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PMC:1084331 / 39085-47036 JSONTXT 6 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T12345 23-33 NN denotes Generation
T12346 34-36 IN denotes of
T12347 37-47 JJ denotes transgenic
T12348 48-52 NNS denotes mice
T12349 53-56 CC denotes and
T12350 57-62 NN denotes mouse
T12351 63-71 NN denotes genetics
T12352 71-192 sentence denotes Er81EWS-Pea3 mice were generated following a strategy similar to that described for the generation of Er81NLZ mice [14].
T12353 72-79 NN denotes Er81EWS
T12355 79-80 HYPH denotes -
T12354 80-84 NN denotes Pea3
T12356 85-89 NNS denotes mice
T12358 90-94 VBD denotes were
T12357 95-104 VBN denotes generated
T12359 105-114 VBG denotes following
T12360 115-116 DT denotes a
T12361 117-125 NN denotes strategy
T12362 126-133 JJ denotes similar
T12363 134-136 IN denotes to
T12364 137-141 DT denotes that
T12365 142-151 VBN denotes described
T12366 152-155 IN denotes for
T12367 156-159 DT denotes the
T12368 160-170 NN denotes generation
T12369 171-173 IN denotes of
T12370 174-181 NN denotes Er81NLZ
T12371 182-186 NNS denotes mice
T12372 187-188 -LRB- denotes [
T12373 188-190 CD denotes 14
T12374 190-191 -RRB- denotes ]
T12375 191-192 . denotes .
T12376 192-398 sentence denotes In brief, a targeting vector with a cDNA coding for EWS-Pea3 was inserted in frame with the endogenous start ATG into exon 2 of the Er81 genomic locus and used for homologous recombination in W95 ES cells.
T12377 193-195 IN denotes In
T12379 196-201 NN denotes brief
T12380 201-203 , denotes ,
T12381 203-204 DT denotes a
T12383 205-214 NN denotes targeting
T12382 215-221 NN denotes vector
T12384 222-226 IN denotes with
T12385 227-228 DT denotes a
T12387 229-233 NN denotes cDNA
T12386 234-240 NN denotes coding
T12388 241-244 IN denotes for
T12389 245-248 NN denotes EWS
T12391 248-249 HYPH denotes -
T12390 249-253 NN denotes Pea3
T12392 254-257 VBD denotes was
T12378 258-266 VBN denotes inserted
T12393 267-269 IN denotes in
T12394 270-275 NN denotes frame
T12395 276-280 IN denotes with
T12396 281-284 DT denotes the
T12398 285-295 JJ denotes endogenous
T12399 296-301 NN denotes start
T12397 302-305 NN denotes ATG
T12400 306-310 IN denotes into
T12401 311-315 NN denotes exon
T12402 316-317 CD denotes 2
T12403 318-320 IN denotes of
T12404 321-324 DT denotes the
T12406 325-329 NN denotes Er81
T12407 330-337 JJ denotes genomic
T12405 338-343 NN denotes locus
T12408 344-347 CC denotes and
T12409 348-352 VBN denotes used
T12410 353-356 IN denotes for
T12411 357-367 JJ denotes homologous
T12412 368-381 NN denotes recombination
T12413 382-384 IN denotes in
T12414 385-388 NN denotes W95
T12416 389-391 NN denotes ES
T12415 392-397 NNS denotes cells
T12417 397-398 . denotes .
T12418 398-499 sentence denotes EWS-Pea3 represents a fusion gene between the amino terminal of EWS and the ETS domain of Pea3 [20].
T12419 399-402 NN denotes EWS
T12421 402-403 HYPH denotes -
T12420 403-407 NN denotes Pea3
T12422 408-418 VBZ denotes represents
T12423 419-420 DT denotes a
T12425 421-427 NN denotes fusion
T12424 428-432 NN denotes gene
T12426 433-440 IN denotes between
T12427 441-444 DT denotes the
T12429 445-450 NN denotes amino
T12428 451-459 NN denotes terminal
T12430 460-462 IN denotes of
T12431 463-466 NN denotes EWS
T12432 467-470 CC denotes and
T12433 471-474 DT denotes the
T12435 475-478 NN denotes ETS
T12434 479-485 NN denotes domain
T12436 486-488 IN denotes of
T12437 489-493 NN denotes Pea3
T12438 494-495 -LRB- denotes [
T12439 495-497 CD denotes 20
T12440 497-498 -RRB- denotes ]
T12441 498-499 . denotes .
T12442 499-635 sentence denotes The primer pair used to specifically detect the Er81EWS-Pea3 allele was 5′- CAGCCACTGCACCTACAAGAC-3′ and 5′- CTTCCTGCTTGATGTCTCCTTC-3′.
T12443 500-503 DT denotes The
T12445 504-510 NN denotes primer
T12444 511-515 NN denotes pair
T12447 516-520 VBN denotes used
T12448 521-523 TO denotes to
T12450 524-536 RB denotes specifically
T12449 537-543 VB denotes detect
T12451 544-547 DT denotes the
T12453 548-555 NN denotes Er81EWS
T12455 555-556 HYPH denotes -
T12454 556-560 NN denotes Pea3
T12452 561-567 NN denotes allele
T12446 568-571 VBD denotes was
T12456 572-573 CD denotes 5
T12458 573-574 SYM denotes ′
T12459 574-575 HYPH denotes -
T12457 576-597 NN denotes CAGCCACTGCACCTACAAGAC
T12460 597-598 HYPH denotes -
T12461 598-599 CD denotes 3
T12462 599-600 SYM denotes ′
T12463 601-604 CC denotes and
T12464 605-606 CD denotes 5
T12466 606-607 SYM denotes ′
T12467 607-608 HYPH denotes -
T12465 609-631 NN denotes CTTCCTGCTTGATGTCTCCTTC
T12468 631-632 HYPH denotes -
T12469 632-633 CD denotes 3
T12470 633-634 SYM denotes ′
T12471 634-635 . denotes .
T12472 635-935 sentence denotes For the generation of TaumGFP and TauEWS-Pea3 mice, lox-STOP-lox-mGFP-IRES-NLS-LacZ-pA and lox-STOP-lox-EWS-Pea3-IRES-NLS-LacZ-pA targeting cassettes were integrated into exon 2 of the Tau genomic locus (the endogenous start ATG was removed in the targeting vectors; details available upon request).
T12473 636-639 IN denotes For
T12475 640-643 DT denotes the
T12476 644-654 NN denotes generation
T12477 655-657 IN denotes of
T12478 658-665 NN denotes TaumGFP
T12480 666-669 CC denotes and
T12481 670-676 NN denotes TauEWS
T12483 676-677 HYPH denotes -
T12482 677-681 NN denotes Pea3
T12479 682-686 NNS denotes mice
T12484 686-688 , denotes ,
T12485 688-691 NN denotes lox
T12487 691-692 HYPH denotes -
T12488 692-696 NN denotes STOP
T12489 696-697 HYPH denotes -
T12490 697-700 NN denotes lox
T12491 700-701 HYPH denotes -
T12492 701-705 NN denotes mGFP
T12493 705-706 HYPH denotes -
T12494 706-710 NN denotes IRES
T12495 710-711 HYPH denotes -
T12496 711-714 NN denotes NLS
T12497 714-715 HYPH denotes -
T12498 715-719 NN denotes LacZ
T12499 719-720 HYPH denotes -
T12486 720-722 NN denotes pA
T12501 723-726 CC denotes and
T12502 727-730 NN denotes lox
T12504 730-731 HYPH denotes -
T12505 731-735 NN denotes STOP
T12506 735-736 HYPH denotes -
T12507 736-739 NN denotes lox
T12508 739-740 HYPH denotes -
T12509 740-743 NN denotes EWS
T12510 743-744 HYPH denotes -
T12511 744-748 NN denotes Pea3
T12512 748-749 HYPH denotes -
T12513 749-753 NN denotes IRES
T12514 753-754 HYPH denotes -
T12515 754-757 NN denotes NLS
T12516 757-758 HYPH denotes -
T12517 758-762 NN denotes LacZ
T12518 762-763 HYPH denotes -
T12503 763-765 NN denotes pA
T12519 766-775 NN denotes targeting
T12500 776-785 NNS denotes cassettes
T12520 786-790 VBD denotes were
T12474 791-801 VBN denotes integrated
T12521 802-806 IN denotes into
T12522 807-811 NN denotes exon
T12523 812-813 CD denotes 2
T12524 814-816 IN denotes of
T12525 817-820 DT denotes the
T12527 821-824 NN denotes Tau
T12528 825-832 JJ denotes genomic
T12526 833-838 NN denotes locus
T12529 839-840 -LRB- denotes (
T12531 840-843 DT denotes the
T12533 844-854 JJ denotes endogenous
T12534 855-860 NN denotes start
T12532 861-864 NN denotes ATG
T12535 865-868 VBD denotes was
T12530 869-876 VBN denotes removed
T12536 877-879 IN denotes in
T12537 880-883 DT denotes the
T12539 884-893 NN denotes targeting
T12538 894-901 NNS denotes vectors
T12540 901-902 : denotes ;
T12541 903-910 NNS denotes details
T12542 911-920 JJ denotes available
T12543 921-925 IN denotes upon
T12544 926-933 NN denotes request
T12545 933-934 -RRB- denotes )
T12546 934-935 . denotes .
T12547 935-972 sentence denotes mGFP was provided by P. Caroni [25].
T12548 936-940 NN denotes mGFP
T12550 941-944 VBD denotes was
T12549 945-953 VBN denotes provided
T12551 954-956 IN denotes by
T12552 957-959 NNP denotes P.
T12553 960-966 NNP denotes Caroni
T12554 967-968 -LRB- denotes [
T12555 968-970 CD denotes 25
T12556 970-971 -RRB- denotes ]
T12557 971-972 . denotes .
T12558 972-1096 sentence denotes ES cell recombinants were screened by Southern blot analysis using the probe in the 5′ region as described previously [41].
T12559 973-975 NN denotes ES
T12561 976-980 NN denotes cell
T12560 981-993 NNS denotes recombinants
T12563 994-998 VBD denotes were
T12562 999-1007 VBN denotes screened
T12564 1008-1010 IN denotes by
T12565 1011-1019 NNP denotes Southern
T12566 1020-1024 NN denotes blot
T12567 1025-1033 NN denotes analysis
T12568 1034-1039 VBG denotes using
T12569 1040-1043 DT denotes the
T12570 1044-1049 NN denotes probe
T12571 1050-1052 IN denotes in
T12572 1053-1056 DT denotes the
T12574 1057-1058 CD denotes 5
T12575 1058-1059 SYM denotes ′
T12573 1060-1066 NN denotes region
T12576 1067-1069 IN denotes as
T12577 1070-1079 VBN denotes described
T12578 1080-1090 RB denotes previously
T12579 1091-1092 -LRB- denotes [
T12580 1092-1094 CD denotes 41
T12581 1094-1095 -RRB- denotes ]
T12582 1095-1096 . denotes .
T12583 1096-1190 sentence denotes Frequency of recombination in 129/Ola ES cells was approximately 1/3 for both Tau constructs.
T12584 1097-1106 NN denotes Frequency
T12586 1107-1109 IN denotes of
T12587 1110-1123 NN denotes recombination
T12588 1124-1126 IN denotes in
T12589 1127-1130 CD denotes 129
T12591 1130-1131 HYPH denotes /
T12590 1131-1134 NN denotes Ola
T12593 1135-1137 NN denotes ES
T12592 1138-1143 NNS denotes cells
T12585 1144-1147 VBD denotes was
T12594 1148-1161 RB denotes approximately
T12596 1162-1163 CD denotes 1
T12597 1163-1164 SYM denotes /
T12595 1164-1165 CD denotes 3
T12598 1166-1169 IN denotes for
T12599 1170-1174 DT denotes both
T12601 1175-1178 NN denotes Tau
T12600 1179-1189 NNS denotes constructs
T12602 1189-1190 . denotes .
T12603 1190-1345 sentence denotes For the generation of PVCre mice, mouse genomic clones were obtained by screening a 129SV/J genomic library (Incyte, Wilmington, Delaware, United States).
T12604 1191-1194 IN denotes For
T12606 1195-1198 DT denotes the
T12607 1199-1209 NN denotes generation
T12608 1210-1212 IN denotes of
T12609 1213-1218 NN denotes PVCre
T12610 1219-1223 NNS denotes mice
T12611 1223-1225 , denotes ,
T12612 1225-1230 NN denotes mouse
T12614 1231-1238 JJ denotes genomic
T12613 1239-1245 NNS denotes clones
T12615 1246-1250 VBD denotes were
T12605 1251-1259 VBN denotes obtained
T12616 1260-1262 IN denotes by
T12617 1263-1272 VBG denotes screening
T12618 1273-1274 DT denotes a
T12620 1275-1280 NN denotes 129SV
T12622 1280-1281 HYPH denotes /
T12621 1281-1282 NN denotes J
T12623 1283-1290 JJ denotes genomic
T12619 1291-1298 NN denotes library
T12624 1299-1300 -LRB- denotes (
T12625 1300-1306 NNP denotes Incyte
T12626 1306-1308 , denotes ,
T12627 1308-1318 NNP denotes Wilmington
T12628 1318-1320 , denotes ,
T12629 1320-1328 NNP denotes Delaware
T12630 1328-1330 , denotes ,
T12631 1330-1336 NNP denotes United
T12632 1337-1343 NNP denotes States
T12633 1343-1344 -RRB- denotes )
T12634 1344-1345 . denotes .
T12635 1345-1414 sentence denotes For details on the genomic structure of the mouse PV locus see [42].
T12636 1346-1349 IN denotes For
T12638 1350-1357 NNS denotes details
T12639 1358-1360 IN denotes on
T12640 1361-1364 DT denotes the
T12642 1365-1372 JJ denotes genomic
T12641 1373-1382 NN denotes structure
T12643 1383-1385 IN denotes of
T12644 1386-1389 DT denotes the
T12646 1390-1395 NN denotes mouse
T12647 1396-1398 NN denotes PV
T12645 1399-1404 NN denotes locus
T12637 1405-1408 VBP denotes see
T12648 1409-1410 -LRB- denotes [
T12649 1410-1412 CD denotes 42
T12650 1412-1413 -RRB- denotes ]
T12651 1413-1414 . denotes .
T12652 1414-1658 sentence denotes An IRES-Cre-pA targeting cassette [33] was integrated into the 3′ UTR of exon 5, and ES cell recombinants were screened with a 5′ probe (oligos, 5′- GAGATGACCCAGCCAGGATGCCTC-3′ and 5′- CTGACCACTCTCGCTCCGGTGTCC-3′; genomic DNA, HindIII digest).
T12653 1415-1417 DT denotes An
T12655 1418-1422 NN denotes IRES
T12657 1422-1423 HYPH denotes -
T12658 1423-1426 NN denotes Cre
T12659 1426-1427 HYPH denotes -
T12656 1427-1429 NN denotes pA
T12660 1430-1439 NN denotes targeting
T12654 1440-1448 NN denotes cassette
T12662 1449-1450 -LRB- denotes [
T12663 1450-1452 CD denotes 33
T12664 1452-1453 -RRB- denotes ]
T12665 1454-1457 VBD denotes was
T12661 1458-1468 VBN denotes integrated
T12666 1469-1473 IN denotes into
T12667 1474-1477 DT denotes the
T12669 1478-1479 CD denotes 3
T12670 1479-1480 SYM denotes ′
T12668 1481-1484 NN denotes UTR
T12671 1485-1487 IN denotes of
T12672 1488-1492 NN denotes exon
T12673 1493-1494 CD denotes 5
T12674 1494-1496 , denotes ,
T12675 1496-1499 CC denotes and
T12676 1500-1502 NN denotes ES
T12678 1503-1507 NN denotes cell
T12677 1508-1520 NNS denotes recombinants
T12680 1521-1525 VBD denotes were
T12679 1526-1534 VBN denotes screened
T12681 1535-1539 IN denotes with
T12682 1540-1541 DT denotes a
T12684 1542-1543 CD denotes 5
T12685 1543-1544 SYM denotes ′
T12683 1545-1550 NN denotes probe
T12686 1551-1552 -LRB- denotes (
T12688 1552-1558 NNS denotes oligos
T12689 1558-1560 , denotes ,
T12690 1560-1561 CD denotes 5
T12692 1561-1562 SYM denotes ′
T12693 1562-1563 HYPH denotes -
T12691 1564-1588 NN denotes GAGATGACCCAGCCAGGATGCCTC
T12694 1588-1589 HYPH denotes -
T12695 1589-1590 CD denotes 3
T12696 1590-1591 SYM denotes ′
T12697 1592-1595 CC denotes and
T12698 1596-1597 CD denotes 5
T12700 1597-1598 SYM denotes ′
T12701 1598-1599 HYPH denotes -
T12699 1600-1624 NN denotes CTGACCACTCTCGCTCCGGTGTCC
T12702 1624-1625 HYPH denotes -
T12703 1625-1626 CD denotes 3
T12704 1626-1627 SYM denotes ′
T12705 1627-1628 : denotes ;
T12706 1629-1636 JJ denotes genomic
T12707 1637-1640 NN denotes DNA
T12708 1640-1642 , denotes ,
T12709 1642-1649 NN denotes HindIII
T12687 1650-1656 NN denotes digest
T12710 1656-1657 -RRB- denotes )
T12711 1657-1658 . denotes .
T12712 1658-1733 sentence denotes The frequency of recombination in 129/Ola ES cells was approximately 1/20.
T12713 1659-1662 DT denotes The
T12714 1663-1672 NN denotes frequency
T12716 1673-1675 IN denotes of
T12717 1676-1689 NN denotes recombination
T12718 1690-1692 IN denotes in
T12719 1693-1696 CD denotes 129
T12721 1696-1697 HYPH denotes /
T12720 1697-1700 NN denotes Ola
T12723 1701-1703 NN denotes ES
T12722 1704-1709 NNS denotes cells
T12715 1710-1713 VBD denotes was
T12724 1714-1727 RB denotes approximately
T12726 1728-1729 CD denotes 1
T12727 1729-1730 SYM denotes /
T12725 1730-1732 CD denotes 20
T12728 1732-1733 . denotes .
T12729 1733-1865 sentence denotes Recombinant clones were aggregated with morula stage embryos to generate chimeric founder mice that transmitted the mutant alleles.
T12730 1734-1745 JJ denotes Recombinant
T12731 1746-1752 NNS denotes clones
T12733 1753-1757 VBD denotes were
T12732 1758-1768 VBN denotes aggregated
T12734 1769-1773 IN denotes with
T12735 1774-1780 NN denotes morula
T12736 1781-1786 NN denotes stage
T12737 1787-1794 NNS denotes embryos
T12738 1795-1797 TO denotes to
T12739 1798-1806 VB denotes generate
T12740 1807-1815 JJ denotes chimeric
T12742 1816-1823 NN denotes founder
T12741 1824-1828 NNS denotes mice
T12743 1829-1833 WDT denotes that
T12744 1834-1845 VBD denotes transmitted
T12745 1846-1849 DT denotes the
T12747 1850-1856 NN denotes mutant
T12746 1857-1864 NNS denotes alleles
T12748 1864-1865 . denotes .
T12749 1865-1972 sentence denotes In all experiments performed in this study, animals were of mixed genetic background (129/Ola and C57Bl6).
T12750 1866-1868 IN denotes In
T12752 1869-1872 DT denotes all
T12753 1873-1884 NNS denotes experiments
T12754 1885-1894 VBN denotes performed
T12755 1895-1897 IN denotes in
T12756 1898-1902 DT denotes this
T12757 1903-1908 NN denotes study
T12758 1908-1910 , denotes ,
T12759 1910-1917 NNS denotes animals
T12751 1918-1922 VBD denotes were
T12760 1923-1925 IN denotes of
T12761 1926-1931 JJ denotes mixed
T12763 1932-1939 JJ denotes genetic
T12762 1940-1950 NN denotes background
T12764 1951-1952 -LRB- denotes (
T12766 1952-1955 CD denotes 129
T12767 1955-1956 HYPH denotes /
T12765 1956-1959 NN denotes Ola
T12768 1960-1963 CC denotes and
T12769 1964-1970 NN denotes C57Bl6
T12770 1970-1971 -RRB- denotes )
T12771 1971-1972 . denotes .
T12772 1972-2138 sentence denotes Thy1spGFP transgenic mice were generated in analogy to De Paola et al. [25], and for these experiments a strain of mice with early embryonic expression was selected.
