Id |
Subject |
Object |
Predicate |
Lexical cue |
T6663 |
0-10 |
JJ |
denotes |
Precocious |
T6665 |
11-14 |
NN |
denotes |
EWS |
T6667 |
14-15 |
HYPH |
denotes |
- |
T6666 |
15-19 |
NN |
denotes |
Pea3 |
T6664 |
20-30 |
NN |
denotes |
Expression |
T6668 |
31-39 |
VBZ |
denotes |
Promotes |
T6669 |
40-52 |
NN |
denotes |
Neurotrophin |
T6671 |
52-53 |
HYPH |
denotes |
- |
T6670 |
53-64 |
JJ |
denotes |
Independent |
T6672 |
65-73 |
NN |
denotes |
Survival |
T6673 |
74-77 |
CC |
denotes |
and |
T6674 |
78-85 |
NN |
denotes |
Neurite |
T6675 |
86-95 |
NN |
denotes |
Outgrowth |
T6676 |
95-293 |
sentence |
denotes |
To begin to address the cellular and molecular mechanisms involved in the distinct biological actions of EWS-Pea3 at different developmental stages, we first turned to in vitro culture experiments. |
T6677 |
96-98 |
TO |
denotes |
To |
T6678 |
99-104 |
VB |
denotes |
begin |
T6680 |
105-107 |
TO |
denotes |
to |
T6681 |
108-115 |
VB |
denotes |
address |
T6682 |
116-119 |
DT |
denotes |
the |
T6684 |
120-128 |
JJ |
denotes |
cellular |
T6685 |
129-132 |
CC |
denotes |
and |
T6686 |
133-142 |
JJ |
denotes |
molecular |
T6683 |
143-153 |
NNS |
denotes |
mechanisms |
T6687 |
154-162 |
VBN |
denotes |
involved |
T6688 |
163-165 |
IN |
denotes |
in |
T6689 |
166-169 |
DT |
denotes |
the |
T6691 |
170-178 |
JJ |
denotes |
distinct |
T6692 |
179-189 |
JJ |
denotes |
biological |
T6690 |
190-197 |
NNS |
denotes |
actions |
T6693 |
198-200 |
IN |
denotes |
of |
T6694 |
201-204 |
NN |
denotes |
EWS |
T6696 |
204-205 |
HYPH |
denotes |
- |
T6695 |
205-209 |
NN |
denotes |
Pea3 |
T6697 |
210-212 |
IN |
denotes |
at |
T6698 |
213-222 |
JJ |
denotes |
different |
T6700 |
223-236 |
JJ |
denotes |
developmental |
T6699 |
237-243 |
NNS |
denotes |
stages |
T6701 |
243-245 |
, |
denotes |
, |
T6702 |
245-247 |
PRP |
denotes |
we |
T6703 |
248-253 |
RB |
denotes |
first |
T6679 |
254-260 |
VBD |
denotes |
turned |
T6704 |
261-263 |
IN |
denotes |
to |
T6705 |
264-266 |
FW |
denotes |
in |
T6706 |
267-272 |
FW |
denotes |
vitro |
T6708 |
273-280 |
NN |
denotes |
culture |
T6707 |
281-292 |
NNS |
denotes |
experiments |
T6709 |
292-293 |
. |
denotes |
. |
T6710 |
293-560 |
sentence |
denotes |
These experiments permit assessment of whether precocious ETS transcription factor signaling influences neuronal survival and in vitro neurite outgrowth of DRG neurons, two parameters prominently influenced by target-derived neurotrophic factors and their receptors. |
T6711 |
294-299 |
DT |
denotes |
These |
T6712 |
300-311 |
NNS |
denotes |
experiments |
T6713 |
312-318 |
VBP |
denotes |
permit |
T6714 |
319-329 |
NN |
denotes |
assessment |
T6715 |
330-332 |
IN |
denotes |
of |
T6716 |
333-340 |
IN |
denotes |
whether |
T6718 |
341-351 |
JJ |
denotes |
precocious |
T6720 |
352-355 |
NN |
denotes |
ETS |
T6721 |
356-369 |
NN |
denotes |
transcription |
T6722 |
370-376 |
NN |
denotes |
factor |
T6719 |
377-386 |
NN |
denotes |
signaling |
T6717 |
387-397 |
VBZ |
denotes |
influences |
T6723 |
398-406 |
JJ |
denotes |
neuronal |
T6724 |
407-415 |
NN |
denotes |
survival |
T6725 |
416-419 |
CC |
denotes |
and |
T6726 |
420-422 |
FW |
denotes |
in |
T6727 |
423-428 |
FW |
denotes |
vitro |
T6729 |
429-436 |
NN |
denotes |
neurite |
T6728 |
437-446 |
NN |
denotes |
outgrowth |
T6730 |
447-449 |
IN |
denotes |
of |
T6731 |
450-453 |
NN |
denotes |
DRG |
T6732 |
454-461 |
NNS |
denotes |
neurons |
T6733 |
461-463 |
, |
denotes |
, |
T6734 |
463-466 |
CD |
denotes |
two |
T6735 |
467-477 |
NNS |
denotes |
parameters |
T6736 |
478-489 |
RB |
denotes |
prominently |
T6737 |
490-500 |
VBN |
denotes |
influenced |
T6738 |
501-503 |
IN |
denotes |
by |
T6739 |
504-510 |
NN |
denotes |
target |
T6741 |
510-511 |
HYPH |
denotes |
- |
T6740 |
511-518 |
VBN |
denotes |
derived |
T6743 |
519-531 |
JJ |
denotes |
neurotrophic |
T6742 |
532-539 |
NNS |
denotes |
factors |
T6744 |
540-543 |
CC |
denotes |
and |
T6745 |
544-549 |
PRP$ |
denotes |
their |
T6746 |
550-559 |
NNS |
denotes |
receptors |
T6747 |
559-560 |
. |
denotes |
. |
T6748 |
560-810 |
sentence |
denotes |
We cultured E13.5 whole DRG explants from wild-type and TauEWS-Pea3/+ Isl1Cre/+ embryos in the presence of NGF or NT-3 or in the absence of neurotrophins and analyzed neuronal survival and neurite outgrowth on matrigel substrate after 48 h in vitro. |
T6749 |
561-563 |
PRP |
denotes |
We |
T6750 |
564-572 |
VBD |
denotes |
cultured |
T6751 |
573-578 |
NN |
denotes |
E13.5 |
T6753 |
579-584 |
JJ |
denotes |
whole |
T6754 |
585-588 |
NN |
denotes |
DRG |
T6752 |
589-597 |
NNS |
denotes |
explants |
T6755 |
598-602 |
IN |
denotes |
from |
T6756 |
603-607 |
JJ |
denotes |
wild |
T6758 |
607-608 |
HYPH |
denotes |
- |
T6757 |
608-612 |
NN |
denotes |
type |
T6760 |
613-616 |
CC |
denotes |
and |
T6761 |
617-623 |
NN |
denotes |
TauEWS |
T6763 |
623-624 |
HYPH |
denotes |
- |
T6764 |
624-628 |
NN |
denotes |
Pea3 |
T6765 |
628-629 |
HYPH |
denotes |
/ |
T6766 |
629-630 |
SYM |
denotes |
+ |
T6762 |
631-638 |
NN |
denotes |
Isl1Cre |
T6767 |
638-639 |
HYPH |
denotes |
/ |
T6768 |
639-640 |
SYM |
denotes |
+ |
T6759 |
641-648 |
NNS |
denotes |
embryos |
T6769 |
649-651 |
IN |
denotes |
in |
T6770 |
652-655 |
DT |
denotes |
the |
T6771 |
656-664 |
NN |
denotes |
presence |
T6772 |
665-667 |
IN |
denotes |
of |
T6773 |
668-671 |
NN |
denotes |
NGF |
T6774 |
672-674 |
CC |
denotes |
or |
T6775 |
675-677 |
NN |
denotes |
NT |
T6776 |
677-678 |
HYPH |
denotes |
- |
T6777 |
678-679 |
CD |
denotes |
3 |
T6778 |
680-682 |
CC |
denotes |
or |
T6779 |
683-685 |
IN |
denotes |
in |
T6780 |
686-689 |
DT |
denotes |
the |
T6781 |
690-697 |
NN |
denotes |
absence |
T6782 |
698-700 |
IN |
denotes |
of |
T6783 |
701-714 |
NNS |
denotes |
neurotrophins |
T6784 |
715-718 |
CC |
denotes |
and |
T6785 |
719-727 |
VBN |
denotes |
analyzed |
T6786 |
728-736 |
JJ |
denotes |
neuronal |
T6787 |
737-745 |
NN |
denotes |
survival |
T6788 |
746-749 |
CC |
denotes |
and |
T6789 |
750-757 |
NN |
denotes |
neurite |
T6790 |
758-767 |
NN |
denotes |
outgrowth |
T6791 |
768-770 |
IN |
denotes |
on |
T6792 |
771-779 |
NN |
denotes |
matrigel |
T6793 |
780-789 |
NN |
denotes |
substrate |
T6794 |
790-795 |
IN |
denotes |
after |
T6795 |
796-798 |
CD |
denotes |
48 |
T6796 |
799-800 |
NN |
denotes |
h |
T6797 |
801-803 |
FW |
denotes |
in |
T6798 |
804-809 |
FW |
denotes |
vitro |
T6799 |
809-810 |
. |
denotes |
. |
T6800 |
810-893 |
sentence |
denotes |
Without neurotrophic support, very few wild-type DRG neurons survived (Figure 4A). |
T6801 |
811-818 |
IN |
denotes |
Without |
T6803 |
819-831 |
JJ |
denotes |
neurotrophic |
T6804 |
832-839 |
NN |
denotes |
support |
T6805 |
839-841 |
, |
denotes |
, |
T6806 |
841-845 |
RB |
denotes |
very |
T6807 |
846-849 |
JJ |
denotes |
few |
T6809 |
850-854 |
JJ |
denotes |
wild |
T6811 |
854-855 |
HYPH |
denotes |
- |
T6810 |
855-859 |
NN |
denotes |
type |
T6812 |
860-863 |
NN |
denotes |
DRG |
T6808 |
864-871 |
NNS |
denotes |
neurons |
T6802 |
872-880 |
VBD |
denotes |
survived |
T6813 |
881-882 |
-LRB- |
denotes |
( |
T6815 |
882-888 |
NN |
denotes |
Figure |
T6814 |
889-891 |
NN |
denotes |
4A |
T6816 |
891-892 |
-RRB- |
denotes |
) |
T6817 |
892-893 |
. |
denotes |
. |
T6818 |
893-1004 |
sentence |
denotes |
In contrast, culturing wild-type DRG with neurotrophic factors led to neuronal survival and neurite outgrowth. |
T6819 |
894-896 |
IN |
denotes |
In |
T6821 |
897-905 |
NN |
denotes |
contrast |
T6822 |
905-907 |
, |
denotes |
, |
T6823 |
907-916 |
VBG |
denotes |
culturing |
T6824 |
917-921 |
JJ |
denotes |
wild |
T6826 |
921-922 |
HYPH |
denotes |
- |
T6825 |
922-926 |
NN |
denotes |
type |
T6827 |
927-930 |
NN |
denotes |
DRG |
T6828 |
931-935 |
IN |
denotes |
with |
T6829 |
936-948 |
JJ |
denotes |
neurotrophic |
T6830 |
949-956 |
NNS |
denotes |
factors |
T6820 |
957-960 |
VBD |
denotes |
led |
T6831 |
961-963 |
IN |
denotes |
to |
T6832 |
964-972 |
JJ |
denotes |
neuronal |
T6833 |
973-981 |
NN |
denotes |
survival |
T6834 |
982-985 |
CC |
denotes |
and |
T6835 |
986-993 |
NN |
denotes |
neurite |
T6836 |
994-1003 |
NN |
denotes |
outgrowth |
T6837 |
1003-1004 |
. |
denotes |
. |
T6838 |
1004-1324 |
sentence |
denotes |
Addition of NGF, which supports survival of cutaneous afferents, resulted in straight and unbranched neurite outgrowth (Figure 4B), while cultures grown in the presence of NT-3, which supports survival of proprioceptive afferents, resulted in a highly branched neurite outgrowth pattern after 48 h in vitro (Figure 4C). |
T6839 |
1005-1013 |
NN |
denotes |
Addition |
T6841 |
1014-1016 |
IN |
denotes |
of |
T6842 |
1017-1020 |
NN |
denotes |
NGF |
T6843 |
1020-1022 |
, |
denotes |
, |
T6844 |
1022-1027 |
WDT |
denotes |
which |
T6845 |
1028-1036 |
VBZ |
denotes |
supports |
T6846 |
1037-1045 |
NN |
denotes |
survival |
T6847 |
1046-1048 |
IN |
denotes |
of |
T6848 |
1049-1058 |
JJ |
denotes |
cutaneous |
T6849 |
1059-1068 |
NNS |
denotes |
afferents |
T6850 |
1068-1070 |
, |
denotes |
, |
T6840 |
1070-1078 |
VBD |
denotes |
resulted |
T6851 |
1079-1081 |
IN |
denotes |
in |
T6852 |
1082-1090 |
JJ |
denotes |
straight |
T6854 |
1091-1094 |
CC |
denotes |
and |
T6855 |
1095-1105 |
JJ |
denotes |
unbranched |
T6856 |
1106-1113 |
NN |
denotes |
neurite |
T6853 |
1114-1123 |
NN |
denotes |
outgrowth |
T6857 |
1124-1125 |
-LRB- |
denotes |
( |
T6859 |
1125-1131 |
NN |
denotes |
Figure |
T6858 |
1132-1134 |
NN |
denotes |
4B |
T6860 |
1134-1135 |
-RRB- |
denotes |
) |
T6861 |
1135-1137 |
, |
denotes |
, |
T6862 |
1137-1142 |
IN |
denotes |
while |
T6864 |
1143-1151 |
NNS |
denotes |
cultures |
T6865 |
1152-1157 |
VBN |
denotes |
grown |
T6866 |
1158-1160 |
IN |
denotes |
in |
T6867 |
1161-1164 |
DT |
denotes |
the |
T6868 |
1165-1173 |
NN |
denotes |
presence |
T6869 |
1174-1176 |
IN |
denotes |
of |
T6870 |
1177-1179 |
NN |
denotes |
NT |
T6871 |
1179-1180 |
HYPH |
denotes |
- |
T6872 |
1180-1181 |
CD |
denotes |
3 |
T6873 |
1181-1183 |
, |
denotes |
, |
T6874 |
1183-1188 |
WDT |
denotes |
which |
T6875 |
1189-1197 |
VBZ |
denotes |
supports |
T6876 |
1198-1206 |
NN |
denotes |
survival |
T6877 |
1207-1209 |
IN |
denotes |
of |
T6878 |
1210-1224 |
JJ |
denotes |
proprioceptive |
T6879 |
1225-1234 |
NNS |
denotes |
afferents |
T6880 |
1234-1236 |
, |
denotes |
, |
T6863 |
1236-1244 |
VBD |
denotes |
resulted |
T6881 |
1245-1247 |
IN |
denotes |
in |
T6882 |
1248-1249 |
DT |
denotes |
a |
T6884 |
1250-1256 |
RB |
denotes |
highly |
T6885 |
1257-1265 |
VBN |
denotes |
branched |
T6886 |
1266-1273 |
NN |
denotes |
neurite |
T6887 |
1274-1283 |
NN |
denotes |
outgrowth |
T6883 |
1284-1291 |
NN |
denotes |
pattern |
T6888 |
1292-1297 |
IN |
denotes |
after |
T6889 |
1298-1300 |
CD |
denotes |
48 |
T6890 |
1301-1302 |
NN |
denotes |
h |
T6891 |
1303-1305 |
FW |
denotes |
in |
T6892 |
1306-1311 |
FW |
denotes |
vitro |
T6893 |
1312-1313 |
-LRB- |
denotes |
( |
T6895 |
1313-1319 |
NN |
denotes |
Figure |
T6894 |
1320-1322 |
NN |
denotes |
4C |
T6896 |
1322-1323 |
-RRB- |
denotes |
) |
T6897 |
1323-1324 |
. |
denotes |
. |
T6898 |
1324-1534 |
sentence |
denotes |
Surprisingly, DRG neurons isolated from TauEWS-Pea3/+ Isl1Cre/+ embryos and cultured without neurotrophic support survived after 48 h in vitro and had established long and highly branched neurites (Figure 4D). |
T6899 |
1325-1337 |
RB |
denotes |
Surprisingly |
T6901 |
1337-1339 |
, |
denotes |
, |
T6902 |
1339-1342 |
NN |
denotes |
DRG |
T6903 |
1343-1350 |
NNS |
denotes |
neurons |
T6904 |
1351-1359 |
VBN |
denotes |
isolated |
T6905 |
1360-1364 |
IN |
denotes |
from |
T6906 |
1365-1371 |
NN |
denotes |
TauEWS |
T6908 |
1371-1372 |
HYPH |
denotes |
- |
T6909 |
1372-1376 |
NN |
denotes |
Pea3 |
T6910 |
1376-1377 |
HYPH |
denotes |
/ |
T6911 |
1377-1378 |
SYM |
denotes |
+ |
T6907 |
1379-1386 |
NN |
denotes |
Isl1Cre |
T6913 |
1386-1387 |
HYPH |
denotes |
/ |
T6914 |
1387-1388 |
SYM |
denotes |
+ |
T6912 |
1389-1396 |
NNS |
denotes |
embryos |
T6915 |
1397-1400 |
CC |
denotes |
and |
T6916 |
1401-1409 |
VBN |
denotes |
cultured |
T6917 |
1410-1417 |
IN |
denotes |
without |
T6918 |
1418-1430 |
JJ |
denotes |
neurotrophic |
T6919 |
1431-1438 |
NN |
denotes |
support |
T6900 |
1439-1447 |
VBD |
denotes |
survived |
T6920 |
1448-1453 |
IN |
denotes |
after |
T6921 |
1454-1456 |
CD |
denotes |
48 |
T6922 |
1457-1458 |
NN |
denotes |
h |
T6923 |
1459-1461 |
FW |
denotes |
in |
T6924 |
1462-1467 |
FW |
denotes |
vitro |
T6925 |
1468-1471 |
CC |
denotes |
and |
T6926 |
1472-1475 |
VBD |
denotes |
had |
T6927 |
1476-1487 |
VBN |
denotes |
established |
T6928 |
1488-1492 |
JJ |
denotes |
long |
T6930 |
1493-1496 |
CC |
denotes |
and |
T6931 |
1497-1503 |
RB |
denotes |
highly |
T6932 |
1504-1512 |
VBN |
denotes |
branched |
T6929 |
1513-1521 |
NNS |
denotes |
neurites |
T6933 |
1522-1523 |
-LRB- |
denotes |
( |
T6935 |
1523-1529 |
NN |
denotes |
Figure |
T6934 |
1530-1532 |
NN |
denotes |
4D |
T6936 |
1532-1533 |
-RRB- |
denotes |
) |
T6937 |
1533-1534 |
. |
denotes |
. |
T6938 |
1534-1679 |
sentence |
denotes |
Neither the pattern of neurite outgrowth nor neuronal survival changed significantly after application of either NGF or NT-3 (Figure 4E and 4F). |
T6939 |
1535-1542 |
CC |
denotes |
Neither |
T6941 |
1543-1546 |
DT |
denotes |
the |
T6940 |
1547-1554 |
NN |
denotes |
pattern |
T6943 |
1555-1557 |
IN |
denotes |
of |
T6944 |
1558-1565 |
NN |
denotes |
neurite |
T6945 |
1566-1575 |
NN |
denotes |
outgrowth |
T6946 |
1576-1579 |
CC |
denotes |
nor |
T6947 |
1580-1588 |
JJ |
denotes |
neuronal |
T6948 |
1589-1597 |
NN |
denotes |
survival |
T6942 |
1598-1605 |
VBD |
denotes |
changed |
T6949 |
1606-1619 |
RB |
denotes |
significantly |
T6950 |
1620-1625 |
IN |
denotes |
after |
T6951 |
1626-1637 |
NN |
denotes |
application |
T6952 |
1638-1640 |
IN |
denotes |
of |
T6953 |
1641-1647 |
CC |
denotes |
either |
T6954 |
1648-1651 |
NN |
denotes |
NGF |
T6955 |
1652-1654 |
CC |
denotes |
or |
T6956 |
1655-1657 |
NN |
denotes |
NT |
T6957 |
1657-1658 |
HYPH |
denotes |
- |
T6958 |
1658-1659 |
CD |
denotes |
3 |
T6959 |
1660-1661 |
-LRB- |
denotes |
( |
T6961 |
1661-1667 |
NN |
denotes |
Figure |
T6960 |
1668-1670 |
NN |
denotes |
4E |
T6962 |
1671-1674 |
CC |
denotes |
and |
T6963 |
1675-1677 |
NN |
denotes |
4F |
T6964 |
1677-1678 |
-RRB- |
denotes |
) |
T6965 |
1678-1679 |
. |
denotes |
. |
T6966 |
1679-2372 |
sentence |
denotes |
Figure 4 Neurotrophin-Independent Neurite Outgrowth In Vitro of DRG Neurons Expressing EWS-Pea3 Precociously
E13.5 lumbar DRG from wild-type (A, B, and C), TauEWS-Pea3/+ Isl1Cre/+ (D, E, and F), or Bax−/− (G, H, and I) embryos cultured for 48 h without neurotrophic support (A, D, and G) or in the presence of NGF (B, E, and H) or NT-3 (C, F, and I) were stained for expression of neurofilament to visualize axonal extensions.
