-13255	DT	denotes	the
T4429	13256-13261	JJ	denotes	adult
T4430	13262-13267	JJ	denotes	human
T4428	13268-13277	NN	denotes	isocortex
T4431	13277-13444	sentence	denotes	In the human parietal neocortex of aged individuals without any neuropathological alterations, subpial astrocytes exhibited a staining of Annexin A7 in the cytoplasm.
T4432	13278-13280	IN	denotes	In
T4434	13281-13284	DT	denotes	the
T4436	13285-13290	JJ	denotes	human
T4437	13291-13299	JJ	denotes	parietal
T4435	13300-13309	NN	denotes	neocortex
T4438	13310-13312	IN	denotes	of
T4439	13313-13317	JJ	denotes	aged
T4440	13318-13329	NNS	denotes	individuals
T4441	13330-13337	IN	denotes	without
T4442	13338-13341	DT	denotes	any
T4444	13342-13359	JJ	denotes	neuropathological
T4443	13360-13371	NNS	denotes	alterations
T4445	13371-13373	,	denotes	,
T4446	13373-13380	JJ	denotes	subpial
T4447	13381-13391	NNS	denotes	astrocytes
T4433	13392-13401	VBD	denotes	exhibited
T4448	13402-13403	DT	denotes	a
T4449	13404-13412	NN	denotes	staining
T4450	13413-13415	IN	denotes	of
T4451	13416-13423	NN	denotes	Annexin
T4452	13424-13426	NN	denotes	A7
T4453	13427-13429	IN	denotes	in
T4454	13430-13433	DT	denotes	the
T4455	13434-13443	NN	denotes	cytoplasm
T4456	13443-13444	.	denotes	.
T4457	13444-13521	sentence	denotes	A nuclear presence of Annexin A7 was limited to single astrocytes (Fig. 6B).
T4458	13445-13446	DT	denotes	A
T4460	13447-13454	JJ	denotes	nuclear
T4459	13455-13463	NN	denotes	presence
T4462	13464-13466	IN	denotes	of
T4463	13467-13474	NN	denotes	Annexin
T4464	13475-13477	NN	denotes	A7
T4465	13478-13481	VBD	denotes	was
T4461	13482-13489	VBN	denotes	limited
T4466	13490-13492	IN	denotes	to
T4467	13493-13499	JJ	denotes	single
T4468	13500-13510	NNS	denotes	astrocytes
T4469	13511-13512	-LRB-	denotes	(
T4471	13512-13516	NN	denotes	Fig.
T4470	13517-13519	NN	denotes	6B
T4472	13519-13520	-RRB-	denotes	)
T4473	13520-13521	.	denotes	.
T4474	13521-13682	sentence	denotes	Pyramidal neurons, predominantly those of layer V exhibited Annexin A7 at the plasma membrane of their perikaryon as well as of the apical dendrite (Fig. 6A,C).
T4475	13522-13531	JJ	denotes	Pyramidal
T4476	13532-13539	NNS	denotes	neurons
T4478	13539-13541	,	denotes	,
T4479	13541-13554	RB	denotes	predominantly
T4480	13555-13560	DT	denotes	those
T4481	13561-13563	IN	denotes	of
T4482	13564-13569	NN	denotes	layer
T4483	13570-13571	CD	denotes	V
T4477	13572-13581	VBD	denotes	exhibited
T4484	13582-13589	NN	denotes	Annexin
T4485	13590-13592	NN	denotes	A7
T4486	13593-13595	IN	denotes	at
T4487	13596-13599	DT	denotes	the
T4489	13600-13606	NN	denotes	plasma
T4488	13607-13615	NN	denotes	membrane
T4490	13616-13618	IN	denotes	of
T4491	13619-13624	PRP$	denotes	their
T4492	13625-13635	NN	denotes	perikaryon
T4493	13636-13638	RB	denotes	as
T4495	13639-13643	RB	denotes	well
T4494	13644-13646	IN	denotes	as
T4496	13647-13649	IN	denotes	of
T4497	13650-13653	DT	denotes	the
T4499	13654-13660	JJ	denotes	apical
T4498	13661-13669	NN	denotes	dendrite
T4500	13670-13671	-LRB-	denotes	(
T4502	13671-13675	NN	denotes	Fig.
T4503	13676-13678	NN	denotes	6A
T4504	13678-13679	,	denotes	,
T4501	13679-13680	NN	denotes	C
T4505	13680-13681	-RRB-	denotes	)
T4506	13681-13682	.	denotes	.
T4507	13682-13742	sentence	denotes	The neurons lacked a signal for Annexin A7 in their nuclei.
T4508	13683-13686	DT	denotes	The
T4509	13687-13694	NNS	denotes	neurons
T4510	13695-13701	VBD	denotes	lacked
T4511	13702-13703	DT	denotes	a
T4512	13704-13710	NN	denotes	signal
T4513	13711-13714	IN	denotes	for
T4514	13715-13722	NN	denotes	Annexin
T4515	13723-13725	NN	denotes	A7
T4516	13726-13728	IN	denotes	in
T4517	13729-13734	PRP$	denotes	their
T4518	13735-13741	NNS	denotes	nuclei
T4519	13741-13742	.	denotes	.
T4520	13742-13847	sentence	denotes	Apical dendrites within the molecular layer also indicated a positive staining for Annexin A7 (Fig. 6B).
T4521	13743-13749	JJ	denotes	Apical
T4522	13750-13759	NNS	denotes	dendrites
T4524	13760-13766	IN	denotes	within
T4525	13767-13770	DT	denotes	the
T4527	13771-13780	JJ	denotes	molecular
T4526	13781-13786	NN	denotes	layer
T4528	13787-13791	RB	denotes	also
T4523	13792-13801	VBD	denotes	indicated
T4529	13802-13803	DT	denotes	a
T4531	13804-13812	JJ	denotes	positive
T4530	13813-13821	NN	denotes	staining
T4532	13822-13825	IN	denotes	for
T4533	13826-13833	NN	denotes	Annexin
T4534	13834-13836	NN	denotes	A7
T4535	13837-13838	-LRB-	denotes	(
T4537	13838-13842	NN	denotes	Fig.
T4536	13843-13845	NN	denotes	6B
T4538	13845-13846	-RRB-	denotes	)
T4539	13846-13847	.	denotes	.
T4540	13847-13958	sentence	denotes	The staining pattern of Annexin A7 in the human autopsy brain did not change after pre-treatment with trypsin.
T4541	13848-13851	DT	denotes	The
T4543	13852-13860	NN	denotes	staining
T4542	13861-13868	NN	denotes	pattern
T4545	13869-13871	IN	denotes	of
T4546	13872-13879	NN	denotes	Annexin
T4547	13880-13882	NN	denotes	A7
T4548	13883-13885	IN	denotes	in
T4549	13886-13889	DT	denotes	the
T4551	13890-13895	JJ	denotes	human
T4552	13896-13903	NN	denotes	autopsy
T4550	13904-13909	NN	denotes	brain
T4553	13910-13913	VBD	denotes	did
T4554	13914-13917	RB	denotes	not
T4544	13918-13924	VB	denotes	change
T4555	13925-13930	IN	denotes	after
T4556	13931-13944	NN	denotes	pre-treatment
T4557	13945-13949	IN	denotes	with
T4558	13950-13957	NN	denotes	trypsin
T4559	13957-13958	.	denotes	.
T14500	13969-13976	NN	denotes	Annexin
T14501	13977-13979	NN	denotes	A7
T14502	13980-13994	NN	denotes	immunostaining
T14503	13995-13997	IN	denotes	in
T14504	13998-14003	JJ	denotes	human
T14505	14004-14013	NN	denotes	isocortex
T14506	14013-14014	.	denotes	.
T14507	14014-14173	sentence	denotes	(A) Pyramidal neurons (bold arrow) and apical dendrites (small arrows) were clearly labeled with the antibody against Annexin A7 in the human parietal cortex.
T14508	14015-14016	-LRB-	denotes	(
T14509	14016-14017	LS	denotes	A
T14511	14017-14018	-RRB-	denotes	)
T14512	14019-14028	JJ	denotes	Pyramidal
T14513	14029-14036	NNS	denotes	neurons
T14514	14037-14038	-LRB-	denotes	(
T14516	14038-14042	JJ	denotes	bold
T14515	14043-14048	NN	denotes	arrow
T14517	14048-14049	-RRB-	denotes	)
T14518	14050-14053	CC	denotes	and
T14519	14054-14060	JJ	denotes	apical
T14520	14061-14070	NNS	denotes	dendrites
T14521	14071-14072	-LRB-	denotes	(
T14523	14072-14077	JJ	denotes	small
T14522	14078-14084	NNS	denotes	arrows
T14524	14084-14085	-RRB-	denotes	)
T14525	14086-14090	VBD	denotes	were
T14526	14091-14098	RB	denotes	clearly
T14510	14099-14106	VBN	denotes	labeled
T14527	14107-14111	IN	denotes	with
T14528	14112-14115	DT	denotes	the
T14529	14116-14124	NN	denotes	antibody
T14530	14125-14132	IN	denotes	against
T14531	14133-14140	NN	denotes	Annexin
T14532	14141-14143	NN	denotes	A7
T14533	14144-14146	IN	denotes	in
T14534	14147-14150	DT	denotes	the
T14536	14151-14156	JJ	denotes	human
T14537	14157-14165	JJ	denotes	parietal
T14535	14166-14172	NN	denotes	cortex
T14538	14172-14173	.	denotes	.
T14539	14173-14251	sentence	denotes	(B) A dendritic staining was also seen in the molecular layer (small arrows).
T14540	14174-14175	-LRB-	denotes	(
T14541	14175-14176	LS	denotes	B
T14543	14176-14177	-RRB-	denotes	)
T14544	14178-14179	DT	denotes	A
T14546	14180-14189	JJ	denotes	dendritic
T14545	14190-14198	NN	denotes	staining
T14547	14199-14202	VBD	denotes	was
T14548	14203-14207	RB	denotes	also
T14542	14208-14212	VBN	denotes	seen
T14549	14213-14215	IN	denotes	in
T14550	14216-14219	DT	denotes	the
T14552	14220-14229	JJ	denotes	molecular
T14551	14230-14235	NN	denotes	layer
T14553	14236-14237	-LRB-	denotes	(
T14555	14237-14242	JJ	denotes	small
T14554	14243-14249	NNS	denotes	arrows
T14556	14249-14250	-RRB-	denotes	)
T14557	14250-14251	.	denotes	.
T14558	14251-14330	sentence	denotes	In addition the subpial astrocytes were weakly labeled (double headed arrows).
T14559	14252-14254	IN	denotes	In
T14561	14255-14263	NN	denotes	addition
T14562	14264-14267	DT	denotes	the
T14564	14268-14275	JJ	denotes	subpial
T14563	14276-14286	NNS	denotes	astrocytes
T14565	14287-14291	VBD	denotes	were
T14566	14292-14298	RB	denotes	weakly
T14560	14299-14306	VBN	denotes	labeled
T14567	14307-14308	-LRB-	denotes	(
T14569	14308-14314	JJ	denotes	double
T14570	14315-14321	JJ	denotes	headed
T14568	14322-14328	NNS	denotes	arrows
T14571	14328-14329	-RRB-	denotes	)
T14572	14329-14330	.	denotes	.
T14573	14330-14423	sentence	denotes	The staining was cytoplasmic, only few astrocytes showed a nuclear staining as well (inset).
T14574	14331-14334	DT	denotes	The
T14575	14335-14343	NN	denotes	staining
T14576	14344-14347	VBD	denotes	was
T14578	14348-14359	JJ	denotes	cytoplasmic
T14579	14359-14361	,	denotes	,
T14580	14361-14365	RB	denotes	only
T14582	14366-14369	JJ	denotes	few
T14581	14370-14380	NNS	denotes	astrocytes
T14577	14381-14387	VBD	denotes	showed
T14583	14388-14389	DT	denotes	a
T14585	14390-14397	JJ	denotes	nuclear
T14584	14398-14406	NN	denotes	staining
T14586	14407-14409	RB	denotes	as
T14587	14410-14414	RB	denotes	well
T14588	14415-14416	-LRB-	denotes	(
T14589	14416-14421	NN	denotes	inset
T14590	14421-14422	-RRB-	denotes	)
T14591	14422-14423	.	denotes	.
T14592	14423-14559	sentence	denotes	(C) Enlargement of (A): Annexin A7 was seen at the cell membrane of the perikaryon (bold arrow) and the apical dendrite (small arrows).
T14593	14424-14425	-LRB-	denotes	(
T14594	14425-14426	LS	denotes	C
T14596	14426-14427	-RRB-	denotes	)
T14597	14428-14439	NN	denotes	Enlargement
T14598	14440-14442	IN	denotes	of
T14599	14443-14444	-LRB-	denotes	(
T14600	14444-14445	NN	denotes	A
T14601	14445-14446	-RRB-	denotes	)
T14602	14446-14448	:	denotes	:
T14603	14448-14455	NN	denotes	Annexin
T14604	14456-14458	NN	denotes	A7
T14605	14459-14462	VBD	denotes	was
T14595	14463-14467	VBN	denotes	seen
T14606	14468-14470	IN	denotes	at
T14607	14471-14474	DT	denotes	the
T14609	14475-14479	NN	denotes	cell
T14608	14480-14488	NN	denotes	membrane
T14610	14489-14491	IN	denotes	of
T14611	14492-14495	DT	denotes	the
T14612	14496-14506	NN	denotes	perikaryon
T14613	14507-14508	-LRB-	denotes	(
T14615	14508-14512	JJ	denotes	bold
T14614	14513-14518	NN	denotes	arrow
T14616	14518-14519	-RRB-	denotes	)
T14617	14520-14523	CC	denotes	and
T14618	14524-14527	DT	denotes	the
T14620	14528-14534	JJ	denotes	apical
T14619	14535-14543	NN	denotes	dendrite
T14621	14544-14545	-LRB-	denotes	(
T14623	14545-14550	JJ	denotes	small
T14622	14551-14557	NNS	denotes	arrows
T14624	14557-14558	-RRB-	denotes	)
T14625	14558-14559	.	denotes	.
T14626	14559-14717	sentence	denotes	(D) A blank control lacking the primary antibody did not show a specific labeling, but autofluorescent lipofuscin was detectable in the neurons (arrowheads).
T14627	14560-14561	-LRB-	denotes	(
T14628	14561-14562	LS	denotes	D
T14630	14562-14563	-RRB-	denotes	)
T14631	14564-14565	DT	denotes	A
T14633	14566-14571	JJ	denotes	blank
T14632	14572-14579	NN	denotes	control
T14634	14580-14587	VBG	denotes	lacking
T14635	14588-14591	DT	denotes	the
T14637	14592-14599	JJ	denotes	primary
T14636	14600-14608	NN	denotes	antibody
T14638	14609-14612	VBD	denotes	did
T14639	14613-14616	RB	denotes	not
T14629	14617-14621	VB	denotes	show
T14640	14622-14623	DT	denotes	a
T14642	14624-14632	JJ	denotes	specific
T14641	14633-14641	NN	denotes	labeling
T14643	14641-14643	,	denotes	,
T14644	14643-14646	CC	denotes	but
T14645	14647-14662	JJ	denotes	autofluorescent
T14646	14663-14673	NN	denotes	lipofuscin
T14647	14674-14677	VBD	denotes	was
T14648	14678-14688	JJ	denotes	detectable
T14649	14689-14691	IN	denotes	in
T14650	14692-14695	DT	denotes	the
T14651	14696-14703	NNS	denotes	neurons
T14652	14704-14705	-LRB-	denotes	(
T14653	14705-14715	NNS	denotes	arrowheads
T14654	14715-14716	-RRB-	denotes	)
T14655	14716-14717	.	denotes	.
T14656	14717-14775	sentence	denotes	Bar, (A) 70 μm, (B) 50 μm, (B-inset, C) 17 μm, (D) 20 μm.
T14657	14718-14721	NN	denotes	Bar
T14658	14721-14723	,	denotes	,
T14659	14723-14724	-LRB-	denotes	(
T14660	14724-14725	LS	denotes	A
T14662	14725-14726	-RRB-	denotes	)
T14663	14727-14729	CD	denotes	70
T14661	14730-14732	NN	denotes	μm
T14664	14732-14734	,	denotes	,
T14665	14734-14735	-LRB-	denotes	(
T14666	14735-14736	LS	denotes	B
T14668	14736-14737	-RRB-	denotes	)
T14669	14738-14740	CD	denotes	50
T14667	14741-14743	NN	denotes	μm
T14670	14743-14745	,	denotes	,
T14671	14745-14746	-LRB-	denotes	(
T14672	14746-14747	LS	denotes	B
T14674	14747-14748	HYPH	denotes	-
T14675	14748-14753	NN	denotes	inset
T14676	14753-14755	,	denotes	,
T14677	14755-14756	LS	denotes	C
T14678	14756-14757	-RRB-	denotes	)
T14679	14758-14760	CD	denotes	17
T14673	14761-14763	NN	denotes	μm
T14680	14763-14765	,	denotes	,
T14681	14765-14766	-LRB-	denotes	(
T14682	14766-14767	LS	denotes	D
T14684	14767-14768	-RRB-	denotes	)
T14685	14769-14771	CD	denotes	20
T14683	14772-14774	NN	denotes	μm
T14686	14774-14775	.	denotes	.
T5136	14777-14785	NN	denotes	Presence
T5137	14786-14788	IN	denotes	of
T5138	14789-14796	NN	denotes	Annexin
T5139	14797-14799	NN	denotes	A7
T5140	14800-14802	IN	denotes	in
T5141	14803-14809	NNS	denotes	nuclei
T5142	14810-14814	IN	denotes	from
T5143	14815-14823	JJ	denotes	neuronal
T5145	14824-14827	CC	denotes	and
T5146	14828-14838	JJ	denotes	astroglial
T5144	14839-14844	NNS	denotes	cells
T5147	14844-15080	sentence	denotes	The nuclear localisation of Annexin A7 in mice observed in immature cells at E16 and differentiated adult neurons could be only detected as a strong signal when the brain sections were pre-treated with trypsin before antibody staining.
T5148	14845-14848	DT	denotes	The
T5150	14849-14856	JJ	denotes	nuclear
T5149	14857-14869	NN	denotes	localisation
T5152	14870-14872	IN	denotes	of
T5153	14873-14880	NN	denotes	Annexin
T5154	14881-14883	NN	denotes	A7
T5155	14884-14886	IN	denotes	in
T5156	14887-14891	NNS	denotes	mice
T5157	14892-14900	VBN	denotes	observed
T5158	14901-14903	IN	denotes	in
T5159	14904-14912	JJ	denotes	immature
T5160	14913-14918	NNS	denotes	cells
T5161	14919-14921	IN	denotes	at
T5162	14922-14925	NN	denotes	E16
T5163	14926-14929	CC	denotes	and
T5164	14930-14944	VBN	denotes	differentiated
T5166	14945-14950	JJ	denotes	adult
T5165	14951-14958	NNS	denotes	neurons
T5167	14959-14964	MD	denotes	could
T5168	14965-14967	VB	denotes	be
T5169	14968-14972	RB	denotes	only
T5151	14973-14981	VBN	denotes	detected
T5170	14982-14984	IN	denotes	as
T5171	14985-14986	DT	denotes	a
T5173	14987-14993	JJ	denotes	strong
T5172	14994-15000	NN	denotes	signal
T5174	15001-15005	WRB	denotes	when
T5176	15006-15009	DT	denotes	the
T5178	15010-15015	NN	denotes	brain
T5177	15016-15024	NNS	denotes	sections
T5179	15025-15029	VBD	denotes	were
T5175	15030-15041	VBN	denotes	pre-treated
T5180	15042-15046	IN	denotes	with
T5181	15047-15054	NN	denotes	trypsin
T5182	15055-15061	IN	denotes	before
T5183	15062-15070	NN	denotes	antibody
T5184	15071-15079	NN	denotes	staining
T5185	15079-15080	.	denotes	.
T5186	15080-15186	sentence	denotes	This procedure is thought to allow the antibodies to access epitopes masked by the formaldehyde fixation.
T5187	15081-15085	DT	denotes	This
T5188	15086-15095	NN	denotes	procedure
T5190	15096-15098	VBZ	denotes	is
T5189	15099-15106	VBN	denotes	thought
T5191	15107-15109	TO	denotes	to
T5192	15110-15115	VB	denotes	allow
T5193	15116-15119	DT	denotes	the
T5194	15120-15130	NNS	denotes	antibodies
T5196	15131-15133	TO	denotes	to
T5195	15134-15140	VB	denotes	access
T5197	15141-15149	NNS	denotes	epitopes
T5198	15150-15156	VBN	denotes	masked
T5199	15157-15159	IN	denotes	by
T5200	15160-15163	DT	denotes	the
T5202	15164-15176	NN	denotes	formaldehyde
T5201	15177-15185	NN	denotes	fixation
T5203	15185-15186	.	denotes	.
T5204	15186-15251	sentence	denotes	Methanol fixation of cultured cells led to inconsistent results.
T5205	15187-15195	NN	denotes	Methanol
T5206	15196-15204	NN	denotes	fixation
T5208	15205-15207	IN	denotes	of
T5209	15208-15216	VBN	denotes	cultured
T5210	15217-15222	NNS	denotes	cells
T5207	15223-15226	VBD	denotes	led
T5211	15227-15229	IN	denotes	to
T5212	15230-15242	JJ	denotes	inconsistent
T5213	15243-15250	NNS	denotes	results
T5214	15250-15251	.	denotes	.
T5215	15251-15462	sentence	denotes	Antigen retrieval in formalin-fixed and paraffin-embedded tissue sections employs various heating or proteolytic pre-treatment methods [[15-17]; Ein Handbuch für die Histologie, dianova GmbH, Hamburg, Germany].
T5216	15252-15259	NN	denotes	Antigen
T5217	15260-15269	NN	denotes	retrieval
T5219	15270-15272	IN	denotes	in
T5220	15273-15281	NN	denotes	formalin
T5222	15281-15282	HYPH	denotes	-
T5221	15282-15287	VBN	denotes	fixed
T5224	15288-15291	CC	denotes	and
T5225	15292-15300	NN	denotes	paraffin
T5227	15300-15301	HYPH	denotes	-
T5226	15301-15309	VBN	denotes	embedded
T5228	15310-15316	NN	denotes	tissue
T5223	15317-15325	NNS	denotes	sections
T5218	15326-15333	VBZ	denotes	employs
T5229	15334-15341	JJ	denotes	various
T5231	15342-15349	NN	denotes	heating
T5232	15350-15352	CC	denotes	or
T5233	15353-15364	JJ	denotes	proteolytic
T5234	15365-15378	NN	denotes	pre-treatment
T5230	15379-15386	NNS	denotes	methods
T5235	15387-15388	-LRB-	denotes	[
T5236	15388-15389	-LRB-	denotes	[
T5237	15389-15391	CD	denotes	15
T5238	15391-15392	SYM	denotes	-
T5239	15392-15394	CD	denotes	17
T5240	15394-15395	-RRB-	denotes	]
T5241	15395-15396	:	denotes	;
T5242	15397-15400	NNP	denotes	Ein
T5243	15401-15409	NNP	denotes	Handbuch
T5244	15410-15413	NNP	denotes	für
T5245	15414-15417	NNP	denotes	die
T5246	15418-15428	NNP	denotes	Histologie
T5247	15428-15430	,	denotes	,
T5248	15430-15437	NNP	denotes	dianova
T5249	15438-15442	NNP	denotes	GmbH
T5250	15442-15444	,	denotes	,
T5251	15444-15451	NNP	denotes	Hamburg
T5252	15451-15453	,	denotes	,
T5253	15453-15460	NNP	denotes	Germany
T5254	15460-15461	-RRB-	denotes	]
T5255	15461-15462	.	denotes	.
T5256	15462-15597	sentence	denotes	These methods can result in moderate or strong specific antibody staining, but the detectability of other antigens might be decreased.
T5257	15463-15468	DT	denotes	These
T5258	15469-15476	NNS	denotes	methods
T5260	15477-15480	MD	denotes	can
T5259	15481-15487	VB	denotes	result
T5261	15488-15490	IN	denotes	in
T5262	15491-15499	JJ	denotes	moderate
T5264	15500-15502	CC	denotes	or
T5265	15503-15509	JJ	denotes	strong
T5266	15510-15518	JJ	denotes	specific
T5267	15519-15527	NN	denotes	antibody
T5263	15528-15536	NN	denotes	staining
T5268	15536-15538	,	denotes	,
T5269	15538-15541	CC	denotes	but
T5270	15542-15545	DT	denotes	the
T5271	15546-15559	NN	denotes	detectability
T5273	15560-15562	IN	denotes	of
T5274	15563-15568	JJ	denotes	other
T5275	15569-15577	NNS	denotes	antigens
T5276	15578-15583	MD	denotes	might
T5277	15584-15586	VB	denotes	be
T5272	15587-15596	VBN	denotes	decreased
T5278	15596-15597	.	denotes	.
T5279	15597-15676	sentence	denotes	Moreover, the optimal pre-treatment has to be individualised for each antigen.
T5280	15598-15606	RB	denotes	Moreover
T5282	15606-15608	,	denotes	,
T5283	15608-15611	DT	denotes	the
T5285	15612-15619	JJ	denotes	optimal
T5284	15620-15633	NN	denotes	pre-treatment
T5281	15634-15637	VBZ	denotes	has
T5286	15638-15640	TO	denotes	to
T5288	15641-15643	VB	denotes	be
T5287	15644-15658	VBN	denotes	individualised
T5289	15659-15662	IN	denotes	for
T5290	15663-15667	DT	denotes	each
T5291	15668-15675	NN	denotes	antigen
T5292	15675-15676	.	denotes	.
T5293	15676-15910	sentence	denotes	To verify the presence of endogenous Annexin A7 in nuclei, we used a biochemical approach and purified nuclei from neuro-2a, PC-12, and C6 cells and treated them with a hypotonic extraction buffer to obtain the nucleoplasm (Fig. 7A).
T5294	15677-15679	TO	denotes	To
T5295	15680-15686	VB	denotes	verify
T5297	15687-15690	DT	denotes	the
T5298	15691-15699	NN	denotes	presence
T5299	15700-15702	IN	denotes	of
T5300	15703-15713	JJ	denotes	endogenous
T5302	15714-15721	NN	denotes	Annexin
T5301	15722-15724	NN	denotes	A7
T5303	15725-15727	IN	denotes	in
T5304	15728-15734	NNS	denotes	nuclei
T5305	15734-15736	,	denotes	,
T5306	15736-15738	PRP	denotes	we
T5296	15739-15743	VBD	denotes	used
T5307	15744-15745	DT	denotes	a
T5309	15746-15757	JJ	denotes	biochemical
T5308	15758-15766	NN	denotes	approach
T5310	15767-15770	CC	denotes	and
T5311	15771-15779	VBN	denotes	purified
T5312	15780-15786	NNS	denotes	nuclei
T5313	15787-15791	IN	denotes	from
T5314	15792-15797	NN	denotes	neuro
T5316	15797-15798	HYPH	denotes	-
T5317	15798-15800	CD	denotes	2a
T5318	15800-15802	,	denotes	,
T5319	15802-15804	NN	denotes	PC
T5320	15804-15805	HYPH	denotes	-
T5321	15805-15807	CD	denotes	12
T5322	15807-15809	,	denotes	,
T5323	15809-15812	CC	denotes	and
T5324	15813-15815	NN	denotes	C6
T5315	15816-15821	NNS	denotes	cells
T5325	15822-15825	CC	denotes	and
T5326	15826-15833	VBN	denotes	treated
T5327	15834-15838	PRP	denotes	them
T5328	15839-15843	IN	denotes	with
T5329	15844-15845	DT	denotes	a
T5331	15846-15855	JJ	denotes	hypotonic
T5332	15856-15866	NN	denotes	extraction
T5330	15867-15873	NN	denotes	buffer
T5333	15874-15876	TO	denotes	to
T5334	15877-15883	VB	denotes	obtain
T5335	15884-15887	DT	denotes	the
T5336	15888-15899	NN	denotes	nucleoplasm
T5337	15900-15901	-LRB-	denotes	(
T5339	15901-15905	NN	denotes	Fig.
T5338	15906-15908	NN	denotes	7A
T5340	15908-15909	-RRB-	denotes	)
T5341	15909-15910	.	denotes	.
T5342	15910-16058	sentence	denotes	The nucleoplasm and the remaining nuclei, from which the nucleoplasm has been extracted partially, were subjected to SDS-PAGE and Western blotting.