T12773 1973-1982 NN denotes Thy1spGFP
T12775 1983-1993 JJ denotes transgenic
T12774 1994-1998 NNS denotes mice
T12777 1999-2003 VBD denotes were
T12776 2004-2013 VBN denotes generated
T12778 2014-2016 IN denotes in
T12779 2017-2024 NN denotes analogy
T12780 2025-2027 IN denotes to
T12781 2028-2030 NNP denotes De
T12782 2031-2036 NNP denotes Paola
T12783 2037-2039 FW denotes et
T12784 2040-2043 FW denotes al.
T12785 2044-2045 -LRB- denotes [
T12786 2045-2047 CD denotes 25
T12787 2047-2048 -RRB- denotes ]
T12788 2048-2050 , denotes ,
T12789 2050-2053 CC denotes and
T12790 2054-2057 IN denotes for
T12792 2058-2063 DT denotes these
T12793 2064-2075 NNS denotes experiments
T12794 2076-2077 DT denotes a
T12795 2078-2084 NN denotes strain
T12796 2085-2087 IN denotes of
T12797 2088-2092 NNS denotes mice
T12798 2093-2097 IN denotes with
T12799 2098-2103 JJ denotes early
T12801 2104-2113 JJ denotes embryonic
T12800 2114-2124 NN denotes expression
T12802 2125-2128 VBD denotes was
T12791 2129-2137 VBN denotes selected
T12803 2137-2138 . denotes .
T12804 2138-2288 sentence denotes Isl1Cre and Hb9Cre mouse strains have been described [33,43] and Bax+/− animals were from Jackson Laboratory (Bar Harbor, Maine, United States) [27].
T12805 2139-2146 NN denotes Isl1Cre
T12807 2147-2150 CC denotes and
T12808 2151-2157 NN denotes Hb9Cre
T12809 2158-2163 NN denotes mouse
T12806 2164-2171 NNS denotes strains
T12811 2172-2176 VBP denotes have
T12812 2177-2181 VBN denotes been
T12810 2182-2191 VBN denotes described
T12813 2192-2193 -LRB- denotes [
T12815 2193-2195 CD denotes 33
T12816 2195-2196 , denotes ,
T12814 2196-2198 CD denotes 43
T12817 2198-2199 -RRB- denotes ]
T12818 2200-2203 CC denotes and
T12819 2204-2207 NN denotes Bax
T12821 2207-2208 SYM denotes +
T12822 2208-2209 HYPH denotes /
T12823 2209-2210 SYM denotes −
T12820 2211-2218 NNS denotes animals
T12824 2219-2223 VBD denotes were
T12825 2224-2228 IN denotes from
T12826 2229-2236 NNP denotes Jackson
T12827 2237-2247 NNP denotes Laboratory
T12828 2248-2249 -LRB- denotes (
T12830 2249-2252 NNP denotes Bar
T12829 2253-2259 NNP denotes Harbor
T12831 2259-2261 , denotes ,
T12832 2261-2266 NNP denotes Maine
T12833 2266-2268 , denotes ,
T12834 2268-2274 NNP denotes United
T12835 2275-2281 NNP denotes States
T12836 2281-2282 -RRB- denotes )
T12837 2283-2284 -LRB- denotes [
T12838 2284-2286 CD denotes 27
T12839 2286-2287 -RRB- denotes ]
T12840 2287-2288 . denotes .
T12841 2288-2423 sentence denotes Timed pregnancies were set up to generate embryos of different developmental stages with all genotypes described throughout the study.
T12842 2289-2294 JJ denotes Timed
T12843 2295-2306 NNS denotes pregnancies
T12845 2307-2311 VBD denotes were
T12844 2312-2315 VBN denotes set
T12846 2316-2318 RP denotes up
T12847 2319-2321 TO denotes to
T12848 2322-2330 VB denotes generate
T12849 2331-2338 NNS denotes embryos
T12850 2339-2341 IN denotes of
T12851 2342-2351 JJ denotes different
T12853 2352-2365 JJ denotes developmental
T12852 2366-2372 NNS denotes stages
T12854 2373-2377 IN denotes with
T12856 2378-2381 DT denotes all
T12857 2382-2391 NNS denotes genotypes
T12855 2392-2401 VBN denotes described
T12858 2402-2412 IN denotes throughout
T12859 2413-2416 DT denotes the
T12860 2417-2422 NN denotes study
T12861 2422-2423 . denotes .
T13099 2425-2440 JJ denotes Transcriptional
T13100 2441-2456 NN denotes transactivation
T13101 2457-2463 NNS denotes assays
T13102 2463-2660 sentence denotes The following plasmids were used for transcriptional transactivation assays: pRc/RSV (Invitrogen, Carlsbad, California, United States), pRc/RSV-Er81, pRc/RSV-EWS-Pea3, pTP-5xETS, and pTP-5xETSmut.
T13103 2464-2467 DT denotes The
T13105 2468-2477 VBG denotes following
T13104 2478-2486 NNS denotes plasmids
T13107 2487-2491 VBD denotes were
T13106 2492-2496 VBN denotes used
T13108 2497-2500 IN denotes for
T13109 2501-2516 JJ denotes transcriptional
T13111 2517-2532 NN denotes transactivation
T13110 2533-2539 NNS denotes assays
T13112 2539-2541 : denotes :
T13113 2541-2544 NN denotes pRc
T13115 2544-2545 HYPH denotes /
T13114 2545-2548 NN denotes RSV
T13116 2549-2550 -LRB- denotes (
T13117 2550-2560 NNP denotes Invitrogen
T13118 2560-2562 , denotes ,
T13119 2562-2570 NNP denotes Carlsbad
T13120 2570-2572 , denotes ,
T13121 2572-2582 NNP denotes California
T13122 2582-2584 , denotes ,
T13123 2584-2590 NNP denotes United
T13124 2591-2597 NNP denotes States
T13125 2597-2598 -RRB- denotes )
T13126 2598-2600 , denotes ,
T13127 2600-2603 NN denotes pRc
T13129 2603-2604 HYPH denotes /
T13128 2604-2607 NN denotes RSV
T13131 2607-2608 HYPH denotes -
T13130 2608-2612 NN denotes Er81
T13132 2612-2614 , denotes ,
T13133 2614-2617 NN denotes pRc
T13135 2617-2618 HYPH denotes /
T13134 2618-2621 NN denotes RSV
T13137 2621-2622 HYPH denotes -
T13138 2622-2625 NN denotes EWS
T13139 2625-2626 HYPH denotes -
T13136 2626-2630 NN denotes Pea3
T13140 2630-2632 , denotes ,
T13141 2632-2635 NN denotes pTP
T13143 2635-2636 HYPH denotes -
T13142 2636-2641 NN denotes 5xETS
T13144 2641-2643 , denotes ,
T13145 2643-2646 CC denotes and
T13146 2647-2650 NN denotes pTP
T13148 2650-2651 HYPH denotes -
T13147 2651-2659 NN denotes 5xETSmut
T13149 2659-2660 . denotes .
T13150 2660-2795 sentence denotes pRc/RSV-Er81 and pRc/RSV-EWS-Pea3 were obtained by insertion of the cDNAs for Er81 or EWS-Pea3 (gift from J. A. Hassell) into pRc/RSV.
T13151 2661-2664 NN denotes pRc
T13153 2664-2665 HYPH denotes /
T13152 2665-2668 NN denotes RSV
T13155 2668-2669 HYPH denotes -
T13154 2669-2673 NN denotes Er81
T13157 2674-2677 CC denotes and
T13158 2678-2681 NN denotes pRc
T13160 2681-2682 HYPH denotes /
T13159 2682-2685 NN denotes RSV
T13162 2685-2686 HYPH denotes -
T13163 2686-2689 NN denotes EWS
T13164 2689-2690 HYPH denotes -
T13161 2690-2694 NN denotes Pea3
T13165 2695-2699 VBD denotes were
T13156 2700-2708 VBN denotes obtained
T13166 2709-2711 IN denotes by
T13167 2712-2721 NN denotes insertion
T13168 2722-2724 IN denotes of
T13169 2725-2728 DT denotes the
T13170 2729-2734 NNS denotes cDNAs
T13171 2735-2738 IN denotes for
T13172 2739-2743 NN denotes Er81
T13173 2744-2746 CC denotes or
T13174 2747-2750 NN denotes EWS
T13176 2750-2751 HYPH denotes -
T13175 2751-2755 NN denotes Pea3
T13177 2756-2757 -LRB- denotes (
T13178 2757-2761 NN denotes gift
T13179 2762-2766 IN denotes from
T13180 2767-2769 NNP denotes J.
T13182 2770-2772 NNP denotes A.
T13181 2773-2780 NNP denotes Hassell
T13183 2780-2781 -RRB- denotes )
T13184 2782-2786 IN denotes into
T13185 2787-2790 NN denotes pRc
T13187 2790-2791 HYPH denotes /
T13186 2791-2794 NN denotes RSV
T13188 2794-2795 . denotes .
T13189 2795-2970 sentence denotes pTP-5xETS was constructed by inserting a cassette of five repetitive copies of high-affinity Pea3 binding sites (5′- GCCGGAAGC-3′) [18,19] into a modified version of pTK-Luc.
T13190 2796-2799 NN denotes pTP
T13192 2799-2800 HYPH denotes -
T13191 2800-2805 NN denotes 5xETS
T13194 2806-2809 VBD denotes was
T13193 2810-2821 VBN denotes constructed
T13195 2822-2824 IN denotes by
T13196 2825-2834 VBG denotes inserting
T13197 2835-2836 DT denotes a
T13198 2837-2845 NN denotes cassette
T13199 2846-2848 IN denotes of
T13200 2849-2853 CD denotes five
T13202 2854-2864 JJ denotes repetitive
T13201 2865-2871 NNS denotes copies
T13203 2872-2874 IN denotes of
T13204 2875-2879 JJ denotes high
T13206 2879-2880 HYPH denotes -
T13205 2880-2888 NN denotes affinity
T13208 2889-2893 NN denotes Pea3
T13209 2894-2901 NN denotes binding
T13207 2902-2907 NNS denotes sites
T13210 2908-2909 -LRB- denotes (
T13212 2909-2910 CD denotes 5
T13213 2910-2911 SYM denotes ′
T13214 2911-2912 HYPH denotes -
T13215 2913-2922 NN denotes GCCGGAAGC
T13216 2922-2923 HYPH denotes -
T13211 2923-2924 CD denotes 3
T13217 2924-2925 SYM denotes ′
T13218 2925-2926 -RRB- denotes )
T13219 2927-2928 -LRB- denotes [
T13221 2928-2930 CD denotes 18
T13222 2930-2931 , denotes ,
T13220 2931-2933 CD denotes 19
T13223 2933-2934 -RRB- denotes ]
T13224 2935-2939 IN denotes into
T13225 2940-2941 DT denotes a
T13227 2942-2950 VBN denotes modified
T13226 2951-2958 NN denotes version
T13228 2959-2961 IN denotes of
T13229 2962-2965 NN denotes pTK
T13231 2965-2966 HYPH denotes -
T13230 2966-2969 NN denotes Luc
T13232 2969-2970 . denotes .
T13233 2970-3087 sentence denotes pTP-5xETSmut was generated as pTP-5xETS but using a mutated complement of the Pea3 binding sites (5′- GCCTATGGC-3′).
T13234 2971-2974 NN denotes pTP
T13236 2974-2975 HYPH denotes -
T13235 2975-2983 NN denotes 5xETSmut
T13238 2984-2987 VBD denotes was
T13237 2988-2997 VBN denotes generated
T13239 2998-3000 IN denotes as
T13240 3001-3004 NN denotes pTP
T13242 3004-3005 HYPH denotes -
T13241 3005-3010 NN denotes 5xETS
T13243 3011-3014 CC denotes but
T13244 3015-3020 VBG denotes using
T13245 3021-3022 DT denotes a
T13247 3023-3030 VBN denotes mutated
T13246 3031-3041 NN denotes complement
T13248 3042-3044 IN denotes of
T13249 3045-3048 DT denotes the
T13251 3049-3053 NN denotes Pea3
T13252 3054-3061 NN denotes binding
T13250 3062-3067 NNS denotes sites
T13253 3068-3069 -LRB- denotes (
T13255 3069-3070 CD denotes 5
T13256 3070-3071 SYM denotes ′
T13257 3071-3072 HYPH denotes -
T13258 3073-3082 NN denotes GCCTATGGC
T13259 3082-3083 HYPH denotes -
T13254 3083-3084 CD denotes 3
T13260 3084-3085 SYM denotes ′
T13261 3085-3086 -RRB- denotes )
T13262 3086-3087 . denotes .
T13263 3087-3196 sentence denotes A control plasmid to normalize for transfection efficiency (placZ) and pTK-Luc were a gift from D. Kressler.
T13264 3088-3089 DT denotes A
T13266 3090-3097 NN denotes control
T13265 3098-3105 NN denotes plasmid
T13268 3106-3108 TO denotes to
T13269 3109-3118 VB denotes normalize
T13270 3119-3122 IN denotes for
T13271 3123-3135 NN denotes transfection
T13272 3136-3146 NN denotes efficiency
T13273 3147-3148 -LRB- denotes (
T13274 3148-3153 NN denotes placZ
T13275 3153-3154 -RRB- denotes )
T13276 3155-3158 CC denotes and
T13277 3159-3162 NN denotes pTK
T13279 3162-3163 HYPH denotes -
T13278 3163-3166 NN denotes Luc
T13267 3167-3171 VBD denotes were
T13280 3172-3173 DT denotes a
T13281 3174-3178 NN denotes gift
T13282 3179-3183 IN denotes from
T13283 3184-3186 NNP denotes D.
T13284 3187-3195 NNP denotes Kressler
T13285 3195-3196 . denotes .
T13286 3196-3411 sentence denotes COS-7 cells were co-transfected with 1–1.2 μg of total DNA including one of the effector plasmids pRc/RSV-empty, pRc/RSV-Er81, or pRc/RSV-EWS-Pea3; one of the reporter plasmids pTP-5xETS or pTP-5xETSmut; and placZ.
T13287 3197-3200 NN denotes COS
T13289 3200-3201 HYPH denotes -
T13290 3201-3202 CD denotes 7
T13288 3203-3208 NNS denotes cells
T13292 3209-3213 VBD denotes were
T13291 3214-3228 VBN denotes co-transfected
T13293 3229-3233 IN denotes with
T13294 3234-3235 CD denotes 1
T13296 3235-3236 SYM denotes –
T13295 3236-3239 CD denotes 1.2
T13297 3240-3242 NN denotes μg
T13298 3243-3245 IN denotes of
T13299 3246-3251 JJ denotes total
T13300 3252-3255 NN denotes DNA
T13301 3256-3265 VBG denotes including
T13302 3266-3269 CD denotes one
T13303 3270-3272 IN denotes of
T13304 3273-3276 DT denotes the
T13306 3277-3285 NN denotes effector
T13305 3286-3294 NNS denotes plasmids
T13307 3295-3298 NN denotes pRc
T13309 3298-3299 HYPH denotes /
T13308 3299-3302 NN denotes RSV
T13310 3302-3303 HYPH denotes -
T13311 3303-3308 JJ denotes empty
T13312 3308-3310 , denotes ,
T13313 3310-3313 NN denotes pRc
T13315 3313-3314 HYPH denotes /
T13314 3314-3317 NN denotes RSV
T13317 3317-3318 HYPH denotes -
T13316 3318-3322 NN denotes Er81
T13318 3322-3324 , denotes ,
T13319 3324-3326 CC denotes or
T13320 3327-3330 NN denotes pRc
T13322 3330-3331 HYPH denotes /
T13321 3331-3334 NN denotes RSV
T13324 3334-3335 HYPH denotes -
T13325 3335-3338 NN denotes EWS
T13326 3338-3339 HYPH denotes -
T13323 3339-3343 NN denotes Pea3
T13327 3343-3344 : denotes ;
T13328 3345-3348 CD denotes one
T13329 3349-3351 IN denotes of
T13330 3352-3355 DT denotes the
T13332 3356-3364 NN denotes reporter
T13331 3365-3373 NNS denotes plasmids
T13333 3374-3377 NN denotes pTP
T13335 3377-3378 HYPH denotes -
T13334 3378-3383 NN denotes 5xETS
T13336 3384-3386 CC denotes or
T13337 3387-3390 NN denotes pTP
T13339 3390-3391 HYPH denotes -
T13338 3391-3399 NN denotes 5xETSmut
T13340 3399-3400 : denotes ;
T13341 3401-3404 CC denotes and
T13342 3405-3410 NN denotes placZ
T13343 3410-3411 . denotes .
T13344 3411-3540 sentence denotes Cells were harvested after 25 h and processed for assays to determine luciferase and LacZ activity as described previously [44].
T13345 3412-3417 NNS denotes Cells
T13347 3418-3422 VBD denotes were
T13346 3423-3432 VBN denotes harvested
T13348 3433-3438 IN denotes after
T13349 3439-3441 CD denotes 25
T13350 3442-3443 NN denotes h
T13351 3444-3447 CC denotes and
T13352 3448-3457 VBN denotes processed
T13353 3458-3461 IN denotes for
T13354 3462-3468 NNS denotes assays
T13355 3469-3471 TO denotes to
T13356 3472-3481 VB denotes determine
T13357 3482-3492 NN denotes luciferase
T13359 3493-3496 CC denotes and
T13360 3497-3501 NN denotes LacZ
T13358 3502-3510 NN denotes activity
T13361 3511-3513 IN denotes as
T13362 3514-3523 VBN denotes described
T13363 3524-3534 RB denotes previously
T13364 3535-3536 -LRB- denotes [
T13365 3536-3538 CD denotes 44
T13366 3538-3539 -RRB- denotes ]
T13367 3539-3540 . denotes .
T13368 3540-3623 sentence denotes Luciferase values normalized to LacZ activity are referred to as luciferase units.
T13369 3541-3551 NN denotes Luciferase
T13370 3552-3558 NNS denotes values
T13372 3559-3569 VBN denotes normalized
T13373 3570-3572 IN denotes to
T13374 3573-3577 NN denotes LacZ
T13375 3578-3586 NN denotes activity
T13376 3587-3590 VBP denotes are
T13371 3591-3599 VBN denotes referred
T13377 3600-3602 IN denotes to
T13378 3603-3605 IN denotes as
T13379 3606-3616 NN denotes luciferase
T13380 3617-3622 NNS denotes units
T13381 3622-3623 . denotes .
T13917 3625-3627 FW denotes In
T13918 3628-3632 FW denotes situ
T13919 3633-3646 NN denotes hybridization
T13920 3647-3650 CC denotes and
T13921 3651-3671 NN denotes immunohistochemistry
T13922 3671-3854 sentence denotes For in situ hybridization analysis, cryostat sections were hybridized using digoxigenin-labeled probes [45] directed against mouse TrkA or TrkB, or rat TrkC (gift from L. F. Parada).
T13923 3672-3675 IN denotes For
T13925 3676-3678 FW denotes in
T13926 3679-3683 FW denotes situ
T13928 3684-3697 NN denotes hybridization
T13927 3698-3706 NN denotes analysis
T13929 3706-3708 , denotes ,
T13930 3708-3716 NN denotes cryostat
T13931 3717-3725 NNS denotes sections
T13932 3726-3730 VBD denotes were
T13924 3731-3741 VBN denotes hybridized
T13933 3742-3747 VBG denotes using
T13934 3748-3759 NN denotes digoxigenin
T13936 3759-3760 HYPH denotes -
T13935 3760-3767 VBN denotes labeled
T13937 3768-3774 NNS denotes probes
T13938 3775-3776 -LRB- denotes [
T13939 3776-3778 CD denotes 45
T13940 3778-3779 -RRB- denotes ]
T13941 3780-3788 VBN denotes directed
T13942 3789-3796 IN denotes against
T13943 3797-3802 NN denotes mouse
T13944 3803-3807 NN denotes TrkA
T13945 3808-3810 CC denotes or
T13946 3811-3815 NN denotes TrkB
T13947 3815-3817 , denotes ,
T13948 3817-3819 CC denotes or
T13949 3820-3823 NN denotes rat
T13950 3824-3828 NN denotes TrkC
T13951 3829-3830 -LRB- denotes (
T13952 3830-3834 NN denotes gift
T13953 3835-3839 IN denotes from
T13954 3840-3842 NNP denotes L.
T13956 3843-3845 NNP denotes F.
T13955 3846-3852 NNP denotes Parada
T13957 3852-3853 -RRB- denotes )
T13958 3853-3854 . denotes .
