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PMC:1064854 / 62714-64119 JSONTXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T38202 0-13 JJ denotes Mitochondrial
T38203 14-25 NN denotes respiration
T38205 26-29 VBD denotes was
T38204 30-38 VBN denotes assessed
T38206 39-41 IN denotes in
T38207 42-49 NN denotes saponin
T38209 49-50 HYPH denotes -
T38208 50-57 VBN denotes skinned
T38211 58-64 NN denotes soleus
T38210 65-71 NNS denotes fibers
T38212 72-76 IN denotes with
T38213 77-86 NN denotes succinate
T38214 87-89 IN denotes as
T38215 90-99 NN denotes substrate
T38216 100-103 CC denotes and
T38217 104-106 IN denotes in
T38218 107-110 DT denotes the
T38219 111-119 NN denotes presence
T38220 120-122 IN denotes of
T38221 123-131 NN denotes rotenone
T38222 132-134 IN denotes as
T38224 135-145 RB denotes previously
T38223 146-155 VBN denotes described
T38225 156-157 -LRB- denotes [
T38226 157-159 CD denotes 44
T38227 159-160 -RRB- denotes ]
T38228 160-161 . denotes .
T38229 161-259 sentence denotes In brief, 3-mo-old female mice were anesthetized with chloral hydrate (400 mg/kg of body weight).
T38230 162-164 IN denotes In
T38232 165-170 JJ denotes brief
T38233 170-172 , denotes ,
T38234 172-173 CD denotes 3
T38236 173-174 HYPH denotes -
T38235 174-176 NN denotes mo
T38238 176-177 HYPH denotes -
T38237 177-180 JJ denotes old
T38240 181-187 JJ denotes female
T38239 188-192 NNS denotes mice
T38241 193-197 VBD denotes were
T38231 198-210 VBN denotes anesthetized
T38242 211-215 IN denotes with
T38243 216-223 JJ denotes chloral
T38244 224-231 NN denotes hydrate
T38245 232-233 -LRB- denotes (
T38247 233-236 CD denotes 400
T38246 237-239 NN denotes mg
T38248 239-240 SYM denotes /
T38249 240-242 NN denotes kg
T38250 243-245 IN denotes of
T38251 246-250 NN denotes body
T38252 251-257 NN denotes weight
T38253 257-258 -RRB- denotes )
T38254 258-259 . denotes .
T38255 259-570 sentence denotes Soleus fibers were separated and then transferred to a buffer (2.77 mM K2Ca-EGTA, 7.23 mM K2EGTA, 6.56 mM MgCl2, 20 mM imidazole, 53.3 mM K-MES, 20 mM taurine, 5.3 mM ATP, 15 mM PCr, 3 mM KH2PO4, 0.5 mM DTT [pH 7.1]) supplemented with 50 μg/ml saponin and permeabilized for 30 min at 4 °C with gentle stirring.
