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PMC:1087847 / 31612-32595 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T8507 0-7 NNS denotes Animals
T8508 8-11 CC denotes and
T8509 12-18 NN denotes tissue
T8510 19-30 NN denotes preparation
T8511 30-59 sentence denotes 129SV strain mice were used.
T8512 31-36 NN denotes 129SV
T8513 37-43 NN denotes strain
T8514 44-48 NNS denotes mice
T8516 49-53 VBD denotes were
T8515 54-58 VBN denotes used
T8517 58-59 . denotes .
T8518 59-126 sentence denotes The day on which the vaginal plug was confirmed was defined as E0.
T8519 60-63 DT denotes The
T8520 64-67 NN denotes day
T8522 68-70 IN denotes on
T8524 71-76 WDT denotes which
T8525 77-80 DT denotes the
T8527 81-88 JJ denotes vaginal
T8526 89-93 NN denotes plug
T8528 94-97 VBD denotes was
T8523 98-107 VBN denotes confirmed
T8529 108-111 VBD denotes was
T8521 112-119 VBN denotes defined
T8530 120-122 IN denotes as
T8531 123-125 NN denotes E0
T8532 125-126 . denotes .
T8533 126-269 sentence denotes All the animal experiments were performed in accordance with the Guide for the Care and Use of Laboratory Animals (NIH Publication No. 85-23).
T8534 127-130 PDT denotes All
T8536 131-134 DT denotes the
T8537 135-141 NN denotes animal
T8535 142-153 NNS denotes experiments
T8539 154-158 VBD denotes were
T8538 159-168 VBN denotes performed
T8540 169-171 IN denotes in
T8541 172-182 NN denotes accordance
T8542 183-187 IN denotes with
T8543 188-191 DT denotes the
T8544 192-197 NNP denotes Guide
T8545 198-201 IN denotes for
T8546 202-205 DT denotes the
T8547 206-210 NNP denotes Care
T8548 211-214 CC denotes and
T8549 215-218 NNP denotes Use
T8550 219-221 IN denotes of
T8551 222-232 NNP denotes Laboratory
T8552 233-240 NNPS denotes Animals
T8553 241-242 -LRB- denotes (
T8554 242-245 NNP denotes NIH
T8555 246-257 NNP denotes Publication
T8556 258-261 NN denotes No.
T8557 262-264 CD denotes 85
T8558 264-265 HYPH denotes -
T8559 265-267 CD denotes 23
T8560 267-268 -RRB- denotes )
T8561 268-269 . denotes .
T8562 269-367 sentence denotes Pregnant females were sacrificed by cervical dislocation at various intervals between E5 and E17.
T8563 270-278 JJ denotes Pregnant
T8564 279-286 NNS denotes females
T8566 287-291 VBD denotes were
T8565 292-302 VBN denotes sacrificed
T8567 303-305 IN denotes by
T8568 306-314 JJ denotes cervical
T8569 315-326 NN denotes dislocation
T8570 327-329 IN denotes at
T8571 330-337 JJ denotes various
T8572 338-347 NNS denotes intervals
T8573 348-355 IN denotes between
T8574 356-358 NN denotes E5
T8575 359-362 CC denotes and
T8576 363-366 NN denotes E17
T8577 366-367 . denotes .
T8578 367-428 sentence denotes Before E9, embryos were embedded within the maternal uterus.
T8579 368-374 IN denotes Before
T8581 375-377 NN denotes E9
T8582 377-379 , denotes ,
T8583 379-386 NNS denotes embryos
T8584 387-391 VBD denotes were
T8580 392-400 VBN denotes embedded
T8585 401-407 IN denotes within
T8586 408-411 DT denotes the
T8588 412-420 JJ denotes maternal
T8587 421-427 NN denotes uterus
T8589 427-428 . denotes .
T8590 428-505 sentence denotes After E9, embryos were removed from their membranes and embedded separately.
T8591 429-434 IN denotes After
T8593 435-437 NN denotes E9
T8594 437-439 , denotes ,
T8595 439-446 NNS denotes embryos
T8596 447-451 VBD denotes were
T8592 452-459 VBN denotes removed
T8597 460-464 IN denotes from
T8598 465-470 PRP$ denotes their
T8599 471-480 NNS denotes membranes
T8600 481-484 CC denotes and
T8601 485-493 VBN denotes embedded
T8602 494-504 RB denotes separately
T8603 504-505 . denotes .
