| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T37029 |
0-3 |
IN |
denotes |
For |
| T37031 |
4-12 |
NNP |
denotes |
Southern |
| T37032 |
13-17 |
NN |
denotes |
blot |
| T37033 |
18-25 |
NNS |
denotes |
studies |
| T37034 |
25-27 |
, |
denotes |
, |
| T37035 |
27-28 |
CD |
denotes |
5 |
| T37036 |
29-31 |
NN |
denotes |
μg |
| T37037 |
32-34 |
IN |
denotes |
of |
| T37038 |
35-42 |
JJ |
denotes |
genomic |
| T37039 |
43-46 |
NN |
denotes |
DNA |
| T37040 |
47-50 |
VBD |
denotes |
was |
| T37030 |
51-59 |
VBN |
denotes |
digested |
| T37041 |
60-64 |
IN |
denotes |
with |
| T37042 |
65-69 |
NN |
denotes |
Pst1 |
| T37043 |
70-72 |
CC |
denotes |
or |
| T37044 |
73-77 |
NN |
denotes |
Xba1 |
| T37045 |
77-79 |
, |
denotes |
, |
| T37046 |
79-94 |
VBN |
denotes |
electrophoresed |
| T37047 |
95-97 |
IN |
denotes |
on |
| T37048 |
98-99 |
DT |
denotes |
a |
| T37050 |
100-103 |
CD |
denotes |
0.8 |
| T37051 |
103-104 |
NN |
denotes |
% |
| T37052 |
105-108 |
NN |
denotes |
TAE |
| T37049 |
109-112 |
NN |
denotes |
gel |
| T37053 |
113-116 |
CC |
denotes |
and |
| T37054 |
117-128 |
VBN |
denotes |
transferred |
| T37055 |
129-131 |
IN |
denotes |
to |
| T37056 |
132-133 |
DT |
denotes |
a |
| T37058 |
134-138 |
NN |
denotes |
Gene |
| T37059 |
139-145 |
NN |
denotes |
Screen |
| T37060 |
146-147 |
-LRB- |
denotes |
( |
| T37062 |
147-153 |
NNP |
denotes |
Perkin |
| T37061 |
154-159 |
NNP |
denotes |
Elmer |
| T37063 |
159-161 |
, |
denotes |
, |
| T37064 |
161-170 |
NNP |
denotes |
Wellesley |
| T37065 |
170-172 |
, |
denotes |
, |
| T37066 |
172-182 |
NNP |
denotes |
California |
| T37067 |
182-184 |
, |
denotes |
, |
| T37068 |
184-190 |
NNP |
denotes |
United |
| T37069 |
191-197 |
NNP |
denotes |
States |
| T37070 |
197-198 |
-RRB- |
denotes |
) |
| T37057 |
199-207 |
NN |
denotes |
membrane |
| T37071 |
208-211 |
IN |
denotes |
for |
| T37072 |
212-225 |
NN |
denotes |
hybridization |
| T37073 |
225-226 |
. |
denotes |
. |
| T37074 |
226-398 |
sentence |
denotes |
Western blotting was performed as described [43] using the Enhanced Chemiluminescence detection system (Amersham Pharmacia Biotech, Piscataway, New Jersey, United States). |
| T37075 |
227-234 |
NNP |
denotes |
Western |
| T37076 |
235-243 |
NN |
denotes |
blotting |
| T37078 |
244-247 |
VBD |
denotes |
was |
| T37077 |
248-257 |
VBN |
denotes |
performed |
| T37079 |
258-260 |
IN |
denotes |
as |
| T37080 |
261-270 |
VBN |
denotes |
described |
| T37081 |
271-272 |
-LRB- |
denotes |
[ |
| T37082 |
272-274 |
CD |
denotes |
43 |
| T37083 |
274-275 |
-RRB- |
denotes |
] |
| T37084 |
276-281 |
VBG |
denotes |
using |
| T37085 |
282-285 |
DT |
denotes |
the |
| T37087 |
286-294 |
VBN |
denotes |
Enhanced |
| T37088 |
295-312 |
NN |
denotes |
Chemiluminescence |
| T37089 |
313-322 |
NN |
denotes |
detection |
| T37086 |
323-329 |
NN |
denotes |
system |
| T37090 |
330-331 |
-LRB- |
denotes |
( |
| T37092 |
331-339 |
NNP |
denotes |
Amersham |
| T37093 |
340-349 |
NNP |
denotes |
Pharmacia |
| T37091 |
350-357 |
NNP |
denotes |
Biotech |
| T37094 |
357-359 |
, |
denotes |
, |
| T37095 |
359-369 |
NNP |
denotes |
Piscataway |
| T37096 |