T13959 3854-4684 sentence denotes Antibodies used in this study were as follows: rabbit anti-Er81 [14], rabbit anti-Pea3 [14], rabbit anti-PV [14], rabbit anti-eGFP (Molecular Probes, Eugene, Oregon, United States), rabbit anti-Calbindin, rabbit anti-Calretinin (Swant, Bellinzona, Switzerland), rabbit anti-CGRP (Chemicon, Temecula, California, United States), rabbit anti-vGlut1 (Synaptic Systems, Goettingen, Germany), rabbit anti-Brn3a (gift from E. Turner), rabbit anti-TrkA and -p75 (gift from L. F. Reichardt), rabbit anti-Runx3 (Kramer and Arber, unpublished reagent), rabbit anti-Rhodamine (Molecular Probes), mouse anti-neurofilament (American Type Culture Collection, Manassas, Virginia, United States), sheep anti-eGFP (Biogenesis, Poole, United Kingdom), goat anti-LacZ [14], goat anti-TrkC (gift from L. F. Reichardt), and guinea pig anti-Isl1 [14].
T13960 3855-3865 NNS denotes Antibodies
T13962 3866-3870 VBN denotes used
T13963 3871-3873 IN denotes in
T13964 3874-3878 DT denotes this
T13965 3879-3884 NN denotes study
T13961 3885-3889 VBD denotes were
T13966 3890-3892 IN denotes as
T13967 3893-3900 VBZ denotes follows
T13968 3900-3902 : denotes :
T13969 3902-3908 NN denotes rabbit
T13970 3909-3918 NN denotes anti-Er81
T13971 3919-3920 -LRB- denotes [
T13972 3920-3922 CD denotes 14
T13973 3922-3923 -RRB- denotes ]
T13974 3923-3925 , denotes ,
T13975 3925-3931 NN denotes rabbit
T13976 3932-3941 JJ denotes anti-Pea3
T13977 3942-3943 -LRB- denotes [
T13978 3943-3945 CD denotes 14
T13979 3945-3946 -RRB- denotes ]
T13980 3946-3948 , denotes ,
T13981 3948-3954 NN denotes rabbit
T13982 3955-3962 JJ denotes anti-PV
T13983 3963-3964 -LRB- denotes [
T13984 3964-3966 CD denotes 14
T13985 3966-3967 -RRB- denotes ]
T13986 3967-3969 , denotes ,
T13987 3969-3975 NN denotes rabbit
T13988 3976-3985 JJ denotes anti-eGFP
T13989 3986-3987 -LRB- denotes (
T13991 3987-3996 NNP denotes Molecular
T13990 3997-4003 NNP denotes Probes
T13992 4003-4005 , denotes ,
T13993 4005-4011 NNP denotes Eugene
T13994 4011-4013 , denotes ,
T13995 4013-4019 NNP denotes Oregon
T13996 4019-4021 , denotes ,
T13997 4021-4027 NNP denotes United
T13998 4028-4034 NNP denotes States
T13999 4034-4035 -RRB- denotes )
T14000 4035-4037 , denotes ,
T14001 4037-4043 NN denotes rabbit
T14002 4044-4058 JJ denotes anti-Calbindin
T14003 4058-4060 , denotes ,
T14004 4060-4066 NN denotes rabbit
T14005 4067-4082 JJ denotes anti-Calretinin
T14006 4083-4084 -LRB- denotes (
T14007 4084-4089 NNP denotes Swant
T14008 4089-4091 , denotes ,
T14009 4091-4101 NNP denotes Bellinzona
T14010 4101-4103 , denotes ,
T14011 4103-4114 NNP denotes Switzerland
T14012 4114-4115 -RRB- denotes )
T14013 4115-4117 , denotes ,
T14014 4117-4123 NN denotes rabbit
T14015 4124-4133 JJ denotes anti-CGRP
T14016 4134-4135 -LRB- denotes (
T14017 4135-4143 NNP denotes Chemicon
T14018 4143-4145 , denotes ,
T14019 4145-4153 NNP denotes Temecula
T14020 4153-4155 , denotes ,
T14021 4155-4165 NNP denotes California
T14022 4165-4167 , denotes ,
T14023 4167-4173 NNP denotes United
T14024 4174-4180 NNP denotes States
T14025 4180-4181 -RRB- denotes )
T14026 4181-4183 , denotes ,
T14027 4183-4189 NN denotes rabbit
T14028 4190-4201 JJ denotes anti-vGlut1
T14029 4202-4203 -LRB- denotes (
T14031 4203-4211 NN denotes Synaptic
T14030 4212-4219 NNP denotes Systems
T14032 4219-4221 , denotes ,
T14033 4221-4231 NNP denotes Goettingen
T14034 4231-4233 , denotes ,
T14035 4233-4240 NNP denotes Germany
T14036 4240-4241 -RRB- denotes )
T14037 4241-4243 , denotes ,
T14038 4243-4249 NN denotes rabbit
T14039 4250-4260 JJ denotes anti-Brn3a
T14040 4261-4262 -LRB- denotes (
T14041 4262-4266 NN denotes gift
T14042 4267-4271 IN denotes from
T14043 4272-4274 NNP denotes E.
T14044 4275-4281 NNP denotes Turner
T14045 4281-4282 -RRB- denotes )
T14046 4282-4284 , denotes ,
T14047 4284-4290 NN denotes rabbit
T14049 4291-4300 JJ denotes anti-TrkA
T14050 4301-4304 CC denotes and
T14051 4305-4306 HYPH denotes -
T14048 4306-4309 NN denotes p75
T14052 4310-4311 -LRB- denotes (
T14053 4311-4315 NN denotes gift
T14054 4316-4320 IN denotes from
T14055 4321-4323 NNP denotes L.
T14057 4324-4326 NNP denotes F.
T14056 4327-4336 NNP denotes Reichardt
T14058 4336-4337 -RRB- denotes )
T14059 4337-4339 , denotes ,
T14060 4339-4345 NN denotes rabbit
T14061 4346-4356 JJ denotes anti-Runx3
T14062 4357-4358 -LRB- denotes (
T14063 4358-4364 NNP denotes Kramer
T14064 4365-4368 CC denotes and
T14065 4369-4374 NNP denotes Arber
T14066 4374-4376 , denotes ,
T14067 4376-4387 JJ denotes unpublished
T14068 4388-4395 NN denotes reagent
T14069 4395-4396 -RRB- denotes )
T14070 4396-4398 , denotes ,
T14071 4398-4404 NN denotes rabbit
T14072 4405-4419 JJ denotes anti-Rhodamine
T14073 4420-4421 -LRB- denotes (
T14075 4421-4430 NNP denotes Molecular
T14074 4431-4437 NNP denotes Probes
T14076 4437-4438 -RRB- denotes )
T14077 4438-4440 , denotes ,
T14078 4440-4445 NN denotes mouse
T14079 4446-4464 JJ denotes anti-neurofilament
T14080 4465-4466 -LRB- denotes (
T14082 4466-4474 NNP denotes American
T14083 4475-4479 NNP denotes Type
T14084 4480-4487 NNP denotes Culture
T14081 4488-4498 NNP denotes Collection
T14085 4498-4500 , denotes ,
T14086 4500-4508 NNP denotes Manassas
T14087 4508-4510 , denotes ,
T14088 4510-4518 NNP denotes Virginia
T14089 4518-4520 , denotes ,
T14090 4520-4526 NNP denotes United
T14091 4527-4533 NNP denotes States
T14092 4533-4534 -RRB- denotes )
T14093 4534-4536 , denotes ,
T14094 4536-4541 NN denotes sheep
T14095 4542-4551 JJ denotes anti-eGFP
T14096 4552-4553 -LRB- denotes (
T14097 4553-4563 NNP denotes Biogenesis
T14098 4563-4565 , denotes ,
T14099 4565-4570 NNP denotes Poole
T14100 4570-4572 , denotes ,
T14101 4572-4578 NNP denotes United
T14102 4579-4586 NNP denotes Kingdom
T14103 4586-4587 -RRB- denotes )
T14104 4587-4589 , denotes ,
T14105 4589-4593 NN denotes goat
T14106 4594-4603 JJ denotes anti-LacZ
T14107 4604-4605 -LRB- denotes [
T14108 4605-4607 CD denotes 14
T14109 4607-4608 -RRB- denotes ]
T14110 4608-4610 , denotes ,
T14111 4610-4614 NN denotes goat
T14112 4615-4624 JJ denotes anti-TrkC
T14113 4625-4626 -LRB- denotes (
T14114 4626-4630 NN denotes gift
T14115 4631-4635 IN denotes from
T14116 4636-4638 NNP denotes L.
T14118 4639-4641 NNP denotes F.
T14117 4642-4651 NNP denotes Reichardt
T14119 4651-4652 -RRB- denotes )
T14120 4652-4654 , denotes ,
T14121 4654-4657 CC denotes and
T14122 4658-4664 NN denotes guinea
T14123 4665-4668 NN denotes pig
T14124 4669-4678 JJ denotes anti-Isl1
T14125 4679-4680 -LRB- denotes [
T14126 4680-4682 CD denotes 14
T14127 4682-4683 -RRB- denotes ]
T14128 4683-4684 . denotes .
T14129 4684-4910 sentence denotes Terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL) to detect apoptotic cells in E13.5 DRG on cryostat sections was performed as described by the manufacturer (Roche, Basel, Switzerland).
T14130 4685-4693 JJ denotes Terminal
T14132 4694-4710 NN denotes deoxynucleotidyl
T14131 4711-4722 NN denotes transferase
T14134 4722-4723 HYPH denotes -
T14133 4723-4731 VBN denotes mediated
T14136 4732-4744 VBN denotes biotinylated
T14137 4745-4748 NN denotes UTP
T14138 4749-4753 NN denotes nick
T14139 4754-4757 NN denotes end
T14135 4758-4766 NN denotes labeling
T14141 4767-4768 -LRB- denotes (
T14142 4768-4773 NN denotes TUNEL
T14143 4773-4774 -RRB- denotes )
T14144 4775-4777 TO denotes to
T14145 4778-4784 VB denotes detect
T14146 4785-4794 JJ denotes apoptotic
T14147 4795-4800 NNS denotes cells
T14148 4801-4803 IN denotes in
T14149 4804-4809 NN denotes E13.5
T14150 4810-4813 NN denotes DRG
T14151 4814-4816 IN denotes on
T14152 4817-4825 NN denotes cryostat
T14153 4826-4834 NNS denotes sections
T14154 4835-4838 VBD denotes was
T14140 4839-4848 VBN denotes performed
T14155 4849-4851 IN denotes as
T14156 4852-4861 VBN denotes described
T14157 4862-4864 IN denotes by
T14158 4865-4868 DT denotes the
T14159 4869-4881 NN denotes manufacturer
T14160 4882-4883 -LRB- denotes (
T14161 4883-4888 NNP denotes Roche
T14162 4888-4890 , denotes ,
T14163 4890-4895 NNP denotes Basel
T14164 4895-4897 , denotes ,
T14165 4897-4908 NNP denotes Switzerland
T14166 4908-4909 -RRB- denotes )
T14167 4909-4910 . denotes .
T14168 4910-4997 sentence denotes Quantitative analysis of TUNEL+ DRG cells was performed essentially as described [27].
T14169 4911-4923 JJ denotes Quantitative
T14170 4924-4932 NN denotes analysis
T14172 4933-4935 IN denotes of
T14173 4936-4941 NN denotes TUNEL
T14175 4941-4942 SYM denotes +
T14176 4943-4946 NN denotes DRG
T14174 4947-4952 NNS denotes cells
T14177 4953-4956 VBD denotes was
T14171 4957-4966 VBN denotes performed
T14178 4967-4978 RB denotes essentially
T14180 4979-4981 IN denotes as
T14179 4982-4991 VBN denotes described
T14181 4992-4993 -LRB- denotes [
T14182 4993-4995 CD denotes 27
T14183 4995-4996 -RRB- denotes ]
T14184 4996-4997 . denotes .
T14185 4997-5101 sentence denotes BrdU pulse-chase experiments and LacZ wholemount stainings were performed as previously described [46].
T14186 4998-5002 NN denotes BrdU
T14188 5003-5008 NN denotes pulse
T14190 5008-5009 HYPH denotes -
T14189 5009-5014 NN denotes chase
T14187 5015-5026 NNS denotes experiments
T14192 5027-5030 CC denotes and
T14193 5031-5035 NN denotes LacZ
T14195 5036-5046 NN denotes wholemount
T14194 5047-5056 NNS denotes stainings
T14196 5057-5061 VBD denotes were
T14191 5062-5071 VBN denotes performed
T14197 5072-5074 IN denotes as
T14199 5075-5085 RB denotes previously
T14198 5086-5095 VBN denotes described
T14200 5096-5097 -LRB- denotes [
T14201 5097-5099 CD denotes 46
T14202 5099-5100 -RRB- denotes ]
T14203 5100-5101 . denotes .
T14204 5101-5373 sentence denotes For anterograde tracing experiments to visualize projections of sensory neurons, rhodamine-conjugated dextran (Molecular Probes) was injected into single lumbar (L3) DRG at E13.5 or applied to whole lumbar dorsal roots (L3) at postnatal day (P) 5 using glass capillaries.
T14205 5102-5105 IN denotes For
T14207 5106-5117 JJ denotes anterograde
T14209 5118-5125 NN denotes tracing
T14208 5126-5137 NNS denotes experiments
T14210 5138-5140 TO denotes to
T14206 5141-5150 VB denotes visualize
T14212 5151-5162 NNS denotes projections
T14213 5163-5165 IN denotes of
T14214 5166-5173 JJ denotes sensory
T14215 5174-5181 NNS denotes neurons
T14216 5181-5183 , denotes ,
T14217 5183-5192 NN denotes rhodamine
T14219 5192-5193 HYPH denotes -
T14218 5193-5203 VBN denotes conjugated
T14220 5204-5211 NN denotes dextran
T14221 5212-5213 -LRB- denotes (
T14223 5213-5222 NNP denotes Molecular
T14222 5223-5229 NNP denotes Probes
T14224 5229-5230 -RRB- denotes )
T14225 5231-5234 VBD denotes was
T14211 5235-5243 VBN denotes injected
T14226 5244-5248 IN denotes into
T14227 5249-5255 JJ denotes single
T14229 5256-5262 NN denotes lumbar
T14230 5263-5264 -LRB- denotes (
T14231 5264-5266 NN denotes L3
T14232 5266-5267 -RRB- denotes )
T14228 5268-5271 NN denotes DRG
T14233 5272-5274 IN denotes at
T14234 5275-5280 NN denotes E13.5
T14235 5281-5283 CC denotes or
T14236 5284-5291 VBN denotes applied
T14237 5292-5294 IN denotes to
T14238 5295-5300 JJ denotes whole
T14240 5301-5307 JJ denotes lumbar
T14241 5308-5314 JJ denotes dorsal
T14239 5315-5320 NNS denotes roots
T14242 5321-5322 -LRB- denotes (
T14243 5322-5324 NN denotes L3
T14244 5324-5325 -RRB- denotes )
T14245 5326-5328 IN denotes at
T14246 5329-5338 JJ denotes postnatal
T14247 5339-5342 NN denotes day
T14248 5343-5344 -LRB- denotes (
T14249 5344-5345 NN denotes P
T14250 5345-5346 -RRB- denotes )
T14251 5347-5348 CD denotes 5
T14252 5349-5354 VBG denotes using
T14253 5355-5360 NN denotes glass
T14254 5361-5372 NNS denotes capillaries
T14255 5372-5373 . denotes .
T14256 5373-5450 sentence denotes After injection, animals were incubated for 2–3 h (E13.5) or overnight (P5).
T14257 5374-5379 IN denotes After
T14259 5380-5389 NN denotes injection
T14260 5389-5391 , denotes ,
T14261 5391-5398 NNS denotes animals
T14262 5399-5403 VBD denotes were
T14258 5404-5413 VBN denotes incubated
T14263 5414-5417 IN denotes for
T14264 5418-5419 CD denotes 2
T14266 5419-5420 SYM denotes –
T14265 5420-5421 CD denotes 3
T14267 5422-5423 NN denotes h
T14268 5424-5425 -LRB- denotes (
T14269 5425-5430 NN denotes E13.5
T14270 5430-5431 -RRB- denotes )
T14271 5432-5434 CC denotes or
T14272 5435-5444 RB denotes overnight
T14273 5445-5446 -LRB- denotes (
T14274 5446-5448 NN denotes P5
T14275 5448-5449 -RRB- denotes )
T14276 5449-5450 . denotes .
T14277 5450-5605 sentence denotes Cryostat sections were processed for immunohistochemistry as described [14] using fluorophore-conjugated secondary antibodies (1:1,000, Molecular Probes).
T14278 5451-5459 NN denotes Cryostat
T14279 5460-5468 NNS denotes sections
T14281 5469-5473 VBD denotes were
T14280 5474-5483 VBN denotes processed
T14282 5484-5487 IN denotes for
T14283 5488-5508 NN denotes immunohistochemistry
T14284 5509-5511 IN denotes as
T14285 5512-5521 VBN denotes described
T14286 5522-5523 -LRB- denotes [
T14287 5523-5525 CD denotes 14
T14288 5525-5526 -RRB- denotes ]
T14289 5527-5532 VBG denotes using
T14290 5533-5544 NN denotes fluorophore
T14292 5544-5545 HYPH denotes -
T14291 5545-5555 VBN denotes conjugated
T14294 5556-5565 JJ denotes secondary
T14293 5566-5576 NNS denotes antibodies
T14295 5577-5578 -LRB- denotes (
T14297 5578-5579 CD denotes 1
T14298 5579-5580 SYM denotes :
T14299 5580-5585 CD denotes 1,000
T14300 5585-5587 , denotes ,
T14301 5587-5596 NNP denotes Molecular
T14296 5597-5603 NNP denotes Probes
T14302 5603-5604 -RRB- denotes )
T14303 5604-5605 . denotes .
T14304 5605-5677 sentence denotes Images were collected on an Olympus (Tokyo, Japan) confocal microscope.
T14305 5606-5612 NNS denotes Images
T14307 5613-5617 VBD denotes were
T14306 5618-5627 VBN denotes collected
T14308 5628-5630 IN denotes on
T14309 5631-5633 DT denotes an
T14311 5634-5641 NNP denotes Olympus
T14312 5642-5643 -LRB- denotes (
T14313 5643-5648 NNP denotes Tokyo
T14314 5648-5650 , denotes ,
T14315 5650-5655 NNP denotes Japan
T14316 5655-5656 -RRB- denotes )
T14317 5657-5665 JJ denotes confocal
T14310 5666-5676 NN denotes microscope
T14318 5676-5677 . denotes .
T14319 5677-5941 sentence denotes Images from in situ hybridization experiments were collected with an RT-SPOT camera (Diagnostic Instruments, Sterling Heights, Michigan, United States), and Corel (Eden Prairie, Minnesota, United States) Photo Paint 10.0 was used for digital processing of images.
T14320 5678-5684 NNS denotes Images
T14322 5685-5689 IN denotes from
T14323 5690-5692 FW denotes in
T14324 5693-5697 FW denotes situ
T14326 5698-5711 NN denotes hybridization
T14325 5712-5723 NNS denotes experiments
T14327 5724-5728 VBD denotes were
T14321 5729-5738 VBN denotes collected
T14328 5739-5743 IN denotes with
T14329 5744-5746 DT denotes an
T14331 5747-5749 NN denotes RT
T14333 5749-5750 HYPH denotes -
T14332 5750-5754 NN denotes SPOT
T14330 5755-5761 NN denotes camera
T14334 5762-5763 -LRB- denotes (
T14336 5763-5773 JJ denotes Diagnostic
T14335 5774-5785 NNPS denotes Instruments
T14337 5785-5787 , denotes ,
T14338 5787-5795 NNP denotes Sterling
T14339 5796-5803 NNP denotes Heights
T14340 5803-5805 , denotes ,
T14341 5805-5813 NNP denotes Michigan
T14342 5813-5815 , denotes ,
T14343 5815-5821 NNP denotes United
T14344 5822-5828 NNP denotes States
T14345 5828-5829 -RRB- denotes )
T14346 5829-5831 , denotes ,
T14347 5831-5834 CC denotes and
T14348 5835-5840 NNP denotes Corel
T14350 5841-5842 -LRB- denotes (
T14352 5842-5846 NNP denotes Eden
T14351 5847-5854 NNP denotes Prairie
T14353 5854-5856 , denotes ,
T14354 5856-5865 NNP denotes Minnesota
T14355 5865-5867 , denotes ,
T14356 5867-5873 NNP denotes United
T14357 5874-5880 NNP denotes States
T14358 5880-5881 -RRB- denotes )
T14359 5882-5887 NNP denotes Photo
T14349 5888-5893 NNP denotes Paint
T14361 5894-5898 CD denotes 10.0
T14362 5899-5902 VBD denotes was
T14360 5903-5907 VBN denotes used
T14363 5908-5911 IN denotes for
T14364 5912-5919 JJ denotes digital
T14365 5920-5930 NN denotes processing
T14366 5931-5933 IN denotes of
T14367 5934-5940 NNS denotes images
T14368 5940-5941 . denotes .