Scale bar: 130 μm. To directly compare neurotrophin dependence of DRG neurons expressing EWS-Pea3 from the Tau locus at a precocious versus isochronic time of onset, we generated a strain of mice in which Cre recombinase is expressed from the PV locus (Figure S4). |
T6967 |
2127-2129 |
TO |
denotes |
To |
T6969 |
2130-2138 |
RB |
denotes |
directly |
T6968 |
2139-2146 |
VB |
denotes |
compare |
T6971 |
2147-2159 |
NN |
denotes |
neurotrophin |
T6972 |
2160-2170 |
NN |
denotes |
dependence |
T6973 |
2171-2173 |
IN |
denotes |
of |
T6974 |
2174-2177 |
NN |
denotes |
DRG |
T6975 |
2178-2185 |
NNS |
denotes |
neurons |
T6976 |
2186-2196 |
VBG |
denotes |
expressing |
T6977 |
2197-2200 |
NN |
denotes |
EWS |
T6979 |
2200-2201 |
HYPH |
denotes |
- |
T6978 |
2201-2205 |
NN |
denotes |
Pea3 |
T6980 |
2206-2210 |
IN |
denotes |
from |
T6981 |
2211-2214 |
DT |
denotes |
the |
T6983 |
2215-2218 |
NN |
denotes |
Tau |
T6982 |
2219-2224 |
NN |
denotes |
locus |
T6984 |
2225-2227 |
IN |
denotes |
at |
T6985 |
2228-2229 |
DT |
denotes |
a |
T6987 |
2230-2240 |
JJ |
denotes |
precocious |
T6988 |
2241-2247 |
CC |
denotes |
versus |
T6989 |
2248-2258 |
JJ |
denotes |
isochronic |
T6986 |
2259-2263 |
NN |
denotes |
time |
T6990 |
2264-2266 |
IN |
denotes |
of |
T6991 |
2267-2272 |
NN |
denotes |
onset |
T6992 |
2272-2274 |
, |
denotes |
, |
T6993 |
2274-2276 |
PRP |
denotes |
we |
T6970 |
2277-2286 |
VBD |
denotes |
generated |
T6994 |
2287-2288 |
DT |
denotes |
a |
T6995 |
2289-2295 |
NN |
denotes |
strain |
T6996 |
2296-2298 |
IN |
denotes |
of |
T6997 |
2299-2303 |
NNS |
denotes |
mice |
T6998 |
2304-2306 |
IN |
denotes |
in |
T7000 |
2307-2312 |
WDT |
denotes |
which |
T7001 |
2313-2316 |
NN |
denotes |
Cre |
T7002 |
2317-2328 |
NN |
denotes |
recombinase |
T7003 |
2329-2331 |
VBZ |
denotes |
is |
T6999 |
2332-2341 |
VBN |
denotes |
expressed |
T7004 |
2342-2346 |
IN |
denotes |
from |
T7005 |
2347-2350 |
DT |
denotes |
the |
T7007 |
2351-2353 |
NN |
denotes |
PV |
T7006 |
2354-2359 |
NN |
denotes |
locus |
T7008 |
2360-2361 |
-LRB- |
denotes |
( |
T7010 |
2361-2367 |
NN |
denotes |
Figure |
T7009 |
2368-2370 |
NN |
denotes |
S4 |
T7011 |
2370-2371 |
-RRB- |
denotes |
) |
T7012 |
2371-2372 |
. |
denotes |
. |
T7013 |
2372-2557 |
sentence |
denotes |
The expression of GFP in TaumGFP/+ PVCre/+ was restricted to PV+ proprioceptive DRG neurons and mirrored the onset of expression of PV at approximately E14 (Figure S4; data not shown). |
T7014 |
2373-2376 |
DT |
denotes |
The |
T7015 |
2377-2387 |
NN |
denotes |
expression |
T7017 |
2388-2390 |
IN |
denotes |
of |
T7018 |
2391-2394 |
NN |
denotes |
GFP |
T7019 |
2395-2397 |
IN |
denotes |
in |
T7020 |
2398-2405 |
NN |
denotes |
TaumGFP |
T7022 |
2405-2406 |
HYPH |
denotes |
/ |
T7023 |
2406-2407 |
SYM |
denotes |
+ |
T7021 |
2408-2413 |
NN |
denotes |
PVCre |
T7024 |
2413-2414 |
HYPH |
denotes |
/ |
T7025 |
2414-2415 |
SYM |
denotes |
+ |
T7026 |
2416-2419 |
VBD |
denotes |
was |
T7016 |
2420-2430 |
VBN |
denotes |
restricted |
T7027 |
2431-2433 |
IN |
denotes |
to |
T7028 |
2434-2436 |
NN |
denotes |
PV |
T7030 |
2436-2437 |
SYM |
denotes |
+ |
T7031 |
2438-2452 |
JJ |
denotes |
proprioceptive |
T7032 |
2453-2456 |
NN |
denotes |
DRG |
T7029 |
2457-2464 |
NNS |
denotes |
neurons |
T7033 |
2465-2468 |
CC |
denotes |
and |
T7034 |
2469-2477 |
VBD |
denotes |
mirrored |
T7035 |
2478-2481 |
DT |
denotes |
the |
T7036 |
2482-2487 |
NN |
denotes |
onset |
T7037 |
2488-2490 |
IN |
denotes |
of |
T7038 |
2491-2501 |
NN |
denotes |
expression |
T7039 |
2502-2504 |
IN |
denotes |
of |
T7040 |
2505-2507 |
NN |
denotes |
PV |
T7041 |
2508-2510 |
IN |
denotes |
at |
T7042 |
2511-2524 |
RB |
denotes |
approximately |
T7043 |
2525-2528 |
NN |
denotes |
E14 |
T7044 |
2529-2530 |
-LRB- |
denotes |
( |
T7046 |
2530-2536 |
NN |
denotes |
Figure |
T7045 |
2537-2539 |
NN |
denotes |
S4 |
T7047 |
2539-2540 |
: |
denotes |
; |
T7048 |
2541-2545 |
NNS |
denotes |
data |
T7050 |
2546-2549 |
RB |
denotes |
not |
T7049 |
2550-2555 |
VBN |
denotes |
shown |
T7051 |
2555-2556 |
-RRB- |
denotes |
) |
T7052 |
2556-2557 |
. |
denotes |
. |
T7053 |
2557-2718 |
sentence |
denotes |
We next cultured E14.5 whole DRG explants from TauEWS-Pea3/+ PVCre/+ and TaumGFP/+ PVCre/+ mice for 48 h in vitro in the presence or absence of NT-3 (Figure 5). |
T7054 |
2558-2560 |
PRP |
denotes |
We |
T7056 |
2561-2565 |
RB |
denotes |
next |
T7055 |
2566-2574 |
VBN |
denotes |
cultured |
T7057 |
2575-2580 |
NN |
denotes |
E14.5 |
T7059 |
2581-2586 |
JJ |
denotes |
whole |
T7060 |
2587-2590 |
NN |
denotes |
DRG |
T7058 |
2591-2599 |
NNS |
denotes |
explants |
T7061 |
2600-2604 |
IN |
denotes |
from |
T7062 |
2605-2611 |
NN |
denotes |
TauEWS |
T7064 |
2611-2612 |
HYPH |
denotes |
- |
T7065 |
2612-2616 |
NN |
denotes |
Pea3 |
T7066 |
2616-2617 |
HYPH |
denotes |
/ |
T7067 |
2617-2618 |
SYM |
denotes |
+ |
T7063 |
2619-2624 |
NN |
denotes |
PVCre |
T7069 |
2624-2625 |
HYPH |
denotes |
/ |
T7070 |
2625-2626 |
SYM |
denotes |
+ |
T7071 |
2627-2630 |
CC |
denotes |
and |
T7072 |
2631-2638 |
NN |
denotes |
TaumGFP |
T7074 |
2638-2639 |
HYPH |
denotes |
/ |
T7075 |
2639-2640 |
SYM |
denotes |
+ |
T7073 |
2641-2646 |
NN |
denotes |
PVCre |
T7076 |
2646-2647 |
HYPH |
denotes |
/ |
T7077 |
2647-2648 |
SYM |
denotes |
+ |
T7068 |
2649-2653 |
NNS |
denotes |
mice |
T7078 |
2654-2657 |
IN |
denotes |
for |
T7079 |
2658-2660 |
CD |
denotes |
48 |
T7080 |
2661-2662 |
NN |
denotes |
h |
T7081 |
2663-2665 |
FW |
denotes |
in |
T7082 |
2666-2671 |
FW |
denotes |
vitro |
T7083 |
2672-2674 |
IN |
denotes |
in |
T7084 |
2675-2678 |
DT |
denotes |
the |
T7085 |
2679-2687 |
NN |
denotes |
presence |
T7086 |
2688-2690 |
CC |
denotes |
or |
T7087 |
2691-2698 |
NN |
denotes |
absence |
T7088 |
2699-2701 |
IN |
denotes |
of |
T7089 |
2702-2704 |
NN |
denotes |
NT |
T7090 |
2704-2705 |
HYPH |
denotes |
- |
T7091 |
2705-2706 |
CD |
denotes |
3 |
T7092 |
2707-2708 |
-LRB- |
denotes |
( |
T7093 |
2708-2714 |
NN |
denotes |
Figure |
T7094 |
2715-2716 |
CD |
denotes |
5 |
T7095 |
2716-2717 |
-RRB- |
denotes |
) |
T7096 |
2717-2718 |
. |
denotes |
. |
T7097 |
2718-2878 |
sentence |
denotes |
We found that DRG neurons from both genotypes survived and extended neurites only in the presence of NT-3, whereas they died in the absence of NT-3 (Figure 5). |
T7098 |
2719-2721 |
PRP |
denotes |
We |
T7099 |
2722-2727 |
VBD |
denotes |
found |
T7100 |
2728-2732 |
IN |
denotes |
that |
T7102 |
2733-2736 |
NN |
denotes |
DRG |
T7103 |
2737-2744 |
NNS |
denotes |
neurons |
T7104 |
2745-2749 |
IN |
denotes |
from |
T7105 |
2750-2754 |
DT |
denotes |
both |
T7106 |
2755-2764 |
NNS |
denotes |
genotypes |
T7101 |
2765-2773 |
VBD |
denotes |
survived |
T7107 |
2774-2777 |
CC |
denotes |
and |
T7108 |
2778-2786 |
VBD |
denotes |
extended |
T7109 |
2787-2795 |
NNS |
denotes |
neurites |
T7110 |
2796-2800 |
RB |
denotes |
only |
T7111 |
2801-2803 |
IN |
denotes |
in |
T7112 |
2804-2807 |
DT |
denotes |
the |
T7113 |
2808-2816 |
NN |
denotes |
presence |
T7114 |
2817-2819 |
IN |
denotes |
of |
T7115 |
2820-2822 |
NN |
denotes |
NT |
T7116 |
2822-2823 |
HYPH |
denotes |
- |
T7117 |
2823-2824 |
CD |
denotes |
3 |
T7118 |
2824-2826 |
, |
denotes |
, |
T7119 |
2826-2833 |
IN |
denotes |
whereas |
T7121 |
2834-2838 |
PRP |
denotes |
they |
T7120 |
2839-2843 |
VBD |
denotes |
died |
T7122 |
2844-2846 |
IN |
denotes |
in |
T7123 |
2847-2850 |
DT |
denotes |
the |
T7124 |
2851-2858 |
NN |
denotes |
absence |
T7125 |
2859-2861 |
IN |
denotes |
of |
T7126 |
2862-2864 |
NN |
denotes |
NT |
T7127 |
2864-2865 |
HYPH |
denotes |
- |
T7128 |
2865-2866 |
CD |
denotes |
3 |
T7129 |
2867-2868 |
-LRB- |
denotes |
( |
T7130 |
2868-2874 |
NN |
denotes |
Figure |
T7131 |
2875-2876 |
CD |
denotes |
5 |
T7132 |
2876-2877 |
-RRB- |
denotes |
) |
T7133 |
2877-2878 |
. |
denotes |
. |
T7134 |
2878-3118 |
sentence |
denotes |
Together, these findings suggest that only precocious but not isochronic ETS signaling in DRG neurons is capable of uncoupling survival and neurite outgrowth from a requirement for neurotrophin signaling normally observed in wild-type DRG. |
T7135 |
2879-2887 |
RB |
denotes |
Together |
T7137 |
2887-2889 |
, |
denotes |
, |
T7138 |
2889-2894 |
DT |
denotes |
these |
T7139 |
2895-2903 |
NNS |
denotes |
findings |
T7136 |
2904-2911 |
VBP |
denotes |
suggest |
T7140 |
2912-2916 |
IN |
denotes |
that |
T7142 |
2917-2921 |
RB |
denotes |
only |
T7144 |
2922-2932 |
JJ |
denotes |
precocious |
T7145 |
2933-2936 |
CC |
denotes |
but |
T7146 |
2937-2940 |
RB |
denotes |
not |
T7147 |
2941-2951 |
JJ |
denotes |
isochronic |
T7148 |
2952-2955 |
NN |
denotes |
ETS |
T7143 |
2956-2965 |
NN |
denotes |
signaling |
T7149 |
2966-2968 |
IN |
denotes |
in |
T7150 |
2969-2972 |
NN |
denotes |
DRG |
T7151 |
2973-2980 |
NNS |
denotes |
neurons |
T7141 |
2981-2983 |
VBZ |
denotes |
is |
T7152 |
2984-2991 |
JJ |
denotes |
capable |
T7153 |
2992-2994 |
IN |
denotes |
of |
T7154 |
2995-3005 |
VBG |
denotes |
uncoupling |
T7155 |
3006-3014 |
NN |
denotes |
survival |
T7156 |
3015-3018 |
CC |
denotes |
and |
T7157 |
3019-3026 |
NN |
denotes |
neurite |
T7158 |
3027-3036 |
NN |
denotes |
outgrowth |
T7159 |
3037-3041 |
IN |
denotes |
from |
T7160 |
3042-3043 |
DT |
denotes |
a |
T7161 |
3044-3055 |
NN |
denotes |
requirement |
T7162 |
3056-3059 |
IN |
denotes |
for |
T7163 |
3060-3072 |
NN |
denotes |
neurotrophin |
T7164 |
3073-3082 |
NN |
denotes |
signaling |
T7165 |
3083-3091 |
RB |
denotes |
normally |
T7166 |
3092-3100 |
VBN |
denotes |
observed |
T7167 |
3101-3103 |
IN |
denotes |
in |
T7168 |
3104-3108 |
JJ |
denotes |
wild |
T7170 |
3108-3109 |
HYPH |
denotes |
- |
T7169 |
3109-3113 |
NN |
denotes |
type |
T7171 |
3114-3117 |
NN |
denotes |
DRG |
T7172 |
3117-3118 |
. |
denotes |
. |
T7173 |
3118-3839 |
sentence |
denotes |
Figure 5 DRG Neurons Expressing EWS-Pea3 Isochronically Depend on Neurotrophins for Survival
E14.5 lumbar DRG from TaumGFP/+ PVCre/+ (A and B) and TauEWS-Pea3/+ PVCre/+ (C and D) embryos cultured for 48 h without neurotrophic support (A and C) or in the presence of NT-3 (B and D) were stained for expression of neurofilament (red) and LacZ (green) to visualize axonal extensions and survival of PV-expressing proprioceptive afferents.
Scale bar: 150 μm. To determine whether neuronal survival of DRG neurons from TauEWS-Pea3/+ Isl1Cre/+ embryos in the absence of neurotrophic support is sufficient to explain the observed neuronal outgrowth, we analyzed DRG isolated from mice mutant in the proapoptotic gene Bax [27]. |
T7174 |
3575-3577 |
TO |
denotes |
To |
T7175 |
3578-3587 |
VB |
denotes |
determine |
T7177 |
3588-3595 |
IN |
denotes |
whether |
T7179 |
3596-3604 |
JJ |
denotes |
neuronal |
T7180 |
3605-3613 |
NN |
denotes |
survival |
T7181 |
3614-3616 |
IN |
denotes |
of |
T7182 |
3617-3620 |
NN |
denotes |
DRG |
T7183 |
3621-3628 |
NNS |
denotes |
neurons |
T7184 |
3629-3633 |
IN |
denotes |
from |
T7185 |
3634-3640 |
NN |
denotes |
TauEWS |
T7187 |
3640-3641 |
HYPH |
denotes |
- |
T7188 |
3641-3645 |
NN |
denotes |
Pea3 |
T7189 |
3645-3646 |
HYPH |
denotes |
/ |
T7190 |
3646-3647 |
SYM |
denotes |
+ |
T7186 |
3648-3655 |
NN |
denotes |
Isl1Cre |
T7192 |
3655-3656 |
HYPH |
denotes |
/ |
T7193 |
3656-3657 |
SYM |
denotes |
+ |
T7191 |
3658-3665 |
NNS |
denotes |
embryos |
T7194 |
3666-3668 |
IN |
denotes |
in |
T7195 |
3669-3672 |
DT |
denotes |
the |
T7196 |
3673-3680 |
NN |
denotes |
absence |
T7197 |
3681-3683 |
IN |
denotes |
of |
T7198 |
3684-3696 |
JJ |
denotes |
neurotrophic |
T7199 |
3697-3704 |
NN |
denotes |
support |
T7178 |
3705-3707 |
VBZ |
denotes |
is |
T7200 |
3708-3718 |
JJ |
denotes |
sufficient |
T7201 |
3719-3721 |
TO |
denotes |
to |
T7202 |
3722-3729 |
VB |
denotes |
explain |
T7203 |
3730-3733 |
DT |
denotes |
the |
T7205 |
3734-3742 |
VBN |
denotes |
observed |
T7206 |
3743-3751 |
JJ |
denotes |
neuronal |
T7204 |
3752-3761 |
NN |
denotes |
outgrowth |
T7207 |
3761-3763 |
, |
denotes |
, |
T7208 |
3763-3765 |
PRP |
denotes |
we |
T7176 |
3766-3774 |
VBD |
denotes |
analyzed |
T7209 |
3775-3778 |
NN |
denotes |
DRG |
T7210 |
3779-3787 |
VBN |
denotes |
isolated |
T7211 |
3788-3792 |
IN |
denotes |
from |
T7212 |
3793-3797 |
NNS |
denotes |
mice |
T7213 |
3798-3804 |
NN |
denotes |
mutant |
T7214 |
3805-3807 |
IN |
denotes |
in |
T7215 |
3808-3811 |
DT |
denotes |
the |
T7217 |
3812-3824 |
JJ |
denotes |
proapoptotic |
T7218 |
3825-3829 |
NN |
denotes |
gene |
T7216 |
3830-3833 |
NN |
denotes |
Bax |
T7219 |
3834-3835 |
-LRB- |
denotes |
[ |
T7220 |
3835-3837 |
CD |
denotes |
27 |
T7221 |
3837-3838 |
-RRB- |
denotes |
] |
T7222 |
3838-3839 |
. |
denotes |
. |
T7223 |
3839-3936 |
sentence |
denotes |
Consistent with previous results, Bax−/− DRG neurons survived without neurotrophic support [28]. |
T7224 |
3840-3850 |
JJ |
denotes |
Consistent |
T7226 |
3851-3855 |
IN |
denotes |
with |
T7227 |
3856-3864 |
JJ |
denotes |
previous |
T7228 |
3865-3872 |
NNS |
denotes |
results |
T7229 |
3872-3874 |
, |
denotes |
, |
T7230 |
3874-3877 |
NN |
denotes |
Bax |
T7232 |
3877-3878 |
SYM |
denotes |
− |
T7233 |
3878-3879 |
HYPH |
denotes |
/ |
T7234 |
3879-3880 |
SYM |
denotes |
− |
T7235 |
3881-3884 |
NN |
denotes |
DRG |
T7231 |
3885-3892 |
NNS |
denotes |
neurons |
T7225 |
3893-3901 |
VBD |
denotes |
survived |
T7236 |
3902-3909 |
IN |
denotes |
without |
T7237 |
3910-3922 |
JJ |
denotes |
neurotrophic |
T7238 |
3923-3930 |
NN |
denotes |
support |
T7239 |
3931-3932 |
-LRB- |
denotes |
[ |
T7240 |
3932-3934 |
CD |
denotes |
28 |
T7241 |
3934-3935 |
-RRB- |
denotes |
] |
T7242 |
3935-3936 |
. |
denotes |
. |
T7243 |
3936-4248 |
sentence |
denotes |
In contrast, neurite outgrowth of Bax−/− DRG neurons was significantly less (see Figure 4G) than that of either DRG from TauEWS-Pea3/+ Isl1Cre/+ embryos cultured in the absence of neurotrophic support (see Figure 4D) or Bax−/− DRG neurons cultured in the presence of neurotrophic support (see Figure 4H and 4I). |
