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PMC:1087847 / 19244-22347 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T5136 0-8 NN denotes Presence
T5137 9-11 IN denotes of
T5138 12-19 NN denotes Annexin
T5139 20-22 NN denotes A7
T5140 23-25 IN denotes in
T5141 26-32 NNS denotes nuclei
T5142 33-37 IN denotes from
T5143 38-46 JJ denotes neuronal
T5145 47-50 CC denotes and
T5146 51-61 JJ denotes astroglial
T5144 62-67 NNS denotes cells
T5147 67-303 sentence denotes The nuclear localisation of Annexin A7 in mice observed in immature cells at E16 and differentiated adult neurons could be only detected as a strong signal when the brain sections were pre-treated with trypsin before antibody staining.
T5148 68-71 DT denotes The
T5150 72-79 JJ denotes nuclear
T5149 80-92 NN denotes localisation
T5152 93-95 IN denotes of
T5153 96-103 NN denotes Annexin
T5154 104-106 NN denotes A7
T5155 107-109 IN denotes in
T5156 110-114 NNS denotes mice
T5157 115-123 VBN denotes observed
T5158 124-126 IN denotes in
T5159 127-135 JJ denotes immature
T5160 136-141 NNS denotes cells
T5161 142-144 IN denotes at
T5162 145-148 NN denotes E16
T5163 149-152 CC denotes and
T5164 153-167 VBN denotes differentiated
T5166 168-173 JJ denotes adult
T5165 174-181 NNS denotes neurons
T5167 182-187 MD denotes could
T5168 188-190 VB denotes be
T5169 191-195 RB denotes only
T5151 196-204 VBN denotes detected
T5170 205-207 IN denotes as
T5171 208-209 DT denotes a
T5173 210-216 JJ denotes strong
T5172 217-223 NN denotes signal
T5174 224-228 WRB denotes when
T5176 229-232 DT denotes the
T5178 233-238 NN denotes brain
T5177 239-247 NNS denotes sections
T5179 248-252 VBD denotes were
T5175 253-264 VBN denotes pre-treated
T5180 265-269 IN denotes with
T5181 270-277 NN denotes trypsin
T5182 278-284 IN denotes before
T5183 285-293 NN denotes antibody
T5184 294-302 NN denotes staining
T5185 302-303 . denotes .
T5186 303-409 sentence denotes This procedure is thought to allow the antibodies to access epitopes masked by the formaldehyde fixation.
T5187 304-308 DT denotes This
T5188 309-318 NN denotes procedure
T5190 319-321 VBZ denotes is
T5189 322-329 VBN denotes thought
T5191 330-332 TO denotes to
T5192 333-338 VB denotes allow
T5193 339-342 DT denotes the
T5194 343-353 NNS denotes antibodies
T5196 354-356 TO denotes to
T5195 357-363 VB denotes access
T5197 364-372 NNS denotes epitopes
T5198 373-379 VBN denotes masked
T5199 380-382 IN denotes by
T5200 383-386 DT denotes the
T5202 387-399 NN denotes formaldehyde
T5201 400-408 NN denotes fixation
T5203 408-409 . denotes .
T5204 409-474 sentence denotes Methanol fixation of cultured cells led to inconsistent results.
T5205 410-418 NN denotes Methanol
T5206 419-427 NN denotes fixation
T5208 428-430 IN denotes of
T5209 431-439 VBN denotes cultured
T5210 440-445 NNS denotes cells
T5207 446-449 VBD denotes led
T5211 450-452 IN denotes to
T5212 453-465 JJ denotes inconsistent
T5213 466-473 NNS denotes results
T5214 473-474 . denotes .
T5215 474-685 sentence denotes Antigen retrieval in formalin-fixed and paraffin-embedded tissue sections employs various heating or proteolytic pre-treatment methods [[15-17]; Ein Handbuch für die Histologie, dianova GmbH, Hamburg, Germany].
T5216 475-482 NN denotes Antigen
T5217 483-492 NN denotes retrieval
T5219 493-495 IN denotes in
T5220 496-504 NN denotes formalin
T5222 504-505 HYPH denotes -
T5221 505-510 VBN denotes fixed
T5224 511-514 CC denotes and
T5225 515-523 NN denotes paraffin
T5227 523-524 HYPH denotes -
T5226 524-532 VBN denotes embedded
T5228 533-539 NN denotes tissue
T5223 540-548 NNS denotes sections
T5218 549-556 VBZ denotes employs
T5229 557-564 JJ denotes various
T5231 565-572 NN denotes heating
T5232 573-575 CC denotes or
T5233 576-587 JJ denotes proteolytic
T5234 588-601 NN denotes pre-treatment
T5230 602-609 NNS denotes methods
T5235 610-611 -LRB- denotes [
T5236 611-612 -LRB- denotes [
T5237 612-614 CD denotes 15
T5238 614-615 SYM denotes -
T5239 615-617 CD denotes 17
T5240 617-618 -RRB- denotes ]
T5241 618-619 : denotes ;
T5242 620-623 NNP denotes Ein
T5243 624-632 NNP denotes Handbuch
T5244 633-636 NNP denotes für
T5245 637-640 NNP denotes die
T5246 641-651 NNP denotes Histologie
T5247 651-653 , denotes ,
T5248 653-660 NNP denotes dianova
T5249 661-665 NNP denotes GmbH
T5250 665-667 , denotes ,
T5251 667-674 NNP denotes Hamburg
T5252 674-676 , denotes ,
T5253 676-683 NNP denotes Germany
T5254 683-684 -RRB- denotes ]
T5255 684-685 . denotes .
T5256 685-820 sentence denotes These methods can result in moderate or strong specific antibody staining, but the detectability of other antigens might be decreased.
T5257 686-691 DT denotes These
T5258 692-699 NNS denotes methods
T5260 700-703 MD denotes can
T5259 704-710 VB denotes result
T5261 711-713 IN denotes in
T5262 714-722 JJ denotes moderate
T5264 723-725 CC denotes or
T5265 726-732 JJ denotes strong
T5266 733-741 JJ denotes specific
T5267 742-750 NN denotes antibody
T5263 751-759 NN denotes staining
T5268 759-761 , denotes ,
T5269 761-764 CC denotes but
T5270 765-768 DT denotes the
T5271 769-782 NN denotes detectability
T5273 783-785 IN denotes of
T5274 786-791 JJ denotes other
T5275 792-800 NNS denotes antigens
T5276 801-806 MD denotes might
T5277 807-809 VB denotes be
T5272 810-819 VBN denotes decreased
T5278 819-820 . denotes .
T5279 820-899 sentence denotes Moreover, the optimal pre-treatment has to be individualised for each antigen.
T5280 821-829 RB denotes Moreover
T5282 829-831 , denotes ,
T5283 831-834 DT denotes the
T5285 835-842 JJ denotes optimal
T5284 843-856 NN denotes pre-treatment
T5281 857-860 VBZ denotes has
T5286 861-863 TO denotes to
T5288 864-866 VB denotes be
T5287 867-881 VBN denotes individualised
T5289 882-885 IN denotes for
T5290 886-890 DT denotes each
T5291 891-898 NN denotes antigen
T5292 898-899 . denotes .
T5293 899-1133 sentence denotes To verify the presence of endogenous Annexin A7 in nuclei, we used a biochemical approach and purified nuclei from neuro-2a, PC-12, and C6 cells and treated them with a hypotonic extraction buffer to obtain the nucleoplasm (Fig. 7A).