T38256 260-266 NN denotes Soleus
T38257 267-273 NNS denotes fibers
T38259 274-278 VBD denotes were
T38258 279-288 VBN denotes separated
T38260 289-292 CC denotes and
T38261 293-297 RB denotes then
T38262 298-309 VBN denotes transferred
T38263 310-312 IN denotes to
T38264 313-314 DT denotes a
T38265 315-321 NN denotes buffer
T38266 322-323 -LRB- denotes (
T38267 323-327 CD denotes 2.77
T38268 328-330 NN denotes mM
T38270 331-335 NN denotes K2Ca
T38271 335-336 HYPH denotes -
T38269 336-340 NN denotes EGTA
T38272 340-342 , denotes ,
T38273 342-346 CD denotes 7.23
T38274 347-349 NN denotes mM
T38275 350-356 NN denotes K2EGTA
T38276 356-358 , denotes ,
T38277 358-362 CD denotes 6.56
T38278 363-365 NN denotes mM
T38279 366-371 NN denotes MgCl2
T38280 371-373 , denotes ,
T38281 373-375 CD denotes 20
T38282 376-378 NN denotes mM
T38283 379-388 NN denotes imidazole
T38284 388-390 , denotes ,
T38285 390-394 CD denotes 53.3
T38286 395-397 NN denotes mM
T38288 398-399 NN denotes K
T38289 399-400 HYPH denotes -
T38287 400-403 NN denotes MES
T38290 403-405 , denotes ,
T38291 405-407 CD denotes 20
T38292 408-410 NN denotes mM
T38293 411-418 NN denotes taurine
T38294 418-420 , denotes ,
T38295 420-423 CD denotes 5.3
T38296 424-426 NN denotes mM
T38297 427-430 NN denotes ATP
T38298 430-432 , denotes ,
T38299 432-434 CD denotes 15
T38300 435-437 NN denotes mM
T38301 438-441 NN denotes PCr
T38302 441-443 , denotes ,
T38303 443-444 CD denotes 3
T38304 445-447 NN denotes mM
T38305 448-454 NN denotes KH2PO4
T38306 454-456 , denotes ,
T38307 456-459 CD denotes 0.5
T38308 460-462 NN denotes mM
T38309 463-466 NN denotes DTT
T38310 467-468 -LRB- denotes [
T38311 468-470 NN denotes pH
T38312 471-474 CD denotes 7.1
T38313 474-475 -RRB- denotes ]
T38314 475-476 -RRB- denotes )
T38315 477-489 VBN denotes supplemented
T38316 490-494 IN denotes with
T38317 495-497 CD denotes 50
T38318 498-500 NN denotes μg
T38319 500-501 SYM denotes /
T38321 501-503 NN denotes ml
T38320 504-511 NN denotes saponin
T38322 512-515 CC denotes and
T38323 516-529 VBN denotes permeabilized
T38324 530-533 IN denotes for
T38325 534-536 CD denotes 30
T38326 537-540 NN denotes min
T38327 541-543 IN denotes at
T38328 544-545 CD denotes 4
T38329 546-548 NN denotes °C
T38330 549-553 IN denotes with
T38331 554-560 JJ denotes gentle
T38332 561-569 NN denotes stirring
T38333 569-570 . denotes .
T38334 570-761 sentence denotes Fibers were then washed twice for 10 min each (2.77 mM K2Ca-EGTA, 7.23 mM K2EGTA, 1.38 mM MgCl2, 20 mM imidazole, 100 mM K-MES, 20 mM taurine, 3 mM KH2PO4, 0.5 mM DTT, 2 mg/ml BSA [pH 7.1]).
T38335 571-577 NNS denotes Fibers
T38337 578-582 VBD denotes were
T38338 583-587 RB denotes then
T38336 588-594 VBN denotes washed
T38339 595-600 RB denotes twice
T38340 601-604 IN denotes for
T38341 605-607 CD denotes 10
T38342 608-611 NN denotes min
T38343 612-616 RB denotes each
T38344 617-618 -LRB- denotes (
T38346 618-622 CD denotes 2.77
T38347 623-625 NN denotes mM
T38348 626-630 NN denotes K2Ca
T38349 630-631 HYPH denotes -
T38345 631-635 NN denotes EGTA
T38350 635-637 , denotes ,
T38351 637-641 CD denotes 7.23
T38352 642-644 NN denotes mM
T38353 645-651 NN denotes K2EGTA
T38354 651-653 , denotes ,
T38355 653-657 CD denotes 1.38
T38356 658-660 NN denotes mM
T38357 661-666 NN denotes MgCl2
T38358 666-668 , denotes ,
T38359 668-670 CD denotes 20
T38360 671-673 NN denotes mM
T38361 674-683 NN denotes imidazole
T38362 683-685 , denotes ,
T38363 685-688 CD denotes 100
T38364 689-691 NN denotes mM
T38366 692-693 NN denotes K
T38367 693-694 HYPH denotes -
T38365 694-697 NN denotes MES
T38368 697-699 , denotes ,
T38369 699-701 CD denotes 20
T38370 702-704 NN denotes mM
T38371 705-712 NN denotes taurine
T38372 712-714 , denotes ,
T38373 714-715 CD denotes 3
T38374 716-718 NN denotes mM
T38375 719-725 NN denotes KH2PO4
T38376 725-727 , denotes ,
T38377 727-730 CD denotes 0.5
T38378 731-733 NN denotes mM
T38379 734-737 NN denotes DTT
T38380 737-739 , denotes ,
T38381 739-740 CD denotes 2
T38382 741-743 NN denotes mg
T38383 743-744 SYM denotes /
T38385 744-746 NN denotes ml
T38384 747-750 NN denotes BSA
T38386 751-752 -LRB- denotes [
T38387 752-754 NN denotes pH
T38388 755-758 CD denotes 7.1
T38389 758-759 -RRB- denotes ]
T38390 759-760 -RRB- denotes )
T38391 760-761 . denotes .