T14469 5943-5945 FW denotes In
T14470 5946-5951 FW denotes vitro
T14471 5952-5960 NNS denotes cultures
T14472 5961-5963 IN denotes of
T14473 5964-5967 NN denotes DRG
T14474 5967-6379 sentence denotes Individual lumbar DRG were dissected from E13.5 or E14.5 embryos and placed on Matrigel (BD Biosciences, San Jose, California, United States) coated coverslips in DMEM/F12 (Gibco, San Diego, California, United States), 2 mM L-Gln (Gibco), N2 (Gibco), and 1 mg/ml BSA (Sigma, St. Louis, Missouri, United States) without neurotrophins, or supplemented with either NGF (100 ng/ml, Gibco) or NT-3 (20 ng/ml, Sigma).
T14475 5968-5978 JJ denotes Individual
T14477 5979-5985 NN denotes lumbar
T14476 5986-5989 NN denotes DRG
T14479 5990-5994 VBD denotes were
T14478 5995-6004 VBN denotes dissected
T14480 6005-6009 IN denotes from
T14481 6010-6015 NN denotes E13.5
T14483 6016-6018 CC denotes or
T14484 6019-6024 NN denotes E14.5
T14482 6025-6032 NNS denotes embryos
T14485 6033-6036 CC denotes and
T14486 6037-6043 VBN denotes placed
T14487 6044-6046 IN denotes on
T14488 6047-6055 NNP denotes Matrigel
T14490 6056-6057 -LRB- denotes (
T14492 6057-6059 NNP denotes BD
T14491 6060-6071 NNP denotes Biosciences
T14493 6071-6073 , denotes ,
T14494 6073-6076 NNP denotes San
T14495 6077-6081 NNP denotes Jose
T14496 6081-6083 , denotes ,
T14497 6083-6093 NNP denotes California
T14498 6093-6095 , denotes ,
T14499 6095-6101 NNP denotes United
T14500 6102-6108 NNP denotes States
T14501 6108-6109 -RRB- denotes )
T14489 6110-6116 VBN denotes coated
T14502 6117-6127 NNS denotes coverslips
T14503 6128-6130 IN denotes in
T14504 6131-6135 NN denotes DMEM
T14506 6135-6136 HYPH denotes /
T14505 6136-6139 NN denotes F12
T14507 6140-6141 -LRB- denotes (
T14508 6141-6146 NNP denotes Gibco
T14509 6146-6148 , denotes ,
T14510 6148-6151 NNP denotes San
T14511 6152-6157 NNP denotes Diego
T14512 6157-6159 , denotes ,
T14513 6159-6169 NNP denotes California
T14514 6169-6171 , denotes ,
T14515 6171-6177 NNP denotes United
T14516 6178-6184 NNP denotes States
T14517 6184-6185 -RRB- denotes )
T14518 6185-6187 , denotes ,
T14519 6187-6188 CD denotes 2
T14520 6189-6191 NN denotes mM
T14522 6192-6193 NN denotes L
T14523 6193-6194 HYPH denotes -
T14521 6194-6197 NN denotes Gln
T14524 6198-6199 -LRB- denotes (
T14525 6199-6204 NNP denotes Gibco
T14526 6204-6205 -RRB- denotes )
T14527 6205-6207 , denotes ,
T14528 6207-6209 NN denotes N2
T14529 6210-6211 -LRB- denotes (
T14530 6211-6216 NNP denotes Gibco
T14531 6216-6217 -RRB- denotes )
T14532 6217-6219 , denotes ,
T14533 6219-6222 CC denotes and
T14534 6223-6224 CD denotes 1
T14535 6225-6227 NN denotes mg
T14537 6227-6228 SYM denotes /
T14538 6228-6230 NN denotes ml
T14536 6231-6234 NN denotes BSA
T14539 6235-6236 -LRB- denotes (
T14540 6236-6241 NNP denotes Sigma
T14541 6241-6243 , denotes ,
T14542 6243-6246 NNP denotes St.
T14543 6247-6252 NNP denotes Louis
T14544 6252-6254 , denotes ,
T14545 6254-6262 NNP denotes Missouri
T14546 6262-6264 , denotes ,
T14547 6264-6270 NNP denotes United
T14548 6271-6277 NNP denotes States
T14549 6277-6278 -RRB- denotes )
T14550 6279-6286 IN denotes without
T14551 6287-6300 NNS denotes neurotrophins
T14552 6300-6302 , denotes ,
T14553 6302-6304 CC denotes or
T14554 6305-6317 VBN denotes supplemented
T14555 6318-6322 IN denotes with
T14556 6323-6329 CC denotes either
T14557 6330-6333 NN denotes NGF
T14558 6334-6335 -LRB- denotes (
T14560 6335-6338 CD denotes 100
T14561 6339-6341 NN denotes ng
T14562 6341-6342 SYM denotes /
T14563 6342-6344 NN denotes ml
T14564 6344-6346 , denotes ,
T14559 6346-6351 NNP denotes Gibco
T14565 6351-6352 -RRB- denotes )
T14566 6353-6355 CC denotes or
T14567 6356-6358 NN denotes NT
T14568 6358-6359 HYPH denotes -
T14569 6359-6360 CD denotes 3
T14570 6361-6362 -LRB- denotes (
T14572 6362-6364 CD denotes 20
T14573 6365-6367 NN denotes ng
T14574 6367-6368 SYM denotes /
T14575 6368-6370 NN denotes ml
T14576 6370-6372 , denotes ,
T14571 6372-6377 NNP denotes Sigma
T14577 6377-6378 -RRB- denotes )
T14578 6378-6379 . denotes .
T14579 6379-6519 sentence denotes DRG explants (n ≥ 20 for each condition) were cultured for 48 h, processed for immunocytochemistry, and analyzed using confocal microscopy.
T14580 6380-6383 NN denotes DRG
T14581 6384-6392 NNS denotes explants
T14583 6393-6394 -LRB- denotes (
T14585 6394-6395 NN denotes n
T14586 6396-6397 SYM denotes ≥
T14584 6398-6400 CD denotes 20
T14587 6401-6404 IN denotes for
T14588 6405-6409 DT denotes each
T14589 6410-6419 NN denotes condition
T14590 6419-6420 -RRB- denotes )
T14591 6421-6425 VBD denotes were
T14582 6426-6434 VBN denotes cultured
T14592 6435-6438 IN denotes for
T14593 6439-6441 CD denotes 48
T14594 6442-6443 NN denotes h
T14595 6443-6445 , denotes ,
T14596 6445-6454 VBN denotes processed
T14597 6455-6458 IN denotes for
T14598 6459-6478 NN denotes immunocytochemistry
T14599 6478-6480 , denotes ,
T14600 6480-6483 CC denotes and
T14601 6484-6492 VBN denotes analyzed
T14602 6493-6498 VBG denotes using
T14603 6499-6507 JJ denotes confocal
T14604 6508-6518 NN denotes microscopy
T14605 6518-6519 . denotes .
T14754 6521-6528 NNP denotes Western
T14755 6529-6533 NN denotes blot
T14756 6534-6542 NN denotes analysis
T14757 6542-6758 sentence denotes Lumbar DRG from E16.5 embryos were isolated, mechanically disrupted, homogenized using glass beads (Sigma), and lysed in standard lysis buffer supplemented with protease and phosphatase inhibitors as described [47].
T14758 6543-6549 NN denotes Lumbar
T14759 6550-6553 NN denotes DRG
T14761 6554-6558 IN denotes from
T14762 6559-6564 NN denotes E16.5
T14763 6565-6572 NNS denotes embryos
T14764 6573-6577 VBD denotes were
T14760 6578-6586 VBN denotes isolated
T14765 6586-6588 , denotes ,
T14766 6588-6600 RB denotes mechanically
T14767 6601-6610 VBN denotes disrupted
T14768 6610-6612 , denotes ,
T14769 6612-6623 VBN denotes homogenized
T14770 6624-6629 VBG denotes using
T14771 6630-6635 NN denotes glass
T14772 6636-6641 NNS denotes beads
T14773 6642-6643 -LRB- denotes (
T14774 6643-6648 NNP denotes Sigma
T14775 6648-6649 -RRB- denotes )
T14776 6649-6651 , denotes ,
T14777 6651-6654 CC denotes and
T14778 6655-6660 VBN denotes lysed
T14779 6661-6663 IN denotes in
T14780 6664-6672 JJ denotes standard
T14782 6673-6678 NN denotes lysis
T14781 6679-6685 NN denotes buffer
T14783 6686-6698 VBN denotes supplemented
T14784 6699-6703 IN denotes with
T14785 6704-6712 NN denotes protease
T14787 6713-6716 CC denotes and
T14788 6717-6728 NN denotes phosphatase
T14786 6729-6739 NNS denotes inhibitors
T14789 6740-6742 IN denotes as
T14790 6743-6752 VBN denotes described
T14791 6753-6754 -LRB- denotes [
T14792 6754-6756 CD denotes 47
T14793 6756-6757 -RRB- denotes ]
T14794 6757-6758 . denotes .
T14795 6758-7048 sentence denotes Protein extracts were resolved by SDS-PAGE, and immunoblotting was performed using antibodies against Akt, p-Akt (Ser473), CREB, p-CREB (Ser133), Bax, Bcl-xl (Cell Signaling Technology, Beverly, Massachusetts, United States), and Bcl2 (BD Pharmingen, San Diego, California, United States).
T14796 6759-6766 NN denotes Protein
T14797 6767-6775 NNS denotes extracts
T14799 6776-6780 VBD denotes were
T14798 6781-6789 VBN denotes resolved
T14800 6790-6792 IN denotes by
T14801 6793-6796 NN denotes SDS
T14803 6796-6797 HYPH denotes -
T14802 6797-6801 NN denotes PAGE
T14804 6801-6803 , denotes ,
T14805 6803-6806 CC denotes and
T14806 6807-6821 NN denotes immunoblotting
T14808 6822-6825 VBD denotes was
T14807 6826-6835 VBN denotes performed
T14809 6836-6841 VBG denotes using
T14810 6842-6852 NNS denotes antibodies
T14811 6853-6860 IN denotes against
T14812 6861-6864 NN denotes Akt
T14813 6864-6866 , denotes ,
T14814 6866-6867 NN denotes p
T14816 6867-6868 HYPH denotes -
T14815 6868-6871 NN denotes Akt
T14817 6872-6873 -LRB- denotes (
T14818 6873-6879 NN denotes Ser473
T14819 6879-6880 -RRB- denotes )
T14820 6880-6882 , denotes ,
T14821 6882-6886 NN denotes CREB
T14822 6886-6888 , denotes ,
T14823 6888-6889 NN denotes p
T14825 6889-6890 HYPH denotes -
T14824 6890-6894 NN denotes CREB
T14826 6895-6896 -LRB- denotes (
T14827 6896-6902 NN denotes Ser133
T14828 6902-6903 -RRB- denotes )
T14829 6903-6905 , denotes ,
T14830 6905-6908 NN denotes Bax
T14831 6908-6910 , denotes ,
T14832 6910-6913 NN denotes Bcl
T14834 6913-6914 HYPH denotes -
T14833 6914-6916 NN denotes xl
T14835 6917-6918 -LRB- denotes (
T14837 6918-6922 NNP denotes Cell
T14838 6923-6932 NNP denotes Signaling
T14836 6933-6943 NNP denotes Technology
T14839 6943-6945 , denotes ,
T14840 6945-6952 NNP denotes Beverly
T14841 6952-6954 , denotes ,
T14842 6954-6967 NNP denotes Massachusetts
T14843 6967-6969 , denotes ,
T14844 6969-6975 NNP denotes United
T14845 6976-6982 NNP denotes States
T14846 6982-6983 -RRB- denotes )
T14847 6983-6985 , denotes ,
T14848 6985-6988 CC denotes and
T14849 6989-6993 NN denotes Bcl2
T14850 6994-6995 -LRB- denotes (
T14852 6995-6997 NN denotes BD
T14851 6998-7008 NNP denotes Pharmingen
T14853 7008-7010 , denotes ,
T14854 7010-7013 NNP denotes San
T14855 7014-7019 NNP denotes Diego
T14856 7019-7021 , denotes ,
T14857 7021-7031 NNP denotes California
T14858 7031-7033 , denotes ,
T14859 7033-7039 NNP denotes United
T14860 7040-7046 NNP denotes States
T14861 7046-7047 -RRB- denotes )
T14862 7047-7048 . denotes .
T14863 7048-7251 sentence denotes For quantification, films (X-OMAT AR, Eastman Kodak, Rochester, New York, United States) were scanned and densitometry was performed using IMAGEQUANT 5.2 (Molecular Dynamics, Amersham, Uppsala, Sweden).
T14864 7049-7052 IN denotes For
T14866 7053-7067 NN denotes quantification
T14867 7067-7069 , denotes ,
T14868 7069-7074 NNS denotes films
T14869 7075-7076 -LRB- denotes (
T14871 7076-7077 NN denotes X
T14873 7077-7078 HYPH denotes -
T14872 7078-7082 NN denotes OMAT
T14870 7083-7085 NNP denotes AR
T14874 7085-7087 , denotes ,
T14875 7087-7094 NNP denotes Eastman
T14876 7095-7100 NNP denotes Kodak
T14877 7100-7102 , denotes ,
T14878 7102-7111 NNP denotes Rochester
T14879 7111-7113 , denotes ,
T14880 7113-7116 NNP denotes New
T14881 7117-7121 NNP denotes York
T14882 7121-7123 , denotes ,
T14883 7123-7129 NNP denotes United
T14884 7130-7136 NNP denotes States
T14885 7136-7137 -RRB- denotes )
T14886 7138-7142 VBD denotes were
T14865 7143-7150 VBN denotes scanned
T14887 7151-7154 CC denotes and
T14888 7155-7167 NN denotes densitometry
T14890 7168-7171 VBD denotes was
T14889 7172-7181 VBN denotes performed
T14891 7182-7187 VBG denotes using
T14892 7188-7198 NNP denotes IMAGEQUANT
T14893 7199-7202 CD denotes 5.2
T14894 7203-7204 -LRB- denotes (
T14896 7204-7213 NNP denotes Molecular
T14895 7214-7222 NNP denotes Dynamics
T14897 7222-7224 , denotes ,
T14898 7224-7232 NNP denotes Amersham
T14899 7232-7234 , denotes ,
T14900 7234-7241 NNP denotes Uppsala
T14901 7241-7243 , denotes ,
T14902 7243-7249 NNP denotes Sweden
T14903 7249-7250 -RRB- denotes )
T14904 7250-7251 . denotes .
T15020 7253-7270 NN denotes Electrophysiology
T15021 7270-7344 sentence denotes Electrophysiological analysis was performed as previously described [48].
T15022 7271-7291 JJ denotes Electrophysiological
T15023 7292-7300 NN denotes analysis
T15025 7301-7304 VBD denotes was
T15024 7305-7314 VBN denotes performed
T15026 7315-7317 IN denotes as
T15028 7318-7328 RB denotes previously
T15027 7329-7338 VBN denotes described
T15029 7339-7340 -LRB- denotes [
T15030 7340-7342 CD denotes 48
T15031 7342-7343 -RRB- denotes ]
T15032 7343-7344 . denotes .
T15033 7344-7473 sentence denotes Briefly, intracellular recordings from identified quadriceps motor neurons were made using sharp electrodes (75–120 MΩ, 3M KCl).
T15034 7345-7352 RB denotes Briefly
T15036 7352-7354 , denotes ,
T15037 7354-7367 JJ denotes intracellular
T15038 7368-7378 NNS denotes recordings
T15039 7379-7383 IN denotes from
T15040 7384-7394 VBN denotes identified
T15042 7395-7405 NNS denotes quadriceps
T15043 7406-7411 NN denotes motor
T15041 7412-7419 NNS denotes neurons
T15044 7420-7424 VBD denotes were
T15035 7425-7429 VBN denotes made
T15045 7430-7435 VBG denotes using
T15046 7436-7441 JJ denotes sharp
T15047 7442-7452 NNS denotes electrodes
T15048 7453-7454 -LRB- denotes (
T15050 7454-7456 CD denotes 75
T15052 7456-7457 SYM denotes –
T15051 7457-7460 CD denotes 120
T15049 7461-7463 NN denotes MΩ
T15053 7463-7465 , denotes ,
T15054 7465-7467 NN denotes 3M
T15055 7468-7471 NN denotes KCl
T15056 7471-7472 -RRB- denotes )
T15057 7472-7473 . denotes .
T15058 7473-7673 sentence denotes Average responses (10–20 trials) from suprathreshold nerve stimulation (1.5 times the strength that evokes maximal monosynaptic response) of the quadriceps nerve were acquired with LTP software [49].
T15059 7474-7481 JJ denotes Average
T15060 7482-7491 NNS denotes responses
T15062 7492-7493 -LRB- denotes (
T15064 7493-7495 CD denotes 10
T15066 7495-7496 SYM denotes –
T15065 7496-7498 CD denotes 20
T15063 7499-7505 NNS denotes trials
T15067 7505-7506 -RRB- denotes )
T15068 7507-7511 IN denotes from
T15069 7512-7526 NN denotes suprathreshold
T15071 7527-7532 NN denotes nerve
T15070 7533-7544 NN denotes stimulation
T15072 7545-7546 -LRB- denotes (
T15074 7546-7549 CD denotes 1.5
T15075 7550-7555 NNS denotes times
T15076 7556-7559 DT denotes the
T15073 7560-7568 NN denotes strength
T15077 7569-7573 WDT denotes that
T15078 7574-7580 VBZ denotes evokes
T15079 7581-7588 JJ denotes maximal
T15081 7589-7601 JJ denotes monosynaptic
T15080 7602-7610 NN denotes response
T15082 7610-7611 -RRB- denotes )
T15083 7612-7614 IN denotes of
T15084 7615-7618 DT denotes the
T15086 7619-7629 NNS denotes quadriceps
T15085 7630-7635 NN denotes nerve
T15087 7636-7640 VBD denotes were
T15061 7641-7649 VBN denotes acquired
T15088 7650-7654 IN denotes with
T15089 7655-7658 NN denotes LTP
T15090 7659-7667 NN denotes software
T15091 7668-7669 -LRB- denotes [
T15092 7669-7671 CD denotes 49
T15093 7671-7672 -RRB- denotes ]
T15094 7672-7673 . denotes .
T15095 7673-7771 sentence denotes Only cells with stable resting potentials more negative than −50 mV were considered for analysis.
T15096 7674-7678 RB denotes Only
T15097 7679-7684 NNS denotes cells
T15099 7685-7689 IN denotes with
T15100 7690-7696 JJ denotes stable
T15102 7697-7704 NN denotes resting
T15101 7705-7715 NNS denotes potentials
T15103 7716-7720 RBR denotes more
T15104 7721-7729 JJ denotes negative
T15105 7730-7734 IN denotes than
T15106 7735-7736 SYM denotes −
T15107 7736-7738 CD denotes 50
T15108 7739-7741 NN denotes mV
T15109 7742-7746 VBD denotes were
T15098 7747-7757 VBN denotes considered
T15110 7758-7761 IN denotes for
T15111 7762-7770 NN denotes analysis
T15112 7770-7771 . denotes .
T15113 7771-7951 sentence denotes Monosynaptic amplitudes were determined offline using custom routines in the Matlab environment (The Mathworks, Natick, Massachusetts, United States) as previously described [48].
T15114 7772-7784 JJ denotes Monosynaptic
T15115 7785-7795 NNS denotes amplitudes
T15117 7796-7800 VBD denotes were
T15116 7801-7811 VBN denotes determined
T15118 7812-7819 RB denotes offline
T15119 7820-7825 VBG denotes using
T15120 7826-7832 NN denotes custom
T15121 7833-7841 NNS denotes routines
T15122 7842-7844 IN denotes in
T15123 7845-7848 DT denotes the
T15125 7849-7855 NNP denotes Matlab
T15124 7856-7867 NN denotes environment
T15126 7868-7869 -LRB- denotes (
T15128 7869-7872 DT denotes The
T15127 7873-7882 NNP denotes Mathworks
T15129 7882-7884 , denotes ,
T15130 7884-7890 NNP denotes Natick
T15131 7890-7892 , denotes ,
T15132 7892-7905 NNP denotes Massachusetts
T15133 7905-7907 , denotes ,
T15134 7907-7913 NNP denotes United
T15135 7914-7920 NNP denotes States
T15136 7920-7921 -RRB- denotes )
T15137 7922-7924 IN denotes as
T15139 7925-7935 RB denotes previously
T15138 7936-7945 VBN denotes described
T15140 7946-7947 -LRB- denotes [
T15141 7947-7949 CD denotes 48
T15142 7949-7950 -RRB- denotes ]
T15143 7950-7951 . denotes .
