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PMC:1064854 / 6237-10875 JSONTXT 6 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T5681 0-10 NN denotes Disruption
T5682 11-13 IN denotes of
T5683 14-17 DT denotes the
T5685 18-21 NN denotes PGC
T5687 21-22 HYPH denotes -
T5686 22-24 NN denotes 1α
T5684 25-29 NN denotes Gene
T5688 30-32 IN denotes in
T5689 33-37 NNS denotes Mice
T5690 37-141 sentence denotes A neomycin-based gene targeting vector was generated to delete exons 4 and 5 of the murine PGC-1α gene.
T5691 38-39 DT denotes A
T5693 40-48 NN denotes neomycin
T5695 48-49 HYPH denotes -
T5694 49-54 VBN denotes based
T5696 55-59 NN denotes gene
T5697 60-69 NN denotes targeting
T5692 70-76 NN denotes vector
T5699 77-80 VBD denotes was
T5698 81-90 VBN denotes generated
T5700 91-93 TO denotes to
T5701 94-100 VB denotes delete
T5702 101-106 NNS denotes exons
T5703 107-108 CD denotes 4
T5704 109-112 CC denotes and
T5705 113-114 CD denotes 5
T5706 115-117 IN denotes of
T5707 118-121 DT denotes the
T5709 122-128 JJ denotes murine
T5710 129-132 NN denotes PGC
T5712 132-133 HYPH denotes -
T5711 133-135 NN denotes 1α
T5708 136-140 NN denotes gene
T5713 140-141 . denotes .
T5714 141-265 sentence denotes The targeting event resulted in a 3′ homologous recombination with insertion of the remainder of the construct (Figure 1A).
T5715 142-145 DT denotes The
T5717 146-155 NN denotes targeting
T5716 156-161 NN denotes event
T5718 162-170 VBD denotes resulted
T5719 171-173 IN denotes in
T5720 174-175 DT denotes a
T5722 176-177 CD denotes 3
T5723 177-178 SYM denotes ′
T5724 179-189 JJ denotes homologous
T5721 190-203 NN denotes recombination
T5725 204-208 IN denotes with
T5726 209-218 NN denotes insertion
T5727 219-221 IN denotes of
T5728 222-225 DT denotes the
T5729 226-235 NN denotes remainder
T5730 236-238 IN denotes of
T5731 239-242 DT denotes the
T5732 243-252 NN denotes construct
T5733 253-254 -LRB- denotes (
T5735 254-260 NN denotes Figure
T5734 261-263 NN denotes 1A
T5736 263-264 -RRB- denotes )
T5737 264-265 . denotes .
T5738 265-354 sentence denotes The insertion/recombination event was confirmed by Southern blotting and DNA sequencing.
T5739 266-269 DT denotes The
T5741 270-279 NN denotes insertion
T5743 279-280 HYPH denotes /
T5742 280-293 NN denotes recombination
T5740 294-299 NN denotes event
T5745 300-303 VBD denotes was
T5744 304-313 VBN denotes confirmed
T5746 314-316 IN denotes by
T5747 317-325 NNP denotes Southern
T5748 326-334 NN denotes blotting
T5749 335-338 CC denotes and
T5750 339-342 NN denotes DNA
T5751 343-353 NN denotes sequencing
T5752 353-354 . denotes .
T5753 354-515 sentence denotes The insertion caused an exon 3 duplication between exons 5 and 6 that creates a coding region frameshift resulting in a premature termination at amino acid 255.
T5754 355-358 DT denotes The
T5755 359-368 NN denotes insertion
T5756 369-375 VBD denotes caused
T5757 376-378 DT denotes an
T5759 379-383 NN denotes exon
T5760 384-385 CD denotes 3
T5758 386-397 NN denotes duplication
T5761 398-405 IN denotes between
T5762 406-411 NNS denotes exons
T5763 412-413 CD denotes 5
T5764 414-417 CC denotes and
T5765 418-419 CD denotes 6
T5766 420-424 WDT denotes that
T5767 425-432 VBZ denotes creates
T5768 433-434 DT denotes a
T5770 435-441 VBG denotes coding
T5771 442-448 NN denotes region
T5769 449-459 NN denotes frameshift
T5772 460-469 VBG denotes resulting
T5773 470-472 IN denotes in
T5774 473-474 DT denotes a
T5776 475-484 JJ denotes premature
T5775 485-496 NN denotes termination
T5777 497-499 IN denotes at
T5778 500-505 NN denotes amino
T5779 506-510 NN denotes acid
T5780 511-514 CD denotes 255
T5781 514-515 . denotes .
T5782 515-636 sentence denotes Germline transmission of the mutant allele was confirmed using Southern blotting (Figure 1B) and PCR (unpublished data).
T5783 516-524 NN denotes Germline
T5784 525-537 NN denotes transmission
T5786 538-540 IN denotes of
T5787 541-544 DT denotes the
T5789 545-551 NN denotes mutant
T5788 552-558 NN denotes allele
T5790 559-562 VBD denotes was
T5785 563-572 VBN denotes confirmed
T5791 573-578 VBG denotes using
T5792 579-587 NNP denotes Southern
T5793 588-596 NN denotes blotting
T5794 597-598 -LRB- denotes (
T5796 598-604 NN denotes Figure
T5795 605-607 NN denotes 1B
T5797 607-608 -RRB- denotes )
T5798 609-612 CC denotes and
T5799 613-616 NN denotes PCR
T5800 617-618 -LRB- denotes (
T5802 618-629 JJ denotes unpublished
T5801 630-634 NNS denotes data
T5803 634-635 -RRB- denotes )
T5804 635-636 . denotes .
T5805 636-825 sentence denotes The PGC-1α gene disruption resulted in an unstable transcript that could not be detected by RNA blot analysis in heart and other tissues in PGC-1α−/− mice (Figure 1C and unpublished data).
