Id |
Subject |
Object |
Predicate |
Lexical cue |
T40760 |
0-7 |
JJ |
denotes |
Primary |
T40762 |
8-13 |
NN |
denotes |
mouse |
T40763 |
14-24 |
NN |
denotes |
hepatocyte |
T40761 |
25-32 |
NNS |
denotes |
studies |
T40764 |
32-154 |
sentence |
denotes |
Primary cultures of mouse hepatocytes were prepared from male PGC-1α+/+ and PGC-1α−/− mice essentially as described [47]. |
T40765 |
33-40 |
JJ |
denotes |
Primary |
T40766 |
41-49 |
NNS |
denotes |
cultures |
T40768 |
50-52 |
IN |
denotes |
of |
T40769 |
53-58 |
NN |
denotes |
mouse |
T40770 |
59-70 |
NNS |
denotes |
hepatocytes |
T40771 |
71-75 |
VBD |
denotes |
were |
T40767 |
76-84 |
VBN |
denotes |
prepared |
T40772 |
85-89 |
IN |
denotes |
from |
T40773 |
90-94 |
JJ |
denotes |
male |
T40775 |
95-98 |
NN |
denotes |
PGC |
T40777 |
98-99 |
HYPH |
denotes |
- |
T40776 |
99-101 |
NN |
denotes |
1α |
T40778 |
101-102 |
SYM |
denotes |
+ |
T40779 |
102-103 |
HYPH |
denotes |
/ |
T40780 |
103-104 |
SYM |
denotes |
+ |
T40781 |
105-108 |
CC |
denotes |
and |
T40782 |
109-112 |
NN |
denotes |
PGC |
T40784 |
112-113 |
HYPH |
denotes |
- |
T40783 |
113-115 |
NN |
denotes |
1α |
T40785 |
115-116 |
SYM |
denotes |
− |
T40786 |
116-117 |
HYPH |
denotes |
/ |
T40787 |
117-118 |
SYM |
denotes |
− |
T40774 |
119-123 |
NNS |
denotes |
mice |
T40788 |
124-135 |
RB |
denotes |
essentially |
T40790 |
136-138 |
IN |
denotes |
as |
T40789 |
139-148 |
VBN |
denotes |
described |
T40791 |
149-150 |
-LRB- |
denotes |
[ |
T40792 |
150-152 |
CD |
denotes |
47 |
T40793 |
152-153 |
-RRB- |
denotes |
] |
T40794 |
153-154 |
. |
denotes |
. |
T40795 |
154-264 |
sentence |
denotes |
Fatty acid oxidation and triglyceride synthesis experiments were commenced 2–3 h after the cells were plated. |
T40796 |
155-160 |
JJ |
denotes |
Fatty |
T40797 |
161-165 |
NN |
denotes |
acid |
T40798 |
166-175 |
NN |
denotes |
oxidation |
T40800 |
176-179 |
CC |
denotes |
and |
T40801 |
180-192 |
NN |
denotes |
triglyceride |
T40802 |
193-202 |
NN |
denotes |
synthesis |
T40799 |
203-214 |
NNS |
denotes |
experiments |
T40804 |
215-219 |
VBD |
denotes |
were |
T40803 |
220-229 |
VBN |
denotes |
commenced |
T40805 |
230-231 |
CD |
denotes |
2 |
T40807 |
231-232 |
SYM |
denotes |
– |
T40806 |
232-233 |
CD |
denotes |
3 |
T40808 |
234-235 |
NN |
denotes |
h |
T40810 |
236-241 |
IN |
denotes |
after |
T40811 |
242-245 |
DT |
denotes |
the |
T40812 |
246-251 |
NNS |
denotes |
cells |
T40813 |
252-256 |
VBD |
denotes |
were |
T40809 |
257-263 |
VBN |
denotes |
plated |
T40814 |
263-264 |
. |
denotes |
. |
T40815 |
264-341 |
sentence |
denotes |
Triglyceride synthesis studies, were performed as previously described [47]. |
T40816 |
265-277 |
NN |
denotes |
Triglyceride |
T40817 |
278-287 |
NN |
denotes |
synthesis |
T40818 |
288-295 |
NNS |
denotes |
studies |
T40820 |
295-297 |
, |
denotes |
, |
T40821 |
297-301 |
VBD |
denotes |
were |
T40819 |
302-311 |
VBN |
denotes |
performed |
T40822 |
312-314 |
IN |
denotes |
as |
T40824 |
315-325 |
RB |
denotes |
previously |
T40823 |
326-335 |
VBN |
denotes |
