PMC:1084331 / 1222-51048 JSON TXT 6 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function

Id	Subject	Object	Predicate	Lexical cue
T1510	13-21	JJ	denotes	Neuronal
T1511	22-37	NN	denotes	differentiation
T1512	38-40	VBZ	denotes	is
T1513	41-42	DT	denotes	a
T1515	43-53	JJ	denotes	protracted
T1514	54-61	NN	denotes	process
T1516	62-68	IN	denotes	during
T1518	69-74	WDT	denotes	which
T1519	75-80	RB	denotes	newly
T1520	81-90	VBN	denotes	generated
T1521	91-98	NNS	denotes	neurons
T1517	99-106	VBP	denotes	express
T1522	107-115	JJ	denotes	distinct
T1524	116-124	JJ	denotes	cellular
T1525	125-128	CC	denotes	and
T1526	129-138	JJ	denotes	molecular
T1523	139-147	NNS	denotes	programs
T1527	148-150	IN	denotes	at
T1528	151-158	JJ	denotes	precise
T1529	159-164	NNS	denotes	times
T1530	165-171	IN	denotes	during
T1531	172-177	PRP$	denotes	their
T1532	178-188	NN	denotes	maturation
T1533	188-190	:	denotes	:
T1534	190-194	JJ	denotes	long
T1536	194-195	HYPH	denotes	-
T1535	195-203	NN	denotes	distance
T1538	204-208	NN	denotes	axon
T1537	209-218	NN	denotes	outgrowth
T1539	218-220	,	denotes	,
T1540	220-230	JJ	denotes	subsequent
T1542	231-239	JJ	denotes	terminal
T1541	240-249	NN	denotes	branching
T1543	249-251	,	denotes	,
T1544	251-254	CC	denotes	and
T1545	255-262	RB	denotes	finally
T1546	263-277	NN	denotes	synaptogenesis
T1547	277-278	.	denotes	.
T1548	278-494	sentence	denotes	Many important aspects of neuronal character appear to be acquired through the expression of transcription factors at progenitor cell stages, whereas others depend on expression immediately upon cell cycle exit [1].
T1549	279-283	JJ	denotes	Many
T1551	284-293	JJ	denotes	important
T1550	294-301	NNS	denotes	aspects
T1553	302-304	IN	denotes	of
T1554	305-313	JJ	denotes	neuronal
T1555	314-323	NN	denotes	character
T1552	324-330	VBP	denotes	appear
T1556	331-333	TO	denotes	to
T1558	334-336	VB	denotes	be
T1557	337-345	VBN	denotes	acquired
T1559	346-353	IN	denotes	through
T1560	354-357	DT	denotes	the
T1561	358-368	NN	denotes	expression
T1562	369-371	IN	denotes	of
T1563	372-385	NN	denotes	transcription
T1564	386-393	NNS	denotes	factors
T1565	394-396	IN	denotes	at
T1566	397-407	NN	denotes	progenitor
T1568	408-412	NN	denotes	cell
T1567	413-419	NNS	denotes	stages
T1569	419-421	,	denotes	,
T1570	421-428	IN	denotes	whereas
T1572	429-435	NNS	denotes	others
T1571	436-442	VBP	denotes	depend
T1573	443-445	IN	denotes	on
T1574	446-456	NN	denotes	expression
T1575	457-468	RB	denotes	immediately
T1576	469-473	IN	denotes	upon
T1577	474-478	NN	denotes	cell
T1578	479-484	NN	denotes	cycle
T1579	485-489	NN	denotes	exit
T1580	490-491	-LRB-	denotes	[
T1581	491-492	CD	denotes	1
T1582	492-493	-RRB-	denotes	]
T1583	493-494	.	denotes	.
T1584	494-747	sentence	denotes	But whether the orderly expression and activity of transcriptional programs at much later developmental stages, well after cell cycle exit, is an essential step in the progression of neuronal differentiation and circuit assembly has yet to be resolved.
T1585	495-498	CC	denotes	But
T1587	499-506	IN	denotes	whether
T1589	507-510	DT	denotes	the
T1591	511-518	JJ	denotes	orderly
T1590	519-529	NN	denotes	expression
T1592	530-533	CC	denotes	and
T1593	534-542	NN	denotes	activity
T1594	543-545	IN	denotes	of
T1595	546-561	JJ	denotes	transcriptional
T1596	562-570	NNS	denotes	programs
T1597	571-573	IN	denotes	at
T1598	574-578	RB	denotes	much
T1599	579-584	JJ	denotes	later
T1601	585-598	JJ	denotes	developmental
T1600	599-605	NNS	denotes	stages
T1602	605-607	,	denotes	,
T1603	607-611	RB	denotes	well
T1604	612-617	IN	denotes	after
T1605	618-622	NN	denotes	cell
T1606	623-628	NN	denotes	cycle
T1607	629-633	NN	denotes	exit
T1608	633-635	,	denotes	,
T1588	635-637	VBZ	denotes	is
T1609	638-640	DT	denotes	an
T1611	641-650	JJ	denotes	essential
T1610	651-655	NN	denotes	step
T1612	656-658	IN	denotes	in
T1613	659-662	DT	denotes	the
T1614	663-674	NN	denotes	progression
T1615	675-677	IN	denotes	of
T1616	678-686	JJ	denotes	neuronal
T1617	687-702	NN	denotes	differentiation
T1618	703-706	CC	denotes	and
T1619	707-714	NN	denotes	circuit
T1620	715-723	NN	denotes	assembly
T1586	724-727	VBZ	denotes	has
T1621	728-731	RB	denotes	yet
T1622	732-734	TO	denotes	to
T1624	735-737	VB	denotes	be
T1623	738-746	VBN	denotes	resolved
T1625	746-747	.	denotes	.
T1626	747-1023	sentence	denotes	The differentiation of sensory neurons of dorsal root ganglia (DRG) has been studied extensively with respect to inductive events that specify neuronal fate [2,3], as well as the involvement of late target-derived neurotrophic factors in the control of neuronal survival [4].
T1627	748-751	DT	denotes	The
T1628	752-767	NN	denotes	differentiation
T1630	768-770	IN	denotes	of
T1631	771-778	JJ	denotes	sensory
T1632	779-786	NNS	denotes	neurons
T1633	787-789	IN	denotes	of
T1634	790-796	JJ	denotes	dorsal
T1636	797-801	NN	denotes	root
T1635	802-809	NNS	denotes	ganglia
T1637	810-811	-LRB-	denotes	(
T1638	811-814	NN	denotes	DRG
T1639	814-815	-RRB-	denotes	)
T1640	816-819	VBZ	denotes	has
T1641	820-824	VBN	denotes	been
T1629	825-832	VBN	denotes	studied
T1642	833-844	RB	denotes	extensively
T1643	845-849	IN	denotes	with
T1644	850-857	NN	denotes	respect
T1645	858-860	IN	denotes	to
T1646	861-870	JJ	denotes	inductive
T1647	871-877	NNS	denotes	events
T1648	878-882	WDT	denotes	that
T1649	883-890	VBP	denotes	specify
T1650	891-899	JJ	denotes	neuronal
T1651	900-904	NN	denotes	fate
T1652	905-906	-LRB-	denotes	[
T1654	906-907	CD	denotes	2
T1655	907-908	,	denotes	,
T1653	908-909	CD	denotes	3
T1656	909-910	-RRB-	denotes	]
T1657	910-912	,	denotes	,
T1658	912-914	RB	denotes	as
T1660	915-919	RB	denotes	well
T1659	920-922	IN	denotes	as
T1661	923-926	DT	denotes	the
T1662	927-938	NN	denotes	involvement
T1663	939-941	IN	denotes	of
T1664	942-946	JJ	denotes	late
T1666	947-953	NN	denotes	target
T1668	953-954	HYPH	denotes	-
T1667	954-961	VBN	denotes	derived
T1669	962-974	JJ	denotes	neurotrophic
T1665	975-982	NNS	denotes	factors
T1670	983-985	IN	denotes	in
T1671	986-989	DT	denotes	the
T1672	990-997	NN	denotes	control
T1673	998-1000	IN	denotes	of
T1674	1001-1009	JJ	denotes	neuronal
T1675	1010-1018	NN	denotes	survival
T1676	1019-1020	-LRB-	denotes	[
T1677	1020-1021	CD	denotes	4
T1678	1021-1022	-RRB-	denotes	]
T1679	1022-1023	.	denotes	.
T1680	1023-1170	sentence	denotes	Recent evidence has begun to emerge that target-derived factors are also involved in regulating later aspects of neuronal differentiation [5,6,7].
T1681	1024-1030	JJ	denotes	Recent
T1682	1031-1039	NN	denotes	evidence
T1684	1040-1043	VBZ	denotes	has
T1683	1044-1049	VBN	denotes	begun
T1685	1050-1052	TO	denotes	to
T1686	1053-1059	VB	denotes	emerge
T1687	1060-1064	IN	denotes	that
T1689	1065-1071	NN	denotes	target
T1691	1071-1072	HYPH	denotes	-
T1690	1072-1079	VBN	denotes	derived
T1692	1080-1087	NNS	denotes	factors
T1693	1088-1091	VBP	denotes	are
T1694	1092-1096	RB	denotes	also
T1688	1097-1105	VBN	denotes	involved
T1695	1106-1108	IN	denotes	in
T1696	1109-1119	VBG	denotes	regulating
T1697	1120-1125	JJ	denotes	later
T1698	1126-1133	NNS	denotes	aspects
T1699	1134-1136	IN	denotes	of
T1700	1137-1145	JJ	denotes	neuronal
T1701	1146-1161	NN	denotes	differentiation
T1702	1162-1163	-LRB-	denotes	[
T1704	1163-1164	CD	denotes	5
T1705	1164-1165	,	denotes	,
T1706	1165-1166	CD	denotes	6
T1707	1166-1167	,	denotes	,
T1703	1167-1168	CD	denotes	7
T1708	1168-1169	-RRB-	denotes	]
T1709	1169-1170	.	denotes	.
T1710	1170-1420	sentence	denotes	In particular, genetic experiments have addressed the survival-independent role of neurotrophic factors during development by exploiting strains of mice defective both in neurotrophin signaling and in the function of the proapoptotic gene Bax [8,9].
T1711	1171-1173	IN	denotes	In
T1713	1174-1184	JJ	denotes	particular
T1714	1184-1186	,	denotes	,
T1715	1186-1193	JJ	denotes	genetic
T1716	1194-1205	NNS	denotes	experiments
T1717	1206-1210	VBP	denotes	have
T1712	1211-1220	VBN	denotes	addressed
T1718	1221-1224	DT	denotes	the
T1720	1225-1233	NN	denotes	survival
T1722	1233-1234	HYPH	denotes	-
T1721	1234-1245	JJ	denotes	independent
T1719	1246-1250	NN	denotes	role
T1723	1251-1253	IN	denotes	of
T1724	1254-1266	JJ	denotes	neurotrophic
T1725	1267-1274	NNS	denotes	factors
T1726	1275-1281	IN	denotes	during
T1727	1282-1293	NN	denotes	development
T1728	1294-1296	IN	denotes	by
T1729	1297-1307	VBG	denotes	exploiting
T1730	1308-1315	NNS	denotes	strains
T1731	1316-1318	IN	denotes	of
T1732	1319-1323	NNS	denotes	mice
T1733	1324-1333	JJ	denotes	defective
T1734	1334-1338	CC	denotes	both
T1735	1339-1341	IN	denotes	in
T1736	1342-1354	NN	denotes	neurotrophin
T1737	1355-1364	NN	denotes	signaling
T1738	1365-1368	CC	denotes	and
T1739	1369-1371	IN	denotes	in
T1740	1372-1375	DT	denotes	the
T1741	1376-1384	NN	denotes	function
T1742	1385-1387	IN	denotes	of
T1743	1388-1391	DT	denotes	the
T1745	1392-1404	JJ	denotes	proapoptotic
T1746	1405-1409	NN	denotes	gene
T1744	1410-1413	NN	denotes	Bax
T1747	1414-1415	-LRB-	denotes	[
T1749	1415-1416	CD	denotes	8
T1750	1416-1417	,	denotes	,
T1748	1417-1418	CD	denotes	9
T1751	1418-1419	-RRB-	denotes	]
T1752	1419-1420	.	denotes	.
T1753	1420-1609	sentence	denotes	These studies, for example, have revealed that neurotrophin signaling controls the acquisition of peptidergic traits in nociceptive DRG neurons and the control of target innervation [8,9].
T1754	1421-1426	DT	denotes	These
T1755	1427-1434	NNS	denotes	studies
T1757	1434-1436	,	denotes	,
T1758	1436-1439	IN	denotes	for
T1759	1440-1447	NN	denotes	example
T1760	1447-1449	,	denotes	,
T1761	1449-1453	VBP	denotes	have
T1756	1454-1462	VBN	denotes	revealed
T1762	1463-1467	IN	denotes	that
T1764	1468-1480	NN	denotes	neurotrophin
T1765	1481-1490	NN	denotes	signaling
T1763	1491-1499	VBZ	denotes	controls
T1766	1500-1503	DT	denotes	the
T1767	1504-1515	NN	denotes	acquisition
T1768	1516-1518	IN	denotes	of
T1769	1519-1530	JJ	denotes	peptidergic
T1770	1531-1537	NNS	denotes	traits
T1771	1538-1540	IN	denotes	in
T1772	1541-1552	JJ	denotes	nociceptive
T1774	1553-1556	NN	denotes	DRG
T1773	1557-1564	NNS	denotes	neurons
T1775	1565-1568	CC	denotes	and
T1776	1569-1572	DT	denotes	the
T1777	1573-1580	NN	denotes	control
T1778	1581-1583	IN	denotes	of
T1779	1584-1590	NN	denotes	target
T1780	1591-1602	NN	denotes	innervation
T1781	1603-1604	-LRB-	denotes	[
T1783	1604-1605	CD	denotes	8
T1784	1605-1606	,	denotes	,
T1782	1606-1607	CD	denotes	9
T1785	1607-1608	-RRB-	denotes	]
T1786	1608-1609	.	denotes	.
T1787	1609-1742	sentence	denotes	The onset of some transcriptional programs in neurons, however, has also been shown to occur long after neurons exit the cell cycle.
T1788	1610-1613	DT	denotes	The
T1789	1614-1619	NN	denotes	onset
T1791	1620-1622	IN	denotes	of
T1792	1623-1627	DT	denotes	some
T1794	1628-1643	JJ	denotes	transcriptional
T1793	1644-1652	NNS	denotes	programs
T1795	1653-1655	IN	denotes	in
T1796	1656-1663	NNS	denotes	neurons
T1797	1663-1665	,	denotes	,
T1798	1665-1672	RB	denotes	however
T1799	1672-1674	,	denotes	,
T1800	1674-1677	VBZ	denotes	has
T1801	1678-1682	RB	denotes	also
T1802	1683-1687	VBN	denotes	been
T1790	1688-1693	VBN	denotes	shown
T1803	1694-1696	TO	denotes	to
T1804	1697-1702	VB	denotes	occur
T1805	1703-1707	RB	denotes	long
T1807	1708-1713	IN	denotes	after
T1808	1714-1721	NNS	denotes	neurons
T1806	1722-1726	VBP	denotes	exit
T1809	1727-1730	DT	denotes	the
T1811	1731-1735	NN	denotes	cell
T1810	1736-1741	NN	denotes	cycle
T1812	1741-1742	.	denotes	.
T1813	1742-1912	sentence	denotes	An emerging principle from work in Drosophila and vertebrates is that target-derived factors play a crucial role in the induction of these transcriptional programs [10].
T1814	1743-1745	DT	denotes	An
T1816	1746-1754	VBG	denotes	emerging
T1815	1755-1764	NN	denotes	principle
T1818	1765-1769	IN	denotes	from
T1819	1770-1774	NN	denotes	work
T1820	1775-1777	IN	denotes	in
T1821	1778-1788	NNP	denotes	Drosophila
T1822	1789-1792	CC	denotes	and
T1823	1793-1804	NNS	denotes	vertebrates
T1817	1805-1807	VBZ	denotes	is
T1824	1808-1812	IN	denotes	that
T1826	1813-1819	NN	denotes	target
T1828	1819-1820	HYPH	denotes	-
T1827	1820-1827	VBN	denotes	derived
T1829	1828-1835	NNS	denotes	factors
T1825	1836-1840	VBP	denotes	play
T1830	1841-1842	DT	denotes	a
T1832	1843-1850	JJ	denotes	crucial
T1831	1851-1855	NN	denotes	role
T1833	1856-1858	IN	denotes	in
T1834	1859-1862	DT	denotes	the
T1835	1863-1872	NN	denotes	induction
T1836	1873-1875	IN	denotes	of
T1837	1876-1881	DT	denotes	these
T1839	1882-1897	JJ	denotes	transcriptional
T1838	1898-1906	NNS	denotes	programs
T1840	1907-1908	-LRB-	denotes	[
T1841	1908-1910	CD	denotes	10
T1842	1910-1911	-RRB-	denotes	]
T1843	1911-1912	.	denotes	.
T1844	1912-2093	sentence	denotes	In Drosophila, retrograde BMP signals from the target region control the terminal differentiation of a subpopulation of peptidergic neurons expressing Apterous and Squeeze [11,12].
T1845	1913-1915	IN	denotes	In
T1847	1916-1926	NNP	denotes	Drosophila
T1848	1926-1928	,	denotes	,
T1849	1928-1938	JJ	denotes	retrograde
T1851	1939-1942	NN	denotes	BMP
T1850	1943-1950	NNS	denotes	signals
T1852	1951-1955	IN	denotes	from
T1853	1956-1959	DT	denotes	the
T1855	1960-1966	NN	denotes	target
T1854	1967-1973	NN	denotes	region
T1846	1974-1981	VBP	denotes	control
T1856	1982-1985	DT	denotes	the
T1858	1986-1994	JJ	denotes	terminal
T1857	1995-2010	NN	denotes	differentiation
T1859	2011-2013	IN	denotes	of
T1860	2014-2015	DT	denotes	a
T1861	2016-2029	NN	denotes	subpopulation
T1862	2030-2032	IN	denotes	of
T1863	2033-2044	JJ	denotes	peptidergic
T1864	2045-2052	NNS	denotes	neurons
T1865	2053-2063	VBG	denotes	expressing
T1866	2064-2072	NN	denotes	Apterous
T1867	2073-2076	CC	denotes	and
T1868	2077-2084	NN	denotes	Squeeze
T1869	2085-2086	-LRB-	denotes	[
T1871	2086-2088	CD	denotes	11
T1872	2088-2089	,	denotes	,
T1870	2089-2091	CD	denotes	12
T1873	2091-2092	-RRB-	denotes	]
T1874	2092-2093	.	denotes	.
T1875	2093-2359	sentence	denotes	In vertebrates, peripheral neurotrophic signals have been shown to direct the onset of expression of the ETS transcription factors Er81 and Pea3 in DRG sensory neurons and motor neuron pools several days after these neurons have become post-mitotic [9,13,14,15,16].
T1876	2094-2096	IN	denotes	In
T1878	2097-2108	NNS	denotes	vertebrates
T1879	2108-2110	,	denotes	,
T1880	2110-2120	JJ	denotes	peripheral
T1882	2121-2133	JJ	denotes	neurotrophic
T1881	2134-2141	NNS	denotes	signals
T1883	2142-2146	VBP	denotes	have
T1884	2147-2151	VBN	denotes	been
T1877	2152-2157	VBN	denotes	shown
T1885	2158-2160	TO	denotes	to
T1886	2161-2167	VB	denotes	direct
T1887	2168-2171	DT	denotes	the
T1888	2172-2177	NN	denotes	onset
T1889	2178-2180	IN	denotes	of
T1890	2181-2191	NN	denotes	expression
T1891	2192-2194	IN	denotes	of
T1892	2195-2198	DT	denotes	the
T1894	2199-2202	NN	denotes	ETS
T1895	2203-2216	NN	denotes	transcription
T1893	2217-2224	NNS	denotes	factors
T1896	2225-2229	NN	denotes	Er81
T1897	2230-2233	CC	denotes	and
T1898	2234-2238	NN	denotes	Pea3
T1899	2239-2241	IN	denotes	in
T1900	2242-2245	NN	denotes	DRG
T1902	2246-2253	JJ	denotes	sensory
T1901	2254-2261	NNS	denotes	neurons
T1903	2262-2265	CC	denotes	and
T1904	2266-2271	NN	denotes	motor
T1906	2272-2278	NN	denotes	neuron
T1905	2279-2284	NNS	denotes	pools
T1907	2285-2292	JJ	denotes	several
T1908	2293-2297	NNS	denotes	days
T1910	2298-2303	IN	denotes	after
T1911	2304-2309	DT	denotes	these
T1912	2310-2317	NNS	denotes	neurons
T1913	2318-2322	VBP	denotes	have
T1909	2323-2329	VBN	denotes	become
T1914	2330-2342	JJ	denotes	post-mitotic
T1915	2343-2344	-LRB-	denotes	[
T1917	2344-2345	CD	denotes	9
T1918	2345-2346	,	denotes	,
T1919	2346-2348	CD	denotes	13
T1920	2348-2349	,	denotes	,
T1921	2349-2351	CD	denotes	14
T1922	2351-2352	,	denotes	,
T1923	2352-2354	CD	denotes	15
T1924	2354-2355	,	denotes	,
T1916	2355-2357	CD	denotes	16
T1925	2357-2358	-RRB-	denotes	]
T1926	2358-2359	.	denotes	.
T1927	2359-2495	sentence	denotes	Moreover, the induction of Er81 expression in proprioceptive afferents is known to be mediated by peripheral neurotrophin 3 (NT-3) [9].
T1928	2360-2368	RB	denotes	Moreover
T1930	2368-2370	,	denotes	,
T1931	2370-2373	DT	denotes	the
T1932	2374-2383	NN	denotes	induction
T1933	2384-2386	IN	denotes	of
T1934	2387-2391	NN	denotes	Er81
T1935	2392-2402	NN	denotes	expression
T1936	2403-2405	IN	denotes	in
T1937	2406-2420	JJ	denotes	proprioceptive
T1938	2421-2430	NNS	denotes	afferents
T1939	2431-2433	VBZ	denotes	is
T1929	2434-2439	VBN	denotes	known
T1940	2440-2442	TO	denotes	to
T1942	2443-2445	VB	denotes	be
T1941	2446-2454	VBN	denotes	mediated
T1943	2455-2457	IN	denotes	by
T1944	2458-2468	JJ	denotes	peripheral
T1945	2469-2481	NN	denotes	neurotrophin
T1946	2482-2483	CD	denotes	3
T1947	2484-2485	-LRB-	denotes	(
T1948	2485-2487	NN	denotes	NT
T1949	2487-2488	HYPH	denotes	-
T1950	2488-2489	CD	denotes	3
T1951	2489-2490	-RRB-	denotes	)
T1952	2491-2492	-LRB-	denotes	[
T1953	2492-2493	CD	denotes	9
T1954	2493-2494	-RRB-	denotes	]
T1955	2494-2495	.	denotes	.
T1956	2495-2722	sentence	denotes	These two ETS proteins control late aspects of spinal monosynaptic circuit assembly, with Er81 directing proprioceptive sensory neuron differentiation and Pea3 directing motor neuron pool differentiation, respectively [14,15].
T1957	2496-2501	DT	denotes	These
T1959	2502-2505	CD	denotes	two
T1960	2506-2509	NN	denotes	ETS
T1958	2510-2518	NN	denotes	proteins
T1961	2519-2526	VBP	denotes	control
T1962	2527-2531	JJ	denotes	late
T1963	2532-2539	NNS	denotes	aspects
T1964	2540-2542	IN	denotes	of
T1965	2543-2549	JJ	denotes	spinal
T1967	2550-2562	JJ	denotes	monosynaptic
T1966	2563-2570	NN	denotes	circuit
T1968	2571-2579	NN	denotes	assembly
T1969	2579-2581	,	denotes	,
T1970	2581-2585	IN	denotes	with
T1971	2586-2590	NN	denotes	Er81
T1972	2591-2600	VBG	denotes	directing
T1973	2601-2615	JJ	denotes	proprioceptive
T1975	2616-2623	JJ	denotes	sensory
T1974	2624-2630	NN	denotes	neuron
T1976	2631-2646	NN	denotes	differentiation
T1977	2647-2650	CC	denotes	and
T1978	2651-2655	NN	denotes	Pea3
T1979	2656-2665	VBG	denotes	directing
T1980	2666-2671	NN	denotes	motor
T1982	2672-2678	NN	denotes	neuron
T1981	2679-2683	NN	denotes	pool
T1983	2684-2699	NN	denotes	differentiation
T1984	2699-2701	,	denotes	,
T1985	2701-2713	RB	denotes	respectively
T1986	2714-2715	-LRB-	denotes	[
T1988	2715-2717	CD	denotes	14
T1989	2717-2718	,	denotes	,
T1987	2718-2720	CD	denotes	15
T1990	2720-2721	-RRB-	denotes	]
T1991	2721-2722	.	denotes	.
T1992	2722-2993	sentence	denotes	In particular, in the absence of Er81, achieved by mutation in the gene or by deprivation of peripheral neurotrophin signaling, group Ia proprioceptive afferents fail to invade the ventral spinal cord and to make effective synaptic connections with motor neurons [9,14].
T1993	2723-2725	IN	denotes	In
T1995	2726-2736	JJ	denotes	particular
T1996	2736-2738	,	denotes	,
T1997	2738-2740	IN	denotes	in
T1998	2741-2744	DT	denotes	the
T1999	2745-2752	NN	denotes	absence
T2000	2753-2755	IN	denotes	of
T2001	2756-2760	NN	denotes	Er81
T2002	2760-2762	,	denotes	,
T2003	2762-2770	VBN	denotes	achieved
T2004	2771-2773	IN	denotes	by
T2005	2774-2782	NN	denotes	mutation
T2006	2783-2785	IN	denotes	in
T2007	2786-2789	DT	denotes	the
T2008	2790-2794	NN	denotes	gene
T2009	2795-2797	CC	denotes	or
T2010	2798-2800	IN	denotes	by
T2011	2801-2812	NN	denotes	deprivation
T2012	2813-2815	IN	denotes	of
T2013	2816-2826	JJ	denotes	peripheral
T2015	2827-2839	NN	denotes	neurotrophin
T2014	2840-2849	NN	denotes	signaling
T2016	2849-2851	,	denotes	,
T2017	2851-2856	NN	denotes	group
T2018	2857-2859	NN	denotes	Ia
T2020	2860-2874	JJ	denotes	proprioceptive
T2019	2875-2884	NNS	denotes	afferents
T1994	2885-2889	VBP	denotes	fail
T2021	2890-2892	TO	denotes	to
T2022	2893-2899	VB	denotes	invade
T2023	2900-2903	DT	denotes	the
T2025	2904-2911	JJ	denotes	ventral
T2026	2912-2918	JJ	denotes	spinal
T2024	2919-2923	NN	denotes	cord
T2027	2924-2927	CC	denotes	and
T2028	2928-2930	TO	denotes	to
T2029	2931-2935	VB	denotes	make
T2030	2936-2945	JJ	denotes	effective
T2032	2946-2954	JJ	denotes	synaptic
T2031	2955-2966	NNS	denotes	connections
T2033	2967-2971	IN	denotes	with
T2034	2972-2977	NN	denotes	motor
T2035	2978-2985	NNS	denotes	neurons
T2036	2986-2987	-LRB-	denotes	[
T2038	2987-2988	CD	denotes	9
T2039	2988-2989	,	denotes	,
T2037	2989-2991	CD	denotes	14
T2040	2991-2992	-RRB-	denotes	]
T2041	2992-2993	.	denotes	.
T2042	2993-3202	sentence	denotes	The involvement of target-derived signals in induction of ETS transcription factor expression raises the question of the necessity for the observed delay in the onset of ETS signaling for neuronal maturation.
T2043	2994-2997	DT	denotes	The
T2044	2998-3009	NN	denotes	involvement
T2046	3010-3012	IN	denotes	of
T2047	3013-3019	NN	denotes	target
T2049	3019-3020	HYPH	denotes	-
T2048	3020-3027	VBN	denotes	derived
T2050	3028-3035	NNS	denotes	signals
T2051	3036-3038	IN	denotes	in
T2052	3039-3048	NN	denotes	induction
T2053	3049-3051	IN	denotes	of
T2054	3052-3055	NN	denotes	ETS
T2056	3056-3069	NN	denotes	transcription
T2057	3070-3076	NN	denotes	factor
T2055	3077-3087	NN	denotes	expression
T2045	3088-3094	VBZ	denotes	raises
T2058	3095-3098	DT	denotes	the
T2059	3099-3107	NN	denotes	question
T2060	3108-3110	IN	denotes	of
T2061	3111-3114	DT	denotes	the
T2062	3115-3124	NN	denotes	necessity
T2063	3125-3128	IN	denotes	for
T2064	3129-3132	DT	denotes	the
T2066	3133-3141	VBN	denotes	observed
T2065	3142-3147	NN	denotes	delay
T2067	3148-3150	IN	denotes	in
T2068	3151-3154	DT	denotes	the
T2069	3155-3160	NN	denotes	onset
T2070	3161-3163	IN	denotes	of
T2071	3164-3167	NN	denotes	ETS
T2072	3168-3177	NN	denotes	signaling
T2073	3178-3181	IN	denotes	for
T2074	3182-3190	JJ	denotes	neuronal
T2075	3191-3201	NN	denotes	maturation
T2076	3201-3202	.	denotes	.
T2077	3202-3337	sentence	denotes	Would precocious expression of ETS proteins in post-mitotic neurons also direct the appropriate sensory neuron developmental programs?
T2078	3203-3208	MD	denotes	Would
T2080	3209-3219	JJ	denotes	precocious
T2081	3220-3230	NN	denotes	expression
T2082	3231-3233	IN	denotes	of
T2083	3234-3237	NN	denotes	ETS
T2084	3238-3246	NN	denotes	proteins
T2085	3247-3249	IN	denotes	in
T2086	3250-3262	JJ	denotes	post-mitotic
T2087	3263-3270	NNS	denotes	neurons
T2088	3271-3275	RB	denotes	also
T2079	3276-3282	VB	denotes	direct
T2089	3283-3286	DT	denotes	the
T2091	3287-3298	JJ	denotes	appropriate
T2092	3299-3306	JJ	denotes	sensory
T2093	3307-3313	NN	denotes	neuron
T2094	3314-3327	JJ	denotes	developmental
T2090	3328-3336	NNS	denotes	programs
T2095	3336-3337	.	denotes	?
T2096	3337-3550	sentence	denotes	In this study, we have used mouse genetics to test this general idea, by investigating whether the precise timing of onset of ETS transcription factor signaling is essential for normal sensory neuron development.
T2097	3338-3340	IN	denotes	In
T2099	3341-3345	DT	denotes	this
T2100	3346-3351	NN	denotes	study
T2101	3351-3353	,	denotes	,
T2102	3353-3355	PRP	denotes	we
T2103	3356-3360	VBP	denotes	have
T2098	3361-3365	VBN	denotes	used
T2104	3366-3371	NN	denotes	mouse
T2105	3372-3380	NN	denotes	genetics
T2106	3381-3383	TO	denotes	to
T2107	3384-3388	VB	denotes	test
T2108	3389-3393	DT	denotes	this
T2110	3394-3401	JJ	denotes	general
T2109	3402-3406	NN	denotes	idea
T2111	3406-3408	,	denotes	,
T2112	3408-3410	IN	denotes	by
T2113	3411-3424	VBG	denotes	investigating
T2114	3425-3432	IN	denotes	whether
T2116	3433-3436	DT	denotes	the
T2118	3437-3444	JJ	denotes	precise
T2117	3445-3451	NN	denotes	timing
T2119	3452-3454	IN	denotes	of
T2120	3455-3460	NN	denotes	onset
T2121	3461-3463	IN	denotes	of
T2122	3464-3467	NN	denotes	ETS
T2124	3468-3481	NN	denotes	transcription
T2125	3482-3488	NN	denotes	factor
T2123	3489-3498	NN	denotes	signaling
T2115	3499-3501	VBZ	denotes	is
T2126	3502-3511	JJ	denotes	essential
T2127	3512-3515	IN	denotes	for
T2128	3516-3522	JJ	denotes	normal
T2130	3523-3530	JJ	denotes	sensory
T2131	3531-3537	NN	denotes	neuron
T2129	3538-3549	NN	denotes	development
T2132	3549-3550	.	denotes	.
T2133	3550-3675	sentence	denotes	We have assessed the biological effects of inducing ETS signaling either at the correct developmental time, or precociously.
T2134	3551-3553	PRP	denotes	We
T2136	3554-3558	VBP	denotes	have
T2135	3559-3567	VBN	denotes	assessed
T2137	3568-3571	DT	denotes	the
T2139	3572-3582	JJ	denotes	biological
T2138	3583-3590	NNS	denotes	effects
T2140	3591-3593	IN	denotes	of
T2141	3594-3602	VBG	denotes	inducing
T2142	3603-3606	NN	denotes	ETS
T2143	3607-3616	NN	denotes	signaling
T2144	3617-3623	CC	denotes	either
T2145	3624-3626	IN	denotes	at
T2146	3627-3630	DT	denotes	the
T2148	3631-3638	JJ	denotes	correct
T2149	3639-3652	JJ	denotes	developmental
T2147	3653-3657	NN	denotes	time
T2150	3657-3659	,	denotes	,
T2151	3659-3661	CC	denotes	or
T2152	3662-3674	RB	denotes	precociously
T2153	3674-3675	.	denotes	.
T2154	3675-3853	sentence	denotes	We find that within proprioceptive sensory neurons, the late onset of ETS signaling is essential for the establishment of normal sensory afferent projections in the spinal cord.
T2155	3676-3678	PRP	denotes	We
T2156	3679-3683	VBP	denotes	find
T2157	3684-3688	IN	denotes	that
T2159	3689-3695	IN	denotes	within
T2160	3696-3710	JJ	denotes	proprioceptive
T2162	3711-3718	JJ	denotes	sensory
T2161	3719-3726	NNS	denotes	neurons
T2163	3726-3728	,	denotes	,
T2164	3728-3731	DT	denotes	the
T2166	3732-3736	JJ	denotes	late
T2165	3737-3742	NN	denotes	onset
T2167	3743-3745	IN	denotes	of
T2168	3746-3749	NN	denotes	ETS
T2169	3750-3759	NN	denotes	signaling
T2158	3760-3762	VBZ	denotes	is
T2170	3763-3772	JJ	denotes	essential
T2171	3773-3776	IN	denotes	for
T2172	3777-3780	DT	denotes	the
T2173	3781-3794	NN	denotes	establishment
T2174	3795-3797	IN	denotes	of
T2175	3798-3804	JJ	denotes	normal
T2177	3805-3812	JJ	denotes	sensory
T2178	3813-3821	JJ	denotes	afferent
T2176	3822-3833	NNS	denotes	projections
T2179	3834-3836	IN	denotes	in
T2180	3837-3840	DT	denotes	the
T2182	3841-3847	JJ	denotes	spinal
T2181	3848-3852	NN	denotes	cord
T2183	3852-3853	.	denotes	.
T2184	3853-4072	sentence	denotes	Precocious initiation of ETS signaling in post-mitotic DRG neurons leads to abnormal DRG neuron differentiation characterized by neurotrophin-independent neurite outgrowth and inappropriate profiles of gene expression.
T2185	3854-3864	JJ	denotes	Precocious
T2186	3865-3875	NN	denotes	initiation
T2188	3876-3878	IN	denotes	of
T2189	3879-3882	NN	denotes	ETS
T2190	3883-3892	NN	denotes	signaling
T2191	3893-3895	IN	denotes	in
T2192	3896-3908	JJ	denotes	post-mitotic
T2194	3909-3912	NN	denotes	DRG
T2193	3913-3920	NNS	denotes	neurons
T2187	3921-3926	VBZ	denotes	leads
T2195	3927-3929	IN	denotes	to
T2196	3930-3938	JJ	denotes	abnormal
T2198	3939-3942	NN	denotes	DRG
T2199	3943-3949	NN	denotes	neuron
T2197	3950-3965	NN	denotes	differentiation
T2200	3966-3979	VBN	denotes	characterized
T2201	3980-3982	IN	denotes	by
T2202	3983-3995	NN	denotes	neurotrophin
T2204	3995-3996	HYPH	denotes	-
T2203	3996-4007	JJ	denotes	independent
T2206	4008-4015	NN	denotes	neurite
T2205	4016-4025	NN	denotes	outgrowth
T2207	4026-4029	CC	denotes	and
T2208	4030-4043	JJ	denotes	inappropriate
T2209	4044-4052	NNS	denotes	profiles
T2210	4053-4055	IN	denotes	of
T2211	4056-4060	NN	denotes	gene
T2212	4061-4071	NN	denotes	expression
T2213	4071-4072	.	denotes	.
T2214	4072-4342	sentence	denotes	Our findings reveal that target-triggered inductive signals provide an effective means of ensuring the late onset of expression of transcription factors, and thus an orderly temporal transcriptional sequence that is crucial for neuronal maturation and circuit assembly.
T2215	4073-4076	PRP$	denotes	Our
T2216	4077-4085	NNS	denotes	findings
T2217	4086-4092	VBP	denotes	reveal
T2218	4093-4097	IN	denotes	that
T2220	4098-4104	NN	denotes	target
T2222	4104-4105	HYPH	denotes	-
T2221	4105-4114	VBN	denotes	triggered
T2224	4115-4124	JJ	denotes	inductive
T2223	4125-4132	NNS	denotes	signals
T2219	4133-4140	VBP	denotes	provide
T2225	4141-4143	DT	denotes	an
T2227	4144-4153	JJ	denotes	effective
T2226	4154-4159	NNS	denotes	means
T2228	4160-4162	IN	denotes	of
T2229	4163-4171	VBG	denotes	ensuring
T2230	4172-4175	DT	denotes	the
T2232	4176-4180	JJ	denotes	late
T2231	4181-4186	NN	denotes	onset
T2233	4187-4189	IN	denotes	of
T2234	4190-4200	NN	denotes	expression
T2235	4201-4203	IN	denotes	of
T2236	4204-4217	NN	denotes	transcription
T2237	4218-4225	NNS	denotes	factors
T2238	4225-4227	,	denotes	,
T2239	4227-4230	CC	denotes	and
T2240	4231-4235	RB	denotes	thus
T2242	4236-4238	DT	denotes	an
T2243	4239-4246	JJ	denotes	orderly
T2244	4247-4255	JJ	denotes	temporal
T2245	4256-4271	JJ	denotes	transcriptional
T2241	4272-4280	NN	denotes	sequence
T2246	4281-4285	WDT	denotes	that
T2247	4286-4288	VBZ	denotes	is
T2248	4289-4296	JJ	denotes	crucial
T2249	4297-4300	IN	denotes	for
T2250	4301-4309	JJ	denotes	neuronal
T2251	4310-4320	NN	denotes	maturation
T2252	4321-4324	CC	denotes	and
T2253	4325-4332	NN	denotes	circuit
T2254	4333-4341	NN	denotes	assembly
T2255	4341-4342	.	denotes	.
T2468	4352-4354	TO	denotes	To
T2469	4355-4359	VB	denotes	test
T2471	4360-4363	DT	denotes	the
T2472	4364-4374	NN	denotes	hypothesis
T2473	4375-4379	IN	denotes	that
T2475	4380-4381	DT	denotes	a
T2477	4382-4390	JJ	denotes	temporal
T2476	4391-4396	NN	denotes	delay
T2478	4397-4399	IN	denotes	in
T2479	4400-4403	DT	denotes	the
T2480	4404-4409	NN	denotes	onset
T2481	4410-4412	IN	denotes	of
T2482	4413-4428	JJ	denotes	transcriptional
T2483	4429-4437	NNS	denotes	programs
T2474	4438-4440	VBZ	denotes	is
T2484	4441-4448	JJ	denotes	crucial
T2485	4449-4452	IN	denotes	for
T2486	4453-4456	DT	denotes	the
T2487	4457-4464	NN	denotes	control
T2488	4465-4467	IN	denotes	of
T2489	4468-4479	JJ	denotes	appropriate
T2491	4480-4488	JJ	denotes	neuronal
T2490	4489-4499	NN	denotes	maturation
T2492	4499-4501	,	denotes	,
T2493	4501-4503	PRP	denotes	we
T2470	4504-4511	VBD	denotes	studied
T2494	4512-4515	DT	denotes	the
T2495	4516-4527	NN	denotes	development
T2496	4528-4530	IN	denotes	of
T2497	4531-4545	JJ	denotes	proprioceptive
T2499	4546-4549	NN	denotes	DRG
T2498	4550-4557	NNS	denotes	neurons
T2500	4557-4559	,	denotes	,
T2501	4559-4564	IN	denotes	since
T2503	4565-4580	JJ	denotes	transcriptional
T2504	4581-4590	NNS	denotes	effectors
T2505	4591-4600	VBN	denotes	regulated
T2506	4601-4603	IN	denotes	by
T2507	4604-4610	NN	denotes	target
T2509	4610-4611	HYPH	denotes	-
T2508	4611-4618	VBN	denotes	derived
T2510	4619-4626	NNS	denotes	signals
T2511	4626-4628	,	denotes	,
T2512	4628-4630	RB	denotes	as
T2514	4631-4635	RB	denotes	well
T2513	4636-4638	IN	denotes	as
T2515	4639-4643	DT	denotes	some
T2516	4644-4646	IN	denotes	of
T2517	4647-4652	PRP$	denotes	their
T2519	4653-4663	JJ	denotes	downstream
T2520	4664-4674	JJ	denotes	biological
T2518	4675-4682	NNS	denotes	actions
T2521	4682-4684	,	denotes	,
T2522	4684-4688	VBP	denotes	have
T2523	4689-4693	VBN	denotes	been
T2502	4694-4704	VBN	denotes	identified
T2524	4705-4708	IN	denotes	for
T2525	4709-4714	DT	denotes	these
T2526	4715-4722	NNS	denotes	neurons
T2527	4722-4723	.	denotes	.
T2528	4723-5000	sentence	denotes	Er81 controls proprioceptive afferent connectivity [14], and we therefore sought to identify an ETS transcriptional regulator that, when expressed over the normal time course of Er81 expression, is able to substitute for Er81 function within group Ia afferent sensory neurons.
T2529	4724-4728	NN	denotes	Er81
T2530	4729-4737	VBZ	denotes	controls
T2531	4738-4752	JJ	denotes	proprioceptive
T2533	4753-4761	JJ	denotes	afferent
T2532	4762-4774	NN	denotes	connectivity
T2534	4775-4776	-LRB-	denotes	[
T2535	4776-4778	CD	denotes	14
T2536	4778-4779	-RRB-	denotes	]
T2537	4779-4781	,	denotes	,
T2538	4781-4784	CC	denotes	and
T2539	4785-4787	PRP	denotes	we
T2541	4788-4797	RB	denotes	therefore
T2540	4798-4804	VBD	denotes	sought
T2542	4805-4807	TO	denotes	to
T2543	4808-4816	VB	denotes	identify
T2544	4817-4819	DT	denotes	an
T2546	4820-4823	NN	denotes	ETS
T2547	4824-4839	JJ	denotes	transcriptional
T2545	4840-4849	NN	denotes	regulator
T2548	4850-4854	WDT	denotes	that
T2550	4854-4856	,	denotes	,
T2551	4856-4860	WRB	denotes	when
T2552	4861-4870	VBN	denotes	expressed
T2553	4871-4875	IN	denotes	over
T2554	4876-4879	DT	denotes	the
T2556	4880-4886	JJ	denotes	normal
T2557	4887-4891	NN	denotes	time
T2555	4892-4898	NN	denotes	course
T2558	4899-4901	IN	denotes	of
T2559	4902-4906	NN	denotes	Er81
T2560	4907-4917	NN	denotes	expression
T2561	4917-4919	,	denotes	,
T2549	4919-4921	VBZ	denotes	is
T2562	4922-4926	JJ	denotes	able
T2563	4927-4929	TO	denotes	to
T2564	4930-4940	VB	denotes	substitute
T2565	4941-4944	IN	denotes	for
T2566	4945-4949	NN	denotes	Er81
T2567	4950-4958	NN	denotes	function
T2568	4959-4965	IN	denotes	within
T2569	4966-4971	NN	denotes	group
T2570	4972-4974	NN	denotes	Ia
T2572	4975-4983	JJ	denotes	afferent
T2573	4984-4991	JJ	denotes	sensory
T2571	4992-4999	NNS	denotes	neurons
T2574	4999-5000	.	denotes	.
T2575	5000-5189	sentence	denotes	With this reference point, we then designed experiments to examine the effects of precocious post-mitotic expression of the same ETS transcription factor on sensory neuron differentiation.
T2576	5001-5005	IN	denotes	With
T2578	5006-5010	DT	denotes	this
T2580	5011-5020	NN	denotes	reference
T2579	5021-5026	NN	denotes	point
T2581	5026-5028	,	denotes	,
T2582	5028-5030	PRP	denotes	we
T2583	5031-5035	RB	denotes	then
T2577	5036-5044	VBD	denotes	designed
T2584	5045-5056	NNS	denotes	experiments
T2585	5057-5059	TO	denotes	to
T2586	5060-5067	VB	denotes	examine
T2587	5068-5071	DT	denotes	the
T2588	5072-5079	NNS	denotes	effects
T2589	5080-5082	IN	denotes	of
T2590	5083-5093	JJ	denotes	precocious
T2592	5094-5106	JJ	denotes	post-mitotic
T2591	5107-5117	NN	denotes	expression
T2593	5118-5120	IN	denotes	of
T2594	5121-5124	DT	denotes	the
T2596	5125-5129	JJ	denotes	same
T2597	5130-5133	NN	denotes	ETS
T2598	5134-5147	NN	denotes	transcription
T2595	5148-5154	NN	denotes	factor
T2599	5155-5157	IN	denotes	on
T2600	5158-5165	JJ	denotes	sensory
T2601	5166-5172	NN	denotes	neuron
T2602	5173-5188	NN	denotes	differentiation
T2603	5188-5189	.	denotes	.
T3609	5191-5194	NN	denotes	EWS
T3611	5194-5195	HYPH	denotes	-
T3610	5195-5199	NN	denotes	Pea3
T3613	5200-5203	MD	denotes	Can
T3612	5204-5211	VB	denotes	Replace
T3614	5212-5216	NN	denotes	Er81
T3615	5217-5225	NN	denotes	Function
T3616	5226-5228	IN	denotes	in
T3617	5229-5240	VBG	denotes	Controlling
T3618	5241-5243	NN	denotes	Ia
T3620	5244-5252	JJ	denotes	Afferent
T3619	5253-5264	NNS	denotes	Projections
T3621	5264-5441	sentence	denotes	We first defined an ETS transcription regulator that is able to replace the function of Er81 within proprioceptive afferents to direct projections into the ventral spinal cord.
T3622	5265-5267	PRP	denotes	We
T3624	5268-5273	RB	denotes	first
T3623	5274-5281	VBD	denotes	defined
T3625	5282-5284	DT	denotes	an
T3627	5285-5288	NN	denotes	ETS
T3628	5289-5302	NN	denotes	transcription
T3626	5303-5312	NN	denotes	regulator
T3629	5313-5317	WDT	denotes	that
T3630	5318-5320	VBZ	denotes	is
T3631	5321-5325	JJ	denotes	able
T3632	5326-5328	TO	denotes	to
T3633	5329-5336	VB	denotes	replace
T3634	5337-5340	DT	denotes	the
T3635	5341-5349	NN	denotes	function
T3636	5350-5352	IN	denotes	of
T3637	5353-5357	NN	denotes	Er81
T3638	5358-5364	IN	denotes	within
T3639	5365-5379	JJ	denotes	proprioceptive
T3640	5380-5389	NNS	denotes	afferents
T3641	5390-5392	TO	denotes	to
T3642	5393-5399	VB	denotes	direct
T3643	5400-5411	NNS	denotes	projections
T3644	5412-5416	IN	denotes	into
T3645	5417-5420	DT	denotes	the
T3647	5421-5428	JJ	denotes	ventral
T3648	5429-5435	JJ	denotes	spinal
T3646	5436-5440	NN	denotes	cord
T3649	5440-5441	.	denotes	.
T3650	5441-5622	sentence	denotes	Er81, Pea3, and Erm constitute the Pea3 subfamily of ETS transcription factors, show a high degree of amino acid identity, and bind to very similar DNA target sequences [17,18,19].
T3651	5442-5446	NN	denotes	Er81
T3653	5446-5448	,	denotes	,
T3654	5448-5452	NN	denotes	Pea3
T3655	5452-5454	,	denotes	,
T3656	5454-5457	CC	denotes	and
T3657	5458-5461	NN	denotes	Erm
T3652	5462-5472	VBP	denotes	constitute
T3658	5473-5476	DT	denotes	the
T3660	5477-5481	NN	denotes	Pea3
T3659	5482-5491	NN	denotes	subfamily
T3661	5492-5494	IN	denotes	of
T3662	5495-5498	NN	denotes	ETS
T3664	5499-5512	NN	denotes	transcription
T3663	5513-5520	NNS	denotes	factors
T3665	5520-5522	,	denotes	,
T3666	5522-5526	VBP	denotes	show
T3667	5527-5528	DT	denotes	a
T3669	5529-5533	JJ	denotes	high
T3668	5534-5540	NN	denotes	degree
T3670	5541-5543	IN	denotes	of
T3671	5544-5549	NN	denotes	amino
T3672	5550-5554	NN	denotes	acid
T3673	5555-5563	NN	denotes	identity
T3674	5563-5565	,	denotes	,
T3675	5565-5568	CC	denotes	and
T3676	5569-5573	VBP	denotes	bind
T3677	5574-5576	IN	denotes	to
T3678	5577-5581	RB	denotes	very
T3679	5582-5589	JJ	denotes	similar
T3681	5590-5593	NN	denotes	DNA
T3682	5594-5600	NN	denotes	target
T3680	5601-5610	NNS	denotes	sequences
T3683	5611-5612	-LRB-	denotes	[
T3685	5612-5614	CD	denotes	17
T3686	5614-5615	,	denotes	,
T3687	5615-5617	CD	denotes	18
T3688	5617-5618	,	denotes	,
T3684	5618-5620	CD	denotes	19
T3689	5620-5621	-RRB-	denotes	]
T3690	5621-5622	.	denotes	.
T3691	5622-5896	sentence	denotes	Nevertheless, when introduced into the Er81 locus in analogy to a previously used targeting strategy (data not shown; [14]), neither Pea3 nor Erm could rescue Ia proprioceptive afferent projections to extensively invade the ventral horn of the spinal cord (data not shown).
T3692	5623-5635	RB	denotes	Nevertheless
T3694	5635-5637	,	denotes	,
T3695	5637-5641	WRB	denotes	when
T3696	5642-5652	VBN	denotes	introduced
T3697	5653-5657	IN	denotes	into
T3698	5658-5661	DT	denotes	the
T3700	5662-5666	NN	denotes	Er81
T3699	5667-5672	NN	denotes	locus
T3701	5673-5675	IN	denotes	in
T3702	5676-5683	NN	denotes	analogy
T3703	5684-5686	IN	denotes	to
T3704	5687-5688	DT	denotes	a
T3706	5689-5699	RB	denotes	previously
T3707	5700-5704	VBN	denotes	used
T3708	5705-5714	NN	denotes	targeting
T3705	5715-5723	NN	denotes	strategy
T3709	5724-5725	-LRB-	denotes	(
T3711	5725-5729	NNS	denotes	data
T3712	5730-5733	RB	denotes	not
T3710	5734-5739	VBN	denotes	shown
T3713	5739-5740	:	denotes	;
T3714	5741-5742	-LRB-	denotes	[
T3715	5742-5744	CD	denotes	14
T3716	5744-5745	-RRB-	denotes	]
T3717	5745-5746	-RRB-	denotes	)
T3718	5746-5748	,	denotes	,
T3719	5748-5755	CC	denotes	neither
T3720	5756-5760	NN	denotes	Pea3
T3721	5761-5764	CC	denotes	nor
T3722	5765-5768	NN	denotes	Erm
T3723	5769-5774	MD	denotes	could
T3693	5775-5781	VB	denotes	rescue
T3724	5782-5784	NN	denotes	Ia
T3725	5785-5799	JJ	denotes	proprioceptive
T3727	5800-5808	JJ	denotes	afferent
T3726	5809-5820	NNS	denotes	projections
T3728	5821-5823	TO	denotes	to
T3730	5824-5835	RB	denotes	extensively
T3729	5836-5842	VB	denotes	invade
T3731	5843-5846	DT	denotes	the
T3733	5847-5854	JJ	denotes	ventral
T3732	5855-5859	NN	denotes	horn
T3734	5860-5862	IN	denotes	of
T3735	5863-5866	DT	denotes	the
T3737	5867-5873	JJ	denotes	spinal
T3736	5874-5878	NN	denotes	cord
T3738	5879-5880	-LRB-	denotes	(
T3740	5880-5884	NNS	denotes	data
T3741	5885-5888	RB	denotes	not
T3739	5889-5894	VBN	denotes	shown
T3742	5894-5895	-RRB-	denotes	)
T3743	5895-5896	.	denotes	.
T3744	5896-6140	sentence	denotes	These findings prompted us to analyze mice in which we integrated EWS-Pea3, a break-point fusion product between the amino-terminal domain of the Ewing sarcoma (EWS) gene and the Pea3 DNA binding domain [20,21], into the Er81 locus (Figure 1).
T3745	5897-5902	DT	denotes	These
T3746	5903-5911	NNS	denotes	findings
T3747	5912-5920	VBD	denotes	prompted
T3748	5921-5923	PRP	denotes	us
T3749	5924-5926	TO	denotes	to
T3750	5927-5934	VB	denotes	analyze
T3751	5935-5939	NNS	denotes	mice
T3752	5940-5942	IN	denotes	in
T3754	5943-5948	WDT	denotes	which
T3755	5949-5951	PRP	denotes	we
T3753	5952-5962	VBD	denotes	integrated
T3756	5963-5966	NN	denotes	EWS
T3758	5966-5967	HYPH	denotes	-
T3757	5967-5971	NN	denotes	Pea3
T3759	5971-5973	,	denotes	,
T3760	5973-5974	DT	denotes	a
T3762	5975-5980	NN	denotes	break
T3764	5980-5981	HYPH	denotes	-
T3763	5981-5986	NN	denotes	point
T3765	5987-5993	NN	denotes	fusion
T3761	5994-6001	NN	denotes	product
T3766	6002-6009	IN	denotes	between
T3767	6010-6013	DT	denotes	the
T3769	6014-6019	NN	denotes	amino
T3771	6019-6020	HYPH	denotes	-
T3770	6020-6028	JJ	denotes	terminal
T3768	6029-6035	NN	denotes	domain
T3772	6036-6038	IN	denotes	of
T3773	6039-6042	DT	denotes	the
T3775	6043-6048	NN	denotes	Ewing
T3776	6049-6056	NN	denotes	sarcoma
T3777	6057-6058	-LRB-	denotes	(
T3778	6058-6061	NN	denotes	EWS
T3779	6061-6062	-RRB-	denotes	)
T3774	6063-6067	NN	denotes	gene
T3780	6068-6071	CC	denotes	and
T3781	6072-6075	DT	denotes	the
T3783	6076-6080	NN	denotes	Pea3
T3784	6081-6084	NN	denotes	DNA
T3785	6085-6092	VBG	denotes	binding
T3782	6093-6099	NN	denotes	domain
T3786	6100-6101	-LRB-	denotes	[
T3788	6101-6103	CD	denotes	20
T3789	6103-6104	,	denotes	,
T3787	6104-6106	CD	denotes	21
T3790	6106-6107	-RRB-	denotes	]
T3791	6107-6109	,	denotes	,
T3792	6109-6113	IN	denotes	into
T3793	6114-6117	DT	denotes	the
T3795	6118-6122	NN	denotes	Er81
T3794	6123-6128	NN	denotes	locus
T3796	6129-6130	-LRB-	denotes	(
T3797	6130-6136	NN	denotes	Figure
T3798	6137-6138	CD	denotes	1
T3799	6138-6139	-RRB-	denotes	)
T3800	6139-6140	.	denotes	.
T3801	6140-6360	sentence	denotes	We found that in a luciferase-enzyme-based cell culture transfection assay, EWS-Pea3 showed stronger transactivation activity than Er81 or Pea3 (Figure 1J; data not shown), in agreement with previous studies [22,23,24].
T3802	6141-6143	PRP	denotes	We
T3803	6144-6149	VBD	denotes	found
T3804	6150-6154	IN	denotes	that
T3806	6155-6157	IN	denotes	in
T3807	6158-6159	DT	denotes	a
T3809	6160-6170	NN	denotes	luciferase
T3811	6170-6171	HYPH	denotes	-
T3810	6171-6177	NN	denotes	enzyme
T3813	6177-6178	HYPH	denotes	-
T3812	6178-6183	VBN	denotes	based
T3814	6184-6188	NN	denotes	cell
T3815	6189-6196	NN	denotes	culture
T3816	6197-6209	NN	denotes	transfection
T3808	6210-6215	NN	denotes	assay
T3817	6215-6217	,	denotes	,
T3818	6217-6220	NN	denotes	EWS
T3820	6220-6221	HYPH	denotes	-
T3819	6221-6225	NN	denotes	Pea3
T3805	6226-6232	VBD	denotes	showed
T3821	6233-6241	JJR	denotes	stronger
T3823	6242-6257	NN	denotes	transactivation
T3822	6258-6266	NN	denotes	activity
T3824	6267-6271	IN	denotes	than
T3825	6272-6276	NN	denotes	Er81
T3826	6277-6279	CC	denotes	or
T3827	6280-6284	NN	denotes	Pea3
T3828	6285-6286	-LRB-	denotes	(
T3830	6286-6292	NN	denotes	Figure
T3829	6293-6295	NN	denotes	1J
T3831	6295-6296	:	denotes	;
T3832	6297-6301	NNS	denotes	data
T3834	6302-6305	RB	denotes	not
T3833	6306-6311	VBN	denotes	shown
T3835	6311-6312	-RRB-	denotes	)
T3836	6312-6314	,	denotes	,
T3837	6314-6316	IN	denotes	in
T3838	6317-6326	NN	denotes	agreement
T3839	6327-6331	IN	denotes	with
T3840	6332-6340	JJ	denotes	previous
T3841	6341-6348	NNS	denotes	studies
T3842	6349-6350	-LRB-	denotes	[
T3844	6350-6352	CD	denotes	22
T3845	6352-6353	,	denotes	,
T3846	6353-6355	CD	denotes	23
T3847	6355-6356	,	denotes	,
T3843	6356-6358	CD	denotes	24
T3848	6358-6359	-RRB-	denotes	]
T3849	6359-6360	.	denotes	.
T3850	6360-6530	sentence	denotes	Moreover, transactivation by EWS-Pea3 was abolished by mutation of ETS-binding sites in the reporter plasmid, demonstrating ETS-binding-site dependence (data not shown).
T3851	6361-6369	RB	denotes	Moreover
T3853	6369-6371	,	denotes	,
T3854	6371-6386	NN	denotes	transactivation
T3855	6387-6389	IN	denotes	by
T3856	6390-6393	NN	denotes	EWS
T3858	6393-6394	HYPH	denotes	-
T3857	6394-6398	NN	denotes	Pea3
T3859	6399-6402	VBD	denotes	was
T3852	6403-6412	VBN	denotes	abolished
T3860	6413-6415	IN	denotes	by
T3861	6416-6424	NN	denotes	mutation
T3862	6425-6427	IN	denotes	of
T3863	6428-6431	NN	denotes	ETS
T3865	6431-6432	HYPH	denotes	-
T3864	6432-6439	VBG	denotes	binding
T3866	6440-6445	NNS	denotes	sites
T3867	6446-6448	IN	denotes	in
T3868	6449-6452	DT	denotes	the
T3870	6453-6461	NN	denotes	reporter
T3869	6462-6469	NN	denotes	plasmid
T3871	6469-6471	,	denotes	,
T3872	6471-6484	VBG	denotes	demonstrating
T3873	6485-6488	NN	denotes	ETS
T3875	6488-6489	HYPH	denotes	-
T3874	6489-6496	VBG	denotes	binding
T3877	6496-6497	HYPH	denotes	-
T3876	6497-6501	NN	denotes	site
T3878	6502-6512	NN	denotes	dependence
T3879	6513-6514	-LRB-	denotes	(
T3881	6514-6518	NNS	denotes	data
T3882	6519-6522	RB	denotes	not
T3880	6523-6528	VBN	denotes	shown
T3883	6528-6529	-RRB-	denotes	)
T3884	6529-6530	.	denotes	.
T3885	6530-8522	sentence	denotes	Figure 1 Replacement of Er81 by EWS-Pea3 (A) Generation of Er81EWS-Pea3 mutant mice. Above is the organization of the Er81 genomic locus in the region targeted by homologous recombination in analogy to [14]. Exons 1–4 are shown as light blue boxes, and the Er81 start codon in exon 2 is indicated as ATG. The probe used to detect homologous recombination is shown as a grey box. Below is replacement of Er81 by EWS-Pea3 through the integration of EWS-Pea3 in frame with the endogenous start codon of the Er81 locus in exon 2 (in analogy to [14]). (B) PCR and Southern blot analysis of Er81EWS-Pea3 wild-type (+/+), heterozygous (+/−), and homozygous (−/−) genomic DNA to detect the mutant allele. PCR primer pairs (EWS-Pea3ki) were used to detect specifically the recombined allele, and a primer pair in exon2 was used to detect the presence of the wild-type allele [14]. (C–E) Analysis of Er81 expression in lumbar DRG neurons of E16.5 wild-type (C), Er81−/− (D), and Er81EWS-Pea3/− (E) embryos. Inset in lower right corner of each panel shows Isl1 expression in the respective DRG. (F–H) PV expression in lumbar DRG of E16.5 wild-type (F), Er81−/− (G), and Er81EWS-Pea3/− (H) embryos. Confocal scans were performed with equal gain intensity. (J) Transcriptional transactivation of luciferase expression from a minimal reporter construct containing five consensus ETS DNA-binding sites ( GCCGGAAGC; [18,19]) and a minimal TK promoter upon transient transfection of Er81 (n ≥ 7; 3.03 ± 0.66) or EWS-Pea3 (n ≥ 7; 20.3 ± 2.7). Relative luciferase activity normalized to control (Con). Scale bar: 80 μm. Expression of Er81 in DRG neurons of embryos containing integration of EWS-Pea3 in the Er81 locus (Er81EWS-Pea3/−) was abolished (Figure 1E), and the expression level of the calcium-binding protein Parvalbumin (PV) in proprioceptive afferents, which is decreased approximately 5- to 10-fold in Er81 mutants [14], was comparable to wild-type levels in Er81EWS-Pea3/− embryos (Figure 1F–1H).
T3886	8133-8143	NN	denotes	Expression
T3888	8144-8146	IN	denotes	of
T3889	8147-8151	NN	denotes	Er81
T3890	8152-8154	IN	denotes	in
T3891	8155-8158	NN	denotes	DRG
T3892	8159-8166	NNS	denotes	neurons
T3893	8167-8169	IN	denotes	of
T3894	8170-8177	NNS	denotes	embryos
T3895	8178-8188	VBG	denotes	containing
T3896	8189-8200	NN	denotes	integration
T3897	8201-8203	IN	denotes	of
T3898	8204-8207	NN	denotes	EWS
T3900	8207-8208	HYPH	denotes	-
T3899	8208-8212	NN	denotes	Pea3
T3901	8213-8215	IN	denotes	in
T3902	8216-8219	DT	denotes	the
T3904	8220-8224	NN	denotes	Er81
T3903	8225-8230	NN	denotes	locus
T3905	8231-8232	-LRB-	denotes	(
T3906	8232-8239	NN	denotes	Er81EWS
T3908	8239-8240	HYPH	denotes	-
T3907	8240-8244	NN	denotes	Pea3
T3909	8244-8245	HYPH	denotes	/
T3910	8245-8246	SYM	denotes	−
T3911	8246-8247	-RRB-	denotes	)
T3912	8248-8251	VBD	denotes	was
T3887	8252-8261	VBN	denotes	abolished
T3913	8262-8263	-LRB-	denotes	(
T3915	8263-8269	NN	denotes	Figure
T3914	8270-8272	NN	denotes	1E
T3916	8272-8273	-RRB-	denotes	)
T3917	8273-8275	,	denotes	,
T3918	8275-8278	CC	denotes	and
T3919	8279-8282	DT	denotes	the
T3921	8283-8293	NN	denotes	expression
T3920	8294-8299	NN	denotes	level
T3923	8300-8302	IN	denotes	of
T3924	8303-8306	DT	denotes	the
T3926	8307-8314	NN	denotes	calcium
T3928	8314-8315	HYPH	denotes	-
T3927	8315-8322	VBG	denotes	binding
T3925	8323-8330	NN	denotes	protein
T3929	8331-8342	NN	denotes	Parvalbumin
T3930	8343-8344	-LRB-	denotes	(
T3931	8344-8346	NN	denotes	PV
T3932	8346-8347	-RRB-	denotes	)
T3933	8348-8350	IN	denotes	in
T3934	8351-8365	JJ	denotes	proprioceptive
T3935	8366-8375	NNS	denotes	afferents
T3936	8375-8377	,	denotes	,
T3937	8377-8382	WDT	denotes	which
T3939	8383-8385	VBZ	denotes	is
T3938	8386-8395	VBN	denotes	decreased
T3940	8396-8409	RB	denotes	approximately
T3942	8410-8411	CD	denotes	5
T3943	8411-8412	HYPH	denotes	-
T3944	8413-8415	IN	denotes	to
T3941	8416-8418	CD	denotes	10
T3946	8418-8419	HYPH	denotes	-
T3945	8419-8423	RB	denotes	fold
T3947	8424-8426	IN	denotes	in
T3948	8427-8431	NN	denotes	Er81
T3949	8432-8439	NNS	denotes	mutants
T3950	8440-8441	-LRB-	denotes	[
T3951	8441-8443	CD	denotes	14
T3952	8443-8444	-RRB-	denotes	]
T3953	8444-8446	,	denotes	,
T3922	8446-8449	VBD	denotes	was
T3954	8450-8460	JJ	denotes	comparable
T3955	8461-8463	IN	denotes	to
T3956	8464-8468	JJ	denotes	wild
T3958	8468-8469	HYPH	denotes	-
T3957	8469-8473	NN	denotes	type
T3959	8474-8480	NNS	denotes	levels
T3960	8481-8483	IN	denotes	in
T3961	8484-8491	NN	denotes	Er81EWS
T3963	8491-8492	HYPH	denotes	-
T3962	8492-8496	NN	denotes	Pea3
T3965	8496-8497	HYPH	denotes	/
T3966	8497-8498	SYM	denotes	−
T3964	8499-8506	NNS	denotes	embryos
T3967	8507-8508	-LRB-	denotes	(
T3969	8508-8514	NN	denotes	Figure
T3968	8515-8517	NN	denotes	1F
T3970	8517-8518	SYM	denotes	–
T3971	8518-8520	NN	denotes	1H
T3972	8520-8521	-RRB-	denotes	)
T3973	8521-8522	.	denotes	.
T3974	8522-8786	sentence	denotes	To further define DRG neuron differentiation in the presence of EWS-Pea3 in proprioceptive afferents in vivo, we assessed whether replacement of Er81 by EWS-Pea3 had an influence on neuronal survival or on the expression of proprioceptive-afferent-specific genes.
T3975	8523-8525	TO	denotes	To
T3977	8526-8533	RB	denotes	further
T3976	8534-8540	VB	denotes	define
T3979	8541-8544	NN	denotes	DRG
T3980	8545-8551	NN	denotes	neuron
T3981	8552-8567	NN	denotes	differentiation
T3982	8568-8570	IN	denotes	in
T3983	8571-8574	DT	denotes	the
T3984	8575-8583	NN	denotes	presence
T3985	8584-8586	IN	denotes	of
T3986	8587-8590	NN	denotes	EWS
T3988	8590-8591	HYPH	denotes	-
T3987	8591-8595	NN	denotes	Pea3
T3989	8596-8598	IN	denotes	in
T3990	8599-8613	JJ	denotes	proprioceptive
T3991	8614-8623	NNS	denotes	afferents
T3992	8624-8626	FW	denotes	in
T3993	8627-8631	FW	denotes	vivo
T3994	8631-8633	,	denotes	,
T3995	8633-8635	PRP	denotes	we
T3978	8636-8644	VBD	denotes	assessed
T3996	8645-8652	IN	denotes	whether
T3998	8653-8664	NN	denotes	replacement
T3999	8665-8667	IN	denotes	of
T4000	8668-8672	NN	denotes	Er81
T4001	8673-8675	IN	denotes	by
T4002	8676-8679	NN	denotes	EWS
T4004	8679-8680	HYPH	denotes	-
T4003	8680-8684	NN	denotes	Pea3
T3997	8685-8688	VBD	denotes	had
T4005	8689-8691	DT	denotes	an
T4006	8692-8701	NN	denotes	influence
T4007	8702-8704	IN	denotes	on
T4008	8705-8713	JJ	denotes	neuronal
T4009	8714-8722	NN	denotes	survival
T4010	8723-8725	CC	denotes	or
T4011	8726-8728	IN	denotes	on
T4012	8729-8732	DT	denotes	the
T4013	8733-8743	NN	denotes	expression
T4014	8744-8746	IN	denotes	of
T4015	8747-8761	JJ	denotes	proprioceptive
T4017	8761-8762	HYPH	denotes	-
T4016	8762-8770	JJ	denotes	afferent
T4019	8770-8771	HYPH	denotes	-
T4018	8771-8779	JJ	denotes	specific
T4020	8780-8785	NNS	denotes	genes
T4021	8785-8786	.	denotes	.
T4022	8786-9113	sentence	denotes	Er81EWS-Pea3/− mice did not differ from wild-type in the number of proprioceptive afferent cell bodies within the DRG of L1 to L5, the expression of several genes normally expressed by proprioceptive afferents, and the lack of expression of genes not normally expressed in proprioceptive afferents (Figure S1; data not shown).
T4023	8787-8794	NN	denotes	Er81EWS
T4025	8794-8795	HYPH	denotes	-
T4024	8795-8799	NN	denotes	Pea3
T4027	8799-8800	HYPH	denotes	/
T4028	8800-8801	SYM	denotes	−
T4026	8802-8806	NNS	denotes	mice
T4030	8807-8810	VBD	denotes	did
T4031	8811-8814	RB	denotes	not
T4029	8815-8821	VB	denotes	differ
T4032	8822-8826	IN	denotes	from
T4033	8827-8831	JJ	denotes	wild
T4035	8831-8832	HYPH	denotes	-
T4034	8832-8836	NN	denotes	type
T4036	8837-8839	IN	denotes	in
T4037	8840-8843	DT	denotes	the
T4038	8844-8850	NN	denotes	number
T4039	8851-8853	IN	denotes	of
T4040	8854-8868	JJ	denotes	proprioceptive
T4042	8869-8877	JJ	denotes	afferent
T4043	8878-8882	NN	denotes	cell
T4041	8883-8889	NNS	denotes	bodies
T4044	8890-8896	IN	denotes	within
T4045	8897-8900	DT	denotes	the
T4046	8901-8904	NN	denotes	DRG
T4047	8905-8907	IN	denotes	of
T4048	8908-8910	NN	denotes	L1
T4049	8911-8913	IN	denotes	to
T4050	8914-8916	NN	denotes	L5
T4051	8916-8918	,	denotes	,
T4052	8918-8921	DT	denotes	the
T4053	8922-8932	NN	denotes	expression
T4054	8933-8935	IN	denotes	of
T4055	8936-8943	JJ	denotes	several
T4056	8944-8949	NNS	denotes	genes
T4057	8950-8958	RB	denotes	normally
T4058	8959-8968	VBN	denotes	expressed
T4059	8969-8971	IN	denotes	by
T4060	8972-8986	JJ	denotes	proprioceptive
T4061	8987-8996	NNS	denotes	afferents
T4062	8996-8998	,	denotes	,
T4063	8998-9001	CC	denotes	and
T4064	9002-9005	DT	denotes	the
T4065	9006-9010	NN	denotes	lack
T4066	9011-9013	IN	denotes	of
T4067	9014-9024	NN	denotes	expression
T4068	9025-9027	IN	denotes	of
T4069	9028-9033	NNS	denotes	genes
T4070	9034-9037	RB	denotes	not
T4072	9038-9046	RB	denotes	normally
T4071	9047-9056	VBN	denotes	expressed
T4073	9057-9059	IN	denotes	in
T4074	9060-9074	JJ	denotes	proprioceptive
T4075	9075-9084	NNS	denotes	afferents
T4076	9085-9086	-LRB-	denotes	(
T4078	9086-9092	NN	denotes	Figure
T4077	9093-9095	NN	denotes	S1
T4079	9095-9096	:	denotes	;
T4080	9097-9101	NNS	denotes	data
T4082	9102-9105	RB	denotes	not
T4081	9106-9111	VBN	denotes	shown
T4083	9111-9112	-RRB-	denotes	)
T4084	9112-9113	.	denotes	.
T4085	9113-9329	sentence	denotes	Together, these findings suggest that the expression of EWS-Pea3 from the normal time of onset mimics the function of Er81 as assessed by induction and maintenance of gene expression within proprioceptive afferents.
T4086	9114-9122	RB	denotes	Together
T4088	9122-9124	,	denotes	,
T4089	9124-9129	DT	denotes	these
T4090	9130-9138	NNS	denotes	findings
T4087	9139-9146	VBP	denotes	suggest
T4091	9147-9151	IN	denotes	that
T4093	9152-9155	DT	denotes	the
T4094	9156-9166	NN	denotes	expression
T4095	9167-9169	IN	denotes	of
T4096	9170-9173	NN	denotes	EWS
T4098	9173-9174	HYPH	denotes	-
T4097	9174-9178	NN	denotes	Pea3
T4099	9179-9183	IN	denotes	from
T4100	9184-9187	DT	denotes	the
T4102	9188-9194	JJ	denotes	normal
T4101	9195-9199	NN	denotes	time
T4103	9200-9202	IN	denotes	of
T4104	9203-9208	NN	denotes	onset
T4092	9209-9215	VBZ	denotes	mimics
T4105	9216-9219	DT	denotes	the
T4106	9220-9228	NN	denotes	function
T4107	9229-9231	IN	denotes	of
T4108	9232-9236	NN	denotes	Er81
T4109	9237-9239	IN	denotes	as
T4110	9240-9248	VBN	denotes	assessed
T4111	9249-9251	IN	denotes	by
T4112	9252-9261	NN	denotes	induction
T4113	9262-9265	CC	denotes	and
T4114	9266-9277	NN	denotes	maintenance
T4115	9278-9280	IN	denotes	of
T4116	9281-9285	NN	denotes	gene
T4117	9286-9296	NN	denotes	expression
T4118	9297-9303	IN	denotes	within
T4119	9304-9318	JJ	denotes	proprioceptive
T4120	9319-9328	NNS	denotes	afferents
T4121	9328-9329	.	denotes	.
T4122	9329-9574	sentence	denotes	To determine the extent of rescue of Ia proprioceptive afferent projections into the ventral spinal cord of Er81 mutant mice achieved by expression of EWS-Pea3, we traced intraspinal afferent projections by axonal labeling of PV (Figure 2A–2C).
T4123	9330-9332	TO	denotes	To
T4124	9333-9342	VB	denotes	determine
T4126	9343-9346	DT	denotes	the
T4127	9347-9353	NN	denotes	extent
T4128	9354-9356	IN	denotes	of
T4129	9357-9363	NN	denotes	rescue
T4130	9364-9366	IN	denotes	of
T4131	9367-9369	NN	denotes	Ia
T4133	9370-9384	JJ	denotes	proprioceptive
T4134	9385-9393	JJ	denotes	afferent
T4132	9394-9405	NNS	denotes	projections
T4135	9406-9410	IN	denotes	into
T4136	9411-9414	DT	denotes	the
T4138	9415-9422	JJ	denotes	ventral
T4139	9423-9429	JJ	denotes	spinal
T4137	9430-9434	NN	denotes	cord
T4140	9435-9437	IN	denotes	of
T4141	9438-9442	NN	denotes	Er81
T4143	9443-9449	NN	denotes	mutant
T4142	9450-9454	NNS	denotes	mice
T4144	9455-9463	VBN	denotes	achieved
T4145	9464-9466	IN	denotes	by
T4146	9467-9477	NN	denotes	expression
T4147	9478-9480	IN	denotes	of
T4148	9481-9484	NN	denotes	EWS
T4150	9484-9485	HYPH	denotes	-
T4149	9485-9489	NN	denotes	Pea3
T4151	9489-9491	,	denotes	,
T4152	9491-9493	PRP	denotes	we
T4125	9494-9500	VBD	denotes	traced
T4153	9501-9512	JJ	denotes	intraspinal
T4155	9513-9521	JJ	denotes	afferent
T4154	9522-9533	NNS	denotes	projections
T4156	9534-9536	IN	denotes	by
T4157	9537-9543	JJ	denotes	axonal
T4158	9544-9552	NN	denotes	labeling
T4159	9553-9555	IN	denotes	of
T4160	9556-9558	NN	denotes	PV
T4161	9559-9560	-LRB-	denotes	(
T4163	9560-9566	NN	denotes	Figure
T4162	9567-9569	NN	denotes	2A
T4164	9569-9570	SYM	denotes	–
T4165	9570-9572	NN	denotes	2C
T4166	9572-9573	-RRB-	denotes	)
T4167	9573-9574	.	denotes	.
T4168	9574-9796	sentence	denotes	In addition, to analyze axon ingrowth independent of the level of PV expression in DRG neurons, we used anterograde labeling of afferent fibers by applying fluorescently labeled dextran to cut dorsal roots (Figure 2D–2F).
T4169	9575-9577	IN	denotes	In
T4171	9578-9586	NN	denotes	addition
T4172	9586-9588	,	denotes	,
T4173	9588-9590	TO	denotes	to
T4174	9591-9598	VB	denotes	analyze
T4175	9599-9603	NN	denotes	axon
T4176	9604-9612	NN	denotes	ingrowth
T4177	9613-9624	RB	denotes	independent
T4178	9625-9627	IN	denotes	of
T4179	9628-9631	DT	denotes	the
T4180	9632-9637	NN	denotes	level
T4181	9638-9640	IN	denotes	of
T4182	9641-9643	NN	denotes	PV
T4183	9644-9654	NN	denotes	expression
T4184	9655-9657	IN	denotes	in
T4185	9658-9661	NN	denotes	DRG
T4186	9662-9669	NNS	denotes	neurons
T4187	9669-9671	,	denotes	,
T4188	9671-9673	PRP	denotes	we
T4170	9674-9678	VBD	denotes	used
T4189	9679-9690	JJ	denotes	anterograde
T4190	9691-9699	NN	denotes	labeling
T4191	9700-9702	IN	denotes	of
T4192	9703-9711	JJ	denotes	afferent
T4193	9712-9718	NNS	denotes	fibers
T4194	9719-9721	IN	denotes	by
T4195	9722-9730	VBG	denotes	applying
T4196	9731-9744	RB	denotes	fluorescently
T4197	9745-9752	VBN	denotes	labeled
T4198	9753-9760	NN	denotes	dextran
T4199	9761-9763	IN	denotes	to
T4200	9764-9767	VBN	denotes	cut
T4202	9768-9774	JJ	denotes	dorsal
T4201	9775-9780	NNS	denotes	roots
T4203	9781-9782	-LRB-	denotes	(
T4205	9782-9788	NN	denotes	Figure
T4204	9789-9791	NN	denotes	2D
T4206	9791-9792	SYM	denotes	–
T4207	9792-9794	NN	denotes	2F
T4208	9794-9795	-RRB-	denotes	)
T4209	9795-9796	.	denotes	.
T4210	9796-9944	sentence	denotes	Using both assays, we found extensive rescue of the projections into the ventral horn of the spinal cord in Er81EWS-Pea3/− mice (Figure 2C and 2F).
T4211	9797-9802	VBG	denotes	Using
T4213	9803-9807	DT	denotes	both
T4214	9808-9814	NNS	denotes	assays
T4215	9814-9816	,	denotes	,
T4216	9816-9818	PRP	denotes	we
T4212	9819-9824	VBD	denotes	found
T4217	9825-9834	JJ	denotes	extensive
T4218	9835-9841	NN	denotes	rescue
T4219	9842-9844	IN	denotes	of
T4220	9845-9848	DT	denotes	the
T4221	9849-9860	NNS	denotes	projections
T4222	9861-9865	IN	denotes	into
T4223	9866-9869	DT	denotes	the
T4225	9870-9877	JJ	denotes	ventral
T4224	9878-9882	NN	denotes	horn
T4226	9883-9885	IN	denotes	of
T4227	9886-9889	DT	denotes	the
T4229	9890-9896	JJ	denotes	spinal
T4228	9897-9901	NN	denotes	cord
T4230	9902-9904	IN	denotes	in
T4231	9905-9912	NN	denotes	Er81EWS
T4233	9912-9913	HYPH	denotes	-
T4232	9913-9917	NN	denotes	Pea3
T4235	9917-9918	HYPH	denotes	/
T4236	9918-9919	SYM	denotes	−
T4234	9920-9924	NNS	denotes	mice
T4237	9925-9926	-LRB-	denotes	(
T4239	9926-9932	NN	denotes	Figure
T4238	9933-9935	NN	denotes	2C
T4240	9936-9939	CC	denotes	and
T4241	9940-9942	NN	denotes	2F
T4242	9942-9943	-RRB-	denotes	)
T4243	9943-9944	.	denotes	.
T4244	9944-10102	sentence	denotes	Within the ventral horn, Ia afferents in both wild-type and Er81EWS-Pea3/− mice formed vGlut1+ terminals that were absent in Er81 mutant mice (Figure 2G–2I).
T4245	9945-9951	IN	denotes	Within
T4247	9952-9955	DT	denotes	the
T4249	9956-9963	JJ	denotes	ventral
T4248	9964-9968	NN	denotes	horn
T4250	9968-9970	,	denotes	,
T4251	9970-9972	NN	denotes	Ia
T4252	9973-9982	NNS	denotes	afferents
T4253	9983-9985	IN	denotes	in
T4254	9986-9990	CC	denotes	both
T4256	9991-9995	JJ	denotes	wild
T4257	9995-9996	HYPH	denotes	-
T4255	9996-10000	NN	denotes	type
T4259	10001-10004	CC	denotes	and
T4260	10005-10012	NN	denotes	Er81EWS
T4262	10012-10013	HYPH	denotes	-
T4261	10013-10017	NN	denotes	Pea3
T4263	10017-10018	HYPH	denotes	/
T4264	10018-10019	SYM	denotes	−
T4258	10020-10024	NNS	denotes	mice
T4246	10025-10031	VBD	denotes	formed
T4265	10032-10038	NN	denotes	vGlut1
T4267	10038-10039	SYM	denotes	+
T4266	10040-10049	NNS	denotes	terminals
T4268	10050-10054	WDT	denotes	that
T4269	10055-10059	VBD	denotes	were
T4270	10060-10066	JJ	denotes	absent
T4271	10067-10069	IN	denotes	in
T4272	10070-10074	NN	denotes	Er81
T4274	10075-10081	NN	denotes	mutant
T4273	10082-10086	NNS	denotes	mice
T4275	10087-10088	-LRB-	denotes	(
T4277	10088-10094	NN	denotes	Figure
T4276	10095-10097	NN	denotes	2G
T4278	10097-10098	SYM	denotes	–
T4279	10098-10100	NN	denotes	2I
T4280	10100-10101	-RRB-	denotes	)
T4281	10101-10102	.	denotes	.
T4282	10102-10355	sentence	denotes	To assess whether synapses between Ia afferents and motor neurons are functional in Er81EWS-Pea3/− mice, we performed intracellular recordings from identified quadriceps motor neurons after stimulation of nerves innervating the quadriceps muscle group.
T4283	10103-10105	TO	denotes	To
T4284	10106-10112	VB	denotes	assess
T4286	10113-10120	IN	denotes	whether
T4288	10121-10129	NNS	denotes	synapses
T4289	10130-10137	IN	denotes	between
T4290	10138-10140	NN	denotes	Ia
T4291	10141-10150	NNS	denotes	afferents
T4292	10151-10154	CC	denotes	and
T4293	10155-10160	NN	denotes	motor
T4294	10161-10168	NNS	denotes	neurons
T4287	10169-10172	VBP	denotes	are
T4295	10173-10183	JJ	denotes	functional
T4296	10184-10186	IN	denotes	in
T4297	10187-10194	NN	denotes	Er81EWS
T4299	10194-10195	HYPH	denotes	-
T4298	10195-10199	NN	denotes	Pea3
T4301	10199-10200	HYPH	denotes	/
T4302	10200-10201	SYM	denotes	−
T4300	10202-10206	NNS	denotes	mice
T4303	10206-10208	,	denotes	,
T4304	10208-10210	PRP	denotes	we
T4285	10211-10220	VBD	denotes	performed
T4305	10221-10234	JJ	denotes	intracellular
T4306	10235-10245	NNS	denotes	recordings
T4307	10246-10250	IN	denotes	from
T4308	10251-10261	VBN	denotes	identified
T4310	10262-10272	NNS	denotes	quadriceps
T4311	10273-10278	NN	denotes	motor
T4309	10279-10286	NNS	denotes	neurons
T4312	10287-10292	IN	denotes	after
T4313	10293-10304	NN	denotes	stimulation
T4314	10305-10307	IN	denotes	of
T4315	10308-10314	NNS	denotes	nerves
T4316	10315-10326	VBG	denotes	innervating
T4317	10327-10330	DT	denotes	the
T4319	10331-10341	NNS	denotes	quadriceps
T4320	10342-10348	NN	denotes	muscle
T4318	10349-10354	NN	denotes	group
T4321	10354-10355	.	denotes	.
T4322	10355-10572	sentence	denotes	We found no significant difference in the input amplitude to quadriceps motor neurons when comparing wild-type to Er81EWS-Pea3/− mice (Figure S2; wild-type, 10.6 ± 0.9 mV, n = 11; Er81EWS-Pea3/−, 10.9 ± 1 mV, n = 8).
T4323	10356-10358	PRP	denotes	We
T4324	10359-10364	VBD	denotes	found
T4325	10365-10367	DT	denotes	no
T4327	10368-10379	JJ	denotes	significant
T4326	10380-10390	NN	denotes	difference
T4328	10391-10393	IN	denotes	in
T4329	10394-10397	DT	denotes	the
T4331	10398-10403	NN	denotes	input
T4330	10404-10413	NN	denotes	amplitude
T4332	10414-10416	IN	denotes	to
T4333	10417-10427	NNS	denotes	quadriceps
T4335	10428-10433	NN	denotes	motor
T4334	10434-10441	NNS	denotes	neurons
T4336	10442-10446	WRB	denotes	when
T4337	10447-10456	VBG	denotes	comparing
T4338	10457-10461	JJ	denotes	wild
T4340	10461-10462	HYPH	denotes	-
T4339	10462-10466	NN	denotes	type
T4341	10467-10469	IN	denotes	to
T4342	10470-10477	NN	denotes	Er81EWS
T4344	10477-10478	HYPH	denotes	-
T4343	10478-10482	NN	denotes	Pea3
T4345	10482-10483	HYPH	denotes	/
T4346	10483-10484	SYM	denotes	−
T4347	10485-10489	NNS	denotes	mice
T4348	10490-10491	-LRB-	denotes	(
T4350	10491-10497	NN	denotes	Figure
T4349	10498-10500	NN	denotes	S2
T4351	10500-10501	:	denotes	;
T4352	10502-10506	JJ	denotes	wild
T4354	10506-10507	HYPH	denotes	-
T4353	10507-10511	NN	denotes	type
T4356	10511-10513	,	denotes	,
T4357	10513-10517	CD	denotes	10.6
T4359	10518-10519	SYM	denotes	±
T4358	10520-10523	CD	denotes	0.9
T4360	10524-10526	NN	denotes	mV
T4361	10526-10528	,	denotes	,
T4362	10528-10529	NN	denotes	n
T4363	10530-10531	SYM	denotes	=
T4355	10532-10534	CD	denotes	11
T4364	10534-10535	:	denotes	;
T4365	10536-10543	NN	denotes	Er81EWS
T4367	10543-10544	HYPH	denotes	-
T4366	10544-10548	NN	denotes	Pea3
T4369	10548-10549	HYPH	denotes	/
T4370	10549-10550	SYM	denotes	−
T4371	10550-10552	,	denotes	,
T4372	10552-10556	CD	denotes	10.9
T4374	10557-10558	SYM	denotes	±
T4373	10559-10560	CD	denotes	1
T4375	10561-10563	NN	denotes	mV
T4376	10563-10565	,	denotes	,
T4377	10565-10566	NN	denotes	n
T4378	10567-10568	SYM	denotes	=
T4368	10569-10570	CD	denotes	8
T4379	10570-10571	-RRB-	denotes	)
T4380	10571-10572	.	denotes	.
T4381	10572-10923	sentence	denotes	Together, these findings suggest that in the absence of Er81, EWS-Pea3 can direct the complex biological process of correct laminar termination within the ventral spinal cord and the formation of synapses with motor neurons (Figure 2J–2L), thus identifying an ETS transcription factor suitable for heterochronic expression experiments in DRG neurons.
T4382	10573-10581	RB	denotes	Together
T4384	10581-10583	,	denotes	,
T4385	10583-10588	DT	denotes	these
T4386	10589-10597	NNS	denotes	findings
T4383	10598-10605	VBP	denotes	suggest
T4387	10606-10610	IN	denotes	that
T4389	10611-10613	IN	denotes	in
T4390	10614-10617	DT	denotes	the
T4391	10618-10625	NN	denotes	absence
T4392	10626-10628	IN	denotes	of
T4393	10629-10633	NN	denotes	Er81
T4394	10633-10635	,	denotes	,
T4395	10635-10638	NN	denotes	EWS
T4397	10638-10639	HYPH	denotes	-
T4396	10639-10643	NN	denotes	Pea3
T4398	10644-10647	MD	denotes	can
T4388	10648-10654	VB	denotes	direct
T4399	10655-10658	DT	denotes	the
T4401	10659-10666	JJ	denotes	complex
T4402	10667-10677	JJ	denotes	biological
T4400	10678-10685	NN	denotes	process
T4403	10686-10688	IN	denotes	of
T4404	10689-10696	JJ	denotes	correct
T4406	10697-10704	JJ	denotes	laminar
T4405	10705-10716	NN	denotes	termination
T4407	10717-10723	IN	denotes	within
T4408	10724-10727	DT	denotes	the
T4410	10728-10735	JJ	denotes	ventral
T4411	10736-10742	JJ	denotes	spinal
T4409	10743-10747	NN	denotes	cord
T4412	10748-10751	CC	denotes	and
T4413	10752-10755	DT	denotes	the
T4414	10756-10765	NN	denotes	formation
T4415	10766-10768	IN	denotes	of
T4416	10769-10777	NNS	denotes	synapses
T4417	10778-10782	IN	denotes	with
T4418	10783-10788	NN	denotes	motor
T4419	10789-10796	NNS	denotes	neurons
T4420	10797-10798	-LRB-	denotes	(
T4422	10798-10804	NN	denotes	Figure
T4421	10805-10807	NN	denotes	2J
T4423	10807-10808	SYM	denotes	–
T4424	10808-10810	NN	denotes	2L
T4425	10810-10811	-RRB-	denotes	)
T4426	10811-10813	,	denotes	,
T4427	10813-10817	RB	denotes	thus
T4428	10818-10829	VBG	denotes	identifying
T4429	10830-10832	DT	denotes	an
T4431	10833-10836	NN	denotes	ETS
T4432	10837-10850	NN	denotes	transcription
T4430	10851-10857	NN	denotes	factor
T4433	10858-10866	JJ	denotes	suitable
T4434	10867-10870	IN	denotes	for
T4435	10871-10884	JJ	denotes	heterochronic
T4437	10885-10895	NN	denotes	expression
T4436	10896-10907	NNS	denotes	experiments
T4438	10908-10910	IN	denotes	in
T4439	10911-10914	NN	denotes	DRG
T4440	10915-10922	NNS	denotes	neurons
T4441	10922-10923	.	denotes	.