369-371 |
, |
denotes |
, |
| T37097 |
371-374 |
NNP |
denotes |
New |
| T37098 |
375-381 |
NNP |
denotes |
Jersey |
| T37099 |
381-383 |
, |
denotes |
, |
| T37100 |
383-389 |
NNP |
denotes |
United |
| T37101 |
390-396 |
NNP |
denotes |
States |
| T37102 |
396-397 |
-RRB- |
denotes |
) |
| T37103 |
397-398 |
. |
denotes |
. |
| T37104 |
398-500 |
sentence |
denotes |
Ponceau S (Sigma, St. Louis, Missouri, United States) staining of the membrane was used as a control. |
| T37105 |
399-406 |
NNP |
denotes |
Ponceau |
| T37106 |
407-408 |
NNP |
denotes |
S |
| T37108 |
409-410 |
-LRB- |
denotes |
( |
| T37109 |
410-415 |
NNP |
denotes |
Sigma |
| T37110 |
415-417 |
, |
denotes |
, |
| T37111 |
417-420 |
NNP |
denotes |
St. |
| T37112 |
421-426 |
NNP |
denotes |
Louis |
| T37113 |
426-428 |
, |
denotes |
, |
| T37114 |
428-436 |
NNP |
denotes |
Missouri |
| T37115 |
436-438 |
, |
denotes |
, |
| T37116 |
438-444 |
NNP |
denotes |
United |
| T37117 |
445-451 |
NNP |
denotes |
States |
| T37118 |
451-452 |
-RRB- |
denotes |
) |
| T37107 |
453-461 |
NN |
denotes |
staining |
| T37120 |
462-464 |
IN |
denotes |
of |
| T37121 |
465-468 |
DT |
denotes |
the |
| T37122 |
469-477 |
NN |
denotes |
membrane |
| T37123 |
478-481 |
VBD |
denotes |
was |
| T37119 |
482-486 |
VBN |
denotes |
used |
| T37124 |
487-489 |
IN |
denotes |
as |
| T37125 |
490-491 |
DT |
denotes |
a |
| T37126 |
492-499 |
NN |
denotes |
control |
| T37127 |
499-500 |
. |
denotes |
. |
| R9581 |
T37029 |
T37030 |
prep |
For,digested |
| R9582 |
T37031 |
T37032 |
compound |
Southern,blot |
| R9583 |
T37032 |
T37033 |
compound |
blot,studies |
| R9584 |
T37033 |
T37029 |
pobj |
studies,For |
| R9585 |
T37034 |
T37030 |
punct |
", ",digested |
| R9586 |
T37035 |
T37036 |
nummod |
5,μg |
| R9587 |
T37036 |
T37030 |
nsubjpass |
μg,digested |
| R9588 |
T37037 |
T37036 |
prep |
of,μg |
| R9589 |
T37038 |
T37039 |
amod |
genomic,DNA |
| R9590 |
T37039 |
T37037 |
pobj |
DNA,of |
| R9591 |
T37040 |
T37030 |
auxpass |
was,digested |
| R9592 |
T37041 |
T37030 |
prep |
with,digested |
| R9593 |
T37042 |
T37041 |
pobj |
Pst1,with |
| R9594 |
T37043 |
T37042 |
cc |
or,Pst1 |
| R9595 |
T37044 |
T37042 |
conj |
Xba1,Pst1 |
| R9596 |
T37045 |
T37030 |
punct |
", ",digested |
| R9597 |
T37046 |
T37030 |
conj |
electrophoresed,digested |
| R9598 |
T37047 |
T37046 |
prep |
on,electrophoresed |
| R9599 |
T37048 |
T37049 |
det |
a,gel |
| R9600 |
T37049 |
T37047 |
pobj |
gel,on |
| R9601 |
T37050 |
T37051 |
nummod |
0.8,% |
| R9602 |
T37051 |
T37052 |
compound |
%,TAE |
| R9603 |
T37052 |
T37049 |
compound |
TAE,gel |
| R9604 |
T37053 |
T37046 |
cc |
and,electrophoresed |
| R9605 |
T37054 |
T37046 |
conj |
transferred,electrophoresed |
| R9606 |
T37055 |
T37054 |
prep |
to,transferred |
| R9607 |
T37056 |
T37057 |
det |
a,membrane |
| R9608 |
T37057 |
T37055 |
pobj |
membrane,to |
| R9609 |
T37058 |
T37059 |
nmod |
Gene,Screen |
| R9610 |
T37059 |
T37057 |
nmod |
Screen,membrane |
| R9611 |
T37060 |
T37061 |
punct |
(,Elmer |
| R9612 |
T37061 |
T37059 |
parataxis |
Elmer,Screen |
| R9613 |
T37062 |
T37061 |
compound |
Perkin,Elmer |
| R9614 |
T37063 |
