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PMC:1064854 / 61048-62177 JSONTXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T36782 0-5 JJ denotes Total
T36783 6-9 NN denotes RNA
T36785 10-13 VBD denotes was
T36784 14-22 VBN denotes isolated
T36786 23-25 IN denotes by
T36787 26-29 DT denotes the
T36789 30-36 NN denotes RNAzol
T36788 37-43 NN denotes method
T36790 44-45 -LRB- denotes (
T36792 45-48 NNP denotes Tel
T36793 48-49 HYPH denotes -
T36791 49-53 NNP denotes Test
T36794 53-55 , denotes ,
T36795 55-66 NNP denotes Friendswood
T36796 66-68 , denotes ,
T36797 68-73 NNP denotes Texas
T36798 73-74 -RRB- denotes )
T36799 75-78 CC denotes and
T36800 79-87 NNP denotes Northern
T36801 88-96 NN denotes blotting
T36803 97-100 VBD denotes was
T36802 101-110 VBN denotes performed
T36804 111-113 IN denotes as
T36806 114-124 RB denotes previously
T36805 125-134 VBN denotes described
T36807 135-136 -LRB- denotes [
T36808 136-138 CD denotes 40
T36809 138-139 -RRB- denotes ]
T36810 139-140 . denotes .
T36811 140-244 sentence denotes The PGC-1β and PRC cDNAs were generous gifts from Bruce Spiegelman and Richard Scarpulla, respectively.
T36812 141-144 DT denotes The
T36814 145-148 NN denotes PGC
T36816 148-149 HYPH denotes -
T36815 149-151 NN denotes 1β
T36817 152-155 CC denotes and
T36818 156-159 NN denotes PRC
T36813 160-165 NNS denotes cDNAs
T36819 166-170 VBD denotes were
T36820 171-179 JJ denotes generous
T36821 180-185 NNS denotes gifts
T36822 186-190 IN denotes from
T36823 191-196 NNP denotes Bruce
T36824 197-207 NNP denotes Spiegelman
T36825 208-211 CC denotes and
T36826 212-219 NNP denotes Richard
T36827 220-229 NNP denotes Scarpulla
T36828 229-231 , denotes ,
T36829 231-243 RB denotes respectively
T36830 243-244 . denotes .
T36831 244-292 sentence denotes The UCP-1 cDNA was a gift from Daniel Ricquier.
T36832 245-248 DT denotes The
T36834 249-252 NN denotes UCP
T36835 252-253 HYPH denotes -
T36836 253-254 CD denotes 1
T36833 255-259 NN denotes cDNA
T36837 260-263 VBD denotes was
T36838 264-265 DT denotes a
T36839 266-270 NN denotes gift
T36840 271-275 IN denotes from
T36841 276-282 NNP denotes Daniel
T36842 283-291 NNP denotes Ricquier
T36843 291-292 . denotes .
T36844 292-332 sentence denotes RT-PCR was performed as described [41].
T36845 293-295 NN denotes RT
T36847 295-296 HYPH denotes -
T36846 296-299 NN denotes PCR
T36849 300-303 VBD denotes was
T36848 304-313 VBN denotes performed
T36850 314-316 IN denotes as
T36851 317-326 VBN denotes described
T36852 327-328 -LRB- denotes [
T36853 328-330 CD denotes 41
T36854 330-331 -RRB- denotes ]
T36855 331-332 . denotes .
T36856 332-565 sentence denotes In brief, total RNA isolated from soleus muscle, BAT, and heart of 1–2-mo-old PGC-1α+/+ or PGCα−/− mice was reverse transcribed with Taqman reverse transcription reagents (Applied Biosystems, Foster City, California, United States).