T5806 637-640 DT denotes The
T5808 641-644 NN denotes PGC
T5810 644-645 HYPH denotes -
T5809 645-647 NN denotes 1α
T5811 648-652 NN denotes gene
T5807 653-663 NN denotes disruption
T5812 664-672 VBD denotes resulted
T5813 673-675 IN denotes in
T5814 676-678 DT denotes an
T5816 679-687 JJ denotes unstable
T5815 688-698 NN denotes transcript
T5817 699-703 WDT denotes that
T5819 704-709 MD denotes could
T5820 710-713 RB denotes not
T5821 714-716 VB denotes be
T5818 717-725 VBN denotes detected
T5822 726-728 IN denotes by
T5823 729-732 NN denotes RNA
T5825 733-737 NN denotes blot
T5824 738-746 NN denotes analysis
T5826 747-749 IN denotes in
T5827 750-755 NN denotes heart
T5828 756-759 CC denotes and
T5829 760-765 JJ denotes other
T5830 766-773 NNS denotes tissues
T5831 774-776 IN denotes in
T5832 777-780 NN denotes PGC
T5834 780-781 HYPH denotes -
T5833 781-783 NN denotes 1α
T5836 783-784 SYM denotes −
T5837 784-785 HYPH denotes /
T5838 785-786 SYM denotes −
T5835 787-791 NNS denotes mice
T5839 792-793 -LRB- denotes (
T5841 793-799 NN denotes Figure
T5840 800-802 NN denotes 1C
T5842 803-806 CC denotes and
T5843 807-818 JJ denotes unpublished
T5844 819-823 NNS denotes data
T5845 823-824 -RRB- denotes )
T5846 824-825 . denotes .
T5847 825-914 sentence denotes Quantitative RT-PCR was utilized to further evaluate the efficacy of the gene targeting.
T5848 826-838 JJ denotes Quantitative
T5850 839-841 NN denotes RT
T5851 841-842 HYPH denotes -
T5849 842-845 NN denotes PCR
T5853 846-849 VBD denotes was
T5852 850-858 VBN denotes utilized
T5854 859-861 TO denotes to
T5856 862-869 RB denotes further
T5855 870-878 VB denotes evaluate
T5857 879-882 DT denotes the
T5858 883-891 NN denotes efficacy
T5859 892-894 IN denotes of
T5860 895-898 DT denotes the
T5862 899-903 NN denotes gene
T5861 904-913 NN denotes targeting
T5863 913-914 . denotes .
T5864 914-1160 sentence denotes For these studies, PCR primers were designed to amplify a region of the PGC-1α gene transcript containing the exon 5–6 border (predicted to be absent in PGC-1α−/− mice) or the exon 5–3 border (predicted to be present only in the PGC-1α−/− mice).
T5865 915-918 IN denotes For
T5867 919-924 DT denotes these
T5868 925-932 NNS denotes studies
T5869 932-934 , denotes ,
T5870 934-937 NN denotes PCR
T5871 938-945 NNS denotes primers
T5872 946-950 VBD denotes were
T5866 951-959 VBN denotes designed
T5873 960-962 TO denotes to
T5874 963-970 VB denotes amplify
T5875 971-972 DT denotes a
T5876 973-979 NN denotes region
T5877 980-982 IN denotes of
T5878 983-986 DT denotes the
T5880 987-990 NN denotes PGC
T5882 990-991 HYPH denotes -
T5881 991-993 NN denotes 1α
T5883 994-998 NN denotes gene
T5879 999-1009 NN denotes transcript
T5884 1010-1020 VBG denotes containing
T5885 1021-1024 DT denotes the
T5887 1025-1029 NN denotes exon
T5888 1030-1031 CD denotes 5
T5889 1031-1032 SYM denotes –
T5890 1032-1033 CD denotes 6
T5886 1034-1040 NN denotes border
T5891 1041-1042 -LRB- denotes (
T5892 1042-1051 VBN denotes predicted
T5893 1052-1054 TO denotes to
T5894 1055-1057 VB denotes be
T5895 1058-1064 JJ denotes absent
T5896 1065-1067 IN denotes in
T5897 1068-1071 NN denotes PGC
T5899 1071-1072 HYPH denotes -
T5898 1072-1074 NN denotes 1α
T5901 1074-1075 SYM denotes −
T5902 1075-1076 HYPH denotes /
T5903 1076-1077 SYM denotes −
T5900 1078-1082 NNS denotes mice
T5904 1082-1083 -RRB- denotes )
T5905 1084-1086 CC denotes or
T5906 1087-1090 DT denotes the
T5908 1091-1095 NN denotes exon
T5909 1096-1097 CD denotes 5
T5910 1097-1098 SYM denotes –
T5911 1098-1099 CD denotes 3
T5907 1100-1106 NN denotes border
T5912 1107-1108 -LRB- denotes (
T5913 1108-1117 VBN denotes predicted
T5914 1118-1120 TO denotes to
T5915 1121-1123 VB denotes be
T5916 1124-1131 JJ denotes present
T5917 1132-1136 RB denotes only
T5918 1137-1139 IN denotes in
T5919 1140-1143 DT denotes the
T5921 1144-1147 NN denotes PGC
T5923 1147-1148 HYPH denotes -
T5922 1148-1150 NN denotes 1α
T5924 1150-1151 SYM denotes −
T5925 1151-1152 HYPH denotes /
T5926 1152-1153 SYM denotes −
T5920 1154-1158 NNS denotes mice
T5927 1158-1159 -RRB- denotes )
T5928 1159-1160 . denotes .
T5929 1160-1266 sentence denotes The exon 5–6 amplicon was detected in heart and BAT of wild-type (WT) but not PGC-1α−/− mice (Figure 1D).