T37061 |
punct |
", ",Elmer |
| R9615 |
T37064 |
T37061 |
npadvmod |
Wellesley,Elmer |
| R9616 |
T37065 |
T37061 |
punct |
", ",Elmer |
| R9617 |
T37066 |
T37061 |
npadvmod |
California,Elmer |
| R9618 |
T37067 |
T37061 |
punct |
", ",Elmer |
| R9619 |
T37068 |
T37069 |
compound |
United,States |
| R9620 |
T37069 |
T37061 |
npadvmod |
States,Elmer |
| R9621 |
T37070 |
T37061 |
punct |
),Elmer |
| R9622 |
T37071 |
T37054 |
prep |
for,transferred |
| R9623 |
T37072 |
T37071 |
pobj |
hybridization,for |
| R9624 |
T37073 |
T37030 |
punct |
.,digested |
| R9625 |
T37075 |
T37076 |
compound |
Western,blotting |
| R9626 |
T37076 |
T37077 |
nsubjpass |
blotting,performed |
| R9627 |
T37078 |
T37077 |
auxpass |
was,performed |
| R9628 |
T37079 |
T37080 |
mark |
as,described |
| R9629 |
T37080 |
T37077 |
advcl |
described,performed |
| R9630 |
T37081 |
T37082 |
punct |
[,43 |
| R9631 |
T37082 |
T37080 |
parataxis |
43,described |
| R9632 |
T37083 |
T37082 |
punct |
],43 |
| R9633 |
T37084 |
T37077 |
advcl |
using,performed |
| R9634 |
T37085 |
T37086 |
det |
the,system |
| R9635 |
T37086 |
T37084 |
dobj |
system,using |
| R9636 |
T37087 |
T37088 |
amod |
Enhanced,Chemiluminescence |
| R9637 |
T37088 |
T37086 |
compound |
Chemiluminescence,system |
| R9638 |
T37089 |
T37086 |
compound |
detection,system |
| R9639 |
T37090 |
T37091 |
punct |
(,Biotech |
| R9640 |
T37091 |
T37086 |
parataxis |
Biotech,system |
| R9641 |
T37092 |
T37091 |
compound |
Amersham,Biotech |
| R9642 |
T37093 |
T37091 |
compound |
Pharmacia,Biotech |
| R9643 |
T37094 |
T37091 |
punct |
", ",Biotech |
| R9644 |
T37095 |
T37091 |
npadvmod |
Piscataway,Biotech |
| R9645 |
T37096 |
T37091 |
punct |
", ",Biotech |
| R9646 |
T37097 |
T37098 |
compound |
New,Jersey |
| R9647 |
T37098 |
T37091 |
npadvmod |
Jersey,Biotech |
| R9648 |
T37099 |
T37091 |
punct |
", ",Biotech |
| R9649 |
T37100 |
T37101 |
compound |
United,States |
| R9650 |
T37101 |
T37091 |
npadvmod |
States,Biotech |
| R9651 |
T37102 |
T37091 |
punct |
),Biotech |
| R9652 |
T37103 |
T37077 |
punct |
.,performed |
| R9653 |
T37105 |
T37106 |
nmod |
Ponceau,S |
| R9654 |
T37106 |
T37107 |
nmod |
S,staining |
| R9655 |
T37107 |
T37119 |
nsubjpass |
staining,used |
| R9656 |
T37108 |
T37109 |
punct |
(,Sigma |
| R9657 |
T37109 |
T37106 |
parataxis |
Sigma,S |
| R9658 |
T37110 |
T37109 |
punct |
", ",Sigma |
| R9659 |
T37111 |
T37112 |
compound |
St.,Louis |
| R9660 |
T37112 |
T37109 |
npadvmod |
Louis,Sigma |
| R9661 |
T37113 |
T37109 |
punct |
", ",Sigma |
| R9662 |
T37114 |
T37109 |
npadvmod |
Missouri,Sigma |
| R9663 |
T37115 |
T37109 |
punct |
", ",Sigma |
| R9664 |
T37116 |
T37117 |
compound |
United,States |
| R9665 |
T37117 |
T37109 |
npadvmod |
States,Sigma |
| R9666 |
T37118 |
T37109 |
punct |
),Sigma |
| R9667 |
T37120 |
T37107 |
prep |
of,staining |
| R9668 |
T37121 |
T37122 |
det |
the,membrane |
| R9669 |
T37122 |
T37120 |
pobj |
membrane,of |
| R9670 |
T37123 |
T37119 |
auxpass |
was,used |
| R9671 |
T37124 |
T37119 |
prep |
as,used |
| R9672 |
T37125 |
T37126 |
det |
a,control |
| R9673 |
T37126 |
T37124 |
pobj |
control,as |
| R9674 |
T37127 |
T37119 |
punct |
.,used |