R5259 T12346 T12345 prep of,Generation
R5260 T12347 T12348 amod transgenic,mice
R5261 T12348 T12346 pobj mice,of
R5262 T12349 T12345 cc and,Generation
R5263 T12350 T12351 compound mouse,genetics
R5264 T12351 T12345 conj genetics,Generation
R5265 T12353 T12354 compound Er81EWS,Pea3
R5266 T12354 T12356 compound Pea3,mice
R5267 T12355 T12354 punct -,Pea3
R5268 T12356 T12357 nsubjpass mice,generated
R5269 T12358 T12357 auxpass were,generated
R5270 T12359 T12357 advcl following,generated
R5271 T12360 T12361 det a,strategy
R5272 T12361 T12359 dobj strategy,following
R5273 T12362 T12361 amod similar,strategy
R5274 T12363 T12362 prep to,similar
R5275 T12364 T12363 pobj that,to
R5276 T12365 T12364 acl described,that
R5277 T12366 T12365 prep for,described
R5278 T12367 T12368 det the,generation
R5279 T12368 T12366 pobj generation,for
R5280 T12369 T12368 prep of,generation
R5281 T12370 T12371 compound Er81NLZ,mice
R5282 T12371 T12369 pobj mice,of
R5283 T12372 T12373 punct [,14
R5284 T12373 T12365 parataxis 14,described
R5285 T12374 T12373 punct ],14
R5286 T12375 T12357 punct .,generated
R5287 T12377 T12378 prep In,inserted
R5288 T12379 T12377 pobj brief,In
R5289 T12380 T12378 punct ", ",inserted
R5290 T12381 T12382 det a,vector
R5291 T12382 T12378 nsubjpass vector,inserted
R5292 T12383 T12382 compound targeting,vector
R5293 T12384 T12382 prep with,vector
R5294 T12385 T12386 det a,coding
R5295 T12386 T12384 pobj coding,with
R5296 T12387 T12386 compound cDNA,coding
R5297 T12388 T12382 prep for,vector
R5298 T12389 T12390 compound EWS,Pea3
R5299 T12390 T12388 pobj Pea3,for
R5300 T12391 T12390 punct -,Pea3
R5301 T12392 T12378 auxpass was,inserted
R5302 T12393 T12378 prep in,inserted
R5303 T12394 T12393 pobj frame,in
R5304 T12395 T12394 prep with,frame
R5305 T12396 T12397 det the,ATG
R5306 T12397 T12395 pobj ATG,with
R5307 T12398 T12397 amod endogenous,ATG
R5308 T12399 T12397 compound start,ATG
R5309 T12400 T12378 prep into,inserted
R5310 T12401 T12400 pobj exon,into
R5311 T12402 T12401 nummod 2,exon
R5312 T12403 T12401 prep of,exon
R5313 T12404 T12405 det the,locus
R5314 T12405 T12403 pobj locus,of
R5315 T12406 T12405 nmod Er81,locus
R5316 T12407 T12405 amod genomic,locus
R5317 T12408 T12378 cc and,inserted
R5318 T12409 T12378 conj used,inserted
R5319 T12410 T12409 prep for,used
R5320 T12411 T12412 amod homologous,recombination
R5321 T12412 T12410 pobj recombination,for
R5322 T12413 T12409 prep in,used
R5323 T12414 T12415 compound W95,cells
R5324 T12415 T12413 pobj cells,in
R5325 T12416 T12415 compound ES,cells
R5326 T12417 T12378 punct .,inserted
R5327 T12419 T12420 compound EWS,Pea3
R5328 T12420 T12422 nsubj Pea3,represents
R5329 T12421 T12420 punct -,Pea3
R5330 T12423 T12424 det a,gene
R5331 T12424 T12422 dobj gene,represents
R5332 T12425 T12424 compound fusion,gene
R5333 T12426 T12424 prep between,gene
R5334 T12427 T12428 det the,terminal
R5335 T12428 T12426 pobj terminal,between
R5336 T12429 T12428 compound amino,terminal
R5337 T12430 T12428 prep of,terminal
R5338 T12431 T12430 pobj EWS,of
R5339 T12432 T12428 cc and,terminal
R5340 T12433 T12434 det the,domain
R5341 T12434 T12428 conj domain,terminal
R5342 T12435 T12434 compound ETS,domain
R5343 T12436 T12434 prep of,domain
R5344 T12437 T12436 pobj Pea3,of
R5345 T12438 T12439 punct [,20
R5346 T12439 T12422 parataxis 20,represents
R5347 T12440 T12439 punct ],20
R5348 T12441 T12422 punct .,represents
R5349 T12443 T12444 det The,pair
R5350 T12444 T12446 nsubj pair,was
R5351 T12445 T12444 compound primer,pair
R5352 T12447 T12444 acl used,pair
R5353 T12448 T12449 aux to,detect
R5354 T12449 T12447 advcl detect,used
R5355 T12450 T12449 advmod specifically,detect
R5356 T12451 T12452 det the,allele
R5357 T12452 T12449 dobj allele,detect
R5358 T12453 T12454 compound Er81EWS,Pea3
R5359 T12454 T12452 compound Pea3,allele
R5360 T12455 T12454 punct -,Pea3
R5361 T12456 T12457 nummod 5,CAGCCACTGCACCTACAAGAC
R5362 T12457 T12446 attr CAGCCACTGCACCTACAAGAC,was
R5363 T12458 T12456 punct ′,5
R5364 T12459 T12457 punct -,CAGCCACTGCACCTACAAGAC
R5365 T12460 T12457 punct -,CAGCCACTGCACCTACAAGAC
R5366 T12461 T12457 nummod 3,CAGCCACTGCACCTACAAGAC
R5367 T12462 T12457 punct ′,CAGCCACTGCACCTACAAGAC
R5368 T12463 T12457 cc and,CAGCCACTGCACCTACAAGAC
R5369 T12464 T12465 nummod 5,CTTCCTGCTTGATGTCTCCTTC
R5370 T12465 T12457 conj CTTCCTGCTTGATGTCTCCTTC,CAGCCACTGCACCTACAAGAC
R5371 T12466 T12464 punct ′,5
R5372 T12467 T12465 punct -,CTTCCTGCTTGATGTCTCCTTC
R5373 T12468 T12465 punct -,CTTCCTGCTTGATGTCTCCTTC
R5374 T12469 T12465 nummod 3,CTTCCTGCTTGATGTCTCCTTC
R5375 T12470 T12465 punct ′,CTTCCTGCTTGATGTCTCCTTC
R5376 T12471 T12446 punct .,was
R5377 T12473 T12474 prep For,integrated
R5378 T12475 T12476 det the,generation
R5379 T12476 T12473 pobj generation,For
R5380 T12477 T12476 prep of,generation
R5381 T12478 T12479 nmod TaumGFP,mice
R5382 T12479 T12477 pobj mice,of
R5383 T12480 T12478 cc and,TaumGFP
R5384 T12481 T12482 compound TauEWS,Pea3
R5385 T12482 T12478 conj Pea3,TaumGFP
R5386 T12483 T12482 punct -,Pea3
R5387 T12484 T12474 punct ", ",integrated
R5388 T12485 T12486 nmod lox,pA
R5389 T12486 T12500 nmod pA,cassettes
R5390 T12487 T12486 punct -,pA
R5391 T12488 T12486 nmod STOP,pA
R5392 T12489 T12486 punct -,pA
R5393 T12490 T12486 nmod lox,pA
R5394 T12491 T12486 punct -,pA
R5395 T12492 T12486 nmod mGFP,pA
R5396 T12493 T12486 punct -,pA
R5397 T12494 T12486 nmod IRES,pA
R5398 T12495 T12486 punct -,pA
R5399 T12496 T12486 nmod NLS,pA
R5400 T12497 T12486 punct -,pA
R5401 T12498 T12486 nmod LacZ,pA
R5402 T12499 T12486 punct -,pA
R5403 T12500 T12474 nsubjpass cassettes,integrated
R5404 T12501 T12486 cc and,pA
R5405 T12502 T12503 compound lox,pA
R5406 T12503 T12486 conj pA,pA
R5407 T12504 T12503 punct -,pA
R5408 T12505 T12503 compound STOP,pA
R5409 T12506 T12503 punct -,pA
R5410 T12507 T12503 compound lox,pA
R5411 T12508 T12503 punct -,pA
R5412 T12509 T12503 compound EWS,pA
R5413 T12510 T12503 punct -,pA
R5414 T12511 T12503 compound Pea3,pA
R5415 T12512 T12503 punct -,pA
R5416 T12513 T12503 compound IRES,pA
R5417 T12514 T12503 punct -,pA
R5418 T12515 T12503 compound NLS,pA
R5419 T12516 T12503 punct -,pA
R5420 T12517 T12503 compound LacZ,pA
R5421 T12518 T12503 punct -,pA
R5422 T12519 T12500 compound targeting,cassettes
R5423 T12520 T12474 auxpass were,integrated
R5424 T12521 T12474 prep into,integrated
R5425 T12522 T12521 pobj exon,into
R5426 T12523 T12522 nummod 2,exon
R5427 T12524 T12522 prep of,exon
R5428 T12525 T12526 det the,locus
R5429 T12526 T12524 pobj locus,of
R5430 T12527 T12526 nmod Tau,locus
R5431 T12528 T12526 amod genomic,locus
R5432 T12529 T12530 punct (,removed
R5433 T12530 T12474 parataxis removed,integrated
R5434 T12531 T12532 det the,ATG
R5435 T12532 T12530 nsubjpass ATG,removed
R5436 T12533 T12532 amod endogenous,ATG
R5437 T12534 T12532 compound start,ATG
R5438 T12535 T12530 auxpass was,removed
R5439 T12536 T12530 prep in,removed
R5440 T12537 T12538 det the,vectors
R5441 T12538 T12536 pobj vectors,in
R5442 T12539 T12538 compound targeting,vectors
R5443 T12540 T12530 punct ;,removed
R5444 T12541 T12542 nsubj details,available
R5445 T12542 T12530 amod available,removed
R5446 T12543 T12542 prep upon,available
R5447 T12544 T12543 pobj request,upon
R5448 T12545 T12530 punct ),removed
R5449 T12546 T12474 punct .,integrated
R5450 T12548 T12549 nsubjpass mGFP,provided
R5451 T12550 T12549 auxpass was,provided
R5452 T12551 T12549 agent by,provided
R5453 T12552 T12553 compound P.,Caroni
R5454 T12553 T12551 pobj Caroni,by
R5455 T12554 T12555 punct [,25
R5456 T12555 T12549 parataxis 25,provided
R5457 T12556 T12555 punct ],25
R5458 T12557 T12549 punct .,provided
R5459 T12559 T12560 compound ES,recombinants
R5460 T12560 T12562 nsubjpass recombinants,screened
R5461 T12561 T12560 compound cell,recombinants
R5462 T12563 T12562 auxpass were,screened
R5463 T12564 T12562 prep by,screened
R5464 T12565 T12566 compound Southern,blot
R5465 T12566 T12567 compound blot,analysis
R5466 T12567 T12564 pobj analysis,by
R5467 T12568 T12562 advcl using,screened
R5468 T12569 T12570 det the,probe
R5469 T12570 T12568 dobj probe,using
R5470 T12571 T12568 prep in,using
R5471 T12572 T12573 det the,region
R5472 T12573 T12571 pobj region,in
R5473 T12574 T12573 nummod 5,region
R5474 T12575 T12574 punct ′,5
R5475 T12576 T12577 mark as,described
R5476 T12577 T12568 advcl described,using
R5477 T12578 T12577 advmod previously,described
R5478 T12579 T12580 punct [,41
R5479 T12580 T12577 parataxis 41,described
R5480 T12581 T12580 punct ],41
R5481 T12582 T12562 punct .,screened
R5482 T12584 T12585 nsubj Frequency,was
R5483 T12586 T12584 prep of,Frequency
R5484 T12587 T12586 pobj recombination,of
R5485 T12588 T12587 prep in,recombination
R5486 T12589 T12590 nummod 129,Ola
R5487 T12590 T12592 compound Ola,cells
R5488 T12591 T12590 punct /,Ola
R5489 T12592 T12588 pobj cells,in
R5490 T12593 T12592 compound ES,cells
R5491 T12594 T12595 advmod approximately,3
R5492 T12595 T12585 attr 3,was
R5493 T12596 T12595 quantmod 1,3
R5494 T12597 T12595 punct /,3
R5495 T12598 T12585 prep for,was
R5496 T12599 T12600 det both,constructs
R5497 T12600 T12598 pobj constructs,for
R5498 T12601 T12600 compound Tau,constructs
R5499 T12602 T12585 punct .,was
R5500 T12604 T12605 prep For,obtained
R5501 T12606 T12607 det the,generation
R5502 T12607 T12604 pobj generation,For
R5503 T12608 T12607 prep of,generation
R5504 T12609 T12610 compound PVCre,mice
R5505 T12610 T12608 pobj mice,of
R5506 T12611 T12605 punct ", ",obtained
R5507 T12612 T12613 nmod mouse,clones
R5508 T12613 T12605 nsubjpass clones,obtained
R5509 T12614 T12613 amod genomic,clones
R5510 T12615 T12605 auxpass were,obtained
R5511 T12616 T12605 prep by,obtained
R5512 T12617 T12616 pcomp screening,by
R5513 T12618 T12619 det a,library
R5514 T12619 T12617 dobj library,screening
R5515 T12620 T12621 nmod 129SV,J
R5516 T12621 T12619 nmod J,library
R5517 T12622 T12621 punct /,J
R5518 T12623 T12619 amod genomic,library
R5519 T12624 T12625 punct (,Incyte
R5520 T12625 T12617 parataxis Incyte,screening
R5521 T12626 T12625 punct ", ",Incyte
R5522 T12627 T12625 npadvmod Wilmington,Incyte
R5523 T12628 T12625 punct ", ",Incyte
R5524 T12629 T12625 npadvmod Delaware,Incyte
R5525 T12630 T12625 punct ", ",Incyte
R5526 T12631 T12632 compound United,States
R5527 T12632 T12625 npadvmod States,Incyte
R5528 T12633 T12625 punct ),Incyte
R5529 T12634 T12605 punct .,obtained
R5530 T12636 T12637 prep For,see
R5531 T12638 T12636 pobj details,For
R5532 T12639 T12638 prep on,details
R5533 T12640 T12641 det the,structure
R5534 T12641 T12639 pobj structure,on
R5535 T12642 T12641 amod genomic,structure
R5536 T12643 T12641 prep of,structure
R5537 T12644 T12645 det the,locus
R5538 T12645 T12643 pobj locus,of
R5539 T12646 T12645 compound mouse,locus
R5540 T12647 T12645 compound PV,locus
R5541 T12648 T12637 punct [,see
R5542 T12649 T12637 dobj 42,see
R5543 T12650 T12637 punct ],see
R5544 T12651 T12637 punct .,see
R5545 T12653 T12654 det An,cassette
R5546 T12654 T12661 nsubjpass cassette,integrated
R5547 T12655 T12656 compound IRES,pA
R5548 T12656 T12654 compound pA,cassette
R5549 T12657 T12656 punct -,pA
R5550 T12658 T12656 compound Cre,pA
R5551 T12659 T12656 punct -,pA
R5552 T12660 T12654 compound targeting,cassette
R5553 T12662 T12663 punct [,33
R5554 T12663 T12654 parataxis 33,cassette
R5555 T12664 T12663 punct ],33
R5556 T12665 T12661 auxpass was,integrated
R5557 T12666 T12661 prep into,integrated
R5558 T12667 T12668 det the,UTR
R5559 T12668 T12666 pobj UTR,into
R5560 T12669 T12668 nummod 3,UTR
R5561 T12670 T12669 punct ′,3
R5562 T12671 T12668 prep of,UTR
R5563 T12672 T12671 pobj exon,of
R5564 T12673 T12672 nummod 5,exon
R5565 T12674 T12661 punct ", ",integrated
R5566 T12675 T12661 cc and,integrated
R5567 T12676 T12677 compound ES,recombinants
R5568 T12677 T12679 nsubjpass recombinants,screened
R5569 T12678 T12677 compound cell,recombinants
R5570 T12679 T12661 conj screened,integrated
R5571 T12680 T12679 auxpass were,screened
R5572 T12681 T12679 prep with,screened
R5573 T12682 T12683 det a,probe
R5574 T12683 T12681 pobj probe,with
R5575 T12684 T12683 nummod 5,probe
R5576 T12685 T12684 punct ′,5
R5577 T12686 T12687 punct (,digest
R5578 T12687 T12679 parataxis digest,screened
R5579 T12688 T12687 dep oligos,digest
R5580 T12689 T12688 punct ", ",oligos
R5581 T12690 T12691 nummod 5,GAGATGACCCAGCCAGGATGCCTC
R5582 T12691 T12688 conj GAGATGACCCAGCCAGGATGCCTC,oligos
R5583 T12692 T12690 punct ′,5
R5584 T12693 T12691 punct -,GAGATGACCCAGCCAGGATGCCTC
R5585 T12694 T12691 punct -,GAGATGACCCAGCCAGGATGCCTC
R5586 T12695 T12691 nummod 3,GAGATGACCCAGCCAGGATGCCTC
R5587 T12696 T12691 punct ′,GAGATGACCCAGCCAGGATGCCTC
R5588 T12697 T12691 cc and,GAGATGACCCAGCCAGGATGCCTC
R5589 T12698 T12699 nummod 5,CTGACCACTCTCGCTCCGGTGTCC
R5590 T12699 T12691 conj CTGACCACTCTCGCTCCGGTGTCC,GAGATGACCCAGCCAGGATGCCTC
R5591 T12700 T12698 punct ′,5
R5592 T12701 T12699 punct -,CTGACCACTCTCGCTCCGGTGTCC
R5593 T12702 T12699 punct -,CTGACCACTCTCGCTCCGGTGTCC
R5594 T12703 T12699 nummod 3,CTGACCACTCTCGCTCCGGTGTCC
R5595 T12704 T12699 punct ′,CTGACCACTCTCGCTCCGGTGTCC
R5596 T12705 T12687 punct ;,digest
R5597 T12706 T12707 amod genomic,DNA
R5598 T12707 T12687 dep DNA,digest
R5599 T12708 T12687 punct ", ",digest
R5600 T12709 T12687 compound HindIII,digest
R5601 T12710 T12687 punct ),digest
R5602 T12711 T12679 punct .,screened
R5603 T12713 T12714 det The,frequency
R5604 T12714 T12715 nsubj frequency,was
R5605 T12716 T12714 prep of,frequency
R5606 T12717 T12716 pobj recombination,of
R5607 T12718 T12717 prep in,recombination
R5608 T12719 T12720 nummod 129,Ola
R5609 T12720 T12722 compound Ola,cells
R5610 T12721 T12720 punct /,Ola
R5611 T12722 T12718 pobj cells,in
R5612 T12723 T12722 compound ES,cells
R5613 T12724 T12725 advmod approximately,20
R5614 T12725 T12715 attr 20,was
R5615 T12726 T12725 quantmod 1,20
R5616 T12727 T12725 punct /,20
R5617 T12728 T12715 punct .,was
R5618 T12730 T12731 amod Recombinant,clones
R5619 T12731 T12732 nsubjpass clones,aggregated
R5620 T12733 T12732 auxpass were,aggregated
R5621 T12734 T12732 prep with,aggregated
R5622 T12735 T12736 compound morula,stage
R5623 T12736 T12737 compound stage,embryos
R5624 T12737 T12734 pobj embryos,with
R5625 T12738 T12739 aux to,generate
R5626 T12739 T12732 advcl generate,aggregated
R5627 T12740 T12741 amod chimeric,mice
R5628 T12741 T12739 dobj mice,generate
R5629 T12742 T12741 compound founder,mice
R5630 T12743 T12744 dep that,transmitted
R5631 T12744 T12741 relcl transmitted,mice
R5632 T12745 T12746 det the,alleles
R5633 T12746 T12744 dobj alleles,transmitted
R5634 T12747 T12746 compound mutant,alleles
R5635 T12748 T12732 punct .,aggregated
R5636 T12750 T12751 prep In,were
R5637 T12752 T12753 det all,experiments
R5638 T12753 T12750 pobj experiments,In
R5639 T12754 T12753 acl performed,experiments
R5640 T12755 T12754 prep in,performed
R5641 T12756 T12757 det this,study
R5642 T12757 T12755 pobj study,in
R5643 T12758 T12751 punct ", ",were
R5644 T12759 T12751 nsubj animals,were
R5645 T12760 T12751 prep of,were
R5646 T12761 T12762 amod mixed,background
R5647 T12762 T12760 pobj background,of
R5648 T12763 T12762 amod genetic,background
R5649 T12764 T12765 punct (,Ola
R5650 T12765 T12751 parataxis Ola,were
R5651 T12766 T12765 nummod 129,Ola
R5652 T12767 T12765 punct /,Ola
R5653 T12768 T12765 cc and,Ola
R5654 T12769 T12765 conj C57Bl6,Ola
R5655 T12770 T12765 punct ),Ola
R5656 T12771 T12751 punct .,were
R5657 T12773 T12774 nmod Thy1spGFP,mice
R5658 T12774 T12776 nsubjpass mice,generated
R5659 T12775 T12774 amod transgenic,mice
R5660 T12777 T12776 auxpass were,generated
R5661 T12778 T12776 prep in,generated
R5662 T12779 T12778 pobj analogy,in
R5663 T12780 T12779 prep to,analogy
R5664 T12781 T12782 compound De,Paola
R5665 T12782 T12780 pobj Paola,to
R5666 T12783 T12784 advmod et,al.