T5930 1161-1164 DT denotes The
T5932 1165-1169 NN denotes exon
T5933 1170-1171 CD denotes 5
T5934 1171-1172 SYM denotes –
T5935 1172-1173 CD denotes 6
T5931 1174-1182 NN denotes amplicon
T5937 1183-1186 VBD denotes was
T5936 1187-1195 VBN denotes detected
T5938 1196-1198 IN denotes in
T5939 1199-1204 NN denotes heart
T5940 1205-1208 CC denotes and
T5941 1209-1212 NN denotes BAT
T5942 1213-1215 IN denotes of
T5943 1216-1220 JJ denotes wild
T5945 1220-1221 HYPH denotes -
T5944 1221-1225 NN denotes type
T5947 1226-1227 -LRB- denotes (
T5948 1227-1229 NN denotes WT
T5949 1229-1230 -RRB- denotes )
T5950 1231-1234 CC denotes but
T5951 1235-1238 RB denotes not
T5952 1239-1242 NN denotes PGC
T5954 1242-1243 HYPH denotes -
T5953 1243-1245 NN denotes 1α
T5955 1245-1246 SYM denotes −
T5956 1246-1247 HYPH denotes /
T5957 1247-1248 SYM denotes −
T5946 1249-1253 NNS denotes mice
T5958 1254-1255 -LRB- denotes (
T5960 1255-1261 NN denotes Figure
T5959 1262-1264 NN denotes 1D
T5961 1264-1265 -RRB- denotes )
T5962 1265-1266 . denotes .
T5963 1266-1347 sentence denotes Conversely, the exon 5–3 product was present only in PGC-1α−/− mice (Figure 1D).
T5964 1267-1277 RB denotes Conversely
T5966 1277-1279 , denotes ,
T5967 1279-1282 DT denotes the
T5969 1283-1287 NN denotes exon
T5970 1288-1289 CD denotes 5
T5971 1289-1290 SYM denotes –
T5972 1290-1291 CD denotes 3
T5968 1292-1299 NN denotes product
T5965 1300-1303 VBD denotes was
T5973 1304-1311 JJ denotes present
T5974 1312-1316 RB denotes only
T5975 1317-1319 IN denotes in
T5976 1320-1323 NN denotes PGC
T5978 1323-1324 HYPH denotes -
T5977 1324-1326 NN denotes 1α
T5980 1326-1327 SYM denotes −
T5981 1327-1328 HYPH denotes /
T5982 1328-1329 SYM denotes −
T5979 1330-1334 NNS denotes mice
T5983 1335-1336 -LRB- denotes (
T5985 1336-1342 NN denotes Figure
T5984 1343-1345 NN denotes 1D
T5986 1345-1346 -RRB- denotes )
T5987 1346-1347 . denotes .
T5988 1347-1561 sentence denotes An exon 10–11 border amplicon (predicted to be present in both genotypes) was detected in WT and PGC-1α−/− mice, but was greatly diminished in the PGC-1α−/− mice, indicating that the mutant transcript is unstable.
T5989 1348-1350 DT denotes An
T5991 1351-1355 NN denotes exon
T5992 1356-1358 CD denotes 10
T5993 1358-1359 SYM denotes –
T5994 1359-1361 CD denotes 11
T5995 1362-1368 NN denotes border
T5990 1369-1377 NN denotes amplicon
T5997 1378-1379 -LRB- denotes (
T5998 1379-1388 VBN denotes predicted
T5999 1389-1391 TO denotes to
T6000 1392-1394 VB denotes be
T6001 1395-1402 JJ denotes present
T6002 1403-1405 IN denotes in
T6003 1406-1410 DT denotes both
T6004 1411-1420 NNS denotes genotypes
T6005 1420-1421 -RRB- denotes )
T6006 1422-1425 VBD denotes was
T5996 1426-1434 VBN denotes detected
T6007 1435-1437 IN denotes in
T6008 1438-1440 NN denotes WT
T6010 1441-1444 CC denotes and
T6011 1445-1448 NN denotes PGC
T6013 1448-1449 HYPH denotes -
T6012 1449-1451 NN denotes 1α
T6014 1451-1452 SYM denotes −
T6015 1452-1453 HYPH denotes /
T6016 1453-1454 SYM denotes −
T6009 1455-1459 NNS denotes mice
T6017 1459-1461 , denotes ,
T6018 1461-1464 CC denotes but
T6019 1465-1468 VBD denotes was
T6021 1469-1476 RB denotes greatly
T6020 1477-1487 VBN denotes diminished
T6022 1488-1490 IN denotes in
T6023 1491-1494 DT denotes the
T6025 1495-1498 NN denotes PGC
T6027 1498-1499 HYPH denotes -
T6026 1499-1501 NN denotes 1α
T6028 1501-1502 SYM denotes −
T6029 1502-1503 HYPH denotes /
T6030 1503-1504 SYM denotes −
T6024 1505-1509 NNS denotes mice
T6031 1509-1511 , denotes ,
T6032 1511-1521 VBG denotes indicating
T6033 1522-1526 IN denotes that
T6035 1527-1530 DT denotes the
T6037 1531-1537 NN denotes mutant
T6036 1538-1548 NN denotes transcript
T6034 1549-1551 VBZ denotes is
T6038 1552-1560 JJ denotes unstable
T6039 1560-1561 . denotes .
T6040 1561-1828 sentence denotes PGC-1α protein was not detected in whole cell (Figure 1E) or nuclear protein extracts (unpublished data) isolated from BAT of PGC-1α−/− mice under basal conditions or in response to cold exposure, a condition known to markedly induce the expression of PGC-1α in BAT.