T5254	11936-11946	JJ	denotes	Precocious
T5255	11947-11957	NN	denotes	Expression
T5257	11958-11960	IN	denotes	of
T5258	11961-11964	NN	denotes	EWS
T5260	11964-11965	HYPH	denotes	-
T5259	11965-11969	NN	denotes	Pea3
T5261	11970-11972	IN	denotes	in
T5262	11973-11976	NN	denotes	DRG
T5263	11977-11984	NNS	denotes	Neurons
T5256	11985-11990	VBZ	denotes	Leads
T5264	11991-11993	IN	denotes	to
T5265	11994-12000	JJ	denotes	Axonal
T5267	12001-12011	NN	denotes	Projection
T5266	12012-12019	NNS	denotes	Defects
T5268	12019-12199	sentence	denotes	To address the consequences of precocious ETS signaling for proprioceptive afferent differentiation, we next expressed EWS-Pea3 in DRG neurons as soon as they became post-mitotic.
T5269	12020-12022	TO	denotes	To
T5270	12023-12030	VB	denotes	address
T5272	12031-12034	DT	denotes	the
T5273	12035-12047	NNS	denotes	consequences
T5274	12048-12050	IN	denotes	of
T5275	12051-12061	JJ	denotes	precocious
T5277	12062-12065	NN	denotes	ETS
T5276	12066-12075	NN	denotes	signaling
T5278	12076-12079	IN	denotes	for
T5279	12080-12094	JJ	denotes	proprioceptive
T5280	12095-12103	JJ	denotes	afferent
T5281	12104-12119	NN	denotes	differentiation
T5282	12119-12121	,	denotes	,
T5283	12121-12123	PRP	denotes	we
T5284	12124-12128	RB	denotes	next
T5271	12129-12138	VBD	denotes	expressed
T5285	12139-12142	NN	denotes	EWS
T5287	12142-12143	HYPH	denotes	-
T5286	12143-12147	NN	denotes	Pea3
T5288	12148-12150	IN	denotes	in
T5289	12151-12154	NN	denotes	DRG
T5290	12155-12162	NNS	denotes	neurons
T5291	12163-12165	RB	denotes	as
T5292	12166-12170	RB	denotes	soon
T5293	12171-12173	IN	denotes	as
T5295	12174-12178	PRP	denotes	they
T5294	12179-12185	VBD	denotes	became
T5296	12186-12198	JJ	denotes	post-mitotic
T5297	12198-12199	.	denotes	.
T5298	12199-12338	sentence	denotes	We used a binary mouse genetic system based on Cre-recombinase-mediated excision of a transcriptional stop cassette flanked by loxP sites.
T5299	12200-12202	PRP	denotes	We
T5300	12203-12207	VBD	denotes	used
T5301	12208-12209	DT	denotes	a
T5303	12210-12216	JJ	denotes	binary
T5304	12217-12222	NN	denotes	mouse
T5305	12223-12230	JJ	denotes	genetic
T5302	12231-12237	NN	denotes	system
T5306	12238-12243	VBN	denotes	based
T5307	12244-12246	IN	denotes	on
T5308	12247-12250	NN	denotes	Cre
T5310	12250-12251	HYPH	denotes	-
T5309	12251-12262	NN	denotes	recombinase
T5312	12262-12263	HYPH	denotes	-
T5311	12263-12271	VBN	denotes	mediated
T5313	12272-12280	NN	denotes	excision
T5314	12281-12283	IN	denotes	of
T5315	12284-12285	DT	denotes	a
T5317	12286-12301	JJ	denotes	transcriptional
T5318	12302-12306	NN	denotes	stop
T5316	12307-12315	NN	denotes	cassette
T5319	12316-12323	VBN	denotes	flanked
T5320	12324-12326	IN	denotes	by
T5321	12327-12331	NN	denotes	loxP
T5322	12332-12337	NNS	denotes	sites
T5323	12337-12338	.	denotes	.
T5324	12338-12584	sentence	denotes	Targeting cassettes were integrated into the Tau locus to generate two strains of mice conditionally expressing either EWS-Pea3 or a membrane-targeted green fluorescent protein (mGFP) to trace axonal projections of DRG neurons (Figure S3; [25]).
T5325	12339-12348	NN	denotes	Targeting
T5326	12349-12358	NNS	denotes	cassettes
T5328	12359-12363	VBD	denotes	were
T5327	12364-12374	VBN	denotes	integrated
T5329	12375-12379	IN	denotes	into
T5330	12380-12383	DT	denotes	the
T5332	12384-12387	NN	denotes	Tau
T5331	12388-12393	NN	denotes	locus
T5333	12394-12396	TO	denotes	to
T5334	12397-12405	VB	denotes	generate
T5335	12406-12409	CD	denotes	two
T5336	12410-12417	NNS	denotes	strains
T5337	12418-12420	IN	denotes	of
T5338	12421-12425	NNS	denotes	mice
T5339	12426-12439	RB	denotes	conditionally
T5340	12440-12450	VBG	denotes	expressing
T5341	12451-12457	CC	denotes	either
T5343	12458-12461	NN	denotes	EWS
T5344	12461-12462	HYPH	denotes	-
T5342	12462-12466	NN	denotes	Pea3
T5345	12467-12469	CC	denotes	or
T5346	12470-12471	DT	denotes	a
T5348	12472-12480	NN	denotes	membrane
T5350	12480-12481	HYPH	denotes	-
T5349	12481-12489	JJ	denotes	targeted
T5351	12490-12495	JJ	denotes	green
T5352	12496-12507	JJ	denotes	fluorescent
T5347	12508-12515	NN	denotes	protein
T5353	12516-12517	-LRB-	denotes	(
T5354	12517-12521	NN	denotes	mGFP
T5355	12521-12522	-RRB-	denotes	)
T5356	12523-12525	TO	denotes	to
T5357	12526-12531	VB	denotes	trace
T5358	12532-12538	JJ	denotes	axonal
T5359	12539-12550	NNS	denotes	projections
T5360	12551-12553	IN	denotes	of
T5361	12554-12557	NN	denotes	DRG
T5362	12558-12565	NNS	denotes	neurons
T5363	12566-12567	-LRB-	denotes	(
T5365	12567-12573	NN	denotes	Figure
T5366	12574-12576	NN	denotes	S3
T5367	12576-12577	:	denotes	;
T5368	12578-12579	-LRB-	denotes	[
T5364	12579-12581	CD	denotes	25
T5369	12581-12582	-RRB-	denotes	]
T5370	12582-12583	-RRB-	denotes	)
T5371	12583-12584	.	denotes	.
T5372	12584-12836	sentence	denotes	Embryos positive for either Isl1Cre/+ and TauEWS-Pea3/+ or Isl1Cre/+ and TaumGFP/+ alleles showed efficient activation of the silent Tau allele in 95% or more of all DRG neurons, including proprioceptive afferents, at all segmental levels (Figure S3).
T5373	12585-12592	NNS	denotes	Embryos
T5375	12593-12601	JJ	denotes	positive
T5376	12602-12605	IN	denotes	for
T5377	12606-12612	CC	denotes	either
T5378	12613-12620	NN	denotes	Isl1Cre
T5380	12620-12621	HYPH	denotes	/
T5381	12621-12622	SYM	denotes	+
T5382	12623-12626	CC	denotes	and
T5383	12627-12633	NN	denotes	TauEWS
T5385	12633-12634	HYPH	denotes	-
T5384	12634-12638	NN	denotes	Pea3
T5386	12638-12639	HYPH	denotes	/
T5387	12639-12640	SYM	denotes	+
T5388	12641-12643	CC	denotes	or
T5389	12644-12651	NN	denotes	Isl1Cre
T5390	12651-12652	HYPH	denotes	/
T5391	12652-12653	SYM	denotes	+
T5392	12654-12657	CC	denotes	and
T5393	12658-12665	NN	denotes	TaumGFP
T5394	12665-12666	HYPH	denotes	/
T5395	12666-12667	SYM	denotes	+
T5379	12668-12675	NNS	denotes	alleles
T5374	12676-12682	VBD	denotes	showed
T5396	12683-12692	JJ	denotes	efficient
T5397	12693-12703	NN	denotes	activation
T5398	12704-12706	IN	denotes	of
T5399	12707-12710	DT	denotes	the
T5401	12711-12717	JJ	denotes	silent
T5402	12718-12721	NN	denotes	Tau
T5400	12722-12728	NN	denotes	allele
T5403	12729-12731	IN	denotes	in
T5404	12732-12734	CD	denotes	95
T5405	12734-12735	NN	denotes	%
T5406	12736-12738	CC	denotes	or
T5407	12739-12743	JJR	denotes	more
T5408	12744-12746	IN	denotes	of
T5409	12747-12750	DT	denotes	all
T5411	12751-12754	NN	denotes	DRG
T5410	12755-12762	NNS	denotes	neurons
T5412	12762-12764	,	denotes	,
T5413	12764-12773	VBG	denotes	including
T5414	12774-12788	JJ	denotes	proprioceptive
T5415	12789-12798	NNS	denotes	afferents
T5416	12798-12800	,	denotes	,
T5417	12800-12802	IN	denotes	at
T5418	12803-12806	DT	denotes	all
T5420	12807-12816	JJ	denotes	segmental
T5419	12817-12823	NNS	denotes	levels
T5421	12824-12825	-LRB-	denotes	(
T5423	12825-12831	NN	denotes	Figure
T5422	12832-12834	NN	denotes	S3
T5424	12834-12835	-RRB-	denotes	)
T5425	12835-12836	.	denotes	.
T5426	12836-13209	sentence	denotes	We first assessed the influence of EWS-Pea3 expression in early post-mitotic DRG neurons on the establishment of afferent projections into the spinal cord using the TaumGFP/+ allele or a Thy1-promoter-driven synaptophysin green fluorescent protein (spGFP) with an expression profile restricted to DRG sensory neurons at embryonic day (E) 13.5 (Thy1spGFP; [25]) (Figure 3).
T5427	12837-12839	PRP	denotes	We
T5429	12840-12845	RB	denotes	first
T5428	12846-12854	VBD	denotes	assessed
T5430	12855-12858	DT	denotes	the
T5431	12859-12868	NN	denotes	influence
T5432	12869-12871	IN	denotes	of
T5433	12872-12875	NN	denotes	EWS
T5435	12875-12876	HYPH	denotes	-
T5434	12876-12880	NN	denotes	Pea3
T5436	12881-12891	NN	denotes	expression
T5437	12892-12894	IN	denotes	in
T5438	12895-12900	JJ	denotes	early
T5440	12901-12913	JJ	denotes	post-mitotic
T5441	12914-12917	NN	denotes	DRG
T5439	12918-12925	NNS	denotes	neurons
T5442	12926-12928	IN	denotes	on
T5443	12929-12932	DT	denotes	the
T5444	12933-12946	NN	denotes	establishment
T5445	12947-12949	IN	denotes	of
T5446	12950-12958	JJ	denotes	afferent
T5447	12959-12970	NNS	denotes	projections
T5448	12971-12975	IN	denotes	into
T5449	12976-12979	DT	denotes	the
T5451	12980-12986	JJ	denotes	spinal
T5450	12987-12991	NN	denotes	cord
T5452	12992-12997	VBG	denotes	using
T5453	12998-13001	DT	denotes	the
T5455	13002-13009	NN	denotes	TaumGFP
T5456	13009-13010	HYPH	denotes	/
T5457	13010-13011	SYM	denotes	+
T5454	13012-13018	NN	denotes	allele
T5458	13019-13021	CC	denotes	or
T5459	13022-13023	DT	denotes	a
T5461	13024-13028	NN	denotes	Thy1
T5463	13028-13029	HYPH	denotes	-
T5462	13029-13037	NN	denotes	promoter
T5465	13037-13038	HYPH	denotes	-
T5464	13038-13044	VBN	denotes	driven
T5466	13045-13058	NN	denotes	synaptophysin
T5467	13059-13064	JJ	denotes	green
T5468	13065-13076	JJ	denotes	fluorescent
T5460	13077-13084	NN	denotes	protein
T5469	13085-13086	-LRB-	denotes	(
T5470	13086-13091	NN	denotes	spGFP
T5471	13091-13092	-RRB-	denotes	)
T5472	13093-13097	IN	denotes	with
T5474	13098-13100	DT	denotes	an
T5476	13101-13111	NN	denotes	expression
T5475	13112-13119	NN	denotes	profile
T5473	13120-13130	VBN	denotes	restricted
T5477	13131-13133	IN	denotes	to
T5478	13134-13137	NN	denotes	DRG
T5480	13138-13145	JJ	denotes	sensory
T5479	13146-13153	NNS	denotes	neurons
T5481	13154-13156	IN	denotes	at
T5482	13157-13166	JJ	denotes	embryonic
T5483	13167-13170	NN	denotes	day
T5484	13171-13172	-LRB-	denotes	(
T5485	13172-13173	NN	denotes	E
T5486	13173-13174	-RRB-	denotes	)
T5487	13175-13179	CD	denotes	13.5
T5488	13180-13181	-LRB-	denotes	(
T5490	13181-13190	NN	denotes	Thy1spGFP
T5491	13190-13191	:	denotes	;
T5492	13192-13193	-LRB-	denotes	[
T5493	13193-13195	CD	denotes	25
T5494	13195-13196	-RRB-	denotes	]
T5495	13196-13197	-RRB-	denotes	)
T5496	13198-13199	-LRB-	denotes	(
T5489	13199-13205	NN	denotes	Figure
T5497	13206-13207	CD	denotes	3
T5498	13207-13208	-RRB-	denotes	)
T5499	13208-13209	.	denotes	.
T5500	13209-13546	sentence	denotes	In contrast to wild-type proprioceptive afferent projections (Figure 3A–3C), GFP+ sensory afferents in TauEWS-Pea3/+ Isl1Cre/+ embryos failed to invade the spinal cord and instead were found in an extreme lateral position at the dorsal root entry zone, a phenotype observed at least up to E18.5 (Figure 3A–3C and 3G–3I; data not shown).
T5501	13210-13212	IN	denotes	In
T5503	13213-13221	NN	denotes	contrast
T5504	13222-13224	IN	denotes	to
T5505	13225-13229	JJ	denotes	wild
T5507	13229-13230	HYPH	denotes	-
T5506	13230-13234	NN	denotes	type
T5509	13235-13249	JJ	denotes	proprioceptive
T5510	13250-13258	JJ	denotes	afferent
T5508	13259-13270	NNS	denotes	projections
T5511	13271-13272	-LRB-	denotes	(
T5513	13272-13278	NN	denotes	Figure
T5512	13279-13281	NN	denotes	3A
T5514	13281-13282	SYM	denotes	–
T5515	13282-13284	NN	denotes	3C
T5516	13284-13285	-RRB-	denotes	)
T5517	13285-13287	,	denotes	,
T5518	13287-13290	NN	denotes	GFP
T5520	13290-13291	SYM	denotes	+
T5521	13292-13299	JJ	denotes	sensory
T5519	13300-13309	NNS	denotes	afferents
T5522	13310-13312	IN	denotes	in
T5523	13313-13319	NN	denotes	TauEWS
T5525	13319-13320	HYPH	denotes	-
T5524	13320-13324	NN	denotes	Pea3
T5527	13324-13325	HYPH	denotes	/
T5528	13325-13326	SYM	denotes	+
T5529	13327-13334	NN	denotes	Isl1Cre
T5530	13334-13335	HYPH	denotes	/
T5531	13335-13336	SYM	denotes	+
T5526	13337-13344	NNS	denotes	embryos
T5502	13345-13351	VBD	denotes	failed
T5532	13352-13354	TO	denotes	to
T5533	13355-13361	VB	denotes	invade
T5534	13362-13365	DT	denotes	the
T5536	13366-13372	JJ	denotes	spinal
T5535	13373-13377	NN	denotes	cord
T5537	13378-13381	CC	denotes	and
T5538	13382-13389	RB	denotes	instead
T5540	13390-13394	VBD	denotes	were
T5539	13395-13400	VBN	denotes	found
T5541	13401-13403	IN	denotes	in
T5542	13404-13406	DT	denotes	an
T5544	13407-13414	RB	denotes	extreme
T5545	13415-13422	JJ	denotes	lateral
T5543	13423-13431	NN	denotes	position
T5546	13432-13434	IN	denotes	at
T5547	13435-13438	DT	denotes	the
T5549	13439-13445	JJ	denotes	dorsal
T5550	13446-13450	NN	denotes	root
T5551	13451-13456	NN	denotes	entry
T5548	13457-13461	NN	denotes	zone
T5552	13461-13463	,	denotes	,
T5553	13463-13464	DT	denotes	a
T5554	13465-13474	NN	denotes	phenotype
T5555	13475-13483	VBN	denotes	observed
T5556	13484-13486	RB	denotes	at
T5557	13487-13492	RBS	denotes	least
T5558	13493-13495	IN	denotes	up
T5559	13496-13498	IN	denotes	to
T5560	13499-13504	NN	denotes	E18.5
T5561	13505-13506	-LRB-	denotes	(
T5563	13506-13512	NN	denotes	Figure
T5562	13513-13515	NN	denotes	3A
T5564	13515-13516	SYM	denotes	–
T5565	13516-13518	NN	denotes	3C
T5566	13519-13522	CC	denotes	and
T5567	13523-13525	NN	denotes	3G
T5568	13525-13526	SYM	denotes	–
T5569	13526-13528	NN	denotes	3I
T5570	13528-13529	:	denotes	;
T5571	13530-13534	NNS	denotes	data
T5573	13535-13538	RB	denotes	not
T5572	13539-13544	VBN	denotes	shown
T5574	13544-13545	-RRB-	denotes	)
T5575	13545-13546	.	denotes	.
T5576	13546-13769	sentence	denotes	We next visualized the path of sensory afferent projections towards the dorsal root entry zone in TauEWS-Pea3/+ Isl1Cre/+ embryos by injecting fluorescently labeled dextran into an individual DRG (L3; n = 3; Figure 3M–3Q).
T5577	13547-13549	PRP	denotes	We
T5579	13550-13554	RB	denotes	next
T5578	13555-13565	VBD	denotes	visualized
T5580	13566-13569	DT	denotes	the
T5581	13570-13574	NN	denotes	path
T5582	13575-13577	IN	denotes	of
T5583	13578-13585	JJ	denotes	sensory
T5584	13586-13594	JJ	denotes	afferent
T5585	13595-13606	NNS	denotes	projections
T5586	13607-13614	IN	denotes	towards
T5587	13615-13618	DT	denotes	the
T5589	13619-13625	JJ	denotes	dorsal
T5590	13626-13630	NN	denotes	root
T5591	13631-13636	NN	denotes	entry
T5588	13637-13641	NN	denotes	zone
T5592	13642-13644	IN	denotes	in
T5593	13645-13651	NN	denotes	TauEWS
T5595	13651-13652	HYPH	denotes	-
T5596	13652-13656	NN	denotes	Pea3
T5597	13656-13657	HYPH	denotes	/
T5598	13657-13658	SYM	denotes	+
T5594	13659-13666	NN	denotes	Isl1Cre
T5600	13666-13667	HYPH	denotes	/
T5601	13667-13668	SYM	denotes	+
T5599	13669-13676	NNS	denotes	embryos
T5602	13677-13679	IN	denotes	by
T5603	13680-13689	VBG	denotes	injecting
T5604	13690-13703	RB	denotes	fluorescently
T5605	13704-13711	VBN	denotes	labeled
T5606	13712-13719	NN	denotes	dextran
T5607	13720-13724	IN	denotes	into
T5608	13725-13727	DT	denotes	an
T5610	13728-13738	JJ	denotes	individual
T5609	13739-13742	NN	denotes	DRG
T5611	13743-13744	-LRB-	denotes	(
T5613	13744-13746	NN	denotes	L3
T5614	13746-13747	:	denotes	;
T5615	13748-13749	NN	denotes	n
T5617	13750-13751	SYM	denotes	=
T5616	13752-13753	CD	denotes	3
T5618	13753-13754	:	denotes	;
T5619	13755-13761	NN	denotes	Figure
T5612	13762-13764	NN	denotes	3M
T5620	13764-13765	SYM	denotes	–
T5621	13765-13767	NN	denotes	3Q
T5622	13767-13768	-RRB-	denotes	)
T5623	13768-13769	.	denotes	.
T5624	13769-13986	sentence	denotes	Sensory afferents in E13.5 wild-type embryos bifurcated at their lateral spinal entry point, and projected rostrally and caudally over six or more segmental levels while gradually approaching the midline (Figure 3M).
T5625	13770-13777	JJ	denotes	Sensory
T5626	13778-13787	NNS	denotes	afferents
T5628	13788-13790	IN	denotes	in
T5629	13791-13796	NN	denotes	E13.5
T5631	13797-13801	JJ	denotes	wild
T5633	13801-13802	HYPH	denotes	-
T5632	13802-13806	NN	denotes	type
T5630	13807-13814	NNS	denotes	embryos
T5627	13815-13825	VBN	denotes	bifurcated
T5634	13826-13828	IN	denotes	at
T5635	13829-13834	PRP$	denotes	their
T5637	13835-13842	JJ	denotes	lateral
T5638	13843-13849	JJ	denotes	spinal
T5639	13850-13855	NN	denotes	entry
T5636	13856-13861	NN	denotes	point
T5640	13861-13863	,	denotes	,
T5641	13863-13866	CC	denotes	and
T5642	13867-13876	VBN	denotes	projected
T5643	13877-13886	RB	denotes	rostrally
T5644	13887-13890	CC	denotes	and
T5645	13891-13899	RB	denotes	caudally
T5646	13900-13904	IN	denotes	over
T5647	13905-13908	CD	denotes	six
T5649	13909-13911	CC	denotes	or
T5650	13912-13916	JJR	denotes	more
T5651	13917-13926	JJ	denotes	segmental
T5648	13927-13933	NNS	denotes	levels
T5652	13934-13939	IN	denotes	while
T5654	13940-13949	RB	denotes	gradually
T5653	13950-13961	VBG	denotes	approaching
T5655	13962-13965	DT	denotes	the
T5656	13966-13973	NN	denotes	midline
T5657	13974-13975	-LRB-	denotes	(
T5659	13975-13981	NN	denotes	Figure
T5658	13982-13984	NN	denotes	3M
T5660	13984-13985	-RRB-	denotes	)
T5661	13985-13986	.	denotes	.
T5662	13986-14178	sentence	denotes	Sensory afferents in TauEWS-Pea3/+ Isl1Cre/+ embryos also bifurcated at the entry point, although approximately 5% of afferent fibers continued to grow towards the midline (Figure 3O and 3Q).
T5663	13987-13994	JJ	denotes	Sensory
T5664	13995-14004	NNS	denotes	afferents
T5666	14005-14007	IN	denotes	in
T5667	14008-14014	NN	denotes	TauEWS
T5669	14014-14015	HYPH	denotes	-
T5670	14015-14019	NN	denotes	Pea3
T5671	14019-14020	HYPH	denotes	/
T5672	14020-14021	SYM	denotes	+
T5668	14022-14029	NN	denotes	Isl1Cre
T5674	14029-14030	HYPH	denotes	/
T5675	14030-14031	SYM	denotes	+
T5673	14032-14039	NNS	denotes	embryos
T5676	14040-14044	RB	denotes	also
T5665	14045-14055	VBN	denotes	bifurcated
T5677	14056-14058	IN	denotes	at
T5678	14059-14062	DT	denotes	the
T5680	14063-14068	NN	denotes	entry
T5679	14069-14074	NN	denotes	point
T5681	14074-14076	,	denotes	,
T5682	14076-14084	IN	denotes	although
T5684	14085-14098	RB	denotes	approximately
T5685	14099-14100	CD	denotes	5
T5686	14100-14101	NN	denotes	%
T5687	14102-14104	IN	denotes	of
T5688	14105-14113	JJ	denotes	afferent
T5689	14114-14120	NNS	denotes	fibers
T5683	14121-14130	VBD	denotes	continued
T5690	14131-14133	TO	denotes	to
T5691	14134-14138	VB	denotes	grow
T5692	14139-14146	IN	denotes	towards
T5693	14147-14150	DT	denotes	the
T5694	14151-14158	NN	denotes	midline
T5695	14159-14160	-LRB-	denotes	(
T5697	14160-14166	NN	denotes	Figure
T5696	14167-14169	NN	denotes	3O
T5698	14170-14173	CC	denotes	and
T5699	14174-14176	NN	denotes	3Q
T5700	14176-14177	-RRB-	denotes	)
T5701	14177-14178	.	denotes	.
T5702	14178-14443	sentence	denotes	While rostro-caudal projections were present in TauEWS-Pea3/+ Isl1Cre/+ embryos, afferent fibers failed to approach the midline at distal segments and continued to occupy an extreme lateral position (Figure 3O), consistent with the analysis of transverse sections.
T5703	14179-14184	IN	denotes	While
T5705	14185-14191	JJ	denotes	rostro
T5707	14191-14192	HYPH	denotes	-
T5706	14192-14198	JJ	denotes	caudal
T5708	14199-14210	NNS	denotes	projections
T5704	14211-14215	VBD	denotes	were
T5710	14216-14223	JJ	denotes	present
T5711	14224-14226	IN	denotes	in
T5712	14227-14233	NN	denotes	TauEWS
T5714	14233-14234	HYPH	denotes	-
T5715	14234-14238	NN	denotes	Pea3
T5716	14238-14239	HYPH	denotes	/
T5717	14239-14240	SYM	denotes	+
T5713	14241-14248	NN	denotes	Isl1Cre
T5719	14248-14249	HYPH	denotes	/
T5720	14249-14250	SYM	denotes	+
T5718	14251-14258	NNS	denotes	embryos
T5721	14258-14260	,	denotes	,
T5722	14260-14268	JJ	denotes	afferent
T5723	14269-14275	NNS	denotes	fibers
T5709	14276-14282	VBD	denotes	failed
T5724	14283-14285	TO	denotes	to
T5725	14286-14294	VB	denotes	approach
T5726	14295-14298	DT	denotes	the
T5727	14299-14306	NN	denotes	midline
T5728	14307-14309	IN	denotes	at
T5729	14310-14316	JJ	denotes	distal
T5730	14317-14325	NNS	denotes	segments
T5731	14326-14329	CC	denotes	and
T5732	14330-14339	VBD	denotes	continued
T5733	14340-14342	TO	denotes	to
T5734	14343-14349	VB	denotes	occupy
T5735	14350-14352	DT	denotes	an
T5737	14353-14360	RB	denotes	extreme
T5738	14361-14368	JJ	denotes	lateral
T5736	14369-14377	NN	denotes	position
T5739	14378-14379	-LRB-	denotes	(
T5741	14379-14385	NN	denotes	Figure
T5740	14386-14388	NN	denotes	3O
T5742	14388-14389	-RRB-	denotes	)
T5743	14389-14391	,	denotes	,
T5744	14391-14401	JJ	denotes	consistent
T5745	14402-14406	IN	denotes	with
T5746	14407-14410	DT	denotes	the
T5747	14411-14419	NN	denotes	analysis
T5748	14420-14422	IN	denotes	of
T5749	14423-14433	JJ	denotes	transverse
T5750	14434-14442	NNS	denotes	sections
T5751	14442-14443	.	denotes	.
T5752	14443-16239	sentence	denotes	Figure 3 Defects in the Establishment of Sensory Afferent Projections upon Precocious Expression of EWS-Pea3 in DRG Neurons (A–C and G–I) Visualization of sensory afferent projections (green) into the spinal cord of wild-type (A–C) and TauEWS-Pea3/+ Isl1Cre/+ (G–I) embryos at E13.5 (A, C, G, and I) and E16.5 (B and H) by Cre-recombinase-mediated activation of mGFP expression from the Tau locus (A, B, G, and H) or by a Thy1spGFP transgene (C and I; [25]). Grey arrows indicate normal pattern of afferent projections into the spinal cord, whereas red arrows show aberrant accumulation of sensory afferents at the lateral edge of the spinal cord in TauEWS-Pea3/+ Isl1Cre/+ embryos. (D–F and J–L) Analysis of sensory afferent projections (green) into the skin (D and J) or muscle (E and K; red, α-Bungarotoxin, BTX) of wild-type (D–F) and TauEWS-Pea3/+ Isl1Cre/+ (J–L) embryos at E16.5 by Cre-recombinase-mediated activation of mGFP (D, E, J, and K) expression from the Tau locus. (F and L) show Egr3 expression in intrafusal muscle fibers using in situ hybridization (consecutive sections to [E and K] are shown). (M–Q) Analysis of bifurcation of sensory afferent projections towards the spinal cord in E13.5 wild-type (M) and TauEWS-Pea3/+ Isl1Cre/+ (O and Q) embryos after injection of fluorescently labeled dextran (green) into one DRG (lumbar level L3). Confocal scanning plane for (M and O) is schematically illustrated in (N). Inset in (O) is also shown at a deeper confocal scanning plane (P and Q) to visualize aberrant axonal projections. Scale bar: (A and G), 60 μm; (B and H), 80 μm; (C and I), 100 μm; (D and J), 160 μm; (E, F, K, and L), 70 μm; (M, O, and Q), 240 μm. We next examined the establishment of peripheral projections upon precocious EWS-Pea3 expression in DRG neurons.
T5753	16127-16129	PRP	denotes	We
T5755	16130-16134	RB	denotes	next
T5754	16135-16143	VBD	denotes	examined
T5756	16144-16147	DT	denotes	the
T5757	16148-16161	NN	denotes	establishment
T5758	16162-16164	IN	denotes	of
T5759	16165-16175	JJ	denotes	peripheral
T5760	16176-16187	NNS	denotes	projections
T5761	16188-16192	IN	denotes	upon
T5762	16193-16203	JJ	denotes	precocious
T5764	16204-16207	NN	denotes	EWS
T5766	16207-16208	HYPH	denotes	-
T5765	16208-16212	NN	denotes	Pea3
T5763	16213-16223	NN	denotes	expression
T5767	16224-16226	IN	denotes	in
T5768	16227-16230	NN	denotes	DRG
T5769	16231-16238	NNS	denotes	neurons
T5770	16238-16239	.	denotes	.
T5771	16239-16448	sentence	denotes	While sensory axons in TauEWS-Pea3/+ Isl1Cre/+ embryos reached the skin and established major nerve trunks by E16.5, only rudimentary sensory axon branching was established within the skin (Figure 3D and 3J).
T5772	16240-16245	IN	denotes	While
T5774	16246-16253	JJ	denotes	sensory
T5775	16254-16259	NNS	denotes	axons
T5776	16260-16262	IN	denotes	in
T5777	16263-16269	NN	denotes	TauEWS
T5779	16269-16270	HYPH	denotes	-
T5780	16270-16274	NN	denotes	Pea3
T5781	16274-16275	HYPH	denotes	/
T5782	16275-16276	SYM	denotes	+
T5778	16277-16284	NN	denotes	Isl1Cre
T5784	16284-16285	HYPH	denotes	/
T5785	16285-16286	SYM	denotes	+
T5783	16287-16294	NNS	denotes	embryos
T5773	16295-16302	VBD	denotes	reached
T5787	16303-16306	DT	denotes	the
T5788	16307-16311	NN	denotes	skin
T5789	16312-16315	CC	denotes	and
T5790	16316-16327	VBN	denotes	established
T5791	16328-16333	JJ	denotes	major
T5793	16334-16339	NN	denotes	nerve
T5792	16340-16346	NNS	denotes	trunks
T5794	16347-16349	IN	denotes	by
T5795	16350-16355	NN	denotes	E16.5
T5796	16355-16357	,	denotes	,
T5797	16357-16361	RB	denotes	only
T5799	16362-16373	JJ	denotes	rudimentary
T5800	16374-16381	JJ	denotes	sensory
T5801	16382-16386	NN	denotes	axon
T5798	16387-16396	NN	denotes	branching
T5802	16397-16400	VBD	denotes	was
T5786	16401-16412	VBN	denotes	established
T5803	16413-16419	IN	denotes	within
T5804	16420-16423	DT	denotes	the
T5805	16424-16428	NN	denotes	skin
T5806	16429-16430	-LRB-	denotes	(
T5808	16430-16436	NN	denotes	Figure
T5807	16437-16439	NN	denotes	3D
T5809	16440-16443	CC	denotes	and
T5810	16444-16446	NN	denotes	3J
T5811	16446-16447	-RRB-	denotes	)
T5812	16447-16448	.	denotes	.
T5813	16448-16751	sentence	denotes	In addition, there was a significant reduction in the number of muscle spindles in TauEWS-Pea3/+ Isl1Cre/+ embryos (approximately 25% of wild-type complement; n = 3) as assessed by innervation and expression of genes specific for intrafusal muscle fibers such as Egr3 (Figure 3E, 3F, 3K, and 3L; [26]).
T5814	16449-16451	IN	denotes	In
T5816	16452-16460	NN	denotes	addition
T5817	16460-16462	,	denotes	,
T5818	16462-16467	EX	denotes	there
T5815	16468-16471	VBD	denotes	was
T5819	16472-16473	DT	denotes	a
T5821	16474-16485	JJ	denotes	significant
T5820	16486-16495	NN	denotes	reduction
T5822	16496-16498	IN	denotes	in
T5823	16499-16502	DT	denotes	the
T5824	16503-16509	NN	denotes	number
T5825	16510-16512	IN	denotes	of
T5826	16513-16519	NN	denotes	muscle
T5827	16520-16528	NNS	denotes	spindles
T5828	16529-16531	IN	denotes	in
T5829	16532-16538	NN	denotes	TauEWS
T5831	16538-16539	HYPH	denotes	-
T5832	16539-16543	NN	denotes	Pea3
T5833	16543-16544	HYPH	denotes	/
T5834	16544-16545	SYM	denotes	+
T5830	16546-16553	NN	denotes	Isl1Cre
T5836	16553-16554	HYPH	denotes	/
T5837	16554-16555	SYM	denotes	+
T5835	16556-16563	NNS	denotes	embryos
T5838	16564-16565	-LRB-	denotes	(
T5840	16565-16578	RB	denotes	approximately
T5841	16579-16581	CD	denotes	25
T5839	16581-16582	NN	denotes	%
T5842	16583-16585	IN	denotes	of
T5843	16586-16590	JJ	denotes	wild
T5845	16590-16591	HYPH	denotes	-
T5844	16591-16595	NN	denotes	type
T5846	16596-16606	NN	denotes	complement
T5847	16606-16607	:	denotes	;
T5848	16608-16609	NN	denotes	n
T5850	16610-16611	SYM	denotes	=
T5849	16612-16613	CD	denotes	3
T5851	16613-16614	-RRB-	denotes	)
T5852	16615-16617	IN	denotes	as
T5853	16618-16626	VBN	denotes	assessed
T5854	16627-16629	IN	denotes	by
T5855	16630-16641	NN	denotes	innervation
T5856	16642-16645	CC	denotes	and
T5857	16646-16656	NN	denotes	expression
T5858	16657-16659	IN	denotes	of
T5859	16660-16665	NNS	denotes	genes
T5860	16666-16674	JJ	denotes	specific
T5861	16675-16678	IN	denotes	for
T5862	16679-16689	JJ	denotes	intrafusal
T5864	16690-16696	NN	denotes	muscle
T5863	16697-16703	NNS	denotes	fibers
T5865	16704-16708	JJ	denotes	such
T5866	16709-16711	IN	denotes	as
T5867	16712-16716	NN	denotes	Egr3
T5868	16717-16718	-LRB-	denotes	(
T5870	16718-16724	NN	denotes	Figure
T5871	16725-16727	NN	denotes	3E
T5872	16727-16729	,	denotes	,
T5873	16729-16731	NN	denotes	3F
T5874	16731-16733	,	denotes	,
T5875	16733-16735	NN	denotes	3K
T5876	16735-16737	,	denotes	,
T5877	16737-16740	CC	denotes	and
T5878	16741-16743	NN	denotes	3L
T5879	16743-16744	:	denotes	;
T5880	16745-16746	-LRB-	denotes	[
T5869	16746-16748	CD	denotes	26
T5881	16748-16749	-RRB-	denotes	]
T5882	16749-16750	-RRB-	denotes	)
T5883	16750-16751	.	denotes	.
T5884	16751-17071	sentence	denotes	In summary, whereas isochronic expression of EWS-Pea3 promoted the establishment of proprioceptive afferent projections into the ventral spinal cord, precocious expression of the same ETS signaling factor in DRG neurons interfered with establishment of projections into the spinal cord as well as to peripheral targets.
T5885	16752-16754	IN	denotes	In
T5887	16755-16762	NN	denotes	summary
T5888	16762-16764	,	denotes	,
T5889	16764-16771	IN	denotes	whereas
T5891	16772-16782	JJ	denotes	isochronic
T5892	16783-16793	NN	denotes	expression
T5893	16794-16796	IN	denotes	of
T5894	16797-16800	NN	denotes	EWS
T5896	16800-16801	HYPH	denotes	-
T5895	16801-16805	NN	denotes	Pea3
T5890	16806-16814	VBD	denotes	promoted
T5897	16815-16818	DT	denotes	the
T5898	16819-16832	NN	denotes	establishment
T5899	16833-16835	IN	denotes	of
T5900	16836-16850	JJ	denotes	proprioceptive
T5902	16851-16859	JJ	denotes	afferent
T5901	16860-16871	NNS	denotes	projections
T5903	16872-16876	IN	denotes	into
T5904	16877-16880	DT	denotes	the
T5906	16881-16888	JJ	denotes	ventral
T5907	16889-16895	JJ	denotes	spinal
T5905	16896-16900	NN	denotes	cord
T5908	16900-16902	,	denotes	,
T5909	16902-16912	JJ	denotes	precocious
T5910	16913-16923	NN	denotes	expression
T5911	16924-16926	IN	denotes	of
T5912	16927-16930	DT	denotes	the
T5914	16931-16935	JJ	denotes	same
T5915	16936-16939	NN	denotes	ETS
T5916	16940-16949	NN	denotes	signaling
T5913	16950-16956	NN	denotes	factor
T5917	16957-16959	IN	denotes	in
T5918	16960-16963	NN	denotes	DRG
T5919	16964-16971	NNS	denotes	neurons
T5886	16972-16982	VBD	denotes	interfered
T5920	16983-16987	IN	denotes	with
T5921	16988-17001	NN	denotes	establishment
T5922	17002-17004	IN	denotes	of
T5923	17005-17016	NNS	denotes	projections
T5924	17017-17021	IN	denotes	into
T5925	17022-17025	DT	denotes	the
T5927	17026-17032	JJ	denotes	spinal
T5926	17033-17037	NN	denotes	cord
T5928	17038-17040	RB	denotes	as
T5930	17041-17045	RB	denotes	well
T5929	17046-17048	IN	denotes	as
T5931	17049-17051	IN	denotes	to
T5932	17052-17062	JJ	denotes	peripheral
T5933	17063-17070	NNS	denotes	targets
T5934	17070-17071	.	denotes	.
T6663	17073-17083	JJ	denotes	Precocious
T6665	17084-17087	NN	denotes	EWS
T6667	17087-17088	HYPH	denotes	-
T6666	17088-17092	NN	denotes	Pea3
T6664	17093-17103	NN	denotes	Expression
T6668	17104-17112	VBZ	denotes	Promotes
T6669	17113-17125	NN	denotes	Neurotrophin
T6671	17125-17126	HYPH	denotes	-
T6670	17126-17137	JJ	denotes	Independent
T6672	17138-17146	NN	denotes	Survival
T6673	17147-17150	CC	denotes	and
T6674	17151-17158	NN	denotes	Neurite
T6675	17159-17168	NN	denotes	Outgrowth
T6676	17168-17366	sentence	denotes	To begin to address the cellular and molecular mechanisms involved in the distinct biological actions of EWS-Pea3 at different developmental stages, we first turned to in vitro culture experiments.
T6677	17169-17171	TO	denotes	To
T6678	17172-17177	VB	denotes	begin
T6680	17178-17180	TO	denotes	to
T6681	17181-17188	VB	denotes	address
T6682	17189-17192	DT	denotes	the
T6684	17193-17201	JJ	denotes	cellular
T6685	17202-17205	CC	denotes	and
T6686	17206-17215	JJ	denotes	molecular
T6683	17216-17226	NNS	denotes	mechanisms
T6687	17227-17235	VBN	denotes	involved
T6688	17236-17238	IN	denotes	in
T6689	17239-17242	DT	denotes	the
T6691	17243-17251	JJ	denotes	distinct
T6692	17252-17262	JJ	denotes	biological
T6690	17263-17270	NNS	denotes	actions
T6693	17271-17273	IN	denotes	of
T6694	17274-17277	NN	denotes	EWS
T6696	17277-17278	HYPH	denotes	-
T6695	17278-17282	NN	denotes	Pea3
T6697	17283-17285	IN	denotes	at
T6698	17286-17295	JJ	denotes	different
T6700	17296-17309	JJ	denotes	developmental
T6699	17310-17316	NNS	denotes	stages
T6701	17316-17318	,	denotes	,
T6702	17318-17320	PRP	denotes	we
T6703	17321-17326	RB	denotes	first
T6679	17327-17333	VBD	denotes	turned
T6704	17334-17336	IN	denotes	to
T6705	17337-17339	FW	denotes	in
T6706	17340-17345	FW	denotes	vitro
T6708	17346-17353	NN	denotes	culture
T6707	17354-17365	NNS	denotes	experiments
T6709	17365-17366	.	denotes	.
T6710	17366-17633	sentence	denotes	These experiments permit assessment of whether precocious ETS transcription factor signaling influences neuronal survival and in vitro neurite outgrowth of DRG neurons, two parameters prominently influenced by target-derived neurotrophic factors and their receptors.
T6711	17367-17372	DT	denotes	These
T6712	17373-17384	NNS	denotes	experiments
T6713	17385-17391	VBP	denotes	permit
T6714	17392-17402	NN	denotes	assessment
T6715	17403-17405	IN	denotes	of
T6716	17406-17413	IN	denotes	whether
T6718	17414-17424	JJ	denotes	precocious
T6720	17425-17428	NN	denotes	ETS
T6721	17429-17442	NN	denotes	transcription
T6722	17443-17449	NN	denotes	factor
T6719	17450-17459	NN	denotes	signaling
T6717	17460-17470	VBZ	denotes	influences
T6723	17471-17479	JJ	denotes	neuronal
T6724	17480-17488	NN	denotes	survival
T6725	17489-17492	CC	denotes	and
T6726	17493-17495	FW	denotes	in
T6727	17496-17501	FW	denotes	vitro
T6729	17502-17509	NN	denotes	neurite
T6728	17510-17519	NN	denotes	outgrowth
T6730	17520-17522	IN	denotes	of
T6731	17523-17526	NN	denotes	DRG
T6732	17527-17534	NNS	denotes	neurons
T6733	17534-17536	,	denotes	,
T6734	17536-17539	CD	denotes	two
T6735	17540-17550	NNS	denotes	parameters
T6736	17551-17562	RB	denotes	prominently
T6737	17563-17573	VBN	denotes	influenced
T6738	17574-17576	IN	denotes	by
T6739	17577-17583	NN	denotes	target
T6741	17583-17584	HYPH	denotes	-
T6740	17584-17591	VBN	denotes	derived
T6743	17592-17604	JJ	denotes	neurotrophic
T6742	17605-17612	NNS	denotes	factors
T6744	17613-17616	CC	denotes	and
T6745	17617-17622	PRP$	denotes	their
T6746	17623-17632	NNS	denotes	receptors
T6747	17632-17633	.	denotes	.
T6748	17633-17883	sentence	denotes	We cultured E13.5 whole DRG explants from wild-type and TauEWS-Pea3/+ Isl1Cre/+ embryos in the presence of NGF or NT-3 or in the absence of neurotrophins and analyzed neuronal survival and neurite outgrowth on matrigel substrate after 48 h in vitro.
T6749	17634-17636	PRP	denotes	We
T6750	17637-17645	VBD	denotes	cultured
T6751	17646-17651	NN	denotes	E13.5
T6753	17652-17657	JJ	denotes	whole
T6754	17658-17661	NN	denotes	DRG
T6752	17662-17670	NNS	denotes	explants
T6755	17671-17675	IN	denotes	from
T6756	17676-17680	JJ	denotes	wild
T6758	17680-17681	HYPH	denotes	-
T6757	17681-17685	NN	denotes	type
T6760	17686-17689	CC	denotes	and
T6761	17690-17696	NN	denotes	TauEWS
T6763	17696-17697	HYPH	denotes	-
T6764	17697-17701	NN	denotes	Pea3
T6765	17701-17702	HYPH	denotes	/
T6766	17702-17703	SYM	denotes	+
T6762	17704-17711	NN	denotes	Isl1Cre
T6767	17711-17712	HYPH	denotes	/
T6768	17712-17713	SYM	denotes	+
T6759	17714-17721	NNS	denotes	embryos
T6769	17722-17724	IN	denotes	in
T6770	17725-17728	DT	denotes	the
T6771	17729-17737	NN	denotes	presence
T6772	17738-17740	IN	denotes	of
T6773	17741-17744	NN	denotes	NGF
T6774	17745-17747	CC	denotes	or
T6775	17748-17750	NN	denotes	NT
T6776	17750-17751	HYPH	denotes	-
T6777	17751-17752	CD	denotes	3
T6778	17753-17755	CC	denotes	or
T6779	17756-17758	IN	denotes	in
T6780	17759-17762	DT	denotes	the
T6781	17763-17770	NN	denotes	absence
T6782	17771-17773	IN	denotes	of
T6783	17774-17787	NNS	denotes	neurotrophins
T6784	17788-17791	CC	denotes	and
T6785	17792-17800	VBN	denotes	analyzed
T6786	17801-17809	JJ	denotes	neuronal
T6787	17810-17818	NN	denotes	survival
T6788	17819-17822	CC	denotes	and
T6789	17823-17830	NN	denotes	neurite
T6790	17831-17840	NN	denotes	outgrowth
T6791	17841-17843	IN	denotes	on
T6792	17844-17852	NN	denotes	matrigel
T6793	17853-17862	NN	denotes	substrate
T6794	17863-17868	IN	denotes	after
T6795	17869-17871	CD	denotes	48
T6796	17872-17873	NN	denotes	h
T6797	17874-17876	FW	denotes	in
T6798	17877-17882	FW	denotes	vitro
T6799	17882-17883	.	denotes	.
T6800	17883-17966	sentence	denotes	Without neurotrophic support, very few wild-type DRG neurons survived (Figure 4A).
T6801	17884-17891	IN	denotes	Without
T6803	17892-17904	JJ	denotes	neurotrophic
T6804	17905-17912	NN	denotes	support
T6805	17912-17914	,	denotes	,
T6806	17914-17918	RB	denotes	very
T6807	17919-17922	JJ	denotes	few
T6809	17923-17927	JJ	denotes	wild
T6811	17927-17928	HYPH	denotes	-
T6810	17928-17932	NN	denotes	type
T6812	17933-17936	NN	denotes	DRG
T6808	17937-17944	NNS	denotes	neurons
T6802	17945-17953	VBD	denotes	survived
T6813	17954-17955	-LRB-	denotes	(
T6815	17955-17961	NN	denotes	Figure
T6814	17962-17964	NN	denotes	4A
T6816	17964-17965	-RRB-	denotes	)
T6817	17965-17966	.	denotes	.
T6818	17966-18077	sentence	denotes	In contrast, culturing wild-type DRG with neurotrophic factors led to neuronal survival and neurite outgrowth.
T6819	17967-17969	IN	denotes	In
T6821	17970-17978	NN	denotes	contrast
T6822	17978-17980	,	denotes	,
T6823	17980-17989	VBG	denotes	culturing
T6824	17990-17994	JJ	denotes	wild
T6826	17994-17995	HYPH	denotes	-
T6825	17995-17999	NN	denotes	type
T6827	18000-18003	NN	denotes	DRG
T6828	18004-18008	IN	denotes	with
T6829	18009-18021	JJ	denotes	neurotrophic
T6830	18022-18029	NNS	denotes	factors
T6820	18030-18033	VBD	denotes	led
T6831	18034-18036	IN	denotes	to
T6832	18037-18045	JJ	denotes	neuronal
T6833	18046-18054	NN	denotes	survival
T6834	18055-18058	CC	denotes	and
T6835	18059-18066	NN	denotes	neurite
T6836	18067-18076	NN	denotes	outgrowth
T6837	18076-18077	.	denotes	.
T6838	18077-18397	sentence	denotes	Addition of NGF, which supports survival of cutaneous afferents, resulted in straight and unbranched neurite outgrowth (Figure 4B), while cultures grown in the presence of NT-3, which supports survival of proprioceptive afferents, resulted in a highly branched neurite outgrowth pattern after 48 h in vitro (Figure 4C).
T6839	18078-18086	NN	denotes	Addition
T6841	18087-18089	IN	denotes	of
T6842	18090-18093	NN	denotes	NGF
T6843	18093-18095	,	denotes	,
T6844	18095-18100	WDT	denotes	which
T6845	18101-18109	VBZ	denotes	supports
T6846	18110-18118	NN	denotes	survival
T6847	18119-18121	IN	denotes	of
T6848	18122-18131	JJ	denotes	cutaneous
T6849	18132-18141	NNS	denotes	afferents
T6850	18141-18143	,	denotes	,
T6840	18143-18151	VBD	denotes	resulted
T6851	18152-18154	IN	denotes	in
T6852	18155-18163	JJ	denotes	straight
T6854	18164-18167	CC	denotes	and
T6855	18168-18178	JJ	denotes	unbranched
T6856	18179-18186	NN	denotes	neurite
T6853	18187-18196	NN	denotes	outgrowth
T6857	18197-18198	-LRB-	denotes	(
T6859	18198-18204	NN	denotes	Figure
T6858	18205-18207	NN	denotes	4B
T6860	18207-18208	-RRB-	denotes	)
T6861	18208-18210	,	denotes	,
T6862	18210-18215	IN	denotes	while
T6864	18216-18224	NNS	denotes	cultures
T6865	18225-18230	VBN	denotes	grown
T6866	18231-18233	IN	denotes	in
T6867	18234-18237	DT	denotes	the
T6868	18238-18246	NN	denotes	presence
T6869	18247-18249	IN	denotes	of
T6870	18250-18252	NN	denotes	NT
T6871	18252-18253	HYPH	denotes	-
T6872	18253-18254	CD	denotes	3
T6873	18254-18256	,	denotes	,
T6874	18256-18261	WDT	denotes	which
T6875	18262-18270	VBZ	denotes	supports
T6876	18271-18279	NN	denotes	survival
T6877	18280-18282	IN	denotes	of
T6878	18283-18297	JJ	denotes	proprioceptive
T6879	18298-18307	NNS	denotes	afferents
T6880	18307-18309	,	denotes	,
T6863	18309-18317	VBD	denotes	resulted
T6881	18318-18320	IN	denotes	in
T6882	18321-18322	DT	denotes	a
T6884	18323-18329	RB	denotes	highly
T6885	18330-18338	VBN	denotes	branched
T6886	18339-18346	NN	denotes	neurite
T6887	18347-18356	NN	denotes	outgrowth
T6883	18357-18364	NN	denotes	pattern
T6888	18365-18370	IN	denotes	after
T6889	18371-18373	CD	denotes	48
T6890	18374-18375	NN	denotes	h
T6891	18376-18378	FW	denotes	in
T6892	18379-18384	FW	denotes	vitro
T6893	18385-18386	-LRB-	denotes	(
T6895	18386-18392	NN	denotes	Figure
T6894	18393-18395	NN	denotes	4C
T6896	18395-18396	-RRB-	denotes	)
T6897	18396-18397	.	denotes	.
T6898	18397-18607	sentence	denotes	Surprisingly, DRG neurons isolated from TauEWS-Pea3/+ Isl1Cre/+ embryos and cultured without neurotrophic support survived after 48 h in vitro and had established long and highly branched neurites (Figure 4D).
T6899	18398-18410	RB	denotes	Surprisingly
T6901	18410-18412	,	denotes	,
T6902	18412-18415	NN	denotes	DRG
T6903	18416-18423	NNS	denotes	neurons
T6904	18424-18432	VBN	denotes	isolated
T6905	18433-18437	IN	denotes	from
T6906	18438-18444	NN	denotes	TauEWS
T6908	18444-18445	HYPH	denotes	-
T6909	18445-18449	NN	denotes	Pea3
T6910	18449-18450	HYPH	denotes	/
T6911	18450-18451	SYM	denotes	+
T6907	18452-18459	NN	denotes	Isl1Cre
T6913	18459-18460	HYPH	denotes	/
T6914	18460-18461	SYM	denotes	+
T6912	18462-18469	NNS	denotes	embryos
T6915	18470-18473	CC	denotes	and
T6916	18474-18482	VBN	denotes	cultured
T6917	18483-18490	IN	denotes	without
T6918	18491-18503	JJ	denotes	neurotrophic
T6919	18504-18511	NN	denotes	support
T6900	18512-18520	VBD	denotes	survived
T6920	18521-18526	IN	denotes	after
T6921	18527-18529	CD	denotes	48
T6922	18530-18531	NN	denotes	h
T6923	18532-18534	FW	denotes	in
T6924	18535-18540	FW	denotes	vitro
T6925	18541-18544	CC	denotes	and
T6926	18545-18548	VBD	denotes	had
T6927	18549-18560	VBN	denotes	established
T6928	18561-18565	JJ	denotes	long
T6930	18566-18569	CC	denotes	and
T6931	18570-18576	RB	denotes	highly
T6932	18577-18585	VBN	denotes	branched
T6929	18586-18594	NNS	denotes	neurites
T6933	18595-18596	-LRB-	denotes	(
T6935	18596-18602	NN	denotes	Figure
T6934	18603-18605	NN	denotes	4D
T6936	18605-18606	-RRB-	denotes	)
T6937	18606-18607	.	denotes	.
T6938	18607-18752	sentence	denotes	Neither the pattern of neurite outgrowth nor neuronal survival changed significantly after application of either NGF or NT-3 (Figure 4E and 4F).
T6939	18608-18615	CC	denotes	Neither
T6941	18616-18619	DT	denotes	the
T6940	18620-18627	NN	denotes	pattern
T6943	18628-18630	IN	denotes	of
T6944	18631-18638	NN	denotes	neurite
T6945	18639-18648	NN	denotes	outgrowth
T6946	18649-18652	CC	denotes	nor
T6947	18653-18661	JJ	denotes	neuronal
T6948	18662-18670	NN	denotes	survival
T6942	18671-18678	VBD	denotes	changed
T6949	18679-18692	RB	denotes	significantly
T6950	18693-18698	IN	denotes	after
T6951	18699-18710	NN	denotes	application
T6952	18711-18713	IN	denotes	of
T6953	18714-18720	CC	denotes	either
T6954	18721-18724	NN	denotes	NGF
T6955	18725-18727	CC	denotes	or
T6956	18728-18730	NN	denotes	NT
T6957	18730-18731	HYPH	denotes	-
T6958	18731-18732	CD	denotes	3
T6959	18733-18734	-LRB-	denotes	(
T6961	18734-18740	NN	denotes	Figure
T6960	18741-18743	NN	denotes	4E
T6962	18744-18747	CC	denotes	and
T6963	18748-18750	NN	denotes	4F
T6964	18750-18751	-RRB-	denotes	)
T6965	18751-18752	.	denotes	.
T6966	18752-19445	sentence	denotes	Figure 4 Neurotrophin-Independent Neurite Outgrowth In Vitro of DRG Neurons Expressing EWS-Pea3 Precociously E13.5 lumbar DRG from wild-type (A, B, and C), TauEWS-Pea3/+ Isl1Cre/+ (D, E, and F), or Bax−/− (G, H, and I) embryos cultured for 48 h without neurotrophic support (A, D, and G) or in the presence of NGF (B, E, and H) or NT-3 (C, F, and I) were stained for expression of neurofilament to visualize axonal extensions. Scale bar: 130 μm. To directly compare neurotrophin dependence of DRG neurons expressing EWS-Pea3 from the Tau locus at a precocious versus isochronic time of onset, we generated a strain of mice in which Cre recombinase is expressed from the PV locus (Figure S4).
T6967	19200-19202	TO	denotes	To
T6969	19203-19211	RB	denotes	directly
T6968	19212-19219	VB	denotes	compare
T6971	19220-19232	NN	denotes	neurotrophin
T6972	19233-19243	NN	denotes	dependence
T6973	19244-19246	IN	denotes	of
T6974	19247-19250	NN	denotes	DRG
T6975	19251-19258	NNS	denotes	neurons
T6976	19259-19269	VBG	denotes	expressing
T6977	19270-19273	NN	denotes	EWS
T6979	19273-19274	HYPH	denotes	-
T6978	19274-19278	NN	denotes	Pea3
T6980	19279-19283	IN	denotes	from
T6981	19284-19287	DT	denotes	the
T6983	19288-19291	NN	denotes	Tau
T6982	19292-19297	NN	denotes	locus
T6984	19298-19300	IN	denotes	at
T6985	19301-19302	DT	denotes	a
T6987	19303-19313	JJ	denotes	precocious
T6988	19314-19320	CC	denotes	versus
T6989	19321-19331	JJ	denotes	isochronic
T6986	19332-19336	NN	denotes	time
T6990	19337-19339	IN	denotes	of
T6991	19340-19345	NN	denotes	onset
T6992	19345-19347	,	denotes	,
T6993	19347-19349	PRP	denotes	we
T6970	19350-19359	VBD	denotes	generated
T6994	19360-19361	DT	denotes	a
T6995	19362-19368	NN	denotes	strain
T6996	19369-19371	IN	denotes	of
T6997	19372-19376	NNS	denotes	mice
T6998	19377-19379	IN	denotes	in
T7000	19380-19385	WDT	denotes	which
T7001	19386-19389	NN	denotes	Cre
T7002	19390-19401	NN	denotes	recombinase
T7003	19402-19404	VBZ	denotes	is
T6999	19405-19414	VBN	denotes	expressed
T7004	19415-19419	IN	denotes	from
T7005	19420-19423	DT	denotes	the
T7007	19424-19426	NN	denotes	PV
T7006	19427-19432	NN	denotes	locus
T7008	19433-19434	-LRB-	denotes	(
T7010	19434-19440	NN	denotes	Figure
T7009	19441-19443	NN	denotes	S4
T7011	19443-19444	-RRB-	denotes	)
T7012	19444-19445	.	denotes	.
T7013	19445-19630	sentence	denotes	The expression of GFP in TaumGFP/+ PVCre/+ was restricted to PV+ proprioceptive DRG neurons and mirrored the onset of expression of PV at approximately E14 (Figure S4; data not shown).
T7014	19446-19449	DT	denotes	The
T7015	19450-19460	NN	denotes	expression
T7017	19461-19463	IN	denotes	of
T7018	19464-19467	NN	denotes	GFP
T7019	19468-19470	IN	denotes	in
T7020	19471-19478	NN	denotes	TaumGFP
T7022	19478-19479	HYPH	denotes	/
T7023	19479-19480	SYM	denotes	+
T7021	19481-19486	NN	denotes	PVCre
T7024	19486-19487	HYPH	denotes	/
T7025	19487-19488	SYM	denotes	+
T7026	19489-19492	VBD	denotes	was
T7016	19493-19503	VBN	denotes	restricted
T7027	19504-19506	IN	denotes	to
T7028	19507-19509	NN	denotes	PV
T7030	19509-19510	SYM	denotes	+
T7031	19511-19525	JJ	denotes	proprioceptive
T7032	19526-19529	NN	denotes	DRG
T7029	19530-19537	NNS	denotes	neurons
T7033	19538-19541	CC	denotes	and
T7034	19542-19550	VBD	denotes	mirrored
T7035	19551-19554	DT	denotes	the
T7036	19555-19560	NN	denotes	onset
T7037	19561-19563	IN	denotes	of
T7038	19564-19574	NN	denotes	expression
T7039	19575-19577	IN	denotes	of
T7040	19578-19580	NN	denotes	PV
T7041	19581-19583	IN	denotes	at
T7042	19584-19597	RB	denotes	approximately
T7043	19598-19601	NN	denotes	E14
T7044	19602-19603	-LRB-	denotes	(
T7046	19603-19609	NN	denotes	Figure
T7045	19610-19612	NN	denotes	S4
T7047	19612-19613	:	denotes	;
T7048	19614-19618	NNS	denotes	data
T7050	19619-19622	RB	denotes	not
T7049	19623-19628	VBN	denotes	shown
T7051	19628-19629	-RRB-	denotes	)
T7052	19629-19630	.	denotes	.
T7053	19630-19791	sentence	denotes	We next cultured E14.5 whole DRG explants from TauEWS-Pea3/+ PVCre/+ and TaumGFP/+ PVCre/+ mice for 48 h in vitro in the presence or absence of NT-3 (Figure 5).
T7054	19631-19633	PRP	denotes	We
T7056	19634-19638	RB	denotes	next
T7055	19639-19647	VBN	denotes	cultured
T7057	19648-19653	NN	denotes	E14.5
T7059	19654-19659	JJ	denotes	whole
T7060	19660-19663	NN	denotes	DRG
T7058	19664-19672	NNS	denotes	explants
T7061	19673-19677	IN	denotes	from
T7062	19678-19684	NN	denotes	TauEWS
T7064	19684-19685	HYPH	denotes	-
T7065	19685-19689	NN	denotes	Pea3
T7066	19689-19690	HYPH	denotes	/
T7067	19690-19691	SYM	denotes	+
T7063	19692-19697	NN	denotes	PVCre
T7069	19697-19698	HYPH	denotes	/
T7070	19698-19699	SYM	denotes	+
T7071	19700-19703	CC	denotes	and
T7072	19704-19711	NN	denotes	TaumGFP
T7074	19711-19712	HYPH	denotes	/
T7075	19712-19713	SYM	denotes	+
T7073	19714-19719	NN	denotes	PVCre
T7076	19719-19720	HYPH	denotes	/
T7077	19720-19721	SYM	denotes	+
T7068	19722-19726	NNS	denotes	mice
T7078	19727-19730	IN	denotes	for
T7079	19731-19733	CD	denotes	48
T7080	19734-19735	NN	denotes	h
T7081	19736-19738	FW	denotes	in
T7082	19739-19744	FW	denotes	vitro
T7083	19745-19747	IN	denotes	in
T7084	19748-19751	DT	denotes	the
T7085	19752-19760	NN	denotes	presence
T7086	19761-19763	CC	denotes	or
T7087	19764-19771	NN	denotes	absence
T7088	19772-19774	IN	denotes	of
T7089	19775-19777	NN	denotes	NT
T7090	19777-19778	HYPH	denotes	-
T7091	19778-19779	CD	denotes	3
T7092	19780-19781	-LRB-	denotes	(
T7093	19781-19787	NN	denotes	Figure
T7094	19788-19789	CD	denotes	5
T7095	19789-19790	-RRB-	denotes	)
T7096	19790-19791	.	denotes	.
T7097	19791-19951	sentence	denotes	We found that DRG neurons from both genotypes survived and extended neurites only in the presence of NT-3, whereas they died in the absence of NT-3 (Figure 5).
T7098	19792-19794	PRP	denotes	We
T7099	19795-19800	VBD	denotes	found
T7100	19801-19805	IN	denotes	that
T7102	19806-19809	NN	denotes	DRG
T7103	19810-19817	NNS	denotes	neurons
T7104	19818-19822	IN	denotes	from
T7105	19823-19827	DT	denotes	both
T7106	19828-19837	NNS	denotes	genotypes
T7101	19838-19846	VBD	denotes	survived
T7107	19847-19850	CC	denotes	and
T7108	19851-19859	VBD	denotes	extended
T7109	19860-19868	NNS	denotes	neurites
T7110	19869-19873	RB	denotes	only
T7111	19874-19876	IN	denotes	in
T7112	19877-19880	DT	denotes	the
T7113	19881-19889	NN	denotes	presence
T7114	19890-19892	IN	denotes	of
T7115	19893-19895	NN	denotes	NT
T7116	19895-19896	HYPH	denotes	-
T7117	19896-19897	CD	denotes	3
T7118	19897-19899	,	denotes	,
T7119	19899-19906	IN	denotes	whereas
T7121	19907-19911	PRP	denotes	they
T7120	19912-19916	VBD	denotes	died
T7122	19917-19919	IN	denotes	in
T7123	19920-19923	DT	denotes	the
T7124	19924-19931	NN	denotes	absence
T7125	19932-19934	IN	denotes	of
T7126	19935-19937	NN	denotes	NT
T7127	19937-19938	HYPH	denotes	-
T7128	19938-19939	CD	denotes	3
T7129	19940-19941	-LRB-	denotes	(
T7130	19941-19947	NN	denotes	Figure
T7131	19948-19949	CD	denotes	5
T7132	19949-19950	-RRB-	denotes	)
T7133	19950-19951	.	denotes	.
T7134	19951-20191	sentence	denotes	Together, these findings suggest that only precocious but not isochronic ETS signaling in DRG neurons is capable of uncoupling survival and neurite outgrowth from a requirement for neurotrophin signaling normally observed in wild-type DRG.
T7135	19952-19960	RB	denotes	Together
T7137	19960-19962	,	denotes	,
T7138	19962-19967	DT	denotes	these
T7139	19968-19976	NNS	denotes	findings
T7136	19977-19984	VBP	denotes	suggest
T7140	19985-19989	IN	denotes	that
T7142	19990-19994	RB	denotes	only
T7144	19995-20005	JJ	denotes	precocious
T7145	20006-20009	CC	denotes	but
T7146	20010-20013	RB	denotes	not
T7147	20014-20024	JJ	denotes	isochronic
T7148	20025-20028	NN	denotes	ETS
T7143	20029-20038	NN	denotes	signaling
T7149	20039-20041	IN	denotes	in
T7150	20042-20045	NN	denotes	DRG
T7151	20046-20053	NNS	denotes	neurons
T7141	20054-20056	VBZ	denotes	is
T7152	20057-20064	JJ	denotes	capable
T7153	20065-20067	IN	denotes	of
T7154	20068-20078	VBG	denotes	uncoupling
T7155	20079-20087	NN	denotes	survival
T7156	20088-20091	CC	denotes	and
T7157	20092-20099	NN	denotes	neurite
T7158	20100-20109	NN	denotes	outgrowth
T7159	20110-20114	IN	denotes	from
T7160	20115-20116	DT	denotes	a
T7161	20117-20128	NN	denotes	requirement
T7162	20129-20132	IN	denotes	for
T7163	20133-20145	NN	denotes	neurotrophin
T7164	20146-20155	NN	denotes	signaling
T7165	20156-20164	RB	denotes	normally
T7166	20165-20173	VBN	denotes	observed
T7167	20174-20176	IN	denotes	in
T7168	20177-20181	JJ	denotes	wild
T7170	20181-20182	HYPH	denotes	-
T7169	20182-20186	NN	denotes	type
T7171	20187-20190	NN	denotes	DRG
T7172	20190-20191	.	denotes	.
T7173	20191-20912	sentence	denotes	Figure 5 DRG Neurons Expressing EWS-Pea3 Isochronically Depend on Neurotrophins for Survival E14.5 lumbar DRG from TaumGFP/+ PVCre/+ (A and B) and TauEWS-Pea3/+ PVCre/+ (C and D) embryos cultured for 48 h without neurotrophic support (A and C) or in the presence of NT-3 (B and D) were stained for expression of neurofilament (red) and LacZ (green) to visualize axonal extensions and survival of PV-expressing proprioceptive afferents. Scale bar: 150 μm. To determine whether neuronal survival of DRG neurons from TauEWS-Pea3/+ Isl1Cre/+ embryos in the absence of neurotrophic support is sufficient to explain the observed neuronal outgrowth, we analyzed DRG isolated from mice mutant in the proapoptotic gene Bax [27].
T7174	20648-20650	TO	denotes	To
T7175	20651-20660	VB	denotes	determine
T7177	20661-20668	IN	denotes	whether
T7179	20669-20677	JJ	denotes	neuronal
T7180	20678-20686	NN	denotes	survival
T7181	20687-20689	IN	denotes	of
T7182	20690-20693	NN	denotes	DRG
T7183	20694-20701	NNS	denotes	neurons
T7184	20702-20706	IN	denotes	from
T7185	20707-20713	NN	denotes	TauEWS
T7187	20713-20714	HYPH	denotes	-
T7188	20714-20718	NN	denotes	Pea3
T7189	20718-20719	HYPH	denotes	/
T7190	20719-20720	SYM	denotes	+
T7186	20721-20728	NN	denotes	Isl1Cre
T7192	20728-20729	HYPH	denotes	/
T7193	20729-20730	SYM	denotes	+
T7191	20731-20738	NNS	denotes	embryos
T7194	20739-20741	IN	denotes	in
T7195	20742-20745	DT	denotes	the
T7196	20746-20753	NN	denotes	absence
T7197	20754-20756	IN	denotes	of
T7198	20757-20769	JJ	denotes	neurotrophic
T7199	20770-20777	NN	denotes	support
T7178	20778-20780	VBZ	denotes	is
T7200	20781-20791	JJ	denotes	sufficient
T7201	20792-20794	TO	denotes	to
T7202	20795-20802	VB	denotes	explain
T7203	20803-20806	DT	denotes	the
T7205	20807-20815	VBN	denotes	observed
T7206	20816-20824	JJ	denotes	neuronal
T7204	20825-20834	NN	denotes	outgrowth
T7207	20834-20836	,	denotes	,
T7208	20836-20838	PRP	denotes	we
T7176	20839-20847	VBD	denotes	analyzed
T7209	20848-20851	NN	denotes	DRG
T7210	20852-20860	VBN	denotes	isolated
T7211	20861-20865	IN	denotes	from
T7212	20866-20870	NNS	denotes	mice
T7213	20871-20877	NN	denotes	mutant
T7214	20878-20880	IN	denotes	in
T7215	20881-20884	DT	denotes	the
T7217	20885-20897	JJ	denotes	proapoptotic
T7218	20898-20902	NN	denotes	gene
T7216	20903-20906	NN	denotes	Bax
T7219	20907-20908	-LRB-	denotes	[
T7220	20908-20910	CD	denotes	27
T7221	20910-20911	-RRB-	denotes	]
T7222	20911-20912	.	denotes	.
T7223	20912-21009	sentence	denotes	Consistent with previous results, Bax−/− DRG neurons survived without neurotrophic support [28].
T7224	20913-20923	JJ	denotes	Consistent
T7226	20924-20928	IN	denotes	with
T7227	20929-20937	JJ	denotes	previous
T7228	20938-20945	NNS	denotes	results
T7229	20945-20947	,	denotes	,
T7230	20947-20950	NN	denotes	Bax
T7232	20950-20951	SYM	denotes	−
T7233	20951-20952	HYPH	denotes	/
T7234	20952-20953	SYM	denotes	−
T7235	20954-20957	NN	denotes	DRG
T7231	20958-20965	NNS	denotes	neurons
T7225	20966-20974	VBD	denotes	survived
T7236	20975-20982	IN	denotes	without
T7237	20983-20995	JJ	denotes	neurotrophic
T7238	20996-21003	NN	denotes	support
T7239	21004-21005	-LRB-	denotes	[
T7240	21005-21007	CD	denotes	28
T7241	21007-21008	-RRB-	denotes	]
T7242	21008-21009	.	denotes	.
T7243	21009-21321	sentence	denotes	In contrast, neurite outgrowth of Bax−/− DRG neurons was significantly less (see Figure 4G) than that of either DRG from TauEWS-Pea3/+ Isl1Cre/+ embryos cultured in the absence of neurotrophic support (see Figure 4D) or Bax−/− DRG neurons cultured in the presence of neurotrophic support (see Figure 4H and 4I).
T7244	21010-21012	IN	denotes	In
T7246	21013-21021	NN	denotes	contrast
T7247	21021-21023	,	denotes	,
T7248	21023-21030	NN	denotes	neurite
T7249	21031-21040	NN	denotes	outgrowth
T7250	21041-21043	IN	denotes	of
T7251	21044-21047	NN	denotes	Bax
T7253	21047-21048	SYM	denotes	−
T7254	21048-21049	HYPH	denotes	/
T7255	21049-21050	SYM	denotes	−
T7256	21051-21054	NN	denotes	DRG
T7252	21055-21062	NNS	denotes	neurons
T7245	21063-21066	VBD	denotes	was
T7257	21067-21080	RB	denotes	significantly
T7258	21081-21085	JJR	denotes	less
T7259	21086-21087	-LRB-	denotes	(
T7260	21087-21090	VB	denotes	see
T7261	21091-21097	NN	denotes	Figure
T7262	21098-21100	NN	denotes	4G
T7263	21100-21101	-RRB-	denotes	)
T7264	21102-21106	IN	denotes	than
T7265	21107-21111	DT	denotes	that
T7266	21112-21114	IN	denotes	of
T7267	21115-21121	CC	denotes	either
T7268	21122-21125	NN	denotes	DRG
T7269	21126-21130	IN	denotes	from
T7270	21131-21137	NN	denotes	TauEWS
T7272	21137-21138	HYPH	denotes	-
T7273	21138-21142	NN	denotes	Pea3
T7274	21142-21143	HYPH	denotes	/
T7275	21143-21144	SYM	denotes	+
T7271	21145-21152	NN	denotes	Isl1Cre
T7277	21152-21153	HYPH	denotes	/
T7278	21153-21154	SYM	denotes	+
T7276	21155-21162	NNS	denotes	embryos
T7279	21163-21171	VBN	denotes	cultured
T7280	21172-21174	IN	denotes	in
T7281	21175-21178	DT	denotes	the
T7282	21179-21186	NN	denotes	absence
T7283	21187-21189	IN	denotes	of
T7284	21190-21202	JJ	denotes	neurotrophic
T7285	21203-21210	NN	denotes	support
T7286	21211-21212	-LRB-	denotes	(
T7287	21212-21215	VB	denotes	see
T7288	21216-21222	NN	denotes	Figure
T7289	21223-21225	NN	denotes	4D
T7290	21225-21226	-RRB-	denotes	)
T7291	21227-21229	CC	denotes	or
T7292	21230-21233	NN	denotes	Bax
T7294	21233-21234	SYM	denotes	−
T7295	21234-21235	HYPH	denotes	/
T7296	21235-21236	SYM	denotes	−
T7297	21237-21240	NN	denotes	DRG
T7293	21241-21248	NNS	denotes	neurons
T7298	21249-21257	VBN	denotes	cultured
T7299	21258-21260	IN	denotes	in
T7300	21261-21264	DT	denotes	the
T7301	21265-21273	NN	denotes	presence
T7302	21274-21276	IN	denotes	of
T7303	21277-21289	JJ	denotes	neurotrophic
T7304	21290-21297	NN	denotes	support
T7305	21298-21299	-LRB-	denotes	(
T7306	21299-21302	VB	denotes	see
T7307	21303-21309	NN	denotes	Figure
T7308	21310-21312	NN	denotes	4H
T7309	21313-21316	CC	denotes	and
T7310	21317-21319	NN	denotes	4I
T7311	21319-21320	-RRB-	denotes	)
T7312	21320-21321	.	denotes	.
T7313	21321-21541	sentence	denotes	These findings suggest that in addition to mediating neurotrophin-independent neuronal survival, expression of EWS-Pea3 in early post-mitotic neurons also promotes neurite outgrowth in a neurotrophin-independent manner.
T7314	21322-21327	DT	denotes	These
T7315	21328-21336	NNS	denotes	findings
T7316	21337-21344	VBP	denotes	suggest
T7317	21345-21349	IN	denotes	that
T7319	21350-21352	IN	denotes	in
T7320	21353-21361	NN	denotes	addition
T7321	21362-21364	IN	denotes	to
T7322	21365-21374	VBG	denotes	mediating
T7323	21375-21387	NN	denotes	neurotrophin
T7325	21387-21388	HYPH	denotes	-
T7324	21388-21399	JJ	denotes	independent
T7327	21400-21408	JJ	denotes	neuronal
T7326	21409-21417	NN	denotes	survival
T7328	21417-21419	,	denotes	,
T7329	21419-21429	NN	denotes	expression
T7330	21430-21432	IN	denotes	of
T7331	21433-21436	NN	denotes	EWS
T7333	21436-21437	HYPH	denotes	-
T7332	21437-21441	NN	denotes	Pea3
T7334	21442-21444	IN	denotes	in
T7335	21445-21450	JJ	denotes	early
T7337	21451-21463	JJ	denotes	post-mitotic
T7336	21464-21471	NNS	denotes	neurons
T7338	21472-21476	RB	denotes	also
T7318	21477-21485	VBZ	denotes	promotes
T7339	21486-21493	NN	denotes	neurite
T7340	21494-21503	NN	denotes	outgrowth
T7341	21504-21506	IN	denotes	in
T7342	21507-21508	DT	denotes	a
T7344	21509-21521	NN	denotes	neurotrophin
T7346	21521-21522	HYPH	denotes	-
T7345	21522-21533	JJ	denotes	independent
T7343	21534-21540	NN	denotes	manner
T7347	21540-21541	.	denotes	.
T7348	21541-21814	sentence	denotes	To begin to assess at which step of the neurotrophin signaling cascade DRG neurons from TauEWS-Pea3/+ Isl1Cre/+ embryos become unresponsive to the addition of neurotrophins, we assayed the expression of neurotrophin receptors in TauEWS-Pea3/+ Isl1Cre/+ embryos (Figure 6).
T7349	21542-21544	TO	denotes	To
T7350	21545-21550	VB	denotes	begin
T7352	21551-21553	TO	denotes	to
T7353	21554-21560	VB	denotes	assess
T7354	21561-21563	IN	denotes	at
T7356	21564-21569	WDT	denotes	which
T7357	21570-21574	NN	denotes	step
T7358	21575-21577	IN	denotes	of
T7359	21578-21581	DT	denotes	the
T7361	21582-21594	NN	denotes	neurotrophin
T7362	21595-21604	NN	denotes	signaling
T7360	21605-21612	NN	denotes	cascade
T7363	21613-21616	NN	denotes	DRG
T7364	21617-21624	NNS	denotes	neurons
T7365	21625-21629	IN	denotes	from
T7366	21630-21636	NN	denotes	TauEWS
T7368	21636-21637	HYPH	denotes	-
T7369	21637-21641	NN	denotes	Pea3
T7370	21641-21642	HYPH	denotes	/
T7371	21642-21643	SYM	denotes	+
T7367	21644-21651	NN	denotes	Isl1Cre
T7373	21651-21652	HYPH	denotes	/
T7374	21652-21653	SYM	denotes	+
T7372	21654-21661	NNS	denotes	embryos
T7355	21662-21668	VBP	denotes	become
T7375	21669-21681	JJ	denotes	unresponsive
T7376	21682-21684	IN	denotes	to
T7377	21685-21688	DT	denotes	the
T7378	21689-21697	NN	denotes	addition
T7379	21698-21700	IN	denotes	of
T7380	21701-21714	NNS	denotes	neurotrophins
T7381	21714-21716	,	denotes	,
T7382	21716-21718	PRP	denotes	we
T7351	21719-21726	VBD	denotes	assayed
T7383	21727-21730	DT	denotes	the
T7384	21731-21741	NN	denotes	expression
T7385	21742-21744	IN	denotes	of
T7386	21745-21757	NN	denotes	neurotrophin
T7387	21758-21767	NNS	denotes	receptors
T7388	21768-21770	IN	denotes	in
T7389	21771-21777	NN	denotes	TauEWS
T7391	21777-21778	HYPH	denotes	-
T7392	21778-21782	NN	denotes	Pea3
T7393	21782-21783	HYPH	denotes	/
T7394	21783-21784	SYM	denotes	+
T7390	21785-21792	NN	denotes	Isl1Cre
T7396	21792-21793	HYPH	denotes	/
T7397	21793-21794	SYM	denotes	+
T7395	21795-21802	NNS	denotes	embryos
T7398	21803-21804	-LRB-	denotes	(
T7399	21804-21810	NN	denotes	Figure
T7400	21811-21812	CD	denotes	6
T7401	21812-21813	-RRB-	denotes	)
T7402	21813-21814	.	denotes	.
T7403	21814-22116	sentence	denotes	Whereas expression of the neurotrophin receptors TrkA, TrkB, and TrkC marks afferents of distinct sensory modalities in DRG of wild-type embryos (Figure 6A–6C) [4,29], TauEWS-Pea3/+ Isl1Cre/+ embryos showed complete absence of expression of TrkA, TrkB, and TrkC in DRG neurons at E16.5 (Figure 6G–6I).
T7404	21815-21822	IN	denotes	Whereas
T7406	21823-21833	NN	denotes	expression
T7407	21834-21836	IN	denotes	of
T7408	21837-21840	DT	denotes	the
T7410	21841-21853	NN	denotes	neurotrophin
T7409	21854-21863	NNS	denotes	receptors
T7411	21864-21868	NN	denotes	TrkA
T7412	21868-21870	,	denotes	,
T7413	21870-21874	NN	denotes	TrkB
T7414	21874-21876	,	denotes	,
T7415	21876-21879	CC	denotes	and
T7416	21880-21884	NN	denotes	TrkC
T7405	21885-21890	VBZ	denotes	marks
T7418	21891-21900	NNS	denotes	afferents
T7419	21901-21903	IN	denotes	of
T7420	21904-21912	JJ	denotes	distinct
T7422	21913-21920	JJ	denotes	sensory
T7421	21921-21931	NNS	denotes	modalities
T7423	21932-21934	IN	denotes	in
T7424	21935-21938	NN	denotes	DRG
T7425	21939-21941	IN	denotes	of
T7426	21942-21946	JJ	denotes	wild
T7428	21946-21947	HYPH	denotes	-
T7427	21947-21951	NN	denotes	type
T7429	21952-21959	NNS	denotes	embryos
T7430	21960-21961	-LRB-	denotes	(
T7432	21961-21967	NN	denotes	Figure
T7431	21968-21970	NN	denotes	6A
T7433	21970-21971	SYM	denotes	–
T7434	21971-21973	NN	denotes	6C
T7435	21973-21974	-RRB-	denotes	)
T7436	21975-21976	-LRB-	denotes	[
T7438	21976-21977	CD	denotes	4
T7439	21977-21978	,	denotes	,
T7437	21978-21980	CD	denotes	29
T7440	21980-21981	-RRB-	denotes	]
T7441	21981-21983	,	denotes	,
T7442	21983-21989	NN	denotes	TauEWS
T7444	21989-21990	HYPH	denotes	-
T7445	21990-21994	NN	denotes	Pea3
T7446	21994-21995	HYPH	denotes	/
T7447	21995-21996	SYM	denotes	+
T7443	21997-22004	NN	denotes	Isl1Cre
T7449	22004-22005	HYPH	denotes	/
T7450	22005-22006	SYM	denotes	+
T7448	22007-22014	NNS	denotes	embryos
T7417	22015-22021	VBD	denotes	showed
T7451	22022-22030	JJ	denotes	complete
T7452	22031-22038	NN	denotes	absence
T7453	22039-22041	IN	denotes	of
T7454	22042-22052	NN	denotes	expression
T7455	22053-22055	IN	denotes	of
T7456	22056-22060	NN	denotes	TrkA
T7457	22060-22062	,	denotes	,
T7458	22062-22066	NN	denotes	TrkB
T7459	22066-22068	,	denotes	,
T7460	22068-22071	CC	denotes	and
T7461	22072-22076	NN	denotes	TrkC
T7462	22077-22079	IN	denotes	in
T7463	22080-22083	NN	denotes	DRG
T7464	22084-22091	NNS	denotes	neurons
T7465	22092-22094	IN	denotes	at
T7466	22095-22100	NN	denotes	E16.5
T7467	22101-22102	-LRB-	denotes	(
T7469	22102-22108	NN	denotes	Figure
T7468	22109-22111	NN	denotes	6G
T7470	22111-22112	SYM	denotes	–
T7471	22112-22114	NN	denotes	6I
T7472	22114-22115	-RRB-	denotes	)
T7473	22115-22116	.	denotes	.
T7474	22116-22317	sentence	denotes	This absence of Trk receptor expression in DRG of TauEWS-Pea3/+ Isl1Cre/+ embryos provides a likely explanation for the lack of responsiveness of these neurons to the addition of neurotrophic factors.
T7475	22117-22121	DT	denotes	This
T7476	22122-22129	NN	denotes	absence
T7478	22130-22132	IN	denotes	of
T7479	22133-22136	NN	denotes	Trk
T7481	22137-22145	NN	denotes	receptor
T7480	22146-22156	NN	denotes	expression
T7482	22157-22159	IN	denotes	in
T7483	22160-22163	NN	denotes	DRG
T7484	22164-22166	IN	denotes	of
T7485	22167-22173	NN	denotes	TauEWS
T7487	22173-22174	HYPH	denotes	-
T7488	22174-22178	NN	denotes	Pea3
T7489	22178-22179	HYPH	denotes	/
T7490	22179-22180	SYM	denotes	+
T7486	22181-22188	NN	denotes	Isl1Cre
T7492	22188-22189	HYPH	denotes	/
T7493	22189-22190	SYM	denotes	+
T7491	22191-22198	NNS	denotes	embryos
T7477	22199-22207	VBZ	denotes	provides
T7494	22208-22209	DT	denotes	a
T7496	22210-22216	JJ	denotes	likely
T7495	22217-22228	NN	denotes	explanation
T7497	22229-22232	IN	denotes	for
T7498	22233-22236	DT	denotes	the
T7499	22237-22241	NN	denotes	lack
T7500	22242-22244	IN	denotes	of
T7501	22245-22259	NN	denotes	responsiveness
T7502	22260-22262	IN	denotes	of
T7503	22263-22268	DT	denotes	these
T7504	22269-22276	NNS	denotes	neurons
T7505	22277-22279	IN	denotes	to
T7506	22280-22283	DT	denotes	the
T7507	22284-22292	NN	denotes	addition
T7508	22293-22295	IN	denotes	of
T7509	22296-22308	JJ	denotes	neurotrophic
T7510	22309-22316	NNS	denotes	factors
T7511	22316-22317	.	denotes	.
T7512	22317-23922	sentence	denotes	Figure 6 Loss of Trk Receptor Expression and Increased Survival in DRG Neurons upon Precocious ETS Signaling (A–C and G–I) In situ hybridization analysis of TrkA (A and G), TrkB (B and H), and TrkC (C and I) expression in E16.5 lumbar DRG of wild-type (A–C) and TauEWS-Pea3/+ Isl1Cre/+ (G–I) embryos. (D–F and J–L) Analysis of lumbar DRG of wild-type (D), TaumGFP/+ IslCre/+ (E and F), and TauEWS-Pea3/+ Isl1Cre/+ (J, K, and L) embryos for (1) neuronal cell death at E13.5 by TUNEL (green; D and J), (2) cell survival and proliferation at E16.5 by LacZ (blue) wholemount staining (E and K; lumbar levels L1 and L2 are shown), and (3) BrdU (green)/LacZ (red) double labeling (F and L). (M and N) Quantitative analysis (n ≥ 3 independent experiments) of the mean number of apoptotic events relative to wild-type levels is shown in (M) and neuronal survival in (N) as percent of wild-type of DRG at lumbar levels L1 to L5 as quantified on serial sections. (O) Western blot analysis of protein extracts isolated from lumbar DRG of E16.5 wild-type (wt) and TauEWS-Pea3/+ Isl1Cre/+ (mut) embryos using the following antibodies: Akt, p-Akt (Ser473), CREB, p-CREB (Ser133), Bax, Bcl2, and Bcl-xl. (P) Quantitative analysis of protein levels relative to wild-type in percent is shown on the right (n = 3 independent experiments). Scale bar: (A–C and G–I), 35 μm; (D and J), 40 μm; (E and K), 200 μm; (F and L), 50 μm. We next assayed whether the complete absence of Trk receptor expression in TauEWS-Pea3/+ Isl1Cre/+ embryos had an influence on naturally occurring cell death in vivo using TUNEL on DRG sections.
T7513	23728-23730	PRP	denotes	We
T7515	23731-23735	RB	denotes	next
T7514	23736-23743	VBD	denotes	assayed
T7516	23744-23751	IN	denotes	whether
T7518	23752-23755	DT	denotes	the
T7520	23756-23764	JJ	denotes	complete
T7519	23765-23772	NN	denotes	absence
T7521	23773-23775	IN	denotes	of
T7522	23776-23779	NN	denotes	Trk
T7524	23780-23788	NN	denotes	receptor
T7523	23789-23799	NN	denotes	expression
T7525	23800-23802	IN	denotes	in
T7526	23803-23809	NN	denotes	TauEWS
T7528	23809-23810	HYPH	denotes	-
T7529	23810-23814	NN	denotes	Pea3
T7530	23814-23815	HYPH	denotes	/
T7531	23815-23816	SYM	denotes	+
T7527	23817-23824	NN	denotes	Isl1Cre
T7533	23824-23825	HYPH	denotes	/
T7534	23825-23826	SYM	denotes	+
T7532	23827-23834	NNS	denotes	embryos
T7517	23835-23838	VBD	denotes	had
T7535	23839-23841	DT	denotes	an
T7536	23842-23851	NN	denotes	influence
T7537	23852-23854	IN	denotes	on
T7538	23855-23864	RB	denotes	naturally
T7539	23865-23874	VBG	denotes	occurring
T7541	23875-23879	NN	denotes	cell
T7540	23880-23885	NN	denotes	death
T7542	23886-23888	FW	denotes	in
T7543	23889-23893	FW	denotes	vivo
T7544	23894-23899	VBG	denotes	using
T7545	23900-23905	NN	denotes	TUNEL
T7546	23906-23908	IN	denotes	on
T7547	23909-23912	NN	denotes	DRG
T7548	23913-23921	NNS	denotes	sections
T7549	23921-23922	.	denotes	.
T7550	23922-24130	sentence	denotes	Surprisingly, we found that apoptosis was decreased by approximately 50% (n = 3 embryos, average of >50 sections) in DRG of TauEWS-Pea3/+ Isl1Cre/+ embryos in comparison to wild-type (Figure 6D, 6J, and 6M).