described |
T40825 |
336-337 |
-LRB- |
denotes |
[ |
T40826 |
337-339 |
CD |
denotes |
47 |
T40827 |
339-340 |
-RRB- |
denotes |
] |
T40828 |
340-341 |
. |
denotes |
. |
T40829 |
341-481 |
sentence |
denotes |
Palmitate oxidation rates were quantified using [9,10-3H]-palmitic acid as described [48] and corrected for total cellular protein content. |
T40830 |
342-351 |
NN |
denotes |
Palmitate |
T40832 |
352-361 |
NN |
denotes |
oxidation |
T40831 |
362-367 |
NNS |
denotes |
rates |
T40834 |
368-372 |
VBD |
denotes |
were |
T40833 |
373-383 |
VBN |
denotes |
quantified |
T40835 |
384-389 |
VBG |
denotes |
using |
T40836 |
390-391 |
-LRB- |
denotes |
[ |
T40837 |
391-392 |
CD |
denotes |
9 |
T40839 |
392-393 |
, |
denotes |
, |
T40840 |
393-395 |
CD |
denotes |
10 |
T40841 |
395-396 |
SYM |
denotes |
- |
T40838 |
396-398 |
NN |
denotes |
3H |
T40843 |
398-399 |
-RRB- |
denotes |
] |
T40844 |
399-400 |
HYPH |
denotes |
- |
T40842 |
400-408 |
JJ |
denotes |
palmitic |
T40845 |
409-413 |
NN |
denotes |
acid |
T40846 |
414-416 |
IN |
denotes |
as |
T40847 |
417-426 |
VBN |
denotes |
described |
T40848 |
427-428 |
-LRB- |
denotes |
[ |
T40849 |
428-430 |
CD |
denotes |
48 |
T40850 |
430-431 |
-RRB- |
denotes |
] |
T40851 |
432-435 |
CC |
denotes |
and |
T40852 |
436-445 |
VBN |
denotes |
corrected |
T40853 |
446-449 |
IN |
denotes |
for |
T40854 |
450-455 |
JJ |
denotes |
total |
T40856 |
456-464 |
JJ |
denotes |
cellular |
T40857 |
465-472 |
NN |
denotes |
protein |
T40855 |
473-480 |
NN |
denotes |
content |
T40858 |
480-481 |
. |
denotes |
. |
T40859 |
481-636 |
sentence |
denotes |
For respiration studies, cells were spun down prior to plating and resuspended in a permeabilization buffer (described above) containing 50 μg/ml saponin. |
T40860 |
482-485 |
IN |
denotes |
For |
T40862 |
486-497 |
NN |
denotes |
respiration |
T40863 |
498-505 |
NNS |
denotes |
studies |
T40864 |
505-507 |
, |
denotes |
, |
T40865 |
507-512 |
NNS |
denotes |
cells |
T40866 |
513-517 |
VBD |
denotes |
were |
T40861 |
518-522 |
VBN |
denotes |
spun |
T40867 |
523-527 |
RP |
denotes |
down |
T40868 |
528-533 |
JJ |
denotes |
prior |
T40869 |
534-536 |
IN |
denotes |
to |
T40870 |
537-544 |
NN |
denotes |
plating |
T40871 |
545-548 |
CC |
denotes |
and |
T40872 |
549-560 |
VBN |
denotes |
resuspended |
T40873 |
561-563 |
IN |
denotes |
in |
T40874 |
564-565 |
DT |
denotes |
a |
T40876 |
566-582 |
NN |
denotes |
permeabilization |
T40875 |
583-589 |
NN |
denotes |
buffer |
T40877 |
590-591 |
-LRB- |
denotes |
( |
T40878 |
591-600 |
VBN |
denotes |
described |
T40879 |
601-606 |
RB |
denotes |
above |
T40880 |
606-607 |
-RRB- |
denotes |
) |
T40881 |
608-618 |
VBG |
denotes |
containing |
T40882 |
619-621 |
CD |
denotes |
50 |
T40883 |
622-624 |
NN |
denotes |
μg |
T40885 |
624-625 |
SYM |
denotes |
/ |
T40886 |
625-627 |
NN |
denotes |
ml |
T40884 |
628-635 |
NN |
denotes |
saponin |
T40887 |
635-636 |
. |
denotes |
. |
T40888 |
636-740 |
sentence |
denotes |
Respiration studies were performed in the presence of 5 mM succinate in the presence of 10 μM rotenone. |