T5294 900-902 TO denotes To
T5295 903-909 VB denotes verify
T5297 910-913 DT denotes the
T5298 914-922 NN denotes presence
T5299 923-925 IN denotes of
T5300 926-936 JJ denotes endogenous
T5302 937-944 NN denotes Annexin
T5301 945-947 NN denotes A7
T5303 948-950 IN denotes in
T5304 951-957 NNS denotes nuclei
T5305 957-959 , denotes ,
T5306 959-961 PRP denotes we
T5296 962-966 VBD denotes used
T5307 967-968 DT denotes a
T5309 969-980 JJ denotes biochemical
T5308 981-989 NN denotes approach
T5310 990-993 CC denotes and
T5311 994-1002 VBN denotes purified
T5312 1003-1009 NNS denotes nuclei
T5313 1010-1014 IN denotes from
T5314 1015-1020 NN denotes neuro
T5316 1020-1021 HYPH denotes -
T5317 1021-1023 CD denotes 2a
T5318 1023-1025 , denotes ,
T5319 1025-1027 NN denotes PC
T5320 1027-1028 HYPH denotes -
T5321 1028-1030 CD denotes 12
T5322 1030-1032 , denotes ,
T5323 1032-1035 CC denotes and
T5324 1036-1038 NN denotes C6
T5315 1039-1044 NNS denotes cells
T5325 1045-1048 CC denotes and
T5326 1049-1056 VBN denotes treated
T5327 1057-1061 PRP denotes them
T5328 1062-1066 IN denotes with
T5329 1067-1068 DT denotes a
T5331 1069-1078 JJ denotes hypotonic
T5332 1079-1089 NN denotes extraction
T5330 1090-1096 NN denotes buffer
T5333 1097-1099 TO denotes to
T5334 1100-1106 VB denotes obtain
T5335 1107-1110 DT denotes the
T5336 1111-1122 NN denotes nucleoplasm
T5337 1123-1124 -LRB- denotes (
T5339 1124-1128 NN denotes Fig.
T5338 1129-1131 NN denotes 7A
T5340 1131-1132 -RRB- denotes )
T5341 1132-1133 . denotes .
T5342 1133-1281 sentence denotes The nucleoplasm and the remaining nuclei, from which the nucleoplasm has been extracted partially, were subjected to SDS-PAGE and Western blotting.
T5343 1134-1137 DT denotes The
T5344 1138-1149 NN denotes nucleoplasm
T5346 1150-1153 CC denotes and
T5347 1154-1157 DT denotes the
T5349 1158-1167 VBG denotes remaining
T5348 1168-1174 NNS denotes nuclei
T5350 1174-1176 , denotes ,
T5351 1176-1180 IN denotes from
T5353 1181-1186 WDT denotes which
T5354 1187-1190 DT denotes the
T5355 1191-1202 NN denotes nucleoplasm
T5356 1203-1206 VBZ denotes has
T5357 1207-1211 VBN denotes been
T5352 1212-1221 VBN denotes extracted
T5358 1222-1231 RB denotes partially
T5359 1231-1233 , denotes ,
T5360 1233-1237 VBD denotes were
T5345 1238-1247 VBN denotes subjected
T5361 1248-1250 IN denotes to
T5362 1251-1254 NN denotes SDS
T5364 1254-1255 HYPH denotes -
T5363 1255-1259 NN denotes PAGE
T5365 1260-1263 CC denotes and
T5366 1264-1271 NNP denotes Western
T5367 1272-1280 NN denotes blotting
T5368 1280-1281 . denotes .
T5369 1281-1519 sentence denotes Western blots probed with mAb 203–217 showed Annexin A7 in the nucleoplasm of neuronal (neuro-2a, PC-12) and astroglial (C6) cell lines (Fig. 7A), however, the large isoform could only be clearly extracted with nucleoplasm from C6 cells.
T5370 1282-1289 NNP denotes Western
T5371 1290-1295 NNS denotes blots
T5373 1296-1302 VBN denotes probed
T5374 1303-1307 IN denotes with
T5375 1308-1311 NN denotes mAb
T5376 1312-1315 CD denotes 203
T5377 1315-1316 SYM denotes –
T5378 1316-1319 CD denotes 217
T5372 1320-1326 VBD denotes showed
T5380 1327-1334 NN denotes Annexin
T5381 1335-1337 NN denotes A7
T5382 1338-1340 IN denotes in
T5383 1341-1344 DT denotes the
T5384 1345-1356 NN denotes nucleoplasm
T5385 1357-1359 IN denotes of
T5386 1360-1368 JJ denotes neuronal
T5388 1369-1370 -LRB- denotes (
T5390 1370-1375 NN denotes neuro
T5391 1375-1376 HYPH denotes -
T5389 1376-1378 NN denotes 2a
T5392 1378-1380 , denotes ,
T5393 1380-1382 NN denotes PC
T5394 1382-1383 HYPH denotes -
T5395 1383-1385 CD denotes 12
T5396 1385-1386 -RRB- denotes )
T5397 1387-1390 CC denotes and
T5398 1391-1401 JJ denotes astroglial
T5399 1402-1403 -LRB- denotes (
T5400 1403-1405 NN denotes C6
T5401 1405-1406 -RRB- denotes )
T5402 1407-1411 NN denotes cell
T5387 1412-1417 NNS denotes lines
T5403 1418-1419 -LRB- denotes (
T5405 1419-1423 NN denotes Fig.
T5404 1424-1426 NN denotes 7A
T5406 1426-1427 -RRB- denotes )
T5407 1427-1429 , denotes ,
T5408 1429-1436 RB denotes however
T5409 1436-1438 , denotes ,
T5410 1438-1441 DT denotes the
T5412 1442-1447 JJ denotes large
T5411 1448-1455 NN denotes isoform
T5413 1456-1461 MD denotes could
T5414 1462-1466 RB denotes only
T5415 1467-1469 VB denotes be
T5416 1470-1477 RB denotes clearly
T5379 1478-1487 VBN denotes extracted
T5417 1488-1492 IN denotes with
T5418 1493-1504 NN denotes nucleoplasm
T5419 1505-1509 IN denotes from
T5420 1510-1512 NN denotes C6
T5421 1513-1518 NNS denotes cells
T5422 1518-1519 . denotes .
T5423 1519-1803 sentence denotes For control, antibodies against LAP2α, Emerin and tubulin were used to verify that the nucleoplasm (marker:LAP2α, which is anon-membrane-bound isoform of LAP2) was successfully extracted and also not contaminated by nuclear membranes (marker:Emerin) or by cytoplasm (marker:tubulin).
T5424 1520-1523 IN denotes For
T5426 1524-1531 NN denotes control
T5427 1531-1533 , denotes ,
T5428 1533-1543 NNS denotes antibodies
T5429 1544-1551 IN denotes against
T5430 1552-1557 NN denotes LAP2α
T5431 1557-1559 , denotes ,
T5432 1559-1565 NN denotes Emerin
T5433 1566-1569 CC denotes and
T5434 1570-1577 NN denotes tubulin
T5435 1578-1582 VBD denotes were
T5425 1583-1587 VBN denotes used
T5436 1588-1590 TO denotes to
T5437 1591-1597 VB denotes verify
T5438 1598-1602 IN denotes that
T5440 1603-1606 DT denotes the
T5441 1607-1618 NN denotes nucleoplasm
T5442 1619-1620 -LRB- denotes (
T5444 1620-1626 NN denotes marker
T5445 1626-1627 : denotes :
T5443 1627-1632 NN denotes LAP2α
T5446 1632-1634 , denotes ,
T5447 1634-1639 WDT denotes which
T5448 1640-1642 VBZ denotes is
T5449 1643-1647 NN denotes anon
T5451 1647-1648 HYPH denotes -
T5450 1648-1656 NN denotes membrane
T5453 1656-1657 HYPH denotes -
T5452 1657-1662 VBN denotes bound
T5454 1663-1670 NN denotes isoform
T5455 1671-1673 IN denotes of
T5456 1674-1678 NN denotes LAP2
T5457 1678-1679 -RRB- denotes )
T5458 1680-1683 VBD denotes was
T5459 1684-1696 RB denotes successfully
T5439 1697-1706 VBN denotes extracted
T5460 1707-1710 CC denotes and
T5461 1711-1715 RB denotes also
T5462 1716-1719 RB denotes not
T5463 1720-1732 VBN denotes contaminated
T5464 1733-1735 IN denotes by
T5465 1736-1743 JJ denotes nuclear
T5466 1744-1753 NNS denotes membranes
T5467 1754-1755 -LRB- denotes (
T5469 1755-1761 NN denotes marker
T5470 1761-1762 : denotes :
T5468 1762-1768 NN denotes Emerin
T5471 1768-1769 -RRB- denotes )
T5472 1770-1772 CC denotes or
T5473 1773-1775 IN denotes by
T5474 1776-1785 NN denotes cytoplasm
T5475 1786-1787 -LRB- denotes (
T5477 1787-1793 NN denotes marker
T5478 1793-1794 : denotes :
T5476 1794-1801 NN denotes tubulin
T5479 1801-1802 -RRB- denotes )
T5480 1802-1803 . denotes .