T7244 |
3937-3939 |
IN |
denotes |
In |
T7246 |
3940-3948 |
NN |
denotes |
contrast |
T7247 |
3948-3950 |
, |
denotes |
, |
T7248 |
3950-3957 |
NN |
denotes |
neurite |
T7249 |
3958-3967 |
NN |
denotes |
outgrowth |
T7250 |
3968-3970 |
IN |
denotes |
of |
T7251 |
3971-3974 |
NN |
denotes |
Bax |
T7253 |
3974-3975 |
SYM |
denotes |
− |
T7254 |
3975-3976 |
HYPH |
denotes |
/ |
T7255 |
3976-3977 |
SYM |
denotes |
− |
T7256 |
3978-3981 |
NN |
denotes |
DRG |
T7252 |
3982-3989 |
NNS |
denotes |
neurons |
T7245 |
3990-3993 |
VBD |
denotes |
was |
T7257 |
3994-4007 |
RB |
denotes |
significantly |
T7258 |
4008-4012 |
JJR |
denotes |
less |
T7259 |
4013-4014 |
-LRB- |
denotes |
( |
T7260 |
4014-4017 |
VB |
denotes |
see |
T7261 |
4018-4024 |
NN |
denotes |
Figure |
T7262 |
4025-4027 |
NN |
denotes |
4G |
T7263 |
4027-4028 |
-RRB- |
denotes |
) |
T7264 |
4029-4033 |
IN |
denotes |
than |
T7265 |
4034-4038 |
DT |
denotes |
that |
T7266 |
4039-4041 |
IN |
denotes |
of |
T7267 |
4042-4048 |
CC |
denotes |
either |
T7268 |
4049-4052 |
NN |
denotes |
DRG |
T7269 |
4053-4057 |
IN |
denotes |
from |
T7270 |
4058-4064 |
NN |
denotes |
TauEWS |
T7272 |
4064-4065 |
HYPH |
denotes |
- |
T7273 |
4065-4069 |
NN |
denotes |
Pea3 |
T7274 |
4069-4070 |
HYPH |
denotes |
/ |
T7275 |
4070-4071 |
SYM |
denotes |
+ |
T7271 |
4072-4079 |
NN |
denotes |
Isl1Cre |
T7277 |
4079-4080 |
HYPH |
denotes |
/ |
T7278 |
4080-4081 |
SYM |
denotes |
+ |
T7276 |
4082-4089 |
NNS |
denotes |
embryos |
T7279 |
4090-4098 |
VBN |
denotes |
cultured |
T7280 |
4099-4101 |
IN |
denotes |
in |
T7281 |
4102-4105 |
DT |
denotes |
the |
T7282 |
4106-4113 |
NN |
denotes |
absence |
T7283 |
4114-4116 |
IN |
denotes |
of |
T7284 |
4117-4129 |
JJ |
denotes |
neurotrophic |
T7285 |
4130-4137 |
NN |
denotes |
support |
T7286 |
4138-4139 |
-LRB- |
denotes |
( |
T7287 |
4139-4142 |
VB |
denotes |
see |
T7288 |
4143-4149 |
NN |
denotes |
Figure |
T7289 |
4150-4152 |
NN |
denotes |
4D |
T7290 |
4152-4153 |
-RRB- |
denotes |
) |
T7291 |
4154-4156 |
CC |
denotes |
or |
T7292 |
4157-4160 |
NN |
denotes |
Bax |
T7294 |
4160-4161 |
SYM |
denotes |
− |
T7295 |
4161-4162 |
HYPH |
denotes |
/ |
T7296 |
4162-4163 |
SYM |
denotes |
− |
T7297 |
4164-4167 |
NN |
denotes |
DRG |
T7293 |
4168-4175 |
NNS |
denotes |
neurons |
T7298 |
4176-4184 |
VBN |
denotes |
cultured |
T7299 |
4185-4187 |
IN |
denotes |
in |
T7300 |
4188-4191 |
DT |
denotes |
the |
T7301 |
4192-4200 |
NN |
denotes |
presence |
T7302 |
4201-4203 |
IN |
denotes |
of |
T7303 |
4204-4216 |
JJ |
denotes |
neurotrophic |
T7304 |
4217-4224 |
NN |
denotes |
support |
T7305 |
4225-4226 |
-LRB- |
denotes |
( |
T7306 |
4226-4229 |
VB |
denotes |
see |
T7307 |
4230-4236 |
NN |
denotes |
Figure |
T7308 |
4237-4239 |
NN |
denotes |
4H |
T7309 |
4240-4243 |
CC |
denotes |
and |
T7310 |
4244-4246 |
NN |
denotes |
4I |
T7311 |
4246-4247 |
-RRB- |
denotes |
) |
T7312 |
4247-4248 |
. |
denotes |
. |
T7313 |
4248-4468 |
sentence |
denotes |
These findings suggest that in addition to mediating neurotrophin-independent neuronal survival, expression of EWS-Pea3 in early post-mitotic neurons also promotes neurite outgrowth in a neurotrophin-independent manner. |
T7314 |
4249-4254 |
DT |
denotes |
These |
T7315 |
4255-4263 |
NNS |
denotes |
findings |
T7316 |
4264-4271 |
VBP |
denotes |
suggest |
T7317 |
4272-4276 |
IN |
denotes |
that |
T7319 |
4277-4279 |
IN |
denotes |
in |
T7320 |
4280-4288 |
NN |
denotes |
addition |
T7321 |
4289-4291 |
IN |
denotes |
to |
T7322 |
4292-4301 |
VBG |
denotes |
mediating |
T7323 |
4302-4314 |
NN |
denotes |
neurotrophin |
T7325 |
4314-4315 |
HYPH |
denotes |
- |
T7324 |
4315-4326 |
JJ |
denotes |
independent |
T7327 |
4327-4335 |
JJ |
denotes |
neuronal |
T7326 |
4336-4344 |
NN |
denotes |
survival |
T7328 |
4344-4346 |
, |
denotes |
, |
T7329 |
4346-4356 |
NN |
denotes |
expression |
T7330 |
4357-4359 |
IN |
denotes |
of |
T7331 |
4360-4363 |
NN |
denotes |
EWS |
T7333 |
4363-4364 |
HYPH |
denotes |
- |
T7332 |
4364-4368 |
NN |
denotes |
Pea3 |
T7334 |
4369-4371 |
IN |
denotes |
in |
T7335 |
4372-4377 |
JJ |
denotes |
early |
T7337 |
4378-4390 |
JJ |
denotes |
post-mitotic |
T7336 |
4391-4398 |
NNS |
denotes |
neurons |
T7338 |
4399-4403 |
RB |
denotes |
also |
T7318 |
4404-4412 |
VBZ |
denotes |
promotes |
T7339 |
4413-4420 |
NN |
denotes |
neurite |
T7340 |
4421-4430 |
NN |
denotes |
outgrowth |
T7341 |
4431-4433 |
IN |
denotes |
in |
T7342 |
4434-4435 |
DT |
denotes |
a |
T7344 |
4436-4448 |
NN |
denotes |
neurotrophin |
T7346 |
4448-4449 |
HYPH |
denotes |
- |
T7345 |
4449-4460 |
JJ |
denotes |
independent |
T7343 |
4461-4467 |
NN |
denotes |
manner |
T7347 |
4467-4468 |
. |
denotes |
. |
T7348 |
4468-4741 |
sentence |
denotes |
To begin to assess at which step of the neurotrophin signaling cascade DRG neurons from TauEWS-Pea3/+ Isl1Cre/+ embryos become unresponsive to the addition of neurotrophins, we assayed the expression of neurotrophin receptors in TauEWS-Pea3/+ Isl1Cre/+ embryos (Figure 6). |
T7349 |
4469-4471 |
TO |
denotes |
To |
T7350 |
4472-4477 |
VB |
denotes |
begin |
T7352 |
4478-4480 |
TO |
denotes |
to |
T7353 |
4481-4487 |
VB |
denotes |
assess |
T7354 |
4488-4490 |
IN |
denotes |
at |
T7356 |
4491-4496 |
WDT |
denotes |
which |
T7357 |
4497-4501 |
NN |
denotes |
step |
T7358 |
4502-4504 |
IN |
denotes |
of |
T7359 |
4505-4508 |
DT |
denotes |
the |
T7361 |
4509-4521 |
NN |
denotes |
neurotrophin |
T7362 |
4522-4531 |
NN |
denotes |
signaling |
T7360 |
4532-4539 |
NN |
denotes |
cascade |
T7363 |
4540-4543 |
NN |
denotes |
DRG |
T7364 |
4544-4551 |
NNS |
denotes |
neurons |
T7365 |
4552-4556 |
IN |
denotes |
from |
T7366 |
4557-4563 |
NN |
denotes |
TauEWS |
T7368 |
4563-4564 |
HYPH |
denotes |
- |
T7369 |
4564-4568 |
NN |
denotes |
Pea3 |
T7370 |
4568-4569 |
HYPH |
denotes |
/ |
T7371 |
4569-4570 |
SYM |
denotes |
+ |
T7367 |
4571-4578 |
NN |
denotes |
Isl1Cre |
T7373 |
4578-4579 |
HYPH |
denotes |
/ |
T7374 |
4579-4580 |
SYM |
denotes |
+ |
T7372 |
4581-4588 |
NNS |
denotes |
embryos |
T7355 |
4589-4595 |
VBP |
denotes |
become |
T7375 |
4596-4608 |
JJ |
denotes |
unresponsive |
T7376 |
4609-4611 |
IN |
denotes |
to |
T7377 |
4612-4615 |
DT |
denotes |
the |
T7378 |
4616-4624 |
NN |
denotes |
addition |
T7379 |
4625-4627 |
IN |
denotes |
of |
T7380 |
4628-4641 |
NNS |
denotes |
neurotrophins |
T7381 |
4641-4643 |
, |
denotes |
, |
T7382 |
4643-4645 |
PRP |
denotes |
we |
T7351 |
4646-4653 |
VBD |
denotes |
assayed |
T7383 |
4654-4657 |
DT |
denotes |
the |
T7384 |
4658-4668 |
NN |
denotes |
expression |
T7385 |
4669-4671 |
IN |
denotes |
of |
T7386 |
4672-4684 |
NN |
denotes |
neurotrophin |
T7387 |
4685-4694 |
NNS |
denotes |
receptors |
T7388 |
4695-4697 |
IN |
denotes |
in |
T7389 |
4698-4704 |
NN |
denotes |
TauEWS |
T7391 |
4704-4705 |
HYPH |
denotes |
- |
T7392 |
4705-4709 |
NN |
denotes |
Pea3 |
T7393 |
4709-4710 |
HYPH |
denotes |
/ |
T7394 |
4710-4711 |
SYM |
denotes |
+ |
T7390 |
4712-4719 |
NN |
denotes |
Isl1Cre |
T7396 |
4719-4720 |
HYPH |
denotes |
/ |
T7397 |
4720-4721 |
SYM |
denotes |
+ |
T7395 |
4722-4729 |
NNS |
denotes |
embryos |
T7398 |
4730-4731 |
-LRB- |
denotes |
( |
T7399 |
4731-4737 |
NN |
denotes |
Figure |
T7400 |
4738-4739 |
CD |
denotes |
6 |
T7401 |
4739-4740 |
-RRB- |
denotes |
) |
T7402 |
4740-4741 |
. |
denotes |
. |
T7403 |
4741-5043 |
sentence |
denotes |
Whereas expression of the neurotrophin receptors TrkA, TrkB, and TrkC marks afferents of distinct sensory modalities in DRG of wild-type embryos (Figure 6A–6C) [4,29], TauEWS-Pea3/+ Isl1Cre/+ embryos showed complete absence of expression of TrkA, TrkB, and TrkC in DRG neurons at E16.5 (Figure 6G–6I). |
T7404 |
4742-4749 |
IN |
denotes |
Whereas |
T7406 |
4750-4760 |
NN |
denotes |
expression |
T7407 |
4761-4763 |
IN |
denotes |
of |
T7408 |
4764-4767 |
DT |
denotes |
the |
T7410 |
4768-4780 |
NN |
denotes |
neurotrophin |
T7409 |
4781-4790 |
NNS |
denotes |
receptors |
T7411 |
4791-4795 |
NN |
denotes |
TrkA |
T7412 |
4795-4797 |
, |
denotes |
, |
T7413 |
4797-4801 |
NN |
denotes |
TrkB |
T7414 |
4801-4803 |
, |
denotes |
, |
T7415 |
4803-4806 |
CC |
denotes |
and |
T7416 |
4807-4811 |
NN |
denotes |
TrkC |
T7405 |
4812-4817 |
VBZ |
denotes |
marks |
T7418 |
4818-4827 |
NNS |
denotes |
afferents |
T7419 |
4828-4830 |
IN |
denotes |
of |
T7420 |
4831-4839 |
JJ |
denotes |
distinct |
T7422 |
4840-4847 |
JJ |
denotes |
sensory |
T7421 |
4848-4858 |
NNS |
denotes |
modalities |
T7423 |
4859-4861 |
IN |
denotes |
in |
T7424 |
4862-4865 |
NN |
denotes |
DRG |
T7425 |
4866-4868 |
IN |
denotes |
of |
T7426 |
4869-4873 |
JJ |
denotes |
wild |
T7428 |
4873-4874 |
HYPH |
denotes |
- |
T7427 |
4874-4878 |
NN |
denotes |
type |
T7429 |
4879-4886 |
NNS |
denotes |
embryos |
T7430 |
4887-4888 |
-LRB- |
denotes |
( |
T7432 |
4888-4894 |
NN |
denotes |
Figure |
T7431 |
4895-4897 |
NN |
denotes |
6A |
T7433 |
4897-4898 |
SYM |
denotes |
– |
T7434 |
4898-4900 |
NN |
denotes |
6C |
T7435 |
4900-4901 |
-RRB- |
denotes |
) |
T7436 |
4902-4903 |
-LRB- |
denotes |
[ |
T7438 |
4903-4904 |
CD |
denotes |
4 |
T7439 |
4904-4905 |
, |
denotes |
, |
T7437 |
4905-4907 |
CD |
denotes |
29 |
T7440 |
4907-4908 |
-RRB- |
denotes |
] |
T7441 |
4908-4910 |
, |
denotes |
, |
T7442 |
4910-4916 |
NN |
denotes |
TauEWS |
T7444 |
4916-4917 |
HYPH |
denotes |
- |
T7445 |
4917-4921 |
NN |
denotes |
Pea3 |
T7446 |
4921-4922 |
HYPH |
denotes |
/ |
T7447 |
4922-4923 |
SYM |
denotes |
+ |
T7443 |
4924-4931 |
NN |
denotes |
Isl1Cre |
T7449 |
4931-4932 |
HYPH |
denotes |
/ |
T7450 |
4932-4933 |
SYM |
denotes |
+ |
T7448 |
4934-4941 |
NNS |
denotes |
embryos |
T7417 |
4942-4948 |
VBD |
denotes |
showed |
T7451 |
4949-4957 |
JJ |
denotes |
complete |
T7452 |
4958-4965 |
NN |
denotes |
absence |
T7453 |
4966-4968 |
IN |
denotes |
of |
T7454 |
4969-4979 |
NN |
denotes |
expression |
T7455 |
4980-4982 |
IN |
denotes |
of |
T7456 |
4983-4987 |
NN |
denotes |
TrkA |
T7457 |
4987-4989 |
, |
denotes |
, |
T7458 |
4989-4993 |
NN |
denotes |
TrkB |
T7459 |
4993-4995 |
, |
denotes |
, |
T7460 |
4995-4998 |
CC |
denotes |
and |
T7461 |
4999-5003 |
NN |
denotes |
TrkC |
T7462 |
5004-5006 |
IN |
denotes |
in |
T7463 |
5007-5010 |
NN |
denotes |
DRG |
T7464 |
5011-5018 |
NNS |
denotes |
neurons |
T7465 |
5019-5021 |
IN |
denotes |
at |
T7466 |
5022-5027 |
NN |
denotes |
E16.5 |
T7467 |
5028-5029 |
-LRB- |
denotes |
( |
T7469 |
5029-5035 |
NN |
denotes |
Figure |
T7468 |
5036-5038 |
NN |
denotes |
6G |
T7470 |
5038-5039 |
SYM |
denotes |
– |
T7471 |
5039-5041 |
NN |
denotes |
6I |
T7472 |
5041-5042 |
-RRB- |
denotes |
) |
T7473 |
5042-5043 |
. |
denotes |
. |
T7474 |
5043-5244 |
sentence |
denotes |
This absence of Trk receptor expression in DRG of TauEWS-Pea3/+ Isl1Cre/+ embryos provides a likely explanation for the lack of responsiveness of these neurons to the addition of neurotrophic factors. |
T7475 |
5044-5048 |
DT |
denotes |
This |
T7476 |
5049-5056 |
NN |
denotes |
absence |
T7478 |
5057-5059 |
IN |
denotes |
of |
T7479 |
5060-5063 |
NN |
denotes |
Trk |
T7481 |
5064-5072 |
NN |
denotes |
receptor |
T7480 |
5073-5083 |
NN |
denotes |
expression |
T7482 |
5084-5086 |
IN |
denotes |
in |
T7483 |
5087-5090 |
NN |
denotes |
DRG |
T7484 |
5091-5093 |
IN |
denotes |
of |
T7485 |
5094-5100 |
NN |
denotes |
TauEWS |
T7487 |
5100-5101 |
HYPH |
denotes |
- |
T7488 |
5101-5105 |
NN |
denotes |
Pea3 |
T7489 |
5105-5106 |
HYPH |
denotes |
/ |
T7490 |
5106-5107 |
SYM |
denotes |
+ |
T7486 |
5108-5115 |
NN |
denotes |
Isl1Cre |
T7492 |
5115-5116 |
HYPH |
denotes |
/ |
T7493 |
5116-5117 |
SYM |
denotes |
+ |
T7491 |
5118-5125 |
NNS |
denotes |
embryos |
T7477 |
5126-5134 |
VBZ |
denotes |
provides |
T7494 |
5135-5136 |
DT |
denotes |
a |
T7496 |
5137-5143 |
JJ |
denotes |
likely |
T7495 |
5144-5155 |
NN |
denotes |
explanation |
T7497 |
5156-5159 |
IN |
denotes |
for |
T7498 |
5160-5163 |
DT |
denotes |
the |
T7499 |
5164-5168 |
NN |
denotes |
lack |
T7500 |
5169-5171 |
IN |
denotes |
of |
T7501 |
5172-5186 |
NN |
denotes |
responsiveness |
T7502 |
5187-5189 |
IN |
denotes |
of |
T7503 |
5190-5195 |
DT |
denotes |
these |
T7504 |
5196-5203 |
NNS |
denotes |
neurons |
T7505 |
5204-5206 |
IN |
denotes |
to |
T7506 |
5207-5210 |
DT |
denotes |
the |
T7507 |
5211-5219 |
NN |
denotes |
addition |
T7508 |
5220-5222 |
IN |
denotes |
of |
T7509 |
5223-5235 |
JJ |
denotes |
neurotrophic |
T7510 |
5236-5243 |
NNS |
denotes |
factors |
T7511 |
5243-5244 |
. |
denotes |
. |
T7512 |
5244-6849 |
sentence |
denotes |
Figure 6 Loss of Trk Receptor Expression and Increased Survival in DRG Neurons upon Precocious ETS Signaling
(A–C and G–I) In situ hybridization analysis of TrkA (A and G), TrkB (B and H), and TrkC (C and I) expression in E16.5 lumbar DRG of wild-type (A–C) and TauEWS-Pea3/+ Isl1Cre/+ (G–I) embryos.
(D–F and J–L) Analysis of lumbar DRG of wild-type (D), TaumGFP/+ IslCre/+ (E and F), and TauEWS-Pea3/+ Isl1Cre/+ (J, K, and L) embryos for (1) neuronal cell death at E13.5 by TUNEL (green; D and J), (2) cell survival and proliferation at E16.5 by LacZ (blue) wholemount staining (E and K; lumbar levels L1 and L2 are shown), and (3) BrdU (green)/LacZ (red) double labeling (F and L).
(M and N) Quantitative analysis (n ≥ 3 independent experiments) of the mean number of apoptotic events relative to wild-type levels is shown in (M) and neuronal survival in (N) as percent of wild-type of DRG at lumbar levels L1 to L5 as quantified on serial sections.
(O) Western blot analysis of protein extracts isolated from lumbar DRG of E16.5 wild-type (wt) and TauEWS-Pea3/+ Isl1Cre/+ (mut) embryos using the following antibodies: Akt, p-Akt (Ser473), CREB, p-CREB (Ser133), Bax, Bcl2, and Bcl-xl.
(P) Quantitative analysis of protein levels relative to wild-type in percent is shown on the right (n = 3 independent experiments).