T5343	15911-15914	DT	denotes	The
T5344	15915-15926	NN	denotes	nucleoplasm
T5346	15927-15930	CC	denotes	and
T5347	15931-15934	DT	denotes	the
T5349	15935-15944	VBG	denotes	remaining
T5348	15945-15951	NNS	denotes	nuclei
T5350	15951-15953	,	denotes	,
T5351	15953-15957	IN	denotes	from
T5353	15958-15963	WDT	denotes	which
T5354	15964-15967	DT	denotes	the
T5355	15968-15979	NN	denotes	nucleoplasm
T5356	15980-15983	VBZ	denotes	has
T5357	15984-15988	VBN	denotes	been
T5352	15989-15998	VBN	denotes	extracted
T5358	15999-16008	RB	denotes	partially
T5359	16008-16010	,	denotes	,
T5360	16010-16014	VBD	denotes	were
T5345	16015-16024	VBN	denotes	subjected
T5361	16025-16027	IN	denotes	to
T5362	16028-16031	NN	denotes	SDS
T5364	16031-16032	HYPH	denotes	-
T5363	16032-16036	NN	denotes	PAGE
T5365	16037-16040	CC	denotes	and
T5366	16041-16048	NNP	denotes	Western
T5367	16049-16057	NN	denotes	blotting
T5368	16057-16058	.	denotes	.
T5369	16058-16296	sentence	denotes	Western blots probed with mAb 203–217 showed Annexin A7 in the nucleoplasm of neuronal (neuro-2a, PC-12) and astroglial (C6) cell lines (Fig. 7A), however, the large isoform could only be clearly extracted with nucleoplasm from C6 cells.
T5370	16059-16066	NNP	denotes	Western
T5371	16067-16072	NNS	denotes	blots
T5373	16073-16079	VBN	denotes	probed
T5374	16080-16084	IN	denotes	with
T5375	16085-16088	NN	denotes	mAb
T5376	16089-16092	CD	denotes	203
T5377	16092-16093	SYM	denotes	–
T5378	16093-16096	CD	denotes	217
T5372	16097-16103	VBD	denotes	showed
T5380	16104-16111	NN	denotes	Annexin
T5381	16112-16114	NN	denotes	A7
T5382	16115-16117	IN	denotes	in
T5383	16118-16121	DT	denotes	the
T5384	16122-16133	NN	denotes	nucleoplasm
T5385	16134-16136	IN	denotes	of
T5386	16137-16145	JJ	denotes	neuronal
T5388	16146-16147	-LRB-	denotes	(
T5390	16147-16152	NN	denotes	neuro
T5391	16152-16153	HYPH	denotes	-
T5389	16153-16155	NN	denotes	2a
T5392	16155-16157	,	denotes	,
T5393	16157-16159	NN	denotes	PC
T5394	16159-16160	HYPH	denotes	-
T5395	16160-16162	CD	denotes	12
T5396	16162-16163	-RRB-	denotes	)
T5397	16164-16167	CC	denotes	and
T5398	16168-16178	JJ	denotes	astroglial
T5399	16179-16180	-LRB-	denotes	(
T5400	16180-16182	NN	denotes	C6
T5401	16182-16183	-RRB-	denotes	)
T5402	16184-16188	NN	denotes	cell
T5387	16189-16194	NNS	denotes	lines
T5403	16195-16196	-LRB-	denotes	(
T5405	16196-16200	NN	denotes	Fig.
T5404	16201-16203	NN	denotes	7A
T5406	16203-16204	-RRB-	denotes	)
T5407	16204-16206	,	denotes	,
T5408	16206-16213	RB	denotes	however
T5409	16213-16215	,	denotes	,
T5410	16215-16218	DT	denotes	the
T5412	16219-16224	JJ	denotes	large
T5411	16225-16232	NN	denotes	isoform
T5413	16233-16238	MD	denotes	could
T5414	16239-16243	RB	denotes	only
T5415	16244-16246	VB	denotes	be
T5416	16247-16254	RB	denotes	clearly
T5379	16255-16264	VBN	denotes	extracted
T5417	16265-16269	IN	denotes	with
T5418	16270-16281	NN	denotes	nucleoplasm
T5419	16282-16286	IN	denotes	from
T5420	16287-16289	NN	denotes	C6
T5421	16290-16295	NNS	denotes	cells
T5422	16295-16296	.	denotes	.
T5423	16296-16580	sentence	denotes	For control, antibodies against LAP2α, Emerin and tubulin were used to verify that the nucleoplasm (marker:LAP2α, which is anon-membrane-bound isoform of LAP2) was successfully extracted and also not contaminated by nuclear membranes (marker:Emerin) or by cytoplasm (marker:tubulin).
T5424	16297-16300	IN	denotes	For
T5426	16301-16308	NN	denotes	control
T5427	16308-16310	,	denotes	,
T5428	16310-16320	NNS	denotes	antibodies
T5429	16321-16328	IN	denotes	against
T5430	16329-16334	NN	denotes	LAP2α
T5431	16334-16336	,	denotes	,
T5432	16336-16342	NN	denotes	Emerin
T5433	16343-16346	CC	denotes	and
T5434	16347-16354	NN	denotes	tubulin
T5435	16355-16359	VBD	denotes	were
T5425	16360-16364	VBN	denotes	used
T5436	16365-16367	TO	denotes	to
T5437	16368-16374	VB	denotes	verify
T5438	16375-16379	IN	denotes	that
T5440	16380-16383	DT	denotes	the
T5441	16384-16395	NN	denotes	nucleoplasm
T5442	16396-16397	-LRB-	denotes	(
T5444	16397-16403	NN	denotes	marker
T5445	16403-16404	:	denotes	:
T5443	16404-16409	NN	denotes	LAP2α
T5446	16409-16411	,	denotes	,
T5447	16411-16416	WDT	denotes	which
T5448	16417-16419	VBZ	denotes	is
T5449	16420-16424	NN	denotes	anon
T5451	16424-16425	HYPH	denotes	-
T5450	16425-16433	NN	denotes	membrane
T5453	16433-16434	HYPH	denotes	-
T5452	16434-16439	VBN	denotes	bound
T5454	16440-16447	NN	denotes	isoform
T5455	16448-16450	IN	denotes	of
T5456	16451-16455	NN	denotes	LAP2
T5457	16455-16456	-RRB-	denotes	)
T5458	16457-16460	VBD	denotes	was
T5459	16461-16473	RB	denotes	successfully
T5439	16474-16483	VBN	denotes	extracted
T5460	16484-16487	CC	denotes	and
T5461	16488-16492	RB	denotes	also
T5462	16493-16496	RB	denotes	not
T5463	16497-16509	VBN	denotes	contaminated
T5464	16510-16512	IN	denotes	by
T5465	16513-16520	JJ	denotes	nuclear
T5466	16521-16530	NNS	denotes	membranes
T5467	16531-16532	-LRB-	denotes	(
T5469	16532-16538	NN	denotes	marker
T5470	16538-16539	:	denotes	:
T5468	16539-16545	NN	denotes	Emerin
T5471	16545-16546	-RRB-	denotes	)
T5472	16547-16549	CC	denotes	or
T5473	16550-16552	IN	denotes	by
T5474	16553-16562	NN	denotes	cytoplasm
T5475	16563-16564	-LRB-	denotes	(
T5477	16564-16570	NN	denotes	marker
T5478	16570-16571	:	denotes	:
T5476	16571-16578	NN	denotes	tubulin
T5479	16578-16579	-RRB-	denotes	)
T5480	16579-16580	.	denotes	.
T5481	16580-16730	sentence	denotes	Likewise, in these neuronal and astroglial cell lines Annexin A7 had been observed in the nucleus by immunofluorescence (Fig. 7B and data not shown).
T5482	16581-16589	RB	denotes	Likewise
T5484	16589-16591	,	denotes	,
T5485	16591-16593	IN	denotes	in
T5486	16594-16599	DT	denotes	these
T5488	16600-16608	JJ	denotes	neuronal
T5489	16609-16612	CC	denotes	and
T5490	16613-16623	JJ	denotes	astroglial
T5491	16624-16628	NN	denotes	cell
T5487	16629-16634	NNS	denotes	lines
T5492	16635-16642	NN	denotes	Annexin
T5493	16643-16645	NN	denotes	A7
T5494	16646-16649	VBD	denotes	had
T5495	16650-16654	VBN	denotes	been
T5483	16655-16663	VBN	denotes	observed
T5496	16664-16666	IN	denotes	in
T5497	16667-16670	DT	denotes	the
T5498	16671-16678	NN	denotes	nucleus
T5499	16679-16681	IN	denotes	by
T5500	16682-16700	NN	denotes	immunofluorescence
T5501	16701-16702	-LRB-	denotes	(
T5503	16702-16706	NN	denotes	Fig.
T5502	16707-16709	NN	denotes	7B
T5504	16710-16713	CC	denotes	and
T5505	16714-16718	NNS	denotes	data
T5507	16719-16722	RB	denotes	not
T5506	16723-16728	VBN	denotes	shown
T5508	16728-16729	-RRB-	denotes	)
T5509	16729-16730	.	denotes	.
T5510	16730-16907	sentence	denotes	We noticed no difference between PC-12 and neuro-2a cells, however, as for the brain sections, we found an increase of the Annexin A7 staining after pre-treatment with trypsin.
T5511	16731-16733	PRP	denotes	We
T5512	16734-16741	VBD	denotes	noticed
T5514	16742-16744	DT	denotes	no
T5515	16745-16755	NN	denotes	difference
T5516	16756-16763	IN	denotes	between
T5517	16764-16766	NN	denotes	PC
T5519	16766-16767	HYPH	denotes	-
T5520	16767-16769	CD	denotes	12
T5521	16770-16773	CC	denotes	and
T5522	16774-16779	NN	denotes	neuro
T5524	16779-16780	HYPH	denotes	-
T5523	16780-16782	NN	denotes	2a
T5518	16783-16788	NNS	denotes	cells
T5525	16788-16790	,	denotes	,
T5526	16790-16797	RB	denotes	however
T5527	16797-16799	,	denotes	,
T5528	16799-16801	IN	denotes	as
T5529	16802-16805	IN	denotes	for
T5530	16806-16809	DT	denotes	the
T5532	16810-16815	NN	denotes	brain
T5531	16816-16824	NNS	denotes	sections
T5533	16824-16826	,	denotes	,
T5534	16826-16828	PRP	denotes	we
T5513	16829-16834	VBD	denotes	found
T5535	16835-16837	DT	denotes	an
T5536	16838-16846	NN	denotes	increase
T5537	16847-16849	IN	denotes	of
T5538	16850-16853	DT	denotes	the
T5540	16854-16861	NN	denotes	Annexin
T5541	16862-16864	NN	denotes	A7
T5539	16865-16873	NN	denotes	staining
T5542	16874-16879	IN	denotes	after
T5543	16880-16893	NN	denotes	pre-treatment
T5544	16894-16898	IN	denotes	with
T5545	16899-16906	NN	denotes	trypsin
T5546	16906-16907	.	denotes	.
T14995	16918-16925	JJ	denotes	Nuclear
T14996	16926-16938	NN	denotes	localization
T14997	16939-16941	IN	denotes	of
T14998	16942-16949	NN	denotes	Annexin
T14999	16950-16952	NN	denotes	A7
T15000	16952-16953	.	denotes	.
T15001	16953-17078	sentence	denotes	(A) Extraction of Annexin A7 from nuclei of neuronal (PC-12, N2A) and an astroglial (C6) cell line using a hypotonic buffer.
T15002	16954-16955	-LRB-	denotes	(
T15003	16955-16956	LS	denotes	A
T15005	16956-16957	-RRB-	denotes	)
T15004	16958-16968	NN	denotes	Extraction
T15006	16969-16971	IN	denotes	of
T15007	16972-16979	NN	denotes	Annexin
T15008	16980-16982	NN	denotes	A7
T15009	16983-16987	IN	denotes	from
T15010	16988-16994	NNS	denotes	nuclei
T15011	16995-16997	IN	denotes	of
T15012	16998-17006	JJ	denotes	neuronal
T15013	17007-17008	-LRB-	denotes	(
T15014	17008-17010	NN	denotes	PC
T15015	17010-17011	HYPH	denotes	-
T15016	17011-17013	CD	denotes	12
T15017	17013-17015	,	denotes	,
T15018	17015-17018	NN	denotes	N2A
T15019	17018-17019	-RRB-	denotes	)
T15020	17020-17023	CC	denotes	and
T15021	17024-17026	DT	denotes	an
T15022	17027-17037	JJ	denotes	astroglial
T15024	17038-17039	-LRB-	denotes	(
T15025	17039-17041	NN	denotes	C6
T15026	17041-17042	-RRB-	denotes	)
T15027	17043-17047	NN	denotes	cell
T15023	17048-17052	NN	denotes	line
T15028	17053-17058	VBG	denotes	using
T15029	17059-17060	DT	denotes	a
T15031	17061-17070	JJ	denotes	hypotonic
T15030	17071-17077	NN	denotes	buffer
T15032	17077-17078	.	denotes	.
T15033	17078-17226	sentence	denotes	Samples of total cells (c), and corresponding amounts of nuclear membranes (m) and nucleoplasm (p) were subjected to SDS-PAGE and Western blotting.
T15034	17079-17086	NNS	denotes	Samples
T15036	17087-17089	IN	denotes	of
T15037	17090-17095	JJ	denotes	total
T15038	17096-17101	NNS	denotes	cells
T15039	17102-17103	-LRB-	denotes	(
T15040	17103-17104	NN	denotes	c
T15041	17104-17105	-RRB-	denotes	)
T15042	17105-17107	,	denotes	,
T15043	17107-17110	CC	denotes	and
T15044	17111-17124	JJ	denotes	corresponding
T15045	17125-17132	NNS	denotes	amounts
T15046	17133-17135	IN	denotes	of
T15047	17136-17143	JJ	denotes	nuclear
T15048	17144-17153	NNS	denotes	membranes
T15049	17154-17155	-LRB-	denotes	(
T15050	17155-17156	NN	denotes	m
T15051	17156-17157	-RRB-	denotes	)
T15052	17158-17161	CC	denotes	and
T15053	17162-17173	NN	denotes	nucleoplasm
T15054	17174-17175	-LRB-	denotes	(
T15055	17175-17176	NN	denotes	p
T15056	17176-17177	-RRB-	denotes	)
T15057	17178-17182	VBD	denotes	were
T15035	17183-17192	VBN	denotes	subjected
T15058	17193-17195	IN	denotes	to
T15059	17196-17199	NN	denotes	SDS
T15061	17199-17200	HYPH	denotes	-
T15060	17200-17204	NN	denotes	PAGE
T15062	17205-17208	CC	denotes	and
T15063	17209-17216	NNP	denotes	Western
T15064	17217-17225	NN	denotes	blotting
T15065	17225-17226	.	denotes	.
T15066	17226-17350	sentence	denotes	Annexin A7 (AnxA7) isoforms are extractable from the nucleus of the indicated cells (47 kDa and 51 kDa isoform, arrowhead).
T15067	17227-17234	NN	denotes	Annexin
T15068	17235-17237	NN	denotes	A7
T15070	17238-17239	-LRB-	denotes	(
T15071	17239-17244	NN	denotes	AnxA7
T15072	17244-17245	-RRB-	denotes	)
T15069	17246-17254	NNS	denotes	isoforms
T15073	17255-17258	VBP	denotes	are
T15074	17259-17270	JJ	denotes	extractable
T15075	17271-17275	IN	denotes	from
T15076	17276-17279	DT	denotes	the
T15077	17280-17287	NN	denotes	nucleus
T15078	17288-17290	IN	denotes	of
T15079	17291-17294	DT	denotes	the
T15081	17295-17304	VBN	denotes	indicated
T15080	17305-17310	NNS	denotes	cells
T15082	17311-17312	-LRB-	denotes	(
T15084	17312-17314	CD	denotes	47
T15085	17315-17318	NN	denotes	kDa
T15087	17319-17322	CC	denotes	and
T15088	17323-17325	CD	denotes	51
T15089	17326-17329	NN	denotes	kDa
T15086	17330-17337	NN	denotes	isoform
T15090	17337-17339	,	denotes	,
T15083	17339-17348	NN	denotes	arrowhead
T15091	17348-17349	-RRB-	denotes	)
T15092	17349-17350	.	denotes	.
T15093	17350-17582	sentence	denotes	For control, immunoblotting of LAP2α (75 kDa; non-membrane-bound isoform), Emerin (EM, 34 kDa), and tubulin (TB, 55 kDa) which are specific for nucleoplasm (LAP2α), nuclear membranes (Emerin), and the cytoplasm (tubulin) are shown.
T15094	17351-17354	IN	denotes	For
T15096	17355-17362	NN	denotes	control
T15097	17362-17364	,	denotes	,
T15098	17364-17378	NN	denotes	immunoblotting
T15099	17379-17381	IN	denotes	of
T15100	17382-17387	NN	denotes	LAP2α
T15101	17388-17389	-LRB-	denotes	(
T15103	17389-17391	CD	denotes	75
T15104	17392-17395	NN	denotes	kDa
T15105	17395-17396	:	denotes	;
T15106	17397-17409	JJ	denotes	non-membrane
T15108	17409-17410	HYPH	denotes	-
T15107	17410-17415	VBN	denotes	bound
T15102	17416-17423	NN	denotes	isoform
T15109	17423-17424	-RRB-	denotes	)
T15110	17424-17426	,	denotes	,
T15111	17426-17432	NN	denotes	Emerin
T15112	17433-17434	-LRB-	denotes	(
T15113	17434-17436	NN	denotes	EM
T15114	17436-17438	,	denotes	,
T15116	17438-17440	CD	denotes	34
T15115	17441-17444	NN	denotes	kDa
T15117	17444-17445	-RRB-	denotes	)
T15118	17445-17447	,	denotes	,
T15119	17447-17450	CC	denotes	and
T15120	17451-17458	NN	denotes	tubulin
T15121	17459-17460	-LRB-	denotes	(
T15122	17460-17462	NN	denotes	TB
T15123	17462-17464	,	denotes	,
T15125	17464-17466	CD	denotes	55
T15124	17467-17470	NN	denotes	kDa
T15126	17470-17471	-RRB-	denotes	)
T15127	17472-17477	WDT	denotes	which
T15128	17478-17481	VBP	denotes	are
T15129	17482-17490	JJ	denotes	specific
T15130	17491-17494	IN	denotes	for
T15131	17495-17506	NN	denotes	nucleoplasm
T15132	17507-17508	-LRB-	denotes	(
T15133	17508-17513	NN	denotes	LAP2α
T15134	17513-17514	-RRB-	denotes	)
T15135	17514-17516	,	denotes	,
T15136	17516-17523	JJ	denotes	nuclear
T15137	17524-17533	NNS	denotes	membranes
T15138	17534-17535	-LRB-	denotes	(
T15139	17535-17541	NN	denotes	Emerin
T15140	17541-17542	-RRB-	denotes	)
T15141	17542-17544	,	denotes	,
T15142	17544-17547	CC	denotes	and
T15143	17548-17551	DT	denotes	the
T15144	17552-17561	NN	denotes	cytoplasm
T15145	17562-17563	-LRB-	denotes	(
T15146	17563-17570	NN	denotes	tubulin
T15147	17570-17571	-RRB-	denotes	)
T15148	17572-17575	VBP	denotes	are
T15095	17576-17581	VBN	denotes	shown
T15149	17581-17582	.	denotes	.
T15150	17582-17639	sentence	denotes	Note that the nucleoplasm is only partially extractable.
T15151	17583-17587	VB	denotes	Note
T15152	17588-17592	IN	denotes	that
T15154	17593-17596	DT	denotes	the
T15155	17597-17608	NN	denotes	nucleoplasm
T15153	17609-17611	VBZ	denotes	is
T15156	17612-17616	RB	denotes	only
T15157	17617-17626	RB	denotes	partially
T15158	17627-17638	JJ	denotes	extractable
T15159	17638-17639	.	denotes	.
T15160	17639-17694	sentence	denotes	(B) Immunofluorescence images of the PC-12 cells used.
T15161	17640-17641	-LRB-	denotes	(
T15162	17641-17642	LS	denotes	B
T15164	17642-17643	-RRB-	denotes	)
T15165	17644-17662	NN	denotes	Immunofluorescence
T15163	17663-17669	NNS	denotes	images
T15166	17670-17672	IN	denotes	of
T15167	17673-17676	DT	denotes	the
T15169	17677-17679	NN	denotes	PC
T15170	17679-17680	HYPH	denotes	-
T15171	17680-17682	CD	denotes	12
T15168	17683-17688	NNS	denotes	cells
T15172	17689-17693	VBN	denotes	used
T15173	17693-17694	.	denotes	.
T15174	17694-17880	sentence	denotes	Cells were fixed with paraformaldehyde, permeabilized with Triton X-100, pretreated with trypsin as indicated, and stained with Annexin A7 specific mAb 203–217, DAPI in blue, bar 10 μm.
T15175	17695-17700	NNS	denotes	Cells
T15177	17701-17705	VBD	denotes	were
T15176	17706-17711	VBN	denotes	fixed
T15178	17712-17716	IN	denotes	with
T15179	17717-17733	NN	denotes	paraformaldehyde
T15180	17733-17735	,	denotes	,
T15181	17735-17748	VBN	denotes	permeabilized
T15182	17749-17753	IN	denotes	with
T15183	17754-17760	NN	denotes	Triton
T15184	17761-17762	NN	denotes	X
T15185	17762-17763	HYPH	denotes	-
T15186	17763-17766	CD	denotes	100
T15187	17766-17768	,	denotes	,
T15188	17768-17778	VBN	denotes	pretreated
T15189	17779-17783	IN	denotes	with
T15190	17784-17791	NN	denotes	trypsin
T15191	17792-17794	IN	denotes	as
T15192	17795-17804	VBN	denotes	indicated
T15193	17804-17806	,	denotes	,
T15194	17806-17809	CC	denotes	and
T15195	17810-17817	VBN	denotes	stained
T15196	17818-17822	IN	denotes	with
T15197	17823-17830	NN	denotes	Annexin
T15198	17831-17833	NN	denotes	A7
T15199	17834-17842	JJ	denotes	specific
T15201	17843-17846	NN	denotes	mAb
T15200	17847-17850	CD	denotes	203
T15202	17850-17851	SYM	denotes	–
T15203	17851-17854	CD	denotes	217
T15204	17854-17856	,	denotes	,
T15206	17856-17860	NN	denotes	DAPI
T15207	17861-17863	IN	denotes	in
T15208	17864-17868	JJ	denotes	blue
T15209	17868-17870	,	denotes	,
T15210	17870-17873	NN	denotes	bar
T15211	17874-17876	CD	denotes	10
T15205	17877-17879	NN	denotes	μm
T15212	17879-17880	.	denotes	.
R1000	T2533	T2532	amod	weaker,expression
R1001	T2534	T2535	compound	Annexin,A7
R1002	T2535	T2532	compound	A7,expression
R1003	T2536	T2532	prep	in,expression
R1004	T2537	T2538	det	the,ectoderm
R1005	T2538	T2536	pobj	ectoderm,in
R1006	T2539	T2540	punct	(,2A
R1007	T2540	T2501	parataxis	2A,observed
R1008	T2541	T2540	compound	Fig.,2A
R1009	T2542	T2543	punct	–,C
R1010	T2543	T2540	prep	C,2A
R1011	T2544	T2540	punct	),2A
R1012	T2545	T2501	punct	.,observed
R1013	T2547	T2548	prep	At,detected
R1014	T2549	T2547	pobj	E8,At
R1015	T2550	T2551	compound	Annexin,A7
R1016	T2551	T2548	nsubjpass	A7,detected
R1017	T2552	T2548	aux	can,detected
R1018	T2553	T2548	auxpass	be,detected
R1019	T2554	T2548	prep	in,detected
R1020	T2555	T2556	advmod	almost,tissues
R1021	T2556	T2554	pobj	tissues,in
R1022	T2557	T2556	det	all,tissues
R1023	T2558	T2556	prep	of,tissues
R1024	T2559	T2560	det	the,embryo
R1025	T2560	T2558	pobj	embryo,of
R1026	T2561	T2562	punct	(,shown
R1027	T2562	T2548	parataxis	shown,detected
R1028	T2563	T2562	nsubj	data,shown
R1029	T2564	T2562	neg	not,shown
R1030	T2565	T2562	punct	),shown
R1031	T2566	T2548	punct	.,detected
R1032	T2568	T2569	det	A,correlation
R1033	T2569	T2570	nsubj	correlation,is
R1034	T2571	T2569	prep	between,correlation
R1035	T2572	T2573	det	the,presence
R1036	T2573	T2571	pobj	presence,between
R1037	T2574	T2573	prep	of,presence
R1038	T2575	T2576	compound	Annexin,A7
R1039	T2576	T2574	pobj	A7,of
R1040	T2577	T2573	cc	and,presence
R1041	T2578	T2579	det	the,origin
R1042	T2579	T2573	conj	origin,presence
R1043	T2580	T2579	prep	of,origin
R1044	T2581	T2582	det	a,type
R1045	T2582	T2580	pobj	type,of
R1046	T2583	T2582	compound	cell,type
R1047	T2584	T2582	prep	from,type
R1048	T2585	T2586	quantmod	one,three
R1049	T2586	T2589	nummod	three,layers
R1050	T2587	T2586	quantmod	of,three
R1051	T2588	T2586	quantmod	the,three
R1052	T2589	T2584	pobj	layers,from
R1053	T2590	T2589	compound	germ,layers
R1054	T2591	T2570	neg	not,is
R1055	T2592	T2570	acomp	apparent,is
R1056	T2593	T2570	punct	.,is
R1057	T2595	T2596	nsubj	We,noted
R1058	T2597	T2596	advmod	specifically,noted
R1059	T2598	T2599	det	the,localization
R1060	T2599	T2596	dobj	localization,noted
R1061	T2600	T2599	prep	of,localization
R1062	T2601	T2602	det	the,protein
R1063	T2602	T2600	pobj	protein,of
R1064	T2603	T2596	prep	in,noted
R1065	T2604	T2605	det	the,neuroepithelium
R1066	T2605	T2603	pobj	neuroepithelium,in
R1067	T2606	T2605	prep	of,neuroepithelium
R1068	T2607	T2608	det	the,fold
R1069	T2608	T2606	pobj	fold,of
R1070	T2609	T2608	amod	neural,fold
R1071	T2610	T2608	cc	and,fold
R1072	T2611	T2608	conj	tube,fold
R1073	T2612	T2613	punct	(,E13
R1074	T2613	T2596	parataxis	E13,noted
R1075	T2614	T2615	compound	Fig.,2D
R1076	T2615	T2616	dep	2D,E8
R1077	T2616	T2613	dep	E8,E13
R1078	T2617	T2618	punct	–,F
R1079	T2618	T2615	prep	F,2D
R1080	T2619	T2616	punct	", ",E8
R1081	T2620	T2616	amod	proximal,E8
R1082	T2621	T2616	punct	", ",E8
R1083	T2622	T2613	punct	;,E13
R1084	T2623	T2624	compound	Fig.,2G
R1085	T2624	T2613	dep	2G,E13
R1086	T2625	T2626	punct	–,I
R1087	T2626	T2624	prep	I,2G
R1088	T2627	T2613	punct	", ",E13
R1089	T2628	T2613	amod	distal,E13
R1090	T2629	T2613	punct	", ",E13
R1091	T2630	T2613	punct	),E13
R1092	T2631	T2596	punct	.,noted
R1093	T2633	T2634	prep	At,was
R1094	T2635	T2636	det	the,level
R1095	T2636	T2633	pobj	level,At
R1096	T2637	T2636	amod	subcellular,level
R1097	T2638	T2634	punct	", ",was
R1098	T2639	T2640	compound	Annexin,A7
R1099	T2640	T2634	nsubj	A7,was
R1100	T2641	T2634	acomp	present,was
R1101	T2642	T2634	prep	in,was
R1102	T2643	T2644	compound	punctate,structures
R1103	T2644	T2642	pobj	structures,in
R1104	T2645	T2646	advmod	mainly,in
R1105	T2646	T2634	prep	in,was
R1106	T2647	T2648	det	the,cytosol
R1107	T2648	T2646	pobj	cytosol,in
R1108	T2649	T2648	prep	of,cytosol
R1109	T2650	T2651	det	all,cells
R1110	T2651	T2649	pobj	cells,of
R1111	T2652	T2651	amod	examined,cells
R1112	T2653	T2654	punct	(,F
R1113	T2654	T2634	parataxis	F,was
R1114	T2655	T2654	nmod	Fig.,F
R1115	T2656	T2654	nmod	2E,F
R1116	T2657	T2654	punct	",",F
R1117	T2658	T2654	punct	),F
R1118	T2659	T2634	punct	.,was
R1119	T2661	T2662	det	This,distribution
R1120	T2662	T2663	nsubjpass	distribution,observed
R1121	T2664	T2663	auxpass	was,observed
R1122	T2665	T2663	advmod	also,observed
R1123	T2666	T2663	prep	during,observed
R1124	T2667	T2668	amod	subsequent,development
R1125	T2668	T2666	pobj	development,during
R1126	T2669	T2663	punct	.,observed
R1127	T2973	T2974	compound	Annexin,A7
R1128	T2974	T2975	nsubj	A7,changes
R1129	T2976	T2977	poss	its,localization
R1130	T2977	T2975	dobj	localization,changes
R1131	T2978	T2977	amod	subcellular,localization
R1132	T2979	T2975	prep	in,changes
R1133	T2980	T2981	det	the,brain
R1134	T2981	T2979	pobj	brain,in
R1135	T2982	T2981	amod	embryonic,brain
R1136	T2983	T2975	prep	at,changes
R1137	T2984	T2983	pobj	E16,at
R1138	T2986	T2987	advmod	Next,analysed
R1139	T2988	T2987	nsubj	we,analysed
R1140	T2989	T2990	det	the,distribution
R1141	T2990	T2987	dobj	distribution,analysed
R1142	T2991	T2990	amod	cellular,distribution
R1143	T2992	T2991	cc	and,cellular
R1144	T2993	T2991	conj	subcellular,cellular
R1145	T2994	T2990	prep	of,distribution
R1146	T2995	T2996	compound	Annexin,A7
R1147	T2996	T2994	pobj	A7,of
R1148	T2997	T2990	prep	in,distribution
R1149	T2998	T2999	det	the,brain
R1150	T2999	T2997	pobj	brain,in
R1151	T3000	T2999	amod	developing,brain
R1152	T3001	T2999	compound	mouse,brain
R1153	T3002	T3003	punct	(,Fig.