R5667 T12784 T12782 advmod al.,Paola
R5668 T12785 T12786 punct [,25
R5669 T12786 T12776 parataxis 25,generated
R5670 T12787 T12786 punct ],25
R5671 T12788 T12776 punct ", ",generated
R5672 T12789 T12776 cc and,generated
R5673 T12790 T12791 prep for,selected
R5674 T12791 T12776 conj selected,generated
R5675 T12792 T12793 det these,experiments
R5676 T12793 T12790 pobj experiments,for
R5677 T12794 T12795 det a,strain
R5678 T12795 T12791 nsubjpass strain,selected
R5679 T12796 T12795 prep of,strain
R5680 T12797 T12796 pobj mice,of
R5681 T12798 T12797 prep with,mice
R5682 T12799 T12800 amod early,expression
R5683 T12800 T12798 pobj expression,with
R5684 T12801 T12800 amod embryonic,expression
R5685 T12802 T12791 auxpass was,selected
R5686 T12803 T12791 punct .,selected
R5687 T12805 T12806 nmod Isl1Cre,strains
R5688 T12806 T12810 nsubjpass strains,described
R5689 T12807 T12805 cc and,Isl1Cre
R5690 T12808 T12805 conj Hb9Cre,Isl1Cre
R5691 T12809 T12806 compound mouse,strains
R5692 T12811 T12810 aux have,described
R5693 T12812 T12810 auxpass been,described
R5694 T12813 T12814 punct [,43
R5695 T12814 T12810 parataxis 43,described
R5696 T12815 T12814 nummod 33,43
R5697 T12816 T12814 punct ",",43
R5698 T12817 T12814 punct ],43
R5699 T12818 T12810 cc and,described
R5700 T12819 T12820 nmod Bax,animals
R5701 T12820 T12824 nsubj animals,were
R5702 T12821 T12819 punct +,Bax
R5703 T12822 T12819 punct /,Bax
R5704 T12823 T12819 punct −,Bax
R5705 T12824 T12810 conj were,described
R5706 T12825 T12824 prep from,were
R5707 T12826 T12827 compound Jackson,Laboratory
R5708 T12827 T12825 pobj Laboratory,from
R5709 T12828 T12829 punct (,Harbor
R5710 T12829 T12824 parataxis Harbor,were
R5711 T12830 T12829 compound Bar,Harbor
R5712 T12831 T12829 punct ", ",Harbor
R5713 T12832 T12829 npadvmod Maine,Harbor
R5714 T12833 T12829 punct ", ",Harbor
R5715 T12834 T12835 compound United,States
R5716 T12835 T12829 npadvmod States,Harbor
R5717 T12836 T12829 punct ),Harbor
R5718 T12837 T12838 punct [,27
R5719 T12838 T12824 parataxis 27,were
R5720 T12839 T12838 punct ],27
R5721 T12840 T12824 punct .,were
R5722 T12842 T12843 amod Timed,pregnancies
R5723 T12843 T12844 nsubjpass pregnancies,set
R5724 T12845 T12844 auxpass were,set
R5725 T12846 T12844 prt up,set
R5726 T12847 T12848 aux to,generate
R5727 T12848 T12844 advcl generate,set
R5728 T12849 T12848 dobj embryos,generate
R5729 T12850 T12849 prep of,embryos
R5730 T12851 T12852 amod different,stages
R5731 T12852 T12850 pobj stages,of
R5732 T12853 T12852 amod developmental,stages
R5733 T12854 T12855 mark with,described
R5734 T12855 T12844 advcl described,set
R5735 T12856 T12857 det all,genotypes
R5736 T12857 T12855 nsubj genotypes,described
R5737 T12858 T12855 prep throughout,described
R5738 T12859 T12860 det the,study
R5739 T12860 T12858 pobj study,throughout
R5740 T12861 T12844 punct .,set
R5741 T13099 T13100 amod Transcriptional,transactivation
R5742 T13100 T13101 compound transactivation,assays
R5743 T13103 T13104 det The,plasmids
R5744 T13104 T13106 nsubjpass plasmids,used
R5745 T13105 T13104 amod following,plasmids
R5746 T13107 T13106 auxpass were,used
R5747 T13108 T13106 prep for,used
R5748 T13109 T13110 amod transcriptional,assays
R5749 T13110 T13108 pobj assays,for
R5750 T13111 T13110 compound transactivation,assays
R5751 T13112 T13106 punct : ,used
R5752 T13113 T13114 compound pRc,RSV
R5753 T13114 T13106 dobj RSV,used
R5754 T13115 T13114 punct /,RSV
R5755 T13116 T13117 punct (,Invitrogen
R5756 T13117 T13114 parataxis Invitrogen,RSV
R5757 T13118 T13117 punct ", ",Invitrogen
R5758 T13119 T13117 npadvmod Carlsbad,Invitrogen
R5759 T13120 T13117 punct ", ",Invitrogen
R5760 T13121 T13117 npadvmod California,Invitrogen
R5761 T13122 T13117 punct ", ",Invitrogen
R5762 T13123 T13124 compound United,States
R5763 T13124 T13117 npadvmod States,Invitrogen
R5764 T13125 T13117 punct ),Invitrogen
R5765 T13126 T13114 punct ", ",RSV
R5766 T13127 T13128 compound pRc,RSV
R5767 T13128 T13130 compound RSV,Er81
R5768 T13129 T13128 punct /,RSV
R5769 T13130 T13114 conj Er81,RSV
R5770 T13131 T13130 punct -,Er81
R5771 T13132 T13130 punct ", ",Er81
R5772 T13133 T13134 compound pRc,RSV
R5773 T13134 T13136 compound RSV,Pea3
R5774 T13135 T13134 punct /,RSV
R5775 T13136 T13130 conj Pea3,Er81
R5776 T13137 T13136 punct -,Pea3
R5777 T13138 T13136 compound EWS,Pea3
R5778 T13139 T13136 punct -,Pea3
R5779 T13140 T13136 punct ", ",Pea3
R5780 T13141 T13142 compound pTP,5xETS
R5781 T13142 T13136 conj 5xETS,Pea3
R5782 T13143 T13142 punct -,5xETS
R5783 T13144 T13142 punct ", ",5xETS
R5784 T13145 T13142 cc and,5xETS
R5785 T13146 T13147 compound pTP,5xETSmut
R5786 T13147 T13142 conj 5xETSmut,5xETS
R5787 T13148 T13147 punct -,5xETSmut
R5788 T13149 T13106 punct .,used
R5789 T13151 T13152 compound pRc,RSV
R5790 T13152 T13154 compound RSV,Er81
R5791 T13153 T13152 punct /,RSV
R5792 T13154 T13156 nsubjpass Er81,obtained
R5793 T13155 T13154 punct -,Er81
R5794 T13157 T13154 cc and,Er81
R5795 T13158 T13159 compound pRc,RSV
R5796 T13159 T13161 compound RSV,Pea3
R5797 T13160 T13159 punct /,RSV
R5798 T13161 T13154 conj Pea3,Er81
R5799 T13162 T13161 punct -,Pea3
R5800 T13163 T13161 compound EWS,Pea3
R5801 T13164 T13161 punct -,Pea3
R5802 T13165 T13156 auxpass were,obtained
R5803 T13166 T13156 prep by,obtained
R5804 T13167 T13166 pobj insertion,by
R5805 T13168 T13167 prep of,insertion
R5806 T13169 T13170 det the,cDNAs
R5807 T13170 T13168 pobj cDNAs,of
R5808 T13171 T13170 prep for,cDNAs
R5809 T13172 T13171 pobj Er81,for
R5810 T13173 T13172 cc or,Er81
R5811 T13174 T13175 compound EWS,Pea3
R5812 T13175 T13172 conj Pea3,Er81
R5813 T13176 T13175 punct -,Pea3
R5814 T13177 T13178 punct (,gift
R5815 T13178 T13175 parataxis gift,Pea3
R5816 T13179 T13178 prep from,gift
R5817 T13180 T13181 compound J.,Hassell
R5818 T13181 T13179 pobj Hassell,from
R5819 T13182 T13181 compound A.,Hassell
R5820 T13183 T13178 punct ),gift
R5821 T13184 T13167 prep into,insertion
R5822 T13185 T13186 compound pRc,RSV
R5823 T13186 T13184 pobj RSV,into
R5824 T13187 T13186 punct /,RSV
R5825 T13188 T13156 punct .,obtained
R5826 T13190 T13191 compound pTP,5xETS
R5827 T13191 T13193 nsubjpass 5xETS,constructed
R5828 T13192 T13191 punct -,5xETS
R5829 T13194 T13193 auxpass was,constructed
R5830 T13195 T13193 prep by,constructed
R5831 T13196 T13195 pcomp inserting,by
R5832 T13197 T13198 det a,cassette
R5833 T13198 T13196 dobj cassette,inserting
R5834 T13199 T13198 prep of,cassette
R5835 T13200 T13201 nummod five,copies
R5836 T13201 T13199 pobj copies,of
R5837 T13202 T13201 amod repetitive,copies
R5838 T13203 T13201 prep of,copies
R5839 T13204 T13205 amod high,affinity
R5840 T13205 T13207 compound affinity,sites
R5841 T13206 T13205 punct -,affinity
R5842 T13207 T13203 pobj sites,of
R5843 T13208 T13207 compound Pea3,sites
R5844 T13209 T13207 compound binding,sites
R5845 T13210 T13211 punct (,3
R5846 T13211 T13207 parataxis 3,sites
R5847 T13212 T13211 dep 5,3
R5848 T13213 T13212 punct ′,5
R5849 T13214 T13211 punct -,3
R5850 T13215 T13211 dep GCCGGAAGC,3
R5851 T13216 T13211 punct -,3
R5852 T13217 T13211 punct ′,3
R5853 T13218 T13211 punct ),3
R5854 T13219 T13220 punct [,19
R5855 T13220 T13207 parataxis 19,sites
R5856 T13221 T13220 nummod 18,19
R5857 T13222 T13220 punct ",",19
R5858 T13223 T13220 punct ],19
R5859 T13224 T13196 prep into,inserting
R5860 T13225 T13226 det a,version
R5861 T13226 T13224 pobj version,into
R5862 T13227 T13226 amod modified,version
R5863 T13228 T13226 prep of,version
R5864 T13229 T13230 compound pTK,Luc
R5865 T13230 T13228 pobj Luc,of
R5866 T13231 T13230 punct -,Luc
R5867 T13232 T13193 punct .,constructed
R5868 T13234 T13235 compound pTP,5xETSmut
R5869 T13235 T13237 nsubjpass 5xETSmut,generated
R5870 T13236 T13235 punct -,5xETSmut
R5871 T13238 T13237 auxpass was,generated
R5872 T13239 T13237 prep as,generated
R5873 T13240 T13241 compound pTP,5xETS
R5874 T13241 T13239 pobj 5xETS,as
R5875 T13242 T13241 punct -,5xETS
R5876 T13243 T13239 cc but,as
R5877 T13244 T13239 conj using,as
R5878 T13245 T13246 det a,complement
R5879 T13246 T13244 dobj complement,using
R5880 T13247 T13246 amod mutated,complement
R5881 T13248 T13246 prep of,complement
R5882 T13249 T13250 det the,sites
R5883 T13250 T13248 pobj sites,of
R5884 T13251 T13250 compound Pea3,sites
R5885 T13252 T13250 compound binding,sites
R5886 T13253 T13254 punct (,3
R5887 T13254 T13246 parataxis 3,complement
R5888 T13255 T13254 dep 5,3
R5889 T13256 T13255 punct ′,5
R5890 T13257 T13254 punct -,3
R5891 T13258 T13254 dep GCCTATGGC,3
R5892 T13259 T13254 punct -,3
R5893 T13260 T13254 punct ′,3
R5894 T13261 T13254 punct ),3
R5895 T13262 T13237 punct .,generated
R5896 T13264 T13265 det A,plasmid
R5897 T13265 T13267 nsubj plasmid,were
R5898 T13266 T13265 compound control,plasmid
R5899 T13268 T13269 aux to,normalize
R5900 T13269 T13265 advcl normalize,plasmid
R5901 T13270 T13269 prep for,normalize
R5902 T13271 T13272 compound transfection,efficiency
R5903 T13272 T13270 pobj efficiency,for
R5904 T13273 T13274 punct (,placZ
R5905 T13274 T13272 parataxis placZ,efficiency
R5906 T13275 T13274 punct ),placZ
R5907 T13276 T13272 cc and,efficiency
R5908 T13277 T13278 compound pTK,Luc
R5909 T13278 T13272 conj Luc,efficiency
R5910 T13279 T13278 punct -,Luc
R5911 T13280 T13281 det a,gift
R5912 T13281 T13267 attr gift,were
R5913 T13282 T13281 prep from,gift
R5914 T13283 T13284 compound D.,Kressler
R5915 T13284 T13282 pobj Kressler,from
R5916 T13285 T13267 punct .,were
R5917 T13287 T13288 nmod COS,cells
R5918 T13288 T13291 nsubjpass cells,co-transfected
R5919 T13289 T13287 punct -,COS
R5920 T13290 T13287 nummod 7,COS
R5921 T13292 T13291 auxpass were,co-transfected
R5922 T13293 T13291 prep with,co-transfected
R5923 T13294 T13295 quantmod 1,1.2
R5924 T13295 T13297 nummod 1.2,μg
R5925 T13296 T13295 punct –,1.2
R5926 T13297 T13293 pobj μg,with
R5927 T13298 T13297 prep of,μg
R5928 T13299 T13300 amod total,DNA
R5929 T13300 T13298 pobj DNA,of
R5930 T13301 T13297 prep including,μg
R5931 T13302 T13301 pobj one,including
R5932 T13303 T13302 prep of,one
R5933 T13304 T13305 det the,plasmids
R5934 T13305 T13303 pobj plasmids,of
R5935 T13306 T13305 compound effector,plasmids
R5936 T13307 T13308 compound pRc,RSV
R5937 T13308 T13302 appos RSV,one
R5938 T13309 T13308 punct /,RSV
R5939 T13310 T13308 punct -,RSV
R5940 T13311 T13308 amod empty,RSV
R5941 T13312 T13308 punct ", ",RSV
R5942 T13313 T13314 compound pRc,RSV
R5943 T13314 T13316 compound RSV,Er81
R5944 T13315 T13314 punct /,RSV
R5945 T13316 T13308 conj Er81,RSV
R5946 T13317 T13316 punct -,Er81
R5947 T13318 T13316 punct ", ",Er81
R5948 T13319 T13316 cc or,Er81
R5949 T13320 T13321 compound pRc,RSV
R5950 T13321 T13323 compound RSV,Pea3
R5951 T13322 T13321 punct /,RSV
R5952 T13323 T13316 conj Pea3,Er81
R5953 T13324 T13323 punct -,Pea3
R5954 T13325 T13323 compound EWS,Pea3
R5955 T13326 T13323 punct -,Pea3
R5956 T13327 T13302 punct ;,one
R5957 T13328 T13302 conj one,one
R5958 T13329 T13328 prep of,one
R5959 T13330 T13331 det the,plasmids
R5960 T13331 T13329 pobj plasmids,of
R5961 T13332 T13331 compound reporter,plasmids
R5962 T13333 T13334 compound pTP,5xETS
R5963 T13334 T13328 appos 5xETS,one
R5964 T13335 T13334 punct -,5xETS
R5965 T13336 T13334 cc or,5xETS
R5966 T13337 T13338 compound pTP,5xETSmut
R5967 T13338 T13334 conj 5xETSmut,5xETS
R5968 T13339 T13338 punct -,5xETSmut
R5969 T13340 T13328 punct ;,one
R5970 T13341 T13328 cc and,one
R5971 T13342 T13328 conj placZ,one
R5972 T13343 T13291 punct .,co-transfected
R5973 T13345 T13346 nsubjpass Cells,harvested
R5974 T13347 T13346 auxpass were,harvested
R5975 T13348 T13346 prep after,harvested
R5976 T13349 T13350 nummod 25,h
R5977 T13350 T13348 pobj h,after
R5978 T13351 T13346 cc and,harvested
R5979 T13352 T13346 conj processed,harvested
R5980 T13353 T13352 prep for,processed
R5981 T13354 T13353 pobj assays,for
R5982 T13355 T13356 aux to,determine
R5983 T13356 T13352 advcl determine,processed
R5984 T13357 T13358 nmod luciferase,activity
R5985 T13358 T13356 dobj activity,determine
R5986 T13359 T13357 cc and,luciferase
R5987 T13360 T13357 conj LacZ,luciferase
R5988 T13361 T13362 mark as,described
R5989 T13362 T13356 advcl described,determine
R5990 T13363 T13362 advmod previously,described
R5991 T13364 T13365 punct [,44
R5992 T13365 T13352 parataxis 44,processed
R5993 T13366 T13365 punct ],44
R5994 T13367 T13346 punct .,harvested
R5995 T13369 T13370 compound Luciferase,values
R5996 T13370 T13371 nsubjpass values,referred
R5997 T13372 T13370 acl normalized,values
R5998 T13373 T13372 prep to,normalized
R5999 T13374 T13375 compound LacZ,activity
R6000 T13375 T13373 pobj activity,to
R6001 T13376 T13371 auxpass are,referred
R6002 T13377 T13371 prep to,referred
R6003 T13378 T13371 prep as,referred
R6004 T13379 T13380 compound luciferase,units
R6005 T13380 T13378 pobj units,as
R6006 T13381 T13371 punct .,referred
R6013 T13917 T13918 advmod In,situ
R6014 T13918 T13919 amod situ,hybridization
R6015 T13920 T13919 cc and,hybridization
R6016 T13921 T13919 conj immunohistochemistry,hybridization
R6017 T13923 T13924 prep For,hybridized
R6018 T13925 T13926 advmod in,situ
R6019 T13926 T13927 amod situ,analysis
R6020 T13927 T13923 pobj analysis,For
R6021 T13928 T13927 compound hybridization,analysis
R6022 T13929 T13924 punct ", ",hybridized
R6023 T13930 T13931 compound cryostat,sections
R6024 T13931 T13924 nsubjpass sections,hybridized
R6025 T13932 T13924 auxpass were,hybridized
R6026 T13933 T13924 advcl using,hybridized
R6027 T13934 T13935 npadvmod digoxigenin,labeled
R6028 T13935 T13937 amod labeled,probes
R6029 T13936 T13935 punct -,labeled
R6030 T13937 T13933 dobj probes,using
R6031 T13938 T13939 punct [,45
R6032 T13939 T13937 parataxis 45,probes
R6033 T13940 T13939 punct ],45
R6034 T13941 T13937 acl directed,probes
R6035 T13942 T13941 prep against,directed
R6036 T13943 T13944 compound mouse,TrkA
R6037 T13944 T13942 pobj TrkA,against
R6038 T13945 T13944 cc or,TrkA
R6039 T13946 T13944 conj TrkB,TrkA
R6040 T13947 T13944 punct ", ",TrkA
R6041 T13948 T13944 cc or,TrkA
R6042 T13949 T13950 compound rat,TrkC
R6043 T13950 T13944 conj TrkC,TrkA
R6044 T13951 T13952 punct (,gift
R6045 T13952 T13950 parataxis gift,TrkC
R6046 T13953 T13952 prep from,gift
R6047 T13954 T13955 compound L.,Parada
R6048 T13955 T13953 pobj Parada,from
R6049 T13956 T13955 compound F.,Parada
R6050 T13957 T13952 punct ),gift
R6051 T13958 T13924 punct .,hybridized
R6052 T13960 T13961 nsubj Antibodies,were
R6053 T13962 T13960 acl used,Antibodies
R6054 T13963 T13962 prep in,used
R6055 T13964 T13965 det this,study
R6056 T13965 T13963 pobj study,in
R6057 T13966 T13967 mark as,follows
R6058 T13967 T13961 advcl follows,were
R6059 T13968 T13961 punct : ,were
R6060 T13969 T13970 compound rabbit,anti-Er81
R6061 T13970 T13961 dep anti-Er81,were
R6062 T13971 T13972 punct [,14
R6063 T13972 T13970 parataxis 14,anti-Er81
R6064 T13973 T13972 punct ],14
R6065 T13974 T13970 punct ", ",anti-Er81
R6066 T13975 T13970 conj rabbit,anti-Er81
R6067 T13976 T13975 amod anti-Pea3,rabbit
R6068 T13977 T13978 punct [,14
R6069 T13978 T13975 parataxis 14,rabbit
R6070 T13979 T13978 punct ],14
R6071 T13980 T13975 punct ", ",rabbit