T38392 761-886 sentence denotes Respiration was measured at 25 °C using an optical probe (Oxygen FOXY Probe, Ocean Optics, Dunedin, Florida, United States).
T38393 762-773 NN denotes Respiration
T38395 774-777 VBD denotes was
T38394 778-786 VBN denotes measured
T38396 787-789 IN denotes at
T38397 790-792 CD denotes 25
T38398 793-795 NN denotes °C
T38399 796-801 VBG denotes using
T38400 802-804 DT denotes an
T38402 805-812 JJ denotes optical
T38401 813-818 NN denotes probe
T38403 819-820 -LRB- denotes (
T38405 820-826 NNP denotes Oxygen
T38406 827-831 NNP denotes FOXY
T38404 832-837 NNP denotes Probe
T38407 837-839 , denotes ,
T38408 839-844 NNP denotes Ocean
T38409 845-851 NNP denotes Optics
T38410 851-853 , denotes ,
T38411 853-860 NNP denotes Dunedin
T38412 860-862 , denotes ,
T38413 862-869 NNP denotes Florida
T38414 869-871 , denotes ,
T38415 871-877 NNP denotes United
T38416 878-884 NNP denotes States
T38417 884-885 -RRB- denotes )
T38418 885-886 . denotes .
T38419 886-1030 sentence denotes Following measurement of basal state 2 respiration, maximal (ADP-stimulated) state 3 respiration was determined by exposing fibers to 1 mM ADP.
T38420 887-896 VBG denotes Following
T38422 897-908 NN denotes measurement
T38423 909-911 IN denotes of
T38424 912-917 JJ denotes basal
T38426 918-923 NN denotes state
T38427 924-925 CD denotes 2
T38425 926-937 NN denotes respiration
T38428 937-939 , denotes ,
T38429 939-946 JJ denotes maximal
T38431 947-948 -LRB- denotes (
T38432 948-951 NN denotes ADP
T38434 951-952 HYPH denotes -
T38433 952-962 VBN denotes stimulated
T38435 962-963 -RRB- denotes )
T38436 964-969 NN denotes state
T38437 970-971 CD denotes 3
T38430 972-983 NN denotes respiration
T38438 984-987 VBD denotes was
T38421 988-998 VBN denotes determined
T38439 999-1001 IN denotes by
T38440 1002-1010 VBG denotes exposing
T38441 1011-1017 NNS denotes fibers
T38442 1018-1020 IN denotes to
T38443 1021-1022 CD denotes 1
T38444 1023-1025 NN denotes mM
T38445 1026-1029 NN denotes ADP
T38446 1029-1030 . denotes .
T38447 1030-1157 sentence denotes The integrity of the outer mitochondrial membrane was assessed by adding 8 μM exogenous cytochrome c to ADP-stimulated fibers.