T5481 1803-1953 sentence denotes Likewise, in these neuronal and astroglial cell lines Annexin A7 had been observed in the nucleus by immunofluorescence (Fig. 7B and data not shown).
T5482 1804-1812 RB denotes Likewise
T5484 1812-1814 , denotes ,
T5485 1814-1816 IN denotes in
T5486 1817-1822 DT denotes these
T5488 1823-1831 JJ denotes neuronal
T5489 1832-1835 CC denotes and
T5490 1836-1846 JJ denotes astroglial
T5491 1847-1851 NN denotes cell
T5487 1852-1857 NNS denotes lines
T5492 1858-1865 NN denotes Annexin
T5493 1866-1868 NN denotes A7
T5494 1869-1872 VBD denotes had
T5495 1873-1877 VBN denotes been
T5483 1878-1886 VBN denotes observed
T5496 1887-1889 IN denotes in
T5497 1890-1893 DT denotes the
T5498 1894-1901 NN denotes nucleus
T5499 1902-1904 IN denotes by
T5500 1905-1923 NN denotes immunofluorescence
T5501 1924-1925 -LRB- denotes (
T5503 1925-1929 NN denotes Fig.
T5502 1930-1932 NN denotes 7B
T5504 1933-1936 CC denotes and
T5505 1937-1941 NNS denotes data
T5507 1942-1945 RB denotes not
T5506 1946-1951 VBN denotes shown
T5508 1951-1952 -RRB- denotes )
T5509 1952-1953 . denotes .
T5510 1953-2130 sentence denotes We noticed no difference between PC-12 and neuro-2a cells, however, as for the brain sections, we found an increase of the Annexin A7 staining after pre-treatment with trypsin.
T5511 1954-1956 PRP denotes We
T5512 1957-1964 VBD denotes noticed
T5514 1965-1967 DT denotes no
T5515 1968-1978 NN denotes difference
T5516 1979-1986 IN denotes between
T5517 1987-1989 NN denotes PC
T5519 1989-1990 HYPH denotes -
T5520 1990-1992 CD denotes 12
T5521 1993-1996 CC denotes and
T5522 1997-2002 NN denotes neuro
T5524 2002-2003 HYPH denotes -
T5523 2003-2005 NN denotes 2a
T5518 2006-2011 NNS denotes cells
T5525 2011-2013 , denotes ,
T5526 2013-2020 RB denotes however
T5527 2020-2022 , denotes ,
T5528 2022-2024 IN denotes as
T5529 2025-2028 IN denotes for
T5530 2029-2032 DT denotes the
T5532 2033-2038 NN denotes brain
T5531 2039-2047 NNS denotes sections
T5533 2047-2049 , denotes ,
T5534 2049-2051 PRP denotes we
T5513 2052-2057 VBD denotes found
T5535 2058-2060 DT denotes an
T5536 2061-2069 NN denotes increase
T5537 2070-2072 IN denotes of
T5538 2073-2076 DT denotes the
T5540 2077-2084 NN denotes Annexin
T5541 2085-2087 NN denotes A7
T5539 2088-2096 NN denotes staining
T5542 2097-2102 IN denotes after
T5543 2103-2116 NN denotes pre-treatment
T5544 2117-2121 IN denotes with
T5545 2122-2129 NN denotes trypsin
T5546 2129-2130 . denotes .
T14995 2141-2148 JJ denotes Nuclear
T14996 2149-2161 NN denotes localization
T14997 2162-2164 IN denotes of
T14998 2165-2172 NN denotes Annexin
T14999 2173-2175 NN denotes A7
T15000 2175-2176 . denotes .
T15001 2176-2301 sentence denotes (A) Extraction of Annexin A7 from nuclei of neuronal (PC-12, N2A) and an astroglial (C6) cell line using a hypotonic buffer.
T15002 2177-2178 -LRB- denotes (
T15003 2178-2179 LS denotes A
T15005 2179-2180 -RRB- denotes )
T15004 2181-2191 NN denotes Extraction
T15006 2192-2194 IN denotes of
T15007 2195-2202 NN denotes Annexin
T15008 2203-2205 NN denotes A7
T15009 2206-2210 IN denotes from
T15010 2211-2217 NNS denotes nuclei
T15011 2218-2220 IN denotes of
T15012 2221-2229 JJ denotes neuronal
T15013 2230-2231 -LRB- denotes (
T15014 2231-2233 NN denotes PC
T15015 2233-2234 HYPH denotes -
T15016 2234-2236 CD denotes 12
T15017 2236-2238 , denotes ,
T15018 2238-2241 NN denotes N2A
T15019 2241-2242 -RRB- denotes )
T15020 2243-2246 CC denotes and
T15021 2247-2249 DT denotes an
T15022 2250-2260 JJ denotes astroglial
T15024 2261-2262 -LRB- denotes (
T15025 2262-2264 NN denotes C6
T15026 2264-2265 -RRB- denotes )
T15027 2266-2270 NN denotes cell
T15023 2271-2275 NN denotes line
T15028 2276-2281 VBG denotes using
T15029 2282-2283 DT denotes a
T15031 2284-2293 JJ denotes hypotonic
T15030 2294-2300 NN denotes buffer
T15032 2300-2301 . denotes .
T15033 2301-2449 sentence denotes Samples of total cells (c), and corresponding amounts of nuclear membranes (m) and nucleoplasm (p) were subjected to SDS-PAGE and Western blotting.
T15034 2302-2309 NNS denotes Samples
T15036 2310-2312 IN denotes of
T15037 2313-2318 JJ denotes total
T15038 2319-2324 NNS denotes cells
T15039 2325-2326 -LRB- denotes (
T15040 2326-2327 NN denotes c
T15041 2327-2328 -RRB- denotes )
T15042 2328-2330 , denotes ,
T15043 2330-2333 CC denotes and
T15044 2334-2347 JJ denotes corresponding
T15045 2348-2355 NNS denotes amounts
T15046 2356-2358 IN denotes of
T15047 2359-2366 JJ denotes nuclear
T15048 2367-2376 NNS denotes membranes
T15049 2377-2378 -LRB- denotes (
T15050 2378-2379 NN denotes m
T15051 2379-2380 -RRB- denotes )
T15052 2381-2384 CC denotes and
T15053 2385-2396 NN denotes nucleoplasm
T15054 2397-2398 -LRB- denotes (
T15055 2398-2399 NN denotes p
T15056 2399-2400 -RRB- denotes )
T15057 2401-2405 VBD denotes were
T15035 2406-2415 VBN denotes subjected
T15058 2416-2418 IN denotes to
T15059 2419-2422 NN denotes SDS
T15061 2422-2423 HYPH denotes -
T15060 2423-2427 NN denotes PAGE
T15062 2428-2431 CC denotes and
T15063 2432-2439 NNP denotes Western
T15064 2440-2448 NN denotes blotting
T15065 2448-2449 . denotes .
