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PMC:1087847 / 33737-35575 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T9606 0-18 NN denotes Immunofluorescence
T9607 19-29 NN denotes microscopy
T9608 29-263 sentence denotes Immunohistochemical staining using mAb 203–217 as the primary antibody was with AlexaFluor488-conjugated goat anti-mouse IgG (Molecular Probes, Leiden, The Nether-lands) or Cy3-labeled goat anti-mouse IgG as the secondary antibodies.
T9609 30-49 JJ denotes Immunohistochemical
T9610 50-58 NN denotes staining
T9612 59-64 VBG denotes using
T9613 65-68 NN denotes mAb
T9614 69-72 CD denotes 203
T9615 72-73 SYM denotes –
T9616 73-76 CD denotes 217
T9617 77-79 IN denotes as
T9618 80-83 DT denotes the
T9620 84-91 JJ denotes primary
T9619 92-100 NN denotes antibody
T9611 101-104 VBD denotes was
T9621 105-109 IN denotes with
T9622 110-123 NN denotes AlexaFluor488
T9624 123-124 HYPH denotes -
T9623 124-134 VBN denotes conjugated
T9626 135-139 NN denotes goat
T9627 140-150 JJ denotes anti-mouse
T9625 151-154 NN denotes IgG
T9628 155-156 -LRB- denotes (
T9630 156-165 NNP denotes Molecular
T9629 166-172 NNP denotes Probes
T9631 172-174 , denotes ,
T9632 174-180 NNP denotes Leiden
T9633 180-182 , denotes ,
T9634 182-185 DT denotes The
T9636 186-192 NNP denotes Nether
T9637 192-193 HYPH denotes -
T9635 193-198 NNP denotes lands
T9638 198-199 -RRB- denotes )
T9639 200-202 CC denotes or
T9640 203-206 NN denotes Cy3
T9642 206-207 HYPH denotes -
T9641 207-214 VBN denotes labeled
T9644 215-219 NN denotes goat
T9645 220-230 JJ denotes anti-mouse
T9643 231-234 NN denotes IgG
T9646 235-237 IN denotes as
T9647 238-241 DT denotes the
T9649 242-251 JJ denotes secondary
T9648 252-262 NNS denotes antibodies
T9650 262-263 . denotes .
T9651 263-409 sentence denotes Polyclonal anti-Annexin A7 antibody and anti-GFAP-antibody were combined with Alexa Fluor 568-conjugated goat anti-rabbit IgG (Molecular Probes).
T9652 264-274 JJ denotes Polyclonal
T9654 275-287 JJ denotes anti-Annexin
T9655 288-290 NN denotes A7
T9653 291-299 NN denotes antibody
T9657 300-303 CC denotes and
T9658 304-313 JJ denotes anti-GFAP
T9660 313-314 HYPH denotes -
T9659 314-322 NN denotes antibody
T9661 323-327 VBD denotes were
T9656 328-336 VBN denotes combined
T9662 337-341 IN denotes with
T9663 342-347 NNP denotes Alexa
T9664 348-353 NNP denotes Fluor
T9666 354-357 CD denotes 568
T9667 357-358 HYPH denotes -
T9665 358-368 VBN denotes conjugated
T9669 369-373 NN denotes goat
T9670 374-385 JJ denotes anti-rabbit
T9668 386-389 NN denotes IgG
T9671 390-391 -LRB- denotes (
T9673 391-400 NNP denotes Molecular
T9672 401-407 NNP denotes Probes
T9674 407-408 -RRB- denotes )
T9675 408-409 . denotes .
T9676 409-612 sentence denotes The specific labelling of Annexin A7 detected with the anti-mouse-IgG secondary antibodies in mouse tissue sections was confirmed using primary antibodies, which were directly coupled to Alexa Fluor488.
T9677 410-413 DT denotes The
T9679 414-422 JJ denotes specific
T9678 423-432 NN denotes labelling
T9681 433-435 IN denotes of
T9682 436-443 NN denotes Annexin
T9683 444-446 NN denotes A7
T9684 447-455 VBN denotes detected
T9685 456-460 IN denotes with
T9686 461-464 DT denotes the
T9688 465-475 JJ denotes anti-mouse
T9690 475-476 HYPH denotes -
T9689 476-479 NN denotes IgG
T9691 480-489 JJ denotes secondary
T9687 490-500 NNS denotes antibodies
T9692 501-503 IN denotes in
T9693 504-509 NN denotes mouse
T9695 510-516 NN denotes tissue
T9694 517-525 NNS denotes sections
T9696 526-529 VBD denotes was
T9680 530-539 VBN denotes confirmed
T9697 540-545 VBG denotes using
T9698 546-553 JJ denotes primary
T9699 554-564 NNS denotes antibodies
T9700 564-566 , denotes ,
T9701 566-571 WDT denotes which
T9703 572-576 VBD denotes were
T9704 577-585 RB denotes directly
T9702 586-593 VBN denotes coupled
T9705 594-596 IN denotes to
T9706 597-602 NNP denotes Alexa
T9707 603-611 NN denotes Fluor488
T9708 611-612 . denotes .