T36857 333-335 IN denotes In
T36859 336-341 JJ denotes brief
T36860 341-343 , denotes ,
T36861 343-348 JJ denotes total
T36862 349-352 NN denotes RNA
T36863 353-361 VBN denotes isolated
T36864 362-366 IN denotes from
T36865 367-373 NN denotes soleus
T36866 374-380 NN denotes muscle
T36867 380-382 , denotes ,
T36868 382-385 NN denotes BAT
T36869 385-387 , denotes ,
T36870 387-390 CC denotes and
T36871 391-396 NN denotes heart
T36872 397-399 IN denotes of
T36873 400-401 CD denotes 1
T36875 401-402 SYM denotes –
T36874 402-403 CD denotes 2
T36877 403-404 HYPH denotes -
T36876 404-406 NN denotes mo
T36879 406-407 HYPH denotes -
T36878 407-410 JJ denotes old
T36881 411-414 NN denotes PGC
T36883 414-415 HYPH denotes -
T36882 415-417 NN denotes 1α
T36884 417-418 SYM denotes +
T36885 418-419 HYPH denotes /
T36886 419-420 SYM denotes +
T36887 421-423 CC denotes or
T36888 424-428 NN denotes PGCα
T36889 428-429 SYM denotes −
T36890 429-430 HYPH denotes /
T36891 430-431 SYM denotes −
T36880 432-436 NNS denotes mice
T36892 437-440 VBD denotes was
T36893 441-448 RB denotes reverse
T36858 449-460 VBN denotes transcribed
T36894 461-465 IN denotes with
T36895 466-472 NNP denotes Taqman
T36897 473-480 JJ denotes reverse
T36898 481-494 NN denotes transcription
T36896 495-503 NNS denotes reagents
T36899 504-505 -LRB- denotes (
T36901 505-512 NNP denotes Applied
T36900 513-523 NNP denotes Biosystems
T36902 523-525 , denotes ,
T36903 525-531 NNP denotes Foster
T36904 532-536 NNP denotes City
T36905 536-538 , denotes ,
T36906 538-548 NNP denotes California
T36907 548-550 , denotes ,
T36908 550-556 NNP denotes United
T36909 557-563 NNP denotes States
T36910 563-564 -RRB- denotes )
T36911 564-565 . denotes .
T36912 565-706 sentence denotes Reactions were performed in triplicate in 96-well format using Taqman core reagents and a Prism 7500 Sequence Detector (Applied Biosystems).
T36913 566-575 NNS denotes Reactions
T36915 576-580 VBD denotes were
T36914 581-590 VBN denotes performed
T36916 591-593 IN denotes in
T36917 594-604 NN denotes triplicate
T36918 605-607 IN denotes in
T36919 608-610 CD denotes 96
T36921 610-611 HYPH denotes -
T36920 611-615 NN denotes well
T36922 616-622 NN denotes format
T36923 623-628 VBG denotes using
T36924 629-635 NNP denotes Taqman
T36926 636-640 NN denotes core
T36925 641-649 NNS denotes reagents
T36927 650-653 CC denotes and
T36928 654-655 DT denotes a
T36930 656-661 NN denotes Prism
T36931 662-666 CD denotes 7500
T36932 667-675 NN denotes Sequence
T36929 676-684 NN denotes Detector
T36933 685-686 -LRB- denotes (
T36935 686-693 NNP denotes Applied
T36934 694-704 NNP denotes Biosystems
T36936 704-705 -RRB- denotes )
T36937 705-706 . denotes .
T36938 706-809 sentence denotes The mouse-specific primer-probe sets used to detect specific gene expression can be found in Table S1.
T36939 707-710 DT denotes The
T36941 711-716 NN denotes mouse
T36943 716-717 HYPH denotes -
T36942 717-725 JJ denotes specific
T36944 726-732 NN denotes primer
T36946 732-733 HYPH denotes -
T36945 733-738 NN denotes probe
T36940 739-743 NNS denotes sets
T36948 744-748 VBN denotes used
T36949 749-751 TO denotes to
T36950 752-758 VB denotes detect
T36951 759-767 JJ denotes specific
T36953 768-772 NN denotes gene
T36952 773-783 NN denotes expression
T36954 784-787 MD denotes can
T36955 788-790 VB denotes be
T36947 791-796 VBN denotes found
T36956 797-799 IN denotes in
T36957 800-805 NN denotes Table
T36958 806-808 NN denotes S1
T36959 808-809 . denotes .