T38448 1031-1034 DT denotes The
T38449 1035-1044 NN denotes integrity
T38451 1045-1047 IN denotes of
T38452 1048-1051 DT denotes the
T38454 1052-1057 JJ denotes outer
T38455 1058-1071 JJ denotes mitochondrial
T38453 1072-1080 NN denotes membrane
T38456 1081-1084 VBD denotes was
T38450 1085-1093 VBN denotes assessed
T38457 1094-1096 IN denotes by
T38458 1097-1103 VBG denotes adding
T38459 1104-1105 CD denotes 8
T38460 1106-1108 NN denotes μM
T38462 1109-1118 JJ denotes exogenous
T38463 1119-1129 NN denotes cytochrome
T38461 1130-1131 NN denotes c
T38464 1132-1134 IN denotes to
T38465 1135-1138 NN denotes ADP
T38467 1138-1139 HYPH denotes -
T38466 1139-1149 VBN denotes stimulated
T38468 1150-1156 NNS denotes fibers
T38469 1156-1157 . denotes .
T38470 1157-1247 sentence denotes State 4 respiration (uncoupled) was evaluated following addition of oligomycin (1 μg/ml).
T38471 1158-1163 NN denotes State
T38473 1164-1165 CD denotes 4
T38472 1166-1177 NN denotes respiration
T38475 1178-1179 -LRB- denotes (
T38476 1179-1188 JJ denotes uncoupled
T38477 1188-1189 -RRB- denotes )
T38478 1190-1193 VBD denotes was
T38474 1194-1203 VBN denotes evaluated
T38479 1204-1213 VBG denotes following
T38480 1214-1222 NN denotes addition
T38481 1223-1225 IN denotes of
T38482 1226-1236 NN denotes oligomycin
T38483 1237-1238 -LRB- denotes (
T38485 1238-1239 CD denotes 1
T38484 1240-1242 NN denotes μg
T38486 1242-1243 SYM denotes /
T38487 1243-1245 NN denotes ml
T38488 1245-1246 -RRB- denotes )
T38489 1246-1247 . denotes .
T38490 1247-1343 sentence denotes The solubility of oxygen in the respiration buffer at 25 °C was taken as 246.87 nmol O2 · ml−1.
T38491 1248-1251 DT denotes The
T38492 1252-1262 NN denotes solubility
T38494 1263-1265 IN denotes of
T38495 1266-1272 NN denotes oxygen
T38496 1273-1275 IN denotes in
T38497 1276-1279 DT denotes the
T38499 1280-1291 NN denotes respiration
T38498 1292-1298 NN denotes buffer
T38500 1299-1301 IN denotes at
T38501 1302-1304 CD denotes 25
T38502 1305-1307 NN denotes °C
T38503 1308-1311 VBD denotes was
T38493 1312-1317 VBN denotes taken
T38504 1318-1320 IN denotes as
T38505 1321-1327 CD denotes 246.87
T38506 1328-1332 NN denotes nmol
T38507 1333-1335 NN denotes O2
T38508 1336-1337 SYM denotes ·
T38509 1338-1340 NN denotes ml
T38510 1340-1341 SYM denotes −
T38511 1341-1342 CD denotes 1
T38512 1342-1343 . denotes .
T38513 1343-1405 sentence denotes Respiration rates were expressed as nmol O2 · min−1 · mgdw−1.
T38514 1344-1355 NN denotes Respiration
T38515 1356-1361 NNS denotes rates
T38517 1362-1366 VBD denotes were
T38516 1367-1376 VBN denotes expressed
T38518 1377-1379 IN denotes as
T38519 1380-1384 NN denotes nmol
T38520 1385-1387 NN denotes O2
T38521 1388-1389 SYM denotes ·
T38522 1390-1393 NN denotes min
T38523 1393-1394 SYM denotes −
T38524 1394-1395 CD denotes 1
T38525 1396-1397 SYM denotes ·
T38526 1398-1402 NN denotes mgdw
T38527 1402-1403 SYM denotes −
T38528 1403-1404 CD denotes 1
T38529 1404-1405 . denotes .
