Id |
Subject |
Object |
Predicate |
Lexical cue |
T11182 |
0-6 |
NN |
denotes |
Embryo |
T11184 |
7-15 |
JJ |
denotes |
Multiple |
T11185 |
16-22 |
NN |
denotes |
Tissue |
T11186 |
23-31 |
NNP |
denotes |
Northern |
T11183 |
32-36 |
NN |
denotes |
Blot |
T11187 |
36-159 |
sentence |
denotes |
The presence of Annexin A7 mRNA in mouse ES cells and embryos E7, E11, E15 and E17 was analyzed by northern blot analysis. |
T11188 |
37-40 |
DT |
denotes |
The |
T11189 |
41-49 |
NN |
denotes |
presence |
T11191 |
50-52 |
IN |
denotes |
of |
T11192 |
53-60 |
NN |
denotes |
Annexin |
T11193 |
61-63 |
NN |
denotes |
A7 |
T11194 |
64-68 |
NN |
denotes |
mRNA |
T11195 |
69-71 |
IN |
denotes |
in |
T11196 |
72-77 |
NN |
denotes |
mouse |
T11198 |
78-80 |
NN |
denotes |
ES |
T11197 |
81-86 |
NNS |
denotes |
cells |
T11199 |
87-90 |
CC |
denotes |
and |
T11200 |
91-98 |
NNS |
denotes |
embryos |
T11201 |
99-101 |
NN |
denotes |
E7 |
T11202 |
101-103 |
, |
denotes |
, |
T11203 |
103-106 |
NN |
denotes |
E11 |
T11204 |
106-108 |
, |
denotes |
, |
T11205 |
108-111 |
NN |
denotes |
E15 |
T11206 |
112-115 |
CC |
denotes |
and |
T11207 |
116-119 |
NN |
denotes |
E17 |
T11208 |
120-123 |
VBD |
denotes |
was |
T11190 |
124-132 |
VBN |
denotes |
analyzed |
T11209 |
133-135 |
IN |
denotes |
by |
T11210 |
136-144 |
NNP |
denotes |
northern |
T11211 |
145-149 |
NN |
denotes |
blot |
T11212 |
150-158 |
NN |
denotes |
analysis |
T11213 |
158-159 |
. |
denotes |
. |
T11214 |
159-267 |
sentence |
denotes |
An Embryo Multiple Tissue Northern Blot membrane was obtained from Clontech Laboratories (Heidelberg, FRG). |
T11215 |
160-162 |
DT |
denotes |
An |
T11217 |
163-169 |
NN |
denotes |
Embryo |
T11218 |
170-178 |
JJ |
denotes |
Multiple |
T11219 |
179-185 |
NN |
denotes |
Tissue |
T11220 |
186-194 |
NNP |
denotes |
Northern |
T11221 |
195-199 |
NN |
denotes |
Blot |
T11216 |
200-208 |
NN |
denotes |
membrane |
T11223 |
209-212 |
VBD |
denotes |
was |
T11222 |
213-221 |
VBN |
denotes |
obtained |
T11224 |
222-226 |
IN |
denotes |
from |
T11225 |
227-235 |
NNP |
denotes |
Clontech |
T11226 |
236-248 |
NNP |
denotes |
Laboratories |
T11227 |
249-250 |
-LRB- |
denotes |
( |
T11228 |
250-260 |
NNP |
denotes |
Heidelberg |
T11229 |
260-262 |
, |
denotes |
, |
T11230 |
262-265 |
NNP |
denotes |
FRG |
T11231 |
265-266 |
-RRB- |
denotes |
) |
T11232 |
266-267 |
. |
denotes |
. |
T11233 |
267-373 |
sentence |
denotes |
It contained approximately 2 μg of poly(A)+ RNA per lane from four different stages of mouse development. |
T11234 |
268-270 |
PRP |
denotes |
It |
T11235 |
271-280 |
VBD |
denotes |
contained |
T11236 |
281-294 |
RB |
denotes |
approximately |
T11237 |
295-296 |
CD |
denotes |
2 |
T11238 |
297-299 |
NN |
denotes |
μg |
T11239 |
300-302 |
IN |
denotes |
of |
T11240 |
303-307 |
NN |
denotes |
poly |
T11242 |
307-308 |
-LRB- |
denotes |
( |
T11243 |
308-309 |
NN |
denotes |
A |
T11244 |
309-310 |
-RRB- |
denotes |
) |
T11245 |
310-311 |
SYM |
denotes |
+ |
T11241 |
312-315 |
NN |
denotes |
RNA |
T11246 |
316-319 |