R1154	T3003	T2987	parataxis	Fig.,analysed
R1155	T3004	T3003	nummod	3,Fig.
R1156	T3005	T3003	punct	),Fig.
R1157	T3006	T2987	punct	.,analysed
R1158	T3008	T3009	prep	At,are
R1159	T3010	T3008	pobj	E13,At
R1160	T3011	T3012	punct	–,E16
R1161	T3012	T3010	prep	E16,E13
R1162	T3013	T3009	prep	in,are
R1163	T3014	T3015	amod	transverse,sections
R1164	T3015	T3013	pobj	sections,in
R1165	T3016	T3015	prep	of,sections
R1166	T3017	T3018	det	the,brain
R1167	T3018	T3016	pobj	brain,of
R1168	T3019	T3018	amod	embryonic,brain
R1169	T3020	T3009	punct	", ",are
R1170	T3021	T3009	nsubj	most,are
R1171	T3022	T3021	prep	of,most
R1172	T3023	T3024	det	the,cells
R1173	T3024	T3022	pobj	cells,of
R1174	T3025	T3024	amod	immunoreactive,cells
R1175	T3026	T3009	acomp	present,are
R1176	T3027	T3009	prep	in,are
R1177	T3028	T3029	det	the,zone
R1178	T3029	T3027	pobj	zone,in
R1179	T3030	T3029	amod	ventricular,zone
R1180	T3031	T3029	amod	germinative,zone
R1181	T3032	T3029	acl	surrounding,zone
R1182	T3033	T3034	det	the,ventricle
R1183	T3034	T3032	dobj	ventricle,surrounding
R1184	T3035	T3034	amod	lateral,ventricle
R1185	T3036	T3037	punct	(,E13
R1186	T3037	T3009	parataxis	E13,are
R1187	T3038	T3039	dep	Fig.,E16
R1188	T3039	T3037	dep	E16,E13
R1189	T3040	T3038	nummod	3A,Fig.
R1190	T3041	T3039	punct	", ",E16
R1191	T3042	T3039	compound	overview,E16
R1192	T3043	T3037	punct	;,E13
R1193	T3044	T3037	dep	Fig.,E13
R1194	T3045	T3044	nummod	3H,Fig.
R1195	T3046	T3037	punct	", ",E13
R1196	T3047	T3037	compound	overview,E13
R1197	T3048	T3037	punct	),E13
R1198	T3049	T3009	punct	.,are
R1199	T3051	T3052	det	A,inspection
R1200	T3052	T3054	nsubj	inspection,revealed
R1201	T3053	T3052	amod	closer,inspection
R1202	T3055	T3056	mark	that,localized
R1203	T3056	T3054	ccomp	localized,revealed
R1204	T3057	T3058	compound	Annexin,A7
R1205	T3058	T3056	nsubjpass	A7,localized
R1206	T3059	T3056	auxpass	is,localized
R1207	T3060	T3056	advmod	mainly,localized
R1208	T3061	T3056	prep	in,localized
R1209	T3062	T3063	det	the,cytosol
R1210	T3063	T3061	pobj	cytosol,in
R1211	T3064	T3056	prep	at,localized
R1212	T3065	T3064	pobj	E13,at
R1213	T3066	T3067	punct	(,E
R1214	T3067	T3054	parataxis	E,revealed
R1215	T3068	T3067	nmod	Fig.,E
R1216	T3069	T3067	nummod	3B,E
R1217	T3070	T3067	punct	",",E
R1218	T3071	T3067	punct	),E
R1219	T3072	T3054	punct	.,revealed
R1220	T3074	T3075	prep	During,rounded
R1221	T3076	T3077	amod	further,development
R1222	T3077	T3074	pobj	development,During
R1223	T3078	T3079	det	the,cells
R1224	T3079	T3075	nsubj	cells,rounded
R1225	T3080	T3079	amod	immature,cells
R1226	T3081	T3075	aux	have,rounded
R1227	T3082	T3075	prt	up,rounded
R1228	T3083	T3084	nummod	two,days
R1229	T3084	T3085	npadvmod	days,later
R1230	T3085	T3075	advmod	later,rounded
R1231	T3086	T3075	punct	", ",rounded
R1232	T3087	T3075	cc	but,rounded
R1233	T3088	T3089	nsubj	they,retain
R1234	T3089	T3075	conj	retain,rounded
R1235	T3090	T3091	compound	Annexin,A7
R1236	T3091	T3089	dobj	A7,retain
R1237	T3092	T3089	prep	in,retain
R1238	T3093	T3094	det	the,cytosol
R1239	T3094	T3092	pobj	cytosol,in
R1240	T3095	T3089	prep	at,retain
R1241	T3096	T3095	pobj	E15,at
R1242	T3097	T3098	punct	(,F
R1243	T3098	T3089	parataxis	F,retain
R1244	T3099	T3098	nmod	Fig.,F
R1245	T3100	T3098	nummod	3C,F
R1246	T3101	T3098	punct	",",F
R1247	T3102	T3098	punct	),F
R1248	T3103	T3075	punct	.,rounded
R1249	T3105	T3106	prep	At,observed
R1250	T3107	T3105	pobj	E16,At
R1251	T3108	T3106	nsubj	we,observed
R1252	T3109	T3110	det	the,staining
R1253	T3110	T3106	dobj	staining,observed
R1254	T3111	T3110	amod	first,staining
R1255	T3112	T3110	amod	prominent,staining
R1256	T3113	T3110	amod	nuclear,staining
R1257	T3114	T3110	prep	of,staining
R1258	T3115	T3116	compound	Annexin,A7
R1259	T3116	T3114	pobj	A7,of
R1260	T3117	T3118	punct	(,G
R1261	T3118	T3110	parataxis	G,staining
R1262	T3119	T3118	nmod	Fig.,G
R1263	T3120	T3118	nmod	3D,G
R1264	T3121	T3118	punct	",",G
R1265	T3122	T3118	punct	),G
R1266	T3123	T3106	prep	in,observed
R1267	T3124	T3123	pobj	cells,in
R1268	T3125	T3124	prep	of,cells
R1269	T3126	T3127	det	the,zone
R1270	T3127	T3125	pobj	zone,of
R1271	T3128	T3127	amod	intermediate,zone
R1272	T3129	T3130	punct	(,oval
R1273	T3130	T3127	parataxis	oval,zone
R1274	T3131	T3132	compound	Fig.,3I
R1275	T3132	T3130	dep	3I,oval
R1276	T3133	T3130	punct	", ",oval
R1277	T3134	T3130	punct	),oval
R1278	T3135	T3127	punct	", ",zone
R1279	T3136	T3137	dep	which,contains
R1280	T3137	T3127	relcl	contains,zone
R1281	T3138	T3137	dobj	neurons,contains
R1282	T3139	T3140	advmod	radially,migrating
R1283	T3140	T3138	acl	migrating,neurons
R1284	T3141	T3140	prep	towards,migrating
R1285	T3142	T3143	det	the,cortex
R1286	T3143	T3141	pobj	cortex,towards
R1287	T3144	T3143	amod	growing,cortex
R1288	T3145	T3143	compound	neopallium,cortex
R1289	T3146	T3147	punct	[,14
R1290	T3147	T3143	parataxis	14,cortex
R1291	T3148	T3147	nummod	13,14
R1292	T3149	T3147	punct	",",14
R1293	T3150	T3147	punct	],14
R1294	T3151	T3138	cc	and,neurons
R1295	T3152	T3151	advmod	also,and
R1296	T3153	T3154	amod	glial,cells
R1297	T3154	T3138	conj	cells,neurons
R1298	T3155	T3154	acl	forming,cells
R1299	T3156	T3157	det	the,matter
R1300	T3157	T3155	dobj	matter,forming
R1301	T3158	T3157	amod	white,matter
R1302	T3159	T3157	prep	of,matter
R1303	T3160	T3161	det	the,cortex
R1304	T3161	T3159	pobj	cortex,of
R1305	T3162	T3161	amod	adult,cortex
R1306	T3163	T3106	punct	.,observed
R1307	T3165	T3166	nsubj	Cells,exhibit
R1308	T3167	T3165	acl	located,Cells
R1309	T3168	T3169	advmod	marginally,in
R1310	T3169	T3167	prep	in,located
R1311	T3170	T3171	det	the,cortex
R1312	T3171	T3169	pobj	cortex,in
R1313	T3172	T3171	amod	neopallial,cortex
R1314	T3173	T3166	advmod	also,exhibit
R1315	T3174	T3175	det	a,stain
R1316	T3175	T3166	dobj	stain,exhibit
R1317	T3176	T3175	amod	nuclear,stain
R1318	T3177	T3166	punct	.,exhibit
R1319	T3179	T3180	nsubj	Labelling,is
R1320	T3181	T3179	prep	in,Labelling
R1321	T3182	T3183	det	the,zone
R1322	T3183	T3181	pobj	zone,in
R1323	T3184	T3183	amod	ventricular,zone
R1324	T3185	T3183	amod	germinative,zone
R1325	T3186	T3187	advmod	less,prominent
R1326	T3187	T3180	acomp	prominent,is
R1327	T3188	T3180	cc	and,is
R1328	T3189	T3190	det	the,cytosol
R1329	T3190	T3191	nsubj	cytosol,is
R1330	T3191	T3180	conj	is,is
R1331	T3192	T3193	neg	no,longer
R1332	T3193	T3191	advmod	longer,is
R1333	T3194	T3195	advmod	more,strongly
R1334	T3195	T3196	advmod	strongly,stained
R1335	T3196	T3191	acomp	stained,is
R1336	T3197	T3196	prep	than,stained
R1337	T3198	T3199	det	the,nucleus
R1338	T3199	T3197	pobj	nucleus,than
R1339	T3200	T3191	punct	.,is
R1340	T3569	T3570	amod	Mature,neurons
R1341	T3570	T3571	nsubj	neurons,show
R1342	T3572	T3570	prep	in,neurons
R1343	T3573	T3574	amod	adult,brain
R1344	T3574	T3572	pobj	brain,in
R1345	T3575	T3574	compound	mouse,brain
R1346	T3576	T3577	det	an,staining
R1347	T3577	T3571	dobj	staining,show
R1348	T3578	T3577	amod	intense,staining
R1349	T3579	T3577	amod	nuclear,staining
R1350	T3580	T3577	prep	of,staining
R1351	T3581	T3582	compound	Annexin,A7
R1352	T3582	T3580	pobj	A7,of
R1353	T3584	T3585	preconj	Both,isoforms
R1354	T3585	T3588	nsubjpass	isoforms,found
R1355	T3586	T3587	compound	Annexin,A7
R1356	T3587	T3585	compound	A7,isoforms
R1357	T3589	T3588	auxpass	are,found
R1358	T3590	T3588	prep	in,found
R1359	T3591	T3592	amod	adult,tissue
R1360	T3592	T3590	pobj	tissue,in
R1361	T3593	T3592	compound	brain,tissue
R1362	T3594	T3595	punct	(,1B
R1363	T3595	T3588	parataxis	1B,found
R1364	T3596	T3595	compound	Fig.,1B
R1365	T3597	T3595	punct	),1B
R1366	T3598	T3588	punct	.,found
R1367	T3600	T3601	prep	In,observed
R1368	T3602	T3600	amod	general,In
R1369	T3603	T3601	punct	", ",observed
R1370	T3604	T3601	nsubj	we,observed
R1371	T3605	T3606	nummod	two,patterns
R1372	T3606	T3601	dobj	patterns,observed
R1373	T3607	T3606	amod	characteristic,patterns
R1374	T3608	T3606	compound	staining,patterns
R1375	T3609	T3606	prep	for,patterns
R1376	T3610	T3611	compound	Annexin,A7
R1377	T3611	T3609	pobj	A7,for
R1378	T3612	T3606	punct	", ",patterns
R1379	T3613	T3614	det	a,stain
R1380	T3614	T3606	appos	stain,patterns
R1381	T3615	T3614	amod	prominent,stain
R1382	T3616	T3614	amod	nuclear,stain
R1383	T3617	T3614	prep	in,stain
R1384	T3618	T3617	pobj	neurons,in
R1385	T3619	T3614	punct	(,stain
R1386	T3620	T3614	acl	determined,stain
R1387	T3621	T3620	prep	by,determined
R1388	T3622	T3621	pobj	lack,by
R1389	T3623	T3622	prep	of,lack
R1390	T3624	T3625	nmod	GFAP,morphology
R1391	T3625	T3623	pobj	morphology,of
R1392	T3626	T3625	punct	-,morphology
R1393	T3627	T3625	nmod	immunoreactivity,morphology
R1394	T3628	T3625	punct	", ",morphology
R1395	T3629	T3625	nmod	localization,morphology
R1396	T3630	T3625	punct	", ",morphology
R1397	T3631	T3614	punct	),stain
R1398	T3632	T3614	cc	and,stain
R1399	T3633	T3634	det	a,stain
R1400	T3634	T3614	conj	stain,stain
R1401	T3635	T3634	amod	cytoplasmic,stain
R1402	T3636	T3635	cc	and,cytoplasmic
R1403	T3637	T3635	conj	nuclear,cytoplasmic
R1404	T3638	T3634	prep	in,stain
R1405	T3639	T3638	pobj	astrocytes,in
R1406	T3640	T3641	punct	(,positive
R1407	T3641	T3639	parataxis	positive,astrocytes
R1408	T3642	T3641	npadvmod	GFAP,positive
R1409	T3643	T3641	punct	-,positive
R1410	T3644	T3641	punct	),positive
R1411	T3645	T3601	prep	in,observed
R1412	T3646	T3647	det	all,areas
R1413	T3647	T3645	pobj	areas,in
R1414	T3648	T3647	prep	of,areas
R1415	T3649	T3650	det	the,brain
R1416	T3650	T3648	pobj	brain,of
R1417	T3651	T3650	amod	mature,brain
R1418	T3652	T3650	amod	murine,brain
R1419	T3653	T3601	punct	.,observed
R1420	T3655	T3656	prep	In,enriched
R1421	T3657	T3658	det	the,neocortex
R1422	T3658	T3655	pobj	neocortex,In
R1423	T3659	T3660	punct	(,isocortex
R1424	T3660	T3658	parataxis	isocortex,neocortex
R1425	T3661	T3660	punct	),isocortex
R1426	T3662	T3663	compound	Annexin,A7
R1427	T3663	T3656	nsubjpass	A7,enriched
R1428	T3664	T3656	auxpass	was,enriched
R1429	T3665	T3656	advmod	strongly,enriched
R1430	T3666	T3656	prep	in,enriched
R1431	T3667	T3666	pobj	nuclei,in
R1432	T3668	T3667	prep	of,nuclei
R1433	T3669	T3668	pobj	neurons,of
R1434	T3670	T3669	prep	of,neurons
R1435	T3671	T3672	det	all,laminae
R1436	T3672	T3670	pobj	laminae,of
R1437	T3673	T3672	nummod	six,laminae
R1438	T3674	T3672	amod	cortical,laminae
R1439	T3675	T3676	punct	(,section
R1440	T3676	T3656	parataxis	section,enriched
R1441	T3677	T3678	compound	Fig.,4A
R1442	T3678	T3676	dep	4A,section
R1443	T3679	T3676	punct	", ",section
R1444	T3680	T3676	acl	derived,section
R1445	T3681	T3680	prep	from,derived
R1446	T3682	T3683	compound	cortex,temporalis
R1447	T3683	T3681	pobj	temporalis,from
R1448	T3684	T3676	punct	),section
R1449	T3685	T3656	punct	.,enriched
R1450	T3687	T3688	prep	For,characterized
R1451	T3689	T3687	pobj	control,For
R1452	T3690	T3688	punct	", ",characterized
R1453	T3691	T3688	prep	in,characterized
R1454	T3692	T3693	det	the,brain
R1455	T3693	T3691	pobj	brain,in
R1456	T3694	T3693	amod	adult,brain
R1457	T3695	T3696	nmod	type,astrocytes
R1458	T3696	T3688	nsubjpass	astrocytes,characterized
R1459	T3697	T3695	punct	-,type
R1460	T3698	T3695	nummod	1,type
R1461	T3699	T3688	aux	can,characterized
R1462	T3700	T3688	auxpass	be,characterized
R1463	T3701	T3688	agent	by,characterized
R1464	T3702	T3703	det	a,stain
R1465	T3703	T3701	pobj	stain,by
R1466	T3704	T3703	amod	positive,stain
R1467	T3705	T3703	compound	GFAP,stain
R1468	T3706	T3703	punct	-,stain
R1469	T3707	T3708	punct	(,4B
R1470	T3708	T3688	parataxis	4B,characterized
R1471	T3709	T3708	compound	Fig.,4B
R1472	T3710	T3708	punct	),4B
R1473	T3711	T3688	punct	.,characterized
R1474	T3713	T3714	compound	Fig.,4F
R1475	T3714	T3715	nsubj	4F,demonstrates
R1476	T3716	T3717	det	the,presence
R1477	T3717	T3715	dobj	presence,demonstrates
R1478	T3718	T3717	prep	of,presence
R1479	T3719	T3720	amod	nuclear,A7
R1480	T3720	T3718	pobj	A7,of
R1481	T3721	T3720	compound	Annexin,A7
R1482	T3722	T3715	prep	in,demonstrates
R1483	T3723	T3724	det	a,neuron
R1484	T3724	T3722	pobj	neuron,in
R1485	T3725	T3724	amod	neocortical,neuron
R1486	T3726	T3724	prep	of,neuron
R1487	T3727	T3728	det	the,layer
R1488	T3728	T3726	pobj	layer,of
R1489	T3729	T3728	amod	external,layer
R1490	T3730	T3728	amod	granular,layer
R1491	T3731	T3732	punct	(,layer
R1492	T3732	T3728	parataxis	layer,layer
R1493	T3733	T3732	nummod	II,layer
R1494	T3734	T3732	punct	),layer
R1495	T3735	T3722	cc	and,in
R1496	T3736	T3722	conj	in,in
R1497	T3737	T3738	det	an,cell
R1498	T3738	T3736	pobj	cell,in
R1499	T3739	T3738	amod	astrocytic,cell
R1500	T3740	T3738	prep	of,cell
R1501	T3741	T3742	det	the,layer
R1502	T3742	T3740	pobj	layer,of
R1503	T3743	T3742	amod	neocortical,layer
R1504	T3744	T3742	amod	molecular,layer
R1505	T3745	T3746	punct	(,layer
R1506	T3746	T3742	parataxis	layer,layer
R1507	T3747	T3746	nummod	I,layer
R1508	T3748	T3746	punct	),layer
R1509	T3749	T3750	dep	which,exhibits
R1510	T3750	T3738	relcl	exhibits,cell
R1511	T3751	T3750	advmod	additionally,exhibits
R1512	T3752	T3753	det	an,signal
R1513	T3753	T3750	dobj	signal,exhibits
R1514	T3754	T3753	amod	intense,signal
R1515	T3755	T3750	prep	in,exhibits
R1516	T3756	T3757	det	the,cytoplasm
R1517	T3757	T3755	pobj	cytoplasm,in
R1518	T3758	T3755	cc	and,in
R1519	T3759	T3755	conj	in,in
R1520	T3760	T3761	amod	cellular,branches
R1521	T3761	T3759	pobj	branches,in
R1522	T3762	T3715	punct	.,demonstrates
R1523	T3764	T3765	det	A,type
R1524	T3765	T3768	nsubj	type,showed
R1525	T3766	T3765	amod	further,type
R1526	T3767	T3765	compound	cell,type
R1527	T3769	T3765	prep	of,type
R1528	T3770	T3771	det	the,isocortex
R1529	T3771	T3769	pobj	isocortex,of
R1530	T3772	T3773	det	a,immunoreactivity
R1531	T3773	T3768	dobj	immunoreactivity,showed
R1532	T3774	T3773	amod	strong,immunoreactivity
R1533	T3775	T3768	prep	in,showed
R1534	T3776	T3777	preconj	both,nucleus
R1535	T3777	T3775	pobj	nucleus,in
R1536	T3778	T3777	punct	", ",nucleus
R1537	T3779	T3777	cc	and,nucleus
R1538	T3780	T3777	conj	cytosol,nucleus
R1539	T3781	T3768	punct	", ",showed
R1540	T3782	T3768	cc	and,showed
R1541	T3783	T3784	auxpass	was,identified
R1542	T3784	T3768	conj	identified,showed
R1543	T3785	T3784	prep	as,identified
R1544	T3786	T3787	amod	pyramidal,neuron
R1545	T3787	T3785	pobj	neuron,as
R1546	T3788	T3784	prep	based,identified
R1547	T3789	T3788	prep	on,based
R1548	T3790	T3789	pobj	morphology,on
R1549	T3791	T3790	cc	and,morphology
R1550	T3792	T3790	conj	absence,morphology
R1551	T3793	T3792	prep	of,absence
R1552	T3794	T3795	compound	GFAP,immunoreactivity
R1553	T3795	T3793	pobj	immunoreactivity,of
R1554	T3796	T3768	punct	.,showed
R1555	T3798	T3799	advmod	However,is
R1556	T3800	T3799	punct	", ",is
R1557	T3801	T3802	compound	Annexin,A7
R1558	T3802	T3799	nsubj	A7,is
R1559	T3803	T3799	advmod	apparently,is
R1560	T3804	T3805	advmod	more,enriched
R1561	T3805	T3799	acomp	enriched,is
R1562	T3806	T3799	prep	in,is
R1563	T3807	T3808	det	the,nucleus
R1564	T3808	T3806	pobj	nucleus,in
R1565	T3809	T3810	punct	(,4E
R1566	T3810	T3799	parataxis	4E,is
R1567	T3811	T3810	compound	Fig.,4E
R1568	T3812	T3810	punct	),4E
R1569	T3813	T3799	punct	.,is
R1570	T3816	T3817	prep	In,detected
R1571	T3818	T3819	det	the,formation
R1572	T3819	T3816	pobj	formation,In
R1573	T3820	T3819	amod	hippocampal,formation
R1574	T3821	T3817	nsubj	we,detected
R1575	T3822	T3823	amod	prominent,immunostaining
R1576	T3823	T3817	dobj	immunostaining,detected
R1577	T3824	T3825	compound	Annexin,A7
R1578	T3825	T3823	compound	A7,immunostaining
R1579	T3826	T3823	prep	in,immunostaining
R1580	T3827	T3828	det	the,pyramidalis
R1581	T3828	T3826	pobj	pyramidalis,in
R1582	T3829	T3828	compound	stratum,pyramidalis
R1583	T3830	T3826	cc	and,in
R1584	T3831	T3826	conj	in,in
R1585	T3832	T3833	det	the,gyrus
R1586	T3833	T3831	pobj	gyrus,in
R1587	T3834	T3833	compound	dentate,gyrus
R1588	T3835	T3817	cc	and,detected
R1589	T3836	T3835	advmod	also,and
R1590	T3837	T3838	det	a,staining
R1591	T3838	T3817	conj	staining,detected
R1592	T3839	T3838	amod	weak,staining
R1593	T3840	T3838	amod	astrocytic,staining
R1594	T3841	T3842	punct	(,4C
R1595	T3842	T3838	parataxis	4C,staining
R1596	T3843	T3842	compound	Fig.,4C
R1597	T3844	T3842	punct	),4C
R1598	T3845	T3817	punct	.,detected
R1599	T3847	T3848	det	The,astrocytes
R1600	T3848	T3849	nsubjpass	astrocytes,localized
R1601	T3850	T3849	auxpass	are,localized
R1602	T3851	T3849	advmod	mainly,localized
R1603	T3852	T3849	prep	in,localized
R1604	T3853	T3854	det	the,area
R1605	T3854	T3852	pobj	area,in
R1606	T3855	T3854	prep	between,area
R1607	T3856	T3855	pobj	cells,between
R1608	T3857	T3856	acl	exhibiting,cells
R1609	T3858	T3859	amod	nuclear,staining
R1610	T3859	T3857	dobj	staining,exhibiting
R1611	T3860	T3861	compound	Annexin,A7
R1612	T3861	T3859	compound	A7,staining
R1613	T3862	T3849	cc	and,localized
R1614	T3863	T3849	conj	show,localized
R1615	T3864	T3865	det	the,protein
R1616	T3865	T3863	dobj	protein,show
R1617	T3866	T3863	advmod	also,show
R1618	T3867	T3863	prep	in,show
R1619	T3868	T3869	det	the,nucleus
R1620	T3869	T3867	pobj	nucleus,in
R1621	T3870	T3869	cc	and,nucleus
R1622	T3871	T3872	det	the,cytoplasm
R1623	T3872	T3869	conj	cytoplasm,nucleus
R1624	T3873	T3874	punct	(,shown
R1625	T3874	T3863	parataxis	shown,show
R1626	T3875	T3874	nsubj	data,shown
R1627	T3876	T3874	neg	not,shown
R1628	T3877	T3874	punct	),shown
R1629	T3878	T3849	punct	.,localized
R1630	T3880	T3881	advmod	When,analyzed
R1631	T3881	T3883	advcl	analyzed,found
R1632	T3882	T3881	nsubj	we,analyzed
R1633	T3884	T3885	det	the,neurons
R1634	T3885	T3881	dobj	neurons,analyzed
R1635	T3886	T3885	amod	pyramidal,neurons
R1636	T3887	T3881	prep	in,analyzed
R1637	T3888	T3889	det	the,hippocampus
R1638	T3889	T3887	pobj	hippocampus,in
R1639	T3890	T3881	prep	at,analyzed
R1640	T3891	T3892	det	a,magnification
R1641	T3892	T3890	pobj	magnification,at
R1642	T3893	T3892	amod	higher,magnification
R1643	T3894	T3883	nsubj	we,found
R1644	T3895	T3896	mark	that,localized
R1645	T3896	T3883	ccomp	localized,found
R1646	T3897	T3898	compound	Annexin,A7
R1647	T3898	T3896	nsubj	A7,localized
R1648	T3899	T3896	advmod	again,localized
R1649	T3900	T3896	advmod	mainly,localized
R1650	T3901	T3896	prep	to,localized
R1651	T3902	T3903	det	the,nuclei
R1652	T3903	T3901	pobj	nuclei,to
R1653	T3904	T3905	punct	(,4G
R1654	T3905	T3883	parataxis	4G,found
R1655	T3906	T3905	compound	Fig.,4G
R1656	T3907	T3905	punct	),4G
R1657	T3908	T3883	punct	.,found
R1658	T3910	T3911	amod	Polyclonal,antibodies
R1659	T3911	T3912	nsubj	antibodies,gave
R1660	T3913	T3914	det	a,pattern
R1661	T3914	T3912	dobj	pattern,gave
R1662	T3915	T3914	amod	similar,pattern
R1663	T3916	T3914	compound	staining,pattern
R1664	T3917	T3918	punct	(,shown
R1665	T3918	T3912	parataxis	shown,gave
R1666	T3919	T3918	nsubj	data,shown
R1667	T3920	T3918	neg	not,shown
R1668	T3921	T3918	punct	),shown
R1669	T3922	T3912	punct	.,gave
R1670	T3924	T3925	det	The,staining
R1671	T3925	T3928	nsubj	staining,was
R1672	T3926	T3925	amod	intense,staining
R1673	T3927	T3925	amod	nuclear,staining
R1674	T3929	T3928	acomp	absent,was
R1675	T3930	T3928	prep	in,was
R1676	T3931	T3930	pobj	controls,in
R1677	T3932	T3933	preconj	either,stained
R1678	T3933	T3931	acl	stained,controls
R1679	T3934	T3935	advmod	only,with
R1680	T3935	T3933	prep	with,stained
R1681	T3936	T3937	det	the,antibody
R1682	T3937	T3935	pobj	antibody,with
R1683	T3938	T3937	amod	secondary,antibody
R1684	T3939	T3940	punct	(,4D
R1685	T3940	T3933	parataxis	4D,stained
R1686	T3941	T3940	compound	Fig.,4D
R1687	T3942	T3940	punct	),4D
R1688	T3943	T3930	cc	or,in
R1689	T3944	T3930	conj	in,in
R1690	T3945	T3946	compound	brain,sections
R1691	T3946	T3944	pobj	sections,in
R1692	T3947	T3946	acl	derived,sections
R1693	T3948	T3947	prep	from,derived
R1694	T3949	T3950	nmod	AnxA7,mice
R1695	T3950	T3948	pobj	mice,from
R1696	T3951	T3949	punct	-,AnxA7
R1697	T3952	T3949	punct	/,AnxA7
R1698	T3953	T3949	punct	-,AnxA7
R1699	T3954	T3950	acl	stained,mice
R1700	T3955	T3954	prep	for,stained
R1701	T3956	T3957	compound	Annexin,A7
R1702	T3957	T3955	pobj	A7,for
R1703	T3958	T3959	punct	(,4H
R1704	T3959	T3946	parataxis	4H,sections
R1705	T3960	T3959	compound	Fig.,4H
R1706	T3961	T3959	punct	),4H
R1707	T3962	T3928	punct	.,was
R1708	T3964	T3965	det	The,staining