T9709 612-816 sentence denotes The paraffin-embedded sections were first treated three times for 2 min in xylene to remove paraffin and then rehydrated step by step with descending concentrations of ethanol for antibody staining [35].
T9710 613-616 DT denotes The
T9712 617-625 NN denotes paraffin
T9714 625-626 HYPH denotes -
T9713 626-634 VBN denotes embedded
T9711 635-643 NNS denotes sections
T9716 644-648 VBD denotes were
T9717 649-654 RB denotes first
T9715 655-662 VBN denotes treated
T9718 663-668 CD denotes three
T9719 669-674 NNS denotes times
T9720 675-678 IN denotes for
T9721 679-680 CD denotes 2
T9722 681-684 NN denotes min
T9723 685-687 IN denotes in
T9724 688-694 NN denotes xylene
T9725 695-697 TO denotes to
T9726 698-704 VB denotes remove
T9727 705-713 NN denotes paraffin
T9728 714-717 CC denotes and
T9729 718-722 RB denotes then
T9730 723-733 VBD denotes rehydrated
T9731 734-738 NN denotes step
T9732 739-741 IN denotes by
T9733 742-746 NN denotes step
T9734 747-751 IN denotes with
T9735 752-762 VBG denotes descending
T9736 763-777 NNS denotes concentrations
T9737 778-780 IN denotes of
T9738 781-788 NN denotes ethanol
T9739 789-792 IN denotes for
T9740 793-801 NN denotes antibody
T9741 802-810 NN denotes staining
T9742 811-812 -LRB- denotes [
T9743 812-814 CD denotes 35
T9744 814-815 -RRB- denotes ]
T9745 815-816 . denotes .
T9746 816-1058 sentence denotes After three washings in PBS (137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.4), the tissue sections were incubated in PBS containing 0.1% trypsin for 10 min at room temperature to free formaldehyde-crosslinked epitopes [15-17].
T9747 817-822 IN denotes After
T9749 823-828 CD denotes three
T9750 829-837 NNS denotes washings
T9751 838-840 IN denotes in
T9752 841-844 NN denotes PBS
T9753 845-846 -LRB- denotes (
T9755 846-849 CD denotes 137
T9756 850-852 NN denotes mM
T9754 853-857 NN denotes NaCl
T9757 857-859 , denotes ,
T9758 859-862 CD denotes 2.7
T9759 863-865 NN denotes mM
T9760 866-869 NN denotes KCl
T9761 869-871 , denotes ,
T9762 871-874 CD denotes 8.1
T9763 875-877 NN denotes mM
T9764 878-885 NN denotes Na2HPO4
T9765 885-887 , denotes ,
T9766 887-890 CD denotes 1.5
T9767 891-893 NN denotes mM
T9768 894-900 NN denotes KH2PO4
T9769 900-902 , denotes ,
T9770 902-904 NN denotes pH
T9771 905-908 CD denotes 7.4
T9772 908-909 -RRB- denotes )
T9773 909-911 , denotes ,
T9774 911-914 DT denotes the
T9776 915-921 NN denotes tissue
T9775 922-930 NNS denotes sections
T9777 931-935 VBD denotes were
T9748 936-945 VBN denotes incubated
T9778 946-948 IN denotes in
T9779 949-952 NN denotes PBS
T9780 953-963 VBG denotes containing
T9781 964-967 CD denotes 0.1
T9782 967-968 NN denotes %
T9783 969-976 NN denotes trypsin
T9784 977-980 IN denotes for
T9785 981-983 CD denotes 10
T9786 984-987 NN denotes min
T9787 988-990 IN denotes at
T9788 991-995 NN denotes room
T9789 996-1007 NN denotes temperature
T9790 1008-1010 TO denotes to
T9791 1011-1015 VB denotes free
T9792 1016-1028 NN denotes formaldehyde
T9794 1028-1029 HYPH denotes -
T9793 1029-1040 VBN denotes crosslinked
T9795 1041-1049 NNS denotes epitopes
T9796 1050-1051 -LRB- denotes [
T9797 1051-1053 CD denotes 15
T9798 1053-1054 SYM denotes -
T9799 1054-1056 CD denotes 17
T9800 1056-1057 -RRB- denotes ]
T9801 1057-1058 . denotes .