IN |
denotes |
per |
T11247 |
320-324 |
NN |
denotes |
lane |
T11248 |
325-329 |
IN |
denotes |
from |
T11249 |
330-334 |
CD |
denotes |
four |
T11251 |
335-344 |
JJ |
denotes |
different |
T11250 |
345-351 |
NNS |
denotes |
stages |
T11252 |
352-354 |
IN |
denotes |
of |
T11253 |
355-360 |
NN |
denotes |
mouse |
T11254 |
361-372 |
NN |
denotes |
development |
T11255 |
372-373 |
. |
denotes |
. |
T11256 |
373-434 |
sentence |
denotes |
RNA from ES cells was isolated with the RNeasy kit (Qiagen). |
T11257 |
374-377 |
NN |
denotes |
RNA |
T11259 |
378-382 |
IN |
denotes |
from |
T11260 |
383-385 |
NN |
denotes |
ES |
T11261 |
386-391 |
NNS |
denotes |
cells |
T11262 |
392-395 |
VBD |
denotes |
was |
T11258 |
396-404 |
VBN |
denotes |
isolated |
T11263 |
405-409 |
IN |
denotes |
with |
T11264 |
410-413 |
DT |
denotes |
the |
T11266 |
414-420 |
NNP |
denotes |
RNeasy |
T11265 |
421-424 |
NN |
denotes |
kit |
T11267 |
425-426 |
-LRB- |
denotes |
( |
T11268 |
426-432 |
NNP |
denotes |
Qiagen |
T11269 |
432-433 |
-RRB- |
denotes |
) |
T11270 |
433-434 |
. |
denotes |
. |
T11271 |
434-654 |
sentence |
denotes |
The membranes were hybridized with a random-primed [α-32P]-dATP-labelled full length annexin A7 cDNA probe and with a β-actin specific probe for control in ExpressHyb solution according to the manufacturer's directions. |
T11272 |
435-438 |
DT |
denotes |
The |
T11273 |
439-448 |
NNS |
denotes |
membranes |
T11275 |
449-453 |
VBD |
denotes |
were |
T11274 |
454-464 |
VBN |
denotes |
hybridized |
T11276 |
465-469 |
IN |
denotes |
with |
T11277 |
470-471 |
DT |
denotes |
a |
T11279 |
472-478 |
JJ |
denotes |
random |
T11281 |
478-479 |
HYPH |
denotes |
- |
T11280 |
479-485 |
VBN |
denotes |
primed |
T11282 |
486-487 |
-LRB- |
denotes |
[ |
T11283 |
487-488 |
NN |
denotes |
α |
T11284 |
488-489 |
HYPH |
denotes |
- |
T11285 |
489-492 |
CD |
denotes |
32P |
T11286 |
492-493 |
-RRB- |
denotes |
] |
T11287 |
493-494 |
HYPH |
denotes |
- |
T11288 |
494-498 |
NN |
denotes |
dATP |
T11290 |
498-499 |
HYPH |
denotes |
- |
T11289 |
499-507 |
VBN |
denotes |
labelled |
T11291 |
508-512 |
JJ |
denotes |
full |
T11292 |
513-519 |
NN |
denotes |
length |
T11293 |
520-527 |
NN |
denotes |
annexin |
T11294 |
528-530 |
NN |
denotes |
A7 |
T11295 |
531-535 |
NN |
denotes |
cDNA |
T11278 |
536-541 |
NN |
denotes |
probe |
T11296 |
542-545 |
CC |
denotes |
and |
T11297 |
546-550 |
IN |
denotes |
with |
T11298 |
551-552 |
DT |
denotes |
a |
T11300 |
553-554 |
NN |
denotes |
β |
T11302 |
554-555 |
HYPH |
denotes |
- |
T11301 |
555-560 |
NN |
denotes |
actin |
T11303 |
561-569 |
JJ |
denotes |
specific |
T11299 |
570-575 |
NN |
denotes |
probe |
T11304 |
576-579 |
IN |
denotes |
for |
T11305 |
580-587 |
NN |
denotes |
control |
T11306 |
588-590 |
IN |
denotes |
in |
T11307 |
591-601 |
NN |
denotes |
ExpressHyb |
T11308 |
602-610 |
NN |
denotes |
solution |
T11309 |
611-620 |
VBG |
denotes |
according |
T11310 |
621-623 |
IN |
denotes |
to |
T11311 |
624-627 |
DT |
denotes |
the |
T11312 |
628-640 |
NN |