R6072 T13981 T13975 conj rabbit,rabbit
R6073 T13982 T13981 amod anti-PV,rabbit
R6074 T13983 T13984 punct [,14
R6075 T13984 T13981 parataxis 14,rabbit
R6076 T13985 T13984 punct ],14
R6077 T13986 T13981 punct ", ",rabbit
R6078 T13987 T13981 conj rabbit,rabbit
R6079 T13988 T13987 amod anti-eGFP,rabbit
R6080 T13989 T13990 punct (,Probes
R6081 T13990 T13987 parataxis Probes,rabbit
R6082 T13991 T13990 compound Molecular,Probes
R6083 T13992 T13990 punct ", ",Probes
R6084 T13993 T13990 npadvmod Eugene,Probes
R6085 T13994 T13990 punct ", ",Probes
R6086 T13995 T13990 npadvmod Oregon,Probes
R6087 T13996 T13990 punct ", ",Probes
R6088 T13997 T13998 compound United,States
R6089 T13998 T13990 npadvmod States,Probes
R6090 T13999 T13990 punct ),Probes
R6091 T14000 T13987 punct ", ",rabbit
R6092 T14001 T13987 conj rabbit,rabbit
R6093 T14002 T14001 amod anti-Calbindin,rabbit
R6094 T14003 T14001 punct ", ",rabbit
R6095 T14004 T14001 conj rabbit,rabbit
R6096 T14005 T14004 amod anti-Calretinin,rabbit
R6097 T14006 T14007 punct (,Swant
R6098 T14007 T14004 parataxis Swant,rabbit
R6099 T14008 T14007 punct ", ",Swant
R6100 T14009 T14007 npadvmod Bellinzona,Swant
R6101 T14010 T14007 punct ", ",Swant
R6102 T14011 T14007 npadvmod Switzerland,Swant
R6103 T14012 T14007 punct ),Swant
R6104 T14013 T14004 punct ", ",rabbit
R6105 T14014 T14004 conj rabbit,rabbit
R6106 T14015 T14014 amod anti-CGRP,rabbit
R6107 T14016 T14017 punct (,Chemicon
R6108 T14017 T14014 parataxis Chemicon,rabbit
R6109 T14018 T14017 punct ", ",Chemicon
R6110 T14019 T14017 npadvmod Temecula,Chemicon
R6111 T14020 T14017 punct ", ",Chemicon
R6112 T14021 T14017 npadvmod California,Chemicon
R6113 T14022 T14017 punct ", ",Chemicon
R6114 T14023 T14024 compound United,States
R6115 T14024 T14017 npadvmod States,Chemicon
R6116 T14025 T14017 punct ),Chemicon
R6117 T14026 T14014 punct ", ",rabbit
R6118 T14027 T14014 conj rabbit,rabbit
R6119 T14028 T14027 amod anti-vGlut1,rabbit
R6120 T14029 T14030 punct (,Systems
R6121 T14030 T14027 parataxis Systems,rabbit
R6122 T14031 T14030 compound Synaptic,Systems
R6123 T14032 T14030 punct ", ",Systems
R6124 T14033 T14030 npadvmod Goettingen,Systems
R6125 T14034 T14030 punct ", ",Systems
R6126 T14035 T14030 npadvmod Germany,Systems
R6127 T14036 T14030 punct ),Systems
R6128 T14037 T14027 punct ", ",rabbit
R6129 T14038 T14027 conj rabbit,rabbit
R6130 T14039 T14038 amod anti-Brn3a,rabbit
R6131 T14040 T14041 punct (,gift
R6132 T14041 T14038 parataxis gift,rabbit
R6133 T14042 T14041 prep from,gift
R6134 T14043 T14044 compound E.,Turner
R6135 T14044 T14042 pobj Turner,from
R6136 T14045 T14041 punct ),gift
R6137 T14046 T14038 punct ", ",rabbit
R6138 T14047 T14048 nmod rabbit,p75
R6139 T14048 T14038 conj p75,rabbit
R6140 T14049 T14048 amod anti-TrkA,p75
R6141 T14050 T14048 cc and,p75
R6142 T14051 T14048 punct -,p75
R6143 T14052 T14053 punct (,gift
R6144 T14053 T14048 parataxis gift,p75
R6145 T14054 T14053 prep from,gift
R6146 T14055 T14056 compound L.,Reichardt
R6147 T14056 T14054 pobj Reichardt,from
R6148 T14057 T14056 compound F.,Reichardt
R6149 T14058 T14053 punct ),gift
R6150 T14059 T14048 punct ", ",p75
R6151 T14060 T14048 conj rabbit,p75
R6152 T14061 T14060 amod anti-Runx3,rabbit
R6153 T14062 T14063 punct (,Kramer
R6154 T14063 T14060 parataxis Kramer,rabbit
R6155 T14064 T14063 cc and,Kramer
R6156 T14065 T14063 conj Arber,Kramer
R6157 T14066 T14063 punct ", ",Kramer
R6158 T14067 T14068 amod unpublished,reagent
R6159 T14068 T14063 npadvmod reagent,Kramer
R6160 T14069 T14063 punct ),Kramer
R6161 T14070 T14060 punct ", ",rabbit
R6162 T14071 T14060 conj rabbit,rabbit
R6163 T14072 T14071 amod anti-Rhodamine,rabbit
R6164 T14073 T14074 punct (,Probes
R6165 T14074 T14071 parataxis Probes,rabbit
R6166 T14075 T14074 compound Molecular,Probes
R6167 T14076 T14074 punct ),Probes
R6168 T14077 T14071 punct ", ",rabbit
R6169 T14078 T14071 conj mouse,rabbit
R6170 T14079 T14078 amod anti-neurofilament,mouse
R6171 T14080 T14081 punct (,Collection
R6172 T14081 T14078 parataxis Collection,mouse
R6173 T14082 T14081 compound American,Collection
R6174 T14083 T14081 compound Type,Collection
R6175 T14084 T14081 compound Culture,Collection
R6176 T14085 T14081 punct ", ",Collection
R6177 T14086 T14081 npadvmod Manassas,Collection
R6178 T14087 T14081 punct ", ",Collection
R6179 T14088 T14081 npadvmod Virginia,Collection
R6180 T14089 T14081 punct ", ",Collection
R6181 T14090 T14091 compound United,States
R6182 T14091 T14081 npadvmod States,Collection
R6183 T14092 T14081 punct ),Collection
R6184 T14093 T14078 punct ", ",mouse
R6185 T14094 T14078 conj sheep,mouse
R6186 T14095 T14094 amod anti-eGFP,sheep
R6187 T14096 T14097 punct (,Biogenesis
R6188 T14097 T14094 parataxis Biogenesis,sheep
R6189 T14098 T14097 punct ", ",Biogenesis
R6190 T14099 T14097 npadvmod Poole,Biogenesis
R6191 T14100 T14097 punct ", ",Biogenesis
R6192 T14101 T14102 compound United,Kingdom
R6193 T14102 T14097 npadvmod Kingdom,Biogenesis
R6194 T14103 T14097 punct ),Biogenesis
R6195 T14104 T14094 punct ", ",sheep
R6196 T14105 T14094 conj goat,sheep
R6197 T14106 T14105 amod anti-LacZ,goat
R6198 T14107 T14108 punct [,14
R6199 T14108 T14105 parataxis 14,goat
R6200 T14109 T14108 punct ],14
R6201 T14110 T14105 punct ", ",goat
R6202 T14111 T14105 conj goat,goat
R6203 T14112 T14111 amod anti-TrkC,goat
R6204 T14113 T14114 punct (,gift
R6205 T14114 T14111 parataxis gift,goat
R6206 T14115 T14114 prep from,gift
R6207 T14116 T14117 compound L.,Reichardt
R6208 T14117 T14115 pobj Reichardt,from
R6209 T14118 T14117 compound F.,Reichardt
R6210 T14119 T14114 punct ),gift
R6211 T14120 T14111 punct ", ",goat
R6212 T14121 T14111 cc and,goat
R6213 T14122 T14123 compound guinea,pig
R6214 T14123 T14111 conj pig,goat
R6215 T14124 T14123 amod anti-Isl1,pig
R6216 T14125 T14126 punct [,14
R6217 T14126 T14123 parataxis 14,pig
R6218 T14127 T14126 punct ],14
R6219 T14128 T13961 punct .,were
R6220 T14130 T14131 amod Terminal,transferase
R6221 T14131 T14133 npadvmod transferase,mediated
R6222 T14132 T14131 compound deoxynucleotidyl,transferase
R6223 T14133 T14135 amod mediated,labeling
R6224 T14134 T14133 punct -,mediated
R6225 T14135 T14140 nsubjpass labeling,performed
R6226 T14136 T14135 amod biotinylated,labeling
R6227 T14137 T14135 compound UTP,labeling
R6228 T14138 T14135 compound nick,labeling
R6229 T14139 T14135 compound end,labeling
R6230 T14141 T14135 punct (,labeling
R6231 T14142 T14135 appos TUNEL,labeling
R6232 T14143 T14135 punct ),labeling
R6233 T14144 T14145 aux to,detect
R6234 T14145 T14135 advcl detect,labeling
R6235 T14146 T14147 amod apoptotic,cells
R6236 T14147 T14145 dobj cells,detect
R6237 T14148 T14145 prep in,detect
R6238 T14149 T14150 compound E13.5,DRG
R6239 T14150 T14148 pobj DRG,in
R6240 T14151 T14145 prep on,detect
R6241 T14152 T14153 compound cryostat,sections
R6242 T14153 T14151 pobj sections,on
R6243 T14154 T14140 auxpass was,performed
R6244 T14155 T14156 mark as,described
R6245 T14156 T14140 advcl described,performed
R6246 T14157 T14156 agent by,described
R6247 T14158 T14159 det the,manufacturer
R6248 T14159 T14157 pobj manufacturer,by
R6249 T14160 T14161 punct (,Roche
R6250 T14161 T14159 parataxis Roche,manufacturer
R6251 T14162 T14161 punct ", ",Roche
R6252 T14163 T14161 npadvmod Basel,Roche
R6253 T14164 T14161 punct ", ",Roche
R6254 T14165 T14161 npadvmod Switzerland,Roche
R6255 T14166 T14161 punct ),Roche
R6256 T14167 T14140 punct .,performed
R6257 T14169 T14170 amod Quantitative,analysis
R6258 T14170 T14171 nsubjpass analysis,performed
R6259 T14172 T14170 prep of,analysis
R6260 T14173 T14174 nmod TUNEL,cells
R6261 T14174 T14172 pobj cells,of
R6262 T14175 T14173 punct +,TUNEL
R6263 T14176 T14174 compound DRG,cells
R6264 T14177 T14171 auxpass was,performed
R6265 T14178 T14179 advmod essentially,described
R6266 T14179 T14171 advcl described,performed
R6267 T14180 T14179 mark as,described
R6268 T14181 T14182 punct [,27
R6269 T14182 T14179 parataxis 27,described
R6270 T14183 T14182 punct ],27
R6271 T14184 T14171 punct .,performed
R6272 T14186 T14187 compound BrdU,experiments
R6273 T14187 T14191 nsubjpass experiments,performed
R6274 T14188 T14189 compound pulse,chase
R6275 T14189 T14187 compound chase,experiments
R6276 T14190 T14189 punct -,chase
R6277 T14192 T14187 cc and,experiments
R6278 T14193 T14194 compound LacZ,stainings
R6279 T14194 T14187 conj stainings,experiments
R6280 T14195 T14194 compound wholemount,stainings
R6281 T14196 T14191 auxpass were,performed
R6282 T14197 T14198 mark as,described
R6283 T14198 T14191 advcl described,performed
R6284 T14199 T14198 advmod previously,described
R6285 T14200 T14201 punct [,46
R6286 T14201 T14198 parataxis 46,described
R6287 T14202 T14201 punct ],46
R6288 T14203 T14191 punct .,performed
R6289 T14205 T14206 mark For,visualize
R6290 T14206 T14211 advcl visualize,injected
R6291 T14207 T14208 amod anterograde,experiments
R6292 T14208 T14206 nsubj experiments,visualize
R6293 T14209 T14208 compound tracing,experiments
R6294 T14210 T14206 aux to,visualize
R6295 T14212 T14206 dobj projections,visualize
R6296 T14213 T14212 prep of,projections
R6297 T14214 T14215 amod sensory,neurons
R6298 T14215 T14213 pobj neurons,of
R6299 T14216 T14211 punct ", ",injected
R6300 T14217 T14218 npadvmod rhodamine,conjugated
R6301 T14218 T14220 amod conjugated,dextran
R6302 T14219 T14218 punct -,conjugated
R6303 T14220 T14211 nsubjpass dextran,injected
R6304 T14221 T14222 punct (,Probes
R6305 T14222 T14220 parataxis Probes,dextran
R6306 T14223 T14222 compound Molecular,Probes
R6307 T14224 T14222 punct ),Probes
R6308 T14225 T14211 auxpass was,injected
R6309 T14226 T14211 prep into,injected
R6310 T14227 T14228 amod single,DRG
R6311 T14228 T14226 pobj DRG,into
R6312 T14229 T14228 nmod lumbar,DRG
R6313 T14230 T14231 punct (,L3
R6314 T14231 T14228 parataxis L3,DRG
R6315 T14232 T14231 punct ),L3
R6316 T14233 T14211 prep at,injected
R6317 T14234 T14233 pobj E13.5,at
R6318 T14235 T14211 cc or,injected
R6319 T14236 T14211 conj applied,injected
R6320 T14237 T14236 prep to,applied
R6321 T14238 T14239 amod whole,roots
R6322 T14239 T14237 pobj roots,to
R6323 T14240 T14239 amod lumbar,roots
R6324 T14241 T14239 amod dorsal,roots
R6325 T14242 T14243 punct (,L3
R6326 T14243 T14239 parataxis L3,roots
R6327 T14244 T14243 punct ),L3
R6328 T14245 T14236 prep at,applied
R6329 T14246 T14247 amod postnatal,day
R6330 T14247 T14245 pobj day,at
R6331 T14248 T14247 punct (,day
R6332 T14249 T14247 appos P,day
R6333 T14250 T14247 punct ),day
R6334 T14251 T14247 nummod 5,day
R6335 T14252 T14236 advcl using,applied
R6336 T14253 T14254 compound glass,capillaries
R6337 T14254 T14252 dobj capillaries,using
R6338 T14255 T14211 punct .,injected
R6339 T14257 T14258 prep After,incubated
R6340 T14259 T14257 pobj injection,After
R6341 T14260 T14258 punct ", ",incubated
R6342 T14261 T14258 nsubjpass animals,incubated
R6343 T14262 T14258 auxpass were,incubated
R6344 T14263 T14258 prep for,incubated
R6345 T14264 T14265 quantmod 2,3
R6346 T14265 T14267 nummod 3,h
R6347 T14266 T14265 punct –,3
R6348 T14267 T14263 pobj h,for
R6349 T14268 T14269 punct (,E13.5
R6350 T14269 T14267 parataxis E13.5,h
R6351 T14270 T14269 punct ),E13.5
R6352 T14271 T14263 cc or,for
R6353 T14272 T14263 conj overnight,for
R6354 T14273 T14274 punct (,P5
R6355 T14274 T14272 parataxis P5,overnight
R6356 T14275 T14274 punct ),P5
R6357 T14276 T14258 punct .,incubated
R6358 T14278 T14279 compound Cryostat,sections
R6359 T14279 T14280 nsubjpass sections,processed
R6360 T14281 T14280 auxpass were,processed
R6361 T14282 T14280 prep for,processed
R6362 T14283 T14282 pobj immunohistochemistry,for
R6363 T14284 T14285 mark as,described
R6364 T14285 T14280 advcl described,processed
R6365 T14286 T14287 punct [,14
R6366 T14287 T14285 parataxis 14,described
R6367 T14288 T14287 punct ],14
R6368 T14289 T14280 advcl using,processed
R6369 T14290 T14291 npadvmod fluorophore,conjugated
R6370 T14291 T14293 amod conjugated,antibodies
R6371 T14292 T14291 punct -,conjugated
R6372 T14293 T14289 dobj antibodies,using
R6373 T14294 T14293 amod secondary,antibodies
R6374 T14295 T14296 punct (,Probes
R6375 T14296 T14293 parataxis Probes,antibodies
R6376 T14297 T14296 dep 1,Probes
R6377 T14298 T14299 punct :,"1,000"
R6378 T14299 T14297 prep "1,000",1
R6379 T14300 T14296 punct ", ",Probes
R6380 T14301 T14296 compound Molecular,Probes
R6381 T14302 T14296 punct ),Probes
R6382 T14303 T14280 punct .,processed
R6383 T14305 T14306 nsubjpass Images,collected
R6384 T14307 T14306 auxpass were,collected
R6385 T14308 T14306 prep on,collected
R6386 T14309 T14310 det an,microscope
R6387 T14310 T14308 pobj microscope,on
R6388 T14311 T14310 nmod Olympus,microscope
R6389 T14312 T14313 punct (,Tokyo
R6390 T14313 T14311 parataxis Tokyo,Olympus
R6391 T14314 T14313 punct ", ",Tokyo
R6392 T14315 T14313 npadvmod Japan,Tokyo
R6393 T14316 T14313 punct ),Tokyo
R6394 T14317 T14310 amod confocal,microscope
R6395 T14318 T14306 punct .,collected
R6396 T14320 T14321 nsubjpass Images,collected
R6397 T14322 T14320 prep from,Images
R6398 T14323 T14324 nmod in,situ
R6399 T14324 T14325 nmod situ,experiments
R6400 T14325 T14322 pobj experiments,from
R6401 T14326 T14325 compound hybridization,experiments
R6402 T14327 T14321 auxpass were,collected
R6403 T14328 T14321 prep with,collected
R6404 T14329 T14330 det an,camera
R6405 T14330 T14328 pobj camera,with
R6406 T14331 T14332 compound RT,SPOT
R6407 T14332 T14330 compound SPOT,camera
R6408 T14333 T14332 punct -,SPOT
R6409 T14334 T14335 punct (,Instruments
R6410 T14335 T14330 parataxis Instruments,camera
R6411 T14336 T14335 amod Diagnostic,Instruments
R6412 T14337 T14335 punct ", ",Instruments
R6413 T14338 T14339 compound Sterling,Heights
R6414 T14339 T14335 npadvmod Heights,Instruments
R6415 T14340 T14335 punct ", ",Instruments
R6416 T14341 T14335 npadvmod Michigan,Instruments
R6417 T14342 T14335 punct ", ",Instruments
R6418 T14343 T14344 compound United,States
R6419 T14344 T14335 npadvmod States,Instruments
R6420 T14345 T14335 punct ),Instruments
R6421 T14346 T14321 punct ", ",collected
R6422 T14347 T14321 cc and,collected
R6423 T14348 T14349 nmod Corel,Paint
R6424 T14349 T14360 nsubjpass Paint,used
R6425 T14350 T14351 punct (,Prairie
R6426 T14351 T14348 parataxis Prairie,Corel
R6427 T14352 T14351 compound Eden,Prairie
R6428 T14353 T14351 punct ", ",Prairie
R6429 T14354 T14351 npadvmod Minnesota,Prairie
R6430 T14355 T14351 punct ", ",Prairie
R6431 T14356 T14357 compound United,States
R6432 T14357 T14351 npadvmod States,Prairie
R6433 T14358 T14351 punct ),Prairie
R6434 T14359 T14349 compound Photo,Paint
R6435 T14360 T14321 conj used,collected
R6436 T14361 T14349 nummod 10.0,Paint
R6437 T14362 T14360 auxpass was,used
R6438 T14363 T14360 prep for,used
R6439 T14364 T14365 amod digital,processing
R6440 T14365 T14363 pobj processing,for
R6441 T14366 T14365 prep of,processing
R6442 T14367 T14366 pobj images,of
R6443 T14368 T14360 punct .,used
R6444 T14469 T14470 advmod In,vitro
R6445 T14470 T14471 amod vitro,cultures
R6446 T14472 T14471 prep of,cultures
R6447 T14473 T14472 pobj DRG,of
R6448 T14475 T14476 amod Individual,DRG
R6449 T14476 T14478 nsubjpass DRG,dissected