Scale bar: (A–C and G–I), 35 μm; (D and J), 40 μm; (E and K), 200 μm; (F and L), 50 μm. We next assayed whether the complete absence of Trk receptor expression in TauEWS-Pea3/+ Isl1Cre/+ embryos had an influence on naturally occurring cell death in vivo using TUNEL on DRG sections. |
T7513 |
6655-6657 |
PRP |
denotes |
We |
T7515 |
6658-6662 |
RB |
denotes |
next |
T7514 |
6663-6670 |
VBD |
denotes |
assayed |
T7516 |
6671-6678 |
IN |
denotes |
whether |
T7518 |
6679-6682 |
DT |
denotes |
the |
T7520 |
6683-6691 |
JJ |
denotes |
complete |
T7519 |
6692-6699 |
NN |
denotes |
absence |
T7521 |
6700-6702 |
IN |
denotes |
of |
T7522 |
6703-6706 |
NN |
denotes |
Trk |
T7524 |
6707-6715 |
NN |
denotes |
receptor |
T7523 |
6716-6726 |
NN |
denotes |
expression |
T7525 |
6727-6729 |
IN |
denotes |
in |
T7526 |
6730-6736 |
NN |
denotes |
TauEWS |
T7528 |
6736-6737 |
HYPH |
denotes |
- |
T7529 |
6737-6741 |
NN |
denotes |
Pea3 |
T7530 |
6741-6742 |
HYPH |
denotes |
/ |
T7531 |
6742-6743 |
SYM |
denotes |
+ |
T7527 |
6744-6751 |
NN |
denotes |
Isl1Cre |
T7533 |
6751-6752 |
HYPH |
denotes |
/ |
T7534 |
6752-6753 |
SYM |
denotes |
+ |
T7532 |
6754-6761 |
NNS |
denotes |
embryos |
T7517 |
6762-6765 |
VBD |
denotes |
had |
T7535 |
6766-6768 |
DT |
denotes |
an |
T7536 |
6769-6778 |
NN |
denotes |
influence |
T7537 |
6779-6781 |
IN |
denotes |
on |
T7538 |
6782-6791 |
RB |
denotes |
naturally |
T7539 |
6792-6801 |
VBG |
denotes |
occurring |
T7541 |
6802-6806 |
NN |
denotes |
cell |
T7540 |
6807-6812 |
NN |
denotes |
death |
T7542 |
6813-6815 |
FW |
denotes |
in |
T7543 |
6816-6820 |
FW |
denotes |
vivo |
T7544 |
6821-6826 |
VBG |
denotes |
using |
T7545 |
6827-6832 |
NN |
denotes |
TUNEL |
T7546 |
6833-6835 |
IN |
denotes |
on |
T7547 |
6836-6839 |
NN |
denotes |
DRG |
T7548 |
6840-6848 |
NNS |
denotes |
sections |
T7549 |
6848-6849 |
. |
denotes |
. |
T7550 |
6849-7057 |
sentence |
denotes |
Surprisingly, we found that apoptosis was decreased by approximately 50% (n = 3 embryos, average of >50 sections) in DRG of TauEWS-Pea3/+ Isl1Cre/+ embryos in comparison to wild-type (Figure 6D, 6J, and 6M). |
T7551 |
6850-6862 |
RB |
denotes |
Surprisingly |
T7553 |
6862-6864 |
, |
denotes |
, |
T7554 |
6864-6866 |
PRP |
denotes |
we |
T7552 |
6867-6872 |
VBD |
denotes |
found |
T7555 |
6873-6877 |
IN |
denotes |
that |
T7557 |
6878-6887 |
NN |
denotes |
apoptosis |
T7558 |
6888-6891 |
VBD |
denotes |
was |
T7556 |
6892-6901 |
VBN |
denotes |
decreased |
T7559 |
6902-6904 |
IN |
denotes |
by |
T7560 |
6905-6918 |
RB |
denotes |
approximately |
T7561 |
6919-6921 |
CD |
denotes |
50 |
T7562 |
6921-6922 |
NN |
denotes |
% |
T7563 |
6923-6924 |
-LRB- |
denotes |
( |
T7565 |
6924-6925 |
NN |
denotes |
n |
T7567 |
6926-6927 |
SYM |
denotes |
= |
T7568 |
6928-6929 |
CD |
denotes |
3 |
T7566 |
6930-6937 |
NNS |
denotes |
embryos |
T7569 |
6937-6939 |
, |
denotes |
, |
T7564 |
6939-6946 |
NN |
denotes |
average |
T7570 |
6947-6949 |
IN |
denotes |
of |
T7571 |
6950-6951 |
SYM |
denotes |
> |
T7572 |
6951-6953 |
CD |
denotes |
50 |
T7573 |
6954-6962 |
NNS |
denotes |
sections |
T7574 |
6962-6963 |
-RRB- |
denotes |
) |
T7575 |
6964-6966 |
IN |
denotes |
in |
T7576 |
6967-6970 |
NN |
denotes |
DRG |
T7577 |
6971-6973 |
IN |
denotes |
of |
T7578 |
6974-6980 |
NN |
denotes |
TauEWS |
T7580 |
6980-6981 |
HYPH |
denotes |
- |
T7581 |
6981-6985 |
NN |
denotes |
Pea3 |
T7582 |
6985-6986 |
HYPH |
denotes |
/ |
T7583 |
6986-6987 |
SYM |
denotes |
+ |
T7579 |
6988-6995 |
NN |
denotes |
Isl1Cre |
T7585 |
6995-6996 |
HYPH |
denotes |
/ |
T7586 |
6996-6997 |
SYM |
denotes |
+ |
T7584 |
6998-7005 |
NNS |
denotes |
embryos |
T7587 |
7006-7008 |
IN |
denotes |
in |
T7588 |
7009-7019 |
NN |
denotes |
comparison |
T7589 |
7020-7022 |
IN |
denotes |
to |
T7590 |
7023-7027 |
JJ |
denotes |
wild |
T7592 |
7027-7028 |
HYPH |
denotes |
- |
T7591 |
7028-7032 |
NN |
denotes |
type |
T7593 |
7033-7034 |
-LRB- |
denotes |
( |
T7595 |
7034-7040 |
NN |
denotes |
Figure |
T7594 |
7041-7043 |
NN |
denotes |
6D |
T7596 |
7043-7045 |
, |
denotes |
, |
T7597 |
7045-7047 |
NN |
denotes |
6J |
T7598 |
7047-7049 |
, |
denotes |
, |
T7599 |
7049-7052 |
CC |
denotes |
and |
T7600 |
7053-7055 |
NN |
denotes |
6M |
T7601 |
7055-7056 |
-RRB- |
denotes |
) |
T7602 |
7056-7057 |
. |
denotes |
. |
T7603 |
7057-7252 |
sentence |
denotes |
Quantitative analysis of the number of neurons in lumbar DRG of TauEWS-Pea3/+ Isl1Cre/+ embryos revealed a significant increase to approximately 170% of wild-type levels (Figure 6E, 6K, and 6N). |
T7604 |
7058-7070 |
JJ |
denotes |
Quantitative |
T7605 |
7071-7079 |
NN |
denotes |
analysis |
T7607 |
7080-7082 |
IN |
denotes |
of |
T7608 |
7083-7086 |
DT |
denotes |
the |
T7609 |
7087-7093 |
NN |
denotes |
number |
T7610 |
7094-7096 |
IN |
denotes |
of |
T7611 |
7097-7104 |
NNS |
denotes |
neurons |
T7612 |
7105-7107 |
IN |
denotes |
in |
T7613 |
7108-7114 |
NN |
denotes |
lumbar |
T7614 |
7115-7118 |
NN |
denotes |
DRG |
T7615 |
7119-7121 |
IN |
denotes |
of |
T7616 |
7122-7128 |
NN |
denotes |
TauEWS |
T7618 |
7128-7129 |
HYPH |
denotes |
- |
T7619 |
7129-7133 |
NN |
denotes |
Pea3 |
T7620 |
7133-7134 |
HYPH |
denotes |
/ |
T7621 |
7134-7135 |
SYM |
denotes |
+ |
T7617 |
7136-7143 |
NN |
denotes |
Isl1Cre |
T7623 |
7143-7144 |
HYPH |
denotes |
/ |
T7624 |
7144-7145 |
SYM |
denotes |
+ |
T7622 |
7146-7153 |
NNS |
denotes |
embryos |
T7606 |
7154-7162 |
VBD |
denotes |
revealed |
T7625 |
7163-7164 |
DT |
denotes |
a |
T7627 |
7165-7176 |
JJ |
denotes |
significant |
T7626 |
7177-7185 |
NN |
denotes |
increase |
T7628 |
7186-7188 |
IN |
denotes |
to |
T7629 |
7189-7202 |
RB |
denotes |
approximately |
T7630 |
7203-7206 |
CD |
denotes |
170 |
T7631 |
7206-7207 |
NN |
denotes |
% |
T7632 |
7208-7210 |
IN |
denotes |
of |
T7633 |
7211-7215 |
JJ |
denotes |
wild |
T7635 |
7215-7216 |
HYPH |
denotes |
- |
T7634 |
7216-7220 |
NN |
denotes |
type |
T7636 |
7221-7227 |
NNS |
denotes |
levels |
T7637 |
7228-7229 |
-LRB- |
denotes |
( |
T7639 |
7229-7235 |
NN |
denotes |
Figure |
T7638 |
7236-7238 |
NN |
denotes |
6E |
T7640 |
7238-7240 |
, |
denotes |
, |
T7641 |
7240-7242 |
NN |
denotes |
6K |
T7642 |
7242-7244 |
, |
denotes |
, |
T7643 |
7244-7247 |
CC |
denotes |
and |
T7644 |
7248-7250 |
NN |
denotes |
6N |
T7645 |
7250-7251 |
-RRB- |
denotes |
) |
T7646 |
7251-7252 |
. |
denotes |
. |
T7647 |
7252-7480 |
sentence |
denotes |
Moreover, BrdU pulse-chase experiments ruled out the possibility that DRG neurons in TauEWS-Pea3/+ Isl1Cre/+ embryos reenter the cell cycle (no BrdU+/LacZ+ cells, n = 3 embryos, analysis of >50 sections each; Figure 6F and 6L). |
T7648 |
7253-7261 |
RB |
denotes |
Moreover |
T7650 |
7261-7263 |
, |
denotes |
, |
T7651 |
7263-7267 |
NN |
denotes |
BrdU |
T7653 |
7268-7273 |
NN |
denotes |
pulse |
T7655 |
7273-7274 |
HYPH |
denotes |
- |
T7654 |
7274-7279 |
NN |
denotes |
chase |
T7652 |
7280-7291 |
NNS |
denotes |
experiments |
T7649 |
7292-7297 |
VBD |
denotes |
ruled |
T7656 |
7298-7301 |
RP |
denotes |
out |
T7657 |
7302-7305 |
DT |
denotes |
the |
T7658 |
7306-7317 |
NN |
denotes |
possibility |
T7659 |
7318-7322 |
IN |
denotes |
that |
T7661 |
7323-7326 |
NN |
denotes |
DRG |
T7662 |
7327-7334 |
NNS |
denotes |
neurons |
T7663 |
7335-7337 |
IN |
denotes |
in |
T7664 |
7338-7344 |
NN |
denotes |
TauEWS |
T7666 |
7344-7345 |
HYPH |
denotes |
- |
T7667 |
7345-7349 |
NN |
denotes |
Pea3 |
T7668 |
7349-7350 |
HYPH |
denotes |
/ |
T7669 |
7350-7351 |
SYM |
denotes |
+ |
T7665 |
7352-7359 |
NN |
denotes |
Isl1Cre |
T7671 |
7359-7360 |
HYPH |
denotes |
/ |
T7672 |
7360-7361 |
SYM |
denotes |
+ |
T7670 |
7362-7369 |
NNS |
denotes |
embryos |
T7660 |
7370-7377 |
VBP |
denotes |
reenter |
T7673 |
7378-7381 |
DT |
denotes |
the |
T7675 |
7382-7386 |
NN |
denotes |
cell |
T7674 |
7387-7392 |
NN |
denotes |
cycle |
T7676 |
7393-7394 |
-LRB- |
denotes |
( |
T7678 |
7394-7396 |
DT |
denotes |
no |
T7680 |
7397-7401 |
NN |
denotes |
BrdU |
T7682 |
7401-7402 |
SYM |
denotes |
+ |
T7683 |
7402-7403 |
HYPH |
denotes |
/ |
T7681 |
7403-7407 |
NN |
denotes |
LacZ |
T7684 |
7407-7408 |
SYM |
denotes |
+ |
T7679 |
7409-7414 |
NNS |
denotes |
cells |
T7685 |
7414-7416 |
, |
denotes |
, |
T7686 |
7416-7417 |
NN |
denotes |
n |
T7688 |
7418-7419 |
SYM |
denotes |
= |
T7689 |
7420-7421 |
CD |
denotes |
3 |
T7687 |
7422-7429 |
NNS |
denotes |
embryos |
T7690 |
7429-7431 |
, |
denotes |
, |
T7691 |
7431-7439 |
NN |
denotes |
analysis |
T7692 |
7440-7442 |
IN |
denotes |
of |
T7693 |
7443-7444 |
SYM |
denotes |
> |
T7694 |
7444-7446 |
CD |
denotes |
50 |
T7695 |
7447-7455 |
NNS |
denotes |
sections |
T7696 |
7456-7460 |
RB |
denotes |
each |
T7697 |
7460-7461 |
: |
denotes |
; |
T7698 |
7462-7468 |
NN |
denotes |
Figure |
T7677 |
7469-7471 |
NN |
denotes |
6F |
T7699 |
7472-7475 |
CC |
denotes |
and |
T7700 |
7476-7478 |
NN |
denotes |
6L |
T7701 |
7478-7479 |
-RRB- |
denotes |
) |
T7702 |
7479-7480 |
. |
denotes |
. |
T7703 |
7480-7759 |
sentence |
denotes |
Together with the in vitro culture experiments, these findings suggest that DRG neurons from TauEWS-Pea3/+ Isl1Cre/+ embryos remain post-mitotic but fail to become sensitive to naturally occurring cell death, and survive in the absence of Trk receptors and neurotrophic support. |
T7704 |
7481-7489 |
RB |
denotes |
Together |
T7706 |
7490-7494 |
IN |
denotes |
with |
T7707 |
7495-7498 |
DT |
denotes |
the |
T7709 |
7499-7501 |
FW |
denotes |
in |
T7710 |
7502-7507 |
FW |
denotes |
vitro |
T7711 |
7508-7515 |
NN |
denotes |
culture |
T7708 |
7516-7527 |
NNS |
denotes |
experiments |
T7712 |
7527-7529 |
, |
denotes |
, |
T7713 |
7529-7534 |
DT |
denotes |
these |
T7714 |
7535-7543 |
NNS |
denotes |
findings |
T7705 |
7544-7551 |
VBP |
denotes |
suggest |
T7715 |
7552-7556 |
IN |
denotes |
that |
T7717 |
7557-7560 |
NN |
denotes |
DRG |
T7718 |
7561-7568 |
NNS |
denotes |
neurons |
T7719 |
7569-7573 |
IN |
denotes |
from |
T7720 |
7574-7580 |
NN |
denotes |
TauEWS |
T7722 |
7580-7581 |
HYPH |
denotes |
- |
T7723 |
7581-7585 |
NN |
denotes |
Pea3 |
T7724 |
7585-7586 |
HYPH |
denotes |
/ |
T7725 |
7586-7587 |
SYM |
denotes |
+ |
T7721 |
7588-7595 |
NN |
denotes |
Isl1Cre |
T7727 |
7595-7596 |
HYPH |
denotes |
/ |
T7728 |
7596-7597 |
SYM |
denotes |
+ |
T7726 |
7598-7605 |
NNS |
denotes |
embryos |
T7716 |
7606-7612 |
VBP |
denotes |
remain |
T7729 |
7613-7625 |
JJ |
denotes |
post-mitotic |
T7730 |
7626-7629 |
CC |
denotes |
but |
T7731 |
7630-7634 |
VBP |
denotes |
fail |
T7732 |
7635-7637 |
TO |
denotes |
to |
T7733 |
7638-7644 |
VB |
denotes |
become |
T7734 |
7645-7654 |
JJ |
denotes |
sensitive |
T7735 |
7655-7657 |
IN |
denotes |
to |
T7736 |
7658-7667 |
RB |
denotes |
naturally |
T7737 |
7668-7677 |
VBG |
denotes |
occurring |
T7739 |
7678-7682 |
NN |
denotes |
cell |
T7738 |
7683-7688 |
NN |
denotes |
death |
T7740 |
7688-7690 |
, |
denotes |
, |
T7741 |
7690-7693 |
CC |
denotes |
and |
T7742 |
7694-7701 |
VBP |
denotes |
survive |
T7743 |
7702-7704 |
IN |
denotes |
in |
T7744 |
7705-7708 |
DT |
denotes |
the |
T7745 |
7709-7716 |
NN |
denotes |
absence |
T7746 |
7717-7719 |
IN |
denotes |
of |
T7747 |
7720-7723 |
NN |
denotes |
Trk |
T7748 |
7724-7733 |
NNS |
denotes |
receptors |
T7749 |
7734-7737 |
CC |
denotes |
and |
T7750 |
7738-7750 |
JJ |
denotes |
neurotrophic |
T7751 |
7751-7758 |
NN |
denotes |
support |
T7752 |
7758-7759 |
. |
denotes |
. |
T7753 |
7759-7957 |
sentence |
denotes |
We next analyzed whether changes in the expression of proteins known to be involved in the regulation of neuronal survival or cell death could be detected in DRG of TauEWS-Pea3/+ Isl1Cre/+ embryos. |
T7754 |
7760-7762 |
PRP |
denotes |
We |
T7756 |
7763-7767 |
RB |
denotes |
next |
T7755 |
7768-7776 |
VBD |
denotes |
analyzed |
T7757 |
7777-7784 |
IN |
denotes |
whether |
T7759 |
7785-7792 |
NNS |
denotes |
changes |
T7760 |
7793-7795 |
IN |
denotes |
in |
T7761 |
7796-7799 |
DT |
denotes |
the |
T7762 |
7800-7810 |
NN |
denotes |
expression |
T7763 |
7811-7813 |
IN |
denotes |
of |
T7764 |
7814-7822 |
NN |
denotes |
proteins |
T7765 |
7823-7828 |
VBN |
denotes |
known |
T7766 |
7829-7831 |
TO |
denotes |
to |
T7768 |
7832-7834 |
VB |
denotes |
be |
T7767 |
7835-7843 |
VBN |
denotes |
involved |
T7769 |
7844-7846 |
IN |
denotes |
in |
T7770 |
7847-7850 |
DT |
denotes |
the |
T7771 |
7851-7861 |
NN |
denotes |
regulation |
T7772 |
7862-7864 |
IN |
denotes |
of |
T7773 |
7865-7873 |
JJ |
denotes |
neuronal |
T7774 |
7874-7882 |
NN |
denotes |
survival |
T7775 |
7883-7885 |
CC |
denotes |
or |
T7776 |
7886-7890 |
NN |
denotes |
cell |
T7777 |
7891-7896 |
NN |
denotes |
death |
T7778 |
7897-7902 |
MD |
denotes |
could |
T7779 |
7903-7905 |
VB |
denotes |
be |
T7758 |
7906-7914 |
VBN |
denotes |
detected |
T7780 |
7915-7917 |
IN |
denotes |
in |
T7781 |
7918-7921 |
NN |
denotes |
DRG |
T7782 |
7922-7924 |
IN |
denotes |
of |
T7783 |
7925-7931 |
NN |
denotes |
TauEWS |
T7785 |
7931-7932 |
HYPH |
denotes |
- |
T7786 |
7932-7936 |
NN |
denotes |
Pea3 |
T7787 |
7936-7937 |
HYPH |
denotes |
/ |
T7788 |
7937-7938 |
SYM |
denotes |
+ |
T7784 |
7939-7946 |
NN |
denotes |
Isl1Cre |
T7790 |
7946-7947 |
HYPH |
denotes |
/ |
T7791 |
7947-7948 |
SYM |
denotes |
+ |
T7789 |
7949-7956 |
NNS |
denotes |
embryos |
T7792 |
7956-7957 |
. |
denotes |
. |
T7793 |
7957-8147 |
sentence |
denotes |
We found no significant quantitative changes in the level of Akt/p-Akt or CREB/p-CREB in DRG (Figure 6O and 6P) both of which have been shown to be key regulators of neuronal survival [29]. |
T7794 |
7958-7960 |
PRP |
denotes |
We |
T7795 |
7961-7966 |
VBD |
denotes |
found |
T7796 |
7967-7969 |
DT |
denotes |
no |
T7798 |
7970-7981 |
JJ |
denotes |
significant |
T7799 |
7982-7994 |
JJ |
denotes |
quantitative |
T7797 |
7995-8002 |
NNS |
denotes |
changes |
T7800 |
8003-8005 |
IN |
denotes |
in |
T7801 |
8006-8009 |
DT |
denotes |
the |
T7802 |
8010-8015 |
NN |
denotes |
level |
T7803 |
8016-8018 |
IN |
denotes |
of |
T7804 |
8019-8022 |
NN |
denotes |
Akt |
T7806 |
8022-8023 |
HYPH |
denotes |
/ |
T7807 |
8023-8024 |
NN |
denotes |
p |
T7808 |
8024-8025 |
HYPH |
denotes |
- |
T7805 |
8025-8028 |
NN |
denotes |
Akt |
T7809 |
8029-8031 |
CC |
denotes |
or |
T7810 |
8032-8036 |
NN |
denotes |
CREB |
T7812 |
8036-8037 |
HYPH |
denotes |
/ |
T7813 |
8037-8038 |
NN |
denotes |
p |
T7814 |
8038-8039 |
HYPH |
denotes |
- |
T7811 |
8039-8043 |
NN |
denotes |
CREB |
T7815 |
8044-8046 |
IN |
denotes |
in |
T7816 |
8047-8050 |
NN |
denotes |
DRG |
T7817 |
8051-8052 |
-LRB- |
denotes |
( |
T7819 |
8052-8058 |
NN |
denotes |
Figure |
T7818 |
8059-8061 |
NN |
denotes |
6O |
T7820 |
8062-8065 |
CC |
denotes |
and |
T7821 |
8066-8068 |
NN |
denotes |
6P |
T7822 |
8068-8069 |
-RRB- |
denotes |
) |
T7823 |
8070-8074 |
DT |
denotes |
both |
T7825 |
8075-8077 |
IN |
denotes |
of |
T7826 |
8078-8083 |
WDT |
denotes |
which |
T7827 |
8084-8088 |
VBP |
denotes |
have |
T7828 |
8089-8093 |
VBN |
denotes |
been |
T7824 |
8094-8099 |
VBN |
denotes |
shown |
T7829 |
8100-8102 |
TO |
denotes |
to |
T7830 |
8103-8105 |
VB |
denotes |
be |
T7831 |
8106-8109 |
JJ |
denotes |
key |
T7832 |
8110-8120 |
NNS |
denotes |
regulators |
T7833 |
8121-8123 |
IN |
denotes |
of |
T7834 |
8124-8132 |
JJ |
denotes |
neuronal |
T7835 |
8133-8141 |
NN |
denotes |
survival |
T7836 |
8142-8143 |
-LRB- |
denotes |
[ |
T7837 |
8143-8145 |
CD |
denotes |
29 |
T7838 |
8145-8146 |
-RRB- |
denotes |
] |
T7839 |
8146-8147 |
. |
denotes |