T36960 809-868 sentence denotes The primers for UCP-1 have been previously described [42].
T36961 810-813 DT denotes The
T36962 814-821 NNS denotes primers
T36964 822-825 IN denotes for
T36965 826-829 NN denotes UCP
T36966 829-830 HYPH denotes -
T36967 830-831 CD denotes 1
T36968 832-836 VBP denotes have
T36969 837-841 VBN denotes been
T36970 842-852 RB denotes previously
T36963 853-862 VBN denotes described
T36971 863-864 -LRB- denotes [
T36972 864-866 CD denotes 42
T36973 866-867 -RRB- denotes ]
T36974 867-868 . denotes .
T36975 868-1015 sentence denotes Actin primer-probe set (Applied Biosystems) was included in a separate well and used to normalize the soleus, BAT, and heart gene expression data.
T36976 869-874 NN denotes Actin
T36978 875-881 NN denotes primer
T36980 881-882 HYPH denotes -
T36979 882-887 NN denotes probe
T36977 888-891 NN denotes set
T36982 892-893 -LRB- denotes (
T36984 893-900 NNP denotes Applied
T36983 901-911 NNP denotes Biosystems
T36985 911-912 -RRB- denotes )
T36986 913-916 VBD denotes was
T36981 917-925 VBN denotes included
T36987 926-928 IN denotes in
T36988 929-930 DT denotes a
T36990 931-939 JJ denotes separate
T36989 940-944 NN denotes well
T36991 945-948 CC denotes and
T36992 949-953 VBN denotes used
T36993 954-956 TO denotes to
T36994 957-966 VB denotes normalize
T36995 967-970 DT denotes the
T36997 971-977 NN denotes soleus
T36998 977-979 , denotes ,
T36999 979-982 NN denotes BAT
T37000 982-984 , denotes ,
T37001 984-987 CC denotes and
T37002 988-993 NN denotes heart
T37003 994-998 NN denotes gene
T37004 999-1009 NN denotes expression
T36996 1010-1014 NNS denotes data
T37005 1014-1015 . denotes .
T37006 1015-1129 sentence denotes GAPDH Rodent primers (Applied Biosystems) were used in the same well to normalize the liver gene expression data.
T37007 1016-1021 NN denotes GAPDH
T37009 1022-1028 NN denotes Rodent
T37008 1029-1036 NNS denotes primers
T37011 1037-1038 -LRB- denotes (
T37013 1038-1045 NNP denotes Applied
T37012 1046-1056 NNP denotes Biosystems
T37014 1056-1057 -RRB- denotes )
T37015 1058-1062 VBD denotes were
T37010 1063-1067 VBN denotes used
T37016 1068-1070 IN denotes in
T37017 1071-1074 DT denotes the
T37019 1075-1079 JJ denotes same
T37018 1080-1084 NN denotes well
T37020 1085-1087 TO denotes to
T37021 1088-1097 VB denotes normalize
T37022 1098-1101 DT denotes the
T37024 1102-1107 NN denotes liver
T37025 1108-1112 NN denotes gene
T37026 1113-1123 NN denotes expression
T37023 1124-1128 NNS denotes data
T37027 1128-1129 . denotes .