R6450 T14477 T14476 compound lumbar,DRG
R6451 T14479 T14478 auxpass were,dissected
R6452 T14480 T14478 prep from,dissected
R6453 T14481 T14482 nmod E13.5,embryos
R6454 T14482 T14480 pobj embryos,from
R6455 T14483 T14481 cc or,E13.5
R6456 T14484 T14481 conj E14.5,E13.5
R6457 T14485 T14478 cc and,dissected
R6458 T14486 T14478 conj placed,dissected
R6459 T14487 T14486 prep on,placed
R6460 T14488 T14489 npadvmod Matrigel,coated
R6461 T14489 T14502 amod coated,coverslips
R6462 T14490 T14491 punct (,Biosciences
R6463 T14491 T14488 parataxis Biosciences,Matrigel
R6464 T14492 T14491 compound BD,Biosciences
R6465 T14493 T14491 punct ", ",Biosciences
R6466 T14494 T14495 compound San,Jose
R6467 T14495 T14491 npadvmod Jose,Biosciences
R6468 T14496 T14491 punct ", ",Biosciences
R6469 T14497 T14491 npadvmod California,Biosciences
R6470 T14498 T14491 punct ", ",Biosciences
R6471 T14499 T14500 compound United,States
R6472 T14500 T14491 npadvmod States,Biosciences
R6473 T14501 T14491 punct ),Biosciences
R6474 T14502 T14487 pobj coverslips,on
R6475 T14503 T14502 prep in,coverslips
R6476 T14504 T14505 compound DMEM,F12
R6477 T14505 T14503 pobj F12,in
R6478 T14506 T14505 punct /,F12
R6479 T14507 T14508 punct (,Gibco
R6480 T14508 T14505 parataxis Gibco,F12
R6481 T14509 T14508 punct ", ",Gibco
R6482 T14510 T14511 compound San,Diego
R6483 T14511 T14508 npadvmod Diego,Gibco
R6484 T14512 T14508 punct ", ",Gibco
R6485 T14513 T14508 npadvmod California,Gibco
R6486 T14514 T14508 punct ", ",Gibco
R6487 T14515 T14516 compound United,States
R6488 T14516 T14508 npadvmod States,Gibco
R6489 T14517 T14508 punct ),Gibco
R6490 T14518 T14502 punct ", ",coverslips
R6491 T14519 T14520 nummod 2,mM
R6492 T14520 T14521 compound mM,Gln
R6493 T14521 T14502 conj Gln,coverslips
R6494 T14522 T14521 compound L,Gln
R6495 T14523 T14521 punct -,Gln
R6496 T14524 T14525 punct (,Gibco
R6497 T14525 T14521 parataxis Gibco,Gln
R6498 T14526 T14525 punct ),Gibco
R6499 T14527 T14521 punct ", ",Gln
R6500 T14528 T14521 conj N2,Gln
R6501 T14529 T14530 punct (,Gibco
R6502 T14530 T14528 parataxis Gibco,N2
R6503 T14531 T14530 punct ),Gibco
R6504 T14532 T14528 punct ", ",N2
R6505 T14533 T14528 cc and,N2
R6506 T14534 T14535 nummod 1,mg
R6507 T14535 T14536 nmod mg,BSA
R6508 T14536 T14528 conj BSA,N2
R6509 T14537 T14538 punct /,ml
R6510 T14538 T14535 prep ml,mg
R6511 T14539 T14540 punct (,Sigma
R6512 T14540 T14536 parataxis Sigma,BSA
R6513 T14541 T14540 punct ", ",Sigma
R6514 T14542 T14543 compound St.,Louis
R6515 T14543 T14540 npadvmod Louis,Sigma
R6516 T14544 T14540 punct ", ",Sigma
R6517 T14545 T14540 npadvmod Missouri,Sigma
R6518 T14546 T14540 punct ", ",Sigma
R6519 T14547 T14548 compound United,States
R6520 T14548 T14540 npadvmod States,Sigma
R6521 T14549 T14540 punct ),Sigma
R6522 T14550 T14536 prep without,BSA
R6523 T14551 T14550 pobj neurotrophins,without
R6524 T14552 T14478 punct ", ",dissected
R6525 T14553 T14478 cc or,dissected
R6526 T14554 T14478 conj supplemented,dissected
R6527 T14555 T14554 prep with,supplemented
R6528 T14556 T14557 preconj either,NGF
R6529 T14557 T14555 pobj NGF,with
R6530 T14558 T14559 punct (,Gibco
R6531 T14559 T14557 parataxis Gibco,NGF
R6532 T14560 T14561 nummod 100,ng
R6533 T14561 T14559 dep ng,Gibco
R6534 T14562 T14563 punct /,ml
R6535 T14563 T14561 prep ml,ng
R6536 T14564 T14559 punct ", ",Gibco
R6537 T14565 T14559 punct ),Gibco
R6538 T14566 T14557 cc or,NGF
R6539 T14567 T14557 conj NT,NGF
R6540 T14568 T14567 punct -,NT
R6541 T14569 T14567 nummod 3,NT
R6542 T14570 T14571 punct (,Sigma
R6543 T14571 T14567 parataxis Sigma,NT
R6544 T14572 T14573 nummod 20,ng
R6545 T14573 T14571 dep ng,Sigma
R6546 T14574 T14575 punct /,ml
R6547 T14575 T14573 prep ml,ng
R6548 T14576 T14571 punct ", ",Sigma
R6549 T14577 T14571 punct ),Sigma
R6550 T14578 T14478 punct .,dissected
R6551 T14580 T14581 compound DRG,explants
R6552 T14581 T14582 nsubjpass explants,cultured
R6553 T14583 T14584 punct (,20
R6554 T14584 T14581 parataxis 20,explants
R6555 T14585 T14584 nsubj n,20
R6556 T14586 T14584 punct ≥,20
R6557 T14587 T14584 prep for,20
R6558 T14588 T14589 det each,condition
R6559 T14589 T14587 pobj condition,for
R6560 T14590 T14584 punct ),20
R6561 T14591 T14582 auxpass were,cultured
R6562 T14592 T14582 prep for,cultured
R6563 T14593 T14594 nummod 48,h
R6564 T14594 T14592 pobj h,for
R6565 T14595 T14582 punct ", ",cultured
R6566 T14596 T14582 conj processed,cultured
R6567 T14597 T14596 prep for,processed
R6568 T14598 T14597 pobj immunocytochemistry,for
R6569 T14599 T14596 punct ", ",processed
R6570 T14600 T14596 cc and,processed
R6571 T14601 T14596 conj analyzed,processed
R6572 T14602 T14601 advcl using,analyzed
R6573 T14603 T14604 amod confocal,microscopy
R6574 T14604 T14602 dobj microscopy,using
R6575 T14605 T14582 punct .,cultured
R6577 T14754 T14755 compound Western,blot
R6578 T14755 T14756 compound blot,analysis
R6579 T14758 T14759 compound Lumbar,DRG
R6580 T14759 T14760 nsubjpass DRG,isolated
R6581 T14761 T14759 prep from,DRG
R6582 T14762 T14763 compound E16.5,embryos
R6583 T14763 T14761 pobj embryos,from
R6584 T14764 T14760 auxpass were,isolated
R6585 T14765 T14760 punct ", ",isolated
R6586 T14766 T14767 advmod mechanically,disrupted
R6587 T14767 T14760 conj disrupted,isolated
R6588 T14768 T14767 punct ", ",disrupted
R6589 T14769 T14767 conj homogenized,disrupted
R6590 T14770 T14769 advcl using,homogenized
R6591 T14771 T14772 compound glass,beads
R6592 T14772 T14770 dobj beads,using
R6593 T14773 T14774 punct (,Sigma
R6594 T14774 T14772 parataxis Sigma,beads
R6595 T14775 T14774 punct ),Sigma
R6596 T14776 T14769 punct ", ",homogenized
R6597 T14777 T14769 cc and,homogenized
R6598 T14778 T14769 conj lysed,homogenized
R6599 T14779 T14778 prep in,lysed
R6600 T14780 T14781 amod standard,buffer
R6601 T14781 T14779 pobj buffer,in
R6602 T14782 T14781 compound lysis,buffer
R6603 T14783 T14781 acl supplemented,buffer
R6604 T14784 T14783 prep with,supplemented
R6605 T14785 T14786 nmod protease,inhibitors
R6606 T14786 T14784 pobj inhibitors,with
R6607 T14787 T14785 cc and,protease
R6608 T14788 T14785 conj phosphatase,protease
R6609 T14789 T14790 mark as,described
R6610 T14790 T14783 advcl described,supplemented
R6611 T14791 T14792 punct [,47
R6612 T14792 T14790 parataxis 47,described
R6613 T14793 T14792 punct ],47
R6614 T14794 T14760 punct .,isolated
R6615 T14796 T14797 compound Protein,extracts
R6616 T14797 T14798 nsubjpass extracts,resolved
R6617 T14799 T14798 auxpass were,resolved
R6618 T14800 T14798 agent by,resolved
R6619 T14801 T14802 compound SDS,PAGE
R6620 T14802 T14800 pobj PAGE,by
R6621 T14803 T14802 punct -,PAGE
R6622 T14804 T14798 punct ", ",resolved
R6623 T14805 T14798 cc and,resolved
R6624 T14806 T14807 nsubjpass immunoblotting,performed
R6625 T14807 T14798 conj performed,resolved
R6626 T14808 T14807 auxpass was,performed
R6627 T14809 T14807 advcl using,performed
R6628 T14810 T14809 dobj antibodies,using
R6629 T14811 T14810 prep against,antibodies
R6630 T14812 T14811 pobj Akt,against
R6631 T14813 T14812 punct ", ",Akt
R6632 T14814 T14815 compound p,Akt
R6633 T14815 T14812 conj Akt,Akt
R6634 T14816 T14815 punct -,Akt
R6635 T14817 T14818 punct (,Ser473
R6636 T14818 T14815 parataxis Ser473,Akt
R6637 T14819 T14818 punct ),Ser473
R6638 T14820 T14815 punct ", ",Akt
R6639 T14821 T14815 conj CREB,Akt
R6640 T14822 T14821 punct ", ",CREB
R6641 T14823 T14824 compound p,CREB
R6642 T14824 T14821 conj CREB,CREB
R6643 T14825 T14824 punct -,CREB
R6644 T14826 T14827 punct (,Ser133
R6645 T14827 T14824 parataxis Ser133,CREB
R6646 T14828 T14827 punct ),Ser133
R6647 T14829 T14824 punct ", ",CREB
R6648 T14830 T14824 conj Bax,CREB
R6649 T14831 T14830 punct ", ",Bax
R6650 T14832 T14833 compound Bcl,xl
R6651 T14833 T14830 conj xl,Bax
R6652 T14834 T14833 punct -,xl
R6653 T14835 T14836 punct (,Technology
R6654 T14836 T14833 parataxis Technology,xl
R6655 T14837 T14836 compound Cell,Technology
R6656 T14838 T14836 compound Signaling,Technology
R6657 T14839 T14836 punct ", ",Technology
R6658 T14840 T14836 npadvmod Beverly,Technology
R6659 T14841 T14836 punct ", ",Technology
R6660 T14842 T14836 npadvmod Massachusetts,Technology
R6661 T14843 T14836 punct ", ",Technology
R6662 T14844 T14845 compound United,States
R6663 T14845 T14836 npadvmod States,Technology
R6664 T14846 T14836 punct ),Technology
R6665 T14847 T14833 punct ", ",xl
R6666 T14848 T14833 cc and,xl
R6667 T14849 T14833 conj Bcl2,xl
R6668 T14850 T14851 punct (,Pharmingen
R6669 T14851 T14849 parataxis Pharmingen,Bcl2
R6670 T14852 T14851 compound BD,Pharmingen
R6671 T14853 T14851 punct ", ",Pharmingen
R6672 T14854 T14855 compound San,Diego
R6673 T14855 T14851 npadvmod Diego,Pharmingen
R6674 T14856 T14851 punct ", ",Pharmingen
R6675 T14857 T14851 npadvmod California,Pharmingen
R6676 T14858 T14851 punct ", ",Pharmingen
R6677 T14859 T14860 compound United,States
R6678 T14860 T14851 npadvmod States,Pharmingen
R6679 T14861 T14851 punct ),Pharmingen
R6680 T14862 T14807 punct .,performed
R6681 T14864 T14865 prep For,scanned
R6682 T14866 T14864 pobj quantification,For
R6683 T14867 T14865 punct ", ",scanned
R6684 T14868 T14865 nsubjpass films,scanned
R6685 T14869 T14870 punct (,AR
R6686 T14870 T14868 parataxis AR,films
R6687 T14871 T14872 compound X,OMAT
R6688 T14872 T14870 compound OMAT,AR
R6689 T14873 T14872 punct -,OMAT
R6690 T14874 T14870 punct ", ",AR
R6691 T14875 T14876 compound Eastman,Kodak
R6692 T14876 T14870 npadvmod Kodak,AR
R6693 T14877 T14870 punct ", ",AR
R6694 T14878 T14870 npadvmod Rochester,AR
R6695 T14879 T14870 punct ", ",AR
R6696 T14880 T14881 compound New,York
R6697 T14881 T14870 npadvmod York,AR
R6698 T14882 T14870 punct ", ",AR
R6699 T14883 T14884 compound United,States
R6700 T14884 T14870 npadvmod States,AR
R6701 T14885 T14870 punct ),AR
R6702 T14886 T14865 auxpass were,scanned
R6703 T14887 T14865 cc and,scanned
R6704 T14888 T14889 nsubjpass densitometry,performed
R6705 T14889 T14865 conj performed,scanned
R6706 T14890 T14889 auxpass was,performed
R6707 T14891 T14889 advcl using,performed
R6708 T14892 T14891 dobj IMAGEQUANT,using
R6709 T14893 T14892 nummod 5.2,IMAGEQUANT
R6710 T14894 T14895 punct (,Dynamics
R6711 T14895 T14892 parataxis Dynamics,IMAGEQUANT
R6712 T14896 T14895 compound Molecular,Dynamics
R6713 T14897 T14895 punct ", ",Dynamics
R6714 T14898 T14895 npadvmod Amersham,Dynamics
R6715 T14899 T14895 punct ", ",Dynamics
R6716 T14900 T14895 npadvmod Uppsala,Dynamics
R6717 T14901 T14895 punct ", ",Dynamics
R6718 T14902 T14895 npadvmod Sweden,Dynamics
R6719 T14903 T14895 punct ),Dynamics
R6720 T14904 T14889 punct .,performed
R6721 T15022 T15023 amod Electrophysiological,analysis
R6722 T15023 T15024 nsubjpass analysis,performed
R6723 T15025 T15024 auxpass was,performed
R6724 T15026 T15027 mark as,described
R6725 T15027 T15024 advcl described,performed
R6726 T15028 T15027 advmod previously,described
R6727 T15029 T15030 punct [,48
R6728 T15030 T15027 parataxis 48,described
R6729 T15031 T15030 punct ],48
R6730 T15032 T15024 punct .,performed
R6731 T15034 T15035 advmod Briefly,made
R6732 T15036 T15035 punct ", ",made
R6733 T15037 T15038 amod intracellular,recordings
R6734 T15038 T15035 nsubjpass recordings,made
R6735 T15039 T15038 prep from,recordings
R6736 T15040 T15041 amod identified,neurons
R6737 T15041 T15039 pobj neurons,from
R6738 T15042 T15041 compound quadriceps,neurons
R6739 T15043 T15041 compound motor,neurons
R6740 T15044 T15035 auxpass were,made
R6741 T15045 T15035 advcl using,made
R6742 T15046 T15047 amod sharp,electrodes
R6743 T15047 T15045 dobj electrodes,using
R6744 T15048 T15049 punct (,MΩ
R6745 T15049 T15045 parataxis MΩ,using
R6746 T15050 T15051 quantmod 75,120
R6747 T15051 T15049 nummod 120,MΩ
R6748 T15052 T15051 punct –,120
R6749 T15053 T15049 punct ", ",MΩ
R6750 T15054 T15055 compound 3M,KCl
R6751 T15055 T15049 appos KCl,MΩ
R6752 T15056 T15049 punct ),MΩ
R6753 T15057 T15035 punct .,made
R6754 T15059 T15060 amod Average,responses
R6755 T15060 T15061 nsubjpass responses,acquired
R6756 T15062 T15063 punct (,trials
R6757 T15063 T15060 parataxis trials,responses
R6758 T15064 T15065 quantmod 10,20
R6759 T15065 T15063 nummod 20,trials
R6760 T15066 T15065 punct –,20
R6761 T15067 T15063 punct ),trials
R6762 T15068 T15060 prep from,responses
R6763 T15069 T15070 compound suprathreshold,stimulation
R6764 T15070 T15068 pobj stimulation,from
R6765 T15071 T15070 compound nerve,stimulation
R6766 T15072 T15073 punct (,strength
R6767 T15073 T15070 parataxis strength,stimulation
R6768 T15074 T15073 nummod 1.5,strength
R6769 T15075 T15074 quantmod times,1.5
R6770 T15076 T15073 det the,strength
R6771 T15077 T15078 dep that,evokes
R6772 T15078 T15073 relcl evokes,strength
R6773 T15079 T15080 amod maximal,response
R6774 T15080 T15078 dobj response,evokes
R6775 T15081 T15080 amod monosynaptic,response
R6776 T15082 T15073 punct ),strength
R6777 T15083 T15070 prep of,stimulation
R6778 T15084 T15085 det the,nerve
R6779 T15085 T15083 pobj nerve,of
R6780 T15086 T15085 compound quadriceps,nerve
R6781 T15087 T15061 auxpass were,acquired
R6782 T15088 T15061 prep with,acquired
R6783 T15089 T15090 compound LTP,software
R6784 T15090 T15088 pobj software,with
R6785 T15091 T15092 punct [,49
R6786 T15092 T15061 parataxis 49,acquired
R6787 T15093 T15092 punct ],49
R6788 T15094 T15061 punct .,acquired
R6789 T15096 T15097 advmod Only,cells
R6790 T15097 T15098 nsubjpass cells,considered
R6791 T15099 T15097 prep with,cells
R6792 T15100 T15101 amod stable,potentials
R6793 T15101 T15099 pobj potentials,with
R6794 T15102 T15101 compound resting,potentials
R6795 T15103 T15104 advmod more,negative
R6796 T15104 T15101 amod negative,potentials
R6797 T15105 T15104 prep than,negative
R6798 T15106 T15107 punct −,50
R6799 T15107 T15108 nummod 50,mV
R6800 T15108 T15105 pobj mV,than
R6801 T15109 T15098 auxpass were,considered
R6802 T15110 T15098 prep for,considered
R6803 T15111 T15110 pobj analysis,for
R6804 T15112 T15098 punct .,considered
R6805 T15114 T15115 amod Monosynaptic,amplitudes
R6806 T15115 T15116 nsubjpass amplitudes,determined
R6807 T15117 T15116 auxpass were,determined
R6808 T15118 T15116 advmod offline,determined
R6809 T15119 T15116 advcl using,determined
R6810 T15120 T15121 compound custom,routines
R6811 T15121 T15119 dobj routines,using
R6812 T15122 T15119 prep in,using
R6813 T15123 T15124 det the,environment
R6814 T15124 T15122 pobj environment,in
R6815 T15125 T15124 compound Matlab,environment
R6816 T15126 T15127 punct (,Mathworks
R6817 T15127 T15124 parataxis Mathworks,environment
R6818 T15128 T15127 det The,Mathworks
R6819 T15129 T15127 punct ", ",Mathworks
R6820 T15130 T15127 npadvmod Natick,Mathworks
R6821 T15131 T15127 punct ", ",Mathworks
R6822 T15132 T15127 npadvmod Massachusetts,Mathworks
R6823 T15133 T15127 punct ", ",Mathworks
R6824 T15134 T15135 compound United,States
R6825 T15135 T15127 npadvmod States,Mathworks
R6826 T15136 T15127 punct ),Mathworks
R6827 T15137 T15138 mark as,described
R6828 T15138 T15119 advcl described,using
R6829 T15139 T15138 advmod previously,described
R6830 T15140 T15141 punct [,48
R6831 T15141 T15138 parataxis 48,described
R6832 T15142 T15141 punct ],48
R6833 T15143 T15116 punct .,determined