. |
T7840 |
8147-8260 |
sentence |
denotes |
Moreover, the level of the proapoptotic Bcl2 family member Bax was not significantly reduced (Figure 6O and 6P). |
T7841 |
8148-8156 |
RB |
denotes |
Moreover |
T7843 |
8156-8158 |
, |
denotes |
, |
T7844 |
8158-8161 |
DT |
denotes |
the |
T7845 |
8162-8167 |
NN |
denotes |
level |
T7846 |
8168-8170 |
IN |
denotes |
of |
T7847 |
8171-8174 |
DT |
denotes |
the |
T7849 |
8175-8187 |
JJ |
denotes |
proapoptotic |
T7850 |
8188-8192 |
NN |
denotes |
Bcl2 |
T7851 |
8193-8199 |
NN |
denotes |
family |
T7852 |
8200-8206 |
NN |
denotes |
member |
T7848 |
8207-8210 |
NN |
denotes |
Bax |
T7853 |
8211-8214 |
VBD |
denotes |
was |
T7854 |
8215-8218 |
RB |
denotes |
not |
T7855 |
8219-8232 |
RB |
denotes |
significantly |
T7842 |
8233-8240 |
VBN |
denotes |
reduced |
T7856 |
8241-8242 |
-LRB- |
denotes |
( |
T7858 |
8242-8248 |
NN |
denotes |
Figure |
T7857 |
8249-8251 |
NN |
denotes |
6O |
T7859 |
8252-8255 |
CC |
denotes |
and |
T7860 |
8256-8258 |
NN |
denotes |
6P |
T7861 |
8258-8259 |
-RRB- |
denotes |
) |
T7862 |
8259-8260 |
. |
denotes |
. |
T7863 |
8260-8679 |
sentence |
denotes |
In contrast, the expression level of the anti-apoptotic Bcl2 family members Bcl-xl and Bcl2 was significantly increased when compared to wild-type levels (Bcl2, 157%; Bcl-xl, 259%; average of n = 3 independent experiments; Figure 6O and 6P), providing a potential molecular explanation for the enhanced neuronal survival of DRG neurons of TauEWS-Pea3/+ Isl1Cre/+ embryos in the absence of Trk receptor expression [30]. |
T7864 |
8261-8263 |
IN |
denotes |
In |
T7866 |
8264-8272 |
NN |
denotes |
contrast |
T7867 |
8272-8274 |
, |
denotes |
, |
T7868 |
8274-8277 |
DT |
denotes |
the |
T7870 |
8278-8288 |
NN |
denotes |
expression |
T7869 |
8289-8294 |
NN |
denotes |
level |
T7871 |
8295-8297 |
IN |
denotes |
of |
T7872 |
8298-8301 |
DT |
denotes |
the |
T7874 |
8302-8316 |
JJ |
denotes |
anti-apoptotic |
T7875 |
8317-8321 |
NN |
denotes |
Bcl2 |
T7876 |
8322-8328 |
NN |
denotes |
family |
T7873 |
8329-8336 |
NNS |
denotes |
members |
T7877 |
8337-8340 |
NN |
denotes |
Bcl |
T7879 |
8340-8341 |
HYPH |
denotes |
- |
T7878 |
8341-8343 |
NN |
denotes |
xl |
T7880 |
8344-8347 |
CC |
denotes |
and |
T7881 |
8348-8352 |
NN |
denotes |
Bcl2 |
T7882 |
8353-8356 |
VBD |
denotes |
was |
T7883 |
8357-8370 |
RB |
denotes |
significantly |
T7865 |
8371-8380 |
VBN |
denotes |
increased |
T7884 |
8381-8385 |
WRB |
denotes |
when |
T7885 |
8386-8394 |
VBN |
denotes |
compared |
T7886 |
8395-8397 |
IN |
denotes |
to |
T7887 |
8398-8402 |
JJ |
denotes |
wild |
T7889 |
8402-8403 |
HYPH |
denotes |
- |
T7888 |
8403-8407 |
NN |
denotes |
type |
T7890 |
8408-8414 |
NNS |
denotes |
levels |
T7891 |
8415-8416 |
-LRB- |
denotes |
( |
T7893 |
8416-8420 |
NN |
denotes |
Bcl2 |
T7895 |
8420-8422 |
, |
denotes |
, |
T7896 |
8422-8425 |
CD |
denotes |
157 |
T7894 |
8425-8426 |
NN |
denotes |
% |
T7897 |
8426-8427 |
: |
denotes |
; |
T7898 |
8428-8431 |
NN |
denotes |
Bcl |
T7900 |
8431-8432 |
HYPH |
denotes |
- |
T7899 |
8432-8434 |
NN |
denotes |
xl |
T7902 |
8434-8436 |
, |
denotes |
, |
T7903 |
8436-8439 |
CD |
denotes |
259 |
T7901 |
8439-8440 |
NN |
denotes |
% |
T7904 |
8440-8441 |
: |
denotes |
; |
T7905 |
8442-8449 |
NN |
denotes |
average |
T7906 |
8450-8452 |
IN |
denotes |
of |
T7907 |
8453-8454 |
NN |
denotes |
n |
T7909 |
8455-8456 |
SYM |
denotes |
= |
T7910 |
8457-8458 |
CD |
denotes |
3 |
T7911 |
8459-8470 |
JJ |
denotes |
independent |
T7908 |
8471-8482 |
NNS |
denotes |
experiments |
T7912 |
8482-8483 |
: |
denotes |
; |
T7913 |
8484-8490 |
NN |
denotes |
Figure |
T7892 |
8491-8493 |
NN |
denotes |
6O |
T7914 |
8494-8497 |
CC |
denotes |
and |
T7915 |
8498-8500 |
NN |
denotes |
6P |
T7916 |
8500-8501 |
-RRB- |
denotes |
) |
T7917 |
8501-8503 |
, |
denotes |
, |
T7918 |
8503-8512 |
VBG |
denotes |
providing |
T7919 |
8513-8514 |
DT |
denotes |
a |
T7921 |
8515-8524 |
JJ |
denotes |
potential |
T7922 |
8525-8534 |
JJ |
denotes |
molecular |
T7920 |
8535-8546 |
NN |
denotes |
explanation |
T7923 |
8547-8550 |
IN |
denotes |
for |
T7924 |
8551-8554 |
DT |
denotes |
the |
T7926 |
8555-8563 |
VBN |
denotes |
enhanced |
T7927 |
8564-8572 |
JJ |
denotes |
neuronal |
T7925 |
8573-8581 |
NN |
denotes |
survival |
T7928 |
8582-8584 |
IN |
denotes |
of |
T7929 |
8585-8588 |
NN |
denotes |
DRG |
T7930 |
8589-8596 |
NNS |
denotes |
neurons |
T7931 |
8597-8599 |
IN |
denotes |
of |
T7932 |
8600-8606 |
NN |
denotes |
TauEWS |
T7934 |
8606-8607 |
HYPH |
denotes |
- |
T7935 |
8607-8611 |
NN |
denotes |
Pea3 |
T7936 |
8611-8612 |
HYPH |
denotes |
/ |
T7937 |
8612-8613 |
SYM |
denotes |
+ |
T7933 |
8614-8621 |
NN |
denotes |
Isl1Cre |
T7939 |
8621-8622 |
HYPH |
denotes |
/ |
T7940 |
8622-8623 |
SYM |
denotes |
+ |
T7938 |
8624-8631 |
NNS |
denotes |
embryos |
T7941 |
8632-8634 |
IN |
denotes |
in |
T7942 |
8635-8638 |
DT |
denotes |
the |
T7943 |
8639-8646 |
NN |
denotes |
absence |
T7944 |
8647-8649 |
IN |
denotes |
of |
T7945 |
8650-8653 |
NN |
denotes |
Trk |
T7946 |
8654-8662 |
NN |
denotes |
receptor |
T7947 |
8663-8673 |
NN |
denotes |
expression |
T7948 |
8674-8675 |
-LRB- |
denotes |
[ |
T7949 |
8675-8677 |
CD |
denotes |
30 |
T7950 |
8677-8678 |
-RRB- |
denotes |
] |
T7951 |
8678-8679 |
. |
denotes |
. |
R2451 |
T6663 |
T6664 |
amod |
Precocious,Expression |
R2452 |
T6664 |
T6668 |
nsubj |
Expression,Promotes |
R2453 |
T6665 |
T6666 |
compound |
EWS,Pea3 |
R2454 |
T6666 |
T6664 |
compound |
Pea3,Expression |
R2455 |
T6667 |
T6666 |
punct |
-,Pea3 |
R2456 |
T6669 |
T6670 |
npadvmod |
Neurotrophin,Independent |
R2457 |
T6670 |
T6672 |
amod |
Independent,Survival |
R2458 |
T6671 |
T6670 |
punct |
-,Independent |
R2459 |
T6672 |
T6668 |
dobj |
Survival,Promotes |
R2460 |
T6673 |
T6672 |
cc |
and,Survival |
R2461 |
T6674 |
T6675 |
compound |
Neurite,Outgrowth |
R2462 |
T6675 |
T6672 |
conj |
Outgrowth,Survival |
R2463 |
T6677 |
T6678 |
aux |
To,begin |
R2464 |
T6678 |
T6679 |
advcl |
begin,turned |
R2465 |
T6680 |
T6681 |
aux |
to,address |
R2466 |
T6681 |
T6678 |
xcomp |
address,begin |
R2467 |
T6682 |
T6683 |
det |
the,mechanisms |
R2468 |
T6683 |
T6681 |
dobj |
mechanisms,address |
R2469 |
T6684 |
T6683 |
amod |
cellular,mechanisms |
R2470 |
T6685 |
T6684 |
cc |
and,cellular |
R2471 |
T6686 |
T6684 |
conj |
molecular,cellular |
R2472 |
T6687 |
T6683 |
acl |
involved,mechanisms |
R2473 |
T6688 |
T6687 |
prep |
in,involved |
R2474 |
T6689 |
T6690 |
det |
the,actions |
R2475 |
T6690 |
T6688 |
pobj |
actions,in |
R2476 |
T6691 |
T6690 |
amod |
distinct,actions |
R2477 |
T6692 |
T6690 |
amod |
biological,actions |
R2478 |
T6693 |
T6690 |
prep |
of,actions |
R2479 |
T6694 |
T6695 |
compound |
EWS,Pea3 |
R2480 |
T6695 |
T6693 |
pobj |
Pea3,of |
R2481 |
T6696 |
T6695 |
punct |
-,Pea3 |
R2482 |
T6697 |
T6687 |
prep |
at,involved |
R2483 |
T6698 |
T6699 |
amod |
different,stages |
R2484 |
T6699 |
T6697 |
pobj |
stages,at |
R2485 |
T6700 |
T6699 |
amod |
developmental,stages |
R2486 |
T6701 |
T6679 |
punct |
", ",turned |
R2487 |
T6702 |
T6679 |
nsubj |
we,turned |
R2488 |
T6703 |
T6679 |
advmod |
first,turned |
R2489 |
T6704 |
T6679 |
prep |
to,turned |
R2490 |
T6705 |
T6706 |
advmod |
in,vitro |
R2491 |
T6706 |
T6707 |
amod |
vitro,experiments |
R2492 |
T6707 |
T6704 |
pobj |
experiments,to |
R2493 |
T6708 |
T6707 |
compound |
culture,experiments |
R2494 |
T6709 |
T6679 |
punct |
.,turned |
R2495 |
T6711 |
T6712 |
det |
These,experiments |
R2496 |
T6712 |
T6713 |
nsubj |
experiments,permit |
R2497 |
T6714 |
T6713 |
dobj |
assessment,permit |
R2498 |
T6715 |
T6714 |
prep |
of,assessment |
R2499 |
T6716 |
T6717 |
mark |
whether,influences |
R2500 |
T6717 |
T6715 |
pcomp |
influences,of |
R2501 |
T6718 |
T6719 |
amod |
precocious,signaling |
R2502 |
T6719 |
T6717 |
nsubj |
signaling,influences |
R2503 |
T6720 |
T6719 |
compound |
ETS,signaling |
R2504 |
T6721 |
T6722 |
compound |
transcription,factor |
R2505 |
T6722 |
T6719 |
compound |
factor,signaling |
R2506 |
T6723 |
T6724 |
amod |
neuronal,survival |
R2507 |
T6724 |
T6717 |
dobj |
survival,influences |
R2508 |
T6725 |
T6724 |
cc |
and,survival |
R2509 |
T6726 |
T6727 |
nmod |
in,vitro |
R2510 |
T6727 |
T6728 |
nmod |
vitro,outgrowth |
R2511 |
T6728 |
T6724 |
conj |
outgrowth,survival |
R2512 |
T6729 |
T6728 |
compound |
neurite,outgrowth |
R2513 |
T6730 |
T6724 |
prep |
of,survival |
R2514 |
T6731 |
T6732 |
compound |
DRG,neurons |
R2515 |
T6732 |
T6730 |
pobj |
neurons,of |
R2516 |
T6733 |
T6724 |
punct |
", ",survival |
R2517 |
T6734 |
T6735 |
nummod |
two,parameters |
R2518 |
T6735 |
T6724 |
appos |
parameters,survival |
R2519 |
T6736 |
T6737 |
advmod |
prominently,influenced |
R2520 |
T6737 |
T6735 |
acl |
influenced,parameters |
R2521 |
T6738 |
T6737 |
agent |
by,influenced |
R2522 |
T6739 |
T6740 |
npadvmod |
target,derived |
R2523 |
T6740 |
T6742 |
amod |
derived,factors |
R2524 |
T6741 |
T6740 |
punct |
-,derived |
R2525 |
T6742 |
T6738 |
pobj |
factors,by |
R2526 |
T6743 |
T6742 |
amod |
neurotrophic,factors |
R2527 |
T6744 |
T6742 |
cc |
and,factors |
R2528 |
T6745 |
T6746 |
poss |
their,receptors |
R2529 |
T6746 |
T6742 |
conj |
receptors,factors |
R2530 |
T6747 |
T6713 |
punct |
.,permit |
R2531 |
T6749 |
T6750 |
nsubj |
We,cultured |
R2532 |
T6751 |
T6752 |
nmod |
E13.5,explants |
R2533 |
T6752 |
T6750 |
dobj |
explants,cultured |
R2534 |
T6753 |
T6752 |
amod |
whole,explants |
R2535 |
T6754 |
T6752 |
compound |
DRG,explants |
R2536 |
T6755 |
T6752 |
prep |
from,explants |
R2537 |
T6756 |
T6757 |
amod |
wild,type |
R2538 |
T6757 |
T6759 |
nmod |
type,embryos |
R2539 |
T6758 |
T6757 |
punct |
-,type |
R2540 |
T6759 |
T6755 |
pobj |
embryos,from |
R2541 |
T6760 |
T6757 |
cc |
and,type |
R2542 |
T6761 |
T6762 |
nmod |
TauEWS,Isl1Cre |
R2543 |
T6762 |
T6757 |
conj |
Isl1Cre,type |
R2544 |
T6763 |
T6762 |
punct |
-,Isl1Cre |
R2545 |
T6764 |
T6762 |
nmod |
Pea3,Isl1Cre |
R2546 |
T6765 |
T6762 |
punct |
/,Isl1Cre |
R2547 |
T6766 |
T6762 |
punct |
+,Isl1Cre |
R2548 |
T6767 |
T6762 |
punct |
/,Isl1Cre |
R2549 |
T6768 |
T6762 |
punct |
+,Isl1Cre |
R2550 |
T6769 |
T6752 |
prep |
in,explants |
R2551 |
T6770 |
T6771 |
det |
the,presence |
R2552 |
T6771 |
T6769 |
pobj |
presence,in |
R2553 |
T6772 |
T6771 |
prep |
of,presence |
R2554 |
T6773 |
T6772 |
pobj |
NGF,of |
R2555 |
T6774 |
T6773 |
cc |
or,NGF |
R2556 |
T6775 |
T6773 |
conj |
NT,NGF |
R2557 |
T6776 |
T6775 |
punct |
-,NT |
R2558 |
T6777 |
T6775 |
nummod |
3,NT |
R2559 |
T6778 |
T6769 |
cc |
or,in |
R2560 |
T6779 |
T6769 |
conj |
in,in |
R2561 |
T6780 |
T6781 |
det |
the,absence |
R2562 |
T6781 |
T6779 |
pobj |
absence,in |
R2563 |
T6782 |
T6781 |
prep |
of,absence |
R2564 |
T6783 |
T6782 |
pobj |
neurotrophins,of |
R2565 |
T6784 |
T6750 |
cc |
and,cultured |
R2566 |
T6785 |
T6750 |
conj |
analyzed,cultured |
R2567 |
T6786 |
T6787 |
amod |
neuronal,survival |
R2568 |
T6787 |
T6785 |
dobj |
survival,analyzed |
R2569 |
T6788 |
T6787 |
cc |
and,survival |
R2570 |
T6789 |
T6790 |
compound |
neurite,outgrowth |
R2571 |
T6790 |
T6787 |
conj |
outgrowth,survival |
R2572 |
T6791 |
T6785 |
prep |
on,analyzed |
R2573 |
T6792 |
T6793 |
compound |
matrigel,substrate |
R2574 |
T6793 |
T6791 |
pobj |
substrate,on |
R2575 |
T6794 |
T6785 |
prep |
after,analyzed |
R2576 |
T6795 |
T6796 |
nummod |
48,h |
R2577 |
T6796 |
T6794 |
pobj |
h,after |
R2578 |
T6797 |
T6798 |
advmod |
in,vitro |
R2579 |
T6798 |
T6785 |
advmod |
vitro,analyzed |
R2580 |
T6799 |
T6750 |
punct |
.,cultured |
R2581 |
T6801 |
T6802 |
prep |
Without,survived |
R2582 |
T6803 |
T6804 |
amod |
neurotrophic,support |
R2583 |
T6804 |
T6801 |
pobj |
support,Without |
R2584 |
T6805 |
T6802 |
punct |
", ",survived |
R2585 |
T6806 |
T6807 |
advmod |
very,few |
R2586 |
T6807 |
T6808 |
amod |
few,neurons |
R2587 |
T6808 |
T6802 |
nsubj |
neurons,survived |
R2588 |
T6809 |
T6810 |
amod |
wild,type |
R2589 |
T6810 |
T6808 |
compound |
type,neurons |
R2590 |
T6811 |
T6810 |
punct |
-,type |
R2591 |
T6812 |
T6808 |
compound |
DRG,neurons |
R2592 |
T6813 |
T6814 |
punct |
(,4A |
R2593 |
T6814 |
T6802 |
parataxis |
4A,survived |
R2594 |
T6815 |
T6814 |
compound |
Figure,4A |
R2595 |
T6816 |
T6814 |
punct |
),4A |
R2596 |
T6817 |
T6802 |
punct |
.,survived |
R2597 |
T6819 |
T6820 |
prep |
In,led |
R2598 |
T6821 |
T6819 |
pobj |
contrast,In |
R2599 |
T6822 |
T6820 |
punct |
", ",led |
R2600 |
T6823 |
T6820 |
csubj |
culturing,led |
R2601 |
T6824 |
T6825 |
amod |
wild,type |
R2602 |
T6825 |
T6827 |
compound |
type,DRG |
R2603 |
T6826 |
T6825 |
punct |
-,type |
R2604 |
T6827 |
T6823 |
dobj |
DRG,culturing |
R2605 |
T6828 |
T6823 |
prep |
with,culturing |
R2606 |
T6829 |
T6830 |
amod |
neurotrophic,factors |
R2607 |
T6830 |
T6828 |
pobj |
factors,with |
R2608 |
T6831 |
T6820 |
prep |
to,led |
R2609 |
T6832 |
T6833 |
amod |
neuronal,survival |
R2610 |
T6833 |
T6831 |
pobj |
survival,to |
R2611 |
T6834 |
T6833 |
cc |
and,survival |
R2612 |
T6835 |
T6836 |
compound |
neurite,outgrowth |
R2613 |
T6836 |
T6833 |
conj |
outgrowth,survival |
R2614 |
T6837 |
T6820 |
punct |
.,led |
R2615 |
T6839 |
T6840 |
nsubj |
Addition,resulted |
R2616 |
T6841 |
T6839 |
prep |
of,Addition |
R2617 |
T6842 |
T6841 |
pobj |
NGF,of |
R2618 |
T6843 |
T6842 |
punct |
", ",NGF |
R2619 |
T6844 |
T6845 |
dep |
which,supports |
R2620 |
T6845 |
T6842 |
relcl |
supports,NGF |
R2621 |
T6846 |
T6845 |
dobj |
survival,supports |
R2622 |
T6847 |
T6846 |
prep |
of,survival |
R2623 |
T6848 |
T6849 |
amod |
cutaneous,afferents |
R2624 |
T6849 |
T6847 |
pobj |
afferents,of |
R2625 |
T6850 |
T6840 |
punct |
", ",resulted |
R2626 |
T6851 |
T6840 |
prep |
in,resulted |
R2627 |
T6852 |
T6853 |
amod |
straight,outgrowth |
R2628 |
T6853 |
T6851 |
pobj |
outgrowth,in |
R2629 |
T6854 |
T6852 |
cc |
and,straight |
R2630 |
T6855 |
T6852 |
conj |
unbranched,straight |
R2631 |
T6856 |
T6853 |
compound |
neurite,outgrowth |
R2632 |
T6857 |
T6858 |
punct |
(,4B |
R2633 |
T6858 |
T6853 |
parataxis |
4B,outgrowth |
R2634 |
T6859 |
T6858 |
compound |
Figure,4B |
R2635 |
T6860 |
T6858 |
punct |
),4B |
R2636 |
T6861 |
T6840 |
punct |
", ",resulted |
R2637 |
T6862 |
T6863 |
mark |
while,resulted |
R2638 |
T6863 |
T6840 |
advcl |
resulted,resulted |
R2639 |
T6864 |
T6863 |
nsubj |
cultures,resulted |
R2640 |
T6865 |
T6864 |
acl |
grown,cultures |
R2641 |
T6866 |
T6865 |
prep |
in,grown |
R2642 |
T6867 |
T6868 |
det |
the,presence |
R2643 |
T6868 |
T6866 |
pobj |
presence,in |
R2644 |
T6869 |
T6868 |
prep |
of,presence |
R2645 |
T6870 |
T6869 |
pobj |
NT,of |
R2646 |
T6871 |
T6870 |
punct |
-,NT |
R2647 |
T6872 |
T6870 |
nummod |
3,NT |
R2648 |
T6873 |
T6870 |
punct |
", ",NT |
R2649 |
T6874 |
T6875 |
dep |
which,supports |
R2650 |
T6875 |
T6870 |
relcl |
supports,NT |
R2651 |
T6876 |
T6875 |
dobj |
survival,supports |
R2652 |
T6877 |
T6876 |
prep |
of,survival |
R2653 |
T6878 |
T6879 |
amod |
proprioceptive,afferents |
R2654 |
T6879 |
T6877 |
pobj |
afferents,of |
R2655 |
T6880 |
T6863 |
punct |
", ",resulted |
R2656 |
T6881 |
T6863 |
prep |
in,resulted |
R2657 |
T6882 |
T6883 |
det |
a,pattern |
R2658 |
T6883 |
T6881 |
pobj |
pattern,in |
R2659 |
T6884 |
T6885 |
advmod |
highly,branched |
R2660 |
T6885 |
T6883 |
amod |
branched,pattern |
R2661 |
T6886 |
T6887 |
compound |
neurite,outgrowth |
R2662 |
T6887 |
T6883 |
compound |
outgrowth,pattern |
R2663 |
T6888 |
T6863 |
prep |
after,resulted |
R2664 |
T6889 |
T6890 |
nummod |
48,h |
R2665 |
T6890 |
T6888 |
pobj |
h,after |