T9802 1058-1254 sentence denotes After additional three washes with PBS, non-specific binding sites were blocked by incubating the slides with PBG (0.5 % BSA, 0.045 % fish gelatine in PBS) in 10% normal goat serum (NGS) for 2 h.
T9803 1059-1064 IN denotes After
T9805 1065-1075 JJ denotes additional
T9807 1076-1081 CD denotes three
T9806 1082-1088 NNS denotes washes
T9808 1089-1093 IN denotes with
T9809 1094-1097 NN denotes PBS
T9810 1097-1099 , denotes ,
T9811 1099-1111 JJ denotes non-specific
T9813 1112-1119 NN denotes binding
T9812 1120-1125 NNS denotes sites
T9814 1126-1130 VBD denotes were
T9804 1131-1138 VBN denotes blocked
T9815 1139-1141 IN denotes by
T9816 1142-1152 VBG denotes incubating
T9817 1153-1156 DT denotes the
T9818 1157-1163 NNS denotes slides
T9819 1164-1168 IN denotes with
T9820 1169-1172 NN denotes PBG
T9821 1173-1174 -LRB- denotes (
T9823 1174-1177 CD denotes 0.5
T9824 1178-1179 NN denotes %
T9822 1180-1183 NN denotes BSA
T9825 1183-1185 , denotes ,
T9826 1185-1190 CD denotes 0.045
T9827 1191-1192 NN denotes %
T9829 1193-1197 NN denotes fish
T9828 1198-1206 NN denotes gelatine
T9830 1207-1209 IN denotes in
T9831 1210-1213 NN denotes PBS
T9832 1213-1214 -RRB- denotes )
T9833 1215-1217 IN denotes in
T9834 1218-1220 CD denotes 10
T9835 1220-1221 NN denotes %
T9836 1222-1228 JJ denotes normal
T9838 1229-1233 NN denotes goat
T9837 1234-1239 NN denotes serum
T9839 1240-1241 -LRB- denotes (
T9840 1241-1244 NN denotes NGS
T9841 1244-1245 -RRB- denotes )
T9842 1246-1249 IN denotes for
T9843 1250-1251 CD denotes 2
T9844 1252-1253 NN denotes h
T9845 1253-1254 . denotes .
T9846 1254-1366 sentence denotes Incubation with the primary antibodies was performed at 4°C overnight diluted in PBG supplemented with 1 % NGS.
T9847 1255-1265 NN denotes Incubation
T9849 1266-1270 IN denotes with
T9850 1271-1274 DT denotes the
T9852 1275-1282 JJ denotes primary
T9851 1283-1293 NNS denotes antibodies
T9853 1294-1297 VBD denotes was
T9854 1298-1307 VBN denotes performed
T9855 1308-1310 IN denotes at
T9856 1311-1312 CD denotes 4
T9857 1312-1314 NN denotes °C
T9858 1315-1324 RB denotes overnight
T9848 1325-1332 VBN denotes diluted
T9859 1333-1335 IN denotes in
T9860 1336-1339 NN denotes PBG
T9861 1340-1352 VBN denotes supplemented
T9862 1353-1357 IN denotes with
T9863 1358-1359 CD denotes 1
T9864 1360-1361 NN denotes %
T9865 1362-1365 NN denotes NGS
T9866 1365-1366 . denotes .
T9867 1366-1599 sentence denotes After five washes in PBG for 5 min each, the slides were incubated for 1 h with the secondary antibody at room temperature, washed three times in PBG and three times in PBS, 5 min each, rinsed in water and embedded in gelvatol [36].