T7551	23923-23935	RB	denotes	Surprisingly
T7553	23935-23937	,	denotes	,
T7554	23937-23939	PRP	denotes	we
T7552	23940-23945	VBD	denotes	found
T7555	23946-23950	IN	denotes	that
T7557	23951-23960	NN	denotes	apoptosis
T7558	23961-23964	VBD	denotes	was
T7556	23965-23974	VBN	denotes	decreased
T7559	23975-23977	IN	denotes	by
T7560	23978-23991	RB	denotes	approximately
T7561	23992-23994	CD	denotes	50
T7562	23994-23995	NN	denotes	%
T7563	23996-23997	-LRB-	denotes	(
T7565	23997-23998	NN	denotes	n
T7567	23999-24000	SYM	denotes	=
T7568	24001-24002	CD	denotes	3
T7566	24003-24010	NNS	denotes	embryos
T7569	24010-24012	,	denotes	,
T7564	24012-24019	NN	denotes	average
T7570	24020-24022	IN	denotes	of
T7571	24023-24024	SYM	denotes	>
T7572	24024-24026	CD	denotes	50
T7573	24027-24035	NNS	denotes	sections
T7574	24035-24036	-RRB-	denotes	)
T7575	24037-24039	IN	denotes	in
T7576	24040-24043	NN	denotes	DRG
T7577	24044-24046	IN	denotes	of
T7578	24047-24053	NN	denotes	TauEWS
T7580	24053-24054	HYPH	denotes	-
T7581	24054-24058	NN	denotes	Pea3
T7582	24058-24059	HYPH	denotes	/
T7583	24059-24060	SYM	denotes	+
T7579	24061-24068	NN	denotes	Isl1Cre
T7585	24068-24069	HYPH	denotes	/
T7586	24069-24070	SYM	denotes	+
T7584	24071-24078	NNS	denotes	embryos
T7587	24079-24081	IN	denotes	in
T7588	24082-24092	NN	denotes	comparison
T7589	24093-24095	IN	denotes	to
T7590	24096-24100	JJ	denotes	wild
T7592	24100-24101	HYPH	denotes	-
T7591	24101-24105	NN	denotes	type
T7593	24106-24107	-LRB-	denotes	(
T7595	24107-24113	NN	denotes	Figure
T7594	24114-24116	NN	denotes	6D
T7596	24116-24118	,	denotes	,
T7597	24118-24120	NN	denotes	6J
T7598	24120-24122	,	denotes	,
T7599	24122-24125	CC	denotes	and
T7600	24126-24128	NN	denotes	6M
T7601	24128-24129	-RRB-	denotes	)
T7602	24129-24130	.	denotes	.
T7603	24130-24325	sentence	denotes	Quantitative analysis of the number of neurons in lumbar DRG of TauEWS-Pea3/+ Isl1Cre/+ embryos revealed a significant increase to approximately 170% of wild-type levels (Figure 6E, 6K, and 6N).
T7604	24131-24143	JJ	denotes	Quantitative
T7605	24144-24152	NN	denotes	analysis
T7607	24153-24155	IN	denotes	of
T7608	24156-24159	DT	denotes	the
T7609	24160-24166	NN	denotes	number
T7610	24167-24169	IN	denotes	of
T7611	24170-24177	NNS	denotes	neurons
T7612	24178-24180	IN	denotes	in
T7613	24181-24187	NN	denotes	lumbar
T7614	24188-24191	NN	denotes	DRG
T7615	24192-24194	IN	denotes	of
T7616	24195-24201	NN	denotes	TauEWS
T7618	24201-24202	HYPH	denotes	-
T7619	24202-24206	NN	denotes	Pea3
T7620	24206-24207	HYPH	denotes	/
T7621	24207-24208	SYM	denotes	+
T7617	24209-24216	NN	denotes	Isl1Cre
T7623	24216-24217	HYPH	denotes	/
T7624	24217-24218	SYM	denotes	+
T7622	24219-24226	NNS	denotes	embryos
T7606	24227-24235	VBD	denotes	revealed
T7625	24236-24237	DT	denotes	a
T7627	24238-24249	JJ	denotes	significant
T7626	24250-24258	NN	denotes	increase
T7628	24259-24261	IN	denotes	to
T7629	24262-24275	RB	denotes	approximately
T7630	24276-24279	CD	denotes	170
T7631	24279-24280	NN	denotes	%
T7632	24281-24283	IN	denotes	of
T7633	24284-24288	JJ	denotes	wild
T7635	24288-24289	HYPH	denotes	-
T7634	24289-24293	NN	denotes	type
T7636	24294-24300	NNS	denotes	levels
T7637	24301-24302	-LRB-	denotes	(
T7639	24302-24308	NN	denotes	Figure
T7638	24309-24311	NN	denotes	6E
T7640	24311-24313	,	denotes	,
T7641	24313-24315	NN	denotes	6K
T7642	24315-24317	,	denotes	,
T7643	24317-24320	CC	denotes	and
T7644	24321-24323	NN	denotes	6N
T7645	24323-24324	-RRB-	denotes	)
T7646	24324-24325	.	denotes	.
T7647	24325-24553	sentence	denotes	Moreover, BrdU pulse-chase experiments ruled out the possibility that DRG neurons in TauEWS-Pea3/+ Isl1Cre/+ embryos reenter the cell cycle (no BrdU+/LacZ+ cells, n = 3 embryos, analysis of >50 sections each; Figure 6F and 6L).
T7648	24326-24334	RB	denotes	Moreover
T7650	24334-24336	,	denotes	,
T7651	24336-24340	NN	denotes	BrdU
T7653	24341-24346	NN	denotes	pulse
T7655	24346-24347	HYPH	denotes	-
T7654	24347-24352	NN	denotes	chase
T7652	24353-24364	NNS	denotes	experiments
T7649	24365-24370	VBD	denotes	ruled
T7656	24371-24374	RP	denotes	out
T7657	24375-24378	DT	denotes	the
T7658	24379-24390	NN	denotes	possibility
T7659	24391-24395	IN	denotes	that
T7661	24396-24399	NN	denotes	DRG
T7662	24400-24407	NNS	denotes	neurons
T7663	24408-24410	IN	denotes	in
T7664	24411-24417	NN	denotes	TauEWS
T7666	24417-24418	HYPH	denotes	-
T7667	24418-24422	NN	denotes	Pea3
T7668	24422-24423	HYPH	denotes	/
T7669	24423-24424	SYM	denotes	+
T7665	24425-24432	NN	denotes	Isl1Cre
T7671	24432-24433	HYPH	denotes	/
T7672	24433-24434	SYM	denotes	+
T7670	24435-24442	NNS	denotes	embryos
T7660	24443-24450	VBP	denotes	reenter
T7673	24451-24454	DT	denotes	the
T7675	24455-24459	NN	denotes	cell
T7674	24460-24465	NN	denotes	cycle
T7676	24466-24467	-LRB-	denotes	(
T7678	24467-24469	DT	denotes	no
T7680	24470-24474	NN	denotes	BrdU
T7682	24474-24475	SYM	denotes	+
T7683	24475-24476	HYPH	denotes	/
T7681	24476-24480	NN	denotes	LacZ
T7684	24480-24481	SYM	denotes	+
T7679	24482-24487	NNS	denotes	cells
T7685	24487-24489	,	denotes	,
T7686	24489-24490	NN	denotes	n
T7688	24491-24492	SYM	denotes	=
T7689	24493-24494	CD	denotes	3
T7687	24495-24502	NNS	denotes	embryos
T7690	24502-24504	,	denotes	,
T7691	24504-24512	NN	denotes	analysis
T7692	24513-24515	IN	denotes	of
T7693	24516-24517	SYM	denotes	>
T7694	24517-24519	CD	denotes	50
T7695	24520-24528	NNS	denotes	sections
T7696	24529-24533	RB	denotes	each
T7697	24533-24534	:	denotes	;
T7698	24535-24541	NN	denotes	Figure
T7677	24542-24544	NN	denotes	6F
T7699	24545-24548	CC	denotes	and
T7700	24549-24551	NN	denotes	6L
T7701	24551-24552	-RRB-	denotes	)
T7702	24552-24553	.	denotes	.
T7703	24553-24832	sentence	denotes	Together with the in vitro culture experiments, these findings suggest that DRG neurons from TauEWS-Pea3/+ Isl1Cre/+ embryos remain post-mitotic but fail to become sensitive to naturally occurring cell death, and survive in the absence of Trk receptors and neurotrophic support.
T7704	24554-24562	RB	denotes	Together
T7706	24563-24567	IN	denotes	with
T7707	24568-24571	DT	denotes	the
T7709	24572-24574	FW	denotes	in
T7710	24575-24580	FW	denotes	vitro
T7711	24581-24588	NN	denotes	culture
T7708	24589-24600	NNS	denotes	experiments
T7712	24600-24602	,	denotes	,
T7713	24602-24607	DT	denotes	these
T7714	24608-24616	NNS	denotes	findings
T7705	24617-24624	VBP	denotes	suggest
T7715	24625-24629	IN	denotes	that
T7717	24630-24633	NN	denotes	DRG
T7718	24634-24641	NNS	denotes	neurons
T7719	24642-24646	IN	denotes	from
T7720	24647-24653	NN	denotes	TauEWS
T7722	24653-24654	HYPH	denotes	-
T7723	24654-24658	NN	denotes	Pea3
T7724	24658-24659	HYPH	denotes	/
T7725	24659-24660	SYM	denotes	+
T7721	24661-24668	NN	denotes	Isl1Cre
T7727	24668-24669	HYPH	denotes	/
T7728	24669-24670	SYM	denotes	+
T7726	24671-24678	NNS	denotes	embryos
T7716	24679-24685	VBP	denotes	remain
T7729	24686-24698	JJ	denotes	post-mitotic
T7730	24699-24702	CC	denotes	but
T7731	24703-24707	VBP	denotes	fail
T7732	24708-24710	TO	denotes	to
T7733	24711-24717	VB	denotes	become
T7734	24718-24727	JJ	denotes	sensitive
T7735	24728-24730	IN	denotes	to
T7736	24731-24740	RB	denotes	naturally
T7737	24741-24750	VBG	denotes	occurring
T7739	24751-24755	NN	denotes	cell
T7738	24756-24761	NN	denotes	death
T7740	24761-24763	,	denotes	,
T7741	24763-24766	CC	denotes	and
T7742	24767-24774	VBP	denotes	survive
T7743	24775-24777	IN	denotes	in
T7744	24778-24781	DT	denotes	the
T7745	24782-24789	NN	denotes	absence
T7746	24790-24792	IN	denotes	of
T7747	24793-24796	NN	denotes	Trk
T7748	24797-24806	NNS	denotes	receptors
T7749	24807-24810	CC	denotes	and
T7750	24811-24823	JJ	denotes	neurotrophic
T7751	24824-24831	NN	denotes	support
T7752	24831-24832	.	denotes	.
T7753	24832-25030	sentence	denotes	We next analyzed whether changes in the expression of proteins known to be involved in the regulation of neuronal survival or cell death could be detected in DRG of TauEWS-Pea3/+ Isl1Cre/+ embryos.
T7754	24833-24835	PRP	denotes	We
T7756	24836-24840	RB	denotes	next
T7755	24841-24849	VBD	denotes	analyzed
T7757	24850-24857	IN	denotes	whether
T7759	24858-24865	NNS	denotes	changes
T7760	24866-24868	IN	denotes	in
T7761	24869-24872	DT	denotes	the
T7762	24873-24883	NN	denotes	expression
T7763	24884-24886	IN	denotes	of
T7764	24887-24895	NN	denotes	proteins
T7765	24896-24901	VBN	denotes	known
T7766	24902-24904	TO	denotes	to
T7768	24905-24907	VB	denotes	be
T7767	24908-24916	VBN	denotes	involved
T7769	24917-24919	IN	denotes	in
T7770	24920-24923	DT	denotes	the
T7771	24924-24934	NN	denotes	regulation
T7772	24935-24937	IN	denotes	of
T7773	24938-24946	JJ	denotes	neuronal
T7774	24947-24955	NN	denotes	survival
T7775	24956-24958	CC	denotes	or
T7776	24959-24963	NN	denotes	cell
T7777	24964-24969	NN	denotes	death
T7778	24970-24975	MD	denotes	could
T7779	24976-24978	VB	denotes	be
T7758	24979-24987	VBN	denotes	detected
T7780	24988-24990	IN	denotes	in
T7781	24991-24994	NN	denotes	DRG
T7782	24995-24997	IN	denotes	of
T7783	24998-25004	NN	denotes	TauEWS
T7785	25004-25005	HYPH	denotes	-
T7786	25005-25009	NN	denotes	Pea3
T7787	25009-25010	HYPH	denotes	/
T7788	25010-25011	SYM	denotes	+
T7784	25012-25019	NN	denotes	Isl1Cre
T7790	25019-25020	HYPH	denotes	/
T7791	25020-25021	SYM	denotes	+
T7789	25022-25029	NNS	denotes	embryos
T7792	25029-25030	.	denotes	.
T7793	25030-25220	sentence	denotes	We found no significant quantitative changes in the level of Akt/p-Akt or CREB/p-CREB in DRG (Figure 6O and 6P) both of which have been shown to be key regulators of neuronal survival [29].
T7794	25031-25033	PRP	denotes	We
T7795	25034-25039	VBD	denotes	found
T7796	25040-25042	DT	denotes	no
T7798	25043-25054	JJ	denotes	significant
T7799	25055-25067	JJ	denotes	quantitative
T7797	25068-25075	NNS	denotes	changes
T7800	25076-25078	IN	denotes	in
T7801	25079-25082	DT	denotes	the
T7802	25083-25088	NN	denotes	level
T7803	25089-25091	IN	denotes	of
T7804	25092-25095	NN	denotes	Akt
T7806	25095-25096	HYPH	denotes	/
T7807	25096-25097	NN	denotes	p
T7808	25097-25098	HYPH	denotes	-
T7805	25098-25101	NN	denotes	Akt
T7809	25102-25104	CC	denotes	or
T7810	25105-25109	NN	denotes	CREB
T7812	25109-25110	HYPH	denotes	/
T7813	25110-25111	NN	denotes	p
T7814	25111-25112	HYPH	denotes	-
T7811	25112-25116	NN	denotes	CREB
T7815	25117-25119	IN	denotes	in
T7816	25120-25123	NN	denotes	DRG
T7817	25124-25125	-LRB-	denotes	(
T7819	25125-25131	NN	denotes	Figure
T7818	25132-25134	NN	denotes	6O
T7820	25135-25138	CC	denotes	and
T7821	25139-25141	NN	denotes	6P
T7822	25141-25142	-RRB-	denotes	)
T7823	25143-25147	DT	denotes	both
T7825	25148-25150	IN	denotes	of
T7826	25151-25156	WDT	denotes	which
T7827	25157-25161	VBP	denotes	have
T7828	25162-25166	VBN	denotes	been
T7824	25167-25172	VBN	denotes	shown
T7829	25173-25175	TO	denotes	to
T7830	25176-25178	VB	denotes	be
T7831	25179-25182	JJ	denotes	key
T7832	25183-25193	NNS	denotes	regulators
T7833	25194-25196	IN	denotes	of
T7834	25197-25205	JJ	denotes	neuronal
T7835	25206-25214	NN	denotes	survival
T7836	25215-25216	-LRB-	denotes	[
T7837	25216-25218	CD	denotes	29
T7838	25218-25219	-RRB-	denotes	]
T7839	25219-25220	.	denotes	.
T7840	25220-25333	sentence	denotes	Moreover, the level of the proapoptotic Bcl2 family member Bax was not significantly reduced (Figure 6O and 6P).
T7841	25221-25229	RB	denotes	Moreover
T7843	25229-25231	,	denotes	,
T7844	25231-25234	DT	denotes	the
T7845	25235-25240	NN	denotes	level
T7846	25241-25243	IN	denotes	of
T7847	25244-25247	DT	denotes	the
T7849	25248-25260	JJ	denotes	proapoptotic
T7850	25261-25265	NN	denotes	Bcl2
T7851	25266-25272	NN	denotes	family
T7852	25273-25279	NN	denotes	member
T7848	25280-25283	NN	denotes	Bax
T7853	25284-25287	VBD	denotes	was
T7854	25288-25291	RB	denotes	not
T7855	25292-25305	RB	denotes	significantly
T7842	25306-25313	VBN	denotes	reduced
T7856	25314-25315	-LRB-	denotes	(
T7858	25315-25321	NN	denotes	Figure
T7857	25322-25324	NN	denotes	6O
T7859	25325-25328	CC	denotes	and
T7860	25329-25331	NN	denotes	6P
T7861	25331-25332	-RRB-	denotes	)
T7862	25332-25333	.	denotes	.
T7863	25333-25752	sentence	denotes	In contrast, the expression level of the anti-apoptotic Bcl2 family members Bcl-xl and Bcl2 was significantly increased when compared to wild-type levels (Bcl2, 157%; Bcl-xl, 259%; average of n = 3 independent experiments; Figure 6O and 6P), providing a potential molecular explanation for the enhanced neuronal survival of DRG neurons of TauEWS-Pea3/+ Isl1Cre/+ embryos in the absence of Trk receptor expression [30].
T7864	25334-25336	IN	denotes	In
T7866	25337-25345	NN	denotes	contrast
T7867	25345-25347	,	denotes	,
T7868	25347-25350	DT	denotes	the
T7870	25351-25361	NN	denotes	expression
T7869	25362-25367	NN	denotes	level
T7871	25368-25370	IN	denotes	of
T7872	25371-25374	DT	denotes	the
T7874	25375-25389	JJ	denotes	anti-apoptotic
T7875	25390-25394	NN	denotes	Bcl2
T7876	25395-25401	NN	denotes	family
T7873	25402-25409	NNS	denotes	members
T7877	25410-25413	NN	denotes	Bcl
T7879	25413-25414	HYPH	denotes	-
T7878	25414-25416	NN	denotes	xl
T7880	25417-25420	CC	denotes	and
T7881	25421-25425	NN	denotes	Bcl2
T7882	25426-25429	VBD	denotes	was
T7883	25430-25443	RB	denotes	significantly
T7865	25444-25453	VBN	denotes	increased
T7884	25454-25458	WRB	denotes	when
T7885	25459-25467	VBN	denotes	compared
T7886	25468-25470	IN	denotes	to
T7887	25471-25475	JJ	denotes	wild
T7889	25475-25476	HYPH	denotes	-
T7888	25476-25480	NN	denotes	type
T7890	25481-25487	NNS	denotes	levels
T7891	25488-25489	-LRB-	denotes	(
T7893	25489-25493	NN	denotes	Bcl2
T7895	25493-25495	,	denotes	,
T7896	25495-25498	CD	denotes	157
T7894	25498-25499	NN	denotes	%
T7897	25499-25500	:	denotes	;
T7898	25501-25504	NN	denotes	Bcl
T7900	25504-25505	HYPH	denotes	-
T7899	25505-25507	NN	denotes	xl
T7902	25507-25509	,	denotes	,
T7903	25509-25512	CD	denotes	259
T7901	25512-25513	NN	denotes	%
T7904	25513-25514	:	denotes	;
T7905	25515-25522	NN	denotes	average
T7906	25523-25525	IN	denotes	of
T7907	25526-25527	NN	denotes	n
T7909	25528-25529	SYM	denotes	=
T7910	25530-25531	CD	denotes	3
T7911	25532-25543	JJ	denotes	independent
T7908	25544-25555	NNS	denotes	experiments
T7912	25555-25556	:	denotes	;
T7913	25557-25563	NN	denotes	Figure
T7892	25564-25566	NN	denotes	6O
T7914	25567-25570	CC	denotes	and
T7915	25571-25573	NN	denotes	6P
T7916	25573-25574	-RRB-	denotes	)
T7917	25574-25576	,	denotes	,
T7918	25576-25585	VBG	denotes	providing
T7919	25586-25587	DT	denotes	a
T7921	25588-25597	JJ	denotes	potential
T7922	25598-25607	JJ	denotes	molecular
T7920	25608-25619	NN	denotes	explanation
T7923	25620-25623	IN	denotes	for
T7924	25624-25627	DT	denotes	the
T7926	25628-25636	VBN	denotes	enhanced
T7927	25637-25645	JJ	denotes	neuronal
T7925	25646-25654	NN	denotes	survival
T7928	25655-25657	IN	denotes	of
T7929	25658-25661	NN	denotes	DRG
T7930	25662-25669	NNS	denotes	neurons
T7931	25670-25672	IN	denotes	of
T7932	25673-25679	NN	denotes	TauEWS
T7934	25679-25680	HYPH	denotes	-
T7935	25680-25684	NN	denotes	Pea3
T7936	25684-25685	HYPH	denotes	/
T7937	25685-25686	SYM	denotes	+
T7933	25687-25694	NN	denotes	Isl1Cre
T7939	25694-25695	HYPH	denotes	/
T7940	25695-25696	SYM	denotes	+
T7938	25697-25704	NNS	denotes	embryos
T7941	25705-25707	IN	denotes	in
T7942	25708-25711	DT	denotes	the
T7943	25712-25719	NN	denotes	absence
T7944	25720-25722	IN	denotes	of
T7945	25723-25726	NN	denotes	Trk
T7946	25727-25735	NN	denotes	receptor
T7947	25736-25746	NN	denotes	expression
T7948	25747-25748	-LRB-	denotes	[
T7949	25748-25750	CD	denotes	30
T7950	25750-25751	-RRB-	denotes	]
T7951	25751-25752	.	denotes	.
T8705	25754-25758	RB	denotes	Only
T8707	25759-25769	JJ	denotes	Precocious
T8708	25770-25773	CC	denotes	but
T8709	25774-25777	RB	denotes	Not
T8710	25778-25788	JJ	denotes	Isochronic
T8711	25789-25792	NN	denotes	ETS
T8706	25793-25802	NN	denotes	Signaling
T8713	25803-25805	IN	denotes	in
T8714	25806-25809	NN	denotes	DRG
T8715	25810-25817	NNS	denotes	Neurons
T8712	25818-25828	VBZ	denotes	Interferes
T8716	25829-25833	IN	denotes	with
T8717	25834-25842	JJ	denotes	Neuronal
T8718	25843-25847	NN	denotes	Fate
T8719	25848-25859	NN	denotes	Acquisition
T8720	25859-26236	sentence	denotes	The observed differences in neuronal survival and neurite outgrowth between precocious and isochronic expression of EWS-Pea3 prompted us to perform a direct comparative analysis of gene expression between mice with precocious EWS-Pea3 expression and mice in which the expression of EWS-Pea3 is initiated in DRG sensory neurons from the time of normal onset of Er81 expression.
T8721	25860-25863	DT	denotes	The
T8723	25864-25872	VBN	denotes	observed
T8722	25873-25884	NNS	denotes	differences
T8725	25885-25887	IN	denotes	in
T8726	25888-25896	JJ	denotes	neuronal
T8727	25897-25905	NN	denotes	survival
T8728	25906-25909	CC	denotes	and
T8729	25910-25917	NN	denotes	neurite
T8730	25918-25927	NN	denotes	outgrowth
T8731	25928-25935	IN	denotes	between
T8732	25936-25946	JJ	denotes	precocious
T8734	25947-25950	CC	denotes	and
T8735	25951-25961	JJ	denotes	isochronic
T8733	25962-25972	NN	denotes	expression
T8736	25973-25975	IN	denotes	of
T8737	25976-25979	NN	denotes	EWS
T8739	25979-25980	HYPH	denotes	-
T8738	25980-25984	NN	denotes	Pea3
T8724	25985-25993	VBD	denotes	prompted
T8740	25994-25996	PRP	denotes	us
T8741	25997-25999	TO	denotes	to
T8742	26000-26007	VB	denotes	perform
T8743	26008-26009	DT	denotes	a
T8745	26010-26016	JJ	denotes	direct
T8746	26017-26028	JJ	denotes	comparative
T8744	26029-26037	NN	denotes	analysis
T8747	26038-26040	IN	denotes	of
T8748	26041-26045	NN	denotes	gene
T8749	26046-26056	NN	denotes	expression
T8750	26057-26064	IN	denotes	between
T8751	26065-26069	NNS	denotes	mice
T8752	26070-26074	IN	denotes	with
T8753	26075-26085	JJ	denotes	precocious
T8755	26086-26089	NN	denotes	EWS
T8757	26089-26090	HYPH	denotes	-
T8756	26090-26094	NN	denotes	Pea3
T8754	26095-26105	NN	denotes	expression
T8758	26106-26109	CC	denotes	and
T8759	26110-26114	NNS	denotes	mice
T8760	26115-26117	IN	denotes	in
T8762	26118-26123	WDT	denotes	which
T8763	26124-26127	DT	denotes	the
T8764	26128-26138	NN	denotes	expression
T8765	26139-26141	IN	denotes	of
T8766	26142-26145	NN	denotes	EWS
T8768	26145-26146	HYPH	denotes	-
T8767	26146-26150	NN	denotes	Pea3
T8769	26151-26153	VBZ	denotes	is
T8761	26154-26163	VBN	denotes	initiated
T8770	26164-26166	IN	denotes	in
T8771	26167-26170	NN	denotes	DRG
T8773	26171-26178	JJ	denotes	sensory
T8772	26179-26186	NNS	denotes	neurons
T8774	26187-26191	IN	denotes	from
T8775	26192-26195	DT	denotes	the
T8776	26196-26200	NN	denotes	time
T8777	26201-26203	IN	denotes	of
T8778	26204-26210	JJ	denotes	normal
T8779	26211-26216	NN	denotes	onset
T8780	26217-26219	IN	denotes	of
T8781	26220-26224	NN	denotes	Er81
T8782	26225-26235	NN	denotes	expression
T8783	26235-26236	.	denotes	.
T8784	26236-26506	sentence	denotes	Moreover, to rule out the possibility that a differential effect may be due to the different genetic strategies by which expression of EWS-Pea3 in proprioceptive afferents is achieved, we performed this analysis both in Er81EWS-Pea3/− and TauEWS-Pea3/+ PVCre/+ embryos.
T8785	26237-26245	RB	denotes	Moreover
T8787	26245-26247	,	denotes	,
T8788	26247-26249	TO	denotes	to
T8789	26250-26254	VB	denotes	rule
T8790	26255-26258	RP	denotes	out
T8791	26259-26262	DT	denotes	the
T8792	26263-26274	NN	denotes	possibility
T8793	26275-26279	IN	denotes	that
T8795	26280-26281	DT	denotes	a
T8797	26282-26294	JJ	denotes	differential
T8796	26295-26301	NN	denotes	effect
T8798	26302-26305	MD	denotes	may
T8794	26306-26308	VB	denotes	be
T8799	26309-26312	IN	denotes	due
T8800	26313-26315	IN	denotes	to
T8801	26316-26319	DT	denotes	the
T8803	26320-26329	JJ	denotes	different
T8804	26330-26337	JJ	denotes	genetic
T8802	26338-26348	NNS	denotes	strategies
T8805	26349-26351	IN	denotes	by
T8807	26352-26357	WDT	denotes	which
T8808	26358-26368	NN	denotes	expression
T8809	26369-26371	IN	denotes	of
T8810	26372-26375	NN	denotes	EWS
T8812	26375-26376	HYPH	denotes	-
T8811	26376-26380	NN	denotes	Pea3
T8813	26381-26383	IN	denotes	in
T8814	26384-26398	JJ	denotes	proprioceptive
T8815	26399-26408	NNS	denotes	afferents
T8816	26409-26411	VBZ	denotes	is
T8806	26412-26420	VBN	denotes	achieved
T8817	26420-26422	,	denotes	,
T8818	26422-26424	PRP	denotes	we
T8786	26425-26434	VBD	denotes	performed
T8819	26435-26439	DT	denotes	this
T8820	26440-26448	NN	denotes	analysis
T8821	26449-26453	CC	denotes	both
T8822	26454-26456	IN	denotes	in
T8823	26457-26464	NN	denotes	Er81EWS
T8825	26464-26465	HYPH	denotes	-
T8824	26465-26469	NN	denotes	Pea3
T8827	26469-26470	HYPH	denotes	/
T8828	26470-26471	SYM	denotes	−
T8829	26472-26475	CC	denotes	and
T8830	26476-26482	NN	denotes	TauEWS
T8832	26482-26483	HYPH	denotes	-
T8833	26483-26487	NN	denotes	Pea3
T8834	26487-26488	HYPH	denotes	/
T8835	26488-26489	SYM	denotes	+
T8831	26490-26495	NN	denotes	PVCre
T8836	26495-26496	HYPH	denotes	/
T8837	26496-26497	SYM	denotes	+
T8826	26498-26505	NNS	denotes	embryos
T8838	26505-26506	.	denotes	.
T8839	26506-26635	sentence	denotes	We first analyzed expression of TrkC, a gene downregulated in DRG neurons of TauEWS-Pea3/+ Isl1Cre/+ embryos (Figure 7A and 7B).
T8840	26507-26509	PRP	denotes	We
T8842	26510-26515	RB	denotes	first
T8841	26516-26524	VBD	denotes	analyzed
T8843	26525-26535	NN	denotes	expression
T8844	26536-26538	IN	denotes	of
T8845	26539-26543	NN	denotes	TrkC
T8846	26543-26545	,	denotes	,
T8847	26545-26546	DT	denotes	a
T8848	26547-26551	NN	denotes	gene
T8849	26552-26565	VBN	denotes	downregulated
T8850	26566-26568	IN	denotes	in
T8851	26569-26572	NN	denotes	DRG
T8852	26573-26580	NNS	denotes	neurons
T8853	26581-26583	IN	denotes	of
T8854	26584-26590	NN	denotes	TauEWS
T8856	26590-26591	HYPH	denotes	-
T8857	26591-26595	NN	denotes	Pea3
T8858	26595-26596	HYPH	denotes	/
T8859	26596-26597	SYM	denotes	+
T8855	26598-26605	NN	denotes	Isl1Cre
T8861	26605-26606	HYPH	denotes	/
T8862	26606-26607	SYM	denotes	+
T8860	26608-26615	NNS	denotes	embryos
T8863	26616-26617	-LRB-	denotes	(
T8865	26617-26623	NN	denotes	Figure
T8864	26624-26626	NN	denotes	7A
T8866	26627-26630	CC	denotes	and
T8867	26631-26633	NN	denotes	7B
T8868	26633-26634	-RRB-	denotes	)
T8869	26634-26635	.	denotes	.
T8870	26635-26796	sentence	denotes	The level of expression of TrkC was indistinguishable from wild-type in DRG neurons of Er81EWS-Pea3/− and TauEWS-Pea3/+ PVCre/+ embryos (Figure 7A, 7C, and 7D).
T8871	26636-26639	DT	denotes	The
T8872	26640-26645	NN	denotes	level
T8874	26646-26648	IN	denotes	of
T8875	26649-26659	NN	denotes	expression
T8876	26660-26662	IN	denotes	of
T8877	26663-26667	NN	denotes	TrkC
T8873	26668-26671	VBD	denotes	was
T8878	26672-26689	JJ	denotes	indistinguishable
T8879	26690-26694	IN	denotes	from
T8880	26695-26699	JJ	denotes	wild
T8882	26699-26700	HYPH	denotes	-
T8881	26700-26704	NN	denotes	type
T8883	26705-26707	IN	denotes	in
T8884	26708-26711	NN	denotes	DRG
T8885	26712-26719	NNS	denotes	neurons
T8886	26720-26722	IN	denotes	of
T8887	26723-26730	NN	denotes	Er81EWS
T8889	26730-26731	HYPH	denotes	-
T8888	26731-26735	NN	denotes	Pea3
T8891	26735-26736	HYPH	denotes	/
T8892	26736-26737	SYM	denotes	−
T8893	26738-26741	CC	denotes	and
T8894	26742-26748	NN	denotes	TauEWS
T8896	26748-26749	HYPH	denotes	-
T8897	26749-26753	NN	denotes	Pea3
T8898	26753-26754	HYPH	denotes	/
T8899	26754-26755	SYM	denotes	+
T8895	26756-26761	NN	denotes	PVCre
T8900	26761-26762	HYPH	denotes	/
T8901	26762-26763	SYM	denotes	+
T8890	26764-26771	NNS	denotes	embryos
T8902	26772-26773	-LRB-	denotes	(
T8904	26773-26779	NN	denotes	Figure
T8903	26780-26782	NN	denotes	7A
T8905	26782-26784	,	denotes	,
T8906	26784-26786	NN	denotes	7C
T8907	26786-26788	,	denotes	,
T8908	26788-26791	CC	denotes	and
T8909	26792-26794	NN	denotes	7D
T8910	26794-26795	-RRB-	denotes	)
T8911	26795-26796	.	denotes	.
T8912	26796-27009	sentence	denotes	Moreover, PV was not expressed in DRG neurons of TauEWS-Pea3/+ Isl1Cre/+ embryos (Figure S5) but was expressed by proprioceptive afferents in both wild-type and Er81EWS-Pea3/− embryos (see Figures 1 and S5) [14].
T8913	26797-26805	RB	denotes	Moreover
T8915	26805-26807	,	denotes	,
T8916	26807-26809	NN	denotes	PV
T8917	26810-26813	VBD	denotes	was
T8918	26814-26817	RB	denotes	not
T8914	26818-26827	VBN	denotes	expressed
T8919	26828-26830	IN	denotes	in
T8920	26831-26834	NN	denotes	DRG
T8921	26835-26842	NNS	denotes	neurons
T8922	26843-26845	IN	denotes	of
T8923	26846-26852	NN	denotes	TauEWS
T8925	26852-26853	HYPH	denotes	-
T8926	26853-26857	NN	denotes	Pea3
T8927	26857-26858	HYPH	denotes	/
T8928	26858-26859	SYM	denotes	+
T8924	26860-26867	NN	denotes	Isl1Cre
T8930	26867-26868	HYPH	denotes	/
T8931	26868-26869	SYM	denotes	+
T8929	26870-26877	NNS	denotes	embryos
T8932	26878-26879	-LRB-	denotes	(
T8934	26879-26885	NN	denotes	Figure
T8933	26886-26888	NN	denotes	S5
T8935	26888-26889	-RRB-	denotes	)
T8936	26890-26893	CC	denotes	but
T8937	26894-26897	VBD	denotes	was
T8938	26898-26907	VBN	denotes	expressed
T8939	26908-26910	IN	denotes	by
T8940	26911-26925	JJ	denotes	proprioceptive
T8941	26926-26935	NNS	denotes	afferents
T8942	26936-26938	IN	denotes	in
T8943	26939-26943	CC	denotes	both
T8945	26944-26948	JJ	denotes	wild
T8946	26948-26949	HYPH	denotes	-
T8944	26949-26953	NN	denotes	type
T8948	26954-26957	CC	denotes	and
T8949	26958-26965	NN	denotes	Er81EWS
T8951	26965-26966	HYPH	denotes	-
T8950	26966-26970	NN	denotes	Pea3
T8952	26970-26971	HYPH	denotes	/
T8953	26971-26972	SYM	denotes	−
T8947	26973-26980	NNS	denotes	embryos
T8954	26981-26982	-LRB-	denotes	(
T8955	26982-26985	VB	denotes	see
T8956	26986-26993	NNS	denotes	Figures
T8958	26994-26995	CD	denotes	1
T8959	26996-26999	CC	denotes	and
T8957	27000-27002	NN	denotes	S5
T8960	27002-27003	-RRB-	denotes	)
T8961	27004-27005	-LRB-	denotes	[
T8962	27005-27007	CD	denotes	14
T8963	27007-27008	-RRB-	denotes	]
T8964	27008-27009	.	denotes	.
T8965	27009-27133	sentence	denotes	We also found several genes that were ectopically upregulated in DRG neurons of TauEWS-Pea3/+ Isl1Cre/+ embryos (Figure 7).
T8966	27010-27012	PRP	denotes	We
T8968	27013-27017	RB	denotes	also
T8967	27018-27023	VBD	denotes	found
T8969	27024-27031	JJ	denotes	several
T8970	27032-27037	NNS	denotes	genes
T8971	27038-27042	WDT	denotes	that
T8973	27043-27047	VBD	denotes	were
T8974	27048-27059	RB	denotes	ectopically
T8972	27060-27071	VBN	denotes	upregulated
T8975	27072-27074	IN	denotes	in
T8976	27075-27078	NN	denotes	DRG
T8977	27079-27086	NNS	denotes	neurons
T8978	27087-27089	IN	denotes	of
T8979	27090-27096	NN	denotes	TauEWS
T8981	27096-27097	HYPH	denotes	-
T8982	27097-27101	NN	denotes	Pea3
T8983	27101-27102	HYPH	denotes	/
T8984	27102-27103	SYM	denotes	+
T8980	27104-27111	NN	denotes	Isl1Cre
T8986	27111-27112	HYPH	denotes	/
T8987	27112-27113	SYM	denotes	+
T8985	27114-27121	NNS	denotes	embryos
T8988	27122-27123	-LRB-	denotes	(
T8989	27123-27129	NN	denotes	Figure
T8990	27130-27131	CD	denotes	7
T8991	27131-27132	-RRB-	denotes	)
T8992	27132-27133	.	denotes	.
T8993	27133-27459	sentence	denotes	Calretinin and Calbindin, two different calcium-binding proteins expressed by subpopulations of DRG neurons in wild-type, Er81EWS-Pea3/−, and TauEWS-Pea3/+ PVCre/+ embryos (Figure 7E, 7G, and 7H; data not shown) [31,32], were induced in more than 95% of all DRG neurons of TauEWS-Pea3/+ Isl1Cre/+ embryos (Figures 7F and S5).
T8994	27134-27144	NN	denotes	Calretinin
T8996	27145-27148	CC	denotes	and
T8997	27149-27158	NN	denotes	Calbindin
T8998	27158-27160	,	denotes	,
T8999	27160-27163	CD	denotes	two
T9001	27164-27173	JJ	denotes	different
T9002	27174-27181	NN	denotes	calcium
T9004	27181-27182	HYPH	denotes	-
T9003	27182-27189	VBG	denotes	binding
T9000	27190-27198	NN	denotes	proteins
T9005	27199-27208	VBN	denotes	expressed
T9006	27209-27211	IN	denotes	by
T9007	27212-27226	NNS	denotes	subpopulations
T9008	27227-27229	IN	denotes	of
T9009	27230-27233	NN	denotes	DRG
T9010	27234-27241	NNS	denotes	neurons
T9011	27242-27244	IN	denotes	in
T9012	27245-27249	JJ	denotes	wild
T9014	27249-27250	HYPH	denotes	-
T9013	27250-27254	NN	denotes	type
T9016	27254-27256	,	denotes	,
T9017	27256-27263	NN	denotes	Er81EWS
T9019	27263-27264	HYPH	denotes	-
T9018	27264-27268	NN	denotes	Pea3
T9020	27268-27269	HYPH	denotes	/
T9021	27269-27270	SYM	denotes	−
T9022	27270-27272	,	denotes	,
T9023	27272-27275	CC	denotes	and
T9024	27276-27282	NN	denotes	TauEWS
T9026	27282-27283	HYPH	denotes	-
T9027	27283-27287	NN	denotes	Pea3
T9028	27287-27288	HYPH	denotes	/
T9029	27288-27289	SYM	denotes	+
T9025	27290-27295	NN	denotes	PVCre
T9030	27295-27296	HYPH	denotes	/
T9031	27296-27297	SYM	denotes	+
T9015	27298-27305	NNS	denotes	embryos
T9032	27306-27307	-LRB-	denotes	(
T9034	27307-27313	NN	denotes	Figure
T9033	27314-27316	NN	denotes	7E
T9035	27316-27318	,	denotes	,
T9036	27318-27320	NN	denotes	7G
T9037	27320-27322	,	denotes	,
T9038	27322-27325	CC	denotes	and
T9039	27326-27328	NN	denotes	7H
T9040	27328-27329	:	denotes	;
T9041	27330-27334	NNS	denotes	data
T9043	27335-27338	RB	denotes	not
T9042	27339-27344	VBN	denotes	shown
T9044	27344-27345	-RRB-	denotes	)
T9045	27346-27347	-LRB-	denotes	[
T9047	27347-27349	CD	denotes	31
T9048	27349-27350	,	denotes	,
T9046	27350-27352	CD	denotes	32
T9049	27352-27353	-RRB-	denotes	]
T9050	27353-27355	,	denotes	,
T9051	27355-27359	VBD	denotes	were
T8995	27360-27367	VBN	denotes	induced
T9052	27368-27370	IN	denotes	in
T9053	27371-27375	JJR	denotes	more
T9055	27376-27380	IN	denotes	than
T9054	27381-27383	CD	denotes	95
T9056	27383-27384	NN	denotes	%
T9057	27385-27387	IN	denotes	of
T9058	27388-27391	DT	denotes	all
T9060	27392-27395	NN	denotes	DRG
T9059	27396-27403	NNS	denotes	neurons
T9061	27404-27406	IN	denotes	of
T9062	27407-27413	NN	denotes	TauEWS
T9064	27413-27414	HYPH	denotes	-
T9065	27414-27418	NN	denotes	Pea3
T9066	27418-27419	HYPH	denotes	/
T9067	27419-27420	SYM	denotes	+
T9063	27421-27428	NN	denotes	Isl1Cre
T9069	27428-27429	HYPH	denotes	/
T9070	27429-27430	SYM	denotes	+
T9068	27431-27438	NNS	denotes	embryos
T9071	27439-27440	-LRB-	denotes	(
T9073	27440-27447	NNS	denotes	Figures
T9072	27448-27450	NN	denotes	7F
T9074	27451-27454	CC	denotes	and
T9075	27455-27457	NN	denotes	S5
T9076	27457-27458	-RRB-	denotes	)
T9077	27458-27459	.	denotes	.
T9078	27459-27672	sentence	denotes	These findings suggest that DRG neurons in TauEWS-Pea3/+ Isl1Cre/+ embryos fail to differentiate to a normal fate and instead acquire an aberrant identity distinct from any subpopulation of wild-type DRG neurons.
T9079	27460-27465	DT	denotes	These
T9080	27466-27474	NNS	denotes	findings
T9081	27475-27482	VBP	denotes	suggest
T9082	27483-27487	IN	denotes	that
T9084	27488-27491	NN	denotes	DRG
T9085	27492-27499	NNS	denotes	neurons
T9086	27500-27502	IN	denotes	in
T9087	27503-27509	NN	denotes	TauEWS
T9089	27509-27510	HYPH	denotes	-
T9090	27510-27514	NN	denotes	Pea3
T9091	27514-27515	HYPH	denotes	/
T9092	27515-27516	SYM	denotes	+
T9088	27517-27524	NN	denotes	Isl1Cre
T9094	27524-27525	HYPH	denotes	/
T9095	27525-27526	SYM	denotes	+
T9093	27527-27534	NNS	denotes	embryos
T9083	27535-27539	VBP	denotes	fail
T9096	27540-27542	TO	denotes	to
T9097	27543-27556	VB	denotes	differentiate
T9098	27557-27559	IN	denotes	to
T9099	27560-27561	DT	denotes	a
T9101	27562-27568	JJ	denotes	normal
T9100	27569-27573	NN	denotes	fate
T9102	27574-27577	CC	denotes	and
T9103	27578-27585	RB	denotes	instead
T9104	27586-27593	VB	denotes	acquire
T9105	27594-27596	DT	denotes	an
T9107	27597-27605	JJ	denotes	aberrant
T9106	27606-27614	NN	denotes	identity
T9108	27615-27623	JJ	denotes	distinct
T9109	27624-27628	IN	denotes	from
T9110	27629-27632	DT	denotes	any
T9111	27633-27646	NN	denotes	subpopulation
T9112	27647-27649	IN	denotes	of
T9113	27650-27654	JJ	denotes	wild
T9115	27654-27655	HYPH	denotes	-
T9114	27655-27659	NN	denotes	type
T9117	27660-27663	NN	denotes	DRG
T9116	27664-27671	NNS	denotes	neurons
T9118	27671-27672	.	denotes	.
T9119	27672-27884	sentence	denotes	Finally, to assess whether EWS-Pea3 expressed precociously acts exclusively cell-autonomously or whether it may also influence neighboring DRG neurons, we activated expression of EWS-Pea3 using Hb9Cre mice [33].
T9120	27673-27680	RB	denotes	Finally
T9122	27680-27682	,	denotes	,
T9123	27682-27684	TO	denotes	to
T9124	27685-27691	VB	denotes	assess
T9125	27692-27699	IN	denotes	whether
T9127	27700-27703	NN	denotes	EWS
T9129	27703-27704	HYPH	denotes	-
T9128	27704-27708	NN	denotes	Pea3
T9130	27709-27718	VBN	denotes	expressed
T9131	27719-27731	RB	denotes	precociously
T9126	27732-27736	VBZ	denotes	acts
T9132	27737-27748	RB	denotes	exclusively
T9134	27749-27753	NN	denotes	cell
T9135	27753-27754	HYPH	denotes	-
T9133	27754-27766	RB	denotes	autonomously
T9136	27767-27769	CC	denotes	or
T9137	27770-27777	IN	denotes	whether
T9139	27778-27780	PRP	denotes	it
T9140	27781-27784	MD	denotes	may
T9141	27785-27789	RB	denotes	also
T9138	27790-27799	VB	denotes	influence
T9142	27800-27811	VBG	denotes	neighboring
T9144	27812-27815	NN	denotes	DRG
T9143	27816-27823	NNS	denotes	neurons
T9145	27823-27825	,	denotes	,
T9146	27825-27827	PRP	denotes	we
T9121	27828-27837	VBD	denotes	activated
T9147	27838-27848	NN	denotes	expression
T9148	27849-27851	IN	denotes	of
T9149	27852-27855	NN	denotes	EWS
T9151	27855-27856	HYPH	denotes	-
T9150	27856-27860	NN	denotes	Pea3
T9152	27861-27866	VBG	denotes	using
T9153	27867-27873	NN	denotes	Hb9Cre
T9154	27874-27878	NNS	denotes	mice
T9155	27879-27880	-LRB-	denotes	[
T9156	27880-27882	CD	denotes	33
T9157	27882-27883	-RRB-	denotes	]
T9158	27883-27884	.	denotes	.
T9159	27884-28154	sentence	denotes	Due to a transient and rostro-caudally graded expression of Hb9 at neural plate stages, very few DRG neurons at brachial levels and increasingly more neurons progressing caudally undergo recombination in TauEWS-Pea3/+ Hb9Cre/+ and TaumGFP/+ Hb9Cre/+ embryos (Figure 8).
T9160	27885-27888	IN	denotes	Due
T9162	27889-27891	IN	denotes	to
T9163	27892-27893	DT	denotes	a
T9165	27894-27903	JJ	denotes	transient
T9166	27904-27907	CC	denotes	and
T9167	27908-27914	RB	denotes	rostro
T9169	27914-27915	HYPH	denotes	-
T9168	27915-27923	RB	denotes	caudally
T9170	27924-27930	VBN	denotes	graded
T9164	27931-27941	NN	denotes	expression
T9171	27942-27944	IN	denotes	of
T9172	27945-27948	NN	denotes	Hb9
T9173	27949-27951	IN	denotes	at
T9174	27952-27958	JJ	denotes	neural
T9175	27959-27964	NN	denotes	plate
T9176	27965-27971	NNS	denotes	stages
T9177	27971-27973	,	denotes	,
T9178	27973-27977	RB	denotes	very
T9179	27978-27981	JJ	denotes	few
T9181	27982-27985	NN	denotes	DRG
T9180	27986-27993	NNS	denotes	neurons
T9182	27994-27996	IN	denotes	at
T9183	27997-28005	JJ	denotes	brachial
T9184	28006-28012	NNS	denotes	levels
T9185	28013-28016	CC	denotes	and
T9186	28017-28029	RB	denotes	increasingly
T9187	28030-28034	JJR	denotes	more
T9188	28035-28042	NNS	denotes	neurons
T9189	28043-28054	VBG	denotes	progressing
T9190	28055-28063	RB	denotes	caudally
T9161	28064-28071	VBP	denotes	undergo
T9191	28072-28085	NN	denotes	recombination
T9192	28086-28088	IN	denotes	in
T9193	28089-28095	NN	denotes	TauEWS
T9195	28095-28096	HYPH	denotes	-
T9196	28096-28100	NN	denotes	Pea3
T9197	28100-28101	HYPH	denotes	/
T9198	28101-28102	SYM	denotes	+
T9194	28103-28109	NN	denotes	Hb9Cre
T9200	28109-28110	HYPH	denotes	/
T9201	28110-28111	SYM	denotes	+
T9202	28112-28115	CC	denotes	and
T9203	28116-28123	NN	denotes	TaumGFP
T9205	28123-28124	HYPH	denotes	/
T9206	28124-28125	SYM	denotes	+
T9204	28126-28132	NN	denotes	Hb9Cre
T9207	28132-28133	HYPH	denotes	/
T9208	28133-28134	SYM	denotes	+
T9199	28135-28142	NNS	denotes	embryos
T9209	28143-28144	-LRB-	denotes	(
T9210	28144-28150	NN	denotes	Figure
T9211	28151-28152	CD	denotes	8
T9212	28152-28153	-RRB-	denotes	)
T9213	28153-28154	.	denotes	.
T9214	28154-28376	sentence	denotes	Nevertheless, downregulation of Trk receptor expression or upregulation of Calretinin is restricted exclusively to neurons that have undergone recombination and cannot be observed in TaumGFP/+ Hb9Cre/+ embryos (Figure 8).
T9215	28155-28167	RB	denotes	Nevertheless
T9217	28167-28169	,	denotes	,
T9218	28169-28183	NN	denotes	downregulation
T9219	28184-28186	IN	denotes	of
T9220	28187-28190	NN	denotes	Trk
T9222	28191-28199	NN	denotes	receptor
T9221	28200-28210	NN	denotes	expression
T9223	28211-28213	CC	denotes	or
T9224	28214-28226	NN	denotes	upregulation
T9225	28227-28229	IN	denotes	of
T9226	28230-28240	NN	denotes	Calretinin
T9227	28241-28243	VBZ	denotes	is
T9216	28244-28254	VBN	denotes	restricted
T9228	28255-28266	RB	denotes	exclusively
T9229	28267-28269	IN	denotes	to
T9230	28270-28277	NNS	denotes	neurons
T9231	28278-28282	WDT	denotes	that
T9233	28283-28287	VBP	denotes	have
T9232	28288-28297	VBN	denotes	undergone
T9234	28298-28311	NN	denotes	recombination
T9235	28312-28315	CC	denotes	and
T9236	28316-28319	MD	denotes	can
T9238	28319-28322	RB	denotes	not
T9239	28323-28325	VB	denotes	be
T9237	28326-28334	VBN	denotes	observed
T9240	28335-28337	IN	denotes	in
T9241	28338-28345	NN	denotes	TaumGFP
T9243	28345-28346	HYPH	denotes	/
T9244	28346-28347	SYM	denotes	+
T9242	28348-28354	NN	denotes	Hb9Cre
T9246	28354-28355	HYPH	denotes	/
T9247	28355-28356	SYM	denotes	+
T9245	28357-28364	NNS	denotes	embryos
T9248	28365-28366	-LRB-	denotes	(
T9249	28366-28372	NN	denotes	Figure
T9250	28373-28374	CD	denotes	8
T9251	28374-28375	-RRB-	denotes	)
T9252	28375-28376	.	denotes	.
T9253	28376-28721	sentence	denotes	Together, these results and the findings obtained from in vitro culture experiments (see Figures 4 and 5) demonstrate that precocious or isochronic expression of EWS-Pea3 in the same neurons leads to significantly different cell-autonomous cellular responses with respect to gene expression, neuronal survival, and neurite outgrowth (Figure 9).
T9254	28377-28385	RB	denotes	Together
T9256	28385-28387	,	denotes	,
T9257	28387-28392	DT	denotes	these
T9258	28393-28400	NNS	denotes	results
T9259	28401-28404	CC	denotes	and
T9260	28405-28408	DT	denotes	the
T9261	28409-28417	NNS	denotes	findings
T9262	28418-28426	VBN	denotes	obtained
T9263	28427-28431	IN	denotes	from
T9264	28432-28434	FW	denotes	in
T9265	28435-28440	FW	denotes	vitro
T9267	28441-28448	NN	denotes	culture
T9266	28449-28460	NNS	denotes	experiments
T9268	28461-28462	-LRB-	denotes	(
T9269	28462-28465	VB	denotes	see
T9270	28466-28473	NNS	denotes	Figures
T9271	28474-28475	CD	denotes	4
T9272	28476-28479	CC	denotes	and
T9273	28480-28481	CD	denotes	5
T9274	28481-28482	-RRB-	denotes	)
T9255	28483-28494	VBP	denotes	demonstrate
T9275	28495-28499	IN	denotes	that
T9277	28500-28510	JJ	denotes	precocious
T9279	28511-28513	CC	denotes	or
T9280	28514-28524	JJ	denotes	isochronic
T9278	28525-28535	NN	denotes	expression
T9281	28536-28538	IN	denotes	of
T9282	28539-28542	NN	denotes	EWS
T9284	28542-28543	HYPH	denotes	-
T9283	28543-28547	NN	denotes	Pea3
T9285	28548-28550	IN	denotes	in
T9286	28551-28554	DT	denotes	the
T9288	28555-28559	JJ	denotes	same
T9287	28560-28567	NNS	denotes	neurons
T9276	28568-28573	VBZ	denotes	leads
T9289	28574-28576	IN	denotes	to
T9290	28577-28590	RB	denotes	significantly
T9291	28591-28600	JJ	denotes	different
T9293	28601-28605	NN	denotes	cell
T9295	28605-28606	HYPH	denotes	-
T9294	28606-28616	JJ	denotes	autonomous
T9296	28617-28625	JJ	denotes	cellular
T9292	28626-28635	NNS	denotes	responses
T9297	28636-28640	IN	denotes	with
T9298	28641-28648	NN	denotes	respect
T9299	28649-28651	IN	denotes	to
T9300	28652-28656	NN	denotes	gene
T9301	28657-28667	NN	denotes	expression
T9302	28667-28669	,	denotes	,
T9303	28669-28677	JJ	denotes	neuronal
T9304	28678-28686	NN	denotes	survival
T9305	28686-28688	,	denotes	,
T9306	28688-28691	CC	denotes	and
T9307	28692-28699	NN	denotes	neurite
T9308	28700-28709	NN	denotes	outgrowth
T9309	28710-28711	-LRB-	denotes	(
T9310	28711-28717	NN	denotes	Figure
T9311	28718-28719	CD	denotes	9
T9312	28719-28720	-RRB-	denotes	)
T9313	28720-28721	.	denotes	.
T10766	31438-31444	NN	denotes	Target
T10768	31444-31445	HYPH	denotes	-
T10767	31445-31452	VBN	denotes	derived
T10769	31453-31460	NNS	denotes	signals
T10770	31461-31468	VBP	denotes	exhibit
T10771	31469-31470	DT	denotes	a
T10773	31471-31480	VBN	denotes	conserved
T10772	31481-31485	NN	denotes	role
T10774	31486-31488	IN	denotes	in
T10775	31489-31492	DT	denotes	the
T10776	31493-31502	NN	denotes	induction
T10777	31503-31505	IN	denotes	of
T10778	31506-31513	VBN	denotes	defined
T10779	31514-31522	NNS	denotes	programs
T10780	31523-31525	IN	denotes	of
T10781	31526-31539	NN	denotes	transcription
T10782	31540-31546	NN	denotes	factor
T10783	31547-31557	NN	denotes	expression
T10784	31558-31562	JJ	denotes	late
T10785	31563-31565	IN	denotes	in
T10786	31566-31578	JJ	denotes	post-mitotic
T10788	31579-31587	JJ	denotes	neuronal
T10787	31588-31603	NN	denotes	differentiation
T10789	31604-31605	-LRB-	denotes	[
T10790	31605-31607	CD	denotes	10
T10791	31607-31608	-RRB-	denotes	]
T10792	31608-31609	.	denotes	.
T10793	31609-31780	sentence	denotes	This study provides evidence that the late onset of transcription factor expression is essential for many later aspects of neuronal differentiation and circuit formation.
T10794	31610-31614	DT	denotes	This
T10795	31615-31620	NN	denotes	study
T10796	31621-31629	VBZ	denotes	provides
T10797	31630-31638	NN	denotes	evidence
T10798	31639-31643	IN	denotes	that
T10800	31644-31647	DT	denotes	the
T10802	31648-31652	JJ	denotes	late
T10801	31653-31658	NN	denotes	onset
T10803	31659-31661	IN	denotes	of
T10804	31662-31675	NN	denotes	transcription
T10805	31676-31682	NN	denotes	factor
T10806	31683-31693	NN	denotes	expression
T10799	31694-31696	VBZ	denotes	is
T10807	31697-31706	JJ	denotes	essential
T10808	31707-31710	IN	denotes	for
T10809	31711-31715	JJ	denotes	many
T10811	31716-31721	JJ	denotes	later
T10810	31722-31729	NNS	denotes	aspects
T10812	31730-31732	IN	denotes	of
T10813	31733-31741	JJ	denotes	neuronal
T10814	31742-31757	NN	denotes	differentiation
T10815	31758-31761	CC	denotes	and
T10816	31762-31769	NN	denotes	circuit
T10817	31770-31779	NN	denotes	formation
T10818	31779-31780	.	denotes	.
T10819	31780-31992	sentence	denotes	Our data indicate that DRG neurons undergo a temporal change in their competence to respond to ETS transcription factor signaling, as assessed by changes in gene expression and axonal target invasion (Figure 9).
T10820	31781-31784	PRP$	denotes	Our
T10821	31785-31789	NNS	denotes	data
T10822	31790-31798	VBP	denotes	indicate
T10823	31799-31803	IN	denotes	that
T10825	31804-31807	NN	denotes	DRG
T10826	31808-31815	NNS	denotes	neurons
T10824	31816-31823	VBP	denotes	undergo
T10827	31824-31825	DT	denotes	a
T10829	31826-31834	JJ	denotes	temporal
T10828	31835-31841	NN	denotes	change
T10830	31842-31844	IN	denotes	in
T10831	31845-31850	PRP$	denotes	their
T10832	31851-31861	NN	denotes	competence
T10833	31862-31864	TO	denotes	to
T10834	31865-31872	VB	denotes	respond
T10835	31873-31875	IN	denotes	to
T10836	31876-31879	NN	denotes	ETS
T10838	31880-31893	NN	denotes	transcription
T10839	31894-31900	NN	denotes	factor
T10837	31901-31910	NN	denotes	signaling
T10840	31910-31912	,	denotes	,
T10841	31912-31914	IN	denotes	as
T10842	31915-31923	VBN	denotes	assessed
T10843	31924-31926	IN	denotes	by
T10844	31927-31934	NNS	denotes	changes
T10845	31935-31937	IN	denotes	in
T10846	31938-31942	NN	denotes	gene
T10847	31943-31953	NN	denotes	expression
T10848	31954-31957	CC	denotes	and
T10849	31958-31964	JJ	denotes	axonal
T10851	31965-31971	NN	denotes	target
T10850	31972-31980	NN	denotes	invasion
T10852	31981-31982	-LRB-	denotes	(
T10853	31982-31988	NN	denotes	Figure
T10854	31989-31990	CD	denotes	9
T10855	31990-31991	-RRB-	denotes	)
T10856	31991-31992	.	denotes	.
T10857	31992-32137	sentence	denotes	Our findings argue for the necessity of target-induced, and therefore temporally controlled, upregulation of ETS transcription factor signaling.
T10858	31993-31996	PRP$	denotes	Our
T10859	31997-32005	NNS	denotes	findings
T10860	32006-32011	VBP	denotes	argue
T10861	32012-32015	IN	denotes	for
T10862	32016-32019	DT	denotes	the
T10863	32020-32029	NN	denotes	necessity
T10864	32030-32032	IN	denotes	of
T10865	32033-32039	NN	denotes	target
T10867	32039-32040	HYPH	denotes	-
T10866	32040-32047	VBN	denotes	induced
T10869	32047-32049	,	denotes	,
T10870	32049-32052	CC	denotes	and
T10871	32053-32062	RB	denotes	therefore
T10872	32063-32073	RB	denotes	temporally
T10873	32074-32084	VBN	denotes	controlled
T10874	32084-32086	,	denotes	,
T10868	32086-32098	NN	denotes	upregulation
T10875	32099-32101	IN	denotes	of
T10876	32102-32105	NN	denotes	ETS
T10878	32106-32119	NN	denotes	transcription
T10879	32120-32126	NN	denotes	factor
T10877	32127-32136	NN	denotes	signaling
T10880	32136-32137	.	denotes	.
T10881	32137-32367	sentence	denotes	More generally, they suggest that temporally regulated activation of transcriptional programs coupled to a particular fate induced in neurons at early developmental stages represents an important mechanism of neuronal maturation.
T10882	32138-32142	RBR	denotes	More
T10883	32143-32152	RB	denotes	generally
T10885	32152-32154	,	denotes	,
T10886	32154-32158	PRP	denotes	they
T10884	32159-32166	VBP	denotes	suggest
T10887	32167-32171	IN	denotes	that
T10889	32172-32182	RB	denotes	temporally
T10890	32183-32192	VBN	denotes	regulated
T10891	32193-32203	NN	denotes	activation
T10892	32204-32206	IN	denotes	of
T10893	32207-32222	JJ	denotes	transcriptional
T10894	32223-32231	NNS	denotes	programs
T10895	32232-32239	VBN	denotes	coupled
T10896	32240-32242	IN	denotes	to
T10897	32243-32244	DT	denotes	a
T10899	32245-32255	JJ	denotes	particular
T10898	32256-32260	NN	denotes	fate
T10900	32261-32268	VBN	denotes	induced
T10901	32269-32271	IN	denotes	in
T10902	32272-32279	NNS	denotes	neurons
T10903	32280-32282	IN	denotes	at
T10904	32283-32288	JJ	denotes	early
T10906	32289-32302	JJ	denotes	developmental
T10905	32303-32309	NNS	denotes	stages
T10888	32310-32320	VBZ	denotes	represents
T10907	32321-32323	DT	denotes	an
T10909	32324-32333	JJ	denotes	important
T10908	32334-32343	NN	denotes	mechanism
T10910	32344-32346	IN	denotes	of
T10911	32347-32355	JJ	denotes	neuronal
T10912	32356-32366	NN	denotes	maturation
T10913	32366-32367	.	denotes	.
T10914	32367-32578	sentence	denotes	One striking observation of this study is that precocious induction of ETS signaling promotes neuronal survival without a requirement for neurotrophic support and in complete absence of Trk receptor expression.
T10915	32368-32371	CD	denotes	One
T10917	32372-32380	JJ	denotes	striking
T10916	32381-32392	NN	denotes	observation
T10919	32393-32395	IN	denotes	of
T10920	32396-32400	DT	denotes	this
T10921	32401-32406	NN	denotes	study
T10918	32407-32409	VBZ	denotes	is
T10922	32410-32414	IN	denotes	that
T10924	32415-32425	JJ	denotes	precocious
T10925	32426-32435	NN	denotes	induction
T10926	32436-32438	IN	denotes	of
T10927	32439-32442	NN	denotes	ETS
T10928	32443-32452	NN	denotes	signaling
T10923	32453-32461	VBZ	denotes	promotes
T10929	32462-32470	JJ	denotes	neuronal
T10930	32471-32479	NN	denotes	survival
T10931	32480-32487	IN	denotes	without
T10932	32488-32489	DT	denotes	a
T10933	32490-32501	NN	denotes	requirement
T10934	32502-32505	IN	denotes	for
T10935	32506-32518	JJ	denotes	neurotrophic
T10936	32519-32526	NN	denotes	support
T10937	32527-32530	CC	denotes	and
T10938	32531-32533	IN	denotes	in
T10939	32534-32542	JJ	denotes	complete
T10940	32543-32550	NN	denotes	absence
T10941	32551-32553	IN	denotes	of
T10942	32554-32557	NN	denotes	Trk
T10943	32558-32566	NN	denotes	receptor
T10944	32567-32577	NN	denotes	expression
T10945	32577-32578	.	denotes	.
T10946	32578-32819	sentence	denotes	In contrast, ETS signaling at the normal time of onset of Er81 expression does not result in enhanced neuronal survival in the absence of neurotrophins and also does not lead to downregulation of TrkC expression in proprioceptive afferents.
T10947	32579-32581	IN	denotes	In
T10949	32582-32590	NN	denotes	contrast
T10950	32590-32592	,	denotes	,
T10951	32592-32595	NN	denotes	ETS
T10952	32596-32605	NN	denotes	signaling
T10953	32606-32608	IN	denotes	at
T10954	32609-32612	DT	denotes	the
T10956	32613-32619	JJ	denotes	normal
T10955	32620-32624	NN	denotes	time
T10957	32625-32627	IN	denotes	of
T10958	32628-32633	NN	denotes	onset
T10959	32634-32636	IN	denotes	of
T10960	32637-32641	NN	denotes	Er81
T10961	32642-32652	NN	denotes	expression
T10962	32653-32657	VBZ	denotes	does
T10963	32658-32661	RB	denotes	not
T10948	32662-32668	VB	denotes	result
T10964	32669-32671	IN	denotes	in
T10965	32672-32680	VBN	denotes	enhanced
T10967	32681-32689	JJ	denotes	neuronal
T10966	32690-32698	NN	denotes	survival
T10968	32699-32701	IN	denotes	in
T10969	32702-32705	DT	denotes	the
T10970	32706-32713	NN	denotes	absence
T10971	32714-32716	IN	denotes	of
T10972	32717-32730	NNS	denotes	neurotrophins
T10973	32731-32734	CC	denotes	and
T10974	32735-32739	RB	denotes	also
T10976	32740-32744	VBZ	denotes	does
T10977	32745-32748	RB	denotes	not
T10975	32749-32753	VB	denotes	lead
T10978	32754-32756	IN	denotes	to
T10979	32757-32771	NN	denotes	downregulation
T10980	32772-32774	IN	denotes	of
T10981	32775-32779	NN	denotes	TrkC
T10982	32780-32790	NN	denotes	expression
T10983	32791-32793	IN	denotes	in
T10984	32794-32808	JJ	denotes	proprioceptive
T10985	32809-32818	NNS	denotes	afferents
T10986	32818-32819	.	denotes	.
T10987	32819-32987	sentence	denotes	These findings demonstrate very distinct activities of one transcriptional regulator at different developmental steps within a committed post-mitotic neuronal lineage.
T10988	32820-32825	DT	denotes	These
T10989	32826-32834	NNS	denotes	findings
T10990	32835-32846	VBP	denotes	demonstrate
T10991	32847-32851	RB	denotes	very
T10992	32852-32860	JJ	denotes	distinct
T10993	32861-32871	NNS	denotes	activities
T10994	32872-32874	IN	denotes	of
T10995	32875-32878	CD	denotes	one
T10997	32879-32894	JJ	denotes	transcriptional
T10996	32895-32904	NN	denotes	regulator
T10998	32905-32907	IN	denotes	at
T10999	32908-32917	JJ	denotes	different
T11001	32918-32931	JJ	denotes	developmental
T11000	32932-32937	NNS	denotes	steps
T11002	32938-32944	IN	denotes	within
T11003	32945-32946	DT	denotes	a
T11005	32947-32956	VBN	denotes	committed
T11006	32957-32969	JJ	denotes	post-mitotic
T11007	32970-32978	JJ	denotes	neuronal
T11004	32979-32986	NN	denotes	lineage
T11008	32986-32987	.	denotes	.
T11009	32987-33142	sentence	denotes	The absence of Trk receptor expression upon precocious induction of ETS signaling can only partially explain the observed phenotype in axonal projections.
T11010	32988-32991	DT	denotes	The
T11011	32992-32999	NN	denotes	absence
T11013	33000-33002	IN	denotes	of
T11014	33003-33006	NN	denotes	Trk
T11016	33007-33015	NN	denotes	receptor
T11015	33016-33026	NN	denotes	expression
T11017	33027-33031	IN	denotes	upon
T11018	33032-33042	JJ	denotes	precocious
T11019	33043-33052	NN	denotes	induction
T11020	33053-33055	IN	denotes	of
T11021	33056-33059	NN	denotes	ETS
T11022	33060-33069	NN	denotes	signaling
T11023	33070-33073	MD	denotes	can
T11024	33074-33078	RB	denotes	only
T11025	33079-33088	RB	denotes	partially
T11012	33089-33096	VB	denotes	explain
T11026	33097-33100	DT	denotes	the
T11028	33101-33109	VBN	denotes	observed
T11027	33110-33119	NN	denotes	phenotype
T11029	33120-33122	IN	denotes	in
T11030	33123-33129	JJ	denotes	axonal
T11031	33130-33141	NNS	denotes	projections
T11032	33141-33142	.	denotes	.
T11033	33142-33354	sentence	denotes	Elimination of TrkA receptor signaling in Bax mutant mice perturbs establishment of peripheral projections of cutaneous afferents, whereas establishment of central projections does not appear to be affected [8].
T11034	33143-33154	NN	denotes	Elimination
T11036	33155-33157	IN	denotes	of
T11037	33158-33162	NN	denotes	TrkA
T11038	33163-33171	NN	denotes	receptor
T11039	33172-33181	NN	denotes	signaling
T11040	33182-33184	IN	denotes	in
T11041	33185-33188	NN	denotes	Bax
T11043	33189-33195	NN	denotes	mutant
T11042	33196-33200	NNS	denotes	mice
T11035	33201-33209	VBZ	denotes	perturbs
T11044	33210-33223	NN	denotes	establishment
T11045	33224-33226	IN	denotes	of
T11046	33227-33237	JJ	denotes	peripheral
T11047	33238-33249	NNS	denotes	projections
T11048	33250-33252	IN	denotes	of
T11049	33253-33262	JJ	denotes	cutaneous
T11050	33263-33272	NNS	denotes	afferents
T11051	33272-33274	,	denotes	,
T11052	33274-33281	IN	denotes	whereas
T11054	33282-33295	NN	denotes	establishment
T11055	33296-33298	IN	denotes	of
T11056	33299-33306	JJ	denotes	central
T11057	33307-33318	NNS	denotes	projections
T11058	33319-33323	VBZ	denotes	does
T11059	33324-33327	RB	denotes	not
T11053	33328-33334	VB	denotes	appear
T11060	33335-33337	TO	denotes	to
T11062	33338-33340	VB	denotes	be
T11061	33341-33349	VBN	denotes	affected
T11063	33350-33351	-LRB-	denotes	[
T11064	33351-33352	CD	denotes	8
T11065	33352-33353	-RRB-	denotes	]
T11066	33353-33354	.	denotes	.
T11067	33354-33487	sentence	denotes	In the absence of NT-3 signaling, development of central as well as peripheral proprioceptive afferent projections is perturbed [9].
T11068	33355-33357	IN	denotes	In
T11070	33358-33361	DT	denotes	the
T11071	33362-33369	NN	denotes	absence
T11072	33370-33372	IN	denotes	of
T11073	33373-33375	NN	denotes	NT
T11075	33375-33376	HYPH	denotes	-
T11076	33376-33377	CD	denotes	3
T11074	33378-33387	NN	denotes	signaling
T11077	33387-33389	,	denotes	,
T11078	33389-33400	NN	denotes	development
T11079	33401-33403	IN	denotes	of
T11080	33404-33411	JJ	denotes	central
T11082	33412-33414	RB	denotes	as
T11084	33415-33419	RB	denotes	well
T11083	33420-33422	IN	denotes	as
T11085	33423-33433	JJ	denotes	peripheral
T11086	33434-33448	JJ	denotes	proprioceptive
T11087	33449-33457	JJ	denotes	afferent
T11081	33458-33469	NNS	denotes	projections
T11088	33470-33472	VBZ	denotes	is
T11069	33473-33482	VBN	denotes	perturbed
T11089	33483-33484	-LRB-	denotes	[
T11090	33484-33485	CD	denotes	9
T11091	33485-33486	-RRB-	denotes	]
T11092	33486-33487	.	denotes	.
T11093	33487-33665	sentence	denotes	In contrast, upon precocious induction of ETS signaling, we found more pronounced defects in the establishment of central rather than peripheral projections for all DRG neurons.
T11094	33488-33490	IN	denotes	In
T11096	33491-33499	NN	denotes	contrast
T11097	33499-33501	,	denotes	,
T11098	33501-33505	IN	denotes	upon
T11099	33506-33516	JJ	denotes	precocious
T11100	33517-33526	NN	denotes	induction
T11101	33527-33529	IN	denotes	of
T11102	33530-33533	NN	denotes	ETS
T11103	33534-33543	NN	denotes	signaling
T11104	33543-33545	,	denotes	,
T11105	33545-33547	PRP	denotes	we
T11095	33548-33553	VBD	denotes	found
T11106	33554-33558	RBR	denotes	more
T11107	33559-33569	JJ	denotes	pronounced
T11108	33570-33577	NNS	denotes	defects
T11109	33578-33580	IN	denotes	in
T11110	33581-33584	DT	denotes	the
T11111	33585-33598	NN	denotes	establishment
T11112	33599-33601	IN	denotes	of
T11113	33602-33609	JJ	denotes	central
T11115	33610-33616	RB	denotes	rather
T11116	33617-33621	IN	denotes	than
T11117	33622-33632	JJ	denotes	peripheral
T11114	33633-33644	NNS	denotes	projections
T11118	33645-33648	IN	denotes	for
T11119	33649-33652	DT	denotes	all
T11121	33653-33656	NN	denotes	DRG
T11120	33657-33664	NNS	denotes	neurons
T11122	33664-33665	.	denotes	.
T11123	33665-33898	sentence	denotes	Induction of Er81 expression in proprioceptive afferents is controlled by peripheral NT-3 as axons reach the vicinity of target muscles, and thus occurs only approximately 3 d after proprioceptive neurons become post-mitotic [9,14].
T11124	33666-33675	NN	denotes	Induction
T11126	33676-33678	IN	denotes	of
T11127	33679-33683	NN	denotes	Er81
T11128	33684-33694	NN	denotes	expression
T11129	33695-33697	IN	denotes	in
T11130	33698-33712	JJ	denotes	proprioceptive
T11131	33713-33722	NNS	denotes	afferents
T11132	33723-33725	VBZ	denotes	is
T11125	33726-33736	VBN	denotes	controlled
T11133	33737-33739	IN	denotes	by
T11134	33740-33750	JJ	denotes	peripheral
T11135	33751-33753	NN	denotes	NT
T11136	33753-33754	HYPH	denotes	-
T11137	33754-33755	CD	denotes	3
T11138	33756-33758	IN	denotes	as
T11140	33759-33764	NNS	denotes	axons
T11139	33765-33770	VBP	denotes	reach
T11141	33771-33774	DT	denotes	the
T11142	33775-33783	NN	denotes	vicinity
T11143	33784-33786	IN	denotes	of
T11144	33787-33793	NN	denotes	target
T11145	33794-33801	NNS	denotes	muscles
T11146	33801-33803	,	denotes	,
T11147	33803-33806	CC	denotes	and
T11148	33807-33811	RB	denotes	thus
T11149	33812-33818	VBZ	denotes	occurs
T11150	33819-33823	RB	denotes	only
T11152	33824-33837	RB	denotes	approximately
T11153	33838-33839	CD	denotes	3
T11151	33840-33841	NN	denotes	d
T11155	33842-33847	IN	denotes	after
T11156	33848-33862	JJ	denotes	proprioceptive
T11157	33863-33870	NNS	denotes	neurons
T11154	33871-33877	VBP	denotes	become
T11158	33878-33890	JJ	denotes	post-mitotic
T11159	33891-33892	-LRB-	denotes	[
T11161	33892-33893	CD	denotes	9
T11162	33893-33894	,	denotes	,
T11160	33894-33896	CD	denotes	14
T11163	33896-33897	-RRB-	denotes	]
T11164	33897-33898	.	denotes	.
T11165	33898-34156	sentence	denotes	This temporally delayed and target-induced upregulation of ETS transcription factor expression several days after a neuronal lineage of a specific identity first emerges is not restricted to DRG sensory neurons, but is also found in motor neuron pools [13].
T11166	33899-33903	DT	denotes	This
T11168	33904-33914	RB	denotes	temporally
T11169	33915-33922	VBN	denotes	delayed
T11170	33923-33926	CC	denotes	and
T11171	33927-33933	NN	denotes	target
T11173	33933-33934	HYPH	denotes	-
T11172	33934-33941	VBN	denotes	induced
T11167	33942-33954	NN	denotes	upregulation
T11175	33955-33957	IN	denotes	of
T11176	33958-33961	NN	denotes	ETS
T11178	33962-33975	NN	denotes	transcription
T11179	33976-33982	NN	denotes	factor
T11177	33983-33993	NN	denotes	expression
T11180	33994-34001	JJ	denotes	several
T11181	34002-34006	NNS	denotes	days
T11183	34007-34012	IN	denotes	after
T11184	34013-34014	DT	denotes	a
T11186	34015-34023	JJ	denotes	neuronal
T11185	34024-34031	NN	denotes	lineage
T11187	34032-34034	IN	denotes	of
T11188	34035-34036	DT	denotes	a
T11190	34037-34045	JJ	denotes	specific
T11189	34046-34054	NN	denotes	identity
T11191	34055-34060	RB	denotes	first
T11182	34061-34068	VBZ	denotes	emerges
T11192	34069-34071	VBZ	denotes	is
T11193	34072-34075	RB	denotes	not
T11174	34076-34086	VBN	denotes	restricted
T11194	34087-34089	IN	denotes	to
T11195	34090-34093	NN	denotes	DRG
T11197	34094-34101	JJ	denotes	sensory
T11196	34102-34109	NNS	denotes	neurons
T11198	34109-34111	,	denotes	,
T11199	34111-34114	CC	denotes	but
T11200	34115-34117	VBZ	denotes	is
T11202	34118-34122	RB	denotes	also
T11201	34123-34128	VBN	denotes	found
T11203	34129-34131	IN	denotes	in
T11204	34132-34137	NN	denotes	motor
T11205	34138-34144	NN	denotes	neuron
T11206	34145-34150	NNS	denotes	pools
T11207	34151-34152	-LRB-	denotes	[
T11208	34152-34154	CD	denotes	13
T11209	34154-34155	-RRB-	denotes	]
T11210	34155-34156	.	denotes	.
T11211	34156-34543	sentence	denotes	Target-derived factors have also been implicated in controlling neuronal maturation of predetermined neurons in Drosophila, in which expression of members of the BMP family in the target region is essential for the induction of mature peptidergic properties in a subpopulation of neurons marked by the coordinate expression of the two transcription factors Apterous and Squeeze [11,12].
T11212	34157-34163	NN	denotes	Target
T11214	34163-34164	HYPH	denotes	-
T11213	34164-34171	VBN	denotes	derived
T11215	34172-34179	NNS	denotes	factors
T11217	34180-34184	VBP	denotes	have
T11218	34185-34189	RB	denotes	also
T11219	34190-34194	VBN	denotes	been
T11216	34195-34205	VBN	denotes	implicated
T11220	34206-34208	IN	denotes	in
T11221	34209-34220	VBG	denotes	controlling
T11222	34221-34229	JJ	denotes	neuronal
T11223	34230-34240	NN	denotes	maturation
T11224	34241-34243	IN	denotes	of
T11225	34244-34257	VBN	denotes	predetermined
T11226	34258-34265	NNS	denotes	neurons
T11227	34266-34268	IN	denotes	in
T11228	34269-34279	NNP	denotes	Drosophila
T11229	34279-34281	,	denotes	,
T11230	34281-34283	IN	denotes	in
T11232	34284-34289	WDT	denotes	which
T11233	34290-34300	NN	denotes	expression
T11234	34301-34303	IN	denotes	of
T11235	34304-34311	NNS	denotes	members
T11236	34312-34314	IN	denotes	of
T11237	34315-34318	DT	denotes	the
T11239	34319-34322	NN	denotes	BMP
T11238	34323-34329	NN	denotes	family
T11240	34330-34332	IN	denotes	in
T11241	34333-34336	DT	denotes	the
T11243	34337-34343	NN	denotes	target
T11242	34344-34350	NN	denotes	region
T11231	34351-34353	VBZ	denotes	is
T11244	34354-34363	JJ	denotes	essential
T11245	34364-34367	IN	denotes	for
T11246	34368-34371	DT	denotes	the
T11247	34372-34381	NN	denotes	induction
T11248	34382-34384	IN	denotes	of
T11249	34385-34391	JJ	denotes	mature
T11251	34392-34403	JJ	denotes	peptidergic
T11250	34404-34414	NNS	denotes	properties
T11252	34415-34417	IN	denotes	in
T11253	34418-34419	DT	denotes	a
T11254	34420-34433	NN	denotes	subpopulation
T11255	34434-34436	IN	denotes	of
T11256	34437-34444	NNS	denotes	neurons
T11257	34445-34451	VBN	denotes	marked
T11258	34452-34454	IN	denotes	by
T11259	34455-34458	DT	denotes	the
T11261	34459-34469	NN	denotes	coordinate
T11260	34470-34480	NN	denotes	expression
T11262	34481-34483	IN	denotes	of
T11263	34484-34487	DT	denotes	the
T11265	34488-34491	CD	denotes	two
T11266	34492-34505	NN	denotes	transcription
T11264	34506-34513	NNS	denotes	factors
T11267	34514-34522	NN	denotes	Apterous
T11268	34523-34526	CC	denotes	and
T11269	34527-34534	NN	denotes	Squeeze
T11270	34535-34536	-LRB-	denotes	[
T11272	34536-34538	CD	denotes	11
T11273	34538-34539	,	denotes	,
T11271	34539-34541	CD	denotes	12
T11274	34541-34542	-RRB-	denotes	]
T11275	34542-34543	.	denotes	.
T11276	34543-34730	sentence	denotes	Thus, both in Drosophila and vertebrates, target-derived factors appear to act permissively to induce the expression of transcriptional programs involved in terminal neuronal maturation.
T11277	34544-34548	RB	denotes	Thus
T11279	34548-34550	,	denotes	,
T11280	34550-34554	CC	denotes	both
T11281	34555-34557	IN	denotes	in
T11282	34558-34568	NNP	denotes	Drosophila
T11283	34569-34572	CC	denotes	and
T11284	34573-34584	NNS	denotes	vertebrates
T11285	34584-34586	,	denotes	,
T11286	34586-34592	NN	denotes	target
T11288	34592-34593	HYPH	denotes	-
T11287	34593-34600	VBN	denotes	derived
T11289	34601-34608	NNS	denotes	factors
T11278	34609-34615	VBP	denotes	appear
T11290	34616-34618	TO	denotes	to
T11291	34619-34622	VB	denotes	act
T11292	34623-34635	RB	denotes	permissively
T11293	34636-34638	TO	denotes	to
T11294	34639-34645	VB	denotes	induce
T11295	34646-34649	DT	denotes	the
T11296	34650-34660	NN	denotes	expression
T11297	34661-34663	IN	denotes	of
T11298	34664-34679	JJ	denotes	transcriptional
T11299	34680-34688	NNS	denotes	programs
T11300	34689-34697	VBN	denotes	involved
T11301	34698-34700	IN	denotes	in
T11302	34701-34709	JJ	denotes	terminal
T11304	34710-34718	JJ	denotes	neuronal
T11303	34719-34729	NN	denotes	maturation
T11305	34729-34730	.	denotes	.
T11306	34730-34908	sentence	denotes	Our findings are compatible with a model in which DRG neurons acquire their mature fate by sequential and temporally controlled addition of lineage-specific features (Figure 9).
T11307	34731-34734	PRP$	denotes	Our
T11308	34735-34743	NNS	denotes	findings
T11309	34744-34747	VBP	denotes	are
T11310	34748-34758	JJ	denotes	compatible
T11311	34759-34763	IN	denotes	with
T11312	34764-34765	DT	denotes	a
T11313	34766-34771	NN	denotes	model
T11314	34772-34774	IN	denotes	in
T11316	34775-34780	WDT	denotes	which
T11317	34781-34784	NN	denotes	DRG
T11318	34785-34792	NNS	denotes	neurons
T11315	34793-34800	VBP	denotes	acquire
T11319	34801-34806	PRP$	denotes	their
T11321	34807-34813	JJ	denotes	mature
T11320	34814-34818	NN	denotes	fate
T11322	34819-34821	IN	denotes	by
T11323	34822-34832	JJ	denotes	sequential
T11325	34833-34836	CC	denotes	and
T11326	34837-34847	RB	denotes	temporally
T11327	34848-34858	VBN	denotes	controlled
T11324	34859-34867	NN	denotes	addition
T11328	34868-34870	IN	denotes	of
T11329	34871-34878	NN	denotes	lineage
T11331	34878-34879	HYPH	denotes	-
T11330	34879-34887	JJ	denotes	specific
T11332	34888-34896	NNS	denotes	features
T11333	34897-34898	-LRB-	denotes	(
T11334	34898-34904	NN	denotes	Figure
T11335	34905-34906	CD	denotes	9
T11336	34906-34907	-RRB-	denotes	)
T11337	34907-34908	.	denotes	.
T11338	34908-35084	sentence	denotes	Target-derived factors act on predetermined neuronal lineages to switch their developmental programs to become compatible with processes such as target invasion and branching.
T11339	34909-34915	NN	denotes	Target
T11341	34915-34916	HYPH	denotes	-
T11340	34916-34923	VBN	denotes	derived
T11342	34924-34931	NNS	denotes	factors
T11343	34932-34935	VBP	denotes	act
T11344	34936-34938	IN	denotes	on
T11345	34939-34952	VBN	denotes	predetermined
T11347	34953-34961	JJ	denotes	neuronal
T11346	34962-34970	NNS	denotes	lineages
T11348	34971-34973	TO	denotes	to
T11349	34974-34980	VB	denotes	switch
T11350	34981-34986	PRP$	denotes	their
T11352	34987-35000	JJ	denotes	developmental
T11351	35001-35009	NNS	denotes	programs
T11353	35010-35012	TO	denotes	to
T11354	35013-35019	VB	denotes	become
T11355	35020-35030	JJ	denotes	compatible
T11356	35031-35035	IN	denotes	with
T11357	35036-35045	NNS	denotes	processes
T11358	35046-35050	JJ	denotes	such
T11359	35051-35053	IN	denotes	as
T11360	35054-35060	NN	denotes	target
T11361	35061-35069	NN	denotes	invasion
T11362	35070-35073	CC	denotes	and
T11363	35074-35083	NN	denotes	branching
T11364	35083-35084	.	denotes	.
T11365	35084-35290	sentence	denotes	Such a transition state in the acquisition of a defined neuronal fate would be accompanied by the induction of appropriate transcriptional programs through the expression of specific transcription factors.
T11366	35085-35089	PDT	denotes	Such
T11368	35090-35091	DT	denotes	a
T11369	35092-35102	NN	denotes	transition
T11367	35103-35108	NN	denotes	state
T11371	35109-35111	IN	denotes	in
T11372	35112-35115	DT	denotes	the
T11373	35116-35127	NN	denotes	acquisition
T11374	35128-35130	IN	denotes	of
T11375	35131-35132	DT	denotes	a
T11377	35133-35140	VBN	denotes	defined
T11378	35141-35149	JJ	denotes	neuronal
T11376	35150-35154	NN	denotes	fate
T11379	35155-35160	MD	denotes	would
T11380	35161-35163	VB	denotes	be
T11370	35164-35175	VBN	denotes	accompanied
T11381	35176-35178	IN	denotes	by
T11382	35179-35182	DT	denotes	the
T11383	35183-35192	NN	denotes	induction
T11384	35193-35195	IN	denotes	of
T11385	35196-35207	JJ	denotes	appropriate
T11387	35208-35223	JJ	denotes	transcriptional
T11386	35224-35232	NNS	denotes	programs
T11388	35233-35240	IN	denotes	through
T11389	35241-35244	DT	denotes	the
T11390	35245-35255	NN	denotes	expression
T11391	35256-35258	IN	denotes	of
T11392	35259-35267	JJ	denotes	specific
T11394	35268-35281	NN	denotes	transcription
T11393	35282-35289	NNS	denotes	factors
T11395	35289-35290	.	denotes	.
T11396	35290-35694	sentence	denotes	Mechanisms such as chromosomal remodeling that restrict or expand access to certain target genes [34] or activation by cofactors responsible for changing the action of particular transcription factors [35] could represent possible mechanisms by which the downstream transcriptional profile of a transcription factor could be temporally shifted towards the selection and control of distinct target genes.
T11397	35291-35301	NNS	denotes	Mechanisms
T11399	35302-35306	JJ	denotes	such
T11400	35307-35309	IN	denotes	as
T11401	35310-35321	JJ	denotes	chromosomal
T11402	35322-35332	NN	denotes	remodeling
T11403	35333-35337	WDT	denotes	that
T11404	35338-35346	VB	denotes	restrict
T11405	35347-35349	CC	denotes	or
T11406	35350-35356	VB	denotes	expand
T11407	35357-35363	NN	denotes	access
T11408	35364-35366	IN	denotes	to
T11409	35367-35374	JJ	denotes	certain
T11411	35375-35381	NN	denotes	target
T11410	35382-35387	NNS	denotes	genes
T11412	35388-35389	-LRB-	denotes	[
T11413	35389-35391	CD	denotes	34
T11414	35391-35392	-RRB-	denotes	]
T11415	35393-35395	CC	denotes	or
T11416	35396-35406	NN	denotes	activation
T11417	35407-35409	IN	denotes	by
T11418	35410-35419	NNS	denotes	cofactors
T11419	35420-35431	JJ	denotes	responsible
T11420	35432-35435	IN	denotes	for
T11421	35436-35444	VBG	denotes	changing
T11422	35445-35448	DT	denotes	the
T11423	35449-35455	NN	denotes	action
T11424	35456-35458	IN	denotes	of
T11425	35459-35469	JJ	denotes	particular
T11427	35470-35483	NN	denotes	transcription
T11426	35484-35491	NNS	denotes	factors
T11428	35492-35493	-LRB-	denotes	[
T11429	35493-35495	CD	denotes	35
T11430	35495-35496	-RRB-	denotes	]
T11431	35497-35502	MD	denotes	could
T11398	35503-35512	VB	denotes	represent
T11432	35513-35521	JJ	denotes	possible
T11433	35522-35532	NNS	denotes	mechanisms
T11434	35533-35535	IN	denotes	by
T11436	35536-35541	WDT	denotes	which
T11437	35542-35545	DT	denotes	the
T11439	35546-35556	JJ	denotes	downstream
T11440	35557-35572	JJ	denotes	transcriptional
T11438	35573-35580	NN	denotes	profile
T11441	35581-35583	IN	denotes	of
T11442	35584-35585	DT	denotes	a
T11444	35586-35599	NN	denotes	transcription
T11443	35600-35606	NN	denotes	factor
T11445	35607-35612	MD	denotes	could
T11446	35613-35615	VB	denotes	be
T11447	35616-35626	RB	denotes	temporally
T11435	35627-35634	VBN	denotes	shifted
T11448	35635-35642	IN	denotes	towards
T11449	35643-35646	DT	denotes	the
T11450	35647-35656	NN	denotes	selection
T11451	35657-35660	CC	denotes	and
T11452	35661-35668	NN	denotes	control
T11453	35669-35671	IN	denotes	of
T11454	35672-35680	JJ	denotes	distinct
T11456	35681-35687	NN	denotes	target
T11455	35688-35693	NNS	denotes	genes
T11457	35693-35694	.	denotes	.
T11458	35694-35878	sentence	denotes	Interestingly, several ETS transcription factors are activated through release of autoinhibition via interaction with cofactors and/or via post-translational modifications [35,36,37].