T15066 2449-2573 sentence denotes Annexin A7 (AnxA7) isoforms are extractable from the nucleus of the indicated cells (47 kDa and 51 kDa isoform, arrowhead).
T15067 2450-2457 NN denotes Annexin
T15068 2458-2460 NN denotes A7
T15070 2461-2462 -LRB- denotes (
T15071 2462-2467 NN denotes AnxA7
T15072 2467-2468 -RRB- denotes )
T15069 2469-2477 NNS denotes isoforms
T15073 2478-2481 VBP denotes are
T15074 2482-2493 JJ denotes extractable
T15075 2494-2498 IN denotes from
T15076 2499-2502 DT denotes the
T15077 2503-2510 NN denotes nucleus
T15078 2511-2513 IN denotes of
T15079 2514-2517 DT denotes the
T15081 2518-2527 VBN denotes indicated
T15080 2528-2533 NNS denotes cells
T15082 2534-2535 -LRB- denotes (
T15084 2535-2537 CD denotes 47
T15085 2538-2541 NN denotes kDa
T15087 2542-2545 CC denotes and
T15088 2546-2548 CD denotes 51
T15089 2549-2552 NN denotes kDa
T15086 2553-2560 NN denotes isoform
T15090 2560-2562 , denotes ,
T15083 2562-2571 NN denotes arrowhead
T15091 2571-2572 -RRB- denotes )
T15092 2572-2573 . denotes .
T15093 2573-2805 sentence denotes For control, immunoblotting of LAP2α (75 kDa; non-membrane-bound isoform), Emerin (EM, 34 kDa), and tubulin (TB, 55 kDa) which are specific for nucleoplasm (LAP2α), nuclear membranes (Emerin), and the cytoplasm (tubulin) are shown.
T15094 2574-2577 IN denotes For
T15096 2578-2585 NN denotes control
T15097 2585-2587 , denotes ,
T15098 2587-2601 NN denotes immunoblotting
T15099 2602-2604 IN denotes of
T15100 2605-2610 NN denotes LAP2α
T15101 2611-2612 -LRB- denotes (
T15103 2612-2614 CD denotes 75
T15104 2615-2618 NN denotes kDa
T15105 2618-2619 : denotes ;
T15106 2620-2632 JJ denotes non-membrane
T15108 2632-2633 HYPH denotes -
T15107 2633-2638 VBN denotes bound
T15102 2639-2646 NN denotes isoform
T15109 2646-2647 -RRB- denotes )
T15110 2647-2649 , denotes ,
T15111 2649-2655 NN denotes Emerin
T15112 2656-2657 -LRB- denotes (
T15113 2657-2659 NN denotes EM
T15114 2659-2661 , denotes ,
T15116 2661-2663 CD denotes 34
T15115 2664-2667 NN denotes kDa
T15117 2667-2668 -RRB- denotes )
T15118 2668-2670 , denotes ,
T15119 2670-2673 CC denotes and
T15120 2674-2681 NN denotes tubulin
T15121 2682-2683 -LRB- denotes (
T15122 2683-2685 NN denotes TB
T15123 2685-2687 , denotes ,
T15125 2687-2689 CD denotes 55
T15124 2690-2693 NN denotes kDa
T15126 2693-2694 -RRB- denotes )
T15127 2695-2700 WDT denotes which
T15128 2701-2704 VBP denotes are
T15129 2705-2713 JJ denotes specific
T15130 2714-2717 IN denotes for
T15131 2718-2729 NN denotes nucleoplasm
T15132 2730-2731 -LRB- denotes (
T15133 2731-2736 NN denotes LAP2α
T15134 2736-2737 -RRB- denotes )
T15135 2737-2739 , denotes ,
T15136 2739-2746 JJ denotes nuclear
T15137 2747-2756 NNS denotes membranes
T15138 2757-2758 -LRB- denotes (
T15139 2758-2764 NN denotes Emerin
T15140 2764-2765 -RRB- denotes )
T15141 2765-2767 , denotes ,
T15142 2767-2770 CC denotes and
T15143 2771-2774 DT denotes the
T15144 2775-2784 NN denotes cytoplasm
T15145 2785-2786 -LRB- denotes (
T15146 2786-2793 NN denotes tubulin
T15147 2793-2794 -RRB- denotes )
T15148 2795-2798 VBP denotes are
T15095 2799-2804 VBN denotes shown
T15149 2804-2805 . denotes .
T15150 2805-2862 sentence denotes Note that the nucleoplasm is only partially extractable.
T15151 2806-2810 VB denotes Note
T15152 2811-2815 IN denotes that
T15154 2816-2819 DT denotes the
T15155 2820-2831 NN denotes nucleoplasm
T15153 2832-2834 VBZ denotes is
T15156 2835-2839 RB denotes only
T15157 2840-2849 RB denotes partially
T15158 2850-2861 JJ denotes extractable
T15159 2861-2862 . denotes .
T15160 2862-2917 sentence denotes (B) Immunofluorescence images of the PC-12 cells used.
T15161 2863-2864 -LRB- denotes (
T15162 2864-2865 LS denotes B
T15164 2865-2866 -RRB- denotes )
T15165 2867-2885 NN denotes Immunofluorescence
T15163 2886-2892 NNS denotes images
T15166 2893-2895 IN denotes of
T15167 2896-2899 DT denotes the
T15169 2900-2902 NN denotes PC
T15170 2902-2903 HYPH denotes -
T15171 2903-2905 CD denotes 12
T15168 2906-2911 NNS denotes cells
T15172 2912-2916 VBN denotes used
T15173 2916-2917 . denotes .
T15174 2917-3103 sentence denotes Cells were fixed with paraformaldehyde, permeabilized with Triton X-100, pretreated with trypsin as indicated, and stained with Annexin A7 specific mAb 203–217, DAPI in blue, bar 10 μm.
T15175 2918-2923 NNS denotes Cells
T15177 2924-2928 VBD denotes were
T15176 2929-2934 VBN denotes fixed
T15178 2935-2939 IN denotes with
T15179 2940-2956 NN denotes paraformaldehyde
T15180 2956-2958 , denotes ,
T15181 2958-2971 VBN denotes permeabilized
T15182 2972-2976 IN denotes with
T15183 2977-2983 NN denotes Triton
T15184 2984-2985 NN denotes X
T15185 2985-2986 HYPH denotes -
T15186 2986-2989 CD denotes 100
T15187 2989-2991 , denotes ,
T15188 2991-3001 VBN denotes pretreated
T15189 3002-3006 IN denotes with
T15190 3007-3014 NN denotes trypsin
T15191 3015-3017 IN denotes as
T15192 3018-3027 VBN denotes indicated
T15193 3027-3029 , denotes ,
T15194 3029-3032 CC denotes and
T15195 3033-3040 VBN denotes stained
T15196 3041-3045 IN denotes with
T15197 3046-3053 NN denotes Annexin
T15198 3054-3056 NN denotes A7
T15199 3057-3065 JJ denotes specific
T15201 3066-3069 NN denotes mAb
T15200 3070-3073 CD denotes 203
T15202 3073-3074 SYM denotes –
T15203 3074-3077 CD denotes 217
T15204 3077-3079 , denotes ,
T15206 3079-3083 NN denotes DAPI
T15207 3084-3086 IN denotes in
T15208 3087-3091 JJ denotes blue
T15209 3091-3093 , denotes ,
T15210 3093-3096 NN denotes bar
T15211 3097-3099 CD denotes 10
T15205 3100-3102 NN denotes μm
T15212 3102-3103 . denotes .