T9868 1367-1372 IN denotes After
T9870 1373-1377 CD denotes five
T9871 1378-1384 NNS denotes washes
T9872 1385-1387 IN denotes in
T9873 1388-1391 NN denotes PBG
T9874 1392-1395 IN denotes for
T9875 1396-1397 CD denotes 5
T9876 1398-1401 NN denotes min
T9877 1402-1406 DT denotes each
T9878 1406-1408 , denotes ,
T9879 1408-1411 DT denotes the
T9880 1412-1418 NNS denotes slides
T9881 1419-1423 VBD denotes were
T9869 1424-1433 VBN denotes incubated
T9882 1434-1437 IN denotes for
T9883 1438-1439 CD denotes 1
T9884 1440-1441 NN denotes h
T9885 1442-1446 IN denotes with
T9886 1447-1450 DT denotes the
T9888 1451-1460 JJ denotes secondary
T9887 1461-1469 NN denotes antibody
T9889 1470-1472 IN denotes at
T9890 1473-1477 NN denotes room
T9891 1478-1489 NN denotes temperature
T9892 1489-1491 , denotes ,
T9893 1491-1497 VBD denotes washed
T9894 1498-1503 CD denotes three
T9895 1504-1509 NNS denotes times
T9896 1510-1512 IN denotes in
T9897 1513-1516 NN denotes PBG
T9898 1517-1520 CC denotes and
T9899 1521-1526 CD denotes three
T9900 1527-1532 NNS denotes times
T9901 1533-1535 IN denotes in
T9902 1536-1539 NN denotes PBS
T9903 1539-1541 , denotes ,
T9904 1541-1542 CD denotes 5
T9905 1543-1546 NNS denotes min
T9906 1547-1551 DT denotes each
T9907 1551-1553 , denotes ,
T9908 1553-1559 VBN denotes rinsed
T9909 1560-1562 IN denotes in
T9910 1563-1568 NN denotes water
T9911 1569-1572 CC denotes and
T9912 1573-1581 VBN denotes embedded
T9913 1582-1584 IN denotes in
T9914 1585-1593 NN denotes gelvatol
T9915 1594-1595 -LRB- denotes [
T9916 1595-1597 CD denotes 36
T9917 1597-1598 -RRB- denotes ]
T9918 1598-1599 . denotes .
T9919 1599-1740 sentence denotes For control, sections were incubated only with the secondary antibody or sections obtained from an annexin A7 knockout mouse were used [10].
T9920 1600-1603 IN denotes For
T9922 1604-1611 NN denotes control
T9923 1611-1613 , denotes ,
T9924 1613-1621 NNS denotes sections
T9925 1622-1626 VBD denotes were
T9921 1627-1636 VBN denotes incubated
T9926 1637-1641 RB denotes only
T9927 1642-1646 IN denotes with
T9928 1647-1650 DT denotes the
T9930 1651-1660 JJ denotes secondary
T9929 1661-1669 NN denotes antibody
T9931 1670-1672 CC denotes or
T9932 1673-1681 NNS denotes sections
T9934 1682-1690 VBN denotes obtained
T9935 1691-1695 IN denotes from
T9936 1696-1698 DT denotes an
T9938 1699-1706 NN denotes annexin
T9939 1707-1709 NN denotes A7
T9940 1710-1718 NN denotes knockout
T9937 1719-1724 NN denotes mouse
T9941 1725-1729 VBD denotes were
T9933 1730-1734 VBN denotes used
T9942 1735-1736 -LRB- denotes [
T9943 1736-1738 CD denotes 10
T9944 1738-1739 -RRB- denotes ]
T9945 1739-1740 . denotes .
T9946 1740-1838 sentence denotes Samples were examined with a confocal laser scan microscope (Leica TCS SP; Leica, Bensheim, FRG).
T9947 1741-1748 NNS denotes Samples
T9949 1749-1753 VBD denotes were
T9948 1754-1762 VBN denotes examined
T9950 1763-1767 IN denotes with
T9951 1768-1769 DT denotes a
T9953 1770-1778 JJ denotes confocal
T9954 1779-1784 NN denotes laser
T9955 1785-1789 NN denotes scan
T9952 1790-1800 NN denotes microscope
T9956 1801-1802 -LRB- denotes (
T9958 1802-1807 NNP denotes Leica
T9959 1808-1811 NN denotes TCS
T9957 1812-1814 NN denotes SP
T9960 1814-1815 : denotes ;
T9961 1816-1821 NNP denotes Leica
T9962 1821-1823 , denotes ,
T9963 1823-1831 NNP denotes Bensheim
T9964 1831-1833 , denotes ,
T9965 1833-1836 NNP denotes FRG
T9966 1836-1837 -RRB- denotes )
T9967 1837-1838 . denotes .