T11459	35695-35708	RB	denotes	Interestingly
T11461	35708-35710	,	denotes	,
T11462	35710-35717	JJ	denotes	several
T11464	35718-35721	NN	denotes	ETS
T11465	35722-35735	NN	denotes	transcription
T11463	35736-35743	NNS	denotes	factors
T11466	35744-35747	VBP	denotes	are
T11460	35748-35757	VBN	denotes	activated
T11467	35758-35765	IN	denotes	through
T11468	35766-35773	NN	denotes	release
T11469	35774-35776	IN	denotes	of
T11470	35777-35791	NN	denotes	autoinhibition
T11471	35792-35795	IN	denotes	via
T11472	35796-35807	NN	denotes	interaction
T11473	35808-35812	IN	denotes	with
T11474	35813-35822	NNS	denotes	cofactors
T11475	35823-35826	CC	denotes	and
T11476	35826-35827	HYPH	denotes	/
T11477	35827-35829	CC	denotes	or
T11478	35830-35833	IN	denotes	via
T11479	35834-35852	JJ	denotes	post-translational
T11480	35853-35866	NNS	denotes	modifications
T11481	35867-35868	-LRB-	denotes	[
T11483	35868-35870	CD	denotes	35
T11484	35870-35871	,	denotes	,
T11485	35871-35873	CD	denotes	36
T11486	35873-35874	,	denotes	,
T11482	35874-35876	CD	denotes	37
T11487	35876-35877	-RRB-	denotes	]
T11488	35877-35878	.	denotes	.
T11489	35878-36123	sentence	denotes	The fusion of EWS with Pea3 could circumvent a need for activation through specific cofactors while still maintaining ETS site dependence, thus rendering EWS-Pea3 less sensitive to the cellular context than endogenous ETS transcription factors.
T11490	35879-35882	DT	denotes	The
T11491	35883-35889	NN	denotes	fusion
T11493	35890-35892	IN	denotes	of
T11494	35893-35896	NN	denotes	EWS
T11495	35897-35901	IN	denotes	with
T11496	35902-35906	NN	denotes	Pea3
T11497	35907-35912	MD	denotes	could
T11492	35913-35923	VB	denotes	circumvent
T11498	35924-35925	DT	denotes	a
T11499	35926-35930	NN	denotes	need
T11500	35931-35934	IN	denotes	for
T11501	35935-35945	NN	denotes	activation
T11502	35946-35953	IN	denotes	through
T11503	35954-35962	JJ	denotes	specific
T11504	35963-35972	NNS	denotes	cofactors
T11505	35973-35978	IN	denotes	while
T11507	35979-35984	RB	denotes	still
T11506	35985-35996	VBG	denotes	maintaining
T11508	35997-36000	NN	denotes	ETS
T11510	36001-36005	NN	denotes	site
T11509	36006-36016	NN	denotes	dependence
T11511	36016-36018	,	denotes	,
T11512	36018-36022	RB	denotes	thus
T11513	36023-36032	VBG	denotes	rendering
T11514	36033-36036	NN	denotes	EWS
T11516	36036-36037	HYPH	denotes	-
T11515	36037-36041	NN	denotes	Pea3
T11517	36042-36046	RBR	denotes	less
T11518	36047-36056	JJ	denotes	sensitive
T11519	36057-36059	IN	denotes	to
T11520	36060-36063	DT	denotes	the
T11522	36064-36072	JJ	denotes	cellular
T11521	36073-36080	NN	denotes	context
T11523	36081-36085	IN	denotes	than
T11524	36086-36096	JJ	denotes	endogenous
T11526	36097-36100	NN	denotes	ETS
T11527	36101-36114	NN	denotes	transcription
T11525	36115-36122	NNS	denotes	factors
T11528	36122-36123	.	denotes	.
T11529	36123-36313	sentence	denotes	Using this fusion protein, our experiments demonstrate a profound change in the action of ETS signaling at the level of transcriptional regulation within post-mitotic DRG neurons over time.
T11530	36124-36129	VBG	denotes	Using
T11532	36130-36134	DT	denotes	this
T11534	36135-36141	NN	denotes	fusion
T11533	36142-36149	NN	denotes	protein
T11535	36149-36151	,	denotes	,
T11536	36151-36154	PRP$	denotes	our
T11537	36155-36166	NNS	denotes	experiments
T11531	36167-36178	VBP	denotes	demonstrate
T11538	36179-36180	DT	denotes	a
T11540	36181-36189	JJ	denotes	profound
T11539	36190-36196	NN	denotes	change
T11541	36197-36199	IN	denotes	in
T11542	36200-36203	DT	denotes	the
T11543	36204-36210	NN	denotes	action
T11544	36211-36213	IN	denotes	of
T11545	36214-36217	NN	denotes	ETS
T11546	36218-36227	NN	denotes	signaling
T11547	36228-36230	IN	denotes	at
T11548	36231-36234	DT	denotes	the
T11549	36235-36240	NN	denotes	level
T11550	36241-36243	IN	denotes	of
T11551	36244-36259	JJ	denotes	transcriptional
T11552	36260-36270	NN	denotes	regulation
T11553	36271-36277	IN	denotes	within
T11554	36278-36290	JJ	denotes	post-mitotic
T11556	36291-36294	NN	denotes	DRG
T11555	36295-36302	NNS	denotes	neurons
T11557	36303-36307	IN	denotes	over
T11558	36308-36312	NN	denotes	time
T11559	36312-36313	.	denotes	.
T11560	36313-36522	sentence	denotes	Moreover, the observed transcriptional shift in ETS signaling is paired with the onset of appropriate regulation of neuronal subtype specification and establishment of axonal projections into the target area.
T11561	36314-36322	RB	denotes	Moreover
T11563	36322-36324	,	denotes	,
T11564	36324-36327	DT	denotes	the
T11566	36328-36336	VBN	denotes	observed
T11567	36337-36352	JJ	denotes	transcriptional
T11565	36353-36358	NN	denotes	shift
T11568	36359-36361	IN	denotes	in
T11569	36362-36365	NN	denotes	ETS
T11570	36366-36375	NN	denotes	signaling
T11571	36376-36378	VBZ	denotes	is
T11562	36379-36385	VBN	denotes	paired
T11572	36386-36390	IN	denotes	with
T11573	36391-36394	DT	denotes	the
T11574	36395-36400	NN	denotes	onset
T11575	36401-36403	IN	denotes	of
T11576	36404-36415	JJ	denotes	appropriate
T11577	36416-36426	NN	denotes	regulation
T11578	36427-36429	IN	denotes	of
T11579	36430-36438	JJ	denotes	neuronal
T11581	36439-36446	NN	denotes	subtype
T11580	36447-36460	NN	denotes	specification
T11582	36461-36464	CC	denotes	and
T11583	36465-36478	NN	denotes	establishment
T11584	36479-36481	IN	denotes	of
T11585	36482-36488	JJ	denotes	axonal
T11586	36489-36500	NNS	denotes	projections
T11587	36501-36505	IN	denotes	into
T11588	36506-36509	DT	denotes	the
T11590	36510-36516	NN	denotes	target
T11589	36517-36521	NN	denotes	area
T11591	36521-36522	.	denotes	.
T11592	36522-36833	sentence	denotes	Recent experiments addressing the temporal constraints of transcription factor action in proliferating neural progenitor cells adds to the idea that defined temporal windows, during which transcription factors act to control distinct downstream target genes, are of key importance to neuronal fate acquisition.
T11593	36523-36529	JJ	denotes	Recent
T11594	36530-36541	NNS	denotes	experiments
T11596	36542-36552	VBG	denotes	addressing
T11597	36553-36556	DT	denotes	the
T11599	36557-36565	JJ	denotes	temporal
T11598	36566-36577	NNS	denotes	constraints
T11600	36578-36580	IN	denotes	of
T11601	36581-36594	NN	denotes	transcription
T11602	36595-36601	NN	denotes	factor
T11603	36602-36608	NN	denotes	action
T11604	36609-36611	IN	denotes	in
T11605	36612-36625	VBG	denotes	proliferating
T11607	36626-36632	JJ	denotes	neural
T11608	36633-36643	NN	denotes	progenitor
T11606	36644-36649	NNS	denotes	cells
T11595	36650-36654	VBZ	denotes	adds
T11609	36655-36657	IN	denotes	to
T11610	36658-36661	DT	denotes	the
T11611	36662-36666	NN	denotes	idea
T11612	36667-36671	IN	denotes	that
T11614	36672-36679	VBN	denotes	defined
T11616	36680-36688	JJ	denotes	temporal
T11615	36689-36696	NNS	denotes	windows
T11617	36696-36698	,	denotes	,
T11618	36698-36704	IN	denotes	during
T11620	36705-36710	WDT	denotes	which
T11621	36711-36724	NN	denotes	transcription
T11622	36725-36732	NNS	denotes	factors
T11619	36733-36736	VBP	denotes	act
T11623	36737-36739	TO	denotes	to
T11624	36740-36747	VB	denotes	control
T11625	36748-36756	JJ	denotes	distinct
T11627	36757-36767	JJ	denotes	downstream
T11628	36768-36774	NN	denotes	target
T11626	36775-36780	NNS	denotes	genes
T11629	36780-36782	,	denotes	,
T11613	36782-36785	VBP	denotes	are
T11630	36786-36788	IN	denotes	of
T11631	36789-36792	JJ	denotes	key
T11632	36793-36803	NN	denotes	importance
T11633	36804-36806	IN	denotes	to
T11634	36807-36815	JJ	denotes	neuronal
T11636	36816-36820	NN	denotes	fate
T11635	36821-36832	NN	denotes	acquisition
T11637	36832-36833	.	denotes	.
T11638	36833-36984	sentence	denotes	During Drosophila neuroblast generation, the transcription factor Hunchback controls specification and differentiation of early-born neuroblasts [38].
T11639	36834-36840	IN	denotes	During
T11641	36841-36851	NNP	denotes	Drosophila
T11643	36852-36862	NN	denotes	neuroblast
T11642	36863-36873	NN	denotes	generation
T11644	36873-36875	,	denotes	,
T11645	36875-36878	DT	denotes	the
T11647	36879-36892	NN	denotes	transcription
T11648	36893-36899	NN	denotes	factor
T11646	36900-36909	NN	denotes	Hunchback
T11640	36910-36918	VBZ	denotes	controls
T11649	36919-36932	NN	denotes	specification
T11650	36933-36936	CC	denotes	and
T11651	36937-36952	NN	denotes	differentiation
T11652	36953-36955	IN	denotes	of
T11653	36956-36961	RB	denotes	early
T11655	36961-36962	HYPH	denotes	-
T11654	36962-36966	VBN	denotes	born
T11656	36967-36978	NNS	denotes	neuroblasts
T11657	36979-36980	-LRB-	denotes	[
T11658	36980-36982	CD	denotes	38
T11659	36982-36983	-RRB-	denotes	]
T11660	36983-36984	.	denotes	.
T11661	36984-37129	sentence	denotes	Over time, however, neuroblasts progressively lose their competence to generate cells of an early fate in response to Hunchback expression [39].
T11662	36985-36989	IN	denotes	Over
T11664	36990-36994	NN	denotes	time
T11665	36994-36996	,	denotes	,
T11666	36996-37003	RB	denotes	however
T11667	37003-37005	,	denotes	,
T11668	37005-37016	NNS	denotes	neuroblasts
T11669	37017-37030	RB	denotes	progressively
T11663	37031-37035	VBP	denotes	lose
T11670	37036-37041	PRP$	denotes	their
T11671	37042-37052	NN	denotes	competence
T11672	37053-37055	TO	denotes	to
T11673	37056-37064	VB	denotes	generate
T11674	37065-37070	NNS	denotes	cells
T11675	37071-37073	IN	denotes	of
T11676	37074-37076	DT	denotes	an
T11678	37077-37082	JJ	denotes	early
T11677	37083-37087	NN	denotes	fate
T11679	37088-37090	IN	denotes	in
T11680	37091-37099	NN	denotes	response
T11681	37100-37102	IN	denotes	to
T11682	37103-37112	NN	denotes	Hunchback
T11683	37113-37123	NN	denotes	expression
T11684	37124-37125	-LRB-	denotes	[
T11685	37125-37127	CD	denotes	39
T11686	37127-37128	-RRB-	denotes	]
T11687	37128-37129	.	denotes	.
T11688	37129-37290	sentence	denotes	These findings thus also argue for a change in cellular competence to respond to a specific transcription factor over time albeit in an early precursor context.
T11689	37130-37135	DT	denotes	These
T11690	37136-37144	NNS	denotes	findings
T11692	37145-37149	RB	denotes	thus
T11693	37150-37154	RB	denotes	also
T11691	37155-37160	VBP	denotes	argue
T11694	37161-37164	IN	denotes	for
T11695	37165-37166	DT	denotes	a
T11696	37167-37173	NN	denotes	change
T11697	37174-37176	IN	denotes	in
T11698	37177-37185	JJ	denotes	cellular
T11699	37186-37196	NN	denotes	competence
T11700	37197-37199	TO	denotes	to
T11701	37200-37207	VB	denotes	respond
T11702	37208-37210	IN	denotes	to
T11703	37211-37212	DT	denotes	a
T11705	37213-37221	JJ	denotes	specific
T11706	37222-37235	NN	denotes	transcription
T11704	37236-37242	NN	denotes	factor
T11707	37243-37247	IN	denotes	over
T11708	37248-37252	NN	denotes	time
T11709	37253-37259	IN	denotes	albeit
T11710	37260-37262	IN	denotes	in
T11711	37263-37265	DT	denotes	an
T11713	37266-37271	JJ	denotes	early
T11714	37272-37281	NN	denotes	precursor
T11712	37282-37289	NN	denotes	context
T11715	37289-37290	.	denotes	.
T11716	37290-37493	sentence	denotes	More generally, during the differentiation of hematopoietic lineages, several transcription factors have also been shown to exhibit distinct functions at progressive steps of lineage specification [40].
T11717	37291-37295	RBR	denotes	More
T11718	37296-37305	RB	denotes	generally
T11720	37305-37307	,	denotes	,
T11721	37307-37313	IN	denotes	during
T11722	37314-37317	DT	denotes	the
T11723	37318-37333	NN	denotes	differentiation
T11724	37334-37336	IN	denotes	of
T11725	37337-37350	JJ	denotes	hematopoietic
T11726	37351-37359	NNS	denotes	lineages
T11727	37359-37361	,	denotes	,
T11728	37361-37368	JJ	denotes	several
T11730	37369-37382	NN	denotes	transcription
T11729	37383-37390	NNS	denotes	factors
T11731	37391-37395	VBP	denotes	have
T11732	37396-37400	RB	denotes	also
T11733	37401-37405	VBN	denotes	been
T11719	37406-37411	VBN	denotes	shown
T11734	37412-37414	TO	denotes	to
T11735	37415-37422	VB	denotes	exhibit
T11736	37423-37431	JJ	denotes	distinct
T11737	37432-37441	NNS	denotes	functions
T11738	37442-37444	IN	denotes	at
T11739	37445-37456	JJ	denotes	progressive
T11740	37457-37462	NNS	denotes	steps
T11741	37463-37465	IN	denotes	of
T11742	37466-37473	NN	denotes	lineage
T11743	37474-37487	NN	denotes	specification
T11744	37488-37489	-LRB-	denotes	[
T11745	37489-37491	CD	denotes	40
T11746	37491-37492	-RRB-	denotes	]
T11747	37492-37493	.	denotes	.
T11748	37493-37861	sentence	denotes	Analysis of the mechanisms by which transcription factor programs can be shifted over time to control different complements of downstream genes and thus different aspects of neuronal and cellular fates in progenitor cells or post-mitotic neurons may provide further insight into the way in which transcription factors act to control the assembly of neuronal circuits.
T11749	37494-37502	NN	denotes	Analysis
T11751	37503-37505	IN	denotes	of
T11752	37506-37509	DT	denotes	the
T11753	37510-37520	NNS	denotes	mechanisms
T11754	37521-37523	IN	denotes	by
T11756	37524-37529	WDT	denotes	which
T11757	37530-37543	NN	denotes	transcription
T11758	37544-37550	NN	denotes	factor
T11759	37551-37559	NNS	denotes	programs
T11760	37560-37563	MD	denotes	can
T11761	37564-37566	VB	denotes	be
T11755	37567-37574	VBN	denotes	shifted
T11762	37575-37579	IN	denotes	over
T11763	37580-37584	NN	denotes	time
T11764	37585-37587	TO	denotes	to
T11765	37588-37595	VB	denotes	control
T11766	37596-37605	JJ	denotes	different
T11767	37606-37617	NNS	denotes	complements
T11768	37618-37620	IN	denotes	of
T11769	37621-37631	JJ	denotes	downstream
T11770	37632-37637	NNS	denotes	genes
T11771	37638-37641	CC	denotes	and
T11772	37642-37646	RB	denotes	thus
T11773	37647-37656	JJ	denotes	different
T11774	37657-37664	NNS	denotes	aspects
T11775	37665-37667	IN	denotes	of
T11776	37668-37676	JJ	denotes	neuronal
T11778	37677-37680	CC	denotes	and
T11779	37681-37689	JJ	denotes	cellular
T11777	37690-37695	NNS	denotes	fates
T11780	37696-37698	IN	denotes	in
T11781	37699-37709	NN	denotes	progenitor
T11782	37710-37715	NNS	denotes	cells
T11783	37716-37718	CC	denotes	or
T11784	37719-37731	JJ	denotes	post-mitotic
T11785	37732-37739	NNS	denotes	neurons
T11786	37740-37743	MD	denotes	may
T11750	37744-37751	VB	denotes	provide
T11787	37752-37759	JJ	denotes	further
T11788	37760-37767	NN	denotes	insight
T11789	37768-37772	IN	denotes	into
T11790	37773-37776	DT	denotes	the
T11791	37777-37780	NN	denotes	way
T11792	37781-37783	IN	denotes	in
T11794	37784-37789	WDT	denotes	which
T11795	37790-37803	NN	denotes	transcription
T11796	37804-37811	NNS	denotes	factors
T11793	37812-37815	VBP	denotes	act
T11797	37816-37818	TO	denotes	to
T11798	37819-37826	VB	denotes	control
T11799	37827-37830	DT	denotes	the
T11800	37831-37839	NN	denotes	assembly
T11801	37840-37842	IN	denotes	of
T11802	37843-37851	JJ	denotes	neuronal
T11803	37852-37860	NNS	denotes	circuits
T11804	37860-37861	.	denotes	.
T12345	37886-37896	NN	denotes	Generation
T12346	37897-37899	IN	denotes	of
T12347	37900-37910	JJ	denotes	transgenic
T12348	37911-37915	NNS	denotes	mice
T12349	37916-37919	CC	denotes	and
T12350	37920-37925	NN	denotes	mouse
T12351	37926-37934	NN	denotes	genetics
T12352	37934-38055	sentence	denotes	Er81EWS-Pea3 mice were generated following a strategy similar to that described for the generation of Er81NLZ mice [14].
T12353	37935-37942	NN	denotes	Er81EWS
T12355	37942-37943	HYPH	denotes	-
T12354	37943-37947	NN	denotes	Pea3
T12356	37948-37952	NNS	denotes	mice
T12358	37953-37957	VBD	denotes	were
T12357	37958-37967	VBN	denotes	generated
T12359	37968-37977	VBG	denotes	following
T12360	37978-37979	DT	denotes	a
T12361	37980-37988	NN	denotes	strategy
T12362	37989-37996	JJ	denotes	similar
T12363	37997-37999	IN	denotes	to
T12364	38000-38004	DT	denotes	that
T12365	38005-38014	VBN	denotes	described
T12366	38015-38018	IN	denotes	for
T12367	38019-38022	DT	denotes	the
T12368	38023-38033	NN	denotes	generation
T12369	38034-38036	IN	denotes	of
T12370	38037-38044	NN	denotes	Er81NLZ
T12371	38045-38049	NNS	denotes	mice
T12372	38050-38051	-LRB-	denotes	[
T12373	38051-38053	CD	denotes	14
T12374	38053-38054	-RRB-	denotes	]
T12375	38054-38055	.	denotes	.
T12376	38055-38261	sentence	denotes	In brief, a targeting vector with a cDNA coding for EWS-Pea3 was inserted in frame with the endogenous start ATG into exon 2 of the Er81 genomic locus and used for homologous recombination in W95 ES cells.
T12377	38056-38058	IN	denotes	In
T12379	38059-38064	NN	denotes	brief
T12380	38064-38066	,	denotes	,
T12381	38066-38067	DT	denotes	a
T12383	38068-38077	NN	denotes	targeting
T12382	38078-38084	NN	denotes	vector
T12384	38085-38089	IN	denotes	with
T12385	38090-38091	DT	denotes	a
T12387	38092-38096	NN	denotes	cDNA
T12386	38097-38103	NN	denotes	coding
T12388	38104-38107	IN	denotes	for
T12389	38108-38111	NN	denotes	EWS
T12391	38111-38112	HYPH	denotes	-
T12390	38112-38116	NN	denotes	Pea3
T12392	38117-38120	VBD	denotes	was
T12378	38121-38129	VBN	denotes	inserted
T12393	38130-38132	IN	denotes	in
T12394	38133-38138	NN	denotes	frame
T12395	38139-38143	IN	denotes	with
T12396	38144-38147	DT	denotes	the
T12398	38148-38158	JJ	denotes	endogenous
T12399	38159-38164	NN	denotes	start
T12397	38165-38168	NN	denotes	ATG
T12400	38169-38173	IN	denotes	into
T12401	38174-38178	NN	denotes	exon
T12402	38179-38180	CD	denotes	2
T12403	38181-38183	IN	denotes	of
T12404	38184-38187	DT	denotes	the
T12406	38188-38192	NN	denotes	Er81
T12407	38193-38200	JJ	denotes	genomic
T12405	38201-38206	NN	denotes	locus
T12408	38207-38210	CC	denotes	and
T12409	38211-38215	VBN	denotes	used
T12410	38216-38219	IN	denotes	for
T12411	38220-38230	JJ	denotes	homologous
T12412	38231-38244	NN	denotes	recombination
T12413	38245-38247	IN	denotes	in
T12414	38248-38251	NN	denotes	W95
T12416	38252-38254	NN	denotes	ES
T12415	38255-38260	NNS	denotes	cells
T12417	38260-38261	.	denotes	.
T12418	38261-38362	sentence	denotes	EWS-Pea3 represents a fusion gene between the amino terminal of EWS and the ETS domain of Pea3 [20].
T12419	38262-38265	NN	denotes	EWS
T12421	38265-38266	HYPH	denotes	-
T12420	38266-38270	NN	denotes	Pea3
T12422	38271-38281	VBZ	denotes	represents
T12423	38282-38283	DT	denotes	a
T12425	38284-38290	NN	denotes	fusion
T12424	38291-38295	NN	denotes	gene
T12426	38296-38303	IN	denotes	between
T12427	38304-38307	DT	denotes	the
T12429	38308-38313	NN	denotes	amino
T12428	38314-38322	NN	denotes	terminal
T12430	38323-38325	IN	denotes	of
T12431	38326-38329	NN	denotes	EWS
T12432	38330-38333	CC	denotes	and
T12433	38334-38337	DT	denotes	the
T12435	38338-38341	NN	denotes	ETS
T12434	38342-38348	NN	denotes	domain
T12436	38349-38351	IN	denotes	of
T12437	38352-38356	NN	denotes	Pea3
T12438	38357-38358	-LRB-	denotes	[
T12439	38358-38360	CD	denotes	20
T12440	38360-38361	-RRB-	denotes	]
T12441	38361-38362	.	denotes	.
T12442	38362-38498	sentence	denotes	The primer pair used to specifically detect the Er81EWS-Pea3 allele was 5′- CAGCCACTGCACCTACAAGAC-3′ and 5′- CTTCCTGCTTGATGTCTCCTTC-3′.
T12443	38363-38366	DT	denotes	The
T12445	38367-38373	NN	denotes	primer
T12444	38374-38378	NN	denotes	pair
T12447	38379-38383	VBN	denotes	used
T12448	38384-38386	TO	denotes	to
T12450	38387-38399	RB	denotes	specifically
T12449	38400-38406	VB	denotes	detect
T12451	38407-38410	DT	denotes	the
T12453	38411-38418	NN	denotes	Er81EWS
T12455	38418-38419	HYPH	denotes	-
T12454	38419-38423	NN	denotes	Pea3
T12452	38424-38430	NN	denotes	allele
T12446	38431-38434	VBD	denotes	was
T12456	38435-38436	CD	denotes	5
T12458	38436-38437	SYM	denotes	′
T12459	38437-38438	HYPH	denotes	-
T12457	38439-38460	NN	denotes	CAGCCACTGCACCTACAAGAC
T12460	38460-38461	HYPH	denotes	-
T12461	38461-38462	CD	denotes	3
T12462	38462-38463	SYM	denotes	′
T12463	38464-38467	CC	denotes	and
T12464	38468-38469	CD	denotes	5
T12466	38469-38470	SYM	denotes	′
T12467	38470-38471	HYPH	denotes	-
T12465	38472-38494	NN	denotes	CTTCCTGCTTGATGTCTCCTTC
T12468	38494-38495	HYPH	denotes	-
T12469	38495-38496	CD	denotes	3
T12470	38496-38497	SYM	denotes	′
T12471	38497-38498	.	denotes	.
T12472	38498-38798	sentence	denotes	For the generation of TaumGFP and TauEWS-Pea3 mice, lox-STOP-lox-mGFP-IRES-NLS-LacZ-pA and lox-STOP-lox-EWS-Pea3-IRES-NLS-LacZ-pA targeting cassettes were integrated into exon 2 of the Tau genomic locus (the endogenous start ATG was removed in the targeting vectors; details available upon request).
T12473	38499-38502	IN	denotes	For
T12475	38503-38506	DT	denotes	the
T12476	38507-38517	NN	denotes	generation
T12477	38518-38520	IN	denotes	of
T12478	38521-38528	NN	denotes	TaumGFP
T12480	38529-38532	CC	denotes	and
T12481	38533-38539	NN	denotes	TauEWS
T12483	38539-38540	HYPH	denotes	-
T12482	38540-38544	NN	denotes	Pea3
T12479	38545-38549	NNS	denotes	mice
T12484	38549-38551	,	denotes	,
T12485	38551-38554	NN	denotes	lox
T12487	38554-38555	HYPH	denotes	-
T12488	38555-38559	NN	denotes	STOP
T12489	38559-38560	HYPH	denotes	-
T12490	38560-38563	NN	denotes	lox
T12491	38563-38564	HYPH	denotes	-
T12492	38564-38568	NN	denotes	mGFP
T12493	38568-38569	HYPH	denotes	-
T12494	38569-38573	NN	denotes	IRES
T12495	38573-38574	HYPH	denotes	-
T12496	38574-38577	NN	denotes	NLS
T12497	38577-38578	HYPH	denotes	-
T12498	38578-38582	NN	denotes	LacZ
T12499	38582-38583	HYPH	denotes	-
T12486	38583-38585	NN	denotes	pA
T12501	38586-38589	CC	denotes	and
T12502	38590-38593	NN	denotes	lox
T12504	38593-38594	HYPH	denotes	-
T12505	38594-38598	NN	denotes	STOP
T12506	38598-38599	HYPH	denotes	-
T12507	38599-38602	NN	denotes	lox
T12508	38602-38603	HYPH	denotes	-
T12509	38603-38606	NN	denotes	EWS
T12510	38606-38607	HYPH	denotes	-
T12511	38607-38611	NN	denotes	Pea3
T12512	38611-38612	HYPH	denotes	-
T12513	38612-38616	NN	denotes	IRES
T12514	38616-38617	HYPH	denotes	-
T12515	38617-38620	NN	denotes	NLS
T12516	38620-38621	HYPH	denotes	-
T12517	38621-38625	NN	denotes	LacZ
T12518	38625-38626	HYPH	denotes	-
T12503	38626-38628	NN	denotes	pA
T12519	38629-38638	NN	denotes	targeting
T12500	38639-38648	NNS	denotes	cassettes
T12520	38649-38653	VBD	denotes	were
T12474	38654-38664	VBN	denotes	integrated
T12521	38665-38669	IN	denotes	into
T12522	38670-38674	NN	denotes	exon
T12523	38675-38676	CD	denotes	2
T12524	38677-38679	IN	denotes	of
T12525	38680-38683	DT	denotes	the
T12527	38684-38687	NN	denotes	Tau
T12528	38688-38695	JJ	denotes	genomic
T12526	38696-38701	NN	denotes	locus
T12529	38702-38703	-LRB-	denotes	(
T12531	38703-38706	DT	denotes	the
T12533	38707-38717	JJ	denotes	endogenous
T12534	38718-38723	NN	denotes	start
T12532	38724-38727	NN	denotes	ATG
T12535	38728-38731	VBD	denotes	was
T12530	38732-38739	VBN	denotes	removed
T12536	38740-38742	IN	denotes	in
T12537	38743-38746	DT	denotes	the
T12539	38747-38756	NN	denotes	targeting
T12538	38757-38764	NNS	denotes	vectors
T12540	38764-38765	:	denotes	;
T12541	38766-38773	NNS	denotes	details
T12542	38774-38783	JJ	denotes	available
T12543	38784-38788	IN	denotes	upon
T12544	38789-38796	NN	denotes	request
T12545	38796-38797	-RRB-	denotes	)
T12546	38797-38798	.	denotes	.
T12547	38798-38835	sentence	denotes	mGFP was provided by P. Caroni [25].
T12548	38799-38803	NN	denotes	mGFP
T12550	38804-38807	VBD	denotes	was
T12549	38808-38816	VBN	denotes	provided
T12551	38817-38819	IN	denotes	by
T12552	38820-38822	NNP	denotes	P.
T12553	38823-38829	NNP	denotes	Caroni
T12554	38830-38831	-LRB-	denotes	[
T12555	38831-38833	CD	denotes	25
T12556	38833-38834	-RRB-	denotes	]
T12557	38834-38835	.	denotes	.
T12558	38835-38959	sentence	denotes	ES cell recombinants were screened by Southern blot analysis using the probe in the 5′ region as described previously [41].
T12559	38836-38838	NN	denotes	ES
T12561	38839-38843	NN	denotes	cell
T12560	38844-38856	NNS	denotes	recombinants
T12563	38857-38861	VBD	denotes	were
T12562	38862-38870	VBN	denotes	screened
T12564	38871-38873	IN	denotes	by
T12565	38874-38882	NNP	denotes	Southern
T12566	38883-38887	NN	denotes	blot
T12567	38888-38896	NN	denotes	analysis
T12568	38897-38902	VBG	denotes	using
T12569	38903-38906	DT	denotes	the
T12570	38907-38912	NN	denotes	probe
T12571	38913-38915	IN	denotes	in
T12572	38916-38919	DT	denotes	the
T12574	38920-38921	CD	denotes	5
T12575	38921-38922	SYM	denotes	′
T12573	38923-38929	NN	denotes	region
T12576	38930-38932	IN	denotes	as
T12577	38933-38942	VBN	denotes	described
T12578	38943-38953	RB	denotes	previously
T12579	38954-38955	-LRB-	denotes	[
T12580	38955-38957	CD	denotes	41
T12581	38957-38958	-RRB-	denotes	]
T12582	38958-38959	.	denotes	.
T12583	38959-39053	sentence	denotes	Frequency of recombination in 129/Ola ES cells was approximately 1/3 for both Tau constructs.
T12584	38960-38969	NN	denotes	Frequency
T12586	38970-38972	IN	denotes	of
T12587	38973-38986	NN	denotes	recombination
T12588	38987-38989	IN	denotes	in
T12589	38990-38993	CD	denotes	129
T12591	38993-38994	HYPH	denotes	/
T12590	38994-38997	NN	denotes	Ola
T12593	38998-39000	NN	denotes	ES
T12592	39001-39006	NNS	denotes	cells
T12585	39007-39010	VBD	denotes	was
T12594	39011-39024	RB	denotes	approximately
T12596	39025-39026	CD	denotes	1
T12597	39026-39027	SYM	denotes	/
T12595	39027-39028	CD	denotes	3
T12598	39029-39032	IN	denotes	for
T12599	39033-39037	DT	denotes	both
T12601	39038-39041	NN	denotes	Tau
T12600	39042-39052	NNS	denotes	constructs
T12602	39052-39053	.	denotes	.
T12603	39053-39208	sentence	denotes	For the generation of PVCre mice, mouse genomic clones were obtained by screening a 129SV/J genomic library (Incyte, Wilmington, Delaware, United States).
T12604	39054-39057	IN	denotes	For
T12606	39058-39061	DT	denotes	the
T12607	39062-39072	NN	denotes	generation
T12608	39073-39075	IN	denotes	of
T12609	39076-39081	NN	denotes	PVCre
T12610	39082-39086	NNS	denotes	mice
T12611	39086-39088	,	denotes	,
T12612	39088-39093	NN	denotes	mouse
T12614	39094-39101	JJ	denotes	genomic
T12613	39102-39108	NNS	denotes	clones
T12615	39109-39113	VBD	denotes	were
T12605	39114-39122	VBN	denotes	obtained
T12616	39123-39125	IN	denotes	by
T12617	39126-39135	VBG	denotes	screening
T12618	39136-39137	DT	denotes	a
T12620	39138-39143	NN	denotes	129SV
T12622	39143-39144	HYPH	denotes	/
T12621	39144-39145	NN	denotes	J
T12623	39146-39153	JJ	denotes	genomic
T12619	39154-39161	NN	denotes	library
T12624	39162-39163	-LRB-	denotes	(
T12625	39163-39169	NNP	denotes	Incyte
T12626	39169-39171	,	denotes	,
T12627	39171-39181	NNP	denotes	Wilmington
T12628	39181-39183	,	denotes	,
T12629	39183-39191	NNP	denotes	Delaware
T12630	39191-39193	,	denotes	,
T12631	39193-39199	NNP	denotes	United
T12632	39200-39206	NNP	denotes	States
T12633	39206-39207	-RRB-	denotes	)
T12634	39207-39208	.	denotes	.
T12635	39208-39277	sentence	denotes	For details on the genomic structure of the mouse PV locus see [42].
T12636	39209-39212	IN	denotes	For
T12638	39213-39220	NNS	denotes	details
T12639	39221-39223	IN	denotes	on
T12640	39224-39227	DT	denotes	the
T12642	39228-39235	JJ	denotes	genomic
T12641	39236-39245	NN	denotes	structure
T12643	39246-39248	IN	denotes	of
T12644	39249-39252	DT	denotes	the
T12646	39253-39258	NN	denotes	mouse
T12647	39259-39261	NN	denotes	PV
T12645	39262-39267	NN	denotes	locus
T12637	39268-39271	VBP	denotes	see
T12648	39272-39273	-LRB-	denotes	[
T12649	39273-39275	CD	denotes	42
T12650	39275-39276	-RRB-	denotes	]
T12651	39276-39277	.	denotes	.
T12652	39277-39521	sentence	denotes	An IRES-Cre-pA targeting cassette [33] was integrated into the 3′ UTR of exon 5, and ES cell recombinants were screened with a 5′ probe (oligos, 5′- GAGATGACCCAGCCAGGATGCCTC-3′ and 5′- CTGACCACTCTCGCTCCGGTGTCC-3′; genomic DNA, HindIII digest).
T12653	39278-39280	DT	denotes	An
T12655	39281-39285	NN	denotes	IRES
T12657	39285-39286	HYPH	denotes	-
T12658	39286-39289	NN	denotes	Cre
T12659	39289-39290	HYPH	denotes	-
T12656	39290-39292	NN	denotes	pA
T12660	39293-39302	NN	denotes	targeting
T12654	39303-39311	NN	denotes	cassette
T12662	39312-39313	-LRB-	denotes	[
T12663	39313-39315	CD	denotes	33
T12664	39315-39316	-RRB-	denotes	]
T12665	39317-39320	VBD	denotes	was
T12661	39321-39331	VBN	denotes	integrated
T12666	39332-39336	IN	denotes	into
T12667	39337-39340	DT	denotes	the
T12669	39341-39342	CD	denotes	3
T12670	39342-39343	SYM	denotes	′
T12668	39344-39347	NN	denotes	UTR
T12671	39348-39350	IN	denotes	of
T12672	39351-39355	NN	denotes	exon
T12673	39356-39357	CD	denotes	5
T12674	39357-39359	,	denotes	,
T12675	39359-39362	CC	denotes	and
T12676	39363-39365	NN	denotes	ES
T12678	39366-39370	NN	denotes	cell
T12677	39371-39383	NNS	denotes	recombinants
T12680	39384-39388	VBD	denotes	were
T12679	39389-39397	VBN	denotes	screened
T12681	39398-39402	IN	denotes	with
T12682	39403-39404	DT	denotes	a
T12684	39405-39406	CD	denotes	5
T12685	39406-39407	SYM	denotes	′
T12683	39408-39413	NN	denotes	probe
T12686	39414-39415	-LRB-	denotes	(
T12688	39415-39421	NNS	denotes	oligos
T12689	39421-39423	,	denotes	,
T12690	39423-39424	CD	denotes	5
T12692	39424-39425	SYM	denotes	′
T12693	39425-39426	HYPH	denotes	-
T12691	39427-39451	NN	denotes	GAGATGACCCAGCCAGGATGCCTC
T12694	39451-39452	HYPH	denotes	-
T12695	39452-39453	CD	denotes	3
T12696	39453-39454	SYM	denotes	′
T12697	39455-39458	CC	denotes	and
T12698	39459-39460	CD	denotes	5
T12700	39460-39461	SYM	denotes	′
T12701	39461-39462	HYPH	denotes	-
T12699	39463-39487	NN	denotes	CTGACCACTCTCGCTCCGGTGTCC
T12702	39487-39488	HYPH	denotes	-
T12703	39488-39489	CD	denotes	3
T12704	39489-39490	SYM	denotes	′
T12705	39490-39491	:	denotes	;
T12706	39492-39499	JJ	denotes	genomic
T12707	39500-39503	NN	denotes	DNA
T12708	39503-39505	,	denotes	,
T12709	39505-39512	NN	denotes	HindIII
T12687	39513-39519	NN	denotes	digest
T12710	39519-39520	-RRB-	denotes	)
T12711	39520-39521	.	denotes	.
T12712	39521-39596	sentence	denotes	The frequency of recombination in 129/Ola ES cells was approximately 1/20.
T12713	39522-39525	DT	denotes	The
T12714	39526-39535	NN	denotes	frequency
T12716	39536-39538	IN	denotes	of
T12717	39539-39552	NN	denotes	recombination
T12718	39553-39555	IN	denotes	in
T12719	39556-39559	CD	denotes	129
T12721	39559-39560	HYPH	denotes	/
T12720	39560-39563	NN	denotes	Ola
T12723	39564-39566	NN	denotes	ES
T12722	39567-39572	NNS	denotes	cells
T12715	39573-39576	VBD	denotes	was
T12724	39577-39590	RB	denotes	approximately
T12726	39591-39592	CD	denotes	1
T12727	39592-39593	SYM	denotes	/
T12725	39593-39595	CD	denotes	20
T12728	39595-39596	.	denotes	.
T12729	39596-39728	sentence	denotes	Recombinant clones were aggregated with morula stage embryos to generate chimeric founder mice that transmitted the mutant alleles.
T12730	39597-39608	JJ	denotes	Recombinant
T12731	39609-39615	NNS	denotes	clones
T12733	39616-39620	VBD	denotes	were
T12732	39621-39631	VBN	denotes	aggregated
T12734	39632-39636	IN	denotes	with
T12735	39637-39643	NN	denotes	morula
T12736	39644-39649	NN	denotes	stage
T12737	39650-39657	NNS	denotes	embryos
T12738	39658-39660	TO	denotes	to
T12739	39661-39669	VB	denotes	generate
T12740	39670-39678	JJ	denotes	chimeric
T12742	39679-39686	NN	denotes	founder
T12741	39687-39691	NNS	denotes	mice
T12743	39692-39696	WDT	denotes	that
T12744	39697-39708	VBD	denotes	transmitted
T12745	39709-39712	DT	denotes	the
T12747	39713-39719	NN	denotes	mutant
T12746	39720-39727	NNS	denotes	alleles
T12748	39727-39728	.	denotes	.
T12749	39728-39835	sentence	denotes	In all experiments performed in this study, animals were of mixed genetic background (129/Ola and C57Bl6).
T12750	39729-39731	IN	denotes	In
T12752	39732-39735	DT	denotes	all
T12753	39736-39747	NNS	denotes	experiments
T12754	39748-39757	VBN	denotes	performed
T12755	39758-39760	IN	denotes	in
T12756	39761-39765	DT	denotes	this
T12757	39766-39771	NN	denotes	study
T12758	39771-39773	,	denotes	,
T12759	39773-39780	NNS	denotes	animals
T12751	39781-39785	VBD	denotes	were
T12760	39786-39788	IN	denotes	of
T12761	39789-39794	JJ	denotes	mixed
T12763	39795-39802	JJ	denotes	genetic
T12762	39803-39813	NN	denotes	background
T12764	39814-39815	-LRB-	denotes	(
T12766	39815-39818	CD	denotes	129
T12767	39818-39819	HYPH	denotes	/
T12765	39819-39822	NN	denotes	Ola
T12768	39823-39826	CC	denotes	and
T12769	39827-39833	NN	denotes	C57Bl6
T12770	39833-39834	-RRB-	denotes	)
T12771	39834-39835	.	denotes	.
T12772	39835-40001	sentence	denotes	Thy1spGFP transgenic mice were generated in analogy to De Paola et al. [25], and for these experiments a strain of mice with early embryonic expression was selected.
T12773	39836-39845	NN	denotes	Thy1spGFP
T12775	39846-39856	JJ	denotes	transgenic
T12774	39857-39861	NNS	denotes	mice
T12777	39862-39866	VBD	denotes	were
T12776	39867-39876	VBN	denotes	generated
T12778	39877-39879	IN	denotes	in
T12779	39880-39887	NN	denotes	analogy
T12780	39888-39890	IN	denotes	to
T12781	39891-39893	NNP	denotes	De
T12782	39894-39899	NNP	denotes	Paola
T12783	39900-39902	FW	denotes	et
T12784	39903-39906	FW	denotes	al.
T12785	39907-39908	-LRB-	denotes	[
T12786	39908-39910	CD	denotes	25
T12787	39910-39911	-RRB-	denotes	]
T12788	39911-39913	,	denotes	,
T12789	39913-39916	CC	denotes	and
T12790	39917-39920	IN	denotes	for
T12792	39921-39926	DT	denotes	these
T12793	39927-39938	NNS	denotes	experiments
T12794	39939-39940	DT	denotes	a
T12795	39941-39947	NN	denotes	strain
T12796	39948-39950	IN	denotes	of
T12797	39951-39955	NNS	denotes	mice
T12798	39956-39960	IN	denotes	with
T12799	39961-39966	JJ	denotes	early
T12801	39967-39976	JJ	denotes	embryonic
T12800	39977-39987	NN	denotes	expression
T12802	39988-39991	VBD	denotes	was
T12791	39992-40000	VBN	denotes	selected
T12803	40000-40001	.	denotes	.
T12804	40001-40151	sentence	denotes	Isl1Cre and Hb9Cre mouse strains have been described [33,43] and Bax+/− animals were from Jackson Laboratory (Bar Harbor, Maine, United States) [27].
T12805	40002-40009	NN	denotes	Isl1Cre
T12807	40010-40013	CC	denotes	and
T12808	40014-40020	NN	denotes	Hb9Cre
T12809	40021-40026	NN	denotes	mouse
T12806	40027-40034	NNS	denotes	strains
T12811	40035-40039	VBP	denotes	have
T12812	40040-40044	VBN	denotes	been
T12810	40045-40054	VBN	denotes	described
T12813	40055-40056	-LRB-	denotes	[
T12815	40056-40058	CD	denotes	33
T12816	40058-40059	,	denotes	,
T12814	40059-40061	CD	denotes	43
T12817	40061-40062	-RRB-	denotes	]
T12818	40063-40066	CC	denotes	and
T12819	40067-40070	NN	denotes	Bax
T12821	40070-40071	SYM	denotes	+
T12822	40071-40072	HYPH	denotes	/
T12823	40072-40073	SYM	denotes	−
T12820	40074-40081	NNS	denotes	animals
T12824	40082-40086	VBD	denotes	were
T12825	40087-40091	IN	denotes	from
T12826	40092-40099	NNP	denotes	Jackson
T12827	40100-40110	NNP	denotes	Laboratory
T12828	40111-40112	-LRB-	denotes	(
T12830	40112-40115	NNP	denotes	Bar
T12829	40116-40122	NNP	denotes	Harbor
T12831	40122-40124	,	denotes	,
T12832	40124-40129	NNP	denotes	Maine
T12833	40129-40131	,	denotes	,
T12834	40131-40137	NNP	denotes	United
T12835	40138-40144	NNP	denotes	States
T12836	40144-40145	-RRB-	denotes	)
T12837	40146-40147	-LRB-	denotes	[
T12838	40147-40149	CD	denotes	27
T12839	40149-40150	-RRB-	denotes	]
T12840	40150-40151	.	denotes	.
T12841	40151-40286	sentence	denotes	Timed pregnancies were set up to generate embryos of different developmental stages with all genotypes described throughout the study.
T12842	40152-40157	JJ	denotes	Timed
T12843	40158-40169	NNS	denotes	pregnancies
T12845	40170-40174	VBD	denotes	were
T12844	40175-40178	VBN	denotes	set
T12846	40179-40181	RP	denotes	up
T12847	40182-40184	TO	denotes	to
T12848	40185-40193	VB	denotes	generate
T12849	40194-40201	NNS	denotes	embryos
T12850	40202-40204	IN	denotes	of
T12851	40205-40214	JJ	denotes	different
T12853	40215-40228	JJ	denotes	developmental
T12852	40229-40235	NNS	denotes	stages
T12854	40236-40240	IN	denotes	with
T12856	40241-40244	DT	denotes	all
T12857	40245-40254	NNS	denotes	genotypes
T12855	40255-40264	VBN	denotes	described
T12858	40265-40275	IN	denotes	throughout
T12859	40276-40279	DT	denotes	the
T12860	40280-40285	NN	denotes	study
T12861	40285-40286	.	denotes	.
T13099	40288-40303	JJ	denotes	Transcriptional
T13100	40304-40319	NN	denotes	transactivation
T13101	40320-40326	NNS	denotes	assays
T13102	40326-40523	sentence	denotes	The following plasmids were used for transcriptional transactivation assays: pRc/RSV (Invitrogen, Carlsbad, California, United States), pRc/RSV-Er81, pRc/RSV-EWS-Pea3, pTP-5xETS, and pTP-5xETSmut.
T13103	40327-40330	DT	denotes	The
T13105	40331-40340	VBG	denotes	following
T13104	40341-40349	NNS	denotes	plasmids
T13107	40350-40354	VBD	denotes	were
T13106	40355-40359	VBN	denotes	used
T13108	40360-40363	IN	denotes	for
T13109	40364-40379	JJ	denotes	transcriptional
T13111	40380-40395	NN	denotes	transactivation
T13110	40396-40402	NNS	denotes	assays
T13112	40402-40404	:	denotes	:
T13113	40404-40407	NN	denotes	pRc
T13115	40407-40408	HYPH	denotes	/
T13114	40408-40411	NN	denotes	RSV
T13116	40412-40413	-LRB-	denotes	(
T13117	40413-40423	NNP	denotes	Invitrogen
T13118	40423-40425	,	denotes	,
T13119	40425-40433	NNP	denotes	Carlsbad
T13120	40433-40435	,	denotes	,
T13121	40435-40445	NNP	denotes	California
T13122	40445-40447	,	denotes	,
T13123	40447-40453	NNP	denotes	United
T13124	40454-40460	NNP	denotes	States
T13125	40460-40461	-RRB-	denotes	)
T13126	40461-40463	,	denotes	,
T13127	40463-40466	NN	denotes	pRc
T13129	40466-40467	HYPH	denotes	/
T13128	40467-40470	NN	denotes	RSV
T13131	40470-40471	HYPH	denotes	-
T13130	40471-40475	NN	denotes	Er81
T13132	40475-40477	,	denotes	,
T13133	40477-40480	NN	denotes	pRc
T13135	40480-40481	HYPH	denotes	/
T13134	40481-40484	NN	denotes	RSV
T13137	40484-40485	HYPH	denotes	-
T13138	40485-40488	NN	denotes	EWS
T13139	40488-40489	HYPH	denotes	-
T13136	40489-40493	NN	denotes	Pea3
T13140	40493-40495	,	denotes	,
T13141	40495-40498	NN	denotes	pTP
T13143	40498-40499	HYPH	denotes	-
T13142	40499-40504	NN	denotes	5xETS
T13144	40504-40506	,	denotes	,
T13145	40506-40509	CC	denotes	and
T13146	40510-40513	NN	denotes	pTP
T13148	40513-40514	HYPH	denotes	-
T13147	40514-40522	NN	denotes	5xETSmut
T13149	40522-40523	.	denotes	.
T13150	40523-40658	sentence	denotes	pRc/RSV-Er81 and pRc/RSV-EWS-Pea3 were obtained by insertion of the cDNAs for Er81 or EWS-Pea3 (gift from J. A. Hassell) into pRc/RSV.
T13151	40524-40527	NN	denotes	pRc
T13153	40527-40528	HYPH	denotes	/
T13152	40528-40531	NN	denotes	RSV
T13155	40531-40532	HYPH	denotes	-
T13154	40532-40536	NN	denotes	Er81
T13157	40537-40540	CC	denotes	and
T13158	40541-40544	NN	denotes	pRc
T13160	40544-40545	HYPH	denotes	/
T13159	40545-40548	NN	denotes	RSV
T13162	40548-40549	HYPH	denotes	-
T13163	40549-40552	NN	denotes	EWS
T13164	40552-40553	HYPH	denotes	-
T13161	40553-40557	NN	denotes	Pea3
T13165	40558-40562	VBD	denotes	were
T13156	40563-40571	VBN	denotes	obtained
T13166	40572-40574	IN	denotes	by
T13167	40575-40584	NN	denotes	insertion
T13168	40585-40587	IN	denotes	of
T13169	40588-40591	DT	denotes	the
T13170	40592-40597	NNS	denotes	cDNAs
T13171	40598-40601	IN	denotes	for
T13172	40602-40606	NN	denotes	Er81
T13173	40607-40609	CC	denotes	or
T13174	40610-40613	NN	denotes	EWS
T13176	40613-40614	HYPH	denotes	-
T13175	40614-40618	NN	denotes	Pea3
T13177	40619-40620	-LRB-	denotes	(
T13178	40620-40624	NN	denotes	gift
T13179	40625-40629	IN	denotes	from
T13180	40630-40632	NNP	denotes	J.
T13182	40633-40635	NNP	denotes	A.
T13181	40636-40643	NNP	denotes	Hassell
T13183	40643-40644	-RRB-	denotes	)
T13184	40645-40649	IN	denotes	into
T13185	40650-40653	NN	denotes	pRc
T13187	40653-40654	HYPH	denotes	/
T13186	40654-40657	NN	denotes	RSV
T13188	40657-40658	.	denotes	.
T13189	40658-40833	sentence	denotes	pTP-5xETS was constructed by inserting a cassette of five repetitive copies of high-affinity Pea3 binding sites (5′- GCCGGAAGC-3′) [18,19] into a modified version of pTK-Luc.
T13190	40659-40662	NN	denotes	pTP
T13192	40662-40663	HYPH	denotes	-
T13191	40663-40668	NN	denotes	5xETS
T13194	40669-40672	VBD	denotes	was
T13193	40673-40684	VBN	denotes	constructed
T13195	40685-40687	IN	denotes	by
T13196	40688-40697	VBG	denotes	inserting
T13197	40698-40699	DT	denotes	a
T13198	40700-40708	NN	denotes	cassette
T13199	40709-40711	IN	denotes	of
T13200	40712-40716	CD	denotes	five
T13202	40717-40727	JJ	denotes	repetitive
T13201	40728-40734	NNS	denotes	copies
T13203	40735-40737	IN	denotes	of
T13204	40738-40742	JJ	denotes	high
T13206	40742-40743	HYPH	denotes	-
T13205	40743-40751	NN	denotes	affinity
T13208	40752-40756	NN	denotes	Pea3
T13209	40757-40764	NN	denotes	binding
T13207	40765-40770	NNS	denotes	sites
T13210	40771-40772	-LRB-	denotes	(
T13212	40772-40773	CD	denotes	5
T13213	40773-40774	SYM	denotes	′
T13214	40774-40775	HYPH	denotes	-
T13215	40776-40785	NN	denotes	GCCGGAAGC
T13216	40785-40786	HYPH	denotes	-
T13211	40786-40787	CD	denotes	3
T13217	40787-40788	SYM	denotes	′
T13218	40788-40789	-RRB-	denotes	)
T13219	40790-40791	-LRB-	denotes	[
T13221	40791-40793	CD	denotes	18
T13222	40793-40794	,	denotes	,
T13220	40794-40796	CD	denotes	19
T13223	40796-40797	-RRB-	denotes	]
T13224	40798-40802	IN	denotes	into
T13225	40803-40804	DT	denotes	a
T13227	40805-40813	VBN	denotes	modified
T13226	40814-40821	NN	denotes	version
T13228	40822-40824	IN	denotes	of
T13229	40825-40828	NN	denotes	pTK
T13231	40828-40829	HYPH	denotes	-
T13230	40829-40832	NN	denotes	Luc
T13232	40832-40833	.	denotes	.
T13233	40833-40950	sentence	denotes	pTP-5xETSmut was generated as pTP-5xETS but using a mutated complement of the Pea3 binding sites (5′- GCCTATGGC-3′).
T13234	40834-40837	NN	denotes	pTP
T13236	40837-40838	HYPH	denotes	-
T13235	40838-40846	NN	denotes	5xETSmut
T13238	40847-40850	VBD	denotes	was
T13237	40851-40860	VBN	denotes	generated
T13239	40861-40863	IN	denotes	as
T13240	40864-40867	NN	denotes	pTP
T13242	40867-40868	HYPH	denotes	-
T13241	40868-40873	NN	denotes	5xETS
T13243	40874-40877	CC	denotes	but
T13244	40878-40883	VBG	denotes	using
T13245	40884-40885	DT	denotes	a
T13247	40886-40893	VBN	denotes	mutated
T13246	40894-40904	NN	denotes	complement
T13248	40905-40907	IN	denotes	of
T13249	40908-40911	DT	denotes	the
T13251	40912-40916	NN	denotes	Pea3
T13252	40917-40924	NN	denotes	binding
T13250	40925-40930	NNS	denotes	sites
T13253	40931-40932	-LRB-	denotes	(
T13255	40932-40933	CD	denotes	5
T13256	40933-40934	SYM	denotes	′
T13257	40934-40935	HYPH	denotes	-
T13258	40936-40945	NN	denotes	GCCTATGGC
T13259	40945-40946	HYPH	denotes	-
T13254	40946-40947	CD	denotes	3
T13260	40947-40948	SYM	denotes	′
T13261	40948-40949	-RRB-	denotes	)
T13262	40949-40950	.	denotes	.
T13263	40950-41059	sentence	denotes	A control plasmid to normalize for transfection efficiency (placZ) and pTK-Luc were a gift from D. Kressler.
T13264	40951-40952	DT	denotes	A
T13266	40953-40960	NN	denotes	control
T13265	40961-40968	NN	denotes	plasmid
T13268	40969-40971	TO	denotes	to
T13269	40972-40981	VB	denotes	normalize
T13270	40982-40985	IN	denotes	for
T13271	40986-40998	NN	denotes	transfection
T13272	40999-41009	NN	denotes	efficiency
T13273	41010-41011	-LRB-	denotes	(
T13274	41011-41016	NN	denotes	placZ
T13275	41016-41017	-RRB-	denotes	)
T13276	41018-41021	CC	denotes	and
T13277	41022-41025	NN	denotes	pTK
T13279	41025-41026	HYPH	denotes	-
T13278	41026-41029	NN	denotes	Luc
T13267	41030-41034	VBD	denotes	were
T13280	41035-41036	DT	denotes	a
T13281	41037-41041	NN	denotes	gift
T13282	41042-41046	IN	denotes	from
T13283	41047-41049	NNP	denotes	D.
T13284	41050-41058	NNP	denotes	Kressler
T13285	41058-41059	.	denotes	.
T13286	41059-41274	sentence	denotes	COS-7 cells were co-transfected with 1–1.2 μg of total DNA including one of the effector plasmids pRc/RSV-empty, pRc/RSV-Er81, or pRc/RSV-EWS-Pea3; one of the reporter plasmids pTP-5xETS or pTP-5xETSmut; and placZ.
T13287	41060-41063	NN	denotes	COS
T13289	41063-41064	HYPH	denotes	-
T13290	41064-41065	CD	denotes	7
T13288	41066-41071	NNS	denotes	cells
T13292	41072-41076	VBD	denotes	were
T13291	41077-41091	VBN	denotes	co-transfected
T13293	41092-41096	IN	denotes	with
T13294	41097-41098	CD	denotes	1
T13296	41098-41099	SYM	denotes	–
T13295	41099-41102	CD	denotes	1.2
T13297	41103-41105	NN	denotes	μg
T13298	41106-41108	IN	denotes	of
T13299	41109-41114	JJ	denotes	total
T13300	41115-41118	NN	denotes	DNA
T13301	41119-41128	VBG	denotes	including
T13302	41129-41132	CD	denotes	one
T13303	41133-41135	IN	denotes	of
T13304	41136-41139	DT	denotes	the
T13306	41140-41148	NN	denotes	effector
T13305	41149-41157	NNS	denotes	plasmids
T13307	41158-41161	NN	denotes	pRc
T13309	41161-41162	HYPH	denotes	/
T13308	41162-41165	NN	denotes	RSV
T13310	41165-41166	HYPH	denotes	-
T13311	41166-41171	JJ	denotes	empty
T13312	41171-41173	,	denotes	,
T13313	41173-41176	NN	denotes	pRc
T13315	41176-41177	HYPH	denotes	/
T13314	41177-41180	NN	denotes	RSV
T13317	41180-41181	HYPH	denotes	-
T13316	41181-41185	NN	denotes	Er81
T13318	41185-41187	,	denotes	,
T13319	41187-41189	CC	denotes	or
T13320	41190-41193	NN	denotes	pRc
T13322	41193-41194	HYPH	denotes	/
T13321	41194-41197	NN	denotes	RSV
T13324	41197-41198	HYPH	denotes	-
T13325	41198-41201	NN	denotes	EWS
T13326	41201-41202	HYPH	denotes	-
T13323	41202-41206	NN	denotes	Pea3
T13327	41206-41207	:	denotes	;
T13328	41208-41211	CD	denotes	one
T13329	41212-41214	IN	denotes	of
T13330	41215-41218	DT	denotes	the
T13332	41219-41227	NN	denotes	reporter
T13331	41228-41236	NNS	denotes	plasmids
T13333	41237-41240	NN	denotes	pTP
T13335	41240-41241	HYPH	denotes	-
T13334	41241-41246	NN	denotes	5xETS
T13336	41247-41249	CC	denotes	or
T13337	41250-41253	NN	denotes	pTP
T13339	41253-41254	HYPH	denotes	-
T13338	41254-41262	NN	denotes	5xETSmut
T13340	41262-41263	:	denotes	;
T13341	41264-41267	CC	denotes	and
T13342	41268-41273	NN	denotes	placZ
T13343	41273-41274	.	denotes	.
T13344	41274-41403	sentence	denotes	Cells were harvested after 25 h and processed for assays to determine luciferase and LacZ activity as described previously [44].
T13345	41275-41280	NNS	denotes	Cells
T13347	41281-41285	VBD	denotes	were
T13346	41286-41295	VBN	denotes	harvested
T13348	41296-41301	IN	denotes	after
T13349	41302-41304	CD	denotes	25
T13350	41305-41306	NN	denotes	h
T13351	41307-41310	CC	denotes	and
T13352	41311-41320	VBN	denotes	processed
T13353	41321-41324	IN	denotes	for
T13354	41325-41331	NNS	denotes	assays
T13355	41332-41334	TO	denotes	to
T13356	41335-41344	VB	denotes	determine
T13357	41345-41355	NN	denotes	luciferase
T13359	41356-41359	CC	denotes	and
T13360	41360-41364	NN	denotes	LacZ
T13358	41365-41373	NN	denotes	activity
T13361	41374-41376	IN	denotes	as
T13362	41377-41386	VBN	denotes	described
T13363	41387-41397	RB	denotes	previously
T13364	41398-41399	-LRB-	denotes	[
T13365	41399-41401	CD	denotes	44
T13366	41401-41402	-RRB-	denotes	]
T13367	41402-41403	.	denotes	.
T13368	41403-41486	sentence	denotes	Luciferase values normalized to LacZ activity are referred to as luciferase units.
T13369	41404-41414	NN	denotes	Luciferase
T13370	41415-41421	NNS	denotes	values
T13372	41422-41432	VBN	denotes	normalized
T13373	41433-41435	IN	denotes	to
T13374	41436-41440	NN	denotes	LacZ
T13375	41441-41449	NN	denotes	activity
T13376	41450-41453	VBP	denotes	are
T13371	41454-41462	VBN	denotes	referred
T13377	41463-41465	IN	denotes	to
T13378	41466-41468	IN	denotes	as
T13379	41469-41479	NN	denotes	luciferase
T13380	41480-41485	NNS	denotes	units
T13381	41485-41486	.	denotes	.
T13917	41488-41490	FW	denotes	In
T13918	41491-41495	FW	denotes	situ
T13919	41496-41509	NN	denotes	hybridization
T13920	41510-41513	CC	denotes	and
T13921	41514-41534	NN	denotes	immunohistochemistry
T13922	41534-41717	sentence	denotes	For in situ hybridization analysis, cryostat sections were hybridized using digoxigenin-labeled probes [45] directed against mouse TrkA or TrkB, or rat TrkC (gift from L. F. Parada).
T13923	41535-41538	IN	denotes	For
T13925	41539-41541	FW	denotes	in
T13926	41542-41546	FW	denotes	situ
T13928	41547-41560	NN	denotes	hybridization
T13927	41561-41569	NN	denotes	analysis
T13929	41569-41571	,	denotes	,
T13930	41571-41579	NN	denotes	cryostat
T13931	41580-41588	NNS	denotes	sections
T13932	41589-41593	VBD	denotes	were
T13924	41594-41604	VBN	denotes	hybridized
T13933	41605-41610	VBG	denotes	using
T13934	41611-41622	NN	denotes	digoxigenin
T13936	41622-41623	HYPH	denotes	-
T13935	41623-41630	VBN	denotes	labeled
T13937	41631-41637	NNS	denotes	probes
T13938	41638-41639	-LRB-	denotes	[
T13939	41639-41641	CD	denotes	45
T13940	41641-41642	-RRB-	denotes	]
T13941	41643-41651	VBN	denotes	directed
T13942	41652-41659	IN	denotes	against
T13943	41660-41665	NN	denotes	mouse
T13944	41666-41670	NN	denotes	TrkA
T13945	41671-41673	CC	denotes	or
T13946	41674-41678	NN	denotes	TrkB
T13947	41678-41680	,	denotes	,
T13948	41680-41682	CC	denotes	or
T13949	41683-41686	NN	denotes	rat
T13950	41687-41691	NN	denotes	TrkC
T13951	41692-41693	-LRB-	denotes	(
T13952	41693-41697	NN	denotes	gift
T13953	41698-41702	IN	denotes	from
T13954	41703-41705	NNP	denotes	L.
T13956	41706-41708	NNP	denotes	F.
T13955	41709-41715	NNP	denotes	Parada
T13957	41715-41716	-RRB-	denotes	)
T13958	41716-41717	.	denotes	.
T13959	41717-42547	sentence	denotes	Antibodies used in this study were as follows: rabbit anti-Er81 [14], rabbit anti-Pea3 [14], rabbit anti-PV [14], rabbit anti-eGFP (Molecular Probes, Eugene, Oregon, United States), rabbit anti-Calbindin, rabbit anti-Calretinin (Swant, Bellinzona, Switzerland), rabbit anti-CGRP (Chemicon, Temecula, California, United States), rabbit anti-vGlut1 (Synaptic Systems, Goettingen, Germany), rabbit anti-Brn3a (gift from E. Turner), rabbit anti-TrkA and -p75 (gift from L. F. Reichardt), rabbit anti-Runx3 (Kramer and Arber, unpublished reagent), rabbit anti-Rhodamine (Molecular Probes), mouse anti-neurofilament (American Type Culture Collection, Manassas, Virginia, United States), sheep anti-eGFP (Biogenesis, Poole, United Kingdom), goat anti-LacZ [14], goat anti-TrkC (gift from L. F. Reichardt), and guinea pig anti-Isl1 [14].
T13960	41718-41728	NNS	denotes	Antibodies
T13962	41729-41733	VBN	denotes	used
T13963	41734-41736	IN	denotes	in
T13964	41737-41741	DT	denotes	this
T13965	41742-41747	NN	denotes	study
T13961	41748-41752	VBD	denotes	were
T13966	41753-41755	IN	denotes	as
T13967	41756-41763	VBZ	denotes	follows
T13968	41763-41765	:	denotes	:
T13969	41765-41771	NN	denotes	rabbit
T13970	41772-41781	NN	denotes	anti-Er81
T13971	41782-41783	-LRB-	denotes	[
T13972	41783-41785	CD	denotes	14
T13973	41785-41786	-RRB-	denotes	]
T13974	41786-41788	,	denotes	,
T13975	41788-41794	NN	denotes	rabbit
T13976	41795-41804	JJ	denotes	anti-Pea3
T13977	41805-41806	-LRB-	denotes	[
T13978	41806-41808	CD	denotes	14
T13979	41808-41809	-RRB-	denotes	]
T13980	41809-41811	,	denotes	,
T13981	41811-41817	NN	denotes	rabbit
T13982	41818-41825	JJ	denotes	anti-PV
T13983	41826-41827	-LRB-	denotes	[
T13984	41827-41829	CD	denotes	14
T13985	41829-41830	-RRB-	denotes	]
T13986	41830-41832	,	denotes	,
T13987	41832-41838	NN	denotes	rabbit
T13988	41839-41848	JJ	denotes	anti-eGFP
T13989	41849-41850	-LRB-	denotes	(
T13991	41850-41859	NNP	denotes	Molecular
T13990	41860-41866	NNP	denotes	Probes
T13992	41866-41868	,	denotes	,
T13993	41868-41874	NNP	denotes	Eugene
T13994	41874-41876	,	denotes	,
T13995	41876-41882	NNP	denotes	Oregon
T13996	41882-41884	,	denotes	,
T13997	41884-41890	NNP	denotes	United
T13998	41891-41897	NNP	denotes	States
T13999	41897-41898	-RRB-	denotes	)
T14000	41898-41900	,	denotes	,
T14001	41900-41906	NN	denotes	rabbit
T14002	41907-41921	JJ	denotes	anti-Calbindin
T14003	41921-41923	,	denotes	,
T14004	41923-41929	NN	denotes	rabbit
T14005	41930-41945	JJ	denotes	anti-Calretinin
T14006	41946-41947	-LRB-	denotes	(
T14007	41947-41952	NNP	denotes	Swant
T14008	41952-41954	,	denotes	,
T14009	41954-41964	NNP	denotes	Bellinzona
T14010	41964-41966	,	denotes	,
T14011	41966-41977	NNP	denotes	Switzerland
T14012	41977-41978	-RRB-	denotes	)
T14013	41978-41980	,	denotes	,
T14014	41980-41986	NN	denotes	rabbit
T14015	41987-41996	JJ	denotes	anti-CGRP
T14016	41997-41998	-LRB-	denotes	(
T14017	41998-42006	NNP	denotes	Chemicon
T14018	42006-42008	,	denotes	,
T14019	42008-42016	NNP	denotes	Temecula
T14020	42016-42018	,	denotes	,
T14021	42018-42028	NNP	denotes	California
T14022	42028-42030	,	denotes	,
T14023	42030-42036	NNP	denotes	United
T14024	42037-42043	NNP	denotes	States
T14025	42043-42044	-RRB-	denotes	)
T14026	42044-42046	,	denotes	,
T14027	42046-42052	NN	denotes	rabbit
T14028	42053-42064	JJ	denotes	anti-vGlut1
T14029	42065-42066	-LRB-	denotes	(
T14031	42066-42074	NN	denotes	Synaptic
T14030	42075-42082	NNP	denotes	Systems
T14032	42082-42084	,	denotes	,
T14033	42084-42094	NNP	denotes	Goettingen
T14034	42094-42096	,	denotes	,
T14035	42096-42103	NNP	denotes	Germany
T14036	42103-42104	-RRB-	denotes	)
T14037	42104-42106	,	denotes	,
T14038	42106-42112	NN	denotes	rabbit
T14039	42113-42123	JJ	denotes	anti-Brn3a
T14040	42124-42125	-LRB-	denotes	(
T14041	42125-42129	NN	denotes	gift
T14042	42130-42134	IN	denotes	from
T14043	42135-42137	NNP	denotes	E.
T14044	42138-42144	NNP	denotes	Turner
T14045	42144-42145	-RRB-	denotes	)
T14046	42145-42147	,	denotes	,
T14047	42147-42153	NN	denotes	rabbit
T14049	42154-42163	JJ	denotes	anti-TrkA
T14050	42164-42167	CC	denotes	and
T14051	42168-42169	HYPH	denotes	-
T14048	42169-42172	NN	denotes	p75
T14052	42173-42174	-LRB-	denotes	(
T14053	42174-42178	NN	denotes	gift
T14054	42179-42183	IN	denotes	from
T14055	42184-42186	NNP	denotes	L.
T14057	42187-42189	NNP	denotes	F.
T14056	42190-42199	NNP	denotes	Reichardt
T14058	42199-42200	-RRB-	denotes	)
T14059	42200-42202	,	denotes	,
T14060	42202-42208	NN	denotes	rabbit
T14061	42209-42219	JJ	denotes	anti-Runx3
T14062	42220-42221	-LRB-	denotes	(
T14063	42221-42227	NNP	denotes	Kramer
T14064	42228-42231	CC	denotes	and
T14065	42232-42237	NNP	denotes	Arber
T14066	42237-42239	,	denotes	,
T14067	42239-42250	JJ	denotes	unpublished
T14068	42251-42258	NN	denotes	reagent
T14069	42258-42259	-RRB-	denotes	)
T14070	42259-42261	,	denotes	,
T14071	42261-42267	NN	denotes	rabbit
T14072	42268-42282	JJ	denotes	anti-Rhodamine
T14073	42283-42284	-LRB-	denotes	(
T14075	42284-42293	NNP	denotes	Molecular
T14074	42294-42300	NNP	denotes	Probes
T14076	42300-42301	-RRB-	denotes	)
T14077	42301-42303	,	denotes	,
T14078	42303-42308	NN	denotes	mouse
T14079	42309-42327	JJ	denotes	anti-neurofilament
T14080	42328-42329	-LRB-	denotes	(
T14082	42329-42337	NNP	denotes	American
T14083	42338-42342	NNP	denotes	Type
T14084	42343-42350	NNP	denotes	Culture
T14081	42351-42361	NNP	denotes	Collection
T14085	42361-42363	,	denotes	,
T14086	42363-42371	NNP	denotes	Manassas
T14087	42371-42373	,	denotes	,
T14088	42373-42381	NNP	denotes	Virginia
T14089	42381-42383	,	denotes	,
T14090	42383-42389	NNP	denotes	United
T14091	42390-42396	NNP	denotes	States
T14092	42396-42397	-RRB-	denotes	)
T14093	42397-42399	,	denotes	,
T14094	42399-42404	NN	denotes	sheep
T14095	42405-42414	JJ	denotes	anti-eGFP
T14096	42415-42416	-LRB-	denotes	(
T14097	42416-42426	NNP	denotes	Biogenesis
T14098	42426-42428	,	denotes	,
T14099	42428-42433	NNP	denotes	Poole
T14100	42433-42435	,	denotes	,
T14101	42435-42441	NNP	denotes	United
T14102	42442-42449	NNP	denotes	Kingdom
T14103	42449-42450	-RRB-	denotes	)
T14104	42450-42452	,	denotes	,
T14105	42452-42456	NN	denotes	goat
T14106	42457-42466	JJ	denotes	anti-LacZ
T14107	42467-42468	-LRB-	denotes	[
T14108	42468-42470	CD	denotes	14
T14109	42470-42471	-RRB-	denotes	]
T14110	42471-42473	,	denotes	,
T14111	42473-42477	NN	denotes	goat
T14112	42478-42487	JJ	denotes	anti-TrkC
T14113	42488-42489	-LRB-	denotes	(
T14114	42489-42493	NN	denotes	gift
T14115	42494-42498	IN	denotes	from
T14116	42499-42501	NNP	denotes	L.
T14118	42502-42504	NNP	denotes	F.
T14117	42505-42514	NNP	denotes	Reichardt
T14119	42514-42515	-RRB-	denotes	)
T14120	42515-42517	,	denotes	,
T14121	42517-42520	CC	denotes	and
T14122	42521-42527	NN	denotes	guinea
T14123	42528-42531	NN	denotes	pig
T14124	42532-42541	JJ	denotes	anti-Isl1
T14125	42542-42543	-LRB-	denotes	[
T14126	42543-42545	CD	denotes	14
T14127	42545-42546	-RRB-	denotes	]
T14128	42546-42547	.	denotes	.
T14129	42547-42773	sentence	denotes	Terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL) to detect apoptotic cells in E13.5 DRG on cryostat sections was performed as described by the manufacturer (Roche, Basel, Switzerland).
T14130	42548-42556	JJ	denotes	Terminal
T14132	42557-42573	NN	denotes	deoxynucleotidyl
T14131	42574-42585	NN	denotes	transferase
T14134	42585-42586	HYPH	denotes	-
T14133	42586-42594	VBN	denotes	mediated
T14136	42595-42607	VBN	denotes	biotinylated
T14137	42608-42611	NN	denotes	UTP
T14138	42612-42616	NN	denotes	nick
T14139	42617-42620	NN	denotes	end
T14135	42621-42629	NN	denotes	labeling
T14141	42630-42631	-LRB-	denotes	(
T14142	42631-42636	NN	denotes	TUNEL
T14143	42636-42637	-RRB-	denotes	)
T14144	42638-42640	TO	denotes	to
T14145	42641-42647	VB	denotes	detect
T14146	42648-42657	JJ	denotes	apoptotic
T14147	42658-42663	NNS	denotes	cells
T14148	42664-42666	IN	denotes	in
T14149	42667-42672	NN	denotes	E13.5
T14150	42673-42676	NN	denotes	DRG
T14151	42677-42679	IN	denotes	on
T14152	42680-42688	NN	denotes	cryostat
T14153	42689-42697	NNS	denotes	sections
T14154	42698-42701	VBD	denotes	was
T14140	42702-42711	VBN	denotes	performed
T14155	42712-42714	IN	denotes	as
T14156	42715-42724	VBN	denotes	described
T14157	42725-42727	IN	denotes	by
T14158	42728-42731	DT	denotes	the
T14159	42732-42744	NN	denotes	manufacturer
T14160	42745-42746	-LRB-	denotes	(
T14161	42746-42751	NNP	denotes	Roche
T14162	42751-42753	,	denotes	,
T14163	42753-42758	NNP	denotes	Basel
T14164	42758-42760	,	denotes	,
T14165	42760-42771	NNP	denotes	Switzerland
T14166	42771-42772	-RRB-	denotes	)
T14167	42772-42773	.	denotes	.
T14168	42773-42860	sentence	denotes	Quantitative analysis of TUNEL+ DRG cells was performed essentially as described [27].
T14169	42774-42786	JJ	denotes	Quantitative
T14170	42787-42795	NN	denotes	analysis
T14172	42796-42798	IN	denotes	of
T14173	42799-42804	NN	denotes	TUNEL
T14175	42804-42805	SYM	denotes	+
T14176	42806-42809	NN	denotes	DRG
T14174	42810-42815	NNS	denotes	cells
T14177	42816-42819	VBD	denotes	was
T14171	42820-42829	VBN	denotes	performed
T14178	42830-42841	RB	denotes	essentially
T14180	42842-42844	IN	denotes	as
T14179	42845-42854	VBN	denotes	described
T14181	42855-42856	-LRB-	denotes	[
T14182	42856-42858	CD	denotes	27
T14183	42858-42859	-RRB-	denotes	]
T14184	42859-42860	.	denotes	.
T14185	42860-42964	sentence	denotes	BrdU pulse-chase experiments and LacZ wholemount stainings were performed as previously described [46].
T14186	42861-42865	NN	denotes	BrdU
T14188	42866-42871	NN	denotes	pulse
T14190	42871-42872	HYPH	denotes	-
T14189	42872-42877	NN	denotes	chase
T14187	42878-42889	NNS	denotes	experiments
T14192	42890-42893	CC	denotes	and
T14193	42894-42898	NN	denotes	LacZ
T14195	42899-42909	NN	denotes	wholemount
T14194	42910-42919	NNS	denotes	stainings
T14196	42920-42924	VBD	denotes	were
T14191	42925-42934	VBN	denotes	performed
T14197	42935-42937	IN	denotes	as
T14199	42938-42948	RB	denotes	previously
T14198	42949-42958	VBN	denotes	described
T14200	42959-42960	-LRB-	denotes	[
T14201	42960-42962	CD	denotes	46
T14202	42962-42963	-RRB-	denotes	]
T14203	42963-42964	.	denotes	.
T14204	42964-43236	sentence	denotes	For anterograde tracing experiments to visualize projections of sensory neurons, rhodamine-conjugated dextran (Molecular Probes) was injected into single lumbar (L3) DRG at E13.5 or applied to whole lumbar dorsal roots (L3) at postnatal day (P) 5 using glass capillaries.
T14205	42965-42968	IN	denotes	For
T14207	42969-42980	JJ	denotes	anterograde
T14209	42981-42988	NN	denotes	tracing
T14208	42989-43000	NNS	denotes	experiments
T14210	43001-43003	TO	denotes	to
T14206	43004-43013	VB	denotes	visualize
T14212	43014-43025	NNS	denotes	projections
T14213	43026-43028	IN	denotes	of
T14214	43029-43036	JJ	denotes	sensory
T14215	43037-43044	NNS	denotes	neurons
T14216	43044-43046	,	denotes	,
T14217	43046-43055	NN	denotes	rhodamine
T14219	43055-43056	HYPH	denotes	-
T14218	43056-43066	VBN	denotes	conjugated
T14220	43067-43074	NN	denotes	dextran
T14221	43075-43076	-LRB-	denotes	(
T14223	43076-43085	NNP	denotes	Molecular
T14222	43086-43092	NNP	denotes	Probes
T14224	43092-43093	-RRB-	denotes	)
T14225	43094-43097	VBD	denotes	was
T14211	43098-43106	VBN	denotes	injected
T14226	43107-43111	IN	denotes	into
T14227	43112-43118	JJ	denotes	single
T14229	43119-43125	NN	denotes	lumbar
T14230	43126-43127	-LRB-	denotes	(
T14231	43127-43129	NN	denotes	L3
T14232	43129-43130	-RRB-	denotes	)
T14228	43131-43134	NN	denotes	DRG
T14233	43135-43137	IN	denotes	at
T14234	43138-43143	NN	denotes	E13.5
T14235	43144-43146	CC	denotes	or
T14236	43147-43154	VBN	denotes	applied
T14237	43155-43157	IN	denotes	to
T14238	43158-43163	JJ	denotes	whole
T14240	43164-43170	JJ	denotes	lumbar
T14241	43171-43177	JJ	denotes	dorsal
T14239	43178-43183	NNS	denotes	roots
T14242	43184-43185	-LRB-	denotes	(
T14243	43185-43187	NN	denotes	L3
T14244	43187-43188	-RRB-	denotes	)
T14245	43189-43191	IN	denotes	at
T14246	43192-43201	JJ	denotes	postnatal
T14247	43202-43205	NN	denotes	day
T14248	43206-43207	-LRB-	denotes	(
T14249	43207-43208	NN	denotes	P
T14250	43208-43209	-RRB-	denotes	)
T14251	43210-43211	CD	denotes	5
T14252	43212-43217	VBG	denotes	using
T14253	43218-43223	NN	denotes	glass
T14254	43224-43235	NNS	denotes	capillaries
T14255	43235-43236	.	denotes	.
T14256	43236-43313	sentence	denotes	After injection, animals were incubated for 2–3 h (E13.5) or overnight (P5).
T14257	43237-43242	IN	denotes	After
T14259	43243-43252	NN	denotes	injection
T14260	43252-43254	,	denotes	,
T14261	43254-43261	NNS	denotes	animals
T14262	43262-43266	VBD	denotes	were
T14258	43267-43276	VBN	denotes	incubated
T14263	43277-43280	IN	denotes	for
T14264	43281-43282	CD	denotes	2
T14266	43282-43283	SYM	denotes	–
T14265	43283-43284	CD	denotes	3
T14267	43285-43286	NN	denotes	h
T14268	43287-43288	-LRB-	denotes	(
T14269	43288-43293	NN	denotes	E13.5
T14270	43293-43294	-RRB-	denotes	)
T14271	43295-43297	CC	denotes	or
T14272	43298-43307	RB	denotes	overnight
T14273	43308-43309	-LRB-	denotes	(
T14274	43309-43311	NN	denotes	P5
T14275	43311-43312	-RRB-	denotes	)
T14276	43312-43313	.	denotes	.
T14277	43313-43468	sentence	denotes	Cryostat sections were processed for immunohistochemistry as described [14] using fluorophore-conjugated secondary antibodies (1:1,000, Molecular Probes).
T14278	43314-43322	NN	denotes	Cryostat
T14279	43323-43331	NNS	denotes	sections
T14281	43332-43336	VBD	denotes	were
T14280	43337-43346	VBN	denotes	processed
T14282	43347-43350	IN	denotes	for
T14283	43351-43371	NN	denotes	immunohistochemistry
T14284	43372-43374	IN	denotes	as
T14285	43375-43384	VBN	denotes	described
T14286	43385-43386	-LRB-	denotes	[
T14287	43386-43388	CD	denotes	14
T14288	43388-43389	-RRB-	denotes	]
T14289	43390-43395	VBG	denotes	using
T14290	43396-43407	NN	denotes	fluorophore
T14292	43407-43408	HYPH	denotes	-
T14291	43408-43418	VBN	denotes	conjugated
T14294	43419-43428	JJ	denotes	secondary
T14293	43429-43439	NNS	denotes	antibodies
T14295	43440-43441	-LRB-	denotes	(
T14297	43441-43442	CD	denotes	1
T14298	43442-43443	SYM	denotes	:
T14299	43443-43448	CD	denotes	1,000
T14300	43448-43450	,	denotes	,
T14301	43450-43459	NNP	denotes	Molecular
T14296	43460-43466	NNP	denotes	Probes
T14302	43466-43467	-RRB-	denotes	)
T14303	43467-43468	.	denotes	.
T14304	43468-43540	sentence	denotes	Images were collected on an Olympus (Tokyo, Japan) confocal microscope.
T14305	43469-43475	NNS	denotes	Images
T14307	43476-43480	VBD	denotes	were
T14306	43481-43490	VBN	denotes	collected
T14308	43491-43493	IN	denotes	on
T14309	43494-43496	DT	denotes	an
T14311	43497-43504	NNP	denotes	Olympus
T14312	43505-43506	-LRB-	denotes	(
T14313	43506-43511	NNP	denotes	Tokyo
T14314	43511-43513	,	denotes	,
T14315	43513-43518	NNP	denotes	Japan
T14316	43518-43519	-RRB-	denotes	)
T14317	43520-43528	JJ	denotes	confocal
T14310	43529-43539	NN	denotes	microscope
T14318	43539-43540	.	denotes	.
T14319	43540-43804	sentence	denotes	Images from in situ hybridization experiments were collected with an RT-SPOT camera (Diagnostic Instruments, Sterling Heights, Michigan, United States), and Corel (Eden Prairie, Minnesota, United States) Photo Paint 10.0 was used for digital processing of images.
T14320	43541-43547	NNS	denotes	Images
T14322	43548-43552	IN	denotes	from
T14323	43553-43555	FW	denotes	in
T14324	43556-43560	FW	denotes	situ
T14326	43561-43574	NN	denotes	hybridization
T14325	43575-43586	NNS	denotes	experiments
T14327	43587-43591	VBD	denotes	were
T14321	43592-43601	VBN	denotes	collected
T14328	43602-43606	IN	denotes	with
T14329	43607-43609	DT	denotes	an
T14331	43610-43612	NN	denotes	RT
T14333	43612-43613	HYPH	denotes	-
T14332	43613-43617	NN	denotes	SPOT
T14330	43618-43624	NN	denotes	camera
T14334	43625-43626	-LRB-	denotes	(
T14336	43626-43636	JJ	denotes	Diagnostic
T14335	43637-43648	NNPS	denotes	Instruments
T14337	43648-43650	,	denotes	,
T14338	43650-43658	NNP	denotes	Sterling
T14339	43659-43666	NNP	denotes	Heights
T14340	43666-43668	,	denotes	,
T14341	43668-43676	NNP	denotes	Michigan
T14342	43676-43678	,	denotes	,
T14343	43678-43684	NNP	denotes	United
T14344	43685-43691	NNP	denotes	States
T14345	43691-43692	-RRB-	denotes	)
T14346	43692-43694	,	denotes	,
T14347	43694-43697	CC	denotes	and
T14348	43698-43703	NNP	denotes	Corel
T14350	43704-43705	-LRB-	denotes	(
T14352	43705-43709	NNP	denotes	Eden
T14351	43710-43717	NNP	denotes	Prairie
T14353	43717-43719	,	denotes	,
T14354	43719-43728	NNP	denotes	Minnesota
T14355	43728-43730	,	denotes	,
T14356	43730-43736	NNP	denotes	United
T14357	43737-43743	NNP	denotes	States
T14358	43743-43744	-RRB-	denotes	)
T14359	43745-43750	NNP	denotes	Photo
T14349	43751-43756	NNP	denotes	Paint
T14361	43757-43761	CD	denotes	10.0
T14362	43762-43765	VBD	denotes	was
T14360	43766-43770	VBN	denotes	used
T14363	43771-43774	IN	denotes	for
T14364	43775-43782	JJ	denotes	digital
T14365	43783-43793	NN	denotes	processing
T14366	43794-43796	IN	denotes	of
T14367	43797-43803	NNS	denotes	images
T14368	43803-43804	.	denotes	.
T14469	43806-43808	FW	denotes	In
T14470	43809-43814	FW	denotes	vitro
T14471	43815-43823	NNS	denotes	cultures
T14472	43824-43826	IN	denotes	of
T14473	43827-43830	NN	denotes	DRG
T14474	43830-44242	sentence	denotes	Individual lumbar DRG were dissected from E13.5 or E14.5 embryos and placed on Matrigel (BD Biosciences, San Jose, California, United States) coated coverslips in DMEM/F12 (Gibco, San Diego, California, United States), 2 mM L-Gln (Gibco), N2 (Gibco), and 1 mg/ml BSA (Sigma, St. Louis, Missouri, United States) without neurotrophins, or supplemented with either NGF (100 ng/ml, Gibco) or NT-3 (20 ng/ml, Sigma).
T14475	43831-43841	JJ	denotes	Individual
T14477	43842-43848	NN	denotes	lumbar
T14476	43849-43852	NN	denotes	DRG
T14479	43853-43857	VBD	denotes	were
T14478	43858-43867	VBN	denotes	dissected
T14480	43868-43872	IN	denotes	from
T14481	43873-43878	NN	denotes	E13.5
T14483	43879-43881	CC	denotes	or
T14484	43882-43887	NN	denotes	E14.5
T14482	43888-43895	NNS	denotes	embryos
T14485	43896-43899	CC	denotes	and
T14486	43900-43906	VBN	denotes	placed
T14487	43907-43909	IN	denotes	on
T14488	43910-43918	NNP	denotes	Matrigel
T14490	43919-43920	-LRB-	denotes	(
T14492	43920-43922	NNP	denotes	BD
T14491	43923-43934	NNP	denotes	Biosciences
T14493	43934-43936	,	denotes	,
T14494	43936-43939	NNP	denotes	San
T14495	43940-43944	NNP	denotes	Jose
T14496	43944-43946	,	denotes	,
T14497	43946-43956	NNP	denotes	California
T14498	43956-43958	,	denotes	,
T14499	43958-43964	NNP	denotes	United
T14500	43965-43971	NNP	denotes	States
T14501	43971-43972	-RRB-	denotes	)
T14489	43973-43979	VBN	denotes	coated
T14502	43980-43990	NNS	denotes	coverslips
T14503	43991-43993	IN	denotes	in
T14504	43994-43998	NN	denotes	DMEM
T14506	43998-43999	HYPH	denotes	/
T14505	43999-44002	NN	denotes	F12
T14507	44003-44004	-LRB-	denotes	(
T14508	44004-44009	NNP	denotes	Gibco
T14509	44009-44011	,	denotes	,
T14510	44011-44014	NNP	denotes	San
T14511	44015-44020	NNP	denotes	Diego
T14512	44020-44022	,	denotes	,
T14513	44022-44032	NNP	denotes	California
T14514	44032-44034	,	denotes	,
T14515	44034-44040	NNP	denotes	United
T14516	44041-44047	NNP	denotes	States
T14517	44047-44048	-RRB-	denotes	)
T14518	44048-44050	,	denotes	,
T14519	44050-44051	CD	denotes	2
T14520	44052-44054	NN	denotes	mM
T14522	44055-44056	NN	denotes	L
T14523	44056-44057	HYPH	denotes	-
T14521	44057-44060	NN	denotes	Gln
T14524	44061-44062	-LRB-	denotes	(
T14525	44062-44067	NNP	denotes	Gibco
T14526	44067-44068	-RRB-	denotes	)
T14527	44068-44070	,	denotes	,
T14528	44070-44072	NN	denotes	N2
T14529	44073-44074	-LRB-	denotes	(
T14530	44074-44079	NNP	denotes	Gibco
T14531	44079-44080	-RRB-	denotes	)
T14532	44080-44082	,	denotes	,
T14533	44082-44085	CC	denotes	and
T14534	44086-44087	CD	denotes	1
T14535	44088-44090	NN	denotes	mg
T14537	44090-44091	SYM	denotes	/
T14538	44091-44093	NN	denotes	ml
T14536	44094-44097	NN	denotes	BSA
T14539	44098-44099	-LRB-	denotes	(
T14540	44099-44104	NNP	denotes	Sigma
T14541	44104-44106	,	denotes	,
T14542	44106-44109	NNP	denotes	St.
T14543	44110-44115	NNP	denotes	Louis
T14544	44115-44117	,	denotes	,
T14545	44117-44125	NNP	denotes	Missouri
T14546	44125-44127	,	denotes	,
T14547	44127-44133	NNP	denotes	United
T14548	44134-44140	NNP	denotes	States
T14549	44140-44141	-RRB-	denotes	)
T14550	44142-44149	IN	denotes	without
T14551	44150-44163	NNS	denotes	neurotrophins
T14552	44163-44165	,	denotes	,
T14553	44165-44167	CC	denotes	or
T14554	44168-44180	VBN	denotes	supplemented
T14555	44181-44185	IN	denotes	with
T14556	44186-44192	CC	denotes	either
T14557	44193-44196	NN	denotes	NGF
T14558	44197-44198	-LRB-	denotes	(
T14560	44198-44201	CD	denotes	100
T14561	44202-44204	NN	denotes	ng
T14562	44204-44205	SYM	denotes	/
T14563	44205-44207	NN	denotes	ml
T14564	44207-44209	,	denotes	,
T14559	44209-44214	NNP	denotes	Gibco
T14565	44214-44215	-RRB-	denotes	)
T14566	44216-44218	CC	denotes	or
T14567	44219-44221	NN	denotes	NT
T14568	44221-44222	HYPH	denotes	-
T14569	44222-44223	CD	denotes	3
T14570	44224-44225	-LRB-	denotes	(
T14572	44225-44227	CD	denotes	20
T14573	44228-44230	NN	denotes	ng
T14574	44230-44231	SYM	denotes	/
T14575	44231-44233	NN	denotes	ml
T14576	44233-44235	,	denotes	,
T14571	44235-44240	NNP	denotes	Sigma
T14577	44240-44241	-RRB-	denotes	)
T14578	44241-44242	.	denotes	.