denotes |
manufacturer |
T11314 |
640-642 |
POS |
denotes |
's |
T11313 |
643-653 |
NNS |
denotes |
directions |
T11315 |
653-654 |
. |
denotes |
. |
R5436 |
T11182 |
T11183 |
nmod |
Embryo,Blot |
R5437 |
T11184 |
T11185 |
amod |
Multiple,Tissue |
R5438 |
T11185 |
T11183 |
compound |
Tissue,Blot |
R5439 |
T11186 |
T11183 |
compound |
Northern,Blot |
R5440 |
T11188 |
T11189 |
det |
The,presence |
R5441 |
T11189 |
T11190 |
nsubjpass |
presence,analyzed |
R5442 |
T11191 |
T11189 |
prep |
of,presence |
R5443 |
T11192 |
T11193 |
compound |
Annexin,A7 |
R5444 |
T11193 |
T11194 |
compound |
A7,mRNA |
R5445 |
T11194 |
T11191 |
pobj |
mRNA,of |
R5446 |
T11195 |
T11189 |
prep |
in,presence |
R5447 |
T11196 |
T11197 |
compound |
mouse,cells |
R5448 |
T11197 |
T11195 |
pobj |
cells,in |
R5449 |
T11198 |
T11197 |
compound |
ES,cells |
R5450 |
T11199 |
T11197 |
cc |
and,cells |
R5451 |
T11200 |
T11201 |
compound |
embryos,E7 |
R5452 |
T11201 |
T11197 |
conj |
E7,cells |
R5453 |
T11202 |
T11201 |
punct |
", ",E7 |
R5454 |
T11203 |
T11201 |
conj |
E11,E7 |
R5455 |
T11204 |
T11203 |
punct |
", ",E11 |
R5456 |
T11205 |
T11203 |
conj |
E15,E11 |
R5457 |
T11206 |
T11205 |
cc |
and,E15 |
R5458 |
T11207 |
T11205 |
conj |
E17,E15 |
R5459 |
T11208 |
T11190 |
auxpass |
was,analyzed |
R5460 |
T11209 |
T11190 |
prep |
by,analyzed |
R5461 |
T11210 |
T11211 |
compound |
northern,blot |
R5462 |
T11211 |
T11212 |
compound |
blot,analysis |
R5463 |
T11212 |
T11209 |
pobj |
analysis,by |
R5464 |
T11213 |
T11190 |
punct |
.,analyzed |
R5465 |
T11215 |
T11216 |
det |
An,membrane |
R5466 |
T11216 |
T11222 |
nsubjpass |
membrane,obtained |
R5467 |
T11217 |
T11216 |
nmod |
Embryo,membrane |
R5468 |
T11218 |
T11219 |
amod |
Multiple,Tissue |
R5469 |
T11219 |
T11216 |
compound |
Tissue,membrane |
R5470 |
T11220 |
T11221 |
compound |
Northern,Blot |
R5471 |
T11221 |
T11216 |
compound |
Blot,membrane |
R5472 |
T11223 |
T11222 |
auxpass |
was,obtained |
R5473 |
T11224 |
T11222 |
prep |
from,obtained |
R5474 |
T11225 |
T11226 |
compound |
Clontech,Laboratories |
R5475 |
T11226 |
T11224 |
pobj |
Laboratories,from |
R5476 |
T11227 |
T11228 |
punct |
(,Heidelberg |
R5477 |
T11228 |
T11226 |
parataxis |
Heidelberg,Laboratories |
R5478 |
T11229 |
T11228 |
punct |
", ",Heidelberg |
R5479 |
T11230 |
T11228 |
npadvmod |
FRG,Heidelberg |
R5480 |
T11231 |
T11228 |
punct |
),Heidelberg |
R5481 |
T11232 |
T11222 |
punct |
.,obtained |
R5482 |
T11234 |
T11235 |
nsubj |
It,contained |
R5483 |
T11236 |
T11237 |
advmod |
approximately,2 |
R5484 |
T11237 |
T11238 |
nummod |
2,μg |
R5485 |
T11238 |
T11235 |
dobj |
μg,contained |
R5486 |
T11239 |
T11238 |
prep |
of,μg |
R5487 |
T11240 |
T11241 |
nmod |
poly,RNA |
R5488 |
T11241 |
T11239 |
pobj |
RNA,of |
R5489 |
T11242 |
T11243 |
punct |
(,A |
R5490 |
T11243 |
T11241 |
parataxis |
A,RNA |
R5491 |
T11244 |
T11243 |
punct |
),A |
R5492 |
T11245 |
T11241 |
punct |
+,RNA |
R5493 |
T11246 |
T11238 |
prep |
per,μg |
R5494 |
T11247 |
T11246 |
pobj |
lane,per |
R5495 |
T11248 |
T11238 |
prep |
from,μg |
R5496 |