R2180 T5137 T5136 prep of,Presence
R2181 T5138 T5139 compound Annexin,A7
R2182 T5139 T5137 pobj A7,of
R2183 T5140 T5136 prep in,Presence
R2184 T5141 T5140 pobj nuclei,in
R2185 T5142 T5141 prep from,nuclei
R2186 T5143 T5144 amod neuronal,cells
R2187 T5144 T5142 pobj cells,from
R2188 T5145 T5143 cc and,neuronal
R2189 T5146 T5143 conj astroglial,neuronal
R2190 T5148 T5149 det The,localisation
R2191 T5149 T5151 nsubjpass localisation,detected
R2192 T5150 T5149 amod nuclear,localisation
R2193 T5152 T5149 prep of,localisation
R2194 T5153 T5154 compound Annexin,A7
R2195 T5154 T5152 pobj A7,of
R2196 T5155 T5149 prep in,localisation
R2197 T5156 T5155 pobj mice,in
R2198 T5157 T5149 acl observed,localisation
R2199 T5158 T5157 prep in,observed
R2200 T5159 T5160 amod immature,cells
R2201 T5160 T5158 pobj cells,in
R2202 T5161 T5160 prep at,cells
R2203 T5162 T5161 pobj E16,at
R2204 T5163 T5160 cc and,cells
R2205 T5164 T5165 amod differentiated,neurons
R2206 T5165 T5160 conj neurons,cells
R2207 T5166 T5165 amod adult,neurons
R2208 T5167 T5151 aux could,detected
R2209 T5168 T5151 auxpass be,detected
R2210 T5169 T5151 advmod only,detected
R2211 T5170 T5151 prep as,detected
R2212 T5171 T5172 det a,signal
R2213 T5172 T5170 pobj signal,as
R2214 T5173 T5172 amod strong,signal
R2215 T5174 T5175 advmod when,pre-treated
R2216 T5175 T5151 advcl pre-treated,detected
R2217 T5176 T5177 det the,sections
R2218 T5177 T5175 nsubjpass sections,pre-treated
R2219 T5178 T5177 compound brain,sections
R2220 T5179 T5175 auxpass were,pre-treated
R2221 T5180 T5175 prep with,pre-treated
R2222 T5181 T5180 pobj trypsin,with
R2223 T5182 T5175 prep before,pre-treated
R2224 T5183 T5184 compound antibody,staining
R2225 T5184 T5182 pobj staining,before
R2226 T5185 T5151 punct .,detected
R2227 T5187 T5188 det This,procedure
R2228 T5188 T5189 nsubjpass procedure,thought
R2229 T5190 T5189 auxpass is,thought
R2230 T5191 T5192 aux to,allow
R2231 T5192 T5189 xcomp allow,thought
R2232 T5193 T5194 det the,antibodies
R2233 T5194 T5195 nsubj antibodies,access
R2234 T5195 T5192 ccomp access,allow
R2235 T5196 T5195 aux to,access
R2236 T5197 T5195 dobj epitopes,access
R2237 T5198 T5197 acl masked,epitopes
R2238 T5199 T5198 agent by,masked
R2239 T5200 T5201 det the,fixation
R2240 T5201 T5199 pobj fixation,by
R2241 T5202 T5201 compound formaldehyde,fixation
R2242 T5203 T5189 punct .,thought
R2243 T5205 T5206 compound Methanol,fixation
R2244 T5206 T5207 nsubj fixation,led
R2245 T5208 T5206 prep of,fixation
R2246 T5209 T5210 amod cultured,cells
R2247 T5210 T5208 pobj cells,of
R2248 T5211 T5207 prep to,led
R2249 T5212 T5213 amod inconsistent,results
R2250 T5213 T5211 pobj results,to
R2251 T5214 T5207 punct .,led
R2252 T5216 T5217 compound Antigen,retrieval
R2253 T5217 T5218 nsubj retrieval,employs
R2254 T5219 T5217 prep in,retrieval
R2255 T5220 T5221 npadvmod formalin,fixed
R2256 T5221 T5223 amod fixed,sections
R2257 T5222 T5221 punct -,fixed
R2258 T5223 T5219 pobj sections,in
R2259 T5224 T5221 cc and,fixed
R2260 T5225 T5226 npadvmod paraffin,embedded
R2261 T5226 T5221 conj embedded,fixed
R2262 T5227 T5226 punct -,embedded
R2263 T5228 T5223 compound tissue,sections
R2264 T5229 T5230 amod various,methods
R2265 T5230 T5218 dobj methods,employs
R2266 T5231 T5230 nmod heating,methods
R2267 T5232 T5231 cc or,heating
R2268 T5233 T5234 amod proteolytic,pre-treatment
R2269 T5234 T5231 conj pre-treatment,heating
R2270 T5235 T5218 punct [,employs
R2271 T5236 T5237 punct [,15
R2272 T5237 T5218 parataxis 15,employs
R2273 T5238 T5239 punct -,17
R2274 T5239 T5237 prep 17,15
R2275 T5240 T5237 punct ],15
R2276 T5241 T5242 punct ;,Ein
R2277 T5242 T5218 meta Ein,employs
R2278 T5243 T5242 nmod Handbuch,Ein
R2279 T5244 T5242 nmod für,Ein
R2280 T5245 T5242 nmod die,Ein
R2281 T5246 T5242 nmod Histologie,Ein
R2282 T5247 T5242 punct ", ",Ein
R2283 T5248 T5242 nmod dianova,Ein
R2284 T5249 T5242 nmod GmbH,Ein
R2285 T5250 T5242 punct ", ",Ein
R2286 T5251 T5242 nmod Hamburg,Ein
R2287 T5252 T5242 punct ", ",Ein
R2288 T5253 T5242 nmod Germany,Ein
R2289 T5254 T5242 punct ],Ein
R2290 T5255 T5218 punct .,employs
R2291 T5257 T5258 det These,methods
R2292 T5258 T5259 nsubj methods,result
R2293 T5260 T5259 aux can,result
R2294 T5261 T5259 prep in,result
R2295 T5262 T5263 amod moderate,staining
R2296 T5263 T5261 pobj staining,in
R2297 T5264 T5262 cc or,moderate
R2298 T5265 T5262 conj strong,moderate
R2299 T5266 T5263 amod specific,staining
R2300 T5267 T5263 compound antibody,staining
R2301 T5268 T5259 punct ", ",result
R2302 T5269 T5259 cc but,result
R2303 T5270 T5271 det the,detectability
R2304 T5271 T5272 nsubjpass detectability,decreased
R2305 T5272 T5259 conj decreased,result
R2306 T5273 T5271 prep of,detectability
R2307 T5274 T5275 amod other,antigens
R2308 T5275 T5273 pobj antigens,of
R2309 T5276 T5272 aux might,decreased
R2310 T5277 T5272 auxpass be,decreased
R2311 T5278 T5272 punct .,decreased
R2312 T5280 T5281 advmod Moreover,has
R2313 T5282 T5281 punct ", ",has
R2314 T5283 T5284 det the,pre-treatment
R2315 T5284 T5281 nsubj pre-treatment,has
R2316 T5285 T5284 amod optimal,pre-treatment
R2317 T5286 T5287 aux to,individualised
R2318 T5287 T5281 xcomp individualised,has
R2319 T5288 T5287 auxpass be,individualised
R2320 T5289 T5287 prep for,individualised
R2321 T5290 T5291 det each,antigen
R2322 T5291 T5289 pobj antigen,for
R2323 T5292 T5281 punct .,has
R2324 T5294 T5295 aux To,verify
R2325 T5295 T5296 advcl verify,used