R2666 |
T6891 |
T6892 |
advmod |
in,vitro |
R2667 |
T6892 |
T6890 |
advmod |
vitro,h |
R2668 |
T6893 |
T6894 |
punct |
(,4C |
R2669 |
T6894 |
T6863 |
parataxis |
4C,resulted |
R2670 |
T6895 |
T6894 |
compound |
Figure,4C |
R2671 |
T6896 |
T6894 |
punct |
),4C |
R2672 |
T6897 |
T6840 |
punct |
.,resulted |
R2673 |
T6899 |
T6900 |
advmod |
Surprisingly,survived |
R2674 |
T6901 |
T6900 |
punct |
", ",survived |
R2675 |
T6902 |
T6903 |
compound |
DRG,neurons |
R2676 |
T6903 |
T6900 |
nsubj |
neurons,survived |
R2677 |
T6904 |
T6903 |
acl |
isolated,neurons |
R2678 |
T6905 |
T6904 |
prep |
from,isolated |
R2679 |
T6906 |
T6907 |
nmod |
TauEWS,Isl1Cre |
R2680 |
T6907 |
T6912 |
nmod |
Isl1Cre,embryos |
R2681 |
T6908 |
T6907 |
punct |
-,Isl1Cre |
R2682 |
T6909 |
T6907 |
nmod |
Pea3,Isl1Cre |
R2683 |
T6910 |
T6907 |
punct |
/,Isl1Cre |
R2684 |
T6911 |
T6907 |
punct |
+,Isl1Cre |
R2685 |
T6912 |
T6905 |
pobj |
embryos,from |
R2686 |
T6913 |
T6907 |
punct |
/,Isl1Cre |
R2687 |
T6914 |
T6907 |
punct |
+,Isl1Cre |
R2688 |
T6915 |
T6904 |
cc |
and,isolated |
R2689 |
T6916 |
T6904 |
conj |
cultured,isolated |
R2690 |
T6917 |
T6916 |
prep |
without,cultured |
R2691 |
T6918 |
T6919 |
amod |
neurotrophic,support |
R2692 |
T6919 |
T6917 |
pobj |
support,without |
R2693 |
T6920 |
T6900 |
prep |
after,survived |
R2694 |
T6921 |
T6922 |
nummod |
48,h |
R2695 |
T6922 |
T6920 |
pobj |
h,after |
R2696 |
T6923 |
T6924 |
advmod |
in,vitro |
R2697 |
T6924 |
T6922 |
advmod |
vitro,h |
R2698 |
T6925 |
T6900 |
cc |
and,survived |
R2699 |
T6926 |
T6927 |
aux |
had,established |
R2700 |
T6927 |
T6900 |
conj |
established,survived |
R2701 |
T6928 |
T6929 |
amod |
long,neurites |
R2702 |
T6929 |
T6927 |
dobj |
neurites,established |
R2703 |
T6930 |
T6928 |
cc |
and,long |
R2704 |
T6931 |
T6932 |
advmod |
highly,branched |
R2705 |
T6932 |
T6928 |
conj |
branched,long |
R2706 |
T6933 |
T6934 |
punct |
(,4D |
R2707 |
T6934 |
T6927 |
parataxis |
4D,established |
R2708 |
T6935 |
T6934 |
compound |
Figure,4D |
R2709 |
T6936 |
T6934 |
punct |
),4D |
R2710 |
T6937 |
T6900 |
punct |
.,survived |
R2711 |
T6939 |
T6940 |
preconj |
Neither,pattern |
R2712 |
T6940 |
T6942 |
nsubj |
pattern,changed |
R2713 |
T6941 |
T6940 |
det |
the,pattern |
R2714 |
T6943 |
T6940 |
prep |
of,pattern |
R2715 |
T6944 |
T6945 |
compound |
neurite,outgrowth |
R2716 |
T6945 |
T6943 |
pobj |
outgrowth,of |
R2717 |
T6946 |
T6940 |
cc |
nor,pattern |
R2718 |
T6947 |
T6948 |
amod |
neuronal,survival |
R2719 |
T6948 |
T6940 |
conj |
survival,pattern |
R2720 |
T6949 |
T6942 |
advmod |
significantly,changed |
R2721 |
T6950 |
T6942 |
prep |
after,changed |
R2722 |
T6951 |
T6950 |
pobj |
application,after |
R2723 |
T6952 |
T6951 |
prep |
of,application |
R2724 |
T6953 |
T6954 |
preconj |
either,NGF |
R2725 |
T6954 |
T6952 |
pobj |
NGF,of |
R2726 |
T6955 |
T6954 |
cc |
or,NGF |
R2727 |
T6956 |
T6954 |
conj |
NT,NGF |
R2728 |
T6957 |
T6956 |
punct |
-,NT |
R2729 |
T6958 |
T6956 |
nummod |
3,NT |
R2730 |
T6959 |
T6960 |
punct |
(,4E |
R2731 |
T6960 |
T6942 |
parataxis |
4E,changed |
R2732 |
T6961 |
T6960 |
compound |
Figure,4E |
R2733 |
T6962 |
T6960 |
cc |
and,4E |
R2734 |
T6963 |
T6960 |
conj |
4F,4E |
R2735 |
T6964 |
T6960 |
punct |
),4E |
R2736 |
T6965 |
T6942 |
punct |
.,changed |
R2737 |
T6967 |
T6968 |
aux |
To,compare |
R2738 |
T6968 |
T6970 |
advcl |
compare,generated |
R2739 |
T6969 |
T6968 |
advmod |
directly,compare |
R2740 |
T6971 |
T6972 |
compound |
neurotrophin,dependence |
R2741 |
T6972 |
T6968 |
dobj |
dependence,compare |
R2742 |
T6973 |
T6972 |
prep |
of,dependence |
R2743 |
T6974 |
T6975 |
compound |
DRG,neurons |
R2744 |
T6975 |
T6973 |
pobj |
neurons,of |
R2745 |
T6976 |
T6975 |
acl |
expressing,neurons |
R2746 |
T6977 |
T6978 |
compound |
EWS,Pea3 |
R2747 |
T6978 |
T6976 |
dobj |
Pea3,expressing |
R2748 |
T6979 |
T6978 |
punct |
-,Pea3 |
R2749 |
T6980 |
T6978 |
prep |
from,Pea3 |
R2750 |
T6981 |
T6982 |
det |
the,locus |
R2751 |
T6982 |
T6980 |
pobj |
locus,from |
R2752 |
T6983 |
T6982 |
compound |
Tau,locus |
R2753 |
T6984 |
T6982 |
prep |
at,locus |
R2754 |
T6985 |
T6986 |
det |
a,time |
R2755 |
T6986 |
T6984 |
pobj |
time,at |
R2756 |
T6987 |
T6986 |
amod |
precocious,time |
R2757 |
T6988 |
T6987 |
cc |
versus,precocious |
R2758 |
T6989 |
T6987 |
conj |
isochronic,precocious |
R2759 |
T6990 |
T6986 |
prep |
of,time |
R2760 |
T6991 |
T6990 |
pobj |
onset,of |
R2761 |
T6992 |
T6970 |
punct |
", ",generated |
R2762 |
T6993 |
T6970 |
nsubj |
we,generated |
R2763 |
T6994 |
T6995 |
det |
a,strain |
R2764 |
T6995 |
T6970 |
dobj |
strain,generated |
R2765 |
T6996 |
T6995 |
prep |
of,strain |
R2766 |
T6997 |
T6996 |
pobj |
mice,of |
R2767 |
T6998 |
T6999 |
prep |
in,expressed |
R2768 |
T6999 |
T6995 |
relcl |
expressed,strain |
R2769 |
T7000 |
T6998 |
pobj |
which,in |
R2770 |
T7001 |
T7002 |
compound |
Cre,recombinase |
R2771 |
T7002 |
T6999 |
nsubjpass |
recombinase,expressed |
R2772 |
T7003 |
T6999 |
auxpass |
is,expressed |
R2773 |
T7004 |
T6999 |
prep |
from,expressed |
R2774 |
T7005 |
T7006 |
det |
the,locus |
R2775 |
T7006 |
T7004 |
pobj |
locus,from |
R2776 |
T7007 |
T7006 |
compound |
PV,locus |
R2777 |
T7008 |
T7009 |
punct |
(,S4 |
R2778 |
T7009 |
T6970 |
parataxis |
S4,generated |
R2779 |
T7010 |
T7009 |
compound |
Figure,S4 |
R2780 |
T7011 |
T7009 |
punct |
),S4 |
R2781 |
T7012 |
T6970 |
punct |
.,generated |
R2782 |
T7014 |
T7015 |
det |
The,expression |
R2783 |
T7015 |
T7016 |
nsubjpass |
expression,restricted |
R2784 |
T7017 |
T7015 |
prep |
of,expression |
R2785 |
T7018 |
T7017 |
pobj |
GFP,of |
R2786 |
T7019 |
T7015 |
prep |
in,expression |
R2787 |
T7020 |
T7021 |
nmod |
TaumGFP,PVCre |
R2788 |
T7021 |
T7019 |
pobj |
PVCre,in |
R2789 |
T7022 |
T7021 |
punct |
/,PVCre |
R2790 |
T7023 |
T7021 |
punct |
+,PVCre |
R2791 |
T7024 |
T7021 |
punct |
/,PVCre |
R2792 |
T7025 |
T7021 |
punct |
+,PVCre |
R2793 |
T7026 |
T7016 |
auxpass |
was,restricted |
R2794 |
T7027 |
T7016 |
prep |
to,restricted |
R2795 |
T7028 |
T7029 |
nmod |
PV,neurons |
R2796 |
T7029 |
T7027 |
pobj |
neurons,to |
R2797 |
T7030 |
T7028 |
punct |
+,PV |
R2798 |
T7031 |
T7029 |
amod |
proprioceptive,neurons |
R2799 |
T7032 |
T7029 |
compound |
DRG,neurons |
R2800 |
T7033 |
T7016 |
cc |
and,restricted |
R2801 |
T7034 |
T7016 |
conj |
mirrored,restricted |
R2802 |
T7035 |
T7036 |
det |
the,onset |
R2803 |
T7036 |
T7034 |
dobj |
onset,mirrored |
R2804 |
T7037 |
T7036 |
prep |
of,onset |
R2805 |
T7038 |
T7037 |
pobj |
expression,of |
R2806 |
T7039 |
T7038 |
prep |
of,expression |
R2807 |
T7040 |
T7039 |
pobj |
PV,of |
R2808 |
T7041 |
T7034 |
prep |
at,mirrored |
R2809 |
T7042 |
T7043 |
advmod |
approximately,E14 |
R2810 |
T7043 |
T7041 |
pobj |
E14,at |
R2811 |
T7044 |
T7045 |
punct |
(,S4 |
R2812 |
T7045 |
T7043 |
parataxis |
S4,E14 |
R2813 |
T7046 |
T7045 |
compound |
Figure,S4 |
R2814 |
T7047 |
T7045 |
punct |
;,S4 |
R2815 |
T7048 |
T7049 |
nsubj |
data,shown |
R2816 |
T7049 |
T7045 |
ccomp |
shown,S4 |
R2817 |
T7050 |
T7049 |
neg |
not,shown |
R2818 |
T7051 |
T7045 |
punct |
),S4 |
R2819 |
T7052 |
T7016 |
punct |
.,restricted |
R2820 |
T7054 |
T7055 |
nsubj |
We,cultured |
R2821 |
T7056 |
T7055 |
advmod |
next,cultured |
R2822 |
T7057 |
T7058 |
nmod |
E14.5,explants |
R2823 |
T7058 |
T7055 |
dobj |
explants,cultured |
R2824 |
T7059 |
T7058 |
amod |
whole,explants |
R2825 |
T7060 |
T7058 |
compound |
DRG,explants |
R2826 |
T7061 |
T7058 |
prep |
from,explants |
R2827 |
T7062 |
T7063 |
nmod |
TauEWS,PVCre |
R2828 |
T7063 |
T7068 |
nmod |
PVCre,mice |
R2829 |
T7064 |
T7063 |
punct |
-,PVCre |
R2830 |
T7065 |
T7063 |
nmod |
Pea3,PVCre |
R2831 |
T7066 |
T7063 |
punct |
/,PVCre |
R2832 |
T7067 |
T7063 |
punct |
+,PVCre |
R2833 |
T7068 |
T7061 |
pobj |
mice,from |
R2834 |
T7069 |
T7063 |
punct |
/,PVCre |
R2835 |
T7070 |
T7063 |
punct |
+,PVCre |
R2836 |
T7071 |
T7063 |
cc |
and,PVCre |
R2837 |
T7072 |
T7073 |
nmod |
TaumGFP,PVCre |
R2838 |
T7073 |
T7063 |
conj |
PVCre,PVCre |
R2839 |
T7074 |
T7073 |
punct |
/,PVCre |
R2840 |
T7075 |
T7073 |
punct |
+,PVCre |
R2841 |
T7076 |
T7073 |
punct |
/,PVCre |
R2842 |
T7077 |
T7073 |
punct |
+,PVCre |
R2843 |
T7078 |
T7055 |
prep |
for,cultured |
R2844 |
T7079 |
T7080 |
nummod |
48,h |
R2845 |
T7080 |
T7078 |
pobj |
h,for |
R2846 |
T7081 |
T7082 |
advmod |
in,vitro |
R2847 |
T7082 |
T7080 |
advmod |
vitro,h |
R2848 |
T7083 |
T7055 |
prep |
in,cultured |
R2849 |
T7084 |
T7085 |
det |
the,presence |
R2850 |
T7085 |
T7083 |
pobj |
presence,in |
R2851 |
T7086 |
T7085 |
cc |
or,presence |
R2852 |
T7087 |
T7085 |
conj |
absence,presence |
R2853 |
T7088 |
T7085 |
prep |
of,presence |
R2854 |
T7089 |
T7088 |
pobj |
NT,of |
R2855 |
T7090 |
T7089 |
punct |
-,NT |
R2856 |
T7091 |
T7089 |
nummod |
3,NT |
R2857 |
T7092 |
T7093 |
punct |
(,Figure |
R2858 |
T7093 |
T7055 |
parataxis |
Figure,cultured |
R2859 |
T7094 |
T7093 |
nummod |
5,Figure |
R2860 |
T7095 |
T7093 |
punct |
),Figure |
R2861 |
T7096 |
T7055 |
punct |
.,cultured |
R2862 |
T7098 |
T7099 |
nsubj |
We,found |
R2863 |
T7100 |
T7101 |
mark |
that,survived |
R2864 |
T7101 |
T7099 |
ccomp |
survived,found |
R2865 |
T7102 |
T7103 |
compound |
DRG,neurons |
R2866 |
T7103 |
T7101 |
nsubj |
neurons,survived |
R2867 |
T7104 |
T7103 |
prep |
from,neurons |
R2868 |
T7105 |
T7106 |
det |
both,genotypes |
R2869 |
T7106 |
T7104 |
pobj |
genotypes,from |
R2870 |
T7107 |
T7101 |
cc |
and,survived |
R2871 |
T7108 |
T7101 |
conj |
extended,survived |
R2872 |
T7109 |
T7108 |
dobj |
neurites,extended |
R2873 |
T7110 |
T7111 |
advmod |
only,in |
R2874 |
T7111 |
T7101 |
prep |
in,survived |
R2875 |
T7112 |
T7113 |
det |
the,presence |
R2876 |
T7113 |
T7111 |
pobj |
presence,in |
R2877 |
T7114 |
T7113 |
prep |
of,presence |
R2878 |
T7115 |
T7114 |
pobj |
NT,of |
R2879 |
T7116 |
T7115 |
punct |
-,NT |
R2880 |
T7117 |
T7115 |
nummod |
3,NT |
R2881 |
T7118 |
T7101 |
punct |
", ",survived |
R2882 |
T7119 |
T7120 |
mark |
whereas,died |
R2883 |
T7120 |
T7101 |
advcl |
died,survived |
R2884 |
T7121 |
T7120 |
nsubj |
they,died |
R2885 |
T7122 |
T7120 |
prep |
in,died |
R2886 |
T7123 |
T7124 |
det |
the,absence |
R2887 |
T7124 |
T7122 |
pobj |
absence,in |
R2888 |
T7125 |
T7124 |
prep |
of,absence |
R2889 |
T7126 |
T7125 |
pobj |
NT,of |
R2890 |
T7127 |
T7126 |
punct |
-,NT |
R2891 |
T7128 |
T7126 |
nummod |
3,NT |
R2892 |
T7129 |
T7130 |
punct |
(,Figure |
R2893 |
T7130 |
T7101 |
parataxis |
Figure,survived |
R2894 |
T7131 |
T7130 |
nummod |
5,Figure |
R2895 |
T7132 |
T7130 |
punct |
),Figure |
R2896 |
T7133 |
T7099 |
punct |
.,found |
R2897 |
T7135 |
T7136 |
advmod |
Together,suggest |
R2898 |
T7137 |
T7136 |
punct |
", ",suggest |
R2899 |
T7138 |
T7139 |
det |
these,findings |
R2900 |
T7139 |
T7136 |
nsubj |
findings,suggest |
R2901 |
T7140 |
T7141 |
mark |
that,is |
R2902 |
T7141 |
T7136 |
ccomp |
is,suggest |
R2903 |
T7142 |
T7143 |
advmod |
only,signaling |
R2904 |
T7143 |
T7141 |
nsubj |
signaling,is |
R2905 |
T7144 |
T7143 |
amod |
precocious,signaling |
R2906 |
T7145 |
T7144 |
cc |
but,precocious |
R2907 |
T7146 |
T7145 |
neg |
not,but |
R2908 |
T7147 |
T7144 |
conj |
isochronic,precocious |
R2909 |
T7148 |
T7143 |
compound |
ETS,signaling |
R2910 |
T7149 |
T7143 |
prep |
in,signaling |
R2911 |
T7150 |
T7151 |
compound |
DRG,neurons |
R2912 |
T7151 |
T7149 |
pobj |
neurons,in |
R2913 |
T7152 |
T7141 |
acomp |
capable,is |
R2914 |
T7153 |
T7152 |
prep |
of,capable |
R2915 |
T7154 |
T7153 |
pcomp |
uncoupling,of |
R2916 |
T7155 |
T7154 |
dobj |
survival,uncoupling |
R2917 |
T7156 |
T7155 |
cc |
and,survival |
R2918 |
T7157 |
T7158 |
compound |
neurite,outgrowth |
R2919 |
T7158 |
T7155 |
conj |
outgrowth,survival |
R2920 |
T7159 |
T7154 |
prep |
from,uncoupling |
R2921 |
T7160 |
T7161 |
det |
a,requirement |
R2922 |
T7161 |
T7159 |
pobj |
requirement,from |
R2923 |
T7162 |
T7161 |
prep |
for,requirement |
R2924 |
T7163 |
T7164 |
compound |
neurotrophin,signaling |
R2925 |
T7164 |
T7162 |
pobj |
signaling,for |
R2926 |
T7165 |
T7166 |
advmod |
normally,observed |
R2927 |
T7166 |
T7161 |
acl |
observed,requirement |
R2928 |
T7167 |
T7166 |
prep |
in,observed |
R2929 |
T7168 |
T7169 |
amod |
wild,type |
R2930 |
T7169 |
T7171 |
compound |
type,DRG |
R2931 |
T7170 |
T7169 |
punct |
-,type |
R2932 |
T7171 |
T7167 |
pobj |
DRG,in |
R2933 |
T7172 |
T7136 |
punct |
.,suggest |
R2934 |
T7174 |
T7175 |
aux |
To,determine |
R2935 |
T7175 |
T7176 |
advcl |
determine,analyzed |
R2936 |
T7177 |
T7178 |
mark |
whether,is |
R2937 |
T7178 |
T7175 |
ccomp |
is,determine |
R2938 |
T7179 |
T7180 |
amod |
neuronal,survival |
R2939 |
T7180 |
T7178 |
nsubj |
survival,is |
R2940 |
T7181 |
T7180 |
prep |
of,survival |
R2941 |
T7182 |
T7183 |
compound |
DRG,neurons |
R2942 |
T7183 |
T7181 |
pobj |
neurons,of |
R2943 |
T7184 |
T7183 |
prep |
from,neurons |
R2944 |
T7185 |
T7186 |
nmod |
TauEWS,Isl1Cre |
R2945 |
T7186 |
T7191 |
nmod |
Isl1Cre,embryos |
R2946 |
T7187 |
T7186 |
punct |
-,Isl1Cre |
R2947 |
T7188 |
T7186 |
nmod |
Pea3,Isl1Cre |
R2948 |
T7189 |
T7186 |
punct |
/,Isl1Cre |
R2949 |
T7190 |
T7186 |
punct |
+,Isl1Cre |
R2950 |
T7191 |
T7184 |
pobj |
embryos,from |
R2951 |
T7192 |
T7186 |
punct |
/,Isl1Cre |
R2952 |
T7193 |
T7186 |
punct |
+,Isl1Cre |
R2953 |
T7194 |
T7180 |
prep |
in,survival |
R2954 |
T7195 |
T7196 |
det |
the,absence |
R2955 |
T7196 |
T7194 |
pobj |
absence,in |
R2956 |
T7197 |
T7196 |
prep |
of,absence |
R2957 |
T7198 |
T7199 |
amod |
neurotrophic,support |
R2958 |
T7199 |
T7197 |
pobj |
support,of |
R2959 |
T7200 |
T7178 |
acomp |
sufficient,is |
R2960 |
T7201 |
T7202 |
aux |
to,explain |
R2961 |
T7202 |
T7200 |
xcomp |
explain,sufficient |
R2962 |
T7203 |
T7204 |
det |
the,outgrowth |
R2963 |
T7204 |
T7202 |
dobj |
outgrowth,explain |
R2964 |
T7205 |
T7204 |
amod |
observed,outgrowth |
R2965 |
T7206 |
T7204 |
amod |
neuronal,outgrowth |
R2966 |
T7207 |
T7176 |
punct |
", ",analyzed |
R2967 |
T7208 |
T7176 |
nsubj |
we,analyzed |
R2968 |
T7209 |
T7176 |
dobj |
DRG,analyzed |
R2969 |
T7210 |
T7209 |
acl |
isolated,DRG |
R2970 |
T7211 |
T7210 |
prep |
from,isolated |
R2971 |
T7212 |
T7213 |
compound |
mice,mutant |
R2972 |
T7213 |
T7211 |
pobj |
mutant,from |
R2973 |
T7214 |
T7176 |
prep |
in,analyzed |
R2974 |
T7215 |
T7216 |
det |
the,Bax |
R2975 |
T7216 |
T7214 |
pobj |
Bax,in |
R2976 |
T7217 |
T7216 |
amod |
proapoptotic,Bax |
R2977 |
T7218 |
T7216 |
compound |
gene,Bax |
R2978 |
T7219 |
T7220 |
punct |
[,27 |
R2979 |
T7220 |
T7176 |
parataxis |
27,analyzed |
R2980 |
T7221 |
T7220 |
punct |
],27 |
R2981 |
T7222 |
T7176 |
punct |
.,analyzed |
R2982 |
T7224 |
T7225 |
advcl |
Consistent,survived |
R2983 |
T7226 |
T7224 |
prep |
with,Consistent |
R2984 |
T7227 |
T7228 |
amod |
previous,results |
R2985 |
T7228 |
T7226 |
pobj |
results,with |
R2986 |
T7229 |
T7225 |
punct |
", ",survived |
R2987 |
T7230 |
T7231 |
nmod |
Bax,neurons |
R2988 |
T7231 |
T7225 |
nsubj |
neurons,survived |
R2989 |
T7232 |
T7230 |
punct |
−,Bax |
R2990 |
T7233 |
T7230 |
punct |
/,Bax |
R2991 |
T7234 |
T7230 |
punct |
−,Bax |
R2992 |
T7235 |
T7231 |
compound |
DRG,neurons |
R2993 |
T7236 |
T7225 |
prep |
without,survived |
R2994 |
T7237 |
T7238 |
amod |
neurotrophic,support |
R2995 |
T7238 |
T7236 |
pobj |
support,without |
R2996 |
T7239 |
T7240 |
punct |
[,28 |
R2997 |
T7240 |
T7225 |
parataxis |
28,survived |
R2998 |
T7241 |
T7240 |
punct |
],28 |
R2999 |
T7242 |
T7225 |
punct |
.,survived |
R3000 |
T7244 |
T7245 |
prep |
In,was |
R3001 |
T7246 |
T7244 |
pobj |
contrast,In |
R3002 |