R4554 T9606 T9607 compound Immunofluorescence,microscopy
R4555 T9609 T9610 amod Immunohistochemical,staining
R4556 T9610 T9611 nsubj staining,was
R4557 T9612 T9610 acl using,staining
R4558 T9613 T9614 nmod mAb,203
R4559 T9614 T9612 dobj 203,using
R4560 T9615 T9616 punct –,217
R4561 T9616 T9614 prep 217,203
R4562 T9617 T9612 prep as,using
R4563 T9618 T9619 det the,antibody
R4564 T9619 T9617 pobj antibody,as
R4565 T9620 T9619 amod primary,antibody
R4566 T9621 T9611 prep with,was
R4567 T9622 T9623 npadvmod AlexaFluor488,conjugated
R4568 T9623 T9625 amod conjugated,IgG
R4569 T9624 T9623 punct -,conjugated
R4570 T9625 T9621 pobj IgG,with
R4571 T9626 T9625 nmod goat,IgG
R4572 T9627 T9625 amod anti-mouse,IgG
R4573 T9628 T9629 punct (,Probes
R4574 T9629 T9625 parataxis Probes,IgG
R4575 T9630 T9629 compound Molecular,Probes
R4576 T9631 T9629 punct ", ",Probes
R4577 T9632 T9629 npadvmod Leiden,Probes
R4578 T9633 T9629 punct ", ",Probes
R4579 T9634 T9635 det The,lands
R4580 T9635 T9629 npadvmod lands,Probes
R4581 T9636 T9635 compound Nether,lands
R4582 T9637 T9635 punct -,lands
R4583 T9638 T9629 punct ),Probes
R4584 T9639 T9625 cc or,IgG
R4585 T9640 T9641 npadvmod Cy3,labeled
R4586 T9641 T9643 amod labeled,IgG
R4587 T9642 T9641 punct -,labeled
R4588 T9643 T9625 conj IgG,IgG
R4589 T9644 T9643 nmod goat,IgG
R4590 T9645 T9643 amod anti-mouse,IgG
R4591 T9646 T9625 prep as,IgG
R4592 T9647 T9648 det the,antibodies
R4593 T9648 T9646 pobj antibodies,as
R4594 T9649 T9648 amod secondary,antibodies
R4595 T9650 T9611 punct .,was
R4596 T9652 T9653 amod Polyclonal,antibody
R4597 T9653 T9656 nsubjpass antibody,combined
R4598 T9654 T9655 amod anti-Annexin,A7
R4599 T9655 T9653 compound A7,antibody
R4600 T9657 T9653 cc and,antibody
R4601 T9658 T9659 nmod anti-GFAP,antibody
R4602 T9659 T9653 conj antibody,antibody
R4603 T9660 T9659 punct -,antibody
R4604 T9661 T9656 auxpass were,combined
R4605 T9662 T9656 prep with,combined
R4606 T9663 T9664 compound Alexa,Fluor
R4607 T9664 T9665 npadvmod Fluor,conjugated
R4608 T9665 T9668 amod conjugated,IgG
R4609 T9666 T9664 nummod 568,Fluor
R4610 T9667 T9665 punct -,conjugated
R4611 T9668 T9662 pobj IgG,with
R4612 T9669 T9668 nmod goat,IgG
R4613 T9670 T9668 amod anti-rabbit,IgG
R4614 T9671 T9672 punct (,Probes
R4615 T9672 T9668 parataxis Probes,IgG
R4616 T9673 T9672 compound Molecular,Probes
R4617 T9674 T9672 punct ),Probes
R4618 T9675 T9656 punct .,combined
R4619 T9677 T9678 det The,labelling
R4620 T9678 T9680 nsubjpass labelling,confirmed
R4621 T9679 T9678 amod specific,labelling
R4622 T9681 T9678 prep of,labelling
R4623 T9682 T9683 compound Annexin,A7
R4624 T9683 T9681 pobj A7,of
R4625 T9684 T9678 acl detected,labelling
R4626 T9685 T9684 prep with,detected
R4627 T9686 T9687 det the,antibodies
R4628 T9687 T9685 pobj antibodies,with
R4629 T9688 T9689 amod anti-mouse,IgG
R4630 T9689 T9687 nmod IgG,antibodies
R4631 T9690 T9689 punct -,IgG
R4632 T9691 T9687 amod secondary,antibodies
R4633 T9692 T9684 prep in,detected
R4634 T9693 T9694 compound mouse,sections
R4635 T9694 T9692 pobj sections,in
R4636 T9695 T9694 compound tissue,sections