R9354 T36782 T36783 amod Total,RNA
R9355 T36783 T36784 nsubjpass RNA,isolated
R9356 T36785 T36784 auxpass was,isolated
R9357 T36786 T36784 prep by,isolated
R9358 T36787 T36788 det the,method
R9359 T36788 T36786 pobj method,by
R9360 T36789 T36788 compound RNAzol,method
R9361 T36790 T36791 punct (,Test
R9362 T36791 T36788 parataxis Test,method
R9363 T36792 T36791 compound Tel,Test
R9364 T36793 T36791 punct -,Test
R9365 T36794 T36791 punct ", ",Test
R9366 T36795 T36791 npadvmod Friendswood,Test
R9367 T36796 T36791 punct ", ",Test
R9368 T36797 T36791 npadvmod Texas,Test
R9369 T36798 T36791 punct ),Test
R9370 T36799 T36784 cc and,isolated
R9371 T36800 T36801 compound Northern,blotting
R9372 T36801 T36802 nsubjpass blotting,performed
R9373 T36802 T36784 conj performed,isolated
R9374 T36803 T36802 auxpass was,performed
R9375 T36804 T36805 mark as,described
R9376 T36805 T36802 advcl described,performed
R9377 T36806 T36805 advmod previously,described
R9378 T36807 T36808 punct [,40
R9379 T36808 T36805 parataxis 40,described
R9380 T36809 T36808 punct ],40
R9381 T36810 T36802 punct .,performed
R9382 T36812 T36813 det The,cDNAs
R9383 T36813 T36819 nsubj cDNAs,were
R9384 T36814 T36815 nmod PGC,1β
R9385 T36815 T36813 nmod 1β,cDNAs
R9386 T36816 T36815 punct -,1β
R9387 T36817 T36815 cc and,1β
R9388 T36818 T36815 conj PRC,1β
R9389 T36820 T36821 amod generous,gifts
R9390 T36821 T36819 attr gifts,were
R9391 T36822 T36821 prep from,gifts
R9392 T36823 T36824 compound Bruce,Spiegelman
R9393 T36824 T36822 pobj Spiegelman,from
R9394 T36825 T36824 cc and,Spiegelman
R9395 T36826 T36827 compound Richard,Scarpulla
R9396 T36827 T36824 conj Scarpulla,Spiegelman
R9397 T36828 T36819 punct ", ",were
R9398 T36829 T36819 advmod respectively,were
R9399 T36830 T36819 punct .,were
R9400 T36832 T36833 det The,cDNA
R9401 T36833 T36837 nsubj cDNA,was
R9402 T36834 T36833 nmod UCP,cDNA
R9403 T36835 T36834 punct -,UCP
R9404 T36836 T36834 nummod 1,UCP
R9405 T36838 T36839 det a,gift
R9406 T36839 T36837 attr gift,was
R9407 T36840 T36839 prep from,gift
R9408 T36841 T36842 compound Daniel,Ricquier
R9409 T36842 T36840 pobj Ricquier,from
R9410 T36843 T36837 punct .,was
R9411 T36845 T36846 compound RT,PCR
R9412 T36846 T36848 nsubjpass PCR,performed
R9413 T36847 T36846 punct -,PCR
R9414 T36849 T36848 auxpass was,performed
R9415 T36850 T36851 mark as,described
R9416 T36851 T36848 advcl described,performed
R9417 T36852 T36853 punct [,41
R9418 T36853 T36851 parataxis 41,described
R9419 T36854 T36853 punct ],41
R9420 T36855 T36848 punct .,performed
R9421 T36857 T36858 prep In,transcribed
R9422 T36859 T36857 amod brief,In
R9423 T36860 T36858 punct ", ",transcribed
R9424 T36861 T36862 amod total,RNA
R9425 T36862 T36858 nsubjpass RNA,transcribed
R9426 T36863 T36862 acl isolated,RNA
R9427 T36864 T36863 prep from,isolated
R9428 T36865 T36866 compound soleus,muscle
R9429 T36866 T36864 pobj muscle,from