R9677 T38202 T38203 amod Mitochondrial,respiration
R9678 T38203 T38204 nsubjpass respiration,assessed
R9679 T38205 T38204 auxpass was,assessed
R9680 T38206 T38204 prep in,assessed
R9681 T38207 T38208 npadvmod saponin,skinned
R9682 T38208 T38210 amod skinned,fibers
R9683 T38209 T38208 punct -,skinned
R9684 T38210 T38206 pobj fibers,in
R9685 T38211 T38210 compound soleus,fibers
R9686 T38212 T38204 prep with,assessed
R9687 T38213 T38212 pobj succinate,with
R9688 T38214 T38213 prep as,succinate
R9689 T38215 T38214 pobj substrate,as
R9690 T38216 T38212 cc and,with
R9691 T38217 T38212 conj in,with
R9692 T38218 T38219 det the,presence
R9693 T38219 T38217 pobj presence,in
R9694 T38220 T38219 prep of,presence
R9695 T38221 T38220 pobj rotenone,of
R9696 T38222 T38223 mark as,described
R9697 T38223 T38204 advcl described,assessed
R9698 T38224 T38223 advmod previously,described
R9699 T38225 T38226 punct [,44
R9700 T38226 T38223 parataxis 44,described
R9701 T38227 T38226 punct ],44
R9702 T38228 T38204 punct .,assessed
R9703 T38230 T38231 prep In,anesthetized
R9704 T38232 T38230 amod brief,In
R9705 T38233 T38231 punct ", ",anesthetized
R9706 T38234 T38235 nummod 3,mo
R9707 T38235 T38237 npadvmod mo,old
R9708 T38236 T38235 punct -,mo
R9709 T38237 T38239 amod old,mice
R9710 T38238 T38237 punct -,old
R9711 T38239 T38231 nsubjpass mice,anesthetized
R9712 T38240 T38239 amod female,mice
R9713 T38241 T38231 auxpass were,anesthetized
R9714 T38242 T38231 prep with,anesthetized
R9715 T38243 T38244 amod chloral,hydrate
R9716 T38244 T38242 pobj hydrate,with
R9717 T38245 T38246 punct (,mg
R9718 T38246 T38244 parataxis mg,hydrate
R9719 T38247 T38246 nummod 400,mg
R9720 T38248 T38249 punct /,kg
R9721 T38249 T38246 prep kg,mg
R9722 T38250 T38249 prep of,kg
R9723 T38251 T38252 compound body,weight
R9724 T38252 T38250 pobj weight,of
R9725 T38253 T38246 punct ),mg
R9726 T38254 T38231 punct .,anesthetized
R9727 T38256 T38257 compound Soleus,fibers
R9728 T38257 T38258 nsubjpass fibers,separated
R9729 T38259 T38258 auxpass were,separated
R9730 T38260 T38258 cc and,separated
R9731 T38261 T38262 advmod then,transferred
R9732 T38262 T38258 conj transferred,separated
R9733 T38263 T38262 prep to,transferred
R9734 T38264 T38265 det a,buffer
R9735 T38265 T38263 pobj buffer,to
R9736 T38266 T38265 punct (,buffer
R9737 T38267 T38268 nummod 2.77,mM
R9738 T38268 T38269 compound mM,EGTA
R9739 T38269 T38265 appos EGTA,buffer
R9740 T38270 T38269 compound K2Ca,EGTA
R9741 T38271 T38269 punct -,EGTA
R9742 T38272 T38269 punct ", ",EGTA
R9743 T38273 T38274 nummod 7.23,mM
R9744 T38274 T38275 compound mM,K2EGTA
R9745 T38275 T38269 appos K2EGTA,EGTA
R9746 T38276 T38269 punct ", ",EGTA
R9747 T38277 T38278 nummod 6.56,mM
R9748 T38278 T38279 compound mM,MgCl2
R9749 T38279 T38269 appos MgCl2,EGTA
R9750 T38280 T38269 punct ", ",EGTA
R9751 T38281 T38282 nummod 20,mM
R9752 T38282 T38283 compound mM,imidazole