T6041 1562-1565 NN denotes PGC
T6043 1565-1566 HYPH denotes -
T6042 1566-1568 NN denotes 1α
T6044 1569-1576 NN denotes protein
T6046 1577-1580 VBD denotes was
T6047 1581-1584 RB denotes not
T6045 1585-1593 VBN denotes detected
T6048 1594-1596 IN denotes in
T6049 1597-1602 JJ denotes whole
T6050 1603-1607 NN denotes cell
T6052 1608-1609 -LRB- denotes (
T6054 1609-1615 NN denotes Figure
T6053 1616-1618 NN denotes 1E
T6055 1618-1619 -RRB- denotes )
T6056 1620-1622 CC denotes or
T6057 1623-1630 JJ denotes nuclear
T6058 1631-1638 NN denotes protein
T6051 1639-1647 NNS denotes extracts
T6059 1648-1649 -LRB- denotes (
T6061 1649-1660 JJ denotes unpublished
T6060 1661-1665 NNS denotes data
T6062 1665-1666 -RRB- denotes )
T6063 1667-1675 VBN denotes isolated
T6064 1676-1680 IN denotes from
T6065 1681-1684 NN denotes BAT
T6066 1685-1687 IN denotes of
T6067 1688-1691 NN denotes PGC
T6069 1691-1692 HYPH denotes -
T6068 1692-1694 NN denotes 1α
T6071 1694-1695 SYM denotes −
T6072 1695-1696 HYPH denotes /
T6073 1696-1697 SYM denotes −
T6070 1698-1702 NNS denotes mice
T6074 1703-1708 IN denotes under
T6075 1709-1714 JJ denotes basal
T6076 1715-1725 NNS denotes conditions
T6077 1726-1728 CC denotes or
T6078 1729-1731 IN denotes in
T6079 1732-1740 NN denotes response
T6080 1741-1743 IN denotes to
T6081 1744-1748 NN denotes cold
T6082 1749-1757 NN denotes exposure
T6083 1757-1759 , denotes ,
T6084 1759-1760 DT denotes a
T6085 1761-1770 NN denotes condition
T6086 1771-1776 VBN denotes known
T6087 1777-1779 TO denotes to
T6089 1780-1788 RB denotes markedly
T6088 1789-1795 VB denotes induce
T6090 1796-1799 DT denotes the
T6091 1800-1810 NN denotes expression
T6092 1811-1813 IN denotes of
T6093 1814-1817 NN denotes PGC
T6095 1817-1818 HYPH denotes -
T6094 1818-1820 NN denotes 1α
T6096 1821-1823 IN denotes in
T6097 1824-1827 NN denotes BAT
T6098 1827-1828 . denotes .
T6099 1828-1927 sentence denotes Smaller mutant PGC-1α proteins were also not detected by Western blot analysis (unpublished data).
T6100 1829-1836 JJR denotes Smaller
T6102 1837-1843 NN denotes mutant
T6103 1844-1847 NN denotes PGC
T6105 1847-1848 HYPH denotes -
T6104 1848-1850 NN denotes 1α
T6101 1851-1859 NN denotes proteins
T6107 1860-1864 VBD denotes were
T6108 1865-1869 RB denotes also
T6109 1870-1873 RB denotes not
T6106 1874-1882 VBN denotes detected
T6110 1883-1885 IN denotes by
T6111 1886-1893 NNP denotes Western
T6112 1894-1898 NN denotes blot
T6113 1899-1907 NN denotes analysis
T6114 1908-1909 -LRB- denotes (
T6116 1909-1920 JJ denotes unpublished
T6115 1921-1925 NNS denotes data
T6117 1925-1926 -RRB- denotes )
T6118 1926-1927 . denotes .
T6119 1927-2092 sentence denotes Lastly, expression of the genes encoding the other known PGC-1 family members, PGC-1β and PRC, was not significantly altered in heart of PGC-1α−/− mice (Figure 1C).
T6120 1928-1934 RB denotes Lastly
T6122 1934-1936 , denotes ,
T6123 1936-1946 NN denotes expression
T6124 1947-1949 IN denotes of
T6125 1950-1953 DT denotes the
T6126 1954-1959 NNS denotes genes
T6127 1960-1968 VBG denotes encoding
T6128 1969-1972 DT denotes the
T6130 1973-1978 JJ denotes other
T6131 1979-1984 JJ denotes known
T6132 1985-1988 NN denotes PGC
T6133 1988-1989 HYPH denotes -
T6134 1989-1990 CD denotes 1
T6135 1991-1997 NN denotes family
T6129 1998-2005 NNS denotes members
T6136 2005-2007 , denotes ,
T6137 2007-2010 NN denotes PGC
T6139 2010-2011 HYPH denotes -
T6138 2011-2013 NN denotes 1β
T6140 2014-2017 CC denotes and
T6141 2018-2021 NN denotes PRC
T6142 2021-2023 , denotes ,
T6143 2023-2026 VBD denotes was
T6144 2027-2030 RB denotes not
T6145 2031-2044 RB denotes significantly
T6121 2045-2052 VBN denotes altered
T6146 2053-2055 IN denotes in
T6147 2056-2061 NN denotes heart
T6148 2062-2064 IN denotes of
T6149 2065-2068 NN denotes PGC
T6151 2068-2069 HYPH denotes -
T6150 2069-2071 NN denotes 1α
T6153 2071-2072 SYM denotes −
T6154 2072-2073 HYPH denotes /
T6155 2073-2074 SYM denotes −
T6152 2075-2079 NNS denotes mice
T6156 2080-2081 -LRB- denotes (
T6158 2081-2087 NN denotes Figure
T6157 2088-2090 NN denotes 1C
T6159 2090-2091 -RRB- denotes )
T6160 2091-2092 . denotes .
T6161 2092-2209 sentence denotes Taken together, these results support the conclusion that the gene targeting event resulted in a PGC-1α null allele.
T6162 2093-2098 VBN denotes Taken
T6164 2099-2107 RB denotes together
T6165 2107-2109 , denotes ,
T6166 2109-2114 DT denotes these
T6167 2115-2122 NNS denotes results
T6163 2123-2130 VBP denotes support
T6168 2131-2134 DT denotes the
T6169 2135-2145 NN denotes conclusion
T6170 2146-2150 IN denotes that
T6172 2151-2154 DT denotes the
T6174 2155-2159 NN denotes gene
T6175 2160-2169 VBG denotes targeting
T6173 2170-2175 NN denotes event
T6171 2176-2184 VBD denotes resulted
T6176 2185-2187 IN denotes in
T6177 2188-2189 DT denotes a
T6179 2190-2193 NN denotes PGC
T6181 2193-2194 HYPH denotes -
T6180 2194-2196 NN denotes 1α
T6182 2197-2201 JJ denotes null
T6178 2202-2208 NN denotes allele
T6183 2208-2209 . denotes .