T14579	44242-44382	sentence	denotes	DRG explants (n ≥ 20 for each condition) were cultured for 48 h, processed for immunocytochemistry, and analyzed using confocal microscopy.
T14580	44243-44246	NN	denotes	DRG
T14581	44247-44255	NNS	denotes	explants
T14583	44256-44257	-LRB-	denotes	(
T14585	44257-44258	NN	denotes	n
T14586	44259-44260	SYM	denotes	≥
T14584	44261-44263	CD	denotes	20
T14587	44264-44267	IN	denotes	for
T14588	44268-44272	DT	denotes	each
T14589	44273-44282	NN	denotes	condition
T14590	44282-44283	-RRB-	denotes	)
T14591	44284-44288	VBD	denotes	were
T14582	44289-44297	VBN	denotes	cultured
T14592	44298-44301	IN	denotes	for
T14593	44302-44304	CD	denotes	48
T14594	44305-44306	NN	denotes	h
T14595	44306-44308	,	denotes	,
T14596	44308-44317	VBN	denotes	processed
T14597	44318-44321	IN	denotes	for
T14598	44322-44341	NN	denotes	immunocytochemistry
T14599	44341-44343	,	denotes	,
T14600	44343-44346	CC	denotes	and
T14601	44347-44355	VBN	denotes	analyzed
T14602	44356-44361	VBG	denotes	using
T14603	44362-44370	JJ	denotes	confocal
T14604	44371-44381	NN	denotes	microscopy
T14605	44381-44382	.	denotes	.
T14754	44384-44391	NNP	denotes	Western
T14755	44392-44396	NN	denotes	blot
T14756	44397-44405	NN	denotes	analysis
T14757	44405-44621	sentence	denotes	Lumbar DRG from E16.5 embryos were isolated, mechanically disrupted, homogenized using glass beads (Sigma), and lysed in standard lysis buffer supplemented with protease and phosphatase inhibitors as described [47].
T14758	44406-44412	NN	denotes	Lumbar
T14759	44413-44416	NN	denotes	DRG
T14761	44417-44421	IN	denotes	from
T14762	44422-44427	NN	denotes	E16.5
T14763	44428-44435	NNS	denotes	embryos
T14764	44436-44440	VBD	denotes	were
T14760	44441-44449	VBN	denotes	isolated
T14765	44449-44451	,	denotes	,
T14766	44451-44463	RB	denotes	mechanically
T14767	44464-44473	VBN	denotes	disrupted
T14768	44473-44475	,	denotes	,
T14769	44475-44486	VBN	denotes	homogenized
T14770	44487-44492	VBG	denotes	using
T14771	44493-44498	NN	denotes	glass
T14772	44499-44504	NNS	denotes	beads
T14773	44505-44506	-LRB-	denotes	(
T14774	44506-44511	NNP	denotes	Sigma
T14775	44511-44512	-RRB-	denotes	)
T14776	44512-44514	,	denotes	,
T14777	44514-44517	CC	denotes	and
T14778	44518-44523	VBN	denotes	lysed
T14779	44524-44526	IN	denotes	in
T14780	44527-44535	JJ	denotes	standard
T14782	44536-44541	NN	denotes	lysis
T14781	44542-44548	NN	denotes	buffer
T14783	44549-44561	VBN	denotes	supplemented
T14784	44562-44566	IN	denotes	with
T14785	44567-44575	NN	denotes	protease
T14787	44576-44579	CC	denotes	and
T14788	44580-44591	NN	denotes	phosphatase
T14786	44592-44602	NNS	denotes	inhibitors
T14789	44603-44605	IN	denotes	as
T14790	44606-44615	VBN	denotes	described
T14791	44616-44617	-LRB-	denotes	[
T14792	44617-44619	CD	denotes	47
T14793	44619-44620	-RRB-	denotes	]
T14794	44620-44621	.	denotes	.
T14795	44621-44911	sentence	denotes	Protein extracts were resolved by SDS-PAGE, and immunoblotting was performed using antibodies against Akt, p-Akt (Ser473), CREB, p-CREB (Ser133), Bax, Bcl-xl (Cell Signaling Technology, Beverly, Massachusetts, United States), and Bcl2 (BD Pharmingen, San Diego, California, United States).
T14796	44622-44629	NN	denotes	Protein
T14797	44630-44638	NNS	denotes	extracts
T14799	44639-44643	VBD	denotes	were
T14798	44644-44652	VBN	denotes	resolved
T14800	44653-44655	IN	denotes	by
T14801	44656-44659	NN	denotes	SDS
T14803	44659-44660	HYPH	denotes	-
T14802	44660-44664	NN	denotes	PAGE
T14804	44664-44666	,	denotes	,
T14805	44666-44669	CC	denotes	and
T14806	44670-44684	NN	denotes	immunoblotting
T14808	44685-44688	VBD	denotes	was
T14807	44689-44698	VBN	denotes	performed
T14809	44699-44704	VBG	denotes	using
T14810	44705-44715	NNS	denotes	antibodies
T14811	44716-44723	IN	denotes	against
T14812	44724-44727	NN	denotes	Akt
T14813	44727-44729	,	denotes	,
T14814	44729-44730	NN	denotes	p
T14816	44730-44731	HYPH	denotes	-
T14815	44731-44734	NN	denotes	Akt
T14817	44735-44736	-LRB-	denotes	(
T14818	44736-44742	NN	denotes	Ser473
T14819	44742-44743	-RRB-	denotes	)
T14820	44743-44745	,	denotes	,
T14821	44745-44749	NN	denotes	CREB
T14822	44749-44751	,	denotes	,
T14823	44751-44752	NN	denotes	p
T14825	44752-44753	HYPH	denotes	-
T14824	44753-44757	NN	denotes	CREB
T14826	44758-44759	-LRB-	denotes	(
T14827	44759-44765	NN	denotes	Ser133
T14828	44765-44766	-RRB-	denotes	)
T14829	44766-44768	,	denotes	,
T14830	44768-44771	NN	denotes	Bax
T14831	44771-44773	,	denotes	,
T14832	44773-44776	NN	denotes	Bcl
T14834	44776-44777	HYPH	denotes	-
T14833	44777-44779	NN	denotes	xl
T14835	44780-44781	-LRB-	denotes	(
T14837	44781-44785	NNP	denotes	Cell
T14838	44786-44795	NNP	denotes	Signaling
T14836	44796-44806	NNP	denotes	Technology
T14839	44806-44808	,	denotes	,
T14840	44808-44815	NNP	denotes	Beverly
T14841	44815-44817	,	denotes	,
T14842	44817-44830	NNP	denotes	Massachusetts
T14843	44830-44832	,	denotes	,
T14844	44832-44838	NNP	denotes	United
T14845	44839-44845	NNP	denotes	States
T14846	44845-44846	-RRB-	denotes	)
T14847	44846-44848	,	denotes	,
T14848	44848-44851	CC	denotes	and
T14849	44852-44856	NN	denotes	Bcl2
T14850	44857-44858	-LRB-	denotes	(
T14852	44858-44860	NN	denotes	BD
T14851	44861-44871	NNP	denotes	Pharmingen
T14853	44871-44873	,	denotes	,
T14854	44873-44876	NNP	denotes	San
T14855	44877-44882	NNP	denotes	Diego
T14856	44882-44884	,	denotes	,
T14857	44884-44894	NNP	denotes	California
T14858	44894-44896	,	denotes	,
T14859	44896-44902	NNP	denotes	United
T14860	44903-44909	NNP	denotes	States
T14861	44909-44910	-RRB-	denotes	)
T14862	44910-44911	.	denotes	.
T14863	44911-45114	sentence	denotes	For quantification, films (X-OMAT AR, Eastman Kodak, Rochester, New York, United States) were scanned and densitometry was performed using IMAGEQUANT 5.2 (Molecular Dynamics, Amersham, Uppsala, Sweden).
T14864	44912-44915	IN	denotes	For
T14866	44916-44930	NN	denotes	quantification
T14867	44930-44932	,	denotes	,
T14868	44932-44937	NNS	denotes	films
T14869	44938-44939	-LRB-	denotes	(
T14871	44939-44940	NN	denotes	X
T14873	44940-44941	HYPH	denotes	-
T14872	44941-44945	NN	denotes	OMAT
T14870	44946-44948	NNP	denotes	AR
T14874	44948-44950	,	denotes	,
T14875	44950-44957	NNP	denotes	Eastman
T14876	44958-44963	NNP	denotes	Kodak
T14877	44963-44965	,	denotes	,
T14878	44965-44974	NNP	denotes	Rochester
T14879	44974-44976	,	denotes	,
T14880	44976-44979	NNP	denotes	New
T14881	44980-44984	NNP	denotes	York
T14882	44984-44986	,	denotes	,
T14883	44986-44992	NNP	denotes	United
T14884	44993-44999	NNP	denotes	States
T14885	44999-45000	-RRB-	denotes	)
T14886	45001-45005	VBD	denotes	were
T14865	45006-45013	VBN	denotes	scanned
T14887	45014-45017	CC	denotes	and
T14888	45018-45030	NN	denotes	densitometry
T14890	45031-45034	VBD	denotes	was
T14889	45035-45044	VBN	denotes	performed
T14891	45045-45050	VBG	denotes	using
T14892	45051-45061	NNP	denotes	IMAGEQUANT
T14893	45062-45065	CD	denotes	5.2
T14894	45066-45067	-LRB-	denotes	(
T14896	45067-45076	NNP	denotes	Molecular
T14895	45077-45085	NNP	denotes	Dynamics
T14897	45085-45087	,	denotes	,
T14898	45087-45095	NNP	denotes	Amersham
T14899	45095-45097	,	denotes	,
T14900	45097-45104	NNP	denotes	Uppsala
T14901	45104-45106	,	denotes	,
T14902	45106-45112	NNP	denotes	Sweden
T14903	45112-45113	-RRB-	denotes	)
T14904	45113-45114	.	denotes	.
T15020	45116-45133	NN	denotes	Electrophysiology
T15021	45133-45207	sentence	denotes	Electrophysiological analysis was performed as previously described [48].
T15022	45134-45154	JJ	denotes	Electrophysiological
T15023	45155-45163	NN	denotes	analysis
T15025	45164-45167	VBD	denotes	was
T15024	45168-45177	VBN	denotes	performed
T15026	45178-45180	IN	denotes	as
T15028	45181-45191	RB	denotes	previously
T15027	45192-45201	VBN	denotes	described
T15029	45202-45203	-LRB-	denotes	[
T15030	45203-45205	CD	denotes	48
T15031	45205-45206	-RRB-	denotes	]
T15032	45206-45207	.	denotes	.
T15033	45207-45336	sentence	denotes	Briefly, intracellular recordings from identified quadriceps motor neurons were made using sharp electrodes (75–120 MΩ, 3M KCl).
T15034	45208-45215	RB	denotes	Briefly
T15036	45215-45217	,	denotes	,
T15037	45217-45230	JJ	denotes	intracellular
T15038	45231-45241	NNS	denotes	recordings
T15039	45242-45246	IN	denotes	from
T15040	45247-45257	VBN	denotes	identified
T15042	45258-45268	NNS	denotes	quadriceps
T15043	45269-45274	NN	denotes	motor
T15041	45275-45282	NNS	denotes	neurons
T15044	45283-45287	VBD	denotes	were
T15035	45288-45292	VBN	denotes	made
T15045	45293-45298	VBG	denotes	using
T15046	45299-45304	JJ	denotes	sharp
T15047	45305-45315	NNS	denotes	electrodes
T15048	45316-45317	-LRB-	denotes	(
T15050	45317-45319	CD	denotes	75
T15052	45319-45320	SYM	denotes	–
T15051	45320-45323	CD	denotes	120
T15049	45324-45326	NN	denotes	MΩ
T15053	45326-45328	,	denotes	,
T15054	45328-45330	NN	denotes	3M
T15055	45331-45334	NN	denotes	KCl
T15056	45334-45335	-RRB-	denotes	)
T15057	45335-45336	.	denotes	.
T15058	45336-45536	sentence	denotes	Average responses (10–20 trials) from suprathreshold nerve stimulation (1.5 times the strength that evokes maximal monosynaptic response) of the quadriceps nerve were acquired with LTP software [49].
T15059	45337-45344	JJ	denotes	Average
T15060	45345-45354	NNS	denotes	responses
T15062	45355-45356	-LRB-	denotes	(
T15064	45356-45358	CD	denotes	10
T15066	45358-45359	SYM	denotes	–
T15065	45359-45361	CD	denotes	20
T15063	45362-45368	NNS	denotes	trials
T15067	45368-45369	-RRB-	denotes	)
T15068	45370-45374	IN	denotes	from
T15069	45375-45389	NN	denotes	suprathreshold
T15071	45390-45395	NN	denotes	nerve
T15070	45396-45407	NN	denotes	stimulation
T15072	45408-45409	-LRB-	denotes	(
T15074	45409-45412	CD	denotes	1.5
T15075	45413-45418	NNS	denotes	times
T15076	45419-45422	DT	denotes	the
T15073	45423-45431	NN	denotes	strength
T15077	45432-45436	WDT	denotes	that
T15078	45437-45443	VBZ	denotes	evokes
T15079	45444-45451	JJ	denotes	maximal
T15081	45452-45464	JJ	denotes	monosynaptic
T15080	45465-45473	NN	denotes	response
T15082	45473-45474	-RRB-	denotes	)
T15083	45475-45477	IN	denotes	of
T15084	45478-45481	DT	denotes	the
T15086	45482-45492	NNS	denotes	quadriceps
T15085	45493-45498	NN	denotes	nerve
T15087	45499-45503	VBD	denotes	were
T15061	45504-45512	VBN	denotes	acquired
T15088	45513-45517	IN	denotes	with
T15089	45518-45521	NN	denotes	LTP
T15090	45522-45530	NN	denotes	software
T15091	45531-45532	-LRB-	denotes	[
T15092	45532-45534	CD	denotes	49
T15093	45534-45535	-RRB-	denotes	]
T15094	45535-45536	.	denotes	.
T15095	45536-45634	sentence	denotes	Only cells with stable resting potentials more negative than −50 mV were considered for analysis.
T15096	45537-45541	RB	denotes	Only
T15097	45542-45547	NNS	denotes	cells
T15099	45548-45552	IN	denotes	with
T15100	45553-45559	JJ	denotes	stable
T15102	45560-45567	NN	denotes	resting
T15101	45568-45578	NNS	denotes	potentials
T15103	45579-45583	RBR	denotes	more
T15104	45584-45592	JJ	denotes	negative
T15105	45593-45597	IN	denotes	than
T15106	45598-45599	SYM	denotes	−
T15107	45599-45601	CD	denotes	50
T15108	45602-45604	NN	denotes	mV
T15109	45605-45609	VBD	denotes	were
T15098	45610-45620	VBN	denotes	considered
T15110	45621-45624	IN	denotes	for
T15111	45625-45633	NN	denotes	analysis
T15112	45633-45634	.	denotes	.
T15113	45634-45814	sentence	denotes	Monosynaptic amplitudes were determined offline using custom routines in the Matlab environment (The Mathworks, Natick, Massachusetts, United States) as previously described [48].
T15114	45635-45647	JJ	denotes	Monosynaptic
T15115	45648-45658	NNS	denotes	amplitudes
T15117	45659-45663	VBD	denotes	were
T15116	45664-45674	VBN	denotes	determined
T15118	45675-45682	RB	denotes	offline
T15119	45683-45688	VBG	denotes	using
T15120	45689-45695	NN	denotes	custom
T15121	45696-45704	NNS	denotes	routines
T15122	45705-45707	IN	denotes	in
T15123	45708-45711	DT	denotes	the
T15125	45712-45718	NNP	denotes	Matlab
T15124	45719-45730	NN	denotes	environment
T15126	45731-45732	-LRB-	denotes	(
T15128	45732-45735	DT	denotes	The
T15127	45736-45745	NNP	denotes	Mathworks
T15129	45745-45747	,	denotes	,
T15130	45747-45753	NNP	denotes	Natick
T15131	45753-45755	,	denotes	,
T15132	45755-45768	NNP	denotes	Massachusetts
T15133	45768-45770	,	denotes	,
T15134	45770-45776	NNP	denotes	United
T15135	45777-45783	NNP	denotes	States
T15136	45783-45784	-RRB-	denotes	)
T15137	45785-45787	IN	denotes	as
T15139	45788-45798	RB	denotes	previously
T15138	45799-45808	VBN	denotes	described
T15140	45809-45810	-LRB-	denotes	[
T15141	45810-45812	CD	denotes	48
T15142	45812-45813	-RRB-	denotes	]
T15143	45813-45814	.	denotes	.
R1000	T3610	T3612	nsubj	Pea3,Replace
R1001	T3611	T3610	punct	-,Pea3
R1002	T3613	T3612	aux	Can,Replace
R1003	T3614	T3615	compound	Er81,Function
R1004	T3615	T3612	dobj	Function,Replace
R1005	T3616	T3612	prep	in,Replace
R1006	T3617	T3616	pcomp	Controlling,in
R1007	T3618	T3619	nmod	Ia,Projections
R1008	T3619	T3617	dobj	Projections,Controlling
R1009	T3620	T3619	amod	Afferent,Projections
R1010	T3622	T3623	nsubj	We,defined
R1011	T3624	T3623	advmod	first,defined
R1012	T3625	T3626	det	an,regulator
R1013	T3626	T3623	dobj	regulator,defined
R1014	T3627	T3626	compound	ETS,regulator
R1015	T3628	T3626	compound	transcription,regulator
R1016	T3629	T3630	dep	that,is
R1017	T3630	T3626	relcl	is,regulator
R1018	T3631	T3630	acomp	able,is
R1019	T3632	T3633	aux	to,replace
R1020	T3633	T3631	xcomp	replace,able
R1021	T3634	T3635	det	the,function
R1022	T3635	T3633	dobj	function,replace
R1023	T3636	T3635	prep	of,function
R1024	T3637	T3636	pobj	Er81,of
R1025	T3638	T3635	prep	within,function
R1026	T3639	T3640	amod	proprioceptive,afferents
R1027	T3640	T3638	pobj	afferents,within
R1028	T3641	T3642	aux	to,direct
R1029	T3642	T3633	advcl	direct,replace
R1030	T3643	T3642	dobj	projections,direct
R1031	T3644	T3642	prep	into,direct
R1032	T3645	T3646	det	the,cord
R1033	T3646	T3644	pobj	cord,into
R1034	T3647	T3646	amod	ventral,cord
R1035	T3648	T3646	amod	spinal,cord
R1036	T3649	T3623	punct	.,defined
R1037	T3651	T3652	nsubj	Er81,constitute
R1038	T3653	T3651	punct	", ",Er81
R1039	T3654	T3651	conj	Pea3,Er81
R1040	T3655	T3654	punct	", ",Pea3
R1041	T3656	T3654	cc	and,Pea3
R1042	T3657	T3654	conj	Erm,Pea3
R1043	T3658	T3659	det	the,subfamily
R1044	T3659	T3652	dobj	subfamily,constitute
R1045	T3660	T3659	compound	Pea3,subfamily
R1046	T3661	T3659	prep	of,subfamily
R1047	T3662	T3663	compound	ETS,factors
R1048	T3663	T3661	pobj	factors,of
R1049	T3664	T3663	compound	transcription,factors
R1050	T3665	T3652	punct	", ",constitute
R1051	T3666	T3652	conj	show,constitute
R1052	T3667	T3668	det	a,degree
R1053	T3668	T3666	dobj	degree,show
R1054	T3669	T3668	amod	high,degree
R1055	T3670	T3668	prep	of,degree
R1056	T3671	T3672	compound	amino,acid
R1057	T3672	T3673	compound	acid,identity
R1058	T3673	T3670	pobj	identity,of
R1059	T3674	T3666	punct	", ",show
R1060	T3675	T3666	cc	and,show
R1061	T3676	T3666	conj	bind,show
R1062	T3677	T3676	prep	to,bind
R1063	T3678	T3679	advmod	very,similar
R1064	T3679	T3680	amod	similar,sequences
R1065	T3680	T3677	pobj	sequences,to
R1066	T3681	T3680	compound	DNA,sequences
R1067	T3682	T3680	compound	target,sequences
R1068	T3683	T3684	punct	[,19
R1069	T3684	T3676	parataxis	19,bind
R1070	T3685	T3684	nummod	17,19
R1071	T3686	T3684	punct	",",19
R1072	T3687	T3684	nummod	18,19
R1073	T3688	T3684	punct	",",19
R1074	T3689	T3684	punct	],19
R1075	T3690	T3652	punct	.,constitute
R1076	T3692	T3693	advmod	Nevertheless,rescue
R1077	T3694	T3693	punct	", ",rescue
R1078	T3695	T3696	advmod	when,introduced
R1079	T3696	T3693	advcl	introduced,rescue
R1080	T3697	T3696	prep	into,introduced
R1081	T3698	T3699	det	the,locus
R1082	T3699	T3697	pobj	locus,into
R1083	T3700	T3699	compound	Er81,locus
R1084	T3701	T3696	prep	in,introduced
R1085	T3702	T3701	pobj	analogy,in
R1086	T3703	T3702	prep	to,analogy
R1087	T3704	T3705	det	a,strategy
R1088	T3705	T3703	pobj	strategy,to
R1089	T3706	T3707	advmod	previously,used
R1090	T3707	T3705	amod	used,strategy
R1091	T3708	T3705	compound	targeting,strategy
R1092	T3709	T3710	punct	(,shown
R1093	T3710	T3696	parataxis	shown,introduced
R1094	T3711	T3710	nsubj	data,shown
R1095	T3712	T3710	neg	not,shown
R1096	T3713	T3710	punct	;,shown
R1097	T3714	T3715	punct	[,14
R1098	T3715	T3710	parataxis	14,shown
R1099	T3716	T3715	punct	],14
R1100	T3717	T3710	punct	),shown
R1101	T3718	T3693	punct	", ",rescue
R1102	T3719	T3720	preconj	neither,Pea3
R1103	T3720	T3693	nsubj	Pea3,rescue
R1104	T3721	T3720	cc	nor,Pea3
R1105	T3722	T3720	conj	Erm,Pea3
R1106	T3723	T3693	aux	could,rescue
R1107	T3724	T3725	npadvmod	Ia,proprioceptive
R1108	T3725	T3726	amod	proprioceptive,projections
R1109	T3726	T3693	dobj	projections,rescue
R1110	T3727	T3726	amod	afferent,projections
R1111	T3728	T3729	aux	to,invade
R1112	T3729	T3693	advcl	invade,rescue
R1113	T3730	T3729	advmod	extensively,invade
R1114	T3731	T3732	det	the,horn
R1115	T3732	T3729	dobj	horn,invade
R1116	T3733	T3732	amod	ventral,horn
R1117	T3734	T3732	prep	of,horn
R1118	T3735	T3736	det	the,cord
R1119	T3736	T3734	pobj	cord,of
R1120	T3737	T3736	amod	spinal,cord
R1121	T3738	T3739	punct	(,shown
R1122	T3739	T3729	parataxis	shown,invade
R1123	T3740	T3739	nsubj	data,shown
R1124	T3741	T3739	neg	not,shown
R1125	T3742	T3739	punct	),shown
R1126	T3743	T3693	punct	.,rescue
R1127	T3745	T3746	det	These,findings
R1128	T3746	T3747	nsubj	findings,prompted
R1129	T3748	T3747	dobj	us,prompted
R1130	T3749	T3750	aux	to,analyze
R1131	T3750	T3747	xcomp	analyze,prompted
R1132	T3751	T3750	dobj	mice,analyze
R1133	T3752	T3753	prep	in,integrated
R1134	T3753	T3751	relcl	integrated,mice
R1135	T3754	T3752	pobj	which,in
R1136	T3755	T3753	nsubj	we,integrated
R1137	T3756	T3757	compound	EWS,Pea3
R1138	T3757	T3753	dobj	Pea3,integrated
R1139	T3758	T3757	punct	-,Pea3
R1140	T3759	T3757	punct	", ",Pea3
R1141	T3760	T3761	det	a,product
R1142	T3761	T3757	appos	product,Pea3
R1143	T3762	T3763	compound	break,point
R1144	T3763	T3761	compound	point,product
R1145	T3764	T3763	punct	-,point
R1146	T3765	T3761	compound	fusion,product
R1147	T3766	T3761	prep	between,product
R1148	T3767	T3768	det	the,domain
R1149	T3768	T3766	pobj	domain,between
R1150	T3769	T3770	npadvmod	amino,terminal
R1151	T3770	T3768	amod	terminal,domain
R1152	T3771	T3770	punct	-,terminal
R1153	T3772	T3768	prep	of,domain
R1154	T3773	T3774	det	the,gene
R1155	T3774	T3772	pobj	gene,of
R1156	T3775	T3776	nmod	Ewing,sarcoma
R1157	T3776	T3774	nmod	sarcoma,gene
R1158	T3777	T3776	punct	(,sarcoma
R1159	T3778	T3776	appos	EWS,sarcoma
R1160	T3779	T3774	punct	),gene
R1161	T3780	T3768	cc	and,domain
R1162	T3781	T3782	det	the,domain
R1163	T3782	T3768	conj	domain,domain
R1164	T3783	T3784	compound	Pea3,DNA
R1165	T3784	T3785	npadvmod	DNA,binding
R1166	T3785	T3782	amod	binding,domain
R1167	T3786	T3787	punct	[,21
R1168	T3787	T3782	parataxis	21,domain
R1169	T3788	T3787	nummod	20,21
R1170	T3789	T3787	punct	",",21
R1171	T3790	T3787	punct	],21
R1172	T3791	T3753	punct	", ",integrated
R1173	T3792	T3753	prep	into,integrated
R1174	T3793	T3794	det	the,locus
R1175	T3794	T3792	pobj	locus,into
R1176	T3795	T3794	compound	Er81,locus
R1177	T3796	T3797	punct	(,Figure
R1178	T3797	T3753	parataxis	Figure,integrated
R1179	T3798	T3797	nummod	1,Figure
R1180	T3799	T3797	punct	),Figure
R1181	T3800	T3747	punct	.,prompted
R1182	T3802	T3803	nsubj	We,found
R1183	T3804	T3805	mark	that,showed
R1184	T3805	T3803	ccomp	showed,found
R1185	T3806	T3805	prep	in,showed
R1186	T3807	T3808	det	a,assay
R1187	T3808	T3806	pobj	assay,in
R1188	T3809	T3810	compound	luciferase,enzyme
R1189	T3810	T3812	npadvmod	enzyme,based
R1190	T3811	T3810	punct	-,enzyme
R1191	T3812	T3808	amod	based,assay
R1192	T3813	T3812	punct	-,based
R1193	T3814	T3815	compound	cell,culture
R1194	T3815	T3808	compound	culture,assay
R1195	T3816	T3808	compound	transfection,assay
R1196	T3817	T3805	punct	", ",showed
R1197	T3818	T3819	compound	EWS,Pea3
R1198	T3819	T3805	nsubj	Pea3,showed
R1199	T3820	T3819	punct	-,Pea3
R1200	T3821	T3822	amod	stronger,activity
R1201	T3822	T3805	dobj	activity,showed
R1202	T3823	T3822	compound	transactivation,activity
R1203	T3824	T3822	prep	than,activity
R1204	T3825	T3824	pobj	Er81,than
R1205	T3826	T3825	cc	or,Er81
R1206	T3827	T3825	conj	Pea3,Er81
R1207	T3828	T3829	punct	(,1J
R1208	T3829	T3822	parataxis	1J,activity
R1209	T3830	T3829	compound	Figure,1J
R1210	T3831	T3829	punct	;,1J
R1211	T3832	T3833	nsubj	data,shown
R1212	T3833	T3829	ccomp	shown,1J
R1213	T3834	T3833	neg	not,shown
R1214	T3835	T3829	punct	),1J
R1215	T3836	T3805	punct	", ",showed
R1216	T3837	T3805	prep	in,showed
R1217	T3838	T3837	pobj	agreement,in
R1218	T3839	T3838	prep	with,agreement
R1219	T3840	T3841	amod	previous,studies
R1220	T3841	T3839	pobj	studies,with
R1221	T3842	T3843	punct	[,24
R1222	T3843	T3841	parataxis	24,studies
R1223	T3844	T3843	nummod	22,24
R1224	T3845	T3843	punct	",",24
R1225	T3846	T3843	nummod	23,24
R1226	T3847	T3843	punct	",",24
R1227	T3848	T3843	punct	],24
R1228	T3849	T3803	punct	.,found
R1229	T3851	T3852	advmod	Moreover,abolished
R1230	T3853	T3852	punct	", ",abolished
R1231	T3854	T3852	nsubjpass	transactivation,abolished
R1232	T3855	T3854	prep	by,transactivation
R1233	T3856	T3857	compound	EWS,Pea3
R1234	T3857	T3855	pobj	Pea3,by
R1235	T3858	T3857	punct	-,Pea3
R1236	T3859	T3852	auxpass	was,abolished
R1237	T3860	T3852	agent	by,abolished
R1238	T3861	T3860	pobj	mutation,by
R1239	T3862	T3861	prep	of,mutation
R1240	T3863	T3864	npadvmod	ETS,binding
R1241	T3864	T3866	amod	binding,sites
R1242	T3865	T3864	punct	-,binding
R1243	T3866	T3862	pobj	sites,of
R1244	T3867	T3861	prep	in,mutation
R1245	T3868	T3869	det	the,plasmid
R1246	T3869	T3867	pobj	plasmid,in
R1247	T3870	T3869	compound	reporter,plasmid
R1248	T3871	T3852	punct	", ",abolished
R1249	T3872	T3852	advcl	demonstrating,abolished
R1250	T3873	T3874	npadvmod	ETS,binding
R1251	T3874	T3876	amod	binding,site
R1252	T3875	T3874	punct	-,binding
R1253	T3876	T3878	compound	site,dependence
R1254	T3877	T3876	punct	-,site
R1255	T3878	T3872	dobj	dependence,demonstrating
R1256	T3879	T3880	punct	(,shown
R1257	T3880	T3872	parataxis	shown,demonstrating
R1258	T3881	T3880	nsubj	data,shown
R1259	T3882	T3880	neg	not,shown
R1260	T3883	T3880	punct	),shown
R1261	T3884	T3852	punct	.,abolished
R1262	T3886	T3887	nsubjpass	Expression,abolished
R1263	T3888	T3886	prep	of,Expression
R1264	T3889	T3888	pobj	Er81,of
R1265	T3890	T3886	prep	in,Expression
R1266	T3891	T3892	compound	DRG,neurons
R1267	T3892	T3890	pobj	neurons,in
R1268	T3893	T3892	prep	of,neurons
R1269	T3894	T3893	pobj	embryos,of
R1270	T3895	T3894	acl	containing,embryos
R1271	T3896	T3895	dobj	integration,containing
R1272	T3897	T3896	prep	of,integration
R1273	T3898	T3899	compound	EWS,Pea3
R1274	T3899	T3897	pobj	Pea3,of
R1275	T3900	T3899	punct	-,Pea3
R1276	T3901	T3886	prep	in,Expression
R1277	T3902	T3903	det	the,locus
R1278	T3903	T3901	pobj	locus,in
R1279	T3904	T3903	compound	Er81,locus
R1280	T3905	T3903	punct	(,locus
R1281	T3906	T3907	compound	Er81EWS,Pea3
R1282	T3907	T3903	appos	Pea3,locus
R1283	T3908	T3907	punct	-,Pea3
R1284	T3909	T3907	punct	/,Pea3
R1285	T3910	T3907	punct	−,Pea3
R1286	T3911	T3887	punct	),abolished
R1287	T3912	T3887	auxpass	was,abolished
R1288	T3913	T3914	punct	(,1E
R1289	T3914	T3887	parataxis	1E,abolished
R1290	T3915	T3914	compound	Figure,1E
R1291	T3916	T3914	punct	),1E
R1292	T3917	T3887	punct	", ",abolished
R1293	T3918	T3887	cc	and,abolished
R1294	T3919	T3920	det	the,level
R1295	T3920	T3922	nsubj	level,was
R1296	T3921	T3920	compound	expression,level
R1297	T3922	T3887	conj	was,abolished
R1298	T3923	T3920	prep	of,level
R1299	T3924	T3925	det	the,protein
R1300	T3925	T3923	pobj	protein,of
R1301	T3926	T3927	npadvmod	calcium,binding
R1302	T3927	T3925	amod	binding,protein
R1303	T3928	T3927	punct	-,binding
R1304	T3929	T3925	appos	Parvalbumin,protein
R1305	T3930	T3929	punct	(,Parvalbumin
R1306	T3931	T3929	appos	PV,Parvalbumin
R1307	T3932	T3920	punct	),level
R1308	T3933	T3920	prep	in,level
R1309	T3934	T3935	amod	proprioceptive,afferents
R1310	T3935	T3933	pobj	afferents,in
R1311	T3936	T3920	punct	", ",level
R1312	T3937	T3938	dep	which,decreased
R1313	T3938	T3920	relcl	decreased,level
R1314	T3939	T3938	auxpass	is,decreased
R1315	T3940	T3941	advmod	approximately,10
R1316	T3941	T3945	quantmod	10,fold
R1317	T3942	T3941	quantmod	5,10
R1318	T3943	T3941	punct	-,10
R1319	T3944	T3941	quantmod	to,10
R1320	T3945	T3938	advmod	fold,decreased
R1321	T3946	T3945	punct	-,fold
R1322	T3947	T3938	prep	in,decreased
R1323	T3948	T3949	compound	Er81,mutants
R1324	T3949	T3947	pobj	mutants,in
R1325	T3950	T3951	punct	[,14
R1326	T3951	T3938	parataxis	14,decreased
R1327	T3952	T3951	punct	],14
R1328	T3953	T3922	punct	", ",was
R1329	T3954	T3922	acomp	comparable,was
R1330	T3955	T3954	prep	to,comparable
R1331	T3956	T3957	amod	wild,type
R1332	T3957	T3959	compound	type,levels
R1333	T3958	T3957	punct	-,type
R1334	T3959	T3955	pobj	levels,to
R1335	T3960	T3922	prep	in,was
R1336	T3961	T3962	nmod	Er81EWS,Pea3
R1337	T3962	T3964	nmod	Pea3,embryos
R1338	T3963	T3962	punct	-,Pea3
R1339	T3964	T3960	pobj	embryos,in
R1340	T3965	T3962	punct	/,Pea3
R1341	T3966	T3962	punct	−,Pea3
R1342	T3967	T3968	punct	(,1F
R1343	T3968	T3922	parataxis	1F,was
R1344	T3969	T3968	compound	Figure,1F
R1345	T3970	T3971	punct	–,1H
R1346	T3971	T3968	prep	1H,1F
R1347	T3972	T3968	punct	),1F
R1348	T3973	T3922	punct	.,was
R1349	T3975	T3976	aux	To,define
R1350	T3976	T3978	advcl	define,assessed
R1351	T3977	T3976	advmod	further,define
R1352	T3979	T3980	compound	DRG,neuron
R1353	T3980	T3981	compound	neuron,differentiation
R1354	T3981	T3976	dobj	differentiation,define
R1355	T3982	T3976	prep	in,define
R1356	T3983	T3984	det	the,presence
R1357	T3984	T3982	pobj	presence,in
R1358	T3985	T3984	prep	of,presence
R1359	T3986	T3987	compound	EWS,Pea3
R1360	T3987	T3985	pobj	Pea3,of
R1361	T3988	T3987	punct	-,Pea3
R1362	T3989	T3976	prep	in,define
R1363	T3990	T3991	amod	proprioceptive,afferents
R1364	T3991	T3989	pobj	afferents,in
R1365	T3992	T3993	advmod	in,vivo
R1366	T3993	T3976	advmod	vivo,define
R1367	T3994	T3978	punct	", ",assessed
R1368	T3995	T3978	nsubj	we,assessed
R1369	T3996	T3997	mark	whether,had
R1370	T3997	T3978	ccomp	had,assessed
R1371	T3998	T3997	nsubj	replacement,had
R1372	T3999	T3998	prep	of,replacement
R1373	T4000	T3999	pobj	Er81,of
R1374	T4001	T3998	prep	by,replacement
R1375	T4002	T4003	compound	EWS,Pea3
R1376	T4003	T4001	pobj	Pea3,by
R1377	T4004	T4003	punct	-,Pea3
R1378	T4005	T4006	det	an,influence
R1379	T4006	T3997	dobj	influence,had
R1380	T4007	T4006	prep	on,influence
R1381	T4008	T4009	amod	neuronal,survival
R1382	T4009	T4007	pobj	survival,on
R1383	T4010	T4007	cc	or,on
R1384	T4011	T4007	conj	on,on
R1385	T4012	T4013	det	the,expression
R1386	T4013	T4011	pobj	expression,on
R1387	T4014	T4013	prep	of,expression
R1388	T4015	T4016	amod	proprioceptive,afferent
R1389	T4016	T4018	amod	afferent,specific
R1390	T4017	T4016	punct	-,afferent
R1391	T4018	T4020	amod	specific,genes
R1392	T4019	T4018	punct	-,specific
R1393	T4020	T4014	pobj	genes,of
R1394	T4021	T3978	punct	.,assessed
R1395	T4023	T4024	nmod	Er81EWS,Pea3
R1396	T4024	T4026	nmod	Pea3,mice
R1397	T4025	T4024	punct	-,Pea3
R1398	T4026	T4029	nsubj	mice,differ
R1399	T4027	T4024	punct	/,Pea3
R1400	T4028	T4024	punct	−,Pea3
R1401	T4030	T4029	aux	did,differ
R1402	T4031	T4029	neg	not,differ
R1403	T4032	T4029	prep	from,differ
R1404	T4033	T4034	amod	wild,type
R1405	T4034	T4032	pobj	type,from
R1406	T4035	T4034	punct	-,type
R1407	T4036	T4029	prep	in,differ
R1408	T4037	T4038	det	the,number
R1409	T4038	T4036	pobj	number,in
R1410	T4039	T4038	prep	of,number
R1411	T4040	T4041	amod	proprioceptive,bodies
R1412	T4041	T4039	pobj	bodies,of
R1413	T4042	T4041	amod	afferent,bodies
R1414	T4043	T4041	compound	cell,bodies
R1415	T4044	T4041	prep	within,bodies
R1416	T4045	T4046	det	the,DRG
R1417	T4046	T4044	pobj	DRG,within
R1418	T4047	T4046	prep	of,DRG
R1419	T4048	T4047	pobj	L1,of
R1420	T4049	T4048	prep	to,L1
R1421	T4050	T4049	pobj	L5,to
R1422	T4051	T4038	punct	", ",number
R1423	T4052	T4053	det	the,expression
R1424	T4053	T4038	conj	expression,number
R1425	T4054	T4053	prep	of,expression
R1426	T4055	T4056	amod	several,genes
R1427	T4056	T4054	pobj	genes,of
R1428	T4057	T4058	advmod	normally,expressed
R1429	T4058	T4053	acl	expressed,expression
R1430	T4059	T4058	agent	by,expressed
R1431	T4060	T4061	amod	proprioceptive,afferents
R1432	T4061	T4059	pobj	afferents,by
R1433	T4062	T4053	punct	", ",expression
R1434	T4063	T4053	cc	and,expression
R1435	T4064	T4065	det	the,lack
R1436	T4065	T4053	conj	lack,expression
R1437	T4066	T4065	prep	of,lack
R1438	T4067	T4066	pobj	expression,of
R1439	T4068	T4067	prep	of,expression
R1440	T4069	T4068	pobj	genes,of
R1441	T4070	T4071	neg	not,expressed
R1442	T4071	T4069	acl	expressed,genes
R1443	T4072	T4071	advmod	normally,expressed
R1444	T4073	T4071	prep	in,expressed
R1445	T4074	T4075	amod	proprioceptive,afferents
R1446	T4075	T4073	pobj	afferents,in
R1447	T4076	T4077	punct	(,S1
R1448	T4077	T4029	parataxis	S1,differ
R1449	T4078	T4077	compound	Figure,S1
R1450	T4079	T4077	punct	;,S1
R1451	T4080	T4081	nsubj	data,shown
R1452	T4081	T4077	ccomp	shown,S1
R1453	T4082	T4081	neg	not,shown
R1454	T4083	T4077	punct	),S1
R1455	T4084	T4029	punct	.,differ
R1456	T4086	T4087	advmod	Together,suggest
R1457	T4088	T4087	punct	", ",suggest
R1458	T4089	T4090	det	these,findings
R1459	T4090	T4087	nsubj	findings,suggest
R1460	T4091	T4092	mark	that,mimics
R1461	T4092	T4087	ccomp	mimics,suggest
R1462	T4093	T4094	det	the,expression
R1463	T4094	T4092	nsubj	expression,mimics
R1464	T4095	T4094	prep	of,expression
R1465	T4096	T4097	compound	EWS,Pea3
R1466	T4097	T4095	pobj	Pea3,of
R1467	T4098	T4097	punct	-,Pea3
R1468	T4099	T4094	prep	from,expression
R1469	T4100	T4101	det	the,time
R1470	T4101	T4099	pobj	time,from
R1471	T4102	T4101	amod	normal,time
R1472	T4103	T4101	prep	of,time
R1473	T4104	T4103	pobj	onset,of
R1474	T4105	T4106	det	the,function
R1475	T4106	T4092	dobj	function,mimics
R1476	T4107	T4106	prep	of,function
R1477	T4108	T4107	pobj	Er81,of
R1478	T4109	T4110	mark	as,assessed
R1479	T4110	T4092	advcl	assessed,mimics
R1480	T4111	T4110	prep	by,assessed
R1481	T4112	T4111	pobj	induction,by
R1482	T4113	T4112	cc	and,induction
R1483	T4114	T4112	conj	maintenance,induction
R1484	T4115	T4112	prep	of,induction
R1485	T4116	T4117	compound	gene,expression
R1486	T4117	T4115	pobj	expression,of
R1487	T4118	T4112	prep	within,induction
R1488	T4119	T4120	amod	proprioceptive,afferents
R1489	T4120	T4118	pobj	afferents,within
R1490	T4121	T4087	punct	.,suggest
R1491	T4123	T4124	aux	To,determine
R1492	T4124	T4125	advcl	determine,traced
R1493	T4126	T4127	det	the,extent
R1494	T4127	T4124	dobj	extent,determine
R1495	T4128	T4127	prep	of,extent
R1496	T4129	T4128	pobj	rescue,of
R1497	T4130	T4129	prep	of,rescue
R1498	T4131	T4132	nmod	Ia,projections
R1499	T4132	T4130	pobj	projections,of
R1500	T4133	T4132	amod	proprioceptive,projections
R1501	T4134	T4132	amod	afferent,projections
R1502	T4135	T4132	prep	into,projections
R1503	T4136	T4137	det	the,cord
R1504	T4137	T4135	pobj	cord,into
R1505	T4138	T4137	amod	ventral,cord
R1506	T4139	T4137	amod	spinal,cord
R1507	T4140	T4137	prep	of,cord
R1508	T4141	T4142	compound	Er81,mice
R1509	T4142	T4140	pobj	mice,of
R1510	T4143	T4142	compound	mutant,mice
R1511	T4144	T4132	acl	achieved,projections
R1512	T4145	T4144	agent	by,achieved
R1513	T4146	T4145	pobj	expression,by
R1514	T4147	T4146	prep	of,expression
R1515	T4148	T4149	compound	EWS,Pea3
R1516	T4149	T4147	pobj	Pea3,of
R1517	T4150	T4149	punct	-,Pea3
R1518	T4151	T4125	punct	", ",traced
R1519	T4152	T4125	nsubj	we,traced
R1520	T4153	T4154	amod	intraspinal,projections
R1521	T4154	T4125	dobj	projections,traced
R1522	T4155	T4154	amod	afferent,projections
R1523	T4156	T4125	prep	by,traced
R1524	T4157	T4158	amod	axonal,labeling
R1525	T4158	T4156	pobj	labeling,by
R1526	T4265	T4266	nmod	vGlut1,terminals
R1527	T4266	T4246	dobj	terminals,formed
R1528	T4267	T4265	punct	+,vGlut1
R1529	T4268	T4269	dep	that,were
R1530	T4159	T4158	prep	of,labeling
R1531	T4269	T4266	relcl	were,terminals
R1532	T4160	T4159	pobj	PV,of
R1533	T4270	T4269	acomp	absent,were
R1534	T4161	T4162	punct	(,2A
R1535	T4162	T4125	parataxis	2A,traced
R1536	T4271	T4269	prep	in,were
R1537	T4163	T4162	compound	Figure,2A
R1538	T4164	T4165	punct	–,2C
R1539	T4165	T4162	prep	2C,2A
R1540	T4272	T4273	compound	Er81,mice
R1541	T4166	T4162	punct	),2A
R1542	T4167	T4125	punct	.,traced
R1543	T4273	T4271	pobj	mice,in
R1544	T4169	T4170	prep	In,used
R1545	T4274	T4273	compound	mutant,mice
R1546	T4171	T4169	pobj	addition,In
R1547	T4172	T4170	punct	", ",used
R1548	T4275	T4276	punct	(,2G
R1549	T4173	T4174	aux	to,analyze
R1550	T4174	T4170	advcl	analyze,used
R1551	T4276	T4269	parataxis	2G,were
R1552	T4175	T4176	compound	axon,ingrowth
R1553	T4176	T4174	dobj	ingrowth,analyze
R1554	T4177	T4176	amod	independent,ingrowth
R1555	T4277	T4276	compound	Figure,2G
R1556	T4178	T4177	prep	of,independent
R1557	T4179	T4180	det	the,level
R1558	T4180	T4178	pobj	level,of
R1559	T4278	T4279	punct	–,2I
R156	T1510	T1511	amod	Neuronal,differentiation
R1560	T4181	T4180	prep	of,level
R1561	T4182	T4183	compound	PV,expression
R1562	T4279	T4276	prep	2I,2G
R1563	T4183	T4181	pobj	expression,of
R1564	T4184	T4180	prep	in,level
R1565	T4185	T4186	compound	DRG,neurons
R1566	T4186	T4184	pobj	neurons,in
R1567	T4187	T4170	punct	", ",used
R1568	T4280	T4276	punct	),2G
R1569	T4188	T4170	nsubj	we,used
R157	T1511	T1512	nsubj	differentiation,is
R1570	T4189	T4190	amod	anterograde,labeling
R1571	T4190	T4170	dobj	labeling,used
R1572	T4281	T4246	punct	.,formed
R1573	T4191	T4190	prep	of,labeling
R1574	T4192	T4193	amod	afferent,fibers
R1575	T4283	T4284	aux	To,assess
R1576	T4193	T4191	pobj	fibers,of
R1577	T4284	T4285	advcl	assess,performed
R1578	T4194	T4170	prep	by,used
R1579	T4286	T4287	mark	whether,are
R158	T1513	T1514	det	a,process
R1580	T4195	T4194	pcomp	applying,by
R1581	T4196	T4197	advmod	fluorescently,labeled
R1582	T4197	T4198	amod	labeled,dextran
R1583	T4198	T4195	dobj	dextran,applying
R1584	T4199	T4195	prep	to,applying
R1585	T4287	T4284	ccomp	are,assess
R1586	T4200	T4201	amod	cut,roots
R1587	T4201	T4199	pobj	roots,to
R1588	T4202	T4201	amod	dorsal,roots
R1589	T4288	T4287	nsubj	synapses,are
R159	T1514	T1512	attr	process,is
R1590	T4203	T4204	punct	(,2D
R1591	T4204	T4195	parataxis	2D,applying
R1592	T4205	T4204	compound	Figure,2D
R1593	T4289	T4288	prep	between,synapses
R1594	T4206	T4207	punct	–,2F
R1595	T4207	T4204	prep	2F,2D
R1596	T4208	T4204	punct	),2D
R1597	T4290	T4291	compound	Ia,afferents
R1598	T4209	T4170	punct	.,used
R1599	T4291	T4289	pobj	afferents,between
R160	T1515	T1514	amod	protracted,process
R1600	T4211	T4212	advcl	Using,found
R1601	T4213	T4214	det	both,assays
R1602	T4292	T4291	cc	and,afferents
R1603	T4214	T4211	dobj	assays,Using
R1604	T4293	T4294	compound	motor,neurons
R1605	T4215	T4212	punct	", ",found
R1606	T4294	T4291	conj	neurons,afferents
R1607	T4216	T4212	nsubj	we,found
R1608	T4217	T4218	amod	extensive,rescue
R1609	T4218	T4212	dobj	rescue,found
R161	T1516	T1517	prep	during,express
R1610	T4295	T4287	acomp	functional,are
R1611	T4219	T4218	prep	of,rescue
R1612	T4220	T4221	det	the,projections
R1613	T4296	T4287	prep	in,are
R1614	T4221	T4219	pobj	projections,of
R1615	T4222	T4221	prep	into,projections
R1616	T4223	T4224	det	the,horn
R1617	T4224	T4222	pobj	horn,into
R1618	T4225	T4224	amod	ventral,horn
R1619	T4297	T4298	nmod	Er81EWS,Pea3
R162	T1517	T1514	relcl	express,process
R1620	T4226	T4224	prep	of,horn
R1621	T4227	T4228	det	the,cord
R1622	T4228	T4226	pobj	cord,of
R1623	T4298	T4300	nmod	Pea3,mice
R1624	T4229	T4228	amod	spinal,cord
R1625	T4230	T4212	prep	in,found
R1626	T4299	T4298	punct	-,Pea3
R1627	T4231	T4232	nmod	Er81EWS,Pea3
R1628	T4232	T4234	nmod	Pea3,mice
R1629	T4233	T4232	punct	-,Pea3
R163	T1518	T1516	pobj	which,during
R1630	T4300	T4296	pobj	mice,in
R1631	T4234	T4230	pobj	mice,in
R1632	T4235	T4232	punct	/,Pea3
R1633	T4236	T4232	punct	−,Pea3
R1634	T4301	T4298	punct	/,Pea3
R1635	T4237	T4238	punct	(,2C
R1636	T4238	T4212	parataxis	2C,found
R1637	T4239	T4238	compound	Figure,2C
R1638	T4302	T4298	punct	−,Pea3
R1639	T4240	T4238	cc	and,2C
R164	T1519	T1520	advmod	newly,generated
R1640	T4241	T4238	conj	2F,2C
R1641	T4242	T4238	punct	),2C
R1642	T4303	T4285	punct	", ",performed
R1643	T4243	T4212	punct	.,found
R1644	T4304	T4285	nsubj	we,performed
R1645	T4245	T4246	prep	Within,formed
R1646	T4247	T4248	det	the,horn
R1647	T4305	T4306	amod	intracellular,recordings
R1648	T4248	T4245	pobj	horn,Within
R1649	T4249	T4248	amod	ventral,horn
R165	T1520	T1521	amod	generated,neurons
R1650	T4250	T4246	punct	", ",formed
R1651	T4306	T4285	dobj	recordings,performed
R1652	T4251	T4252	compound	Ia,afferents
R1653	T4252	T4246	nsubj	afferents,formed
R1654	T4253	T4252	prep	in,afferents
R1655	T4307	T4306	prep	from,recordings
R1656	T4254	T4255	preconj	both,type
R1657	T4255	T4258	nmod	type,mice
R1658	T4256	T4255	amod	wild,type
R1659	T4257	T4255	punct	-,type
R166	T1521	T1517	nsubj	neurons,express
R1660	T4308	T4309	amod	identified,neurons
R1661	T4258	T4253	pobj	mice,in
R1662	T4259	T4255	cc	and,type
R1663	T4260	T4261	compound	Er81EWS,Pea3
R1664	T4309	T4307	pobj	neurons,from
R1665	T4261	T4255	conj	Pea3,type
R1666	T4262	T4261	punct	-,Pea3
R1667	T4263	T4261	punct	/,Pea3
R1668	T4264	T4261	punct	−,Pea3
R1669	T4310	T4309	compound	quadriceps,neurons
R167	T1522	T1523	amod	distinct,programs
R1670	T4311	T4309	compound	motor,neurons
R1671	T4312	T4285	prep	after,performed
R1672	T4313	T4312	pobj	stimulation,after
R1673	T4314	T4313	prep	of,stimulation
R1674	T4371	T4368	punct	", ",8
R1675	T4315	T4314	pobj	nerves,of
R1676	T4372	T4373	quantmod	10.9,1
R1677	T4373	T4375	nummod	1,mV
R1678	T4316	T4315	acl	innervating,nerves
R1679	T4374	T4373	punct	±,1
R168	T1523	T1517	dative	programs,express
R1680	T4375	T4368	dep	mV,8
R1681	T4376	T4368	punct	", ",8
R1682	T4317	T4318	det	the,group
R1683	T4377	T4368	nsubj	n,8
R1684	T4378	T4368	punct	=,8
R1685	T4379	T4349	punct	),S2
R1686	T4318	T4316	dobj	group,innervating
R1687	T4380	T4324	punct	.,found
R1688	T4382	T4383	advmod	Together,suggest
R1689	T4319	T4318	compound	quadriceps,group
R169	T1524	T1523	amod	cellular,programs
R1690	T4384	T4383	punct	", ",suggest
R1691	T4320	T4318	compound	muscle,group
R1692	T4321	T4285	punct	.,performed
R1693	T4385	T4386	det	these,findings
R1694	T4386	T4383	nsubj	findings,suggest
R1695	T4323	T4324	nsubj	We,found
R1696	T4387	T4388	mark	that,direct
R1697	T4388	T4383	ccomp	direct,suggest
R1698	T4389	T4388	prep	in,direct
R1699	T4325	T4326	det	no,difference
R170	T1525	T1524	cc	and,cellular
R1700	T4390	T4391	det	the,absence
R1701	T4391	T4389	pobj	absence,in
R1702	T4392	T4391	prep	of,absence
R1703	T4393	T4392	pobj	Er81,of
R1704	T4326	T4324	dobj	difference,found
R1705	T4327	T4326	amod	significant,difference
R1706	T4328	T4326	prep	in,difference
R1707	T4329	T4330	det	the,amplitude
R1708	T4394	T4388	punct	", ",direct
R1709	T4330	T4328	pobj	amplitude,in
R171	T1526	T1524	conj	molecular,cellular
R1710	T4395	T4396	compound	EWS,Pea3
R1711	T4396	T4388	nsubj	Pea3,direct
R1712	T4331	T4330	compound	input,amplitude
R1713	T4397	T4396	punct	-,Pea3
R1714	T4398	T4388	aux	can,direct
R1715	T4332	T4330	prep	to,amplitude
R1716	T4399	T4400	det	the,process
R1717	T4400	T4388	dobj	process,direct
R1718	T4401	T4400	amod	complex,process
R1719	T4333	T4334	compound	quadriceps,neurons
R172	T1527	T1517	prep	at,express
R1720	T4402	T4400	amod	biological,process
R1721	T4403	T4400	prep	of,process
R1722	T4404	T4405	amod	correct,termination
R1723	T4405	T4403	pobj	termination,of
R1724	T4406	T4405	amod	laminar,termination
R1725	T4407	T4405	prep	within,termination
R1726	T4334	T4332	pobj	neurons,to
R1727	T4408	T4409	det	the,cord
R1728	T4409	T4407	pobj	cord,within
R1729	T4410	T4409	amod	ventral,cord
R173	T1528	T1529	amod	precise,times
R1730	T4335	T4334	compound	motor,neurons
R1731	T4411	T4409	amod	spinal,cord
R1732	T4412	T4400	cc	and,process
R1733	T4413	T4414	det	the,formation
R1734	T4336	T4337	advmod	when,comparing
R1735	T4414	T4400	conj	formation,process
R1736	T4415	T4414	prep	of,formation
R1737	T4416	T4415	pobj	synapses,of
R1738	T4337	T4324	advcl	comparing,found
R1739	T4417	T4416	prep	with,synapses
R174	T1529	T1527	pobj	times,at
R1740	T4418	T4419	compound	motor,neurons
R1741	T4338	T4339	amod	wild,type
R1742	T4419	T4417	pobj	neurons,with
R1743	T4420	T4421	punct	(,2J
R1744	T4339	T4337	dobj	type,comparing
R1745	T4421	T4388	parataxis	2J,direct
R1746	T4422	T4421	compound	Figure,2J
R1747	T4423	T4424	punct	–,2L
R1748	T4340	T4339	punct	-,type
R1749	T4424	T4421	prep	2L,2J
R175	T1530	T1517	prep	during,express
R1750	T4425	T4421	punct	),2J
R1751	T4426	T4388	punct	", ",direct
R1752	T4341	T4337	prep	to,comparing
R1753	T4427	T4428	advmod	thus,identifying
R1754	T4428	T4388	advcl	identifying,direct
R1755	T4429	T4430	det	an,factor
R1756	T4342	T4343	compound	Er81EWS,Pea3
R1757	T4430	T4428	dobj	factor,identifying
R1758	T4431	T4430	compound	ETS,factor
R1759	T4343	T4341	pobj	Pea3,to
R176	T1531	T1532	poss	their,maturation
R1760	T4432	T4430	compound	transcription,factor
R1761	T4433	T4430	amod	suitable,factor
R1762	T4434	T4433	prep	for,suitable
R1763	T4344	T4343	punct	-,Pea3
R1764	T4435	T4436	amod	heterochronic,experiments
R1765	T4436	T4434	pobj	experiments,for
R1766	T4437	T4436	compound	expression,experiments
R1767	T4345	T4343	punct	/,Pea3
R1768	T4438	T4436	prep	in,experiments
R1769	T4439	T4440	compound	DRG,neurons
R177	T1532	T1530	pobj	maturation,during
R1770	T4440	T4438	pobj	neurons,in
R1771	T4346	T4343	punct	−,Pea3
R1772	T4441	T4383	punct	.,suggest
R1773	T4347	T4341	pobj	mice,to
R1774	T4348	T4349	punct	(,S2
R1775	T4349	T4337	parataxis	S2,comparing
R1776	T4350	T4349	compound	Figure,S2
R1777	T4351	T4349	punct	;,S2
R1778	T4352	T4353	amod	wild,type
R1779	T4353	T4355	dep	type,11
R178	T1533	T1517	punct	: ,express
R1780	T4354	T4353	punct	-,type
R1781	T4355	T4349	dep	11,S2
R1782	T4356	T4355	punct	", ",11
R1783	T4357	T4358	quantmod	10.6,0.9
R1784	T4358	T4360	nummod	0.9,mV
R1785	T4359	T4358	punct	±,0.9
R1786	T4360	T4355	dep	mV,11
R1787	T4361	T4355	punct	", ",11
R1788	T4362	T4355	nsubj	n,11
R1789	T4363	T4355	punct	=,11
R179	T1534	T1535	amod	long,distance
R1790	T4364	T4349	punct	;,S2
R1791	T4365	T4366	compound	Er81EWS,Pea3
R1792	T4366	T4368	dep	Pea3,8
R1793	T4367	T4366	punct	-,Pea3
R1794	T4368	T4349	dep	8,S2
R1795	T4369	T4366	punct	/,Pea3
R1796	T4370	T4366	punct	−,Pea3
R1797	T5254	T5255	amod	Precocious,Expression
R1798	T5255	T5256	nsubj	Expression,Leads
R1799	T5257	T5255	prep	of,Expression
R180	T1535	T1537	compound	distance,outgrowth
R1800	T5258	T5259	compound	EWS,Pea3
R1801	T5259	T5257	pobj	Pea3,of
R1802	T5260	T5259	punct	-,Pea3
R1803	T5261	T5255	prep	in,Expression
R1804	T5262	T5263	compound	DRG,Neurons
R1805	T5263	T5261	pobj	Neurons,in
R1806	T5264	T5256	prep	to,Leads
R1807	T5265	T5266	amod	Axonal,Defects
R1808	T5266	T5264	pobj	Defects,to
R1809	T5267	T5266	compound	Projection,Defects
R181	T1536	T1535	punct	-,distance
R1810	T5269	T5270	aux	To,address
R1811	T5270	T5271	advcl	address,expressed
R1812	T5272	T5273	det	the,consequences
R1813	T5273	T5270	dobj	consequences,address
R1814	T5274	T5273	prep	of,consequences
R1815	T5275	T5276	amod	precocious,signaling
R1816	T5276	T5274	pobj	signaling,of
R1817	T5277	T5276	compound	ETS,signaling
R1818	T5278	T5273	prep	for,consequences
R1819	T5279	T5280	amod	proprioceptive,afferent
R182	T1537	T1517	dobj	outgrowth,express
R1820	T5280	T5281	nmod	afferent,differentiation
R1821	T5281	T5278	pobj	differentiation,for
R1822	T5282	T5271	punct	", ",expressed
R1823	T5283	T5271	nsubj	we,expressed
R1824	T5284	T5271	advmod	next,expressed
R1825	T5285	T5286	compound	EWS,Pea3
R1826	T5286	T5271	dobj	Pea3,expressed
R1827	T5287	T5286	punct	-,Pea3
R1828	T5288	T5271	prep	in,expressed
R1829	T5289	T5290	compound	DRG,neurons
R183	T1538	T1537	compound	axon,outgrowth
R1830	T5290	T5288	pobj	neurons,in
R1831	T5291	T5292	advmod	as,soon
R1832	T5292	T5271	advmod	soon,expressed
R1833	T5293	T5294	mark	as,became
R1834	T5294	T5292	advcl	became,soon
R1835	T5295	T5294	nsubj	they,became
R1836	T5296	T5294	acomp	post-mitotic,became
R1837	T5297	T5271	punct	.,expressed
R1838	T5299	T5300	nsubj	We,used
R1839	T5301	T5302	det	a,system
R184	T1539	T1537	punct	", ",outgrowth
R1840	T5302	T5300	dobj	system,used
R1841	T5303	T5302	amod	binary,system
R1842	T5304	T5302	nmod	mouse,system
R1843	T5305	T5302	amod	genetic,system
R1844	T5306	T5302	prep	based,system
R1845	T5307	T5306	prep	on,based
R1846	T5308	T5309	compound	Cre,recombinase
R1847	T5309	T5311	npadvmod	recombinase,mediated
R1848	T5310	T5309	punct	-,recombinase
R1849	T5311	T5313	amod	mediated,excision
R185	T1540	T1541	amod	subsequent,branching
R1850	T5312	T5311	punct	-,mediated
R1851	T5313	T5307	pobj	excision,on
R1852	T5314	T5313	prep	of,excision
R1853	T5315	T5316	det	a,cassette
R1854	T5316	T5314	pobj	cassette,of
R1855	T5317	T5316	amod	transcriptional,cassette
R1856	T5318	T5316	compound	stop,cassette
R1857	T5319	T5316	acl	flanked,cassette
R1858	T5320	T5319	agent	by,flanked
R1859	T5321	T5322	compound	loxP,sites
R186	T1541	T1537	conj	branching,outgrowth
R1860	T5322	T5320	pobj	sites,by
R1861	T5323	T5300	punct	.,used
R1862	T5325	T5326	compound	Targeting,cassettes
R1863	T5326	T5327	nsubjpass	cassettes,integrated
R1864	T5328	T5327	auxpass	were,integrated
R1865	T5329	T5327	prep	into,integrated
R1866	T5330	T5331	det	the,locus
R1867	T5331	T5329	pobj	locus,into
R1868	T5332	T5331	compound	Tau,locus
R1869	T5333	T5334	aux	to,generate
R187	T1542	T1541	amod	terminal,branching
R1870	T5334	T5327	advcl	generate,integrated
R1871	T5335	T5336	nummod	two,strains
R1872	T5336	T5334	dobj	strains,generate
R1873	T5337	T5336	prep	of,strains
R1874	T5338	T5337	pobj	mice,of
R1875	T5339	T5340	advmod	conditionally,expressing
R1876	T5340	T5336	acl	expressing,strains
R1877	T5341	T5342	preconj	either,Pea3
R1878	T5342	T5340	dobj	Pea3,expressing
R1879	T5343	T5342	compound	EWS,Pea3
R188	T1543	T1541	punct	", ",branching
R1880	T5344	T5342	punct	-,Pea3
R1881	T5345	T5342	cc	or,Pea3
R1882	T5346	T5347	det	a,protein
R1883	T5347	T5342	conj	protein,Pea3
R1884	T5348	T5349	npadvmod	membrane,targeted
R1885	T5349	T5347	amod	targeted,protein
R1886	T5350	T5349	punct	-,targeted
R1887	T5351	T5347	amod	green,protein
R1888	T5352	T5347	amod	fluorescent,protein
R1889	T5353	T5347	punct	(,protein
R189	T1544	T1541	cc	and,branching
R1890	T5354	T5347	appos	mGFP,protein
R1891	T5355	T5347	punct	),protein
R1892	T5356	T5357	aux	to,trace
R1893	T5357	T5340	advcl	trace,expressing
R1894	T5358	T5359	amod	axonal,projections
R1895	T5359	T5357	dobj	projections,trace
R1896	T5360	T5359	prep	of,projections
R1897	T5361	T5362	compound	DRG,neurons
R1898	T5362	T5360	pobj	neurons,of
R1899	T5363	T5364	punct	(,25
R190	T1545	T1546	advmod	finally,synaptogenesis
R1900	T5364	T5334	parataxis	25,generate
R1901	T5365	T5366	compound	Figure,S3
R1902	T5366	T5364	dep	S3,25
R1903	T5367	T5364	punct	;,25
R1904	T5368	T5364	punct	[,25
R1905	T5369	T5364	punct	],25
R1906	T5370	T5364	punct	),25
R1907	T5371	T5327	punct	.,integrated
R1908	T5373	T5374	nsubj	Embryos,showed
R1909	T5375	T5373	amod	positive,Embryos
R191	T1546	T1541	conj	synaptogenesis,branching
R1910	T5376	T5375	prep	for,positive
R1911	T5377	T5378	preconj	either,Isl1Cre
R1912	T5378	T5379	nmod	Isl1Cre,alleles
R1913	T5379	T5376	pobj	alleles,for
R1914	T5380	T5378	punct	/,Isl1Cre
R1915	T5381	T5378	punct	+,Isl1Cre
R1916	T5382	T5378	cc	and,Isl1Cre
R1917	T5383	T5384	compound	TauEWS,Pea3
R1918	T5384	T5378	conj	Pea3,Isl1Cre
R1919	T5385	T5384	punct	-,Pea3
R192	T1547	T1512	punct	.,is
R1920	T5386	T5384	punct	/,Pea3
R1921	T5387	T5384	punct	+,Pea3
R1922	T5388	T5378	cc	or,Isl1Cre
R1923	T5389	T5378	conj	Isl1Cre,Isl1Cre
R1924	T5390	T5389	punct	/,Isl1Cre
R1925	T5391	T5389	punct	+,Isl1Cre
R1926	T5392	T5389	cc	and,Isl1Cre
R1927	T5393	T5389	conj	TaumGFP,Isl1Cre
R1928	T5394	T5393	punct	/,TaumGFP
R1929	T5395	T5393	punct	+,TaumGFP
R193	T1549	T1550	amod	Many,aspects
R1930	T5396	T5397	amod	efficient,activation
R1931	T5397	T5374	dobj	activation,showed
R1932	T5398	T5397	prep	of,activation
R1933	T5399	T5400	det	the,allele
R1934	T5400	T5398	pobj	allele,of
R1935	T5401	T5400	amod	silent,allele
R1936	T5402	T5400	compound	Tau,allele
R1937	T5403	T5374	prep	in,showed
R1938	T5404	T5405	nummod	95,%
R1939	T5405	T5403	pobj	%,in
R194	T1550	T1552	nsubj	aspects,appear
R1940	T5406	T5407	cc	or,more
R1941	T5407	T5405	nummod	more,%
R1942	T5408	T5405	prep	of,%
R1943	T5409	T5410	det	all,neurons
R1944	T5410	T5408	pobj	neurons,of
R1945	T5411	T5410	compound	DRG,neurons
R1946	T5412	T5405	punct	", ",%
R1947	T5413	T5405	prep	including,%
R1948	T5414	T5415	amod	proprioceptive,afferents
R1949	T5415	T5413	pobj	afferents,including
R195	T1551	T1550	amod	important,aspects
R1950	T5416	T5374	punct	", ",showed
R1951	T5417	T5374	prep	at,showed
R1952	T5418	T5419	det	all,levels
R1953	T5419	T5417	pobj	levels,at
R1954	T5420	T5419	amod	segmental,levels
R1955	T5421	T5422	punct	(,S3
R1956	T5422	T5374	parataxis	S3,showed
R1957	T5423	T5422	compound	Figure,S3
R1958	T5424	T5422	punct	),S3
R1959	T5425	T5374	punct	.,showed
R196	T1553	T1550	prep	of,aspects
R1960	T5427	T5428	nsubj	We,assessed
R1961	T5429	T5428	advmod	first,assessed
R1962	T5430	T5431	det	the,influence
R1963	T5431	T5428	dobj	influence,assessed
R1964	T5432	T5431	prep	of,influence
R1965	T5433	T5434	compound	EWS,Pea3
R1966	T5434	T5436	compound	Pea3,expression
R1967	T5435	T5434	punct	-,Pea3
R1968	T5436	T5432	pobj	expression,of
R1969	T5437	T5436	prep	in,expression
R197	T1554	T1555	amod	neuronal,character
R1970	T5438	T5439	amod	early,neurons
R1971	T5439	T5437	pobj	neurons,in
R1972	T5440	T5439	amod	post-mitotic,neurons
R1973	T5441	T5439	compound	DRG,neurons
R1974	T5442	T5431	prep	on,influence
R1975	T5443	T5444	det	the,establishment
R1976	T5444	T5442	pobj	establishment,on
R1977	T5445	T5444	prep	of,establishment
R1978	T5446	T5447	amod	afferent,projections
R1979	T5447	T5445	pobj	projections,of
R198	T1555	T1553	pobj	character,of
R1980	T5448	T5447	prep	into,projections
R1981	T5449	T5450	det	the,cord
R1982	T5450	T5448	pobj	cord,into
R1983	T5451	T5450	amod	spinal,cord
R1984	T5452	T5444	acl	using,establishment
R1985	T5453	T5454	det	the,allele
R1986	T5454	T5452	dobj	allele,using
R1987	T5455	T5454	nmod	TaumGFP,allele
R1988	T5456	T5454	punct	/,allele
R1989	T5457	T5454	punct	+,allele
R199	T1556	T1557	aux	to,acquired
R1990	T5458	T5454	cc	or,allele
R1991	T5459	T5460	det	a,protein
R1992	T5460	T5454	conj	protein,allele
R1993	T5461	T5462	compound	Thy1,promoter
R1994	T5462	T5464	npadvmod	promoter,driven
R1995	T5463	T5462	punct	-,promoter
R1996	T5464	T5460	amod	driven,protein
R1997	T5465	T5464	punct	-,driven
R1998	T5466	T5460	nmod	synaptophysin,protein
R1999	T5467	T5460	amod	green,protein
R200	T1557	T1552	xcomp	acquired,appear
R2000	T5468	T5460	amod	fluorescent,protein
R2001	T5469	T5460	punct	(,protein
R2002	T5470	T5460	appos	spGFP,protein
R2003	T5471	T5428	punct	),assessed
R2004	T5472	T5473	mark	with,restricted
R2005	T5473	T5428	advcl	restricted,assessed
R2006	T5474	T5475	det	an,profile
R2007	T5475	T5473	nsubj	profile,restricted
R2008	T5476	T5475	compound	expression,profile
R2009	T5477	T5473	prep	to,restricted
R201	T1558	T1557	auxpass	be,acquired
R2010	T5478	T5479	nmod	DRG,neurons
R2011	T5479	T5477	pobj	neurons,to
R2012	T5480	T5479	amod	sensory,neurons
R2013	T5481	T5473	prep	at,restricted
R2014	T5482	T5483	amod	embryonic,day
R2015	T5483	T5481	pobj	day,at
R2016	T5484	T5483	punct	(,day
R2017	T5485	T5483	appos	E,day
R2018	T5486	T5483	punct	),day
R2019	T5487	T5483	nummod	13.5,day
R202	T1559	T1557	prep	through,acquired
R2020	T5488	T5489	punct	(,Figure
R2021	T5489	T5473	parataxis	Figure,restricted
R2022	T5490	T5489	dep	Thy1spGFP,Figure
R2023	T5491	T5489	punct	;,Figure
R2024	T5492	T5489	punct	[,Figure
R2025	T5493	T5489	dep	25,Figure
R2026	T5494	T5489	punct	],Figure
R2027	T5495	T5489	punct	),Figure
R2028	T5496	T5489	punct	(,Figure
R2029	T5497	T5489	nummod	3,Figure
R203	T1560	T1561	det	the,expression
R2030	T5498	T5489	punct	),Figure
R2031	T5499	T5428	punct	.,assessed
R2032	T5501	T5502	prep	In,failed
R2033	T5503	T5501	pobj	contrast,In
R2034	T5504	T5503	prep	to,contrast
R2035	T5505	T5506	amod	wild,type
R2036	T5506	T5508	nmod	type,projections
R2037	T5507	T5506	punct	-,type
R2038	T5508	T5504	pobj	projections,to
R2039	T5509	T5508	amod	proprioceptive,projections
R204	T1561	T1559	pobj	expression,through
R2040	T5510	T5508	amod	afferent,projections
R2041	T5511	T5512	punct	(,3A
R2042	T5512	T5508	parataxis	3A,projections
R2043	T5513	T5512	compound	Figure,3A
R2044	T5514	T5515	punct	–,3C
R2045	T5515	T5512	prep	3C,3A
R2046	T5516	T5512	punct	),3A
R2047	T5517	T5502	punct	", ",failed
R2048	T5518	T5519	nmod	GFP,afferents
R2049	T5519	T5502	nsubj	afferents,failed
R205	T1562	T1561	prep	of,expression
R2050	T5520	T5518	punct	+,GFP
R2051	T5521	T5519	amod	sensory,afferents
R2052	T5522	T5519	prep	in,afferents
R2053	T5523	T5524	nmod	TauEWS,Pea3
R2054	T5524	T5526	nmod	Pea3,embryos
R2055	T5525	T5524	punct	-,Pea3
R2056	T5526	T5522	pobj	embryos,in
R2057	T5527	T5524	punct	/,Pea3
R2058	T5528	T5524	punct	+,Pea3
R2059	T5529	T5524	appos	Isl1Cre,Pea3
R206	T1563	T1564	compound	transcription,factors
R2060	T5530	T5529	punct	/,Isl1Cre
R2061	T5531	T5529	punct	+,Isl1Cre
R2062	T5532	T5533	aux	to,invade
R2063	T5533	T5502	xcomp	invade,failed
R2064	T5534	T5535	det	the,cord
R2065	T5535	T5533	dobj	cord,invade
R2066	T5536	T5535	amod	spinal,cord
R2067	T5537	T5502	cc	and,failed
R2068	T5538	T5539	advmod	instead,found
R2069	T5539	T5502	conj	found,failed
R207	T1564	T1562	pobj	factors,of
R2070	T5540	T5539	auxpass	were,found
R2071	T5541	T5539	oprd	in,found
R2072	T5542	T5543	det	an,position
R2073	T5543	T5541	pobj	position,in
R2074	T5544	T5543	advmod	extreme,position
R2075	T5545	T5543	amod	lateral,position
R2076	T5546	T5543	prep	at,position
R2077	T5547	T5548	det	the,zone
R2078	T5548	T5546	pobj	zone,at
R2079	T5549	T5550	amod	dorsal,root
R208	T1565	T1561	prep	at,expression
R2080	T5550	T5548	compound	root,zone
R2081	T5551	T5548	compound	entry,zone
R2082	T5552	T5539	punct	", ",found
R2083	T5553	T5554	det	a,phenotype
R2084	T5554	T5539	npadvmod	phenotype,found
R2085	T5555	T5554	acl	observed,phenotype
R2086	T5556	T5557	advmod	at,least
R2087	T5557	T5558	advmod	least,up
R2088	T5558	T5555	prep	up,observed
R2089	T5559	T5558	prep	to,up
R209	T1566	T1567	compound	progenitor,stages
R2090	T5560	T5559	pobj	E18.5,to
R2091	T5561	T5562	punct	(,3A
R2092	T5562	T5539	parataxis	3A,found
R2093	T5563	T5562	compound	Figure,3A
R2094	T5564	T5565	punct	–,3C
R2095	T5565	T5562	prep	3C,3A
R2096	T5566	T5562	cc	and,3A
R2097	T5567	T5562	conj	3G,3A
R2098	T5568	T5569	punct	–,3I
R2099	T5569	T5567	prep	3I,3G
R210	T1567	T1565	pobj	stages,at
R2100	T5570	T5562	punct	;,3A
R2101	T5571	T5572	nsubj	data,shown
R2102	T5572	T5562	ccomp	shown,3A
R2103	T5573	T5572	neg	not,shown
R2104	T5574	T5562	punct	),3A
R2105	T5575	T5502	punct	.,failed
R2106	T5577	T5578	nsubj	We,visualized
R2107	T5579	T5578	advmod	next,visualized
R2108	T5580	T5581	det	the,path
R2109	T5581	T5578	dobj	path,visualized
R211	T1568	T1567	compound	cell,stages
R2110	T5582	T5581	prep	of,path
R2111	T5583	T5584	amod	sensory,afferent
R2112	T5584	T5585	nmod	afferent,projections
R2113	T5585	T5582	pobj	projections,of
R2114	T5586	T5585	prep	towards,projections
R2115	T5587	T5588	det	the,zone
R2116	T5588	T5586	pobj	zone,towards
R2117	T5589	T5590	amod	dorsal,root
R2118	T5590	T5588	compound	root,zone
R2119	T5591	T5588	compound	entry,zone
R212	T1569	T1552	punct	", ",appear
R2120	T5592	T5578	prep	in,visualized
R2121	T5593	T5594	nmod	TauEWS,Isl1Cre
R2122	T5594	T5599	nmod	Isl1Cre,embryos
R2123	T5595	T5594	punct	-,Isl1Cre
R2124	T5596	T5594	nmod	Pea3,Isl1Cre
R2125	T5597	T5594	punct	/,Isl1Cre
R2126	T5598	T5594	punct	+,Isl1Cre
R2127	T5599	T5592	pobj	embryos,in
R2128	T5600	T5594	punct	/,Isl1Cre
R2129	T5601	T5594	punct	+,Isl1Cre
R213	T1570	T1571	mark	whereas,depend
R2130	T5602	T5578	prep	by,visualized
R2131	T5603	T5602	pcomp	injecting,by
R2132	T5604	T5605	advmod	fluorescently,labeled
R2133	T5605	T5606	amod	labeled,dextran
R2134	T5606	T5603	dobj	dextran,injecting
R2135	T5607	T5603	prep	into,injecting
R2136	T5608	T5609	det	an,DRG
R2137	T5609	T5607	pobj	DRG,into
R2138	T5610	T5609	amod	individual,DRG
R2139	T5611	T5612	punct	(,3M
R214	T1571	T1552	advcl	depend,appear
R2140	T5612	T5578	parataxis	3M,visualized
R2141	T5613	T5612	dep	L3,3M
R2142	T5614	T5612	punct	;,3M
R2143	T5615	T5616	nsubj	n,3
R2144	T5616	T5612	ccomp	3,3M
R2145	T5617	T5616	punct	=,3
R2146	T5618	T5612	punct	;,3M
R2147	T5619	T5612	compound	Figure,3M
R2148	T5620	T5621	punct	–,3Q
R2149	T5621	T5612	prep	3Q,3M
R215	T1572	T1571	nsubj	others,depend
R2150	T5622	T5612	punct	),3M
R2151	T5623	T5578	punct	.,visualized
R2152	T5625	T5626	amod	Sensory,afferents
R2153	T5626	T5627	nsubj	afferents,bifurcated
R2154	T5628	T5626	prep	in,afferents
R2155	T5629	T5630	nmod	E13.5,embryos
R2156	T5630	T5628	pobj	embryos,in
R2157	T5631	T5632	amod	wild,type
R2158	T5632	T5630	compound	type,embryos
R2159	T5633	T5632	punct	-,type
R216	T1573	T1571	prep	on,depend
R2160	T5634	T5627	prep	at,bifurcated
R2161	T5635	T5636	poss	their,point
R2162	T5636	T5634	pobj	point,at
R2163	T5637	T5636	amod	lateral,point
R2164	T5638	T5636	amod	spinal,point
R2165	T5639	T5636	compound	entry,point
R2166	T5640	T5627	punct	", ",bifurcated
R2167	T5641	T5627	cc	and,bifurcated
R2168	T5642	T5627	conj	projected,bifurcated
R2169	T5643	T5642	advmod	rostrally,projected
R217	T1574	T1573	pobj	expression,on
R2170	T5644	T5643	cc	and,rostrally
R2171	T5645	T5643	conj	caudally,rostrally
R2172	T5646	T5642	prep	over,projected
R2173	T5647	T5648	nummod	six,levels
R2174	T5648	T5646	pobj	levels,over
R2175	T5649	T5647	cc	or,six
R2176	T5650	T5647	conj	more,six
R2177	T5651	T5648	amod	segmental,levels
R2178	T5652	T5653	mark	while,approaching
R2179	T5653	T5627	advcl	approaching,bifurcated
R218	T1575	T1576	advmod	immediately,upon
R2180	T5654	T5653	advmod	gradually,approaching
R2181	T5655	T5656	det	the,midline
R2182	T5656	T5653	dobj	midline,approaching
R2183	T5657	T5658	punct	(,3M
R2184	T5658	T5653	parataxis	3M,approaching
R2185	T5659	T5658	compound	Figure,3M
R2186	T5660	T5658	punct	),3M
R2187	T5661	T5627	punct	.,bifurcated
R2188	T5663	T5664	amod	Sensory,afferents
R2189	T5664	T5665	nsubj	afferents,bifurcated
R219	T1576	T1571	prep	upon,depend
R2190	T5666	T5664	prep	in,afferents
R2191	T5667	T5668	nmod	TauEWS,Isl1Cre
R2192	T5668	T5673	nmod	Isl1Cre,embryos
R2193	T5669	T5668	punct	-,Isl1Cre
R2194	T5670	T5668	nmod	Pea3,Isl1Cre
R2195	T5671	T5668	punct	/,Isl1Cre
R2196	T5672	T5668	punct	+,Isl1Cre
R2197	T5673	T5666	pobj	embryos,in
R2198	T5674	T5668	punct	/,Isl1Cre
R2199	T5675	T5668	punct	+,Isl1Cre
R220	T1577	T1578	compound	cell,cycle
R2200	T5676	T5665	advmod	also,bifurcated
R2201	T5677	T5665	prep	at,bifurcated
R2202	T5678	T5679	det	the,point
R2203	T5679	T5677	pobj	point,at
R2204	T5680	T5679	compound	entry,point
R2205	T5681	T5665	punct	", ",bifurcated
R2206	T5682	T5683	mark	although,continued
R2207	T5683	T5665	advcl	continued,bifurcated
R2208	T5684	T5685	advmod	approximately,5
R2209	T5685	T5686	nummod	5,%
R221	T1578	T1579	compound	cycle,exit
R2210	T5686	T5683	nsubj	%,continued
R2211	T5687	T5686	prep	of,%
R2212	T5688	T5689	amod	afferent,fibers
R2213	T5689	T5687	pobj	fibers,of
R2214	T5690	T5691	aux	to,grow
R2215	T5691	T5683	xcomp	grow,continued
R2216	T5692	T5691	prep	towards,grow
R2217	T5693	T5694	det	the,midline
R2218	T5694	T5692	pobj	midline,towards
R2219	T5695	T5696	punct	(,3O
R222	T1579	T1576	pobj	exit,upon
R2220	T5696	T5665	parataxis	3O,bifurcated
R2221	T5697	T5696	compound	Figure,3O
R2222	T5698	T5696	cc	and,3O
R2223	T5699	T5696	conj	3Q,3O
R2224	T5700	T5696	punct	),3O
R2225	T5701	T5665	punct	.,bifurcated
R2226	T5703	T5704	mark	While,were
R2227	T5704	T5709	advcl	were,failed
R2228	T5705	T5706	amod	rostro,caudal
R2229	T5706	T5708	amod	caudal,projections
R223	T1580	T1581	punct	[,1
R2230	T5707	T5706	punct	-,caudal
R2231	T5708	T5704	nsubj	projections,were
R2232	T5710	T5704	acomp	present,were
R2233	T5711	T5704	prep	in,were
R2234	T5712	T5713	nmod	TauEWS,Isl1Cre
R2235	T5713	T5718	nmod	Isl1Cre,embryos
R2236	T5714	T5713	punct	-,Isl1Cre
R2237	T5715	T5713	nmod	Pea3,Isl1Cre
R2238	T5716	T5713	punct	/,Isl1Cre
R2239	T5717	T5713	punct	+,Isl1Cre
R224	T1581	T1571	parataxis	1,depend
R2240	T5718	T5711	pobj	embryos,in
R2241	T5719	T5713	punct	/,Isl1Cre
R2242	T5720	T5713	punct	+,Isl1Cre
R2243	T5721	T5709	punct	", ",failed
R2244	T5722	T5723	amod	afferent,fibers
R2245	T5723	T5709	nsubj	fibers,failed
R2246	T5724	T5725	aux	to,approach
R2247	T5725	T5709	xcomp	approach,failed
R2248	T5726	T5727	det	the,midline
R2249	T5727	T5725	dobj	midline,approach
R225	T1582	T1581	punct	],1
R2250	T5728	T5725	prep	at,approach
R2251	T5729	T5730	amod	distal,segments
R2252	T5730	T5728	pobj	segments,at
R2253	T5731	T5709	cc	and,failed
R2254	T5732	T5709	conj	continued,failed
R2255	T5733	T5734	aux	to,occupy
R2256	T5734	T5732	xcomp	occupy,continued
R2257	T5735	T5736	det	an,position
R2258	T5736	T5734	dobj	position,occupy
R2259	T5737	T5736	advmod	extreme,position
R226	T1583	T1552	punct	.,appear
R2260	T5738	T5736	amod	lateral,position
R2261	T5739	T5740	punct	(,3O
R2262	T5740	T5736	parataxis	3O,position
R2263	T5741	T5740	compound	Figure,3O
R2264	T5742	T5740	punct	),3O
R2265	T5743	T5734	punct	", ",occupy
R2266	T5744	T5734	advcl	consistent,occupy
R2267	T5745	T5744	prep	with,consistent
R2268	T5746	T5747	det	the,analysis
R2269	T5747	T5745	pobj	analysis,with
R227	T1585	T1586	cc	But,has
R2270	T5748	T5747	prep	of,analysis
R2271	T5749	T5750	amod	transverse,sections
R2272	T5750	T5748	pobj	sections,of
R2273	T5751	T5709	punct	.,failed
R2274	T5753	T5754	nsubj	We,examined
R2275	T5755	T5754	advmod	next,examined
R2276	T5756	T5757	det	the,establishment
R2277	T5757	T5754	dobj	establishment,examined
R2278	T5758	T5757	prep	of,establishment
R2279	T5759	T5760	amod	peripheral,projections
R228	T1587	T1588	mark	whether,is
R2280	T5760	T5758	pobj	projections,of
R2281	T5761	T5760	prep	upon,projections
R2282	T5762	T5763	amod	precocious,expression
R2283	T5763	T5761	pobj	expression,upon
R2284	T5764	T5765	compound	EWS,Pea3
R2285	T5765	T5763	compound	Pea3,expression
R2286	T5766	T5765	punct	-,Pea3
R2287	T5767	T5754	prep	in,examined
R2288	T5768	T5769	compound	DRG,neurons
R2289	T5769	T5767	pobj	neurons,in
R229	T1588	T1586	csubj	is,has
R2290	T5770	T5754	punct	.,examined
R2291	T5772	T5773	mark	While,reached
R2292	T5773	T5786	advcl	reached,established
R2293	T5774	T5775	amod	sensory,axons
R2294	T5775	T5773	nsubj	axons,reached
R2295	T5776	T5775	prep	in,axons
R2296	T5777	T5778	nmod	TauEWS,Isl1Cre
R2297	T5778	T5783	nmod	Isl1Cre,embryos
R2298	T5779	T5778	punct	-,Isl1Cre
R2299	T5780	T5778	nmod	Pea3,Isl1Cre
R230	T1589	T1590	det	the,expression
R2300	T5781	T5778	punct	/,Isl1Cre
R2301	T5782	T5778	punct	+,Isl1Cre
R2302	T5783	T5776	pobj	embryos,in
R2303	T5784	T5778	punct	/,Isl1Cre
R2304	T5785	T5778	punct	+,Isl1Cre
R2305	T5787	T5788	det	the,skin
R2306	T5788	T5773	dobj	skin,reached
R2307	T5789	T5773	cc	and,reached
R2308	T5790	T5773	conj	established,reached
R2309	T5791	T5792	amod	major,trunks
R231	T1590	T1588	nsubj	expression,is
R2310	T5792	T5790	dobj	trunks,established
R2311	T5793	T5792	compound	nerve,trunks
R2312	T5794	T5790	prep	by,established
R2313	T5795	T5794	pobj	E16.5,by
R2314	T5796	T5786	punct	", ",established
R2315	T5797	T5798	advmod	only,branching
R2316	T5798	T5786	nsubjpass	branching,established
R2317	T5799	T5798	amod	rudimentary,branching
R2318	T5800	T5801	amod	sensory,axon
R2319	T5801	T5798	compound	axon,branching
R232	T1591	T1590	amod	orderly,expression
R2320	T5802	T5786	auxpass	was,established
R2321	T5803	T5786	prep	within,established
R2322	T5804	T5805	det	the,skin
R2323	T5805	T5803	pobj	skin,within
R2324	T5806	T5807	punct	(,3D
R2325	T5807	T5786	parataxis	3D,established
R2326	T5808	T5807	compound	Figure,3D
R2327	T5809	T5807	cc	and,3D
R2328	T5810	T5807	conj	3J,3D
R2329	T5811	T5807	punct	),3D
R233	T1592	T1590	cc	and,expression
R2330	T5812	T5786	punct	.,established
R2331	T5814	T5815	prep	In,was
R2332	T5816	T5814	pobj	addition,In
R2333	T5817	T5815	punct	", ",was
R2334	T5818	T5815	expl	there,was
R2335	T5819	T5820	det	a,reduction
R2336	T5820	T5815	attr	reduction,was
R2337	T5821	T5820	amod	significant,reduction
R2338	T5822	T5820	prep	in,reduction
R2339	T5823	T5824	det	the,number
R234	T1593	T1590	conj	activity,expression
R2340	T5824	T5822	pobj	number,in
R2341	T5825	T5824	prep	of,number
R2342	T5826	T5827	compound	muscle,spindles
R2343	T5827	T5825	pobj	spindles,of
R2344	T5828	T5827	prep	in,spindles
R2345	T5829	T5830	nmod	TauEWS,Isl1Cre
R2346	T5830	T5835	nmod	Isl1Cre,embryos
R2347	T5831	T5830	punct	-,Isl1Cre
R2348	T5832	T5830	nmod	Pea3,Isl1Cre
R2349	T5833	T5830	punct	/,Isl1Cre
R235	T1594	T1590	prep	of,expression
R2350	T5834	T5830	punct	+,Isl1Cre
R2351	T5835	T5828	pobj	embryos,in
R2352	T5836	T5830	punct	/,Isl1Cre
R2353	T5837	T5830	punct	+,Isl1Cre
R2354	T5838	T5839	punct	(,%
R2355	T5839	T5824	parataxis	%,number
R2356	T5840	T5841	advmod	approximately,25
R2357	T5841	T5839	nummod	25,%
R2358	T5842	T5839	prep	of,%
R2359	T5843	T5844	amod	wild,type
R236	T1595	T1596	amod	transcriptional,programs
R2360	T5844	T5846	compound	type,complement
R2361	T5845	T5844	punct	-,type
R2362	T5846	T5842	pobj	complement,of
R2363	T5847	T5839	punct	;,%
R2364	T5848	T5849	nsubj	n,3
R2365	T5849	T5839	ccomp	3,%
R2366	T5850	T5849	punct	=,3
R2367	T5851	T5839	punct	),%
R2368	T5852	T5853	mark	as,assessed
R2369	T5853	T5815	advcl	assessed,was
R237	T1596	T1594	pobj	programs,of
R2370	T5854	T5853	prep	by,assessed
R2371	T5855	T5854	pobj	innervation,by
R2372	T5856	T5855	cc	and,innervation
R2373	T5857	T5855	conj	expression,innervation
R2374	T5858	T5855	prep	of,innervation
R2375	T5859	T5858	pobj	genes,of
R2376	T5860	T5859	amod	specific,genes
R2377	T5861	T5860	prep	for,specific
R2378	T5862	T5863	amod	intrafusal,fibers
R2379	T5863	T5861	pobj	fibers,for
R238	T1597	T1590	prep	at,expression
R2380	T5864	T5863	compound	muscle,fibers
R2381	T5865	T5866	amod	such,as
R2382	T5866	T5863	prep	as,fibers
R2383	T5867	T5866	pobj	Egr3,as
R2384	T5868	T5869	punct	(,26
R2385	T5869	T5853	parataxis	26,assessed
R2386	T5870	T5871	compound	Figure,3E
R2387	T5871	T5869	dep	3E,26
R2388	T5872	T5871	punct	", ",3E
R2389	T5873	T5871	conj	3F,3E
R239	T1598	T1599	advmod	much,later
R2390	T5874	T5873	punct	", ",3F
R2391	T5875	T5873	conj	3K,3F
R2392	T5876	T5875	punct	", ",3K
R2393	T5877	T5875	cc	and,3K
R2394	T5878	T5875	conj	3L,3K
R2395	T5879	T5869	punct	;,26
R2396	T5880	T5869	punct	[,26
R2397	T5881	T5869	punct	],26
R2398	T5882	T5869	punct	),26
R2399	T5883	T5815	punct	.,was
R240	T1599	T1600	amod	later,stages
R2400	T5885	T5886	prep	In,interfered
R2401	T5887	T5885	pobj	summary,In
R2402	T5888	T5886	punct	", ",interfered
R2403	T5889	T5890	mark	whereas,promoted
R2404	T5890	T5886	advcl	promoted,interfered
R2405	T5891	T5892	amod	isochronic,expression
R2406	T5892	T5890	nsubj	expression,promoted
R2407	T5893	T5892	prep	of,expression
R2408	T5894	T5895	compound	EWS,Pea3
R2409	T5895	T5893	pobj	Pea3,of
R241	T1600	T1597	pobj	stages,at
R2410	T5896	T5895	punct	-,Pea3
R2411	T5897	T5898	det	the,establishment
R2412	T5898	T5890	dobj	establishment,promoted
R2413	T5899	T5898	prep	of,establishment