R9753 T38283 T38269 appos imidazole,EGTA
R9754 T38284 T38269 punct ", ",EGTA
R9755 T38285 T38286 nummod 53.3,mM
R9756 T38286 T38287 compound mM,MES
R9757 T38287 T38269 appos MES,EGTA
R9758 T38288 T38287 compound K,MES
R9759 T38289 T38287 punct -,MES
R9760 T38290 T38269 punct ", ",EGTA
R9761 T38291 T38292 nummod 20,mM
R9762 T38292 T38293 compound mM,taurine
R9763 T38293 T38269 appos taurine,EGTA
R9764 T38294 T38269 punct ", ",EGTA
R9765 T38295 T38296 nummod 5.3,mM
R9766 T38296 T38297 compound mM,ATP
R9767 T38297 T38269 appos ATP,EGTA
R9768 T38298 T38269 punct ", ",EGTA
R9769 T38299 T38300 nummod 15,mM
R9770 T38300 T38301 compound mM,PCr
R9771 T38301 T38269 appos PCr,EGTA
R9772 T38302 T38269 punct ", ",EGTA
R9773 T38303 T38304 nummod 3,mM
R9774 T38304 T38305 compound mM,KH2PO4
R9775 T38305 T38269 appos KH2PO4,EGTA
R9776 T38306 T38269 punct ", ",EGTA
R9777 T38307 T38308 nummod 0.5,mM
R9778 T38308 T38309 compound mM,DTT
R9779 T38309 T38269 appos DTT,EGTA
R9780 T38310 T38311 punct [,pH
R9781 T38311 T38269 parataxis pH,EGTA
R9782 T38312 T38311 nummod 7.1,pH
R9783 T38313 T38311 punct ],pH
R9784 T38314 T38265 punct ),buffer
R9785 T38315 T38265 acl supplemented,buffer
R9786 T38316 T38315 prep with,supplemented
R9787 T38317 T38318 nummod 50,μg
R9788 T38318 T38316 pobj μg,with
R9789 T38319 T38320 punct /,saponin
R9790 T38320 T38318 prep saponin,μg
R9791 T38321 T38320 compound ml,saponin
R9792 T38322 T38315 cc and,supplemented
R9793 T38323 T38315 conj permeabilized,supplemented
R9794 T38324 T38323 prep for,permeabilized
R9795 T38325 T38326 nummod 30,min
R9796 T38326 T38324 pobj min,for
R9797 T38327 T38323 prep at,permeabilized
R9798 T38328 T38329 nummod 4,°C
R9799 T38329 T38327 pobj °C,at
R9800 T38330 T38323 prep with,permeabilized
R9801 T38331 T38332 amod gentle,stirring
R9802 T38332 T38330 pobj stirring,with
R9803 T38333 T38258 punct .,separated
R9804 T38335 T38336 nsubjpass Fibers,washed
R9805 T38337 T38336 auxpass were,washed
R9806 T38338 T38336 advmod then,washed
R9807 T38339 T38336 advmod twice,washed
R9808 T38340 T38336 prep for,washed
R9809 T38341 T38342 nummod 10,min
R9810 T38342 T38340 pobj min,for
R9811 T38343 T38342 advmod each,min
R9812 T38344 T38345 punct (,EGTA
R9813 T38345 T38336 parataxis EGTA,washed
R9814 T38346 T38347 nummod 2.77,mM
R9815 T38347 T38345 compound mM,EGTA
R9816 T38348 T38345 compound K2Ca,EGTA
R9817 T38349 T38345 punct -,EGTA
R9818 T38350 T38345 punct ", ",EGTA
R9819 T38351 T38352 nummod 7.23,mM
R9820 T38352 T38353 compound mM,K2EGTA
R9821 T38353 T38345 appos K2EGTA,EGTA
R9822 T38354 T38345 punct ", ",EGTA
R9823 T38355 T38356 nummod 1.38,mM
R9824 T38356 T38357 compound mM,MgCl2
R9825 T38357 T38345 appos MgCl2,EGTA
R9826 T38358 T38345 punct ", ",EGTA
R9827 T38359 T38360 nummod 20,mM
R9828 T38360 T38361 compound mM,imidazole
R9829 T38361 T38345 appos imidazole,EGTA
R9830 T38362 T38345 punct ", ",EGTA
R9831 T38363 T38364 nummod 100,mM