R1709	T3965	T3967	nsubj	staining,is
R1710	T3966	T3965	amod	residual,staining
R1711	T3968	T3965	acl	seen,staining
R1712	T3969	T3968	prep	in,seen
R1713	T3970	T3971	det	the,brain
R1714	T3971	T3969	pobj	brain,in
R1715	T3972	T3971	nmod	AnxA7,brain
R1716	T3973	T3972	punct	-,AnxA7
R1717	T3974	T3972	punct	/,AnxA7
R1718	T3975	T3972	punct	-,AnxA7
R1719	T3976	T3967	acomp	unspecific,is
R1720	T3977	T3978	mark	as,is
R1721	T3978	T3967	advcl	is,is
R1722	T3979	T3978	nsubj	it,is
R1723	T3980	T3978	advmod	also,is
R1724	T3981	T3978	acomp	present,is
R1725	T3982	T3978	prep	in,is
R1726	T3983	T3984	amod	corresponding,controls
R1727	T3984	T3982	pobj	controls,in
R1728	T3985	T3984	amod	negative,controls
R1729	T3986	T3984	acl	lacking,controls
R1730	T3987	T3988	det	the,antibody
R1731	T3988	T3986	dobj	antibody,lacking
R1732	T3989	T3988	amod	primary,antibody
R1733	T3990	T3967	punct	.,is
R1734	T3992	T3993	det	The,absence
R1735	T3993	T3994	nsubjpass	absence,verified
R1736	T3995	T3993	prep	of,absence
R1737	T3996	T3997	compound	Annexin,A7
R1738	T3997	T3998	compound	A7,protein
R1739	T3998	T3995	pobj	protein,of
R1740	T3999	T3993	prep	in,absence
R1741	T4000	T3999	pobj	brain,in
R1742	T4001	T4000	cc	and,brain
R1743	T4002	T4003	amod	other,tissues
R1744	T4003	T4000	conj	tissues,brain
R1745	T4004	T4000	prep	of,brain
R1746	T4005	T4006	det	the,mouse
R1747	T4006	T4004	pobj	mouse,of
R1748	T4007	T4006	nmod	AnxA7,mouse
R1749	T4008	T4007	punct	-,AnxA7
R1750	T4009	T4007	punct	/,AnxA7
R1751	T4010	T4007	punct	-,AnxA7
R1752	T4011	T3994	aux	has,verified
R1753	T4012	T3994	auxpass	been,verified
R1754	T4013	T3994	prep	in,verified
R1755	T4014	T4013	punct	[,in
R1756	T4015	T4013	pobj	10,in
R1757	T4016	T3994	punct	],verified
R1758	T4017	T3994	punct	.,verified
R1759	T4019	T4020	prep	In,detected
R1760	T4021	T4022	det	another,study
R1761	T4022	T4019	pobj	study,In
R1762	T4023	T4022	amod	actual,study
R1763	T4024	T4022	prep	of,study
R1764	T4025	T4026	det	the,mouse
R1765	T4026	T4024	pobj	mouse,of
R1766	T4027	T4026	amod	knock,mouse
R1767	T4028	T4027	prt	out,knock
R1768	T4029	T4020	nsubj	we,detected
R1769	T4030	T4031	det	a,membrane
R1770	T4031	T4020	dobj	membrane,detected
R1771	T4032	T4031	amod	thickened,membrane
R1772	T4033	T4031	amod	basal,membrane
R1773	T4034	T4031	cc	and,membrane
R1774	T4035	T4036	det	a,space
R1775	T4036	T4031	conj	space,membrane
R1776	T4037	T4036	amod	widened,space
R1777	T4038	T4036	amod	intercellular,space
R1778	T4039	T4020	punct	.,detected
R1779	T4041	T4042	nsubj	This,cause
R1780	T4043	T4042	aux	may,cause
R1781	T4044	T4045	amod	unspecific,binding
R1782	T4045	T4042	dobj	binding,cause
R1783	T4046	T4045	prep	of,binding
R1784	T4047	T4048	det	the,antibody
R1785	T4048	T4046	pobj	antibody,of
R1786	T4049	T4048	amod	secondary,antibody
R1787	T4050	T4042	punct	.,cause
R1788	T4052	T4053	det	An,staining
R1789	T4053	T4055	nsubjpass	staining,found
R1790	T4054	T4053	amod	equal,staining
R1791	T4056	T4053	prep	of,staining
R1792	T4057	T4058	compound	Annexin,A7
R1793	T4058	T4056	pobj	A7,of
R1794	T4059	T4055	auxpass	was,found
R1795	T4060	T4055	advmod	also,found
R1796	T4061	T4055	prep	in,found
R1797	T4062	T4063	det	the,cerebellum
R1798	T4063	T4061	pobj	cerebellum,in
R1799	T4064	T4065	punct	(,5A
R1800	T4065	T4055	parataxis	5A,found
R1801	T4066	T4065	compound	Fig.,5A
R1802	T4067	T4065	punct	),5A
R1803	T4068	T4055	punct	.,found
R1804	T4070	T4071	nsubj	Nuclei,show
R1805	T4072	T4070	prep	of,Nuclei
R1806	T4073	T4072	pobj	neurons,of
R1807	T4074	T4073	prep	in,neurons
R1808	T4075	T4076	det	the,granulosum
R1809	T4076	T4074	pobj	granulosum,in
R1810	T4077	T4076	compound	stratum,granulosum
R1811	T4078	T4079	det	the,staining
R1812	T4079	T4071	dobj	staining,show
R1813	T4080	T4081	advmod	most,prominent
R1814	T4081	T4079	amod	prominent,staining
R1815	T4082	T4083	punct	(,D
R1816	T4083	T4071	parataxis	D,show
R1817	T4084	T4083	nmod	Fig.,D
R1818	T4085	T4083	nmod	5C,D
R1819	T4086	T4083	punct	",",D
R1820	T4087	T4083	punct	),D
R1821	T4088	T4071	punct	.,show
R1822	T4090	T4091	prep	In,appear
R1823	T4092	T4093	det	the,moleculare
R1824	T4093	T4090	pobj	moleculare,In
R1825	T4094	T4093	compound	stratum,moleculare
R1826	T4095	T4093	punct	", ",moleculare
R1827	T4096	T4097	dep	which,is
R1828	T4097	T4093	relcl	is,moleculare
R1829	T4098	T4097	acomp	poor,is
R1830	T4099	T4097	prep	in,is
R1831	T4100	T4101	compound	cell,bodies
R1832	T4101	T4099	pobj	bodies,in
R1833	T4102	T4101	prep	of,bodies
R1834	T4103	T4102	pobj	neurons,of
R1835	T4104	T4091	punct	", ",appear
R1836	T4105	T4106	advmod	only,dots
R1837	T4106	T4091	nsubj	dots,appear
R1838	T4107	T4106	det	a,dots
R1839	T4108	T4106	amod	few,dots
R1840	T4109	T4110	dep	which,correspond
R1841	T4110	T4091	ccomp	correspond,appear
R1842	T4111	T4110	advmod	also,correspond
R1843	T4112	T4110	prep	to,correspond
R1844	T4113	T4112	pobj	nuclei,to
R1845	T4114	T4113	prep	of,nuclei
R1846	T4115	T4114	pobj	neurons,of
R1847	T4116	T4091	punct	.,appear
R1848	T4118	T4119	nsubj	Astrocytes,are
R1849	T4120	T4119	advmod	also,are
R1850	T4121	T4119	acomp	positive,are
R1851	T4122	T4121	prep	for,positive
R1852	T4123	T4124	compound	Annexin,A7
R1853	T4124	T4122	pobj	A7,for
R1854	T4125	T4119	prep	in,are
R1855	T4126	T4127	det	the,nuclei
R1856	T4127	T4125	pobj	nuclei,in
R1857	T4128	T4127	cc	and,nuclei
R1858	T4129	T4127	conj	cytoplasm,nuclei
R1859	T4130	T4131	punct	(,shown
R1860	T4131	T4119	parataxis	shown,are
R1861	T4132	T4131	nsubj	data,shown
R1862	T4133	T4131	neg	not,shown
R1863	T4134	T4131	punct	),shown
R1864	T4135	T4119	punct	.,are
R1865	T4137	T4138	det	The,signal
R1866	T4138	T4139	nsubj	signal,are
R1867	T4140	T4138	prep	of,signal
R1868	T4141	T4142	det	the,border
R1869	T4142	T4140	pobj	border,of
R1870	T4143	T4142	amod	pial,border
R1871	T4144	T4138	cc	and,signal
R1872	T4145	T4146	det	the,stain
R1873	T4146	T4138	conj	stain,signal
R1874	T4147	T4146	amod	prominent,stain
R1875	T4148	T4146	prep	of,stain
R1876	T4149	T4150	det	the,tracts
R1877	T4150	T4148	pobj	tracts,of
R1878	T4151	T4152	amod	white,matter
R1879	T4152	T4150	compound	matter,tracts
R1880	T4153	T4150	punct	(,tracts
R1881	T4154	T4155	compound	laminae,medullares
R1882	T4155	T4150	appos	medullares,tracts
R1883	T4156	T4139	punct	),are
R1884	T4157	T4139	prep	due,are
R1885	T4158	T4157	pcomp	to,due
R1886	T4159	T4160	compound	Annexin,A7
R1887	T4160	T4161	npadvmod	A7,positive
R1888	T4161	T4162	amod	positive,astrocytes
R1889	T4162	T4157	pobj	astrocytes,due
R1890	T4163	T4164	punct	(,A
R1891	T4164	T4139	parataxis	A,are
R1892	T4165	T4164	nmod	Fig.,A
R1893	T4166	T4164	nummod	5,A
R1894	T4167	T4168	punct	–,C
R1895	T4168	T4164	prep	C,A
R1896	T4169	T4139	punct	),are
R1897	T4170	T4139	punct	.,are
R1898	T4172	T4173	amod	Higher,magnification
R1899	T4173	T4174	nsubj	magnification,revealed
R1900	T4175	T4173	prep	of,magnification
R1901	T4176	T4177	det	the,layer
R1902	T4177	T4175	pobj	layer,of
R1903	T4178	T4177	amod	boundary,layer
R1904	T4179	T4177	prep	between,layer
R1905	T4180	T4181	compound	stratum,moleculare
R1906	T4181	T4179	pobj	moleculare,between
R1907	T4182	T4181	cc	and,moleculare
R1908	T4183	T4181	conj	granulosum,moleculare
R1909	T4184	T4185	punct	(,5D
R1910	T4185	T4173	parataxis	5D,magnification
R1911	T4186	T4185	compound	Fig.,5D
R1912	T4187	T4185	punct	),5D
R1913	T4188	T4189	det	an,signal
R1914	T4189	T4174	dobj	signal,revealed
R1915	T4190	T4191	compound	Annexin,A7
R1916	T4191	T4189	compound	A7,signal
R1917	T4192	T4189	prep	in,signal
R1918	T4193	T4192	pobj	nuclei,in
R1919	T4194	T4192	punct	", ",in
R1920	T4195	T4192	cc	but,in
R1921	T4196	T4195	advmod	also,but
R1922	T4197	T4192	conj	in,in
R1923	T4198	T4199	det	the,cytoplasm
R1924	T4199	T4197	pobj	cytoplasm,in
R1925	T4200	T4197	cc	and,in
R1926	T4201	T4197	conj	at,in
R1927	T4202	T4203	det	the,membrane
R1928	T4203	T4201	pobj	membrane,at
R1929	T4204	T4203	compound	plasma,membrane
R1930	T4205	T4203	prep	of,membrane
R1931	T4206	T4207	compound	Purkinje,cells
R1932	T4207	T4205	pobj	cells,of
R1933	T4208	T4209	punct	(,5E
R1934	T4209	T4174	parataxis	5E,revealed
R1935	T4210	T4209	compound	Fig.,5E
R1936	T4211	T4209	punct	),5E
R1937	T4212	T4174	punct	.,revealed
R1938	T4214	T4215	det	The,pair
R1939	T4215	T4217	nsubj	pair,points
R1940	T4216	T4215	amod	typical,pair
R1941	T4218	T4215	prep	of,pair
R1942	T4219	T4218	pobj	dendrites,of
R1943	T4220	T4217	prep	to,points
R1944	T4221	T4222	det	the,margin
R1945	T4222	T4220	pobj	margin,to
R1946	T4223	T4222	prep	of,margin
R1947	T4224	T4225	det	the,cortex
R1948	T4225	T4223	pobj	cortex,of
R1949	T4226	T4225	amod	cerebellar,cortex
R1950	T4227	T4217	punct	.,points
R1951	T4229	T4230	det	The,specificity
R1952	T4230	T4231	nsubjpass	specificity,confirmed
R1953	T4232	T4230	prep	of,specificity
R1954	T4233	T4234	det	the,signal
R1955	T4234	T4232	pobj	signal,of
R1956	T4235	T4236	compound	Annexin,A7
R1957	T4236	T4234	compound	A7,signal
R1958	T4237	T4231	auxpass	was,confirmed
R1959	T4238	T4231	advmod	further,confirmed
R1960	T4239	T4231	prep	in,confirmed
R1961	T4240	T4241	amod	similar,sections
R1962	T4241	T4239	pobj	sections,in
R1963	T4242	T4241	compound	brain,sections
R1964	T4243	T4241	prep	of,sections
R1965	T4244	T4245	det	the,mouse
R1966	T4245	T4243	pobj	mouse,of
R1967	T4246	T4245	nmod	AnxA7,mouse
R1968	T4247	T4246	punct	-,AnxA7
R1969	T4248	T4246	punct	/,AnxA7
R1970	T4249	T4246	punct	-,AnxA7
R1971	T4250	T4251	punct	(,5F
R1972	T4251	T4231	parataxis	5F,confirmed
R1973	T4252	T4251	compound	Fig.,5F
R1974	T4253	T4251	punct	),5F
R1975	T4254	T4231	punct	.,confirmed
R1976	T4256	T4257	compound	Fig.,5G
R1977	T4257	T4258	nsubj	5G,shows
R1978	T4259	T4260	compound	Annexin,A7
R1979	T4260	T4258	dobj	A7,shows
R1980	T4261	T4258	prep	in,shows
R1981	T4262	T4261	pobj	neurites,in
R1982	T4263	T4262	punct	(,neurites
R1983	T4264	T4262	appos	axons,neurites
R1984	T4265	T4262	punct	),neurites
R1985	T4266	T4262	acl	connecting,neurites
R1986	T4267	T4268	det	the,medullares
R1987	T4268	T4266	dobj	medullares,connecting
R1988	T4269	T4268	compound	laminae,medullares
R1989	T4270	T4266	prep	with,connecting
R1990	T4271	T4272	det	the,layer
R1991	T4272	T4270	pobj	layer,with
R1992	T4273	T4274	compound	Purkinje,cell
R1993	T4274	T4272	compound	cell,layer
R1994	T4275	T4274	punct	-,cell
R1995	T4276	T4258	punct	.,shows
R1996	T4278	T4279	advmod	Most,likely
R1997	T4279	T4280	advmod	likely,are
R1998	T4281	T4280	nsubj	these,are
R1999	T4282	T4283	det	the,axons
R2000	T4283	T4280	attr	axons,are
R2001	T4284	T4283	prep	of,axons
R2002	T4285	T4286	det	the,cells
R2003	T4286	T4284	pobj	cells,of
R2004	T4287	T4286	compound	Purkinje,cells
R2005	T4288	T4286	punct	-,cells
R2006	T4289	T4280	punct	.,are
R2007	T4291	T4292	advmod	Apart,contain
R2008	T4293	T4291	prep	from,Apart
R2009	T4294	T4295	det	these,neurites
R2010	T4295	T4293	pobj	neurites,from
R2011	T4296	T4295	amod	efferent,neurites
R2012	T4297	T4298	det	the,medullares
R2013	T4298	T4292	nsubj	medullares,contain
R2014	T4299	T4298	compound	laminae,medullares
R2015	T4300	T4301	punct	(,matter
R2016	T4301	T4298	parataxis	matter,medullares
R2017	T4302	T4303	compound	lamina,alba
R2018	T4303	T4301	dep	alba,matter
R2019	T4304	T4301	punct	", ",matter
R2020	T4305	T4301	amod	white,matter
R2021	T4306	T4301	punct	),matter
R2022	T4307	T4308	amod	afferent,fibers
R2023	T4308	T4292	dobj	fibers,contain
R2024	T4309	T4308	amod	mossy,fibers
R2025	T4310	T4309	cc	and,mossy
R2026	T4311	T4309	conj	climbing,mossy
R2027	T4312	T4292	punct	.,contain
R2028	T4314	T4315	advmod	Thus,arises
R2029	T4316	T4315	punct	", ",arises
R2030	T4317	T4318	det	the,stain
R2031	T4318	T4315	nsubj	stain,arises
R2032	T4319	T4318	amod	intense,stain
R2033	T4320	T4318	prep	of,stain
R2034	T4321	T4322	det	the,matter
R2035	T4322	T4320	pobj	matter,of
R2036	T4323	T4322	amod	cerebellar,matter
R2037	T4324	T4322	amod	white,matter
R2038	T4325	T4315	prep	from,arises
R2039	T4326	T4327	compound	Annexin,A7
R2040	T4327	T4325	pobj	A7,from
R2041	T4328	T4327	acl	located,A7
R2042	T4329	T4328	prep	in,located
R2043	T4330	T4329	pobj	astrocytes,in
R2044	T4331	T4330	cc	and,astrocytes
R2045	T4332	T4330	conj	neurites,astrocytes
R2046	T4333	T4315	punct	.,arises
R2047	T4423	T4422	prep	of,Expression
R2048	T4424	T4425	compound	Annexin,A7
R2049	T4425	T4423	pobj	A7,of
R2050	T4426	T4422	prep	in,Expression
R2051	T4427	T4428	det	the,isocortex
R2052	T4428	T4426	pobj	isocortex,in
R2053	T4429	T4430	amod	adult,human
R2054	T4430	T4428	amod	human,isocortex
R2055	T4432	T4433	prep	In,exhibited
R2056	T4434	T4435	det	the,neocortex
R2057	T4435	T4432	pobj	neocortex,In
R2058	T4436	T4435	amod	human,neocortex
R2059	T4437	T4435	amod	parietal,neocortex
R2060	T4438	T4435	prep	of,neocortex
R2061	T4439	T4440	amod	aged,individuals
R2062	T4440	T4438	pobj	individuals,of
R2063	T4441	T4440	prep	without,individuals
R2064	T4442	T4443	det	any,alterations
R2065	T4443	T4441	pobj	alterations,without
R2066	T4444	T4443	amod	neuropathological,alterations
R2067	T4445	T4433	punct	", ",exhibited
R2068	T4446	T4447	amod	subpial,astrocytes
R2069	T4447	T4433	nsubj	astrocytes,exhibited
R2070	T4448	T4449	det	a,staining
R2071	T4449	T4433	dobj	staining,exhibited
R2072	T4450	T4449	prep	of,staining
R2073	T4451	T4452	compound	Annexin,A7
R2074	T4452	T4450	pobj	A7,of
R2075	T4453	T4433	prep	in,exhibited
R2076	T4454	T4455	det	the,cytoplasm
R2077	T4455	T4453	pobj	cytoplasm,in
R2078	T4456	T4433	punct	.,exhibited
R2079	T4458	T4459	det	A,presence
R2080	T4459	T4461	nsubjpass	presence,limited
R2081	T4460	T4459	amod	nuclear,presence
R2082	T4462	T4459	prep	of,presence
R2083	T4463	T4464	compound	Annexin,A7
R2084	T4464	T4462	pobj	A7,of
R2085	T4465	T4461	auxpass	was,limited
R2086	T4466	T4461	prep	to,limited
R2087	T4467	T4468	amod	single,astrocytes
R2088	T4468	T4466	pobj	astrocytes,to
R2089	T4469	T4470	punct	(,6B
R2090	T4470	T4461	parataxis	6B,limited
R2091	T4471	T4470	compound	Fig.,6B
R2092	T4472	T4470	punct	),6B
R2093	T4473	T4461	punct	.,limited
R2094	T4475	T4476	amod	Pyramidal,neurons
R2095	T4476	T4477	nsubj	neurons,exhibited
R2096	T4478	T4476	punct	", ",neurons
R2097	T4479	T4480	advmod	predominantly,those
R2098	T4480	T4476	appos	those,neurons
R2099	T4481	T4480	prep	of,those
R2100	T4482	T4481	pobj	layer,of
R2101	T4483	T4482	nummod	V,layer
R2102	T4484	T4485	compound	Annexin,A7
R2103	T4485	T4477	dobj	A7,exhibited
R2104	T4486	T4477	prep	at,exhibited
R2105	T4487	T4488	det	the,membrane
R2106	T4488	T4486	pobj	membrane,at
R2107	T4489	T4488	compound	plasma,membrane
R2108	T4490	T4488	prep	of,membrane
R2109	T4491	T4492	poss	their,perikaryon
R2110	T4492	T4490	pobj	perikaryon,of
R2111	T4493	T4494	advmod	as,as
R2112	T4494	T4490	cc	as,of
R2113	T4495	T4494	advmod	well,as
R2114	T4496	T4490	conj	of,of
R2115	T4497	T4498	det	the,dendrite
R2116	T4498	T4496	pobj	dendrite,of
R2117	T4499	T4498	amod	apical,dendrite
R2118	T4500	T4501	punct	(,C
R2119	T4501	T4477	parataxis	C,exhibited
R2120	T4502	T4501	nmod	Fig.,C
R2121	T4503	T4501	nmod	6A,C
R2122	T4504	T4501	punct	",",C
R2123	T4505	T4501	punct	),C
R2124	T4506	T4477	punct	.,exhibited
R2125	T4508	T4509	det	The,neurons
R2126	T4509	T4510	nsubj	neurons,lacked
R2127	T4511	T4512	det	a,signal
R2128	T4512	T4510	dobj	signal,lacked
R2129	T4513	T4512	prep	for,signal
R2130	T4514	T4515	compound	Annexin,A7
R2131	T4515	T4513	pobj	A7,for
R2132	T4516	T4510	prep	in,lacked
R2133	T4517	T4518	poss	their,nuclei
R2134	T4518	T4516	pobj	nuclei,in
R2135	T4519	T4510	punct	.,lacked
R2136	T4521	T4522	amod	Apical,dendrites
R2137	T4522	T4523	nsubj	dendrites,indicated
R2138	T4524	T4522	prep	within,dendrites
R2139	T4525	T4526	det	the,layer
R2140	T4526	T4524	pobj	layer,within
R2141	T4527	T4526	amod	molecular,layer
R2142	T4528	T4523	advmod	also,indicated
R2143	T4529	T4530	det	a,staining
R2144	T4530	T4523	dobj	staining,indicated
R2145	T4531	T4530	amod	positive,staining
R2146	T4532	T4530	prep	for,staining
R2147	T4533	T4534	compound	Annexin,A7
R2148	T4534	T4532	pobj	A7,for
R2149	T4535	T4536	punct	(,6B
R2150	T4536	T4523	parataxis	6B,indicated
R2151	T4537	T4536	compound	Fig.,6B
R2152	T4538	T4536	punct	),6B
R2153	T4539	T4523	punct	.,indicated
R2154	T4541	T4542	det	The,pattern
R2155	T4542	T4544	nsubj	pattern,change
R2156	T4543	T4542	compound	staining,pattern
R2157	T4545	T4542	prep	of,pattern
R2158	T4546	T4547	compound	Annexin,A7
R2159	T4547	T4545	pobj	A7,of
R2160	T4548	T4542	prep	in,pattern
R2161	T4549	T4550	det	the,brain
R2162	T4550	T4548	pobj	brain,in
R2163	T4551	T4550	amod	human,brain
R2164	T4552	T4550	compound	autopsy,brain
R2165	T4553	T4544	aux	did,change
R2166	T4554	T4544	neg	not,change
R2167	T4555	T4544	prep	after,change
R2168	T4556	T4555	pobj	pre-treatment,after
R2169	T4557	T4556	prep	with,pre-treatment
R2170	T4558	T4557	pobj	trypsin,with
R2171	T4559	T4544	punct	.,change
R2180	T5137	T5136	prep	of,Presence
R2181	T5138	T5139	compound	Annexin,A7
R2182	T5139	T5137	pobj	A7,of
R2183	T5140	T5136	prep	in,Presence
R2184	T5141	T5140	pobj	nuclei,in
R2185	T5142	T5141	prep	from,nuclei
R2186	T5143	T5144	amod	neuronal,cells
R2187	T5144	T5142	pobj	cells,from
R2188	T5145	T5143	cc	and,neuronal
R2189	T5146	T5143	conj	astroglial,neuronal
R2190	T5148	T5149	det	The,localisation
R2191	T5149	T5151	nsubjpass	localisation,detected
R2192	T5150	T5149	amod	nuclear,localisation
R2193	T5152	T5149	prep	of,localisation
R2194	T5153	T5154	compound	Annexin,A7
R2195	T5154	T5152	pobj	A7,of
R2196	T5155	T5149	prep	in,localisation
R2197	T5156	T5155	pobj	mice,in
R2198	T5157	T5149	acl	observed,localisation
R2199	T5158	T5157	prep	in,observed
R2200	T5159	T5160	amod	immature,cells
R2201	T5160	T5158	pobj	cells,in
R2202	T5161	T5160	prep	at,cells
R2203	T5162	T5161	pobj	E16,at
R2204	T5163	T5160	cc	and,cells
R2205	T5164	T5165	amod	differentiated,neurons
R2206	T5165	T5160	conj	neurons,cells
R2207	T5166	T5165	amod	adult,neurons
R2208	T5167	T5151	aux	could,detected
R2209	T5168	T5151	auxpass	be,detected
R2210	T5169	T5151	advmod	only,detected
R2211	T5170	T5151	prep	as,detected
R2212	T5171	T5172	det	a,signal
R2213	T5172	T5170	pobj	signal,as
R2214	T5173	T5172	amod	strong,signal
R2215	T5174	T5175	advmod	when,pre-treated
R2216	T5175	T5151	advcl	pre-treated,detected
R2217	T5176	T5177	det	the,sections
R2218	T5177	T5175	nsubjpass	sections,pre-treated
R2219	T5178	T5177	compound	brain,sections
R2220	T5179	T5175	auxpass	were,pre-treated
R2221	T5180	T5175	prep	with,pre-treated
R2222	T5181	T5180	pobj	trypsin,with
R2223	T5182	T5175	prep	before,pre-treated
R2224	T5183	T5184	compound	antibody,staining
R2225	T5184	T5182	pobj	staining,before
R2226	T5185	T5151	punct	.,detected
R2227	T5187	T5188	det	This,procedure
R2228	T5188	T5189	nsubjpass	procedure,thought
R2229	T5190	T5189	auxpass	is,thought
R2230	T5191	T5192	aux	to,allow
R2231	T5192	T5189	xcomp	allow,thought
R2232	T5193	T5194	det	the,antibodies
R2233	T5194	T5195	nsubj	antibodies,access
R2234	T5195	T5192	ccomp	access,allow
R2235	T5196	T5195	aux	to,access
R2236	T5197	T5195	dobj	epitopes,access
R2237	T5198	T5197	acl	masked,epitopes
R2238	T5199	T5198	agent	by,masked
R2239	T5200	T5201	det	the,fixation
R2240	T5201	T5199	pobj	fixation,by
R2241	T5202	T5201	compound	formaldehyde,fixation
R2242	T5203	T5189	punct	.,thought
R2243	T5205	T5206	compound	Methanol,fixation
R2244	T5206	T5207	nsubj	fixation,led
R2245	T5208	T5206	prep	of,fixation
R2246	T5209	T5210	amod	cultured,cells
R2247	T5210	T5208	pobj	cells,of
R2248	T5211	T5207	prep	to,led
R2249	T5212	T5213	amod	inconsistent,results
R2250	T5213	T5211	pobj	results,to
R2251	T5214	T5207	punct	.,led
R2252	T5216	T5217	compound	Antigen,retrieval
R2253	T5217	T5218	nsubj	retrieval,employs
R2254	T5219	T5217	prep	in,retrieval
R2255	T5220	T5221	npadvmod	formalin,fixed
R2256	T5221	T5223	amod	fixed,sections
R2257	T5222	T5221	punct	-,fixed
R2258	T5223	T5219	pobj	sections,in
R2259	T5224	T5221	cc	and,fixed
R2260	T5225	T5226	npadvmod	paraffin,embedded
R2261	T5226	T5221	conj	embedded,fixed
R2262	T5227	T5226	punct	-,embedded
R2263	T5228	T5223	compound	tissue,sections
R2264	T5229	T5230	amod	various,methods
R2265	T5230	T5218	dobj	methods,employs
R2266	T5231	T5230	nmod	heating,methods
R2267	T5232	T5231	cc	or,heating
R2268	T5233	T5234	amod	proteolytic,pre-treatment
R2269	T5234	T5231	conj	pre-treatment,heating