R4637 T9696 T9680 auxpass was,confirmed
R4638 T9697 T9680 advcl using,confirmed
R4639 T9698 T9699 amod primary,antibodies
R4640 T9699 T9697 dobj antibodies,using
R4641 T9700 T9699 punct ", ",antibodies
R4642 T9701 T9702 dep which,coupled
R4643 T9702 T9699 relcl coupled,antibodies
R4644 T9703 T9702 auxpass were,coupled
R4645 T9704 T9702 advmod directly,coupled
R4646 T9705 T9702 prep to,coupled
R4647 T9706 T9707 compound Alexa,Fluor488
R4648 T9707 T9705 pobj Fluor488,to
R4649 T9708 T9680 punct .,confirmed
R4650 T9710 T9711 det The,sections
R4651 T9711 T9715 nsubjpass sections,treated
R4652 T9712 T9713 npadvmod paraffin,embedded
R4653 T9713 T9711 amod embedded,sections
R4654 T9714 T9713 punct -,embedded
R4655 T9716 T9715 auxpass were,treated
R4656 T9717 T9715 advmod first,treated
R4657 T9718 T9719 nummod three,times
R4658 T9719 T9715 npadvmod times,treated
R4659 T9720 T9715 prep for,treated
R4660 T9721 T9722 nummod 2,min
R4661 T9722 T9720 pobj min,for
R4662 T9723 T9715 prep in,treated
R4663 T9724 T9723 pobj xylene,in
R4664 T9725 T9726 aux to,remove
R4665 T9726 T9715 advcl remove,treated
R4666 T9727 T9726 dobj paraffin,remove
R4667 T9728 T9715 cc and,treated
R4668 T9729 T9730 advmod then,rehydrated
R4669 T9730 T9715 conj rehydrated,treated
R4670 T9731 T9730 npadvmod step,rehydrated
R4671 T9732 T9731 prep by,step
R4672 T9733 T9732 pobj step,by
R4673 T9734 T9730 prep with,rehydrated
R4674 T9735 T9736 amod descending,concentrations
R4675 T9736 T9734 pobj concentrations,with
R4676 T9737 T9736 prep of,concentrations
R4677 T9738 T9737 pobj ethanol,of
R4678 T9739 T9730 prep for,rehydrated
R4679 T9740 T9741 compound antibody,staining
R4680 T9741 T9739 pobj staining,for
R4681 T9742 T9743 punct [,35
R4682 T9743 T9730 parataxis 35,rehydrated
R4683 T9744 T9743 punct ],35
R4684 T9745 T9715 punct .,treated
R4685 T9747 T9748 prep After,incubated
R4686 T9749 T9750 nummod three,washings
R4687 T9750 T9747 pobj washings,After
R4688 T9751 T9750 prep in,washings
R4689 T9752 T9751 pobj PBS,in
R4690 T9753 T9754 punct (,NaCl
R4691 T9754 T9750 parataxis NaCl,washings
R4692 T9755 T9756 nummod 137,mM
R4693 T9756 T9754 compound mM,NaCl
R4694 T9757 T9754 punct ", ",NaCl
R4695 T9758 T9759 nummod 2.7,mM
R4696 T9759 T9760 compound mM,KCl
R4697 T9760 T9754 appos KCl,NaCl
R4698 T9761 T9754 punct ", ",NaCl
R4699 T9762 T9763 nummod 8.1,mM
R4700 T9763 T9764 compound mM,Na2HPO4
R4701 T9764 T9754 appos Na2HPO4,NaCl
R4702 T9765 T9754 punct ", ",NaCl
R4703 T9766 T9767 nummod 1.5,mM
R4704 T9767 T9768 compound mM,KH2PO4
R4705 T9768 T9754 appos KH2PO4,NaCl
R4706 T9769 T9754 punct ", ",NaCl
R4707 T9770 T9754 npadvmod pH,NaCl
R4708 T9771 T9770 nummod 7.4,pH
R4709 T9772 T9754 punct ),NaCl
R4710 T9773 T9748 punct ", ",incubated
R4711 T9774 T9775 det the,sections
R4712 T9775 T9748 nsubjpass sections,incubated
R4713 T9776 T9775 compound tissue,sections
R4714 T9777 T9748 auxpass were,incubated
R4715 T9778 T9748 prep in,incubated
R4716 T9779 T9778 pobj PBS,in
R4717 T9780 T9779 acl containing,PBS
R4718 T9781 T9782 nummod 0.1,%
R4719 T9782 T9783 compound %,trypsin
R4720 T9783 T9780 dobj trypsin,containing