R9832 T38364 T38365 compound mM,MES
R9833 T38365 T38345 appos MES,EGTA
R9834 T38366 T38365 compound K,MES
R9835 T38367 T38365 punct -,MES
R9836 T38368 T38345 punct ", ",EGTA
R9837 T38369 T38370 nummod 20,mM
R9838 T38370 T38371 compound mM,taurine
R9839 T38371 T38345 appos taurine,EGTA
R9840 T38372 T38345 punct ", ",EGTA
R9841 T38373 T38374 nummod 3,mM
R9842 T38374 T38375 compound mM,KH2PO4
R9843 T38375 T38345 appos KH2PO4,EGTA
R9844 T38376 T38345 punct ", ",EGTA
R9845 T38377 T38378 nummod 0.5,mM
R9846 T38378 T38379 compound mM,DTT
R9847 T38379 T38345 appos DTT,EGTA
R9848 T38380 T38345 punct ", ",EGTA
R9849 T38381 T38382 nummod 2,mg
R9850 T38382 T38345 appos mg,EGTA
R9851 T38383 T38384 punct /,BSA
R9852 T38384 T38382 prep BSA,mg
R9853 T38385 T38384 compound ml,BSA
R9854 T38386 T38387 punct [,pH
R9855 T38387 T38345 parataxis pH,EGTA
R9856 T38388 T38387 nummod 7.1,pH
R9857 T38389 T38387 punct ],pH
R9858 T38390 T38345 punct ),EGTA
R9859 T38391 T38336 punct .,washed
R9860 T38393 T38394 nsubjpass Respiration,measured
R9861 T38395 T38394 auxpass was,measured
R9862 T38396 T38394 prep at,measured
R9863 T38397 T38398 nummod 25,°C
R9864 T38398 T38396 pobj °C,at
R9865 T38399 T38394 advcl using,measured
R9866 T38400 T38401 det an,probe
R9867 T38401 T38399 dobj probe,using
R9868 T38402 T38401 amod optical,probe
R9869 T38403 T38404 punct (,Probe
R9870 T38404 T38401 parataxis Probe,probe
R9871 T38405 T38404 compound Oxygen,Probe
R9872 T38406 T38404 compound FOXY,Probe
R9873 T38407 T38404 punct ", ",Probe
R9874 T38408 T38409 compound Ocean,Optics
R9875 T38409 T38404 npadvmod Optics,Probe
R9876 T38410 T38404 punct ", ",Probe
R9877 T38411 T38404 npadvmod Dunedin,Probe
R9878 T38412 T38404 punct ", ",Probe
R9879 T38413 T38404 npadvmod Florida,Probe
R9880 T38414 T38404 punct ", ",Probe
R9881 T38415 T38416 compound United,States
R9882 T38416 T38404 npadvmod States,Probe
R9883 T38417 T38404 punct ),Probe
R9884 T38418 T38394 punct .,measured
R9885 T38420 T38421 prep Following,determined
R9886 T38422 T38420 pobj measurement,Following
R9887 T38423 T38422 prep of,measurement
R9888 T38424 T38425 amod basal,respiration
R9889 T38425 T38423 pobj respiration,of
R9890 T38426 T38425 nmod state,respiration
R9891 T38427 T38426 nummod 2,state
R9892 T38428 T38421 punct ", ",determined
R9893 T38429 T38430 amod maximal,respiration
R9894 T38430 T38421 nsubjpass respiration,determined
R9895 T38431 T38430 punct (,respiration
R9896 T38432 T38433 npadvmod ADP,stimulated
R9897 T38433 T38430 amod stimulated,respiration
R9898 T38434 T38433 punct -,stimulated
R9899 T38435 T38430 punct ),respiration
R9900 T38436 T38430 nmod state,respiration
R9901 T38437 T38436 nummod 3,state
R9902 T38438 T38421 auxpass was,determined
R9903 T38439 T38421 prep by,determined
R9904 T38440 T38439 pcomp exposing,by
R9905 T38441 T38440 dobj fibers,exposing
R9906 T38442 T38440 prep to,exposing
R9907 T38443 T38444 nummod 1,mM