R1117 T5682 T5681 prep of,Disruption
R1118 T5683 T5684 det the,Gene
R1119 T5684 T5682 pobj Gene,of
R1120 T5685 T5686 compound PGC,1α
R1121 T5686 T5684 compound 1α,Gene
R1122 T5687 T5686 punct -,1α
R1123 T5688 T5681 prep in,Disruption
R1124 T5689 T5688 pobj Mice,in
R1125 T5691 T5692 det A,vector
R1126 T5692 T5698 nsubjpass vector,generated
R1127 T5693 T5694 npadvmod neomycin,based
R1128 T5694 T5692 amod based,vector
R1129 T5695 T5694 punct -,based
R1130 T5696 T5692 compound gene,vector
R1131 T5697 T5692 compound targeting,vector
R1132 T5699 T5698 auxpass was,generated
R1133 T5700 T5701 aux to,delete
R1134 T5701 T5698 advcl delete,generated
R1135 T5702 T5703 nmod exons,4
R1136 T5703 T5701 dobj 4,delete
R1137 T5704 T5703 cc and,4
R1138 T5705 T5703 conj 5,4
R1139 T5706 T5703 prep of,4
R1140 T5707 T5708 det the,gene
R1141 T5708 T5706 pobj gene,of
R1142 T5709 T5708 amod murine,gene
R1143 T5710 T5711 compound PGC,1α
R1144 T5711 T5708 compound 1α,gene
R1145 T5712 T5711 punct -,1α
R1146 T5713 T5698 punct .,generated
R1147 T5715 T5716 det The,event
R1148 T5716 T5718 nsubj event,resulted
R1149 T5717 T5716 compound targeting,event
R1150 T5719 T5718 prep in,resulted
R1151 T5720 T5721 det a,recombination
R1152 T5721 T5719 pobj recombination,in
R1153 T5722 T5721 nummod 3,recombination
R1154 T5723 T5722 punct ′,3
R1155 T5724 T5721 amod homologous,recombination
R1156 T5725 T5718 prep with,resulted
R1157 T5726 T5725 pobj insertion,with
R1158 T5727 T5726 prep of,insertion
R1159 T5728 T5729 det the,remainder
R1160 T5729 T5727 pobj remainder,of
R1161 T5730 T5729 prep of,remainder
R1162 T5731 T5732 det the,construct
R1163 T5732 T5730 pobj construct,of
R1164 T5733 T5734 punct (,1A
R1165 T5734 T5718 parataxis 1A,resulted
R1166 T5735 T5734 compound Figure,1A
R1167 T5736 T5734 punct ),1A
R1168 T5737 T5718 punct .,resulted
R1169 T5739 T5740 det The,event
R1170 T5740 T5744 nsubjpass event,confirmed
R1171 T5741 T5742 compound insertion,recombination
R1172 T5742 T5740 compound recombination,event
R1173 T5743 T5742 punct /,recombination
R1174 T5745 T5744 auxpass was,confirmed
R1175 T5746 T5744 prep by,confirmed
R1176 T5747 T5748 compound Southern,blotting
R1177 T5748 T5746 pobj blotting,by
R1178 T5749 T5748 cc and,blotting
R1179 T5750 T5751 compound DNA,sequencing
R1180 T5751 T5748 conj sequencing,blotting
R1181 T5752 T5744 punct .,confirmed
R1182 T5754 T5755 det The,insertion
R1183 T5755 T5756 nsubj insertion,caused
R1184 T5757 T5758 det an,duplication
R1185 T5758 T5756 dobj duplication,caused
R1186 T5759 T5758 nmod exon,duplication
R1187 T5760 T5759 nummod 3,exon
R1188 T5761 T5758 prep between,duplication
R1189 T5762 T5763 nmod exons,5
R1190 T5763 T5761 pobj 5,between
R1191 T5764 T5763 cc and,5
R1192 T5765 T5763 conj 6,5
R1193 T5766 T5767 dep that,creates
R1194 T5767 T5758 relcl creates,duplication
R1195 T5768 T5769 det a,frameshift
R1196 T5769 T5767 dobj frameshift,creates
R1197 T5770 T5771 amod coding,region
R1198 T5771 T5769 compound region,frameshift
R1199 T5772 T5767 advcl resulting,creates
R1200 T5773 T5772 prep in,resulting
R1201 T5774 T5775 det a,termination
R1202 T5775 T5773 pobj termination,in
R1203 T5776 T5775 amod premature,termination
R1204 T5777 T5775 prep at,termination
R1205 T5778 T5779 compound amino,acid
R1206 T5779 T5777 pobj acid,at
R1207 T5780 T5779 nummod 255,acid
R1208 T5781 T5756 punct .,caused
R1209 T5783 T5784 compound Germline,transmission
R1210 T5784 T5785 nsubjpass transmission,confirmed
R1211 T5786 T5784 prep of,transmission
R1212 T5787 T5788 det the,allele
R1213 T5788 T5786 pobj allele,of
R1214 T5789 T5788 compound mutant,allele
R1215 T5790 T5785 auxpass was,confirmed
R1216 T5791 T5785 advcl using,confirmed
R1217 T5792 T5793 compound Southern,blotting
R1218 T5793 T5791 dobj blotting,using
R1219 T5794 T5795 punct (,1B
R1220 T5795 T5793 parataxis 1B,blotting
R1221 T5796 T5795 compound Figure,1B
R1222 T5797 T5795 punct ),1B
R1223 T5798 T5793 cc and,blotting
R1224 T5799 T5793 conj PCR,blotting
R1225 T5800 T5801 punct (,data
R1226 T5801 T5799 meta data,PCR
R1227 T5802 T5801 amod unpublished,data
R1228 T5803 T5801 punct ),data
R1229 T5804 T5785 punct .,confirmed