T40889 |
637-648 |
NN |
denotes |
Respiration |
T40890 |
649-656 |
NNS |
denotes |
studies |
T40892 |
657-661 |
VBD |
denotes |
were |
T40891 |
662-671 |
VBN |
denotes |
performed |
T40893 |
672-674 |
IN |
denotes |
in |
T40894 |
675-678 |
DT |
denotes |
the |
T40895 |
679-687 |
NN |
denotes |
presence |
T40896 |
688-690 |
IN |
denotes |
of |
T40897 |
691-692 |
CD |
denotes |
5 |
T40898 |
693-695 |
NN |
denotes |
mM |
T40899 |
696-705 |
NN |
denotes |
succinate |
T40900 |
706-708 |
IN |
denotes |
in |
T40901 |
709-712 |
DT |
denotes |
the |
T40902 |
713-721 |
NN |
denotes |
presence |
T40903 |
722-724 |
IN |
denotes |
of |
T40904 |
725-727 |
CD |
denotes |
10 |
T40905 |
728-730 |
NN |
denotes |
μM |
T40906 |
731-739 |
NN |
denotes |
rotenone |
T40907 |
739-740 |
. |
denotes |
. |
T40908 |
740-811 |
sentence |
denotes |
Respiration rates were expressed as nmol O2 · min−1 · mg of protein−1. |
T40909 |
741-752 |
NN |
denotes |
Respiration |
T40910 |
753-758 |
NNS |
denotes |
rates |
T40912 |
759-763 |
VBD |
denotes |
were |
T40911 |
764-773 |
VBN |
denotes |
expressed |
T40913 |
774-776 |
IN |
denotes |
as |
T40914 |
777-781 |
NN |
denotes |
nmol |
T40915 |
782-784 |
NN |
denotes |
O2 |
T40916 |
785-786 |
SYM |
denotes |
· |
T40917 |
787-790 |
NN |
denotes |
min |
T40918 |
790-791 |
SYM |
denotes |
− |
T40919 |
791-792 |
CD |
denotes |
1 |
T40920 |
793-794 |
SYM |
denotes |
· |
T40921 |
795-797 |
NN |
denotes |
mg |
T40922 |
798-800 |
IN |
denotes |
of |
T40923 |
801-808 |
NN |
denotes |
protein |
T40924 |
808-809 |
SYM |
denotes |
− |
T40925 |
809-810 |
CD |
denotes |
1 |
T40926 |
810-811 |
. |
denotes |
. |
R10388 |
T40760 |
T40761 |
amod |
Primary,studies |
R10389 |
T40762 |
T40763 |
compound |
mouse,hepatocyte |
R10390 |
T40763 |
T40761 |
compound |
hepatocyte,studies |
R10391 |
T40765 |
T40766 |
amod |
Primary,cultures |
R10392 |
T40766 |
T40767 |
nsubjpass |
cultures,prepared |
R10393 |
T40768 |
T40766 |
prep |
of,cultures |
R10394 |
T40769 |
T40770 |
compound |
mouse,hepatocytes |
R10395 |
T40770 |
T40768 |
pobj |
hepatocytes,of |
R10396 |
T40771 |
T40767 |
auxpass |
were,prepared |
R10397 |
T40772 |
T40767 |
prep |
from,prepared |
R10398 |
T40773 |
T40774 |
amod |
male,mice |
R10399 |
T40774 |
T40772 |
pobj |
mice,from |
R10400 |
T40775 |
T40776 |
nmod |
PGC,1α |
R10401 |
T40776 |
T40774 |
nmod |
1α,mice |
R10402 |
T40777 |
T40776 |
punct |
-,1α |
R10403 |
T40778 |
T40776 |
punct |
+,1α |
R10404 |
T40779 |
T40776 |
punct |
/,1α |
R10405 |
T40780 |
T40776 |
punct |
+,1α |
R10406 |
T40781 |
T40776 |
cc |
and,1α |
R10407 |
T40782 |
T40783 |
compound |
PGC,1α |
R10408 |
T40783 |
T40776 |
conj |
1α,1α |
R10409 |
T40784 |
T40783 |
punct |
-,1α |
R10410 |
T40785 |
T40783 |
punct |
−,1α |
R10411 |
T40786 |
T40783 |
punct |
/,1α |
R10412 |
T40787 |
T40783 |
punct |
−,1α |
R10413 |
T40788 |
T40789 |
advmod |
essentially,described |
R10414 |
T40789 |
T40767 |
advcl |
described,prepared |
R10415 |
T40790 |
T40789 |
mark |
as,described |
R10416 |
T40791 |
T40792 |