R2414	T5900	T5901	amod	proprioceptive,projections
R2415	T5901	T5899	pobj	projections,of
R2416	T5902	T5901	amod	afferent,projections
R2417	T5903	T5901	prep	into,projections
R2418	T5904	T5905	det	the,cord
R2419	T5905	T5903	pobj	cord,into
R242	T1601	T1600	amod	developmental,stages
R2420	T5906	T5905	amod	ventral,cord
R2421	T5907	T5905	amod	spinal,cord
R2422	T5908	T5886	punct	", ",interfered
R2423	T5909	T5910	amod	precocious,expression
R2424	T5910	T5886	nsubj	expression,interfered
R2425	T5911	T5910	prep	of,expression
R2426	T5912	T5913	det	the,factor
R2427	T5913	T5911	pobj	factor,of
R2428	T5914	T5913	amod	same,factor
R2429	T5915	T5913	compound	ETS,factor
R243	T1602	T1590	punct	", ",expression
R2430	T5916	T5913	compound	signaling,factor
R2431	T5917	T5910	prep	in,expression
R2432	T5918	T5919	compound	DRG,neurons
R2433	T5919	T5917	pobj	neurons,in
R2434	T5920	T5886	prep	with,interfered
R2435	T5921	T5920	pobj	establishment,with
R2436	T5922	T5921	prep	of,establishment
R2437	T5923	T5922	pobj	projections,of
R2438	T5924	T5923	prep	into,projections
R2439	T5925	T5926	det	the,cord
R244	T1603	T1604	advmod	well,after
R2440	T5926	T5924	pobj	cord,into
R2441	T5927	T5926	amod	spinal,cord
R2442	T5928	T5929	advmod	as,as
R2443	T5929	T5924	cc	as,into
R2444	T5930	T5929	advmod	well,as
R2445	T5931	T5924	conj	to,into
R2446	T5932	T5933	amod	peripheral,targets
R2447	T5933	T5931	pobj	targets,to
R2448	T5934	T5886	punct	.,interfered
R245	T1604	T1590	prep	after,expression
R2451	T6663	T6664	amod	Precocious,Expression
R2452	T6664	T6668	nsubj	Expression,Promotes
R2453	T6665	T6666	compound	EWS,Pea3
R2454	T6666	T6664	compound	Pea3,Expression
R2455	T6667	T6666	punct	-,Pea3
R2456	T6669	T6670	npadvmod	Neurotrophin,Independent
R2457	T6670	T6672	amod	Independent,Survival
R2458	T6671	T6670	punct	-,Independent
R2459	T6672	T6668	dobj	Survival,Promotes
R246	T1605	T1606	compound	cell,cycle
R2460	T6673	T6672	cc	and,Survival
R2461	T6674	T6675	compound	Neurite,Outgrowth
R2462	T6675	T6672	conj	Outgrowth,Survival
R2463	T6677	T6678	aux	To,begin
R2464	T6678	T6679	advcl	begin,turned
R2465	T6680	T6681	aux	to,address
R2466	T6681	T6678	xcomp	address,begin
R2467	T6682	T6683	det	the,mechanisms
R2468	T6683	T6681	dobj	mechanisms,address
R2469	T6684	T6683	amod	cellular,mechanisms
R247	T1606	T1607	compound	cycle,exit
R2470	T6685	T6684	cc	and,cellular
R2471	T6686	T6684	conj	molecular,cellular
R2472	T6687	T6683	acl	involved,mechanisms
R2473	T6688	T6687	prep	in,involved
R2474	T6689	T6690	det	the,actions
R2475	T6690	T6688	pobj	actions,in
R2476	T6691	T6690	amod	distinct,actions
R2477	T6692	T6690	amod	biological,actions
R2478	T6693	T6690	prep	of,actions
R2479	T6694	T6695	compound	EWS,Pea3
R248	T1607	T1604	pobj	exit,after
R2480	T6695	T6693	pobj	Pea3,of
R2481	T6696	T6695	punct	-,Pea3
R2482	T6697	T6687	prep	at,involved
R2483	T6698	T6699	amod	different,stages
R2484	T6699	T6697	pobj	stages,at
R2485	T6700	T6699	amod	developmental,stages
R2486	T6701	T6679	punct	", ",turned
R2487	T6702	T6679	nsubj	we,turned
R2488	T6703	T6679	advmod	first,turned
R2489	T6704	T6679	prep	to,turned
R249	T1608	T1588	punct	", ",is
R2490	T6705	T6706	advmod	in,vitro
R2491	T6706	T6707	amod	vitro,experiments
R2492	T6707	T6704	pobj	experiments,to
R2493	T6708	T6707	compound	culture,experiments
R2494	T6709	T6679	punct	.,turned
R2495	T6711	T6712	det	These,experiments
R2496	T6712	T6713	nsubj	experiments,permit
R2497	T6714	T6713	dobj	assessment,permit
R2498	T6715	T6714	prep	of,assessment
R2499	T6716	T6717	mark	whether,influences
R250	T1609	T1610	det	an,step
R2500	T6717	T6715	pcomp	influences,of
R2501	T6718	T6719	amod	precocious,signaling
R2502	T6719	T6717	nsubj	signaling,influences
R2503	T6720	T6719	compound	ETS,signaling
R2504	T6721	T6722	compound	transcription,factor
R2505	T6722	T6719	compound	factor,signaling
R2506	T6723	T6724	amod	neuronal,survival
R2507	T6724	T6717	dobj	survival,influences
R2508	T6725	T6724	cc	and,survival
R2509	T6726	T6727	nmod	in,vitro
R251	T1610	T1588	attr	step,is
R2510	T6727	T6728	nmod	vitro,outgrowth
R2511	T6728	T6724	conj	outgrowth,survival
R2512	T6729	T6728	compound	neurite,outgrowth
R2513	T6730	T6724	prep	of,survival
R2514	T6731	T6732	compound	DRG,neurons
R2515	T6732	T6730	pobj	neurons,of
R2516	T6733	T6724	punct	", ",survival
R2517	T6734	T6735	nummod	two,parameters
R2518	T6735	T6724	appos	parameters,survival
R2519	T6736	T6737	advmod	prominently,influenced
R252	T1611	T1610	amod	essential,step
R2520	T6737	T6735	acl	influenced,parameters
R2521	T6738	T6737	agent	by,influenced
R2522	T6739	T6740	npadvmod	target,derived
R2523	T6740	T6742	amod	derived,factors
R2524	T6741	T6740	punct	-,derived
R2525	T6742	T6738	pobj	factors,by
R2526	T6743	T6742	amod	neurotrophic,factors
R2527	T6744	T6742	cc	and,factors
R2528	T6745	T6746	poss	their,receptors
R2529	T6746	T6742	conj	receptors,factors
R253	T1612	T1610	prep	in,step
R2530	T6747	T6713	punct	.,permit
R2531	T6749	T6750	nsubj	We,cultured
R2532	T6751	T6752	nmod	E13.5,explants
R2533	T6752	T6750	dobj	explants,cultured
R2534	T6753	T6752	amod	whole,explants
R2535	T6754	T6752	compound	DRG,explants
R2536	T6755	T6752	prep	from,explants
R2537	T6756	T6757	amod	wild,type
R2538	T6757	T6759	nmod	type,embryos
R2539	T6758	T6757	punct	-,type
R254	T1613	T1614	det	the,progression
R2540	T6759	T6755	pobj	embryos,from
R2541	T6760	T6757	cc	and,type
R2542	T6761	T6762	nmod	TauEWS,Isl1Cre
R2543	T6762	T6757	conj	Isl1Cre,type
R2544	T6763	T6762	punct	-,Isl1Cre
R2545	T6764	T6762	nmod	Pea3,Isl1Cre
R2546	T6765	T6762	punct	/,Isl1Cre
R2547	T6766	T6762	punct	+,Isl1Cre
R2548	T6767	T6762	punct	/,Isl1Cre
R2549	T6768	T6762	punct	+,Isl1Cre
R255	T1614	T1612	pobj	progression,in
R2550	T6769	T6752	prep	in,explants
R2551	T6770	T6771	det	the,presence
R2552	T6771	T6769	pobj	presence,in
R2553	T6772	T6771	prep	of,presence
R2554	T6773	T6772	pobj	NGF,of
R2555	T6774	T6773	cc	or,NGF
R2556	T6775	T6773	conj	NT,NGF
R2557	T6776	T6775	punct	-,NT
R2558	T6777	T6775	nummod	3,NT
R2559	T6778	T6769	cc	or,in
R256	T1615	T1614	prep	of,progression
R2560	T6779	T6769	conj	in,in
R2561	T6780	T6781	det	the,absence
R2562	T6781	T6779	pobj	absence,in
R2563	T6782	T6781	prep	of,absence
R2564	T6783	T6782	pobj	neurotrophins,of
R2565	T6784	T6750	cc	and,cultured
R2566	T6785	T6750	conj	analyzed,cultured
R2567	T6786	T6787	amod	neuronal,survival
R2568	T6787	T6785	dobj	survival,analyzed
R2569	T6788	T6787	cc	and,survival
R257	T1616	T1617	amod	neuronal,differentiation
R2570	T6789	T6790	compound	neurite,outgrowth
R2571	T6790	T6787	conj	outgrowth,survival
R2572	T6791	T6785	prep	on,analyzed
R2573	T6792	T6793	compound	matrigel,substrate
R2574	T6793	T6791	pobj	substrate,on
R2575	T6794	T6785	prep	after,analyzed
R2576	T6795	T6796	nummod	48,h
R2577	T6796	T6794	pobj	h,after
R2578	T6797	T6798	advmod	in,vitro
R2579	T6798	T6785	advmod	vitro,analyzed
R258	T1617	T1615	pobj	differentiation,of
R2580	T6799	T6750	punct	.,cultured
R2581	T6801	T6802	prep	Without,survived
R2582	T6803	T6804	amod	neurotrophic,support
R2583	T6804	T6801	pobj	support,Without
R2584	T6805	T6802	punct	", ",survived
R2585	T6806	T6807	advmod	very,few
R2586	T6807	T6808	amod	few,neurons
R2587	T6808	T6802	nsubj	neurons,survived
R2588	T6809	T6810	amod	wild,type
R2589	T6810	T6808	compound	type,neurons
R259	T1618	T1617	cc	and,differentiation
R2590	T6811	T6810	punct	-,type
R2591	T6812	T6808	compound	DRG,neurons
R2592	T6813	T6814	punct	(,4A
R2593	T6814	T6802	parataxis	4A,survived
R2594	T6815	T6814	compound	Figure,4A
R2595	T6816	T6814	punct	),4A
R2596	T6817	T6802	punct	.,survived
R2597	T6819	T6820	prep	In,led
R2598	T6821	T6819	pobj	contrast,In
R2599	T6822	T6820	punct	", ",led
R260	T1619	T1620	compound	circuit,assembly
R2600	T6823	T6820	csubj	culturing,led
R2601	T6824	T6825	amod	wild,type
R2602	T6825	T6827	compound	type,DRG
R2603	T6826	T6825	punct	-,type
R2604	T6827	T6823	dobj	DRG,culturing
R2605	T6828	T6823	prep	with,culturing
R2606	T6829	T6830	amod	neurotrophic,factors
R2607	T6830	T6828	pobj	factors,with
R2608	T6831	T6820	prep	to,led
R2609	T6832	T6833	amod	neuronal,survival
R261	T1620	T1617	conj	assembly,differentiation
R2610	T6833	T6831	pobj	survival,to
R2611	T6834	T6833	cc	and,survival
R2612	T6835	T6836	compound	neurite,outgrowth
R2613	T6836	T6833	conj	outgrowth,survival
R2614	T6837	T6820	punct	.,led
R2615	T6839	T6840	nsubj	Addition,resulted
R2616	T6841	T6839	prep	of,Addition
R2617	T6842	T6841	pobj	NGF,of
R2618	T6843	T6842	punct	", ",NGF
R2619	T6844	T6845	dep	which,supports
R262	T1621	T1586	advmod	yet,has
R2620	T6845	T6842	relcl	supports,NGF
R2621	T6846	T6845	dobj	survival,supports
R2622	T6847	T6846	prep	of,survival
R2623	T6848	T6849	amod	cutaneous,afferents
R2624	T6849	T6847	pobj	afferents,of
R2625	T6850	T6840	punct	", ",resulted
R2626	T6851	T6840	prep	in,resulted
R2627	T6852	T6853	amod	straight,outgrowth
R2628	T6853	T6851	pobj	outgrowth,in
R2629	T6854	T6852	cc	and,straight
R263	T1622	T1623	aux	to,resolved
R2630	T6855	T6852	conj	unbranched,straight
R2631	T6856	T6853	compound	neurite,outgrowth
R2632	T6857	T6858	punct	(,4B
R2633	T6858	T6853	parataxis	4B,outgrowth
R2634	T6859	T6858	compound	Figure,4B
R2635	T6860	T6858	punct	),4B
R2636	T6861	T6840	punct	", ",resulted
R2637	T6862	T6863	mark	while,resulted
R2638	T6863	T6840	advcl	resulted,resulted
R2639	T6864	T6863	nsubj	cultures,resulted
R264	T1623	T1586	xcomp	resolved,has
R2640	T6865	T6864	acl	grown,cultures
R2641	T6866	T6865	prep	in,grown
R2642	T6867	T6868	det	the,presence
R2643	T6868	T6866	pobj	presence,in
R2644	T6869	T6868	prep	of,presence
R2645	T6870	T6869	pobj	NT,of
R2646	T6871	T6870	punct	-,NT
R2647	T6872	T6870	nummod	3,NT
R2648	T6873	T6870	punct	", ",NT
R2649	T6874	T6875	dep	which,supports
R265	T1624	T1623	auxpass	be,resolved
R2650	T6875	T6870	relcl	supports,NT
R2651	T6876	T6875	dobj	survival,supports
R2652	T6877	T6876	prep	of,survival
R2653	T6878	T6879	amod	proprioceptive,afferents
R2654	T6879	T6877	pobj	afferents,of
R2655	T6880	T6863	punct	", ",resulted
R2656	T6881	T6863	prep	in,resulted
R2657	T6882	T6883	det	a,pattern
R2658	T6883	T6881	pobj	pattern,in
R2659	T6884	T6885	advmod	highly,branched
R266	T1625	T1586	punct	.,has
R2660	T6885	T6883	amod	branched,pattern
R2661	T6886	T6887	compound	neurite,outgrowth
R2662	T6887	T6883	compound	outgrowth,pattern
R2663	T6888	T6863	prep	after,resulted
R2664	T6889	T6890	nummod	48,h
R2665	T6890	T6888	pobj	h,after
R2666	T6891	T6892	advmod	in,vitro
R2667	T6892	T6890	advmod	vitro,h
R2668	T6893	T6894	punct	(,4C
R2669	T6894	T6863	parataxis	4C,resulted
R267	T1627	T1628	det	The,differentiation
R2670	T6895	T6894	compound	Figure,4C
R2671	T6896	T6894	punct	),4C
R2672	T6897	T6840	punct	.,resulted
R2673	T6899	T6900	advmod	Surprisingly,survived
R2674	T6901	T6900	punct	", ",survived
R2675	T6902	T6903	compound	DRG,neurons
R2676	T6903	T6900	nsubj	neurons,survived
R2677	T6904	T6903	acl	isolated,neurons
R2678	T6905	T6904	prep	from,isolated
R2679	T6906	T6907	nmod	TauEWS,Isl1Cre
R268	T1628	T1629	nsubjpass	differentiation,studied
R2680	T6907	T6912	nmod	Isl1Cre,embryos
R2681	T6908	T6907	punct	-,Isl1Cre
R2682	T6909	T6907	nmod	Pea3,Isl1Cre
R2683	T6910	T6907	punct	/,Isl1Cre
R2684	T6911	T6907	punct	+,Isl1Cre
R2685	T6912	T6905	pobj	embryos,from
R2686	T6913	T6907	punct	/,Isl1Cre
R2687	T6914	T6907	punct	+,Isl1Cre
R2688	T6915	T6904	cc	and,isolated
R2689	T6916	T6904	conj	cultured,isolated
R269	T1630	T1628	prep	of,differentiation
R2690	T6917	T6916	prep	without,cultured
R2691	T6918	T6919	amod	neurotrophic,support
R2692	T6919	T6917	pobj	support,without
R2693	T6920	T6900	prep	after,survived
R2694	T6921	T6922	nummod	48,h
R2695	T6922	T6920	pobj	h,after
R2696	T6923	T6924	advmod	in,vitro
R2697	T6924	T6922	advmod	vitro,h
R2698	T6925	T6900	cc	and,survived
R2699	T6926	T6927	aux	had,established
R270	T1631	T1632	amod	sensory,neurons
R2700	T6927	T6900	conj	established,survived
R2701	T6928	T6929	amod	long,neurites
R2702	T6929	T6927	dobj	neurites,established
R2703	T6930	T6928	cc	and,long
R2704	T6931	T6932	advmod	highly,branched
R2705	T6932	T6928	conj	branched,long
R2706	T6933	T6934	punct	(,4D
R2707	T6934	T6927	parataxis	4D,established
R2708	T6935	T6934	compound	Figure,4D
R2709	T6936	T6934	punct	),4D
R271	T1632	T1630	pobj	neurons,of
R2710	T6937	T6900	punct	.,survived
R2711	T6939	T6940	preconj	Neither,pattern
R2712	T6940	T6942	nsubj	pattern,changed
R2713	T6941	T6940	det	the,pattern
R2714	T6943	T6940	prep	of,pattern
R2715	T6944	T6945	compound	neurite,outgrowth
R2716	T6945	T6943	pobj	outgrowth,of
R2717	T6946	T6940	cc	nor,pattern
R2718	T6947	T6948	amod	neuronal,survival
R2719	T6948	T6940	conj	survival,pattern
R272	T1633	T1628	prep	of,differentiation
R2720	T6949	T6942	advmod	significantly,changed
R2721	T6950	T6942	prep	after,changed
R2722	T6951	T6950	pobj	application,after
R2723	T6952	T6951	prep	of,application
R2724	T6953	T6954	preconj	either,NGF
R2725	T6954	T6952	pobj	NGF,of
R2726	T6955	T6954	cc	or,NGF
R2727	T6956	T6954	conj	NT,NGF
R2728	T6957	T6956	punct	-,NT
R2729	T6958	T6956	nummod	3,NT
R273	T1634	T1635	amod	dorsal,ganglia
R2730	T6959	T6960	punct	(,4E
R2731	T6960	T6942	parataxis	4E,changed
R2732	T6961	T6960	compound	Figure,4E
R2733	T6962	T6960	cc	and,4E
R2734	T6963	T6960	conj	4F,4E
R2735	T6964	T6960	punct	),4E
R2736	T6965	T6942	punct	.,changed
R2737	T6967	T6968	aux	To,compare
R2738	T6968	T6970	advcl	compare,generated
R2739	T6969	T6968	advmod	directly,compare
R274	T1635	T1633	pobj	ganglia,of
R2740	T6971	T6972	compound	neurotrophin,dependence
R2741	T6972	T6968	dobj	dependence,compare
R2742	T6973	T6972	prep	of,dependence
R2743	T6974	T6975	compound	DRG,neurons
R2744	T6975	T6973	pobj	neurons,of
R2745	T6976	T6975	acl	expressing,neurons
R2746	T6977	T6978	compound	EWS,Pea3
R2747	T6978	T6976	dobj	Pea3,expressing
R2748	T6979	T6978	punct	-,Pea3
R2749	T6980	T6978	prep	from,Pea3
R275	T1636	T1635	compound	root,ganglia
R2750	T6981	T6982	det	the,locus
R2751	T6982	T6980	pobj	locus,from
R2752	T6983	T6982	compound	Tau,locus
R2753	T6984	T6982	prep	at,locus
R2754	T6985	T6986	det	a,time
R2755	T6986	T6984	pobj	time,at
R2756	T6987	T6986	amod	precocious,time
R2757	T6988	T6987	cc	versus,precocious
R2758	T6989	T6987	conj	isochronic,precocious
R2759	T6990	T6986	prep	of,time
R276	T1637	T1635	punct	(,ganglia
R2760	T6991	T6990	pobj	onset,of
R2761	T6992	T6970	punct	", ",generated
R2762	T6993	T6970	nsubj	we,generated
R2763	T6994	T6995	det	a,strain
R2764	T6995	T6970	dobj	strain,generated
R2765	T6996	T6995	prep	of,strain
R2766	T6997	T6996	pobj	mice,of
R2767	T6998	T6999	prep	in,expressed
R2768	T6999	T6995	relcl	expressed,strain
R2769	T7000	T6998	pobj	which,in
R277	T1638	T1635	appos	DRG,ganglia
R2770	T7001	T7002	compound	Cre,recombinase
R2771	T7002	T6999	nsubjpass	recombinase,expressed
R2772	T7003	T6999	auxpass	is,expressed
R2773	T7004	T6999	prep	from,expressed
R2774	T7005	T7006	det	the,locus
R2775	T7006	T7004	pobj	locus,from
R2776	T7007	T7006	compound	PV,locus
R2777	T7008	T7009	punct	(,S4
R2778	T7009	T6970	parataxis	S4,generated
R2779	T7010	T7009	compound	Figure,S4
R278	T1639	T1629	punct	),studied
R2780	T7011	T7009	punct	),S4
R2781	T7012	T6970	punct	.,generated
R2782	T7014	T7015	det	The,expression
R2783	T7015	T7016	nsubjpass	expression,restricted
R2784	T7017	T7015	prep	of,expression
R2785	T7018	T7017	pobj	GFP,of
R2786	T7019	T7015	prep	in,expression
R2787	T7020	T7021	nmod	TaumGFP,PVCre
R2788	T7021	T7019	pobj	PVCre,in
R2789	T7022	T7021	punct	/,PVCre
R279	T1640	T1629	aux	has,studied
R2790	T7023	T7021	punct	+,PVCre
R2791	T7024	T7021	punct	/,PVCre
R2792	T7025	T7021	punct	+,PVCre
R2793	T7026	T7016	auxpass	was,restricted
R2794	T7027	T7016	prep	to,restricted
R2795	T7028	T7029	nmod	PV,neurons
R2796	T7029	T7027	pobj	neurons,to
R2797	T7030	T7028	punct	+,PV
R2798	T7031	T7029	amod	proprioceptive,neurons
R2799	T7032	T7029	compound	DRG,neurons
R280	T1641	T1629	auxpass	been,studied
R2800	T7033	T7016	cc	and,restricted
R2801	T7034	T7016	conj	mirrored,restricted
R2802	T7035	T7036	det	the,onset
R2803	T7036	T7034	dobj	onset,mirrored
R2804	T7037	T7036	prep	of,onset
R2805	T7038	T7037	pobj	expression,of
R2806	T7039	T7038	prep	of,expression
R2807	T7040	T7039	pobj	PV,of
R2808	T7041	T7034	prep	at,mirrored
R2809	T7042	T7043	advmod	approximately,E14
R281	T1642	T1629	advmod	extensively,studied
R2810	T7043	T7041	pobj	E14,at
R2811	T7044	T7045	punct	(,S4
R2812	T7045	T7043	parataxis	S4,E14
R2813	T7046	T7045	compound	Figure,S4
R2814	T7047	T7045	punct	;,S4
R2815	T7048	T7049	nsubj	data,shown
R2816	T7049	T7045	ccomp	shown,S4
R2817	T7050	T7049	neg	not,shown
R2818	T7051	T7045	punct	),S4
R2819	T7052	T7016	punct	.,restricted
R282	T1643	T1629	prep	with,studied
R2820	T7054	T7055	nsubj	We,cultured
R2821	T7056	T7055	advmod	next,cultured
R2822	T7057	T7058	nmod	E14.5,explants
R2823	T7058	T7055	dobj	explants,cultured
R2824	T7059	T7058	amod	whole,explants
R2825	T7060	T7058	compound	DRG,explants
R2826	T7061	T7058	prep	from,explants
R2827	T7062	T7063	nmod	TauEWS,PVCre
R2828	T7063	T7068	nmod	PVCre,mice
R2829	T7064	T7063	punct	-,PVCre
R283	T1644	T1643	pobj	respect,with
R2830	T7065	T7063	nmod	Pea3,PVCre
R2831	T7066	T7063	punct	/,PVCre
R2832	T7067	T7063	punct	+,PVCre
R2833	T7068	T7061	pobj	mice,from
R2834	T7069	T7063	punct	/,PVCre
R2835	T7070	T7063	punct	+,PVCre
R2836	T7071	T7063	cc	and,PVCre
R2837	T7072	T7073	nmod	TaumGFP,PVCre
R2838	T7073	T7063	conj	PVCre,PVCre
R2839	T7074	T7073	punct	/,PVCre
R284	T1645	T1644	prep	to,respect
R2840	T7075	T7073	punct	+,PVCre
R2841	T7076	T7073	punct	/,PVCre
R2842	T7077	T7073	punct	+,PVCre
R2843	T7078	T7055	prep	for,cultured
R2844	T7079	T7080	nummod	48,h
R2845	T7080	T7078	pobj	h,for
R2846	T7081	T7082	advmod	in,vitro
R2847	T7082	T7080	advmod	vitro,h
R2848	T7083	T7055	prep	in,cultured
R2849	T7084	T7085	det	the,presence
R285	T1646	T1647	amod	inductive,events
R2850	T7085	T7083	pobj	presence,in
R2851	T7086	T7085	cc	or,presence
R2852	T7087	T7085	conj	absence,presence
R2853	T7088	T7085	prep	of,presence
R2854	T7089	T7088	pobj	NT,of
R2855	T7090	T7089	punct	-,NT
R2856	T7091	T7089	nummod	3,NT
R2857	T7092	T7093	punct	(,Figure
R2858	T7093	T7055	parataxis	Figure,cultured
R2859	T7094	T7093	nummod	5,Figure
R286	T1647	T1645	pobj	events,to
R2860	T7095	T7093	punct	),Figure
R2861	T7096	T7055	punct	.,cultured
R2862	T7098	T7099	nsubj	We,found
R2863	T7100	T7101	mark	that,survived
R2864	T7101	T7099	ccomp	survived,found
R2865	T7102	T7103	compound	DRG,neurons
R2866	T7103	T7101	nsubj	neurons,survived
R2867	T7104	T7103	prep	from,neurons
R2868	T7105	T7106	det	both,genotypes
R2869	T7106	T7104	pobj	genotypes,from
R287	T1648	T1649	dep	that,specify
R2870	T7107	T7101	cc	and,survived
R2871	T7108	T7101	conj	extended,survived
R2872	T7109	T7108	dobj	neurites,extended
R2873	T7110	T7111	advmod	only,in
R2874	T7111	T7101	prep	in,survived
R2875	T7112	T7113	det	the,presence
R2876	T7113	T7111	pobj	presence,in
R2877	T7114	T7113	prep	of,presence
R2878	T7115	T7114	pobj	NT,of
R2879	T7116	T7115	punct	-,NT
R288	T1649	T1647	relcl	specify,events
R2880	T7117	T7115	nummod	3,NT
R2881	T7118	T7101	punct	", ",survived
R2882	T7119	T7120	mark	whereas,died
R2883	T7120	T7101	advcl	died,survived
R2884	T7121	T7120	nsubj	they,died
R2885	T7122	T7120	prep	in,died
R2886	T7123	T7124	det	the,absence
R2887	T7124	T7122	pobj	absence,in
R2888	T7125	T7124	prep	of,absence
R2889	T7126	T7125	pobj	NT,of
R289	T1650	T1651	amod	neuronal,fate
R2890	T7127	T7126	punct	-,NT
R2891	T7128	T7126	nummod	3,NT
R2892	T7129	T7130	punct	(,Figure
R2893	T7130	T7101	parataxis	Figure,survived
R2894	T7131	T7130	nummod	5,Figure
R2895	T7132	T7130	punct	),Figure
R2896	T7133	T7099	punct	.,found
R2897	T7135	T7136	advmod	Together,suggest
R2898	T7137	T7136	punct	", ",suggest
R2899	T7138	T7139	det	these,findings
R290	T1651	T1649	dobj	fate,specify
R2900	T7139	T7136	nsubj	findings,suggest
R2901	T7140	T7141	mark	that,is
R2902	T7141	T7136	ccomp	is,suggest
R2903	T7142	T7143	advmod	only,signaling
R2904	T7143	T7141	nsubj	signaling,is
R2905	T7144	T7143	amod	precocious,signaling
R2906	T7145	T7144	cc	but,precocious
R2907	T7146	T7145	neg	not,but
R2908	T7147	T7144	conj	isochronic,precocious
R2909	T7148	T7143	compound	ETS,signaling
R291	T1652	T1653	punct	[,3
R2910	T7149	T7143	prep	in,signaling
R2911	T7150	T7151	compound	DRG,neurons
R2912	T7151	T7149	pobj	neurons,in
R2913	T7152	T7141	acomp	capable,is
R2914	T7153	T7152	prep	of,capable
R2915	T7154	T7153	pcomp	uncoupling,of
R2916	T7155	T7154	dobj	survival,uncoupling
R2917	T7156	T7155	cc	and,survival
R2918	T7157	T7158	compound	neurite,outgrowth
R2919	T7158	T7155	conj	outgrowth,survival
R292	T1653	T1651	parataxis	3,fate
R2920	T7159	T7154	prep	from,uncoupling
R2921	T7160	T7161	det	a,requirement
R2922	T7161	T7159	pobj	requirement,from
R2923	T7162	T7161	prep	for,requirement
R2924	T7163	T7164	compound	neurotrophin,signaling
R2925	T7164	T7162	pobj	signaling,for
R2926	T7165	T7166	advmod	normally,observed
R2927	T7166	T7161	acl	observed,requirement
R2928	T7167	T7166	prep	in,observed
R2929	T7168	T7169	amod	wild,type
R293	T1654	T1653	nummod	2,3
R2930	T7169	T7171	compound	type,DRG
R2931	T7170	T7169	punct	-,type
R2932	T7171	T7167	pobj	DRG,in
R2933	T7172	T7136	punct	.,suggest
R2934	T7174	T7175	aux	To,determine
R2935	T7175	T7176	advcl	determine,analyzed
R2936	T7177	T7178	mark	whether,is
R2937	T7178	T7175	ccomp	is,determine
R2938	T7179	T7180	amod	neuronal,survival
R2939	T7180	T7178	nsubj	survival,is
R294	T1655	T1653	punct	",",3
R2940	T7181	T7180	prep	of,survival
R2941	T7182	T7183	compound	DRG,neurons
R2942	T7183	T7181	pobj	neurons,of
R2943	T7184	T7183	prep	from,neurons
R2944	T7185	T7186	nmod	TauEWS,Isl1Cre
R2945	T7186	T7191	nmod	Isl1Cre,embryos
R2946	T7187	T7186	punct	-,Isl1Cre
R2947	T7188	T7186	nmod	Pea3,Isl1Cre
R2948	T7189	T7186	punct	/,Isl1Cre
R2949	T7190	T7186	punct	+,Isl1Cre
R295	T1656	T1653	punct	],3
R2950	T7191	T7184	pobj	embryos,from
R2951	T7192	T7186	punct	/,Isl1Cre
R2952	T7193	T7186	punct	+,Isl1Cre
R2953	T7194	T7180	prep	in,survival
R2954	T7195	T7196	det	the,absence
R2955	T7196	T7194	pobj	absence,in
R2956	T7197	T7196	prep	of,absence
R2957	T7198	T7199	amod	neurotrophic,support
R2958	T7199	T7197	pobj	support,of
R2959	T7200	T7178	acomp	sufficient,is
R296	T1657	T1651	punct	", ",fate
R2960	T7201	T7202	aux	to,explain
R2961	T7202	T7200	xcomp	explain,sufficient
R2962	T7203	T7204	det	the,outgrowth
R2963	T7204	T7202	dobj	outgrowth,explain
R2964	T7205	T7204	amod	observed,outgrowth
R2965	T7206	T7204	amod	neuronal,outgrowth
R2966	T7207	T7176	punct	", ",analyzed
R2967	T7208	T7176	nsubj	we,analyzed
R2968	T7209	T7176	dobj	DRG,analyzed
R2969	T7210	T7209	acl	isolated,DRG
R297	T1658	T1659	advmod	as,as
R2970	T7211	T7210	prep	from,isolated
R2971	T7212	T7213	compound	mice,mutant
R2972	T7213	T7211	pobj	mutant,from
R2973	T7214	T7176	prep	in,analyzed
R2974	T7215	T7216	det	the,Bax
R2975	T7216	T7214	pobj	Bax,in
R2976	T7217	T7216	amod	proapoptotic,Bax
R2977	T7218	T7216	compound	gene,Bax
R2978	T7219	T7220	punct	[,27
R2979	T7220	T7176	parataxis	27,analyzed
R298	T1659	T1651	cc	as,fate
R2980	T7221	T7220	punct	],27
R2981	T7222	T7176	punct	.,analyzed
R2982	T7224	T7225	advcl	Consistent,survived
R2983	T7226	T7224	prep	with,Consistent
R2984	T7227	T7228	amod	previous,results
R2985	T7228	T7226	pobj	results,with
R2986	T7229	T7225	punct	", ",survived
R2987	T7230	T7231	nmod	Bax,neurons
R2988	T7231	T7225	nsubj	neurons,survived
R2989	T7232	T7230	punct	−,Bax
R299	T1660	T1659	advmod	well,as
R2990	T7233	T7230	punct	/,Bax
R2991	T7234	T7230	punct	−,Bax
R2992	T7235	T7231	compound	DRG,neurons
R2993	T7236	T7225	prep	without,survived
R2994	T7237	T7238	amod	neurotrophic,support
R2995	T7238	T7236	pobj	support,without
R2996	T7239	T7240	punct	[,28
R2997	T7240	T7225	parataxis	28,survived
R2998	T7241	T7240	punct	],28
R2999	T7242	T7225	punct	.,survived
R300	T1661	T1662	det	the,involvement
R3000	T7244	T7245	prep	In,was
R3001	T7246	T7244	pobj	contrast,In
R3002	T7247	T7245	punct	", ",was
R3003	T7248	T7249	compound	neurite,outgrowth
R3004	T7249	T7245	nsubj	outgrowth,was
R3005	T7250	T7249	prep	of,outgrowth
R3006	T7251	T7252	nmod	Bax,neurons
R3007	T7252	T7250	pobj	neurons,of
R3008	T7253	T7251	punct	−,Bax
R3009	T7254	T7251	punct	/,Bax
R301	T1662	T1651	conj	involvement,fate
R3010	T7255	T7251	punct	−,Bax
R3011	T7256	T7252	compound	DRG,neurons
R3012	T7257	T7258	advmod	significantly,less
R3013	T7258	T7245	acomp	less,was
R3014	T7259	T7260	punct	(,see
R3015	T7260	T7258	parataxis	see,less
R3016	T7261	T7262	compound	Figure,4G
R3017	T7262	T7260	dobj	4G,see
R3018	T7263	T7260	punct	),see
R3019	T7264	T7258	prep	than,less
R302	T1663	T1662	prep	of,involvement
R3020	T7265	T7264	pobj	that,than
R3021	T7266	T7265	prep	of,that
R3022	T7267	T7268	preconj	either,DRG
R3023	T7268	T7266	pobj	DRG,of
R3024	T7269	T7268	prep	from,DRG
R3025	T7270	T7271	nmod	TauEWS,Isl1Cre
R3026	T7271	T7276	nmod	Isl1Cre,embryos
R3027	T7272	T7271	punct	-,Isl1Cre
R3028	T7273	T7271	nmod	Pea3,Isl1Cre
R3029	T7274	T7271	punct	/,Isl1Cre
R303	T1664	T1665	amod	late,factors
R3030	T7275	T7271	punct	+,Isl1Cre
R3031	T7276	T7269	pobj	embryos,from
R3032	T7277	T7271	punct	/,Isl1Cre
R3033	T7278	T7271	punct	+,Isl1Cre
R3034	T7279	T7268	acl	cultured,DRG
R3035	T7280	T7279	prep	in,cultured
R3036	T7281	T7282	det	the,absence
R3037	T7282	T7280	pobj	absence,in
R3038	T7283	T7282	prep	of,absence
R3039	T7284	T7285	amod	neurotrophic,support
R304	T1665	T1663	pobj	factors,of
R3040	T7285	T7283	pobj	support,of
R3041	T7286	T7287	punct	(,see
R3042	T7287	T7279	parataxis	see,cultured
R3043	T7288	T7289	compound	Figure,4D
R3044	T7289	T7287	dobj	4D,see
R3045	T7290	T7287	punct	),see
R3046	T7291	T7268	cc	or,DRG
R3047	T7292	T7293	nmod	Bax,neurons
R3048	T7293	T7268	conj	neurons,DRG
R3049	T7294	T7292	punct	−,Bax
R305	T1666	T1667	npadvmod	target,derived
R3050	T7295	T7292	punct	/,Bax
R3051	T7296	T7292	punct	−,Bax
R3052	T7297	T7293	compound	DRG,neurons
R3053	T7298	T7293	acl	cultured,neurons
R3054	T7299	T7298	prep	in,cultured
R3055	T7300	T7301	det	the,presence
R3056	T7301	T7299	pobj	presence,in
R3057	T7302	T7301	prep	of,presence
R3058	T7303	T7304	amod	neurotrophic,support
R3059	T7304	T7302	pobj	support,of
R306	T1667	T1665	amod	derived,factors
R3060	T7305	T7306	punct	(,see
R3061	T7306	T7298	parataxis	see,cultured
R3062	T7307	T7308	compound	Figure,4H
R3063	T7308	T7306	dobj	4H,see
R3064	T7309	T7308	cc	and,4H
R3065	T7310	T7308	conj	4I,4H
R3066	T7311	T7306	punct	),see
R3067	T7312	T7245	punct	.,was
R3068	T7314	T7315	det	These,findings
R3069	T7315	T7316	nsubj	findings,suggest
R307	T1668	T1667	punct	-,derived
R3070	T7317	T7318	mark	that,promotes
R3071	T7318	T7316	ccomp	promotes,suggest
R3072	T7319	T7318	prep	in,promotes
R3073	T7320	T7319	pobj	addition,in
R3074	T7321	T7320	prep	to,addition
R3075	T7322	T7321	pcomp	mediating,to
R3076	T7323	T7324	npadvmod	neurotrophin,independent
R3077	T7324	T7326	amod	independent,survival
R3078	T7325	T7324	punct	-,independent
R3079	T7326	T7322	dobj	survival,mediating
R308	T1669	T1665	amod	neurotrophic,factors
R3080	T7327	T7326	amod	neuronal,survival
R3081	T7328	T7318	punct	", ",promotes
R3082	T7329	T7318	nsubj	expression,promotes
R3083	T7330	T7329	prep	of,expression
R3084	T7331	T7332	compound	EWS,Pea3
R3085	T7332	T7330	pobj	Pea3,of
R3086	T7333	T7332	punct	-,Pea3
R3087	T7334	T7329	prep	in,expression
R3088	T7335	T7336	amod	early,neurons
R3089	T7336	T7334	pobj	neurons,in
R309	T1670	T1662	prep	in,involvement
R3090	T7337	T7336	amod	post-mitotic,neurons
R3091	T7338	T7318	advmod	also,promotes
R3092	T7339	T7340	compound	neurite,outgrowth
R3093	T7340	T7318	dobj	outgrowth,promotes
R3094	T7341	T7318	prep	in,promotes
R3095	T7342	T7343	det	a,manner
R3096	T7343	T7341	pobj	manner,in
R3097	T7344	T7345	npadvmod	neurotrophin,independent
R3098	T7345	T7343	amod	independent,manner
R3099	T7346	T7345	punct	-,independent
R310	T1671	T1672	det	the,control
R3100	T7347	T7316	punct	.,suggest
R3101	T7349	T7350	aux	To,begin
R3102	T7350	T7351	advcl	begin,assayed
R3103	T7352	T7353	aux	to,assess
R3104	T7353	T7350	xcomp	assess,begin
R3105	T7354	T7355	prep	at,become
R3106	T7355	T7353	ccomp	become,assess
R3107	T7356	T7357	det	which,step
R3108	T7357	T7354	pcomp	step,at
R3109	T7358	T7357	prep	of,step
R311	T1672	T1670	pobj	control,in
R3110	T7359	T7360	det	the,cascade
R3111	T7360	T7358	pobj	cascade,of
R3112	T7361	T7360	compound	neurotrophin,cascade
R3113	T7362	T7360	compound	signaling,cascade
R3114	T7363	T7364	compound	DRG,neurons
R3115	T7364	T7355	nsubj	neurons,become
R3116	T7365	T7364	prep	from,neurons
R3117	T7366	T7367	nmod	TauEWS,Isl1Cre
R3118	T7367	T7372	nmod	Isl1Cre,embryos
R3119	T7368	T7367	punct	-,Isl1Cre
R312	T1673	T1672	prep	of,control
R3120	T7369	T7367	nmod	Pea3,Isl1Cre
R3121	T7370	T7367	punct	/,Isl1Cre
R3122	T7371	T7367	punct	+,Isl1Cre
R3123	T7372	T7365	pobj	embryos,from
R3124	T7373	T7367	punct	/,Isl1Cre
R3125	T7374	T7367	punct	+,Isl1Cre
R3126	T7375	T7355	acomp	unresponsive,become
R3127	T7376	T7375	prep	to,unresponsive
R3128	T7377	T7378	det	the,addition
R3129	T7378	T7376	pobj	addition,to
R313	T1674	T1675	amod	neuronal,survival
R3130	T7379	T7378	prep	of,addition
R3131	T7380	T7379	pobj	neurotrophins,of
R3132	T7381	T7351	punct	", ",assayed
R3133	T7382	T7351	nsubj	we,assayed
R3134	T7383	T7384	det	the,expression
R3135	T7384	T7351	dobj	expression,assayed
R3136	T7385	T7384	prep	of,expression
R3137	T7386	T7387	compound	neurotrophin,receptors
R3138	T7387	T7385	pobj	receptors,of
R3139	T7388	T7351	prep	in,assayed
R314	T1675	T1673	pobj	survival,of
R3140	T7389	T7390	nmod	TauEWS,Isl1Cre
R3141	T7390	T7395	nmod	Isl1Cre,embryos
R3142	T7391	T7390	punct	-,Isl1Cre
R3143	T7392	T7390	nmod	Pea3,Isl1Cre
R3144	T7393	T7390	punct	/,Isl1Cre
R3145	T7394	T7390	punct	+,Isl1Cre
R3146	T7395	T7388	pobj	embryos,in
R3147	T7396	T7390	punct	/,Isl1Cre
R3148	T7397	T7390	punct	+,Isl1Cre
R3149	T7398	T7399	punct	(,Figure
R315	T1676	T1677	punct	[,4
R3150	T7399	T7351	parataxis	Figure,assayed
R3151	T7400	T7399	nummod	6,Figure
R3152	T7401	T7399	punct	),Figure
R3153	T7402	T7351	punct	.,assayed
R3154	T7404	T7405	mark	Whereas,marks
R3155	T7405	T7417	advcl	marks,showed
R3156	T7406	T7405	nsubj	expression,marks
R3157	T7407	T7406	prep	of,expression
R3158	T7408	T7409	det	the,receptors
R3159	T7409	T7407	pobj	receptors,of
R316	T1677	T1662	parataxis	4,involvement
R3160	T7410	T7409	compound	neurotrophin,receptors
R3161	T7411	T7409	appos	TrkA,receptors
R3162	T7412	T7411	punct	", ",TrkA
R3163	T7413	T7411	conj	TrkB,TrkA
R3164	T7414	T7413	punct	", ",TrkB
R3165	T7415	T7413	cc	and,TrkB
R3166	T7416	T7413	conj	TrkC,TrkB
R3167	T7418	T7405	dobj	afferents,marks
R3168	T7419	T7418	prep	of,afferents
R3169	T7420	T7421	amod	distinct,modalities
R317	T1678	T1677	punct	],4
R3170	T7421	T7419	pobj	modalities,of
R3171	T7422	T7421	amod	sensory,modalities
R3172	T7423	T7405	prep	in,marks
R3173	T7424	T7423	pobj	DRG,in
R3174	T7425	T7424	prep	of,DRG
R3175	T7426	T7427	amod	wild,type
R3176	T7427	T7429	compound	type,embryos
R3177	T7428	T7427	punct	-,type
R3178	T7429	T7425	pobj	embryos,of
R3179	T7430	T7431	punct	(,6A
R318	T1679	T1629	punct	.,studied
R3180	T7431	T7405	parataxis	6A,marks
R3181	T7432	T7431	compound	Figure,6A
R3182	T7433	T7434	punct	–,6C
R3183	T7434	T7431	prep	6C,6A
R3184	T7435	T7431	punct	),6A
R3185	T7436	T7437	punct	[,29
R3186	T7437	T7405	parataxis	29,marks
R3187	T7438	T7437	dep	4,29
R3188	T7439	T7437	punct	",",29
R3189	T7440	T7437	punct	],29
R319	T1681	T1682	amod	Recent,evidence
R3190	T7441	T7417	punct	", ",showed
R3191	T7442	T7443	nmod	TauEWS,Isl1Cre
R3192	T7443	T7448	nmod	Isl1Cre,embryos
R3193	T7444	T7443	punct	-,Isl1Cre
R3194	T7445	T7443	nmod	Pea3,Isl1Cre
R3195	T7446	T7443	punct	/,Isl1Cre
R3196	T7447	T7443	punct	+,Isl1Cre
R3197	T7448	T7417	nsubj	embryos,showed
R3198	T7449	T7443	punct	/,Isl1Cre
R3199	T7450	T7443	punct	+,Isl1Cre
R320	T1682	T1683	nsubj	evidence,begun
R3200	T7451	T7452	amod	complete,absence
R3201	T7452	T7417	dobj	absence,showed
R3202	T7453	T7452	prep	of,absence
R3203	T7454	T7453	pobj	expression,of
R3204	T7455	T7454	prep	of,expression
R3205	T7456	T7455	pobj	TrkA,of
R3206	T7457	T7456	punct	", ",TrkA
R3207	T7458	T7456	conj	TrkB,TrkA
R3208	T7459	T7458	punct	", ",TrkB
R3209	T7460	T7458	cc	and,TrkB
R321	T1684	T1683	aux	has,begun
R3210	T7461	T7458	conj	TrkC,TrkB
R3211	T7462	T7454	prep	in,expression
R3212	T7463	T7464	compound	DRG,neurons
R3213	T7464	T7462	pobj	neurons,in
R3214	T7465	T7417	prep	at,showed
R3215	T7466	T7465	pobj	E16.5,at
R3216	T7467	T7468	punct	(,6G
R3217	T7468	T7417	parataxis	6G,showed
R3218	T7469	T7468	compound	Figure,6G
R3219	T7470	T7471	punct	–,6I
R322	T1685	T1686	aux	to,emerge
R3220	T7471	T7468	prep	6I,6G
R3221	T7472	T7468	punct	),6G
R3222	T7473	T7417	punct	.,showed
R3223	T7475	T7476	det	This,absence
R3224	T7476	T7477	nsubj	absence,provides
R3225	T7478	T7476	prep	of,absence
R3226	T7479	T7480	compound	Trk,expression
R3227	T7480	T7478	pobj	expression,of
R3228	T7481	T7480	compound	receptor,expression
R3229	T7482	T7476	prep	in,absence
R323	T1686	T1683	xcomp	emerge,begun
R3230	T7483	T7482	pobj	DRG,in
R3231	T7484	T7483	prep	of,DRG
R3232	T7485	T7486	nmod	TauEWS,Isl1Cre
R3233	T7486	T7491	nmod	Isl1Cre,embryos
R3234	T7487	T7486	punct	-,Isl1Cre
R3235	T7488	T7486	nmod	Pea3,Isl1Cre
R3236	T7489	T7486	punct	/,Isl1Cre
R3237	T7490	T7486	punct	+,Isl1Cre
R3238	T7491	T7484	pobj	embryos,of
R3239	T7492	T7486	punct	/,Isl1Cre
R324	T1687	T1688	mark	that,involved
R3240	T7493	T7486	punct	+,Isl1Cre
R3241	T7494	T7495	det	a,explanation
R3242	T7495	T7477	dobj	explanation,provides
R3243	T7496	T7495	amod	likely,explanation
R3244	T7497	T7495	prep	for,explanation
R3245	T7498	T7499	det	the,lack
R3246	T7499	T7497	pobj	lack,for
R3247	T7500	T7499	prep	of,lack
R3248	T7501	T7500	pobj	responsiveness,of
R3249	T7502	T7501	prep	of,responsiveness
R325	T1688	T1683	ccomp	involved,begun
R3250	T7503	T7504	det	these,neurons
R3251	T7504	T7502	pobj	neurons,of
R3252	T7505	T7501	prep	to,responsiveness
R3253	T7506	T7507	det	the,addition
R3254	T7507	T7505	pobj	addition,to
R3255	T7508	T7507	prep	of,addition
R3256	T7509	T7510	amod	neurotrophic,factors
R3257	T7510	T7508	pobj	factors,of
R3258	T7511	T7477	punct	.,provides
R3259	T7513	T7514	nsubj	We,assayed
R326	T1689	T1690	npadvmod	target,derived
R3260	T7515	T7514	advmod	next,assayed
R3261	T7516	T7517	mark	whether,had
R3262	T7517	T7514	ccomp	had,assayed
R3263	T7518	T7519	det	the,absence
R3264	T7519	T7517	nsubj	absence,had
R3265	T7520	T7519	amod	complete,absence
R3266	T7521	T7519	prep	of,absence
R3267	T7522	T7523	compound	Trk,expression
R3268	T7523	T7521	pobj	expression,of
R3269	T7524	T7523	compound	receptor,expression
R327	T1690	T1692	amod	derived,factors
R3270	T7525	T7519	prep	in,absence
R3271	T7526	T7527	nmod	TauEWS,Isl1Cre
R3272	T7527	T7532	nmod	Isl1Cre,embryos
R3273	T7528	T7527	punct	-,Isl1Cre
R3274	T7529	T7527	nmod	Pea3,Isl1Cre
R3275	T7530	T7527	punct	/,Isl1Cre
R3276	T7531	T7527	punct	+,Isl1Cre
R3277	T7532	T7525	pobj	embryos,in
R3278	T7533	T7527	punct	/,Isl1Cre
R3279	T7534	T7527	punct	+,Isl1Cre
R328	T1691	T1690	punct	-,derived
R3280	T7535	T7536	det	an,influence
R3281	T7536	T7517	dobj	influence,had
R3282	T7537	T7536	prep	on,influence
R3283	T7538	T7539	advmod	naturally,occurring
R3284	T7539	T7540	amod	occurring,death
R3285	T7540	T7537	pobj	death,on
R3286	T7541	T7540	compound	cell,death
R3287	T7542	T7543	advmod	in,vivo
R3288	T7543	T7540	advmod	vivo,death
R3289	T7544	T7514	advcl	using,assayed
R329	T1692	T1688	nsubjpass	factors,involved
R3290	T7545	T7544	dobj	TUNEL,using
R3291	T7546	T7544	prep	on,using
R3292	T7547	T7548	compound	DRG,sections
R3293	T7548	T7546	pobj	sections,on
R3294	T7549	T7514	punct	.,assayed
R3295	T7551	T7552	advmod	Surprisingly,found
R3296	T7553	T7552	punct	", ",found
R3297	T7554	T7552	nsubj	we,found
R3298	T7555	T7556	mark	that,decreased
R3299	T7556	T7552	ccomp	decreased,found
R330	T1693	T1688	auxpass	are,involved
R3300	T7557	T7556	nsubjpass	apoptosis,decreased
R3301	T7558	T7556	auxpass	was,decreased
R3302	T7559	T7556	prep	by,decreased
R3303	T7560	T7561	advmod	approximately,50
R3304	T7561	T7562	nummod	50,%
R3305	T7562	T7559	pobj	%,by
R3306	T7563	T7564	punct	(,average
R3307	T7564	T7562	parataxis	average,%
R3308	T7565	T7566	nsubj	n,embryos
R3309	T7566	T7564	ccomp	embryos,average
R331	T1694	T1688	advmod	also,involved
R3310	T7567	T7566	punct	=,embryos
R3311	T7568	T7566	nummod	3,embryos
R3312	T7569	T7564	punct	", ",average
R3313	T7570	T7564	prep	of,average
R3314	T7571	T7572	punct	>,50
R3315	T7572	T7573	nummod	50,sections
R3316	T7573	T7570	pobj	sections,of
R3317	T7574	T7564	punct	),average
R3318	T7575	T7556	prep	in,decreased
R3319	T7576	T7575	pobj	DRG,in
R332	T1695	T1688	prep	in,involved
R3320	T7577	T7576	prep	of,DRG
R3321	T7578	T7579	nmod	TauEWS,Isl1Cre
R3322	T7579	T7584	nmod	Isl1Cre,embryos
R3323	T7580	T7579	punct	-,Isl1Cre
R3324	T7581	T7579	nmod	Pea3,Isl1Cre
R3325	T7582	T7579	punct	/,Isl1Cre
R3326	T7583	T7579	punct	+,Isl1Cre
R3327	T7584	T7577	pobj	embryos,of
R3328	T7585	T7579	punct	/,Isl1Cre
R3329	T7586	T7579	punct	+,Isl1Cre
R333	T1696	T1695	pcomp	regulating,in
R3330	T7587	T7556	prep	in,decreased
R3331	T7588	T7587	pobj	comparison,in
R3332	T7589	T7588	prep	to,comparison
R3333	T7590	T7591	amod	wild,type
R3334	T7591	T7589	pobj	type,to
R3335	T7592	T7591	punct	-,type
R3336	T7593	T7594	punct	(,6D
R3337	T7594	T7556	parataxis	6D,decreased
R3338	T7595	T7594	compound	Figure,6D
R3339	T7596	T7594	punct	", ",6D
R334	T1697	T1698	amod	later,aspects
R3340	T7597	T7594	conj	6J,6D
R3341	T7598	T7597	punct	", ",6J
R3342	T7599	T7597	cc	and,6J
R3343	T7600	T7597	conj	6M,6J
R3344	T7601	T7594	punct	),6D
R3345	T7602	T7552	punct	.,found
R3346	T7604	T7605	amod	Quantitative,analysis
R3347	T7605	T7606	nsubj	analysis,revealed
R3348	T7607	T7605	prep	of,analysis
R3349	T7608	T7609	det	the,number
R335	T1698	T1696	dobj	aspects,regulating
R3350	T7609	T7607	pobj	number,of
R3351	T7610	T7609	prep	of,number
R3352	T7611	T7610	pobj	neurons,of
R3353	T7612	T7611	prep	in,neurons
R3354	T7613	T7614	compound	lumbar,DRG
R3355	T7614	T7612	pobj	DRG,in
R3356	T7615	T7614	prep	of,DRG
R3357	T7616	T7617	nmod	TauEWS,Isl1Cre
R3358	T7617	T7622	nmod	Isl1Cre,embryos
R3359	T7618	T7617	punct	-,Isl1Cre
R336	T1699	T1698	prep	of,aspects
R3360	T7619	T7617	nmod	Pea3,Isl1Cre
R3361	T7620	T7617	punct	/,Isl1Cre
R3362	T7621	T7617	punct	+,Isl1Cre
R3363	T7622	T7615	pobj	embryos,of
R3364	T7623	T7617	punct	/,Isl1Cre
R3365	T7624	T7617	punct	+,Isl1Cre
R3366	T7625	T7626	det	a,increase
R3367	T7626	T7606	dobj	increase,revealed
R3368	T7627	T7626	amod	significant,increase
R3369	T7628	T7626	prep	to,increase
R337	T1700	T1701	amod	neuronal,differentiation
R3370	T7629	T7630	advmod	approximately,170
R3371	T7630	T7631	nummod	170,%
R3372	T7631	T7628	pobj	%,to
R3373	T7632	T7631	prep	of,%
R3374	T7633	T7634	amod	wild,type
R3375	T7634	T7636	compound	type,levels
R3376	T7635	T7634	punct	-,type
R3377	T7636	T7632	pobj	levels,of
R3378	T7637	T7638	punct	(,6E
R3379	T7638	T7606	parataxis	6E,revealed
R338	T1701	T1699	pobj	differentiation,of
R3380	T7639	T7638	compound	Figure,6E
R3381	T7640	T7638	punct	", ",6E
R3382	T7641	T7638	conj	6K,6E
R3383	T7642	T7641	punct	", ",6K
R3384	T7643	T7641	cc	and,6K
R3385	T7644	T7641	conj	6N,6K
R3386	T7645	T7638	punct	),6E
R3387	T7646	T7606	punct	.,revealed
R3388	T7648	T7649	advmod	Moreover,ruled
R3389	T7650	T7649	punct	", ",ruled
R339	T1702	T1703	punct	[,7
R3390	T7651	T7652	compound	BrdU,experiments
R3391	T7652	T7649	nsubj	experiments,ruled
R3392	T7653	T7654	compound	pulse,chase
R3393	T7654	T7652	compound	chase,experiments
R3394	T7655	T7654	punct	-,chase
R3395	T7656	T7649	prt	out,ruled
R3396	T7657	T7658	det	the,possibility
R3397	T7658	T7649	dobj	possibility,ruled
R3398	T7659	T7660	mark	that,reenter
R3399	T7660	T7658	acl	reenter,possibility
R340	T1703	T1683	parataxis	7,begun
R3400	T7661	T7662	compound	DRG,neurons
R3401	T7662	T7660	nsubj	neurons,reenter
R3402	T7663	T7662	prep	in,neurons
R3403	T7664	T7665	nmod	TauEWS,Isl1Cre
R3404	T7665	T7670	nmod	Isl1Cre,embryos
R3405	T7666	T7665	punct	-,Isl1Cre
R3406	T7667	T7665	nmod	Pea3,Isl1Cre
R3407	T7668	T7665	punct	/,Isl1Cre
R3408	T7669	T7665	punct	+,Isl1Cre
R3409	T7670	T7663	pobj	embryos,in
R341	T1704	T1703	nummod	5,7
R3410	T7671	T7665	punct	/,Isl1Cre
R3411	T7672	T7665	punct	+,Isl1Cre
R3412	T7673	T7674	det	the,cycle
R3413	T7674	T7660	dobj	cycle,reenter
R3414	T7675	T7674	compound	cell,cycle
R3415	T7676	T7677	punct	(,6F
R3416	T7677	T7660	parataxis	6F,reenter
R3417	T7678	T7679	det	no,cells
R3418	T7679	T7677	dep	cells,6F
R3419	T7680	T7681	nmod	BrdU,LacZ
R342	T1705	T1703	punct	",",7
R3420	T7681	T7679	nmod	LacZ,cells
R3421	T7682	T7681	punct	+,LacZ
R3422	T7683	T7681	punct	/,LacZ
R3423	T7684	T7681	punct	+,LacZ
R3424	T7685	T7677	punct	", ",6F
R3425	T7686	T7687	nsubj	n,embryos
R3426	T7687	T7677	ccomp	embryos,6F
R3427	T7688	T7687	punct	=,embryos
R3428	T7689	T7687	nummod	3,embryos
R3429	T7690	T7677	punct	", ",6F
R343	T1706	T1703	nummod	6,7
R3430	T7691	T7677	dep	analysis,6F
R3431	T7692	T7691	prep	of,analysis
R3432	T7693	T7694	punct	>,50
R3433	T7694	T7695	nummod	50,sections
R3434	T7695	T7692	pobj	sections,of
R3435	T7751	T7748	conj	support,receptors
R3436	T7696	T7695	advmod	each,sections
R3437	T7752	T7705	punct	.,suggest
R3438	T7697	T7677	punct	;,6F
R3439	T7754	T7755	nsubj	We,analyzed
R344	T1707	T1703	punct	",",7
R3440	T7698	T7677	compound	Figure,6F
R3441	T7756	T7755	advmod	next,analyzed
R3442	T7757	T7758	mark	whether,detected
R3443	T7699	T7677	cc	and,6F
R3444	T7758	T7755	ccomp	detected,analyzed
R3445	T7759	T7758	nsubjpass	changes,detected
R3446	T7760	T7759	prep	in,changes
R3447	T7700	T7677	conj	6L,6F
R3448	T7761	T7762	det	the,expression
R3449	T7762	T7760	pobj	expression,in
R345	T1708	T1703	punct	],7
R3450	T7763	T7762	prep	of,expression
R3451	T7701	T7677	punct	),6F
R3452	T7764	T7763	pobj	proteins,of
R3453	T7765	T7764	acl	known,proteins
R3454	T7702	T7649	punct	.,ruled
R3455	T7766	T7767	aux	to,involved
R3456	T7767	T7765	xcomp	involved,known
R3457	T7768	T7767	auxpass	be,involved
R3458	T7704	T7705	advmod	Together,suggest
R3459	T7769	T7767	prep	in,involved
R346	T1709	T1683	punct	.,begun
R3460	T7770	T7771	det	the,regulation
R3461	T7771	T7769	pobj	regulation,in
R3462	T7772	T7771	prep	of,regulation
R3463	T7773	T7774	amod	neuronal,survival
R3464	T7706	T7704	prep	with,Together
R3465	T7774	T7772	pobj	survival,of
R3466	T7775	T7774	cc	or,survival
R3467	T7776	T7777	compound	cell,death
R3468	T7707	T7708	det	the,experiments
R3469	T7777	T7774	conj	death,survival
R347	T1711	T1712	prep	In,addressed
R3470	T7778	T7758	aux	could,detected
R3471	T7779	T7758	auxpass	be,detected
R3472	T7708	T7706	pobj	experiments,with
R3473	T7780	T7758	prep	in,detected
R3474	T7781	T7780	pobj	DRG,in
R3475	T7782	T7781	prep	of,DRG
R3476	T7709	T7710	advmod	in,vitro
R3477	T7783	T7784	nmod	TauEWS,Isl1Cre
R3478	T7784	T7789	nmod	Isl1Cre,embryos
R3479	T7710	T7708	advmod	vitro,experiments
R348	T1713	T1711	amod	particular,In
R3480	T7785	T7784	punct	-,Isl1Cre
R3481	T7786	T7784	nmod	Pea3,Isl1Cre
R3482	T7787	T7784	punct	/,Isl1Cre
R3483	T7711	T7708	compound	culture,experiments
R3484	T7788	T7784	punct	+,Isl1Cre
R3485	T7789	T7782	pobj	embryos,of
R3486	T7790	T7784	punct	/,Isl1Cre
R3487	T7791	T7784	punct	+,Isl1Cre
R3488	T7792	T7755	punct	.,analyzed
R3489	T7712	T7705	punct	", ",suggest
R349	T1714	T1712	punct	", ",addressed
R3490	T7794	T7795	nsubj	We,found
R3491	T7796	T7797	det	no,changes
R3492	T7713	T7714	det	these,findings
R3493	T7797	T7795	dobj	changes,found
R3494	T7798	T7797	amod	significant,changes
R3495	T7799	T7797	amod	quantitative,changes
R3496	T7714	T7705	nsubj	findings,suggest
R3497	T7800	T7797	prep	in,changes
R3498	T7801	T7802	det	the,level
R3499	T7715	T7716	mark	that,remain
R350	T1715	T1716	amod	genetic,experiments
R3500	T7716	T7705	ccomp	remain,suggest
R3501	T7802	T7800	pobj	level,in
R3502	T7803	T7802	prep	of,level
R3503	T7717	T7718	compound	DRG,neurons
R3504	T7804	T7805	compound	Akt,Akt
R3505	T7805	T7803	pobj	Akt,of
R3506	T7806	T7805	punct	/,Akt
R3507	T7718	T7716	nsubj	neurons,remain
R3508	T7807	T7805	compound	p,Akt
R3509	T7808	T7805	punct	-,Akt
R351	T1716	T1712	nsubj	experiments,addressed
R3510	T7809	T7805	cc	or,Akt
R3511	T7719	T7718	prep	from,neurons
R3512	T7810	T7811	compound	CREB,CREB
R3513	T7811	T7805	conj	CREB,Akt
R3514	T7720	T7721	nmod	TauEWS,Isl1Cre
R3515	T7812	T7811	punct	/,CREB
R3516	T7813	T7811	compound	p,CREB
R3517	T7721	T7726	nmod	Isl1Cre,embryos
R3518	T7814	T7811	punct	-,CREB
R3519	T7815	T7802	prep	in,level
R352	T1717	T1712	aux	have,addressed
R3520	T7816	T7815	pobj	DRG,in
R3521	T7722	T7721	punct	-,Isl1Cre
R3522	T7817	T7818	punct	(,6O
R3523	T7818	T7816	parataxis	6O,DRG
R3524	T7723	T7721	nmod	Pea3,Isl1Cre
R3525	T7819	T7818	compound	Figure,6O
R3526	T7820	T7818	cc	and,6O
R3527	T7821	T7818	conj	6P,6O
R3528	T7724	T7721	punct	/,Isl1Cre
R3529	T7822	T7818	punct	),6O
R353	T1718	T1719	det	the,role
R3530	T7823	T7824	dep	both,shown
R3531	T7725	T7721	punct	+,Isl1Cre
R3532	T7824	T7802	relcl	shown,level
R3533	T7825	T7823	prep	of,both
R3534	T7826	T7825	pobj	which,of
R3535	T7726	T7719	pobj	embryos,from
R3536	T7827	T7824	aux	have,shown
R3537	T7828	T7824	auxpass	been,shown
R3538	T7829	T7830	aux	to,be
R3539	T7830	T7824	xcomp	be,shown
R354	T1719	T1712	dobj	role,addressed
R3540	T7831	T7832	amod	key,regulators
R3541	T7832	T7830	attr	regulators,be
R3542	T7727	T7721	punct	/,Isl1Cre
R3543	T7833	T7832	prep	of,regulators
R3544	T7834	T7835	amod	neuronal,survival
R3545	T7835	T7833	pobj	survival,of
R3546	T7728	T7721	punct	+,Isl1Cre
R3547	T7836	T7837	punct	[,29
R3548	T7837	T7824	parataxis	29,shown
R3549	T7838	T7837	punct	],29
R355	T1720	T1721	npadvmod	survival,independent
R3550	T7729	T7716	oprd	post-mitotic,remain
R3551	T7839	T7795	punct	.,found
R3552	T7730	T7716	cc	but,remain
R3553	T7841	T7842	advmod	Moreover,reduced
R3554	T7843	T7842	punct	", ",reduced
R3555	T7731	T7716	conj	fail,remain
R3556	T7732	T7733	aux	to,become
R3557	T7844	T7845	det	the,level
R3558	T7845	T7842	nsubjpass	level,reduced
R3559	T7733	T7731	xcomp	become,fail
R356	T1721	T1719	amod	independent,role
R3560	T7846	T7845	prep	of,level
R3561	T7847	T7848	det	the,Bax
R3562	T7734	T7733	acomp	sensitive,become
R3563	T7848	T7846	pobj	Bax,of
R3564	T7849	T7848	amod	proapoptotic,Bax
R3565	T7850	T7848	compound	Bcl2,Bax
R3566	T7735	T7734	prep	to,sensitive
R3567	T7851	T7848	compound	family,Bax
R3568	T7852	T7848	compound	member,Bax
R3569	T7853	T7842	auxpass	was,reduced
R357	T1722	T1721	punct	-,independent
R3570	T7736	T7737	advmod	naturally,occurring
R3571	T7854	T7842	neg	not,reduced
R3572	T7855	T7842	advmod	significantly,reduced
R3573	T7737	T7738	amod	occurring,death
R3574	T7738	T7735	pobj	death,to
R3575	T7739	T7738	compound	cell,death
R3576	T7740	T7731	punct	", ",fail
R3577	T7856	T7857	punct	(,6O
R3578	T7857	T7842	parataxis	6O,reduced
R3579	T7741	T7731	cc	and,fail
R358	T1723	T1719	prep	of,role
R3580	T7858	T7857	compound	Figure,6O
R3581	T7859	T7857	cc	and,6O
R3582	T7860	T7857	conj	6P,6O
R3583	T7742	T7731	conj	survive,fail
R3584	T7861	T7857	punct	),6O
R3585	T7862	T7842	punct	.,reduced
R3586	T7743	T7742	prep	in,survive
R3587	T7864	T7865	prep	In,increased
R3588	T7744	T7745	det	the,absence
R3589	T7866	T7864	pobj	contrast,In
R359	T1724	T1725	amod	neurotrophic,factors
R3590	T7867	T7865	punct	", ",increased
R3591	T7868	T7869	det	the,level
R3592	T7869	T7865	nsubjpass	level,increased
R3593	T7870	T7869	compound	expression,level
R3594	T7745	T7743	pobj	absence,in
R3595	T7871	T7869	prep	of,level
R3596	T7872	T7873	det	the,members
R3597	T7873	T7871	pobj	members,of
R3598	T7746	T7745	prep	of,absence
R3599	T7874	T7873	amod	anti-apoptotic,members
R360	T1725	T1723	pobj	factors,of
R3600	T7875	T7873	compound	Bcl2,members
R3601	T7876	T7873	compound	family,members
R3602	T7747	T7748	compound	Trk,receptors
R3603	T7877	T7878	compound	Bcl,xl
R3604	T7878	T7873	appos	xl,members
R3605	T7879	T7878	punct	-,xl
R3606	T7748	T7746	pobj	receptors,of
R3607	T7880	T7878	cc	and,xl
R3608	T7881	T7878	conj	Bcl2,xl
R3609	T7882	T7865	auxpass	was,increased
R361	T1726	T1719	prep	during,role
R3610	T7749	T7748	cc	and,receptors
R3611	T7883	T7865	advmod	significantly,increased
R3612	T7884	T7885	advmod	when,compared
R3613	T7885	T7865	advcl	compared,increased
R3614	T7750	T7751	amod	neurotrophic,support
R3615	T7886	T7885	prep	to,compared
R3616	T7887	T7888	amod	wild,type
R3617	T7888	T7890	compound	type,levels
R3618	T7889	T7888	punct	-,type
R3619	T7890	T7886	pobj	levels,to
R362	T1727	T1726	pobj	development,during
R3620	T7891	T7892	punct	(,6O
R3621	T7892	T7890	parataxis	6O,levels
R3622	T7893	T7894	dep	Bcl2,%
R3623	T7894	T7892	dep	%,6O
R3624	T7895	T7894	punct	", ",%
R3625	T7896	T7894	nummod	157,%
R3626	T7897	T7892	punct	;,6O
R3627	T7898	T7899	compound	Bcl,xl
R3628	T7899	T7901	dep	xl,%
R3629	T7900	T7899	punct	-,xl
R363	T1728	T1712	prep	by,addressed
R3630	T7901	T7892	dep	%,6O
R3631	T7902	T7901	punct	", ",%
R3632	T7903	T7901	nummod	259,%
R3633	T7904	T7892	punct	;,6O
R3634	T7905	T7892	dep	average,6O
R3635	T7906	T7905	prep	of,average
R3636	T7907	T7908	nsubj	n,experiments
R3637	T7908	T7906	pcomp	experiments,of
R3638	T7909	T7908	punct	=,experiments
R3639	T7910	T7908	nummod	3,experiments
R364	T1729	T1728	pcomp	exploiting,by
R3640	T7911	T7908	amod	independent,experiments
R3641	T7912	T7892	punct	;,6O
R3642	T7913	T7892	compound	Figure,6O
R3643	T7914	T7892	cc	and,6O
R3644	T7915	T7892	conj	6P,6O
R3645	T7916	T7892	punct	),6O
R3646	T7917	T7865	punct	", ",increased
R3647	T7918	T7865	advcl	providing,increased
R3648	T7919	T7920	det	a,explanation
R3649	T7920	T7918	dobj	explanation,providing
R365	T1730	T1729	dobj	strains,exploiting
R3650	T7921	T7920	amod	potential,explanation
R3651	T7922	T7920	amod	molecular,explanation
R3652	T7923	T7920	prep	for,explanation
R3653	T7924	T7925	det	the,survival
R3654	T7925	T7923	pobj	survival,for
R3655	T7926	T7925	amod	enhanced,survival
R3656	T7927	T7925	amod	neuronal,survival
R3657	T7928	T7925	prep	of,survival
R3658	T7929	T7930	compound	DRG,neurons
R3659	T7930	T7928	pobj	neurons,of
R366	T1731	T1730	prep	of,strains
R3660	T7931	T7930	prep	of,neurons
R3661	T7932	T7933	nmod	TauEWS,Isl1Cre
R3662	T7933	T7938	nmod	Isl1Cre,embryos
R3663	T7934	T7933	punct	-,Isl1Cre
R3664	T7935	T7933	nmod	Pea3,Isl1Cre
R3665	T7936	T7933	punct	/,Isl1Cre
R3666	T7937	T7933	punct	+,Isl1Cre
R3667	T7938	T7931	pobj	embryos,of
R3668	T7939	T7933	punct	/,Isl1Cre
R3669	T7940	T7933	punct	+,Isl1Cre
R367	T1732	T1731	pobj	mice,of
R3670	T7941	T7925	prep	in,survival
R3671	T7942	T7943	det	the,absence
R3672	T7943	T7941	pobj	absence,in
R3673	T7944	T7943	prep	of,absence
R3674	T7945	T7946	compound	Trk,receptor
R3675	T7946	T7947	compound	receptor,expression
R3676	T7947	T7944	pobj	expression,of
R3677	T7948	T7949	punct	[,30
R3678	T7949	T7918	parataxis	30,providing
R3679	T7950	T7949	punct	],30
R368	T1733	T1730	amod	defective,strains
R3680	T7951	T7865	punct	.,increased
R3683	T8705	T8706	advmod	Only,Signaling
R3684	T8706	T8712	nsubj	Signaling,Interferes
R3685	T8707	T8706	amod	Precocious,Signaling
R3686	T8708	T8707	cc	but,Precocious
R3687	T8709	T8708	neg	Not,but
R3688	T8710	T8707	conj	Isochronic,Precocious
R3689	T8711	T8706	compound	ETS,Signaling
R369	T1734	T1735	preconj	both,in
R3690	T8713	T8706	prep	in,Signaling
R3691	T8714	T8715	compound	DRG,Neurons
R3692	T8715	T8713	pobj	Neurons,in
R3693	T8716	T8712	prep	with,Interferes
R3694	T8717	T8718	amod	Neuronal,Fate
R3695	T8718	T8719	compound	Fate,Acquisition
R3696	T8719	T8716	pobj	Acquisition,with
R3697	T8721	T8722	det	The,differences
R3698	T8722	T8724	nsubj	differences,prompted
R3699	T8723	T8722	amod	observed,differences
R370	T1735	T1733	prep	in,defective
R3700	T8725	T8722	prep	in,differences
R3701	T8726	T8727	amod	neuronal,survival
R3702	T8727	T8725	pobj	survival,in
R3703	T8728	T8727	cc	and,survival
R3704	T8729	T8730	compound	neurite,outgrowth
R3705	T8730	T8727	conj	outgrowth,survival
R3706	T8731	T8722	prep	between,differences
R3707	T8732	T8733	amod	precocious,expression
R3708	T8733	T8731	pobj	expression,between
R3709	T8734	T8732	cc	and,precocious
R371	T1736	T1737	compound	neurotrophin,signaling
R3710	T8735	T8732	conj	isochronic,precocious
R3711	T8736	T8733	prep	of,expression
R3712	T8737	T8738	compound	EWS,Pea3
R3713	T8738	T8736	pobj	Pea3,of
R3714	T8739	T8738	punct	-,Pea3
R3715	T8740	T8724	dobj	us,prompted
R3716	T8741	T8742	aux	to,perform
R3717	T8742	T8724	xcomp	perform,prompted
R3718	T8743	T8744	det	a,analysis
R3719	T8744	T8742	dobj	analysis,perform
R372	T1737	T1735	pobj	signaling,in
R3720	T8745	T8744	amod	direct,analysis
R3721	T8746	T8744	amod	comparative,analysis
R3722	T8747	T8744	prep	of,analysis
R3723	T8748	T8749	compound	gene,expression
R3724	T8749	T8747	pobj	expression,of
R3725	T8750	T8749	prep	between,expression
R3726	T8751	T8750	pobj	mice,between
R3727	T8752	T8751	prep	with,mice
R3728	T8753	T8754	amod	precocious,expression
R3729	T8754	T8752	pobj	expression,with
R373	T1738	T1735	cc	and,in
R3730	T8755	T8756	compound	EWS,Pea3
R3731	T8756	T8754	compound	Pea3,expression
R3732	T8757	T8756	punct	-,Pea3
R3733	T8758	T8751	cc	and,mice
R3734	T8759	T8751	conj	mice,mice
R3735	T8760	T8761	prep	in,initiated
R3736	T8761	T8759	relcl	initiated,mice
R3737	T8762	T8760	pobj	which,in
R3738	T8763	T8764	det	the,expression
R3739	T8764	T8761	nsubjpass	expression,initiated
R374	T1739	T1735	conj	in,in
R3740	T8765	T8764	prep	of,expression
R3741	T8766	T8767	compound	EWS,Pea3
R3742	T8767	T8765	pobj	Pea3,of
R3743	T8768	T8767	punct	-,Pea3
R3744	T8769	T8761	auxpass	is,initiated
R3745	T8770	T8761	prep	in,initiated
R3746	T8771	T8772	nmod	DRG,neurons
R3747	T8772	T8770	pobj	neurons,in
R3748	T8773	T8772	amod	sensory,neurons
R3749	T8774	T8761	prep	from,initiated
R375	T1740	T1741	det	the,function
R3750	T8775	T8776	det	the,time
R3751	T8776	T8774	pobj	time,from
R3752	T8777	T8776	prep	of,time
R3753	T8778	T8779	amod	normal,onset
R3754	T8779	T8777	pobj	onset,of
R3755	T8780	T8779	prep	of,onset
R3756	T8781	T8782	compound	Er81,expression
R3757	T8782	T8780	pobj	expression,of
R3758	T8783	T8724	punct	.,prompted
R3759	T8785	T8786	advmod	Moreover,performed
R376	T1741	T1739	pobj	function,in
R3760	T8787	T8786	punct	", ",performed
R3761	T8788	T8789	aux	to,rule
R3762	T8789	T8786	advcl	rule,performed
R3763	T8790	T8789	prt	out,rule
R3764	T8791	T8792	det	the,possibility
R3765	T8792	T8789	dobj	possibility,rule
R3766	T8793	T8794	mark	that,be
R3767	T8794	T8792	acl	be,possibility
R3768	T8795	T8796	det	a,effect
R3769	T8796	T8794	nsubj	effect,be
R377	T1742	T1741	prep	of,function
R3770	T8797	T8796	amod	differential,effect
R3771	T8798	T8794	aux	may,be
R3772	T8799	T8794	prep	due,be
R3773	T8800	T8799	pcomp	to,due
R3774	T8801	T8802	det	the,strategies
R3775	T8802	T8799	pobj	strategies,due
R3776	T8803	T8802	amod	different,strategies
R3777	T8804	T8802	amod	genetic,strategies
R3778	T8805	T8806	prep	by,achieved
R3779	T8806	T8802	relcl	achieved,strategies
R378	T1743	T1744	det	the,Bax
R3780	T8807	T8805	pobj	which,by
R3781	T8808	T8806	nsubjpass	expression,achieved
R3782	T8809	T8808	prep	of,expression
R3783	T8810	T8811	compound	EWS,Pea3
R3784	T8811	T8809	pobj	Pea3,of
R3785	T8812	T8811	punct	-,Pea3
R3786	T8813	T8808	prep	in,expression
R3787	T8814	T8815	amod	proprioceptive,afferents
R3788	T8815	T8813	pobj	afferents,in
R3789	T8816	T8806	auxpass	is,achieved
R379	T1744	T1742	pobj	Bax,of
R3790	T8817	T8786	punct	", ",performed
R3791	T8818	T8786	nsubj	we,performed
R3792	T8819	T8820	det	this,analysis
R3793	T8820	T8786	dobj	analysis,performed
R3794	T8821	T8822	preconj	both,in
R3795	T8822	T8786	prep	in,performed
R3796	T8823	T8824	nmod	Er81EWS,Pea3
R3797	T8824	T8826	nmod	Pea3,embryos
R3798	T8825	T8824	punct	-,Pea3
R3799	T8826	T8822	pobj	embryos,in
R380	T1745	T1744	amod	proapoptotic,Bax
R3800	T8827	T8824	punct	/,Pea3
R3801	T8828	T8824	punct	−,Pea3
R3802	T8829	T8824	cc	and,Pea3
R3803	T8830	T8831	nmod	TauEWS,PVCre
R3804	T8831	T8824	conj	PVCre,Pea3
R3805	T8832	T8831	punct	-,PVCre
R3806	T8833	T8831	nmod	Pea3,PVCre
R3807	T8834	T8831	punct	/,PVCre
R3808	T8835	T8831	punct	+,PVCre
R3809	T8836	T8831	punct	/,PVCre
R381	T1746	T1744	compound	gene,Bax
R3810	T8837	T8831	punct	+,PVCre
R3811	T8838	T8786	punct	.,performed
R3812	T8840	T8841	nsubj	We,analyzed
R3813	T8842	T8841	advmod	first,analyzed
R3814	T8843	T8841	dobj	expression,analyzed
R3815	T8844	T8843	prep	of,expression
R3816	T8845	T8844	pobj	TrkC,of
R3817	T8846	T8845	punct	", ",TrkC
R3818	T8847	T8848	det	a,gene
R3819	T8848	T8845	appos	gene,TrkC
R382	T1747	T1748	punct	[,9
R3820	T8849	T8848	acl	downregulated,gene
R3821	T8850	T8849	prep	in,downregulated
R3822	T8851	T8852	compound	DRG,neurons
R3823	T8852	T8850	pobj	neurons,in
R3824	T8853	T8852	prep	of,neurons
R3825	T8854	T8855	nmod	TauEWS,Isl1Cre
R3826	T8855	T8860	nmod	Isl1Cre,embryos
R3827	T8856	T8855	punct	-,Isl1Cre
R3828	T8857	T8855	nmod	Pea3,Isl1Cre
R3829	T8858	T8855	punct	/,Isl1Cre
R383	T1748	T1729	parataxis	9,exploiting
R3830	T8859	T8855	punct	+,Isl1Cre
R3831	T8860	T8853	pobj	embryos,of
R3832	T8861	T8855	punct	/,Isl1Cre
R3833	T8862	T8855	punct	+,Isl1Cre
R3834	T8863	T8864	punct	(,7A
R3835	T8864	T8841	parataxis	7A,analyzed
R3836	T8865	T8864	compound	Figure,7A
R3837	T8866	T8864	cc	and,7A
R3838	T8867	T8864	conj	7B,7A
R3839	T8868	T8864	punct	),7A
R384	T1749	T1748	nummod	8,9
R3840	T8869	T8841	punct	.,analyzed
R3841	T8871	T8872	det	The,level
R3842	T8872	T8873	nsubj	level,was
R3843	T8874	T8872	prep	of,level
R3844	T8875	T8874	pobj	expression,of
R3845	T8876	T8875	prep	of,expression
R3846	T8877	T8876	pobj	TrkC,of
R3847	T8878	T8873	acomp	indistinguishable,was
R3848	T8879	T8878	prep	from,indistinguishable
R3849	T8880	T8881	amod	wild,type
R385	T1750	T1748	punct	",",9
R3850	T8881	T8879	pobj	type,from
R3851	T8882	T8881	punct	-,type
R3852	T8883	T8873	prep	in,was
R3853	T8884	T8885	compound	DRG,neurons
R3854	T8885	T8883	pobj	neurons,in
R3855	T8886	T8885	prep	of,neurons
R3856	T8887	T8888	nmod	Er81EWS,Pea3
R3857	T8888	T8890	nmod	Pea3,embryos
R3858	T8889	T8888	punct	-,Pea3
R3859	T8890	T8886	pobj	embryos,of
R386	T1751	T1748	punct	],9
R3860	T8891	T8888	punct	/,Pea3
R3861	T8892	T8888	punct	−,Pea3
R3862	T8893	T8888	cc	and,Pea3
R3863	T8894	T8895	nmod	TauEWS,PVCre
R3864	T8895	T8888	conj	PVCre,Pea3
R3865	T8896	T8895	punct	-,PVCre
R3866	T8897	T8895	nmod	Pea3,PVCre
R3867	T8898	T8895	punct	/,PVCre
R3868	T8899	T8895	punct	+,PVCre
R3869	T8900	T8895	punct	/,PVCre
R387	T1752	T1712	punct	.,addressed
R3870	T8901	T8895	punct	+,PVCre
R3871	T8902	T8903	punct	(,7A
R3872	T8903	T8873	parataxis	7A,was
R3873	T8904	T8903	compound	Figure,7A
R3874	T8905	T8903	punct	", ",7A
R3875	T8906	T8903	conj	7C,7A
R3876	T8907	T8906	punct	", ",7C
R3877	T8908	T8906	cc	and,7C
R3878	T8909	T8906	conj	7D,7C
R3879	T8910	T8903	punct	),7A
R388	T1754	T1755	det	These,studies
R3880	T8911	T8873	punct	.,was
R3881	T8913	T8914	advmod	Moreover,expressed
R3882	T8915	T8914	punct	", ",expressed
R3883	T8916	T8914	nsubjpass	PV,expressed
R3884	T8917	T8914	auxpass	was,expressed
R3885	T8918	T8914	neg	not,expressed
R3886	T8919	T8914	prep	in,expressed
R3887	T8920	T8921	compound	DRG,neurons
R3888	T8921	T8919	pobj	neurons,in
R3889	T8922	T8921	prep	of,neurons
R389	T1755	T1756	nsubj	studies,revealed
R3890	T8923	T8924	nmod	TauEWS,Isl1Cre
R3891	T8924	T8929	nmod	Isl1Cre,embryos
R3892	T8925	T8924	punct	-,Isl1Cre
R3893	T8926	T8924	nmod	Pea3,Isl1Cre
R3894	T8927	T8924	punct	/,Isl1Cre
R3895	T8928	T8924	punct	+,Isl1Cre
R3896	T8929	T8922	pobj	embryos,of
R3897	T8930	T8924	punct	/,Isl1Cre
R3898	T8931	T8924	punct	+,Isl1Cre
R3899	T8932	T8933	punct	(,S5
R390	T1863	T1864	amod	peptidergic,neurons
R3900	T8933	T8914	parataxis	S5,expressed
R3901	T8934	T8933	compound	Figure,S5
R3902	T8935	T8933	punct	),S5
R3903	T8936	T8914	cc	but,expressed
R3904	T8937	T8938	auxpass	was,expressed
R3905	T8938	T8914	conj	expressed,expressed
R3906	T8939	T8938	agent	by,expressed
R3907	T8940	T8941	amod	proprioceptive,afferents
R3908	T8941	T8939	pobj	afferents,by
R3909	T8942	T8938	prep	in,expressed
R391	T1864	T1862	pobj	neurons,of
R3910	T8943	T8944	preconj	both,type
R3911	T8944	T8947	nmod	type,embryos
R3912	T8945	T8944	amod	wild,type
R3913	T8946	T8944	punct	-,type
R3914	T8947	T8942	pobj	embryos,in
R3915	T8948	T8944	cc	and,type
R3916	T8949	T8950	compound	Er81EWS,Pea3
R3917	T8950	T8944	conj	Pea3,type
R3918	T8951	T8950	punct	-,Pea3
R3919	T8952	T8950	punct	/,Pea3
R392	T1865	T1861	acl	expressing,subpopulation
R3920	T8953	T8950	punct	−,Pea3
R3921	T8954	T8955	punct	(,see
R3922	T8955	T8938	parataxis	see,expressed
R3923	T8956	T8957	nmod	Figures,S5
R3924	T8957	T8955	dobj	S5,see
R3925	T8958	T8957	nummod	1,S5
R3926	T8959	T8957	cc	and,S5
R3927	T8960	T8955	punct	),see
R3928	T8961	T8962	punct	[,14
R3929	T8962	T8938	parataxis	14,expressed
R393	T1866	T1865	dobj	Apterous,expressing
R3930	T8963	T8962	punct	],14
R3931	T8964	T8914	punct	.,expressed
R3932	T8966	T8967	nsubj	We,found
R3933	T8968	T8967	advmod	also,found
R3934	T8969	T8970	amod	several,genes
R3935	T8970	T8967	dobj	genes,found
R3936	T8971	T8972	dep	that,upregulated
R3937	T8972	T8970	relcl	upregulated,genes
R3938	T8973	T8972	auxpass	were,upregulated
R3939	T8974	T8972	advmod	ectopically,upregulated
R394	T1867	T1866	cc	and,Apterous
R3940	T8975	T8972	prep	in,upregulated
R3941	T8976	T8977	compound	DRG,neurons
R3942	T8977	T8975	pobj	neurons,in
R3943	T8978	T8977	prep	of,neurons
R3944	T8979	T8980	nmod	TauEWS,Isl1Cre
R3945	T8980	T8985	nmod	Isl1Cre,embryos
R3946	T8981	T8980	punct	-,Isl1Cre
R3947	T8982	T8980	nmod	Pea3,Isl1Cre
R3948	T8983	T8980	punct	/,Isl1Cre
R3949	T8984	T8980	punct	+,Isl1Cre
R395	T1757	T1756	punct	", ",revealed
R3950	T8985	T8978	pobj	embryos,of
R3951	T8986	T8980	punct	/,Isl1Cre
R3952	T8987	T8980	punct	+,Isl1Cre
R3953	T8988	T8989	punct	(,Figure
R3954	T8989	T8972	parataxis	Figure,upregulated
R3955	T8990	T8989	nummod	7,Figure
R3956	T8991	T8989	punct	),Figure
R3957	T8992	T8967	punct	.,found
R3958	T8994	T8995	nsubjpass	Calretinin,induced
R3959	T8996	T8994	cc	and,Calretinin
R396	T1868	T1866	conj	Squeeze,Apterous
R3960	T8997	T8994	conj	Calbindin,Calretinin
R3961	T8998	T8994	punct	", ",Calretinin
R3962	T8999	T9000	nummod	two,proteins
R3963	T9000	T8994	appos	proteins,Calretinin
R3964	T9001	T9000	amod	different,proteins
R3965	T9002	T9003	npadvmod	calcium,binding
R3966	T9003	T9000	amod	binding,proteins
R3967	T9004	T9003	punct	-,binding
R3968	T9005	T9000	acl	expressed,proteins
R3969	T9006	T9005	agent	by,expressed
R397	T1758	T1756	prep	for,revealed
R3970	T9007	T9006	pobj	subpopulations,by
R3971	T9008	T9007	prep	of,subpopulations
R3972	T9009	T9010	compound	DRG,neurons
R3973	T9010	T9008	pobj	neurons,of
R3974	T9011	T9007	prep	in,subpopulations
R3975	T9012	T9013	amod	wild,type
R3976	T9013	T9015	nmod	type,embryos
R3977	T9014	T9013	punct	-,type
R3978	T9015	T9011	pobj	embryos,in
R3979	T9016	T9013	punct	", ",type
R398	T1869	T1870	punct	[,12
R3980	T9017	T9018	compound	Er81EWS,Pea3
R3981	T9018	T9013	conj	Pea3,type
R3982	T9019	T9018	punct	-,Pea3
R3983	T9020	T9018	punct	/,Pea3
R3984	T9021	T9018	punct	−,Pea3
R3985	T9022	T9018	punct	", ",Pea3
R3986	T9023	T9018	cc	and,Pea3
R3987	T9024	T9025	nmod	TauEWS,PVCre
R3988	T9025	T9018	conj	PVCre,Pea3
R3989	T9026	T9025	punct	-,PVCre
R399	T1759	T1758	pobj	example,for
R3990	T9027	T9025	nmod	Pea3,PVCre
R3991	T9028	T9025	punct	/,PVCre
R3992	T9029	T9025	punct	+,PVCre
R3993	T9030	T9025	punct	/,PVCre
R3994	T9031	T9025	punct	+,PVCre
R3995	T9032	T9033	punct	(,7E
R3996	T9033	T8994	parataxis	7E,Calretinin
R3997	T9034	T9033	compound	Figure,7E
R3998	T9035	T9033	punct	", ",7E
R3999	T9036	T9033	conj	7G,7E
R400	T1760	T1756	punct	", ",revealed
R4000	T9037	T9036	punct	", ",7G
R4001	T9038	T9036	cc	and,7G
R4002	T9039	T9036	conj	7H,7G
R4003	T9040	T9033	punct	;,7E
R4004	T9041	T9042	nsubj	data,shown
R4005	T9042	T9033	ccomp	shown,7E
R4006	T9043	T9042	neg	not,shown
R4007	T9044	T9033	punct	),7E
R4008	T9045	T9046	punct	[,32
R4009	T9046	T8994	parataxis	32,Calretinin
R401	T1870	T1846	parataxis	12,control
R4010	T9047	T9046	nummod	31,32
R4011	T9048	T9046	punct	",",32
R4012	T9049	T9046	punct	],32
R4013	T9050	T8995	punct	", ",induced
R4014	T9051	T8995	auxpass	were,induced
R4015	T9052	T8995	prep	in,induced
R4016	T9053	T9054	amod	more,95
R4017	T9054	T9056	nummod	95,%
R4018	T9055	T9054	quantmod	than,95
R4019	T9056	T9052	pobj	%,in
R402	T1761	T1756	aux	have,revealed
R4020	T9057	T9056	prep	of,%
R4021	T9058	T9059	det	all,neurons
R4022	T9059	T9057	pobj	neurons,of
R4023	T9060	T9059	compound	DRG,neurons
R4024	T9061	T9059	prep	of,neurons
R4025	T9062	T9063	nmod	TauEWS,Isl1Cre
R4026	T9063	T9068	nmod	Isl1Cre,embryos
R4027	T9064	T9063	punct	-,Isl1Cre
R4028	T9065	T9063	nmod	Pea3,Isl1Cre
R4029	T9066	T9063	punct	/,Isl1Cre
R403	T1762	T1763	mark	that,controls
R4030	T9067	T9063	punct	+,Isl1Cre
R4031	T9068	T9061	pobj	embryos,of
R4032	T9069	T9063	punct	/,Isl1Cre
R4033	T9070	T9063	punct	+,Isl1Cre
R4034	T9071	T9072	punct	(,7F
R4035	T9072	T8995	parataxis	7F,induced
R4036	T9073	T9072	compound	Figures,7F
R4037	T9074	T9072	cc	and,7F
R4038	T9075	T9072	conj	S5,7F
R4039	T9076	T9072	punct	),7F
R404	T1871	T1870	nummod	11,12
R4040	T9077	T8995	punct	.,induced
R4041	T9079	T9080	det	These,findings
R4042	T9080	T9081	nsubj	findings,suggest
R4043	T9082	T9083	mark	that,fail
R4044	T9083	T9081	ccomp	fail,suggest
R4045	T9084	T9085	compound	DRG,neurons
R4046	T9085	T9083	nsubj	neurons,fail
R4047	T9086	T9085	prep	in,neurons
R4048	T9087	T9088	nmod	TauEWS,Isl1Cre
R4049	T9088	T9093	nmod	Isl1Cre,embryos
R405	T1763	T1756	ccomp	controls,revealed
R4050	T9089	T9088	punct	-,Isl1Cre
R4051	T9090	T9088	nmod	Pea3,Isl1Cre
R4052	T9091	T9088	punct	/,Isl1Cre
R4053	T9092	T9088	punct	+,Isl1Cre
R4054	T9093	T9086	pobj	embryos,in
R4055	T9094	T9088	punct	/,Isl1Cre
R4056	T9095	T9088	punct	+,Isl1Cre
R4057	T9096	T9097	aux	to,differentiate
R4058	T9097	T9083	xcomp	differentiate,fail
R4059	T9098	T9097	prep	to,differentiate
R406	T1764	T1765	compound	neurotrophin,signaling
R4060	T9099	T9100	det	a,fate
R4061	T9100	T9098	pobj	fate,to
R4062	T9101	T9100	amod	normal,fate
R4063	T9102	T9083	cc	and,fail
R4064	T9103	T9104	advmod	instead,acquire
R4065	T9104	T9083	conj	acquire,fail
R4066	T9105	T9106	det	an,identity
R4067	T9106	T9104	dobj	identity,acquire
R4068	T9107	T9106	amod	aberrant,identity
R4069	T9108	T9106	amod	distinct,identity
R407	T1872	T1870	punct	",",12
R4070	T9109	T9108	prep	from,distinct
R4071	T9110	T9111	det	any,subpopulation
R4072	T9111	T9109	pobj	subpopulation,from
R4073	T9112	T9111	prep	of,subpopulation
R4074	T9113	T9114	amod	wild,type
R4075	T9114	T9116	compound	type,neurons
R4076	T9115	T9114	punct	-,type
R4077	T9116	T9112	pobj	neurons,of
R4078	T9117	T9116	compound	DRG,neurons
R4079	T9118	T9081	punct	.,suggest
R408	T1765	T1763	nsubj	signaling,controls
R4080	T9120	T9121	advmod	Finally,activated
R4081	T9122	T9121	punct	", ",activated
R4082	T9123	T9124	aux	to,assess
R4083	T9124	T9121	advcl	assess,activated
R4084	T9125	T9126	mark	whether,acts
R4085	T9126	T9124	dobj	acts,assess
R4086	T9127	T9128	compound	EWS,Pea3
R4087	T9128	T9126	nsubj	Pea3,acts
R4088	T9129	T9128	punct	-,Pea3
R4089	T9130	T9128	acl	expressed,Pea3
R409	T1766	T1767	det	the,acquisition
R4090	T9131	T9130	advmod	precociously,expressed
R4091	T9132	T9133	advmod	exclusively,autonomously
R4092	T9133	T9126	advmod	autonomously,acts
R4093	T9134	T9133	npadvmod	cell,autonomously
R4094	T9135	T9133	punct	-,autonomously
R4095	T9136	T9126	cc	or,acts
R4096	T9137	T9138	mark	whether,influence
R4097	T9138	T9126	conj	influence,acts
R4098	T9139	T9138	nsubj	it,influence
R4099	T9140	T9138	aux	may,influence
R410	T1767	T1763	dobj	acquisition,controls