R4721 T9784 T9748 prep for,incubated
R4722 T9785 T9786 nummod 10,min
R4723 T9786 T9784 pobj min,for
R4724 T9787 T9748 prep at,incubated
R4725 T9788 T9789 compound room,temperature
R4726 T9789 T9787 pobj temperature,at
R4727 T9790 T9791 aux to,free
R4728 T9791 T9748 advcl free,incubated
R4729 T9792 T9793 npadvmod formaldehyde,crosslinked
R4730 T9793 T9795 amod crosslinked,epitopes
R4731 T9794 T9793 punct -,crosslinked
R4732 T9795 T9791 dobj epitopes,free
R4733 T9796 T9797 punct [,15
R4734 T9797 T9748 parataxis 15,incubated
R4735 T9798 T9799 punct -,17
R4736 T9799 T9797 prep 17,15
R4737 T9800 T9797 punct ],15
R4738 T9801 T9748 punct .,incubated
R4739 T9803 T9804 prep After,blocked
R4740 T9805 T9806 amod additional,washes
R4741 T9806 T9803 pobj washes,After
R4742 T9807 T9806 nummod three,washes
R4743 T9808 T9806 prep with,washes
R4744 T9809 T9808 pobj PBS,with
R4745 T9810 T9804 punct ", ",blocked
R4746 T9811 T9812 amod non-specific,sites
R4747 T9812 T9804 nsubjpass sites,blocked
R4748 T9813 T9812 compound binding,sites
R4749 T9814 T9804 auxpass were,blocked
R4750 T9815 T9804 prep by,blocked
R4751 T9816 T9815 pcomp incubating,by
R4752 T9817 T9818 det the,slides
R4753 T9818 T9816 dobj slides,incubating
R4754 T9819 T9816 prep with,incubating
R4755 T9820 T9819 pobj PBG,with
R4756 T9821 T9822 punct (,BSA
R4757 T9822 T9820 parataxis BSA,PBG
R4758 T9823 T9824 nummod 0.5,%
R4759 T9824 T9822 compound %,BSA
R4760 T9825 T9822 punct ", ",BSA
R4761 T9826 T9827 nummod 0.045,%
R4762 T9827 T9828 compound %,gelatine
R4763 T9828 T9822 appos gelatine,BSA
R4764 T9829 T9828 compound fish,gelatine
R4765 T9830 T9822 prep in,BSA
R4766 T9831 T9830 pobj PBS,in
R4767 T9832 T9822 punct ),BSA
R4768 T9833 T9816 prep in,incubating
R4769 T9834 T9835 nummod 10,%
R4770 T9835 T9833 pobj %,in
R4771 T9836 T9837 amod normal,serum
R4772 T9837 T9835 appos serum,%
R4773 T9838 T9837 compound goat,serum
R4774 T9839 T9837 punct (,serum
R4775 T9840 T9837 appos NGS,serum
R4776 T9841 T9835 punct ),%
R4777 T9842 T9816 prep for,incubating
R4778 T9843 T9844 nummod 2,h
R4779 T9844 T9842 pobj h,for
R4780 T9845 T9804 punct .,blocked
R4781 T9847 T9848 nsubjpass Incubation,diluted
R4782 T9849 T9847 prep with,Incubation
R4783 T9850 T9851 det the,antibodies
R4784 T9851 T9849 pobj antibodies,with
R4785 T9852 T9851 amod primary,antibodies
R4786 T9853 T9848 auxpass was,diluted
R4787 T9854 T9848 aux performed,diluted
R4788 T9855 T9848 prep at,diluted
R4789 T9856 T9857 nummod 4,°C
R4790 T9857 T9855 pobj °C,at
R4791 T9858 T9848 advmod overnight,diluted
R4792 T9859 T9848 prep in,diluted
R4793 T9860 T9859 pobj PBG,in
R4794 T9861 T9860 acl supplemented,PBG
R4795 T9862 T9861 prep with,supplemented
R4796 T9863 T9864 nummod 1,%
R4797 T9864 T9865 compound %,NGS
R4798 T9865 T9862 pobj NGS,with
R4799 T9866 T9848 punct .,diluted
R4800 T9868 T9869 prep After,incubated
R4801 T9870 T9871 nummod five,washes
R4802 T9871 T9868 pobj washes,After
R4803 T9872 T9871 prep in,washes
R4804 T9873 T9872 pobj PBG,in
R4805 T9874 T9871 prep for,washes
R4806 T9875 T9876 nummod 5,min
R4807 T9876 T9874 pobj min,for
R4808 T9877 T9876 appos each,min