R2326 T5297 T5298 det the,presence
R2327 T5298 T5295 dobj presence,verify
R2328 T5299 T5298 prep of,presence
R2329 T5300 T5301 amod endogenous,A7
R2330 T5301 T5299 pobj A7,of
R2331 T5302 T5301 compound Annexin,A7
R2332 T5303 T5295 prep in,verify
R2333 T5304 T5303 pobj nuclei,in
R2334 T5305 T5296 punct ", ",used
R2335 T5306 T5296 nsubj we,used
R2336 T5307 T5308 det a,approach
R2337 T5308 T5296 dobj approach,used
R2338 T5309 T5308 amod biochemical,approach
R2339 T5310 T5296 cc and,used
R2340 T5311 T5296 conj purified,used
R2341 T5312 T5311 dobj nuclei,purified
R2342 T5313 T5311 prep from,purified
R2343 T5314 T5315 nmod neuro,cells
R2344 T5315 T5313 pobj cells,from
R2345 T5316 T5314 punct -,neuro
R2346 T5317 T5314 nummod 2a,neuro
R2347 T5318 T5314 punct ", ",neuro
R2348 T5319 T5314 conj PC,neuro
R2349 T5320 T5319 punct -,PC
R2350 T5321 T5319 nummod 12,PC
R2351 T5322 T5319 punct ", ",PC
R2352 T5323 T5319 cc and,PC
R2353 T5324 T5319 conj C6,PC
R2354 T5325 T5296 cc and,used
R2355 T5326 T5296 conj treated,used
R2356 T5327 T5326 dobj them,treated
R2357 T5328 T5326 prep with,treated
R2358 T5329 T5330 det a,buffer
R2359 T5330 T5328 pobj buffer,with
R2360 T5331 T5330 amod hypotonic,buffer
R2361 T5332 T5330 compound extraction,buffer
R2362 T5333 T5334 aux to,obtain
R2363 T5334 T5326 advcl obtain,treated
R2364 T5335 T5336 det the,nucleoplasm
R2365 T5336 T5334 dobj nucleoplasm,obtain
R2366 T5337 T5338 punct (,7A
R2367 T5338 T5326 parataxis 7A,treated
R2368 T5339 T5338 compound Fig.,7A
R2369 T5340 T5338 punct ),7A
R2370 T5341 T5296 punct .,used
R2371 T5343 T5344 det The,nucleoplasm
R2372 T5344 T5345 nsubjpass nucleoplasm,subjected
R2373 T5346 T5344 cc and,nucleoplasm
R2374 T5347 T5348 det the,nuclei
R2375 T5348 T5344 conj nuclei,nucleoplasm
R2376 T5349 T5348 amod remaining,nuclei
R2377 T5350 T5344 punct ", ",nucleoplasm
R2378 T5351 T5352 prep from,extracted
R2379 T5352 T5344 relcl extracted,nucleoplasm
R2380 T5353 T5351 pobj which,from
R2381 T5354 T5355 det the,nucleoplasm
R2382 T5355 T5352 nsubjpass nucleoplasm,extracted
R2383 T5356 T5352 aux has,extracted
R2384 T5357 T5352 auxpass been,extracted
R2385 T5358 T5352 advmod partially,extracted
R2386 T5359 T5345 punct ", ",subjected
R2387 T5360 T5345 auxpass were,subjected
R2388 T5361 T5345 prep to,subjected
R2389 T5362 T5363 compound SDS,PAGE
R2390 T5363 T5361 pobj PAGE,to
R2391 T5364 T5363 punct -,PAGE
R2392 T5365 T5363 cc and,PAGE
R2393 T5366 T5367 compound Western,blotting
R2394 T5367 T5363 conj blotting,PAGE
R2395 T5368 T5345 punct .,subjected
R2396 T5370 T5371 compound Western,blots
R2397 T5371 T5372 nsubj blots,showed
R2398 T5372 T5379 ccomp showed,extracted
R2399 T5373 T5371 acl probed,blots
R2400 T5374 T5373 prep with,probed
R2401 T5375 T5376 nmod mAb,203
R2402 T5376 T5374 pobj 203,with
R2403 T5377 T5378 punct –,217
R2404 T5378 T5376 prep 217,203
R2405 T5380 T5381 compound Annexin,A7
R2406 T5381 T5372 dobj A7,showed
R2407 T5382 T5372 prep in,showed
R2408 T5383 T5384 det the,nucleoplasm
R2409 T5384 T5382 pobj nucleoplasm,in
R2410 T5385 T5384 prep of,nucleoplasm
R2411 T5386 T5387 amod neuronal,lines
R2412 T5387 T5385 pobj lines,of
R2413 T5388 T5389 punct (,2a
R2414 T5389 T5386 parataxis 2a,neuronal
R2415 T5390 T5389 compound neuro,2a
R2416 T5391 T5389 punct -,2a
R2417 T5392 T5389 punct ", ",2a
R2418 T5393 T5389 appos PC,2a
R2419 T5394 T5393 punct -,PC
R2420 T5395 T5393 nummod 12,PC
R2421 T5396 T5389 punct ),2a
R2422 T5397 T5386 cc and,neuronal
R2423 T5398 T5386 conj astroglial,neuronal
R2424 T5399 T5400 punct (,C6
R2425 T5400 T5398 parataxis C6,astroglial
R2426 T5401 T5400 punct ),C6
R2427 T5402 T5387 compound cell,lines
R2428 T5403 T5404 punct (,7A
R2429 T5404 T5372 parataxis 7A,showed
R2430 T5405 T5404 compound Fig.,7A
R2431 T5406 T5404 punct ),7A
R2432 T5407 T5379 punct ", ",extracted
R2433 T5408 T5379 advmod however,extracted
R2434 T5409 T5379 punct ", ",extracted
R2435 T5410 T5411 det the,isoform
R2436 T5411 T5379 nsubjpass isoform,extracted
R2437 T5412 T5411 amod large,isoform
R2438 T5413 T5379 aux could,extracted
R2439 T5414 T5379 advmod only,extracted
R2440 T5415 T5379 auxpass be,extracted
R2441 T5416 T5379 advmod clearly,extracted
R2442 T5417 T5379 prep with,extracted
R2443 T5418 T5417 pobj nucleoplasm,with
R2444 T5419 T5418 prep from,nucleoplasm
R2445 T5420 T5421 compound C6,cells
R2446 T5421 T5419 pobj cells,from
R2447 T5422 T5379 punct .,extracted
R2448 T5424 T5425 prep For,used
R2449 T5426 T5424 pobj control,For
R2450 T5427 T5425 punct ", ",used
R2451 T5428 T5425 nsubjpass antibodies,used
R2452 T5429 T5428 prep against,antibodies
R2453 T5430 T5429 pobj LAP2α,against
R2454 T5431 T5430 punct ", ",LAP2α
R2455 T5432 T5430 conj Emerin,LAP2α
R2456 T5433 T5432 cc and,Emerin
R2457 T5434 T5432 conj tubulin,Emerin
R2458 T5435 T5425 auxpass were,used
R2459 T5436 T5437 aux to,verify
R2460 T5437 T5425 advcl verify,used
R2461 T5438 T5439 mark that,extracted
R2462 T5439 T5437 ccomp extracted,verify
R2463 T5440 T5441 det the,nucleoplasm
R2464 T5441 T5439 nsubjpass nucleoplasm,extracted
R2465 T5442 T5443 punct (,LAP2α
R2466 T5443 T5441 parataxis LAP2α,nucleoplasm
R2467 T5444 T5443 dep marker,LAP2α
R2468 T5445 T5443 punct :,LAP2α
R2469 T5446 T5443 punct ", ",LAP2α
R2470 T5447 T5448 dep which,is
R2471 T5448 T5443 relcl is,LAP2α
R2472 T5449 T5450 compound anon,membrane
R2473 T5450 T5452 npadvmod membrane,bound
R2474 T5451 T5450 punct -,membrane
R2475 T5452 T5454 amod bound,isoform