R4100	T9141	T9138	advmod	also,influence
R4101	T9142	T9143	amod	neighboring,neurons
R4102	T9143	T9138	dobj	neurons,influence
R4103	T9144	T9143	compound	DRG,neurons
R4104	T9145	T9121	punct	", ",activated
R4105	T9146	T9121	nsubj	we,activated
R4106	T9147	T9121	dobj	expression,activated
R4107	T9148	T9147	prep	of,expression
R4108	T9149	T9150	compound	EWS,Pea3
R4109	T9150	T9148	pobj	Pea3,of
R411	T1768	T1767	prep	of,acquisition
R4110	T9151	T9150	punct	-,Pea3
R4111	T9152	T9121	advcl	using,activated
R4112	T9153	T9154	compound	Hb9Cre,mice
R4113	T9154	T9152	dobj	mice,using
R4114	T9155	T9156	punct	[,33
R4115	T9156	T9121	parataxis	33,activated
R4116	T9157	T9156	punct	],33
R4117	T9158	T9121	punct	.,activated
R4118	T9160	T9161	prep	Due,undergo
R4119	T9162	T9160	pcomp	to,Due
R412	T1769	T1770	amod	peptidergic,traits
R4120	T9163	T9164	det	a,expression
R4121	T9164	T9160	pobj	expression,Due
R4122	T9165	T9164	amod	transient,expression
R4123	T9166	T9165	cc	and,transient
R4124	T9167	T9168	advmod	rostro,caudally
R4125	T9168	T9170	advmod	caudally,graded
R4126	T9169	T9168	punct	-,caudally
R4127	T9170	T9165	conj	graded,transient
R4128	T9171	T9164	prep	of,expression
R4129	T9172	T9171	pobj	Hb9,of
R413	T1770	T1768	pobj	traits,of
R4130	T9173	T9164	prep	at,expression
R4131	T9174	T9175	amod	neural,plate
R4132	T9175	T9176	compound	plate,stages
R4133	T9176	T9173	pobj	stages,at
R4134	T9177	T9161	punct	", ",undergo
R4135	T9178	T9179	advmod	very,few
R4136	T9179	T9180	amod	few,neurons
R4137	T9180	T9161	nsubj	neurons,undergo
R4138	T9181	T9180	compound	DRG,neurons
R4139	T9182	T9180	prep	at,neurons
R414	T1873	T1870	punct	],12
R4140	T9183	T9184	amod	brachial,levels
R4141	T9184	T9182	pobj	levels,at
R4142	T9185	T9180	cc	and,neurons
R4143	T9186	T9187	advmod	increasingly,more
R4144	T9187	T9188	amod	more,neurons
R4145	T9188	T9180	conj	neurons,neurons
R4146	T9189	T9188	acl	progressing,neurons
R4147	T9190	T9189	advmod	caudally,progressing
R4148	T9191	T9161	dobj	recombination,undergo
R4149	T9192	T9161	prep	in,undergo
R415	T1771	T1767	prep	in,acquisition
R4150	T9193	T9194	nmod	TauEWS,Hb9Cre
R4151	T9194	T9199	nmod	Hb9Cre,embryos
R4152	T9195	T9194	punct	-,Hb9Cre
R4153	T9196	T9194	nmod	Pea3,Hb9Cre
R4154	T9197	T9194	punct	/,Hb9Cre
R4155	T9198	T9194	punct	+,Hb9Cre
R4156	T9199	T9192	pobj	embryos,in
R4157	T9200	T9194	punct	/,Hb9Cre
R4158	T9201	T9194	punct	+,Hb9Cre
R4159	T9202	T9194	cc	and,Hb9Cre
R416	T1772	T1773	amod	nociceptive,neurons
R4160	T9203	T9204	nmod	TaumGFP,Hb9Cre
R4161	T9204	T9194	conj	Hb9Cre,Hb9Cre
R4162	T9205	T9204	punct	/,Hb9Cre
R4163	T9206	T9204	punct	+,Hb9Cre
R4164	T9207	T9204	punct	/,Hb9Cre
R4165	T9208	T9204	punct	+,Hb9Cre
R4166	T9209	T9210	punct	(,Figure
R4167	T9210	T9161	parataxis	Figure,undergo
R4168	T9211	T9210	nummod	8,Figure
R4169	T9212	T9210	punct	),Figure
R417	T1773	T1771	pobj	neurons,in
R4170	T9213	T9161	punct	.,undergo
R4171	T9215	T9216	advmod	Nevertheless,restricted
R4172	T9217	T9216	punct	", ",restricted
R4173	T9218	T9216	nsubjpass	downregulation,restricted
R4174	T9219	T9218	prep	of,downregulation
R4175	T9220	T9221	compound	Trk,expression
R4176	T9221	T9219	pobj	expression,of
R4177	T9222	T9221	compound	receptor,expression
R4178	T9223	T9221	cc	or,expression
R4179	T9224	T9221	conj	upregulation,expression
R418	T1774	T1773	compound	DRG,neurons
R4180	T9225	T9221	prep	of,expression
R4181	T9226	T9225	pobj	Calretinin,of
R4182	T9227	T9216	auxpass	is,restricted
R4183	T9228	T9216	advmod	exclusively,restricted
R4184	T9229	T9216	prep	to,restricted
R4185	T9230	T9229	pobj	neurons,to
R4186	T9231	T9232	dep	that,undergone
R4187	T9232	T9230	relcl	undergone,neurons
R4188	T9233	T9232	aux	have,undergone
R4189	T9234	T9232	dobj	recombination,undergone
R419	T1874	T1846	punct	.,control
R4190	T9235	T9232	cc	and,undergone
R4191	T9236	T9237	aux	can,observed
R4192	T9237	T9232	conj	observed,undergone
R4193	T9238	T9237	neg	not,observed
R4194	T9239	T9237	auxpass	be,observed
R4195	T9240	T9237	prep	in,observed
R4196	T9241	T9242	nmod	TaumGFP,Hb9Cre
R4197	T9242	T9245	nmod	Hb9Cre,embryos
R4198	T9243	T9242	punct	/,Hb9Cre
R4199	T9244	T9242	punct	+,Hb9Cre
R420	T1775	T1767	cc	and,acquisition
R4200	T9245	T9240	pobj	embryos,in
R4201	T9246	T9242	punct	/,Hb9Cre
R4202	T9247	T9242	punct	+,Hb9Cre
R4203	T9248	T9249	punct	(,Figure
R4204	T9249	T9237	parataxis	Figure,observed
R4205	T9250	T9249	nummod	8,Figure
R4206	T9251	T9249	punct	),Figure
R4207	T9252	T9216	punct	.,restricted
R4208	T9254	T9255	advmod	Together,demonstrate
R4209	T9256	T9255	punct	", ",demonstrate
R421	T1776	T1777	det	the,control
R4210	T9257	T9258	det	these,results
R4211	T9258	T9255	nsubj	results,demonstrate
R4212	T9259	T9258	cc	and,results
R4213	T9260	T9261	det	the,findings
R4214	T9261	T9258	conj	findings,results
R4215	T9262	T9261	acl	obtained,findings
R4216	T9263	T9262	prep	from,obtained
R4217	T9264	T9265	advmod	in,vitro
R4218	T9265	T9266	amod	vitro,experiments
R4219	T9266	T9263	pobj	experiments,from
R422	T1876	T1877	prep	In,shown
R4220	T9267	T9266	compound	culture,experiments
R4221	T9268	T9269	punct	(,see
R4222	T9269	T9262	parataxis	see,obtained
R4223	T9270	T9271	nmod	Figures,4
R4224	T9271	T9269	dobj	4,see
R4225	T9272	T9271	cc	and,4
R4226	T9273	T9271	conj	5,4
R4227	T9274	T9269	punct	),see
R4228	T9275	T9276	mark	that,leads
R4229	T9276	T9255	ccomp	leads,demonstrate
R423	T1777	T1767	conj	control,acquisition
R4230	T9277	T9278	amod	precocious,expression
R4231	T9278	T9276	nsubj	expression,leads
R4232	T9279	T9277	cc	or,precocious
R4233	T9280	T9277	conj	isochronic,precocious
R4234	T9281	T9278	prep	of,expression
R4235	T9282	T9283	compound	EWS,Pea3
R4236	T9283	T9281	pobj	Pea3,of
R4237	T9284	T9283	punct	-,Pea3
R4238	T9285	T9278	prep	in,expression
R4239	T9286	T9287	det	the,neurons
R424	T1778	T1777	prep	of,control
R4240	T9287	T9285	pobj	neurons,in
R4241	T9288	T9287	amod	same,neurons
R4242	T9289	T9276	prep	to,leads
R4243	T9290	T9291	advmod	significantly,different
R4244	T9291	T9292	amod	different,responses
R4245	T9292	T9289	pobj	responses,to
R4246	T9293	T9294	npadvmod	cell,autonomous
R4247	T9294	T9292	amod	autonomous,responses
R4248	T9295	T9294	punct	-,autonomous
R4249	T9296	T9292	amod	cellular,responses
R425	T1779	T1780	compound	target,innervation
R4250	T9297	T9276	prep	with,leads
R4251	T9298	T9297	pobj	respect,with
R4252	T9299	T9298	prep	to,respect
R4253	T9300	T9301	compound	gene,expression
R4254	T9301	T9299	pobj	expression,to
R4255	T9302	T9301	punct	", ",expression
R4256	T9303	T9304	amod	neuronal,survival
R4257	T9304	T9301	conj	survival,expression
R4258	T9305	T9304	punct	", ",survival
R4259	T9306	T9304	cc	and,survival
R426	T1780	T1778	pobj	innervation,of
R4260	T9307	T9308	compound	neurite,outgrowth
R4261	T9308	T9304	conj	outgrowth,survival
R4262	T9309	T9310	punct	(,Figure
R4263	T9310	T9255	parataxis	Figure,demonstrate
R4264	T9311	T9310	nummod	9,Figure
R4265	T9312	T9310	punct	),Figure
R4266	T9313	T9255	punct	.,demonstrate
R427	T1878	T1876	pobj	vertebrates,In
R4279	T10766	T10767	npadvmod	Target,derived
R428	T1781	T1782	punct	[,9
R4280	T10767	T10769	amod	derived,signals
R4281	T10768	T10767	punct	-,derived
R4282	T10769	T10770	nsubj	signals,exhibit
R4283	T10771	T10772	det	a,role
R4284	T10772	T10770	dobj	role,exhibit
R4285	T10773	T10772	amod	conserved,role
R4286	T10774	T10772	prep	in,role
R4287	T10775	T10776	det	the,induction
R4288	T10776	T10774	pobj	induction,in
R4289	T10777	T10776	prep	of,induction
R429	T1782	T1763	parataxis	9,controls
R4290	T10778	T10779	amod	defined,programs
R4291	T10779	T10777	pobj	programs,of
R4292	T10780	T10779	prep	of,programs
R4293	T10781	T10782	compound	transcription,factor
R4294	T10782	T10783	compound	factor,expression
R4295	T10783	T10780	pobj	expression,of
R4296	T10784	T10776	amod	late,induction
R4297	T10785	T10784	prep	in,late
R4298	T10786	T10787	amod	post-mitotic,differentiation
R4299	T10787	T10785	pobj	differentiation,in
R430	T1783	T1782	nummod	8,9
R4300	T10788	T10787	amod	neuronal,differentiation
R4301	T10789	T10790	punct	[,10
R4302	T10790	T10770	parataxis	10,exhibit
R4303	T10791	T10790	punct	],10
R4304	T10792	T10770	punct	.,exhibit
R4305	T10794	T10795	det	This,study
R4306	T10795	T10796	nsubj	study,provides
R4307	T10797	T10796	dobj	evidence,provides
R4308	T10798	T10799	mark	that,is
R4309	T10799	T10797	acl	is,evidence
R431	T1879	T1877	punct	", ",shown
R4310	T10800	T10801	det	the,onset
R4311	T10801	T10799	nsubj	onset,is
R4312	T10802	T10801	amod	late,onset
R4313	T10803	T10801	prep	of,onset
R4314	T10804	T10805	compound	transcription,factor
R4315	T10805	T10806	compound	factor,expression
R4316	T10806	T10803	pobj	expression,of
R4317	T10807	T10799	acomp	essential,is
R4318	T10808	T10807	prep	for,essential
R4319	T10809	T10810	amod	many,aspects
R432	T1784	T1782	punct	",",9
R4320	T10810	T10808	pobj	aspects,for
R4321	T10811	T10810	amod	later,aspects
R4322	T10812	T10810	prep	of,aspects
R4323	T10813	T10814	amod	neuronal,differentiation
R4324	T10814	T10812	pobj	differentiation,of
R4325	T10815	T10814	cc	and,differentiation
R4326	T10816	T10817	compound	circuit,formation
R4327	T10817	T10814	conj	formation,differentiation
R4328	T10818	T10796	punct	.,provides
R4329	T10820	T10821	poss	Our,data
R433	T1785	T1782	punct	],9
R4330	T10821	T10822	nsubj	data,indicate
R4331	T10823	T10824	mark	that,undergo
R4332	T10824	T10822	ccomp	undergo,indicate
R4333	T10825	T10826	compound	DRG,neurons
R4334	T10826	T10824	nsubj	neurons,undergo
R4335	T10827	T10828	det	a,change
R4336	T10828	T10824	dobj	change,undergo
R4337	T10829	T10828	amod	temporal,change
R4338	T10830	T10828	prep	in,change
R4339	T10831	T10832	poss	their,competence
R434	T1786	T1756	punct	.,revealed
R4340	T10832	T10830	pobj	competence,in
R4341	T10833	T10834	aux	to,respond
R4342	T10834	T10824	advcl	respond,undergo
R4343	T10835	T10834	prep	to,respond
R4344	T10836	T10837	compound	ETS,signaling
R4345	T10837	T10835	pobj	signaling,to
R4346	T10838	T10839	compound	transcription,factor
R4347	T10839	T10837	compound	factor,signaling
R4348	T10840	T10824	punct	", ",undergo
R4349	T10841	T10842	mark	as,assessed
R435	T1880	T1881	amod	peripheral,signals
R4350	T10842	T10824	advcl	assessed,undergo
R4351	T10843	T10842	prep	by,assessed
R4352	T10844	T10843	pobj	changes,by
R4353	T10845	T10844	prep	in,changes
R4354	T10846	T10847	compound	gene,expression
R4355	T10847	T10845	pobj	expression,in
R4356	T10848	T10847	cc	and,expression
R4357	T10849	T10850	amod	axonal,invasion
R4358	T10850	T10847	conj	invasion,expression
R4359	T10851	T10850	compound	target,invasion
R436	T1881	T1877	nsubjpass	signals,shown
R4360	T10852	T10853	punct	(,Figure
R4361	T10853	T10824	parataxis	Figure,undergo
R4362	T10854	T10853	nummod	9,Figure
R4363	T10855	T10853	punct	),Figure
R4364	T10856	T10822	punct	.,indicate
R4365	T10858	T10859	poss	Our,findings
R4366	T10859	T10860	nsubj	findings,argue
R4367	T10861	T10860	prep	for,argue
R4368	T10862	T10863	det	the,necessity
R4369	T10863	T10861	pobj	necessity,for
R437	T1882	T1881	amod	neurotrophic,signals
R4370	T10864	T10863	prep	of,necessity
R4371	T10865	T10866	npadvmod	target,induced
R4372	T10866	T10868	amod	induced,upregulation
R4373	T10867	T10866	punct	-,induced
R4374	T10868	T10864	pobj	upregulation,of
R4375	T10869	T10866	punct	", ",induced
R4376	T10870	T10866	cc	and,induced
R4377	T10871	T10870	advmod	therefore,and
R4378	T10872	T10873	advmod	temporally,controlled
R4379	T10873	T10866	conj	controlled,induced
R438	T1883	T1877	aux	have,shown
R4380	T10874	T10868	punct	", ",upregulation
R4381	T10875	T10868	prep	of,upregulation
R4382	T10876	T10877	compound	ETS,signaling
R4383	T10877	T10875	pobj	signaling,of
R4384	T10878	T10879	compound	transcription,factor
R4385	T10879	T10877	compound	factor,signaling
R4386	T10880	T10860	punct	.,argue
R4387	T10882	T10883	advmod	More,generally
R4388	T10883	T10884	advmod	generally,suggest
R4389	T10885	T10884	punct	", ",suggest
R439	T1788	T1789	det	The,onset
R4390	T10886	T10884	nsubj	they,suggest
R4391	T10887	T10888	mark	that,represents
R4392	T10888	T10884	ccomp	represents,suggest
R4393	T10889	T10890	advmod	temporally,regulated
R4394	T10890	T10891	amod	regulated,activation
R4395	T10891	T10888	nsubj	activation,represents
R4396	T10892	T10891	prep	of,activation
R4397	T10893	T10894	amod	transcriptional,programs
R4398	T10894	T10892	pobj	programs,of
R4399	T10895	T10891	acl	coupled,activation
R440	T1789	T1790	nsubjpass	onset,shown
R4400	T10896	T10895	prep	to,coupled
R4401	T10897	T10898	det	a,fate
R4402	T10898	T10896	pobj	fate,to
R4403	T10899	T10898	amod	particular,fate
R4404	T10900	T10898	acl	induced,fate
R4405	T10901	T10900	prep	in,induced
R4406	T10902	T10901	pobj	neurons,in
R4407	T10903	T10900	prep	at,induced
R4408	T10904	T10905	amod	early,stages
R4409	T10905	T10903	pobj	stages,at
R441	T1884	T1877	auxpass	been,shown
R4410	T10906	T10905	amod	developmental,stages
R4411	T10907	T10908	det	an,mechanism
R4412	T10908	T10888	dobj	mechanism,represents
R4413	T10909	T10908	amod	important,mechanism
R4414	T10910	T10908	prep	of,mechanism
R4415	T10911	T10912	amod	neuronal,maturation
R4416	T10912	T10910	pobj	maturation,of
R4417	T10913	T10884	punct	.,suggest
R4418	T10915	T10916	nummod	One,observation
R4419	T10916	T10918	nsubj	observation,is
R442	T1791	T1789	prep	of,onset
R4420	T10917	T10916	amod	striking,observation
R4421	T10919	T10916	prep	of,observation
R4422	T10920	T10921	det	this,study
R4423	T10921	T10919	pobj	study,of
R4424	T10922	T10923	mark	that,promotes
R4425	T10923	T10918	ccomp	promotes,is
R4426	T10924	T10925	amod	precocious,induction
R4427	T10925	T10923	nsubj	induction,promotes
R4428	T10926	T10925	prep	of,induction
R4429	T10927	T10928	compound	ETS,signaling
R443	T1792	T1793	det	some,programs
R4430	T10928	T10926	pobj	signaling,of
R4431	T10929	T10930	amod	neuronal,survival
R4432	T10930	T10923	dobj	survival,promotes
R4433	T10931	T10923	prep	without,promotes
R4434	T10932	T10933	det	a,requirement
R4435	T10933	T10931	pobj	requirement,without
R4436	T10934	T10933	prep	for,requirement
R4437	T10935	T10936	amod	neurotrophic,support
R4438	T10936	T10934	pobj	support,for
R4439	T10937	T10931	cc	and,without
R444	T1885	T1886	aux	to,direct
R4440	T10938	T10931	conj	in,without
R4441	T10939	T10940	amod	complete,absence
R4442	T10940	T10938	pobj	absence,in
R4443	T10941	T10940	prep	of,absence
R4444	T10942	T10943	compound	Trk,receptor
R4445	T10943	T10944	compound	receptor,expression
R4446	T10944	T10941	pobj	expression,of
R4447	T10945	T10918	punct	.,is
R4448	T10947	T10948	prep	In,result
R4449	T10949	T10947	pobj	contrast,In
R445	T1793	T1791	pobj	programs,of
R4450	T10950	T10948	punct	", ",result
R4451	T10951	T10952	compound	ETS,signaling
R4452	T10952	T10948	nsubj	signaling,result
R4453	T10953	T10952	prep	at,signaling
R4454	T10954	T10955	det	the,time
R4455	T10955	T10953	pobj	time,at
R4456	T10956	T10955	amod	normal,time
R4457	T10957	T10955	prep	of,time
R4458	T10958	T10957	pobj	onset,of
R4459	T10959	T10958	prep	of,onset
R446	T1794	T1793	amod	transcriptional,programs
R4460	T10960	T10961	compound	Er81,expression
R4461	T10961	T10959	pobj	expression,of
R4462	T10962	T10948	aux	does,result
R4463	T10963	T10948	neg	not,result
R4464	T10964	T10948	prep	in,result
R4465	T10965	T10966	amod	enhanced,survival
R4466	T10966	T10964	pobj	survival,in
R4467	T10967	T10966	amod	neuronal,survival
R4468	T10968	T10948	prep	in,result
R4469	T10969	T10970	det	the,absence
R447	T1795	T1789	prep	in,onset
R4470	T10970	T10968	pobj	absence,in
R4471	T10971	T10970	prep	of,absence
R4472	T10972	T10971	pobj	neurotrophins,of
R4473	T10973	T10948	cc	and,result
R4474	T10974	T10975	advmod	also,lead
R4475	T10975	T10948	conj	lead,result
R4476	T10976	T10975	aux	does,lead
R4477	T10977	T10975	neg	not,lead
R4478	T10978	T10975	prep	to,lead
R4479	T10979	T10978	pobj	downregulation,to
R448	T1886	T1877	xcomp	direct,shown
R4480	T10980	T10979	prep	of,downregulation
R4481	T10981	T10982	compound	TrkC,expression
R4482	T10982	T10980	pobj	expression,of
R4483	T10983	T10979	prep	in,downregulation
R4484	T10984	T10985	amod	proprioceptive,afferents
R4485	T10985	T10983	pobj	afferents,in
R4486	T10986	T10948	punct	.,result
R4487	T10988	T10989	det	These,findings
R4488	T10989	T10990	nsubj	findings,demonstrate
R4489	T10991	T10992	advmod	very,distinct
R449	T1796	T1795	pobj	neurons,in
R4490	T10992	T10993	amod	distinct,activities
R4491	T10993	T10990	dobj	activities,demonstrate
R4492	T10994	T10993	prep	of,activities
R4493	T10995	T10996	nummod	one,regulator
R4494	T10996	T10994	pobj	regulator,of
R4495	T10997	T10996	amod	transcriptional,regulator
R4496	T10998	T10990	prep	at,demonstrate
R4497	T10999	T11000	amod	different,steps
R4498	T11000	T10998	pobj	steps,at
R4499	T11001	T11000	amod	developmental,steps
R450	T1797	T1790	punct	", ",shown
R4500	T11002	T11000	prep	within,steps
R4501	T11003	T11004	det	a,lineage
R4502	T11004	T11002	pobj	lineage,within
R4503	T11005	T11004	amod	committed,lineage
R4504	T11006	T11004	amod	post-mitotic,lineage
R4505	T11007	T11004	amod	neuronal,lineage
R4506	T11008	T10990	punct	.,demonstrate
R4507	T11010	T11011	det	The,absence
R4508	T11011	T11012	nsubj	absence,explain
R4509	T11013	T11011	prep	of,absence
R451	T1887	T1888	det	the,onset
R4510	T11014	T11015	compound	Trk,expression
R4511	T11015	T11013	pobj	expression,of
R4512	T11016	T11015	compound	receptor,expression
R4513	T11017	T11011	prep	upon,absence
R4514	T11018	T11019	amod	precocious,induction
R4515	T11019	T11017	pobj	induction,upon
R4516	T11020	T11019	prep	of,induction
R4517	T11021	T11022	compound	ETS,signaling
R4518	T11022	T11020	pobj	signaling,of
R4519	T11023	T11012	aux	can,explain
R452	T1798	T1790	advmod	however,shown
R4520	T11024	T11025	advmod	only,partially
R4521	T11025	T11012	advmod	partially,explain
R4522	T11026	T11027	det	the,phenotype
R4523	T11027	T11012	dobj	phenotype,explain
R4524	T11028	T11027	amod	observed,phenotype
R4525	T11029	T11012	prep	in,explain
R4526	T11030	T11031	amod	axonal,projections
R4527	T11031	T11029	pobj	projections,in
R4528	T11032	T11012	punct	.,explain
R4529	T11034	T11035	nsubj	Elimination,perturbs
R453	T1799	T1790	punct	", ",shown
R4530	T11036	T11034	prep	of,Elimination
R4531	T11037	T11038	compound	TrkA,receptor
R4532	T11038	T11039	compound	receptor,signaling
R4533	T11039	T11036	pobj	signaling,of
R4534	T11040	T11034	prep	in,Elimination
R4535	T11041	T11042	compound	Bax,mice
R4536	T11042	T11040	pobj	mice,in
R4537	T11043	T11042	compound	mutant,mice
R4538	T11044	T11035	dobj	establishment,perturbs
R4539	T11045	T11044	prep	of,establishment
R454	T1888	T1886	dobj	onset,direct
R4540	T11046	T11047	amod	peripheral,projections
R4541	T11047	T11045	pobj	projections,of
R4542	T11048	T11047	prep	of,projections
R4543	T11049	T11050	amod	cutaneous,afferents
R4544	T11050	T11048	pobj	afferents,of
R4545	T11051	T11035	punct	", ",perturbs
R4546	T11052	T11053	mark	whereas,appear
R4547	T11053	T11035	advcl	appear,perturbs
R4548	T11054	T11053	nsubj	establishment,appear
R4549	T11055	T11054	prep	of,establishment
R455	T1800	T1790	aux	has,shown
R4550	T11056	T11057	amod	central,projections
R4551	T11057	T11055	pobj	projections,of
R4552	T11058	T11053	aux	does,appear
R4553	T11059	T11053	neg	not,appear
R4554	T11060	T11061	aux	to,affected
R4555	T11061	T11053	xcomp	affected,appear
R4556	T11062	T11061	auxpass	be,affected
R4557	T11063	T11064	punct	[,8
R4558	T11064	T11053	parataxis	8,appear
R4559	T11065	T11064	punct	],8
R456	T1801	T1790	advmod	also,shown
R4560	T11066	T11035	punct	.,perturbs
R4561	T11068	T11069	prep	In,perturbed
R4562	T11070	T11071	det	the,absence
R4563	T11071	T11068	pobj	absence,In
R4564	T11072	T11071	prep	of,absence
R4565	T11073	T11074	nmod	NT,signaling
R4566	T11074	T11072	pobj	signaling,of
R4567	T11075	T11073	punct	-,NT
R4568	T11076	T11073	nummod	3,NT
R4569	T11077	T11069	punct	", ",perturbed
R457	T1889	T1888	prep	of,onset
R4570	T11078	T11069	nsubjpass	development,perturbed
R4571	T11079	T11078	prep	of,development
R4572	T11080	T11081	amod	central,projections
R4573	T11081	T11079	pobj	projections,of
R4574	T11082	T11083	advmod	as,as
R4575	T11083	T11080	cc	as,central
R4576	T11084	T11083	advmod	well,as
R4577	T11085	T11080	conj	peripheral,central
R4578	T11086	T11081	amod	proprioceptive,projections
R4579	T11087	T11081	amod	afferent,projections
R458	T1802	T1790	auxpass	been,shown
R4580	T11088	T11069	auxpass	is,perturbed
R4581	T11089	T11090	punct	[,9
R4582	T11090	T11069	parataxis	9,perturbed
R4583	T11091	T11090	punct	],9
R4584	T11092	T11069	punct	.,perturbed
R4585	T11094	T11095	prep	In,found
R4586	T11096	T11094	pobj	contrast,In
R4587	T11097	T11095	punct	", ",found
R4588	T11098	T11095	prep	upon,found
R4589	T11099	T11100	amod	precocious,induction
R459	T1803	T1804	aux	to,occur
R4590	T11100	T11098	pobj	induction,upon
R4591	T11101	T11100	prep	of,induction
R4592	T11102	T11103	compound	ETS,signaling
R4593	T11103	T11101	pobj	signaling,of
R4594	T11104	T11095	punct	", ",found
R4595	T11105	T11095	nsubj	we,found
R4596	T11106	T11107	advmod	more,pronounced
R4597	T11107	T11108	amod	pronounced,defects
R4598	T11108	T11095	dobj	defects,found
R4599	T11109	T11095	prep	in,found
R460	T1804	T1790	xcomp	occur,shown
R4600	T11110	T11111	det	the,establishment
R4601	T11111	T11109	pobj	establishment,in
R4602	T11112	T11111	prep	of,establishment
R4603	T11113	T11114	amod	central,projections
R4604	T11114	T11112	pobj	projections,of
R4605	T11115	T11116	advmod	rather,than
R4606	T11116	T11113	cc	than,central
R4607	T11117	T11113	conj	peripheral,central
R4608	T11118	T11111	prep	for,establishment
R4609	T11119	T11120	det	all,neurons
R461	T1805	T1806	advmod	long,exit
R4610	T11120	T11118	pobj	neurons,for
R4611	T11121	T11120	compound	DRG,neurons
R4612	T11122	T11095	punct	.,found
R4613	T11124	T11125	nsubjpass	Induction,controlled
R4614	T11126	T11124	prep	of,Induction
R4615	T11127	T11128	compound	Er81,expression
R4616	T11128	T11126	pobj	expression,of
R4617	T11129	T11124	prep	in,Induction
R4618	T11130	T11131	amod	proprioceptive,afferents
R4619	T11131	T11129	pobj	afferents,in
R462	T1806	T1804	advcl	exit,occur
R4620	T11132	T11125	auxpass	is,controlled
R4621	T11133	T11125	agent	by,controlled
R4622	T11134	T11135	amod	peripheral,NT
R4623	T11135	T11133	pobj	NT,by
R4624	T11136	T11135	punct	-,NT
R4625	T11137	T11135	nummod	3,NT
R4626	T11138	T11139	mark	as,reach
R4627	T11139	T11125	advcl	reach,controlled
R4628	T11140	T11139	nsubj	axons,reach
R4629	T11141	T11142	det	the,vicinity
R463	T1890	T1889	pobj	expression,of
R4630	T11142	T11139	dobj	vicinity,reach
R4631	T11143	T11142	prep	of,vicinity
R4632	T11144	T11145	compound	target,muscles
R4633	T11145	T11143	pobj	muscles,of
R4634	T11146	T11125	punct	", ",controlled
R4635	T11147	T11125	cc	and,controlled
R4636	T11148	T11149	advmod	thus,occurs
R4637	T11149	T11125	conj	occurs,controlled
R4638	T11150	T11151	advmod	only,d
R4639	T11151	T11154	npadvmod	d,become
R464	T1807	T1806	mark	after,exit
R4640	T11152	T11153	advmod	approximately,3
R4641	T11153	T11151	nummod	3,d
R4642	T11154	T11149	advcl	become,occurs
R4643	T11155	T11154	mark	after,become
R4644	T11156	T11157	amod	proprioceptive,neurons
R4645	T11157	T11154	nsubj	neurons,become
R4646	T11158	T11154	acomp	post-mitotic,become
R4647	T11159	T11160	punct	[,14
R4648	T11160	T11154	parataxis	14,become
R4649	T11161	T11160	nummod	9,14
R465	T1808	T1806	nsubj	neurons,exit
R4650	T11162	T11160	punct	",",14
R4651	T11163	T11160	punct	],14
R4652	T11164	T11125	punct	.,controlled
R4653	T11166	T11167	det	This,upregulation
R4654	T11167	T11174	nsubjpass	upregulation,restricted
R4655	T11168	T11169	advmod	temporally,delayed
R4656	T11169	T11167	amod	delayed,upregulation
R4657	T11170	T11169	cc	and,delayed
R4658	T11171	T11172	npadvmod	target,induced
R4659	T11172	T11169	conj	induced,delayed
R466	T1809	T1810	det	the,cycle
R4660	T11173	T11172	punct	-,induced
R4661	T11175	T11167	prep	of,upregulation
R4662	T11176	T11177	compound	ETS,expression
R4663	T11177	T11175	pobj	expression,of
R4664	T11178	T11179	compound	transcription,factor
R4665	T11179	T11177	compound	factor,expression
R4666	T11180	T11181	amod	several,days
R4667	T11181	T11182	npadvmod	days,emerges
R4668	T11182	T11167	advcl	emerges,upregulation
R4669	T11183	T11182	mark	after,emerges
R467	T1810	T1806	dobj	cycle,exit
R4670	T11184	T11185	det	a,lineage
R4671	T11185	T11182	nsubj	lineage,emerges
R4672	T11186	T11185	amod	neuronal,lineage
R4673	T11187	T11185	prep	of,lineage
R4674	T11188	T11189	det	a,identity
R4675	T11189	T11187	pobj	identity,of
R4676	T11190	T11189	amod	specific,identity
R4677	T11191	T11182	advmod	first,emerges
R4678	T11192	T11174	auxpass	is,restricted
R4679	T11193	T11174	neg	not,restricted
R468	T1891	T1890	prep	of,expression
R4680	T11194	T11174	prep	to,restricted
R4681	T11195	T11196	nmod	DRG,neurons
R4682	T11196	T11194	pobj	neurons,to
R4683	T11197	T11196	amod	sensory,neurons
R4684	T11198	T11174	punct	", ",restricted
R4685	T11199	T11174	cc	but,restricted
R4686	T11200	T11201	auxpass	is,found
R4687	T11201	T11174	conj	found,restricted
R4688	T11202	T11201	advmod	also,found
R4689	T11203	T11201	prep	in,found
R469	T1811	T1810	compound	cell,cycle
R4690	T11204	T11205	compound	motor,neuron
R4691	T11205	T11206	compound	neuron,pools
R4692	T11206	T11203	pobj	pools,in
R4693	T11207	T11208	punct	[,13
R4694	T11208	T11201	parataxis	13,found
R4695	T11209	T11208	punct	],13
R4696	T11210	T11174	punct	.,restricted
R4697	T11212	T11213	npadvmod	Target,derived
R4698	T11213	T11215	amod	derived,factors
R4699	T11214	T11213	punct	-,derived
R470	T1812	T1790	punct	.,shown
R4700	T11215	T11216	nsubjpass	factors,implicated
R4701	T11217	T11216	aux	have,implicated
R4702	T11218	T11216	advmod	also,implicated
R4703	T11219	T11216	auxpass	been,implicated
R4704	T11220	T11216	prep	in,implicated
R4705	T11221	T11220	pcomp	controlling,in
R4706	T11222	T11223	amod	neuronal,maturation
R4707	T11223	T11221	dobj	maturation,controlling
R4708	T11224	T11223	prep	of,maturation
R4709	T11225	T11226	amod	predetermined,neurons
R471	T1892	T1893	det	the,factors
R4710	T11226	T11224	pobj	neurons,of
R4711	T11227	T11221	prep	in,controlling
R4712	T11228	T11227	pobj	Drosophila,in
R4713	T11229	T11228	punct	", ",Drosophila
R4714	T11230	T11231	prep	in,is
R4715	T11231	T11228	relcl	is,Drosophila
R4716	T11232	T11230	pobj	which,in
R4717	T11233	T11231	nsubj	expression,is
R4718	T11234	T11233	prep	of,expression
R4719	T11235	T11234	pobj	members,of
R472	T1814	T1815	det	An,principle
R4720	T11236	T11235	prep	of,members
R4721	T11237	T11238	det	the,family
R4722	T11238	T11236	pobj	family,of
R4723	T11239	T11238	compound	BMP,family
R4724	T11240	T11233	prep	in,expression
R4725	T11241	T11242	det	the,region
R4726	T11242	T11240	pobj	region,in
R4727	T11243	T11242	compound	target,region
R4728	T11244	T11231	acomp	essential,is
R4729	T11245	T11244	prep	for,essential
R473	T1893	T1891	pobj	factors,of
R4730	T11246	T11247	det	the,induction
R4731	T11247	T11245	pobj	induction,for
R4732	T11248	T11247	prep	of,induction
R4733	T11249	T11250	amod	mature,properties
R4734	T11250	T11248	pobj	properties,of
R4735	T11251	T11250	amod	peptidergic,properties
R4736	T11252	T11231	prep	in,is
R4737	T11253	T11254	det	a,subpopulation
R4738	T11254	T11252	pobj	subpopulation,in
R4739	T11255	T11254	prep	of,subpopulation
R474	T1815	T1817	nsubj	principle,is
R4740	T11256	T11255	pobj	neurons,of
R4741	T11257	T11254	acl	marked,subpopulation
R4742	T11258	T11257	agent	by,marked
R4743	T11259	T11260	det	the,expression
R4744	T11260	T11258	pobj	expression,by
R4745	T11261	T11260	compound	coordinate,expression
R4746	T11262	T11260	prep	of,expression
R4747	T11263	T11264	det	the,factors
R4748	T11264	T11262	pobj	factors,of
R4749	T11265	T11264	nummod	two,factors
R475	T1816	T1815	amod	emerging,principle
R4750	T11266	T11264	compound	transcription,factors
R4751	T11267	T11264	appos	Apterous,factors
R4752	T11268	T11267	cc	and,Apterous
R4753	T11269	T11267	conj	Squeeze,Apterous
R4754	T11270	T11271	punct	[,12
R4755	T11271	T11257	parataxis	12,marked
R4756	T11272	T11271	nummod	11,12
R4757	T11273	T11271	punct	",",12
R4758	T11274	T11271	punct	],12
R4759	T11275	T11216	punct	.,implicated
R476	T1894	T1893	compound	ETS,factors
R4760	T11277	T11278	advmod	Thus,appear
R4761	T11279	T11278	punct	", ",appear
R4762	T11280	T11281	preconj	both,in
R4763	T11281	T11278	prep	in,appear
R4764	T11282	T11281	pobj	Drosophila,in
R4765	T11283	T11282	cc	and,Drosophila
R4766	T11284	T11282	conj	vertebrates,Drosophila
R4767	T11285	T11278	punct	", ",appear
R4768	T11286	T11287	npadvmod	target,derived
R4769	T11287	T11289	amod	derived,factors
R477	T1818	T1815	prep	from,principle
R4770	T11288	T11287	punct	-,derived
R4771	T11289	T11278	nsubj	factors,appear
R4772	T11290	T11291	aux	to,act
R4773	T11291	T11278	xcomp	act,appear
R4774	T11292	T11291	advmod	permissively,act
R4775	T11293	T11294	aux	to,induce
R4776	T11294	T11291	advcl	induce,act
R4777	T11295	T11296	det	the,expression
R4778	T11296	T11294	dobj	expression,induce
R4779	T11297	T11296	prep	of,expression
R478	T1819	T1818	pobj	work,from
R4780	T11298	T11299	amod	transcriptional,programs
R4781	T11299	T11297	pobj	programs,of
R4782	T11300	T11299	acl	involved,programs
R4783	T11301	T11300	prep	in,involved
R4784	T11302	T11303	amod	terminal,maturation
R4785	T11303	T11301	pobj	maturation,in
R4786	T11304	T11303	amod	neuronal,maturation
R4787	T11305	T11278	punct	.,appear
R4788	T11307	T11308	poss	Our,findings
R4789	T11308	T11309	nsubj	findings,are
R479	T1820	T1819	prep	in,work
R4790	T11310	T11309	acomp	compatible,are
R4791	T11311	T11310	prep	with,compatible
R4792	T11312	T11313	det	a,model
R4793	T11313	T11311	pobj	model,with
R4794	T11314	T11315	prep	in,acquire
R4795	T11315	T11313	relcl	acquire,model
R4796	T11316	T11314	pobj	which,in
R4797	T11317	T11318	compound	DRG,neurons
R4798	T11318	T11315	nsubj	neurons,acquire
R4799	T11319	T11320	poss	their,fate
R480	T1895	T1893	compound	transcription,factors
R4800	T11320	T11315	dobj	fate,acquire
R4801	T11321	T11320	amod	mature,fate
R4802	T11322	T11315	prep	by,acquire
R4803	T11323	T11324	amod	sequential,addition
R4804	T11324	T11322	pobj	addition,by
R4805	T11325	T11323	cc	and,sequential
R4806	T11326	T11327	advmod	temporally,controlled
R4807	T11327	T11323	conj	controlled,sequential
R4808	T11328	T11324	prep	of,addition
R4809	T11329	T11330	npadvmod	lineage,specific
R481	T1821	T1820	pobj	Drosophila,in
R4810	T11330	T11332	amod	specific,features
R4811	T11331	T11330	punct	-,specific
R4812	T11332	T11328	pobj	features,of
R4813	T11333	T11334	punct	(,Figure
R4814	T11334	T11315	parataxis	Figure,acquire
R4815	T11335	T11334	nummod	9,Figure
R4816	T11336	T11334	punct	),Figure
R4817	T11337	T11309	punct	.,are
R4818	T11339	T11340	npadvmod	Target,derived
R4819	T11340	T11342	amod	derived,factors
R482	T1822	T1821	cc	and,Drosophila
R4820	T11341	T11340	punct	-,derived
R4821	T11342	T11343	nsubj	factors,act
R4822	T11344	T11343	prep	on,act
R4823	T11345	T11346	amod	predetermined,lineages
R4824	T11346	T11344	pobj	lineages,on
R4825	T11347	T11346	amod	neuronal,lineages
R4826	T11348	T11349	aux	to,switch
R4827	T11349	T11343	advcl	switch,act
R4828	T11350	T11351	poss	their,programs
R4829	T11351	T11349	dobj	programs,switch
R483	T1823	T1821	conj	vertebrates,Drosophila
R4830	T11352	T11351	amod	developmental,programs
R4831	T11353	T11354	aux	to,become
R4832	T11354	T11349	advcl	become,switch
R4833	T11355	T11354	acomp	compatible,become
R4834	T11356	T11355	prep	with,compatible
R4835	T11357	T11356	pobj	processes,with
R4836	T11358	T11359	amod	such,as
R4837	T11359	T11357	prep	as,processes
R4838	T11360	T11361	compound	target,invasion
R4839	T11361	T11359	pobj	invasion,as
R484	T1896	T1893	appos	Er81,factors
R4840	T11362	T11361	cc	and,invasion
R4841	T11363	T11361	conj	branching,invasion
R4842	T11364	T11343	punct	.,act
R4843	T11366	T11367	predet	Such,state
R4844	T11367	T11370	nsubjpass	state,accompanied
R4845	T11368	T11367	det	a,state
R4846	T11369	T11367	compound	transition,state
R4847	T11371	T11367	prep	in,state
R4848	T11372	T11373	det	the,acquisition
R4849	T11373	T11371	pobj	acquisition,in
R485	T1824	T1825	mark	that,play
R4850	T11374	T11373	prep	of,acquisition
R4851	T11375	T11376	det	a,fate
R4852	T11376	T11374	pobj	fate,of
R4853	T11377	T11376	amod	defined,fate
R4854	T11378	T11376	amod	neuronal,fate
R4855	T11379	T11370	aux	would,accompanied
R4856	T11380	T11370	auxpass	be,accompanied
R4857	T11381	T11370	agent	by,accompanied
R4858	T11382	T11383	det	the,induction
R4859	T11383	T11381	pobj	induction,by
R486	T1825	T1817	ccomp	play,is
R4860	T11384	T11383	prep	of,induction
R4861	T11385	T11386	amod	appropriate,programs
R4862	T11386	T11384	pobj	programs,of
R4863	T11387	T11386	amod	transcriptional,programs
R4864	T11388	T11383	prep	through,induction
R4865	T11389	T11390	det	the,expression
R4866	T11390	T11388	pobj	expression,through
R4867	T11391	T11390	prep	of,expression
R4868	T11392	T11393	amod	specific,factors
R4869	T11393	T11391	pobj	factors,of
R487	T1826	T1827	npadvmod	target,derived
R4870	T11394	T11393	compound	transcription,factors
R4871	T11395	T11370	punct	.,accompanied
R4872	T11397	T11398	nsubj	Mechanisms,represent
R4873	T11399	T11400	amod	such,as
R4874	T11400	T11397	prep	as,Mechanisms
R4875	T11401	T11402	amod	chromosomal,remodeling
R4876	T11402	T11400	pobj	remodeling,as
R4877	T11403	T11404	dep	that,restrict
R4878	T11404	T11397	relcl	restrict,Mechanisms
R4879	T11405	T11404	cc	or,restrict
R488	T1897	T1896	cc	and,Er81
R4880	T11406	T11404	conj	expand,restrict
R4881	T11407	T11406	dobj	access,expand
R4882	T11408	T11407	prep	to,access
R4883	T11409	T11410	amod	certain,genes
R4884	T11410	T11408	pobj	genes,to
R4885	T11411	T11410	compound	target,genes
R4886	T11412	T11413	punct	[,34
R4887	T11413	T11407	parataxis	34,access
R4888	T11414	T11413	punct	],34
R4889	T11415	T11407	cc	or,access
R489	T1827	T1829	amod	derived,factors
R4890	T11416	T11407	conj	activation,access
R4891	T11417	T11416	prep	by,activation
R4892	T11418	T11417	pobj	cofactors,by
R4893	T11419	T11418	amod	responsible,cofactors
R4894	T11420	T11419	prep	for,responsible
R4895	T11421	T11420	pcomp	changing,for
R4896	T11422	T11423	det	the,action
R4897	T11423	T11421	dobj	action,changing
R4898	T11424	T11423	prep	of,action
R4899	T11425	T11426	amod	particular,factors
R490	T1828	T1827	punct	-,derived
R4900	T11426	T11424	pobj	factors,of
R4901	T11427	T11426	compound	transcription,factors
R4902	T11428	T11429	punct	[,35
R4903	T11429	T11406	parataxis	35,expand
R4904	T11430	T11429	punct	],35
R4905	T11431	T11398	aux	could,represent
R4906	T11432	T11433	amod	possible,mechanisms
R4907	T11433	T11398	dobj	mechanisms,represent
R4908	T11434	T11435	prep	by,shifted
R4909	T11435	T11398	ccomp	shifted,represent
R491	T1829	T1825	nsubj	factors,play
R4910	T11436	T11434	pobj	which,by
R4911	T11437	T11438	det	the,profile
R4912	T11438	T11435	nsubjpass	profile,shifted
R4913	T11439	T11438	amod	downstream,profile
R4914	T11440	T11438	amod	transcriptional,profile
R4915	T11441	T11438	prep	of,profile
R4916	T11442	T11443	det	a,factor
R4917	T11443	T11441	pobj	factor,of
R4918	T11444	T11443	compound	transcription,factor
R4919	T11445	T11435	aux	could,shifted
R492	T1898	T1896	conj	Pea3,Er81
R4920	T11446	T11435	auxpass	be,shifted
R4921	T11447	T11435	advmod	temporally,shifted
R4922	T11448	T11435	prep	towards,shifted
R4923	T11449	T11450	det	the,selection
R4924	T11450	T11448	pobj	selection,towards
R4925	T11451	T11450	cc	and,selection
R4926	T11452	T11450	conj	control,selection
R4927	T11453	T11450	prep	of,selection
R4928	T11454	T11455	amod	distinct,genes
R4929	T11455	T11453	pobj	genes,of
R493	T1830	T1831	det	a,role
R4930	T11456	T11455	compound	target,genes
R4931	T11457	T11398	punct	.,represent
R4932	T11459	T11460	advmod	Interestingly,activated
R4933	T11461	T11460	punct	", ",activated
R4934	T11462	T11463	amod	several,factors
R4935	T11463	T11460	nsubjpass	factors,activated
R4936	T11464	T11463	compound	ETS,factors
R4937	T11465	T11463	compound	transcription,factors
R4938	T11466	T11460	auxpass	are,activated
R4939	T11467	T11460	prep	through,activated
R494	T1831	T1825	dobj	role,play
R4940	T11468	T11467	pobj	release,through
R4941	T11469	T11468	prep	of,release
R4942	T11470	T11469	pobj	autoinhibition,of
R4943	T11471	T11460	prep	via,activated
R4944	T11472	T11471	pobj	interaction,via
R4945	T11473	T11472	prep	with,interaction
R4946	T11474	T11473	pobj	cofactors,with
R4947	T11475	T11471	cc	and,via
R4948	T11476	T11475	punct	/,and
R4949	T11477	T11475	cc	or,and
R495	T1899	T1886	prep	in,direct
R4950	T11478	T11471	conj	via,via
R4951	T11479	T11480	amod	post-translational,modifications
R4952	T11480	T11478	pobj	modifications,via
R4953	T11481	T11482	punct	[,37
R4954	T11482	T11460	parataxis	37,activated
R4955	T11483	T11482	nummod	35,37
R4956	T11484	T11482	punct	",",37
R4957	T11485	T11482	nummod	36,37
R4958	T11486	T11482	punct	",",37
R4959	T11487	T11482	punct	],37
R496	T1832	T1831	amod	crucial,role
R4960	T11488	T11460	punct	.,activated
R4961	T11490	T11491	det	The,fusion
R4962	T11491	T11492	nsubj	fusion,circumvent
R4963	T11493	T11491	prep	of,fusion
R4964	T11494	T11493	pobj	EWS,of
R4965	T11495	T11491	prep	with,fusion
R4966	T11496	T11495	pobj	Pea3,with
R4967	T11497	T11492	aux	could,circumvent
R4968	T11498	T11499	det	a,need
R4969	T11499	T11492	dobj	need,circumvent
R497	T1833	T1825	prep	in,play
R4970	T11500	T11499	prep	for,need
R4971	T11501	T11500	pobj	activation,for
R4972	T11502	T11492	prep	through,circumvent
R4973	T11503	T11504	amod	specific,cofactors
R4974	T11504	T11502	pobj	cofactors,through
R4975	T11505	T11506	mark	while,maintaining
R4976	T11506	T11492	advcl	maintaining,circumvent
R4977	T11507	T11506	advmod	still,maintaining
R4978	T11508	T11509	compound	ETS,dependence
R4979	T11509	T11506	dobj	dependence,maintaining
R498	T1900	T1901	nmod	DRG,neurons
R4980	T11510	T11509	compound	site,dependence
R4981	T11511	T11492	punct	", ",circumvent
R4982	T11512	T11513	advmod	thus,rendering
R4983	T11513	T11492	advcl	rendering,circumvent
R4984	T11514	T11515	compound	EWS,Pea3
R4985	T11515	T11513	dobj	Pea3,rendering
R4986	T11516	T11515	punct	-,Pea3
R4987	T11517	T11518	advmod	less,sensitive
R4988	T11518	T11513	oprd	sensitive,rendering
R4989	T11519	T11518	prep	to,sensitive
R499	T1834	T1835	det	the,induction
R4990	T11520	T11521	det	the,context
R4991	T11521	T11519	pobj	context,to
R4992	T11522	T11521	amod	cellular,context
R4993	T11523	T11518	prep	than,sensitive
R4994	T11524	T11525	amod	endogenous,factors
R4995	T11525	T11523	pobj	factors,than
R4996	T11526	T11525	compound	ETS,factors
R4997	T11527	T11525	compound	transcription,factors
R4998	T11528	T11492	punct	.,circumvent
R4999	T11530	T11531	advcl	Using,demonstrate
R500	T1835	T1833	pobj	induction,in
R5000	T11532	T11533	det	this,protein
R5001	T11533	T11530	dobj	protein,Using
R5002	T11534	T11533	compound	fusion,protein
R5003	T11535	T11531	punct	", ",demonstrate
R5004	T11536	T11537	poss	our,experiments
R5005	T11537	T11531	nsubj	experiments,demonstrate
R5006	T11538	T11539	det	a,change
R5007	T11539	T11531	dobj	change,demonstrate
R5008	T11540	T11539	amod	profound,change
R5009	T11541	T11539	prep	in,change
R501	T1836	T1835	prep	of,induction
R5010	T11542	T11543	det	the,action
R5011	T11543	T11541	pobj	action,in
R5012	T11544	T11543	prep	of,action
R5013	T11545	T11546	compound	ETS,signaling
R5014	T11546	T11544	pobj	signaling,of
R5015	T11547	T11531	prep	at,demonstrate
R5016	T11548	T11549	det	the,level
R5017	T11549	T11547	pobj	level,at
R5018	T11550	T11549	prep	of,level
R5019	T11551	T11552	amod	transcriptional,regulation
R502	T1901	T1899	pobj	neurons,in
R5020	T11552	T11550	pobj	regulation,of
R5021	T11553	T11549	prep	within,level
R5022	T11554	T11555	amod	post-mitotic,neurons
R5023	T11555	T11553	pobj	neurons,within
R5024	T11556	T11555	compound	DRG,neurons
R5025	T11557	T11531	prep	over,demonstrate
R5026	T11558	T11557	pobj	time,over
R5027	T11559	T11531	punct	.,demonstrate
R5028	T11561	T11562	advmod	Moreover,paired
R5029	T11563	T11562	punct	", ",paired
R503	T1837	T1838	det	these,programs
R5030	T11564	T11565	det	the,shift
R5031	T11565	T11562	nsubjpass	shift,paired
R5032	T11566	T11565	amod	observed,shift
R5033	T11567	T11565	amod	transcriptional,shift
R5034	T11568	T11565	prep	in,shift
R5035	T11569	T11570	compound	ETS,signaling
R5036	T11570	T11568	pobj	signaling,in
R5037	T11571	T11562	auxpass	is,paired
R5038	T11572	T11562	prep	with,paired
R5039	T11573	T11574	det	the,onset
R504	T1838	T1836	pobj	programs,of
R5040	T11574	T11572	pobj	onset,with
R5041	T11575	T11574	prep	of,onset
R5042	T11576	T11577	amod	appropriate,regulation
R5043	T11577	T11575	pobj	regulation,of
R5044	T11578	T11577	prep	of,regulation
R5045	T11579	T11580	amod	neuronal,specification
R5046	T11580	T11578	pobj	specification,of
R5047	T11581	T11580	compound	subtype,specification
R5048	T11582	T11577	cc	and,regulation
R5049	T11583	T11577	conj	establishment,regulation
R505	T1902	T1901	amod	sensory,neurons
R5050	T11584	T11583	prep	of,establishment
R5051	T11585	T11586	amod	axonal,projections
R5052	T11586	T11584	pobj	projections,of
R5053	T11587	T11586	prep	into,projections
R5054	T11588	T11589	det	the,area
R5055	T11589	T11587	pobj	area,into
R5056	T11590	T11589	compound	target,area
R5057	T11591	T11562	punct	.,paired
R5058	T11593	T11594	amod	Recent,experiments
R5059	T11594	T11595	nsubj	experiments,adds
R506	T1839	T1838	amod	transcriptional,programs
R5060	T11596	T11594	acl	addressing,experiments
R5061	T11597	T11598	det	the,constraints
R5062	T11598	T11596	dobj	constraints,addressing
R5063	T11599	T11598	amod	temporal,constraints
R5064	T11600	T11598	prep	of,constraints
R5065	T11601	T11602	compound	transcription,factor
R5066	T11602	T11603	compound	factor,action
R5067	T11603	T11600	pobj	action,of
R5068	T11604	T11596	prep	in,addressing
R5069	T11605	T11606	amod	proliferating,cells
R507	T1840	T1841	punct	[,10
R5070	T11606	T11604	pobj	cells,in
R5071	T11607	T11608	amod	neural,progenitor
R5072	T11608	T11606	compound	progenitor,cells
R5073	T11609	T11595	prep	to,adds
R5074	T11610	T11611	det	the,idea
R5075	T11611	T11609	pobj	idea,to
R5076	T11612	T11613	mark	that,are
R5077	T11613	T11611	acl	are,idea
R5078	T11614	T11615	amod	defined,windows
R5079	T11615	T11613	nsubj	windows,are
R508	T1841	T1825	parataxis	10,play
R5080	T11616	T11615	amod	temporal,windows
R5081	T11617	T11615	punct	", ",windows
R5082	T11618	T11619	prep	during,act
R5083	T11619	T11615	relcl	act,windows
R5084	T11620	T11618	pobj	which,during
R5085	T11621	T11622	compound	transcription,factors
R5086	T11622	T11619	nsubj	factors,act
R5087	T11623	T11624	aux	to,control
R5088	T11624	T11619	advcl	control,act
R5089	T11625	T11626	amod	distinct,genes
R509	T1903	T1901	cc	and,neurons
R5090	T11626	T11624	dobj	genes,control
R5091	T11627	T11626	amod	downstream,genes
R5092	T11628	T11626	compound	target,genes
R5093	T11629	T11613	punct	", ",are
R5094	T11630	T11613	prep	of,are
R5095	T11631	T11632	amod	key,importance
R5096	T11632	T11630	pobj	importance,of
R5097	T11633	T11632	prep	to,importance
R5098	T11634	T11635	amod	neuronal,acquisition
R5099	T11635	T11633	pobj	acquisition,to
R510	T1842	T1841	punct	],10
R5100	T11636	T11635	compound	fate,acquisition
R5101	T11637	T11595	punct	.,adds
R5102	T11639	T11640	prep	During,controls
R5103	T11641	T11642	compound	Drosophila,generation
R5104	T11642	T11639	pobj	generation,During
R5105	T11643	T11642	compound	neuroblast,generation
R5106	T11644	T11640	punct	", ",controls
R5107	T11645	T11646	det	the,Hunchback
R5108	T11646	T11640	nsubj	Hunchback,controls
R5109	T11647	T11648	compound	transcription,factor
R511	T1843	T1817	punct	.,is
R5110	T11648	T11646	compound	factor,Hunchback
R5111	T11649	T11640	dobj	specification,controls
R5112	T11650	T11649	cc	and,specification
R5113	T11651	T11649	conj	differentiation,specification
R5114	T11652	T11649	prep	of,specification
R5115	T11653	T11654	advmod	early,born
R5116	T11654	T11656	amod	born,neuroblasts
R5117	T11655	T11654	punct	-,born
R5118	T11656	T11652	pobj	neuroblasts,of
R5119	T11657	T11658	punct	[,38
R512	T1845	T1846	prep	In,control
R5120	T11658	T11640	parataxis	38,controls
R5121	T11659	T11658	punct	],38
R5122	T11660	T11640	punct	.,controls
R5123	T11662	T11663	prep	Over,lose
R5124	T11664	T11662	pobj	time,Over
R5125	T11665	T11663	punct	", ",lose
R5126	T11666	T11663	advmod	however,lose
R5127	T11667	T11663	punct	", ",lose
R5128	T11668	T11663	nsubj	neuroblasts,lose
R5129	T11669	T11663	advmod	progressively,lose
R513	T1904	T1905	compound	motor,pools
R5130	T11670	T11671	poss	their,competence
R5131	T11671	T11663	dobj	competence,lose
R5132	T11672	T11673	aux	to,generate
R5133	T11673	T11671	acl	generate,competence
R5134	T11674	T11673	dobj	cells,generate
R5135	T11675	T11674	prep	of,cells
R5136	T11676	T11677	det	an,fate
R5137	T11677	T11675	pobj	fate,of
R5138	T11678	T11677	amod	early,fate
R5139	T11679	T11673	prep	in,generate
R514	T1847	T1845	pobj	Drosophila,In
R5140	T11680	T11679	pobj	response,in
R5141	T11681	T11680	prep	to,response
R5142	T11682	T11683	compound	Hunchback,expression
R5143	T11683	T11681	pobj	expression,to
R5144	T11684	T11685	punct	[,39
R5145	T11685	T11663	parataxis	39,lose
R5146	T11686	T11685	punct	],39
R5147	T11687	T11663	punct	.,lose
R5148	T11689	T11690	det	These,findings
R5149	T11690	T11691	nsubj	findings,argue
R515	T1848	T1846	punct	", ",control
R5150	T11692	T11691	advmod	thus,argue
R5151	T11693	T11691	advmod	also,argue
R5152	T11694	T11691	prep	for,argue
R5153	T11695	T11696	det	a,change
R5154	T11696	T11694	pobj	change,for
R5155	T11697	T11696	prep	in,change
R5156	T11698	T11699	amod	cellular,competence
R5157	T11699	T11697	pobj	competence,in
R5158	T11700	T11701	aux	to,respond
R5159	T11701	T11699	acl	respond,competence
R516	T1849	T1850	amod	retrograde,signals
R5160	T11702	T11701	prep	to,respond
R5161	T11703	T11704	det	a,factor
R5162	T11704	T11702	pobj	factor,to
R5163	T11705	T11704	amod	specific,factor
R5164	T11706	T11704	compound	transcription,factor
R5165	T11707	T11701	prep	over,respond
R5166	T11708	T11707	pobj	time,over
R5167	T11709	T11710	dep	albeit,in
R5168	T11710	T11701	prep	in,respond
R5169	T11711	T11712	det	an,context
R517	T1905	T1901	conj	pools,neurons
R5170	T11712	T11710	pobj	context,in
R5171	T11713	T11712	amod	early,context
R5172	T11714	T11712	compound	precursor,context
R5173	T11715	T11691	punct	.,argue
R5174	T11717	T11718	advmod	More,generally
R5175	T11718	T11719	advmod	generally,shown
R5176	T11720	T11719	punct	", ",shown
R5177	T11721	T11719	prep	during,shown
R5178	T11732	T11719	advmod	also,shown
R5179	T11722	T11723	det	the,differentiation
R518	T1850	T1846	nsubj	signals,control
R5180	T11733	T11719	auxpass	been,shown
R5181	T11723	T11721	pobj	differentiation,during
R5182	T11734	T11735	aux	to,exhibit
R5183	T11724	T11723	prep	of,differentiation
R5184	T11735	T11719	xcomp	exhibit,shown
R5185	T11736	T11737	amod	distinct,functions
R5186	T11737	T11735	dobj	functions,exhibit
R5187	T11725	T11726	amod	hematopoietic,lineages
R5188	T11738	T11735	prep	at,exhibit
R5189	T11739	T11740	amod	progressive,steps
R519	T1851	T1850	compound	BMP,signals
R5190	T11740	T11738	pobj	steps,at
R5191	T11726	T11724	pobj	lineages,of
R5192	T11741	T11740	prep	of,steps
R5193	T11742	T11743	compound	lineage,specification
R5194	T11743	T11741	pobj	specification,of
R5195	T11727	T11719	punct	", ",shown
R5196	T11744	T11745	punct	[,40
R5197	T11745	T11735	parataxis	40,exhibit
R5198	T11728	T11729	amod	several,factors
R5199	T11746	T11745	punct	],40
R520	T1852	T1850	prep	from,signals
R5200	T11747	T11719	punct	.,shown
R5201	T11749	T11750	nsubj	Analysis,provide
R5202	T11729	T11719	nsubjpass	factors,shown
R5203	T11751	T11749	prep	of,Analysis
R5204	T11752	T11753	det	the,mechanisms
R5205	T11753	T11751	pobj	mechanisms,of
R5206	T11754	T11755	prep	by,shifted
R5207	T11730	T11729	compound	transcription,factors
R5208	T11755	T11753	relcl	shifted,mechanisms
R5209	T11756	T11754	pobj	which,by
R521	T1906	T1905	compound	neuron,pools
R5210	T11731	T11719	aux	have,shown
R5211	T11757	T11758	compound	transcription,factor
R5212	T11758	T11759	compound	factor,programs
R5213	T11759	T11755	nsubjpass	programs,shifted
R5214	T11760	T11755	aux	can,shifted
R5215	T11761	T11755	auxpass	be,shifted
R5216	T11762	T11755	prep	over,shifted
R5217	T11763	T11762	pobj	time,over
R5218	T11764	T11765	aux	to,control
R5219	T11765	T11755	advcl	control,shifted
R522	T1853	T1854	det	the,region
R5220	T11766	T11767	amod	different,complements
R5221	T11767	T11765	dobj	complements,control
R5222	T11768	T11767	prep	of,complements
R5223	T11769	T11770	amod	downstream,genes
R5224	T11770	T11768	pobj	genes,of
R5225	T11771	T11767	cc	and,complements
R5226	T11772	T11771	advmod	thus,and
R5227	T11773	T11774	amod	different,aspects
R5228	T11774	T11767	conj	aspects,complements
R5229	T11775	T11774	prep	of,aspects
R523	T1907	T1908	amod	several,days
R5230	T11776	T11777	amod	neuronal,fates
R5231	T11777	T11775	pobj	fates,of
R5232	T11778	T11776	cc	and,neuronal
R5233	T11779	T11776	conj	cellular,neuronal
R5234	T11780	T11774	prep	in,aspects
R5235	T11781	T11782	compound	progenitor,cells
R5236	T11782	T11780	pobj	cells,in
R5237	T11783	T11782	cc	or,cells
R5238	T11784	T11785	amod	post-mitotic,neurons
R5239	T11785	T11782	conj	neurons,cells
R524	T1854	T1852	pobj	region,from
R5240	T11786	T11750	aux	may,provide
R5241	T11787	T11788	amod	further,insight
R5242	T11788	T11750	dobj	insight,provide
R5243	T11789	T11788	prep	into,insight
R5244	T11790	T11791	det	the,way
R5245	T11791	T11789	pobj	way,into
R5246	T11792	T11793	prep	in,act
R5247	T11793	T11791	relcl	act,way
R5248	T11794	T11792	pobj	which,in
R5249	T11795	T11796	compound	transcription,factors
R525	T1855	T1854	compound	target,region
R5250	T11796	T11793	nsubj	factors,act
R5251	T11797	T11798	aux	to,control
R5252	T11798	T11793	advcl	control,act
R5253	T11799	T11800	det	the,assembly
R5254	T11800	T11798	dobj	assembly,control
R5255	T11801	T11800	prep	of,assembly
R5256	T11802	T11803	amod	neuronal,circuits
R5257	T11803	T11801	pobj	circuits,of
R5258	T11804	T11750	punct	.,provide
R5259	T12346	T12345	prep	of,Generation
R526	T1908	T1909	npadvmod	days,become
R5260	T12347	T12348	amod	transgenic,mice
R5261	T12348	T12346	pobj	mice,of
R5262	T12349	T12345	cc	and,Generation
R5263	T12350	T12351	compound	mouse,genetics
R5264	T12351	T12345	conj	genetics,Generation
R5265	T12353	T12354	compound	Er81EWS,Pea3
R5266	T12354	T12356	compound	Pea3,mice
R5267	T12355	T12354	punct	-,Pea3
R5268	T12356	T12357	nsubjpass	mice,generated
R5269	T12358	T12357	auxpass	were,generated
R527	T1856	T1857	det	the,differentiation
R5270	T12359	T12357	advcl	following,generated
R5271	T12360	T12361	det	a,strategy
R5272	T12361	T12359	dobj	strategy,following
R5273	T12362	T12361	amod	similar,strategy
R5274	T12363	T12362	prep	to,similar
R5275	T12364	T12363	pobj	that,to
R5276	T12365	T12364	acl	described,that
R5277	T12366	T12365	prep	for,described
R5278	T12367	T12368	det	the,generation
R5279	T12368	T12366	pobj	generation,for
R528	T1857	T1846	dobj	differentiation,control
R5280	T12369	T12368	prep	of,generation
R5281	T12370	T12371	compound	Er81NLZ,mice
R5282	T12371	T12369	pobj	mice,of
R5283	T12372	T12373	punct	[,14
R5284	T12373	T12365	parataxis	14,described
R5285	T12374	T12373	punct	],14
R5286	T12375	T12357	punct	.,generated
R5287	T12377	T12378	prep	In,inserted
R5288	T12379	T12377	pobj	brief,In
R5289	T12380	T12378	punct	", ",inserted
R529	T1858	T1857	amod	terminal,differentiation
R5290	T12381	T12382	det	a,vector
R5291	T12382	T12378	nsubjpass	vector,inserted
R5292	T12383	T12382	compound	targeting,vector
R5293	T12384	T12382	prep	with,vector
R5294	T12385	T12386	det	a,coding
R5295	T12386	T12384	pobj	coding,with
R5296	T12387	T12386	compound	cDNA,coding
R5297	T12388	T12382	prep	for,vector
R5298	T12389	T12390	compound	EWS,Pea3
R5299	T12390	T12388	pobj	Pea3,for
R530	T1909	T1886	advcl	become,direct
R5300	T12391	T12390	punct	-,Pea3
R5301	T12392	T12378	auxpass	was,inserted
R5302	T12393	T12378	prep	in,inserted
R5303	T12394	T12393	pobj	frame,in
R5304	T12395	T12394	prep	with,frame
R5305	T12396	T12397	det	the,ATG
R5306	T12397	T12395	pobj	ATG,with
R5307	T12398	T12397	amod	endogenous,ATG
R5308	T12399	T12397	compound	start,ATG
R5309	T12400	T12378	prep	into,inserted
R531	T1859	T1857	prep	of,differentiation
R5310	T12401	T12400	pobj	exon,into
R5311	T12402	T12401	nummod	2,exon
R5312	T12403	T12401	prep	of,exon
R5313	T12404	T12405	det	the,locus
R5314	T12405	T12403	pobj	locus,of
R5315	T12406	T12405	nmod	Er81,locus
R5316	T12407	T12405	amod	genomic,locus
R5317	T12408	T12378	cc	and,inserted
R5318	T12409	T12378	conj	used,inserted
R5319	T12410	T12409	prep	for,used
R532	T1860	T1861	det	a,subpopulation
R5320	T12411	T12412	amod	homologous,recombination
R5321	T12412	T12410	pobj	recombination,for
R5322	T12413	T12409	prep	in,used
R5323	T12414	T12415	compound	W95,cells
R5324	T12415	T12413	pobj	cells,in
R5325	T12416	T12415	compound	ES,cells
R5326	T12417	T12378	punct	.,inserted
R5327	T12419	T12420	compound	EWS,Pea3
R5328	T12420	T12422	nsubj	Pea3,represents
R5329	T12421	T12420	punct	-,Pea3
R533	T1861	T1859	pobj	subpopulation,of
R5330	T12423	T12424	det	a,gene
R5331	T12424	T12422	dobj	gene,represents
R5332	T12425	T12424	compound	fusion,gene
R5333	T12426	T12424	prep	between,gene
R5334	T12427	T12428	det	the,terminal
R5335	T12428	T12426	pobj	terminal,between
R5336	T12429	T12428	compound	amino,terminal
R5337	T12430	T12428	prep	of,terminal
R5338	T12431	T12430	pobj	EWS,of
R5339	T12432	T12428	cc	and,terminal
R534	T1910	T1909	mark	after,become
R5340	T12433	T12434	det	the,domain
R5341	T12434	T12428	conj	domain,terminal
R5342	T12435	T12434	compound	ETS,domain
R5343	T12436	T12434	prep	of,domain
R5344	T12437	T12436	pobj	Pea3,of
R5345	T12438	T12439	punct	[,20
R5346	T12439	T12422	parataxis	20,represents
R5347	T12440	T12439	punct	],20
R5348	T12441	T12422	punct	.,represents
R5349	T12443	T12444	det	The,pair
R535	T1862	T1861	prep	of,subpopulation
R5350	T12444	T12446	nsubj	pair,was
R5351	T12445	T12444	compound	primer,pair
R5352	T12447	T12444	acl	used,pair
R5353	T12448	T12449	aux	to,detect
R5354	T12449	T12447	advcl	detect,used
R5355	T12450	T12449	advmod	specifically,detect
R5356	T12451	T12452	det	the,allele
R5357	T12452	T12449	dobj	allele,detect
R5358	T12453	T12454	compound	Er81EWS,Pea3
R5359	T12454	T12452	compound	Pea3,allele
R536	T1911	T1912	det	these,neurons
R5360	T12455	T12454	punct	-,Pea3
R5361	T12456	T12457	nummod	5,CAGCCACTGCACCTACAAGAC
R5362	T12457	T12446	attr	CAGCCACTGCACCTACAAGAC,was
R5363	T12458	T12456	punct	′,5
R5364	T12459	T12457	punct	-,CAGCCACTGCACCTACAAGAC
R5365	T12460	T12457	punct	-,CAGCCACTGCACCTACAAGAC
R5366	T12461	T12457	nummod	3,CAGCCACTGCACCTACAAGAC
R5367	T12462	T12457	punct	′,CAGCCACTGCACCTACAAGAC
R5368	T12463	T12457	cc	and,CAGCCACTGCACCTACAAGAC
R5369	T12464	T12465	nummod	5,CTTCCTGCTTGATGTCTCCTTC
R537	T1912	T1909	nsubj	neurons,become
R5370	T12465	T12457	conj	CTTCCTGCTTGATGTCTCCTTC,CAGCCACTGCACCTACAAGAC
R5371	T12466	T12464	punct	′,5
R5372	T12467	T12465	punct	-,CTTCCTGCTTGATGTCTCCTTC
R5373	T12468	T12465	punct	-,CTTCCTGCTTGATGTCTCCTTC
R5374	T12469	T12465	nummod	3,CTTCCTGCTTGATGTCTCCTTC
R5375	T12470	T12465	punct	′,CTTCCTGCTTGATGTCTCCTTC
R5376	T12471	T12446	punct	.,was
R5377	T12473	T12474	prep	For,integrated
R5378	T12475	T12476	det	the,generation
R5379	T12476	T12473	pobj	generation,For
R538	T1913	T1909	aux	have,become
R5380	T12477	T12476	prep	of,generation
R5381	T12478	T12479	nmod	TaumGFP,mice
R5382	T12479	T12477	pobj	mice,of
R5383	T12480	T12478	cc	and,TaumGFP
R5384	T12481	T12482	compound	TauEWS,Pea3
R5385	T12482	T12478	conj	Pea3,TaumGFP
R5386	T12483	T12482	punct	-,Pea3
R5387	T12484	T12474	punct	", ",integrated
R5388	T12485	T12486	nmod	lox,pA
R5389	T12486	T12500	nmod	pA,cassettes
R539	T1914	T1909	acomp	post-mitotic,become
R5390	T12487	T12486	punct	-,pA
R5391	T12488	T12486	nmod	STOP,pA
R5392	T12489	T12486	punct	-,pA
R5393	T12490	T12486	nmod	lox,pA
R5394	T12491	T12486	punct	-,pA
R5395	T12492	T12486	nmod	mGFP,pA
R5396	T12493	T12486	punct	-,pA
R5397	T12494	T12486	nmod	IRES,pA
R5398	T12495	T12486	punct	-,pA
R5399	T12496	T12486	nmod	NLS,pA
R540	T1915	T1916	punct	[,16
R5400	T12497	T12486	punct	-,pA
R5401	T12498	T12486	nmod	LacZ,pA
R5402	T12499	T12486	punct	-,pA
R5403	T12500	T12474	nsubjpass	cassettes,integrated
R5404	T12501	T12486	cc	and,pA
R5405	T12502	T12503	compound	lox,pA
R5406	T12503	T12486	conj	pA,pA
R5407	T12504	T12503	punct	-,pA
R5408	T12505	T12503	compound	STOP,pA
R5409	T12506	T12503	punct	-,pA
R541	T1969	T1961	punct	", ",control
R5410	T12507	T12503	compound	lox,pA
R5411	T12508	T12503	punct	-,pA
R5412	T12509	T12503	compound	EWS,pA
R5413	T12510	T12503	punct	-,pA
R5414	T12511	T12503	compound	Pea3,pA
R5415	T12512	T12503	punct	-,pA
R5416	T12513	T12503	compound	IRES,pA
R5417	T12514	T12503	punct	-,pA
R5418	T12515	T12503	compound	NLS,pA
R5419	T12516	T12503	punct	-,pA
R542	T1970	T1961	prep	with,control
R5420	T12517	T12503	compound	LacZ,pA
R5421	T12518	T12503	punct	-,pA
R5422	T12519	T12500	compound	targeting,cassettes
R5423	T12520	T12474	auxpass	were,integrated
R5424	T12521	T12474	prep	into,integrated
R5425	T12522	T12521	pobj	exon,into
R5426	T12523	T12522	nummod	2,exon
R5427	T12524	T12522	prep	of,exon
R5428	T12525	T12526	det	the,locus
R5429	T12526	T12524	pobj	locus,of
R543	T1916	T1877	parataxis	16,shown
R5430	T12527	T12526	nmod	Tau,locus
R5431	T12528	T12526	amod	genomic,locus
R5432	T12529	T12530	punct	(,removed
R5433	T12530	T12474	parataxis	removed,integrated
R5434	T12531	T12532	det	the,ATG
R5435	T12532	T12530	nsubjpass	ATG,removed
R5436	T12533	T12532	amod	endogenous,ATG
R5437	T12534	T12532	compound	start,ATG
R5438	T12535	T12530	auxpass	was,removed
R5439	T12536	T12530	prep	in,removed
R544	T1971	T1972	nsubj	Er81,directing
R5440	T12537	T12538	det	the,vectors
R5441	T12538	T12536	pobj	vectors,in
R5442	T12539	T12538	compound	targeting,vectors
R5443	T12540	T12530	punct	;,removed
R5444	T12541	T12542	nsubj	details,available
R5445	T12542	T12530	amod	available,removed
R5446	T12543	T12542	prep	upon,available
R5447	T12544	T12543	pobj	request,upon
R5448	T12545	T12530	punct	),removed
R5449	T12546	T12474	punct	.,integrated
R545	T1972	T1970	pobj	directing,with
R5450	T12548	T12549	nsubjpass	mGFP,provided
R5451	T12550	T12549	auxpass	was,provided
R5452	T12551	T12549	agent	by,provided
R5453	T12552	T12553	compound	P.,Caroni
R5454	T12553	T12551	pobj	Caroni,by
R5455	T12554	T12555	punct	[,25
R5456	T12555	T12549	parataxis	25,provided
R5457	T12556	T12555	punct	],25
R5458	T12557	T12549	punct	.,provided
R5459	T12559	T12560	compound	ES,recombinants
R546	T1917	T1916	nummod	9,16
R5460	T12560	T12562	nsubjpass	recombinants,screened
R5461	T12561	T12560	compound	cell,recombinants
R5462	T12563	T12562	auxpass	were,screened
R5463	T12564	T12562	prep	by,screened
R5464	T12565	T12566	compound	Southern,blot
R5465	T12566	T12567	compound	blot,analysis
R5466	T12567	T12564	pobj	analysis,by
R5467	T12568	T12562	advcl	using,screened
R5468	T12569	T12570	det	the,probe
R5469	T12570	T12568	dobj	probe,using
R547	T1973	T1974	amod	proprioceptive,neuron
R5470	T12571	T12568	prep	in,using
R5471	T12572	T12573	det	the,region
R5472	T12573	T12571	pobj	region,in
R5473	T12574	T12573	nummod	5,region
R5474	T12575	T12574	punct	′,5
R5475	T12576	T12577	mark	as,described
R5476	T12577	T12568	advcl	described,using
R5477	T12578	T12577	advmod	previously,described
R5478	T12579	T12580	punct	[,41
R5479	T12580	T12577	parataxis	41,described
R548	T1974	T1976	compound	neuron,differentiation
R5480	T12581	T12580	punct	],41
R5481	T12582	T12562	punct	.,screened
R5482	T12584	T12585	nsubj	Frequency,was
R5483	T12586	T12584	prep	of,Frequency
R5484	T12587	T12586	pobj	recombination,of
R5485	T12588	T12587	prep	in,recombination
R5486	T12589	T12590	nummod	129,Ola
R5487	T12590	T12592	compound	Ola,cells
R5488	T12591	T12590	punct	/,Ola
R5489	T12592	T12588	pobj	cells,in
R549	T1975	T1974	amod	sensory,neuron
R5490	T12593	T12592	compound	ES,cells
R5491	T12594	T12595	advmod	approximately,3
R5492	T12595	T12585	attr	3,was
R5493	T12596	T12595	quantmod	1,3
R5494	T12597	T12595	punct	/,3
R5495	T12598	T12585	prep	for,was
R5496	T12599	T12600	det	both,constructs
R5497	T12600	T12598	pobj	constructs,for
R5498	T12601	T12600	compound	Tau,constructs
R5499	T12602	T12585	punct	.,was
R550	T1918	T1916	punct	",",16
R5500	T12604	T12605	prep	For,obtained
R5501	T12606	T12607	det	the,generation
R5502	T12607	T12604	pobj	generation,For
R5503	T12608	T12607	prep	of,generation
R5504	T12609	T12610	compound	PVCre,mice
R5505	T12610	T12608	pobj	mice,of
R5506	T12611	T12605	punct	", ",obtained
R5507	T12612	T12613	nmod	mouse,clones
R5508	T12613	T12605	nsubjpass	clones,obtained
R5509	T12614	T12613	amod	genomic,clones
R551	T1976	T1972	dobj	differentiation,directing
R5510	T12615	T12605	auxpass	were,obtained
R5511	T12616	T12605	prep	by,obtained
R5512	T12617	T12616	pcomp	screening,by
R5513	T12618	T12619	det	a,library
R5514	T12619	T12617	dobj	library,screening
R5515	T12620	T12621	nmod	129SV,J
R5516	T12621	T12619	nmod	J,library
R5517	T12622	T12621	punct	/,J
R5518	T12623	T12619	amod	genomic,library
R5519	T12624	T12625	punct	(,Incyte
R552	T1977	T1972	cc	and,directing
R5520	T12625	T12617	parataxis	Incyte,screening
R5521	T12626	T12625	punct	", ",Incyte
R5522	T12627	T12625	npadvmod	Wilmington,Incyte
R5523	T12628	T12625	punct	", ",Incyte
R5524	T12629	T12625	npadvmod	Delaware,Incyte
R5525	T12630	T12625	punct	", ",Incyte
R5526	T12631	T12632	compound	United,States
R5527	T12632	T12625	npadvmod	States,Incyte
R5528	T12633	T12625	punct	),Incyte
R5529	T12634	T12605	punct	.,obtained
R553	T1919	T1916	nummod	13,16
R5530	T12636	T12637	prep	For,see
R5531	T12638	T12636	pobj	details,For
R5532	T12639	T12638	prep	on,details
R5533	T12640	T12641	det	the,structure
R5534	T12641	T12639	pobj	structure,on
R5535	T12642	T12641	amod	genomic,structure
R5536	T12643	T12641	prep	of,structure
R5537	T12644	T12645	det	the,locus
R5538	T12645	T12643	pobj	locus,of
R5539	T12646	T12645	compound	mouse,locus
R554	T1978	T1979	nsubj	Pea3,directing
R5540	T12647	T12645	compound	PV,locus
R5541	T12648	T12637	punct	[,see
R5542	T12649	T12637	dobj	42,see
R5543	T12650	T12637	punct	],see
R5544	T12651	T12637	punct	.,see
R5545	T12653	T12654	det	An,cassette
R5546	T12654	T12661	nsubjpass	cassette,integrated
R5547	T12655	T12656	compound	IRES,pA
R5548	T12656	T12654	compound	pA,cassette
R5549	T12657	T12656	punct	-,pA
R555	T1979	T1972	conj	directing,directing
R5550	T12658	T12656	compound	Cre,pA
R5551	T12659	T12656	punct	-,pA
R5552	T12660	T12654	compound	targeting,cassette
R5553	T12662	T12663	punct	[,33
R5554	T12663	T12654	parataxis	33,cassette
R5555	T12664	T12663	punct	],33
R5556	T12665	T12661	auxpass	was,integrated
R5557	T12666	T12661	prep	into,integrated
R5558	T12667	T12668	det	the,UTR
R5559	T12668	T12666	pobj	UTR,into
R556	T1920	T1916	punct	",",16
R5560	T12669	T12668	nummod	3,UTR
R5561	T12670	T12669	punct	′,3
R5562	T12671	T12668	prep	of,UTR
R5563	T12672	T12671	pobj	exon,of
R5564	T12673	T12672	nummod	5,exon
R5565	T12674	T12661	punct	", ",integrated
R5566	T12675	T12661	cc	and,integrated
R5567	T12676	T12677	compound	ES,recombinants
R5568	T12677	T12679	nsubjpass	recombinants,screened
R5569	T12678	T12677	compound	cell,recombinants
R557	T1980	T1981	compound	motor,pool
R5570	T12679	T12661	conj	screened,integrated
R5571	T12680	T12679	auxpass	were,screened
R5572	T12681	T12679	prep	with,screened
R5573	T12682	T12683	det	a,probe
R5574	T12683	T12681	pobj	probe,with
R5575	T12684	T12683	nummod	5,probe
R5576	T12685	T12684	punct	′,5
R5577	T12686	T12687	punct	(,digest
R5578	T12687	T12679	parataxis	digest,screened
R5579	T12688	T12687	dep	oligos,digest
R558	T1981	T1983	compound	pool,differentiation
R5580	T12689	T12688	punct	", ",oligos
R5581	T12690	T12691	nummod	5,GAGATGACCCAGCCAGGATGCCTC
R5582	T12691	T12688	conj	GAGATGACCCAGCCAGGATGCCTC,oligos
R5583	T12692	T12690	punct	′,5
R5584	T12693	T12691	punct	-,GAGATGACCCAGCCAGGATGCCTC
R5585	T12694	T12691	punct	-,GAGATGACCCAGCCAGGATGCCTC
R5586	T12695	T12691	nummod	3,GAGATGACCCAGCCAGGATGCCTC
R5587	T12696	T12691	punct	′,GAGATGACCCAGCCAGGATGCCTC
R5588	T12697	T12691	cc	and,GAGATGACCCAGCCAGGATGCCTC
R5589	T12698	T12699	nummod	5,CTGACCACTCTCGCTCCGGTGTCC
R559	T1982	T1981	compound	neuron,pool
R5590	T12699	T12691	conj	CTGACCACTCTCGCTCCGGTGTCC,GAGATGACCCAGCCAGGATGCCTC
R5591	T12700	T12698	punct	′,5
R5592	T12701	T12699	punct	-,CTGACCACTCTCGCTCCGGTGTCC
R5593	T12702	T12699	punct	-,CTGACCACTCTCGCTCCGGTGTCC
R5594	T12703	T12699	nummod	3,CTGACCACTCTCGCTCCGGTGTCC
R5595	T12704	T12699	punct	′,CTGACCACTCTCGCTCCGGTGTCC
R5596	T12705	T12687	punct	;,digest
R5597	T12706	T12707	amod	genomic,DNA
R5598	T12707	T12687	dep	DNA,digest
R5599	T12708	T12687	punct	", ",digest
R560	T1921	T1916	nummod	14,16
R5600	T12709	T12687	compound	HindIII,digest
R5601	T12710	T12687	punct	),digest
R5602	T12711	T12679	punct	.,screened
R5603	T12713	T12714	det	The,frequency
R5604	T12714	T12715	nsubj	frequency,was
R5605	T12716	T12714	prep	of,frequency
R5606	T12717	T12716	pobj	recombination,of
R5607	T12718	T12717	prep	in,recombination
R5608	T12719	T12720	nummod	129,Ola
R5609	T12720	T12722	compound	Ola,cells
R561	T1983	T1979	dobj	differentiation,directing
R5610	T12721	T12720	punct	/,Ola
R5611	T12722	T12718	pobj	cells,in
R5612	T12723	T12722	compound	ES,cells
R5613	T12724	T12725	advmod	approximately,20
R5614	T12725	T12715	attr	20,was
R5615	T12726	T12725	quantmod	1,20
R5616	T12727	T12725	punct	/,20
R5617	T12728	T12715	punct	.,was
R5618	T12730	T12731	amod	Recombinant,clones
R5619	T12731	T12732	nsubjpass	clones,aggregated
R562	T1984	T1972	punct	", ",directing
R5620	T12733	T12732	auxpass	were,aggregated
R5621	T12734	T12732	prep	with,aggregated
R5622	T12735	T12736	compound	morula,stage
R5623	T12736	T12737	compound	stage,embryos
R5624	T12737	T12734	pobj	embryos,with
R5625	T12738	T12739	aux	to,generate
R5626	T12739	T12732	advcl	generate,aggregated
R5627	T12740	T12741	amod	chimeric,mice
R5628	T12741	T12739	dobj	mice,generate
R5629	T12742	T12741	compound	founder,mice
R563	T1922	T1916	punct	",",16
R5630	T12743	T12744	dep	that,transmitted
R5631	T12744	T12741	relcl	transmitted,mice
R5632	T12745	T12746	det	the,alleles
R5633	T12746	T12744	dobj	alleles,transmitted
R5634	T12747	T12746	compound	mutant,alleles
R5635	T12748	T12732	punct	.,aggregated
R5636	T12750	T12751	prep	In,were
R5637	T12752	T12753	det	all,experiments
R5638	T12753	T12750	pobj	experiments,In
R5639	T12754	T12753	acl	performed,experiments
R564	T1985	T1972	advmod	respectively,directing
R5640	T12755	T12754	prep	in,performed
R5641	T12756	T12757	det	this,study
R5642	T12757	T12755	pobj	study,in
R5643	T12758	T12751	punct	", ",were
R5644	T12759	T12751	nsubj	animals,were
R5645	T12760	T12751	prep	of,were
R5646	T12761	T12762	amod	mixed,background
R5647	T12762	T12760	pobj	background,of
R5648	T12763	T12762	amod	genetic,background
R5649	T12764	T12765	punct	(,Ola
R565	T1986	T1987	punct	[,15
R5650	T12765	T12751	parataxis	Ola,were
R5651	T12766	T12765	nummod	129,Ola
R5652	T12767	T12765	punct	/,Ola
R5653	T12768	T12765	cc	and,Ola
R5654	T12769	T12765	conj	C57Bl6,Ola
R5655	T12770	T12765	punct	),Ola
R5656	T12771	T12751	punct	.,were
R5657	T12773	T12774	nmod	Thy1spGFP,mice
R5658	T12774	T12776	nsubjpass	mice,generated
R5659	T12775	T12774	amod	transgenic,mice
R566	T1987	T1961	parataxis	15,control
R5660	T12777	T12776	auxpass	were,generated
R5661	T12778	T12776	prep	in,generated
R5662	T12779	T12778	pobj	analogy,in
R5663	T12780	T12779	prep	to,analogy
R5664	T12781	T12782	compound	De,Paola
R5665	T12782	T12780	pobj	Paola,to
R5666	T12783	T12784	advmod	et,al.