R1230 T5806 T5807 det The,disruption
R1231 T5807 T5812 nsubj disruption,resulted
R1232 T5808 T5809 compound PGC,1α
R1233 T5809 T5807 compound 1α,disruption
R1234 T5810 T5809 punct -,1α
R1235 T5811 T5807 compound gene,disruption
R1236 T5813 T5812 prep in,resulted
R1237 T5814 T5815 det an,transcript
R1238 T5815 T5813 pobj transcript,in
R1239 T5816 T5815 amod unstable,transcript
R1240 T5817 T5818 dep that,detected
R1241 T5818 T5815 relcl detected,transcript
R1242 T5819 T5818 aux could,detected
R1243 T5820 T5818 neg not,detected
R1244 T5821 T5818 auxpass be,detected
R1245 T5822 T5818 prep by,detected
R1246 T5823 T5824 compound RNA,analysis
R1247 T5824 T5822 pobj analysis,by
R1248 T5825 T5824 compound blot,analysis
R1249 T5826 T5818 prep in,detected
R1250 T5827 T5826 pobj heart,in
R1251 T5828 T5827 cc and,heart
R1252 T5829 T5830 amod other,tissues
R1253 T5830 T5827 conj tissues,heart
R1254 T5831 T5818 prep in,detected
R1255 T5832 T5833 nmod PGC,1α
R1256 T5833 T5835 nmod 1α,mice
R1257 T5834 T5833 punct -,1α
R1258 T5835 T5831 pobj mice,in
R1259 T5836 T5833 punct −,1α
R1260 T5837 T5833 punct /,1α
R1261 T5838 T5833 punct −,1α
R1262 T5839 T5840 punct (,1C
R1263 T5840 T5812 parataxis 1C,resulted
R1264 T5841 T5840 compound Figure,1C
R1265 T5842 T5840 cc and,1C
R1266 T5843 T5844 amod unpublished,data
R1267 T5844 T5840 conj data,1C
R1268 T5845 T5840 punct ),1C
R1269 T5846 T5812 punct .,resulted
R1270 T5848 T5849 amod Quantitative,PCR
R1271 T5849 T5852 nsubjpass PCR,utilized
R1272 T5850 T5849 compound RT,PCR
R1273 T5851 T5849 punct -,PCR
R1274 T5853 T5852 auxpass was,utilized
R1275 T5854 T5855 aux to,evaluate
R1276 T5855 T5852 advcl evaluate,utilized
R1277 T5856 T5855 advmod further,evaluate
R1278 T5857 T5858 det the,efficacy
R1279 T5858 T5855 dobj efficacy,evaluate
R1280 T5859 T5858 prep of,efficacy
R1281 T5860 T5861 det the,targeting
R1282 T5861 T5859 pobj targeting,of
R1283 T5862 T5861 compound gene,targeting
R1284 T5863 T5852 punct .,utilized
R1285 T5865 T5866 prep For,designed
R1286 T5867 T5868 det these,studies
R1287 T5868 T5865 pobj studies,For
R1288 T5869 T5866 punct ", ",designed
R1289 T5870 T5871 compound PCR,primers
R1290 T5871 T5866 nsubjpass primers,designed
R1291 T5872 T5866 auxpass were,designed
R1292 T5873 T5874 aux to,amplify
R1293 T5874 T5866 advcl amplify,designed
R1294 T5875 T5876 det a,region
R1295 T5876 T5874 dobj region,amplify
R1296 T5877 T5876 prep of,region
R1297 T5878 T5879 det the,transcript
R1298 T5879 T5877 pobj transcript,of
R1299 T5880 T5881 compound PGC,1α
R1300 T5881 T5879 compound 1α,transcript
R1301 T5882 T5881 punct -,1α
R1302 T5883 T5879 compound gene,transcript
R1303 T5884 T5876 acl containing,region
R1304 T5885 T5886 det the,border
R1305 T5886 T5884 dobj border,containing
R1306 T5887 T5888 nmod exon,5
R1307 T5888 T5886 nummod 5,border
R1308 T5889 T5890 punct –,6
R1309 T5890 T5888 prep 6,5
R1310 T5891 T5886 punct (,border
R1311 T5892 T5886 acl predicted,border
R1312 T5893 T5894 aux to,be
R1313 T5894 T5892 xcomp be,predicted
R1314 T5895 T5894 acomp absent,be
R1315 T5896 T5894 prep in,be
R1316 T5897 T5898 nmod PGC,1α
R1317 T5898 T5900 nmod 1α,mice
R1318 T5899 T5898 punct -,1α
R1319 T5900 T5896 pobj mice,in
R1320 T5901 T5898 punct −,1α
R1321 T5902 T5898 punct /,1α
R1322 T5903 T5898 punct −,1α
R1323 T5904 T5886 punct ),border
R1324 T5905 T5886 cc or,border
R1325 T5906 T5907 det the,border
R1326 T5907 T5886 conj border,border
R1327 T5908 T5909 nmod exon,5
R1328 T5909 T5907 nummod 5,border
R1329 T5910 T5911 punct –,3
R1330 T5911 T5909 prep 3,5
R1331 T5912 T5907 punct (,border
R1332 T5913 T5907 acl predicted,border
R1333 T5914 T5915 aux to,be
R1334 T5915 T5913 xcomp be,predicted
R1335 T5916 T5915 acomp present,be
R1336 T5917 T5918 advmod only,in
R1337 T5918 T5915 prep in,be
R1338 T5919 T5920 det the,mice
R1339 T5920 T5918 pobj mice,in
R1340 T5921 T5922 nmod PGC,1α
R1341 T5922 T5920 nmod 1α,mice
R1342 T5923 T5922 punct -,1α
R1343 T5924 T5922 punct −,1α
R1344 T5925 T5922 punct /,1α
R1345 T5926 T5922 punct −,1α
R1346 T5927 T5907 punct ),border
R1347 T5928 T5866 punct .,designed
R1348 T5930 T5931 det The,amplicon