R2270	T5235	T5218	punct	[,employs
R2271	T5236	T5237	punct	[,15
R2272	T5237	T5218	parataxis	15,employs
R2273	T5238	T5239	punct	-,17
R2274	T5239	T5237	prep	17,15
R2275	T5240	T5237	punct	],15
R2276	T5241	T5242	punct	;,Ein
R2277	T5242	T5218	meta	Ein,employs
R2278	T5243	T5242	nmod	Handbuch,Ein
R2279	T5244	T5242	nmod	für,Ein
R2280	T5245	T5242	nmod	die,Ein
R2281	T5246	T5242	nmod	Histologie,Ein
R2282	T5247	T5242	punct	", ",Ein
R2283	T5248	T5242	nmod	dianova,Ein
R2284	T5249	T5242	nmod	GmbH,Ein
R2285	T5250	T5242	punct	", ",Ein
R2286	T5251	T5242	nmod	Hamburg,Ein
R2287	T5252	T5242	punct	", ",Ein
R2288	T5253	T5242	nmod	Germany,Ein
R2289	T5254	T5242	punct	],Ein
R2290	T5255	T5218	punct	.,employs
R2291	T5257	T5258	det	These,methods
R2292	T5258	T5259	nsubj	methods,result
R2293	T5260	T5259	aux	can,result
R2294	T5261	T5259	prep	in,result
R2295	T5262	T5263	amod	moderate,staining
R2296	T5263	T5261	pobj	staining,in
R2297	T5264	T5262	cc	or,moderate
R2298	T5265	T5262	conj	strong,moderate
R2299	T5266	T5263	amod	specific,staining
R2300	T5267	T5263	compound	antibody,staining
R2301	T5268	T5259	punct	", ",result
R2302	T5269	T5259	cc	but,result
R2303	T5270	T5271	det	the,detectability
R2304	T5271	T5272	nsubjpass	detectability,decreased
R2305	T5272	T5259	conj	decreased,result
R2306	T5273	T5271	prep	of,detectability
R2307	T5274	T5275	amod	other,antigens
R2308	T5275	T5273	pobj	antigens,of
R2309	T5276	T5272	aux	might,decreased
R2310	T5277	T5272	auxpass	be,decreased
R2311	T5278	T5272	punct	.,decreased
R2312	T5280	T5281	advmod	Moreover,has
R2313	T5282	T5281	punct	", ",has
R2314	T5283	T5284	det	the,pre-treatment
R2315	T5284	T5281	nsubj	pre-treatment,has
R2316	T5285	T5284	amod	optimal,pre-treatment
R2317	T5286	T5287	aux	to,individualised
R2318	T5287	T5281	xcomp	individualised,has
R2319	T5288	T5287	auxpass	be,individualised
R2320	T5289	T5287	prep	for,individualised
R2321	T5290	T5291	det	each,antigen
R2322	T5291	T5289	pobj	antigen,for
R2323	T5292	T5281	punct	.,has
R2324	T5294	T5295	aux	To,verify
R2325	T5295	T5296	advcl	verify,used
R2326	T5297	T5298	det	the,presence
R2327	T5298	T5295	dobj	presence,verify
R2328	T5299	T5298	prep	of,presence
R2329	T5300	T5301	amod	endogenous,A7
R2330	T5301	T5299	pobj	A7,of
R2331	T5302	T5301	compound	Annexin,A7
R2332	T5303	T5295	prep	in,verify
R2333	T5304	T5303	pobj	nuclei,in
R2334	T5305	T5296	punct	", ",used
R2335	T5306	T5296	nsubj	we,used
R2336	T5307	T5308	det	a,approach
R2337	T5308	T5296	dobj	approach,used
R2338	T5309	T5308	amod	biochemical,approach
R2339	T5310	T5296	cc	and,used
R2340	T5311	T5296	conj	purified,used
R2341	T5312	T5311	dobj	nuclei,purified
R2342	T5313	T5311	prep	from,purified
R2343	T5314	T5315	nmod	neuro,cells
R2344	T5315	T5313	pobj	cells,from
R2345	T5316	T5314	punct	-,neuro
R2346	T5317	T5314	nummod	2a,neuro
R2347	T5318	T5314	punct	", ",neuro
R2348	T5319	T5314	conj	PC,neuro
R2349	T5320	T5319	punct	-,PC
R2350	T5321	T5319	nummod	12,PC
R2351	T5322	T5319	punct	", ",PC
R2352	T5323	T5319	cc	and,PC
R2353	T5324	T5319	conj	C6,PC
R2354	T5325	T5296	cc	and,used
R2355	T5326	T5296	conj	treated,used
R2356	T5327	T5326	dobj	them,treated
R2357	T5328	T5326	prep	with,treated
R2358	T5329	T5330	det	a,buffer
R2359	T5330	T5328	pobj	buffer,with
R2360	T5331	T5330	amod	hypotonic,buffer
R2361	T5332	T5330	compound	extraction,buffer
R2362	T5333	T5334	aux	to,obtain
R2363	T5334	T5326	advcl	obtain,treated
R2364	T5335	T5336	det	the,nucleoplasm
R2365	T5336	T5334	dobj	nucleoplasm,obtain
R2366	T5337	T5338	punct	(,7A
R2367	T5338	T5326	parataxis	7A,treated
R2368	T5339	T5338	compound	Fig.,7A
R2369	T5340	T5338	punct	),7A
R2370	T5341	T5296	punct	.,used
R2371	T5343	T5344	det	The,nucleoplasm
R2372	T5344	T5345	nsubjpass	nucleoplasm,subjected
R2373	T5346	T5344	cc	and,nucleoplasm
R2374	T5347	T5348	det	the,nuclei
R2375	T5348	T5344	conj	nuclei,nucleoplasm
R2376	T5349	T5348	amod	remaining,nuclei
R2377	T5350	T5344	punct	", ",nucleoplasm
R2378	T5351	T5352	prep	from,extracted
R2379	T5352	T5344	relcl	extracted,nucleoplasm
R2380	T5353	T5351	pobj	which,from
R2381	T5354	T5355	det	the,nucleoplasm
R2382	T5355	T5352	nsubjpass	nucleoplasm,extracted
R2383	T5356	T5352	aux	has,extracted
R2384	T5357	T5352	auxpass	been,extracted
R2385	T5358	T5352	advmod	partially,extracted
R2386	T5359	T5345	punct	", ",subjected
R2387	T5360	T5345	auxpass	were,subjected
R2388	T5361	T5345	prep	to,subjected
R2389	T5362	T5363	compound	SDS,PAGE
R2390	T5363	T5361	pobj	PAGE,to
R2391	T5364	T5363	punct	-,PAGE
R2392	T5365	T5363	cc	and,PAGE
R2393	T5366	T5367	compound	Western,blotting
R2394	T5367	T5363	conj	blotting,PAGE
R2395	T5368	T5345	punct	.,subjected
R2396	T5370	T5371	compound	Western,blots
R2397	T5371	T5372	nsubj	blots,showed
R2398	T5372	T5379	ccomp	showed,extracted
R2399	T5373	T5371	acl	probed,blots
R2400	T5374	T5373	prep	with,probed
R2401	T5375	T5376	nmod	mAb,203
R2402	T5376	T5374	pobj	203,with
R2403	T5377	T5378	punct	–,217
R2404	T5378	T5376	prep	217,203
R2405	T5380	T5381	compound	Annexin,A7
R2406	T5381	T5372	dobj	A7,showed
R2407	T5382	T5372	prep	in,showed
R2408	T5383	T5384	det	the,nucleoplasm
R2409	T5384	T5382	pobj	nucleoplasm,in
R2410	T5385	T5384	prep	of,nucleoplasm
R2411	T5386	T5387	amod	neuronal,lines
R2412	T5387	T5385	pobj	lines,of
R2413	T5388	T5389	punct	(,2a
R2414	T5389	T5386	parataxis	2a,neuronal
R2415	T5390	T5389	compound	neuro,2a
R2416	T5391	T5389	punct	-,2a
R2417	T5392	T5389	punct	", ",2a
R2418	T5393	T5389	appos	PC,2a
R2419	T5394	T5393	punct	-,PC
R2420	T5395	T5393	nummod	12,PC
R2421	T5396	T5389	punct	),2a
R2422	T5397	T5386	cc	and,neuronal
R2423	T5398	T5386	conj	astroglial,neuronal
R2424	T5399	T5400	punct	(,C6
R2425	T5400	T5398	parataxis	C6,astroglial
R2426	T5401	T5400	punct	),C6
R2427	T5402	T5387	compound	cell,lines
R2428	T5403	T5404	punct	(,7A
R2429	T5404	T5372	parataxis	7A,showed
R2430	T5405	T5404	compound	Fig.,7A
R2431	T5406	T5404	punct	),7A
R2432	T5407	T5379	punct	", ",extracted
R2433	T5408	T5379	advmod	however,extracted
R2434	T5409	T5379	punct	", ",extracted
R2435	T5410	T5411	det	the,isoform
R2436	T5411	T5379	nsubjpass	isoform,extracted
R2437	T5412	T5411	amod	large,isoform
R2438	T5413	T5379	aux	could,extracted
R2439	T5414	T5379	advmod	only,extracted
R2440	T5415	T5379	auxpass	be,extracted
R2441	T5416	T5379	advmod	clearly,extracted
R2442	T5417	T5379	prep	with,extracted
R2443	T5418	T5417	pobj	nucleoplasm,with
R2444	T5419	T5418	prep	from,nucleoplasm
R2445	T5420	T5421	compound	C6,cells
R2446	T5421	T5419	pobj	cells,from
R2447	T5422	T5379	punct	.,extracted
R2448	T5424	T5425	prep	For,used
R2449	T5426	T5424	pobj	control,For
R2450	T5427	T5425	punct	", ",used
R2451	T5428	T5425	nsubjpass	antibodies,used
R2452	T5429	T5428	prep	against,antibodies
R2453	T5430	T5429	pobj	LAP2α,against
R2454	T5431	T5430	punct	", ",LAP2α
R2455	T5432	T5430	conj	Emerin,LAP2α
R2456	T5433	T5432	cc	and,Emerin
R2457	T5434	T5432	conj	tubulin,Emerin
R2458	T5435	T5425	auxpass	were,used
R2459	T5436	T5437	aux	to,verify
R2460	T5437	T5425	advcl	verify,used
R2461	T5438	T5439	mark	that,extracted
R2462	T5439	T5437	ccomp	extracted,verify
R2463	T5440	T5441	det	the,nucleoplasm
R2464	T5441	T5439	nsubjpass	nucleoplasm,extracted
R2465	T5442	T5443	punct	(,LAP2α
R2466	T5443	T5441	parataxis	LAP2α,nucleoplasm
R2467	T5444	T5443	dep	marker,LAP2α
R2468	T5445	T5443	punct	:,LAP2α
R2469	T5446	T5443	punct	", ",LAP2α
R2470	T5447	T5448	dep	which,is
R2471	T5448	T5443	relcl	is,LAP2α
R2472	T5449	T5450	compound	anon,membrane
R2473	T5450	T5452	npadvmod	membrane,bound
R2474	T5451	T5450	punct	-,membrane
R2475	T5452	T5454	amod	bound,isoform
R2476	T5453	T5452	punct	-,bound
R2477	T5454	T5448	attr	isoform,is
R2478	T5455	T5454	prep	of,isoform
R2479	T5456	T5455	pobj	LAP2,of
R2480	T5457	T5443	punct	),LAP2α
R2481	T5458	T5439	auxpass	was,extracted
R2482	T5459	T5439	advmod	successfully,extracted
R2483	T5460	T5439	cc	and,extracted
R2484	T5461	T5460	advmod	also,and
R2485	T5462	T5463	neg	not,contaminated
R2486	T5463	T5439	conj	contaminated,extracted
R2487	T5464	T5463	prep	by,contaminated
R2488	T5465	T5466	amod	nuclear,membranes
R2489	T5466	T5464	pobj	membranes,by
R2490	T5467	T5468	punct	(,Emerin
R2491	T5468	T5466	parataxis	Emerin,membranes
R2492	T5469	T5468	dep	marker,Emerin
R2493	T5470	T5468	punct	:,Emerin
R2494	T5471	T5468	punct	),Emerin
R2495	T5472	T5464	cc	or,by
R2496	T5473	T5464	conj	by,by
R2497	T5474	T5473	pobj	cytoplasm,by
R2498	T5475	T5476	punct	(,tubulin
R2499	T5476	T5474	parataxis	tubulin,cytoplasm
R2500	T5477	T5476	dep	marker,tubulin
R2501	T5478	T5476	punct	:,tubulin
R2502	T5479	T5476	punct	),tubulin
R2503	T5480	T5425	punct	.,used
R2504	T5482	T5483	advmod	Likewise,observed
R2505	T5484	T5483	punct	", ",observed
R2506	T5485	T5483	prep	in,observed
R2507	T5486	T5487	det	these,lines
R2508	T5487	T5485	pobj	lines,in
R2509	T5488	T5487	amod	neuronal,lines
R2510	T5489	T5488	cc	and,neuronal
R2511	T5490	T5488	conj	astroglial,neuronal
R2512	T5491	T5487	compound	cell,lines
R2513	T5492	T5493	compound	Annexin,A7
R2514	T5493	T5483	nsubjpass	A7,observed
R2515	T5494	T5483	aux	had,observed
R2516	T5495	T5483	auxpass	been,observed
R2517	T5496	T5483	prep	in,observed
R2518	T5497	T5498	det	the,nucleus
R2519	T5498	T5496	pobj	nucleus,in
R2520	T5499	T5483	prep	by,observed
R2521	T5500	T5499	pobj	immunofluorescence,by
R2522	T5501	T5502	punct	(,7B
R2523	T5502	T5483	parataxis	7B,observed
R2524	T5503	T5502	compound	Fig.,7B
R2525	T5504	T5502	cc	and,7B
R2526	T5505	T5506	nsubj	data,shown
R2527	T5506	T5502	conj	shown,7B
R2528	T5507	T5506	neg	not,shown
R2529	T5508	T5506	punct	),shown
R2530	T5509	T5483	punct	.,observed
R2531	T5511	T5512	nsubj	We,noticed
R2532	T5512	T5513	ccomp	noticed,found
R2533	T5514	T5515	det	no,difference
R2534	T5515	T5512	dobj	difference,noticed
R2535	T5516	T5515	prep	between,difference
R2536	T5517	T5518	nmod	PC,cells
R2537	T5518	T5516	pobj	cells,between
R2538	T5519	T5517	punct	-,PC
R2539	T5520	T5517	nummod	12,PC
R2540	T5521	T5517	cc	and,PC
R2541	T5522	T5523	compound	neuro,2a
R2542	T5523	T5517	conj	2a,PC
R2543	T5524	T5523	punct	-,2a
R2544	T5525	T5513	punct	", ",found
R2545	T5526	T5513	advmod	however,found
R2546	T5527	T5513	punct	", ",found
R2547	T5528	T5513	prep	as,found
R2548	T5529	T5528	prep	for,as
R2549	T5530	T5531	det	the,sections
R2550	T5531	T5529	pobj	sections,for
R2551	T5532	T5531	compound	brain,sections
R2552	T5533	T5513	punct	", ",found
R2553	T5534	T5513	nsubj	we,found
R2554	T5535	T5536	det	an,increase
R2555	T5536	T5513	dobj	increase,found
R2556	T5537	T5536	prep	of,increase
R2557	T5538	T5539	det	the,staining
R2558	T5539	T5537	pobj	staining,of
R2559	T5540	T5541	compound	Annexin,A7
R2560	T5541	T5539	compound	A7,staining
R2561	T5542	T5513	prep	after,found
R2562	T5543	T5542	pobj	pre-treatment,after
R2563	T5544	T5543	prep	with,pre-treatment
R2564	T5545	T5544	pobj	trypsin,with
R2565	T5546	T5513	punct	.,found
R5559	T11655	T11654	prep	of,Expression
R5560	T11656	T11657	compound	Annexin,A7
R5561	T11657	T11655	pobj	A7,of
R5562	T11658	T11654	punct	.,Expression
R5563	T11660	T11661	punct	(,A
R5564	T11661	T11662	meta	A,analysis
R5565	T11663	T11661	punct	),A
R5566	T11664	T11665	compound	Northern,blot
R5567	T11665	T11662	compound	blot,analysis
R5568	T11666	T11662	prep	of,analysis
R5569	T11667	T11666	pobj	RNA,of
R5570	T11668	T11667	prep	from,RNA
R5571	T11669	T11670	compound	ES,cells
R5572	T11670	T11668	pobj	cells,from
R5573	T11671	T11670	punct	-,cells
R5574	T11672	T11670	cc	and,cells
R5575	T11673	T11674	nmod	embryos,E17
R5576	T11674	T11670	conj	E17,cells
R5577	T11675	T11674	nmod	E7,E17
R5578	T11676	T11674	punct	", ",E17
R5579	T11677	T11674	nmod	E11,E17
R5580	T11678	T11674	punct	", ",E17
R5581	T11679	T11674	nmod	E15,E17
R5582	T11680	T11674	punct	", ",E17
R5583	T11681	T11667	acl	probed,RNA
R5584	T11682	T11681	prep	with,probed
R5585	T11683	T11684	det	a,cDNA
R5586	T11684	T11682	pobj	cDNA,with
R5587	T11685	T11686	amod	full,length
R5588	T11686	T11684	compound	length,cDNA
R5589	T11687	T11688	compound	annexin,A7
R5590	T11688	T11684	compound	A7,cDNA
R5591	T11689	T11662	punct	.,analysis
R5592	T11691	T11692	nsubjpass	Transcripts,detected
R5593	T11693	T11691	prep	of,Transcripts
R5594	T11694	T11695	nummod	1.8,kb
R5595	T11695	T11693	pobj	kb,of
R5596	T11696	T11694	cc	and,1.8
R5597	T11697	T11694	conj	2.4,1.8
R5598	T11698	T11692	auxpass	are,detected
R5599	T11699	T11692	prep	in,detected
R5600	T11700	T11701	det	all,stages
R5601	T11701	T11699	pobj	stages,in
R5602	T11702	T11701	acl	examined,stages
R5603	T11703	T11704	punct	(,panel
R5604	T11704	T11692	parataxis	panel,detected
R5605	T11705	T11704	amod	upper,panel
R5606	T11706	T11704	punct	),panel
R5607	T11707	T11692	punct	.,detected
R5608	T11709	T11710	nsubj	Reprobing,verified
R5609	T11711	T11709	prep	with,Reprobing
R5610	T11712	T11713	det	a,probe
R5611	T11713	T11711	pobj	probe,with
R5612	T11714	T11715	compound	β,actin
R5613	T11715	T11713	compound	actin,probe
R5614	T11716	T11715	punct	-,actin
R5615	T11717	T11718	amod	equal,loading
R5616	T11718	T11710	dobj	loading,verified
R5617	T11719	T11720	punct	(,band
R5618	T11720	T11710	parataxis	band,verified
R5619	T11721	T11722	amod	lower,panel
R5620	T11722	T11720	dep	panel,band
R5621	T11723	T11720	punct	", ",band
R5622	T11724	T11725	nummod	2.0,kb
R5623	T11725	T11720	compound	kb,band
R5624	T11726	T11720	punct	),band
R5625	T11727	T11710	punct	.,verified
R5626	T11729	T11730	punct	(,B
R5627	T11730	T11731	meta	B,express
R5628	T11732	T11730	punct	),B
R5629	T11733	T11734	amod	Murine,cells
R5630	T11734	T11731	nsubj	cells,express
R5631	T11735	T11734	compound	ES,cells
R5632	T11736	T11734	punct	(,cells
R5633	T11737	T11734	appos	ES,cells
R5634	T11738	T11731	punct	),express
R5635	T11739	T11740	advmod	only,isoform
R5636	T11740	T11731	dobj	isoform,express
R5637	T11741	T11740	det	the,isoform
R5638	T11742	T11740	amod	small,isoform
R5639	T11743	T11744	compound	Annexin,A7
R5640	T11744	T11740	compound	A7,isoform
R5641	T11745	T11731	punct	.,express
R5642	T11747	T11748	det	Both,isoforms
R5643	T11748	T11749	nsubjpass	isoforms,expressed
R5644	T11750	T11749	auxpass	are,expressed
R5645	T11751	T11749	prep	in,expressed
R5646	T11752	T11753	compound	neuroblastoma,cells
R5647	T11753	T11751	pobj	cells,in
R5648	T11754	T11755	compound	neuro,2a
R5649	T11755	T11753	compound	2a,cells
R5650	T11756	T11755	punct	-,2a
R5651	T11757	T11753	punct	(,cells
R5652	T11758	T11753	appos	N2A,cells
R5653	T11759	T11753	punct	),cells
R5654	T11760	T11753	punct	", ",cells
R5655	T11761	T11753	conj	heart,cells
R5656	T11762	T11761	punct	(,heart
R5657	T11763	T11761	appos	HRT,heart
R5658	T11764	T11761	punct	),heart
R5659	T11765	T11761	cc	and,heart
R5660	T11766	T11767	compound	brain,tissue
R5661	T11767	T11761	conj	tissue,heart
R5662	T11768	T11767	punct	(,tissue
R5663	T11769	T11767	appos	CNS,tissue
R5664	T11770	T11753	punct	),cells
R5665	T11771	T11753	prep	from,cells
R5666	T11772	T11773	amod	adult,mice
R5667	T11773	T11771	pobj	mice,from
R5668	T11774	T11749	punct	.,expressed
R5669	T11776	T11777	nsubjpass	Proteins,resolved
R5670	T11778	T11776	prep	from,Proteins
R5671	T11779	T11780	compound	neuro,2a
R5672	T11780	T11778	pobj	2a,from
R5673	T11781	T11780	punct	-,2a
R5674	T11782	T11780	cc	and,2a
R5675	T11783	T11780	conj	ES,2a
R5676	T11784	T11780	appos	cells,2a
R5677	T11785	T11784	punct	", ",cells
R5678	T11786	T11784	conj	heart,cells
R5679	T11787	T11786	cc	and,heart
R5680	T11788	T11786	conj	brain,heart
R5681	T11789	T11777	auxpass	were,resolved
R5682	T11790	T11777	prep	by,resolved
R5683	T11791	T11792	compound	SDS,electrophoresis
R5684	T11792	T11790	pobj	electrophoresis,by
R5685	T11793	T11794	compound	polyacrylamide,gel
R5686	T11794	T11792	compound	gel,electrophoresis
R5687	T11795	T11796	punct	(,acrylamide
R5688	T11796	T11777	parataxis	acrylamide,resolved
R5689	T11797	T11798	nummod	12,%
R5690	T11798	T11796	compound	%,acrylamide
R5691	T11799	T11796	punct	),acrylamide
R5692	T11800	T11777	cc	and,resolved
R5693	T11801	T11777	conj	transferred,resolved
R5694	T11802	T11801	prep	to,transferred
R5695	T11803	T11804	compound	nitrocellulose,membranes
R5696	T11804	T11802	pobj	membranes,to
R5697	T11805	T11777	punct	.,resolved
R5698	T11807	T11808	det	The,blot
R5699	T11808	T11810	nsubjpass	blot,probed
R5700	T11809	T11808	compound	Western,blot
R5701	T11811	T11810	auxpass	was,probed
R5702	T11812	T11810	prep	with,probed
R5703	T11813	T11814	nmod	mAb,203
R5704	T11814	T11812	pobj	203,with
R5705	T11815	T11816	punct	–,217
R5706	T11816	T11814	prep	217,203
R5707	T11817	T11814	acl	followed,203
R5708	T11818	T11817	agent	by,followed
R5709	T11819	T11818	pobj	incubation,by
R5710	T11820	T11819	prep	with,incubation
R5711	T11821	T11822	det	a,antibody
R5712	T11822	T11820	pobj	antibody,with
R5713	T11823	T11824	npadvmod	peroxidase,coupled
R5714	T11824	T11822	amod	coupled,antibody
R5715	T11825	T11822	amod	secondary,antibody
R5716	T11826	T11810	punct	.,probed
R5717	T11828	T11829	nsubj	Detection,was
R5718	T11830	T11829	prep	with,was
R5719	T11831	T11832	amod	enhanced,chemiluminescence
R5720	T11832	T11830	pobj	chemiluminescence,with
R5721	T11833	T11829	punct	.,was
R5724	T12193	T12194	compound	Annexin,A7
R5725	T12194	T12195	compound	A7,immunoreactivity
R5726	T12196	T12195	prep	in,immunoreactivity
R5727	T12197	T12198	amod	early,embryos
R5728	T12198	T12196	pobj	embryos,in
R5729	T12199	T12198	compound	mouse,embryos
R5730	T12200	T12195	punct	.,immunoreactivity
R5731	T12202	T12203	punct	(,A
R5732	T12203	T12204	meta	A,consists
R5733	T12205	T12203	punct	),A
R5734	T12206	T12207	compound	Phase,contrast
R5735	T12207	T12204	dep	contrast,consists
R5736	T12208	T12204	punct	", ",consists
R5737	T12209	T12210	compound	embryo,E5
R5738	T12210	T12204	dep	E5,consists
R5739	T12211	T12204	punct	: ,consists
R5740	T12212	T12213	det	The,cylinder
R5741	T12213	T12204	nsubj	cylinder,consists
R5742	T12214	T12213	compound	egg,cylinder
R5743	T12215	T12204	prep	of,consists
R5744	T12216	T12217	det	an,mass
R5745	T12217	T12215	pobj	mass,of
R5746	T12218	T12217	amod	inner,mass
R5747	T12219	T12217	compound	cell,mass
R5748	T12220	T12221	punct	(,a
R5749	T12221	T12217	parataxis	a,mass
R5750	T12222	T12221	punct	),a
R5751	T12223	T12217	acl	representing,mass
R5752	T12224	T12225	det	the,ectoderm
R5753	T12225	T12223	dobj	ectoderm,representing
R5754	T12226	T12217	cc	and,mass
R5755	T12227	T12228	det	an,layer
R5756	T12228	T12217	conj	layer,mass
R5757	T12229	T12228	amod	outer,layer
R5758	T12230	T12228	prep	of,layer
R5759	T12231	T12232	compound	endoderm,cells
R5760	T12232	T12230	pobj	cells,of
R5761	T12233	T12234	punct	(,b
R5762	T12234	T12228	parataxis	b,layer
R5763	T12235	T12234	punct	),b
R5764	T12236	T12204	punct	.,consists
R5765	T12238	T12239	punct	(,B
R5766	T12239	T12240	meta	B,performed
R5767	T12241	T12239	punct	),B
R5768	T12242	T12240	nsubjpass	Immunostaining,performed
R5769	T12243	T12242	prep	of,Immunostaining
R5770	T12244	T12245	det	the,section
R5771	T12245	T12243	pobj	section,of
R5772	T12246	T12245	compound	paraffin,section
R5773	T12247	T12240	auxpass	was,performed
R5774	T12248	T12249	aux	using,purified
R5775	T12249	T12240	advcl	purified,performed
R5776	T12250	T12251	nmod	mAb,203
R5777	T12251	T12249	dobj	203,purified
R5778	T12252	T12253	punct	–,217
R5779	T12253	T12251	prep	217,203
R5780	T12254	T12251	cc	and,203
R5781	T12255	T12256	npadvmod	Cy3,conjugated
R5782	T12256	T12258	amod	conjugated,IgG
R5783	T12257	T12256	punct	-,conjugated
R5784	T12258	T12251	conj	IgG,203
R5785	T12259	T12258	amod	anti-mouse,IgG
R5786	T12260	T12240	punct	.,performed
R5787	T12262	T12263	compound	Annexin,A7
R5788	T12263	T12264	nsubjpass	A7,expressed
R5789	T12265	T12264	auxpass	is,expressed
R5790	T12266	T12264	prep	in,expressed
R5791	T12267	T12268	det	both,types
R5792	T12268	T12266	pobj	types,in
R5793	T12269	T12268	compound	cell,types
R5794	T12270	T12268	prep	of,types
R5795	T12271	T12272	det	the,cylinder
R5796	T12272	T12270	pobj	cylinder,of
R5797	T12273	T12272	compound	egg,cylinder
R5798	T12274	T12264	prep	with,expressed
R5799	T12275	T12276	det	a,staining
R5800	T12276	T12274	pobj	staining,with
R5801	T12277	T12276	amod	strong,staining
R5802	T12278	T12276	prep	of,staining
R5803	T12279	T12280	det	the,endoderm
R5804	T12280	T12278	pobj	endoderm,of