R5667	T12784	T12782	advmod	al.,Paola
R5668	T12785	T12786	punct	[,25
R5669	T12786	T12776	parataxis	25,generated
R567	T1988	T1987	nummod	14,15
R5670	T12787	T12786	punct	],25
R5671	T12788	T12776	punct	", ",generated
R5672	T12789	T12776	cc	and,generated
R5673	T12790	T12791	prep	for,selected
R5674	T12791	T12776	conj	selected,generated
R5675	T12792	T12793	det	these,experiments
R5676	T12793	T12790	pobj	experiments,for
R5677	T12794	T12795	det	a,strain
R5678	T12795	T12791	nsubjpass	strain,selected
R5679	T12796	T12795	prep	of,strain
R568	T1989	T1987	punct	",",15
R5680	T12797	T12796	pobj	mice,of
R5681	T12798	T12797	prep	with,mice
R5682	T12799	T12800	amod	early,expression
R5683	T12800	T12798	pobj	expression,with
R5684	T12801	T12800	amod	embryonic,expression
R5685	T12802	T12791	auxpass	was,selected
R5686	T12803	T12791	punct	.,selected
R5687	T12805	T12806	nmod	Isl1Cre,strains
R5688	T12806	T12810	nsubjpass	strains,described
R5689	T12807	T12805	cc	and,Isl1Cre
R569	T1990	T1987	punct	],15
R5690	T12808	T12805	conj	Hb9Cre,Isl1Cre
R5691	T12809	T12806	compound	mouse,strains
R5692	T12811	T12810	aux	have,described
R5693	T12812	T12810	auxpass	been,described
R5694	T12813	T12814	punct	[,43
R5695	T12814	T12810	parataxis	43,described
R5696	T12815	T12814	nummod	33,43
R5697	T12816	T12814	punct	",",43
R5698	T12817	T12814	punct	],43
R5699	T12818	T12810	cc	and,described
R570	T1923	T1916	nummod	15,16
R5700	T12819	T12820	nmod	Bax,animals
R5701	T12820	T12824	nsubj	animals,were
R5702	T12821	T12819	punct	+,Bax
R5703	T12822	T12819	punct	/,Bax
R5704	T12823	T12819	punct	−,Bax
R5705	T12824	T12810	conj	were,described
R5706	T12825	T12824	prep	from,were
R5707	T12826	T12827	compound	Jackson,Laboratory
R5708	T12827	T12825	pobj	Laboratory,from
R5709	T12828	T12829	punct	(,Harbor
R571	T1991	T1961	punct	.,control
R5710	T12829	T12824	parataxis	Harbor,were
R5711	T12830	T12829	compound	Bar,Harbor
R5712	T12831	T12829	punct	", ",Harbor
R5713	T12832	T12829	npadvmod	Maine,Harbor
R5714	T12833	T12829	punct	", ",Harbor
R5715	T12834	T12835	compound	United,States
R5716	T12835	T12829	npadvmod	States,Harbor
R5717	T12836	T12829	punct	),Harbor
R5718	T12837	T12838	punct	[,27
R5719	T12838	T12824	parataxis	27,were
R572	T1993	T1994	prep	In,fail
R5720	T12839	T12838	punct	],27
R5721	T12840	T12824	punct	.,were
R5722	T12842	T12843	amod	Timed,pregnancies
R5723	T12843	T12844	nsubjpass	pregnancies,set
R5724	T12845	T12844	auxpass	were,set
R5725	T12846	T12844	prt	up,set
R5726	T12847	T12848	aux	to,generate
R5727	T12848	T12844	advcl	generate,set
R5728	T12849	T12848	dobj	embryos,generate
R5729	T12850	T12849	prep	of,embryos
R573	T1924	T1916	punct	",",16
R5730	T12851	T12852	amod	different,stages
R5731	T12852	T12850	pobj	stages,of
R5732	T12853	T12852	amod	developmental,stages
R5733	T12854	T12855	mark	with,described
R5734	T12855	T12844	advcl	described,set
R5735	T12856	T12857	det	all,genotypes
R5736	T12857	T12855	nsubj	genotypes,described
R5737	T12858	T12855	prep	throughout,described
R5738	T12859	T12860	det	the,study
R5739	T12860	T12858	pobj	study,throughout
R574	T1995	T1993	amod	particular,In
R5740	T12861	T12844	punct	.,set
R5741	T13099	T13100	amod	Transcriptional,transactivation
R5742	T13100	T13101	compound	transactivation,assays
R5743	T13103	T13104	det	The,plasmids
R5744	T13104	T13106	nsubjpass	plasmids,used
R5745	T13105	T13104	amod	following,plasmids
R5746	T13107	T13106	auxpass	were,used
R5747	T13108	T13106	prep	for,used
R5748	T13109	T13110	amod	transcriptional,assays
R5749	T13110	T13108	pobj	assays,for
R575	T1996	T1994	punct	", ",fail
R5750	T13111	T13110	compound	transactivation,assays
R5751	T13112	T13106	punct	: ,used
R5752	T13113	T13114	compound	pRc,RSV
R5753	T13114	T13106	dobj	RSV,used
R5754	T13115	T13114	punct	/,RSV
R5755	T13116	T13117	punct	(,Invitrogen
R5756	T13117	T13114	parataxis	Invitrogen,RSV
R5757	T13118	T13117	punct	", ",Invitrogen
R5758	T13119	T13117	npadvmod	Carlsbad,Invitrogen
R5759	T13120	T13117	punct	", ",Invitrogen
R576	T1925	T1916	punct	],16
R5760	T13121	T13117	npadvmod	California,Invitrogen
R5761	T13122	T13117	punct	", ",Invitrogen
R5762	T13123	T13124	compound	United,States
R5763	T13124	T13117	npadvmod	States,Invitrogen
R5764	T13125	T13117	punct	),Invitrogen
R5765	T13126	T13114	punct	", ",RSV
R5766	T13127	T13128	compound	pRc,RSV
R5767	T13128	T13130	compound	RSV,Er81
R5768	T13129	T13128	punct	/,RSV
R5769	T13130	T13114	conj	Er81,RSV
R577	T1997	T1994	prep	in,fail
R5770	T13131	T13130	punct	-,Er81
R5771	T13132	T13130	punct	", ",Er81
R5772	T13133	T13134	compound	pRc,RSV
R5773	T13134	T13136	compound	RSV,Pea3
R5774	T13135	T13134	punct	/,RSV
R5775	T13136	T13130	conj	Pea3,Er81
R5776	T13137	T13136	punct	-,Pea3
R5777	T13138	T13136	compound	EWS,Pea3
R5778	T13139	T13136	punct	-,Pea3
R5779	T13140	T13136	punct	", ",Pea3
R578	T1998	T1999	det	the,absence
R5780	T13141	T13142	compound	pTP,5xETS
R5781	T13142	T13136	conj	5xETS,Pea3
R5782	T13143	T13142	punct	-,5xETS
R5783	T13144	T13142	punct	", ",5xETS
R5784	T13145	T13142	cc	and,5xETS
R5785	T13146	T13147	compound	pTP,5xETSmut
R5786	T13147	T13142	conj	5xETSmut,5xETS
R5787	T13148	T13147	punct	-,5xETSmut
R5788	T13149	T13106	punct	.,used
R5789	T13151	T13152	compound	pRc,RSV
R579	T1926	T1877	punct	.,shown
R5790	T13152	T13154	compound	RSV,Er81
R5791	T13153	T13152	punct	/,RSV
R5792	T13154	T13156	nsubjpass	Er81,obtained
R5793	T13155	T13154	punct	-,Er81
R5794	T13157	T13154	cc	and,Er81
R5795	T13158	T13159	compound	pRc,RSV
R5796	T13159	T13161	compound	RSV,Pea3
R5797	T13160	T13159	punct	/,RSV
R5798	T13161	T13154	conj	Pea3,Er81
R5799	T13162	T13161	punct	-,Pea3
R580	T1999	T1997	pobj	absence,in
R5800	T13163	T13161	compound	EWS,Pea3
R5801	T13164	T13161	punct	-,Pea3
R5802	T13165	T13156	auxpass	were,obtained
R5803	T13166	T13156	prep	by,obtained
R5804	T13167	T13166	pobj	insertion,by
R5805	T13168	T13167	prep	of,insertion
R5806	T13169	T13170	det	the,cDNAs
R5807	T13170	T13168	pobj	cDNAs,of
R5808	T13171	T13170	prep	for,cDNAs
R5809	T13172	T13171	pobj	Er81,for
R581	T2000	T1999	prep	of,absence
R5810	T13173	T13172	cc	or,Er81
R5811	T13174	T13175	compound	EWS,Pea3
R5812	T13175	T13172	conj	Pea3,Er81
R5813	T13176	T13175	punct	-,Pea3
R5814	T13177	T13178	punct	(,gift
R5815	T13178	T13175	parataxis	gift,Pea3
R5816	T13179	T13178	prep	from,gift
R5817	T13180	T13181	compound	J.,Hassell
R5818	T13181	T13179	pobj	Hassell,from
R5819	T13182	T13181	compound	A.,Hassell
R582	T2001	T2000	pobj	Er81,of
R5820	T13183	T13178	punct	),gift
R5821	T13184	T13167	prep	into,insertion
R5822	T13185	T13186	compound	pRc,RSV
R5823	T13186	T13184	pobj	RSV,into
R5824	T13187	T13186	punct	/,RSV
R5825	T13188	T13156	punct	.,obtained
R5826	T13190	T13191	compound	pTP,5xETS
R5827	T13191	T13193	nsubjpass	5xETS,constructed
R5828	T13192	T13191	punct	-,5xETS
R5829	T13194	T13193	auxpass	was,constructed
R583	T1928	T1929	advmod	Moreover,known
R5830	T13195	T13193	prep	by,constructed
R5831	T13196	T13195	pcomp	inserting,by
R5832	T13197	T13198	det	a,cassette
R5833	T13198	T13196	dobj	cassette,inserting
R5834	T13199	T13198	prep	of,cassette
R5835	T13200	T13201	nummod	five,copies
R5836	T13201	T13199	pobj	copies,of
R5837	T13202	T13201	amod	repetitive,copies
R5838	T13203	T13201	prep	of,copies
R5839	T13204	T13205	amod	high,affinity
R584	T2002	T1999	punct	", ",absence
R5840	T13205	T13207	compound	affinity,sites
R5841	T13206	T13205	punct	-,affinity
R5842	T13207	T13203	pobj	sites,of
R5843	T13208	T13207	compound	Pea3,sites
R5844	T13209	T13207	compound	binding,sites
R5845	T13210	T13211	punct	(,3
R5846	T13211	T13207	parataxis	3,sites
R5847	T13212	T13211	dep	5,3
R5848	T13213	T13212	punct	′,5
R5849	T13214	T13211	punct	-,3
R585	T2003	T1999	acl	achieved,absence
R5850	T13215	T13211	dep	GCCGGAAGC,3
R5851	T13216	T13211	punct	-,3
R5852	T13217	T13211	punct	′,3
R5853	T13218	T13211	punct	),3
R5854	T13219	T13220	punct	[,19
R5855	T13220	T13207	parataxis	19,sites
R5856	T13221	T13220	nummod	18,19
R5857	T13222	T13220	punct	",",19
R5858	T13223	T13220	punct	],19
R5859	T13224	T13196	prep	into,inserting
R586	T2004	T2003	prep	by,achieved
R5860	T13225	T13226	det	a,version
R5861	T13226	T13224	pobj	version,into
R5862	T13227	T13226	amod	modified,version
R5863	T13228	T13226	prep	of,version
R5864	T13229	T13230	compound	pTK,Luc
R5865	T13230	T13228	pobj	Luc,of
R5866	T13231	T13230	punct	-,Luc
R5867	T13232	T13193	punct	.,constructed
R5868	T13234	T13235	compound	pTP,5xETSmut
R5869	T13235	T13237	nsubjpass	5xETSmut,generated
R587	T2005	T2004	pobj	mutation,by
R5870	T13236	T13235	punct	-,5xETSmut
R5871	T13238	T13237	auxpass	was,generated
R5872	T13239	T13237	prep	as,generated
R5873	T13240	T13241	compound	pTP,5xETS
R5874	T13241	T13239	pobj	5xETS,as
R5875	T13242	T13241	punct	-,5xETS
R5876	T13243	T13239	cc	but,as
R5877	T13244	T13239	conj	using,as
R5878	T13245	T13246	det	a,complement
R5879	T13246	T13244	dobj	complement,using
R588	T1930	T1929	punct	", ",known
R5880	T13247	T13246	amod	mutated,complement
R5881	T13248	T13246	prep	of,complement
R5882	T13249	T13250	det	the,sites
R5883	T13250	T13248	pobj	sites,of
R5884	T13251	T13250	compound	Pea3,sites
R5885	T13252	T13250	compound	binding,sites
R5886	T13253	T13254	punct	(,3
R5887	T13254	T13246	parataxis	3,complement
R5888	T13255	T13254	dep	5,3
R5889	T13256	T13255	punct	′,5
R589	T2006	T2005	prep	in,mutation
R5890	T13257	T13254	punct	-,3
R5891	T13258	T13254	dep	GCCTATGGC,3
R5892	T13259	T13254	punct	-,3
R5893	T13260	T13254	punct	′,3
R5894	T13261	T13254	punct	),3
R5895	T13262	T13237	punct	.,generated
R5896	T13264	T13265	det	A,plasmid
R5897	T13265	T13267	nsubj	plasmid,were
R5898	T13266	T13265	compound	control,plasmid
R5899	T13268	T13269	aux	to,normalize
R590	T2007	T2008	det	the,gene
R5900	T13269	T13265	advcl	normalize,plasmid
R5901	T13270	T13269	prep	for,normalize
R5902	T13271	T13272	compound	transfection,efficiency
R5903	T13272	T13270	pobj	efficiency,for
R5904	T13273	T13274	punct	(,placZ
R5905	T13274	T13272	parataxis	placZ,efficiency
R5906	T13275	T13274	punct	),placZ
R5907	T13276	T13272	cc	and,efficiency
R5908	T13277	T13278	compound	pTK,Luc
R5909	T13278	T13272	conj	Luc,efficiency
R591	T2008	T2006	pobj	gene,in
R5910	T13279	T13278	punct	-,Luc
R5911	T13280	T13281	det	a,gift
R5912	T13281	T13267	attr	gift,were
R5913	T13282	T13281	prep	from,gift
R5914	T13283	T13284	compound	D.,Kressler
R5915	T13284	T13282	pobj	Kressler,from
R5916	T13285	T13267	punct	.,were
R5917	T13287	T13288	nmod	COS,cells
R5918	T13288	T13291	nsubjpass	cells,co-transfected
R5919	T13289	T13287	punct	-,COS
R592	T2009	T2004	cc	or,by
R5920	T13290	T13287	nummod	7,COS
R5921	T13292	T13291	auxpass	were,co-transfected
R5922	T13293	T13291	prep	with,co-transfected
R5923	T13294	T13295	quantmod	1,1.2
R5924	T13295	T13297	nummod	1.2,μg
R5925	T13296	T13295	punct	–,1.2
R5926	T13297	T13293	pobj	μg,with
R5927	T13298	T13297	prep	of,μg
R5928	T13299	T13300	amod	total,DNA
R5929	T13300	T13298	pobj	DNA,of
R593	T1931	T1932	det	the,induction
R5930	T13301	T13297	prep	including,μg
R5931	T13302	T13301	pobj	one,including
R5932	T13303	T13302	prep	of,one
R5933	T13304	T13305	det	the,plasmids
R5934	T13305	T13303	pobj	plasmids,of
R5935	T13306	T13305	compound	effector,plasmids
R5936	T13307	T13308	compound	pRc,RSV
R5937	T13308	T13302	appos	RSV,one
R5938	T13309	T13308	punct	/,RSV
R5939	T13310	T13308	punct	-,RSV
R594	T2010	T2004	conj	by,by
R5940	T13311	T13308	amod	empty,RSV
R5941	T13312	T13308	punct	", ",RSV
R5942	T13313	T13314	compound	pRc,RSV
R5943	T13314	T13316	compound	RSV,Er81
R5944	T13315	T13314	punct	/,RSV
R5945	T13316	T13308	conj	Er81,RSV
R5946	T13317	T13316	punct	-,Er81
R5947	T13318	T13316	punct	", ",Er81
R5948	T13319	T13316	cc	or,Er81
R5949	T13320	T13321	compound	pRc,RSV
R595	T2011	T2010	pobj	deprivation,by
R5950	T13321	T13323	compound	RSV,Pea3
R5951	T13322	T13321	punct	/,RSV
R5952	T13323	T13316	conj	Pea3,Er81
R5953	T13324	T13323	punct	-,Pea3
R5954	T13325	T13323	compound	EWS,Pea3
R5955	T13326	T13323	punct	-,Pea3
R5956	T13327	T13302	punct	;,one
R5957	T13328	T13302	conj	one,one
R5958	T13329	T13328	prep	of,one
R5959	T13330	T13331	det	the,plasmids
R596	T1932	T1929	nsubjpass	induction,known
R5960	T13331	T13329	pobj	plasmids,of
R5961	T13332	T13331	compound	reporter,plasmids
R5962	T13333	T13334	compound	pTP,5xETS
R5963	T13334	T13328	appos	5xETS,one
R5964	T13335	T13334	punct	-,5xETS
R5965	T13336	T13334	cc	or,5xETS
R5966	T13337	T13338	compound	pTP,5xETSmut
R5967	T13338	T13334	conj	5xETSmut,5xETS
R5968	T13339	T13338	punct	-,5xETSmut
R5969	T13340	T13328	punct	;,one
R597	T2012	T2011	prep	of,deprivation
R5970	T13341	T13328	cc	and,one
R5971	T13342	T13328	conj	placZ,one
R5972	T13343	T13291	punct	.,co-transfected
R5973	T13345	T13346	nsubjpass	Cells,harvested
R5974	T13347	T13346	auxpass	were,harvested
R5975	T13348	T13346	prep	after,harvested
R5976	T13349	T13350	nummod	25,h
R5977	T13350	T13348	pobj	h,after
R5978	T13351	T13346	cc	and,harvested
R5979	T13352	T13346	conj	processed,harvested
R598	T2013	T2014	amod	peripheral,signaling
R5980	T13353	T13352	prep	for,processed
R5981	T13354	T13353	pobj	assays,for
R5982	T13355	T13356	aux	to,determine
R5983	T13356	T13352	advcl	determine,processed
R5984	T13357	T13358	nmod	luciferase,activity
R5985	T13358	T13356	dobj	activity,determine
R5986	T13359	T13357	cc	and,luciferase
R5987	T13360	T13357	conj	LacZ,luciferase
R5988	T13361	T13362	mark	as,described
R5989	T13362	T13356	advcl	described,determine
R599	T1933	T1932	prep	of,induction
R5990	T13363	T13362	advmod	previously,described
R5991	T13364	T13365	punct	[,44
R5992	T13365	T13352	parataxis	44,processed
R5993	T13366	T13365	punct	],44
R5994	T13367	T13346	punct	.,harvested
R5995	T13369	T13370	compound	Luciferase,values
R5996	T13370	T13371	nsubjpass	values,referred
R5997	T13372	T13370	acl	normalized,values
R5998	T13373	T13372	prep	to,normalized
R5999	T13374	T13375	compound	LacZ,activity
R600	T2014	T2012	pobj	signaling,of
R6000	T13375	T13373	pobj	activity,to
R6001	T13376	T13371	auxpass	are,referred
R6002	T13377	T13371	prep	to,referred
R6003	T13378	T13371	prep	as,referred
R6004	T13379	T13380	compound	luciferase,units
R6005	T13380	T13378	pobj	units,as
R6006	T13381	T13371	punct	.,referred
R601	T2015	T2014	compound	neurotrophin,signaling
R6013	T13917	T13918	advmod	In,situ
R6014	T13918	T13919	amod	situ,hybridization
R6015	T13920	T13919	cc	and,hybridization
R6016	T13921	T13919	conj	immunohistochemistry,hybridization
R6017	T13923	T13924	prep	For,hybridized
R6018	T13925	T13926	advmod	in,situ
R6019	T13926	T13927	amod	situ,analysis
R602	T1934	T1935	compound	Er81,expression
R6020	T13927	T13923	pobj	analysis,For
R6021	T13928	T13927	compound	hybridization,analysis
R6022	T13929	T13924	punct	", ",hybridized
R6023	T13930	T13931	compound	cryostat,sections
R6024	T13931	T13924	nsubjpass	sections,hybridized
R6025	T13932	T13924	auxpass	were,hybridized
R6026	T13933	T13924	advcl	using,hybridized
R6027	T13934	T13935	npadvmod	digoxigenin,labeled
R6028	T13935	T13937	amod	labeled,probes
R6029	T13936	T13935	punct	-,labeled
R603	T2016	T1994	punct	", ",fail
R6030	T13937	T13933	dobj	probes,using
R6031	T13938	T13939	punct	[,45
R6032	T13939	T13937	parataxis	45,probes
R6033	T13940	T13939	punct	],45
R6034	T13941	T13937	acl	directed,probes
R6035	T13942	T13941	prep	against,directed
R6036	T13943	T13944	compound	mouse,TrkA
R6037	T13944	T13942	pobj	TrkA,against
R6038	T13945	T13944	cc	or,TrkA
R6039	T13946	T13944	conj	TrkB,TrkA
R604	T2017	T2018	nmod	group,Ia
R6040	T13947	T13944	punct	", ",TrkA
R6041	T13948	T13944	cc	or,TrkA
R6042	T13949	T13950	compound	rat,TrkC
R6043	T13950	T13944	conj	TrkC,TrkA
R6044	T13951	T13952	punct	(,gift
R6045	T13952	T13950	parataxis	gift,TrkC
R6046	T13953	T13952	prep	from,gift
R6047	T13954	T13955	compound	L.,Parada
R6048	T13955	T13953	pobj	Parada,from
R6049	T13956	T13955	compound	F.,Parada
R605	T2018	T2019	nmod	Ia,afferents
R6050	T13957	T13952	punct	),gift
R6051	T13958	T13924	punct	.,hybridized
R6052	T13960	T13961	nsubj	Antibodies,were
R6053	T13962	T13960	acl	used,Antibodies
R6054	T13963	T13962	prep	in,used
R6055	T13964	T13965	det	this,study
R6056	T13965	T13963	pobj	study,in
R6057	T13966	T13967	mark	as,follows
R6058	T13967	T13961	advcl	follows,were
R6059	T13968	T13961	punct	: ,were
R606	T1935	T1933	pobj	expression,of
R6060	T13969	T13970	compound	rabbit,anti-Er81
R6061	T13970	T13961	dep	anti-Er81,were
R6062	T13971	T13972	punct	[,14
R6063	T13972	T13970	parataxis	14,anti-Er81
R6064	T13973	T13972	punct	],14
R6065	T13974	T13970	punct	", ",anti-Er81
R6066	T13975	T13970	conj	rabbit,anti-Er81
R6067	T13976	T13975	amod	anti-Pea3,rabbit
R6068	T13977	T13978	punct	[,14
R6069	T13978	T13975	parataxis	14,rabbit
R607	T2019	T1994	nsubj	afferents,fail
R6070	T13979	T13978	punct	],14
R6071	T13980	T13975	punct	", ",rabbit
R6072	T13981	T13975	conj	rabbit,rabbit
R6073	T13982	T13981	amod	anti-PV,rabbit
R6074	T13983	T13984	punct	[,14
R6075	T13984	T13981	parataxis	14,rabbit
R6076	T13985	T13984	punct	],14
R6077	T13986	T13981	punct	", ",rabbit
R6078	T13987	T13981	conj	rabbit,rabbit
R6079	T13988	T13987	amod	anti-eGFP,rabbit
R608	T2020	T2019	amod	proprioceptive,afferents
R6080	T13989	T13990	punct	(,Probes
R6081	T13990	T13987	parataxis	Probes,rabbit
R6082	T13991	T13990	compound	Molecular,Probes
R6083	T13992	T13990	punct	", ",Probes
R6084	T13993	T13990	npadvmod	Eugene,Probes
R6085	T13994	T13990	punct	", ",Probes
R6086	T13995	T13990	npadvmod	Oregon,Probes
R6087	T13996	T13990	punct	", ",Probes
R6088	T13997	T13998	compound	United,States
R6089	T13998	T13990	npadvmod	States,Probes
R609	T1936	T1932	prep	in,induction
R6090	T13999	T13990	punct	),Probes
R6091	T14000	T13987	punct	", ",rabbit
R6092	T14001	T13987	conj	rabbit,rabbit
R6093	T14002	T14001	amod	anti-Calbindin,rabbit
R6094	T14003	T14001	punct	", ",rabbit
R6095	T14004	T14001	conj	rabbit,rabbit
R6096	T14005	T14004	amod	anti-Calretinin,rabbit
R6097	T14006	T14007	punct	(,Swant
R6098	T14007	T14004	parataxis	Swant,rabbit
R6099	T14008	T14007	punct	", ",Swant
R610	T2021	T2022	aux	to,invade
R6100	T14009	T14007	npadvmod	Bellinzona,Swant
R6101	T14010	T14007	punct	", ",Swant
R6102	T14011	T14007	npadvmod	Switzerland,Swant
R6103	T14012	T14007	punct	),Swant
R6104	T14013	T14004	punct	", ",rabbit
R6105	T14014	T14004	conj	rabbit,rabbit
R6106	T14015	T14014	amod	anti-CGRP,rabbit
R6107	T14016	T14017	punct	(,Chemicon
R6108	T14017	T14014	parataxis	Chemicon,rabbit
R6109	T14018	T14017	punct	", ",Chemicon
R611	T2022	T1994	xcomp	invade,fail
R6110	T14019	T14017	npadvmod	Temecula,Chemicon
R6111	T14020	T14017	punct	", ",Chemicon
R6112	T14021	T14017	npadvmod	California,Chemicon
R6113	T14022	T14017	punct	", ",Chemicon
R6114	T14023	T14024	compound	United,States
R6115	T14024	T14017	npadvmod	States,Chemicon
R6116	T14025	T14017	punct	),Chemicon
R6117	T14026	T14014	punct	", ",rabbit
R6118	T14027	T14014	conj	rabbit,rabbit
R6119	T14028	T14027	amod	anti-vGlut1,rabbit
R612	T2023	T2024	det	the,cord
R6120	T14029	T14030	punct	(,Systems
R6121	T14030	T14027	parataxis	Systems,rabbit
R6122	T14031	T14030	compound	Synaptic,Systems
R6123	T14032	T14030	punct	", ",Systems
R6124	T14033	T14030	npadvmod	Goettingen,Systems
R6125	T14034	T14030	punct	", ",Systems
R6126	T14035	T14030	npadvmod	Germany,Systems
R6127	T14036	T14030	punct	),Systems
R6128	T14037	T14027	punct	", ",rabbit
R6129	T14038	T14027	conj	rabbit,rabbit
R613	T1937	T1938	amod	proprioceptive,afferents
R6130	T14039	T14038	amod	anti-Brn3a,rabbit
R6131	T14040	T14041	punct	(,gift
R6132	T14041	T14038	parataxis	gift,rabbit
R6133	T14042	T14041	prep	from,gift
R6134	T14043	T14044	compound	E.,Turner
R6135	T14044	T14042	pobj	Turner,from
R6136	T14045	T14041	punct	),gift
R6137	T14046	T14038	punct	", ",rabbit
R6138	T14047	T14048	nmod	rabbit,p75
R6139	T14048	T14038	conj	p75,rabbit
R614	T2024	T2022	dobj	cord,invade
R6140	T14049	T14048	amod	anti-TrkA,p75
R6141	T14050	T14048	cc	and,p75
R6142	T14051	T14048	punct	-,p75
R6143	T14052	T14053	punct	(,gift
R6144	T14053	T14048	parataxis	gift,p75
R6145	T14054	T14053	prep	from,gift
R6146	T14055	T14056	compound	L.,Reichardt
R6147	T14056	T14054	pobj	Reichardt,from
R6148	T14057	T14056	compound	F.,Reichardt
R6149	T14058	T14053	punct	),gift
R615	T2025	T2024	amod	ventral,cord
R6150	T14059	T14048	punct	", ",p75
R6151	T14060	T14048	conj	rabbit,p75
R6152	T14061	T14060	amod	anti-Runx3,rabbit
R6153	T14062	T14063	punct	(,Kramer
R6154	T14063	T14060	parataxis	Kramer,rabbit
R6155	T14064	T14063	cc	and,Kramer
R6156	T14065	T14063	conj	Arber,Kramer
R6157	T14066	T14063	punct	", ",Kramer
R6158	T14067	T14068	amod	unpublished,reagent
R6159	T14068	T14063	npadvmod	reagent,Kramer
R616	T1938	T1936	pobj	afferents,in
R6160	T14069	T14063	punct	),Kramer
R6161	T14070	T14060	punct	", ",rabbit
R6162	T14071	T14060	conj	rabbit,rabbit
R6163	T14072	T14071	amod	anti-Rhodamine,rabbit
R6164	T14073	T14074	punct	(,Probes
R6165	T14074	T14071	parataxis	Probes,rabbit
R6166	T14075	T14074	compound	Molecular,Probes
R6167	T14076	T14074	punct	),Probes
R6168	T14077	T14071	punct	", ",rabbit
R6169	T14078	T14071	conj	mouse,rabbit
R617	T2026	T2024	amod	spinal,cord
R6170	T14079	T14078	amod	anti-neurofilament,mouse
R6171	T14080	T14081	punct	(,Collection
R6172	T14081	T14078	parataxis	Collection,mouse
R6173	T14082	T14081	compound	American,Collection
R6174	T14083	T14081	compound	Type,Collection
R6175	T14084	T14081	compound	Culture,Collection
R6176	T14085	T14081	punct	", ",Collection
R6177	T14086	T14081	npadvmod	Manassas,Collection
R6178	T14087	T14081	punct	", ",Collection
R6179	T14088	T14081	npadvmod	Virginia,Collection
R618	T2027	T2022	cc	and,invade
R6180	T14089	T14081	punct	", ",Collection
R6181	T14090	T14091	compound	United,States
R6182	T14091	T14081	npadvmod	States,Collection
R6183	T14092	T14081	punct	),Collection
R6184	T14093	T14078	punct	", ",mouse
R6185	T14094	T14078	conj	sheep,mouse
R6186	T14095	T14094	amod	anti-eGFP,sheep
R6187	T14096	T14097	punct	(,Biogenesis
R6188	T14097	T14094	parataxis	Biogenesis,sheep
R6189	T14098	T14097	punct	", ",Biogenesis
R619	T2028	T2029	aux	to,make
R6190	T14099	T14097	npadvmod	Poole,Biogenesis
R6191	T14100	T14097	punct	", ",Biogenesis
R6192	T14101	T14102	compound	United,Kingdom
R6193	T14102	T14097	npadvmod	Kingdom,Biogenesis
R6194	T14103	T14097	punct	),Biogenesis
R6195	T14104	T14094	punct	", ",sheep
R6196	T14105	T14094	conj	goat,sheep
R6197	T14106	T14105	amod	anti-LacZ,goat
R6198	T14107	T14108	punct	[,14
R6199	T14108	T14105	parataxis	14,goat
R620	T2029	T2022	conj	make,invade
R6200	T14109	T14108	punct	],14
R6201	T14110	T14105	punct	", ",goat
R6202	T14111	T14105	conj	goat,goat
R6203	T14112	T14111	amod	anti-TrkC,goat
R6204	T14113	T14114	punct	(,gift
R6205	T14114	T14111	parataxis	gift,goat
R6206	T14115	T14114	prep	from,gift
R6207	T14116	T14117	compound	L.,Reichardt
R6208	T14117	T14115	pobj	Reichardt,from
R6209	T14118	T14117	compound	F.,Reichardt
R621	T1939	T1929	auxpass	is,known
R6210	T14119	T14114	punct	),gift
R6211	T14120	T14111	punct	", ",goat
R6212	T14121	T14111	cc	and,goat
R6213	T14122	T14123	compound	guinea,pig
R6214	T14123	T14111	conj	pig,goat
R6215	T14124	T14123	amod	anti-Isl1,pig
R6216	T14125	T14126	punct	[,14
R6217	T14126	T14123	parataxis	14,pig
R6218	T14127	T14126	punct	],14
R6219	T14128	T13961	punct	.,were
R622	T1940	T1941	aux	to,mediated
R6220	T14130	T14131	amod	Terminal,transferase
R6221	T14131	T14133	npadvmod	transferase,mediated
R6222	T14132	T14131	compound	deoxynucleotidyl,transferase
R6223	T14133	T14135	amod	mediated,labeling
R6224	T14134	T14133	punct	-,mediated
R6225	T14135	T14140	nsubjpass	labeling,performed
R6226	T14136	T14135	amod	biotinylated,labeling
R6227	T14137	T14135	compound	UTP,labeling
R6228	T14138	T14135	compound	nick,labeling
R6229	T14139	T14135	compound	end,labeling
R623	T2030	T2031	amod	effective,connections
R6230	T14141	T14135	punct	(,labeling
R6231	T14142	T14135	appos	TUNEL,labeling
R6232	T14143	T14135	punct	),labeling
R6233	T14144	T14145	aux	to,detect
R6234	T14145	T14135	advcl	detect,labeling
R6235	T14146	T14147	amod	apoptotic,cells
R6236	T14147	T14145	dobj	cells,detect
R6237	T14148	T14145	prep	in,detect
R6238	T14149	T14150	compound	E13.5,DRG
R6239	T14150	T14148	pobj	DRG,in
R624	T1941	T1929	xcomp	mediated,known
R6240	T14151	T14145	prep	on,detect
R6241	T14152	T14153	compound	cryostat,sections
R6242	T14153	T14151	pobj	sections,on
R6243	T14154	T14140	auxpass	was,performed
R6244	T14155	T14156	mark	as,described
R6245	T14156	T14140	advcl	described,performed
R6246	T14157	T14156	agent	by,described
R6247	T14158	T14159	det	the,manufacturer
R6248	T14159	T14157	pobj	manufacturer,by
R6249	T14160	T14161	punct	(,Roche
R625	T2031	T2029	dobj	connections,make
R6250	T14161	T14159	parataxis	Roche,manufacturer
R6251	T14162	T14161	punct	", ",Roche
R6252	T14163	T14161	npadvmod	Basel,Roche
R6253	T14164	T14161	punct	", ",Roche
R6254	T14165	T14161	npadvmod	Switzerland,Roche
R6255	T14166	T14161	punct	),Roche
R6256	T14167	T14140	punct	.,performed
R6257	T14169	T14170	amod	Quantitative,analysis
R6258	T14170	T14171	nsubjpass	analysis,performed
R6259	T14172	T14170	prep	of,analysis
R626	T2032	T2031	amod	synaptic,connections
R6260	T14173	T14174	nmod	TUNEL,cells
R6261	T14174	T14172	pobj	cells,of
R6262	T14175	T14173	punct	+,TUNEL
R6263	T14176	T14174	compound	DRG,cells
R6264	T14177	T14171	auxpass	was,performed
R6265	T14178	T14179	advmod	essentially,described
R6266	T14179	T14171	advcl	described,performed
R6267	T14180	T14179	mark	as,described
R6268	T14181	T14182	punct	[,27
R6269	T14182	T14179	parataxis	27,described
R627	T2033	T2031	prep	with,connections
R6270	T14183	T14182	punct	],27
R6271	T14184	T14171	punct	.,performed
R6272	T14186	T14187	compound	BrdU,experiments
R6273	T14187	T14191	nsubjpass	experiments,performed
R6274	T14188	T14189	compound	pulse,chase
R6275	T14189	T14187	compound	chase,experiments
R6276	T14190	T14189	punct	-,chase
R6277	T14192	T14187	cc	and,experiments
R6278	T14193	T14194	compound	LacZ,stainings
R6279	T14194	T14187	conj	stainings,experiments
R628	T1942	T1941	auxpass	be,mediated
R6280	T14195	T14194	compound	wholemount,stainings
R6281	T14196	T14191	auxpass	were,performed
R6282	T14197	T14198	mark	as,described
R6283	T14198	T14191	advcl	described,performed
R6284	T14199	T14198	advmod	previously,described
R6285	T14200	T14201	punct	[,46
R6286	T14201	T14198	parataxis	46,described
R6287	T14202	T14201	punct	],46
R6288	T14203	T14191	punct	.,performed
R6289	T14205	T14206	mark	For,visualize
R629	T2034	T2035	compound	motor,neurons
R6290	T14206	T14211	advcl	visualize,injected
R6291	T14207	T14208	amod	anterograde,experiments
R6292	T14208	T14206	nsubj	experiments,visualize
R6293	T14209	T14208	compound	tracing,experiments
R6294	T14210	T14206	aux	to,visualize
R6295	T14212	T14206	dobj	projections,visualize
R6296	T14213	T14212	prep	of,projections
R6297	T14214	T14215	amod	sensory,neurons
R6298	T14215	T14213	pobj	neurons,of
R6299	T14216	T14211	punct	", ",injected
R630	T2035	T2033	pobj	neurons,with
R6300	T14217	T14218	npadvmod	rhodamine,conjugated
R6301	T14218	T14220	amod	conjugated,dextran
R6302	T14219	T14218	punct	-,conjugated
R6303	T14220	T14211	nsubjpass	dextran,injected
R6304	T14221	T14222	punct	(,Probes
R6305	T14222	T14220	parataxis	Probes,dextran
R6306	T14223	T14222	compound	Molecular,Probes
R6307	T14224	T14222	punct	),Probes
R6308	T14225	T14211	auxpass	was,injected
R6309	T14226	T14211	prep	into,injected
R631	T2036	T2037	punct	[,14
R6310	T14227	T14228	amod	single,DRG
R6311	T14228	T14226	pobj	DRG,into
R6312	T14229	T14228	nmod	lumbar,DRG
R6313	T14230	T14231	punct	(,L3
R6314	T14231	T14228	parataxis	L3,DRG
R6315	T14232	T14231	punct	),L3
R6316	T14233	T14211	prep	at,injected
R6317	T14234	T14233	pobj	E13.5,at
R6318	T14235	T14211	cc	or,injected
R6319	T14236	T14211	conj	applied,injected
R632	T1943	T1941	agent	by,mediated
R6320	T14237	T14236	prep	to,applied
R6321	T14238	T14239	amod	whole,roots
R6322	T14239	T14237	pobj	roots,to
R6323	T14240	T14239	amod	lumbar,roots
R6324	T14241	T14239	amod	dorsal,roots
R6325	T14242	T14243	punct	(,L3
R6326	T14243	T14239	parataxis	L3,roots
R6327	T14244	T14243	punct	),L3
R6328	T14245	T14236	prep	at,applied
R6329	T14246	T14247	amod	postnatal,day
R633	T2037	T1994	parataxis	14,fail
R6330	T14247	T14245	pobj	day,at
R6331	T14248	T14247	punct	(,day
R6332	T14249	T14247	appos	P,day
R6333	T14250	T14247	punct	),day
R6334	T14251	T14247	nummod	5,day
R6335	T14252	T14236	advcl	using,applied
R6336	T14253	T14254	compound	glass,capillaries
R6337	T14254	T14252	dobj	capillaries,using
R6338	T14255	T14211	punct	.,injected
R6339	T14257	T14258	prep	After,incubated
R634	T2038	T2037	nummod	9,14
R6340	T14259	T14257	pobj	injection,After
R6341	T14260	T14258	punct	", ",incubated
R6342	T14261	T14258	nsubjpass	animals,incubated
R6343	T14262	T14258	auxpass	were,incubated
R6344	T14263	T14258	prep	for,incubated
R6345	T14264	T14265	quantmod	2,3
R6346	T14265	T14267	nummod	3,h
R6347	T14266	T14265	punct	–,3
R6348	T14267	T14263	pobj	h,for
R6349	T14268	T14269	punct	(,E13.5
R635	T1944	T1945	amod	peripheral,neurotrophin
R6350	T14269	T14267	parataxis	E13.5,h
R6351	T14270	T14269	punct	),E13.5
R6352	T14271	T14263	cc	or,for
R6353	T14272	T14263	conj	overnight,for
R6354	T14273	T14274	punct	(,P5
R6355	T14274	T14272	parataxis	P5,overnight
R6356	T14275	T14274	punct	),P5
R6357	T14276	T14258	punct	.,incubated
R6358	T14278	T14279	compound	Cryostat,sections
R6359	T14279	T14280	nsubjpass	sections,processed
R636	T2039	T2037	punct	",",14
R6360	T14281	T14280	auxpass	were,processed
R6361	T14282	T14280	prep	for,processed
R6362	T14283	T14282	pobj	immunohistochemistry,for
R6363	T14284	T14285	mark	as,described
R6364	T14285	T14280	advcl	described,processed
R6365	T14286	T14287	punct	[,14
R6366	T14287	T14285	parataxis	14,described
R6367	T14288	T14287	punct	],14
R6368	T14289	T14280	advcl	using,processed
R6369	T14290	T14291	npadvmod	fluorophore,conjugated
R637	T2040	T2037	punct	],14
R6370	T14291	T14293	amod	conjugated,antibodies
R6371	T14292	T14291	punct	-,conjugated
R6372	T14293	T14289	dobj	antibodies,using
R6373	T14294	T14293	amod	secondary,antibodies
R6374	T14295	T14296	punct	(,Probes
R6375	T14296	T14293	parataxis	Probes,antibodies
R6376	T14297	T14296	dep	1,Probes
R6377	T14298	T14299	punct	:,"1,000"
R6378	T14299	T14297	prep	"1,000",1
R6379	T14300	T14296	punct	", ",Probes
R638	T1945	T1943	pobj	neurotrophin,by
R6380	T14301	T14296	compound	Molecular,Probes
R6381	T14302	T14296	punct	),Probes
R6382	T14303	T14280	punct	.,processed
R6383	T14305	T14306	nsubjpass	Images,collected
R6384	T14307	T14306	auxpass	were,collected
R6385	T14308	T14306	prep	on,collected
R6386	T14309	T14310	det	an,microscope
R6387	T14310	T14308	pobj	microscope,on
R6388	T14311	T14310	nmod	Olympus,microscope
R6389	T14312	T14313	punct	(,Tokyo
R639	T2041	T1994	punct	.,fail
R6390	T14313	T14311	parataxis	Tokyo,Olympus
R6391	T14314	T14313	punct	", ",Tokyo
R6392	T14315	T14313	npadvmod	Japan,Tokyo
R6393	T14316	T14313	punct	),Tokyo
R6394	T14317	T14310	amod	confocal,microscope
R6395	T14318	T14306	punct	.,collected
R6396	T14320	T14321	nsubjpass	Images,collected
R6397	T14322	T14320	prep	from,Images
R6398	T14323	T14324	nmod	in,situ
R6399	T14324	T14325	nmod	situ,experiments
R640	T1946	T1945	nummod	3,neurotrophin
R6400	T14325	T14322	pobj	experiments,from
R6401	T14326	T14325	compound	hybridization,experiments
R6402	T14327	T14321	auxpass	were,collected
R6403	T14328	T14321	prep	with,collected
R6404	T14329	T14330	det	an,camera
R6405	T14330	T14328	pobj	camera,with
R6406	T14331	T14332	compound	RT,SPOT
R6407	T14332	T14330	compound	SPOT,camera
R6408	T14333	T14332	punct	-,SPOT
R6409	T14334	T14335	punct	(,Instruments
R641	T2043	T2044	det	The,involvement
R6410	T14335	T14330	parataxis	Instruments,camera
R6411	T14336	T14335	amod	Diagnostic,Instruments
R6412	T14337	T14335	punct	", ",Instruments
R6413	T14338	T14339	compound	Sterling,Heights
R6414	T14339	T14335	npadvmod	Heights,Instruments
R6415	T14340	T14335	punct	", ",Instruments
R6416	T14341	T14335	npadvmod	Michigan,Instruments
R6417	T14342	T14335	punct	", ",Instruments
R6418	T14343	T14344	compound	United,States
R6419	T14344	T14335	npadvmod	States,Instruments
R642	T1947	T1945	punct	(,neurotrophin
R6420	T14345	T14335	punct	),Instruments
R6421	T14346	T14321	punct	", ",collected
R6422	T14347	T14321	cc	and,collected
R6423	T14348	T14349	nmod	Corel,Paint
R6424	T14349	T14360	nsubjpass	Paint,used
R6425	T14350	T14351	punct	(,Prairie
R6426	T14351	T14348	parataxis	Prairie,Corel
R6427	T14352	T14351	compound	Eden,Prairie
R6428	T14353	T14351	punct	", ",Prairie
R6429	T14354	T14351	npadvmod	Minnesota,Prairie
R643	T2044	T2045	nsubj	involvement,raises
R6430	T14355	T14351	punct	", ",Prairie
R6431	T14356	T14357	compound	United,States
R6432	T14357	T14351	npadvmod	States,Prairie
R6433	T14358	T14351	punct	),Prairie
R6434	T14359	T14349	compound	Photo,Paint
R6435	T14360	T14321	conj	used,collected
R6436	T14361	T14349	nummod	10.0,Paint
R6437	T14362	T14360	auxpass	was,used
R6438	T14363	T14360	prep	for,used
R6439	T14364	T14365	amod	digital,processing
R644	T1948	T1945	appos	NT,neurotrophin
R6440	T14365	T14363	pobj	processing,for
R6441	T14366	T14365	prep	of,processing
R6442	T14367	T14366	pobj	images,of
R6443	T14368	T14360	punct	.,used
R6444	T14469	T14470	advmod	In,vitro
R6445	T14470	T14471	amod	vitro,cultures
R6446	T14472	T14471	prep	of,cultures
R6447	T14473	T14472	pobj	DRG,of
R6448	T14475	T14476	amod	Individual,DRG
R6449	T14476	T14478	nsubjpass	DRG,dissected
R645	T2046	T2044	prep	of,involvement
R6450	T14477	T14476	compound	lumbar,DRG
R6451	T14479	T14478	auxpass	were,dissected
R6452	T14480	T14478	prep	from,dissected
R6453	T14481	T14482	nmod	E13.5,embryos
R6454	T14482	T14480	pobj	embryos,from
R6455	T14483	T14481	cc	or,E13.5
R6456	T14484	T14481	conj	E14.5,E13.5
R6457	T14485	T14478	cc	and,dissected
R6458	T14486	T14478	conj	placed,dissected
R6459	T14487	T14486	prep	on,placed
R646	T2047	T2048	npadvmod	target,derived
R6460	T14488	T14489	npadvmod	Matrigel,coated
R6461	T14489	T14502	amod	coated,coverslips
R6462	T14490	T14491	punct	(,Biosciences
R6463	T14491	T14488	parataxis	Biosciences,Matrigel
R6464	T14492	T14491	compound	BD,Biosciences
R6465	T14493	T14491	punct	", ",Biosciences
R6466	T14494	T14495	compound	San,Jose
R6467	T14495	T14491	npadvmod	Jose,Biosciences
R6468	T14496	T14491	punct	", ",Biosciences
R6469	T14497	T14491	npadvmod	California,Biosciences
R647	T2048	T2050	amod	derived,signals
R6470	T14498	T14491	punct	", ",Biosciences
R6471	T14499	T14500	compound	United,States
R6472	T14500	T14491	npadvmod	States,Biosciences
R6473	T14501	T14491	punct	),Biosciences
R6474	T14502	T14487	pobj	coverslips,on
R6475	T14503	T14502	prep	in,coverslips
R6476	T14504	T14505	compound	DMEM,F12
R6477	T14505	T14503	pobj	F12,in
R6478	T14506	T14505	punct	/,F12
R6479	T14507	T14508	punct	(,Gibco
R648	T1949	T1948	punct	-,NT
R6480	T14508	T14505	parataxis	Gibco,F12
R6481	T14509	T14508	punct	", ",Gibco
R6482	T14510	T14511	compound	San,Diego
R6483	T14511	T14508	npadvmod	Diego,Gibco
R6484	T14512	T14508	punct	", ",Gibco
R6485	T14513	T14508	npadvmod	California,Gibco
R6486	T14514	T14508	punct	", ",Gibco
R6487	T14515	T14516	compound	United,States
R6488	T14516	T14508	npadvmod	States,Gibco
R6489	T14517	T14508	punct	),Gibco
R649	T2049	T2048	punct	-,derived
R6490	T14518	T14502	punct	", ",coverslips
R6491	T14519	T14520	nummod	2,mM
R6492	T14520	T14521	compound	mM,Gln
R6493	T14521	T14502	conj	Gln,coverslips
R6494	T14522	T14521	compound	L,Gln
R6495	T14523	T14521	punct	-,Gln
R6496	T14524	T14525	punct	(,Gibco
R6497	T14525	T14521	parataxis	Gibco,Gln
R6498	T14526	T14525	punct	),Gibco
R6499	T14527	T14521	punct	", ",Gln
R650	T2050	T2046	pobj	signals,of
R6500	T14528	T14521	conj	N2,Gln
R6501	T14529	T14530	punct	(,Gibco
R6502	T14530	T14528	parataxis	Gibco,N2
R6503	T14531	T14530	punct	),Gibco
R6504	T14532	T14528	punct	", ",N2
R6505	T14533	T14528	cc	and,N2
R6506	T14534	T14535	nummod	1,mg
R6507	T14535	T14536	nmod	mg,BSA
R6508	T14536	T14528	conj	BSA,N2
R6509	T14537	T14538	punct	/,ml
R651	T1950	T1948	nummod	3,NT
R6510	T14538	T14535	prep	ml,mg
R6511	T14539	T14540	punct	(,Sigma
R6512	T14540	T14536	parataxis	Sigma,BSA
R6513	T14541	T14540	punct	", ",Sigma
R6514	T14542	T14543	compound	St.,Louis
R6515	T14543	T14540	npadvmod	Louis,Sigma
R6516	T14544	T14540	punct	", ",Sigma
R6517	T14545	T14540	npadvmod	Missouri,Sigma
R6518	T14546	T14540	punct	", ",Sigma
R6519	T14547	T14548	compound	United,States
R652	T2051	T2044	prep	in,involvement
R6520	T14548	T14540	npadvmod	States,Sigma
R6521	T14549	T14540	punct	),Sigma
R6522	T14550	T14536	prep	without,BSA
R6523	T14551	T14550	pobj	neurotrophins,without
R6524	T14552	T14478	punct	", ",dissected
R6525	T14553	T14478	cc	or,dissected
R6526	T14554	T14478	conj	supplemented,dissected
R6527	T14555	T14554	prep	with,supplemented
R6528	T14556	T14557	preconj	either,NGF
R6529	T14557	T14555	pobj	NGF,with
R653	T2052	T2051	pobj	induction,in
R6530	T14558	T14559	punct	(,Gibco
R6531	T14559	T14557	parataxis	Gibco,NGF
R6532	T14560	T14561	nummod	100,ng
R6533	T14561	T14559	dep	ng,Gibco
R6534	T14562	T14563	punct	/,ml
R6535	T14563	T14561	prep	ml,ng
R6536	T14564	T14559	punct	", ",Gibco
R6537	T14565	T14559	punct	),Gibco
R6538	T14566	T14557	cc	or,NGF
R6539	T14567	T14557	conj	NT,NGF
R654	T2053	T2052	prep	of,induction
R6540	T14568	T14567	punct	-,NT
R6541	T14569	T14567	nummod	3,NT
R6542	T14570	T14571	punct	(,Sigma
R6543	T14571	T14567	parataxis	Sigma,NT
R6544	T14572	T14573	nummod	20,ng
R6545	T14573	T14571	dep	ng,Sigma
R6546	T14574	T14575	punct	/,ml
R6547	T14575	T14573	prep	ml,ng
R6548	T14576	T14571	punct	", ",Sigma
R6549	T14577	T14571	punct	),Sigma
R655	T1951	T1941	punct	),mediated
R6550	T14578	T14478	punct	.,dissected
R6551	T14580	T14581	compound	DRG,explants
R6552	T14581	T14582	nsubjpass	explants,cultured
R6553	T14583	T14584	punct	(,20
R6554	T14584	T14581	parataxis	20,explants
R6555	T14585	T14584	nsubj	n,20
R6556	T14586	T14584	punct	≥,20
R6557	T14587	T14584	prep	for,20
R6558	T14588	T14589	det	each,condition
R6559	T14589	T14587	pobj	condition,for
R656	T2054	T2055	compound	ETS,expression
R6560	T14590	T14584	punct	),20
R6561	T14591	T14582	auxpass	were,cultured
R6562	T14592	T14582	prep	for,cultured
R6563	T14593	T14594	nummod	48,h
R6564	T14594	T14592	pobj	h,for
R6565	T14595	T14582	punct	", ",cultured
R6566	T14596	T14582	conj	processed,cultured
R6567	T14597	T14596	prep	for,processed
R6568	T14598	T14597	pobj	immunocytochemistry,for
R6569	T14599	T14596	punct	", ",processed
R657	T2055	T2053	pobj	expression,of
R6570	T14600	T14596	cc	and,processed
R6571	T14601	T14596	conj	analyzed,processed
R6572	T14602	T14601	advcl	using,analyzed
R6573	T14603	T14604	amod	confocal,microscopy
R6574	T14604	T14602	dobj	microscopy,using
R6575	T14605	T14582	punct	.,cultured
R6577	T14754	T14755	compound	Western,blot
R6578	T14755	T14756	compound	blot,analysis
R6579	T14758	T14759	compound	Lumbar,DRG
R658	T1952	T1953	punct	[,9
R6580	T14759	T14760	nsubjpass	DRG,isolated
R6581	T14761	T14759	prep	from,DRG
R6582	T14762	T14763	compound	E16.5,embryos
R6583	T14763	T14761	pobj	embryos,from
R6584	T14764	T14760	auxpass	were,isolated
R6585	T14765	T14760	punct	", ",isolated
R6586	T14766	T14767	advmod	mechanically,disrupted
R6587	T14767	T14760	conj	disrupted,isolated
R6588	T14768	T14767	punct	", ",disrupted
R6589	T14769	T14767	conj	homogenized,disrupted
R659	T2056	T2055	compound	transcription,expression
R6590	T14770	T14769	advcl	using,homogenized
R6591	T14771	T14772	compound	glass,beads
R6592	T14772	T14770	dobj	beads,using
R6593	T14773	T14774	punct	(,Sigma
R6594	T14774	T14772	parataxis	Sigma,beads
R6595	T14775	T14774	punct	),Sigma
R6596	T14776	T14769	punct	", ",homogenized
R6597	T14777	T14769	cc	and,homogenized
R6598	T14778	T14769	conj	lysed,homogenized
R6599	T14779	T14778	prep	in,lysed
R660	T1953	T1941	parataxis	9,mediated
R6600	T14780	T14781	amod	standard,buffer
R6601	T14781	T14779	pobj	buffer,in
R6602	T14782	T14781	compound	lysis,buffer
R6603	T14783	T14781	acl	supplemented,buffer
R6604	T14784	T14783	prep	with,supplemented
R6605	T14785	T14786	nmod	protease,inhibitors
R6606	T14786	T14784	pobj	inhibitors,with
R6607	T14787	T14785	cc	and,protease
R6608	T14788	T14785	conj	phosphatase,protease
R6609	T14789	T14790	mark	as,described
R661	T2057	T2055	compound	factor,expression
R6610	T14790	T14783	advcl	described,supplemented
R6611	T14791	T14792	punct	[,47
R6612	T14792	T14790	parataxis	47,described
R6613	T14793	T14792	punct	],47
R6614	T14794	T14760	punct	.,isolated
R6615	T14796	T14797	compound	Protein,extracts
R6616	T14797	T14798	nsubjpass	extracts,resolved
R6617	T14799	T14798	auxpass	were,resolved
R6618	T14800	T14798	agent	by,resolved
R6619	T14801	T14802	compound	SDS,PAGE
R662	T2058	T2059	det	the,question
R6620	T14802	T14800	pobj	PAGE,by
R6621	T14803	T14802	punct	-,PAGE
R6622	T14804	T14798	punct	", ",resolved
R6623	T14805	T14798	cc	and,resolved
R6624	T14806	T14807	nsubjpass	immunoblotting,performed
R6625	T14807	T14798	conj	performed,resolved
R6626	T14808	T14807	auxpass	was,performed
R6627	T14809	T14807	advcl	using,performed
R6628	T14810	T14809	dobj	antibodies,using
R6629	T14811	T14810	prep	against,antibodies
R663	T1954	T1953	punct	],9
R6630	T14812	T14811	pobj	Akt,against
R6631	T14813	T14812	punct	", ",Akt
R6632	T14814	T14815	compound	p,Akt
R6633	T14815	T14812	conj	Akt,Akt
R6634	T14816	T14815	punct	-,Akt
R6635	T14817	T14818	punct	(,Ser473
R6636	T14818	T14815	parataxis	Ser473,Akt
R6637	T14819	T14818	punct	),Ser473
R6638	T14820	T14815	punct	", ",Akt
R6639	T14821	T14815	conj	CREB,Akt
R664	T2059	T2045	dobj	question,raises
R6640	T14822	T14821	punct	", ",CREB
R6641	T14823	T14824	compound	p,CREB
R6642	T14824	T14821	conj	CREB,CREB
R6643	T14825	T14824	punct	-,CREB
R6644	T14826	T14827	punct	(,Ser133
R6645	T14827	T14824	parataxis	Ser133,CREB
R6646	T14828	T14827	punct	),Ser133
R6647	T14829	T14824	punct	", ",CREB
R6648	T14830	T14824	conj	Bax,CREB
R6649	T14831	T14830	punct	", ",Bax
R665	T1955	T1929	punct	.,known
R6650	T14832	T14833	compound	Bcl,xl
R6651	T14833	T14830	conj	xl,Bax
R6652	T14834	T14833	punct	-,xl
R6653	T14835	T14836	punct	(,Technology
R6654	T14836	T14833	parataxis	Technology,xl
R6655	T14837	T14836	compound	Cell,Technology
R6656	T14838	T14836	compound	Signaling,Technology
R6657	T14839	T14836	punct	", ",Technology
R6658	T14840	T14836	npadvmod	Beverly,Technology
R6659	T14841	T14836	punct	", ",Technology
R666	T2060	T2059	prep	of,question
R6660	T14842	T14836	npadvmod	Massachusetts,Technology
R6661	T14843	T14836	punct	", ",Technology
R6662	T14844	T14845	compound	United,States
R6663	T14845	T14836	npadvmod	States,Technology
R6664	T14846	T14836	punct	),Technology
R6665	T14847	T14833	punct	", ",xl
R6666	T14848	T14833	cc	and,xl
R6667	T14849	T14833	conj	Bcl2,xl
R6668	T14850	T14851	punct	(,Pharmingen
R6669	T14851	T14849	parataxis	Pharmingen,Bcl2
R667	T2061	T2062	det	the,necessity
R6670	T14852	T14851	compound	BD,Pharmingen
R6671	T14853	T14851	punct	", ",Pharmingen
R6672	T14854	T14855	compound	San,Diego
R6673	T14855	T14851	npadvmod	Diego,Pharmingen
R6674	T14856	T14851	punct	", ",Pharmingen
R6675	T14857	T14851	npadvmod	California,Pharmingen
R6676	T14858	T14851	punct	", ",Pharmingen
R6677	T14859	T14860	compound	United,States
R6678	T14860	T14851	npadvmod	States,Pharmingen
R6679	T14861	T14851	punct	),Pharmingen
R668	T2062	T2060	pobj	necessity,of
R6680	T14862	T14807	punct	.,performed
R6681	T14864	T14865	prep	For,scanned
R6682	T14866	T14864	pobj	quantification,For
R6683	T14867	T14865	punct	", ",scanned
R6684	T14868	T14865	nsubjpass	films,scanned
R6685	T14869	T14870	punct	(,AR
R6686	T14870	T14868	parataxis	AR,films
R6687	T14871	T14872	compound	X,OMAT
R6688	T14872	T14870	compound	OMAT,AR
R6689	T14873	T14872	punct	-,OMAT
R669	T2063	T2062	prep	for,necessity
R6690	T14874	T14870	punct	", ",AR
R6691	T14875	T14876	compound	Eastman,Kodak
R6692	T14876	T14870	npadvmod	Kodak,AR
R6693	T14877	T14870	punct	", ",AR
R6694	T14878	T14870	npadvmod	Rochester,AR
R6695	T14879	T14870	punct	", ",AR
R6696	T14880	T14881	compound	New,York
R6697	T14881	T14870	npadvmod	York,AR
R6698	T14882	T14870	punct	", ",AR
R6699	T14883	T14884	compound	United,States
R670	T2064	T2065	det	the,delay
R6700	T14884	T14870	npadvmod	States,AR
R6701	T14885	T14870	punct	),AR
R6702	T14886	T14865	auxpass	were,scanned
R6703	T14887	T14865	cc	and,scanned
R6704	T14888	T14889	nsubjpass	densitometry,performed
R6705	T14889	T14865	conj	performed,scanned
R6706	T14890	T14889	auxpass	was,performed
R6707	T14891	T14889	advcl	using,performed
R6708	T14892	T14891	dobj	IMAGEQUANT,using
R6709	T14893	T14892	nummod	5.2,IMAGEQUANT
R671	T2065	T2063	pobj	delay,for
R6710	T14894	T14895	punct	(,Dynamics
R6711	T14895	T14892	parataxis	Dynamics,IMAGEQUANT
R6712	T14896	T14895	compound	Molecular,Dynamics
R6713	T14897	T14895	punct	", ",Dynamics
R6714	T14898	T14895	npadvmod	Amersham,Dynamics
R6715	T14899	T14895	punct	", ",Dynamics
R6716	T14900	T14895	npadvmod	Uppsala,Dynamics
R6717	T14901	T14895	punct	", ",Dynamics
R6718	T14902	T14895	npadvmod	Sweden,Dynamics
R6719	T14903	T14895	punct	),Dynamics
R672	T1957	T1958	det	These,proteins
R6720	T14904	T14889	punct	.,performed
R6721	T15022	T15023	amod	Electrophysiological,analysis
R6722	T15023	T15024	nsubjpass	analysis,performed
R6723	T15025	T15024	auxpass	was,performed
R6724	T15026	T15027	mark	as,described
R6725	T15027	T15024	advcl	described,performed
R6726	T15028	T15027	advmod	previously,described
R6727	T15029	T15030	punct	[,48
R6728	T15030	T15027	parataxis	48,described
R6729	T15031	T15030	punct	],48
R673	T2066	T2065	amod	observed,delay
R6730	T15032	T15024	punct	.,performed
R6731	T15034	T15035	advmod	Briefly,made
R6732	T15036	T15035	punct	", ",made
R6733	T15037	T15038	amod	intracellular,recordings
R6734	T15038	T15035	nsubjpass	recordings,made
R6735	T15039	T15038	prep	from,recordings
R6736	T15040	T15041	amod	identified,neurons
R6737	T15041	T15039	pobj	neurons,from
R6738	T15042	T15041	compound	quadriceps,neurons
R6739	T15043	T15041	compound	motor,neurons
R674	T2067	T2065	prep	in,delay
R6740	T15044	T15035	auxpass	were,made
R6741	T15045	T15035	advcl	using,made
R6742	T15046	T15047	amod	sharp,electrodes
R6743	T15047	T15045	dobj	electrodes,using
R6744	T15048	T15049	punct	(,MΩ
R6745	T15049	T15045	parataxis	MΩ,using
R6746	T15050	T15051	quantmod	75,120
R6747	T15051	T15049	nummod	120,MΩ
R6748	T15052	T15051	punct	–,120
R6749	T15053	T15049	punct	", ",MΩ
R675	T2068	T2069	det	the,onset
R6750	T15054	T15055	compound	3M,KCl
R6751	T15055	T15049	appos	KCl,MΩ
R6752	T15056	T15049	punct	),MΩ
R6753	T15057	T15035	punct	.,made
R6754	T15059	T15060	amod	Average,responses
R6755	T15060	T15061	nsubjpass	responses,acquired
R6756	T15062	T15063	punct	(,trials
R6757	T15063	T15060	parataxis	trials,responses
R6758	T15064	T15065	quantmod	10,20
R6759	T15065	T15063	nummod	20,trials
R676	T2069	T2067	pobj	onset,in
R6760	T15066	T15065	punct	–,20
R6761	T15067	T15063	punct	),trials
R6762	T15068	T15060	prep	from,responses
R6763	T15069	T15070	compound	suprathreshold,stimulation
R6764	T15070	T15068	pobj	stimulation,from
R6765	T15071	T15070	compound	nerve,stimulation
R6766	T15072	T15073	punct	(,strength
R6767	T15073	T15070	parataxis	strength,stimulation
R6768	T15074	T15073	nummod	1.5,strength
R6769	T15075	T15074	quantmod	times,1.5
R677	T1958	T1961	nsubj	proteins,control
R6770	T15076	T15073	det	the,strength
R6771	T15077	T15078	dep	that,evokes
R6772	T15078	T15073	relcl	evokes,strength
R6773	T15079	T15080	amod	maximal,response
R6774	T15080	T15078	dobj	response,evokes
R6775	T15081	T15080	amod	monosynaptic,response
R6776	T15082	T15073	punct	),strength
R6777	T15083	T15070	prep	of,stimulation
R6778	T15084	T15085	det	the,nerve
R6779	T15085	T15083	pobj	nerve,of
R678	T2070	T2069	prep	of,onset
R6780	T15086	T15085	compound	quadriceps,nerve
R6781	T15087	T15061	auxpass	were,acquired
R6782	T15088	T15061	prep	with,acquired
R6783	T15089	T15090	compound	LTP,software
R6784	T15090	T15088	pobj	software,with
R6785	T15091	T15092	punct	[,49
R6786	T15092	T15061	parataxis	49,acquired
R6787	T15093	T15092	punct	],49
R6788	T15094	T15061	punct	.,acquired
R6789	T15096	T15097	advmod	Only,cells
R679	T2071	T2072	compound	ETS,signaling
R6790	T15097	T15098	nsubjpass	cells,considered
R6791	T15099	T15097	prep	with,cells
R6792	T15100	T15101	amod	stable,potentials
R6793	T15101	T15099	pobj	potentials,with
R6794	T15102	T15101	compound	resting,potentials
R6795	T15103	T15104	advmod	more,negative
R6796	T15104	T15101	amod	negative,potentials
R6797	T15105	T15104	prep	than,negative
R6798	T15106	T15107	punct	−,50
R6799	T15107	T15108	nummod	50,mV
R680	T2072	T2070	pobj	signaling,of
R6800	T15108	T15105	pobj	mV,than
R6801	T15109	T15098	auxpass	were,considered
R6802	T15110	T15098	prep	for,considered
R6803	T15111	T15110	pobj	analysis,for
R6804	T15112	T15098	punct	.,considered
R6805	T15114	T15115	amod	Monosynaptic,amplitudes
R6806	T15115	T15116	nsubjpass	amplitudes,determined
R6807	T15117	T15116	auxpass	were,determined
R6808	T15118	T15116	advmod	offline,determined
R6809	T15119	T15116	advcl	using,determined
R681	T1959	T1958	nummod	two,proteins
R6810	T15120	T15121	compound	custom,routines
R6811	T15121	T15119	dobj	routines,using
R6812	T15122	T15119	prep	in,using
R6813	T15123	T15124	det	the,environment
R6814	T15124	T15122	pobj	environment,in
R6815	T15125	T15124	compound	Matlab,environment
R6816	T15126	T15127	punct	(,Mathworks
R6817	T15127	T15124	parataxis	Mathworks,environment
R6818	T15128	T15127	det	The,Mathworks
R6819	T15129	T15127	punct	", ",Mathworks
R682	T2073	T2072	prep	for,signaling
R6820	T15130	T15127	npadvmod	Natick,Mathworks
R6821	T15131	T15127	punct	", ",Mathworks
R6822	T15132	T15127	npadvmod	Massachusetts,Mathworks
R6823	T15133	T15127	punct	", ",Mathworks
R6824	T15134	T15135	compound	United,States
R6825	T15135	T15127	npadvmod	States,Mathworks
R6826	T15136	T15127	punct	),Mathworks
R6827	T15137	T15138	mark	as,described
R6828	T15138	T15119	advcl	described,using
R6829	T15139	T15138	advmod	previously,described
R683	T2074	T2075	amod	neuronal,maturation
R6830	T15140	T15141	punct	[,48
R6831	T15141	T15138	parataxis	48,described
R6832	T15142	T15141	punct	],48
R6833	T15143	T15116	punct	.,determined
R684	T1960	T1958	compound	ETS,proteins
R685	T1962	T1963	amod	late,aspects
R686	T1963	T1961	dobj	aspects,control
R687	T1964	T1963	prep	of,aspects
R688	T1965	T1966	amod	spinal,circuit
R689	T2075	T2073	pobj	maturation,for
R690	T1966	T1968	compound	circuit,assembly
R691	T2076	T2045	punct	.,raises
R692	T2078	T2079	aux	Would,direct
R693	T1967	T1966	amod	monosynaptic,circuit
R694	T2080	T2081	amod	precocious,expression
R695	T1968	T1964	pobj	assembly,of
R696	T2081	T2079	nsubj	expression,direct
R697	T2082	T2081	prep	of,expression
R698	T2182	T2181	amod	spinal,cord
R699	T2083	T2084	compound	ETS,proteins
R700	T2084	T2082	pobj	proteins,of
R701	T2085	T2081	prep	in,expression
R702	T2086	T2087	amod	post-mitotic,neurons
R703	T2087	T2085	pobj	neurons,in
R704	T2088	T2079	advmod	also,direct
R705	T2089	T2090	det	the,programs
R706	T2090	T2079	dobj	programs,direct
R707	T2183	T2156	punct	.,find
R708	T2091	T2090	amod	appropriate,programs
R709	T2092	T2093	amod	sensory,neuron
R710	T2185	T2186	amod	Precocious,initiation
R711	T2093	T2090	nmod	neuron,programs
R712	T2094	T2090	amod	developmental,programs
R713	T2095	T2079	punct	?,direct
R714	T2186	T2187	nsubj	initiation,leads
R715	T2097	T2098	prep	In,used
R716	T2188	T2186	prep	of,initiation
R717	T2099	T2100	det	this,study
R718	T2100	T2097	pobj	study,In
R719	T2101	T2098	punct	", ",used
R720	T2102	T2098	nsubj	we,used
R721	T2103	T2098	aux	have,used
R722	T2189	T2190	compound	ETS,signaling
R723	T2104	T2105	compound	mouse,genetics
R724	T2105	T2098	dobj	genetics,used
R725	T2106	T2107	aux	to,test
R726	T2190	T2188	pobj	signaling,of
R727	T2107	T2098	advcl	test,used
R728	T2108	T2109	det	this,idea
R729	T2109	T2107	dobj	idea,test
R730	T2191	T2190	prep	in,signaling
R731	T2110	T2109	amod	general,idea
R732	T2111	T2107	punct	", ",test
R733	T2192	T2193	amod	post-mitotic,neurons
R734	T2112	T2107	prep	by,test
R735	T2113	T2112	pcomp	investigating,by
R736	T2114	T2115	mark	whether,is
R737	T2193	T2191	pobj	neurons,in
R738	T2115	T2113	ccomp	is,investigating
R739	T2116	T2117	det	the,timing
R740	T2117	T2115	nsubj	timing,is
R741	T2194	T2193	compound	DRG,neurons
R742	T2118	T2117	amod	precise,timing
R743	T2119	T2117	prep	of,timing
R744	T2120	T2119	pobj	onset,of
R745	T2195	T2187	prep	to,leads
R746	T2121	T2120	prep	of,onset
R747	T2122	T2123	compound	ETS,signaling
R748	T2196	T2197	amod	abnormal,differentiation
R749	T2123	T2121	pobj	signaling,of
R750	T2124	T2125	compound	transcription,factor
R751	T2125	T2123	compound	factor,signaling
R752	T2197	T2195	pobj	differentiation,to
R753	T2126	T2115	acomp	essential,is
R754	T2127	T2126	prep	for,essential
R755	T2198	T2197	compound	DRG,differentiation
R756	T2199	T2197	compound	neuron,differentiation
R757	T2128	T2129	amod	normal,development
R758	T2129	T2127	pobj	development,for
R759	T2200	T2197	acl	characterized,differentiation
R760	T2130	T2131	amod	sensory,neuron
R761	T2131	T2129	compound	neuron,development
R762	T2201	T2200	agent	by,characterized
R763	T2132	T2098	punct	.,used
R764	T2134	T2135	nsubj	We,assessed
R765	T2202	T2203	npadvmod	neurotrophin,independent
R766	T2136	T2135	aux	have,assessed
R767	T2203	T2205	amod	independent,outgrowth
R768	T2137	T2138	det	the,effects
R769	T2138	T2135	dobj	effects,assessed
R770	T2204	T2203	punct	-,independent
R771	T2139	T2138	amod	biological,effects
R772	T2140	T2138	prep	of,effects
R773	T2141	T2140	pcomp	inducing,of
R774	T2142	T2143	compound	ETS,signaling
R775	T2143	T2141	dobj	signaling,inducing
R776	T2205	T2201	pobj	outgrowth,by
R777	T2144	T2145	preconj	either,at
R778	T2206	T2205	compound	neurite,outgrowth
R779	T2145	T2141	prep	at,inducing
R780	T2146	T2147	det	the,time
R781	T2147	T2145	pobj	time,at
R782	T2207	T2205	cc	and,outgrowth
R783	T2148	T2147	amod	correct,time
R784	T2149	T2147	amod	developmental,time
R785	T2150	T2145	punct	", ",at
R786	T2208	T2209	amod	inappropriate,profiles
R787	T2151	T2145	cc	or,at
R788	T2152	T2145	conj	precociously,at
R789	T2153	T2135	punct	.,assessed
R790	T2209	T2205	conj	profiles,outgrowth
R791	T2155	T2156	nsubj	We,find
R792	T2210	T2209	prep	of,profiles
R793	T2157	T2158	mark	that,is
R794	T2158	T2156	ccomp	is,find
R795	T2211	T2212	compound	gene,expression
R796	T2159	T2158	prep	within,is
R797	T2160	T2161	amod	proprioceptive,neurons
R798	T2161	T2159	pobj	neurons,within
R799	T2212	T2210	pobj	expression,of
R800	T2162	T2161	amod	sensory,neurons
R801	T2163	T2158	punct	", ",is
R802	T2164	T2165	det	the,onset
R803	T2213	T2187	punct	.,leads
R804	T2165	T2158	nsubj	onset,is
R805	T2166	T2165	amod	late,onset
R806	T2215	T2216	poss	Our,findings
R807	T2167	T2165	prep	of,onset
R808	T2168	T2169	compound	ETS,signaling
R809	T2169	T2167	pobj	signaling,of
R810	T2170	T2158	acomp	essential,is
R811	T2171	T2170	prep	for,essential
R812	T2172	T2173	det	the,establishment
R813	T2173	T2171	pobj	establishment,for
R814	T2216	T2217	nsubj	findings,reveal
R815	T2174	T2173	prep	of,establishment
R816	T2175	T2176	amod	normal,projections
R817	T2176	T2174	pobj	projections,of
R818	T2218	T2219	mark	that,provide
R819	T2177	T2178	amod	sensory,afferent
R820	T2178	T2176	nmod	afferent,projections
R821	T2179	T2173	prep	in,establishment
R822	T2180	T2181	det	the,cord
R823	T2219	T2217	ccomp	provide,reveal
R824	T2181	T2179	pobj	cord,in
R825	T2220	T2221	npadvmod	target,triggered
R826	T2221	T2223	amod	triggered,signals
R827	T2222	T2221	punct	-,triggered
R828	T2223	T2219	nsubj	signals,provide
R829	T2224	T2223	amod	inductive,signals
R830	T2225	T2226	det	an,means
R831	T2226	T2219	dobj	means,provide
R832	T2227	T2226	amod	effective,means
R833	T2228	T2226	prep	of,means
R834	T2229	T2228	pcomp	ensuring,of
R835	T2230	T2231	det	the,onset
R836	T2231	T2229	dobj	onset,ensuring
R837	T2232	T2231	amod	late,onset
R838	T2233	T2231	prep	of,onset
R839	T2234	T2233	pobj	expression,of
R840	T2235	T2234	prep	of,expression
R841	T2236	T2237	compound	transcription,factors
R842	T2237	T2235	pobj	factors,of
R843	T2238	T2219	punct	", ",provide
R844	T2239	T2219	cc	and,provide
R845	T2240	T2241	advmod	thus,sequence
R846	T2241	T2219	conj	sequence,provide
R847	T2242	T2241	det	an,sequence
R848	T2243	T2241	amod	orderly,sequence
R849	T2244	T2241	amod	temporal,sequence
R850	T2245	T2241	amod	transcriptional,sequence
R851	T2246	T2247	dep	that,is
R852	T2247	T2241	relcl	is,sequence
R853	T2248	T2247	acomp	crucial,is
R854	T2249	T2248	prep	for,crucial
R855	T2250	T2251	amod	neuronal,maturation
R856	T2251	T2249	pobj	maturation,for
R857	T2252	T2251	cc	and,maturation
R858	T2253	T2254	compound	circuit,assembly
R859	T2254	T2251	conj	assembly,maturation
R860	T2255	T2217	punct	.,reveal
R864	T2468	T2469	aux	To,test
R865	T2469	T2470	advcl	test,studied
R866	T2471	T2472	det	the,hypothesis
R867	T2472	T2469	dobj	hypothesis,test
R868	T2473	T2474	mark	that,is
R869	T2474	T2472	acl	is,hypothesis
R870	T2475	T2476	det	a,delay
R871	T2476	T2474	nsubj	delay,is
R872	T2477	T2476	amod	temporal,delay
R873	T2478	T2476	prep	in,delay
R874	T2479	T2480	det	the,onset
R875	T2480	T2478	pobj	onset,in
R876	T2481	T2480	prep	of,onset
R877	T2482	T2483	amod	transcriptional,programs
R878	T2483	T2481	pobj	programs,of
R879	T2484	T2474	acomp	crucial,is
R880	T2485	T2484	prep	for,crucial
R881	T2486	T2487	det	the,control
R882	T2487	T2485	pobj	control,for
R883	T2488	T2487	prep	of,control
R884	T2489	T2490	amod	appropriate,maturation
R885	T2490	T2488	pobj	maturation,of
R886	T2491	T2490	amod	neuronal,maturation
R887	T2492	T2470	punct	", ",studied
R888	T2493	T2470	nsubj	we,studied
R889	T2494	T2495	det	the,development
R890	T2495	T2470	dobj	development,studied
R891	T2496	T2495	prep	of,development
R892	T2497	T2498	amod	proprioceptive,neurons
R893	T2498	T2496	pobj	neurons,of
R894	T2499	T2498	compound	DRG,neurons
R895	T2500	T2470	punct	", ",studied
R896	T2501	T2502	mark	since,identified
R897	T2502	T2470	advcl	identified,studied
R898	T2503	T2504	amod	transcriptional,effectors
R899	T2504	T2502	nsubjpass	effectors,identified
R900	T2505	T2504	acl	regulated,effectors
R901	T2506	T2505	agent	by,regulated
R902	T2507	T2508	npadvmod	target,derived
R903	T2508	T2510	amod	derived,signals
R904	T2509	T2508	punct	-,derived
R905	T2510	T2506	pobj	signals,by
R906	T2511	T2510	punct	", ",signals
R907	T2512	T2513	advmod	as,as
R908	T2513	T2510	cc	as,signals
R909	T2514	T2513	advmod	well,as
R910	T2515	T2510	conj	some,signals
R911	T2516	T2515	prep	of,some
R912	T2517	T2518	poss	their,actions
R913	T2518	T2516	pobj	actions,of
R914	T2519	T2518	amod	downstream,actions
R915	T2520	T2518	amod	biological,actions
R916	T2521	T2502	punct	", ",identified
R917	T2522	T2502	aux	have,identified
R918	T2523	T2502	auxpass	been,identified
R919	T2524	T2502	prep	for,identified
R920	T2525	T2526	det	these,neurons
R921	T2526	T2524	pobj	neurons,for
R922	T2527	T2470	punct	.,studied
R923	T2529	T2530	nsubj	Er81,controls
R924	T2531	T2532	amod	proprioceptive,connectivity
R925	T2532	T2530	dobj	connectivity,controls
R926	T2533	T2532	amod	afferent,connectivity
R927	T2534	T2535	punct	[,14
R928	T2535	T2530	parataxis	14,controls
R929	T2536	T2535	punct	],14
R930	T2537	T2530	punct	", ",controls
R931	T2538	T2530	cc	and,controls
R932	T2539	T2540	nsubj	we,sought
R933	T2540	T2530	conj	sought,controls
R934	T2541	T2540	advmod	therefore,sought
R935	T2542	T2543	aux	to,identify
R936	T2543	T2540	xcomp	identify,sought
R937	T2544	T2545	det	an,regulator
R938	T2545	T2543	dobj	regulator,identify
R939	T2546	T2545	nmod	ETS,regulator
R940	T2547	T2545	amod	transcriptional,regulator
R941	T2548	T2549	dep	that,is
R942	T2549	T2545	relcl	is,regulator
R943	T2550	T2549	punct	", ",is
R944	T2551	T2552	advmod	when,expressed
R945	T2552	T2549	advcl	expressed,is
R946	T2553	T2552	prep	over,expressed
R947	T2554	T2555	det	the,course
R948	T2555	T2553	pobj	course,over
R949	T2556	T2555	amod	normal,course
R950	T2557	T2555	compound	time,course
R951	T2558	T2555	prep	of,course
R952	T2559	T2560	compound	Er81,expression
R953	T2560	T2558	pobj	expression,of
R954	T2561	T2549	punct	", ",is
R955	T2562	T2549	acomp	able,is
R956	T2563	T2564	aux	to,substitute
R957	T2564	T2562	xcomp	substitute,able
R958	T2565	T2564	prep	for,substitute
R959	T2566	T2567	compound	Er81,function
R960	T2567	T2565	pobj	function,for
R961	T2568	T2564	prep	within,substitute
R962	T2569	T2570	nmod	group,Ia
R963	T2570	T2571	nmod	Ia,neurons
R964	T2571	T2568	pobj	neurons,within
R965	T2572	T2571	amod	afferent,neurons
R966	T2573	T2571	amod	sensory,neurons
R967	T2574	T2540	punct	.,sought
R968	T2576	T2577	prep	With,designed
R969	T2578	T2579	det	this,point
R970	T2579	T2576	pobj	point,With
R971	T2580	T2579	compound	reference,point
R972	T2581	T2577	punct	", ",designed
R973	T2582	T2577	nsubj	we,designed
R974	T2583	T2577	advmod	then,designed
R975	T2584	T2577	dobj	experiments,designed
R976	T2585	T2586	aux	to,examine
R977	T2586	T2584	advcl	examine,experiments
R978	T2587	T2588	det	the,effects
R979	T2588	T2586	dobj	effects,examine
R980	T2589	T2588	prep	of,effects
R981	T2590	T2591	amod	precocious,expression
R982	T2591	T2589	pobj	expression,of
R983	T2592	T2591	amod	post-mitotic,expression
R984	T2593	T2591	prep	of,expression
R985	T2594	T2595	det	the,factor
R986	T2595	T2593	pobj	factor,of
R987	T2596	T2595	amod	same,factor
R988	T2597	T2595	compound	ETS,factor
R989	T2598	T2595	compound	transcription,factor
R990	T2599	T2588	prep	on,effects
R991	T2600	T2601	amod	sensory,neuron
R992	T2601	T2602	compound	neuron,differentiation
R993	T2602	T2599	pobj	differentiation,on
R994	T2603	T2577	punct	.,designed
R999	T3609	T3610	compound	EWS,Pea3

PMC:1084331 / 1222-51048 JSONTXT 6 Projects

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PMC:1084331 / 1222-51048 JSON TXT 6 Projects