T8604 505-586 sentence denotes Experiments were performed with four to six animals at each developmental stage.
T8605 506-517 NNS denotes Experiments
T8607 518-522 VBD denotes were
T8606 523-532 VBN denotes performed
T8608 533-537 IN denotes with
T8609 538-542 CD denotes four
T8611 543-545 IN denotes to
T8610 546-549 CD denotes six
T8612 550-557 NNS denotes animals
T8613 558-560 IN denotes at
T8614 561-565 DT denotes each
T8616 566-579 JJ denotes developmental
T8615 580-585 NN denotes stage
T8617 585-586 . denotes .
T8618 586-661 sentence denotes The mature brain used was derived from mice of three to four month of age.
T8619 587-590 DT denotes The
T8621 591-597 JJ denotes mature
T8620 598-603 NN denotes brain
T8623 604-608 VBN denotes used
T8624 609-612 VBD denotes was
T8622 613-620 VBN denotes derived
T8625 621-625 IN denotes from
T8626 626-630 NNS denotes mice
T8627 631-633 IN denotes of
T8628 634-639 CD denotes three
T8630 640-642 IN denotes to
T8629 643-647 CD denotes four
T8631 648-653 NN denotes month
T8632 654-656 IN denotes of
T8633 657-660 NN denotes age
T8634 660-661 . denotes .
T8635 661-796 sentence denotes The embryos and tissues were fixed in freshly prepared 4% paraformaldehyde solution for 2–12 h depending on the tissue thickness [35].
T8636 662-665 DT denotes The
T8637 666-673 NNS denotes embryos
T8639 674-677 CC denotes and
T8640 678-685 NNS denotes tissues
T8641 686-690 VBD denotes were
T8638 691-696 VBN denotes fixed
T8642 697-699 IN denotes in
T8643 700-707 RB denotes freshly
T8644 708-716 VBN denotes prepared
T8646 717-718 CD denotes 4
T8647 718-719 NN denotes %
T8648 720-736 NN denotes paraformaldehyde
T8645 737-745 NN denotes solution
T8649 746-749 IN denotes for
T8650 750-751 CD denotes 2
T8652 751-752 SYM denotes –
T8651 752-754 CD denotes 12
T8653 755-756 NN denotes h
T8654 757-766 VBG denotes depending
T8655 767-769 IN denotes on
T8656 770-773 DT denotes the
T8658 774-780 NN denotes tissue
T8657 781-790 NN denotes thickness
T8659 791-792 -LRB- denotes [
T8660 792-794 CD denotes 35
T8661 794-795 -RRB- denotes ]
T8662 795-796 . denotes .
T8663 796-889 sentence denotes They were dehydrated with ethanol and embedded in paraffin according to standard procedures.
T8664 797-801 PRP denotes They
T8666 802-806 VBD denotes were
T8665 807-817 VBN denotes dehydrated
T8667 818-822 IN denotes with
T8668 823-830 NN denotes ethanol
T8669 831-834 CC denotes and
T8670 835-843 VBN denotes embedded
T8671 844-846 IN denotes in
T8672 847-855 NN denotes paraffin
T8673 856-865 VBG denotes according
T8674 866-868 IN denotes to
T8675 869-877 JJ denotes standard
T8676 878-888 NNS denotes procedures
T8677 888-889 . denotes .
T8678 889-983 sentence denotes Microtome sections were cut at 7–10 μm, placed on slides and stored until further processing.
T8679 890-899 NN denotes Microtome
T8680 900-908 NNS denotes sections
T8682 909-913 VBD denotes were
T8681 914-917 VBN denotes cut
T8683 918-920 IN denotes at
T8684 921-922 CD denotes 7
T8686 922-923 SYM denotes –
T8685 923-925 CD denotes 10
T8687 926-928 NN denotes μm
T8688 928-930 , denotes ,
T8689 930-936 VBN denotes placed
T8690 937-939 IN denotes on
T8691 940-946 NNS denotes slides
T8692 947-950 CC denotes and
T8693 951-957 VBN denotes stored
T8694 958-963 IN denotes until
T8695 964-971 JJ denotes further
T8696 972-982 NN denotes processing
T8697 982-983 . denotes .