punct |
[,47 |
R10417 |
T40792 |
T40789 |
parataxis |
47,described |
R10418 |
T40793 |
T40792 |
punct |
],47 |
R10419 |
T40794 |
T40767 |
punct |
.,prepared |
R10420 |
T40796 |
T40797 |
amod |
Fatty,acid |
R10421 |
T40797 |
T40798 |
nmod |
acid,oxidation |
R10422 |
T40798 |
T40799 |
nmod |
oxidation,experiments |
R10423 |
T40799 |
T40803 |
nsubjpass |
experiments,commenced |
R10424 |
T40800 |
T40798 |
cc |
and,oxidation |
R10425 |
T40801 |
T40802 |
compound |
triglyceride,synthesis |
R10426 |
T40802 |
T40798 |
conj |
synthesis,oxidation |
R10427 |
T40804 |
T40803 |
auxpass |
were,commenced |
R10428 |
T40805 |
T40806 |
quantmod |
2,3 |
R10429 |
T40806 |
T40808 |
nummod |
3,h |
R10430 |
T40807 |
T40806 |
punct |
–,3 |
R10431 |
T40808 |
T40809 |
npadvmod |
h,plated |
R10432 |
T40809 |
T40803 |
advcl |
plated,commenced |
R10433 |
T40810 |
T40809 |
mark |
after,plated |
R10434 |
T40811 |
T40812 |
det |
the,cells |
R10435 |
T40812 |
T40809 |
nsubjpass |
cells,plated |
R10436 |
T40813 |
T40809 |
auxpass |
were,plated |
R10437 |
T40814 |
T40803 |
punct |
.,commenced |
R10438 |
T40816 |
T40817 |
compound |
Triglyceride,synthesis |
R10439 |
T40817 |
T40818 |
compound |
synthesis,studies |
R10440 |
T40818 |
T40819 |
nsubjpass |
studies,performed |
R10441 |
T40820 |
T40819 |
punct |
", ",performed |
R10442 |
T40821 |
T40819 |
auxpass |
were,performed |
R10443 |
T40822 |
T40823 |
mark |
as,described |
R10444 |
T40823 |
T40819 |
advcl |
described,performed |
R10445 |
T40824 |
T40823 |
advmod |
previously,described |
R10446 |
T40825 |
T40826 |
punct |
[,47 |
R10447 |
T40826 |
T40823 |
parataxis |
47,described |
R10448 |
T40827 |
T40826 |
punct |
],47 |
R10449 |
T40828 |
T40819 |
punct |
.,performed |
R10450 |
T40830 |
T40831 |
compound |
Palmitate,rates |
R10451 |
T40831 |
T40833 |
nsubjpass |
rates,quantified |
R10452 |
T40832 |
T40831 |
compound |
oxidation,rates |
R10453 |
T40834 |
T40833 |
auxpass |
were,quantified |
R10454 |
T40835 |
T40833 |
advcl |
using,quantified |
R10455 |
T40836 |
T40835 |
punct |
[,using |
R10456 |
T40837 |
T40838 |
nummod |
9,3H |
R10457 |
T40838 |
T40842 |
npadvmod |
3H,palmitic |
R10458 |
T40839 |
T40838 |
punct |
",",3H |
R10459 |
T40840 |
T40838 |
nummod |
10,3H |
R10460 |
T40841 |
T40838 |
punct |
-,3H |
R10461 |
T40842 |
T40845 |
amod |
palmitic,acid |
R10462 |
T40843 |
T40842 |
punct |
],palmitic |
R10463 |
T40844 |
T40842 |
punct |
-,palmitic |
R10464 |
T40845 |
T40835 |
dobj |
acid,using |
R10465 |
T40846 |
T40847 |
mark |
as,described |
R10466 |
T40847 |
T40835 |
advcl |
described,using |
R10467 |
T40848 |
T40849 |
punct |
[,48 |
R10468 |
T40849 |
T40847 |
parataxis |
48,described |
R10469 |
T40850 |
T40849 |
punct |
],48 |
R10470 |
T40851 |
T40833 |
cc |
and,quantified |
R10471 |
T40852 |
T40833 |
conj |
corrected,quantified |
R10472 |
T40853 |
T40852 |
prep |
for,corrected |
R10473 |
T40854 |
T40855 |
amod |
total,content |
R10474 |
T40855 |
T40853 |
pobj |
content,for |
R10475 |
T40856 |
T40855 |
amod |
cellular,content |
R10476 |
T40857 |
T40855 |
compound |