R9908 T38444 T38445 compound mM,ADP
R9909 T38445 T38442 pobj ADP,to
R9910 T38446 T38421 punct .,determined
R9911 T38448 T38449 det The,integrity
R9912 T38449 T38450 nsubjpass integrity,assessed
R9913 T38451 T38449 prep of,integrity
R9914 T38452 T38453 det the,membrane
R9915 T38453 T38451 pobj membrane,of
R9916 T38454 T38453 amod outer,membrane
R9917 T38455 T38453 amod mitochondrial,membrane
R9918 T38456 T38450 auxpass was,assessed
R9919 T38457 T38450 prep by,assessed
R9920 T38458 T38457 pcomp adding,by
R9921 T38459 T38460 nummod 8,μM
R9922 T38460 T38461 nmod μM,c
R9923 T38461 T38458 dobj c,adding
R9924 T38462 T38461 amod exogenous,c
R9925 T38463 T38461 compound cytochrome,c
R9926 T38464 T38458 prep to,adding
R9927 T38465 T38466 npadvmod ADP,stimulated
R9928 T38466 T38468 amod stimulated,fibers
R9929 T38467 T38466 punct -,stimulated
R9930 T38468 T38464 pobj fibers,to
R9931 T38469 T38450 punct .,assessed
R9932 T38471 T38472 nmod State,respiration
R9933 T38472 T38474 nsubjpass respiration,evaluated
R9934 T38473 T38471 nummod 4,State
R9935 T38475 T38476 punct (,uncoupled
R9936 T38476 T38472 parataxis uncoupled,respiration
R9937 T38477 T38476 punct ),uncoupled
R9938 T38478 T38474 auxpass was,evaluated
R9939 T38479 T38474 prep following,evaluated
R9940 T38480 T38479 pobj addition,following
R9941 T38481 T38480 prep of,addition
R9942 T38482 T38481 pobj oligomycin,of
R9943 T38483 T38484 punct (,μg
R9944 T38484 T38482 parataxis μg,oligomycin
R9945 T38485 T38484 nummod 1,μg
R9946 T38486 T38487 punct /,ml
R9947 T38487 T38484 prep ml,μg
R9948 T38488 T38484 punct ),μg
R9949 T38489 T38474 punct .,evaluated
R9950 T38491 T38492 det The,solubility
R9951 T38492 T38493 nsubjpass solubility,taken
R9952 T38494 T38492 prep of,solubility
R9953 T38495 T38494 pobj oxygen,of
R9954 T38496 T38492 prep in,solubility
R9955 T38497 T38498 det the,buffer
R9956 T38498 T38496 pobj buffer,in
R9957 T38499 T38498 compound respiration,buffer
R9958 T38500 T38492 prep at,solubility
R9959 T38501 T38502 nummod 25,°C
R9960 T38502 T38500 pobj °C,at
R9961 T38503 T38493 auxpass was,taken
R9962 T38504 T38493 prep as,taken
R9963 T38505 T38506 nummod 246.87,nmol
R9964 T38506 T38507 compound nmol,O2
R9965 T38507 T38504 pobj O2,as
R9966 T38508 T38507 punct ·,O2
R9967 T38509 T38507 appos ml,O2
R9968 T38510 T38511 punct −,1
R9969 T38511 T38509 nummod 1,ml
R9970 T38512 T38493 punct .,taken
R9971 T38514 T38515 compound Respiration,rates
R9972 T38515 T38516 nsubjpass rates,expressed
R9973 T38517 T38516 auxpass were,expressed
R9974 T38518 T38516 prep as,expressed
R9975 T38519 T38520 compound nmol,O2
R9976 T38520 T38518 pobj O2,as
R9977 T38521 T38520 punct ·,O2
R9978 T38522 T38520 appos min,O2
R9979 T38523 T38524 punct −,1
R9980 T38524 T38522 nummod 1,min
R9981 T38525 T38520 punct ·,O2
R9982 T38526 T38520 appos mgdw,O2
R9983 T38527 T38528 punct −,1
R9984 T38528 T38526 nummod 1,mgdw
R9985 T38529 T38516 punct .,expressed