R2476 T5453 T5452 punct -,bound
R2477 T5454 T5448 attr isoform,is
R2478 T5455 T5454 prep of,isoform
R2479 T5456 T5455 pobj LAP2,of
R2480 T5457 T5443 punct ),LAP2α
R2481 T5458 T5439 auxpass was,extracted
R2482 T5459 T5439 advmod successfully,extracted
R2483 T5460 T5439 cc and,extracted
R2484 T5461 T5460 advmod also,and
R2485 T5462 T5463 neg not,contaminated
R2486 T5463 T5439 conj contaminated,extracted
R2487 T5464 T5463 prep by,contaminated
R2488 T5465 T5466 amod nuclear,membranes
R2489 T5466 T5464 pobj membranes,by
R2490 T5467 T5468 punct (,Emerin
R2491 T5468 T5466 parataxis Emerin,membranes
R2492 T5469 T5468 dep marker,Emerin
R2493 T5470 T5468 punct :,Emerin
R2494 T5471 T5468 punct ),Emerin
R2495 T5472 T5464 cc or,by
R2496 T5473 T5464 conj by,by
R2497 T5474 T5473 pobj cytoplasm,by
R2498 T5475 T5476 punct (,tubulin
R2499 T5476 T5474 parataxis tubulin,cytoplasm
R2500 T5477 T5476 dep marker,tubulin
R2501 T5478 T5476 punct :,tubulin
R2502 T5479 T5476 punct ),tubulin
R2503 T5480 T5425 punct .,used
R2504 T5482 T5483 advmod Likewise,observed
R2505 T5484 T5483 punct ", ",observed
R2506 T5485 T5483 prep in,observed
R2507 T5486 T5487 det these,lines
R2508 T5487 T5485 pobj lines,in
R2509 T5488 T5487 amod neuronal,lines
R2510 T5489 T5488 cc and,neuronal
R2511 T5490 T5488 conj astroglial,neuronal
R2512 T5491 T5487 compound cell,lines
R2513 T5492 T5493 compound Annexin,A7
R2514 T5493 T5483 nsubjpass A7,observed
R2515 T5494 T5483 aux had,observed
R2516 T5495 T5483 auxpass been,observed
R2517 T5496 T5483 prep in,observed
R2518 T5497 T5498 det the,nucleus
R2519 T5498 T5496 pobj nucleus,in
R2520 T5499 T5483 prep by,observed
R2521 T5500 T5499 pobj immunofluorescence,by
R2522 T5501 T5502 punct (,7B
R2523 T5502 T5483 parataxis 7B,observed
R2524 T5503 T5502 compound Fig.,7B
R2525 T5504 T5502 cc and,7B
R2526 T5505 T5506 nsubj data,shown
R2527 T5506 T5502 conj shown,7B
R2528 T5507 T5506 neg not,shown
R2529 T5508 T5506 punct ),shown
R2530 T5509 T5483 punct .,observed
R2531 T5511 T5512 nsubj We,noticed
R2532 T5512 T5513 ccomp noticed,found
R2533 T5514 T5515 det no,difference
R2534 T5515 T5512 dobj difference,noticed
R2535 T5516 T5515 prep between,difference
R2536 T5517 T5518 nmod PC,cells
R2537 T5518 T5516 pobj cells,between
R2538 T5519 T5517 punct -,PC
R2539 T5520 T5517 nummod 12,PC
R2540 T5521 T5517 cc and,PC
R2541 T5522 T5523 compound neuro,2a
R2542 T5523 T5517 conj 2a,PC
R2543 T5524 T5523 punct -,2a
R2544 T5525 T5513 punct ", ",found
R2545 T5526 T5513 advmod however,found
R2546 T5527 T5513 punct ", ",found
R2547 T5528 T5513 prep as,found
R2548 T5529 T5528 prep for,as
R2549 T5530 T5531 det the,sections
R2550 T5531 T5529 pobj sections,for
R2551 T5532 T5531 compound brain,sections
R2552 T5533 T5513 punct ", ",found
R2553 T5534 T5513 nsubj we,found
R2554 T5535 T5536 det an,increase
R2555 T5536 T5513 dobj increase,found
R2556 T5537 T5536 prep of,increase
R2557 T5538 T5539 det the,staining
R2558 T5539 T5537 pobj staining,of
R2559 T5540 T5541 compound Annexin,A7
R2560 T5541 T5539 compound A7,staining
R2561 T5542 T5513 prep after,found
R2562 T5543 T5542 pobj pre-treatment,after
R2563 T5544 T5543 prep with,pre-treatment
R2564 T5545 T5544 pobj trypsin,with
R2565 T5546 T5513 punct .,found
R6997 T14995 T14996 amod Nuclear,localization
R6998 T14997 T14996 prep of,localization
R6999 T14998 T14999 compound Annexin,A7
R7000 T14999 T14997 pobj A7,of
R7001 T15000 T14996 punct .,localization
R7002 T15002 T15003 punct (,A
R7003 T15003 T15004 meta A,Extraction
R7004 T15005 T15003 punct ),A
R7005 T15006 T15004 prep of,Extraction
R7006 T15007 T15008 compound Annexin,A7
R7007 T15008 T15006 pobj A7,of
R7008 T15009 T15004 prep from,Extraction
R7009 T15010 T15009 pobj nuclei,from
R7010 T15011 T15010 prep of,nuclei
R7011 T15012 T15011 pobj neuronal,of
R7012 T15013 T15014 punct (,PC
R7013 T15014 T15012 parataxis PC,neuronal
R7014 T15015 T15014 punct -,PC
R7015 T15016 T15014 nummod 12,PC
R7016 T15017 T15014 punct ", ",PC
R7017 T15018 T15014 appos N2A,PC
R7018 T15019 T15014 punct ),PC
R7019 T15020 T15012 cc and,neuronal
R7020 T15021 T15022 det an,astroglial
R7021 T15022 T15023 nmod astroglial,line
R7022 T15023 T15012 conj line,neuronal
R7023 T15024 T15025 punct (,C6
R7024 T15025 T15022 parataxis C6,astroglial
R7025 T15026 T15025 punct ),C6
R7026 T15027 T15023 compound cell,line
R7027 T15028 T15004 acl using,Extraction
R7028 T15029 T15030 det a,buffer
R7029 T15030 T15028 dobj buffer,using
R7030 T15031 T15030 amod hypotonic,buffer
R7031 T15032 T15004 punct .,Extraction
R7032 T15034 T15035 nsubjpass Samples,subjected
R7033 T15036 T15034 prep of,Samples
R7034 T15037 T15038 amod total,cells
R7035 T15038 T15036 pobj cells,of
R7036 T15039 T15040 punct (,c
R7037 T15040 T15034 parataxis c,Samples
R7038 T15041 T15040 punct ),c
R7039 T15042 T15034 punct ", ",Samples
R7040 T15043 T15034 cc and,Samples
R7041 T15044 T15045 amod corresponding,amounts
R7042 T15045 T15034 conj amounts,Samples
R7043 T15046 T15045 prep of,amounts
R7044 T15047 T15048 amod nuclear,membranes
R7045 T15048 T15046 pobj membranes,of
R7046 T15049 T15050 punct (,m
R7047 T15050 T15048 parataxis m,membranes
R7048 T15051 T15050 punct ),m
R7049 T15052 T15048 cc and,membranes
R7050 T15053 T15048 conj nucleoplasm,membranes
R7051 T15054 T15055 punct (,p
R7052 T15055 T15053 parataxis p,nucleoplasm
R7053 T15056 T15055 punct ),p
R7054 T15057 T15035 auxpass were,subjected
R7055 T15058 T15035 prep to,subjected