R5805	T12281	T12276	cc	and,staining
R5806	T12282	T12283	det	a,staining
R5807	T12283	T12276	conj	staining,staining
R5808	T12284	T12283	amod	weaker,staining
R5809	T12285	T12283	prep	of,staining
R5810	T12286	T12287	det	the,ectoderm
R5811	T12287	T12285	pobj	ectoderm,of
R5812	T12288	T12264	punct	.,expressed
R5813	T12290	T12291	det	The,nuclei
R5814	T12291	T12292	nsubj	nuclei,are
R5815	T12293	T12292	acomp	devoid,are
R5816	T12294	T12293	prep	of,devoid
R5817	T12295	T12296	amod	immune,reactions
R5818	T12296	T12294	pobj	reactions,of
R5819	T12297	T12292	punct	.,are
R5820	T12299	T12300	punct	(,control
R5821	T12301	T12300	meta	C,control
R5822	T12302	T12300	punct	),control
R5823	T12303	T12300	amod	Negative,control
R5824	T12304	T12300	acl	using,control
R5825	T12305	T12306	det	the,antibody
R5826	T12306	T12304	dobj	antibody,using
R5827	T12307	T12306	amod	secondary,antibody
R5828	T12308	T12306	compound	Cy3,antibody
R5829	T12309	T12306	punct	-,antibody
R5830	T12310	T12306	advmod	only,antibody
R5831	T12311	T12300	punct	.,control
R5832	T12313	T12314	punct	(,D
R5833	T12314	T12315	meta	D,section
R5834	T12316	T12314	punct	-,D
R5835	T12317	T12314	dep	F,D
R5836	T12318	T12314	punct	),D
R5837	T12319	T12320	compound	Annexin,A7
R5838	T12320	T12321	compound	A7,expression
R5839	T12321	T12315	dep	expression,section
R5840	T12322	T12321	prep	in,expression
R5841	T12323	T12324	det	the,tube
R5842	T12324	T12322	pobj	tube,in
R5843	T12325	T12324	amod	proximal,tube
R5844	T12326	T12324	amod	neural,tube
R5845	T12327	T12328	punct	(,D
R5846	T12328	T12324	parataxis	D,tube
R5847	T12329	T12328	punct	),D
R5848	T12330	T12324	cc	and,tube
R5849	T12331	T12332	amod	nearby,fold
R5850	T12332	T12324	conj	fold,tube
R5851	T12333	T12332	amod	neural,fold
R5852	T12334	T12335	punct	(,F
R5853	T12335	T12332	parataxis	F,fold
R5854	T12336	T12335	nmod	E,F
R5855	T12337	T12335	punct	",",F
R5856	T12338	T12335	punct	),F
R5857	T12339	T12315	punct	", ",section
R5858	T12340	T12341	compound	embryo,E8
R5859	T12341	T12315	dep	E8,section
R5860	T12342	T12315	punct	", ",section
R5861	T12343	T12315	amod	transverse,section
R5862	T12344	T12315	punct	.,section
R5863	T12346	T12347	nsubjpass	Immunolabeling,performed
R5864	T12348	T12346	prep	of,Immunolabeling
R5865	T12349	T12350	compound	Annexin,A7
R5866	T12350	T12348	pobj	A7,of
R5867	T12351	T12347	auxpass	was,performed
R5868	T12352	T12347	prep	with,performed
R5869	T12353	T12354	amod	purified,203
R5870	T12354	T12352	pobj	203,with
R5871	T12355	T12354	nmod	mAb,203
R5872	T12356	T12357	punct	–,217
R5873	T12357	T12354	prep	217,203
R5874	T12358	T12347	cc	and,performed
R5875	T12359	T12360	nsubj	visualization,was
R5876	T12360	T12347	conj	was,performed
R5877	T12361	T12360	prep	with,was
R5878	T12362	T12363	det	an,IgG
R5879	T12363	T12361	pobj	IgG,with
R5880	T12364	T12365	compound	Alexa,Fluor
R5881	T12365	T12366	npadvmod	Fluor,conjugated
R5882	T12366	T12363	amod	conjugated,IgG
R5883	T12367	T12365	nummod	488,Fluor
R5884	T12368	T12366	punct	-,conjugated
R5885	T12369	T12363	amod	anti-mouse,IgG
R5886	T12370	T12360	punct	.,was
R5887	T12372	T12373	punct	(,D
R5888	T12373	T12374	meta	D,is
R5889	T12375	T12373	punct	),D
R5890	T12376	T12377	det	An,immunostaining
R5891	T12377	T12374	nsubj	immunostaining,is
R5892	T12378	T12377	amod	intense,immunostaining
R5893	T12379	T12380	compound	Annexin,A7
R5894	T12380	T12377	compound	A7,immunostaining
R5895	T12381	T12374	acomp	detectable,is
R5896	T12382	T12374	prep	in,is
R5897	T12383	T12384	det	the,neuroepithelium
R5898	T12384	T12382	pobj	neuroepithelium,in
R5899	T12385	T12384	prep	of,neuroepithelium
R5900	T12386	T12387	det	the,tube
R5901	T12387	T12385	pobj	tube,of
R5902	T12388	T12387	amod	neural,tube
R5903	T12389	T12390	punct	(,lumen
R5904	T12390	T12374	parataxis	lumen,is
R5905	T12391	T12390	dep	a,lumen
R5906	T12392	T12390	punct	", ",lumen
R5907	T12393	T12390	prep	of,lumen
R5908	T12394	T12395	amod	neural,tube
R5909	T12395	T12393	pobj	tube,of
R5910	T12396	T12390	punct	),lumen
R5911	T12397	T12374	punct	.,is
R5912	T12399	T12400	punct	(,E
R5913	T12400	T12401	meta	E,show
R5914	T12402	T12400	punct	",",E
R5915	T12403	T12400	dep	F,E
R5916	T12404	T12400	punct	),E
R5917	T12405	T12406	amod	Higher,magnifications
R5918	T12406	T12401	nsubj	magnifications,show
R5919	T12407	T12406	prep	of,magnifications
R5920	T12408	T12409	det	the,neuroepithelium
R5921	T12409	T12407	pobj	neuroepithelium,of
R5922	T12410	T12411	mark	that,expressed
R5923	T12411	T12401	ccomp	expressed,show
R5924	T12412	T12413	compound	Annexin,A7
R5925	T12413	T12411	nsubjpass	A7,expressed
R5926	T12414	T12411	auxpass	is,expressed
R5927	T12415	T12411	prep	in,expressed
R5928	T12416	T12417	det	the,cytosol
R5929	T12417	T12415	pobj	cytosol,in
R5930	T12418	T12401	punct	.,show
R5931	T12420	T12421	nsubj	Arrowheads,point
R5932	T12422	T12421	prep	to,point
R5933	T12423	T12424	compound	Annexin,A7
R5934	T12424	T12425	compound	A7,immunoreactivity
R5935	T12425	T12422	pobj	immunoreactivity,to
R5936	T12426	T12421	prep	in,point
R5937	T12427	T12428	det	the,cytosol
R5938	T12428	T12426	pobj	cytosol,in
R5939	T12429	T12421	punct	.,point
R5940	T12431	T12432	punct	(,tube
R5941	T12433	T12432	meta	G,tube
R5942	T12434	T12432	punct	),tube
R5943	T12435	T12436	compound	Phase,contrast
R5944	T12436	T12432	dep	contrast,tube
R5945	T12437	T12432	punct	", ",tube
R5946	T12438	T12439	compound	embryo,E13
R5947	T12439	T12432	dep	E13,tube
R5948	T12440	T12432	punct	", ",tube
R5949	T12441	T12432	amod	caudal,tube
R5950	T12442	T12432	amod	neural,tube
R5951	T12443	T12432	punct	.,tube
R5952	T12445	T12446	punct	(,H
R5953	T12446	T12447	meta	H,performed
R5954	T12448	T12446	punct	),H
R5955	T12449	T12447	nsubjpass	Immunostaining,performed
R5956	T12450	T12449	prep	of,Immunostaining
R5957	T12451	T12452	det	the,section
R5958	T12452	T12450	pobj	section,of
R5959	T12453	T12452	compound	paraffin,section
R5960	T12454	T12447	auxpass	was,performed
R5961	T12455	T12447	advcl	using,performed
R5962	T12456	T12457	amod	purified,203
R5963	T12457	T12455	dobj	203,using
R5964	T12458	T12457	nmod	mAb,203
R5965	T12459	T12460	punct	–,217
R5966	T12460	T12457	prep	217,203
R5967	T12461	T12457	cc	and,203
R5968	T12462	T12463	compound	Alexa,Fluor
R5969	T12463	T12464	npadvmod	Fluor,conjugated
R5970	T12464	T12467	amod	conjugated,IgG
R5971	T12465	T12463	nummod	488,Fluor
R5972	T12466	T12464	punct	-,conjugated
R5973	T12467	T12457	conj	IgG,203
R5974	T12468	T12467	amod	anti-mouse,IgG
R5975	T12469	T12447	punct	.,performed
R5976	T12471	T12472	punct	(,I
R5977	T12472	T12473	meta	I,control
R5978	T12474	T12472	punct	),I
R5979	T12475	T12473	amod	Negative,control
R5980	T12476	T12473	acl	using,control
R5981	T12477	T12478	det	the,antibody
R5982	T12478	T12476	dobj	antibody,using
R5983	T12479	T12478	amod	secondary,antibody
R5984	T12480	T12481	nmod	Alexa,Fluor
R5985	T12481	T12478	nmod	Fluor,antibody
R5986	T12482	T12481	nummod	488,Fluor
R5987	T12483	T12478	punct	-,antibody
R5988	T12484	T12478	advmod	only,antibody
R5989	T12485	T12473	punct	.,control
R5990	T12488	T12487	punct	", ",Bar
R5991	T12489	T12490	nummod	20,μm
R5992	T12490	T12487	appos	μm,Bar
R5993	T12491	T12487	punct	.,Bar
R5996	T12790	T12791	amod	Subcellular,localization
R5997	T12792	T12791	prep	of,localization
R5998	T12793	T12794	compound	Annexin,A7
R5999	T12794	T12792	pobj	A7,of
R6000	T12795	T12791	prep	in,localization
R6001	T12796	T12797	compound	embryos,E13
R6002	T12797	T12795	pobj	E13,in
R6003	T12798	T12797	punct	", ",E13
R6004	T12799	T12797	conj	E15,E13
R6005	T12800	T12799	cc	and,E15
R6006	T12801	T12799	conj	E16,E15
R6007	T12802	T12791	punct	.,localization
R6008	T12804	T12805	compound	Paraffin,sections
R6009	T12805	T12806	nsubjpass	sections,stained
R6010	T12807	T12805	prep	of,sections
R6011	T12808	T12809	amod	embryonic,brain
R6012	T12809	T12807	pobj	brain,of
R6013	T12810	T12806	auxpass	were,stained
R6014	T12811	T12806	prep	with,stained
R6015	T12812	T12813	amod	purified,203
R6016	T12813	T12811	pobj	203,with
R6017	T12814	T12813	nmod	mAb,203
R6018	T12815	T12816	punct	–,217
R6019	T12816	T12813	prep	217,203
R6020	T12817	T12806	punct	.,stained
R6021	T12819	T12820	compound	Annexin,A7
R6022	T12820	T12821	nsubjpass	A7,visualized
R6023	T12822	T12821	auxpass	was,visualized
R6024	T12823	T12821	prep	with,visualized
R6025	T12824	T12825	compound	Alexa,Fluor
R6026	T12825	T12826	npadvmod	Fluor,conjugated
R6027	T12826	T12829	amod	conjugated,antibody
R6028	T12827	T12825	nummod	488,Fluor
R6029	T12828	T12826	punct	-,conjugated
R6030	T12829	T12823	pobj	antibody,with
R6031	T12830	T12829	amod	secondary,antibody
R6032	T12831	T12821	punct	.,visualized
R6033	T12833	T12834	punct	(,A
R6034	T12834	T12835	meta	A,given
R6035	T12836	T12834	punct	),A
R6036	T12837	T12838	dep	Overview,stained
R6037	T12838	T12835	dep	stained,given
R6038	T12839	T12838	punct	", ",stained
R6039	T12840	T12838	prep	at,stained
R6040	T12841	T12840	pobj	E16,at
R6041	T12842	T12843	det	the,cells
R6042	T12843	T12838	nsubjpass	cells,stained
R6043	T12844	T12843	amod	immature,cells
R6044	T12845	T12846	npadvmod	GFAP,negative
R6045	T12846	T12843	amod	negative,cells
R6046	T12847	T12846	punct	-,negative
R6047	T12848	T12843	prep	of,cells
R6048	T12849	T12850	det	the,neocortex
R6049	T12850	T12848	pobj	neocortex,of
R6050	T12851	T12850	amod	forming,neocortex
R6051	T12852	T12850	amod	cerebral,neocortex
R6052	T12853	T12854	punct	(,b
R6053	T12854	T12843	parataxis	b,cells
R6054	T12855	T12854	punct	),b
R6055	T12856	T12843	acl	surrounding,cells
R6056	T12857	T12858	det	the,ventricle
R6057	T12858	T12856	dobj	ventricle,surrounding
R6058	T12859	T12858	amod	lateral,ventricle
R6059	T12860	T12861	punct	(,a
R6060	T12861	T12858	parataxis	a,ventricle
R6061	T12862	T12861	punct	),a
R6062	T12863	T12838	auxpass	are,stained
R6063	T12864	T12838	advmod	strongly,stained
R6064	T12865	T12835	punct	;,given
R6065	T12866	T12835	dep	square,given
R6066	T12867	T12835	punct	", ",given
R6067	T12868	T12869	det	a,magnification
R6068	T12869	T12835	nsubjpass	magnification,given
R6069	T12870	T12869	amod	higher,magnification
R6070	T12871	T12869	prep	of,magnification
R6071	T12872	T12873	det	this,area
R6072	T12873	T12871	pobj	area,of
R6073	T12874	T12835	auxpass	is,given
R6074	T12875	T12835	prep	in,given
R6075	T12876	T12875	punct	(,in
R6076	T12877	T12875	pobj	I,in
R6077	T12878	T12835	punct	),given
R6078	T12879	T12835	punct	.,given
R6079	T12881	T12882	punct	(,B
R6080	T12882	T12883	meta	B,shows
R6081	T12884	T12882	punct	),B
R6082	T12885	T12886	amod	Higher,magnification
R6083	T12886	T12883	nsubj	magnification,shows
R6084	T12887	T12886	prep	of,magnification
R6085	T12888	T12889	det	the,E13
R6086	T12889	T12887	pobj	E13,of
R6087	T12890	T12889	amod	earlier,E13
R6088	T12891	T12889	compound	stage,E13
R6089	T12892	T12893	punct	(,H
R6090	T12893	T12886	parataxis	H,magnification
R6091	T12894	T12893	punct	),H
R6092	T12895	T12883	punct	", ",shows
R6093	T12896	T12897	mark	that,stained
R6094	T12897	T12883	ccomp	stained,shows
R6095	T12898	T12899	det	the,cells
R6096	T12899	T12897	nsubjpass	cells,stained
R6097	T12900	T12897	auxpass	are,stained
R6098	T12901	T12897	prep	in,stained
R6099	T12902	T12903	det	the,cytosol
R6100	T12903	T12901	pobj	cytosol,in
R6101	T12904	T12905	punct	(,arrowhead
R6102	T12905	T12897	parataxis	arrowhead,stained
R6103	T12906	T12905	punct	),arrowhead
R6104	T12907	T12883	punct	.,shows
R6105	T12909	T12910	punct	(,C
R6106	T12910	T12911	meta	C,rounded
R6107	T12912	T12910	punct	),C
R6108	T12913	T12914	nummod	Two,days
R6109	T12914	T12915	npadvmod	days,later
R6110	T12915	T12911	advmod	later,rounded
R6111	T12916	T12911	prep	at,rounded
R6112	T12917	T12916	pobj	E15,at
R6113	T12918	T12919	det	the,cells
R6114	T12919	T12911	nsubj	cells,rounded
R6115	T12920	T12911	aux	have,rounded
R6116	T12921	T12911	prt	up,rounded
R6117	T12922	T12911	punct	", ",rounded
R6118	T12923	T12911	cc	and,rounded
R6119	T12924	T12925	compound	Annexin,A7
R6120	T12925	T12926	nsubj	A7,stays
R6121	T12926	T12911	conj	stays,rounded
R6122	T12927	T12926	prep	in,stays
R6123	T12928	T12929	det	the,cytosol
R6124	T12929	T12927	pobj	cytosol,in
R6125	T12930	T12911	punct	.,rounded
R6126	T12932	T12933	punct	(,D
R6127	T12933	T12934	meta	D,becomes
R6128	T12935	T12933	punct	),D
R6129	T12936	T12934	prep	At,becomes
R6130	T12937	T12936	pobj	E16,At
R6131	T12938	T12939	det	the,staining
R6132	T12939	T12934	nsubj	staining,becomes
R6133	T12940	T12939	amod	first,staining
R6134	T12941	T12939	amod	nuclear,staining
R6135	T12942	T12934	acomp	apparent,becomes
R6136	T12943	T12944	punct	(,arrowhead
R6137	T12944	T12934	parataxis	arrowhead,becomes
R6138	T12945	T12944	punct	),arrowhead
R6139	T12946	T12934	prep	in,becomes
R6140	T12947	T12946	pobj	cells,in
R6141	T12948	T12947	prep	of,cells
R6142	T12949	T12950	det	the,zone
R6143	T12950	T12948	pobj	zone,of
R6144	T12951	T12950	amod	intermediate,zone
R6145	T12952	T12950	acl	located,zone
R6146	T12953	T12952	prep	between,located
R6147	T12954	T12955	amod	ventricular,zone
R6148	T12955	T12953	pobj	zone,between
R6149	T12956	T12955	amod	germinative,zone
R6150	T12957	T12955	cc	and,zone
R6151	T12958	T12959	amod	marginal,cortex
R6152	T12959	T12955	conj	cortex,zone
R6153	T12960	T12959	amod	neopallial,cortex
R6154	T12961	T12962	mark	as,seen
R6155	T12962	T12934	advcl	seen,becomes
R6156	T12963	T12962	prep	in,seen
R6157	T12964	T12963	punct	(,in
R6158	T12965	T12963	pobj	I,in
R6159	T12966	T12963	punct	),in
R6160	T12967	T12968	punct	", ",oval
R6161	T12968	T12963	parataxis	oval,in
R6162	T12969	T12934	punct	.,becomes
R6163	T12971	T12972	punct	(,E
R6164	T12972	T12973	meta	E,confirms
R6165	T12974	T12972	punct	-,E
R6166	T12975	T12972	dep	G,E
R6167	T12976	T12972	punct	),E
R6168	T12977	T12978	amod	Confocal,microscopy
R6169	T12978	T12973	nsubj	microscopy,confirms
R6170	T12979	T12980	det	the,results
R6171	T12980	T12973	dobj	results,confirms
R6172	T12981	T12980	prep	in,results
R6173	T12982	T12981	pobj	B,in
R6174	T12983	T12984	punct	-,D
R6175	T12984	T12982	prep	D,B
R6176	T12985	T12973	punct	.,confirms
R6177	T12987	T12988	punct	(,H
R6178	T12988	T12989	meta	H,stained
R6179	T12990	T12988	punct	),H
R6180	T12991	T12989	dep	Overview,stained
R6181	T12992	T12989	punct	", ",stained
R6182	T12993	T12989	prep	at,stained
R6183	T12994	T12993	pobj	E13,at
R6184	T12995	T12996	det	the,cells
R6185	T12996	T12989	nsubjpass	cells,stained
R6186	T12997	T12996	amod	immature,cells
R6187	T12998	T12999	npadvmod	GFAP,negative
R6188	T12999	T12996	amod	negative,cells
R6189	T13000	T12999	punct	-,negative
R6190	T13001	T12989	auxpass	are,stained
R6191	T13002	T12989	advmod	strongly,stained
R6192	T13003	T13004	punct	;,ventricle
R6193	T13004	T12989	parataxis	ventricle,stained
R6194	T13005	T13004	dep	a,ventricle
R6195	T13006	T13004	punct	", ",ventricle
R6196	T13007	T13004	amod	lateral,ventricle
R6197	T13008	T12989	punct	.,stained
R6198	T13010	T13011	punct	(,I
R6199	T13011	T13012	meta	I,magnification
R6200	T13013	T13011	punct	),I
R6201	T13014	T13012	amod	Higher,magnification
R6202	T13015	T13012	prep	of,magnification
R6203	T13016	T13017	det	a,section
R6204	T13017	T13015	pobj	section,of
R6205	T13018	T13017	amod	cortical,section
R6206	T13019	T13017	prep	of,section
R6207	T13020	T13019	punct	(,of
R6208	T13021	T13019	pobj	A,of
R6209	T13022	T13023	punct	", ",square
R6210	T13023	T13021	parataxis	square,A
R6211	T13024	T13023	punct	),square
R6212	T13025	T13012	acl	demonstrating,magnification
R6213	T13026	T13027	amod	ventricular,zones
R6214	T13027	T13025	dobj	zones,demonstrating
R6215	T13028	T13026	punct	", ",ventricular
R6216	T13029	T13026	conj	intermediate,ventricular
R6217	T13030	T13029	punct	", ",intermediate
R6218	T13031	T13029	cc	and,intermediate
R6219	T13032	T13029	conj	marginal,intermediate
R6220	T13033	T13012	punct	;,magnification
R6221	T13034	T13035	dep	oval,given
R6222	T13035	T13012	dep	given,magnification
R6223	T13036	T13035	punct	", ",given
R6224	T13037	T13038	det	a,magnification
R6225	T13038	T13035	nsubjpass	magnification,given
R6226	T13039	T13038	amod	higher,magnification
R6227	T13040	T13038	prep	of,magnification
R6228	T13041	T13042	det	this,area
R6229	T13042	T13040	pobj	area,of
R6230	T13043	T13035	auxpass	is,given
R6231	T13044	T13035	prep	in,given
R6232	T13045	T13044	punct	(,in
R6233	T13046	T13047	nmod	D,G
R6234	T13047	T13044	pobj	G,in
R6235	T13048	T13047	punct	",",G
R6236	T13049	T13012	punct	),magnification
R6237	T13050	T13012	punct	.,magnification
R6244	T13536	T13537	compound	Annexin,A7
R6245	T13537	T13538	nsubj	A7,is
R6246	T13539	T13538	acomp	present,is
R6247	T13540	T13538	prep	in,is
R6248	T13541	T13540	pobj	neurons,in
R6249	T13542	T13541	cc	and,neurons
R6250	T13543	T13541	conj	astrocytes,neurons
R6251	T13544	T13541	prep	of,neurons
R6252	T13545	T13546	det	the,temporalis
R6253	T13546	T13544	pobj	temporalis,of
R6254	T13547	T13546	compound	cortex,temporalis
R6255	T13548	T13546	cc	and,temporalis
R6256	T13549	T13550	amod	hippocampal,formation
R6257	T13550	T13546	conj	formation,temporalis
R6258	T13551	T13546	prep	of,temporalis
R6259	T13552	T13553	nummod	10,weeks
R6260	T13553	T13555	npadvmod	weeks,old
R6261	T13554	T13553	punct	-,weeks
R6262	T13555	T13557	amod	old,mice
R6263	T13556	T13555	punct	-,old
R6264	T13557	T13551	pobj	mice,of
R6265	T13558	T13538	punct	.,is
R6266	T13560	T13561	punct	(,A
R6267	T13561	T13562	meta	A,presents
R6268	T13563	T13561	punct	),A
R6269	T13564	T13565	amod	Low,magnification
R6270	T13565	T13562	nsubj	magnification,presents
R6271	T13566	T13565	prep	of,magnification
R6272	T13567	T13568	det	the,temporalis
R6273	T13568	T13566	pobj	temporalis,of
R6274	T13569	T13568	compound	cortex,temporalis
R6275	T13570	T13571	det	an,expression
R6276	T13571	T13562	dobj	expression,presents
R6277	T13572	T13573	compound	Annexin,A7
R6278	T13573	T13571	compound	A7,expression
R6279	T13574	T13571	prep	in,expression
R6280	T13575	T13574	pobj	cells,in
R6281	T13576	T13575	prep	of,cells
R6282	T13577	T13578	det	the,border
R6283	T13578	T13576	pobj	border,of
R6284	T13579	T13578	amod	pial,border
R6285	T13580	T13571	punct	", ",expression
R6286	T13581	T13571	prep	in,expression
R6287	T13582	T13581	pobj	neurons,in
R6288	T13583	T13582	prep	of,neurons
R6289	T13584	T13585	det	all,laminae
R6290	T13585	T13583	pobj	laminae,of
R6291	T13586	T13585	nummod	six,laminae
R6292	T13587	T13585	amod	isocortical,laminae
R6293	T13588	T13571	punct	", ",expression
R6294	T13589	T13571	cc	and,expression
R6295	T13590	T13591	det	a,signal
R6296	T13591	T13571	conj	signal,expression
R6297	T13592	T13591	amod	weak,signal
R6298	T13593	T13591	prep	in,signal
R6299	T13594	T13595	det	the,matter
R6300	T13595	T13593	pobj	matter,in
R6301	T13596	T13595	amod	adjacent,matter
R6302	T13597	T13595	amod	white,matter
R6303	T13598	T13562	punct	.,presents
R6304	T13600	T13601	punct	(,B
R6305	T13601	T13602	meta	B,section
R6306	T13603	T13601	punct	),B
R6307	T13604	T13602	amod	Corresponding,section
R6308	T13605	T13602	acl	stained,section
R6309	T13606	T13605	prep	with,stained
R6310	T13607	T13606	pobj	GFAP,with
R6311	T13608	T13602	punct	.,section
R6312	T13610	T13611	punct	(,C
R6313	T13611	T13612	meta	C,Staining
R6314	T13613	T13611	punct	),C
R6315	T13614	T13612	prep	in,Staining
R6316	T13615	T13616	det	the,pyramidalis
R6317	T13616	T13614	pobj	pyramidalis,in
R6318	T13617	T13616	compound	Stratum,pyramidalis
R6319	T13618	T13619	punct	(,a
R6320	T13619	T13616	parataxis	a,pyramidalis
R6321	T13620	T13619	punct	),a
R6322	T13621	T13614	cc	and,in
R6323	T13622	T13614	conj	in,in
R6324	T13623	T13624	det	the,gyrus
R6325	T13624	T13622	pobj	gyrus,in
R6326	T13625	T13624	compound	dentate,gyrus
R6327	T13626	T13624	prep	of,gyrus
R6328	T13627	T13628	det	the,hippocampus
R6329	T13628	T13626	pobj	hippocampus,of
R6330	T13629	T13612	punct	;,Staining
R6331	T13630	T13631	dep	square,given
R6332	T13631	T13612	dep	given,Staining
R6333	T13632	T13631	punct	", ",given
R6334	T13633	T13634	det	a,magnification
R6335	T13634	T13631	nsubjpass	magnification,given
R6336	T13635	T13634	amod	higher,magnification
R6337	T13636	T13634	prep	of,magnification
R6338	T13637	T13638	amod	acorresponding,area
R6339	T13638	T13636	pobj	area,of
R6340	T13639	T13631	auxpass	is,given
R6341	T13640	T13631	prep	in,given