R1349 T5931 T5936 nsubjpass amplicon,detected
R1350 T5932 T5933 nmod exon,5
R1351 T5933 T5931 nummod 5,amplicon
R1352 T5934 T5935 punct –,6
R1353 T5935 T5933 prep 6,5
R1354 T5937 T5936 auxpass was,detected
R1355 T5938 T5936 prep in,detected
R1356 T5939 T5938 pobj heart,in
R1357 T5940 T5939 cc and,heart
R1358 T5941 T5939 conj BAT,heart
R1359 T5942 T5939 prep of,heart
R1360 T5943 T5944 amod wild,type
R1361 T5944 T5946 nmod type,mice
R1362 T5945 T5944 punct -,type
R1363 T5946 T5942 pobj mice,of
R1364 T5947 T5944 punct (,type
R1365 T5948 T5944 appos WT,type
R1366 T5949 T5944 punct ),type
R1367 T5950 T5944 cc but,type
R1368 T5951 T5950 neg not,but
R1369 T5952 T5953 compound PGC,1α
R1370 T5953 T5944 conj 1α,type
R1371 T5954 T5953 punct -,1α
R1372 T5955 T5953 punct −,1α
R1373 T5956 T5953 punct /,1α
R1374 T5957 T5953 punct −,1α
R1375 T5958 T5959 punct (,1D
R1376 T5959 T5936 parataxis 1D,detected
R1377 T5960 T5959 compound Figure,1D
R1378 T5961 T5959 punct ),1D
R1379 T5962 T5936 punct .,detected
R1380 T5964 T5965 advmod Conversely,was
R1381 T5966 T5965 punct ", ",was
R1382 T5967 T5968 det the,product
R1383 T5968 T5965 nsubj product,was
R1384 T5969 T5970 nmod exon,5
R1385 T5970 T5968 nummod 5,product
R1386 T5971 T5972 punct –,3
R1387 T5972 T5970 prep 3,5
R1388 T5973 T5965 acomp present,was
R1389 T5974 T5975 advmod only,in
R1390 T5975 T5965 prep in,was
R1391 T5976 T5977 nmod PGC,1α
R1392 T5977 T5979 nmod 1α,mice
R1393 T5978 T5977 punct -,1α
R1394 T5979 T5975 pobj mice,in
R1395 T5980 T5977 punct −,1α
R1396 T5981 T5977 punct /,1α
R1397 T5982 T5977 punct −,1α
R1398 T5983 T5984 punct (,1D
R1399 T5984 T5965 parataxis 1D,was
R1400 T5985 T5984 compound Figure,1D
R1401 T5986 T5984 punct ),1D
R1402 T5987 T5965 punct .,was
R1403 T5989 T5990 det An,amplicon
R1404 T5990 T5996 nsubjpass amplicon,detected
R1405 T5991 T5992 nmod exon,10
R1406 T5992 T5990 nummod 10,amplicon
R1407 T5993 T5994 punct –,11
R1408 T5994 T5992 prep 11,10
R1409 T5995 T5990 compound border,amplicon
R1410 T5997 T5990 punct (,amplicon
R1411 T5998 T5990 acl predicted,amplicon
R1412 T5999 T6000 aux to,be
R1413 T6105 T6104 punct -,1α
R1414 T6107 T6106 auxpass were,detected
R1415 T6108 T6106 advmod also,detected
R1416 T6109 T6106 neg not,detected
R1417 T6110 T6106 prep by,detected
R1418 T6111 T6112 compound Western,blot
R1419 T6112 T6113 compound blot,analysis
R1420 T6113 T6110 pobj analysis,by
R1421 T6114 T6115 punct (,data
R1422 T6115 T6106 meta data,detected
R1423 T6116 T6115 amod unpublished,data
R1424 T6117 T6115 punct ),data
R1425 T6118 T6106 punct .,detected
R1426 T6120 T6121 advmod Lastly,altered
R1427 T6000 T5998 xcomp be,predicted
R1428 T6122 T6121 punct ", ",altered
R1429 T6001 T6000 acomp present,be
R1430 T6002 T6000 prep in,be
R1431 T6003 T6004 det both,genotypes
R1432 T6123 T6121 nsubjpass expression,altered
R1433 T6004 T6002 pobj genotypes,in
R1434 T6005 T5996 punct ),detected
R1435 T6006 T5996 auxpass was,detected
R1436 T6007 T5996 prep in,detected
R1437 T6008 T6009 nmod WT,mice
R1438 T6124 T6123 prep of,expression
R1439 T6009 T6007 pobj mice,in
R1440 T6010 T6008 cc and,WT
R1441 T6011 T6012 compound PGC,1α
R1442 T6125 T6126 det the,genes
R1443 T6012 T6008 conj 1α,WT
R1444 T6013 T6012 punct -,1α
R1445 T6014 T6012 punct −,1α
R1446 T6126 T6124 pobj genes,of
R1447 T6015 T6012 punct /,1α
R1448 T6016 T6012 punct −,1α
R1449 T6017 T5996 punct ", ",detected
R1450 T6127 T6126 acl encoding,genes
R1451 T6018 T5996 cc but,detected
R1452 T6019 T6020 auxpass was,diminished
R1453 T6020 T5996 conj diminished,detected
R1454 T6128 T6129 det the,members
R1455 T6021 T6020 advmod greatly,diminished
R1456 T6022 T6020 prep in,diminished
R1457 T6023 T6024 det the,mice
R1458 T6129 T6127 dobj members,encoding
R1459 T6024 T6022 pobj mice,in
R1460 T6025 T6026 nmod PGC,1α
R1461 T6130 T6129 amod other,members
R1462 T6026 T6024 nmod 1α,mice
R1463 T6027 T6026 punct -,1α
R1464 T6028 T6026 punct −,1α
R1465 T6131 T6129 amod known,members
R1466 T6029 T6026 punct /,1α
R1467 T6132 T6129 nmod PGC,members
R1468 T6030 T6026 punct −,1α
R1469 T6031 T6020 punct ", ",diminished
R1470 T6032 T6020 advcl indicating,diminished