T11249 |
T11250 |
nummod |
four,stages |
R5497 |
T11250 |
T11248 |
pobj |
stages,from |
R5498 |
T11251 |
T11250 |
amod |
different,stages |
R5499 |
T11252 |
T11250 |
prep |
of,stages |
R5500 |
T11253 |
T11254 |
compound |
mouse,development |
R5501 |
T11254 |
T11252 |
pobj |
development,of |
R5502 |
T11255 |
T11235 |
punct |
.,contained |
R5503 |
T11257 |
T11258 |
nsubjpass |
RNA,isolated |
R5504 |
T11259 |
T11257 |
prep |
from,RNA |
R5505 |
T11260 |
T11261 |
compound |
ES,cells |
R5506 |
T11261 |
T11259 |
pobj |
cells,from |
R5507 |
T11262 |
T11258 |
auxpass |
was,isolated |
R5508 |
T11263 |
T11258 |
prep |
with,isolated |
R5509 |
T11264 |
T11265 |
det |
the,kit |
R5510 |
T11265 |
T11263 |
pobj |
kit,with |
R5511 |
T11266 |
T11265 |
compound |
RNeasy,kit |
R5512 |
T11267 |
T11268 |
punct |
(,Qiagen |
R5513 |
T11268 |
T11265 |
parataxis |
Qiagen,kit |
R5514 |
T11269 |
T11268 |
punct |
),Qiagen |
R5515 |
T11270 |
T11258 |
punct |
.,isolated |
R5516 |
T11272 |
T11273 |
det |
The,membranes |
R5517 |
T11273 |
T11274 |
nsubjpass |
membranes,hybridized |
R5518 |
T11275 |
T11274 |
auxpass |
were,hybridized |
R5519 |
T11276 |
T11274 |
prep |
with,hybridized |
R5520 |
T11277 |
T11278 |
det |
a,probe |
R5521 |
T11278 |
T11276 |
pobj |
probe,with |
R5522 |
T11279 |
T11280 |
amod |
random,primed |
R5523 |
T11280 |
T11278 |
amod |
primed,probe |
R5524 |
T11281 |
T11280 |
punct |
-,primed |
R5525 |
T11282 |
T11283 |
punct |
[,α |
R5526 |
T11283 |
T11280 |
parataxis |
α,primed |
R5527 |
T11284 |
T11283 |
punct |
-,α |
R5528 |
T11285 |
T11283 |
nummod |
32P,α |
R5529 |
T11286 |
T11283 |
punct |
],α |
R5530 |
T11287 |
T11278 |
punct |
-,probe |
R5531 |
T11288 |
T11289 |
npadvmod |
dATP,labelled |
R5532 |
T11289 |
T11278 |
amod |
labelled,probe |
R5533 |
T11290 |
T11289 |
punct |
-,labelled |
R5534 |
T11291 |
T11292 |
amod |
full,length |
R5535 |
T11292 |
T11278 |
compound |
length,probe |
R5536 |
T11293 |
T11294 |
compound |
annexin,A7 |
R5537 |
T11294 |
T11278 |
compound |
A7,probe |
R5538 |
T11295 |
T11278 |
compound |
cDNA,probe |
R5539 |
T11296 |
T11276 |
cc |
and,with |
R5540 |
T11297 |
T11276 |
conj |
with,with |
R5541 |
T11298 |
T11299 |
det |
a,probe |
R5542 |
T11299 |
T11297 |
pobj |
probe,with |
R5543 |
T11300 |
T11301 |
compound |
β,actin |
R5544 |
T11301 |
T11303 |
npadvmod |
actin,specific |
R5545 |
T11302 |
T11301 |
punct |
-,actin |
R5546 |
T11303 |
T11299 |
amod |
specific,probe |
R5547 |
T11304 |
T11274 |
prep |
for,hybridized |
R5548 |
T11305 |
T11304 |
pobj |
control,for |
R5549 |
T11306 |
T11274 |
prep |
in,hybridized |
R5550 |
T11307 |
T11308 |
compound |
ExpressHyb,solution |
R5551 |
T11308 |
T11306 |
pobj |
solution,in |
R5552 |
T11309 |
T11274 |
prep |
according,hybridized |
R5553 |
T11310 |
T11309 |
prep |
to,according |
R5554 |
T11311 |
T11312 |
det |
the,manufacturer |
R5555 |
T11312 |
T11313 |
poss |
manufacturer,directions |
R5556 |
T11313 |
T11310 |
pobj |
directions,to |
R5557 |
T11314 |
T11312 |
case |
's,manufacturer |
R5558 |
T11315 |
T11274 |
punct |
.,hybridized |