T7247 |
T7245 |
punct |
", ",was |
R3003 |
T7248 |
T7249 |
compound |
neurite,outgrowth |
R3004 |
T7249 |
T7245 |
nsubj |
outgrowth,was |
R3005 |
T7250 |
T7249 |
prep |
of,outgrowth |
R3006 |
T7251 |
T7252 |
nmod |
Bax,neurons |
R3007 |
T7252 |
T7250 |
pobj |
neurons,of |
R3008 |
T7253 |
T7251 |
punct |
−,Bax |
R3009 |
T7254 |
T7251 |
punct |
/,Bax |
R3010 |
T7255 |
T7251 |
punct |
−,Bax |
R3011 |
T7256 |
T7252 |
compound |
DRG,neurons |
R3012 |
T7257 |
T7258 |
advmod |
significantly,less |
R3013 |
T7258 |
T7245 |
acomp |
less,was |
R3014 |
T7259 |
T7260 |
punct |
(,see |
R3015 |
T7260 |
T7258 |
parataxis |
see,less |
R3016 |
T7261 |
T7262 |
compound |
Figure,4G |
R3017 |
T7262 |
T7260 |
dobj |
4G,see |
R3018 |
T7263 |
T7260 |
punct |
),see |
R3019 |
T7264 |
T7258 |
prep |
than,less |
R3020 |
T7265 |
T7264 |
pobj |
that,than |
R3021 |
T7266 |
T7265 |
prep |
of,that |
R3022 |
T7267 |
T7268 |
preconj |
either,DRG |
R3023 |
T7268 |
T7266 |
pobj |
DRG,of |
R3024 |
T7269 |
T7268 |
prep |
from,DRG |
R3025 |
T7270 |
T7271 |
nmod |
TauEWS,Isl1Cre |
R3026 |
T7271 |
T7276 |
nmod |
Isl1Cre,embryos |
R3027 |
T7272 |
T7271 |
punct |
-,Isl1Cre |
R3028 |
T7273 |
T7271 |
nmod |
Pea3,Isl1Cre |
R3029 |
T7274 |
T7271 |
punct |
/,Isl1Cre |
R3030 |
T7275 |
T7271 |
punct |
+,Isl1Cre |
R3031 |
T7276 |
T7269 |
pobj |
embryos,from |
R3032 |
T7277 |
T7271 |
punct |
/,Isl1Cre |
R3033 |
T7278 |
T7271 |
punct |
+,Isl1Cre |
R3034 |
T7279 |
T7268 |
acl |
cultured,DRG |
R3035 |
T7280 |
T7279 |
prep |
in,cultured |
R3036 |
T7281 |
T7282 |
det |
the,absence |
R3037 |
T7282 |
T7280 |
pobj |
absence,in |
R3038 |
T7283 |
T7282 |
prep |
of,absence |
R3039 |
T7284 |
T7285 |
amod |
neurotrophic,support |
R3040 |
T7285 |
T7283 |
pobj |
support,of |
R3041 |
T7286 |
T7287 |
punct |
(,see |
R3042 |
T7287 |
T7279 |
parataxis |
see,cultured |
R3043 |
T7288 |
T7289 |
compound |
Figure,4D |
R3044 |
T7289 |
T7287 |
dobj |
4D,see |
R3045 |
T7290 |
T7287 |
punct |
),see |
R3046 |
T7291 |
T7268 |
cc |
or,DRG |
R3047 |
T7292 |
T7293 |
nmod |
Bax,neurons |
R3048 |
T7293 |
T7268 |
conj |
neurons,DRG |
R3049 |
T7294 |
T7292 |
punct |
−,Bax |
R3050 |
T7295 |
T7292 |
punct |
/,Bax |
R3051 |
T7296 |
T7292 |
punct |
−,Bax |
R3052 |
T7297 |
T7293 |
compound |
DRG,neurons |
R3053 |
T7298 |
T7293 |
acl |
cultured,neurons |
R3054 |
T7299 |
T7298 |
prep |
in,cultured |
R3055 |
T7300 |
T7301 |
det |
the,presence |
R3056 |
T7301 |
T7299 |
pobj |
presence,in |
R3057 |
T7302 |
T7301 |
prep |
of,presence |
R3058 |
T7303 |
T7304 |
amod |
neurotrophic,support |
R3059 |
T7304 |
T7302 |
pobj |
support,of |
R3060 |
T7305 |
T7306 |
punct |
(,see |
R3061 |
T7306 |
T7298 |
parataxis |
see,cultured |
R3062 |
T7307 |
T7308 |
compound |
Figure,4H |
R3063 |
T7308 |
T7306 |
dobj |
4H,see |
R3064 |
T7309 |
T7308 |
cc |
and,4H |
R3065 |
T7310 |
T7308 |
conj |
4I,4H |
R3066 |
T7311 |
T7306 |
punct |
),see |
R3067 |
T7312 |
T7245 |
punct |
.,was |
R3068 |
T7314 |
T7315 |
det |
These,findings |
R3069 |
T7315 |
T7316 |
nsubj |
findings,suggest |
R3070 |
T7317 |
T7318 |
mark |
that,promotes |
R3071 |
T7318 |
T7316 |
ccomp |
promotes,suggest |
R3072 |
T7319 |
T7318 |
prep |
in,promotes |
R3073 |
T7320 |
T7319 |
pobj |
addition,in |
R3074 |
T7321 |
T7320 |
prep |
to,addition |
R3075 |
T7322 |
T7321 |
pcomp |
mediating,to |
R3076 |
T7323 |
T7324 |
npadvmod |
neurotrophin,independent |
R3077 |
T7324 |
T7326 |
amod |
independent,survival |
R3078 |
T7325 |
T7324 |
punct |
-,independent |
R3079 |
T7326 |
T7322 |
dobj |
survival,mediating |
R3080 |
T7327 |
T7326 |
amod |
neuronal,survival |
R3081 |
T7328 |
T7318 |
punct |
", ",promotes |
R3082 |
T7329 |
T7318 |
nsubj |
expression,promotes |
R3083 |
T7330 |
T7329 |
prep |
of,expression |
R3084 |
T7331 |
T7332 |
compound |
EWS,Pea3 |
R3085 |
T7332 |
T7330 |
pobj |
Pea3,of |
R3086 |
T7333 |
T7332 |
punct |
-,Pea3 |
R3087 |
T7334 |
T7329 |
prep |
in,expression |
R3088 |
T7335 |
T7336 |
amod |
early,neurons |
R3089 |
T7336 |
T7334 |
pobj |
neurons,in |
R3090 |
T7337 |
T7336 |
amod |
post-mitotic,neurons |
R3091 |
T7338 |
T7318 |
advmod |
also,promotes |
R3092 |
T7339 |
T7340 |
compound |
neurite,outgrowth |
R3093 |
T7340 |
T7318 |
dobj |
outgrowth,promotes |
R3094 |
T7341 |
T7318 |
prep |
in,promotes |
R3095 |
T7342 |
T7343 |
det |
a,manner |
R3096 |
T7343 |
T7341 |
pobj |
manner,in |
R3097 |
T7344 |
T7345 |
npadvmod |
neurotrophin,independent |
R3098 |
T7345 |
T7343 |
amod |
independent,manner |
R3099 |
T7346 |
T7345 |
punct |
-,independent |
R3100 |
T7347 |
T7316 |
punct |
.,suggest |
R3101 |
T7349 |
T7350 |
aux |
To,begin |
R3102 |
T7350 |
T7351 |
advcl |
begin,assayed |
R3103 |
T7352 |
T7353 |
aux |
to,assess |
R3104 |
T7353 |
T7350 |
xcomp |
assess,begin |
R3105 |
T7354 |
T7355 |
prep |
at,become |
R3106 |
T7355 |
T7353 |
ccomp |
become,assess |
R3107 |
T7356 |
T7357 |
det |
which,step |
R3108 |
T7357 |
T7354 |
pcomp |
step,at |
R3109 |
T7358 |
T7357 |
prep |
of,step |
R3110 |
T7359 |
T7360 |
det |
the,cascade |
R3111 |
T7360 |
T7358 |
pobj |
cascade,of |
R3112 |
T7361 |
T7360 |
compound |
neurotrophin,cascade |
R3113 |
T7362 |
T7360 |
compound |
signaling,cascade |
R3114 |
T7363 |
T7364 |
compound |
DRG,neurons |
R3115 |
T7364 |
T7355 |
nsubj |
neurons,become |
R3116 |
T7365 |
T7364 |
prep |
from,neurons |
R3117 |
T7366 |
T7367 |
nmod |
TauEWS,Isl1Cre |
R3118 |
T7367 |
T7372 |
nmod |
Isl1Cre,embryos |
R3119 |
T7368 |
T7367 |
punct |
-,Isl1Cre |
R3120 |
T7369 |
T7367 |
nmod |
Pea3,Isl1Cre |
R3121 |
T7370 |
T7367 |
punct |
/,Isl1Cre |
R3122 |
T7371 |
T7367 |
punct |
+,Isl1Cre |
R3123 |
T7372 |
T7365 |
pobj |
embryos,from |
R3124 |
T7373 |
T7367 |
punct |
/,Isl1Cre |
R3125 |
T7374 |
T7367 |
punct |
+,Isl1Cre |
R3126 |
T7375 |
T7355 |
acomp |
unresponsive,become |
R3127 |
T7376 |
T7375 |
prep |
to,unresponsive |
R3128 |
T7377 |
T7378 |
det |
the,addition |
R3129 |
T7378 |
T7376 |
pobj |
addition,to |
R3130 |
T7379 |
T7378 |
prep |
of,addition |
R3131 |
T7380 |
T7379 |
pobj |
neurotrophins,of |
R3132 |
T7381 |
T7351 |
punct |
", ",assayed |
R3133 |
T7382 |
T7351 |
nsubj |
we,assayed |
R3134 |
T7383 |
T7384 |
det |
the,expression |
R3135 |
T7384 |
T7351 |
dobj |
expression,assayed |
R3136 |
T7385 |
T7384 |
prep |
of,expression |
R3137 |
T7386 |
T7387 |
compound |
neurotrophin,receptors |
R3138 |
T7387 |
T7385 |
pobj |
receptors,of |
R3139 |
T7388 |
T7351 |
prep |
in,assayed |
R3140 |
T7389 |
T7390 |
nmod |
TauEWS,Isl1Cre |
R3141 |
T7390 |
T7395 |
nmod |
Isl1Cre,embryos |
R3142 |
T7391 |
T7390 |
punct |
-,Isl1Cre |
R3143 |
T7392 |
T7390 |
nmod |
Pea3,Isl1Cre |
R3144 |
T7393 |
T7390 |
punct |
/,Isl1Cre |
R3145 |
T7394 |
T7390 |
punct |
+,Isl1Cre |
R3146 |
T7395 |
T7388 |
pobj |
embryos,in |
R3147 |
T7396 |
T7390 |
punct |
/,Isl1Cre |
R3148 |
T7397 |
T7390 |
punct |
+,Isl1Cre |
R3149 |
T7398 |
T7399 |
punct |
(,Figure |
R3150 |
T7399 |
T7351 |
parataxis |
Figure,assayed |
R3151 |
T7400 |
T7399 |
nummod |
6,Figure |
R3152 |
T7401 |
T7399 |
punct |
),Figure |
R3153 |
T7402 |
T7351 |
punct |
.,assayed |
R3154 |
T7404 |
T7405 |
mark |
Whereas,marks |
R3155 |
T7405 |
T7417 |
advcl |
marks,showed |
R3156 |
T7406 |
T7405 |
nsubj |
expression,marks |
R3157 |
T7407 |
T7406 |
prep |
of,expression |
R3158 |
T7408 |
T7409 |
det |
the,receptors |
R3159 |
T7409 |
T7407 |
pobj |
receptors,of |
R3160 |
T7410 |
T7409 |
compound |
neurotrophin,receptors |
R3161 |
T7411 |
T7409 |
appos |
TrkA,receptors |
R3162 |
T7412 |
T7411 |
punct |
", ",TrkA |
R3163 |
T7413 |
T7411 |
conj |
TrkB,TrkA |
R3164 |
T7414 |
T7413 |
punct |
", ",TrkB |
R3165 |
T7415 |
T7413 |
cc |
and,TrkB |
R3166 |
T7416 |
T7413 |
conj |
TrkC,TrkB |
R3167 |
T7418 |
T7405 |
dobj |
afferents,marks |
R3168 |
T7419 |
T7418 |
prep |
of,afferents |
R3169 |
T7420 |
T7421 |
amod |
distinct,modalities |
R3170 |
T7421 |
T7419 |
pobj |
modalities,of |
R3171 |
T7422 |
T7421 |
amod |
sensory,modalities |
R3172 |
T7423 |
T7405 |
prep |
in,marks |
R3173 |
T7424 |
T7423 |
pobj |
DRG,in |
R3174 |
T7425 |
T7424 |
prep |
of,DRG |
R3175 |
T7426 |
T7427 |
amod |
wild,type |
R3176 |
T7427 |
T7429 |
compound |
type,embryos |
R3177 |
T7428 |
T7427 |
punct |
-,type |
R3178 |
T7429 |
T7425 |
pobj |
embryos,of |
R3179 |
T7430 |
T7431 |
punct |
(,6A |
R3180 |
T7431 |
T7405 |
parataxis |
6A,marks |
R3181 |
T7432 |
T7431 |
compound |
Figure,6A |
R3182 |
T7433 |
T7434 |
punct |
–,6C |
R3183 |
T7434 |
T7431 |
prep |
6C,6A |
R3184 |
T7435 |
T7431 |
punct |
),6A |
R3185 |
T7436 |
T7437 |
punct |
[,29 |
R3186 |
T7437 |
T7405 |
parataxis |
29,marks |
R3187 |
T7438 |
T7437 |
dep |
4,29 |
R3188 |
T7439 |
T7437 |
punct |
",",29 |
R3189 |
T7440 |
T7437 |
punct |
],29 |
R3190 |
T7441 |
T7417 |
punct |
", ",showed |
R3191 |
T7442 |
T7443 |
nmod |
TauEWS,Isl1Cre |
R3192 |
T7443 |
T7448 |
nmod |
Isl1Cre,embryos |
R3193 |
T7444 |
T7443 |
punct |
-,Isl1Cre |
R3194 |
T7445 |
T7443 |
nmod |
Pea3,Isl1Cre |
R3195 |
T7446 |
T7443 |
punct |
/,Isl1Cre |
R3196 |
T7447 |
T7443 |
punct |
+,Isl1Cre |
R3197 |
T7448 |
T7417 |
nsubj |
embryos,showed |
R3198 |
T7449 |
T7443 |
punct |
/,Isl1Cre |
R3199 |
T7450 |
T7443 |
punct |
+,Isl1Cre |
R3200 |
T7451 |
T7452 |
amod |
complete,absence |
R3201 |
T7452 |
T7417 |
dobj |
absence,showed |
R3202 |
T7453 |
T7452 |
prep |
of,absence |
R3203 |
T7454 |
T7453 |
pobj |
expression,of |
R3204 |
T7455 |
T7454 |
prep |
of,expression |
R3205 |
T7456 |
T7455 |
pobj |
TrkA,of |
R3206 |
T7457 |
T7456 |
punct |
", ",TrkA |
R3207 |
T7458 |
T7456 |
conj |
TrkB,TrkA |
R3208 |
T7459 |
T7458 |
punct |
", ",TrkB |
R3209 |
T7460 |
T7458 |
cc |
and,TrkB |
R3210 |
T7461 |
T7458 |
conj |
TrkC,TrkB |
R3211 |
T7462 |
T7454 |
prep |
in,expression |
R3212 |
T7463 |
T7464 |
compound |
DRG,neurons |
R3213 |
T7464 |
T7462 |
pobj |
neurons,in |
R3214 |
T7465 |
T7417 |
prep |
at,showed |
R3215 |
T7466 |
T7465 |
pobj |
E16.5,at |
R3216 |
T7467 |
T7468 |
punct |
(,6G |
R3217 |
T7468 |
T7417 |
parataxis |
6G,showed |
R3218 |
T7469 |
T7468 |
compound |
Figure,6G |
R3219 |
T7470 |
T7471 |
punct |
–,6I |
R3220 |
T7471 |
T7468 |
prep |
6I,6G |
R3221 |
T7472 |
T7468 |
punct |
),6G |
R3222 |
T7473 |
T7417 |
punct |
.,showed |
R3223 |
T7475 |
T7476 |
det |
This,absence |
R3224 |
T7476 |
T7477 |
nsubj |
absence,provides |
R3225 |
T7478 |
T7476 |
prep |
of,absence |
R3226 |
T7479 |
T7480 |
compound |
Trk,expression |
R3227 |
T7480 |
T7478 |
pobj |
expression,of |
R3228 |
T7481 |
T7480 |
compound |
receptor,expression |
R3229 |
T7482 |
T7476 |
prep |
in,absence |
R3230 |
T7483 |
T7482 |
pobj |
DRG,in |
R3231 |
T7484 |
T7483 |
prep |
of,DRG |
R3232 |
T7485 |
T7486 |
nmod |
TauEWS,Isl1Cre |
R3233 |
T7486 |
T7491 |
nmod |
Isl1Cre,embryos |
R3234 |
T7487 |
T7486 |
punct |
-,Isl1Cre |
R3235 |
T7488 |
T7486 |
nmod |
Pea3,Isl1Cre |
R3236 |
T7489 |
T7486 |
punct |
/,Isl1Cre |
R3237 |
T7490 |
T7486 |
punct |
+,Isl1Cre |
R3238 |
T7491 |
T7484 |
pobj |
embryos,of |
R3239 |
T7492 |
T7486 |
punct |
/,Isl1Cre |
R3240 |
T7493 |
T7486 |
punct |
+,Isl1Cre |
R3241 |
T7494 |
T7495 |
det |
a,explanation |
R3242 |
T7495 |
T7477 |
dobj |
explanation,provides |
R3243 |
T7496 |
T7495 |
amod |
likely,explanation |
R3244 |
T7497 |
T7495 |
prep |
for,explanation |
R3245 |
T7498 |
T7499 |
det |
the,lack |
R3246 |
T7499 |
T7497 |
pobj |
lack,for |
R3247 |
T7500 |
T7499 |
prep |
of,lack |
R3248 |
T7501 |
T7500 |
pobj |
responsiveness,of |
R3249 |
T7502 |
T7501 |
prep |
of,responsiveness |
R3250 |
T7503 |
T7504 |
det |
these,neurons |
R3251 |
T7504 |
T7502 |
pobj |
neurons,of |
R3252 |
T7505 |
T7501 |
prep |
to,responsiveness |
R3253 |
T7506 |
T7507 |
det |
the,addition |
R3254 |
T7507 |
T7505 |
pobj |
addition,to |
R3255 |
T7508 |
T7507 |
prep |
of,addition |
R3256 |
T7509 |
T7510 |
amod |
neurotrophic,factors |
R3257 |
T7510 |
T7508 |
pobj |
factors,of |
R3258 |
T7511 |
T7477 |
punct |
.,provides |
R3259 |
T7513 |
T7514 |
nsubj |
We,assayed |
R3260 |
T7515 |
T7514 |
advmod |
next,assayed |
R3261 |
T7516 |
T7517 |
mark |
whether,had |
R3262 |
T7517 |
T7514 |
ccomp |
had,assayed |
R3263 |
T7518 |
T7519 |
det |
the,absence |
R3264 |
T7519 |
T7517 |
nsubj |
absence,had |
R3265 |
T7520 |
T7519 |
amod |
complete,absence |
R3266 |
T7521 |
T7519 |
prep |
of,absence |
R3267 |
T7522 |
T7523 |
compound |
Trk,expression |
R3268 |
T7523 |
T7521 |
pobj |
expression,of |
R3269 |
T7524 |
T7523 |
compound |
receptor,expression |
R3270 |
T7525 |
T7519 |
prep |
in,absence |
R3271 |
T7526 |
T7527 |
nmod |
TauEWS,Isl1Cre |
R3272 |
T7527 |
T7532 |
nmod |
Isl1Cre,embryos |
R3273 |
T7528 |
T7527 |
punct |
-,Isl1Cre |
R3274 |
T7529 |
T7527 |
nmod |
Pea3,Isl1Cre |
R3275 |
T7530 |
T7527 |
punct |
/,Isl1Cre |
R3276 |
T7531 |
T7527 |
punct |
+,Isl1Cre |
R3277 |
T7532 |
T7525 |
pobj |
embryos,in |
R3278 |
T7533 |
T7527 |
punct |
/,Isl1Cre |
R3279 |
T7534 |
T7527 |
punct |
+,Isl1Cre |
R3280 |
T7535 |
T7536 |
det |
an,influence |
R3281 |
T7536 |
T7517 |
dobj |
influence,had |
R3282 |
T7537 |
T7536 |
prep |
on,influence |
R3283 |
T7538 |
T7539 |
advmod |
naturally,occurring |
R3284 |
T7539 |
T7540 |
amod |
occurring,death |
R3285 |
T7540 |
T7537 |
pobj |
death,on |
R3286 |
T7541 |
T7540 |
compound |
cell,death |
R3287 |
T7542 |
T7543 |
advmod |
in,vivo |
R3288 |
T7543 |
T7540 |
advmod |
vivo,death |
R3289 |
T7544 |
T7514 |
advcl |
using,assayed |
R3290 |
T7545 |
T7544 |
dobj |
TUNEL,using |
R3291 |
T7546 |
T7544 |
prep |
on,using |
R3292 |
T7547 |
T7548 |
compound |
DRG,sections |
R3293 |
T7548 |
T7546 |
pobj |
sections,on |
R3294 |
T7549 |
T7514 |
punct |
.,assayed |
R3295 |
T7551 |
T7552 |
advmod |
Surprisingly,found |
R3296 |
T7553 |
T7552 |
punct |
", ",found |
R3297 |
T7554 |
T7552 |
nsubj |
we,found |
R3298 |
T7555 |
T7556 |
mark |
that,decreased |
R3299 |
T7556 |
T7552 |
ccomp |
decreased,found |
R3300 |
T7557 |
T7556 |
nsubjpass |
apoptosis,decreased |
R3301 |
T7558 |
T7556 |
auxpass |
was,decreased |
R3302 |
T7559 |
T7556 |
prep |
by,decreased |
R3303 |
T7560 |
T7561 |
advmod |
approximately,50 |
R3304 |
T7561 |
T7562 |
nummod |
50,% |
R3305 |
T7562 |
T7559 |
pobj |
%,by |
R3306 |
T7563 |
T7564 |
punct |
(,average |
R3307 |
T7564 |
T7562 |
parataxis |
average,% |
R3308 |
T7565 |
T7566 |
nsubj |
n,embryos |
R3309 |
T7566 |
T7564 |
ccomp |
embryos,average |
R3310 |
T7567 |
T7566 |
punct |
=,embryos |
R3311 |
T7568 |
T7566 |
nummod |
3,embryos |
R3312 |
T7569 |
T7564 |
punct |
", ",average |
R3313 |
T7570 |
T7564 |
prep |
of,average |
R3314 |
T7571 |
T7572 |
punct |
>,50 |
R3315 |
T7572 |
T7573 |
nummod |
50,sections |
R3316 |
T7573 |
T7570 |
pobj |
sections,of |
R3317 |
T7574 |
T7564 |
punct |
),average |
R3318 |
T7575 |
T7556 |
prep |
in,decreased |
R3319 |
T7576 |
T7575 |
pobj |
DRG,in |
R3320 |
T7577 |
T7576 |
prep |
of,DRG |
R3321 |
T7578 |
T7579 |
nmod |
TauEWS,Isl1Cre |
R3322 |
T7579 |
T7584 |
nmod |
Isl1Cre,embryos |
R3323 |
T7580 |
T7579 |
punct |
-,Isl1Cre |
R3324 |
T7581 |
T7579 |
nmod |
Pea3,Isl1Cre |
R3325 |
T7582 |
T7579 |
punct |
/,Isl1Cre |
R3326 |
T7583 |
T7579 |
punct |
+,Isl1Cre |
R3327 |
T7584 |
T7577 |
pobj |
embryos,of |
R3328 |
T7585 |
T7579 |
punct |
/,Isl1Cre |
R3329 |
T7586 |
T7579 |
punct |
+,Isl1Cre |
R3330 |
T7587 |
T7556 |
prep |
in,decreased |
R3331 |
T7588 |
T7587 |
pobj |
comparison,in |
R3332 |
T7589 |
T7588 |
prep |
to,comparison |
R3333 |
T7590 |
T7591 |
amod |
wild,type |
R3334 |
T7591 |
T7589 |
pobj |
type,to |
R3335 |
T7592 |
T7591 |
punct |
-,type |
R3336 |
T7593 |
T7594 |
punct |
(,6D |
R3337 |
T7594 |
T7556 |
parataxis |
6D,decreased |
R3338 |
T7595 |
T7594 |
compound |
Figure,6D |
R3339 |
T7596 |
T7594 |
punct |