R7056 T15059 T15060 compound SDS,PAGE
R7057 T15060 T15058 pobj PAGE,to
R7058 T15061 T15060 punct -,PAGE
R7059 T15062 T15060 cc and,PAGE
R7060 T15063 T15064 compound Western,blotting
R7061 T15064 T15060 conj blotting,PAGE
R7062 T15065 T15035 punct .,subjected
R7063 T15067 T15068 nmod Annexin,A7
R7064 T15068 T15069 nmod A7,isoforms
R7065 T15069 T15073 nsubj isoforms,are
R7066 T15070 T15068 punct (,A7
R7067 T15071 T15068 appos AnxA7,A7
R7068 T15072 T15069 punct ),isoforms
R7069 T15074 T15073 acomp extractable,are
R7070 T15075 T15074 prep from,extractable
R7071 T15076 T15077 det the,nucleus
R7072 T15077 T15075 pobj nucleus,from
R7073 T15078 T15077 prep of,nucleus
R7074 T15079 T15080 det the,cells
R7075 T15080 T15078 pobj cells,of
R7076 T15081 T15080 amod indicated,cells
R7077 T15082 T15083 punct (,arrowhead
R7078 T15083 T15080 parataxis arrowhead,cells
R7079 T15084 T15085 nummod 47,kDa
R7080 T15085 T15086 nmod kDa,isoform
R7081 T15086 T15083 dep isoform,arrowhead
R7082 T15087 T15085 cc and,kDa
R7083 T15088 T15089 nummod 51,kDa
R7084 T15089 T15085 conj kDa,kDa
R7085 T15090 T15083 punct ", ",arrowhead
R7086 T15091 T15083 punct ),arrowhead
R7087 T15092 T15073 punct .,are
R7088 T15094 T15095 prep For,shown
R7089 T15096 T15094 pobj control,For
R7090 T15097 T15095 punct ", ",shown
R7091 T15098 T15095 nsubjpass immunoblotting,shown
R7092 T15099 T15098 prep of,immunoblotting
R7093 T15100 T15099 pobj LAP2α,of
R7094 T15101 T15102 punct (,isoform
R7095 T15102 T15100 parataxis isoform,LAP2α
R7096 T15103 T15104 nummod 75,kDa
R7097 T15104 T15102 dep kDa,isoform
R7098 T15105 T15102 punct ;,isoform
R7099 T15106 T15107 amod non-membrane,bound
R7100 T15107 T15102 amod bound,isoform
R7101 T15108 T15107 punct -,bound
R7102 T15109 T15102 punct ),isoform
R7103 T15110 T15100 punct ", ",LAP2α
R7104 T15111 T15100 conj Emerin,LAP2α
R7105 T15112 T15111 punct (,Emerin
R7106 T15113 T15111 appos EM,Emerin
R7107 T15114 T15115 punct ", ",kDa
R7108 T15115 T15111 parataxis kDa,Emerin
R7109 T15116 T15115 nummod 34,kDa
R7110 T15117 T15115 punct ),kDa
R7111 T15118 T15111 punct ", ",Emerin
R7112 T15119 T15111 cc and,Emerin
R7113 T15120 T15111 conj tubulin,Emerin
R7114 T15121 T15120 punct (,tubulin
R7115 T15122 T15120 appos TB,tubulin
R7116 T15123 T15124 punct ", ",kDa
R7117 T15124 T15120 parataxis kDa,tubulin
R7118 T15125 T15124 nummod 55,kDa
R7119 T15126 T15124 punct ),kDa
R7120 T15127 T15128 dep which,are
R7121 T15128 T15100 relcl are,LAP2α
R7122 T15129 T15128 acomp specific,are
R7123 T15130 T15129 prep for,specific
R7124 T15131 T15130 pobj nucleoplasm,for
R7125 T15132 T15133 punct (,LAP2α
R7126 T15133 T15131 parataxis LAP2α,nucleoplasm
R7127 T15134 T15133 punct ),LAP2α
R7128 T15135 T15100 punct ", ",LAP2α
R7129 T15136 T15137 amod nuclear,membranes
R7130 T15137 T15100 appos membranes,LAP2α
R7131 T15138 T15139 punct (,Emerin
R7132 T15139 T15137 parataxis Emerin,membranes
R7133 T15140 T15139 punct ),Emerin
R7134 T15141 T15137 punct ", ",membranes
R7135 T15142 T15137 cc and,membranes
R7136 T15143 T15144 det the,cytoplasm
R7137 T15144 T15137 conj cytoplasm,membranes
R7138 T15145 T15146 punct (,tubulin
R7139 T15146 T15144 parataxis tubulin,cytoplasm
R7140 T15147 T15146 punct ),tubulin
R7141 T15148 T15095 auxpass are,shown
R7142 T15149 T15095 punct .,shown
R7143 T15152 T15153 mark that,is
R7144 T15153 T15151 ccomp is,Note
R7145 T15154 T15155 det the,nucleoplasm
R7146 T15155 T15153 nsubj nucleoplasm,is
R7147 T15156 T15157 advmod only,partially
R7148 T15157 T15158 advmod partially,extractable
R7149 T15158 T15153 acomp extractable,is
R7150 T15159 T15151 punct .,Note
R7151 T15161 T15162 punct (,B
R7152 T15162 T15163 meta B,images
R7153 T15164 T15162 punct ),B
R7154 T15165 T15163 compound Immunofluorescence,images
R7155 T15166 T15163 prep of,images
R7156 T15167 T15168 det the,cells
R7157 T15168 T15166 pobj cells,of
R7158 T15169 T15168 nmod PC,cells
R7159 T15170 T15169 punct -,PC
R7160 T15171 T15169 nummod 12,PC
R7161 T15172 T15168 acl used,cells
R7162 T15173 T15163 punct .,images
R7163 T15175 T15176 nsubjpass Cells,fixed
R7164 T15177 T15176 auxpass were,fixed
R7165 T15178 T15176 prep with,fixed
R7166 T15179 T15178 pobj paraformaldehyde,with
R7167 T15180 T15176 punct ", ",fixed
R7168 T15181 T15176 conj permeabilized,fixed
R7169 T15182 T15181 prep with,permeabilized
R7170 T15183 T15184 compound Triton,X
R7171 T15184 T15182 pobj X,with
R7172 T15185 T15184 punct -,X
R7173 T15186 T15184 nummod 100,X
R7174 T15187 T15181 punct ", ",permeabilized
R7175 T15188 T15181 conj pretreated,permeabilized
R7176 T15189 T15188 prep with,pretreated
R7177 T15190 T15189 pobj trypsin,with
R7178 T15191 T15192 mark as,indicated
R7179 T15192 T15188 advcl indicated,pretreated
R7180 T15193 T15188 punct ", ",pretreated
R7181 T15194 T15188 cc and,pretreated
R7182 T15195 T15188 conj stained,pretreated
R7183 T15196 T15195 prep with,stained
R7184 T15197 T15198 compound Annexin,A7
R7185 T15198 T15199 npadvmod A7,specific
R7186 T15199 T15200 amod specific,203
R7187 T15200 T15196 pobj 203,with
R7188 T15201 T15200 nmod mAb,203
R7189 T15202 T15203 punct –,217
R7190 T15203 T15200 prep 217,203
R7191 T15204 T15205 punct ", ",μm
R7192 T15205 T15195 parataxis μm,stained
R7193 T15206 T15205 dep DAPI,μm
R7194 T15207 T15206 prep in,DAPI
R7195 T15208 T15207 amod blue,in
R7196 T15209 T15205 punct ", ",μm
R7197 T15210 T15205 dep bar,μm
R7198 T15211 T15205 nummod 10,μm
R7199 T15212 T15176 punct .,fixed