R9430 T36867 T36866 punct ", ",muscle
R9431 T36868 T36866 conj BAT,muscle
R9432 T36869 T36868 punct ", ",BAT
R9433 T36870 T36868 cc and,BAT
R9434 T36871 T36868 conj heart,BAT
R9435 T36872 T36866 prep of,muscle
R9436 T36873 T36874 quantmod 1,2
R9437 T36874 T36876 nummod 2,mo
R9438 T36875 T36874 punct –,2
R9439 T36876 T36878 npadvmod mo,old
R9440 T36877 T36876 punct -,mo
R9441 T36878 T36880 amod old,mice
R9442 T36879 T36878 punct -,old
R9443 T36880 T36872 pobj mice,of
R9444 T36881 T36882 nmod PGC,1α
R9445 T36882 T36880 nmod 1α,mice
R9446 T36883 T36882 punct -,1α
R9447 T36884 T36882 punct +,1α
R9448 T36885 T36882 punct /,1α
R9449 T36886 T36882 punct +,1α
R9450 T36887 T36882 cc or,1α
R9451 T36888 T36882 conj PGCα,1α
R9452 T36889 T36888 punct −,PGCα
R9453 T36890 T36888 punct /,PGCα
R9454 T36891 T36888 punct −,PGCα
R9455 T36892 T36858 auxpass was,transcribed
R9456 T36893 T36858 advmod reverse,transcribed
R9457 T36894 T36858 prep with,transcribed
R9458 T36895 T36896 nmod Taqman,reagents
R9459 T36896 T36894 pobj reagents,with
R9460 T36897 T36898 amod reverse,transcription
R9461 T36898 T36896 compound transcription,reagents
R9462 T36899 T36900 punct (,Biosystems
R9463 T36900 T36896 parataxis Biosystems,reagents
R9464 T36901 T36900 compound Applied,Biosystems
R9465 T36902 T36900 punct ", ",Biosystems
R9466 T36903 T36904 compound Foster,City
R9467 T36904 T36900 npadvmod City,Biosystems
R9468 T36905 T36900 punct ", ",Biosystems
R9469 T36906 T36900 npadvmod California,Biosystems
R9470 T36907 T36900 punct ", ",Biosystems
R9471 T36908 T36909 compound United,States
R9472 T36909 T36900 npadvmod States,Biosystems
R9473 T36910 T36900 punct ),Biosystems
R9474 T36911 T36858 punct .,transcribed
R9475 T36913 T36914 nsubjpass Reactions,performed
R9476 T36915 T36914 auxpass were,performed
R9477 T36916 T36914 prep in,performed
R9478 T36917 T36916 pobj triplicate,in
R9479 T36918 T36914 prep in,performed
R9480 T36919 T36920 nummod 96,well
R9481 T36920 T36922 compound well,format
R9482 T36921 T36920 punct -,well
R9483 T36922 T36918 pobj format,in
R9484 T36923 T36914 advcl using,performed
R9485 T36924 T36925 compound Taqman,reagents
R9486 T36925 T36923 dobj reagents,using
R9487 T36926 T36925 compound core,reagents
R9488 T36927 T36925 cc and,reagents
R9489 T36928 T36929 det a,Detector
R9490 T36929 T36925 conj Detector,reagents
R9491 T36930 T36929 nmod Prism,Detector
R9492 T36931 T36930 nummod 7500,Prism
R9493 T36932 T36929 compound Sequence,Detector
R9494 T36933 T36934 punct (,Biosystems
R9495 T36934 T36929 parataxis Biosystems,Detector
R9496 T36935 T36934 compound Applied,Biosystems
R9497 T36936 T36934 punct ),Biosystems
R9498 T36937 T36914 punct .,performed
R9499 T36939 T36940 det The,sets
R9500 T36940 T36947 nsubjpass sets,found
R9501 T36941 T36942 npadvmod mouse,specific
R9502 T36942 T36940 amod specific,sets
R9503 T36943 T36942 punct -,specific
R9504 T36944 T36945 compound primer,probe