R4809 T9878 T9869 punct ", ",incubated
R4810 T9879 T9880 det the,slides
R4811 T9880 T9869 nsubjpass slides,incubated
R4812 T9881 T9869 auxpass were,incubated
R4813 T9882 T9869 prep for,incubated
R4814 T9883 T9884 nummod 1,h
R4815 T9884 T9882 pobj h,for
R4816 T9885 T9869 prep with,incubated
R4817 T9886 T9887 det the,antibody
R4818 T9887 T9885 pobj antibody,with
R4819 T9888 T9887 amod secondary,antibody
R4820 T9889 T9869 prep at,incubated
R4821 T9890 T9891 compound room,temperature
R4822 T9891 T9889 pobj temperature,at
R4823 T9892 T9869 punct ", ",incubated
R4824 T9893 T9869 conj washed,incubated
R4825 T9894 T9895 nummod three,times
R4826 T9895 T9893 npadvmod times,washed
R4827 T9896 T9893 prep in,washed
R4828 T9897 T9896 pobj PBG,in
R4829 T9898 T9893 cc and,washed
R4830 T9899 T9900 nummod three,times
R4831 T9900 T9893 conj times,washed
R4832 T9901 T9900 prep in,times
R4833 T9902 T9901 pobj PBS,in
R4834 T9903 T9893 punct ", ",washed
R4835 T9904 T9905 nummod 5,min
R4836 T9905 T9893 npadvmod min,washed
R4837 T9906 T9905 appos each,min
R4838 T9907 T9893 punct ", ",washed
R4839 T9908 T9893 conj rinsed,washed
R4840 T9909 T9908 prep in,rinsed
R4841 T9910 T9909 pobj water,in
R4842 T9911 T9908 cc and,rinsed
R4843 T9912 T9908 conj embedded,rinsed
R4844 T9913 T9912 prep in,embedded
R4845 T9914 T9913 pobj gelvatol,in
R4846 T9915 T9916 punct [,36
R4847 T9916 T9912 parataxis 36,embedded
R4848 T9917 T9916 punct ],36
R4849 T9918 T9869 punct .,incubated
R4850 T9920 T9921 prep For,incubated
R4851 T9922 T9920 pobj control,For
R4852 T9923 T9921 punct ", ",incubated
R4853 T9924 T9921 nsubjpass sections,incubated
R4854 T9925 T9921 auxpass were,incubated
R4855 T9926 T9927 advmod only,with
R4856 T9927 T9921 prep with,incubated
R4857 T9928 T9929 det the,antibody
R4858 T9929 T9927 pobj antibody,with
R4859 T9930 T9929 amod secondary,antibody
R4860 T9931 T9921 cc or,incubated
R4861 T9932 T9933 nsubjpass sections,used
R4862 T9933 T9921 conj used,incubated
R4863 T9934 T9932 acl obtained,sections
R4864 T9935 T9934 prep from,obtained
R4865 T9936 T9937 det an,mouse
R4866 T9937 T9935 pobj mouse,from
R4867 T9938 T9939 compound annexin,A7
R4868 T9939 T9937 compound A7,mouse
R4869 T9940 T9937 compound knockout,mouse
R4870 T9941 T9933 auxpass were,used
R4871 T9942 T9943 punct [,10
R4872 T9943 T9933 parataxis 10,used
R4873 T9944 T9943 punct ],10
R4874 T9945 T9921 punct .,incubated
R4875 T9947 T9948 nsubjpass Samples,examined
R4876 T9949 T9948 auxpass were,examined
R4877 T9950 T9948 prep with,examined
R4878 T9951 T9952 det a,microscope
R4879 T9952 T9950 pobj microscope,with
R4880 T9953 T9952 amod confocal,microscope
R4881 T9954 T9952 compound laser,microscope
R4882 T9955 T9952 compound scan,microscope
R4883 T9956 T9957 punct (,SP
R4884 T9957 T9952 parataxis SP,microscope
R4885 T9958 T9957 compound Leica,SP
R4886 T9959 T9957 compound TCS,SP
R4887 T9960 T9957 punct ;,SP
R4888 T9961 T9957 appos Leica,SP
R4889 T9962 T9961 punct ", ",Leica
R4890 T9963 T9961 npadvmod Bensheim,Leica
R4891 T9964 T9961 punct ", ",Leica
R4892 T9965 T9961 npadvmod FRG,Leica
R4893 T9966 T9957 punct ),SP
R4894 T9967 T9948 punct .,examined