protein,content |
R10477 |
T40858 |
T40833 |
punct |
.,quantified |
R10478 |
T40860 |
T40861 |
prep |
For,spun |
R10479 |
T40862 |
T40863 |
compound |
respiration,studies |
R10480 |
T40863 |
T40860 |
pobj |
studies,For |
R10481 |
T40864 |
T40861 |
punct |
", ",spun |
R10482 |
T40865 |
T40861 |
nsubjpass |
cells,spun |
R10483 |
T40866 |
T40861 |
auxpass |
were,spun |
R10484 |
T40867 |
T40861 |
prt |
down,spun |
R10485 |
T40868 |
T40869 |
amod |
prior,to |
R10486 |
T40869 |
T40861 |
prep |
to,spun |
R10487 |
T40870 |
T40869 |
pobj |
plating,to |
R10488 |
T40871 |
T40861 |
cc |
and,spun |
R10489 |
T40872 |
T40861 |
conj |
resuspended,spun |
R10490 |
T40873 |
T40872 |
prep |
in,resuspended |
R10491 |
T40874 |
T40875 |
det |
a,buffer |
R10492 |
T40875 |
T40873 |
pobj |
buffer,in |
R10493 |
T40876 |
T40875 |
compound |
permeabilization,buffer |
R10494 |
T40877 |
T40875 |
punct |
(,buffer |
R10495 |
T40878 |
T40875 |
acl |
described,buffer |
R10496 |
T40879 |
T40878 |
advmod |
above,described |
R10497 |
T40880 |
T40875 |
punct |
),buffer |
R10498 |
T40881 |
T40875 |
acl |
containing,buffer |
R10499 |
T40882 |
T40883 |
nummod |
50,μg |
R10500 |
T40883 |
T40884 |
nmod |
μg,saponin |
R10501 |
T40884 |
T40881 |
dobj |
saponin,containing |
R10502 |
T40885 |
T40886 |
punct |
/,ml |
R10503 |
T40886 |
T40883 |
prep |
ml,μg |
R10504 |
T40887 |
T40861 |
punct |
.,spun |
R10505 |
T40889 |
T40890 |
compound |
Respiration,studies |
R10506 |
T40890 |
T40891 |
nsubjpass |
studies,performed |
R10507 |
T40892 |
T40891 |
auxpass |
were,performed |
R10508 |
T40893 |
T40891 |
prep |
in,performed |
R10509 |
T40894 |
T40895 |
det |
the,presence |
R10510 |
T40895 |
T40893 |
pobj |
presence,in |
R10511 |
T40896 |
T40895 |
prep |
of,presence |
R10512 |
T40897 |
T40898 |
nummod |
5,mM |
R10513 |
T40898 |
T40899 |
compound |
mM,succinate |
R10514 |
T40899 |
T40896 |
pobj |
succinate,of |
R10515 |
T40900 |
T40891 |
prep |
in,performed |
R10516 |
T40901 |
T40902 |
det |
the,presence |
R10517 |
T40902 |
T40900 |
pobj |
presence,in |
R10518 |
T40903 |
T40902 |
prep |
of,presence |
R10519 |
T40904 |
T40905 |
nummod |
10,μM |
R10520 |
T40905 |
T40906 |
compound |
μM,rotenone |
R10521 |
T40906 |
T40903 |
pobj |
rotenone,of |
R10522 |
T40907 |
T40891 |
punct |
.,performed |
R10523 |
T40909 |
T40910 |
compound |
Respiration,rates |
R10524 |
T40910 |
T40911 |
nsubjpass |
rates,expressed |
R10525 |
T40912 |
T40911 |
auxpass |
were,expressed |
R10526 |
T40913 |
T40911 |
prep |
as,expressed |
R10527 |
T40914 |
T40915 |
compound |
nmol,O2 |
R10528 |
T40915 |
T40913 |
pobj |
O2,as |
R10529 |
T40916 |
T40915 |
punct |
·,O2 |
R10530 |
T40917 |
T40915 |
appos |
min,O2 |
R10531 |
T40918 |
T40919 |
punct |
−,1 |
R10532 |
T40919 |
T40917 |
nummod |
1,min |
R10533 |
T40920 |
T40915 |
punct |
·,O2 |
R10534 |
T40921 |
T40915 |
appos |
mg,O2 |
R10535 |
T40922 |
T40921 |
prep |
of,mg |
R10536 |
T40923 |
T40922 |
pobj |
protein,of |
R10537 |
T40924 |
T40925 |
punct |
−,1 |
R10538 |
T40925 |
T40921 |
nummod |
1,mg |
R10539 |
T40926 |
T40911 |
punct |
.,expressed |