R9505 T36945 T36940 compound probe,sets
R9506 T36946 T36945 punct -,probe
R9507 T36948 T36940 acl used,sets
R9508 T36949 T36950 aux to,detect
R9509 T36950 T36948 advcl detect,used
R9510 T36951 T36952 amod specific,expression
R9511 T36952 T36950 dobj expression,detect
R9512 T36953 T36952 compound gene,expression
R9513 T36954 T36947 aux can,found
R9514 T36955 T36947 auxpass be,found
R9515 T36956 T36947 prep in,found
R9516 T36957 T36958 compound Table,S1
R9517 T36958 T36956 pobj S1,in
R9518 T36959 T36947 punct .,found
R9519 T36961 T36962 det The,primers
R9520 T36962 T36963 nsubjpass primers,described
R9521 T36964 T36962 prep for,primers
R9522 T36965 T36964 pobj UCP,for
R9523 T36966 T36965 punct -,UCP
R9524 T36967 T36965 nummod 1,UCP
R9525 T36968 T36963 aux have,described
R9526 T36969 T36963 auxpass been,described
R9527 T36970 T36963 advmod previously,described
R9528 T36971 T36972 punct [,42
R9529 T36972 T36963 parataxis 42,described
R9530 T36973 T36972 punct ],42
R9531 T36974 T36963 punct .,described
R9532 T36976 T36977 compound Actin,set
R9533 T36977 T36981 nsubjpass set,included
R9534 T36978 T36979 compound primer,probe
R9535 T36979 T36977 compound probe,set
R9536 T36980 T36979 punct -,probe
R9537 T36982 T36983 punct (,Biosystems
R9538 T36983 T36977 parataxis Biosystems,set
R9539 T36984 T36983 compound Applied,Biosystems
R9540 T36985 T36983 punct ),Biosystems
R9541 T36986 T36981 auxpass was,included
R9542 T36987 T36981 prep in,included
R9543 T36988 T36989 det a,well
R9544 T36989 T36987 pobj well,in
R9545 T36990 T36989 amod separate,well
R9546 T36991 T36981 cc and,included
R9547 T36992 T36981 conj used,included
R9548 T36993 T36994 aux to,normalize
R9549 T36994 T36992 advcl normalize,used
R9550 T36995 T36996 det the,data
R9551 T36996 T36994 dobj data,normalize
R9552 T36997 T36996 nmod soleus,data
R9553 T36998 T36997 punct ", ",soleus
R9554 T36999 T36997 conj BAT,soleus
R9555 T37000 T36999 punct ", ",BAT
R9556 T37001 T36999 cc and,BAT
R9557 T37002 T37003 compound heart,gene
R9558 T37003 T36999 conj gene,BAT
R9559 T37004 T36996 compound expression,data
R9560 T37005 T36981 punct .,included
R9561 T37007 T37008 compound GAPDH,primers
R9562 T37008 T37010 nsubjpass primers,used
R9563 T37009 T37008 compound Rodent,primers
R9564 T37011 T37012 punct (,Biosystems
R9565 T37012 T37008 parataxis Biosystems,primers
R9566 T37013 T37012 compound Applied,Biosystems
R9567 T37014 T37012 punct ),Biosystems
R9568 T37015 T37010 auxpass were,used
R9569 T37016 T37010 prep in,used
R9570 T37017 T37018 det the,well
R9571 T37018 T37016 pobj well,in
R9572 T37019 T37018 amod same,well
R9573 T37020 T37021 aux to,normalize
R9574 T37021 T37010 advcl normalize,used
R9575 T37022 T37023 det the,data
R9576 T37023 T37021 dobj data,normalize
R9577 T37024 T37025 compound liver,gene
R9578 T37025 T37023 compound gene,data
R9579 T37026 T37023 compound expression,data
R9580 T37027 T37010 punct .,used