R1471 T6033 T6034 mark that,is
R1472 T6133 T6132 punct -,PGC
R1473 T6134 T6132 nummod 1,PGC
R1474 T6034 T6032 ccomp is,indicating
R1475 T6035 T6036 det the,transcript
R1476 T6135 T6129 compound family,members
R1477 T6036 T6034 nsubj transcript,is
R1478 T6037 T6036 compound mutant,transcript
R1479 T6038 T6034 acomp unstable,is
R1480 T6136 T6129 punct ", ",members
R1481 T6039 T5996 punct .,detected
R1482 T6137 T6138 compound PGC,1β
R1483 T6041 T6042 compound PGC,1α
R1484 T6042 T6044 compound 1α,protein
R1485 T6043 T6042 punct -,1α
R1486 T6138 T6129 appos 1β,members
R1487 T6044 T6045 nsubjpass protein,detected
R1488 T6046 T6045 auxpass was,detected
R1489 T6139 T6138 punct -,1β
R1490 T6047 T6045 neg not,detected
R1491 T6048 T6045 prep in,detected
R1492 T6140 T6138 cc and,1β
R1493 T6049 T6050 amod whole,cell
R1494 T6050 T6051 nmod cell,extracts
R1495 T6051 T6048 pobj extracts,in
R1496 T6141 T6138 conj PRC,1β
R1497 T6052 T6053 punct (,1E
R1498 T6053 T6050 parataxis 1E,cell
R1499 T6142 T6121 punct ", ",altered
R1500 T6054 T6053 compound Figure,1E
R1501 T6055 T6053 punct ),1E
R1502 T6056 T6050 cc or,cell
R1503 T6143 T6121 auxpass was,altered
R1504 T6057 T6058 amod nuclear,protein
R1505 T6058 T6050 conj protein,cell
R1506 T6059 T6060 punct (,data
R1507 T6144 T6121 neg not,altered
R1508 T6060 T6051 meta data,extracts
R1509 T6061 T6060 amod unpublished,data
R1510 T6145 T6121 advmod significantly,altered
R1511 T6062 T6060 punct ),data
R1512 T6063 T6051 acl isolated,extracts
R1513 T6146 T6121 prep in,altered
R1514 T6064 T6063 prep from,isolated
R1515 T6065 T6064 pobj BAT,from
R1516 T6066 T6065 prep of,BAT
R1517 T6147 T6146 pobj heart,in
R1518 T6067 T6068 nmod PGC,1α
R1519 T6068 T6070 nmod 1α,mice
R1520 T6148 T6147 prep of,heart
R1521 T6069 T6068 punct -,1α
R1522 T6070 T6066 pobj mice,of
R1523 T6071 T6068 punct −,1α
R1524 T6072 T6068 punct /,1α
R1525 T6073 T6068 punct −,1α
R1526 T6149 T6150 nmod PGC,1α
R1527 T6074 T6063 prep under,isolated
R1528 T6075 T6076 amod basal,conditions
R1529 T6076 T6074 pobj conditions,under
R1530 T6150 T6152 nmod 1α,mice
R1531 T6077 T6045 cc or,detected
R1532 T6078 T6045 conj in,detected
R1533 T6079 T6078 pobj response,in
R1534 T6151 T6150 punct -,1α
R1535 T6080 T6079 prep to,response
R1536 T6081 T6082 compound cold,exposure
R1537 T6082 T6080 pobj exposure,to
R1538 T6152 T6148 pobj mice,of
R1539 T6083 T6082 punct ", ",exposure
R1540 T6084 T6085 det a,condition
R1541 T6085 T6082 appos condition,exposure
R1542 T6153 T6150 punct −,1α
R1543 T6086 T6085 acl known,condition
R1544 T6087 T6088 aux to,induce
R1545 T6154 T6150 punct /,1α
R1546 T6088 T6086 xcomp induce,known
R1547 T6089 T6088 advmod markedly,induce
R1548 T6155 T6150 punct −,1α
R1549 T6090 T6091 det the,expression
R1550 T6091 T6088 dobj expression,induce
R1551 T6092 T6091 prep of,expression
R1552 T6156 T6157 punct (,1C
R1553 T6093 T6094 compound PGC,1α
R1554 T6094 T6092 pobj 1α,of
R1555 T6095 T6094 punct -,1α
R1556 T6096 T6088 prep in,induce
R1557 T6157 T6121 parataxis 1C,altered
R1558 T6097 T6096 pobj BAT,in
R1559 T6098 T6045 punct .,detected
R1560 T6158 T6157 compound Figure,1C
R1561 T6100 T6101 amod Smaller,proteins
R1562 T6101 T6106 nsubjpass proteins,detected
R1563 T6102 T6101 compound mutant,proteins
R1564 T6103 T6104 compound PGC,1α
R1565 T6159 T6157 punct ),1C
R1566 T6104 T6101 compound 1α,proteins
R1567 T6160 T6121 punct .,altered
R1568 T6162 T6163 advcl Taken,support
R1569 T6164 T6162 advmod together,Taken
R1570 T6165 T6163 punct ", ",support
R1571 T6166 T6167 det these,results
R1572 T6167 T6163 nsubj results,support
R1573 T6168 T6169 det the,conclusion
R1574 T6169 T6163 dobj conclusion,support
R1575 T6170 T6171 mark that,resulted
R1576 T6171 T6169 acl resulted,conclusion
R1577 T6172 T6173 det the,event
R1578 T6173 T6171 nsubj event,resulted
R1579 T6174 T6173 nmod gene,event
R1580 T6175 T6174 amod targeting,gene
R1581 T6176 T6171 prep in,resulted
R1582 T6177 T6178 det a,allele
R1583 T6178 T6176 pobj allele,in
R1584 T6179 T6180 compound PGC,1α
R1585 T6180 T6182 npadvmod 1α,null
R1586 T6181 T6180 punct -,1α
R1587 T6182 T6178 amod null,allele
R1588 T6183 T6163 punct .,support