R4166 T8508 T8507 cc and,Animals
R4167 T8509 T8510 compound tissue,preparation
R4168 T8510 T8507 conj preparation,Animals
R4169 T8512 T8513 compound 129SV,strain
R4170 T8513 T8514 compound strain,mice
R4171 T8514 T8515 nsubjpass mice,used
R4172 T8516 T8515 auxpass were,used
R4173 T8517 T8515 punct .,used
R4174 T8519 T8520 det The,day
R4175 T8520 T8521 nsubjpass day,defined
R4176 T8522 T8523 prep on,confirmed
R4177 T8523 T8520 relcl confirmed,day
R4178 T8524 T8522 pobj which,on
R4179 T8525 T8526 det the,plug
R4180 T8526 T8523 nsubjpass plug,confirmed
R4181 T8527 T8526 amod vaginal,plug
R4182 T8528 T8523 auxpass was,confirmed
R4183 T8529 T8521 auxpass was,defined
R4184 T8530 T8521 prep as,defined
R4185 T8531 T8530 pobj E0,as
R4186 T8532 T8521 punct .,defined
R4187 T8534 T8535 predet All,experiments
R4188 T8535 T8538 nsubjpass experiments,performed
R4189 T8536 T8535 det the,experiments
R4190 T8537 T8535 compound animal,experiments
R4191 T8539 T8538 auxpass were,performed
R4192 T8540 T8538 prep in,performed
R4193 T8541 T8540 pobj accordance,in
R4194 T8542 T8541 prep with,accordance
R4195 T8543 T8544 det the,Guide
R4196 T8544 T8542 pobj Guide,with
R4197 T8545 T8544 prep for,Guide
R4198 T8546 T8547 det the,Care
R4199 T8547 T8545 pobj Care,for
R4200 T8548 T8547 cc and,Care
R4201 T8549 T8547 conj Use,Care
R4202 T8550 T8547 prep of,Care
R4203 T8551 T8552 compound Laboratory,Animals
R4204 T8552 T8550 pobj Animals,of
R4205 T8553 T8554 punct (,NIH
R4206 T8554 T8544 meta NIH,Guide
R4207 T8555 T8554 nmod Publication,NIH
R4208 T8556 T8554 nmod No.,NIH
R4209 T8557 T8554 nummod 85,NIH
R4210 T8558 T8554 punct -,NIH
R4211 T8559 T8554 nummod 23,NIH
R4212 T8560 T8554 punct ),NIH
R4213 T8561 T8538 punct .,performed
R4214 T8563 T8564 amod Pregnant,females
R4215 T8564 T8565 nsubjpass females,sacrificed
R4216 T8566 T8565 auxpass were,sacrificed
R4217 T8567 T8565 prep by,sacrificed
R4218 T8568 T8569 amod cervical,dislocation
R4219 T8569 T8567 pobj dislocation,by
R4220 T8570 T8565 prep at,sacrificed
R4221 T8571 T8572 amod various,intervals
R4222 T8572 T8570 pobj intervals,at
R4223 T8573 T8572 prep between,intervals
R4224 T8574 T8573 pobj E5,between
R4225 T8575 T8574 cc and,E5
R4226 T8576 T8574 conj E17,E5
R4227 T8577 T8565 punct .,sacrificed
R4228 T8579 T8580 prep Before,embedded
R4229 T8581 T8579 pobj E9,Before
R4230 T8582 T8580 punct ", ",embedded
R4231 T8583 T8580 nsubjpass embryos,embedded
R4232 T8584 T8580 auxpass were,embedded
R4233 T8585 T8580 prep within,embedded
R4234 T8586 T8587 det the,uterus
R4235 T8587 T8585 pobj uterus,within
R4236 T8588 T8587 amod maternal,uterus
R4237 T8589 T8580 punct .,embedded
R4238 T8591 T8592 prep After,removed
R4239 T8593 T8591 pobj E9,After
R4240 T8594 T8592 punct ", ",removed
R4241 T8595 T8592 nsubjpass embryos,removed
R4242 T8596 T8592 auxpass were,removed
R4243 T8597 T8592 prep from,removed
R4244 T8598 T8599 poss their,membranes
R4245 T8599 T8597 pobj membranes,from
R4246 T8600 T8592 cc and,removed
R4247 T8601 T8592 conj embedded,removed
R4248 T8602 T8601 advmod separately,embedded