R6342	T13641	T13640	punct	(,in
R6343	T13642	T13643	nmod	G,H
R6344	T13643	T13640	pobj	H,in
R6345	T13644	T13643	punct	",",H
R6346	T13645	T13640	punct	),in
R6347	T13646	T13612	punct	.,Staining
R6348	T13648	T13649	det	An,immunostaining
R6349	T13649	T13653	nsubj	immunostaining,is
R6350	T13650	T13649	amod	intense,immunostaining
R6351	T13651	T13652	compound	Annexin,A7
R6352	T13652	T13649	compound	A7,immunostaining
R6353	T13654	T13653	acomp	detectable,is
R6354	T13655	T13653	punct	.,is
R6355	T13657	T13658	punct	(,D
R6356	T13658	T13659	meta	D,section
R6357	T13660	T13658	punct	),D
R6358	T13661	T13659	amod	Corresponding,section
R6359	T13662	T13659	acl	stained,section
R6360	T13663	T13662	prep	with,stained
R6361	T13664	T13665	amod	secondary,antibody
R6362	T13665	T13663	pobj	antibody,with
R6363	T13666	T13665	advmod	only,antibody
R6364	T13667	T13659	punct	.,section
R6365	T13669	T13670	punct	(,E
R6366	T13670	T13671	meta	E,Presence
R6367	T13672	T13670	punct	),E
R6368	T13673	T13671	prep	of,Presence
R6369	T13674	T13675	compound	Annexin,A7
R6370	T13675	T13673	pobj	A7,of
R6371	T13676	T13671	prep	in,Presence
R6372	T13677	T13678	amod	pyramidal,neurons
R6373	T13678	T13676	pobj	neurons,in
R6374	T13679	T13680	punct	(,externa
R6375	T13680	T13678	parataxis	externa,neurons
R6376	T13681	T13680	compound	lamina,externa
R6377	T13682	T13680	compound	pyramidalis,externa
R6378	T13683	T13680	punct	),externa
R6379	T13684	T13678	prep	of,neurons
R6380	T13685	T13686	det	the,temporalis
R6381	T13686	T13684	pobj	temporalis,of
R6382	T13687	T13686	compound	isocortex,temporalis
R6383	T13688	T13671	punct	.,Presence
R6384	T13690	T13691	det	These,neurons
R6385	T13691	T13692	nsubjpass	neurons,identified
R6386	T13693	T13692	auxpass	were,identified
R6387	T13694	T13692	prep	based,identified
R6388	T13695	T13694	prep	on,based
R6389	T13696	T13697	poss	their,morphology
R6390	T13697	T13695	pobj	morphology,on
R6391	T13698	T13697	punct	", ",morphology
R6392	T13699	T13697	conj	distribution,morphology
R6393	T13700	T13699	cc	and,distribution
R6394	T13701	T13699	conj	lack,distribution
R6395	T13702	T13701	prep	of,lack
R6396	T13703	T13704	compound	GFAP,staining
R6397	T13704	T13702	pobj	staining,of
R6398	T13705	T13692	punct	.,identified
R6399	T13707	T13708	nsubj	AnnexinA7,exhibits
R6400	T13709	T13710	det	a,staining
R6401	T13710	T13708	dobj	staining,exhibits
R6402	T13711	T13710	compound	punctate,staining
R6403	T13712	T13710	punct	", ",staining
R6404	T13713	T13714	dep	which,is
R6405	T13714	T13710	relcl	is,staining
R6406	T13715	T13714	acomp	pronounced,is
R6407	T13716	T13714	prep	in,is
R6408	T13717	T13718	det	the,nucleus
R6409	T13718	T13716	pobj	nucleus,in
R6410	T13719	T13720	punct	(,arrowhead
R6411	T13720	T13708	parataxis	arrowhead,exhibits
R6412	T13721	T13720	punct	),arrowhead
R6413	T13722	T13708	punct	.,exhibits
R6414	T13724	T13725	punct	(,F
R6415	T13725	T13726	meta	F,shows
R6416	T13727	T13725	punct	),F
R6417	T13728	T13729	amod	Higher,magnification
R6418	T13729	T13726	nsubj	magnification,shows
R6419	T13730	T13729	prep	of,magnification
R6420	T13731	T13730	pobj	image,of
R6421	T13732	T13733	punct	(,A
R6422	T13733	T13729	parataxis	A,magnification
R6423	T13734	T13733	punct	),A
R6424	T13735	T13726	advmod	also,shows
R6425	T13736	T13737	compound	Annexin,A7
R6426	T13737	T13726	dobj	A7,shows
R6427	T13738	T13726	prep	in,shows
R6428	T13739	T13738	pobj	nuclei,in
R6429	T13740	T13739	prep	of,nuclei
R6430	T13741	T13740	pobj	neurons,of
R6431	T13742	T13743	punct	(,arrowhead
R6432	T13743	T13741	parataxis	arrowhead,neurons
R6433	T13744	T13745	compound	lamina,externa
R6434	T13745	T13743	dep	externa,arrowhead
R6435	T13746	T13745	compound	granularis,externa
R6436	T13747	T13748	punct	(,corpuscularis
R6437	T13748	T13745	parataxis	corpuscularis,externa
R6438	T13749	T13748	punct	),corpuscularis
R6439	T13750	T13743	punct	", ",arrowhead
R6440	T13751	T13743	punct	),arrowhead
R6441	T13752	T13738	cc	and,in
R6442	T13753	T13738	conj	in,in
R6443	T13754	T13755	det	the,cytoplasm
R6444	T13755	T13753	pobj	cytoplasm,in
R6445	T13756	T13755	cc	and,cytoplasm
R6446	T13757	T13755	conj	nuclei,cytoplasm
R6447	T13758	T13755	prep	of,cytoplasm
R6448	T13759	T13758	pobj	astrocytes,of
R6449	T13760	T13761	punct	(,arrow
R6450	T13761	T13759	parataxis	arrow,astrocytes
R6451	T13762	T13763	compound	lamina,molecularis
R6452	T13763	T13761	dep	molecularis,arrow
R6453	T13764	T13761	punct	", ",arrow
R6454	T13765	T13761	punct	;,arrow
R6455	T13766	T13767	npadvmod	GFAP,confirmed
R6456	T13767	T13761	amod	confirmed,arrow
R6457	T13768	T13767	punct	-,confirmed
R6458	T13769	T13761	punct	),arrow
R6459	T13770	T13726	punct	.,shows
R6460	T13772	T13773	punct	(,G
R6461	T13773	T13774	meta	G,confirms
R6462	T13775	T13773	punct	),G
R6463	T13776	T13777	amod	Higher,magnification
R6464	T13777	T13774	nsubj	magnification,confirms
R6465	T13778	T13777	prep	of,magnification
R6466	T13779	T13780	det	the,neurons
R6467	T13780	T13778	pobj	neurons,of
R6468	T13781	T13780	amod	pyramidal,neurons
R6469	T13782	T13780	prep	in,neurons
R6470	T13783	T13784	det	the,hippocampus
R6471	T13784	T13782	pobj	hippocampus,in
R6472	T13785	T13786	det	the,presence
R6473	T13786	T13774	dobj	presence,confirms
R6474	T13787	T13786	prep	of,presence
R6475	T13788	T13789	det	the,protein
R6476	T13789	T13787	pobj	protein,of
R6477	T13790	T13791	compound	Annexin,A7
R6478	T13791	T13789	compound	A7,protein
R6479	T13792	T13786	prep	in,presence
R6480	T13793	T13794	det	the,nucleus
R6481	T13794	T13792	pobj	nucleus,in
R6482	T13795	T13796	punct	(,arrowhead
R6483	T13796	T13794	parataxis	arrowhead,nucleus
R6484	T13797	T13796	punct	),arrowhead
R6485	T13798	T13794	prep	of,nucleus
R6486	T13799	T13800	amod	mature,neurons
R6487	T13800	T13798	pobj	neurons,of
R6488	T13801	T13774	punct	.,confirms
R6489	T13803	T13804	punct	(,H
R6490	T13804	T13805	meta	H,stained
R6491	T13806	T13804	punct	),H
R6492	T13807	T13808	aux	To,confirm
R6493	T13808	T13805	advcl	confirm,stained
R6494	T13809	T13808	advmod	further,confirm
R6495	T13810	T13808	dobj	this,confirm
R6496	T13811	T13805	punct	", ",stained
R6497	T13812	T13813	det	a,section
R6498	T13813	T13805	nsubjpass	section,stained
R6499	T13814	T13813	amod	similar,section
R6500	T13815	T13813	acl	derived,section
R6501	T13816	T13815	prep	from,derived
R6502	T13817	T13818	det	an,mouse
R6503	T13818	T13816	pobj	mouse,from
R6504	T13819	T13818	nmod	AnxA7,mouse
R6505	T13820	T13819	punct	-,AnxA7
R6506	T13821	T13819	punct	/,AnxA7
R6507	T13822	T13819	punct	-,AnxA7
R6508	T13823	T13805	auxpass	was,stained
R6509	T13824	T13805	prep	with,stained
R6510	T13825	T13826	det	the,antibody
R6511	T13826	T13824	pobj	antibody,with
R6512	T13827	T13828	npadvmod	annexin,specific
R6513	T13828	T13826	amod	specific,antibody
R6514	T13829	T13805	cc	and,stained
R6515	T13830	T13805	conj	lacked,stained
R6516	T13831	T13832	det	the,signal
R6517	T13832	T13830	dobj	signal,lacked
R6518	T13833	T13832	amod	nuclear,signal
R6519	T13834	T13805	punct	.,stained
R6520	T13836	T13837	det	The,stain
R6521	T13837	T13839	nsubj	stain,is
R6522	T13838	T13837	amod	residual,stain
R6523	T13840	T13837	prep	of,stain
R6524	T13841	T13842	det	the,tissue
R6525	T13842	T13840	pobj	tissue,of
R6526	T13843	T13839	acomp	unspecific,is
R6527	T13844	T13839	punct	", ",is
R6528	T13845	T13846	mark	as,observed
R6529	T13846	T13839	advcl	observed,is
R6530	T13847	T13846	nsubjpass	it,observed
R6531	T13848	T13846	auxpass	is,observed
R6532	T13849	T13846	advmod	also,observed
R6533	T13850	T13846	prep	in,observed
R6534	T13851	T13850	pobj	controls,in
R6535	T13852	T13851	prep	of,controls
R6536	T13853	T13854	det	the,brain
R6537	T13854	T13852	pobj	brain,of
R6538	T13855	T13854	nmod	AnxA7,brain
R6539	T13856	T13855	punct	-,AnxA7
R6540	T13857	T13855	punct	/,AnxA7
R6541	T13858	T13855	punct	-,AnxA7
R6542	T13859	T13846	advcl	omitting,observed
R6543	T13860	T13861	det	the,antibody
R6544	T13861	T13859	dobj	antibody,omitting
R6545	T13862	T13861	amod	primary,antibody
R6546	T13863	T13864	punct	(,shown
R6547	T13864	T13839	parataxis	shown,is
R6548	T13865	T13864	nsubj	data,shown
R6549	T13866	T13864	neg	not,shown
R6550	T13867	T13864	punct	),shown
R6551	T13868	T13839	punct	.,is
R6552	T13870	T13871	det	All,sections
R6553	T13871	T13873	nsubjpass	sections,stained
R6554	T13872	T13871	compound	paraffin,sections
R6555	T13874	T13873	auxpass	were,stained
R6556	T13875	T13873	prep	with,stained
R6557	T13876	T13877	nmod	mAb,203
R6558	T13877	T13875	pobj	203,with
R6559	T13878	T13879	punct	–,217
R6560	T13879	T13877	prep	217,203
R6561	T13880	T13881	punct	(,G
R6562	T13881	T13877	parataxis	G,203
R6563	T13882	T13881	nmod	A,G
R6564	T13883	T13881	punct	", ",G
R6565	T13884	T13881	nmod	C,G
R6566	T13885	T13881	punct	", ",G
R6567	T13886	T13881	nmod	E,G
R6568	T13887	T13881	punct	", ",G
R6569	T13888	T13881	nmod	F,G
R6570	T13889	T13881	punct	", ",G
R6571	T13890	T13881	punct	),G
R6572	T13891	T13877	cc	or,203
R6573	T13892	T13893	amod	anti-GFAP,antibody
R6574	T13893	T13877	conj	antibody,203
R6575	T13894	T13893	punct	-,antibody
R6576	T13895	T13896	punct	(,B
R6577	T13896	T13893	parataxis	B,antibody
R6578	T13897	T13896	punct	),B
R6579	T13898	T13873	punct	.,stained
R6580	T13900	T13901	det	The,section
R6581	T13901	T13904	nsubj	section,lacks
R6582	T13902	T13901	amod	hippocampal,section
R6583	T13903	T13901	compound	control,section
R6584	T13905	T13906	punct	(,D
R6585	T13906	T13901	parataxis	D,section
R6586	T13907	T13906	punct	),D
R6587	T13908	T13909	det	the,antibody
R6588	T13909	T13904	dobj	antibody,lacks
R6589	T13910	T13909	amod	primary,antibody
R6590	T13911	T13904	punct	.,lacks
R6597	T14188	T14189	compound	Annexin,A7
R6598	T14189	T14190	compound	A7,immunostaining
R6599	T14191	T14190	prep	in,immunostaining
R6600	T14192	T14193	det	the,cerebellum
R6601	T14193	T14191	pobj	cerebellum,in
R6602	T14194	T14193	prep	of,cerebellum
R6603	T14195	T14196	amod	adult,mice
R6604	T14196	T14194	pobj	mice,of
R6605	T14197	T14190	punct	.,immunostaining
R6606	T14199	T14200	punct	(,A
R6607	T14200	T14201	meta	A,presents
R6608	T14202	T14200	punct	),A
R6609	T14203	T14204	amod	Low,magnification
R6610	T14204	T14201	nsubj	magnification,presents
R6611	T14205	T14204	prep	of,magnification
R6612	T14206	T14207	det	the,cerebellum
R6613	T14207	T14205	pobj	cerebellum,of
R6614	T14208	T14209	det	an,expression
R6615	T14209	T14201	dobj	expression,presents
R6616	T14210	T14211	compound	Annexin,A7
R6617	T14211	T14209	compound	A7,expression
R6618	T14212	T14213	advmod	mainly,in
R6619	T14213	T14201	prep	in,presents
R6620	T14214	T14213	pobj	cells,in
R6621	T14215	T14214	prep	of,cells
R6622	T14216	T14217	det	the,granulosum
R6623	T14217	T14215	pobj	granulosum,of
R6624	T14218	T14217	compound	stratum,granulosum
R6625	T14219	T14217	cc	and,granulosum
R6626	T14220	T14221	compound	laminae,medullares
R6627	T14221	T14217	conj	medullares,granulosum
R6628	T14222	T14201	punct	.,presents
R6629	T14224	T14225	punct	(,B
R6630	T14225	T14226	meta	B,section
R6631	T14227	T14225	punct	),B
R6632	T14228	T14226	amod	Corresponding,section
R6633	T14229	T14226	acl	stained,section
R6634	T14230	T14229	prep	for,stained
R6635	T14231	T14230	pobj	GFAP,for
R6636	T14232	T14226	punct	.,section
R6637	T14234	T14235	punct	(,C
R6638	T14235	T14236	meta	C,magnification
R6639	T14237	T14235	punct	),C
R6640	T14238	T14236	amod	Higher,magnification
R6641	T14239	T14236	prep	of,magnification
R6642	T14240	T14239	pobj	folia,of
R6643	T14241	T14240	prep	of,folia
R6644	T14242	T14243	det	the,cerebellum
R6645	T14243	T14241	pobj	cerebellum,of
R6646	T14244	T14240	punct	", ",folia
R6647	T14245	T14246	advmod	where,used
R6648	T14246	T14240	relcl	used,folia
R6649	T14247	T14248	det	a,antibody
R6650	T14248	T14246	nsubjpass	antibody,used
R6651	T14249	T14248	amod	polyclonal,antibody
R6652	T14250	T14248	amod	anti-AnnexinA7,antibody
R6653	T14251	T14246	auxpass	was,used
R6654	T14252	T14236	punct	;,magnification
R6655	T14253	T14254	dep	square,given
R6656	T14254	T14236	dep	given,magnification
R6657	T14255	T14254	punct	", ",given
R6658	T14256	T14257	det	a,magnification
R6659	T14257	T14254	nsubjpass	magnification,given
R6660	T14258	T14257	amod	higher,magnification
R6661	T14259	T14257	prep	of,magnification
R6662	T14260	T14261	amod	acorresponding,area
R6663	T14261	T14259	pobj	area,of
R6664	T14262	T14261	acl	stained,area
R6665	T14263	T14262	prep	with,stained
R6666	T14264	T14265	nmod	mAb,203
R6667	T14265	T14263	pobj	203,with
R6668	T14266	T14267	punct	–,217
R6669	T14267	T14265	prep	217,203
R6670	T14268	T14254	auxpass	is,given
R6671	T14269	T14254	prep	in,given
R6672	T14270	T14269	punct	(,in
R6673	T14271	T14269	pobj	D,in
R6674	T14272	T14236	punct	),magnification
R6675	T14273	T14236	punct	.,magnification
R6676	T14275	T14276	prep	Between,observed
R6677	T14277	T14278	compound	stratum,granulosum
R6678	T14278	T14275	pobj	granulosum,Between
R6679	T14279	T14278	cc	and,granulosum
R6680	T14280	T14281	compound	stratum,moleculare
R6681	T14281	T14278	conj	moleculare,granulosum
R6682	T14282	T14283	det	the,layer
R6683	T14283	T14276	nsubjpass	layer,observed
R6684	T14284	T14283	prep	of,layer
R6685	T14285	T14286	compound	Purkinje,cells
R6686	T14286	T14284	pobj	cells,of
R6687	T14287	T14286	punct	-,cells
R6688	T14288	T14289	punct	(,piriformium
R6689	T14289	T14286	parataxis	piriformium,cells
R6690	T14290	T14289	compound	stratum,piriformium
R6691	T14291	T14289	compound	neuronorum,piriformium
R6692	T14292	T14293	punct	(,ganglionare
R6693	T14293	T14289	parataxis	ganglionare,piriformium
R6694	T14294	T14293	punct	),ganglionare
R6695	T14295	T14289	punct	),piriformium
R6696	T14296	T14276	aux	can,observed
R6697	T14297	T14276	auxpass	be,observed
R6698	T14298	T14276	punct	.,observed
R6699	T14300	T14301	punct	(,D
R6700	T14301	T14302	meta	D,Staining
R6701	T14303	T14301	punct	),D
R6702	T14304	T14302	prep	of,Staining
R6703	T14305	T14306	det	the,band
R6704	T14306	T14304	pobj	band,of
R6705	T14307	T14306	prep	of,band
R6706	T14308	T14309	compound	Purkinje,cells
R6707	T14309	T14307	pobj	cells,of
R6708	T14310	T14309	punct	-,cells
R6709	T14311	T14312	punct	(,arrows
R6710	T14312	T14302	parataxis	arrows,Staining
R6711	T14313	T14312	punct	),arrows
R6712	T14314	T14302	punct	.,Staining
R6713	T14316	T14317	det	The,stain
R6714	T14317	T14322	nsubj	stain,is
R6715	T14318	T14317	amod	positive,stain
R6716	T14319	T14320	compound	Annexin,A7
R6717	T14320	T14317	compound	A7,stain
R6718	T14321	T14317	punct	-,stain
R6719	T14323	T14317	prep	in,stain
R6720	T14324	T14325	det	the,granulosum
R6721	T14325	T14323	pobj	granulosum,in
R6722	T14326	T14325	compound	stratum,granulosum
R6723	T14327	T14322	prep	due,is
R6724	T14328	T14327	pcomp	to,due
R6725	T14329	T14327	pobj	staining,due
R6726	T14330	T14329	prep	of,staining
R6727	T14331	T14332	det	the,nuclei
R6728	T14332	T14330	pobj	nuclei,of
R6729	T14333	T14332	prep	of,nuclei
R6730	T14334	T14333	pobj	neurons,of
R6731	T14335	T14322	punct	.,is
R6732	T14337	T14338	punct	(,E
R6733	T14338	T14339	meta	E,is
R6734	T14340	T14338	punct	),E
R6735	T14341	T14339	prep	In,is
R6736	T14342	T14341	pobj	addition,In
R6737	T14343	T14342	prep	to,addition
R6738	T14344	T14345	det	an,staining
R6739	T14345	T14343	pobj	staining,to
R6740	T14346	T14345	amod	intense,staining
R6741	T14347	T14345	prep	of,staining
R6742	T14348	T14349	det	the,nucleus
R6743	T14349	T14347	pobj	nucleus,of
R6744	T14350	T14339	punct	", ",is
R6745	T14351	T14352	det	the,body
R6746	T14352	T14339	nsubj	body,is
R6747	T14353	T14352	compound	cell,body
R6748	T14354	T14355	npadvmod	AnnexinA7,positive
R6749	T14355	T14339	acomp	positive,is
R6750	T14356	T14355	punct	-,positive
R6751	T14357	T14339	prep	including,is
R6752	T14358	T14359	det	both,dendrites
R6753	T14359	T14357	pobj	dendrites,including
R6754	T14360	T14359	prep	of,dendrites
R6755	T14361	T14362	det	the,cell
R6756	T14362	T14360	pobj	cell,of
R6757	T14363	T14362	compound	Purkinje,cell
R6758	T14364	T14362	punct	-,cell
R6759	T14365	T14362	acl	shown,cell
R6760	T14366	T14339	punct	.,is
R6761	T14368	T14369	punct	(,F
R6762	T14369	T14370	meta	F,section
R6763	T14371	T14369	punct	),F
R6764	T14372	T14370	amod	Corresponding,section
R6765	T14373	T14370	prep	from,section
R6766	T14374	T14375	det	an,mouse
R6767	T14375	T14373	pobj	mouse,from
R6768	T14376	T14375	nmod	AnxA7,mouse
R6769	T14377	T14376	punct	-,AnxA7
R6770	T14378	T14376	punct	/,AnxA7
R6771	T14379	T14376	punct	-,AnxA7
R6772	T14380	T14370	punct	.,section
R6773	T14382	T14383	punct	(,G
R6774	T14383	T14384	meta	G,staining
R6775	T14385	T14383	punct	),G
R6776	T14386	T14384	compound	AnnexinA7,staining
R6777	T14387	T14384	prep	of,staining
R6778	T14388	T14387	pobj	axons,of
R6779	T14389	T14390	punct	(,arrowheads
R6780	T14390	T14388	parataxis	arrowheads,axons
R6781	T14391	T14390	punct	),arrowheads
R6782	T14392	T14388	acl	running,axons
R6783	T14393	T14392	prep	from,running
R6784	T14394	T14395	det	the,medullares
R6785	T14395	T14393	pobj	medullares,from
R6786	T14396	T14395	compound	laminae,medullares
R6787	T14397	T14392	prep	to,running
R6788	T14398	T14399	det	the,layer
R6789	T14399	T14397	pobj	layer,to
R6790	T14400	T14401	compound	Purkinje,cell
R6791	T14401	T14399	compound	cell,layer
R6792	T14402	T14401	punct	-,cell
R6793	T14403	T14399	acl	located,layer
R6794	T14404	T14403	prep	in,located
R6795	T14405	T14406	det	the,end
R6796	T14406	T14404	pobj	end,in
R6797	T14407	T14406	amod	round,end
R6798	T14408	T14406	prep	of,end
R6799	T14409	T14410	det	a,convolution
R6800	T14410	T14408	pobj	convolution,of
R6801	T14411	T14384	punct	.,staining
R6802	T14413	T14414	nmod	Sections,G
R6803	T14414	T14423	nsubjpass	G,stained
R6804	T14415	T14414	nmod	A,G
R6805	T14416	T14414	punct	", ",G
R6806	T14417	T14414	nmod	D,G
R6807	T14418	T14414	punct	", ",G
R6808	T14419	T14414	nmod	E,G
R6809	T14420	T14414	punct	", ",G
R6810	T14421	T14414	nmod	F,G
R6811	T14422	T14414	punct	", ",G
R6812	T14424	T14423	auxpass	were,stained
R6813	T14425	T14423	prep	with,stained
R6814	T14426	T14427	nmod	mAb,203
R6815	T14427	T14425	pobj	203,with
R6816	T14428	T14429	punct	–,217
R6817	T14429	T14427	prep	217,203
R6818	T14430	T14423	punct	.,stained
R6819	T14500	T14501	compound	Annexin,A7
R6820	T14501	T14502	compound	A7,immunostaining
R6821	T14503	T14502	prep	in,immunostaining
R6822	T14504	T14505	amod	human,isocortex
R6823	T14505	T14503	pobj	isocortex,in
R6824	T14506	T14502	punct	.,immunostaining
R6825	T14508	T14509	punct	(,A
R6826	T14509	T14510	meta	A,labeled
R6827	T14511	T14509	punct	),A
R6828	T14512	T14513	amod	Pyramidal,neurons
R6829	T14513	T14510	nsubjpass	neurons,labeled
R6830	T14514	T14515	punct	(,arrow
R6831	T14515	T14513	parataxis	arrow,neurons
R6832	T14516	T14515	amod	bold,arrow
R6833	T14517	T14515	punct	),arrow
R6834	T14518	T14513	cc	and,neurons
R6835	T14519	T14520	amod	apical,dendrites
R6836	T14520	T14513	conj	dendrites,neurons
R6837	T14521	T14522	punct	(,arrows
R6838	T14522	T14520	parataxis	arrows,dendrites
R6839	T14523	T14522	amod	small,arrows
R6840	T14524	T14522	punct	),arrows
R6841	T14525	T14510	auxpass	were,labeled
R6842	T14526	T14510	advmod	clearly,labeled
R6843	T14527	T14510	prep	with,labeled
R6844	T14528	T14529	det	the,antibody
R6845	T14529	T14527	pobj	antibody,with
R6846	T14530	T14529	prep	against,antibody
R6847	T14531	T14532	compound	Annexin,A7
R6848	T14532	T14530	pobj	A7,against
R6849	T14533	T14510	prep	in,labeled
R6850	T14534	T14535	det	the,cortex
R6851	T14535	T14533	pobj	cortex,in
R6852	T14536	T14535	amod	human,cortex
R6853	T14537	T14535	amod	parietal,cortex
R6854	T14538	T14510	punct	.,labeled
R6855	T14540	T14541	punct	(,B
R6856	T14541	T14542	meta	B,seen
R6857	T14543	T14541	punct	),B
R6858	T14544	T14545	det	A,staining
R6859	T14545	T14542	nsubjpass	staining,seen
R6860	T14546	T14545	amod	dendritic,staining
R6861	T14547	T14542	auxpass	was,seen
R6862	T14548	T14542	advmod	also,seen
R6863	T14549	T14542	prep	in,seen
R6864	T14550	T14551	det	the,layer
R6865	T14551	T14549	pobj	layer,in
R6866	T14552	T14551	amod	molecular,layer
R6867	T14553	T14554	punct	(,arrows
R6868	T14554	T14542	parataxis	arrows,seen
R6869	T14555	T14554	amod	small,arrows
R6870	T14556	T14554	punct	),arrows
R6871	T14557	T14542	punct	.,seen
R6872	T14559	T14560	prep	In,labeled
R6873	T14561	T14559	pobj	addition,In