", ",6D |
R3340 |
T7597 |
T7594 |
conj |
6J,6D |
R3341 |
T7598 |
T7597 |
punct |
", ",6J |
R3342 |
T7599 |
T7597 |
cc |
and,6J |
R3343 |
T7600 |
T7597 |
conj |
6M,6J |
R3344 |
T7601 |
T7594 |
punct |
),6D |
R3345 |
T7602 |
T7552 |
punct |
.,found |
R3346 |
T7604 |
T7605 |
amod |
Quantitative,analysis |
R3347 |
T7605 |
T7606 |
nsubj |
analysis,revealed |
R3348 |
T7607 |
T7605 |
prep |
of,analysis |
R3349 |
T7608 |
T7609 |
det |
the,number |
R3350 |
T7609 |
T7607 |
pobj |
number,of |
R3351 |
T7610 |
T7609 |
prep |
of,number |
R3352 |
T7611 |
T7610 |
pobj |
neurons,of |
R3353 |
T7612 |
T7611 |
prep |
in,neurons |
R3354 |
T7613 |
T7614 |
compound |
lumbar,DRG |
R3355 |
T7614 |
T7612 |
pobj |
DRG,in |
R3356 |
T7615 |
T7614 |
prep |
of,DRG |
R3357 |
T7616 |
T7617 |
nmod |
TauEWS,Isl1Cre |
R3358 |
T7617 |
T7622 |
nmod |
Isl1Cre,embryos |
R3359 |
T7618 |
T7617 |
punct |
-,Isl1Cre |
R3360 |
T7619 |
T7617 |
nmod |
Pea3,Isl1Cre |
R3361 |
T7620 |
T7617 |
punct |
/,Isl1Cre |
R3362 |
T7621 |
T7617 |
punct |
+,Isl1Cre |
R3363 |
T7622 |
T7615 |
pobj |
embryos,of |
R3364 |
T7623 |
T7617 |
punct |
/,Isl1Cre |
R3365 |
T7624 |
T7617 |
punct |
+,Isl1Cre |
R3366 |
T7625 |
T7626 |
det |
a,increase |
R3367 |
T7626 |
T7606 |
dobj |
increase,revealed |
R3368 |
T7627 |
T7626 |
amod |
significant,increase |
R3369 |
T7628 |
T7626 |
prep |
to,increase |
R3370 |
T7629 |
T7630 |
advmod |
approximately,170 |
R3371 |
T7630 |
T7631 |
nummod |
170,% |
R3372 |
T7631 |
T7628 |
pobj |
%,to |
R3373 |
T7632 |
T7631 |
prep |
of,% |
R3374 |
T7633 |
T7634 |
amod |
wild,type |
R3375 |
T7634 |
T7636 |
compound |
type,levels |
R3376 |
T7635 |
T7634 |
punct |
-,type |
R3377 |
T7636 |
T7632 |
pobj |
levels,of |
R3378 |
T7637 |
T7638 |
punct |
(,6E |
R3379 |
T7638 |
T7606 |
parataxis |
6E,revealed |
R3380 |
T7639 |
T7638 |
compound |
Figure,6E |
R3381 |
T7640 |
T7638 |
punct |
", ",6E |
R3382 |
T7641 |
T7638 |
conj |
6K,6E |
R3383 |
T7642 |
T7641 |
punct |
", ",6K |
R3384 |
T7643 |
T7641 |
cc |
and,6K |
R3385 |
T7644 |
T7641 |
conj |
6N,6K |
R3386 |
T7645 |
T7638 |
punct |
),6E |
R3387 |
T7646 |
T7606 |
punct |
.,revealed |
R3388 |
T7648 |
T7649 |
advmod |
Moreover,ruled |
R3389 |
T7650 |
T7649 |
punct |
", ",ruled |
R3390 |
T7651 |
T7652 |
compound |
BrdU,experiments |
R3391 |
T7652 |
T7649 |
nsubj |
experiments,ruled |
R3392 |
T7653 |
T7654 |
compound |
pulse,chase |
R3393 |
T7654 |
T7652 |
compound |
chase,experiments |
R3394 |
T7655 |
T7654 |
punct |
-,chase |
R3395 |
T7656 |
T7649 |
prt |
out,ruled |
R3396 |
T7657 |
T7658 |
det |
the,possibility |
R3397 |
T7658 |
T7649 |
dobj |
possibility,ruled |
R3398 |
T7659 |
T7660 |
mark |
that,reenter |
R3399 |
T7660 |
T7658 |
acl |
reenter,possibility |
R3400 |
T7661 |
T7662 |
compound |
DRG,neurons |
R3401 |
T7662 |
T7660 |
nsubj |
neurons,reenter |
R3402 |
T7663 |
T7662 |
prep |
in,neurons |
R3403 |
T7664 |
T7665 |
nmod |
TauEWS,Isl1Cre |
R3404 |
T7665 |
T7670 |
nmod |
Isl1Cre,embryos |
R3405 |
T7666 |
T7665 |
punct |
-,Isl1Cre |
R3406 |
T7667 |
T7665 |
nmod |
Pea3,Isl1Cre |
R3407 |
T7668 |
T7665 |
punct |
/,Isl1Cre |
R3408 |
T7669 |
T7665 |
punct |
+,Isl1Cre |
R3409 |
T7670 |
T7663 |
pobj |
embryos,in |
R3410 |
T7671 |
T7665 |
punct |
/,Isl1Cre |
R3411 |
T7672 |
T7665 |
punct |
+,Isl1Cre |
R3412 |
T7673 |
T7674 |
det |
the,cycle |
R3413 |
T7674 |
T7660 |
dobj |
cycle,reenter |
R3414 |
T7675 |
T7674 |
compound |
cell,cycle |
R3415 |
T7676 |
T7677 |
punct |
(,6F |
R3416 |
T7677 |
T7660 |
parataxis |
6F,reenter |
R3417 |
T7678 |
T7679 |
det |
no,cells |
R3418 |
T7679 |
T7677 |
dep |
cells,6F |
R3419 |
T7680 |
T7681 |
nmod |
BrdU,LacZ |
R3420 |
T7681 |
T7679 |
nmod |
LacZ,cells |
R3421 |
T7682 |
T7681 |
punct |
+,LacZ |
R3422 |
T7683 |
T7681 |
punct |
/,LacZ |
R3423 |
T7684 |
T7681 |
punct |
+,LacZ |
R3424 |
T7685 |
T7677 |
punct |
", ",6F |
R3425 |
T7686 |
T7687 |
nsubj |
n,embryos |
R3426 |
T7687 |
T7677 |
ccomp |
embryos,6F |
R3427 |
T7688 |
T7687 |
punct |
=,embryos |
R3428 |
T7689 |
T7687 |
nummod |
3,embryos |
R3429 |
T7690 |
T7677 |
punct |
", ",6F |
R3430 |
T7691 |
T7677 |
dep |
analysis,6F |
R3431 |
T7692 |
T7691 |
prep |
of,analysis |
R3432 |
T7693 |
T7694 |
punct |
>,50 |
R3433 |
T7694 |
T7695 |
nummod |
50,sections |
R3434 |
T7695 |
T7692 |
pobj |
sections,of |
R3435 |
T7751 |
T7748 |
conj |
support,receptors |
R3436 |
T7696 |
T7695 |
advmod |
each,sections |
R3437 |
T7752 |
T7705 |
punct |
.,suggest |
R3438 |
T7697 |
T7677 |
punct |
;,6F |
R3439 |
T7754 |
T7755 |
nsubj |
We,analyzed |
R3440 |
T7698 |
T7677 |
compound |
Figure,6F |
R3441 |
T7756 |
T7755 |
advmod |
next,analyzed |
R3442 |
T7757 |
T7758 |
mark |
whether,detected |
R3443 |
T7699 |
T7677 |
cc |
and,6F |
R3444 |
T7758 |
T7755 |
ccomp |
detected,analyzed |
R3445 |
T7759 |
T7758 |
nsubjpass |
changes,detected |
R3446 |
T7760 |
T7759 |
prep |
in,changes |
R3447 |
T7700 |
T7677 |
conj |
6L,6F |
R3448 |
T7761 |
T7762 |
det |
the,expression |
R3449 |
T7762 |
T7760 |
pobj |
expression,in |
R3450 |
T7763 |
T7762 |
prep |
of,expression |
R3451 |
T7701 |
T7677 |
punct |
),6F |
R3452 |
T7764 |
T7763 |
pobj |
proteins,of |
R3453 |
T7765 |
T7764 |
acl |
known,proteins |
R3454 |
T7702 |
T7649 |
punct |
.,ruled |
R3455 |
T7766 |
T7767 |
aux |
to,involved |
R3456 |
T7767 |
T7765 |
xcomp |
involved,known |
R3457 |
T7768 |
T7767 |
auxpass |
be,involved |
R3458 |
T7704 |
T7705 |
advmod |
Together,suggest |
R3459 |
T7769 |
T7767 |
prep |
in,involved |
R3460 |
T7770 |
T7771 |
det |
the,regulation |
R3461 |
T7771 |
T7769 |
pobj |
regulation,in |
R3462 |
T7772 |
T7771 |
prep |
of,regulation |
R3463 |
T7773 |
T7774 |
amod |
neuronal,survival |
R3464 |
T7706 |
T7704 |
prep |
with,Together |
R3465 |
T7774 |
T7772 |
pobj |
survival,of |
R3466 |
T7775 |
T7774 |
cc |
or,survival |
R3467 |
T7776 |
T7777 |
compound |
cell,death |
R3468 |
T7707 |
T7708 |
det |
the,experiments |
R3469 |
T7777 |
T7774 |
conj |
death,survival |
R3470 |
T7778 |
T7758 |
aux |
could,detected |
R3471 |
T7779 |
T7758 |
auxpass |
be,detected |
R3472 |
T7708 |
T7706 |
pobj |
experiments,with |
R3473 |
T7780 |
T7758 |
prep |
in,detected |
R3474 |
T7781 |
T7780 |
pobj |
DRG,in |
R3475 |
T7782 |
T7781 |
prep |
of,DRG |
R3476 |
T7709 |
T7710 |
advmod |
in,vitro |
R3477 |
T7783 |
T7784 |
nmod |
TauEWS,Isl1Cre |
R3478 |
T7784 |
T7789 |
nmod |
Isl1Cre,embryos |
R3479 |
T7710 |
T7708 |
advmod |
vitro,experiments |
R3480 |
T7785 |
T7784 |
punct |
-,Isl1Cre |
R3481 |
T7786 |
T7784 |
nmod |
Pea3,Isl1Cre |
R3482 |
T7787 |
T7784 |
punct |
/,Isl1Cre |
R3483 |
T7711 |
T7708 |
compound |
culture,experiments |
R3484 |
T7788 |
T7784 |
punct |
+,Isl1Cre |
R3485 |
T7789 |
T7782 |
pobj |
embryos,of |
R3486 |
T7790 |
T7784 |
punct |
/,Isl1Cre |
R3487 |
T7791 |
T7784 |
punct |
+,Isl1Cre |
R3488 |
T7792 |
T7755 |
punct |
.,analyzed |
R3489 |
T7712 |
T7705 |
punct |
", ",suggest |
R3490 |
T7794 |
T7795 |
nsubj |
We,found |
R3491 |
T7796 |
T7797 |
det |
no,changes |
R3492 |
T7713 |
T7714 |
det |
these,findings |
R3493 |
T7797 |
T7795 |
dobj |
changes,found |
R3494 |
T7798 |
T7797 |
amod |
significant,changes |
R3495 |
T7799 |
T7797 |
amod |
quantitative,changes |
R3496 |
T7714 |
T7705 |
nsubj |
findings,suggest |
R3497 |
T7800 |
T7797 |
prep |
in,changes |
R3498 |
T7801 |
T7802 |
det |
the,level |
R3499 |
T7715 |
T7716 |
mark |
that,remain |
R3500 |
T7716 |
T7705 |
ccomp |
remain,suggest |
R3501 |
T7802 |
T7800 |
pobj |
level,in |
R3502 |
T7803 |
T7802 |
prep |
of,level |
R3503 |
T7717 |
T7718 |
compound |
DRG,neurons |
R3504 |
T7804 |
T7805 |
compound |
Akt,Akt |
R3505 |
T7805 |
T7803 |
pobj |
Akt,of |
R3506 |
T7806 |
T7805 |
punct |
/,Akt |
R3507 |
T7718 |
T7716 |
nsubj |
neurons,remain |
R3508 |
T7807 |
T7805 |
compound |
p,Akt |
R3509 |
T7808 |
T7805 |
punct |
-,Akt |
R3510 |
T7809 |
T7805 |
cc |
or,Akt |
R3511 |
T7719 |
T7718 |
prep |
from,neurons |
R3512 |
T7810 |
T7811 |
compound |
CREB,CREB |
R3513 |
T7811 |
T7805 |
conj |
CREB,Akt |
R3514 |
T7720 |
T7721 |
nmod |
TauEWS,Isl1Cre |
R3515 |
T7812 |
T7811 |
punct |
/,CREB |
R3516 |
T7813 |
T7811 |
compound |
p,CREB |
R3517 |
T7721 |
T7726 |
nmod |
Isl1Cre,embryos |
R3518 |
T7814 |
T7811 |
punct |
-,CREB |
R3519 |
T7815 |
T7802 |
prep |
in,level |
R3520 |
T7816 |
T7815 |
pobj |
DRG,in |
R3521 |
T7722 |
T7721 |
punct |
-,Isl1Cre |
R3522 |
T7817 |
T7818 |
punct |
(,6O |
R3523 |
T7818 |
T7816 |
parataxis |
6O,DRG |
R3524 |
T7723 |
T7721 |
nmod |
Pea3,Isl1Cre |
R3525 |
T7819 |
T7818 |
compound |
Figure,6O |
R3526 |
T7820 |
T7818 |
cc |
and,6O |
R3527 |
T7821 |
T7818 |
conj |
6P,6O |
R3528 |
T7724 |
T7721 |
punct |
/,Isl1Cre |
R3529 |
T7822 |
T7818 |
punct |
),6O |
R3530 |
T7823 |
T7824 |
dep |
both,shown |
R3531 |
T7725 |
T7721 |
punct |
+,Isl1Cre |
R3532 |
T7824 |
T7802 |
relcl |
shown,level |
R3533 |
T7825 |
T7823 |
prep |
of,both |
R3534 |
T7826 |
T7825 |
pobj |
which,of |
R3535 |
T7726 |
T7719 |
pobj |
embryos,from |
R3536 |
T7827 |
T7824 |
aux |
have,shown |
R3537 |
T7828 |
T7824 |
auxpass |
been,shown |
R3538 |
T7829 |
T7830 |
aux |
to,be |
R3539 |
T7830 |
T7824 |
xcomp |
be,shown |
R3540 |
T7831 |
T7832 |
amod |
key,regulators |
R3541 |
T7832 |
T7830 |
attr |
regulators,be |
R3542 |
T7727 |
T7721 |
punct |
/,Isl1Cre |
R3543 |
T7833 |
T7832 |
prep |
of,regulators |
R3544 |
T7834 |
T7835 |
amod |
neuronal,survival |
R3545 |
T7835 |
T7833 |
pobj |
survival,of |
R3546 |
T7728 |
T7721 |
punct |
+,Isl1Cre |
R3547 |
T7836 |
T7837 |
punct |
[,29 |
R3548 |
T7837 |
T7824 |
parataxis |
29,shown |
R3549 |
T7838 |
T7837 |
punct |
],29 |
R3550 |
T7729 |
T7716 |
oprd |
post-mitotic,remain |
R3551 |
T7839 |
T7795 |
punct |
.,found |
R3552 |
T7730 |
T7716 |
cc |
but,remain |
R3553 |
T7841 |
T7842 |
advmod |
Moreover,reduced |
R3554 |
T7843 |
T7842 |
punct |
", ",reduced |
R3555 |
T7731 |
T7716 |
conj |
fail,remain |
R3556 |
T7732 |
T7733 |
aux |
to,become |
R3557 |
T7844 |
T7845 |
det |
the,level |
R3558 |
T7845 |
T7842 |
nsubjpass |
level,reduced |
R3559 |
T7733 |
T7731 |
xcomp |
become,fail |
R3560 |
T7846 |
T7845 |
prep |
of,level |
R3561 |
T7847 |
T7848 |
det |
the,Bax |
R3562 |
T7734 |
T7733 |
acomp |
sensitive,become |
R3563 |
T7848 |
T7846 |
pobj |
Bax,of |
R3564 |
T7849 |
T7848 |
amod |
proapoptotic,Bax |
R3565 |
T7850 |
T7848 |
compound |
Bcl2,Bax |
R3566 |
T7735 |
T7734 |
prep |
to,sensitive |
R3567 |
T7851 |
T7848 |
compound |
family,Bax |
R3568 |
T7852 |
T7848 |
compound |
member,Bax |
R3569 |
T7853 |
T7842 |
auxpass |
was,reduced |
R3570 |
T7736 |
T7737 |
advmod |
naturally,occurring |
R3571 |
T7854 |
T7842 |
neg |
not,reduced |
R3572 |
T7855 |
T7842 |
advmod |
significantly,reduced |
R3573 |
T7737 |
T7738 |
amod |
occurring,death |
R3574 |
T7738 |
T7735 |
pobj |
death,to |
R3575 |
T7739 |
T7738 |
compound |
cell,death |
R3576 |
T7740 |
T7731 |
punct |
", ",fail |
R3577 |
T7856 |
T7857 |
punct |
(,6O |
R3578 |
T7857 |
T7842 |
parataxis |
6O,reduced |
R3579 |
T7741 |
T7731 |
cc |
and,fail |
R3580 |
T7858 |
T7857 |
compound |
Figure,6O |
R3581 |
T7859 |
T7857 |
cc |
and,6O |
R3582 |
T7860 |
T7857 |
conj |
6P,6O |
R3583 |
T7742 |
T7731 |
conj |
survive,fail |
R3584 |
T7861 |
T7857 |
punct |
),6O |
R3585 |
T7862 |
T7842 |
punct |
.,reduced |
R3586 |
T7743 |
T7742 |
prep |
in,survive |
R3587 |
T7864 |
T7865 |
prep |
In,increased |
R3588 |
T7744 |
T7745 |
det |
the,absence |
R3589 |
T7866 |
T7864 |
pobj |
contrast,In |
R3590 |
T7867 |
T7865 |
punct |
", ",increased |
R3591 |
T7868 |
T7869 |
det |
the,level |
R3592 |
T7869 |
T7865 |
nsubjpass |
level,increased |
R3593 |
T7870 |
T7869 |
compound |
expression,level |
R3594 |
T7745 |
T7743 |
pobj |
absence,in |
R3595 |
T7871 |
T7869 |
prep |
of,level |
R3596 |
T7872 |
T7873 |
det |
the,members |
R3597 |
T7873 |
T7871 |
pobj |
members,of |
R3598 |
T7746 |
T7745 |
prep |
of,absence |
R3599 |
T7874 |
T7873 |
amod |
anti-apoptotic,members |
R3600 |
T7875 |
T7873 |
compound |
Bcl2,members |
R3601 |
T7876 |
T7873 |
compound |
family,members |
R3602 |
T7747 |
T7748 |
compound |
Trk,receptors |
R3603 |
T7877 |
T7878 |
compound |
Bcl,xl |
R3604 |
T7878 |
T7873 |
appos |
xl,members |
R3605 |
T7879 |
T7878 |
punct |
-,xl |
R3606 |
T7748 |
T7746 |
pobj |
receptors,of |
R3607 |
T7880 |
T7878 |
cc |
and,xl |
R3608 |
T7881 |
T7878 |
conj |
Bcl2,xl |
R3609 |
T7882 |
T7865 |
auxpass |
was,increased |
R3610 |
T7749 |
T7748 |
cc |
and,receptors |
R3611 |
T7883 |
T7865 |
advmod |
significantly,increased |
R3612 |
T7884 |
T7885 |
advmod |
when,compared |
R3613 |
T7885 |
T7865 |
advcl |
compared,increased |
R3614 |
T7750 |
T7751 |
amod |
neurotrophic,support |
R3615 |
T7886 |
T7885 |
prep |
to,compared |
R3616 |
T7887 |
T7888 |
amod |
wild,type |
R3617 |
T7888 |
T7890 |
compound |
type,levels |
R3618 |
T7889 |
T7888 |
punct |
-,type |
R3619 |
T7890 |
T7886 |
pobj |
levels,to |
R3620 |
T7891 |
T7892 |
punct |
(,6O |
R3621 |
T7892 |
T7890 |
parataxis |
6O,levels |
R3622 |
T7893 |
T7894 |
dep |
Bcl2,% |
R3623 |
T7894 |
T7892 |
dep |
%,6O |
R3624 |
T7895 |
T7894 |
punct |
", ",% |
R3625 |
T7896 |
T7894 |
nummod |
157,% |
R3626 |
T7897 |
T7892 |
punct |
;,6O |
R3627 |
T7898 |
T7899 |
compound |
Bcl,xl |
R3628 |
T7899 |
T7901 |
dep |
xl,% |
R3629 |
T7900 |
T7899 |
punct |
-,xl |
R3630 |
T7901 |
T7892 |
dep |
%,6O |
R3631 |
T7902 |
T7901 |
punct |
", ",% |
R3632 |
T7903 |
T7901 |
nummod |
259,% |
R3633 |
T7904 |
T7892 |
punct |
;,6O |
R3634 |
T7905 |
T7892 |
dep |
average,6O |
R3635 |
T7906 |
T7905 |
prep |
of,average |
R3636 |
T7907 |
T7908 |
nsubj |
n,experiments |
R3637 |
T7908 |
T7906 |
pcomp |
experiments,of |
R3638 |
T7909 |
T7908 |
punct |
=,experiments |
R3639 |
T7910 |
T7908 |
nummod |
3,experiments |
R3640 |
T7911 |
T7908 |
amod |
independent,experiments |
R3641 |
T7912 |
T7892 |
punct |
;,6O |
R3642 |
T7913 |
T7892 |
compound |
Figure,6O |
R3643 |
T7914 |
T7892 |
cc |
and,6O |
R3644 |
T7915 |
T7892 |
conj |
6P,6O |
R3645 |
T7916 |
T7892 |
punct |
),6O |
R3646 |
T7917 |
T7865 |
punct |
", ",increased |
R3647 |
T7918 |
T7865 |
advcl |
providing,increased |
R3648 |
T7919 |
T7920 |
det |
a,explanation |
R3649 |
T7920 |
T7918 |
dobj |
explanation,providing |
R3650 |
T7921 |
T7920 |
amod |
potential,explanation |
R3651 |
T7922 |
T7920 |
amod |
molecular,explanation |
R3652 |
T7923 |
T7920 |
prep |
for,explanation |
R3653 |
T7924 |
T7925 |
det |
the,survival |
R3654 |
T7925 |
T7923 |
pobj |
survival,for |
R3655 |
T7926 |
T7925 |
amod |
enhanced,survival |
R3656 |
T7927 |
T7925 |
amod |
neuronal,survival |
R3657 |
T7928 |
T7925 |
prep |
of,survival |
R3658 |
T7929 |
T7930 |
compound |
DRG,neurons |
R3659 |
T7930 |
T7928 |
pobj |
neurons,of |
R3660 |
T7931 |
T7930 |
prep |
of,neurons |
R3661 |
T7932 |
T7933 |
nmod |
TauEWS,Isl1Cre |
R3662 |
T7933 |
T7938 |
nmod |
Isl1Cre,embryos |
R3663 |
T7934 |
T7933 |
punct |
-,Isl1Cre |
R3664 |
T7935 |
T7933 |
nmod |
Pea3,Isl1Cre |
R3665 |
T7936 |
T7933 |
punct |
/,Isl1Cre |
R3666 |
T7937 |
T7933 |
punct |
+,Isl1Cre |
R3667 |
T7938 |
T7931 |
pobj |
embryos,of |
R3668 |
T7939 |
T7933 |
punct |
/,Isl1Cre |
R3669 |
T7940 |
T7933 |
punct |
+,Isl1Cre |
R3670 |
T7941 |
T7925 |
prep |
in,survival |
R3671 |
T7942 |
T7943 |
det |
the,absence |
R3672 |
T7943 |
T7941 |
pobj |
absence,in |
R3673 |
T7944 |
T7943 |
prep |
of,absence |
R3674 |
T7945 |
T7946 |
compound |
Trk,receptor |
R3675 |
T7946 |
T7947 |
compound |
receptor,expression |
R3676 |
T7947 |
T7944 |
pobj |
expression,of |
R3677 |
T7948 |
T7949 |
punct |
[,30 |
R3678 |
T7949 |
T7918 |
parataxis |
30,providing |
R3679 |
T7950 |
T7949 |
punct |
],30 |
R3680 |
T7951 |
T7865 |
punct |
.,increased |