R4249 T8603 T8592 punct .,removed
R4250 T8605 T8606 nsubjpass Experiments,performed
R4251 T8607 T8606 auxpass were,performed
R4252 T8608 T8606 prep with,performed
R4253 T8609 T8610 quantmod four,six
R4254 T8610 T8612 nummod six,animals
R4255 T8611 T8610 quantmod to,six
R4256 T8612 T8608 pobj animals,with
R4257 T8613 T8606 prep at,performed
R4258 T8614 T8615 det each,stage
R4259 T8615 T8613 pobj stage,at
R4260 T8616 T8615 amod developmental,stage
R4261 T8617 T8606 punct .,performed
R4262 T8619 T8620 det The,brain
R4263 T8620 T8622 nsubjpass brain,derived
R4264 T8621 T8620 amod mature,brain
R4265 T8623 T8620 acl used,brain
R4266 T8624 T8622 auxpass was,derived
R4267 T8625 T8622 prep from,derived
R4268 T8626 T8625 pobj mice,from
R4269 T8627 T8626 prep of,mice
R4270 T8628 T8629 quantmod three,four
R4271 T8629 T8631 nummod four,month
R4272 T8630 T8629 quantmod to,four
R4273 T8631 T8627 pobj month,of
R4274 T8632 T8631 prep of,month
R4275 T8633 T8632 pobj age,of
R4276 T8634 T8622 punct .,derived
R4277 T8636 T8637 det The,embryos
R4278 T8637 T8638 nsubjpass embryos,fixed
R4279 T8639 T8637 cc and,embryos
R4280 T8640 T8637 conj tissues,embryos
R4281 T8641 T8638 auxpass were,fixed
R4282 T8642 T8638 prep in,fixed
R4283 T8643 T8644 advmod freshly,prepared
R4284 T8644 T8645 amod prepared,solution
R4285 T8645 T8642 pobj solution,in
R4286 T8646 T8647 nummod 4,%
R4287 T8647 T8645 compound %,solution
R4288 T8648 T8645 compound paraformaldehyde,solution
R4289 T8649 T8638 prep for,fixed
R4290 T8650 T8651 quantmod 2,12
R4291 T8651 T8653 nummod 12,h
R4292 T8652 T8651 punct –,12
R4293 T8653 T8649 pobj h,for
R4294 T8654 T8638 prep depending,fixed
R4295 T8655 T8654 prep on,depending
R4296 T8656 T8657 det the,thickness
R4297 T8657 T8655 pobj thickness,on
R4298 T8658 T8657 compound tissue,thickness
R4299 T8659 T8660 punct [,35
R4300 T8660 T8638 parataxis 35,fixed
R4301 T8661 T8660 punct ],35
R4302 T8662 T8638 punct .,fixed
R4303 T8664 T8665 nsubj They,dehydrated
R4304 T8666 T8665 aux were,dehydrated
R4305 T8667 T8665 prep with,dehydrated
R4306 T8668 T8667 pobj ethanol,with
R4307 T8669 T8665 cc and,dehydrated
R4308 T8670 T8665 conj embedded,dehydrated
R4309 T8671 T8670 prep in,embedded
R4310 T8672 T8671 pobj paraffin,in
R4311 T8673 T8665 prep according,dehydrated
R4312 T8674 T8673 prep to,according
R4313 T8675 T8676 amod standard,procedures
R4314 T8676 T8674 pobj procedures,to
R4315 T8677 T8665 punct .,dehydrated
R4316 T8679 T8680 compound Microtome,sections
R4317 T8680 T8681 nsubjpass sections,cut
R4318 T8682 T8681 auxpass were,cut
R4319 T8683 T8681 prep at,cut
R4320 T8684 T8685 quantmod 7,10
R4321 T8685 T8687 nummod 10,μm
R4322 T8686 T8685 punct –,10
R4323 T8687 T8683 pobj μm,at
R4324 T8688 T8681 punct ", ",cut
R4325 T8689 T8681 conj placed,cut
R4326 T8690 T8689 prep on,placed
R4327 T8691 T8690 pobj slides,on
R4328 T8692 T8689 cc and,placed
R4329 T8693 T8689 conj stored,placed
R4330 T8694 T8693 prep until,stored
R4331 T8695 T8696 amod further,processing
R4332 T8696 T8694 pobj processing,until
R4333 T8697 T8681 punct .,cut