R6874	T14562	T14563	det	the,astrocytes
R6875	T14563	T14560	nsubjpass	astrocytes,labeled
R6876	T14564	T14563	amod	subpial,astrocytes
R6877	T14565	T14560	auxpass	were,labeled
R6878	T14566	T14560	advmod	weakly,labeled
R6879	T14567	T14568	punct	(,arrows
R6880	T14568	T14560	parataxis	arrows,labeled
R6881	T14569	T14570	amod	double,headed
R6882	T14570	T14568	amod	headed,arrows
R6883	T14571	T14568	punct	),arrows
R6884	T14572	T14560	punct	.,labeled
R6885	T14574	T14575	det	The,staining
R6886	T14575	T14576	nsubj	staining,was
R6887	T14576	T14577	ccomp	was,showed
R6888	T14578	T14576	acomp	cytoplasmic,was
R6889	T14579	T14577	punct	", ",showed
R6890	T14580	T14581	advmod	only,astrocytes
R6891	T14581	T14577	nsubj	astrocytes,showed
R6892	T14582	T14581	amod	few,astrocytes
R6893	T14583	T14584	det	a,staining
R6894	T14584	T14577	dobj	staining,showed
R6895	T14585	T14584	amod	nuclear,staining
R6896	T14586	T14587	advmod	as,well
R6897	T14587	T14577	advmod	well,showed
R6898	T14588	T14589	punct	(,inset
R6899	T14589	T14577	parataxis	inset,showed
R6900	T14590	T14589	punct	),inset
R6901	T14591	T14577	punct	.,showed
R6902	T14593	T14594	punct	(,C
R6903	T14594	T14595	meta	C,seen
R6904	T14596	T14594	punct	),C
R6905	T14597	T14595	dep	Enlargement,seen
R6906	T14598	T14597	prep	of,Enlargement
R6907	T14599	T14598	punct	(,of
R6908	T14600	T14598	pobj	A,of
R6909	T14601	T14595	punct	),seen
R6910	T14602	T14595	punct	: ,seen
R6911	T14603	T14604	compound	Annexin,A7
R6912	T14604	T14595	nsubjpass	A7,seen
R6913	T14605	T14595	auxpass	was,seen
R6914	T14606	T14595	prep	at,seen
R6915	T14607	T14608	det	the,membrane
R6916	T14608	T14606	pobj	membrane,at
R6917	T14609	T14608	compound	cell,membrane
R6918	T14610	T14608	prep	of,membrane
R6919	T14611	T14612	det	the,perikaryon
R6920	T14612	T14610	pobj	perikaryon,of
R6921	T14613	T14614	punct	(,arrow
R6922	T14614	T14612	parataxis	arrow,perikaryon
R6923	T14615	T14614	amod	bold,arrow
R6924	T14616	T14614	punct	),arrow
R6925	T14617	T14612	cc	and,perikaryon
R6926	T14618	T14619	det	the,dendrite
R6927	T14619	T14612	conj	dendrite,perikaryon
R6928	T14620	T14619	amod	apical,dendrite
R6929	T14621	T14622	punct	(,arrows
R6930	T14622	T14619	parataxis	arrows,dendrite
R6931	T14623	T14622	amod	small,arrows
R6932	T14624	T14622	punct	),arrows
R6933	T14625	T14595	punct	.,seen
R6934	T14627	T14628	punct	(,D
R6935	T14628	T14629	meta	D,show
R6936	T14630	T14628	punct	),D
R6937	T14631	T14632	det	A,control
R6938	T14632	T14629	nsubj	control,show
R6939	T14633	T14632	amod	blank,control
R6940	T14634	T14632	acl	lacking,control
R6941	T14635	T14636	det	the,antibody
R6942	T14636	T14634	dobj	antibody,lacking
R6943	T14637	T14636	amod	primary,antibody
R6944	T14638	T14629	aux	did,show
R6945	T14639	T14629	neg	not,show
R6946	T14640	T14641	det	a,labeling
R6947	T14641	T14629	dobj	labeling,show
R6948	T14642	T14641	amod	specific,labeling
R6949	T14643	T14629	punct	", ",show
R6950	T14644	T14629	cc	but,show
R6951	T14645	T14646	amod	autofluorescent,lipofuscin
R6952	T14646	T14647	nsubj	lipofuscin,was
R6953	T14647	T14629	conj	was,show
R6954	T14648	T14647	acomp	detectable,was
R6955	T14649	T14647	prep	in,was
R6956	T14650	T14651	det	the,neurons
R6957	T14651	T14649	pobj	neurons,in
R6958	T14652	T14653	punct	(,arrowheads
R6959	T14653	T14647	parataxis	arrowheads,was
R6960	T14654	T14653	punct	),arrowheads
R6961	T14655	T14647	punct	.,was
R6962	T14658	T14657	punct	", ",Bar
R6963	T14659	T14660	punct	(,A
R6964	T14660	T14661	meta	A,μm
R6965	T14661	T14657	appos	μm,Bar
R6966	T14662	T14660	punct	),A
R6967	T14663	T14661	nummod	70,μm
R6968	T14664	T14661	punct	", ",μm
R6969	T14665	T14666	punct	(,B
R6970	T14666	T14667	meta	B,μm
R6971	T14667	T14661	appos	μm,μm
R6972	T14668	T14666	punct	),B
R6973	T14669	T14667	nummod	50,μm
R6974	T14670	T14661	punct	", ",μm
R6975	T14671	T14672	punct	(,B
R6976	T14672	T14673	meta	B,μm
R6977	T14673	T14661	appos	μm,μm
R6978	T14674	T14672	punct	-,B
R6979	T14675	T14672	dep	inset,B
R6980	T14676	T14672	punct	", ",B
R6981	T14677	T14672	dep	C,B
R6982	T14678	T14672	punct	),B
R6983	T14679	T14673	nummod	17,μm
R6984	T14680	T14661	punct	", ",μm
R6985	T14681	T14682	punct	(,D
R6986	T14682	T14683	meta	D,μm
R6987	T14683	T14661	appos	μm,μm
R6988	T14684	T14682	punct	),D
R6989	T14685	T14683	nummod	20,μm
R6990	T14686	T14657	punct	.,Bar
R6997	T14995	T14996	amod	Nuclear,localization
R6998	T14997	T14996	prep	of,localization
R6999	T14998	T14999	compound	Annexin,A7
R7000	T14999	T14997	pobj	A7,of
R7001	T15000	T14996	punct	.,localization
R7002	T15002	T15003	punct	(,A
R7003	T15003	T15004	meta	A,Extraction
R7004	T15005	T15003	punct	),A
R7005	T15006	T15004	prep	of,Extraction
R7006	T15007	T15008	compound	Annexin,A7
R7007	T15008	T15006	pobj	A7,of
R7008	T15009	T15004	prep	from,Extraction
R7009	T15010	T15009	pobj	nuclei,from
R7010	T15011	T15010	prep	of,nuclei
R7011	T15012	T15011	pobj	neuronal,of
R7012	T15013	T15014	punct	(,PC
R7013	T15014	T15012	parataxis	PC,neuronal
R7014	T15015	T15014	punct	-,PC
R7015	T15016	T15014	nummod	12,PC
R7016	T15017	T15014	punct	", ",PC
R7017	T15018	T15014	appos	N2A,PC
R7018	T15019	T15014	punct	),PC
R7019	T15020	T15012	cc	and,neuronal
R7020	T15021	T15022	det	an,astroglial
R7021	T15022	T15023	nmod	astroglial,line
R7022	T15023	T15012	conj	line,neuronal
R7023	T15024	T15025	punct	(,C6
R7024	T15025	T15022	parataxis	C6,astroglial
R7025	T15026	T15025	punct	),C6
R7026	T15027	T15023	compound	cell,line
R7027	T15028	T15004	acl	using,Extraction
R7028	T15029	T15030	det	a,buffer
R7029	T15030	T15028	dobj	buffer,using
R7030	T15031	T15030	amod	hypotonic,buffer
R7031	T15032	T15004	punct	.,Extraction
R7032	T15034	T15035	nsubjpass	Samples,subjected
R7033	T15036	T15034	prep	of,Samples
R7034	T15037	T15038	amod	total,cells
R7035	T15038	T15036	pobj	cells,of
R7036	T15039	T15040	punct	(,c
R7037	T15040	T15034	parataxis	c,Samples
R7038	T15041	T15040	punct	),c
R7039	T15042	T15034	punct	", ",Samples
R7040	T15043	T15034	cc	and,Samples
R7041	T15044	T15045	amod	corresponding,amounts
R7042	T15045	T15034	conj	amounts,Samples
R7043	T15046	T15045	prep	of,amounts
R7044	T15047	T15048	amod	nuclear,membranes
R7045	T15048	T15046	pobj	membranes,of
R7046	T15049	T15050	punct	(,m
R7047	T15050	T15048	parataxis	m,membranes
R7048	T15051	T15050	punct	),m
R7049	T15052	T15048	cc	and,membranes
R7050	T15053	T15048	conj	nucleoplasm,membranes
R7051	T15054	T15055	punct	(,p
R7052	T15055	T15053	parataxis	p,nucleoplasm
R7053	T15056	T15055	punct	),p
R7054	T15057	T15035	auxpass	were,subjected
R7055	T15058	T15035	prep	to,subjected
R7056	T15059	T15060	compound	SDS,PAGE
R7057	T15060	T15058	pobj	PAGE,to
R7058	T15061	T15060	punct	-,PAGE
R7059	T15062	T15060	cc	and,PAGE
R7060	T15063	T15064	compound	Western,blotting
R7061	T15064	T15060	conj	blotting,PAGE
R7062	T15065	T15035	punct	.,subjected
R7063	T15067	T15068	nmod	Annexin,A7
R7064	T15068	T15069	nmod	A7,isoforms
R7065	T15069	T15073	nsubj	isoforms,are
R7066	T15070	T15068	punct	(,A7
R7067	T15071	T15068	appos	AnxA7,A7
R7068	T15072	T15069	punct	),isoforms
R7069	T15074	T15073	acomp	extractable,are
R7070	T15075	T15074	prep	from,extractable
R7071	T15076	T15077	det	the,nucleus
R7072	T15077	T15075	pobj	nucleus,from
R7073	T15078	T15077	prep	of,nucleus
R7074	T15079	T15080	det	the,cells
R7075	T15080	T15078	pobj	cells,of
R7076	T15081	T15080	amod	indicated,cells
R7077	T15082	T15083	punct	(,arrowhead
R7078	T15083	T15080	parataxis	arrowhead,cells
R7079	T15084	T15085	nummod	47,kDa
R7080	T15085	T15086	nmod	kDa,isoform
R7081	T15086	T15083	dep	isoform,arrowhead
R7082	T15087	T15085	cc	and,kDa
R7083	T15088	T15089	nummod	51,kDa
R7084	T15089	T15085	conj	kDa,kDa
R7085	T15090	T15083	punct	", ",arrowhead
R7086	T15091	T15083	punct	),arrowhead
R7087	T15092	T15073	punct	.,are
R7088	T15094	T15095	prep	For,shown
R7089	T15096	T15094	pobj	control,For
R7090	T15097	T15095	punct	", ",shown
R7091	T15098	T15095	nsubjpass	immunoblotting,shown
R7092	T15099	T15098	prep	of,immunoblotting
R7093	T15100	T15099	pobj	LAP2α,of
R7094	T15101	T15102	punct	(,isoform
R7095	T15102	T15100	parataxis	isoform,LAP2α
R7096	T15103	T15104	nummod	75,kDa
R7097	T15104	T15102	dep	kDa,isoform
R7098	T15105	T15102	punct	;,isoform
R7099	T15106	T15107	amod	non-membrane,bound
R7100	T15107	T15102	amod	bound,isoform
R7101	T15108	T15107	punct	-,bound
R7102	T15109	T15102	punct	),isoform
R7103	T15110	T15100	punct	", ",LAP2α
R7104	T15111	T15100	conj	Emerin,LAP2α
R7105	T15112	T15111	punct	(,Emerin
R7106	T15113	T15111	appos	EM,Emerin
R7107	T15114	T15115	punct	", ",kDa
R7108	T15115	T15111	parataxis	kDa,Emerin
R7109	T15116	T15115	nummod	34,kDa
R7110	T15117	T15115	punct	),kDa
R7111	T15118	T15111	punct	", ",Emerin
R7112	T15119	T15111	cc	and,Emerin
R7113	T15120	T15111	conj	tubulin,Emerin
R7114	T15121	T15120	punct	(,tubulin
R7115	T15122	T15120	appos	TB,tubulin
R7116	T15123	T15124	punct	", ",kDa
R7117	T15124	T15120	parataxis	kDa,tubulin
R7118	T15125	T15124	nummod	55,kDa
R7119	T15126	T15124	punct	),kDa
R7120	T15127	T15128	dep	which,are
R7121	T15128	T15100	relcl	are,LAP2α
R7122	T15129	T15128	acomp	specific,are
R7123	T15130	T15129	prep	for,specific
R7124	T15131	T15130	pobj	nucleoplasm,for
R7125	T15132	T15133	punct	(,LAP2α
R7126	T15133	T15131	parataxis	LAP2α,nucleoplasm
R7127	T15134	T15133	punct	),LAP2α
R7128	T15135	T15100	punct	", ",LAP2α
R7129	T15136	T15137	amod	nuclear,membranes
R7130	T15137	T15100	appos	membranes,LAP2α
R7131	T15138	T15139	punct	(,Emerin
R7132	T15139	T15137	parataxis	Emerin,membranes
R7133	T15140	T15139	punct	),Emerin
R7134	T15141	T15137	punct	", ",membranes
R7135	T15142	T15137	cc	and,membranes
R7136	T15143	T15144	det	the,cytoplasm
R7137	T15144	T15137	conj	cytoplasm,membranes
R7138	T15145	T15146	punct	(,tubulin
R7139	T15146	T15144	parataxis	tubulin,cytoplasm
R7140	T15147	T15146	punct	),tubulin
R7141	T15148	T15095	auxpass	are,shown
R7142	T15149	T15095	punct	.,shown
R7143	T15152	T15153	mark	that,is
R7144	T15153	T15151	ccomp	is,Note
R7145	T15154	T15155	det	the,nucleoplasm
R7146	T15155	T15153	nsubj	nucleoplasm,is
R7147	T15156	T15157	advmod	only,partially
R7148	T15157	T15158	advmod	partially,extractable
R7149	T15158	T15153	acomp	extractable,is
R7150	T15159	T15151	punct	.,Note
R7151	T15161	T15162	punct	(,B
R7152	T15162	T15163	meta	B,images
R7153	T15164	T15162	punct	),B
R7154	T15165	T15163	compound	Immunofluorescence,images
R7155	T15166	T15163	prep	of,images
R7156	T15167	T15168	det	the,cells
R7157	T15168	T15166	pobj	cells,of
R7158	T15169	T15168	nmod	PC,cells
R7159	T15170	T15169	punct	-,PC
R7160	T15171	T15169	nummod	12,PC
R7161	T15172	T15168	acl	used,cells
R7162	T15173	T15163	punct	.,images
R7163	T15175	T15176	nsubjpass	Cells,fixed
R7164	T15177	T15176	auxpass	were,fixed
R7165	T15178	T15176	prep	with,fixed
R7166	T15179	T15178	pobj	paraformaldehyde,with
R7167	T15180	T15176	punct	", ",fixed
R7168	T15181	T15176	conj	permeabilized,fixed
R7169	T15182	T15181	prep	with,permeabilized
R7170	T15183	T15184	compound	Triton,X
R7171	T15184	T15182	pobj	X,with
R7172	T15185	T15184	punct	-,X
R7173	T15186	T15184	nummod	100,X
R7174	T15187	T15181	punct	", ",permeabilized
R7175	T15188	T15181	conj	pretreated,permeabilized
R7176	T15189	T15188	prep	with,pretreated
R7177	T15190	T15189	pobj	trypsin,with
R7178	T15191	T15192	mark	as,indicated
R7179	T15192	T15188	advcl	indicated,pretreated
R7180	T15193	T15188	punct	", ",pretreated
R7181	T15194	T15188	cc	and,pretreated
R7182	T15195	T15188	conj	stained,pretreated
R7183	T15196	T15195	prep	with,stained
R7184	T15197	T15198	compound	Annexin,A7
R7185	T15198	T15199	npadvmod	A7,specific
R7186	T15199	T15200	amod	specific,203
R7187	T15200	T15196	pobj	203,with
R7188	T15201	T15200	nmod	mAb,203
R7189	T15202	T15203	punct	–,217
R7190	T15203	T15200	prep	217,203
R7191	T15204	T15205	punct	", ",μm
R7192	T15205	T15195	parataxis	μm,stained
R7193	T15206	T15205	dep	DAPI,μm
R7194	T15207	T15206	prep	in,DAPI
R7195	T15208	T15207	amod	blue,in
R7196	T15209	T15205	punct	", ",μm
R7197	T15210	T15205	dep	bar,μm
R7198	T15211	T15205	nummod	10,μm
R7199	T15212	T15176	punct	.,fixed
R771	T2289	T2290	compound	Annexin,A7
R772	T2290	T2291	nsubjpass	A7,expressed
R773	T2292	T2291	auxpass	is,expressed
R774	T2293	T2291	prep	in,expressed
R775	T2294	T2295	det	the,embryo
R776	T2295	T2293	pobj	embryo,in
R777	T2296	T2295	amod	early,embryo
R778	T2297	T2295	compound	mouse,embryo
R779	T2299	T2300	advmod	First,examined
R780	T2301	T2300	nsubj	we,examined
R781	T2302	T2303	det	the,expression
R782	T2303	T2300	dobj	expression,examined
R783	T2304	T2303	prep	of,expression
R784	T2305	T2306	compound	Annexin,A7
R785	T2306	T2304	pobj	A7,of
R786	T2307	T2303	prep	in,expression
R787	T2308	T2309	compound	ES,cells
R788	T2309	T2307	pobj	cells,in
R789	T2310	T2311	punct	(,Bruce4
R790	T2311	T2309	parataxis	Bruce4,cells
R791	T2312	T2311	punct	", ",Bruce4
R792	T2313	T2309	acl	established,cells
R793	T2314	T2313	prep	from,established
R794	T2315	T2316	compound	C57BL,6J
R795	T2316	T2318	compound	6J,mice
R796	T2317	T2316	punct	/,6J
R797	T2318	T2314	pobj	mice,from
R798	T2319	T2309	punct	),cells
R799	T2320	T2309	cc	and,cells
R800	T2321	T2322	det	the,stages
R801	T2322	T2309	conj	stages,cells
R802	T2323	T2322	amod	early,stages
R803	T2324	T2322	prep	of,stages
R804	T2325	T2326	nmod	mouse,development
R805	T2326	T2324	pobj	development,of
R806	T2327	T2326	amod	embryonic,development
R807	T2328	T2300	prep	at,examined
R808	T2329	T2330	det	the,level
R809	T2330	T2328	pobj	level,at
R810	T2331	T2330	compound	mRNA,level
R811	T2332	T2300	prep	by,examined
R812	T2333	T2334	compound	northern,blot
R813	T2334	T2335	compound	blot,analysis
R814	T2335	T2332	pobj	analysis,by
R815	T2336	T2300	cc	and,examined
R816	T2337	T2300	conj	at,examined
R817	T2338	T2339	det	the,level
R818	T2339	T2337	pobj	level,at
R819	T2340	T2339	compound	protein,level
R820	T2341	T2337	prep	by,at
R821	T2342	T2343	compound	Western,blotting
R822	T2343	T2341	pobj	blotting,by
R823	T2344	T2343	cc	and,blotting
R824	T2345	T2343	conj	immunohistochemistry,blotting
R825	T2346	T2337	punct	", ",at
R826	T2347	T2337	conj	respectively,at
R827	T2348	T2300	punct	.,examined
R828	T2350	T2351	compound	Northern,blot
R829	T2351	T2352	compound	blot,analysis
R830	T2352	T2353	nsubj	analysis,shows
R831	T2354	T2353	prep	in,shows
R832	T2355	T2356	compound	ES,cells
R833	T2356	T2354	pobj	cells,in
R834	T2357	T2356	punct	-,cells
R835	T2358	T2354	cc	and,in
R836	T2359	T2354	conj	at,in
R837	T2360	T2361	amod	embryonic,E7
R838	T2361	T2359	pobj	E7,at
R839	T2362	T2361	compound	days,E7
R840	T2363	T2361	punct	", ",E7
R841	T2364	T2361	conj	E11,E7
R842	T2365	T2364	punct	", ",E11
R843	T2366	T2364	conj	E15,E11
R844	T2367	T2366	punct	", ",E15
R845	T2368	T2366	cc	and,E15
R846	T2369	T2366	conj	E17,E15
R847	T2370	T2371	nummod	two,species
R848	T2371	T2353	dobj	species,shows
R849	T2372	T2371	compound	mRNA,species
R850	T2373	T2371	prep	of,species
R851	T2374	T2375	nummod	1.8,kb
R852	T2375	T2373	pobj	kb,of
R853	T2376	T2375	cc	and,kb
R854	T2377	T2378	nummod	2.4,kb
R855	T2378	T2375	conj	kb,kb
R856	T2379	T2371	punct	", ",species
R857	T2380	T2381	dep	which,result
R858	T2381	T2371	relcl	result,species
R859	T2382	T2381	prep	from,result
R860	T2383	T2384	amod	alternative,splicing
R861	T2384	T2382	pobj	splicing,from
R862	T2385	T2381	prep	in,result
R863	T2386	T2387	det	the,end
R864	T2387	T2385	pobj	end,in
R865	T2388	T2387	amod	untranslated,end
R866	T2389	T2387	nummod	3,end
R867	T2390	T2389	punct	',3
R868	T2391	T2392	punct	(,Fig.
R869	T2392	T2353	parataxis	Fig.,shows
R870	T2393	T2392	nummod	1A,Fig.
R871	T2394	T2392	punct	),Fig.
R872	T2395	T2396	punct	[,11
R873	T2396	T2353	parataxis	11,shows
R874	T2397	T2396	punct	],11
R875	T2398	T2353	punct	.,shows
R876	T2400	T2401	nsubj	We,found
R877	T2402	T2403	amod	similar,levels
R878	T2403	T2401	dobj	levels,found
R879	T2404	T2405	compound	Annexin,A7
R880	T2405	T2403	compound	A7,levels
R881	T2406	T2403	compound	mRNA,levels
R882	T2407	T2401	prep	in,found
R883	T2408	T2409	det	the,stages
R884	T2409	T2407	pobj	stages,in
R885	T2410	T2409	nummod	four,stages
R886	T2411	T2409	amod	embryonic,stages
R887	T2412	T2401	punct	.,found
R888	T2414	T2415	nsubj	Reprobing,verified
R889	T2415	T2422	ccomp	verified,is
R890	T2416	T2414	prep	with,Reprobing
R891	T2417	T2418	det	a,probe
R892	T2418	T2416	pobj	probe,with
R893	T2419	T2420	compound	β,actin
R894	T2420	T2418	compound	actin,probe
R895	T2421	T2420	punct	-,actin
R896	T2423	T2424	amod	equal,loading
R897	T2424	T2415	dobj	loading,verified
R898	T2425	T2422	punct	;,is
R899	T2426	T2427	det	the,appearance
R900	T2427	T2422	nsubj	appearance,is
R901	T2428	T2427	prep	of,appearance
R902	T2429	T2430	det	a,species
R903	T2430	T2428	pobj	species,of
R904	T2431	T2432	amod	faster,migrating
R905	T2432	T2430	amod	migrating,species
R906	T2433	T2430	compound	mRNA,species
R907	T2434	T2430	prep	in,species
R908	T2435	T2434	pobj	addition,in
R909	T2436	T2435	prep	to,addition
R910	T2437	T2438	det	the,species
R911	T2438	T2436	pobj	species,to
R912	T2439	T2440	nummod	2.0,kb
R913	T2440	T2438	compound	kb,species
R914	T2441	T2422	acomp	characteristic,is
R915	T2442	T2422	prep	for,is
R916	T2443	T2444	compound	β,actin
R917	T2444	T2442	pobj	actin,for
R918	T2445	T2444	punct	-,actin
R919	T2446	T2447	punct	[,12
R920	T2447	T2422	parataxis	12,is
R921	T2448	T2447	punct	],12
R922	T2449	T2422	punct	.,is
R923	T2451	T2452	prep	On,express
R924	T2453	T2454	det	the,level
R925	T2454	T2451	pobj	level,On
R926	T2455	T2454	compound	protein,level
R927	T2456	T2452	punct	", ",express
R928	T2457	T2458	compound	ES,cells
R929	T2458	T2452	nsubj	cells,express
R930	T2459	T2460	advmod	only,isoform
R931	T2460	T2452	dobj	isoform,express
R932	T2461	T2460	det	the,isoform
R933	T2462	T2460	amod	smaller,isoform
R934	T2463	T2464	compound	Annexin,A7
R935	T2464	T2460	compound	A7,isoform
R936	T2465	T2460	prep	of,isoform
R937	T2466	T2467	nummod	47,kDa
R938	T2467	T2465	pobj	kDa,of
R939	T2468	T2469	punct	(,1B
R940	T2469	T2452	parataxis	1B,express
R941	T2470	T2469	compound	Fig.,1B
R942	T2471	T2469	punct	", ",1B
R943	T2472	T2473	nmod	neuro,cells
R944	T2473	T2476	nsubjpass	cells,included
R945	T2474	T2472	punct	-,neuro
R946	T2475	T2472	nummod	2a,neuro
R947	T2476	T2469	ccomp	included,1B
R948	T2477	T2473	cc	and,cells
R949	T2478	T2479	compound	heart,tissue
R950	T2479	T2473	conj	tissue,cells
R951	T2480	T2476	auxpass	are,included
R952	T2481	T2476	prep	for,included
R953	T2482	T2481	pobj	control,for
R954	T2483	T2469	punct	),1B
R955	T2484	T2452	punct	.,express
R956	T2486	T2487	nsubj	Immunohistochemistry,confirmed
R957	T2488	T2489	det	the,presence
R958	T2489	T2487	dobj	presence,confirmed
R959	T2490	T2489	prep	of,presence
R960	T2491	T2492	det	the,protein
R961	T2492	T2490	pobj	protein,of
R962	T2493	T2489	prep	during,presence
R963	T2494	T2495	amod	early,development
R964	T2495	T2493	pobj	development,during
R965	T2496	T2487	punct	.,confirmed
R966	T2498	T2499	compound	Annexin,A7
R967	T2499	T2500	compound	A7,immunoreactivity
R968	T2500	T2501	nsubjpass	immunoreactivity,observed
R969	T2502	T2500	acl	using,immunoreactivity
R970	T2503	T2504	nmod	mAb,203
R971	T2504	T2502	dobj	203,using
R972	T2505	T2506	punct	–,217
R973	T2506	T2504	prep	217,203
R974	T2507	T2504	punct	", ",203
R975	T2508	T2509	dep	which,recognizes
R976	T2509	T2504	relcl	recognizes,203
R977	T2510	T2509	advmod	specifically,recognizes
R978	T2511	T2512	det	both,isoforms
R979	T2512	T2509	dobj	isoforms,recognizes
R980	T2513	T2512	prep	of,isoforms
R981	T2514	T2515	compound	Annexin,A7
R982	T2515	T2513	pobj	A7,of
R983	T2516	T2501	punct	", ",observed
R984	T2517	T2501	aux	can,observed
R985	T2518	T2501	auxpass	be,observed
R986	T2519	T2501	prep	in,observed
R987	T2520	T2521	det	the,types
R988	T2521	T2519	pobj	types,in
R989	T2522	T2521	nummod	two,types
R990	T2523	T2521	compound	cell,types
R991	T2524	T2521	prep	of,types
R992	T2525	T2526	det	the,stage
R993	T2526	T2524	pobj	stage,of
R994	T2527	T2526	compound	cylinder,stage
R995	T2528	T2501	prep	at,observed
R996	T2529	T2528	pobj	E5,at
R997	T2530	T2501	prep	with,observed
R998	T2531	T2532	det	a,expression
R999	T2532	T2530	pobj	expression,with

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PMC:1087847 / 4467-22347 JSON TXT 5 Projects